LOCUS       DAJTYZ010000001        88079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000001 DAJTYZ010000000
VERSION     DAJTYZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.001"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="OO596_000001"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="OO596_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6715985.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="OO596_000002"
     tRNA            1230..1305
                     /locus_tag="OO596_000002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="OO596_000003"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="OO596_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="OO596_000004"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="OO596_000004"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6715986.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="OO596_000005"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="OO596_000005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6715987.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="OO596_000006"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="OO596_000006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6715988.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="OO596_000007"
     CDS             complement(4869..5306)
                     /locus_tag="OO596_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCR6715989.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="OO596_000008"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="OO596_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="OO596_000009"
     CDS             complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="OO596_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HCR6715990.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7988..8272
                     /gene="pduA"
                     /locus_tag="OO596_000010"
     CDS             7988..8272
                     /gene="pduA"
                     /locus_tag="OO596_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HCR6715991.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8269..9081
                     /gene="pduB"
                     /locus_tag="OO596_000011"
     CDS             8269..9081
                     /gene="pduB"
                     /locus_tag="OO596_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HCR6715992.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            9100..10764
                     /gene="pduC"
                     /locus_tag="OO596_000012"
     CDS             9100..10764
                     /gene="pduC"
                     /locus_tag="OO596_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HCR6715993.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10774..11442
                     /locus_tag="OO596_000013"
     CDS             10774..11442
                     /locus_tag="OO596_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HCR6715994.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11457..11975
                     /gene="pduE"
                     /locus_tag="OO596_000014"
     CDS             11457..11975
                     /gene="pduE"
                     /locus_tag="OO596_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HCR6715995.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11985..13817
                     /locus_tag="OO596_000015"
     CDS             11985..13817
                     /locus_tag="OO596_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HCR6715996.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13807..14157
                     /locus_tag="OO596_000016"
     CDS             13807..14157
                     /locus_tag="OO596_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HCR6715997.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14177..14452
                     /gene="pduJ"
                     /locus_tag="OO596_000017"
     CDS             14177..14452
                     /gene="pduJ"
                     /locus_tag="OO596_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HCR6715998.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14476..14898
                     /locus_tag="OO596_000018"
     CDS             14476..14898
                     /locus_tag="OO596_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCR6715999.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14901..15533
                     /locus_tag="OO596_000019"
     CDS             14901..15533
                     /locus_tag="OO596_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCR6716000.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15530..16021
                     /gene="pduM"
                     /locus_tag="OO596_000020"
     CDS             15530..16021
                     /gene="pduM"
                     /locus_tag="OO596_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HCR6716001.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16025..16300
                     /gene="pduN"
                     /locus_tag="OO596_000021"
     CDS             16025..16300
                     /gene="pduN"
                     /locus_tag="OO596_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HCR6716002.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16312..17319
                     /gene="pduO"
                     /locus_tag="OO596_000022"
     CDS             16312..17319
                     /gene="pduO"
                     /locus_tag="OO596_000022"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HCR6716003.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17316..18701
                     /gene="pduP"
                     /locus_tag="OO596_000023"
                     /pseudo
     CDS             17316..18701
                     /gene="pduP"
                     /locus_tag="OO596_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18712..19824
                     /locus_tag="OO596_000024"
     CDS             18712..19824
                     /locus_tag="OO596_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR6716004.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            19821..21166
                     /locus_tag="OO596_000025"
                     /pseudo
     CDS             19821..21166
                     /locus_tag="OO596_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            21169..21723
                     /gene="pduT"
                     /locus_tag="OO596_000026"
     CDS             21169..21723
                     /gene="pduT"
                     /locus_tag="OO596_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HCR6716005.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21723..22073
                     /gene="pduU"
                     /locus_tag="OO596_000027"
     CDS             21723..22073
                     /gene="pduU"
                     /locus_tag="OO596_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HCR6716006.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22078..22521
                     /locus_tag="OO596_000028"
     CDS             22078..22521
                     /locus_tag="OO596_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HCR6716007.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22622..22951)
                     /locus_tag="OO596_000029"
     CDS             complement(22622..22951)
                     /locus_tag="OO596_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6716008.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23123..24181)
                     /locus_tag="OO596_000030"
     CDS             complement(23123..24181)
                     /locus_tag="OO596_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6716009.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24379..24852)
                     /gene="sbmC"
                     /locus_tag="OO596_000031"
     CDS             complement(24379..24852)
                     /gene="sbmC"
                     /locus_tag="OO596_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCR6716010.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="OO596_000032"
     CDS             complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="OO596_000032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCR6716011.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26346..27773
                     /gene="sbcB"
                     /locus_tag="OO596_000033"
     CDS             26346..27773
                     /gene="sbcB"
                     /locus_tag="OO596_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6716012.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27816..28043)
                     /locus_tag="OO596_000034"
     CDS             complement(27816..28043)
                     /locus_tag="OO596_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6716013.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28057..29115)
                     /gene="yeeE"
                     /locus_tag="OO596_000035"
     CDS             complement(28057..29115)
                     /gene="yeeE"
                     /locus_tag="OO596_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6716014.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29294..30652)
                     /gene="plaP"
                     /locus_tag="OO596_000036"
     CDS             complement(29294..30652)
                     /gene="plaP"
                     /locus_tag="OO596_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6716015.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30642..30704)
                     /gene="yoeI"
                     /locus_tag="OO596_000037"
     CDS             complement(30642..30704)
                     /gene="yoeI"
                     /locus_tag="OO596_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6716016.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30919..31848)
                     /locus_tag="OO596_000038"
     CDS             complement(30919..31848)
                     /locus_tag="OO596_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6716017.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31894..32718)
                     /locus_tag="OO596_000039"
     CDS             complement(31894..32718)
                     /locus_tag="OO596_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6716018.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32801..33055)
                     /gene="yoeB"
                     /locus_tag="OO596_000040"
     CDS             complement(32801..33055)
                     /gene="yoeB"
                     /locus_tag="OO596_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6716019.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33052..33303)
                     /gene="yefM"
                     /locus_tag="OO596_000041"
     CDS             complement(33052..33303)
                     /gene="yefM"
                     /locus_tag="OO596_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6716020.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33586..33636
                     /gene="hisL"
                     /locus_tag="OO596_000042"
     CDS             33586..33636
                     /gene="hisL"
                     /locus_tag="OO596_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6716021.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33612..33735
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33782..34681
                     /gene="hisG"
                     /locus_tag="OO596_000043"
     CDS             33782..34681
                     /gene="hisG"
                     /locus_tag="OO596_000043"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6716022.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34687..35991
                     /gene="hisD"
                     /locus_tag="OO596_000044"
     CDS             34687..35991
                     /gene="hisD"
                     /locus_tag="OO596_000044"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6716023.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35988..37058
                     /gene="hisC"
                     /locus_tag="OO596_000045"
     CDS             35988..37058
                     /gene="hisC"
                     /locus_tag="OO596_000045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6716024.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37058..38125
                     /gene="hisB"
                     /locus_tag="OO596_000046"
     CDS             37058..38125
                     /gene="hisB"
                     /locus_tag="OO596_000046"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6716025.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38125..38715
                     /gene="hisH"
                     /locus_tag="OO596_000047"
     CDS             38125..38715
                     /gene="hisH"
                     /locus_tag="OO596_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6716026.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38715..39452
                     /gene="hisA"
                     /locus_tag="OO596_000048"
     CDS             38715..39452
                     /gene="hisA"
                     /locus_tag="OO596_000048"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6716027.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39434..40210
                     /gene="hisF"
                     /locus_tag="OO596_000049"
     CDS             39434..40210
                     /gene="hisF"
                     /locus_tag="OO596_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6716028.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40204..40815
                     /locus_tag="OO596_000050"
     CDS             40204..40815
                     /locus_tag="OO596_000050"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6716029.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40912..41889)
                     /gene="wzzB"
                     /locus_tag="OO596_000051"
     CDS             complement(40912..41889)
                     /gene="wzzB"
                     /locus_tag="OO596_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6716030.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42035..43201)
                     /gene="ugd"
                     /locus_tag="OO596_000052"
     CDS             complement(42035..43201)
                     /gene="ugd"
                     /locus_tag="OO596_000052"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR6716031.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43451..44857)
                     /gene="gndA"
                     /locus_tag="OO596_000053"
     CDS             complement(43451..44857)
                     /gene="gndA"
                     /locus_tag="OO596_000053"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6716032.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45021..46391)
                     /gene="cpsG"
                     /locus_tag="OO596_000054"
                     /gene_synonym="manB"
     CDS             complement(45021..46391)
                     /gene="cpsG"
                     /locus_tag="OO596_000054"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6716033.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46496..47932)
                     /gene="cpsB"
                     /locus_tag="OO596_000055"
     CDS             complement(46496..47932)
                     /gene="cpsB"
                     /locus_tag="OO596_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6716034.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47935..49158)
                     /gene="wcaI"
                     /locus_tag="OO596_000056"
     CDS             complement(47935..49158)
                     /gene="wcaI"
                     /locus_tag="OO596_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6716035.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49155..49634)
                     /gene="gmm"
                     /locus_tag="OO596_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49155..49634)
                     /gene="gmm"
                     /locus_tag="OO596_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6716036.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49637..50602)
                     /gene="fcl"
                     /locus_tag="OO596_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49637..50602)
                     /gene="fcl"
                     /locus_tag="OO596_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6716037.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50605..51726)
                     /gene="gmd"
                     /locus_tag="OO596_000059"
     CDS             complement(50605..51726)
                     /gene="gmd"
                     /locus_tag="OO596_000059"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6716038.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51752..52300)
                     /gene="wcaF"
                     /locus_tag="OO596_000060"
     CDS             complement(51752..52300)
                     /gene="wcaF"
                     /locus_tag="OO596_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6716039.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52316..53062)
                     /gene="wcaE"
                     /locus_tag="OO596_000061"
     CDS             complement(52316..53062)
                     /gene="wcaE"
                     /locus_tag="OO596_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6716040.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53073..54290)
                     /gene="wcaD"
                     /locus_tag="OO596_000062"
     CDS             complement(53073..54290)
                     /gene="wcaD"
                     /locus_tag="OO596_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6716041.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54265..55482)
                     /gene="wcaC"
                     /locus_tag="OO596_000063"
     CDS             complement(54265..55482)
                     /gene="wcaC"
                     /locus_tag="OO596_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6716042.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55479..55967)
                     /gene="wcaB"
                     /locus_tag="OO596_000064"
     CDS             complement(55479..55967)
                     /gene="wcaB"
                     /locus_tag="OO596_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6716043.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55970..56809)
                     /gene="wcaA"
                     /locus_tag="OO596_000065"
     CDS             complement(55970..56809)
                     /gene="wcaA"
                     /locus_tag="OO596_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR6716044.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56902..59064)
                     /gene="wzc"
                     /locus_tag="OO596_000066"
     CDS             complement(56902..59064)
                     /gene="wzc"
                     /locus_tag="OO596_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6716045.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59067..59510)
                     /gene="wzb"
                     /locus_tag="OO596_000067"
     CDS             complement(59067..59510)
                     /gene="wzb"
                     /locus_tag="OO596_000067"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6716046.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59516..60655)
                     /gene="wza"
                     /locus_tag="OO596_000068"
     CDS             complement(59516..60655)
                     /gene="wza"
                     /locus_tag="OO596_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6716047.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60964..61113
                     /locus_tag="OO596_000069"
     CDS             60964..61113
                     /locus_tag="OO596_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716048.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61314..62897
                     /locus_tag="OO596_000070"
     CDS             61314..62897
                     /locus_tag="OO596_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6716049.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63159..65024)
                     /gene="asmA"
                     /locus_tag="OO596_000071"
     CDS             complement(63159..65024)
                     /gene="asmA"
                     /locus_tag="OO596_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6716050.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKMLVTR"
     gene            complement(65046..65627)
                     /gene="dcd"
                     /locus_tag="OO596_000072"
     CDS             complement(65046..65627)
                     /gene="dcd"
                     /locus_tag="OO596_000072"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6716051.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65719..66360)
                     /gene="udk"
                     /locus_tag="OO596_000073"
     CDS             complement(65719..66360)
                     /gene="udk"
                     /locus_tag="OO596_000073"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6716052.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66678..69995
                     /locus_tag="OO596_000074"
     CDS             66678..69995
                     /locus_tag="OO596_000074"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6716053.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70105..70953)
                     /gene="alkA"
                     /locus_tag="OO596_000075"
     CDS             complement(70105..70953)
                     /gene="alkA"
                     /locus_tag="OO596_000075"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6716054.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71087..72439
                     /gene="yegD"
                     /locus_tag="OO596_000076"
     CDS             71087..72439
                     /gene="yegD"
                     /locus_tag="OO596_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6716055.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72452..74392)
                     /gene="yegI"
                     /locus_tag="OO596_000077"
     CDS             complement(72452..74392)
                     /gene="yegI"
                     /locus_tag="OO596_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR6716056.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74389..75150)
                     /gene="pphC"
                     /locus_tag="OO596_000078"
     CDS             complement(74389..75150)
                     /gene="pphC"
                     /locus_tag="OO596_000078"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCR6716057.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75147..75806)
                     /locus_tag="OO596_000079"
     CDS             complement(75147..75806)
                     /locus_tag="OO596_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6716058.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76027..76086)
                     /gene="ibsA"
                     /locus_tag="OO596_000080"
     CDS             complement(76027..76086)
                     /gene="ibsA"
                     /locus_tag="OO596_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR6716059.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76359..76415)
                     /locus_tag="OO596_000081"
     CDS             complement(76359..76415)
                     /locus_tag="OO596_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6716060.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76686..76742)
                     /locus_tag="OO596_000082"
     CDS             complement(76686..76742)
                     /locus_tag="OO596_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6716061.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77021..78262
                     /gene="mdtA"
                     /locus_tag="OO596_000083"
     CDS             77021..78262
                     /gene="mdtA"
                     /locus_tag="OO596_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR6716062.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78262..81384
                     /gene="mdtB"
                     /locus_tag="OO596_000084"
     CDS             78262..81384
                     /gene="mdtB"
                     /locus_tag="OO596_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR6716063.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81385..84462
                     /gene="mdtC"
                     /locus_tag="OO596_000085"
     CDS             81385..84462
                     /gene="mdtC"
                     /locus_tag="OO596_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6716064.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84463..85878
                     /locus_tag="OO596_000086"
     CDS             84463..85878
                     /locus_tag="OO596_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6716065.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85875..87278
                     /gene="baeS"
                     /locus_tag="OO596_000087"
     CDS             85875..87278
                     /gene="baeS"
                     /locus_tag="OO596_000087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6716066.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87275..87997
                     /gene="baeR"
                     /locus_tag="OO596_000088"
     CDS             87275..87997
                     /gene="baeR"
                     /locus_tag="OO596_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6716067.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21562 a        23316 c        22148 g        21053 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcctgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     6481 taatttattg ttatagcaaa acaattgttt tacatttaat cttcacatca tccctgatga
     6541 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
     6601 aataagttcg gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt
     6661 atttcgttat tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat
     6721 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
     6781 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca
     6841 ggtgaaaatc ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
     6901 ggtagaagca ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt
     6961 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
     7021 gaccacttcg accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa
     7081 gatactggcg agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa
     7141 atcggtaaac agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc
     7201 taattgcgcc agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
     7261 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
     7321 aagcgaaatc cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc
     7381 aactttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
     7441 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt
     7501 ttctttatca gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt
     7561 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
     7621 acaaattatt gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa
     7681 ttcccaccaa acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat
     7741 atcacagttc cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag
     7801 ccaacgcaat taattcacat tgaaatatcc acaaaacaac caataaatta acaacatata
     7861 gttcataaaa gcaattttat aatatttgtt ctctattaat ttctctcatt agccgattaa
     7921 gtcagccatt ctttttcctc gctttttttt atagtcatat cgtcaaaaaa atccaggcga
     7981 ggtctttatg caacaagaag cgttaggaat ggtagaaacc aaaggcttaa ctgccgccat
     8041 agaggccgca gacgccatgg tgaagtcggc aaatgttttg ctggtcggct acgaaaaaat
     8101 aggttccggg ctggtgaccg tcatcgttcg tggtgatgtc ggggcggtaa aagcggcaac
     8161 agacgcgggt gccgcagctg cgcataacgt cggcgaagta aaagccgtac acgttattcc
     8221 ccgcccacat accgatgtcg aaaaaatctt accaaaggga attaacccat gagcagcaat
     8281 gaactggtcg aacagatcat ggcgcaggtc atcgctcgcg tagcgacgcc agagcatgag
     8341 gttctcccga aaaccactca tcaaatacga gagacggcta tggcagaaaa aagctgcacg
     8401 ctaacagaat ttgttggcac cgctattggc gacactgtag gtctggtcat tgcgaacgtt
     8461 gatagcgccc tgttggacgc gatgaaactt gaaaagcgct atcgttccat tggcattctg
     8521 ggtgcgcgta ttggcgcagg gccacacatt atggccgctg atgaagcggt aaaagccacc
     8581 aacactgaag ttattagcat tgaacttcct cgcgatacta aaggcggtgc cggacacggc
     8641 tcactgatcc tcttaggtgg caacgatgtt tccgacgtga aacgcggtat tgaagtcgcc
     8701 ctgaaagaac tcgatcggac ctttggcgat gtctatgcca acgaagccgg acatcttgaa
     8761 ctgcaatata ccgcccgcgc cagctatgca ctggaaaaag cgtttggtgc accactggga
     8821 cgggcttgcg gcgttattgt cggcgctccg gctgctgtgg gtgttctgat ggccgacacc
     8881 gcgctgaagt ccgccaatgt ggatgttgtg gcatacagct ccccagctca agacaccagc
     8941 tacagcaatg aagtcattct gattatctcg ggtgactccg gcgcagtccg ccaggcggtc
     9001 atctccgcac gcgaaatcgg taaaaccgtg ctttcgactc tcggcgatac accgaaaaac
     9061 gatcaccctt cctacatctg aattctctga ggcagattta tgagatcgaa aagatttgaa
     9121 gcactggcaa aacgcccggt caaccaggac ggtttcgtta aagagtggat tgaagaaggc
     9181 tttattgcga tggaaagccc gaacgatcct aaaccgtcaa tcaaaattgt taacggcgtc
     9241 gttaccgaac tggatggaaa acccgccagc cagtttgacc tgatcgacca ctttatcgcc
     9301 cgctacggca tcaatctcgc gcgcgccgaa gaagtcatcg cgatggattc ggtaaaactt
     9361 gccaatatgt tgtgcgatcc aaatgtcaaa cgtagcgata tcgttcccct taccacagca
     9421 atgacgcctg caaaaatcgt cgaagtggtt tcgcagatga acgtggtaga gatgatgatg
     9481 gcgatgcaga aaatgcgcgc ccgtcgaaca ccatcacaac aggctcacgt caccaacgtc
     9541 aaagataacc cagtacaaat tgccgccgac gcagctgaag gcgcatggcg cgggtttgat
     9601 gaacaggaaa ccaccgttgc cgttgctcgc tatgcaccgt tcaacgccat cgccctgctg
     9661 gtcggctcac aggtgggacg tccgggcgtc ttgacccagt gttcactgga agaagccacc
     9721 gaactgaaac tcggtatgtt aggccacacc tgttacgccg aaacaatttc tgtctatggt
     9781 acagaacctg ttttcaccaa tggtgatgat accccgtggt caaaagggtt tctcgcgtca
     9841 tcctatgctt ctcgtggctt aaaaatgcgc tttacttccg gctccggctc tgaagtacag
     9901 atgggttacg cggaaggcaa atcaatgctt tacctcgagg ctcgttgcat ttacatcacc
     9961 aaagccgcag gcgttcaggg tctgcagaat ggttcggtaa gctgcatcgg tgttccatca
    10021 gctgtgccat caggcatccg cgcagtatta gcggaaaact taatttgctc atcgctggat
    10081 ctggaatgcg cctccagtaa tgaccagacc tttacccact cggatatgcg ccgtaccgca
    10141 cgtttcctga tgcaatttct gccaggcacc gactttatct cttccggttt ttccgctgtg
    10201 ccgaactacg acaacatgtt tgctggttca aacgaagatg ctgaagactt cgatgattac
    10261 aacgtcattc agcgtgacct gaaggttgat ggtgggctgc gaccagtacg cgaagaagat
    10321 gttatcgcta tccgcaataa agccgctcgc gctttacagg ctgtctttgc cggtatggga
    10381 ctaccgccta ttaccgatga agaagtcgaa gcggcaacct atgcccacgg ttcaaaagat
    10441 atgcctgagc gcaatattgt ggaagacatc aagttcgccc aggagattat caataaaaac
    10501 cggaacagcc tggaagtggt gaaagccttg gcgcaaggca gttttaccga tgtcgcccag
    10561 gacatgctca acatgcaaaa agcaaaactg accggcgatt atctccatac ctcagccatc
    10621 atcgtcgatg acggacaagt gctctctgcg gtcaatgacg tcaatgatta cgccggaccg
    10681 gctacaggtt accgcctgca aggggaacgc tgggaagaga tcaaaaatat tcccggtgca
    10741 cttgatccca acgaaattga ctaagggtga gaaatggaaa ttaacgaaaa attactgcgt
    10801 cagattatcg aagacgtgct ggcagaaatg caacccagcg acaagtccgt ttcatttcgc
    10861 gcacctgtct ctgcaactgt cccttccgcg ccagataccg gaaattttct gaccgaaatt
    10921 ggcgaagccc aacaaggcac tcagcaagat gaagtcatta ttgctgtagg cccggcgttc
    10981 ggcctggcgc aaaccgtcaa tatcatcggt attccgcata aaaacatctt gcgcgaagtg
    11041 attgccggta tcgaagaaga aggcattaaa gcccgtgtaa ttcgctgctt taaatcgtcc
    11101 gatgtcgcat tcgtagcagt cgaaggcgat cgtcttagtg gttcaggtat tgctatcggt
    11161 attcagtcga aaggcacaac ggttatccac cagcagggac ttcctccctt gtctaacctg
    11221 gaacttttcc cacaagcgcc tttgttgacg ctggatactt atcgtcagat tggcaaaaac
    11281 gccgcgcgtt atgcgaagcg cgagtctcca caaccggtgc cgaccctcaa cgatcaaatg
    11341 gcgcggccga aataccaggc caaatctgcc attttgcata ttaaagagac gaagtacgtg
    11401 gtcactggca aaaaaccgca agaactgcgc gtgacgtttt aacaaaggaa accttcatga
    11461 ataccgatgc aattgagtcg atggtcaggg acgtgttgaa ccgcatgaac agtttacagg
    11521 atgcagctcc tgtttctgcg gtccccaacg catcaatact gagcgccaaa gtgacggatt
    11581 acccgctggc aaataaacat ccggaatggg ttaaaacagc gaccaataaa acgctcgacg
    11641 atttcactct ggaaaatgtg ctaagcgata acgtcaccgc cctggatatg cgcattaccc
    11701 ctgaaacatt gcgtattcag gctgcgattg ccagagatgc tggtcgcgat cgtctggcga
    11761 tgaattttga acgcgccgct gagcttacct cagtaccaga tgatcgcatc ctcgaaatct
    11821 acaatgcgtt acgcccttat cgttcaacta agcaagaact tattgccatt gctgacgatc
    11881 ttgaacagcg ttatcaggcc aaaatttgtg ctgcatttgt ccgtgaagct gcggaacttt
    11941 atgtcgagcg caaaaaactg aaaggcgacg attaaggacg aactatgcga tatatagcag
    12001 gcattgatat cggcaactca tcaacagagg ttgcgctggc aacgctcagt gcaactggag
    12061 agttgtcgtt cgtgagtagc gccctggagg aaaccaccgg aatcaaaggt acgttgcgta
    12121 acgtccatgg tattcaggag gcgctcgcac aggcaacgaa aaaagtcggc atcaatgtta
    12181 gcgatatttc gcttattcgt atcaacgaag caactccggt cattggtgat gtggcgatgg
    12241 aaaccatcac tgaaactatc atcaccgaat caaccatgat cggtcacaac ccgaaaactc
    12301 cgggcggagt ggggctgggc gtagggttca ccatcactcc gcaagagtta ttaacacgcc
    12361 cggcggacac accttacatc ctggtggtgt catcggcatt tgattttgcc gatatcgcga
    12421 cgatgataaa cgcctccgta cgcgccggat atcagctgac tggcgttatt ctgcaacgcg
    12481 acgatggcgt actggtcaat aaccgcctgg aaattccttt acctattgtg gatgaagtcc
    12541 tctacatcga tcgtattccg ctcggcatgc tggcagcaat tgaagttgcc gttcccggga
    12601 aagttatcga aaccctgtct aacccgtatg ggattgccac cgtattcgcc ctcaatgctg
    12661 aagagactaa aaatattgtt cctgtcgcgc gggcgctaat cggcaaccgt tcagcagtgg
    12721 tagtgaaaac gccctctggc gacgttaaag cccgttcgat acccgccgga aatatcgaac
    12781 tgctttctgc cgggcgcaca acgcgcgttg atgtagctgc tggtgccgat gccattatga
    12841 aagcggtcgg tgagtgccca aaactggaaa acgtgacggg agaaccaggc accaacatcg
    12901 gcggaatgct ggaacatgtt cgccagacca tggcggaact gaccaacaaa cccagcaacg
    12961 aaatttttat tcaggatttg ctggcgatag atacctctgt ccccgtcagc gtaaccggtg
    13021 gtctggcagg tgaattttca ctggaacagg ctgtgggtat tgcctctatg gtgaaatccg
    13081 accgtctgca aatggcgatg atcgccagtg aaattaagca aaaactgcat gttgatgttc
    13141 aggtcggagg cgcagaagca gaagccgcca ttcagggggc cttgactacc cctggaacca
    13201 cacgtccact ggcaattctg gacttaggtg caggctctac cgatgcctcc atcatcaatc
    13261 agagtgggga aatcgttgcc actcaccttg cgggagcggg cgacatggta acgatgatta
    13321 tcgctcgtga gctgggctta aatgatcgtt atctggcaga agaaatcaaa aagtacccgt
    13381 tagcaaaagt agaaagcctg ttccacttac gccatgaaga cggtagcgtt cagttcttcc
    13441 ctactcccct atccccccat gtatttgccc gcgtctgtgt agtgaaaccc gacgaactgg
    13501 tgcctattcc cggtgatttg acgctggaaa aagtacgcgc tgttcgtcgc agtgcgaaag
    13561 agcgtgtctt cgtaaccaat gccctgcgcg cgctacgtca ggtaagccct gccggaaata
    13621 ttcgcgacat tccgttcgtc gttctggtgg gtggttcttc gctggatttc gaagtaccgc
    13681 aattggtcac cgacgcgctg gcacattatc gactggtcgc cgggcggggg aatatccgtg
    13741 gtagcgaagg tccgcgcaac gcggtagcta ctgggctact catcgcctgg cacaaggagt
    13801 cgattcatgg aaagtaaaga gcgtgcacca gccatcgttg tcatggagat cggtgactgt
    13861 atcactacat gggatgaggt gcttcttggc attgaagagg aaggtattcc ctttcgcatt
    13921 caacacattc cgtcaggtga agttatcgat agcgcctggc tggctgctcg tcaatctccc
    13981 ctgttggttg gcattgcctg cgaccgggaa gaactgattg tgcattacaa aaacttaccg
    14041 gcatcagcgc cactgtttac gctgacgtat caacaagata accatacccg gcgcagcatc
    14101 ggcactaacg ctgcccgcct ggtaaaagga attcccttcc gggaattcca ctcataatcc
    14161 acaggagaaa cgctgtatga acaacgcact aggactggtt gaaacaaaag ggttagttgg
    14221 cgcaattgaa gctgcagatg ccatggttaa atccgccaac gtgcagctgg ttggctatga
    14281 aaagattggt tcgggtctga ttacggtgat ggtccgcggt gatgttggcg cagtaaaggc
    14341 cgccgttgat gcggggagcg ctgcggcaag cgccgttggc gaagtgaaat cctgccatgt
    14401 catcccgcgc ccacacagtg atgttgaggc tattttacct aaatcggcat aaccccattt
    14461 gctaaggagc acagcgtgaa gcaatcactg ggattacttg aagccagtgg tctggctctc
    14521 gccatcagtt gcgcagacgc tatggcaaaa gccgcgtcca tcacgctggt tggccttgag
    14581 aagaccaatg gctcaggctg gacgctcatc aaaatcaccg gtgacgtcgc ctcggttaat
    14641 gcggcgatcg cgacgggagc cagtttcgcc ggacagcgaa atggccttgt cgcacacaaa
    14701 gtgattgccc gcccgggaga cggcattctg ccccacacaa ctgaagcatt gaccgtgacg
    14761 gttgaaacaa gcaaaacacc agcaacaacc gtcacctcag agattatcag ttgtaatttg
    14821 tgtcttgacc cggcatgtca gcggcaaaaa ggtgagcccc gctcactttg cctgtacgcc
    14881 aataagcgag gggagtagca gtggataaac aaaccttgca ggaaacagtg agccagattc
    14941 tggatgagat gcgcgagcgc cctatcccac tgggcgtttc cagtcgccat atacatcttt
    15001 catcagcgga ttatttgcgt ctttttcctg aacaaccgat tagcgagaaa aagccgttaa
    15061 tgcaacctgg ccaatacgct gctgaacagg ttgttacgct ggagggacca aaaggcaaat
    15121 tgcacaacgt tcgcttgttg gggccgctac gcaatgcaag ccaggtggaa atttcccgga
    15181 cggacgccag aacgttaggg atcaccgccc cgctacgcat gtcaggcgat ctcagcggca
    15241 ctccggggat tcgtttgata agcccatttg cggaactgga gatcacatct ggcgtcattg
    15301 tcgcgcaacg gcatattcat atgtctccgc tggatgccct gatttatggc gttacgcatg
    15361 gcgataaagt ggcggttgcc attgaaggtg atgagcgacg actgatattc gaaaacgtcg
    15421 ctgttcgcgt ctctccggat atgcgtcttg aaatgcatat tgataccgat gaggccaacg
    15481 ccgcaggcgc tgatagccct gacgtttttg ccacactggt gtcagtgcca tgaataacat
    15541 gctgatgcaa cgtattgttg acgaagtggt ttttcgcctt aaacaacgtg cgggaaaaac
    15601 actcgtgctg actgtttttc aattgcgaga cgccagcgtt caagagagcg ttcatcagta
    15661 tgcgtcatta caaatccgat atgtcgatct gccgctgctg cgacaacttg cggagaacga
    15721 aacgtcagat cgagcatcta tccagataca cgaagcgtta gcctggggaa tgcacattca
    15781 attgtcgttg cagcgtcatt tcctgaacgc catcgaattg aagactctcg cccgtctacc
    15841 gctaagctgg tgcgacgaac aagggcagcc gatctacctt catcataatc gacttttaag
    15901 ttatgccgat atcgcacagt taagctcggg aatactggtg ttacagcgga aatgttgtgt
    15961 gaccgcactg gctcgtgaag cggcaattac ccgaaatatt cagttaatca ggcaggagta
    16021 acctatgcat ctggctcgag tcactggcgc ggtagtatcc acgcaaaaat caccttctct
    16081 gatcgggaaa aaactgctgc tggttcgccg ggtcagtgct gacggcgaac tgcctctcac
    16141 gccattaacc ggcgacgaag ttgccgttga ttcagtcggc gcaggcgttg gtgagttggt
    16201 actcctcagc agtggttcca gcgccagaca tgtgttttca ggccctaacg aggccatcga
    16261 cctggcagtg gtcggcattg tcgatacgct ttcgcgctaa ggagctaaaa gatggcaatt
    16321 tatacccgaa caggtgattc aggaagtacg tctttattta ccggacagcg cgtcagcaaa
    16381 actcatctac gtgtcgaaac ctatggcacg ctggatgaac tgaacgcgac actcagtctg
    16441 tgttattgcg ccaccgccat cgaaagccat cgcatcttac tggaagccat tcaacaacag
    16501 atattttggt ttagcgccga actcgccagt gaaagcgaac agccgtcagc gcaacagcgt
    16561 tacatcggta cggaagaaat tgccgcgttg gaaaacgcga tcgatagtgc aatgaacgcc
    16621 gtccccccag ttcacagctt cattctgcct ggtcgatgtg aagccgcaag ccgcatgcat
    16681 ttcgcccgca cggtggctcg acgggctgaa agacggctgg ttgaactgac aacggaaacc
    16741 accgtccgga atgttttgct gcactatatc aaccgacttt ccgattgcct gtatgccctc
    16801 gcccgcgtgg aagacaacgt tgctcatcaa aacctgatga ttcaggaaat cacaaaacgt
    16861 tatcacgaag ccaaccacac accagcattg aaggaacgca caatgccgct cacttttcag
    16921 gatcttcacc agctcattcg tagtgcggca atgcgcgcgg atgaactgca tattcccgtt
    16981 gtcatcagca ttgttgatgc caacggaaca gaaagtgtta cctggcggat gcctgatgcc
    17041 ctgttggtca gcagtgaact ggcaccgaaa aaagcctgga ccgccgtggc gatgaaaacg
    17101 gcaacccaca agcttgctga taccgtgcaa cccgacgcgc cgctttacgg gctcgaaagc
    17161 catatgcaag gaaaagtcgt cacttttggt ggcggcttcc ccctctggcg tgacggaaaa
    17221 ttgcttggcg ggcttggcat cagcggcggt agcgttgaac aagacatgga tattgcccaa
    17281 agcgcaatgg cggcaattaa cgtgggagta aaccaatgaa taactctgaa ttagaaacac
    17341 tgatccgcaa tattctgcgt gagcaacttg tccccgctac accacgaacg caaagaaacg
    17401 ctattttcca gactgtcgat gaagcggtct gcgcagcaca ctaggccttt ttacgctttc
    17461 aacagtgccc gcttaaaacc cgtagcgcca ttatcagcgc cattcgcgaa gaattaatgc
    17521 cccgccttac cgaactggca gaagaaagcg ccaaagaaac ggggatggga aataaagaag
    17581 ataagtacct aaaaaacaaa gcggcactcg ataacacgcc aggaattgaa gatttaacca
    17641 ccacagcact aaccggtgat ggtggcatgg ttttgtttga gtactctccg tttggggtga
    17701 ttggctcggt cacgccaagc accaacccca cggaaaccat cattaacaac agcatcagca
    17761 tgctggcagc aggtaatagc gtctacttca gtccacaccc gggtgccaaa aacgtttcgt
    17821 taaaactgat taccacaatc gaagacatcg cgtttcgctg tagtggcatt cataacttga
    17881 tcgttaccgt atctgaaccg acctttgctg ccaccgaaca gatgatggcg caccctaaaa
    17941 tcagcgttct ggcgattacc ggcggtccgg ggatcgtcgc catgggaatg aaaagtggca
    18001 agaaagtgat tggtgccgga gcgggtaacc ctccgtgcat cgtcgatgaa acggcggata
    18061 ttgtgaaagc cgccgaggac atcatcaacg gcgcttcatt tgattacaac ctgccgtgta
    18121 ttgctgaaaa aagcctgatt gtcgtggcat cagtggcaga ttatttaatt cagcagatgc
    18181 aaagttttgg cgcacttctt cttaactatg agcaaactga gaaattgcgt gcaatttgcc
    18241 tgccagacgg tagcgccaac aaaaaactgg ttgggaaaag cccatcagcc ttgctggaag
    18301 cggcaggcct ccctttgccc gcaaaagcgc cgcgcctact gattgctgtt gtcgacgccg
    18361 atgatagttg ggttacttgt gagcaactaa tgccaatgct gcccattgtg aaggtcaagg
    18421 attttgacag cgctctaacc ctggcgctga aagtggaaga cggcttgcac cacacggcaa
    18481 ttatgcactc tcagaacgtc tcacgactca accttgccgc gcgcgttatg caaacctcaa
    18541 tatttgtcaa aaacggcccc tcctgggcgg gtattggcgt cggcggcgaa ggctttacga
    18601 cctttaccat tgcaacaccg actggagagg gtacaacgtc agcacggact tttgcccgct
    18661 cacgacgctg cgtgttaacc agcggctttt caattcgcta gagaaacgga aatgaatact
    18721 ttttcactgc agacgcgttt atacagcgga ccaggaagtc tcaaggtact tagccgtttc
    18781 tctcatagac acatctggat tatttgcgat gcctttctgg cacgctcacc acttattgac
    18841 acgttgcatc aggcgctgcc cgacagtaat cgtctcagta tttttagcga aataacgccc
    18901 gccccaacta tccagaccgt ggtgaaaggc attgcgcaaa tgcagacttt acgcccggat
    18961 gtagttatcg gttttggcgg cggttctgcc ctggacgccg caaaagcaat tgtctggttc
    19021 ggtcgccagt gtggcattga aattgaaacc tgcgtagcca ttcctaccac cagcggtatc
    19081 ggttccgaag tgaccagtgc ttgtgtcatc agcgatcctg agaaagggat taaatatccc
    19141 cttttcgata atgcacttta tccggatatt gcgatccttg atccatcttt ggtcgtcagc
    19201 gtgccaccgg cgataacggc caataccggg atggatgttt taacccatgc gcttgaagct
    19261 tatgtttctc ctcgcgccag cgacttcacg gatgcactgg tcgaaaaagc cgtgcaaatt
    19321 gtttttcaat acctgcccac tgcagtgaaa aaaggcgatt gtcttgccac ccgtggcaaa
    19381 atgcataatg catcgacgct tgcaggaatg gcctttagcc aggccggact cggtttaaac
    19441 catgcaattg ctcatcaact cggcggacag tttcatctgc cgcatggact ggcgaatgcg
    19501 ctcctgctca cggcagttat ccgctttaat gctggcgatc cgcgcacagc caaacgctat
    19561 gcccggctgg cgaaaacgtg ccatttatgc ccggacaacg ccaatgacac cgccagcctg
    19621 aatgcactta ttcaacatat tgagcagctt aatacaacct gtacgctgcc gactcttgcc
    19681 aatgcgctga aagaaaaaaa agcagaatgg tcaatacgca taccagatat ggttcaggcg
    19741 gcgctggcgg atgcaacgtt gcgtaccaac ccgcgtgcag ctgatgcctc cgcgattgca
    19801 gaactgctca aggggttgtt atgaacgtcg gattaatctc ctgcgacgac cagaccatcc
    19861 gcgagcgagt acaagcagca ggtgtcgtgg gtgctggcgg cgcaggtttt ccaacccata
    19921 ttaagctaca agcgcgtgct gacactttcc tggtgaatgc ggcggaatgc gaacccatgt
    19981 tgaaagtaga tcaacagtta atggcgcagc aagccagccg cctgcttcgc ggtgttcact
    20041 atgcgatgaa agccaccggg gccagtagcg gaatcatcgc gctgaaagaa aaatatcaac
    20101 gggcaataaa cgcactcaca ccactgttac caccggatat ccgcctgcat attttgcccg
    20161 acgtttaccc cgcaggagac gaagtgctga ccatctggat ggcaactggt cgccgcgttc
    20221 cacccgctgc attacccgtt agcgtcggtg tggtggtcaa taatgttcaa accgtactta
    20281 atattgccag agccgtcgag caacagtatc cagtgacgcg ccgcacactc accgttaatg
    20341 gtgcagtggc gaagccaata acggtaaccg tccccatcgg catgtcttta cgtgaagtcc
    20401 tggcgctggc gggcggcgca acagtggatg ctcctggttt tatcaatggc gggccgatga
    20461 tgggaagttt gatcacatcg ctcgacacac cagtcagcaa aaccactggc ggcttgctgg
    20521 ttttacccaa ctctcattca ctgatccaac gtcgaatgca gaatgaccgc agcgtacttg
    20581 ccgtcgcgaa aactgtttgt gagcaatgcc gcttgtgtac cgatctctgc ccacgtcatt
    20641 taatcggtca cgaactggca ccccatttat tagtacgtgc gcggttaact accaacagct
    20701 cgccacgccg caactgttac tgacagcgct cacctgttca gagtgcaacg tctgcgccag
    20761 tgtcgcctgt ccggtaggaa tttcaccaat gcgcataaac cgcctgctta agcaggaatt
    20821 acgcgcacaa aatctccgct acgaaggtgc cttaaatcct gcagatccca tggcaaatta
    20881 tcgactgatt cccgtaaaac ggctggtaac cagacttggc ttaaccccgt ggtatcagga
    20941 tgcgccattg tctgaacagg tgcctcagcc cgaaaaagtg acgttattgt tacgccagca
    21001 catcggtgcc agcgcaattg cgtgtgtaca aaaaggcgat cgcgtcgttc atgggcaatg
    21061 tgtaggtcaa attccacacg gcacattagg tgcccccatt catgccagta ttgatggcat
    21121 ggtcagcgat gtgacagaaa acgcgattac gcttgtgaga ggttaaccat gtcgaaagcg
    21181 gtaggtattt tagaacttag cagtatcgcg aaagggatgg aaaccggcga cgccatgctg
    21241 aaaagcgcca acgttgagtt actggtcagc aaaacgctat gccccggaaa atttctgctg
    21301 atgctcggtg gcgatgtggg cgcggtgcaa caggccatca acagcggagc gtcccttgct
    21361 ggtgaaatgc tggtcgacag tcttgtcctg ccgaatattc atcccagcct cctttctgca
    21421 atcagcggac ttaaccatgt tgaacagcac cgggctgtcg gcgtagtcga aacctggagt
    21481 gtcgccgcct gcattgacgc tgccgattgt gctgtcaaag ctgcaaatgt cattcttgtt
    21541 cgcgtacata tggcttttgg cattggcggt aaatgctaca tggtggttgc tggcgatatt
    21601 tcagatgtta ataacgcggt ctctgccgcc agcgtgcgcg cgggggaaaa aggtttgttg
    21661 gtctatcggt cggtgattgc ccgtccacat gaagccatgt ggcgtcagat ggtggaggga
    21721 taatgacaac tcagcacaca actgaacgga tgattcagga atatgtccca ggtaagcagg
    21781 tcacgctggc acatgtcatc gccaatccag ggaaggatct gtttaagaaa ttgggtttac
    21841 cagacaccat ttccgctatt ggtattctga caattacccc cagcgaagca tctatcatcg
    21901 catgcgatat cgccacaaaa tcaggcgctg tagagatcgg ttttcttgat cgttttaccg
    21961 gtgccgtagt gctgactggt gatgtttctg cgatcgaata tgcacttagc caggttaccc
    22021 gtacgctggg tgaaatgatg cgctttaccg cctgccccat tacgcggacc tgacgttatg
    22081 caacgtatga tgctcatcgg ccccagccag tgcggaaaaa cgtcactcac gcaatgtctg
    22141 cgtggcgaaa atattcatta tcagaaaacg caggcaatcg tctggtcccc gaccaccatt
    22201 gatacaccag gcgaatacct cgaaaaccgc agcctctaca gtgcactgtt ggtcagtgcc
    22261 tgcgaagcgg acattatcgc gctggtgctt aatgcggacg caccgtggtc acctttttcg
    22321 ccaggcttta cctcgtcaat gaatcgcccg gtcattggag tagtaaccaa agcagattta
    22381 gccaacatgg aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac
    22441 gtgttagtaa ccagtgcggt taataaccat ggagttgcag aacttttagc cttgctgcat
    22501 acagaagaag gctgtcgcta atttcgacag gttatcagat gggaatgatg acggtgatac
    22561 acagaaaaga atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga
    22621 attacttcgc ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc
    22681 ggcgttcttt ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt
    22741 cacctttcat gctggcgatg atgccctgga ttgcttcaac gctcatccca ggcttgatgt
    22801 aatcgctcag gttgtccagc agcaggacgc gcttctggtt gtcacggatc tttttctcaa
    22861 cgtcctgaat ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta
    22921 cgtcctggaa tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg
    22981 attcgtttaa gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac
    23041 aatgctttta tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca
    23101 agatgattct gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca
    23161 actcaagctg atgcgccgtt tccatgttca gccacacata accatagatt ggtgtttcca
    23221 ctaccttcag gtcatggtgg ttattgagca actgacgcag ctcttccaca gcatcgttca
    23281 atttttccgt attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaagc
    23341 tcagcagtat ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca
    23401 cgaaatggct ggggcgcgtg gcccagtacg cattgatttg caactccagc atacaaacca
    23461 gattgcggtt aatggtctgg ataccttcat atatcgattt tggaatacgg gtttctttgc
    23521 tggcgggcgc aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc
    23581 tttccagacg tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat
    23641 actcggtcag acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga
    23701 taccggtaaa caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg
    23761 ccgtatcaat ttcacctgtc ggggaaccaa caacaattgc cagcgtcacc ccaatcaata
    23821 atccttgata cggtttcttg cccagcgcca gccaaccgca aagaaacatc gccgccgcgc
    23881 accagactaa catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa
    23941 tcgaacccaa caccgtaccg ccaatacgtt cgaaggcacg agggacaacg ttaccccaga
    24001 acgagattgg ccccataatc accaccatgg tgaccagcgg ccaggtgctt tccgggatag
    24061 taaacaggcg gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca
    24121 ctatgcggta atggcggtat acccggattt caaacgggct taatgactta tcggcacgca
    24181 cgcagaaact ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg
    24241 cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa
    24301 aaattgaaag gcgcatctta ctcttttcgc tttcaaaaaa agagtggtca tcgcgttaac
    24361 acaccgccct gagatgaatt agtgatgttt tggctgcacc gcaacataca tttcgatatc
    24421 ccagtaccca tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat
    24481 ttcataagca ctgtcctgca agaggctatt aaagaactga taccagggtt tagcaaaatc
    24541 atcaccgact acacgagcca ccgccaccgc atactgacca cctgaaattt ctgtcagaat
    24601 gacgccctca ctgttttcgg gaagcgtaaa gttattcggt accgtcacaa cggtgtcgca
    24661 gcgtaatttt tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt
    24721 cggcacaata tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt
    24781 ctgttcccac gggccaacga gatggaaacc tgcaacggta cgtttctctt cctgcgtaat
    24841 ctcgtagttc atgacgcctc cattaacact gtttttatat acagcatagt tgcaatatta
    24901 aaaccacaag gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc
    24961 aaaagaaagg tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt
    25021 ccccgacaga ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa
    25081 gttcaatttc ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa
    25141 gggtatattt ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt
    25201 gttccgttcc taggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct
    25261 ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt
    25321 ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac
    25381 gacgctgccc atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg
    25441 ttttcaggcc gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg
    25501 tgataccgtt ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt
    25561 ggatgatagc gcgagaaaac acagctaaat cataagccga gctatgctgg ccaggtgcat
    25621 ccagaccatg cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt
    25681 tcatcatttc aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag
    25741 cgtcatttcc ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc
    25801 cctctttcaa aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat
    25861 cgcgccccac ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca
    25921 cgacataacc cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac
    25981 ccgcggtgag gatctgaccg gtggtgtaat ccatcaatac ccaggacccg gcatgaatct
    26041 ctggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa
    26101 cgaacaaaga agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa
    26161 atagctgttc tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt
    26221 gactaccctc tcatttttat ctgacatgat ctgttgccac tcgctgccaa attgtggcgc
    26281 taaagctgat tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta
    26341 acctaatgat gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct
    26401 ttggcacgca ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgataacg
    26461 aattcaatgt catcggcgaa cccgaagtct tttactgcaa gcccgctgat gactatttac
    26521 cccagccagg agccgtatta attaccggta ttaccccgca ggaagcacgg gcgaaaggag
    26581 aaaacgaagc cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta
    26641 ttctgggcta caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta
    26701 atttctacga tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttgctgg
    26761 atgttatgcg tgcctgttat gccctgcgcc cggaaggaat aaactggcct gaaaatgatg
    26821 acggtctacc gagctttcgc cttgagcatt taaccaaagc gaatggaatt gaacatagca
    26881 acgcccacga tgcgatggct gatgtgtacg ccactattgc gatggcgaaa ctggtaaaaa
    26941 cgcgtcagcc acgtctgttt gattatctct ttacccatcg taataaacac aaactgatgg
    27001 cgttgattga tgttccgcag atgaaacccc tggttcacgt ttccggaatg tttggggcat
    27061 ggcgcggcaa taccagctgg gtggcaccgc tggcgtggca tccagaaaat cgcaatgccg
    27121 taattatggt ggatttggca ggagacattt cgccattact ggaactggat agcgacacat
    27181 tgcgcgagcg tttatatact gcaaaagccg atcttggcga taacgccgcc gttccggtta
    27241 agctggtgca tatcaataaa tgtccggtgc tggcccaggc gaatacgcta cgcccggaag
    27301 atgccgaccg actgggaatt aatcgtcagc attgcctcga taacctgaaa attctgcgtg
    27361 aaaatccgca agtgcgcgaa aaagtggtgg cgatattcgc ggaagccgaa ccgtttacgc
    27421 cttcagataa cgtggatgca cagctttata acggcttttt cagtgacgca gatcgtgcag
    27481 caatgaaaat tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg
    27541 tcgataaacg gattgaaaag ctgttgttca attatcgggc acgcaacttc ccggggacgc
    27601 tggattatgc cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgcccgagt
    27661 ttttacaagg ttatgcagat gaattgcaga tgctggcaca acaatatgcc gataacaaag
    27721 agaaagtggc gctgttaaaa gcgctttggc agtacgcgga agagatcgtc taatttgaag
    27781 cctccgccgc tggtacggcg gaggtcaatg ttgagtcagg ctttttgaac ggtgatactc
    27841 cacgctgcat cgccaatctg ctgataatcg gtgatggcat gcccttcttc tgccgcccac
    27901 tgtgggattg cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg
    27961 gccatttctg ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc
    28021 acatccagct ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag
    28081 tcgcgcctta cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat
    28141 cataaataca aggccaatcc agccctgcca ggtcatcatt gcagtcataa ccagtccatt
    28201 accgataaag caaccacctg caatactggc accgaagccc atcagtacac cacctaaccc
    28261 gctacgtaat gttgtttgtg catcagctgc gcgaacgcgg aactcacggc tcgctttggc
    28321 tgcaataaat gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt
    28381 cacgtcaccc gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc
    28441 aaacatgcgt ccggttgctt cactcagggg ccaggctaaa agcgcaatca aaccgatgag
    28501 tacagcagtg acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccagttcg
    28561 gcgcggcggt aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca
    28621 gagcgttatc accagcaaaa cggcaaccaa cggccaaaca gacaaattaa aagtctcagc
    28681 aatagagtta tgttcagtac tgtagtgctg caaggtttga tttaaaccac tggcatgtgg
    28741 agaacgcatc accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa
    28801 tccctcaccc gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag
    28861 cccgaagata tacccaccta taacagtacc aagccacggg aacgcctcgg cttcgtaagt
    28921 cagtaggccc gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag
    28981 caaggcgtaa aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag
    29041 gcaaaaacgc ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa
    29101 tatcattgaa aacataaatt aattaaccag atgaatgtta atgaggaaat tattcatgac
    29161 tggtggaatg cagaccaata accaaattct ctaaattaga acaaatggtt attaatgagg
    29221 atttcgataa aaaaaaataa aaaaaccgga gccaaatatt cattcgactc cggttgatta
    29281 ttagaaaaga tgattatgca acgtcttcgt actgcggaac tgggttacgg aagctcttgg
    29341 tgacgcaagc caggtaaatc agaccgatag cagcccaaat cagacccaga accattgagc
    29401 tttcttccag gttaacccac agcgcaccaa cggtcagcgc accacacatc ggcaggaaca
    29461 gatactggaa gtgatctttc agcgtcttat tacgcttctc acggatccag aactgcgaga
    29521 tgaccgacag gttaacgaag gtgaacgcca ccaacgcacc aaagttaatc agcgctgtag
    29581 ccattaccag gtcgaagttg attgccagca gggcaatcgc gccaaccagg atgatgttca
    29641 tcgccggagt acgccatttc gggtgtacat aaccgaagaa gcttttcggg aatacgccgt
    29701 cacgacccat tacgtacatc agacgcgcta cgcctgcatg cgccgccata ccggacgcca
    29761 gtacggtaat ggtggagaag atcagcgcgc caacctggaa cgctttaccc gcaacgtaca
    29821 gcatgatttc aggctgtgac gcatccggat ctttaaagcg agagatatcc gggaagtaca
    29881 gctgcaggaa gtaagttgca aagatgaaga tcatgccgcc aatcagcgcg gtcaggaaaa
    29941 tcgcacgcgg gatcacgcgc tctgcatctt tggtttcttc cgacaggttg ctgatgccgt
    30001 caaagccggt aaaggaaaag cacaggattg tcgccccggt aatcatcgga ataacatgcg
    30061 catcgccaga ccagaacgga cgagtgctcg ccagcgtacc agcgccttca ccttcaaata
    30121 cgccataaac aaccatgccc agaatcaccg cgatcagcac tacctgcaac acgacgatta
    30181 cagtgttgaa gttcgctacg gatttcagac tacgcaggtt aaaggcggtc ataaaggcca
    30241 ccagcgccac cacaaacatc cacgatggga tggaaggcac cagagcttca aaatagattt
    30301 tcgccagcag aatgttgatc atcggcgcga acagatagtc gagcagagaa gaccaaccca
    30361 ccataaagcc gacagtcggg ctaatggatt tctgggcgta agtatatgca gagccagcag
    30421 aaggatagcg gcgaaccagc ttcccgtagc tcagagccgt aaacaggatc gcaatcaatg
    30481 cgaacgcata ggctgtcggc acatgaccat ccgtgaggcc tgaaacgata ccaaaagtat
    30541 caaacagcgt catcggctgc atataggcaa gacccatcat tacaaccgga actaacgtaa
    30601 gcgttttacg caattccacg cgagaggtgt ttggagtaac gttatgcgac atggtcattc
    30661 tcctttacgg tgataaccgt cgcgtaagca aaaaattgcc ccattttttt ggattcctca
    30721 gcgacaacaa ctgtcgattt ttagtaaata tctatccggt acgaagcccg gcctcttggt
    30781 atgaatgatt ggtttgaagc aaaaaataac cgacgctgat gaaacgtcgg tttttagtca
    30841 ttttttgaca gcggcgcatt gtgcctaaat gggggggaaa tgacaagaga atgagaagct
    30901 tgtcataata atttttcttt aaatggctga ttttccgtca tcagattttg cataaacacc
    30961 ggcgatcgca ctatttgcta aaatttcatc ccgccaccag ccgctggcaa gccctgcggt
    31021 ctgttcgttc cagccaatcc agacgggttc aaagagagtt tgggcgacca ctttcttctc
    31081 gattaacgcg ccactatcga gaaaacgttg caccagataa cggggtaaat agccgcagcc
    31141 gaggccgcta atttgcagct ccagcttggt tttaaaatcg aaaacggtaa tggcttcttg
    31201 ttcgtcgaga agttgtgagg ccgtagaagc gcctgcctgc gcagtgtccc ccaccacaat
    31261 cgcccgatat cgcttaatga tacgacggtt taacggctct tcttcctggg ctaacggatg
    31321 atgaggcgcg accgcaaaga cttgttccag atcgcccaac cgcgagaaac caaactcact
    31381 ggaagatggt ggctcatgca tcgcgccaac aataatatcc gctcgcccct gagtcagggc
    31441 atcccaggaa ccaccgagta cgccattgat aaacttcagg cgcgtcacgc tatgatgttg
    31501 ataaaaagct tcaatcagtg gcgcaagcag agaaaaagga aacgtgtcgt ccacgccaat
    31561 caccagctcg ttttcccagc cttcatgcag tttgatcgcc tgcttttcca gttctcgcac
    31621 ggtatgcaga acttcccgcc ccttctctaa taacattttt ccggtacggg tgaatttagc
    31681 gcggtgaccg ctacgatcaa gtaattgaat attaaggtcg ctttccagct tgtgaacggt
    31741 gtaactcaga gcggacggcg tcttgtagag tttagccgac gccgccgcaa aactgccttc
    31801 tttttctaag gcatcgagga tcatcaacac atccagcagt tgtttcatac acgccccctt
    31861 gcagtgcatg cggcgtcctg ctggcgtgat cgtttactcc agcggcatta ccagcggatc
    31921 gggatactgg tattcaaatc ccagttcatt acaaatccga ctgccatcaa taatcttacc
    31981 tttgccgctg tccagactat ttctgaactg cggaggttcc agccccagta aacgggccat
    32041 ctgcggatag aaaacattac gcgcagggtg agctggcgca catatattat agatgtgtcc
    32101 gcctttaggt gcctgtaaca acagagtgat agcgccaata acatcttcta aatggactaa
    32161 attaacacca tgttcaccat caggcgcggt ttttccggca aagaagcgtc cgggatgacg
    32221 tcccggtccg accagccccg caagacgcag aatatcgacc gaagtaccgg gtaaattgtg
    32281 cagccagtct tcgagttctt ctaatactcg tccactgttg gtcaccggat tacgcggggt
    32341 ggtttctttc accgtgcctt gcgcgtcgcc atagacagac gttgagctgg taaaaataat
    32401 gcgaggaata cgatgggcca gcgcgctatc cactaactct tgtaccgctt gtaaatagaa
    32461 ctcatcgccg gggccgctac gacgtgccgg aagcgtaatg accagcgcat cggcatccat
    32521 cagggcatcc agatcgtcag aatcgcaaac taactcaggc tccatgcgaa gcagatagct
    32581 atcaatgcca ctcattcggg ccgcttcgac accatcttgt gtggttttac tcccggtgac
    32641 ttgccagcct cgcgctgaaa gtgacatcgc cagcggcatg cctaaccacc ctaacccgac
    32701 aattgcgacc tttttcatcc gttatctcct gatacacctt agatctataa ggctacgcta
    32761 gcgtatcaaa actgacaatt cattctatga atgaatctgt tcaataatga taacgacatg
    32821 ctgcaatgag cagtgaatcg tcggtaaccg cgtataccag acggtgctcc tctgtaatgc
    32881 gtcgggacca gaaacctgac aaattatgtt tcaggggttc tggcttcccc ttaccttcaa
    32941 atggcgttct gcgggtatct ttgataagtt cattgatctt tttaacaatt cgcttatctg
    33001 tttcctgcca gtacagataa tcatcccatg attcctcaga ccagattagt ttcactcaat
    33061 aatgtccttt tctgttcctt tgcctgattt caggctatcg attgagtcca tcaatctccg
    33121 ggcgttagcg ggggagcgca gtagataagc cgtctcttcc agcgagttgt attcttcgag
    33181 tgacatcaga acacaagcct ctccattctg acgagtaata aggatcgggg catgatcttc
    33241 aacggctttc atcattgttg ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg
    33301 catgtcaatc tcctcttttg tacagtttat tgtacaatga tgggcgttaa ttaactattt
    33361 attaattagt ttgtagatca aggtattgtc agtgagacga aaatccaggc ttcgctattt
    33421 ttggtgccgt cagcttagag gacagtcctc ttagccccct cctttccccg ctcattcatt
    33481 aaacaaatcc attgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt
    33541 ttaggttaaa agacatcagt tgaataaaca ttcacagaga cttttatgac acgcgttcaa
    33601 tttaaacacc accatcatca ccatcatcct gactagtctt tcaggcgatg tgtgctggaa
    33661 gacattcaga tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc
    33721 cgggggcttt tttattgcgc ggttgataac ggttcagaca ggtttaaaga ggaataacaa
    33781 aatgacagac aacactcgtt tacgcatagc tatgcagaaa tccggccgtt taagtgatga
    33841 ctcacgcgaa ttactggcgc gctgtggcat taaaattaat cttcacaccc agcgcctgat
    33901 cgcgatggca gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg acattcccgg
    33961 tctggtaatg gatggcgtgg tagaccttgg gattatcggc gaaaacgtgc tggaagaaga
    34021 gctgcttaac cgccgcgccc agggtgaaga tccgcgctac tttaccttgc gtcgtctgga
    34081 tttcggcggc tgccgccttt cgctggcaac gccggttgat gaagcctggg acggcccgct
    34141 ctccttaaac ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc gttatctcga
    34201 ccagaaaggc atctctttta aatcctgctt actgaacggt tctgttgaag tcgccccgcg
    34261 tgccggactg gcggatgcga tttgcgatct ggtttccacc ggtgccacgc tggaagcaaa
    34321 cggcctgcgc gaagtcgaag ttatctaccg ctcgaaagcc tgccttatcc agcgcgatgg
    34381 tgaaatggaa gaatccaaac agcagctgat cgacaaactg ctgacccgta ttcagggtgt
    34441 gatccaggcg cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac gtctggatga
    34501 agtcatcgcc ctgctgccag gtgccgaacg cccaaccatt ctgccgctgg cgggtgacca
    34561 acaacgcgta gcgatgcaca tggtcagtag cgaaaccctg ttctgggaaa cgatggaaaa
    34621 actgaaagcg ctgggtgcca gttcaattct ggtcctgccg attgagaaga tgatggagtg
    34681 atcgccatga gctttaacac aatcattgac tggaatagct gtactgcgga gcaacaacgc
    34741 cagctgttaa tgcgcccggc gatctccgct tctgaaagca ttacccgcac tgttaacgat
    34801 attctcgata acgtgaaagc acgcggtgat gacgccctgc gggaatacag cgcgaagttt
    34861 gataaaacca cggttaacgc gctgaaggtg tctgctgagg agattgccgc cgctagcgaa
    34921 cgcctgagcg acgagctaaa acaggcgatg gcggtggcag taaaaaatat tgaaaccttc
    34981 cacactgcgc aaaaactgcc gccggtagat gtagaaacgc agccaggcgt gcgttgccag
    35041 caggtcacgc gcccggtcgc ttcagttggg ttgtatattc ctggcggctc cgccccgctc
    35101 ttctcaacgg tattaatgct ggcgactccg gcgcgtattg cgggctgtaa aaaagtggtg
    35161 ctgtgctcac cgccgccgat tgccgatgag atcctttatg cggcgcagct gtgcggtgtg
    35221 aaggacgtgt ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt tggtacggaa
    35281 tctgtgccga aagtggacaa aatcttcggg ccgggtaacg cctttgtcac cgaagcgaaa
    35341 cgtcaggtga gccagcgtct ggacggtgcg gctatcgata tgcccgcagg cccgtcggaa
    35401 gtgttggtca ttgccgacag cggcgcaacg ccggatttcg tggcttctga tttgctttct
    35461 caggctgaac acggcccgga ctcacaggtg attttactga cgcctgacgc tgatatggcg
    35521 catcaagttg ccgaagccgt cgaacgccag ttagcagaac tgccgcgtgc cgaaaccgcc
    35581 cgccaggcac tgaacgccag ccgcctgatc gtgactaaag atttagcgca gtgcgtagag
    35641 atctccaacc agtacggccc ggagcacctg atcatccaga cccgcaacgc ccgcgatctg
    35701 gtcgatggca tcaccagcgc cggttcggta tttcttggtg actggtcacc ggaatcggca
    35761 ggtgattacg cctccggcac caaccacgtt ctgccgactt acggttacac cgccacctgt
    35821 tccagcctcg ggctggcaga tttccagaag cgtatgaccg tgcaggaact gtcgaaagaa
    35881 ggtttctccg cgctggcttc gaccattgaa acattggccg ccgccgagcg cctgaccgcc
    35941 cataaaaatg ccgttacttt gcgtgttaac gcccttaagg agcaagcatg agcaccgtga
    36001 ctattaccga tttagcgcgt gaaaacgtcc gcaacctgac gccgtatcag tcggcgcgtc
    36061 gtctgggcgg taatggcgac gtctggctga acgccaacga ataccccact gccgtggagt
    36121 ttcagcttac tcagcaaacg ctcaaccgct acccggaatg tcagccgaaa gcggtgattg
    36181 aaaattacgc gcaatatgca ggcgtaaaac cggaacaggt gctggtcagt cgtggcgcgg
    36241 acgaaggtat tgaactgctg attcgcgctt tttgcgaacc gggtaaagac gccatcctct
    36301 actgcccgcc aacgtacggc atgtacagcg tcagcgccga aacgattggc gtcgagtgcc
    36361 gcacagtgcc gacgcaggac aactggcaac tggacttaca gggcatttcc gacaagctgg
    36421 acggcgtaaa agtggtctat gtttgcagcc ccaacaaccc gacagggcaa ctgatcaatc
    36481 cgcaggattt tcgcactctg ctggagctaa cccgcggaaa agccattgtg gttgccgatg
    36541 aagcctatat cgagttttgc ccgcaggcat cgctggctgg ctggctgacg gaatatccgc
    36601 acctggctat tttgcgcaca ctgtcgaaag cttttgctct ggcgggcctt cgttgcggat
    36661 ttacgctggc aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc ccctacccgc
    36721 tctcgacgcc ggttgccgac attgcggccc aggcgttaag cccgcaggga atcgtcgcta
    36781 tgcgcgaacg agtgacgcaa attattgcag aacgcgaata cctgattgcc gcactgaaag
    36841 agatcccctg cgtagagcag gttttcgact ccgcaaccaa ctacattctg gcgcgcttta
    36901 aagcctccag cgcagtgttt aaatctttgt gggatcaggg cattatctta cgtgatcaga
    36961 ataaacaacc ctctttaagc ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc
    37021 agcgcgtcat tgacgcctta cgtgcggagc aagtttgatg agtcagaagt atctttttat
    37081 cgatcgcgat ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg accgttttga
    37141 caaactcgcc tttgaaccag gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta
    37201 caaactggta atgatcacca atcaggatgg tctgggaaca caaagtttcc cgcaggcgga
    37261 tttcgatggc ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg tacagtttga
    37321 tgaagtgctg atttgtccgc acctgcccgc cgatgagtgc gactgccgta agccgaaagt
    37381 aaaactggtg gaacgttatc tggctgagca agcgatggat cgcgctaaca gttatgtgat
    37441 tggcgatcgc gcgaccgaca ttcaactggc ggaaaacatg ggcattaatg gtttacgcta
    37501 cgaccgcgaa atcctgaact ggccaatgat tggcgagcag ctcactaaac gagaccgtta
    37561 cgctcacgta gtgcgcaaca ccaaagagac acagattgac gttcaggtgt ggctggatcg
    37621 cgaaggtggc agcaagatta acaccggtgt tggcttcttt gatcacatgc tggatcagat
    37681 cgccacccac ggtggtttcc gtatggaaat caacgtcaaa ggcgacctct atatcgacga
    37741 tcaccacacc gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa tggctcttgg
    37801 cgacaaacgc ggtatttgcc gctttggttt tgtgctaccg atggacgaat gccttgcccg
    37861 ctgcgcgctg gatatctctg gtcgcccgca cctggaatac aaagccgagt ttacctacca
    37921 gcgcgtgggc gatctcagca ccgagatgat cgagcacttc ttccgttcgc tctcatacac
    37981 catgggcgtg acgctacacc tgaaaaccaa aggtaaaaac gatcaccacc gtgtagagag
    38041 cctgttcaaa gcctttggtc gcaccctgcg ccaagccatc cgcgtggaag gcgacaccct
    38101 gccctcgtcg aaaggagtgc tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat
    38161 ctgaactcgg tgaagtcagc cattgcgcgc cacggttatg aaccaaaagt cagccgtgac
    38221 ccggacgtgg tgttgctggc cgataaacta tttttacccg gcgttggcac tgcgcaagca
    38281 gcgatggatc aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg tacccaaccg
    38341 gtactgggca tctgcttagg gatgcaactg ctgggtcggc gcagcgaaga gagcaacggc
    38401 gtcgacttgc tgggcatcat tgacgaagac gtgccgaaaa tgaccgactt tggtctgccg
    38461 ctgccgcata tgggctggaa tcgcgtttac ccgcaggcag gcaaccgcct gtttcagggg
    38521 attgaagacg gcgcgtactt ttactttgtt cacagttacg caatgccggt caatccgtgg
    38581 accattgccc aatgtaatta cggcgaaccg ttcaccgcgg cggtacaaaa agataacttc
    38641 tacggcgtgc agttccaccc ggagcgttct ggtgccgctg gcgctaagtt gctgaaaaac
    38701 ttcctggaga tgtgatgatt attccggcat tagatttaat cgacggcacc gtggtgcgtc
    38761 tccatcaggg cgattacggc aaacaacgcg attacggtaa cgacccgctg ccgcgcttac
    38821 aggattacgc cgcgcagggt gccgaagtgc tgcatctggt ggatctgacc ggggcaaaag
    38881 atccggcaaa acgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt aacgttccgg
    38941 tgcaggttgg cggcggtgtg cgtagcgaag aagacgtggc ggcgttactg gaagcgggcg
    39001 ttgcgcgcgt ggtggttggc tccaccgcgg tgaaatcacc agaaagggtg aaaggctggt
    39061 ttgaacgttt cggtgccgat gccttagtgc tggcgctgga tgtccgtatt gacgagcaag
    39121 gcaacaagca ggtggcggtc agcggctggc aagagaactc gggcgtttca ctggaacaac
    39181 tggtggaaac ctatctgcct gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg
    39241 acggcacgct ggcaggctct aacgtctctt tatatgaaga agtgtgcgcc agatacccgc
    39301 aggtggcatt tcagtcctcc ggcggtattg gcgacattaa cgatgttgct gccctgcgag
    39361 gcactggcgt gcgcggcgta atagttggtc gtgcattact ggaaggtaaa ttcaccgtga
    39421 aggaggccat cgcatgctgg caaaacgcat aatcccatgt ctcgacgttc gtgatggtca
    39481 ggtggtgaaa ggcgtacagt ttcgcaacca tgaaattatt ggcgatattg taccgctggc
    39541 aaaacgctac gccgaagaag gtgcagacga actggtgttc tacgatatca ccgcttccag
    39601 cgatggccgt gtggtagata aaagctgggt atctcgcgtg gcggaagtga tcgacattcc
    39661 gttctgcgtg gcgggtggga ttaagtctct ggaagatgcc gcgaaaattc tttcctttgg
    39721 cgcggataaa atttccatca actcccctgc gctggcagac ccaaccttaa ttacccgcct
    39781 ggcggatcgc tttggcgtgc aatgcattgt ggtcggcatt gatacctggt acgacggcga
    39841 aaccggtaaa tatcatgtga atcaatatac cggcgatgaa agccgcaccc gcgtcactca
    39901 gtgggaaaca ctcgactggg tacaggaagt gcaaaaacgt ggagccggag aaatcgtcct
    39961 caacatgatg aatcaggacg gcgtgcgtaa cggttacgat ctcgaacaac tgaaaaaagt
    40021 gcgtgaagtt tgccacgtcc cgctaattgc ctccggtggc gcgggcacca tggaacactt
    40081 cctcgaagcc ttccgcgatg ccgacgttga cggcgcgctg gctgcttccg tattccacaa
    40141 acaaataatc aatattggtg aattaaaagc gtacctggca acacagggcg tggagatcag
    40201 gatatgttaa cagaacaaca acgccgcgaa ctggactggg aaaaaaccga cggactaatg
    40261 ccggtaattg tgcaacacgc ggtatccggc gaagtgttaa tgctgggcta tatgaacccg
    40321 gaagccttag acaaaaccat cgaaagcggc aaagtcacct tcttctcgcg cactaaacag
    40381 cgactgtgga ccaaaggcga aacgtcgggc aatttcctca acgtagtaaa tattgcgccg
    40441 gactgcgaca acgacacgct actggtactg gcgaatccta tcggaccaac ctgccacaaa
    40501 ggcaccagca gctgcttcgg cgacaccgct caccaatggc tgttcctgta tcaactggaa
    40561 caactgctcg ccgaacgcaa atctgccgac ccggaaacct cctataccgc caaactgtat
    40621 gccagcggca ccaaacgcat tgcgcagaaa gtgggcgaag aaggcgtaga aaccgcactg
    40681 gcagcgacgg tacatgaccg ctttgagctg accaacgagg catcggactt gatgtatcac
    40741 ctgctggtgc tgttgcagga tcaggatctg gatttaacga cggtaattga gaacctgcgt
    40801 aaacggcatc agtgagttgc agggtaagcg gatgcgatgt tgttgtcgca tccggcaaaa
    40861 aaaaagggca aggtgtcacc accctgcccg ttttctttaa agctgcaaag attacttcgc
    40921 gttataatta cgcagagcgt tacgccccag cacaatcccc gcgccaacca tgccgcccag
    40981 cagcaccgcc agaatcaagg taattgcctt tttcgggcta tcgcgacgaa taggtaacgt
    41041 cggtttcatc acataacggt aagcatgaat atcaagatca tcaaccttta agttttcaat
    41101 atccaacagg ttttgacgtg tctgatagta gtttggtgag aacaccaacg gacgggtcgc
    41161 ctcatgctta atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt
    41221 cacatcttca gtctgctgaa cctgtggctt tgccacctgc gcctgattcg catactgcaa
    41281 cgcttcctga atctgacgga tacgcagatc tttctgctcc tgcgcgacta cttcctgcgt
    41341 tcttaaagag tcctgcaagt ttttccgtcc cagagcaatg ttgtctttga gatccttttc
    41401 tagctcttga ttcactttat catcaacttg ctgaatgtat tgggccaact tcatttgtgc
    41461 tccctctgca gtttgcccaa cataagaaac agtcaatggt aattgctggt tcttaacaga
    41521 aggttcgatg gtaagttttt ctggttcttc ctgattatcc agcgtttctg ctaatgcaga
    41581 gaaggcagaa ctgaagcgac caattaacgt ctcctgtaaa tccgatactt tcggtgcagc
    41641 ctgaccatag ataacattca tggcattgtt atagccagca atttgcccca cgtcgggctg
    41701 agtgataatt gctgttgacg tccatttctc cttcgctact gccaaatatc caatagccag
    41761 ggcaatagcc acaatgacgg aaatgatgat tgtcatcttg ccacgccaca actgcactag
    41821 taaatcaatc aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc
    41881 tactctcatc attaccctaa ctgacggaaa tttccaagtc cgcatagttt agcgataatc
    41941 ctagtgaatg ctataggata agtgataaaa ataatgaatt attggggagg taacaaaaga
    42001 caatcagggc gtaaatagcc ctgataacaa gatgttagtc gctgccaaag agatcgcggg
    42061 tgtatacctt atctgccaca tctttaagct cttctgccat tcggttagag ataatgacgt
    42121 cggcttgttg tttgaagatg gcgagatcac gttccagccg agagttgaag aatgagtctt
    42181 ctttcatcac tggctcgtag atgatcactt caacgccttt cgccttgata cgcttcataa
    42241 tcccctgaat agaagacgca cggaagttat ctgaaccgct cttcataatc agacgataaa
    42301 tacccaccac ttgcggtttg cgtgacaaaa tggcatcggc aataaaatcc ttacgcgtgc
    42361 ggtttgcatc gacaattgcc gagatcaggt tattcggcac agactgatag ttcgccagta
    42421 actgcttggt atctttcggc aagcaataac caccataacc aaacgacggg ttgttgtaat
    42481 ggttgccaat acgcggatcg agacaaacgc cttcgataat ctggcgagta ttcagaccta
    42541 aactttctgc gtagctatcc agttcgttaa agtatgccac gcgcatcgct aggtaggtgt
    42601 ttgcaaaaag tttaatcgct tctgcttcgg tcgaatcggt gaacagggtc gggatatttt
    42661 gcttaatcgc gccttcctgt aacaacgcag caaaacgttc tgcgcgttct gaacgctcac
    42721 cgatgacaat acgtgaagga tggagattat cgtaaagggc tttaccttca cggagaaatt
    42781 ccggagagaa aataatattt tcagtacgat atttcttatg catcgcttcg gtaaaaccaa
    42841 cgggaaccgt tgatttgatg accataaccg cataaggatt tatctcaact acgtctttaa
    42901 ttactgattc tacactggat gtattgaaat aattagtttt aggatcatag tcggttggag
    42961 tggcgatgat gacataatca gcatcccggt aagcttcatt tttatctaat gtggcattaa
    43021 agtgtatttt atctgattgc aaaaactgct gaatttcctt atcaacaata ggagatatcc
    43081 gatcattcag catagcaacg cgtgacggta aaatatctaa tgccacaacc tcatgatttt
    43141 gtgcgattag aagcccgttt gacaagccta catagccagt accggaaatg gtgattttca
    43201 tttcatgctc tcagaattaa cttaactgtg aatcatgatg tttttagcat cctgataaga
    43261 gctaaatgtt ttaacgctac gttattagct ggtcaacgct acgaaaaaca attcagattt
    43321 tctttataag aatagctgat atttattaag ttaatattaa gcagtgaaat ttagtctata
    43381 agataaatgg caaaaaaaaa gcccggtgca atatacgccg ggcctcaatt ttactgttgg
    43441 ttaaatcaga ttaatccagc cattcagtat ggaacacacc ttctttatca atgcgcttat
    43501 aagtatgtgc accgaaatag tcacgctgtg cctggatcag gttcgcaggc agaacagcgg
    43561 cacggtagct atcgtaatag gcaaccgcag cggcgaaggt cggaaccggg ataccgttct
    43621 gtactgcata agcaacgaca tcacgcagcg cctgctggta gtcatcggca atttgcttga
    43681 agtacggagc cagcagcagg ttagcgatct gcggattttc ggcatatgca tcggtgattt
    43741 tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa aatcttcgcg atttcgccgt
    43801 agttcagatc ccagttgtat tcttcagacg cagcacgcag ctgagagaag ccctgagcgt
    43861 aagaaacgat tttgcccaga tacagcgcac ggcgaacttt ttcgatgaac tcagccttgt
    43921 cgcctgctga ctgagcttgc ggaccagaga gaactttaga tgcggcaacg cgctgatctt
    43981 tcagagaaga gatataacgt gcaaacacag actcggtaat cagcgacagc ggttctccga
    44041 gatccagcgc gctctggctg gtccatttac cggtaccttt gttagccgct tcatccagga
    44101 tcacatcaac caggtagtta ccgtcttcat cttttttagt gaagatatct ttggtgatgt
    44161 cgatcaggta gctgctcagt tcaccgttat tccactcggt aaaggtctgt gccagttctt
    44221 cgttagagag attcaggccg cctttcagga gagaataggc ttcagcaatc agttgcatat
    44281 caccgtattc aataccgttg tgaaccatct tcacataatg gcctgcgcca tcagcaccga
    44341 tataggtaac gcacggttcg ccatcttcag caacggcggc gattttggtc aggatcggcg
    44401 caaccagttc ataggcttct ttctggccac caggcatgat ggacggccct ttcagcgccc
    44461 cctcttctcc gccggaaaca ccggtaccga tgaagttaaa gccttcggca gaaagctcac
    44521 ggttacgacg aatggtgtcc tggaagaagg tgttaccacc atcgatgatg atgtcgcctt
    44581 tatcgagata tggtttgagc gaatcaatag cagcatccgt gcctgcacct gctttcacca
    44641 ttaacaggat gcgacgaggc gtttccagag attcaacaaa ctctttcacc gtatagtaag
    44701 gaaccagttt cttgcctgga ttttcggcaa tcacttcttc cgtcttttca cgggaacggt
    44761 tgaaaataga gacggtataa ccacggcttt cgatgttgag cgcaaggttg cgccccatca
    44821 ctgccatacc gactacgccg atctgttgct ttgacattgt ttactcctgt cagggggtgg
    44881 tcaccgccga gtgtgcggct taaatgtggt atagatgtta actcactcat agagatggtg
    44941 aatagcatta cctttgctat accacactga atatctcacc ttttccgtct tttaaaactt
    45001 atttatatga aattactgaa ttacttgttc agtaactcaa gaataagttt tgtcttttct
    45061 tccatcagcg gcacatcacc gcgagattcc acattcaacc gcaccaccgg ttcggtgttg
    45121 gaggtgcgca ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc ggtgcgatcc
    45181 accgccagcg cctcgcggct aaaatgctgt tccacgcggt taatcgcctc aaagggttgc
    45241 gccagtttgc tgttgatctc accgcttgcc ggaaacgctg ccatccggtc gcgcaccagt
    45301 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    45361 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc accaccgtag
    45421 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacatcacc
    45481 ggcgtgccgc ctgcggcagt caccacatca acggtgttcc aggagagacg cggatcgtgg
    45541 atgatcttcg cgccgggatt tttttcgagg aatgcttctg ccagcaggcc gacaatgtag
    45601 tagccctcaa taaactgccc tttttcgtca aacaggaagc agcggtcaaa atcgccatca
    45661 aaagcaatgc ccatatccgc accgtgtttg atgaccgcat tgcgggtgtc gtcgcggcat
    45721 tccggcagta gcgggttagg aataccgttg gggaaattgc cgtccggcgt gttgtgcact
    45781 ttgattaatt ccacgggtgc gccaagggct ttaaagcggg cttcgatggc gtccaccacc
    45841 ggacccgctg cgccattccc ggagttaatc accagcttga gcggcgtgag gtttttgaca
    45901 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca gattgatttg ctgatagcga
    45961 ccgcgtttgg tttcatcaac gggaggaaag tcgttggctt ctgccagacg ctggacatcg
    46021 cgcagtccgg tatcaccgct gatcgggcga gccccttcgc gcaccagttt catgccgttg
    46081 taatccatcg ggttatggct ggcggtaact tcaatgccgc catccacgcc gagatggaac
    46141 gtggcgaaat agatctcttc ggtgccggac atgccaatat ccagcacatc gacgcccgca
    46201 tcctgtaaac ctctcgccag cgccagtttt aaggtttcgc tggtgaggcg gacatcaccg
    46261 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    46321 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    46381 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    46441 tttgtttttg tcgttattta catctcggtt tcgcattcat tgcctgatgc gacgtttaca
    46501 cccgtccgta gcgatccgcg aaacgcacca catcatcctc ttcgagataa gagccggagc
    46561 gcacttcaat taaatcgagc ggaattttcc ccgggttttc caggcaatgc atcgccccca
    46621 gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca atggtgactt
    46681 ttgccgttcc cgcgacaacc acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca
    46741 agccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag
    46801 agtcatattt gccccacggg cgatagactt cgcgatgcac ccgatgctca tggcgaccat
    46861 cggctttgat ctgctcgacc acttttttca catcctgcac cgcgttacgg tcggcaatca
    46921 gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg gttacgaggc
    46981 cagattcggc gtacacatag ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa
    47041 cgttgccctc ggcggtgtgg gcgctaattt cccataatga ggaccaggag ccgacatcgc
    47101 tccagcccgc atccatcggc accacaacgg catctgccgt acgttccatg accgcgtaat
    47161 ccaccgactc ttccgggcag gcgagaaacg cttcttcatc cacacgaata aaatcgagat
    47221 ccggatcgac ggcgctcatc gctttttcac aggcatcgag aatatccgga cgatattttt
    47281 tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc cagtaatatt
    47341 cgccgcttgc cacataggcc tgggcggttt ccagattcgg tttttcgaca aactgcgcca
    47401 cttcaaaggc caccgtatcc tgctcacccg cagacacttc accgcgacga atatagccat
    47461 aaccggtttc tggtagatcc ggcacaatgc cgaaggtcac cagcttgccc gcttcggcat
    47521 atggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatc acatgatccg
    47581 ccgccaatac cagcattaac gggtcgctct ccgggctatg acgttttgcc gccagcgccg
    47641 ccagcgcaat ggcaggtgcc gtgtttcgcc ctgccggttc gagaataatg ttctcggtaa
    47701 gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca
    47761 ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga
    47821 gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc
    47881 ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc
    47941 ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagtgtgc gttcggcata
    48001 ttcacgtgcc accgtgttgt gtttgggcag caggagcgcc tgacggatcc ccgccaccag
    48061 cgcctcgacc gattccggtt ctacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg
    48121 ccccagttct gtgtgggcct ctgcggtaat caccgcgtta ccgcctaccg ccagaatatt
    48181 ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt ttttgcacca ccagatggca
    48241 atcgcccatc ttcagcagtg cgggtaaagc gtcatacgat tgcagcggga aaaattgcat
    48301 gttgcgcagt ccacgctgct gcgccatttt ttccagccgc gctttgccgc cgccctgccc
    48361 gacaatggca aaaatcagcg gttcatcgcg cagacgatcg gcagcttcaa taacgttttc
    48421 cagcccctgc ttttcaccaa tattgccgga gtaaagaatg atttttttgt tatccggcag
    48481 gtcaagctgg ttacgaaggg cgtcaacatc ggcatctgca acatgctgaa aacgggcaat
    48541 ttccgaccag ttggggaaga agatgacgtt ttccgccgcc acgccttttt cgatggcttt
    48601 attcatcatc gaacgcgaaa tcgtggagac gttatcgacg ttatgcagtc cgctacgttc
    48661 gaacgccgtt gccagctgtg ccactttgct gcctttgcct tttccggcaa ggcccagccc
    48721 cagcatggcg tccacttcgt aatcctgaat atgcagcacg gtacgcgcac cagaaagttt
    48781 cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc accacgccga taatgcgatc
    48841 cggcttccag cgacgttgcg ccatcagagg aaagaaactg ctgacggcaa aactgccgag
    48901 atgtaacagg cgtttcaggg tgctcggctg ttttggcacg tacagcgggc agcgccacac
    48961 cgtggcggcc ccctcttctc gtttgtaccg ccaggcggaa tagttctcgc ccacctgcca
    49021 ctgcgggtag taaggcggtg cggtaataac ccgcacctca tgaccttgtg ccgccagcca
    49081 ttccaccatc tcgccggtgt atttgccgat accggttaac tccggcgagt agttaatgcc
    49141 gtagaccagt attttcataa tccgggtact ccggcacgct tctcagcgag aaaataggcg
    49201 cggctgttag cgtgaacatc gttactggcg agcagcgcgt ccggcgtcag ccagcggtaa
    49261 tcgtcatgct gctcatccgg cagtatcagc tcttcttcag cgactctgaa gcgaaaaccg
    49321 agcaccacat agtgagtggt gaaatccgtg ccggagaagt tatcgtcata aaagtgctgc
    49381 cagacaccgt aaaactggcc tgctgttatc ggcagacgca gccccagttc cgccatcgtc
    49441 agccgctcga atgcggcttc cagcgtttcg tctttctgca cgcgccctcc cggcacaaac
    49501 cagtaaccct gcgccgggcg gttggttctt ttgccaagca gaaactcacc gcgactgttc
    49561 tcgacaataa agtcgagaga gacaagcggg gtggagcgca ctaccgtggc aaagtcttcc
    49621 tgacgtaaaa acatcattac ccccgaaagc ggtcttgatt ctcaaggaac cactggtaag
    49681 tgctggcaag ccccgcttcc agtgagattt cgtgatacca gccaagctga tgcaggcgcg
    49741 tcacatccag cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa accacccgac
    49801 ctttgtaacc caccactttg gcgatggttt gcgccagctc gcggatagtg cagtcaacgc
    49861 ccgtgccgac gttaatgtgc gacagcatcg gctgggtgtt ctccagccag acttcgtgcg
    49921 ccagctccat gacatgaatg ctcgccgccg ccatatcatc gacgtgcagg aattcacgca
    49981 tcggtgtacc gctgccccat accaccacgt ccggtgcatt ctgtgccgtc gcctcgtgga
    50041 agcgacgcag caatgcaggg atcacatgcg aattactcgg gtggaagttg tcatgcgggc
    50101 catacaggtt ggtcggcatg actgagcggt aatcgcgtcc gtactggcgg ttgtatgatt
    50161 cgcacagttt gatcccggcg attttggcaa tggcgtaagg ctcgttggtc ggctccagcg
    50221 tgccctgcaa taactcgctt tctgccatcg gctgttttgc cagtttcggg tagatacagg
    50281 acgatccaag aaacagcagt ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc
    50341 tctcaatcat catgttctgg tagatgaaat ccgccggata ggtgttgttg gcaacaatgc
    50401 cgcccacttt cgccgccgcc agatagacct ggtcaatgcg ttcgctggca aagaaatcat
    50461 gcaccgcgcg gctgtccagc aggttcagct cgtcgcgggt gcgtaatacc agttccacat
    50521 caccgcgctg ttcgagctgc cgcctgatgg cagaaccgac catcccacga tggccagcga
    50581 taaaaatgcg ttgtttactc atgcttatga ctccagcgcg atcgccacgt cgtagccgtg
    50641 agatttcagc agagagtgtt ttttcgccgc ttcgaggtca ttagccacca tttcagacac
    50701 catctctctg agggtgattt ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc
    50761 gccgagcagc gtttcaactt cagccggacg gaagtaacgc ggatcaacgg cgataatcac
    50821 atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc
    50881 ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc tgtgccgctg ccatttccac
    50941 gaactgacgc acggagtact gaacgccggt cgcgataacg aaatcttccg gctgttcctg
    51001 ttgcagcatc atccactgca tttttacgta gtctttggcg tggccccagt cacgcaggga
    51061 atccatattg ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg
    51121 ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg gattcatggt tgaagagaat
    51181 tccgttacaa gcgtacatgc cgtaggattc gcggtagtta acggtgatcc agtaggcgta
    51241 cagtttggcg accgcatacg gagatcgcgg gtagaacggc gtggtttctt tctgcggaat
    51301 ttcctgcacc agaccataca gttcagaggt ggaagcctga tagaaacgag ttttcttttc
    51361 cagaccgagg aagcggatcg cctccagcag gcgcagcgta cccatcgcgt cgacgtcagc
    51421 ggtatattct ggtgactcaa aagagaccgc aacgtggctc attgcgccca ggttgtacac
    51481 ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac tcaggtcgcc
    51541 ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg
    51601 ctcggtgttg aatgacgatg cgcgacgctt aataccatgc acctcgtaac ctttttccag
    51661 cagaaactct gccaggtaag aaccgtcttg tccggttaca ccggtgatga gagcgacttt
    51721 tgacatgtct tattcctctg tatttttgaa tttattcagt ttcaacgcgt tcgcgtatca
    51781 ccactgcggg attcccccgg caaaccacat ttgccggaag cgatttaaaa acactgcttc
    51841 gtgcacccac gacggtgccg tcgccgattg tgacgcctgg ggcaacaaag acatcggttg
    51901 ccagccagca tttctcgcca atcacaatag gcgtggcgtt aatggtgaaa tgttgacttg
    51961 catggtcgtg gctaccggtg cataaataac ttttttgcga tatcaccgaa tgtgcgccaa
    52021 tggttatttc accgagggta tataaattga cgtcatcgcc gacccacgcg taatcaccta
    52081 aggttaattt ccacggatag gtaattttta ctgacggacg aataactacg ttttttccta
    52141 tttttgctcc gaataaacgt aataaaaaag cccgccagcg atacaatact tgtggcgacc
    52201 aggcaaatat tgttgcctgt actgcccacc ataattgcac tttaatagcg ttgccgcccc
    52261 ggaacccttt cggcaccgag aaaccgctta aatcttgcat tggttttcct tatattcaga
    52321 ctttgttata taaggctttc gtctttgagg tagtacgttg gcgtaaatgc caggataatt
    52381 cagcccaaaa gccaggcaca tgtaatattt gtcgttggac ttttttcgcg tcagcacaca
    52441 attccatatt attggtggta gataccccac ccatggaaaa ttcagacacc aggccattga
    52501 gttttttaaa tgcataaccg gctttataca ttttggctgc cagcgcgtag tcggaagaaa
    52561 ctttatattc caggtcataa cgccattttt tcaagccgga tacagggaaa aatatcgcct
    52621 gatgactggc gggcaggctg tgataaatat accagcccgg tttggcgcta cgtttaattt
    52681 tatgcccgtc gccaaaatcc agcagcgcat cgccggtgat catcatgtta tctttttgca
    52741 tttttaactt acggacaaaa tttgcggcat cctgatgaaa aatatcgccc gaattgagaa
    52801 acaacgcgaa cttgccttgt gccatcgtaa tgcctttgtt catggcgtcg tagataccgt
    52861 tatctggctc gctgacaaag cgtaggttat agataccatt gagattttcc agatactcac
    52921 gggtgccgtc attggaaccg ccatcgacaa caatccattc gaagctgata tcttccgcct
    52981 gcgccagatg cgccagcgag gcatgtgttt tgactatccc ttcgaggtta cgaaacgcga
    53041 cagtgattat gctaagcaac atgttcttat tcttacctcg taatatttaa cgcttttcgc
    53101 aaaataaacg gacagacgat taaaaatgca tattccgggc taaatatcga accggtaaaa
    53161 aacagcgata ccggagtaaa aagatataat tgcacgcgaa aattgcggtt atcaccaaaa
    53221 gcgttgatca tcatttttat cactttcccc atgtaccaca gcgataaaaa cactgcgaac
    53281 caggaaaaat aaataatcag cagatacaaa ccattgtcta tggtttttcc gacatccgca
    53341 ccgttaaata ttccgaatga tgcgacgtat tcataaagtg agccaaatct gactacaccg
    53401 tcaatatggg tcaaagaata accgaccatc accagcggac cgacaatacg ataatacgat
    53461 gacgatcctt ccgtacctaa atcgcccaga cgggtggaaa tataaggaaa cgcgattacc
    53521 acaccaaccc ggaatacagc cagagaaatc aatgccaacg gtaacttttt cttaatcgcc
    53581 tctttattca gatattgaaa tgcccactcc agcaaataaa acaggataaa agtcataacc
    53641 cctgaaaacg atccggataa tattatccct gcgagaatca tagcatcggt tttaggcgtt
    53701 ttgataccaa actgtttgat gctgagccaa attgagatta atgccagagc gaaaaatgcc
    53761 ggttcgaaat aaagtgctgt cgtgcgcttg ccgccgaatt taatgaaatt cagtacatag
    53821 ctgttgctgt aaatcagata tttcgaaatc gactccataa tactgctgcc accggtgagg
    53881 ataatttgcg ccatctccac cgccgccagc gccaccacca gcccgatcac cagataaaag
    53941 aaacgtaata tcttgcgatg gttgtgtggc gaaatcgttt taaagcgaat actccacacc
    54001 atgccaataa tgatcacaat atagacaaac agcatggttg aagtgacgta tttactggca
    54061 tccagcgact gaccaaacag atagttaaac gccgtgagtc ccgcaccaat ccctaaggca
    54121 atcatcaatt ttttaacgct gatgcgttct aaaaacagca gtaacaagac aggtaaaaaa
    54181 gtgacgatgg tgatggggaa actttcgcca agctgggcga ttttgacgtt aaccagcagg
    54241 tagatcagcg gcagcagcag gtagctacag attctgatag aagttgacat actcctccag
    54301 catctgttgt ccactgtagg tggcgcggct gcgttggctg aactcagcca gcgtggtacc
    54361 aaatatcgcc tgcgcgattt ccggtttgct taactgcacc agttgcagca cgtcttcttc
    54421 gtcgacggtt ttaccgccgg atttttgcaa cacttcccgt gccgcgtcgc tatgggtggc
    54481 aatcaccggc acgccaattg ataacgcctc acacaaaatc agcgggtagt tatcgacgcg
    54541 agaactgaat accagcgcat ccatctgatt gagcgcgctc atcaacttgc gcttgtcagt
    54601 ttcaaagccg tgattaacca cgttgccagc ggtgaacggc gagaacttac caaaggtatg
    54661 cagttcgatt ttgtcgccca gcgccatcat ctcacgcacc agttgctggt tagtcttgcc
    54721 gtcgtaacgc aagtcatgcg ccaccaccgc gattttcggc ttgccctggg tttcgcgcac
    54781 cggaggcaag tccgccagaa tcgcttcggt tgccatatca ataccattat tgataatccg
    54841 gcaacgccct ggaccgtaca ggctattgaa agcgtcagcc acatgctggc tgggggaaat
    54901 aaactgacag cccagcgcca gcatctcacg gaataactgg cgtttgcccg ccaccagctg
    54961 gtgtgcgcga tcaatcttca ccggcggata gttatttaag gtcgggcatt tctggcagcc
    55021 cgttttccag ccttcgcaac cgtcggtaaa ggcgcagcgt ccggtaacac tccagtggtc
    55081 gtgcagcgtc cagaccagag tgacgtccgg tttatggttt ttcacttttt cgcaaaagcg
    55141 caccacgctc ttaagattta gccagtagct gtgcagcaca tgaaaatgca ggaccaccgg
    55201 acccggtgtg cgagtaatgg tgcgatataa ctcattgaaa ttgccaaaca gatcgcgatt
    55261 aaacagacga aacagggcaa tattcgccat cgcggtcatc cgcggcgtat gtttgatgac
    55321 ctgcggatag ttctgatggc tgacgctctc tttgccgcct ttgccataac cgtagacaaa
    55381 atgtgacgcc agcccctgtt gcagcgcacg ctggtggaga tctaacgcca cacctgctgc
    55441 cccgccttcc gccagtcgca cattaaattg cagaatattc atttaattac cttcactcgc
    55501 gctttttctc ccaccaccag cgcgttgtcc gggacagagt cgagcaccac gctgcccgca
    55561 cccacggtga cgttgttacc aagcgtgata tcaccaagaa taatgacgtt ggcaccgagt
    55621 tcgacgccgt tgccaatgtg tggacatgcc atgttatcgg caccacgatt gccgatagtg
    55681 acgccgtggc gaatggtgaa atcatccccc gctaccacgt ttttattgat cactacggcg
    55741 taaccgtgat ggatagtaaa gcggcggcca atggtcgcgg cggcctggat ttcataaccg
    55801 aaaaagcatt cggtgataat gcgatacagc accagcagcg gggccgccca cagattgttg
    55861 aggacgttct ttttgcgcca caccgaacaa aaatgagcaa cacgataggc aagaaccatg
    55921 cagcacgggc gtaaactcca gctgttggca cgcagatctt ccagcatctt tagcgccccc
    55981 gaataccatc agccagacgt ttgccgttac gcaccgacag cagcgtcagc aaagtgcgcc
    56041 aggtcatacg tttattgcgg atctggtaga gggtaaacag ctgatatttt ttgcttgcac
    56101 ggtcaaattt atctttgtgc tttcggtaaa agtggaagta cccggagaat tttttcggtg
    56161 acgaggtgat ctgcatttca ccgtgattga tatgcaggat ctgcgtcgct tcttccactt
    56221 tccacggttc gccgtactcc accaccatcc gcaggaagat gtcgtaatcc tgtgctgctt
    56281 tcagttcggt atcgaacaaa cactctttga aacgccatgc ccaagtaaag acctgattgc
    56341 caatgatatt gcgtttatag aacaggcggc gtgagtacgg cgatttggga tacagtggta
    56401 agcttgccgg ttgggaataa acttctccct ggcatacgta gtcgttagcg tacaaaaagg
    56461 cgtgagtgac cagttgctgt ttatgggcga ggaagacgct cagacggttg ggtgtccatt
    56521 cgtcatcgtc atcgatcccg gtaatgtatt ccccttgcgc cagcataatc gcctggttac
    56581 gtaccgcgca cgccccgctg ttaatgtcgt tgtgaatgta ggtgatacgc ggatcgttga
    56641 gggcagtgac gtactgttgc agttgttccc aggaggtgga gcaatcatcc acgatgatca
    56701 tctcccagtt gctgtagtcc tggcgcagga ccgattttat cgcccgaatc gccagttgtt
    56761 gacggttcca ggtcggcata tagattgaaa tcagcggatt ctctttcata ggttgttctc
    56821 ccggcttatg agctggtttg cttgccggat gcggcgtgag cgccttatcc ggcctacggg
    56881 gcggtgcgaa tgcaggcgtc gttatttcgc atccgactta tattcgtatt cgtaatagcc
    56941 ataatcctga tacgcgctgg cgcggcggaa gatggagttc agaatcaccc ctttcaccgg
    57001 aataccgttt tgctcaaagc ggctcagact ggtttccact tctttcaatg tgttgaccgc
    57061 atagcgcgcc accattaacg tggttccagc gtgacgcccg acaattgctg catcggtcac
    57121 tgccagaatc ggcggcgtat caatcaacac caggtcgtag tttttactcg cccagctcac
    57181 caattcggca aagcgttcgc tcatcaacag ttcagaagga tttggcggca cctgaccgcg
    57241 cgggatcagg tcaaatttgg caatagaggt cggtttagct gcggtagtaa tatcgccctg
    57301 gccaatcaga atttccgaca ggccattgac gttattggta cccaacagct cgtgggtgta
    57361 gcctttgcgc atatcgcagt cgatcaacaa cacccgttta ttggtctgac tgatcaccgc
    57421 cgccaggttg gcgcagacaa aggttttacc gattgacggg ctcacccctg tcatcatcaa
    57481 cacattgttc tgcgcctgca tcatcgcgaa gtgcagactg gtacgcagac tgcggatggc
    57541 ttcaatcgcc agatcggttg gattccccac cgccagtagc tggctctgtt tatagcgttt
    57601 aatccctttg atggttttga cgctatcgcg cgctttctgc cattccgaca gcgggatgct
    57661 ggcatagacg ctgataccat gttcttccag cacctgcggg ctttcgatgc cgcgattaaa
    57721 caacgagcgc agcagcacac ccacgataga gagcatcagg ccgaggataa ttgcgccgag
    57781 gataatcagt cctttcttcg gtttcagcac gccaggctgg gtgattgccg ggtcaacaat
    57841 gcgcacatcg ccgacggtgc tggcttcggt gattttcagc tcctgctctt tattcagcag
    57901 ttgcatatag acctgctgac cagactcgac atcgcgggtc aggcgcacaa tctcctgctg
    57961 ggttttcggc atcgccgtta cgcgaccgtt aagtttggct ttctcttctt ccagcgcctg
    58021 acgtttctcc agcagcgtgc ggtacgccgg gtgaactttg gtgtacagct tggagatttc
    58081 cgcctcttta aaggtcagtt cgttcaactg cgcgtcgatg ttcaccatcg aatcgagcac
    58141 cgctttcgct tccagcggca gatcaacaga atctttatcc tgacggaagg cattcagttt
    58201 gttttcggca acatcgaggc ggctacgtac ttccggtaac tgttgcgcga ggaaagcgag
    58261 gcttttcgac gcttccgccg atttgcgctc aatattttgt tcctgatagt tacgggcgat
    58321 gctgttaaga atgtcgcgga tctgttcgcg atcttcaccg gtataagtca ggctcagtac
    58381 gcctgcgtct ttgccgttct ccgttaccgt caggctgttt tgcagttggt tgatcatccc
    58441 caacgtggag tatttggtga cggtaaactc actgcccggg cgggcgtgaa tggcttcaac
    58501 catcagcgtg acgccttctt ttttcagcat ctggcccgct tgcccacggg cgctaaagcc
    58561 gccatcactg ctcagggtgt agtttttatt gtccagcaca ttaagcgtaa acacctggcc
    58621 cgccatctct ttcgggcggt taaaggtagt cactttcacc gtctcgttct ggcgtcccat
    58681 cagtcgctcc cagcccgccc cgaaaatcgg gaacgtgttt ttgctcactg caatatcgag
    58741 gtcgagatcg tccaccgttt taccaagcac cagacgcgaa cgaatcaact ggatctcggc
    58801 gtccgatgca ggcggtttgt tggctaatgc cgaaccaatg tcctgcacta acgaattgcc
    58861 gctgttttgc tcgatttgta ccagtgcgtc ggcactataa atcggcgttg cgaagaaggt
    58921 gtaaaccacg gcacagaggg caaatacagc ggtgatgcca atcacccacc agcgcgcttc
    58981 aatgacggtg ccgaccaggc gaccaatatc gatttcatca ctgcccgtta ccggagcggc
    59041 atgttgtttt actttttctg tcattcttat acctgctctg cgttcaatgc ctgcgcccac
    59101 tggcgggcag accgttcaag taatgtgtac accgctgcaa acgtttcccg gcttttgcga
    59161 tacggatcgg ggatttcaca ttcgttatcc cagtgaccaa acagcatcac tttgccgcgc
    59221 atctcaggtg ccatctcgca taagcgttcg atatggcgct tttccatggt caaaatcagg
    59281 tcatagttgc gacacaggcg gcggctgatt tgtcgtgcac agtggccttc cagagacagt
    59341 tgatgttctg cggcgacgct gatagcggta ggatcagcgc ccttaccgac cagcgcgccg
    59401 agtccagcgg actccacttt cagttccggg tgataacgtt gcagtaagcg ttccgccgtc
    59461 ggggaacggc aaatattgcc gacacagaca actaatatgt tgttaaacat gacgattacc
    59521 agttatgaat gtcgctggct gtatccgtca tgtaacggac accgctaata gttggcaaca
    59581 actgattgat cagacggttc cagcgggaaa ccggggcggt ggtgacatac accacgtcat
    59641 aaggttgcag gcggaattct gtcgccatca ccagcgacgt ggcatcggac atatccagct
    59701 ggtagatatt ggcaatcttg ccattacgcc cgccctcgcc tttcagcgga cgaatgacaa
    59761 agatgccgct ggcgttggag gtggtcatgt cgataccttc agcattaccc agggcttcag
    59821 tcagggtcat gccgctaaag tccattttca gggtgctctg tttcttcact tcacccatca
    59881 caaatacttt cagatcatca ttacgcggca cgtagagaat atcgccggga taaagcagtc
    59941 ggttctgatt gaggtcgccg ttttgcatca gcgcctgcaa agaaatgcgt tcttcacgac
    60001 cattgtgtgt tagcactacg ttgcgccagt cagcggtgtc ggtcaggcca cctgcggcgt
    60061 tgatggcgtc gagaatagtc agtggcacgt tggtgatcgc ctgttgaccg gatttattca
    60121 cctgaccgga gatataggcc ttttgtgagc ggaaggcggc gatattaacg tccacctgcg
    60181 ggtcagcgat gtacgtcgct aagcgcccgg taatatcact gcggatttca gcgagcgttt
    60241 tcccgactac gtgaaccttg ccgatatacg ggtaaaacat agtgccgtca ggctgtaccc
    60301 agttgccggt gtcgctggag ctgcggtact gaccggctgg cgtggtgagt tccgggtgat
    60361 cccagacggt gacattcaga acgtcgcccg gcccgacgcg atactggtaa ttcgcgatct
    60421 cgctttccag cgtcatattg gggcgcgcta cattcgggcg tgggcgtaat tggtcaatca
    60481 ggcgcggggt cagcggataa acattcacca ttttgtcgag atcgaaatca gcgtcctgct
    60541 gtttgatgac gtctttgccc atcgtcgaca tattgctgcc cggaagtact gtgcaaccgc
    60601 ttatcaaggt tactgacacc aataatggca tcaatttcat tttggatttc atcattgttt
    60661 atttatcact ttggcagagt aattatcctg tgcactatta atagcaatat cgccatgcac
    60721 atttaccttg cagttaattg aataaaaatt taactggcat cagtcctaaa aaaattgatt
    60781 tcatccgcag gctattgaca gaataattca gactggtctt tcaggcatcc agacacgcta
    60841 ccgcccctgg ctttttagcc accaacacac tgatttagct taatttttca aaccctctct
    60901 gcatacaatc gttgatgaga aagggttatt gcggaaatta acttccgaat ataaggtgac
    60961 attatggtaa ttgaatattg gctctccaat aatgcaggag gaagtgttac agctaacgga
    61021 atagcaggca aggtaacaat tcggcaattg gctattttta agaattatat taagtgtcat
    61081 tcaatatggt ttttaggagt ttctttaggt tgacaatatt taatattgcg cctccacatg
    61141 cgatatttct taaataatgt tttattatta ccacttttaa ttcagggata atgtgaggtt
    61201 attaccctca aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta
    61261 tccatgatcg aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat
    61321 ggattgccga tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc
    61381 tcggcattga taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaggcagc
    61441 gcgaccgcgc acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg
    61501 cgtcaatctc ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct
    61561 ttagcgcccg cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga
    61621 tggagctgaa cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg
    61681 cgaagttctg gggcgtggtg acgcaaattg tggtactgga cgccattttc tcactcgact
    61741 cagtgattac cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta
    61801 tcgctatcag cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc
    61861 cgacgatcgt tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag
    61921 aaggtttcgg cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga
    61981 tgatcgaggc gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc
    62041 agacgctgcg ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag
    62101 atgcggaact ggacgccgaa accgcgtcca tgctggtgga tcatggtaac cagcagatct
    62161 ttaatccgca ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtaccgtca
    62221 gcagcattat gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagaag
    62281 aaatccgcca gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg
    62341 acgcagaaga tttgctcggt gttgttcacg ttatcgacct gctacaacag tctctgcgcg
    62401 gcgaaccgct caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc
    62461 cgttgttacc tgccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg
    62521 atgagtttgg ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgtcg
    62581 gtaacttacc gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg
    62641 acggttcctg gacggcgaat ggtcatatgc cgctggaaga tctggtgcaa tatgtgccgc
    62701 tgccgctgga tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gaatatttac
    62761 agcgtattcc aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc
    62821 aggtggaaag ccatcgcgtg cagaaggtgc agattattcc gctgcataag gatggcgagt
    62881 tggagtacga ggtgtgatgg tatcggtcat tttacccaat agccgaaagg aataagatga
    62941 gggaaccgcg ccagcggcga tttaccgccg ggagcccggg ttgccagggt ggtggcgatt
    63001 gagccaccct ggcacgttca ccggcttgag cgttccatag tagcgaggaa catcaagtga
    63061 acggaacagc cttcgatctt acatattcag aatataatga tgccgccacg aacgcctgat
    63121 gcgacgcttt acgcgtctta tcaggcctac aggactggtc acctggtaac caacatcttc
    63181 tccagcaact tcttcacatc cttgccattg cgggaatctt tattccgctc ggcccagtca
    63241 ttcaggcgac gtttcgcttc gtcctgtaga tgtttgcgca gtaactgatc cacttgcagg
    63301 ctgtaattga gttgctgcca gttgccataa acccgcagcg gtactggcgt ttctttcagg
    63361 aaatcaatca gtttactttc cccgttccag ccacccacga cccgaatatc aaactgggta
    63421 tcgcaggttt gatctgccag attcaacatg ccttcccctg tcagcgccag cacaggcgat
    63481 tgaccttgca tgtcgtttaa cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag
    63541 cggtcaagcc gcgtcacgtt atcaaagttt tcagcggcct tcacatcacc gccattacgc
    63601 tctaccgctt gctgaatcat ctgctggaag ttcatccctt ccatgcgcgt gtcggtcatt
    63661 tcgacatgtg cctgtccttg ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg
    63721 gagaaatcac cagccagtga catttttccg gtcaacgaaa tcggatagtt aaacgccttc
    63781 agaatggtac caatctcaac gttttccagc cgtggctgga agtttatccg cggatttatt
    63841 gatgtcgcgt ccagcgtgcc cggcagtgaa acctgtccac cgttaagttt gccctgcagt
    63901 tgagtaattt ccagcaaacc cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc
    63961 attccgcgcc agcgcacgtt actggcctgc aacaaaatat cagccgtaaa gccttgcagt
    64021 ccctgatagg ccggttcatc aatacgcgaa gaaatgaccg ggcgcggcaa cgttgattgg
    64081 ctctgcccct gctgcgcggc accgttttca ccattcgcgg tttcattaag cgggatgagg
    64141 ttgtcaagat tcagttgcgg gaattgcagt ctcagctgcc attccggttt ctctgtcagc
    64201 gtgacctgtg cttgcccgct cagcgtactg tcattggcgg tcaagctgat ttggttaaat
    64261 gaaaggcgtt tatgtgactc ctgccactgg gcctggaaac tcccctgccc ctgaatacct
    64321 tgttttggta aatcggcacc ctgcaactgc cagttaattt gttcaatagc cgccgttaaa
    64381 tcatgcggat aatcagaagc atccaccgta ccgttaaggg atattgtgag atcgcgctga
    64441 tcgcgattaa cccgcccgga gaactcaaat gagccacgat gttggggatc ttgttccatt
    64501 tgcaggcgga tattgcggat tgtcacctgc tcgtcatctt catgctggaa caccagcacg
    64561 ctgtccgcca ccttaagact ggatatatca aacgaccatc cgcgatcgtc tgacagatcc
    64621 ggcaaggtat tgtcgcgcgg tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc
    64681 ggcgtcagtt ggatcactgc cccttttagc atcacctgct taacgctcag ttgatgactc
    64741 agtagtggta aaagcgccac gtccagacgc atgttgtcgg cgcgaaccag tggctggctt
    64801 gcgccctggg cggtgagaga cattcgcccg gagaggatac taagctgcgg ccagacgtgc
    64861 caacgcagtg gcccgtcgag ctgcaattga taaccgctac gtgcagcaac ttgcttgacc
    64921 atatagtcgc ggaaatcatt cggattcacc agcaacacta acacagataa cccggccacc
    64981 agcacgacca ggagtatcat cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc
    65041 ctgaattagt ctttatcgat tcggctggct accgcgccct gctggttgcg atatttcgca
    65101 tcttcacggc ggttgtaagg tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca
    65161 ccaattaaca tgcccggacg cagcgccagc ggcagcttac cggagttgta gaactccagc
    65221 acaatgcaac cagaccagcc cggatcgata cggtgcgcgg tgacgtgcac catcagcccc
    65281 agacgcgcca aagaggaacg cccgtccagc cagcccacca gatcggctgg cagcgtcacc
    65341 gactccagcg tcaccgccag cgccagctct cctgggtgaa gatagaacgc ctcgccctcg
    65401 tcgagaacga tctcatcgct catcacgcgg tcaagcgcgg cgctcacttc atctttggga
    65461 ccgctcagat cgataaacgc tgccgtgtga ccacggaagg tacgaaattt attgcccagg
    65521 cgtacatcca ccgtcgcgcc gttaatacgc tccactggcg gacgtgggtt gatcgacaaa
    65581 cggccttcat caagccaggc ttcaatatct cggtcacaca gacgcatggc attttctcct
    65641 ttcgcgcatc actcccttaa cgccaattac gtcaagggca taccaggtta tcactgaacg
    65701 gtacacaatt tatcaagctt attcaaagaa ctgacttatt ttcgctttca atatatcgat
    65761 cgcgatgcgg tttttcccgc cgcgcggcac gataatgtcc gcatattgtt tagaaggctc
    65821 aatgaattgc aggaacatcg ggcgcacggt tttttgatat tgcgccatca cggaatccat
    65881 tgaacgccca cgctcgttaa cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg
    65941 ggtatcaacg aaaatggaga agttaagttc gtcacgcaaa cgcgcatccg tcagcaacaa
    66001 aatgccttcg agaatgatga ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt
    66061 atgttcaaca tagctgtaaa ccggcaggtc aattgccgag ccgcgtttca acgcttgtaa
    66121 atgctcaagc agcaggctgt gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg
    66181 ttcttccatc gacagatggc tttgatcttt gtaatagcag tcttcgggaa ttacgccgat
    66241 gtgttcatca ccgacttgct cacgtaattc acgataaagg gtactggcaa taagactctt
    66301 gccggaagcc gatgcgccag cgataccgat aatgacgcac tgatgagact gatcagtcat
    66361 atatttagcg acctgattaa cctggatgtt aggaaggggg cgacgaagcg ccaaacgcgg
    66421 caattatagg gatttcatcc gtctgatacc agtcgaatag cgttgccgcg cgctcagagt
    66481 taattgttga caaagaattc ccggtggcaa attacgttga tcagttttat gtaaggtaaa
    66541 aaatgttata cgcagttgcg caaattatcc gcctttacgt cactttatga gcaattcgca
    66601 tataaaatgt aaaacttttg tactagcata aacacagaaa cgaatactgg cgacctggtc
    66661 ttgcggataa agcggtaatg agcaaacaat cacagcatgt attaattgcc ctgccccacc
    66721 cgctgcttca cctggtcagt ttaggtttag tctcgtttat ctttaccctt ttctcgcttg
    66781 agctttcgca gtttggcacc caactcgccc cactgtggtt cccgacgtcc atcatgatgg
    66841 tggcgtttta tcgccatgcc gggcgcatgt ggccgggaat tgcgctgagc tgttcgctgg
    66901 gaaatatcgc cgcatccatc ctgctttttt ccaccagctc gctgaacatg acctggacga
    66961 ccatcaatat tgttgaagcc gtggtcgggg cagtgctgct acgtaaattg ctgccgtggt
    67021 ataacccctt gcaaaatctg gctgactggc tgcgtctggc actcggcagc gccattgttc
    67081 cgcctctgtt ggggggtgtt ctggttgtcc tgctgacgcc cggagacgat cctctcaggg
    67141 catttttgat atgggtactg tcagaatcca tcggcgctct ggcactggtg ccgctgggat
    67201 tgttatttaa accacactat ctgctgcgcc atcgcaaccc acggttgctt tttgagtcgc
    67261 tgctcacgtt agccatcaca ctgacgttaa gctggctttc gatgctgtat ctgccgtggc
    67321 cttttacttt cattattgtg ctgttgatgt ggagcgccgt gcgcctgcca cgaatggaag
    67381 cctttttgat cttccttacc acggtgatga tggtgtcgct gatgatggcc gcggatccct
    67441 ccctgcttgc tacgccgcgt acgtacctga tgagccatat gccgtggcta ccgtttttgc
    67501 tgatcctgct gcccgccaac atcatgacca tggtgatgta tgcctttcgt gcggaacgca
    67561 aacacatttc cgaaagcgaa acccgctttc ggaacgcgat ggaatattcc gctatcggta
    67621 tggcgttagt gggcaccgag ggacaatggc tgcaaaccaa caaagcgctc tgccagtttc
    67681 tcgggtacag tcaggaagag ctgcgcggac tcacctttca gcaactgacc tggccggagg
    67741 atctcaataa agatctccaa caggttgaaa agctgataag cggtgaaata aacacctatt
    67801 caatggaaaa acgctactac aaccgcaatg gcgatgttgt ctgggcgttg cttgccgtct
    67861 cactggtgcg ccacacggat ggcacgccgc tctattttat cgctcagatt gaagacatta
    67921 acgagctaaa acgcaccgaa caggtcaatc agcaactgat ggagcgcatc acgctggcta
    67981 acgaagcggg cgggattggc atctgggaga gggagttgaa gccgaatgtt tttagctggg
    68041 ataagcggat gttcgagctg tatgaaattc ctccacatat caaaccgaac tggcaggtgt
    68101 ggtacgagtg cgtgctgccg gaagatcgcc agcatgccga aaaagtgatt cgtgattcgt
    68161 tgcaatcacg ctcgcccttt aaactggaat ttcgcattac cgtaaaagac ggtattcgcc
    68221 atatccgcgc cctcgccaac cgggtactga ataaagaagg cgaagtcgaa cgtctcctcg
    68281 gcatcaatat ggatatgacc gaagtgaaac agcttaacga ggcattgttt caggaaaaag
    68341 agcgcctgca cattacgctt gattccatcg gtgaagccgt ggtctgtatt gatatggcga
    68401 tgaaaattac ctttatgaat ccagtggcgg agaagatgag cggctggacg caggaagaag
    68461 cgttaggtgt tccgctcctg acggtgttgc atattacttt tggcgacaac ggaccattaa
    68521 tggagaacat ttacagtgcc gacacctcac gttccgcaat cgaacaagat gtggtgttgc
    68581 actgtcggag cggcggcagt tacgacgtgc attacagtat tacgccgtta agtactctgg
    68641 acggcagcaa tattggttcg gttctggtga ttcaggacgt caccgaatca cgcaaaatgc
    68701 tgcgccagct gagctacagc gcctcccatg atgcactgac gcatctcgcc aaccgcgcca
    68761 gttttgagaa acaactgcgt atcctgctgc aaacggtaaa cagtacgcat cagcgacatg
    68821 ccctggtgtt tatcgatctt gatcgcttta aagcggtgaa tgacagcgcc gggcatgcgg
    68881 cgggcgacgc tttgctgcgc gaactggcgt cgttgatgct gagtatgctg cgctccagtg
    68941 acgtgctggc gcggctcggc ggtgatgaat ttggtctgct gctgccagac tgcaatgttg
    69001 aaagcgctcg ttttatcgct acacgtatta tcagtgctgt gaatgactat cactttatat
    69061 gggaaggccg ggtacatcgg gtaggtgcca gcgccgggat taccttgatc gatgacaaca
    69121 atcatcaggc ggcagaagtg atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg
    69181 gtggacgggg ccgggtgacg gtttacgaac cacagcaagc tgccgcacat agcgaacggg
    69241 cggcgatgtc gcttgatgaa cagtggcgga tgattaaaga gaatcagttg atgatgatcg
    69301 cccacggtgt cgcttcgccg cggatcccgg aagcgcgtaa tttgtggctg atttcgctta
    69361 agctctggag ttgcgaaggc gaaattattg atgaacaaac atttcgtcgt agcttcagcg
    69421 atccggcact tagccatgct cttgaccgac gggtattcca cgaatttttc cagcaggccg
    69481 ccaaagcggt tgccagtaaa ggcataagca tcgccctccc cctttccgtt gccggtttga
    69541 gtagcgccac gctggtgaat gatctgcttg agcagctgga aaatagccct ctaccaccac
    69601 ggttattaca tctgattatt ccggctgaag cgattttaga tcacgcagaa agcgtgcaaa
    69661 aactgcagct ggcgggatgt cggatagtgc tcagccaggt gggccgcgat ctgcaaatct
    69721 tcaactcgct gaaagcgaat atggcagatt acctgctact tgatggtgag ttatgcgcca
    69781 acgtgcaggg taatttgatg gatgagatgc tgattacgat tattcagggg cacgctcagc
    69841 gactcgggat gaaaaccatc gccgggccag tcgttttacc cttagtgatg gatacgcttt
    69901 ctggcatcgg cgtcgatctg atttatggtg atgtgattgc cgatgcccaa ccgctggatt
    69961 tgctggtgaa tagcagttat ttcgcgatta actgagagag cgtttgccca ttgcctgatg
    70021 cgacgctaac gcgtcttatc atgcctacaa atcgctcatt ccccagaccg gataaggcgc
    70081 tcgcaccgca tccggcgacc aacgtcatgc ttcgtctggt tgccagcctt ccgtatacca
    70141 gatatgcaac agcgcataag aacgccaggg cttccagcgc tcggcataac ggcggatttg
    70201 tgccggtgtc attcccggaa atcgccgttt aatcagataa tcatccggca gaaaaacatc
    70261 tttcgcctgc cagccacgca aagcaaaata attcgccgtc cagcgcccga tacccggaaa
    70321 agtttgcagc gttttcatcg cctgctccac atcgcccgga attgtcattg gtaagctgcc
    70381 ctccagcgcc gcatttgcca gatgaatcag tgcctctgcc cgtttcaacg gcatgcctaa
    70441 cgcttttaat gcctgcgggt cggctgctgc cagccgctga ggcgtgggga agcagatata
    70501 ctccggaaaa tcatccagcc gttcgccata aagctgtacc actttggcag tcaattttgc
    70561 cgccatcgcc acgctcacca gttggcctaa aatcgcccgc acaccctgct caaaagcatc
    70621 aatacagccg ggtaaacgca atccgggccg ccccgcgcct aactttccca acgcaccgtt
    70681 aacaatctgc gggttacatt gcagatcaag caggcggctc attttcgcca gacactctgc
    70741 ggcaacaggt tctaaacctg cacttaaatt tatgtgcaga gtatggcggg ctatatccgg
    70801 aatagcagtc actacgccgc gatattcgcc caccgccaga ctacgggcat aataattgtc
    70861 cgcgaccgtt tccacaccgc tcaccgcacg ggcggcgaga aatcccaaca tccacgacca
    70921 gtcatacggc ggctgccagt tcagggtata catcgcatct ccttgttaat ccgctttcag
    70981 cataaacgtt attcagacgt tacgctttgc tttcatattc cggttgtcgc gacagcaaca
    71041 tttcgctaaa gtcacgcccc ttcttcaccg gcatggggat taattcgtgt ttattggttt
    71101 tgattacggt acagcaaact gttcagtggc ggtcatgcgt gacggtaaac cgcaattact
    71161 gaaaatggaa aacgacagca cgctgctgcc ttcaatgctt tgcgcgccaa cgcgtgaatc
    71221 ggtaagcgaa tggctgtacc gccatcatga tgttccggca gacgacgatg aaacgcaggc
    71281 gctgttacgt cgggcgattc gttataaccg cgaagaagat atcgatgtta cggcgaaaag
    71341 cgtgcagttc ggtctttcct cactggcaca gtacattgat gatccagaag aagtgtggtt
    71401 tgtgaaatca ccaaaatcgt tcctcggtgc cagcggctta aaaccgcagc aggtagcgct
    71461 gtttgaggat ctggtctgcg caatgatgtt gcacattcgc cagcaggcgc aggcgcagct
    71521 gccagaagcg attactcagg cggtgattgg tcgcccgatc aacttccagg ggctgggcgg
    71581 cgatgaagca aacgcccagg cgcaagagat tctggaacgc gcggcgaagc gtgccggatt
    71641 cagggacgtg gtattccagt acgagccgat cgcggctggg ctggattacg aagccacctt
    71701 gcaggaagaa aaacgggtgc tggtggtgga tatcggcggt ggtacgactg actgttcatt
    71761 gctgctgatg gggccgcagt ggcgttcgcg tctcgatcgt gaagccagtc tgctgggtca
    71821 cagtggttgt cgtattggcg gtaacgatct ggatatcgcc ctggcgttta aaaacctgat
    71881 gccactgctg ggtatgggtg gcgaaaccga aaaaggcatc gccctgccga tcctgccgtg
    71941 gtggaatgcg gttgccatca acgacgtacc tgcgcagagt gatttctaca gtagtgccaa
    72001 cggtcgtctg cttaacgatc tggtacgcga tgcccgcgaa ccggaaaaag tggccctgtt
    72061 acagaaagtc tggcgtcagc gtttaagcta tcgcctggtg cgtagcgcag aagagtgcaa
    72121 aattgctctt tcaagcgtag cggaaacccg cgcctcactg ccgtttatca gcaatgaact
    72181 ggctacgctg attagccagc gagggctgga aagcgccctc agccagccac tggcgcggat
    72241 tctggaacag gtgcaactgg ccctggataa cgcccaggaa aaaccggacg ttatctacct
    72301 gaccggcggt agcgcccgtt ctccgcagat taaaaaagcg ctggcagaac agttgccggg
    72361 cattccgatt gcaggcggcg atgactttgg ctccgtcacc gccgggctgg cacgctgggc
    72421 ggaagtggtg tttcgttaat tgaaaaatgg ctcagcagcg gcttaaatct gcctgcgcct
    72481 gagccagttt ttctgctgcc tgacgtaacg gttccatcaa cggcatggtc cgtgctggag
    72541 catgaaggcg gaatcgctgc aactctgtcg cgccgactgt caacgccgat tccagccgat
    72601 gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt atcgcttcat
    72661 tcggcctgaa cacaaaacgg cgttcacagt tcgctttcca gtcgataacc gcctgggtca
    72721 gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct gcggctgttt
    72781 caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcaa
    72841 catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcatgaa
    72901 gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc ttcatttttt
    72961 cgagcatcgt ccgtttggcg ataaaacctt ccaggccgcc tacctgctgg atctggcgga
    73021 ctaaatggtc atagtccatt ttcgcacgat taaatgcctc acggcgttgc tgaacttctg
    73081 ctttgtacgc tttgcttgtc aaactgccaa taatccagat agccgccagc acaggaacca
    73141 aaataatata acgcggttct gcctgaaggc cgcaaagcag cagcgataat gctgacagtg
    73201 cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag
    73261 tcggttggaa atgatcgggt aatggcagtt gcaatgctgg cggtgctact gacgccatca
    73321 ccatcgccca gacttttgcc agcacaaaat ccccgccggt ggtaatgacc tcttcgccga
    73381 gatcaataaa atagataaca ccttgattat ccagcgcaca ccatgggcag tcagccaaat
    73441 gaccgggata aacatgcatt gccgaaacgg tacatttctt taactgttgg cgtagagaat
    73501 ccagtgctga tacccacgct ttagccgtcg gacgcccggt tgccacgcca ctttccgtaa
    73561 acgcctgctg aaacatggct tcaacatcac tcggtaacat cgatagcgga atcgatcgtg
    73621 gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg aaatgggtaa
    73681 tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc
    73741 ggccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattttctg
    73801 tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta aaatgcgaca
    73861 cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcgctatcaa
    73921 tcaacaccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg tcacccacga
    73981 cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta
    74041 gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggcta tagatcatat
    74101 gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt ccgcctcgtc
    74161 caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg tcagctgtcg
    74221 cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg
    74281 cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct ttgcccagtt
    74341 cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca
    74401 cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg ggctggacaa
    74461 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc
    74521 attgaaaaac ggggtaaaga aaggcacatg aggagaatta tccagcatat ttaacgccag
    74581 ccgctgaata ccatccgtaa atgctgcaac tttatgcgca cgcccagtgc tggtgaaagt
    74641 ttccagtctg gaaacggcat cttcgtcggt aatgaagtgc gtcatgttgg catattcacc
    74701 caccatcggc gtgagaggca attgcagacc gtgacccaaa tctaccacca cgccgccatc
    74761 gccaatctgc ataatcaagg tgccgtcagg cgatgaaatc aatcccagaa acgtgcaggc
    74821 aaaatcgcga actgctaatg ctttggcttc tgcctcggca aaaagtcgtt gacgaatagt
    74881 cagtaccata tttgtggcga ggacatcatt aagccccaat tcaccgtcct gtactttttg
    74941 caccataaac gccatcgctt cattgacggc gagcgtcgcg ccttctcctc cctgcgaaac
    75001 gctacctgcg ccatcagcaa ggaacatcac cagtaatggt tgctggtcgt ttaaccagac
    75061 aacctgcatc tggcaggcat cctgacaagg caaatcagca ctaatgtgcg atgttccaac
    75121 agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc
    75181 acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta
    75241 aacagttcac ggaattgtaa tccctgcagt ggcaaaggct gacgaacgct gatttgtgcc
    75301 agagtcttca tatccgcccc ctgaacaccg atggaaaaga aggcaaactt cttatcttct
    75361 tccccctgaa acactttgtt ggcggcagcc tgccactcat cggttggagc gccatcggtg
    75421 atcaggaaaa tccacgggcg ataatacgaa ataccatttg cgcggtactc acgtttcctc
    75481 tcttcaacca tatccagggc tttggtaatg gcggccccca taggcgtatc cccctgggca
    75541 aacaggatgg gcgggaaaaa gttagcggcg ctggtaaagg gttgttccac atgcaccggg
    75601 ccgaacgtca caatgccgag ttccactctt tttaaagcca gcggatcagc aagcagttca
    75661 tcacgaaagg taaccaatcc ggcgttaagt tcattaatag gtctgccgct catcgagcca
    75721 gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg
    75781 gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta tcgaggtccg
    75841 gtatcatacc gattgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc
    75901 cgtcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact
    75961 aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc cctccccctg
    76021 gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt
    76081 catcataacc ctttccttat taaaagccct cttctccggg agagggtttc ccgtttcagc
    76141 gtcccgctga aatcgtcggc ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg
    76201 tgaacaaaat agtttcatct gtgattattg cgtaacgact cgcaaagaat atttgctggc
    76261 aggatcgcag actacaaagc ctgcggattg acaatcttat cgtgaaggca taatttcagg
    76321 agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagcggg gaagcttatg
    76381 actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt aaggcccctt
    76441 cctcgggagg ggctttcccg tttcagcgtc ccgctgaaat cgtcggctta cctcctttcg
    76501 ccctgtatgc agtctatcgc tgacttgcgg ataaaatagt ttccagtgtg attattgcgt
    76561 aacgtctcgc aaagaatatt tgctggcagg atcgcagact gcaaagcctg cagattgaca
    76621 atcttatcgt gaaggcatac tttcaggagt gagggtagag cggggtttcc cccgccctgg
    76681 tagccttagt aagctggcca gctaatgact aagagcacca cgatgatgag tagcttcatc
    76741 atgacccttt ccttatttat ggccccttcc tcgggagggg ctttcccgtt tcagcgtccc
    76801 gctgaaatcg gcggcttacc tcctttcgcc atgcaagcag tctatcgcta acgcgtagat
    76861 aaaatagttt cctgtgttat tactggatgc gtgctcgcaa atgtgcccgt cattcagacg
    76921 attcccgaca gtgtttcata attcctccat ttttctccct tattggctgg ctacactagt
    76981 atcattccgc gaaacgtttc aggaagagaa actcttaacg atgaaaggca gttataaatc
    77041 ccgttgggta atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg
    77101 caatgactcc cagagtgcag ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg
    77161 tggtcgccgt ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga
    77221 acaggcagtt ccgcgttacc tcaccgggct tggcaccatt atcgccgcta atactgttac
    77281 ggtgcgcagc cgcgtggacg gtcaactgat ggcgttacat ttccaggaag gccagcaggt
    77341 caaagcaggc gatttactgg cagaaattga ccccagccag ttcaaagttg cattagcaca
    77401 aacccagggc caactggcaa aagataaagc cacgcttgct aacgcccgcc gtgatctggc
    77461 gcgttatcag caactggcaa aaaccaatct cgtctcccgt caggagctgg atgcccaaca
    77521 ggcgctggtc agtgaaaccg aaggcaccat taaggctgat gaagcaagcg tcgccagcgc
    77581 gcagttgcaa ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa
    77641 gcaggttgat gttggtaacc aaatctccag tggtgatacc accggaattg tggtgatcac
    77701 ccagacgcat cctatcgatt tagtctttac cctgccggaa agcgatatcg ctactgtagt
    77761 gcaggcgcaa aaagccggaa aaacgctggt ggtagaagcc tgggatcgca ccaattcgaa
    77821 gaaattaagt gaaggcacgc tgttaagcct cgataaccaa atcgatgcca caaccggtac
    77881 gattaaagtg aaagcacgct ttaataatca ggatgatgcg ctgtttccca atcagtttgt
    77941 taacgcgcgc atgttagtcg acaccgaaca aaacgccgta gtgatcccaa cagccgccct
    78001 gcaaatgggc aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa
    78061 acatctggtg acgccgggca ttcaggacag tcagaaagtg gtgatccgtg caggtatttc
    78121 tgcgggcgat cgcgtggtga cagacggcat tgatcgcctg accgaagggg cgaaagtgga
    78181 agtggtggaa gcccagagcg ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc
    78241 gaaaaaagga gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg
    78301 cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat cttactcgcc
    78361 gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc
    78421 attcaggtgg tcacgctcta cccaggtgcc agcccggatg tcatgacctc tgccgttacc
    78481 gcgccgctcg aacgccagtt cgggcagatg tctggcctga aacagatgtc gtcgcaaagt
    78541 tccggcggtg cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc
    78601 gagcaggaag tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct
    78661 aacccgccgg tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgccgtc
    78721 acctcaaccg ccatgccgat gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag
    78781 aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt ccggcggtca gcgtccggct
    78841 gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg
    78901 cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag gtagcctcga cggcccttcc
    78961 cgtgcggtca cgctttccgc gaacgaccag atgcaatccg ccgaagagta tcgccagcta
    79021 atcatcgcct accagaacgg cgcgccaatt cgtctgggcg atgtcgcaac tgtagagcaa
    79081 ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat
    79141 gttcagcgcc agcccggtgc taacattatc tccaccgccg acagcattcg gcagatgctg
    79201 ccacagctca ctgagagtct gccgaaatcg gtgaaggtga cagtgctttc cgatcgcacc
    79261 accaatatcc gcgcatccgt cgatgatact cagtttgaat tgatgatggc tatcgcgctg
    79321 gtagtcatga ttatctacct gtttttgcgc aatattccgg cgaccatcat tcccggtgtt
    79381 gctgtaccgc tgtcgttaat cggcactttc gcggttatgg tgtttctcga tttttcaatc
    79441 aataacctga cactgatggc gttaactatc gccaccggat tcgtggtcga tgacgccatc
    79501 gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg
    79561 ctcaagggcg caggtgaaat cggctttacc attatctcgc tgaccttctc actgattgcg
    79621 gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgct
    79681 attaccctgg cggtagcgat tttgatctca gcggtggtgt cgctgaccct gacaccgatg
    79741 atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac agaaccgctt ctcccgtgcc
    79801 tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc gtggactggc gaaagtgctg
    79861 aatcatccgt ggctgacctt aagcgtggca ctcagcacgc tgctgcttag cgtgctgctg
    79921 tgggtgttca ttccgaaagg tttcttcccg gtacaggaca atggcattat tcagggcact
    79981 ttgcaggcac cgcaatccag ctcctttacc aatatggccc agcgacaacg ccaggtcgcg
    80041 gacgtgattt tgcaggatcc ggcagtgcaa agcctgacct catttgttgg cgttgatggc
    80101 actaacccgt cgctgaatag tgcacgttta caaatcaacc tcaaaccgtt ggatgaacgt
    80161 gatgatcggg tgcaaaaagt catcgcccgt ctgcaaacgg cggtagataa agtgccgggc
    80221 gtcgatctct tcctgcaacc aacgcaggat ctgactattg atactcaggt cagccgcacc
    80281 cagtaccagt ttaccttgca ggccacctca ctggatgcgc tcagtacctg ggtgccacag
    80341 ttgatggaaa aactccagca actgccacag ctttctgatg tctccagcga ctggcaggac
    80401 caagggctgg tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc
    80461 atggcggatg tcgataacgc cctgtacaac gcgtttggtc agcgactgat ttccactatt
    80521 tatactcagg ctaaccagta tcgcgtagtg ctggagcaca acaccgaaat taccccgggc
    80581 ctcgcggcgc tggataccat tcgcctgacc agtagcgacg gcggcgtggt gccgttaagc
    80641 tcaattgcca aagttgaaca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc
    80701 ccggtaacga ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcag
    80761 gagattatgg acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcaattccag
    80821 ggcagcaccc tcgccttcca gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg
    80881 gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc
    80941 attctctcga cgctacccac cgcaggggtc ggcgcgctgc tggcattaat gattgccggt
    81001 agcgaactgg atgtgattgc gattatcggc attattttgc tgatcggtat tgtgaagaag
    81061 aacgccatca tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg
    81121 cgcgatgcaa tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg
    81181 gcggctctgc ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt
    81241 cgtccgttag gtatcggcat ggtcggcggt ctgattgtca gccaggtgct gacgctgttc
    81301 accacgccgg tgatttatct gctgttcgac cgcctggcat tgtggaccaa aagtcgcttt
    81361 gctcgccatg aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg
    81421 gcgacgattt tactgtcggt cgccattacc ctgtgcggca tactgggctt ccgtatgctg
    81481 ccggtcgccc cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc
    81541 ggtgcgtcac cagaaacaat ggcgtcttcc gttgccacgc cgctggagcg ctcgcttggg
    81601 cgcattgccg gggtcagcga aatgacctcc agcagttcgc tcggcagcac acgtattatt
    81661 ttacagtttg attttgaccg ggacatcaac ggcgcagcgc gtgatgtgca ggcggcgatc
    81721 aacgctgcac aaagtttgct gcccagtggg atgcccagcc gtccgaccta tcgcaaagcg
    81781 aacccgtcgg atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt
    81841 gaactgtacg atttcgcctc gacgcagctg gctccgacga tttcgcaaat cgacggtgtt
    81901 ggtgatgtcg atgtcggagg cagctcactg cccgccgtac gcgtcgggct gaatccgcag
    81961 gcgctgttta atcagggcgt gtcgctggac gacgtacgca ccgccatcag caatgccaac
    82021 gtgcgtaaac cgcagggcgc gctggaagat ggcactcacc gctggcagat ccagaccaat
    82081 gatgagctaa aaaccgccgc tgaatatcag ccgttgatta ttcactacaa caacggcggc
    82141 gcggttcgtc tgggcgatgt ggcgacggtg accgactcag tgcaggatgt gcgcaacgcc
    82201 gggatgacca acgccaaacc ggctatttta ctgatgatcc gcaaactgcc ggaagccaat
    82261 attatccaga cggttgacag catccgggca aaattaccgg agttgcagga aaccattccg
    82321 gcggcgattg atctgcaaat tgcccaggat cgctccccca ccattcgcgc ctcgctggaa
    82381 gaagtcgagc aaacgctgat tatctcggtg gcgctggtga ttctggtggt gtttttattc
    82441 ctgcgctcgg gtcgcgccac tattattccc gccgtttcgg tgccggtttc gctgattggt
    82501 acgtttgcgg cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcgctc
    82561 accatcgcta ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcacgt
    82621 catctggaag cgggaatgaa accgttgcaa gccgcactgc aaggtactcg cgaagtcggt
    82681 tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt tcctgccgct gctgttgatg
    82741 ggcggattgc cgggccgact gttacgcgag tttgccgtga cgctttctgt cgccattggt
    82801 atttcgttgc tggtttctct gacattaacg ccaatgatgt gtggctggat gctgaaagcc
    82861 agcaagccgc gcgagcaaaa gcgactgcgt ggttttggtc gcatgttggt agccctgcaa
    82921 caaggctacg gcaagtcact gaaatgggtg ctcaatcata cccgtctggt gggcgtggtg
    82981 ctgcttggca ccattgcgct gaatatctgg ctgtatatct cgatcccgaa aaccttcttc
    83041 ccggagcagg acactggcgt gttgatgggc gggattcagg cggatcagag tatttcgttt
    83101 caggcgatgc gcggtaagtt gcaggatttc atgaaaatta tccgtgacga tccggcagtg
    83161 gataatgtca ccggctttac aggcggttcg cgagtgaaca gcgggatgat gtttatcacc
    83221 ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgta
    83281 aaactggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt
    83341 ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca
    83401 ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac
    83461 gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc
    83521 atggcacggc tgggaatcga cgtgcaggcc gccaacagcc tgttaaataa cgccttcggt
    83581 cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg
    83641 gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa
    83701 ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaacgc tccgctgtcg
    83761 gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc gaccggaaaa
    83821 tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg
    83881 acggtgcgcg gcagttttgc cggaacagcg caggtgttcc aggagacgat gaactcacag
    83941 gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag
    84001 agttacgtac acccactgac gattctctcc accctgccct cggcgggcgt tggtgcgctg
    84061 ctggcgctgg agctgttcaa tgccccgttc agcctaatag ccctgatagg gatcatgcta
    84121 ttaatcggca tcgtaaagaa aaacgccatt atgatggtcg attttgcgct cgaagcccaa
    84181 cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc
    84241 ccgattatga tgactaccct ggcggcgctg tttggcgcgc tgccgctggt gttgtcgggc
    84301 ggtgacggct cggagctgcg gcacctcctg gggatcacca ttgtcggtgg actggtaatg
    84361 agccagcttc tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg
    84421 ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga acttcccgac
    84481 agcacccgct ggcaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc
    84541 accatcgtta acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat
    84601 atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc
    84661 tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact
    84721 ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg
    84781 ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc
    84841 gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt
    84901 ccgctgctcg gtccggcgct cggcggtctg ctggtggagt acgcgtcgtg gcactggatc
    84961 tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgct gttaatgccg
    85021 aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct ggcggttggc
    85081 atggctgtat taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg
    85141 attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga
    85201 aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc
    85261 ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt
    85321 ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg
    85381 gtacttggca gcatgggaat gaagcgaatt gtggttcagg tggtgaatcg ctttggttat
    85441 cgtcgggtac tggtagcgac cacgctgggt ctgtcgctga tcaccctgtt gtttatgact
    85501 accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc
    85561 aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg
    85621 gcgagcagcg gcaacagtct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc
    85681 accatcgccg ggctgttgct gggacttttt ggttcgcagc atgtcagcgt cgacagcggc
    85741 accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg
    85801 gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga
    85861 aaaaggagcg cgcaatgaag ttctggcgac ctggtattac cggcaaactg tttctggcga
    85921 ttttcgccac ctgcattgtt ttgctgatca gtatgcactg ggcggtgcgt atcagttttg
    85981 agcgtggctt tattgattac atcaagcatg gtaatgaaca gcgattacaa ctgttaagtg
    86041 atgcgcttgg cgagcagtat gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc
    86101 gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc
    86161 cgggtatgcc accgcacggc tggcgtaccc agttctgggt ggttgatcaa aacaacaaag
    86221 tgctggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca
    86281 acggtgcgga agttggcgcg gtgatcgcct cccccgttga gcggttaacg cgcaatactg
    86341 atatcaattt cgataaacaa cagcggcaaa ccagctggtt gattgtcgcc ctggcaacgt
    86401 tactcgcggc acttgccact tttctgctgg cgcgcggttt actggcaccg gtaaaacgac
    86461 ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca
    86521 gtgaagatga actgggcaaa ctggcgcaag acttcaacca gcttgccagc acactggaga
    86581 aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat
    86641 tagcggtgct gcgcggtgaa ctggaagcta ttcaggatgg cgtgcgcaaa ttcacgccgg
    86701 agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatctcc
    86761 atcagttgtc gatgtctgat gaaggcgccc tcgcctatca aaaagcaccg gtagatttga
    86821 tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc cgtggcctga
    86881 aactgcaatt ttccctgcca gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc
    86941 agttattcaa taatttactt gaaaacagcc tgcgctacac tgacagcggc ggcagcctga
    87001 aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag
    87061 gtgtcagtga cgagcagcta caaaaattat ttgaacgttt ttatcgcacc gaaggttccc
    87121 gcaaccgcgc cagcggtggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag
    87181 cacataatgg ccgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag
    87241 agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa
    87301 cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta
    87361 tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccgta
    87421 tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg gcaccgatgg
    87481 cttgacgctg tgccgggaaa ttcgtcgttt ttctgacatt cctatcgtga tggtgacggc
    87541 aaaaatcgaa gagatcgacc gcctgctcgg actggagatt ggcgcagatg actacatctg
    87601 caagccgtac agcccacggg aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa
    87661 accgcagcgc gaattgcagc aacaggatgc cgaaagcccg ttgattatcg acgaaggtcg
    87721 ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat ttcgtctgtt
    87781 gaaaacgctc tctcatgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct
    87841 ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta aaaacctgcg
    87901 ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgttgg
    87961 ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatg actttgttag
    88021 ccggagatgg cgttcagacc agatccggca acattatccc acgcatggtc agcaaactg
//
LOCUS       DAJTYZ010000002        77886 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000002 DAJTYZ010000000
VERSION     DAJTYZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 77886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.002"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="OO596_000089"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="OO596_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="OO596_000090"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="OO596_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCR6716068.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="OO596_000091"
     CDS             2354..2758
                     /locus_tag="OO596_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR6716069.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="OO596_000092"
     CDS             2811..2921
                     /locus_tag="OO596_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716070.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3157..3201
                     /locus_tag="OO596_000093"
     CDS             3157..3201
                     /locus_tag="OO596_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716071.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3454..3777
                     /gene="iraM"
                     /locus_tag="OO596_000094"
     CDS             3454..3777
                     /gene="iraM"
                     /locus_tag="OO596_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6716072.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3880..5130)
                     /gene="icd"
                     /locus_tag="OO596_000095"
     CDS             complement(3880..5130)
                     /gene="icd"
                     /locus_tag="OO596_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6716073.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5302..5955
                     /gene="rluE"
                     /locus_tag="OO596_000096"
     CDS             5302..5955
                     /gene="rluE"
                     /locus_tag="OO596_000096"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6716074.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5965..6426
                     /gene="nudJ"
                     /locus_tag="OO596_000097"
     CDS             5965..6426
                     /gene="nudJ"
                     /locus_tag="OO596_000097"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6716075.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6480..7586
                     /gene="mnmA"
                     /locus_tag="OO596_000098"
     CDS             6480..7586
                     /gene="mnmA"
                     /locus_tag="OO596_000098"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6716076.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7622..8263
                     /gene="hflD"
                     /locus_tag="OO596_000099"
     CDS             7622..8263
                     /gene="hflD"
                     /locus_tag="OO596_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6716077.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8267..9637
                     /gene="purB"
                     /locus_tag="OO596_000100"
     CDS             8267..9637
                     /gene="purB"
                     /locus_tag="OO596_000100"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6716078.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9805..10476
                     /gene="phoP"
                     /locus_tag="OO596_000101"
     CDS             9805..10476
                     /gene="phoP"
                     /locus_tag="OO596_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6716079.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10476..11936
                     /gene="phoQ"
                     /locus_tag="OO596_000102"
     CDS             10476..11936
                     /gene="phoQ"
                     /locus_tag="OO596_000102"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6716080.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            12012..13133
                     /gene="roxA"
                     /locus_tag="OO596_000103"
                     /gene_synonym="ycfD"
     CDS             12012..13133
                     /gene="roxA"
                     /locus_tag="OO596_000103"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6716081.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="OO596_000104"
     CDS             complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="OO596_000104"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6716082.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14658..15794
                     /gene="potA"
                     /locus_tag="OO596_000105"
     CDS             14658..15794
                     /gene="potA"
                     /locus_tag="OO596_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6716083.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15778..16635
                     /gene="potB"
                     /locus_tag="OO596_000106"
     CDS             15778..16635
                     /gene="potB"
                     /locus_tag="OO596_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6716084.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16632..17426
                     /gene="potC"
                     /locus_tag="OO596_000107"
     CDS             16632..17426
                     /gene="potC"
                     /locus_tag="OO596_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6716085.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17423..18469
                     /gene="potD"
                     /locus_tag="OO596_000108"
     CDS             17423..18469
                     /gene="potD"
                     /locus_tag="OO596_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6716086.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="OO596_000109"
     CDS             complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="OO596_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6716087.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="OO596_000110"
     CDS             complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="OO596_000110"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6716088.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="OO596_000111"
     CDS             complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="OO596_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6716089.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="OO596_000112"
     CDS             complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="OO596_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6716090.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="OO596_000113"
     CDS             complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="OO596_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6716091.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23801..24874
                     /locus_tag="OO596_000114"
     CDS             23801..24874
                     /locus_tag="OO596_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6716092.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            25002..28448
                     /gene="mfd"
                     /locus_tag="OO596_000115"
     CDS             25002..28448
                     /gene="mfd"
                     /locus_tag="OO596_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6716093.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28592..29548
                     /gene="ldtC"
                     /locus_tag="OO596_000116"
                     /pseudo
     CDS             28592..29548
                     /gene="ldtC"
                     /locus_tag="OO596_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="OO596_000117"
     CDS             complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="OO596_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR6716094.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            30129..30761
                     /gene="comR"
                     /locus_tag="OO596_000118"
     CDS             30129..30761
                     /gene="comR"
                     /locus_tag="OO596_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6716095.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30823..31362)
                     /locus_tag="OO596_000119"
     CDS             complement(30823..31362)
                     /locus_tag="OO596_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6716096.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="OO596_000120"
     CDS             complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="OO596_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6716097.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33301..33843)
                     /locus_tag="OO596_000121"
     CDS             complement(33301..33843)
                     /locus_tag="OO596_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6716098.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="OO596_000122"
     CDS             complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="OO596_000122"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6716099.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="OO596_000123"
     CDS             complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="OO596_000123"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6716100.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="OO596_000124"
     CDS             complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="OO596_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6716101.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36362..36739)
                     /locus_tag="OO596_000125"
     CDS             complement(36362..36739)
                     /locus_tag="OO596_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6716102.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="OO596_000126"
     CDS             complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="OO596_000126"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6716103.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37435..39624
                     /gene="fhuE"
                     /locus_tag="OO596_000127"
                     /pseudo
     CDS             37435..39624
                     /gene="fhuE"
                     /locus_tag="OO596_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="OO596_000128"
     CDS             complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="OO596_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6716104.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41412..42209)
                     /locus_tag="OO596_000129"
     CDS             complement(41412..42209)
                     /locus_tag="OO596_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6716105.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(42220..43224)
                     /gene="holB"
                     /locus_tag="OO596_000130"
     CDS             complement(42220..43224)
                     /gene="holB"
                     /locus_tag="OO596_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6716106.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(43221..43862)
                     /locus_tag="OO596_000131"
     CDS             complement(43221..43862)
                     /locus_tag="OO596_000131"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6716107.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="OO596_000132"
     CDS             complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="OO596_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6716108.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="OO596_000133"
     CDS             complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="OO596_000133"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6716109.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="OO596_000134"
     CDS             complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="OO596_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6716110.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="OO596_000135"
     CDS             complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="OO596_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6716111.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="OO596_000136"
     CDS             complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="OO596_000136"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6716112.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="OO596_000137"
     CDS             complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="OO596_000137"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6716113.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="OO596_000138"
     CDS             complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="OO596_000138"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6716114.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="OO596_000139"
     CDS             complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="OO596_000139"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6716115.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="OO596_000140"
     CDS             complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="OO596_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6716116.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="OO596_000141"
     CDS             complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="OO596_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6716117.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52391..52975
                     /gene="yceF"
                     /locus_tag="OO596_000142"
     CDS             52391..52975
                     /gene="yceF"
                     /locus_tag="OO596_000142"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6716118.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="OO596_000143"
     CDS             complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="OO596_000143"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6716119.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54619..57804
                     /gene="rne"
                     /locus_tag="OO596_000144"
     CDS             54619..57804
                     /gene="rne"
                     /locus_tag="OO596_000144"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6716120.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="OO596_000145"
     CDS             complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="OO596_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6716121.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="OO596_000146"
     CDS             complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="OO596_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR6716122.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="OO596_000147"
     CDS             complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="OO596_000147"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6716123.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="OO596_000148"
     CDS             complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="OO596_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6716124.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="OO596_000149"
     CDS             complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="OO596_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6716125.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="OO596_000150"
                     /pseudo
     CDS             complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="OO596_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="OO596_000151"
     CDS             complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="OO596_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR6716126.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="OO596_000152"
     CDS             complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="OO596_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6716127.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="OO596_000153"
     CDS             complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="OO596_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6716128.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="OO596_000154"
     CDS             complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="OO596_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR6716129.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="OO596_000155"
     CDS             complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="OO596_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6716130.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            68109..68768
                     /gene="flgA"
                     /locus_tag="OO596_000156"
     CDS             68109..68768
                     /gene="flgA"
                     /locus_tag="OO596_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6716131.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68844..69137
                     /gene="flgM"
                     /locus_tag="OO596_000157"
     CDS             68844..69137
                     /gene="flgM"
                     /locus_tag="OO596_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6716132.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            69142..69558
                     /gene="flgN"
                     /locus_tag="OO596_000158"
     CDS             69142..69558
                     /gene="flgN"
                     /locus_tag="OO596_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6716133.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="OO596_000159"
     CDS             complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="OO596_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6716134.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(71243..72166)
                     /locus_tag="OO596_000160"
     CDS             complement(71243..72166)
                     /locus_tag="OO596_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6716135.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(72168..72815)
                     /locus_tag="OO596_000161"
     CDS             complement(72168..72815)
                     /locus_tag="OO596_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6716136.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="OO596_000162"
     CDS             complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="OO596_000162"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6716137.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73646..74854
                     /gene="mdtH"
                     /locus_tag="OO596_000163"
     CDS             73646..74854
                     /gene="mdtH"
                     /locus_tag="OO596_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6716138.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74918..75565
                     /gene="grxB"
                     /locus_tag="OO596_000164"
     CDS             74918..75565
                     /gene="grxB"
                     /locus_tag="OO596_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6716139.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75699..76259
                     /locus_tag="OO596_000165"
     CDS             75699..76259
                     /locus_tag="OO596_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6716140.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76365..77411
                     /gene="pyrC"
                     /locus_tag="OO596_000166"
     CDS             76365..77411
                     /gene="pyrC"
                     /locus_tag="OO596_000166"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6716141.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77485..77730
                     /gene="dinI"
                     /locus_tag="OO596_000167"
     CDS             77485..77730
                     /gene="dinI"
                     /locus_tag="OO596_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6716142.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        19158 a        20494 c        19294 g        18940 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcac ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt tatatgtatt ttagccagga
     3061 acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat gtcatgttta
     3121 tattcattat tatcgttata taaaaaggca accataatgt ttagcaaatt ggcaccaagt
     3181 agcataaagg ctatgtttta attacaggat gttcagtcat ttgaatgtat aacattatag
     3241 ctaaacaaat ctaaaacgaa gtgaataatt tattgttttc actaaatctc attttgttta
     3301 acatccattg agattccttg ctttaaattt tgttttatat aagcaatcgt tttaattaat
     3361 ttattttttg aaggggtaat atactcatat gcaaaataaa gaaatgaaca tccaaatgaa
     3421 ctatattaaa taccgtggga taagacataa caaatgaagt gggtagtaat tgacacggta
     3481 attcaaccta catgcggcat atctttttca gccatatggg gtgatatgaa aaagatcatc
     3541 tggtatcaat ctactatatt tcttcctcct ggaagtatat ttacaccggt taagcttggt
     3601 attatcctta aggaaaaaga atatcctatt actatttata acattgcacc attcaacaag
     3661 aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag caaaataaca
     3721 aataaatgtt tacataatag ttgcattata aaaatatgcc catatgggct caagtaatgg
     3781 gcttcatata aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc
     3841 gtgttatacg cccgtttcaa tatttaactc atggagagat tacatgtttt cgatgatcgc
     3901 ttcaccaaac tctgaacatt tcagcagctt agcgccttcc atcagacgtt caaagtcata
     3961 agttacggtt ttcgcgttga tagcaccttc cataccttta acaattaaat cagccgcttc
     4021 agtccacccc atatggcgca gcatcatctc agcggagagg atgatagagc ctggatttac
     4081 tttgtcctga ccggcatatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc
     4141 gtcaccgatg tttgcgccag gggcgatacc gataccgcca acctgcgctg ccagggcgtc
     4201 agaaatgtag tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag
     4261 caggatctgt tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt
     4321 gttcgggttt ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaactcttc
     4381 acgcgccagc tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc
     4441 tttgtgcacc agagtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg
     4501 aaccagacgt ttggtgcctt cttccgaaca cggcttaata ccgataccac aatgttccgg
     4561 gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc
     4621 ggcagagtct gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat
     4681 aaccatatcg gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac
     4741 cggacgcagg cagatgtaga gatccagttc ctggcgcagg gcaacgttca gagagcgaat
     4801 accgccacca acaggagtgg tcagcggacc tttaatggca acgcgatatt cacgaatcag
     4861 atcaagggtt tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc
     4921 ggtgtaaatt tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc
     4981 gtcgaccact ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta
     5041 agggataatc ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc
     5101 ttgtgccgga acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc
     5161 tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat
     5221 tttttgttaa tgatttgtaa ttggcttgtc aatgctaccc tattgtttgc cgtcatgaaa
     5281 gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa
     5341 acttctttta gaaatcacca ggttaagcga ttcagctcgc aacgttctac taggcgtaaa
     5401 cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac cctacgatgt tcttccgcag
     5461 ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat
     5521 gcagcaggtc gtcttgaccg cgataccgaa gggttactgg tgctgaccaa taacggcgcg
     5581 ttgcaggcgc gtttaaccca gccgggcaaa cgcaccggga aaatctatta tgtgcaggtg
     5641 gaaggtattc ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat
     5701 ggccctaccc tgcccgccgg cgcggagctg gttgacgaac cagcgtggtt atggccgcgg
     5761 aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat
     5821 gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga
     5881 ctgattcgtt atgcgatggg tgattacact ttggataacc ttgccaatgg cgaatggcga
     5941 gaagcaacag aataaggatg cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg
     6001 cacgcagagg gtaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac
     6061 caacctgccg ggcatctgga agccgatgaa accttagtgg aagctgccgc ccgtgagcta
     6121 tgggaagaaa ccggcatcag cgcgcaaccg caacacttta ttcgtatgca tcagtggatt
     6181 gcgccagata aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc
     6241 ccgacccagc ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt
     6301 ttaaaggcgt caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagt
     6361 gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc
     6421 atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa
     6481 tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt
     6541 ctgcctggct gttgcaacaa cagggatatc aggttgaagg cctgtttatg aagaactggg
     6601 aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg
     6661 tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca
     6721 atgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc
     6781 tgtgcaacaa agagatcaaa tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg
     6841 ccgattatat cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc
     6901 tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg
     6961 agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga
     7021 ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca
     7081 ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggggcaa ccgggcaaaa
     7141 tcattaccgt cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg
     7201 gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg caccgaagaa ccttggtatg
     7261 tggtggacaa agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc
     7321 ggctgatgtc tgtcgggttg attgcccagc agttgcactg ggtcgatcgc gaaccattca
     7381 ccggcactat gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg
     7441 tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgaaccggtt gccgccgtga
     7501 cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg
     7561 agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa
     7621 cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg
     7681 cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact
     7741 caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc
     7801 caacctgcgc gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gtcgccaggg
     7861 cttaaacgcc gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc
     7921 ctcagcgaaa ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct
     7981 cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga
     8041 tgtgatcagc ccgcttggcc cacgcattca ggtcaccggt tcccctgctg tactgcaaag
     8101 cccacaagtg caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct
     8161 ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac
     8221 tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc
     8281 actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg
     8341 gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca
     8401 aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg
     8461 ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat
     8521 cgagcgtacc actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc
     8581 ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga
     8641 tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc
     8701 gtactggcgt caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc
     8761 gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc
     8821 aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg
     8881 caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga
     8941 ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac
     9001 tacccagatt gaaccgcacg actacatcgc cgagctgttt gattgcgttg cacgcttcaa
     9061 caccattctg atcgactttg atcgtgacgt ctggggttat atcgccctta accacttcaa
     9121 acagaaaacc attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat
     9181 cgacttcgag aactccgaag ggaatctggg cctttccaac gcggtattac agcatctggc
     9241 aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa
     9301 cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag
     9361 caaactggaa gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct
     9421 ggctgagcca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct
     9481 gaaagagctg actcgtggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg
     9541 tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg caaactacat
     9601 tggtcgcgcc atcaccatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg
     9661 atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct
     9721 ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa
     9781 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     9841 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga tgcagaagat
     9901 gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc
     9961 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca
    10021 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
    10081 gctggtgctg atgattacgt gactaaacca tttcatattg aagaggtgat ggcgcgaatg
    10141 caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct accaccgttt
    10201 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
    10261 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat
    10321 tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
    10381 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt gattactacc
    10441 gttcgcggcc agggctattt gttcgaattg cgctgatgaa aaaattactg cgtctttttt
    10501 tcccgctctc gctgcgggta cgttttctgt tggcaacggc tgcggtagta ctggtgcttt
    10561 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
    10621 ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata
    10681 agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt
    10741 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
    10801 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
    10861 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
    10921 atgatgacga cgcggagatg acccactcgg tggcagtaaa cgtctacccg gcaacatcgc
    10981 ggatgccaaa attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
    11041 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
    11101 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
    11161 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
    11221 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc
    11281 gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
    11341 cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg
    11401 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
    11461 gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
    11521 cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
    11581 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata
    11641 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
    11701 tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt
    11761 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag
    11821 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg
    11881 gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat
    11941 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
    12001 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
    12061 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
    12121 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
    12181 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
    12241 ccaactggtc attactggta caggcagtga accactggca tgagccgacc gccgcgctga
    12301 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
    12361 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
    12421 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
    12481 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
    12541 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
    12601 actattccgt gggctttcgc gcgccaaata cgcgggaact gattagtgga tttgccgatt
    12661 atgtgctgca acgtgaactg ggcggcaact actacagcga tcctgatgtt ccacctcgcg
    12721 ctcatcctgc ggatgttctg cctcaagaga tggataaact gcgtgagatg atgctcgaat
    12781 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
    12841 atgaactgga tatcgcgccg ccagaaccgc cttatcagcc agatgaaatc tacgatgcgc
    12901 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
    12961 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
    13021 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
    13081 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
    13141 tttaagccgg atgcggcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
    13201 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac
    13261 tcatgcttac catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg taatcccata
    13321 aacgacaact gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg
    13381 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctct
    13441 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
    13501 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    13561 tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc
    13621 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    13681 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    13741 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
    13801 agttcgccta cgccaccacc gtcaacagtg taagcccaac gggcatcgaa ggcatcaaca
    13861 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    13921 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    13981 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    14041 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    14101 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    14161 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    14221 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    14281 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    14341 ctcacccctg cttttgattg ggtatccaga gatacgtagt tcaaaaatcg ctcaagtagt
    14401 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    14461 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    14521 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    14581 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    14641 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    14701 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    14761 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    14821 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    14881 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    14941 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    15001 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    15061 tgcaactgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    15121 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    15181 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    15241 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    15301 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    15361 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    15421 acgccactgt catcgaacgt ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    15481 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    15541 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    15601 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    15661 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact
    15721 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    15781 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    15841 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    15901 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    15961 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    16021 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    16081 ctgtttctgc tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa
    16141 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    16201 acaccaattc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    16261 ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    16321 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca
    16381 ctgacgatgc cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    16441 ttctatgtat ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    16501 gtccagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    16561 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag
    16621 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    16681 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    16741 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt
    16801 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacattt gctacgctta
    16861 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta
    16921 gcggaatgct gtttgtggtg atgatgtcac cagatatcgt gatggcgatt tctctgctgg
    16981 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    17041 ccttctgcct gccgtttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    17101 ggatgctgga agcggcgaaa gatctcggtg ccagtgaatt taccattctg cgaaaaatca
    17161 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    17221 tggacgatgt ggtagtttct tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa
    17281 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    17341 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    17401 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    17461 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    17521 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    17581 ggctatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa
    17641 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    17701 agggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    17761 catgctcaac aagccgtttg acccgaataa cgactactcc attccgtata tctggggtgc
    17821 gacggcgatt ggcgttaacg gtgatgcggt ggatcctaaa tctgtcacca gttgggcaga
    17881 tctgtggaag ccagagtaca aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt
    17941 ccagatggcg ctgcgcaaac tgggctactc cggtaacacc accgatccga aagagattga
    18001 agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    18061 cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggctctgc
    18121 tttcgttgca cgccaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat
    18181 tttctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    18241 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta
    18301 tccaacgcca aaccttgcgg cgcgtaagct gttaagcccg gaagtggcga acgataaaac
    18361 gctctacccg gatgctgaaa ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc
    18421 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaaacg
    18481 ggcggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag
    18541 taaagtttta tcccgctctt tcgtcggtat tgtggtcccc ttgtaggcct gataagcgta
    18601 gcgcatcagg caatgcttcc tgctttataa tcccttcagc aacttttcaa caaactcagg
    18661 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccga cctgacttgg
    18721 ttcgagattc agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc
    18781 cgccggataa acatgcccgg aagtaccaat ggcaatgaaa atatcagcca tcgacaacgc
    18841 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa
    18901 gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    18961 gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac
    19021 attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg
    19081 atcgccgagg gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    19141 tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    19201 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    19261 ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac
    19321 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa
    19381 acgctcgcgc aggcggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    19441 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    19501 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    19561 acgaggcagc ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    19621 aatgacgacc aggtcagagt caacgatggt caggatattt cccagacaaa ccgctaataa
    19681 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    19741 aatttcggga gcctgcaacg gttgatgata atagtgttgc cagagccacg caaagccccg
    19801 acctgataga taattttcga tacaaccaat ttgaccacag ccgcagcggc gtaacgggaa
    19861 atccagcccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    19921 aatgtagctt ttaccggtaa tcggtttacc gttgaaaatc agcccgccgc caacgccggt
    19981 gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcatccc aggcttctga
    20041 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    20101 gtcggcacgc agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc
    20161 ttctgtttcc ggcattcccg gaataccgat gccaacagaa cctttacagc caaaacgttg
    20221 gtcagcttcg gctaccagct cgcatactgc atctaaaaat gcgtcatagc tgtcacgcgg
    20281 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    20341 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    20401 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc
    20461 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    20521 gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga
    20581 tcagcttttc aatccgctca ataatcgggg taagttgcag tgaaacaact acgccgataa
    20641 tcacaccaca caggctaccg aacagccctg ccagcaatcc ataccagaca aagatggcgc
    20701 gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt
    20761 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    20821 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    20881 caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    20941 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    21001 caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    21061 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    21121 cgggattcga gttgggaatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    21181 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccaggcat
    21241 ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgcggg ttaacgccct
    21301 tcacctgaat tgcgcgcaga ttggtaccac tttctaccag tccggtgaaa ttgatatacg
    21361 gcgcagcggc ggcaataccc ggcacttttt gcacgttatc cagtgcttcc tgccagttag
    21421 tccatggctg atttaccgcc tcaatttcac cgtgcggtac cacagcaaga atgcggttat
    21481 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    21541 gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc
    21601 cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    21661 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    21721 cgccagttgc aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc gattcaattc
    21781 cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggctcatc
    21841 cgcaagtacc agacgcggat tattgaccag cgcacgggca atcgccacac gctggcgttc
    21901 gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    21961 catctccagc gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat
    22021 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    22081 cagcttctgg ttgcgcagtt ctgctttcgc cgccgaagac agtttgctca tcggttgacc
    22141 gttaaagatc acatcgccgg aggttggtgt atccagcccg cccagcaggt gcagcaaggt
    22201 acttttaccg gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa
    22261 actgacattg tgcagtacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    22321 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttga gtggcggcag
    22381 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    22441 caatgacctg taaaggttcg atagcgaccg gcagcgccgc gccatcaagc aggacgccga
    22501 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga
    22561 tcgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg
    22621 gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatt accatcagcc
    22681 ctagtgaggt aataatgtta aacgccgcaa cggcgacaat caggctcagc agcaagccca
    22741 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    22801 gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactttc agcggctcat
    22861 ccagccacaa acgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat
    22921 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg aaattgccaa
    22981 tcacattgaa caggcgctgg cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    23041 gtaccatcac gcggatttga tcaccgcgat taacgcctag ctgtgaggca agttgttcgc
    23101 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    23161 gcgtaagtgg atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc
    23221 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    23281 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    23341 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    23401 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    23461 agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata
    23521 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    23581 gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    23641 cccccgtaaa tcaatcggct aaattgccat gttttatcat caatcttgcc gcctgatctt
    23701 aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca taacccgcag
    23761 gatcaaccgc atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg
    23821 gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac
    23881 cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga gtaaatgctg
    23941 gatctatttc aatctttacc ttgcaccctt tcgtatgccg gtttttttct ttatatccgg
    24001 ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat
    24061 ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc
    24121 gctgaaccag tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc
    24181 cgattccacc ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct
    24241 gtatgcgtta attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgccact
    24301 attcgccttg tttgtactgc tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg
    24361 aatgaacagt gtgatccgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac
    24421 aataatgacc tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct
    24481 taccgttctg gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc
    24541 gattgtggtg atcatgaagc tgttttatca gtacgagcaa cgattcggta tgcgctccac
    24601 cagcctgctg aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct
    24661 ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca
    24721 agtcgaactg acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc
    24781 gggcttgctg gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc
    24841 ttcactgccc gctgccgtca gttactctcg ttaaaccatc gccccagccg gggcgattca
    24901 atttgctaaa ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc
    24961 catatgttga ggcatatcct aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt
    25021 atacactgcc cgtcaaagcg ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct
    25081 gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg tccggtggta ctcattgcac
    25141 cagatatgca aaatgctctg cgtttgcatg atgaaatcag ccagttcacc gatcagatgg
    25201 tgatgaatct ggcggactgg gaaactcttc cctacgacag tttttcgcct catcaggaca
    25261 ttatctcctc gcgcctttcc accctttacc agctaccgac gatgcagcgt ggcgtactga
    25321 ttgttccggt gaatacgctt atgcagcgcg tttgcccaca cagttttctc cacggtcatg
    25381 cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc attacgaacc caactggaca
    25441 gcgccggtta tcgccatgtt gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg
    25501 cgttgctgga tctcttcccg atggggagtg agctgcctta tcgtcttgat ttctttgatg
    25561 atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag
    25621 aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga taaagcggca attgaactgt
    25681 tccgcagcca gtggcgcgat accttcgaag tgaagcgcga tccggaacat atttaccagc
    25741 aagtgagtaa aggcacatta cctgccggga tcgagtactg gcagccattg ttcttcagcg
    25801 aaccactgcc gccgctgttc agttatttcc ctgccaatac cttgctcgtg aatactggcg
    25861 atctggaaac cagtgccgaa cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg
    25921 gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct ctggctgcgg gtggacgagc
    25981 tcttctcaga gctgaaaaac tggccccggg tgcagctaaa aactgaacat ttaccgacaa
    26041 aagccgcgaa tgccaattta ggtttccaga aactgccaga cctggccgtt caggcacaac
    26101 aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt
    26161 tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga actgctcgca cgaattaaaa
    26221 ttgctccgca acgcattatg cgtcttgatg aagccagcga ccgtgggcgt tatctgatga
    26281 ttggcgctgc cgaacatggt tttgtcgata aggtgcgtaa tctggcgctg atctgcgaaa
    26341 gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc
    26401 ccgatacact gatccgtaac cttgcggagc tgcatattgg tcagccggtg gtccatctgg
    26461 agcacggtgt cgggcgctat gccggaatga ccacgctcga agcgggcggc attactggcg
    26521 agtatttgat gctcacctat gccaacgacg ccaaactgta tgttccggtg tcgtcactgc
    26581 atctgattag ccgttacgca ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg
    26641 gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg
    26701 aattgctgga tatctacgcg caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc
    26761 gtgagcagta tcagttgttc tgcgacagct tcccgtttga aaccacgccg gatcaggcgc
    26821 aggccattaa tgcggtactt agcgacatgt gtcagccgct ggcaatggat cgtctggtgt
    26881 gcggcgatgt tggctttggt aaaacagaag tggcgatgcg cgccgctttc ctggcagtag
    26941 ataaccacaa gcaggtggcg gtgttggtgc ctaccaccct tctcgcgcag cagcattacg
    27001 acaacttccg cgaccgtttc gccaactggc cggtacgtat cgaaatgatc tcccgtttcc
    27061 gcagcgccaa agagcagacg caaatccttg cggaagtggc ggaagggaaa atcgatattc
    27121 tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt taaagattta ggcctgctga
    27181 ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg
    27241 cgaacgtgga tattctgacg cttactgcaa cgccgatccc acgcacgctg aatatggcaa
    27301 tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta
    27361 aaacctttgt ccgtgagtat gacagcctgg tggtccggga ggcgatcctg cgtgaaattt
    27421 tgcgcggggg gcaggtttat tatctctaca atgatgtgga aaacatccag aaagctgccg
    27481 aacggctggc agaactggtg ccagaagcgc ggatcgccat cggtcacggg cagatgcgcg
    27541 agcgcgaact ggaacgggtg atgaatgatt tccatcatca acgtttcaac gtgctggttt
    27601 gtacaaccat tatcgaaacc gggatcgaca tcccgacagc caacaccatt atcattgaac
    27661 gcgcggatca cttcggtctg gcgcagctgc accagttacg cggtcgcgtc ggacgttcgc
    27721 atcatcaggc atatgcatgg ttgctgacac cgcatccaaa agcgatgact accgatgcac
    27781 aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa
    27841 cgcacgatct ggagattcgc ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa
    27901 tggaaaccat cggtttctcg ctgtatatgg agttgctgga aaacgccgtc gatgcactga
    27961 aagccggacg cgagccgtcg ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc
    28021 ggatgccgtc gctattgcca gatgatttca ttcctgacgt gaatacgcgt ttgtcgttct
    28081 ataaacgtat tgccagcgcc aaaacggaaa acgaactgga agagatcaaa gtcgagctta
    28141 tcgatcgctt cggcctgctg ccagatccgg cgcgtaccct gctggatatt gcccgtctgc
    28201 gccagcaagc gcagaaactg gggatcagga agctggaagg taatgagaaa ggcggggtga
    28261 tcgaatttgc cgagaagaat cacgttaatc cggcctggtt gattggtttg ctgcaaaaac
    28321 agccgcagca ttaccgcctt gatggtccga cgcgcctgaa atttattcag gatttgagtg
    28381 agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg tgaactggaa gagaacgcga
    28441 tcgcttaatg gtaaatcggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt
    28501 tgcacttccc tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt
    28561 acaataacct tatatttatt attgattttt ggtgatgatc aaaacgcgtt tttctcgctg
    28621 gctaacgttt tttacgttcg ccgctgccgt ggcgctaccg gcaaaagcca acacctagcc
    28681 gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga
    28741 cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca
    28801 ggctaacccc ggcgttgatc cttacgtacc gcgcgcgggc agcgtgttaa cgatcccgtt
    28861 gcaaacccta cttccagacg cgccgcgcga aggcattgtg atcaacattg cggagctgcg
    28921 tctctattac tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag gtattggtca
    28981 gttaggtggt gacacgctga caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa
    29041 cccaacctgg acgccaacgg caaacatccg cgcccgttat aaagcacagg gaattgagtt
    29101 gcctacggta gtgccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc
    29161 ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg
    29221 ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt
    29281 caccccaggc accaaagtga atatcatcaa cactccgata aaagtctctg ccgaaccaaa
    29341 cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca
    29401 gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga
    29461 cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg
    29521 tcatcaagtg agcccacaaa ctctgtaaat cctggataaa aaaaatcccc gcgacacggc
    29581 atgtcgcggg gaaaaaatca gcagtagcag gcattaatga gggttaatgc ttatttataa
    29641 attactgctg ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt
    29701 gcgcccatct catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc
    29761 gcgttagcac tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca
    29821 gccgcaaagt tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg
    29881 tttttcataa tagtggcctt atgcagatga atgacgtcgc aagaaattac ttaacgatcg
    29941 ataactaaat gatagatgtg atctggatca catacaagat attttttata acaatcatta
    30001 attaatttaa aaatctttaa atttcagtta aatagaaagc aattattttt atgggatctg
    30061 gactggtgaa cggaggtgat tatttttata ataacctttc attcacagtc accaggttca
    30121 acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag
    30181 tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg
    30241 aagcgacttc tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg
    30301 cggaatttac caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt
    30361 ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg
    30421 ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct
    30481 tcatgatcaa caactgcacc accctctccc cagattcagg agatatcgcc aatacgttga
    30541 aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc
    30601 gcggggaaat cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta
    30661 ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg
    30721 ccggaacgac tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc
    30781 agcctgagcg aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa
    30841 aatcagttgc ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat
    30901 tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt
    30961 gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga
    31021 gccctggatc tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc
    31081 tttaccacga ccaccaccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac
    31141 cgacccggta atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttgcg
    31201 acactcctga cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc
    31261 gtattgcggg ccccgttcaa acacgttcag actggccact gccgctacgc ccagcgcagc
    31321 tgcgacacca atcccgatac ccgccaacat tgatttattc acgggaacac ctccttcttt
    31381 gcagttatgc caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag
    31441 cgcgggaatg gctggaaata cttaaggatt cggagaagtc tgaaggaatg cctgatgcga
    31501 cgcttaacgc gtcttatcag gcctaccgct cgtaccattt gtccagggcg gatgaggcgt
    31561 ttatgccaca tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc
    31621 ggttaatact ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct
    31681 gatgcattcg gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg
    31741 agccgcgcgt caggttaccc atcaggctac cgacagtgga gaagttcgac agcgatacca
    31801 gcgaaccatg atctttatac tgataatttt tcagcggttt accgttcatc tgcgccagaa
    31861 tatttttcat tgcgcaggtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc
    31921 ccccttccgg acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg
    31981 tggtttgcag cgtcggttcc accaccagct ggttgatacg gttggtttca agaccaccga
    32041 tatctttcag gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa
    32101 tatattcacc atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg
    32161 tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg
    32221 gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg
    32281 tcaggccttt gtaaccgtag ctgtgcagtt gcttgaccgc attgtacaat tcagcggaga
    32341 gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca
    32401 ggttggcgga gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt
    32461 gcgggttatc gaggaaaatg cagttctctt tgacacctgg cgtattgaaa tcgttagagg
    32521 tgctacccag cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt
    32581 cacctttctc atcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa
    32641 tgacggaacc cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg
    32701 catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacagat
    32761 ggctgtggtt acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc
    32821 ccagctgtgt tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca
    32881 atggcgtagt caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttataa
    32941 acatagctct taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata
    33001 acatgaatgg tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag
    33061 ataggcgact ttaccgggtg agatcaaaaa acagagttta ttgtttatta tttgagcaag
    33121 tccggaaggg tgatataggg tgacgcgtat atcgagggcg tttggtcgtc ctgataagat
    33181 gcgcagcatc gcatcaggca ttgatgctcg actgtcggat gcggcgtaaa cgccttatcc
    33241 gacttacaga attcggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat
    33301 ttacccgagg gttttgaacg ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt
    33361 gtgcgtctgc tcttcgtccc agacaatctc gtaataatga tgcaactctt cagatgtccg
    33421 ctggctgtta agcgcttcat cattacgcga caaaatcacc aggcaacgat cgcgattctt
    33481 ctcgcggaag ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat
    33541 cttgccttcc atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc
    33601 gcaaagaaaa ccaatccgtt ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg
    33661 acgctcgtcg acgttcagtt gcaacatttt gtccacttct ttgagcagat gctgcatatc
    33721 atgtttcgga tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa
    33781 ttgtaagact ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat
    33841 catcgcttcc tcacataagc cagggttagt gacctgcttt ctcttcctgc cagcgttcat
    33901 gtaactgatt cagacgggtg ctgatcgcct tccagcgagc cgagtccatc agttcctgtc
    33961 gcgaaaatga acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat
    34021 tatctaacac gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacctg
    34081 cgtccagtga tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg
    34141 ataaatcgtc agagaaaatt acaccgtcaa aacacagttc ctgacgcaaa acggttttca
    34201 gccagtagga agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag
    34261 gcataatggc gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag
    34321 cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta
    34381 ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat
    34441 caataaaccg gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc
    34501 caatcgccgc gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca
    34561 tagcgatcat ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt
    34621 ccattcccaa cagcgcagca aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa
    34681 aacgctgcac gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgatgctg
    34741 cgcggatctg gcgcaccagt tcacgtaact gggcaggatc atgatagtta cgcgtaaaga
    34801 gaatcagccc tcccaccagc ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt
    34861 aaccttcgac atccaacatt actggaccca cactgctctc cttattgttt tattaatagc
    34921 tgccgccagg tgtcatcggc cagcctgata aattgttgat cgccgatttg tcgccagcgg
    34981 tactcaaacc accctgcttt gagcatcagc agccagggaa accatcgcct gacctgacgc
    35041 cataattgcg ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc
    35101 tgttcagtat tttccaccca caccgccgcc agttccagcg cgatatcacc atctccggca
    35161 tactcccagt cgatgagttt taaccctgac gcgctatgca ctaaatttcc ggcgtggaca
    35221 tccatatgca atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt
    35281 aacattcgca gccaacccac agtccgccgc gccggatcgc tttgctgcca gtacagttcc
    35341 agtaagggca acagcgttat tcgccagcca aaacgtggtt gttgatgaag ataatacagc
    35401 aagcctgcca gttcgttggt atccggcaaa tacgttttta ccgcgccggg cagatagtcg
    35461 actaccatcc agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt
    35521 gataaagccc gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga
    35581 cgcacaacaa aacgctggcc ctgatgttca atgagaaaac taccgccact aagcccacta
    35641 ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg
    35701 atgggattat tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac
    35761 cagcatcagc tgcatttgta gggtcggagc gttaacgttg ccagaggcgc tggagtacag
    35821 cacgtaatga gcgccgacat tgcgggcaat gcctatggct ttactacggg tacctaaact
    35881 gtcctgcggc gacaaaccta actgttgctt agccatcgac agctgctggg cggaaaccag
    35941 ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt
    36001 cagcgaaccg ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac
    36061 cccgtcagcc ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg
    36121 gcgaatatgc ggcgcaggcg gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg
    36181 cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc
    36241 cggcgctggt ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag
    36301 aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc
    36361 cttacaaata tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca
    36421 gcgttaccgc cgaatgtgcg ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct
    36481 ccagccctct ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt
    36541 cgttataaag cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt
    36601 ctgcactgat gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca
    36661 ccggaatttc cggatgtgaa cgacagccca ccagcagtaa caacgccaga gacaccagcc
    36721 caaagcatcc ttttctcatc gttacagacc tttatgcgcc agcattggtc ccagcggacg
    36781 gccacccaac aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtatt
    36841 catgatcaga cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac
    36901 ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac acgtcgttca cagtcggaat
    36961 gaggatattc ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc
    37021 cgttaccaga tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct
    37081 gaatatagtt tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg
    37141 ctacgccgcg ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaacct
    37201 taactcacga tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc cattattccc
    37261 atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg acaaatatga
    37321 atgcgcatat ttctcatttg catttacaaa caaaattatt cgcacactaa atataaccgc
    37381 tcatcaccct cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt
    37441 tcaacacaat ttaacaggga taatcaatat caagccatca ccaaaccgtc actacttgcc
    37501 ggttgcatag cactggcact attaccttct gccgcttttg ctgcaccagc cactgaagaa
    37561 acggtgattg ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta
    37621 acgtctacct ctgcgggtac caaaatgcag atgactcaac ttgatattcc tcagtcggtc
    37681 actattgtta gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg
    37741 gaaaacacgc tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc
    37801 cgtggattcc agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc
    37861 tggaatctgg gcgacgcact ttctgatatg gcactctttg aacgcgtaga agtagtgcgt
    37921 ggcgcgacag gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga
    37981 aaacacgcga ccagtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac
    38041 aaagaacggt atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg
    38101 cgaattgtcg gcggctacca gaataacgac tcatggctgg accgctacaa cagtgaaaag
    38161 accttcttct cgggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt
    38221 tacgaatatc agcgcattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat
    38281 actgatggca gcagcaacag ttacgatcgt gcacgcagta ccgcacctga ctgggcgtac
    38341 aacgataaag agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctag
    38401 caagcgacac tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc
    38461 gatgcctatg taaacaaagc ggatggtatg ctggttgggc catacagtaa ttatggacct
    38521 ggctttgatt atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg
    38581 gatttgttcg ctgacggtag ttatgaattg tttggtcgtc agcacaatct aatgtttggt
    38641 ggcagttaca gcaaacaaaa caatcgttac ttcagttcat gggccaacat cttcccggat
    38701 gaaattggca gtttctacaa ctttaatggc aatttcccac aaaccgactg gtcaccacag
    38761 agcctggcac aggacgatac cacacatatg aaatcgttat atgctgctac tcgagtcacc
    38821 cttgccgatc cgctgcatct gatcctcggc gcacgttata ccaactggcg ggttgatacg
    38881 ctgacttaca gcatggagaa aaaccacacc acgccttacg ctggtctggt gtttgacatc
    38941 aatgacaact ggtcgaccta cgccagctat acctctattt tccagccgca aaatgatcgt
    39001 gacagttcag gcaaatatct ggctccaatc accggtaaca actacgagct gggtctgaaa
    39061 tcggactgga tgaatagccg tctgaccacc acgttagcca tcttccgtat tgagcaggat
    39121 aatgtcgctc agtccaccgg tacacctatc cccggcagca acggcgaaac cgcctataaa
    39181 gcggtggatg ggacagtcag taaaggggtg gaatttgaac tcaacggcgc aattaccgac
    39241 aactggcagc tgacatttgg cgcaacgcgc tatattgcag aggataacga aggaaacgcc
    39301 gttaatccta atctgccacg caccacggtt aaaatgttca ccagctatcg gttgcctgtc
    39361 atgccagagt tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta taccgacacc
    39421 gtgacaccgt atggcacctt ccgcgccgag caaggtagct acgcgctggt ggatctcttc
    39481 acccgctacc aggtgacgaa aaacttctcg ttacagggga acgtcaataa cctgttcgac
    39541 aaaacctacg ataccaacat ggaaggttct atcgtctacg gcgcaccgcg taatttcagc
    39601 attaccggca cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat ctggcggcct
    39661 tagtctcccc aacgtcttac ggattagtgg ttacggatgt actcatccat ctcggttttc
    39721 aggttatcgg atttagtacc gaaaatcgcc tgaacaccag aaccagcaac cactacgccc
    39781 gctgcgccca gtttcttcag gccagcctga tccactttag acacatcagc aacgctgacg
    39841 cgcagacggg taatacatgc gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca
    39901 accagagccg gtgccatttc gctggtacct gttgcttttg catcttcagt cgcgtcttca
    39961 cgacccggcg ttttcagatc cagtgcttta atcagcacgc ggaagatggt gtagtaaaca
    40021 atcgcataac cgataccgac gatcgggaac agccacagtt tgctgctgtt accagacaga
    40081 acgataaagt cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc cagaagaata
    40141 cagattggga atgccaggcc tgccagaatc gcgtggatga tgtacaggat cggcgcaacg
    40201 aacatgaagg agaactcgat cggctcggtg ataccggtca ggaacgaggt cagcgccgcg
    40261 gagatcataa taccgcccac tttcgcgcgg ttttctggtt tagcagagtg ccagatagca
    40321 attgcggcag ctggcagacc gtacattttg aacaggaagc caccagacag tttacccgca
    40381 gtcgggtcac ccgccatata acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg
    40441 gtgtattcac caatctgcat ctggaaaggt acgttccaga tgtggtgcag accaaacggc
    40501 accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca
    40561 gcccactgag agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca
    40621 acgccagtaa agatggcagc caggccagaa atgatcggca caaagcgttt accggcaaag
    40681 aagccaagat actcaggcag cttaatacgg tagaaacggt taaacatgta cgctgcgatc
    40741 gcaccggaga taatccctcc gagtacgcca gtatccgcca ggtgtttaga ggcgatttct
    40801 tcagcaggta aatgcagtac cagtggcgca accacggcca tggttttaac catgatgcca
    40861 taggcaacaa ctgcggccag cgcggatacg ccatcgttat tggtaaagcc gagggcgaca
    40921 ccgatcgcaa aaatcagtgg catgtttgca aagacggaac cgcctgcttc tgccataaca
    40981 tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag cagaatacct
    41041 gcgataggca gtacggatac cggcagcatc agcgatttac cgaccttttg caggttagca
    41101 aatgcattct taaacataat tgagagtgct cctgagtatg ggtgcttttt ttacgttctc
    41161 acgcgtggca aggggggaga gcctcgccgt gtacagggca tctaagcgcc ctttatttat
    41221 tacacagagt aaaataattc agtgccgatt tgtttgacgg ctatcacatt tcaacttcag
    41281 atcattggag aagttgcaca ttttgacaaa acatatgtca caatatttca aacgtggtta
    41341 ttcaccgccc aattttcggc ggtgaacttt actcgtttta gccattaatt acgagcttta
    41401 aaaaaactca ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg
    41461 aagttatcgg tggttacctg cgccagttct tcaacggcaa cacctttcaa cacagccatg
    41521 tattctgcaa cgtcacgaac catcgcaggt tgattctctt ttcctcgatg cggtaccggc
    41581 gcaaggtaag gtgagtcagt ttccaccagt aaccgatcca gggggacata acgcgcagca
    41641 tctcgaagtt gctccgcatt acggaaggtc acaatgccgg aaaaggagat gtaaaatccg
    41701 agatccagta atttacccgc cgtttctctg tcctctgtaa aacagtgtag tacgccaccg
    41761 caatccgtca ctttttcttc gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta
    41821 tggacgataa ccggcttgtt cagttcacga ccaatctgga tatgatggat gaaggactct
    41881 tgctgacgaa ctttagtttc cggtgtgtaa taataatcca gcccggtttc acccagcgct
    41941 acaacaccct cttctgccgc cagacggcgt aaatcttcta catcgtaggg atcattctgg
    42001 ttgagtggat gcacgccaca agaaaatacg acgttgtcac gttcgcctac cagatcccgc
    42061 atatgtaaat aacccggtaa tgttgtggcg actgccagac aaaatttcac atcgcgtgcg
    42121 gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag attcataatc cagaccatcg
    42181 agatggcagt gtgagtcgac taaaaacatg atgtctctct taaagatgag gaaccggtag
    42241 cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa agatcggtga tgagaagctc
    42301 gcggttgatg cctgtgacag acattaactg ttcacgaatg tggcaaacat cccccagtat
    42361 agcctgcagg cgcgagggag aaagatggtt tgccagttcg acgacaaggc ccggcacatc
    42421 aacattggtc acctgcgcag caccatgatg gcgttttagc gcatccatca gcaacgttgc
    42481 cagccagtgt aaacgcgccg gagcttgttc atgattaagg gccgccagca gcgaatacca
    42541 gtcgcccgat ggcacgctat atgccaatgc ctgacacaat gtttcacgag cctgccagtt
    42601 atctccctga aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc
    42661 aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc
    42721 cggcggcggc gcaaggtaat gtaaccgaca acgactacgt aatgttgcca gtaaacgttc
    42781 aggctcgcgg gtagccagga aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag
    42841 caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga caactttcgc
    42901 accacctagg cgtgcgtgct cattcagctt ttcggtgacc tcacgtactg catcaatgcc
    42961 cagcgtattt ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg
    43021 catcaactga catccacgac agtgaccgca acttttgtgg ccctgcggtt gttggcagag
    43081 taaataacgg ctcagggcgt agattaaagc atcatcgccc atgcccggta acgcctgaat
    43141 gagtagcgca tggtgacctc ttccggcctg atagctggct accagttttt cgaaatcagg
    43201 tcgtaaccat ggataccatc tcatgcgtcc aactccttca cccagtgggt cacggtagtg
    43261 cggattgcat ccatcacggc ctccagcggc tgggtggcat caatggtatg aatactttta
    43321 tcttgtgcag ccagttccag atagcgggcg cgggtgcgat taaagaaatc gaaagactct
    43381 tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta
    43441 acatcgagat agagcgttaa gtcggggcga aaatccccga gaacagcatc acgcagtgtt
    43501 gccagcatat gttggtcaat accacgtcct ccgccctgat acgcctgagt ggagagatcg
    43561 tggcgatcgc caatcaccca ggtgccgtta gccagagctg gtttgatgac cgtttctacc
    43621 agttgaacgc gcgcggcata aaacatcaga acttcggctt tatcggtaat gacttcatcg
    43681 cctaccgatt tgatatccag caccaggctt cttaactttt cggcaagttg cgtaccgcca
    43741 ggttcccgag tgaaaaccat gtcgcggata cccagttgct caagcgtctc aaccaccaca
    43801 ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa tgacgatata cttactgcgc
    43861 atttttttcc ttaagcactt tcagataatc ctgcacagac ttgttatggc tggcaagatt
    43921 ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa tagagatacg gcgtttttgc
    43981 cggatgcgca gcagccttca gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa
    44041 accggtaatg gtataggtgt tatacgctgt cggcgtttcc aggtctgcac gagaaagttt
    44101 gccattataa cgctctccca tcccgtaaat cacggttggg tcggtctgca ggcgcatacc
    44161 aatgcgtaaa cggttgataa ataccgaggc aacctgatcg cgttcactgg caacggcggt
    44221 ttctttttcg ataattgatg ccatcgtcac cagctgattt ttatctttat aaggcagacc
    44281 gtccgcacgc ccttcccagg cgctatcgac cgctttcacc attttcttgt gcgctcgctt
    44341 gagtaacgcg acatcggtgg tattggcggt atacatccag gtgtctggcc agaaccaacc
    44401 ttcaatccac tccgggtttt ccagttcaag tgcctgcgct acggtggcgt acttatcgtc
    44461 actcagcgtg tgcttgatat acggagcctc acgcaattgc ttgaggtagt cgctcagacg
    44521 catcccttct accagtcgca gcgggaactg tgcttcttta ccgctttcca gcaatttcag
    44581 catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag
    44641 atccggttcg atacgcagca accattgaaa aacccgtggg cgattgatga tcttatcggc
    44701 ataaagctgt tcaccaagcg ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc
    44761 ttctttgata agcaatttgc tgtcggcaag atggcgaacc ttccagacgc ccacaccagc
    44821 ggcgataccc agtaccacca gcaataacaa gataatcaat aacacttttt tcatgactaa
    44881 ttcgggcgct cacaaagtgg ggctaaatat tcatataacg ttgctgacga aaaggagaca
    44941 tcgccacagg cacgtacggg catcactggc attaacgcat tacaaataac catctcatct
    45001 gcctgcaacg cctcttccag agaggcttgc acttcgacaa gctgataaga ggattgtgcc
    45061 agcaaacgga tacagaattg tcgcataatg ccgttaacac ctgcctgatc cagacgcggc
    45121 gtataaacta cattgccctt ccgccagaac aaattagccg cacagcattc cgtaacccac
    45181 ccttcgctgt caaggaccag cgcctcatca gcgtttgtct gctcaagatg agagcggatc
    45241 aatacttgct caagccgatt aagatgttta atacctgcaa gatgaggatt gcgccccagc
    45301 cgcaccgggc ttagcgccaa cgtcatccct tcgttacgca aacggtcgta atgtgcagga
    45361 taaaccgtaa cggagagaat ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc
    45421 ccgccactac cgcgactgat cacgactttc agcacaccat tttgctgttc tgctgccagc
    45481 gttttcatct cttgttcaag ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa
    45541 gcatcctgta gtcgctggat atgcgccgat aacaaactga ctttaccgtc gataactctg
    45601 gcggtggtaa aacaaccatc accaaactgc gttgcccgat cgcttactgc cagcgattcc
    45661 tgcttatgac cgttaattaa gaacataccg gctccttatc agagaattgt tagtgtggca
    45721 gcatgttcac tacggaacaa gtcggaataa aagctaagaa aaaaggcccg caagcggacc
    45781 ttttatatgg gtgggaaatg acaacttaga tctttttaaa gatcaaagaa ccattagtgc
    45841 caccgaagcc gaaggagtta cacagcgtgt attccattcc gctgacctga cgtgcttcat
    45901 gcggtacgaa atccagatcg caaccttcat ccgggttatc caggttgatg gttggcggaa
    45961 cagcctgatc gcgcagcgcc aggatggagt agatagattc tactgcaccc gccgcaccta
    46021 acaggtgacc ggtcatagat ttcgtggagc ttaccaacac acggcttgca gcttcaccga
    46081 agatggtttt caccgcctgc gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg
    46141 cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc acgcagagca tttgccatcg
    46201 ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat atgataagca tcgctgctca
    46261 taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact
    46321 cttcaagtac cagcataccg gcaccatcgc ccagtacgaa gccatcacgc tctttatccc
    46381 acgggcggct cgccgcttgc gggttatcat tgcgggtaga taatgcacgt gccgcgccaa
    46441 aaccaccaac acccagcggc gtactggctt tctctgcgcc acctgcaacc atcacgtcag
    46501 catcgccata cgcgataata cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg
    46561 cggtcgcgat agagatgctt gggccacgca ggccatacat gatagtcaga tgacctgcca
    46621 ccatgttcac aatcgttgac ggaacgaaga atgggctgat cttacgtgga ccaccgttca
    46681 tcagagatgt gtggttttct tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg
    46741 caccaatgcg ggttgcgttc tcttccgtta tatcaaggcc agaatcctgc atggcctgaa
    46801 cgccagcgac aattccatat tgaatgaagg catccatctt gcgctgttct ttgcgcgaga
    46861 taatgtcctc acagttaaaa tcctttacta agccagcaaa tttcgttgca taggcgctag
    46921 tatcgaaatg gtcgattagg ctgatgccac tctgaccggc aagcagagct ttccaggtag
    46981 actctacggt attgccgaca ggagacaaca tgcccagtcc ggtcacaact acacgacgct
    47041 tagacacgtt tgtcctccag ggagggaaaa aatgattcta gtgggacaaa aagataaaac
    47101 tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat
    47161 caatggcagc ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa
    47221 actcttcttc cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt
    47281 caacgaaaga agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa
    47341 ttttcttaac gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt
    47401 tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt
    47461 cttaccacga ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca
    47521 ctaattatta ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg
    47581 gtcagaccat gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt
    47641 cgtcggatgc caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg
    47701 caggaacctg cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg
    47761 tttcaataaa gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg
    47821 ccagtgattt actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc
    47881 catttcccat ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca
    47941 tcatagcgcg cattaccgct tttgacagac ggaaaacaga tgaaaggttg gtttcgataa
    48001 tatcgttcca ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat
    48061 tattgaccag gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga
    48121 tagatgccgg gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcgc
    48181 tgatcgcctg agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg
    48241 cgagcgtttc agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt
    48301 ttccttcaaa attcatgatt ttcctctttt aaagctcaag cgccgctgcc atcgctgaag
    48361 gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca
    48421 ccttgcccgg gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag
    48481 acttcgtcca ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac
    48541 cattggtttc gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg
    48601 tgattttcgc taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag
    48661 acggtacgct cactggtaac ggcagagcac gttttgcgcc cgcagcttta caggcagcgc
    48721 cagcacgctc aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt
    48781 ttaccggaga aacgacctga cctgctgcag cttcttcaca cgctttcgca atagacgcat
    48841 catccagacc gatgattgcc gccatagcgc ccgtaccttc cggtacggct tcttgcatga
    48901 gcttgccgcg catctcaacc agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa
    48961 ccagcgcgga atattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct
    49021 gctgctgcca tacgcggtac agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc
    49081 aggttttatt cagttcttca gctggcccct gctgggtcag cgcccacagg tcgtagccca
    49141 gcgccgcaga agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag
    49201 ccagcattcc aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt
    49261 tttaatcctt atcctagaaa cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg
    49321 cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg
    49381 cgcacgggac agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt
    49441 ccatagacat accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg
    49501 gaaccagcca gtccagttga gaacggtcaa gattatttgc cgccagcgtc tcatcaacga
    49561 tgtgcgccag ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa
    49621 ttgaattctc tggattcaca cggtcagcat taggcagcgt cagcaactca ccatagctac
    49681 cgtcggcatg cagatgggtg gagatgattc ccggctcttc agaggcagcc agcaccgcag
    49741 cgcccgcgcc atcgccaaaa ataataatag tcccacgatc ggttggatcg caggtgcgcg
    49801 ccagtacatc ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat
    49861 cggctacgct taatgcatag gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc
    49921 aacctttaat gcccagcatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag
    49981 cagaagtcgt tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa
    50041 ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt
    50101 ggcgttcgcg gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt
    50161 ccaaatcggc gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg
    50221 tatacatgta cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg
    50281 ctggcagcgt cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca
    50341 gctgggtata cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc
    50401 tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt
    50461 atcaccgtgc cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga
    50521 ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac
    50581 cgttttttcc cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc
    50641 atcgtcttta atgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca
    50701 gttaacaact cattggcttc aagatagccg atataattga tagaagggat tgttttaagc
    50761 accgctgagg catcccgaat actgtcgaga ccctttactt cttcttcacc aatattgagc
    50821 aacgccacgc gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg
    50881 gcaaattgca ccaacattgt actgtcacaa tcgacgttgg cccctaagtc aaggaccacc
    50941 gttttgccct tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc
    51001 aggggcttga gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca
    51061 caggcttgcg ctcgaccttc tttcaccagc tccagggcca cgcgcattga actcccacga
    51121 ctggcgcgga tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata
    51181 atctgcagac gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg
    51241 ggattgccga ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca
    51301 ggcactgtca cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt
    51361 gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac
    51421 taagcttcat cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac
    51481 cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc
    51541 tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt
    51601 tattctgttg tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg
    51661 ggagcaccaa ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgca
    51721 cttcttcagg cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat
    51781 catgcaccgg aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat
    51841 cgatttcacc gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct
    51901 gttcgtctga acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct
    51961 tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga
    52021 gacgttggtt atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta
    52081 cggattcggc gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt
    52141 tttgagccgt acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg
    52201 catattaact ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc
    52261 caataattcg cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca
    52321 tcaacctgca aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat
    52381 ggaaaaaaat atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct
    52441 ggaaaaactg caaatctctt ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag
    52501 cgacgaatca ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc aatctctggc
    52561 gtcacgttat ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga
    52621 aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg caattacgca aagccagcgg
    52681 caatatcgtc accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca
    52741 aacagaagtg gagccttttg acgtccattt ccgtcacctg agcgaggcgg agattgataa
    52801 ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg
    52861 cattacgctg tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct
    52921 aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt
    52981 tgcgctggat gcggttacgt cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa
    53041 acgccttatc aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac
    53101 gcagcttttg caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct
    53161 caccggtccc cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc
    53221 ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg
    53281 caatcggatg acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg
    53341 ggctacaacg caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg
    53401 acggtttgcc ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca
    53461 ataacggcgc ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt
    53521 aatctttttg cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt
    53581 ttttcgctat cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga
    53641 accgcgcttc cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc
    53701 cgccatgtac cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata
    53761 agatgacgtc cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc
    53821 gctcagcaac gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag
    53881 gcttaatacg ttttttgttc acccgcactt cgcctttacg caaaatacgg taaatcatac
    53941 tttttggtac gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt
    54001 cagcggtgat agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta
    54061 attagccaac aggattcgcg ccactcattt ttctatgctt atatttagtt ttgcacctta
    54121 ttacttcact acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga
    54181 aaaaactgtg agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc
    54241 agtggaataa tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat
    54301 aacccatttt gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca
    54361 ggcagttagc gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt
    54421 aatacccagt ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg
    54481 acactctgcg cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga
    54541 ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag
    54601 aataatgagt aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg
    54661 cgcgttgccc ttgtagatgg gcagcgtctt tatgacctgg atatcgaaag tccagggcac
    54721 gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa
    54781 gctgcttttg ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc
    54841 cgcgaatatt tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg
    54901 cgtgaaggtc aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg
    54961 gcattaacca cctttatcag tctggcgggt agctatctgg ttctgatgcc aaataacccg
    55021 cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca
    55081 ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc
    55141 aaatctgctg aggcgctgca atgggattta agcttccgcc tgaaacactg ggaagccatc
    55201 aaaaaagccg ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc
    55261 gttcgcgcat tccgcgatta cttacgtcag gacatcggcg aaatccttat cgataacccg
    55321 aaagtgctcg aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc
    55381 aaaatcaaac tgtataccgg cgagatcccg ctgttcagcc actaccagat tgagtcgcag
    55441 atcgagtccg ccttccagcg tgaagtccgt ctgccgtccg gtggttcgat tgttatcgac
    55501 agcaccgaag cgttaacggc catcgacatc aactccgcac gcgcgacccg cggcggcgat
    55561 atcgaagaaa ccgcgtttaa caccaacctc gaagctgccg atgagattgc tcgtcagctg
    55621 cgcctgcgtg acctcggcgg cctgattgtt atcgacttca tcgacatgac gcctgtacgc
    55681 caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt
    55741 caaatcagcc atatttctcg ctttggcctg ctggaaatgt cccgccagcg cctgagccca
    55801 tcactgggtg aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt
    55861 gacaacgaat cgctgtcgct ctctattctg cgtttgatcg aagaagaagc gctgaaagag
    55921 aacacccagg aagttcacgc cattgttcct gtgccaatcg cttcttatct gctgaatgaa
    55981 aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg
    56041 ccaaacgatc agatggaaac cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa
    56101 acgccgacct taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct
    56161 gaagaagagt tcgctgaacg taagcgtcct gaacaacctg cactggcaac ctttgccatg
    56221 ccggatgtgc cgccagcgcc aaccccagct gaacctgccg cgactgtcgt agccccagca
    56281 ccgaaagctg caacggcaac accagcagct cctgcacaac ctgggctgtt aagccgcttc
    56341 ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca
    56401 acaccgaaag cagaagcgaa accggaacgt caacaggatc gtcgcaaacc tcgtcagagc
    56461 aaccgccgtg accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat
    56521 aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc agcagactgc cgagacgcgt
    56581 gagagccgtc agcaggctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg
    56641 ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg
    56701 aaggcgctga atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt
    56761 ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc
    56821 gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt
    56881 gttcaggaag ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg
    56941 caaactgcac cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg
    57001 ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc
    57061 tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct
    57121 ccggaactgg cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta
    57181 caagttgaag agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactggggtc
    57241 cctgtcgccg ccgctgtcga acctgttgtt agcgcaccgg ttgttgaaga agtggctgaa
    57301 gttgtagaag cccccgttca gattgccgaa ccgcaaccgg aagtggttga aacgacgcat
    57361 cctgaagtga ttgccgccgc agtaactgaa cagccgcagg tgattaccga gtctgatgtt
    57421 gccgtagccc aggaagttgc agaacacgca gaaccggtgg ttgaaccgca ggaagagacg
    57481 gcagatattg aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt
    57541 gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac ggtagctgcg
    57601 gttgaacctg aggtcaccgt tgagcataac cacgctacgg tgccgatgac gcgcgctccg
    57661 gcaccggaat atgttccgga tgcaccgcgt cacagtgact ggcagcgccc tacttttgcc
    57721 ttcgaaggta aaggtgccgc aggtggtcat acggcaacac atcatgcctc tgccgctcct
    57781 gcgcgtccgc aacctgttga gtaataatta gctcaagtaa tcaagccctg gtaactgcca
    57841 gggctttttt atttcatctt tgaatcaaca tattaaaatt catcgaatgg catccttgct
    57901 aaccaacaat gcaaaatagg caaaaaaatg cccgccccgg cgatcccaga gcaggcagac
    57961 aaaacatacc cagtttcatg atatgtttta aagcgaaatt atttgttgag ctggaagagc
    58021 gacaatccct gcatatcggt aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg
    58081 atgtaagatg aaatagttgc attccagtcc acatcaacca gatcgctcat ctgctgcgtt
    58141 tgccctaaag cgcgatcgct acctaatgaa tccagcgact ccagttcgtt cagctgcgtg
    58201 cctaattccg cgcgaacggt cagcacattg ttcagcgagt ttttcagtcc gcggttggtt
    58261 ttatctaacg ccgcagcggc ggtttcttta tccgcttcgc tatccgcgac cggcgttttc
    58321 agggctgcga tggcgctatc cagcatggca aaaagattgg tttcagaagc gctaccgtct
    58381 ggttccgcta ccgcgttact ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc
    58441 accatcgaac gcgaagcatc gacctgttgc ttaatacttt ctgttccgcc gacataatca
    58501 ccgttagcct cactaaacgg cgcagtctct gttttataac cggcaaaaat gtagcgccca
    58561 ttaccgtcag tggtgttcgc cagattcagc aactggtcac gaagcccctg aatatccgta
    58621 gccagcgagg cccggtcatc gtcactcaag gtgccattgc tggcgtagac aattttttcc
    58681 tgagcattct ggatagcagt ggtgacctgg ctaagtacac tctcttccag tgacactttt
    58741 tgagtggcga aagtacgcgc cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga
    58801 acaaccgctt gtgatgcagc aatgggatca tctgaagggt taacgactcg cttacccgtc
    58861 gacatctgtt cgccgtactt catccattct gcctgagaat tggtgatacc acgcatgttt
    58921 tgctggtaca tcatctgggt actgaaacgc attttatctc cccttagcga atgttaatca
    58981 gcgcatcaaa aatcgcgttt gccgtctgca gaacctgcgc attcgccagg taatactgct
    59041 gaaaacgttg cagatttccg tactcctcat cgagattgac accggaaatc gactgctgct
    59101 gattggaaag ctgcgtcacc acattacctt gcgtggtgct actggttttc aacgtcgcgg
    59161 ttttattacc gatatcactc actaacgagg cataagcgtc gttaaaggat ttcgcaccgc
    59221 ccaccgtttt actattgctt tgcagatcca gcagggcctg accgttgcgg ttatcgctat
    59281 cacccgcatc tttttcgctc gccatcgcta ttttcgcttc gtcggtgatt aatacatcca
    59341 tgttgacgat ggcgtcactt actggtttca gcgtgaagct gtcgttaacg gcaggcgttc
    59401 ctgtaaacgt caactccaga ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg
    59461 taaaagtggt attgctggca aggcgggtga cctgccactg attattatcg aacgagattt
    59521 tgtaatctgt cgccagtacc acggaggcat cagttaccgt ggcaccgatc gcaacgtcac
    59581 ctttgttttt cgtgttttgc agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag
    59641 catcgccgtt agcatcaaat ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca
    59701 gcgccagttg tccaagcgta ttacgcgtct ggtccagctc ctgagaacgg aatgtcagaa
    59761 tgccgcccag cgacccggta ttcagtaatt tctccgggat ctcaatattg cctgccgtcc
    59821 catcaacata agcgacagtc gtacgagaag ggtcagcgct ggaaggaact gccgccagtt
    59881 gccgcgccgt acttccctga accagtgagt aaccattggc caccgtgatg ttataagtgc
    59941 cgccatcctg aacgctgact tctacaccaa caatctggtt taattcgctc accagttgat
    60001 cgcgttgatc cagcagattg ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga
    60061 tttgatcgtt caggctggca atttgtttag cgtaattgtt gatctgatca acgctggcac
    60121 ctatcgcgat attgacctgt ttgtcctggt cgcgcagata ttgatcggtg gttttaaact
    60181 gattcaccaa cccttctgat ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt
    60241 tactcaccag cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg
    60301 tactggtgga gagcatattg tcgattttcg acatctgctc atagcgggcg gtcaggccgc
    60361 tactttgcgt ctgcgcagca cgtaactggt tggtaataaa cgcatcatac tcacgctgca
    60421 caccagaaac gtagacgcca ttgccaaccc agccgccagc gcccaacgtg ctattggcct
    60481 gcgccataat agtggtttgg cgggtatatc cggcaacgtt atagctggag atattattac
    60541 ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct catggcgtta ttgatcaagc
    60601 tggacatgga ggttccttat aagccttcaa ttacagagta ttatcggcag cgacccgccg
    60661 gacttgagtt attcagaaca gattatcaat gttcatgctg taggttttgc tcaccttgtc
    60721 gcttatcgat ttcatctgct ggatcatgtt ggtgagttta cgggcatagt gaggatcggt
    60781 agcatacccc gcgtcctgta gggcctgcgc cccctgctcc gcactcgcgg ctgtcgtcac
    60841 ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa tccgacaatg cttccagata
    60901 cgagctgtag acccgaaatt tcgcttttac tttcttcgct tcgccatttt catattcagt
    60961 cgtggtgatt tcagtaactg gacctttcca gttgccagag gctttgacgc caaacaggtt
    61021 atagctcggc tcgccgtttt cgcggcggat ttgccgttgc ccccaaccag actccagcgc
    61081 cgcctgagcg aggatcaaat gatgtggcac accgctttgc tggcttgcca gttgggcggg
    61141 cagcgagagt tgcgcgagga atgctttact gtcacccggc agcgaatcat cgtagttacg
    61201 tggcacggcc ttttgcacca gctgcgaaag cgcctgattt tgataacgca ccacagtttc
    61261 gagcgggaat ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt gttctggcgt
    61321 catctgttta accatcatct ctgcaagccc cagacctttg cccgccgtca tctgttgggc
    61381 aatctgctgg tcatacatac tggtatacag gcgagtgtgc tcgctgctga acaggccatc
    61441 ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc cttccacctg
    61501 acgggccacc ggacggatat ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt
    61561 gagtgattgc gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg
    61621 atttccagtt ttgcccgcag acatcccgca ctttgcattg attgcagtat ggacatcaga
    61681 tccatcggcg tagcgcccag cgcattgagc gcgcgcacca cgttattgag gctggcgctg
    61741 gaacgtacgc tttgcagcga accgccgctc tggcgtaaat cgatctgcgt ttgtggagta
    61801 accacagtct gtccaccacc aaacggtgta tctggctggc tgacattggc ctgacgattg
    61861 actgttactg agagatttcc ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc
    61921 atcaccaccg aaccggtgcg cgagttaatc actactttag cgtcctgcgg ggtgacatta
    61981 acctgcatat tctggatatc tgcaaggaaa cggacctggg aactgttgcc acttggtacg
    62041 cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc catatccacg cacgcggttg
    62101 atggtgtcag cgatttgctg cgccatgctg aaatcttcgt cgttaagttg caaattaagg
    62161 gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc attggtgatc
    62221 cgtccaccgt tcagttggtt cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg
    62281 ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtcaacgcc cttaagcggc
    62341 gtcatcaaca acgtacctcc acgcaagctt ttggcatttc ccatggaaga aaccaccaca
    62401 tcgatggttt gcccctgacg tccaaacgga ggaagtgact ctgtcaccat taccgcagcg
    62461 acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg
    62521 ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct ggtcaccggt gccatccagc
    62581 cccaccacca gaccatagcc aatcagtgag ttttgcctta ccccctgaac actggtgaga
    62641 tcgcgaatac gctcagcctg agccgccgtc gtaaccagta gaagaattaa tgcagagaga
    62701 aatttaatca ccacagcctc acttacattg gcgacaggtt aaggaagaaa cgctgcaacc
    62761 agcccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaatg cgcgcatccg
    62821 ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta accacgccag
    62881 agaagcgaat aaattcggta ccctgattaa tggcaatctg tttttcaccc accacatgca
    62941 ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc
    63001 tggcattggc cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg acatcggcac
    63061 gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca aaattagttt
    63121 taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc tcctgcaaca
    63181 cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc
    63241 catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg
    63301 gaaccggttg tgcactggtc gccccctgca ccagcggcgt ggagggtatc caggcgcagc
    63361 cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg tttttttgca
    63421 tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc caccggttaa gccttagagt
    63481 tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa
    63541 gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt agacgtttca
    63601 acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc gttcggtaca
    63661 ccagaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagccc ggtgtcattc
    63721 ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt
    63781 acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg aatggtgatc
    63841 gctggctgca cctgaaaacc accagctgcc cgttctgatc cacctggaaa gagccgtcac
    63901 gggtataggc tgatgaacca tctggcaaca tcacctggaa aaagccctgc cctttaatcg
    63961 cgacatcttt gctgttgttg gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg
    64021 cgaccgggcg tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag
    64081 actgtgcccc cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac
    64141 gcttaaaacc gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt
    64201 gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact gatcataaaa tgtcctgtag
    64261 tggattagtg acaaagtgca tatgtcttct tgccggatgc ggcgtaaacg ccttatccgg
    64321 cctacaagtg ctcgcaaaat cgataaattg cgattgcccc catagacccg ataaacttgc
    64381 gcatcgggca acttatactt gtcatgtatc ctttcaatta actcatcgac agcagttggt
    64441 tggcacggcc tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc
    64501 gcgcgctggc aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa
    64561 cccccgacat cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg
    64621 tttctgcgct taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt
    64681 tcaccagttt cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg
    64741 ccgagattgt gccatcggca gcaatagtga tttccgctcc ttccggcaca gcaattggcc
    64801 ccgcctcgcc tatcacctga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa
    64861 tgctgccatt acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccagccat
    64921 cctgctgcaa cgcgacgtcc agcgggcgcg aggtgtaatc cattttgccg ggcgtcatat
    64981 ctgcgcccgg cgttgacgcc gtgaccaacg tgcgcgtggg cagagaaagc ccttccactg
    65041 gcaccgcgcg taaagcattc aactgcgcgc gaaaaccggg cgttgaggca ttggccagat
    65101 tactggcggt taccgcctgt tgattcagtg tctggctggc tgctcccatc gcggtataaa
    65161 ttgcgtgatc cattgagcta tcccgtcagc gattagcgta agttaaccag cgtgttgagg
    65221 atctggtcct gagttttgat ggtctgggcg ttagactgat agttacgctg ggcaacgatc
    65281 atattgacca gttctttact gagatcgaca ttggacgctt ccaacgcacc gttggtcagg
    65341 gtgccaaagt tgcccgtccc ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca
    65401 gaccagacgt tgtcgccttc ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca
    65461 atctgcccca gcagttgtgt ttgttcgttg gaatagttgc cgacaaccgt accgtcatca
    65521 ttgatttgat aactcaccag atcgcccggt ttgtagccat tctgggtggt tgccacaata
    65581 ttgttagcgc cggtattttg ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga
    65641 tctgcgccgt taattgcgcc agtggtaata ttctctgttg gatttgaggt cagaacgcca
    65701 ttggcattaa acaccagctt cattgcaggc tcggctgtac ctgttggatc actgctatcc
    65761 tgggtgtaga cctgccagtt attatccccg gtcttcacaa agtagacgct catgtcatga
    65821 gcattaccct gactgtcgaa aacagtcacc gaaccttttt tgttatagct atccgcattg
    65881 ctggcgctga atggcgtaac agtaggaagc ggatcactgg aattcaggtt gatctgcatc
    65941 gacgccgtgg tggtagtttt cgctgccatc agggtattcg ggatcgaaat attggtcgga
    66001 ttcgcccctt gctgaatagt cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa
    66061 ccttgcatat tcaccaggtt acggttttca tccagcttaa attgtccgtt acggctgtag
    66121 aacaccgaac cgttgctgtc taccagacgg aaaaaaccgt tctggctgat agcaacgtcc
    66181 agacctcgcc cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat accggcaact
    66241 tttaccccca gtcccacttt cgaaccggca aacatatcgg caaaagaggc cgtgcctgat
    66301 ttaaagccgt aggtggcgga gttggcgata ttgttgccaa taacatcgag gttggtggcg
    66361 gcagcgttta atccgctaac cgcttgagaa aaggccatga ctgactcctg aaatgtgaag
    66421 gcttaaatta tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc
    66481 gtattaccgc tgttgccgcg gagcacaccc tgcaccagag caaactgcag cggctgggca
    66541 accagttgtg taccaccgtt actggcgcta atcgctacat tgtaagaacc gttcggcgca
    66601 gtgctgccat cagtcaacgt accgtcccag gtgaaactgt ggactccggc ggtcagttca
    66661 ccaatatcaa tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc cgtaactttg
    66721 tctgccgcct gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc cccttcttca
    66781 ctgccggttc ccgcaagaac agtggtgccg gggatcatca cgccgtgacc gatcaggtta
    66841 ctggcctgta acgactggct gttatcaatc tgtccggaaa tagatccgag cgtggtattg
    66901 agtttttcaa tcccgctgac cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt
    66961 tccattggat tggtcgggtc ctggtttttc agctgcgcca ccaacaaagt cagaaaactg
    67021 ctttgtaagt ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt actgacgccg
    67081 gtatttgtcg gatcggtggt ggttaccgca atggacatag ctttctcctt tattgaccga
    67141 gcgtaagggt tttcagcatc atgcttttca ccgtgttgag cacttcaaca ttggcctgat
    67201 agctgcgtga cgccgacatg gtgttaacca tctccccgac gacatcaacg ttcggcattt
    67261 ttacgtagcc ctttgcatcc gccagcggat tacccggttc ataaaccagt ttgtccgggg
    67321 cctgactttc tataacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt
    67381 taacctggaa taccacctgt tttgcccgat atggctgtcc atcgggaccg gtcacgctat
    67441 cagcattcgc cagattactg gccgccacgt tcaagcgctg ggactgggca gttaacgccg
    67501 acccggcgat atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt
    67561 tcatcatgcc tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt
    67621 tatcggcaaa ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca agcgaaggct
    67681 ggtccggaat acggtattgc agttctgcgg taggaggcgt cagcgcctgc gccggaatgt
    67741 gttgcgttga ggtcatcgtc agtgcaacca cactggttgc atcccgtcca cgttgcatga
    67801 cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat
    67861 tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt
    67921 gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct
    67981 gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg
    68041 ctatttatgc cgttgatgac cattgcggac aggtacaatt cacgttgtag aaatggctgg
    68101 gggcgaaaat gctggcaata aaacgtagcg tggcgatcat ctcgatacta ttcactccgt
    68161 taagtgcggc gagtaatctc acatcgcaat tgcaaacctt ttttagcgcc cagctcgcgg
    68221 gggtaagtga tgaggttcgt gtttctattc gtacagcgcc caatctacta ccgccgtgcg
    68281 agcagccatt gctttcgatg agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg
    68341 cacgctgcgg taacgacaaa cgatatttac aggttaatgt acaggccaca ggaaattatg
    68401 tggttgccgc gatgcccatt gtgcggggag gaaagctgga agctggcaat gtcaaactaa
    68461 aacgcggacg gcaggatacc ctgccaccgc gtacggtgct ggatatcaat caacttgttg
    68521 atgccgttag cctgcgcgat ctatcacccg atcaacctat ccagttaacc cagtttcgcc
    68581 aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat cgccagcggt gacgggttta
    68641 gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt cacacagaat gcgcgggtgc
    68701 gcatggtatc gggactggtg gtcagcggcg ttgttgatgc agatgggaat attcttataa
    68761 accagtaagc tgttaaagat tacccgtccc ttgccgataa ataagcaaca catgataaaa
    68821 gcgccctcaa tgaggaataa accatgagta ttgatcgcac ttcgcctctg aagcccgtaa
    68881 gcaccgttca accacgcgaa accactgacg cgtcggtaac gaacacccgg gcggcaaaaa
    68941 caacagcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac
    69001 ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg
    69061 gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct gctcaacgaa gcgcagcaag
    69121 acttgcagag taactgatcg tatgacacgt cttgcagaga tcctcgacca gatgtccgct
    69181 gtgcttaacg atctcaaaac ggtaatggat caagaacagc aacatctctc tatggggcag
    69241 atcaacggca gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg
    69301 gattacctcg aacagttacg caggaaagaa cccaatacag caaatagcgt tgatattagt
    69361 caacgctggc aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac
    69421 ggctggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg
    69481 catcaggaac cgacgctata tggggcgaac ggtcagacct caacaaccca tcgcggcggt
    69541 aaaaagattt cgatctgaat attatctccg gcctgcactg caggccggaa tgcattgtta
    69601 caccgtccgg cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta
    69661 cgcggcaatc cccgccagca cgaccgccat taaacgcagt aagcgccagg gcatggtacc
    69721 caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac
    69781 cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg
    69841 cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag
    69901 tgacagccca gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat
    69961 cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg
    70021 agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg
    70081 ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa
    70141 cggacccgaa agaatgccca acgcaaccgc actcggcagc gccaacagga aacaaagacg
    70201 caacccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga
    70261 cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc acaccggagg gaaactccat
    70321 taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat
    70381 ggtgttgatg attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg
    70441 tttcaccacg cgcattgctc cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc
    70501 gatcttcttc aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc
    70561 ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat
    70621 catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt
    70681 cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt
    70741 tagtagctgg ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac
    70801 catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt
    70861 cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt
    70921 atattccgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg
    70981 taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa
    71041 gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga
    71101 gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccaga cccacgggca
    71161 aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct acctggatta tggtgaattg
    71221 ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga
    71281 tacgttgcgc cagcactgcc tgttcgccgg cggtttgcag aactgtctga ttttgcacac
    71341 attcaatgaa gtgccgcgca cagccgacaa atccacgttg ctcaagagtg ctctgccaac
    71401 caggaatcgg tttatgcact acgccctgcc cgcgctcctc acgccattcg cgcatatccg
    71461 taatgtcgat gagcgcatcg tcagtcacgg cctgcacggt ttcacgctga cttccggcac
    71521 ggcgatgcat acaggtggtg atctgcaaag gatcggccga aaaatggtgc tcggcaaaca
    71581 gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg
    71641 acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat
    71701 cgtgtggccc gacactattg ctacgatgtt tatccattct tagcgaagct gcggtagcga
    71761 gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg
    71821 tcagtttttt ccgcgccgcc agttccacca gccgttcagc atcgcgcaga ttttctgcca
    71881 gcggtttatc gacacagaca tgtacacccg cattgagtaa cgtactgacc acgtcaaagt
    71941 ggctggcggt gctggaatgc acaaaaaccg catcgcaact ggcggcaagg ctggataacg
    72001 aatcggcata aggaatgcgc cagctttcac aaattggcag ggctttcgcg cgcgtaggcg
    72061 accaggctcc ttgtaacatc cagtcagacg ctgccgccag caccggtaac cacgcttttt
    72121 gcgcaatgcc gcctaatccc acaacgccga tacgtaattt tttcacttta atctcccaga
    72181 tgggccagca acgaatcaag acgctgtttc agttctgcca cttcgatttc cagggcttcg
    72241 acgcgggcct gtaaatcacc atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc
    72301 tctacctcac cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc
    72361 agacgcacca caaaaggacc atcttcgcga tttgccagtt gttccagcgt cgactccact
    72421 tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc
    72481 tgggcaccac gcaataacaa cgtggtgatt aacgccactt ccgctgcgct cagtttcaga
    72541 tcgccaaatt ctgaattaca aaaacgttgt tcatatttgg tgacccgatt accaaagcca
    72601 ctcaccgtgc gcagataatg gcgtttgacc agattatcca gttgctcctg cacttcagat
    72661 tcactcagat tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca
    72721 ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc
    72781 acgcgagctt caagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt
    72841 ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag
    72901 atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg
    72961 tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata
    73021 acgaatggct gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat
    73081 cgaataaccg agatagcagg catgaaaaga gccacgaaca acattggaaa aattggcaac
    73141 gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt
    73201 atgaaattcg ttaatcatcc ccagcctggc ctgccagcct gatggataac agtggctttc
    73261 gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgcaag
    73321 acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac
    73381 ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt
    73441 aatacgctgt aagtattact atacctgcgc aaccagcccc tgtgaaaagt ccaaaatgcc
    73501 attttctgca ttattcacat atttgataaa aactctctat caaagcgatg ttttgataaa
    73561 aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct
    73621 catcttttat ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa
    73681 atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct
    73741 gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct
    73801 cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg
    73861 ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac
    73921 aatgggtatc gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg
    73981 cggcacgttg tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca
    74041 acgttgtcgt tttttctcgc tgttgatgat gcaggacagt gccggtgcgg tcattggcgc
    74101 attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt
    74161 tttgtttgtg ctgtgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac
    74221 cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac
    74281 ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc
    74341 aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga
    74401 agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg
    74461 tctggaacac cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg
    74521 catggtcagc ggtctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat
    74581 cattgccgag cctgcgcgtg aaaccttaag tgctttgctg gcggacgcaa gagctcgcgg
    74641 cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg
    74701 tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat
    74761 gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg
    74821 cgcaacgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca
    74881 tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca
    74941 ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt
    75001 acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt
    75061 tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta
    75121 tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga
    75181 gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc
    75241 ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc
    75301 cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag
    75361 cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact
    75421 ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat
    75481 caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa
    75541 tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt
    75601 gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt
    75661 gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg
    75721 ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca
    75781 ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt
    75841 tacccggcgt ggctgacgcc catattgttc tgacaaactt gatcagccaa attggtcgcg
    75901 aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg
    75961 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    76021 aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa
    76081 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    76141 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    76201 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    76261 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    76321 ttgtgtccgg caaaaacatt ccttcagccg gagcatagag attaatgact gcaccatccc
    76381 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    76441 taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc
    76501 tggctccgcc cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg
    76561 ccgtacctgc cgggcacgat ttcaccccat tgatgacctg ttatttaaca gattcgctgg
    76621 atcctaatga gctggagcgc ggatttaatg aaggcgtgtt caccgctgca aaactttacc
    76681 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    76741 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    76801 cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc
    76861 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    76921 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    76981 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    77041 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    77101 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    77161 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    77221 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatgggt
    77281 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    77341 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    77401 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt atttaacctg tataaataac
    77461 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    77521 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    77581 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    77641 attggcgcaa caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    77701 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg
    77761 ccccggcttt tttattttac ttacgaattt tgagtacaaa atttctacac ttcctcaaaa
    77821 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
    77881 ttaaaa
//
LOCUS       DAJTYZ010000003        69883 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000003 DAJTYZ010000000
VERSION     DAJTYZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69883
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.003"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..674)
                     /locus_tag="OO596_000168"
     CDS             complement(<1..674)
                     /locus_tag="OO596_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCR6716143.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="OO596_000169"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="OO596_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6716144.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="OO596_000170"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="OO596_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6716145.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="OO596_000171"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="OO596_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6716146.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="OO596_000172"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="OO596_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6716147.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="OO596_000173"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="OO596_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR6716148.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="OO596_000174"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="OO596_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6716149.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="OO596_000175"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="OO596_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6716150.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9752)
                     /gene="hyfD"
                     /locus_tag="OO596_000176"
     CDS             complement(8400..9752)
                     /gene="hyfD"
                     /locus_tag="OO596_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6716151.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9728..9838)
                     /locus_tag="OO596_000177"
     CDS             complement(9728..9838)
                     /locus_tag="OO596_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR6716152.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="OO596_000178"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="OO596_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6716153.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="OO596_000179"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="OO596_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCR6716154.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="OO596_000180"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="OO596_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6716155.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="OO596_000181"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="OO596_000181"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6716156.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="OO596_000182"
     CDS             complement(14171..14743)
                     /locus_tag="OO596_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6716157.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="OO596_000183"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="OO596_000183"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6716158.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="OO596_000184"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="OO596_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6716159.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="OO596_000185"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="OO596_000185"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6716160.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="OO596_000186"
     CDS             17912..18760
                     /locus_tag="OO596_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6716161.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="OO596_000187"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="OO596_000187"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6716162.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRSRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="OO596_000188"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="OO596_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6716163.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="OO596_000189"
     CDS             complement(21672..21872)
                     /locus_tag="OO596_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6716164.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="OO596_000190"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="OO596_000190"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6716165.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="OO596_000191"
     CDS             complement(23031..23387)
                     /locus_tag="OO596_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6716166.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23769..23828
                     /gene="ypfM"
                     /locus_tag="OO596_000192"
     CDS             23769..23828
                     /gene="ypfM"
                     /locus_tag="OO596_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6716167.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23927..27040)
                     /gene="acrD"
                     /locus_tag="OO596_000193"
     CDS             complement(23927..27040)
                     /gene="acrD"
                     /locus_tag="OO596_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6716168.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27204..28904)
                     /gene="narQ"
                     /locus_tag="OO596_000194"
     CDS             complement(27204..28904)
                     /gene="narQ"
                     /locus_tag="OO596_000194"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR6716169.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29110..31089
                     /gene="aegA"
                     /locus_tag="OO596_000195"
     CDS             29110..31089
                     /gene="aegA"
                     /locus_tag="OO596_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6716170.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31157..31732
                     /gene="nudK"
                     /locus_tag="OO596_000196"
     CDS             31157..31732
                     /gene="nudK"
                     /locus_tag="OO596_000196"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6716171.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31858..32901
                     /locus_tag="OO596_000197"
     CDS             31858..32901
                     /locus_tag="OO596_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6716172.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32997..35000)
                     /gene="tkt"
                     /locus_tag="OO596_000198"
     CDS             complement(32997..35000)
                     /gene="tkt"
                     /locus_tag="OO596_000198"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6716173.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35020..35970)
                     /gene="tal"
                     /locus_tag="OO596_000199"
     CDS             complement(35020..35970)
                     /gene="tal"
                     /locus_tag="OO596_000199"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6716174.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36259..38538
                     /gene="maeB"
                     /locus_tag="OO596_000200"
     CDS             36259..38538
                     /gene="maeB"
                     /locus_tag="OO596_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6716175.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38831..39166
                     /gene="eutS"
                     /locus_tag="OO596_000201"
     CDS             38831..39166
                     /gene="eutS"
                     /locus_tag="OO596_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6716176.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39179..39658
                     /gene="eutP"
                     /locus_tag="OO596_000202"
     CDS             39179..39658
                     /gene="eutP"
                     /locus_tag="OO596_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6716177.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39633..40334
                     /gene="eutQ"
                     /locus_tag="OO596_000203"
     CDS             39633..40334
                     /gene="eutQ"
                     /locus_tag="OO596_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCR6716178.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40331..41134
                     /gene="eutT"
                     /locus_tag="OO596_000204"
     CDS             40331..41134
                     /gene="eutT"
                     /locus_tag="OO596_000204"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCR6716179.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41131..42147
                     /gene="pta"
                     /locus_tag="OO596_000205"
     CDS             41131..42147
                     /gene="pta"
                     /locus_tag="OO596_000205"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6716180.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42186..42479
                     /gene="eutM"
                     /locus_tag="OO596_000206"
     CDS             42186..42479
                     /gene="eutM"
                     /locus_tag="OO596_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCR6716181.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42586..42873
                     /gene="eutN"
                     /locus_tag="OO596_000207"
     CDS             42586..42873
                     /gene="eutN"
                     /locus_tag="OO596_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCR6716182.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42885..44288
                     /locus_tag="OO596_000208"
     CDS             42885..44288
                     /locus_tag="OO596_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCR6716183.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44299..45135
                     /gene="eutJ"
                     /locus_tag="OO596_000209"
     CDS             44299..45135
                     /gene="eutJ"
                     /locus_tag="OO596_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCR6716184.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMVDIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45125..46312
                     /gene="eutG"
                     /locus_tag="OO596_000210"
     CDS             45125..46312
                     /gene="eutG"
                     /locus_tag="OO596_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCR6716185.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46629..47855
                     /gene="eutH"
                     /locus_tag="OO596_000211"
     CDS             46629..47855
                     /gene="eutH"
                     /locus_tag="OO596_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCR6716186.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47852..49255
                     /gene="eutA"
                     /locus_tag="OO596_000212"
     CDS             47852..49255
                     /gene="eutA"
                     /locus_tag="OO596_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6716187.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49267..50628
                     /gene="eutB"
                     /locus_tag="OO596_000213"
                     /pseudo
     CDS             49267..50628
                     /gene="eutB"
                     /locus_tag="OO596_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            50649..51536
                     /gene="eutC"
                     /locus_tag="OO596_000214"
     CDS             50649..51536
                     /gene="eutC"
                     /locus_tag="OO596_000214"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR6716188.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51546..52205
                     /gene="eutL"
                     /locus_tag="OO596_000215"
     CDS             51546..52205
                     /gene="eutL"
                     /locus_tag="OO596_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6716189.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52218..52286
                     /locus_tag="OO596_000216"
                     /pseudo
     CDS             52218..52286
                     /locus_tag="OO596_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="ethanolamine utilization protein; involved in the
                     degredation of ethanolamine; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutK"
     gene            52345..53397
                     /gene="eutR"
                     /locus_tag="OO596_000217"
     CDS             52345..53397
                     /gene="eutR"
                     /locus_tag="OO596_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR6716190.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53403..54302)
                     /gene="hemF"
                     /locus_tag="OO596_000218"
     CDS             complement(53403..54302)
                     /gene="hemF"
                     /locus_tag="OO596_000218"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6716191.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54306..55175)
                     /gene="amiA"
                     /locus_tag="OO596_000219"
     CDS             complement(54306..55175)
                     /gene="amiA"
                     /locus_tag="OO596_000219"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6716192.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55389..55814
                     /locus_tag="OO596_000220"
     CDS             55389..55814
                     /locus_tag="OO596_000220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6716193.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            55801..56250
                     /locus_tag="OO596_000221"
     CDS             55801..56250
                     /locus_tag="OO596_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6716194.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56311..56886
                     /locus_tag="OO596_000222"
     CDS             56311..56886
                     /locus_tag="OO596_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6716195.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            56982..57881
                     /gene="yfeX"
                     /locus_tag="OO596_000223"
     CDS             56982..57881
                     /gene="yfeX"
                     /locus_tag="OO596_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6716196.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(57939..59243)
                     /gene="pbp4b"
                     /locus_tag="OO596_000224"
     CDS             complement(57939..59243)
                     /gene="pbp4b"
                     /locus_tag="OO596_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCR6716197.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59248..59421)
                     /locus_tag="OO596_000225"
     CDS             complement(59248..59421)
                     /locus_tag="OO596_000225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCR6716198.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(59434..60672)
                     /gene="murP"
                     /locus_tag="OO596_000226"
                     /pseudo
     CDS             complement(59434..60672)
                     /gene="murP"
                     /locus_tag="OO596_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(60676..61572)
                     /gene="murQ"
                     /locus_tag="OO596_000227"
     CDS             complement(60676..61572)
                     /gene="murQ"
                     /locus_tag="OO596_000227"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6716199.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            61736..62593
                     /gene="murR"
                     /locus_tag="OO596_000228"
     CDS             61736..62593
                     /gene="murR"
                     /locus_tag="OO596_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6716200.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            62722..63513
                     /gene="ucpA"
                     /locus_tag="OO596_000229"
     CDS             62722..63513
                     /gene="ucpA"
                     /locus_tag="OO596_000229"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6716201.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            63684..64700
                     /gene="cysP"
                     /locus_tag="OO596_000230"
     CDS             63684..64700
                     /gene="cysP"
                     /locus_tag="OO596_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6716202.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            64700..65533
                     /gene="cysT"
                     /locus_tag="OO596_000231"
     CDS             64700..65533
                     /gene="cysT"
                     /locus_tag="OO596_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6716203.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65533..66408
                     /gene="cysW"
                     /locus_tag="OO596_000232"
     CDS             65533..66408
                     /gene="cysW"
                     /locus_tag="OO596_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6716204.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66398..67495
                     /gene="cysA"
                     /locus_tag="OO596_000233"
     CDS             66398..67495
                     /gene="cysA"
                     /locus_tag="OO596_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6716205.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            67629..68540
                     /gene="cysM"
                     /locus_tag="OO596_000234"
     CDS             67629..68540
                     /gene="cysM"
                     /locus_tag="OO596_000234"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6716206.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68543..68911)
                     /locus_tag="OO596_000235"
     CDS             complement(68543..68911)
                     /locus_tag="OO596_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6716207.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69016..69867
                     /gene="pdxK"
                     /locus_tag="OO596_000236"
                     /gene_synonym="thiJ"
     CDS             69016..69867
                     /gene="pdxK"
                     /locus_tag="OO596_000236"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6716208.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16614 a        18234 c        19303 g        15732 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctggccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagttatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacatt actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccca ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgctattctg attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgctcg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcgggtcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa tggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt
    23401 tcgatttatg agaataaata ctcatttaag ggtgaaaaca ttttgcctgc aaatgattat
    23461 caggaaatat aacattatcg aagtattcaa attaacaact attaaataat attaaacatt
    23521 tttaacgtaa acattaagaa tattcatctt gttatctgtc tgtattttaa cctgaattaa
    23581 atcagtatta gatgccgtat gaatatctcg aaatgttgct gtgtatcaca tacaaatcat
    23641 caccgcagga ccatagtatt tacataagat ttgtcccccc ggaggtagtc atcgcagcaa
    23701 tcgagtaaat tagcttcacc acggtatatt gtttccgtag aatacccata attataagag
    23761 aggttgttat gattgaacgt gaactgggga actggaaaga ctttatcgaa gttatgcttc
    23821 gtaagtaatt cctgagacaa agtataaaca gcaagaaccc gctgagcagg ttcttaatcg
    23881 gaaagttgaa aaaggcgaca cattggcatg tcgccttttt tattgcttat tccgggcgcg
    23941 gctttagcgg gaagcggcgg cgcaccagca caaagaacag cggcacgaag taaatagcca
    24001 gaatagtggc tgaaatcatc ccgcccatta cgccagtacc caccgcatgc tgaccaccgg
    24061 aaccggcacc cgtgctggtt gccattggca atacgccgaa gataaatgcc agcgaggtca
    24121 tcagaatcgg gcgtaaacgc tgacggcagg cgtggagcgt cgcttcaaac aggtcgtggc
    24181 ctttttggtt catctcgtta gcaaactcga cgatcaggat ggcgtttttc gccgataaac
    24241 caatgaccgt taacaggccc acctggaagt aaacgtcgtt ttccagcccg cgcatccagg
    24301 ttgccagcag cgcgccgatt acccccagcg gcacgaccag cattaccgag aacggcaccg
    24361 accagctttc atacaacgca gccagacaca ggaataccac cagcaaggaa atggcgtaca
    24421 gcgccggagc ctgcgcgccg gaaagtcgct cctgatacga catcgccgtc cactccagac
    24481 caaagccgtt tggcagctgc ttcactaacg attccataat atccatcgcc gtaccggtac
    24541 tgacccccgg tgcggcttcc ccaacaatct caaccgcaga ataaccgtta tagcgttcca
    24601 gacgcggcga gcctgtttcc cagcgtgagg tcgcgaaagc agagaagggc accatgccgc
    24661 catctttatt tcggacatac cagagattga tgtcatctgg cagcatgcga tacggcgcag
    24721 ctgcctgcac atagactttc ttcacgcgac cgcgatccat aaagtcattc acatagctcg
    24781 aaccccaggc ggtttgcagt gtgtcgttaa tatcgtcgat agcaacgccc agcgcctgag
    24841 ctttacgctg gtcgatatca atctgcaact gcggactgtc gtcgaggccg ttatggcgca
    24901 cacgggttag ctccgggttt tccgccgcca gcgccagcaa ctggttacgt gctgccatca
    24961 gcgcatcgtg acctgctcca gcgtggtcct gcaactccat atcaaaaccc gcagaactac
    25021 caagaccgct aattgctggc gggctgctgg cgataacgcg agcttcttta atcttgttaa
    25081 acgcctttgt tgcacgctcg ataatggcaa acgaggtgcc ggtcttactg tcgcgttcgc
    25141 tccagtcttt caggcggata aacattcgcg ccacgttttg cccgttaccc ccagggccag
    25201 aaccaacggt ggcaaacacc gacatgatgt tgtctttttc atgggtgaag taatatttct
    25261 cgatttgctc aacgactttc agggtctgtt gttgggttga accgctgggc aattgtaccg
    25321 aggtagtaaa catgccacgg tcttccagcg gtaagaacga cgtcggcaaa cgcaggaaca
    25381 ggaacaccat gccgccaagc agcaggacat aaatcacaat ccagcgcagg ctgcggtgga
    25441 gaattttcgc cacccctttt tcgtagcgtt cggcgttgcg gttaaacatc tggttaaacc
    25501 aggcaaaaaa gcctttttgc ccgtgatgct cacctttttt cagtggctta agtagtgtgg
    25561 cacacagagc cggagtgagg atcatcgcta ccagtactga cagcaccatc gccgcaacaa
    25621 tggtaataga gaactggcga tagatggcac cggtggtgcc gccgaagaag gccattggta
    25681 caaataccgc cgaaagcacc atcgcaatcc cgaccagtgc cccctggatc tgccccatcg
    25741 atttacgtgt ggcttcgcga ggagtgagtc cttcctcact cataatacgt tcgacgtttt
    25801 ccaccaccac gattgcgtca tccaccagca gaccgatcgc cagtaccatc gcgaacatgg
    25861 ttaaggtgtt gacgctgtaa ccgaaggcgt aaagtacgga gaaggttccc atcaacacca
    25921 ccggcacggc gatagtgggt atcagcgtgg cgcggaagtt ttgcaggaac agatacataa
    25981 cgaggaaaac cagggcgata gcttccagca gcgttttcac cacgtcttca atggaggctt
    26041 taacaaacga ggtggtttca tacgccacct tgtattccag tccgtgcggg aagtactgcg
    26101 ccagctcgtc gagacgattg agcaccagct ccgctgtcgc catttcgttt gcgccggagg
    26161 ccagttttac ccccagcccg gaggctggct taccattgaa gcggctaaga taatcgtatt
    26221 tctccgcccc catttcgacg gtggcgacat cgcccagcct tacctctgag ccgtcttgat
    26281 tcacccgcaa ggtgatatcg cggaactgtt ctggtgtttg cagcagtgac tgggcgttaa
    26341 tggtggcgtt gagcgcctgc ttatcgacgg aaggtgtacc accaagttgc ccaaccgcaa
    26401 tctgcgcgtt ctgtgactca atggcatcag tgacatcttt agccgtcatc tggaaactgt
    26461 tgagtttcgc cgggtccagc cagatacgca tggaatattg cgaaccatag gcatcgatat
    26521 ccccgacgcc attcacgcgg cttaacgggt cctgaatatt actggcaaca taatcggcaa
    26581 tatcctgttt atccatcgaa ccatcggtag agacgaaagc aatggtcaga atattggtat
    26641 cgccggtttt acgcaccgtc acgccctgat tttgcaccgc ctgcggtaac tttcgcatgg
    26701 ctgattgcag ctggttttgt acttgctgca cggcttcgtc cggatcggtg cctgctttaa
    26761 aacttaaagt gacagatgcc tgaccgatgc cactgctctg agatgacata tacatgagat
    26821 tatcgaggcc ggtcatattt tgctcgataa cctgggtcac ggtgttttcc agcgtctggg
    26881 ccgatgcgcc gggatagtta gcggtcactc gcacattcgg tggcgcgaga tcggggtatt
    26941 gttcaacggg caatgaaaaa atcgccaggg tccctgtcag acacaacagg attgccagca
    27001 cccaggcaaa aatggggcga tcaataaaga aattcgccat taaaagagga cctcgtgttt
    27061 ctgcgtatcg ttgtaggctt gacttgcttc actgtagcgg caaggtacga gccaatcgtg
    27121 gagaaaaaaa ggagttaatg taaattatca tgccctttta tactgaacct taagtgcaag
    27181 tattctttgg tcagtaggag gcattacatc aactgacttt cctcaccctc cgcagagcga
    27241 aagctaatac tcactaacgt gccgccgccg gaaggttgtg aaaaagtcag cgtcccaccc
    27301 agccgttccg cgcgttcgcg catgatattc agaccataat gaccttcggg ttctttcggt
    27361 tcaccgatac caatcccgtt atcacggata taaaccgtgt gattgccgtc cggcgcggtg
    27421 acgcaactga cggcgatttc gctggcgttg gcgtgcttca tcgcattcag caccgcttcg
    27481 cgaataattt gcaacaaatg cacctgcatt tgcgcatcca gcgccagggt tggcagacgg
    27541 cagtcgaggg tcagtttggc gctggtttga ttttgtaacg tatccagcat ttccctcaac
    27601 gcggagggga gatccgcctg ctgcagcgtc aggcggaaag tagtcaacag ctcgcgtaac
    27661 tgccgataag catcattcaa cgcctgggaa aagtcggcca tgatactttg tgcggtggcg
    27721 ttatcttccg gtatcgaacg cttcagtaac gtcaactgga tacgtaagta agaaagtacc
    27781 tgagccagcg agtcgtgcaa ttcgcgggcg atggttgcac gttcttccat caacaataac
    27841 tgctgaaaat gcttttgcgc ctgattaaag tacaaaccgc gtcccagcat cgacgaaacg
    27901 ctgttaagca gcggttctga tgatgaaacg tgactatttt gccagtgcag ttcgccgtaa
    27961 accgtctctt gcattgtcac cggtaaaatc tgcatcggca attccgggtt tggttgccct
    28021 tcgctaatcc gccagttttc accgacattt aactccagat attcagccgc ttcattgtcg
    28081 cgaacaatct gcaaaatatg gcggaaacaa tgcacatcaa tctggcttgt gttcagcgcc
    28141 tgcgaacatt gatacaacac ctccagacga cgcttggcct cgtggagatc gcgggtcttt
    28201 tcttctactg acgcctccag cgaaaggtac aatttatgca gctcgctcga catctggtta
    28261 aaggtttttg caagcagacc aagctcattc ggcagactgg tatccagcgg cggcgagtcg
    28321 aactgcccgt gttcaatacg ttgactggcg gtaaccagct gattcagcgg ggcaaccacc
    28381 tgatggcgta tacggcgcaa agtaaaaaag accagcgtga aaataccgat accgccagcc
    28441 agggaaatcg ccaccaccag cagcattttg cgttcagcgt agtgctgtaa agccagcacg
    28501 aacaggtcta tctgattaac ataattatta atattggcct gataccacgg caaatcgccc
    28561 ttgctgagcc gattattcat ctccagccag ttggcattca gatgcgcata gcgagttttt
    28621 actgcttctg gcacatacca gacgttgaga ttggttaata ccgatgaatg cagtgcctgc
    28681 tgaaacaact ggcgatgtgc attgagttgt ggactgccac tttgcaagtc gtagcccagg
    28741 cggtaactct gcatacgcag cgatccggca atattgatag cctcagcgtc gcgcaaactg
    28801 ctcgccagag ttagcagagc gatacccgtg gaaagaatcg acagcagcac aatgtaaaaa
    28861 aaggcccggg ccagactggc cgagacgggt cgtttaacaa tcacacgcgt cttctccaca
    28921 aaaaatcatt aatgttccag ttcgctaaaa cgcaatttag tcagtgatgg gattatttga
    28981 tctgccacag tttatagcga aatagttact tattctgggc acatgaacat tgccgattcc
    29041 cctgtaagtc gggtaataac aacactcata taaagaataa ggtttttaca accaaaaaag
    29101 aaggtcgtta tgaatcgttt tattatggcc aacagtcagc aatgtctggg ttgtcatgct
    29161 tgtgaaatcg cctgtgtcat ggctcacaat gatgagcaac atgtcctgag ccaacaccat
    29221 tttcatcccc gaattacggt tatcaaacat cagcagcaac gtagtgcagt gacctgtcac
    29281 cattgtgaag atgcgccctg cgcccgtagc tgccctaatg gcgcaatcag ccacgttgat
    29341 gacagcattc aggtcaatca gcaaaagtgt attggctgta aatcctgcgt ggtggcctgt
    29401 ccgtttggta cgatgcaaat cgtcctgaca cccgtcgcgg caggaaaagt aaaagccacg
    29461 gcgcataaat gcgacctttg tgcagggcgc gaaaacggtc ctgcctgtgt cgagaattgc
    29521 ccggcggacg cgctgcaact ggtcactgac gtcgcactct ccggcatggc gaaatcccgc
    29581 cgcttgcgca ccgcacgtca ggagcatcaa ccgtggcatg ccagtaccgc tgcacaagaa
    29641 atgccggtaa tgagtaaagt cgaacaaatg caggcaacgc ccgcgcgtgg cgagccggat
    29701 aaactggcga ttgaagcgcg caaaaccggt tttgatgaaa tttatctgcc attccgtgcc
    29761 gatcaggcac aacgggaagc ctcgcgctgc cttaaatgcg gcgagcacag cgtttgtgaa
    29821 tggacctgcc cgctgcataa ccatattccg cagtggattg aactggtgaa agccggaaac
    29881 atcgacgccg ccgtcgagct ttctcacctg accaacaccc tgccggaaat taccggacgc
    29941 gtttgtccgc aggaccgttt gtgtgaaggt gcctgtacta ttcgcgatga gcacggcgcg
    30001 gtaactatcg gcaacattga acgctacatt tcagatcagg cgttggcgaa aggttggcgt
    30061 cctgacttaa gccatgtcac caaagtggac aagcgggtgg cgattatcgg tgcgggtccg
    30121 gcagggctgg cctgtgcgga tgttctgacc cgcaatggcg tggcggtgac ggtgtacgat
    30181 cgccatccag aaattggtgg cttgctcact ttcggcattc cttctttcaa actggataaa
    30241 tccctgctgg cacgccgtcg ggaaatcttc agcgcgatgg ggattcactt cgaactcaat
    30301 tgtgaagtgg gtaaagatgt ctctttggat tcgcttttgg aacaatacga cgcggtcttc
    30361 gttggcgtag gcacttaccg ttccatgaaa gcgggtttac ccaatgaaga tgcgccgggc
    30421 gtttatgacg cgctgccgtt cctcatcgcc aacactaaac aggtgatggg actcgaagag
    30481 ctaccggaag agccgtttat caataccgcc ggactcaacg tcgtggtact gggcggcggc
    30541 gacaccgcga tggactgtgt gcgtaccgcg ctgcgccacg gcgcgagtaa cgtcacctgc
    30601 gcttatcgtc gtgatgaagc taacatgcca ggctcgaaga aagaggtgaa gaacgcccgc
    30661 gaagaggggg ccaacttcga atttaacgtc cagccggtgg cgcttgagct gaatgagcaa
    30721 ggtcacgttt gcggaattcg tttcctgcgc acgcgtcttg gcgagccgga tgcccagggg
    30781 cgccggcgac ttgtaccgat tgagggcagt gaatttgtca tgccagccga tgcggtgatt
    30841 atggtgtttg gcttcaatcc acacgggatg ccgtggctgg agtcgcacgg tgtaacggta
    30901 gacaaatggg gccgcatcat cgcggatgtg gaaagccagt accgttacca gaccaccaat
    30961 ccgaaaatct tcgccggtgg cgacgccgtg cgtggtgcgg atctggtggt taccgcaatg
    31021 gcagaaggac gtcatgcggc acaggggatt attgactggc tgggggtaaa atcagtcaaa
    31081 tctcactgat agcctgcgca gacaaacccg acttcacagc gtaatataat tgttcatttc
    31141 gcgctgtgga gtcggtatga cgcaacaaat caccctcatt aaagacaaaa ttctctccga
    31201 taactatttc accctgcaca acattactta cgatctcacg cgcaaagatg gcgaagttat
    31261 ccgccataaa cgtgaagttt acgatcgcgg taatggtgcg acgatcctcc tgtacaacgc
    31321 gaagaaaaag accgtggttc tgattcgtca gttccgtgtc gctacctggg ttaatggcaa
    31381 tgaaagcggg cagctgattg aaacctgcgc cgggctgctg gataacgacg aaccggaagt
    31441 gtgcattcgt aaagaagcga ttgaagagac gggctatgaa gtgggcgaag tgcgcaaatt
    31501 atttgaactg tatatgtcgc ctggcggtgt gactgagcta atccactttt ttatcgccga
    31561 atacagtgac aatcagcgcg ctaacgccgg tggcggtgtt gaagatgaag atattgaagt
    31621 gcttgagctg ccgttcagcc aggcgctgga gatgatcaaa accggcgaga tacgtgacgg
    31681 taagacggtg ttattgctta actatttgca aacgtcacat ttaatggact gaaaaataac
    31741 aataatattt cgttgtttat tattggctca atccgacaaa tctgattgag ccgcgctacc
    31801 tgcgcaacga agatacgact tgtgctgttt gtttgaactt ctggggtcgt accgtccatg
    31861 cgctatcgca ttttccttct cttttttttc gctttgttgc cgacgtcttt ggtgtgggcg
    31921 gcactagcgc aacgggcgtt ttccgactgg caggtcacct gtaataacca aaacttctgc
    31981 gtggcgcgca atacgggcga tcataatgga ctggtgatga ccctgagccg cagcgccggg
    32041 gcgcataccg atgccgtttt acgtattgag cgcggcgggt tgaagtcgcc ggatgcgtct
    32101 gaaggagaga tagcgccacg gctgctgtta gatggtgagc cgttggcgtt aagtggtgac
    32161 aagtggcgga tttcaccatg gttattagta accgatgata cggcaaccat caccgcgttt
    32221 ttgcagatga ttcaggaagg gaaggcgatc accttacgcg acggcaatca gaccatttct
    32281 ctgagtggct taaaagcggc gttgttgttt attgatgctc agcaaaagcg cgttggcagt
    32341 gaaaccgcgt ggatcaagaa aggggacgaa ccgccgctca gcgtaccgcc cgcgcctgcg
    32401 ctgaaagagg tcgcggtggt taacccaacg ccgacgccac tctcactcga agaacgcaac
    32461 gatttgctgg attatggaaa ctggcggatg aatggtctgc gctgctcgct tgatccgtta
    32521 cgtcgtgagg tgaatgtcac tgcgctgact gatgataaag cgctgatgat gatcagctgt
    32581 gaggcagggg cctataacac cattgatttg gcatggattg tgtcgcgtaa aaagccgcta
    32641 gcctcgcgcc cggttcggtt gcgtttgccg ttcaacaacg ggcaggagac gaatgaactg
    32701 gaactgatga acgcaacatt tgatgagaaa tcgcgtgaac tggtgacctt agcgaaaggg
    32761 cgcggattaa gcgattgtgg cattcaggcg cgctggcgct ttgacggtca gcgttttcgc
    32821 ctggtgcgtt acgccgcaga acccacctgc gataactggc atgggccaga cgcctggccc
    32881 acgttgtgga tcacccgtta aacaaaatgg cggggacatg taggccggat aagacgctgt
    32941 aggccggata agacgcgtca gcgtcgcatc cggcaatcag catccggcga tcactatcag
    33001 gcacctttca ctcccagcac cttatgcgct tttgccacaa tattctcggc ggtaaagcca
    33061 aagaacggga acagcttatc cgccggagca gattccccat aacccgtcat cccgacaatt
    33121 gcccctttca gaccaacata cttgtaccag taatcggcaa tacctgcttc caccgccacg
    33181 cgagccgcaa cgttagaagg caacaccgac tcccgatatt cctcatcctg ggcgtcgaaa
    33241 atatcggtcg agggcaggga aaccacgcga acattgcgac cttctcctgc taatttctcc
    33301 gcagcttgca gggtgatttc catctctgaa ccggtggcaa tcagaataat atctggctta
    33361 ccgccgctgt ctttcagcac ataaccgcca cgagtaatct ctttaacctg atccggcgta
    33421 cgttccacct gggccagatt ttgccttgag aggatcagtg ccgtcggtcc gttgtggcgc
    33481 tcaaccgcca gcttccagcc caccgccgct tccacctgat cgcacggtcg ccaggtgctg
    33541 aaatttggcg ttaagcgcag gctggccagt tgctcaacag cctggtgcgt cggaccatct
    33601 tcgcccaggc caattgagtc gtgggtataa accataatct gccgcgcttt catcagtgcc
    33661 gccatccgcg cggcgttacg ggcgtattca acaaacatca ggaacgtcgc ggtatacggc
    33721 acaaagccgc cgtggtgcgc gatgccgttg gcgatagcgg tcatgccaaa ttcacgcacc
    33781 ccgtagtgaa tgtagttgcc cgccggatct tccttcagcg aaacagaacc tttccagatg
    33841 gtcaggttgc tgggagccag atccgccgaa ccgccgagca gctccggtag caaggggcca
    33901 taagcgttaa gcgtattttg cgaagcctta cgggtggcga ttttcgccgg attcgcctgc
    33961 aactcattga tatacttctg agtcgttttc tcccagtcct tcggtaaacc accgctcatc
    34021 cgacgggtaa actcttctgc cagttgcgga tgagcctttt tataggcggc aaacttctca
    34081 ttccacctct gctgcgcttt ttcgcctttt tcgcgggcat cccaggcgtg ataaatatct
    34141 ttagggatct caaatggcgg atggtgccag cccagttttt gccgtgccag cgccacttct
    34201 tcttccccca gcggtgcgcc gtgcgcctct tccttacctg ctttattcgg cgatccaaag
    34261 ccaatcaccg tacgacagat aatcagcgac ggcttatctt tcacgctttg cgcttcaagg
    34321 atcgcttcct tcaccgcctg cggatcgtga ccgtcgattt catggatcac atgccagtga
    34381 taggcttcaa aacgttttgc cgtatcgtcg gtaaaccagc cttctgtttc accgtcgatg
    34441 gaaataccgt tgtgatcgta aaaaccaatc agcttgccca gtcccagcgt gcctgccagc
    34501 gaacagactt cgtgggaaat accttccatc aggcagccgt cgcccataaa cacataggtg
    34561 aagtgatcga cgatctcatg gtctggctgg ttaaactgcg ccgccagtgt acgctctgct
    34621 atcgccagcc cgacggcgtt cgccaaacct tgtccaagtg gaccggtggt ggtttcaacg
    34681 cctggcgtat aaccaatctc cgggtggcct ggggttttcg aatgcagttg acgaaagttc
    34741 ttcagctctt ccagcggcag gtcgtaaccg gtcagatgca gcaaactgta gagcagcatc
    34801 gacgcgtgac cgttggaaag aataaagcgg tcgcgatcat accaggttgg gtcggtaggg
    34861 ttatgtttaa gaaaatcgtt ccacagcact tcggcaatat cagccatgcc catcggcgcg
    34921 ccgggatgac cagaattggc tttttgtacc gcatccatac tgagtgcgcg aatcgcattg
    34981 gcaaggtctt ttcgggacat ataacactcc gtggctggtt tatagtttgg cggcaagaag
    35041 atcttccagt ttgcgttgat caacggcgaa cagacgaatg ccttcagaca gtttttctac
    35101 cgccatcgca tactgattgt gctcccaacg gaactccgct tcgctcatgg gagctgggcg
    35161 tgggaacgtc tgagaaggtg ggattaattt acgtaccact ggcgaaactt tttcctgcag
    35221 ctccttcagc aaattcggtg cgatagtcag tcgatcgcag ccggttaagg cgaggatttg
    35281 ttcggtgcga cggaagctcg cgcccatcac aatggtttca tagtggtgtt gcttatagta
    35341 gtcgtagata ttgcgcaccg atttaacgcc cggatcttct tccaccacat acgggtccat
    35401 cggcttgcgt gcctgatacc agtcataaat acgcccgaca aacggcgaaa tcagaaaaac
    35461 gcctgcttcc gcacaggccc gtgcctgtgc aaaagaaaac agcagcgtca ggttgcagtt
    35521 aataccttct ttttccagct cttctgccgc gcgaattcct tcccaggtcg aagccagctt
    35581 gatcagaatg cgtgatttct caacgccttg ttgctgatac aagtccacca gatggcgcgc
    35641 cttctcaata ctcttttctt tatcaaaaga gaggcgtgca tcaacttctg ttgacacgcg
    35701 accgggtacg attttgagga tttcagcacc gaaattgacc gccagtttgt cacacgctgc
    35761 gaccacctgt tgttcctggg tcttgccatt ttttttaccc caggcgatag catcgtctat
    35821 taaatgctca tattgtgata atccggcagc cttgagtaac agcgaaggat tggtggtggc
    35881 atcctgggga tgataatggc gaatggactc aatatcgccg ctgtctgcca cgacagtggt
    35941 gaactgtttg atgccgtcta actcgttcat aggaaatact ccttgaaaag taaagtgtta
    36001 gatgagtgcg ttaattcaca cttctgagaa atttcgctaa acgcatcaaa aaagcatagc
    36061 agacaggcat ggtattgctg gattaagcag gtaacatcag tgttatagga ttattaccaa
    36121 aacattatat taatggtgag agtttggact tgctcaaagt ctgtagactc cggcaaggta
    36181 ataatgtgcg ccacgttgtg ggcagggggt gttgcccaca cactttattt gtgaacgtta
    36241 cgtgaaagga acaaccaaat ggatgaccag ttaaaacaaa gtgcacttga tttccatgaa
    36301 tttccagttc cagggaaaat ccaggtttct ccaaccaagc ctctggcaac gcagcgcgat
    36361 ctggcgctgg cctactcacc cggcgttgcc gcaccttgtc ttgaaatcga aaaagacccg
    36421 ttaaaagcct acaaatatac cgcccgcggt aacctggtgg cggtgatctc taacggtacg
    36481 gcggtgctgg ggttaggcaa cattggcgcg ctggctggca aaccggtgat ggaaggcaag
    36541 ggcgttctgt ttaagaaatt cgccgggatt gatgtatttg acattgaagt tgatgaactc
    36601 gacccggaca aatttattga agttgtcgct gcgctcgaac cgaccttcgg cggaatcaac
    36661 ctcgaagaca ttaaagcgcc agaatgtttc tatattgaac agaaactgcg cgagcggatg
    36721 aatattccgg tattccacga cgatcagcac ggcacggcaa tcatcagcac tgccgccatc
    36781 ctcaacggct tgcgcgtggt ggagaaaaac atctccgacg tgcggatggt ggtttccggc
    36841 gcgggtgccg cagcaatcgc ctgtatgaat ctgctggtag cgctgggtct gcaaaaacat
    36901 aacatcgtgg tttgcgattc aaaaggcgtt atctatcaag gccgtgagcc aaacatggcg
    36961 gaaaccaaag ccgcatatgc ggtggtggat gacggcaaac gtaccctcga tgatgtgatt
    37021 gaaggtgcgg atattttcct gggctgttcc ggcccgaaag tgctgaccca ggaaatggtg
    37081 aagaaaatgg ctcgggcacc aatgattctg gcgctggcga atccggaacc ggaaattctg
    37141 ccgccgctgg cgaaagaagt gcgtccggat gccatcatct gcaccggtcg ttctgactat
    37201 ccaaaccagg tcaacaacgt cctttgcttc ccgttcatct tccgtggcgc gctggacgtt
    37261 ggcgcaaccg ccatcaacga agagatgaaa ctggcggcgg tacgcgcgat tgcagaactg
    37321 gcccatgcgg aacagagcga agtggtggct tcagcgtatg gcgatcagga tctgagcttt
    37381 ggtccggaat acatcattcc aaaaccgttt gatccgcgct tgatcgttaa gatcgctcct
    37441 gcggtcgcga aagcggcgat ggagtcgggc gtggcgacgc gtccgattgc tgatttcgac
    37501 gtctacatcg acaagctgac tgagttcgtt tacaaaacca acctgtttat gaagccgatt
    37561 ttctcccagg ctcgcaaagc gccgaagcgc gtagttctgc cggaagggga agaggcgcgc
    37621 gttctgcatg ccactcagga actggtaacg ctgggactgg cgaaaccgat ccttatcggt
    37681 cgtccgaacg tgatcgaaat gcgcattcag aaactgggct tgcagatcaa agcgggcgtt
    37741 gattttgaga tcgtcaataa cgaatccgat ccgcgcttta aagagtactg gaccgaatac
    37801 ttccagatca tgaagcgtcg cggcgtcact caggaacagg cgcagcgggc gctgatcagt
    37861 aacccgacag tgatcggcgc gatcatggtt cagcgtggcg aagccgatgc aatgatttgc
    37921 ggtacggtgg gtgattatca tgaacatttt agcgtggtga aaaatgtctt tggttatcgc
    37981 gatggcgttc acaccgcagg tgcaatgaac gcgctgctgc tgccgagtgg taacaccttt
    38041 attgccgata cctacgtcaa tgacgaacca gacgcagaag agctggcgga gatcaccttg
    38101 atggcggcag aaactgtccg tcgtttcggt attgaaccgc gcgttgctct gttgtcgcac
    38161 tccaactttg gttcttctga ctgcccgtcg tcgagcaaaa tgcgtcaggc gctggaactg
    38221 gtcagggaac gtgcgccaga actgatgatt gatggtgaaa tgcacggcga tgcggcgctg
    38281 gtggaagcga ttcgcaacga ccgtatgccg gacagccctt tgaaaggttc tgccaatatt
    38341 ctggtgatgc cgaacatgga agctgcccgc attagttaca acttactgcg cgtttccagc
    38401 tcggaaggtg tgactgtcgg tccggtgctg atgggcgtgg cgaaaccggt tcacgtgtta
    38461 acgccgatcg catcggtgcg tcgtatcgtc aacatggtgg cgctggccgt ggttgaagcg
    38521 caaacccaac cgctgtaatt cttttttaac tctcacgctt accctgaata ttcagggtaa
    38581 gcggtttggc tgcaatatat tagtaaagct tattactgag tttgcgaata ataaaaaaag
    38641 cagtctatat aatatctcga tattatttta tttatattca tgcgttgcat atgaaagttt
    38701 atgcaccaca gcgaatatct ctccattctt agtgatctac ctcacctttt aaacgcgctt
    38761 gccgaatttt gttatttact ctgacgaaaa attgtcacga tacacgaaag tttttcacag
    38821 gcggcgactc atggataaag aacgcatcat tcaggaattt gtgcccggca aacaggtcac
    38881 actggcgcat ctcatcgcgc atcccggtga ggaactggcg aagaaaatcg gcgttccgga
    38941 tgcgggcgta attggcatta tgacgctaac tcccggcgaa acggcgatga tcgtcggtga
    39001 tttagcgctg aaagccgctg atgtgcatat cggttttctc gaccgcttca gcggtgcatt
    39061 ggtgatttat ggctcggtgg gcgcagtaga agaagcgtta tcacaaaccg tcagcggttt
    39121 aggtcgctta ttaaattata cgctttgtga aatgaccaaa agttaatacg aggcattcat
    39181 gaaacgaatt gcgtttgtgg gtagcgtcgg ggcaggaaaa acaacgttat ttaatgcgtt
    39241 gcaggggaat tataccctcg ccagaaaaac acaggccgtg gaatttaatg ataagggcga
    39301 tattgatacg ccgggtgaat attttagcca tccccgctgg tatcacgcct taattaccac
    39361 gctacaggat gtagacatgc tgatctatgt ccatggcgca aatgacccgg aaagtcggtt
    39421 gcctgccggg ttgctggata ttggcgtcag caaacggcaa atcgccgtca tcagtaaaac
    39481 ggacatgcca gatgctgacg tcgccgcaac gcgaaagtta ttgcttgaga cgggtttcga
    39541 agagccgatt ttcgagctaa acagccacga cccgcaaagc gtgcagcaac tggtggatta
    39601 tctggcatct ctcaccaaac aggaggaagc aggtgaaaaa actcatcaca gcgaatgata
    39661 ttcgtgaagc acacgcgcgc ggcgaacagg caatgtccgt ggttctgcgc gccagcatta
    39721 ttaccccgga agcccgtaaa gtggcggatc tactgggttt taccatcacc gaatgtgatg
    39781 aatcgatccc ggtaacggcg tccgtgcccg ccagcgtgcc agccgacaaa accgaaagcc
    39841 agcgcatccg cgaaaccatc attgcccagc tgccggaagg ccagtttacc gaaagcctgg
    39901 tcgcacagct gatggaaaaa gtgatgaagg aaaaacagtc gctggagcag ggcgcaatgc
    39961 agccgagctt caaatcggtg accggcaaag gcggcatcaa agtgattgat ggcagcagcg
    40021 tcaagtttgg tcgttttgat ggcgcacaac cgcattgcgt gggcttgact gatcttgtga
    40081 ccggagacga cggcagcagc atggccgccg gttttatgca gtgggagaac gcattcttcc
    40141 cgtggaccct gaactacgac gaaatcgaca tggtgctgga aggggagctc catgttcgcc
    40201 acgaaggcga aaccatgatc gccaaagcag gcgacgtgat gtttatcccg aaaggctcca
    40261 gcatcgaatt tggtacgaca tccagcgtga aattcctgta tgtcgcctgg ccggctaact
    40321 ggcaatccct atgaaagatt tcatcaccga agcatggcta agagcgaacc atacgctcag
    40381 cgaaggcgca gagatccatc tccctgcgga cagtcgcctg acgccctctg cccgggagtt
    40441 gctggaaagc cgccatctgc gcatcaagtt tattgacgag cagggccgcc tgtttgttga
    40501 cgatgaacag cagcagccgc agcccgttca tgggctgacc agtagcgatg aacatccgca
    40561 ggcgtgctgc gaactgtgcc gccagccggt ggcaaaaaag ccggatacgc tgactcacct
    40621 gtcggcggaa aaaatggtcg ccaaaagcga tccgcgtctg ggttttcgcg cggttctcga
    40681 cagcaccatt gcgctggcag tgtggctgca aattgaactg gcggaaccgt ggcagccgtg
    40741 gctggcggat atccgttcgc gtcttggcaa cattatgcgc gccgatgcgc tgggggaacc
    40801 gctgggctgc caggcgattg tcgggctttc tgacgaagat ctgcatcgac tttctcacca
    40861 gccgctgcgc tatctcgacc acgatcatct ggtgccggaa gccagccacg ggcgcgatgc
    40921 ggcgttgctc aatctgctac gtaccaaagt gcgggaaacc gaaaccgtag cggcgcaggt
    40981 gtttatcacc cgcagctttg aagtattacg cccggacatc ttgcaggcgc tgaaccgcct
    41041 ttcgagcacg gtctacgtaa tgatgatcct gagcgtaacg aagccgccgc taacggtgaa
    41101 gcagatccaa caacgactgg gggaaacgca atgattattg aacgttgtcg tgaactggcg
    41161 ttgcgagcgc ccgccagagt ggtttttccg gatgcgttag atcaacgtgt gctgaaagct
    41221 gcgcaatatt tacatcaaca aggtctggca acgcccattc tggtcgctaa tccgtttgaa
    41281 cttcgtcagt ttgcgctcag tcacggcgtg gcgatggacg ggctacaggt gattgatccg
    41341 catggcaacc tcgcaatgcg ggaagaattt gctcatcgct ggctggcccg cgcgggcgaa
    41401 aaaacgccgc cggatgcgct ggaaaaactt accgatccgc tgatgttcgc cgcagcaatg
    41461 gtcagcgccg gtaaagcgga tgtctgtatc gcgggcaacc tctcttccac ggcgaatgtg
    41521 ctgcgtgccg gattacgtat tatcggcttg cagccaggct gtaaaacgct ctcatccatt
    41581 ttcctgatgc tgccacagta cagcggtccg gcgttgggct ttgccgattg cagcgtggtg
    41641 ccacagccga cggcggcgca actggcggat atcgcgcttg ccagtgccga aacctggcgc
    41701 gccctcaccg gagaagagcc gcgcgtggcg atgctgtcgt tttccagtaa cggtagcgcc
    41761 cgtcacccct gcgttgccaa cgtccagcag gcgacagaaa tcgtccgtga gcgcgcacca
    41821 aagctggtgg ttgatggcga gttgcagttt gacgccgcct tcgtgccgga agtggcggcg
    41881 caaaaagcgc ctgccagccc gctacagggc aaagccaatg tgatggtttt tccgtcgctg
    41941 gaagccggaa atattggcta caaaatcgca caacgactcg gcggatatcg tgccgtcggg
    42001 ccattgatac aaggacttgc cgcgccgatg cacgatctct ctcgtggttg tagcgtgcag
    42061 gaaattatcg agctggcgct ggtggcagct gtgccgcgtc agacagaagt gaaccgcgaa
    42121 agcagtttac aaacactggt tgaatgaacg gtcccgttct ggaccctgat aaagaggaaa
    42181 gcacaatgga agcattagga atgatcgaaa cccggggcct ggttgcactg attgaggctt
    42241 ctgacgcgat ggttaaagct gcgcgcgtca agctggttgg tgttaagcag attggcggcg
    42301 gcctgtgtac tgccatggtt cgtggtgatg ttgcagcctg taaagccgcg accgatgcag
    42361 gtgccgcagc ggcacagcgt atcggcgagc tggtatctgt acacgttatc ccgcgtccgc
    42421 acggcgacct ggaagaagtc ttcccgatcg gcctgaaagg cgatagcagc aacctgtaat
    42481 caccctgtgt gacgtcgatt cgggcgtcac gctctctcct ttttcatttt accttctgcg
    42541 gagggtaggg ggagctttta cctgaaatac gccacggagg cgggtatgaa actggcagtc
    42601 gtcactggac aaattgtttg taccgtacgc catcacggac tggcgcatga caaattgctg
    42661 atggtggaaa tgattgatcc acaaggtaat cctgacgggc aatgcgccgt cgccatcgac
    42721 aatattggcg cgggaaccgg ggagtgggtg ttactggtga gtggcagttc cgcccgccag
    42781 gcgcataaaa gcgaaacgtc accggtcgat ctgtgcgtga ttggcattgt cgatgaggtg
    42841 gtgtctggcg gtcaggtaat tttccacaaa taaggcagaa catcatgaat caacaggata
    42901 ttgaacaggt ggtgaaagcg gtactgctga aaatgcaaag cagtgacacg ccgcccgccg
    42961 ccgttcatga gatgggcgtt ttcgcgtccc tggatgacgc cgttgcggca gccaaagtcg
    43021 cccagcaagg gttaaaaagc gtggcaatgc gccagttagc cattgctgcc attcgtgagg
    43081 caggcgaaaa acacgccaga gatttagcgg aacttgccgt cagtgaaacc ggcatgggcc
    43141 gcgttgaaga taaatttgcc aaaaacgtcg ctcaggcgcg cggcacgcca ggcgttgagt
    43201 gtctctctcc gcaggtgctg accggcgaca acggcctgac tttaattgaa aacgcgccct
    43261 ggggcgtggt ggcttcggta actccgtcca ctaacccggc ggcaaccgta attaataacg
    43321 ccatcagcct gattgccgcg ggcaacagcg tcatttttgc cccgcatccg gcggcgaaaa
    43381 aagtctccca gcgggcgatc acgctgctca accaggcgat tgttgccgca ggcgggccgg
    43441 aaaacttact ggttactgtg gcaaatccgg atatcgaaac cgcgcagcgt ttgttcaagt
    43501 ttccgggcat cggccttctg gtggtgaccg gcggcgaagc ggtagtagaa gcggcgcgta
    43561 aacacaccaa taaacgtctg attgccgcag gcgctggcaa cccgccggta gtggtggatg
    43621 aaaccgccga cctcgcccgt gccgctcagt ccatcgtcaa aggcgcttct ttcgataaca
    43681 acatcatttg tgccgacgaa aaggtactga ttgttgttga tagcgtagcc gatgaactga
    43741 tgcgtctgat ggaaggccag cacgcggtga aactgaccgc agaacaggcg cagcagctgc
    43801 aaccggtgtt gctgaaaaat atcgacgagc gcggaaaagg caccgtcagc cgtgactggg
    43861 ttggtcgcga tgcggcgaaa atcgcggcgg caatcggcct gaacgtgccg caagaaacgc
    43921 gtttgctgtt tgtggaaacc actgcagaac atccgtttgc cgtgaccgaa ctgatgatgc
    43981 cggtgctgcc cgtcgtgcgc gtcgccaacg tggcggatgc cattgcgctg gcggtgaaac
    44041 tggaaggcgg ttgccaccac acggcggcaa tgcactcgcg caacatcgaa aacatgaacc
    44101 agatggcgaa tgctattgat accagcattt tcgttaagaa cggaccgcgc attgccgggc
    44161 tggggctggg cggggaaggc tggaccacca tgaccatcac cacgccaacc ggtgaagggg
    44221 taactagcgt gcgtacgttt gtccgtctgc gtcgctgtgt attagtcgat gcgtttcgca
    44281 ttgtttaagg agtaacagat ggcgcacgac gaacaatggc tcaccccacg tctgcaaacg
    44341 gcggcaacgc tgtgtaacca gacgcccgcc gcgacggaat ctccgctgtg gctgggggtg
    44401 gatctgggca cctgcgatgt ggtgtcgatg gttgtcgacc gcgacggtca gtcggtggcg
    44461 gtatgcctcg actgggccga tgtggtccgc gacggcatcg tctgggattt cttcggcgct
    44521 gtcaccattg ttcgtcgcca tctcgacacg ctcgagcagc aattcggtcg ccgttttagc
    44581 catgcggcga cttcatttcc tccaggtacg gacccgcgta tttccatcaa cgtgctggag
    44641 tctgctggac tggaagtgag ccatgtgctg gatgagccaa ccgcagttgc ggatctgctg
    44701 caactggaca acgccggtgt agtggatatc ggcggcggca ctaccggcat tgccatcgtg
    44761 aaaaagggca aggtgacgta ctcggcggat gaagcaaccg gcggacatca catctctctg
    44821 actctcgccg gaaatcgccg tatttcgctg gaagaggcgg agcagtacaa gcgcggtcac
    44881 ggtgaagaga tttggcctac ggtgaaaccg gtgtacgaaa aaatggtgga cattgtcgcc
    44941 cggcatattg aagggcaggg cattactgat ttatggctgg cgggcggctc ttgtatgcaa
    45001 ccgggcgtgg cggaactgtt tcgcaaacaa ttcccggcgt tacaggtgca tttaccgcag
    45061 cacagtttgt ttatgacccc gctggcgatc gccagtagtg ggcgagagaa agcggagggg
    45121 ctatatgcaa aatgaattgc agaccgcgct ctttcaggcg ttcgataccc tgaatctgca
    45181 acgggtaaaa acatttagcg ttccaccggt gacgctttgc ggtccgggct cggtgagcag
    45241 ttgcggacag caagcgcaaa cgcgtgggct gaaacatctg ttcgtgatgg cagacagctt
    45301 tttgcatcag gcagggatga ccgccgggct gacgcgcagc ctggctgtta aaggcatcgc
    45361 catgacgctc tggccatgtc cggtgggcga accgtgcatc accgacgtgt gtgcagccgt
    45421 ggcgcagttg cgtgagtcag gctgtgatgg ggtgatcgca tttggcggcg gctcggtgct
    45481 ggatgcggcg aaagccgtgg cgttgctggt gacgaacccg gatagcacgc tggcagagat
    45541 gtcagaaacc agcgttctgc aaccgcgctt gccgctgatt gccattccaa cgaccgccgg
    45601 aaccggctct gaaaccacca atgtaacggt gattatcgac gcggtgagcg ggcgcaagca
    45661 ggtgttagcc catgcctcgc tgatgccgga tgtggcgatt ctcgacgccg cgttgaccga
    45721 aggtgtgccg tcgcatgtca cggcgatgac cggcattgat gcgttaaccc atgccattga
    45781 agcatacagc gccctgaacg ctacaccgtt taccgacagt ctggcgattg gtgccattgc
    45841 gatgattggc aaatcgctgc cgaaagcggt gggctacggt cacgaccttg ccgcgcgcga
    45901 gagcatgttg ctggcttcat gtatggcggg aatggcgttt tccagtgcgg gtcttgggtt
    45961 gtgccacgcg atggcgcatc agccgggcgc ggcgctgcat attccacacg gtctggcgaa
    46021 cgccatgttg ctgccaacgg tgatggaatt taaccggatg gtttgtcgtg aacgctttag
    46081 tcagattggt cgggcactgc gaactaaaaa atccgacgat cgtgacgcta ttaacgcggt
    46141 aagtgagctg attgcggaag ttgggattgg taaacgactg ggcgatgttg gtgcgacatc
    46201 tgcgcattac ggcgcatggg cgcaggccgc gctggaagat atttgtctgc gcagtaaccc
    46261 gcgtaccgcc agcctggaac agattgtcgg actgtacgcg gcggcgcaat aaatgccgga
    46321 tgcgacgctt gccgcgtctt atccagccta cgggattgca catgtagggc ggataaggcg
    46381 ttcacgccgc atccgccagt ggcgcggtgc aattgccgga tgcgatgctt gccgcgtctt
    46441 atccggccta cgggattgca catgtagggc ggataaggcg ttcacgccgc atccgccagt
    46501 ggcgcggtgc aattgccgga tgcgacgctt gccgcgtctg atccggcctt cgggattgca
    46561 catgtagggc ggataaggcg tttacgccgc atccgccaat aaataacaac taacagggag
    46621 taaaggcgat gggaattaac gaaatcatca tgtacatcat gatgttcttt atgctgatag
    46681 ctgccgtaga caggatcctg tcgcagttcg gcggttctgc tcgtttcctc ggtaagttcg
    46741 gtaaaagtat cgaaggatca ggcggtcagt tcgaagaagg ctttatggca atgggcgctc
    46801 tgggtctggc gatggtcggt atgaccgcgc tggcaccggt actggctcac gtactcgggc
    46861 cggtaattat cccggtttac gaaatgctcg gcgcaaaccc gtcaatgttc gccggaacgc
    46921 tgctggcgtg cgatatgggc ggcttcttcc tcgccaaaga gctggcgggc ggcgacgtag
    46981 cagcgtggct atactctggg ttaattcttg ggtcgatgat ggggccaacg attgtgtttt
    47041 ccattccggt ggcgctcggc attatcgaac cttctgaccg tcgttatctg gcgctcggcg
    47101 tgctggcggg cattgtgacc attccgattg gctgtattgc tggtggtctg gttgctatgt
    47161 actccggtgt gcagatcaac ggtcagccgg tggaattcac tttcgccctg atcctgatga
    47221 acatgatccc ggtgatcatt gttgcgattc tggtggcgct ggggctgaaa ttcatcccgg
    47281 aaaaaatgat caacggcttc cagatcttcg ccaaattcct cgttgcattg atcaccctcg
    47341 gtcttgctgc cgcggtagtt aagtttctgc ttggctggga actgatcccc ggtctggacc
    47401 ctatctttat ggcccctggc gataaacccg gtgaagtgat gcgcgccatt gaagttatcg
    47461 gttctatctc ctgcgttctg ttaggggcgt atccaatggt gctgctgctg acccgctggt
    47521 ttgaaaaacc gctgatgagc gtcggtaaag tgctgaatat gaacaacatc gcggcagccg
    47581 gcatggtggc aacgcttgcc aacaacatcc cgatgttcgg catgatgaag cagatggata
    47641 cccgcggcaa agtcatcaac tgcgccttcg ccgtttccgc tgctttcgcc ctgggcgacc
    47701 acttaggctt cgccgctgcc aacatgaacg ccatgatctt cccgatgatt gtcggcaagt
    47761 tgatcggcgg cgtaacggcg attggcgtgg cgatgatgct ggtgccaaaa gaagacgcga
    47821 ccacggctaa aaccgaagcg gaggcacaat cgtgaacact cgccagctat tgagcgtcgg
    47881 tatcgatatc ggcaccacca ccacccaggt gattttctcc cacctggagc tggttaaccg
    47941 tgcggcggtg tcgcaggtgc cgcgctacga attcattaaa cgcgaaatta gctggcaaag
    48001 cccggtgttc tttacccctg tcgataaaca gggtggttta aaagaagcgg aactgaaaac
    48061 cttaatcctc gagcaatatc aggctgcggg cattgcgccg gaaagcgttg attctggtgc
    48121 catcatcatc accggtgaaa gcgcgaaaac ccgcaatgct cgtccggcgg tgatggtgct
    48181 ctcccagtcg ctgggcgatt ttgtcgttgc cagcgccggg ccgcacctcg aatccgtcat
    48241 tgccggtcac ggtgccgggg cacaaaccct ttctgaacaa cggctgtgtc gggtactgaa
    48301 tattgacatc ggcggtggca ctgcgaacta cgccctgttc gatgccggaa aaatcagcgg
    48361 cactgcctgc ctcaacgtcg gtggtcgcct gctggaaacc gacagccagg ggcgcgtggt
    48421 ttacgctcat aaaccggggc agatgattgt ggatgagtgc ttcggcgcag gcaccgacgc
    48481 ccgttcgctg accggcgcgc agctggtgca ggttacccgg cggatggcag aactgattgt
    48541 cgaagtgatt gacggaacgc tctcgccgct cgcgcaggca ttgatgcaaa ccggtttgct
    48601 gcccgcaggt gttacgcccg aaatcattac gctttctggc ggcgtgggcg aatgttatcg
    48661 ccaccagccc gccgacccgt tctgttttgc cgatattggc ccgctgctgg caacggcgct
    48721 gcatgaccat ccgcgcctgc gtgagatgaa cgtacagttt ccggcgcaaa ccgtgcgcgc
    48781 cacggtgatt ggcgcgggcg cgcataccct ttcgctctct ggcagcacaa tctggcttga
    48841 gggcgtacaa ctgccgctgc gcaatttgcc ggtggcgatc ccgattgatg aaacggatct
    48901 ggtgagtgcc tggcaacagg cgctgcttca gctggatctt gatcccaaaa ctgacgcgta
    48961 cgtgctggcg cttcccgcct cgctgcctgt gcgttacgcc gcggtactga cggtcatcaa
    49021 cgcgctggtc gatttcgtcg cgcgttttcc aaatccgcat cccctgctgg tggtggccgg
    49081 gcaggacttt ggtaaagctc tgggcatgtt gttgcgccca cagctacaac aactcccgtt
    49141 ggcagtcatt gacgaagtga ttgtccgcgc gggggactat atcgacattg gtacgcctct
    49201 ttttggcgga tcggttgtgc cggtgacggt gaaatcactc gcatttcctt cctgagggaa
    49261 cgacttatga aactaaagac cacattgttc ggcaatgtat atcagtttaa ggatgtaaaa
    49321 gaggtgctgg ctaaagccaa cgaactgcgt tcgggggatg tgctggcggg cgttgcagcg
    49381 gcaagctcac aggagcgcgt ggcggcaaag caggtgttgt cggaaatgac cgtagcggac
    49441 atccgcaata atccggtgat tgcctatgaa gatgactgcg tgacgcggct gattcaggac
    49501 gatgttaacg aaacggccta caaccagatt aaaaacagga gcatcagcga actgcgtgag
    49561 tatgtgctga gcgatgaaac cagcgtggac gacattgcct ttacccgcaa agggctgacc
    49621 tcggaagtgg tcgcggcggt agcgaagatt tgctccaacg cggacctgat ctacggcgcg
    49681 aagaaaatgc cggtaatcaa aaaggccaat accaccatcg gtattccggg caccttcagc
    49741 gcccgtttgc agccaaatga cacccgtgac gacgtgcaaa gtatcgctgc acaaatctac
    49801 gaagggcttt ccttcggggt gggcgatgcg gtgatcggcg ttaacccggt aactgacgac
    49861 gtggaaaact taagccgcgt gttggacacc atttacggcg tgatcgacaa attcaacatc
    49921 ccaactcagg gctgcgtact ggcgcacgtc accacccaga tcgaagcgat tcgtcgcggc
    49981 gcgcctggcg gactgatttt ccagagtatc tgtggcagcg aaaaagggct gaaagagttt
    50041 ggcgtggaac tggcgatgct cgacgaagcg cgcgcagtgg gtgctgagtt caaccgtatc
    50101 gccggggaaa actgcctcta cttcgaaacc gggcaaggtt cggcgctgtc tgctggcgct
    50161 aacttcggcg cagactaggt gacgatggaa gcacgtaact atgggctggc gcgtcattac
    50221 gatccgttta tcgtcaacac cgtggtcggc tttattgggc cggagtatct ctacaacgac
    50281 cgccagatta tccgtgctgg cttagaagat cactttatgg gcaagctgag cggcatctct
    50341 atgggctgtg actgctgtta caccaaccac gctgatgctg accagaacct caacgaaaac
    50401 ctgatgatcc tgctcgccac cgcaggctgc aactacatca tggggatgcc gctgggcgat
    50461 gacatcatgc tcaactatca gaccaccgca ttccacgaca ctgccactgt gcgtcagtta
    50521 ctcaacctgc gcccgtcacc ggagtttgaa cgctggctgg aaagcatggg cattatggca
    50581 aacggtcgcc tgaccaaacg ggcgggcgat ccgtcactgt tcttctgatg acgcggagat
    50641 aacacatcat ggatcaaaaa cagattgaag aaattgtacg cagcgtgatg gcgtcaatgg
    50701 gacaagcggc ccccgcgccg tcagaagcaa agtgcgccac cactaactgt gcggcaccgg
    50761 tgacctcgga aagctgcgcg ctggatttag gttctgcgga agcaaaagcg tggattggtg
    50821 ttgaaaaccc gcatcgcgca gacgtattaa cagaactgcg ccgcagcacc gtggcccgcg
    50881 tttgtaccgg tcgtaccggt ccgcgtccac gtacgcaggc gctgctgcgt ttcctggccg
    50941 atcactctcg ttcgaaagat accgtactga aagaagtgcc ggaagagtgg gtgaaagcgc
    51001 agggcttgct ggaagtacgc tccgaaatta gcgacaaaaa tctctacctg actcgcccgg
    51061 atatgggccg ccgcctgtgt gcagaagctg ttgaagcgct gaaagcacag tgtgttgcta
    51121 atccggacgt acaggtcgtt atttctgatg gcctgtcaac tgatgccatc accgtgaact
    51181 acgaagagat cctgccgccg ctgatggcgg gcctgaaaca ggccgggctg aaagtcggca
    51241 cgccattctt tgttcgttat ggtcgcgtga agattgaaga tcagatcggt gagatcctcg
    51301 gcgcgaaagt ggtgatcctg ctggtggggg aacgtccggg gttgggtcag tcagaaagcc
    51361 tctcctgtta cgccgtttac tcgccgcgta tggcgaccac cgttgaggcc gatcgcacct
    51421 gcatctctaa cattcaccag ggcggcacgc cgccggttga agccgcagcc gtcattgtgg
    51481 atttggcgaa acgtatgctg gagcagaaag catccggcat caacatgacc cgataaggag
    51541 gcatcatgcc agctttagat ttgattcgac cgtcggtaac cgctatgcgg gtgattgcct
    51601 ccgttaacgc tgaatttgcg cgtgaactaa aattgccgcc gcatattcgt agcctcgggc
    51661 tgatttctgc tgattccgat gacgtcacct acattgcggc tgacgaagcg accaagcagg
    51721 cgatggtcga agtggtatat ggccgctcgc tgtatgcggg agcagcacac ggaccgtcac
    51781 cgactgccgg tgaagtgctg attatgcttg gtgggccaaa cccggcggaa gtgcgtgctg
    51841 gtctggatgc gatggttgcg catattgaaa atggcgctgc tttccagtgg gctaatgacg
    51901 cagaaaacac cgcattcctg gcacatgtag tttcgcgtac cggttcttat ctttcatcaa
    51961 ccgccgggat cacgcttggc gatccgatgg cgtatctggt ggcaccgccg ctggaagcga
    52021 cctacggcat tgatgcagcg ttgaaatctg ccgacgtaca gctggtgacc tatgtcccgc
    52081 caccgtctga aaccaactac tcggcagcat ttttaaccgg tagccaggcc gcgtgtaaag
    52141 cagcctgtaa cgcctttacc gatgcagtgc tggaaatcgc gcgtaatcca atccagcgtg
    52201 cgtaacggag gttgccgatg atcaatgcac tgggattgct ggaagtggac ggcatggtcg
    52261 ccgcgataga tgcagcggat gccatgcaag ccccattaac ctgtcggagg tgccgggtgt
    52321 cctacaacac ccggcattaa catcatgaaa aagacccgta cagccaattt gcaccatctt
    52381 tatcatgaac ccttacccga aaacctgaag ctcacgccga aggtcgaagt ggataatgtt
    52441 catcaacgac agacaacgga tgtctatgaa catgctttga cgattaccgc ctggcagcag
    52501 atttacgatc agctgcatcc gggcaagttt catggtgaat ttacggaaat tctgctcgat
    52561 gatattcagg tttttcgtga atacaccggt ctggcgctgc gtcagtcgtg cctggtctgg
    52621 ccgaactcgt tctggtttgg cattccggcg acgcgcggtg agcagggatt tatcggttcg
    52681 caatgtctgg gaagcgcaga aatagcgacg cgccccggtg gaaccgagtt tgaattaagt
    52741 acgccggacg attatacgat cctgggcgta gtgctgtctg aagatgtcat cacccgtcag
    52801 gctaactttt tgcacaatcc ggatcgggta ttacatatgc tgcgtagcca gtcggcgctg
    52861 gaagtgaaag agcagcataa agccgcgctg tggggctttg tccaacaggc gctggcgacg
    52921 ttttgcgaga acccggaaaa tctccatcag cctgcagtgc gaaaagtgct gggggataat
    52981 ttgctaatgg cgatgggggc gatgctggaa gacgcgcaac caatggtgac ggcggaaagc
    53041 atcagtcatc agagttaccg tagattactt tcccgcgccc gtgaatatgt gctggaaaat
    53101 atgtctgagc tggtgacggt gctggacttg tgtaatcaac tgcatgtcag tcgccgcacg
    53161 ctacaaaacg cgtttcacgc tattttaggc attggcccaa acgcgtggct gaaacgcatt
    53221 cgcctgaacg ccgtacgccg cgaactgata agcccgtggt cgcaaagcac aacggtaaaa
    53281 gacgccgcca tgcagtgggg attctggcat ctggggcaat ttgccacgga ttaccagcag
    53341 ctgtttgccg agaagccgtc gttgacgttg catcagcgga tgcgggaatg ggggtgaggg
    53401 agttacaccc aatccctgac cttaataaac tcacttaacg ccgcttctgg gctgccatct
    53461 tttggctgat aatcatactc ccagcgtacc agtggcggca ttgacatcag gatcgactcg
    53521 gtgcgcccgc cagtttgcag gccaaacaga gtgccgcgat cccagaccag attgaactcg
    53581 acataacgac cgcgacggta gagctgaaaa ttgcgctcgc gctcgccgta ggccatcgct
    53641 ttacgtcgct ctacaattgg taaataagcg tcggtgtagc ctttgcctac cgcctgcata
    53701 aaggcaaaac agtggtcgaa atctggcgtg ttcagatcat caaagaacag cccgccaata
    53761 ccgcgctgtt cgttgcgatg tttgaggtag aagtaatcgt cgcaccactt tttgtaacgg
    53821 ggataaacgt cttcaccaaa tggcaggcac aggtcacggg cggtgcggtg ccagtgaatg
    53881 gcgtcttctt caaaaccata gaaaggggtt aaatcgaagc cgccgccaaa ccaccagacg
    53941 ggctctgcac ccggtttttc ggcaataaaa aaacgcacat tcgcgtggct ggtgggaaca
    54001 tacgggttat gcggatgcac taccagtgaa acgcccatcg cctcgaaact gcgcccggca
    54061 agttccgggc gatgagcggt ggcggaagca ggcatcgcct cgccgtggac gtgagaaaag
    54121 ttgacgcctg cctgttcgaa aacaccacca ttacgcaaca cccgactacg cccgccgccg
    54181 ccagcttcgc gctgccaact atcttcgaca aattctgcgc catcgacggc ggtcagctgc
    54241 tgacaaatcg tatcctgaag gttgagcaga aactgtttaa cctggtgtgc gtcgggtttc
    54301 ataacttatc gctttttcga atgtgctttc tggttgtcga accagtggaa ataactgatc
    54361 acgccttcag caatcgctgt ggcgattttc tgacgaaacg ccgccgtgcc taacagccgc
    54421 tcttcttccg ggttggtgat aaacgaggtt tccaccagca ccgaaggaac cgacggcgat
    54481 ttcaacacca caaacgcggc ttgttcggtg ttgcggctgt gcagtttatg caccggctta
    54541 atctttttca gaatatgcga gccaagcgtc aggctgtttt taatagtatc ggtttgcacc
    54601 agatcaaaca gcacttgctg caataggtga tccttgtcag tcgccttttt accggcaact
    54661 tcatcggcgc ggttttcgcg ttcagacagg tatttcgcca ttgcgctact tgccccacgg
    54721 ttagagaggg caaataccga agcaccggca gctttcgggt tggtaaagcc atcggcatga
    54781 attgacataa acagatctgc gccatgttta tgggcgattt caacgcgatc gtaaagtggg
    54841 ataaacgtat cgccagaacg cgttaaacgc gcatcaatcc catgattacg caaaatggaa
    54901 cggacgtttt tagcaatcgc cagcaccaca tgtttttctt tcgaaccgtt gcgtccgatc
    54961 gctccggtat caattccgcc gtggcctgga tcgagaacaa cgacacgttt gccgccagat
    55021 tttttggctt tcggcttgct gtgtccgttg ctggttttta aaggttcgtc tttggcgatg
    55081 gcttgcgaca ttcctgacaa cgtcagggca gccaaaccgg ctttcagcac ctggcggcgc
    55141 gaagtgagtg tttttagtgg tttaaaagtg ctcatacggc ctgagttgta ataataaagt
    55201 tccagatgtt atatcgtatc gcgtaatccg ttacgacggt ttgatgttga gaattgtttt
    55261 acttttcatt tcaatagttg acaactcccc attatgccac tttttcaacc gattttcgtc
    55321 agatattggc taattcgacc gttcacgcca taatcagcct tttaacccgc aaaaaggtaa
    55381 cgaataccat ggagatacgc gtatttcgcc aggaagattt cgaagaggtc atcacccttt
    55441 gggagcgttg cgacttgctg cgtccgtgga acgatccgga aatggacatc gagcgtaaga
    55501 tgaaccatga cgtcagtttg tttttggtcg ctgaggtaaa cggtgaagtg gtcggaacgg
    55561 tgatgggcgg ttatgacggg catcgcgggt ctgcttatta tcttggcgtg catccagagt
    55621 ttcgtgggcg tgggattgcc aatgcgttgc ttaatcggct ggagaaaaag ctgattgctc
    55681 gtggctgccc gaaaattcag atcaatgtgc cggaagataa cgacatggtg ctcggaatgt
    55741 atgaacgcct gggatatgaa cacgccgacg tgctgagtct gggtaagcgt ttgattgaag
    55801 atgaagagta ctgagttcca tcctgtccat tatgatgcgc acggacgcct gcgtttaccc
    55861 ttgctcttct ggcttgtgct attgcttcag gcgcgaacct gggtgctgtt tgtcattgcc
    55921 ggtgcgtcgc gcgagcaggg tactgcgctg ctaaatctgt tttatcccga tcacgataat
    55981 ttctggctgg ggctgattcc aggcattccg gcggtgctgg cgtttttgtt gagcggtcgg
    56041 cgggctacat ttcctcgcac ttggcatgtg ctctattttc tgttgctgtt ggcgcaggtg
    56101 gttttacttt gctggcaacc gtggctgtgg ctgaacggtg aatccgttag cggtatcggt
    56161 ctggcgctgg tagtggcgga tattgtggcg ttaatctggc tgctgaccaa tcgacgttta
    56221 cgcgcttgtt ttaatgaaga aaaagaataa cggcactttt tggtgaattt gcactccaag
    56281 caacgttatt gaataaccaa aggaagtgac atgaaatcgc tgcgtttaat gttatgcgct
    56341 atgccgttga tgctgaccgg ctgttccacg atgtcgtcag ttaactggtc ggccgctaac
    56401 ccgtggaact ggtttggatc gtccaccaaa gtgagcgagc agggcgtggg tgaattaacg
    56461 gcgtccacac cactgcaaga acaagccatt gctgatgcgc ttgatggcga ttatcgcctg
    56521 cgcagcggaa tgaaaaccgc gaacggcaac gtggtgcgct tttttgaagt gatgaaaggc
    56581 gacaacgtgg cgatggtgat taacggcgat caaggcacga tcagtcgcat tgacgtgctg
    56641 gatagcgata ttcctgctga caccggtgtt aaaatcggta caccgtttag cgacctttac
    56701 agcaaagcat ttggcaattg ccaaaaagct gatggtgatg ataatcgtgc cgtcgaatgt
    56761 aaagccgaag gcagtcaaca tattagctac cagttcagcg gggaatggcg tggtcctgaa
    56821 gggttaatgc cttcggacga taccctgaaa aactggaaag tcagtaaaat tatctggcgg
    56881 cgttaatttg cgtctgaaca aaccgccgcc gcgaaaaaac acgtaaaata gcgctcaaca
    56941 atgccacgga ttgcgtggca ttcttatttt caggaggaac aatgtctcag gttcagagtg
    57001 gcattttgcc agaacattgc cgcgcggcga tttggatcga agccaacgtg aaaggggaag
    57061 ttgacgccct gcgtgcggcc agtaaaacat ttgccgacaa actggcaact tttgaagcga
    57121 aattcccgga cgcgcatctt ggtgcggtgg ttgcctttgg taacaacacc tggcgcgctc
    57181 tgagcggcgg cgttggggca gaagagctga aagattttcc gggctacggt aaaggccttg
    57241 cgccgaccac ccagttcgat gtgttgatcc acattctttc tctgcgtcac gacgtaaact
    57301 tctctgtcgc ccaggcggcg atggaagcct ttggtgactg cattgaagtg aaagaagaga
    57361 tccacggctt ccgttgggtg gaagagcgtg acctgagcgg ctttgttgac ggtacagaaa
    57421 acccggcggg tgaagagacg cgtcgcgaag tggcggttat caaagacggc gtggatgccg
    57481 gcggcagcta tgtgtttgtg cagcgttggg agcacaacct gaagcagctc aaccggatga
    57541 gcgttcacga tcaggagatg atgatcgggc gcaccaaaga ggccaacgaa gagattgacg
    57601 gcgacgaacg tccggaaacc tctcacctca cccgcgttga tctgaaagaa gatggcaaag
    57661 ggctgaagat tgttcgccag agcctgccgt acggcaccgc cagtggcact cacggtctgt
    57721 acttctgcgc ctactgcgcg cgtctgcata acattgagca gcaactgctg agcatgtttg
    57781 gcgataccga tggtaagcgt gatgcgatgt tgcgtttcac caaaccggta accggcggct
    57841 attacttcgc gccgtcgctg gacaagttaa tggcgttgta agcgtttctg attcgatgat
    57901 taaaggccag ccagtaacaa aactgactgg cctttttatt acttttgctt caacgccgca
    57961 tacacctgat caactaccca accataagtt gcaatcggta actgaccgct ttcgaacata
    58021 ttgggatttt tttgtggatc ggcaactggc gaatggggct tgttgcttaa catcacaatc
    58081 gccatatgat tgaccggatc gataacggtt actgtcccgg tccagccagt gtgaccgtag
    58141 gtttgcgggc ttgccagcgt gccaaacgtc ggcgtcatgg tggcattacc attcacgcgc
    58201 cagccgaggc caaaagtggc atcttcctta gagctggtgg tgaacatctt caccgtttcc
    58261 gcactgaaca gctgcacatc gccatagccc ccgccgttca gcatcgtttg cattaacacc
    58321 gcaatatcgc cggtattgga aaacaaacct gcgtgcccgg aaacgccgcc catcgaataa
    58381 aaggcttttt catcgtgcac ctgaccccag agagtggagg tgcggatatt cgggaaatgg
    58441 atcacgccat cgcgggtatt gccgttcaat tccgtggcgg caatttgttg cggtttaaag
    58501 cctttcagca gcgggttaaa taccgtatgt gtcaggccga gcgggcgata aatcgactct
    58561 tcaacatagc ggtcaagcgg ctgaccggta accgactcga cgataaatcc aagcagcata
    58621 taatcgacat cgctgtagat atgtttgctg ccgggctgat attccagcgg cgtgcgcttg
    58681 atcatctcca gcgtctggcc tttatcctgg gaatataacg cgcccgcgac cgctttattc
    58741 gggtattgcg gatctgccgg gaaaccgccg ctgtgatgca gcaggtcaga aatccgcagg
    58801 gtgtttttgc ctttaatggt gtcatttgga ctatcggcaa accccggaat atatttcgca
    58861 atccgatcgt caggatgcag tttgccttcg ctcatcagct tttgcagggc gaagttcgtg
    58921 gcgtacattt tggtgtttga ggccaggtca tacagcgtcc cggtggtggc tttgacaggc
    58981 tgttccatca acacgctgcc atcgtacttt ttcgccgctc cccaggcttt gcgatacaca
    59041 atctgattat ctttaatgat cagcaggttt acgccgggat aaccgacatc aatttgctgg
    59101 ctaatccagc gatccatctg gttaagccgt tcgacgttaa accctgcttt ctctggcgag
    59161 ctttctgtca gaacagggta ttttgcggcg ctgacgctac aactaacagc cagcagagaa
    59221 agatagagca ttgtccgttt catgatatca gtccagattg acgttacggc agccaaagag
    59281 cgtggtgaaa ataaacccgc aaacccatgc caccagaatc ccgccagcat aaaccgccat
    59341 ggccggaagg atgccttgtg cggaagtcat cagcggcagc gctaccagac cagacggccc
    59401 aaaggcgctg tttaagccca tcggcagacc ccatcaggct atcaggccga taaacaaacc
    59461 gcccgccgcg ccgcctaaac aggcggtaac aaacggcttc atgcggggca gggtgacacc
    59521 gtaaatcagc ggttcaccaa cgcccagcag gccgggaata atcgccccgc gtacctgact
    59581 gcgtagcgca ctgtgcggtt gcgcccgcca gtagagtgcc agcgccgcgc ccacctggcc
    59641 cgcgcctgcc attgaaagga tgggaaataa gctgttgaat ccctagctgt ccattaacgc
    59701 gaggtaaaca ggaataaagc cctgatgcac gccaaatacc acggcgatca ggaacagccc
    59761 ggctaaaacc gcacaaccga acggattact gttcaggtgc ataaacagcc atgacatacc
    59821 ttcgaataac cagccaccca gcggcataat gatcaggtac gcaagcgtag cggtgatcag
    59881 cagggtgatt aacgaggtca gcagcatgtc gagatcgtcc gacataaagc ggcgcaccat
    59941 gccttcaatg cgggcgcagg cccacgcggc aatcagcacg ccgataatat tgccgcgcgg
    60001 atcgatgggc agaccaaaga aatcgtgaaa accggcgtag tcaccggtgg ttgcagcggg
    60061 gttataaccg agcaaaaaga gtgcggcaat aattgcgcca ttgacgcccg tgccgccaaa
    60121 tgcctgggcg gcgttatagc ccaccagaat caccaggaaa gtgaacaaac ctttgctgaa
    60181 caccttcata aaattcagcg cgtcgggtag cgtgccctga gcatctgccg gaacgtgcat
    60241 caccgtggca attaacgtcg ctatccccag cagcagaccg gcggcaataa aaccggggat
    60301 cagcggcgtg aagatggtgg cgaatttggc gagaaattgt tgcacgccgg aggtttgttt
    60361 ggcttttaac tgacgtttgt tctgggcggc gatttctgcg gcatcctgta ccggtgcctc
    60421 gcccagcagc tcgctcatcg cttttgcggc acgatgcgct ttaccaggcc caaaaacgac
    60481 ctgcacctgg tcgctggtca aaatgacgcc cttcacgcct tccagcgttt tgatattggg
    60541 atcaaccagt gaactgtcat gtacacccag acgcaggcgc gtcatacagt taccacaact
    60601 ggcgatattt cccagtccgc cgacacgggt aagaatggtg ttcagaagtt cactgctgat
    60661 ctctttggcc atgggttatt ccttgtctaa aacctgacga ataaagccgc cgtgttgatc
    60721 caggcgtttt ttagcttctg cggcatcgag atttttcagc accatcacaa tggccgtttt
    60781 acagttgcgc tcgcaggcaa ttaacgccgc ttccgcttgt tcggcattac atccggtggc
    60841 gtttttaacg atattgacct ggcgcacatg cagtttttcg ttggtggcga ccacatcgac
    60901 catcaggttg ccgaacactt tgccggattt aatcatcagc ccggtggaaa gcatattgag
    60961 caccagtttc tgcgctgtgc ctgctttcat tcgcgaagaa ccagtgacca cttcggcacc
    61021 tacaatcggt gtaatggcaa actcggcggt ggtggaaacg gcactccccg gattacagga
    61081 aattcccact gtgcggcaac cgagctggcg ggcgtattcc agtccggcaa tcacatacgg
    61141 cgtacgaccg ctggcggcaa taccgaccac cacatcctgt gccgttaaat ttatattttt
    61201 cagatcgtta atgccgcctt ctcggctatc ttccgtgcct tccaccgcgt gctgaatggc
    61261 atattcgccg ccagcaatca aaccaaccac cagacccggt ttcacgccgt aggtgggcgg
    61321 acattcgctg gcatccagaa tccccagacg accggatgtt cccgcaccga gataaatcag
    61381 acgaccgccg ccgcttacct gggcgtggat aacatcgatc gccgcggcga tatccggcag
    61441 gacgcgctca acggcaagcg gtacggtttt atcttcatcg ttgataatcc ggcacatttc
    61501 cagcgtcgat acgcggtcaa tttcagccga ggcggtgttc gagccttcag taatcatctt
    61561 ttcaagttgc atcggtggtg accttactat ttacgatcaa ataattgcag atgtatggaa
    61621 tcatatatcc cgaattgaca gacttaaagg aatcattgag tctacacttg cagagtgtga
    61681 agagcgtcat aagaacttaa gcggaatttt tgctgccagc ccgggagagt ggaagatgtt
    61741 gtacctgacg aaaatacgca atgcggaaag tgaatttacc gagaacgagc aaaaaattgc
    61801 tgactttctg cgggccaacg tgagtgagct gaaatccgtc tcttcgcgca agatggcgaa
    61861 acagctcggc atcagtcagt cgagcattgt gaaatttgcc cagaagctcg gcgcgcaagg
    61921 atttactgaa ttgcgtatgg cgttaattgg cgaatacagt gccagccggg agaaaacgaa
    61981 tgccacggcg ctgcacctgc acagttcgat taccagtgat gattcgctgg aagtgattgc
    62041 ccgcaaactg aatcgcgaaa aagagctggc gctggagcaa acctgtgcgt tgttcgacta
    62101 cgctcggctg caaaaaatca ttgaggtcat cagtaaagca ccgtttatcc agataacggg
    62161 cctgggcggg tcggcgctgg tggggcgcga tctgtcgttc aaactgatga aaattggcta
    62221 tcgcgtggcc tatgaagccg acacacacgt acaagccacc gtttcccagg cgttgaaaaa
    62281 aggcgatgta caaatcgcta tctcttacag cggcagtaaa aaagagattg tcttgtgcgc
    62341 agaagcggca cgaaagcagg gggcaacggt gattgccatt acttctctgg ctgactctcc
    62401 gctgcggcgg ctggcgcatt tcacgctcga taccgtttcc ggtgaaaccg aatggcgtag
    62461 ctcgtcgatg tctacccgca cggcgcaaaa ttctgttacc gatttactgt ttgttggtct
    62521 tgtgcaactc aatgatgtgg agtcattgaa aatgattcag cgcagcagtg agctaacaca
    62581 acgcctgaaa tgatgcataa agcagcgact ggattgagat tttcctgaat tagtgagctg
    62641 atccgcagca atattttgtt tatcctgtat tttcagaggg aatggagtgt aacgctctgt
    62701 attaacaagg agagcattaa aatgggtaaa ctcacgggca agacagcact gattacgggc
    62761 gcattgcagg gaattggcga aggaattgcc agaacttttg cgcgtcatgg cgcaaaccta
    62821 atcttgctgg atatctcccc tgagatcgaa aagctggcgg acgaactgtg tggtcgtggt
    62881 catcgctgta cggcggttgt cgccgatgtg cgtgacccgg cgtcggtagc cgcagctatc
    62941 aaacgcgcga aggaaaaaga agggcgcatt gatatcctgg tgaataacgc aggcgtttgt
    63001 cgtctgggca gtttcctcga tatgagcgat gacgatcgcg atttccatat tgacatcaat
    63061 attaaaggcg tatggaacgt cacgaaggcg gtgctgccgg agatgattgc ccgcaaagat
    63121 ggtcgcattg tgatgatgtc ttcagtcact ggtgatatgg tggccgatcc tggcgaaacg
    63181 gcgtacgcct taacgaaagc ggcgattgtt ggcctgacaa aatcgctggc ggtggagtac
    63241 gcgcagtctg gtattcgcgt taacgccatt tgcccgggat acgtgcgcac accaatggcg
    63301 gaaagcattg cccgccagtc gaacccggaa gatccagagt cggtgctgac tgaaatggcg
    63361 aaagcaatcc cgctgcgtcg cctcgccgat ccgctggaag tcggcgaact ggcggccttc
    63421 ctcgcatcgg atgaatccag ctatttaacc ggtacacaga atgtgattga tggcggcagc
    63481 acactgccgg agacggttag cgtcggtatc tgattcacct ctgtttcctc cctgcatttg
    63541 tggggaggat ttcgttttaa acccggttct tattcccttt tcaacttcca aatcaccaaa
    63601 cggtatataa aaccgttact cctttcacgt ccgttataaa tatgatggct attagaaagt
    63661 cattaaattt ataagggtgc gcaatggccg ttaacttact gaaaaagaac tcactcgcgc
    63721 tggtcgcttc tctgctgctg gcgggccatg tacaggcaac ggaactgctg aacagttctt
    63781 atgacgtctc ccgcgagctg tttgccgccc tgaacccgcc gtttgagcaa caatggacca
    63841 aagaaaacgg cggcgacaaa ctgacaataa aacaatctca tgccgggtca tcaaaacagg
    63901 cgctggcaat tttgcagggc ttaaaagcag acgttgtcac ttataaccag gtgactgacg
    63961 tacaaatcct gcatgacaaa ggcaagctga tcccggcgga ctggcaaacg cgcctgccaa
    64021 ataatagctc gccattctac tccaccatgg gcttcctggt gcgtaagggc aacccgaaga
    64081 atatccacga ctggaacgac ctggtgcgct ccgatgtgaa gctgattttc ccgaacccga
    64141 aaacgtcggg taacgcgcgt tacacctatc tggcggcatg gggcgcagcg gataaagctg
    64201 acggtggcga taaagccaaa accgaacagt ttatgaccca gttcctgaaa aacgttgaag
    64261 tgttcgatac tggcggtcgt ggcgcgacca ccacttttgc cgagcgcggc ctgggcgatg
    64321 tgctgatcag cttcgagtcg gaagtgaaca acatccgtaa acagtatgaa gcgcagggtt
    64381 ttgaagtggt gattccgaaa accaacattc tggcggaatt cccggtggcg tgggttgata
    64441 aaaacgtgca ggccaacggt acggaaaaag ccgccaaagc ctaccttaac tggctctaca
    64501 gcccgcaggc gcaaaccatc atcaccgact attactaccg cgtgaataac ccggaagtca
    64561 tggataaact gaaagacaaa ttcccgcaga ccgagctgtt ccgcgtggaa gacaaatttg
    64621 gctcctggcc ggaagtgatg aaaacccact tcaccagcgg cggcgagtta gacaagctgt
    64681 tagcggcggg gcgtaattaa tgtttgcagt ttcatcaaga cgcgtgctgc cgggctttac
    64741 cttaagcctc ggcaccagtc tgctgtttgt gtgcctgatt ttgctgctgc cgctctccgc
    64801 gctggtgatg cagctggctc agatgagctg ggcgcagtac tgggaggtga tcaccaaccc
    64861 gcaggtagta gcggcctaca aagtaacgct gctgtcagcg tttgtggcat cgatttttaa
    64921 cggcgttttt ggcctgctga tggcgtggat cttaacccgc tatcgctttc cgggccgcac
    64981 gctgcttgat gcgctgatgg atttaccctt tgcgctgcca acggcagtcg ccggtttaac
    65041 gctagcctcg ctcttttctg taaacggttt ttacggtgaa tggctggcga agtttgatat
    65101 caaagtcacc tatacctggc tggggattgc ggtggcgatg gcctttacca gcattccgtt
    65161 tgtggtacgt accgtgcagc cggtgctgga agagttaggc ccggaatatg aagaagcggc
    65221 agaaacgctt ggcgcaacgc gctggcagag tttctgcaaa gtggtgctgc cggaactttc
    65281 tccggcgctg gtggcgggcg tggcgctgtc gtttacccgt agtcttggtg aatttggcgc
    65341 ggtgattttt atcgccggaa atatcgcgtg gaagacggaa gtgacgtcgc tgatgatttt
    65401 tgtgcgctta caggagtttg attacccggc agcgagcgcg attgcttcgg tgatcctcgc
    65461 ggcatctctg ctgctgctgt tctcaattaa cactctgcaa agtcgctttg gtcggcgtgt
    65521 ggtaggtcat taatggcgga agttacccaa ttgaagcgtt atgacgcgcg cccgattaac
    65581 tggggcaaat ggtttctgat tggcatcggg atgctggtat cggcgttcat cctgctggtg
    65641 ccgatgattt atatcttcgt acaggcgttc agcaaagggc tgatgccggt tttacagaat
    65701 ctggccgatc cggacatgct gcacgccatc tggctgacgg tgatgatcgc gctgattgcc
    65761 gtaccggtaa acctggtgtt cggcattctg ctggcctggc tggtgacgcg ctttaacttc
    65821 cctggacgcc agttactgct gacgctactg gacattccgt ttgccgtatc gccggtggtt
    65881 gccggtctgg tgtatctgct gttctacggc tctaacggcc cgctgggcgg ctggctggat
    65941 gaacataatc tgcaaattat gttctcctgg cccggaatgg tgctggtcac catctttgtg
    66001 acgtgtccgt ttgtggtgcg cgagctggtg ccggtgatgt taagccaggg cagccaggaa
    66061 gacgaagcgg cgattttgct tggcgcgtct ggctggcaga tgtttcgccg cgtcacattg
    66121 ccgaacattc gctgggcgct gctttatggc gtggtgttga ccaacgcccg cgcaattggc
    66181 gagtttggcg cggtgtcggt ggtttccggc tcgattcgcg gcgaaaccct gtcgctgccg
    66241 ttacagattg aattgctgga gcaggactac aacaccgtcg gctcctttac cgctgcggca
    66301 ctgttaacgc tgatggcgat tatcaccctg tttttaaaaa gtatgttgca gtggcgcctg
    66361 gagaatcagg aaaaacgcgc acagcaggag gaacatcatg agcattgaga ttgccaatat
    66421 taagaagtcg tttggtcgta cccaggtgct gaacgatatc tcactggata ttccttcagg
    66481 tcagatggtc gcgttgctgg ggccgtccgg ttccgggaaa accacgctgc tgcgcattat
    66541 cgccgggctg gagcatcaaa ccagcgggca tattcgcttc cacggcaccg acgtcagccg
    66601 cctgcacgcg cgcgatcgca aggtcggttt cgtgttccag cattacgcgc tgttccgcca
    66661 tatgacggta ttcgacaata tcgcttttgg cctgacggtg ctgccgcgtc gcgatcgccc
    66721 gaatgccgca gccatcaaag cgaaagtgac aaaattgctg gagatggtgc agcttgctca
    66781 tctggcggat cgttatccgg cacagctttc cggcggccag aaacagcgcg tggcgctggc
    66841 gcgcgcgctg gctgtagaac cgcaaattct gctgcttgat gaaccgtttg gcgcgctgga
    66901 tgcgcaggtg cgtaaagagc tgcgtcgctg gctgcgtcaa ctgcatgaag agctgaaatt
    66961 caccagcgtg ttcgtgaccc acgaccagga agaagcgacg gaagtagctg atcgtgtagt
    67021 ggtgatgagc cagggcaata ttgaacaggc tgatgcgccg gagcaggtat ggcgcgaacc
    67081 agcgacccgt tttgtgctcg aatttatggg cgaagtgaac cgcctgcagg gaaccattcg
    67141 cggcgggcag ttccatgttg gcgcacatcg ctggccgctg ggctacacac ctgcttatca
    67201 ggggccggtg gatctcttcc tgcgcccgtg ggaagtggat atcagccgcc gtaccagtct
    67261 cgattcgccg ctgccggtac aggtactgga agccagcccg aaaggtcact acacccaatt
    67321 agtggtgcag ccgctggggt ggtacaacga accgctgacg gtcgtgatgc atggcgacga
    67381 tgccccgcag cgtggcgagc gtttattcgt tggtctgcaa catgcgcggc tgtataacgg
    67441 cgacgagcgt atcgaaaccc gcgatgagga acttgctctc gcacaaagcg cctgataggt
    67501 tgagtgaatg ttaaacgccc ggaggcgctt cccgcgatcc gggcttttta atggcaaggt
    67561 ttgtaacctg tagacctgat aagacgcgca agcgtcgcat caggcaacac cacgtatgga
    67621 tagagatcgt gagtacatta gaacaaacaa taggcaatac gcctctggtg aagttgcagc
    67681 gaatggggcc ggataacggc agtgaagtgt ggttaaaact ggaaggcaat aacccggcag
    67741 gttcggtgaa agatcgtgcg gcactttcga tgatcgtcga ggcggaaaag cgcggggaaa
    67801 ttaaaccggg tgatgtctta atcgaagcca ccagtggtaa caccggcatt gcgctggcaa
    67861 tgattgccgc gctgaaaggc tatcgcatga aattgctgat gcccgacaac atgagccagg
    67921 aacgccgtgc ggcgatgcgt gcttatggtg cggaactgat tcttgtcacc aaagagcagg
    67981 gcatggaagg tgcgcgcgat ctggcgctgg agatggcgaa tcgtggcgaa ggaaagctgc
    68041 tcgatcagtt caataatccc gataaccctt acgcccatta caccactacc gggccggaaa
    68101 tctggcagca aaccggcggg cgcattaccc attttgtctc cagcatggga acaaccggca
    68161 ctatcaccgg cgtttcacgc tttatgcgcg aacaatccaa accagtaacg attgttggcc
    68221 tgcagccgga agaaggcagc agtattcccg gcattcgccg ctggcctgcg gaatatctgc
    68281 cggggatttt caacgcttct ctggtggatg aggtgctgga tattcatcag cgcgatgcag
    68341 aaaacaccat gcgcgaactg gcagtgcggg aaggaatatt ctgtggcgtc agctccggcg
    68401 gcgcggttgc cggagcactg cgggtggcaa aagctaaccc tggtgcggtg gtggtagcga
    68461 tcatctgcga tcgtggcgat cgctaccttt ccaccggggt gtttggggaa gagcatttta
    68521 gtcagggggc ggggatttaa gattattgcg caggcaccgt tttatccgtc tgcgcaatta
    68581 aggcatgtaa aaaaggctgt gcgttctcat cgctcttacc ggggatcttc acaatatacg
    68641 gcttcccggt aatcgatgac gacgaagcaa ccttatcaat aaactgctcg gcagtgtcaa
    68701 tgcggttacg ggtattgccg agcttcagac gcagatgaga aaccgcttca tcgcaggtat
    68761 gttcatcacc gttgcgcaca aatatcaaat cctttttctg tgctaatccc tccagcatgg
    68821 cgttgatgcg ggcttcttca tgaacagtta acttcgcgta agcggggagt gtcattagta
    68881 gtgtaatgac cagacaaatg attttcttca ttgaattacc ttatccttgt tttgtgatag
    68941 ccgatttgac tgccagaatc aggtaaagtt tcggttggaa tacgatgagg atcatcggca
    69001 aggagaaaaa attaaatgag tagtttgttg ttgtttaacg ataagagtag ggcgctgcag
    69061 gcggatatcg tcgccgtgca gtcgcaggta gtgtacggca gcgtaggcaa cagcattgcg
    69121 gtgcctgcta taaaacagaa cggcttgaat gtctttgccg tgccgacggt attgctgagc
    69181 aatacgccgc attatgacac tttctacggt ggtgcgattc cggacgaatg gtttagcggc
    69241 tatttgcgtg cgcttcagga gcgtgatgcg ctgcgccaac ttcgtgctgt aaccacgggc
    69301 tatatgggaa cggcatcgca aatcaaaatc cttgccgagt ggctgactgc gctacgcaaa
    69361 gaccatcctg acctattgat catggtcgat ccggtgattg gcgatattga tagcggaatt
    69421 tatgtcaaac ctgaccttcc cgaggcgtat cgacaatatt tactgccgct ggcgcaagga
    69481 attaccccca atatctttga gctggaaatc ctgaccggta aaaattgccg cgatctcgac
    69541 agtgccattg ctgccgcaaa aagtctgctt tcagacacat taaaatgggt ggtgattacc
    69601 agcgcctccg gtaatgaaga aaatcaggag atgcaggtgg tggtggtcag tgccgacagc
    69661 gtgaatgtca tttcccattc acgggtaaaa accgacctga aagggactgg cgacctgttt
    69721 tgtgctcagc ttatcagtgg tttgctgaaa gggaaggcgt taaacgatgc ggtgcaccgt
    69781 gcggggttgc gcgtactgga agtgatgcgc tatacccagc agcatgagag cgatgaattg
    69841 attttgccgc cgctggcgga agcataaaaa atggcgccga tgg
//
LOCUS       DAJTYZ010000004        62995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000004 DAJTYZ010000000
VERSION     DAJTYZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.004"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..405)
                     /locus_tag="OO596_000237"
                     /pseudo
     CDS             complement(1..405)
                     /locus_tag="OO596_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(511..3072)
                     /gene="mutS"
                     /locus_tag="OO596_000238"
     CDS             complement(511..3072)
                     /gene="mutS"
                     /locus_tag="OO596_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6716209.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3148..3282
                     /locus_tag="OO596_000239"
     CDS             3148..3282
                     /locus_tag="OO596_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716210.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3359..3712
                     /locus_tag="OO596_000240"
     CDS             3359..3712
                     /locus_tag="OO596_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6716211.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3749..5826)
                     /gene="flhA"
                     /locus_tag="OO596_000241"
                     /pseudo
     CDS             complement(3749..5826)
                     /gene="flhA"
                     /locus_tag="OO596_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5900..6910)
                     /gene="hypE"
                     /locus_tag="OO596_000242"
     CDS             complement(5900..6910)
                     /gene="hypE"
                     /locus_tag="OO596_000242"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6716212.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6907..8028)
                     /gene="hypD"
                     /locus_tag="OO596_000243"
     CDS             complement(6907..8028)
                     /gene="hypD"
                     /locus_tag="OO596_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6716213.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8028..8300)
                     /gene="hypC"
                     /locus_tag="OO596_000244"
     CDS             complement(8028..8300)
                     /gene="hypC"
                     /locus_tag="OO596_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6716214.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8291..9163)
                     /gene="hypB"
                     /locus_tag="OO596_000245"
     CDS             complement(8291..9163)
                     /gene="hypB"
                     /locus_tag="OO596_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6716215.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9167..9517)
                     /gene="hypA"
                     /locus_tag="OO596_000246"
     CDS             complement(9167..9517)
                     /gene="hypA"
                     /locus_tag="OO596_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6716216.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9729..10190
                     /gene="hycA"
                     /locus_tag="OO596_000247"
     CDS             9729..10190
                     /gene="hycA"
                     /locus_tag="OO596_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6716217.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10315..10926
                     /gene="hycB"
                     /locus_tag="OO596_000248"
     CDS             10315..10926
                     /gene="hycB"
                     /locus_tag="OO596_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6716218.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10923..12749
                     /gene="hycC"
                     /locus_tag="OO596_000249"
     CDS             10923..12749
                     /gene="hycC"
                     /locus_tag="OO596_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6716219.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12752..13675
                     /gene="hycD"
                     /locus_tag="OO596_000250"
     CDS             12752..13675
                     /gene="hycD"
                     /locus_tag="OO596_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6716220.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13693..15390
                     /gene="hycE"
                     /locus_tag="OO596_000251"
     CDS             13693..15390
                     /gene="hycE"
                     /locus_tag="OO596_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6716221.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            15400..15942
                     /gene="hycF"
                     /locus_tag="OO596_000252"
     CDS             15400..15942
                     /gene="hycF"
                     /locus_tag="OO596_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR6716222.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15942..16709
                     /gene="hycG"
                     /locus_tag="OO596_000253"
     CDS             15942..16709
                     /gene="hycG"
                     /locus_tag="OO596_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR6716223.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16706..17116
                     /gene="hycH"
                     /locus_tag="OO596_000254"
     CDS             16706..17116
                     /gene="hycH"
                     /locus_tag="OO596_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR6716224.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17109..17579
                     /gene="hycI"
                     /locus_tag="OO596_000255"
     CDS             17109..17579
                     /gene="hycI"
                     /locus_tag="OO596_000255"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR6716225.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17604..18377
                     /locus_tag="OO596_000256"
     CDS             17604..18377
                     /locus_tag="OO596_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716226.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWRFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            18539..18850
                     /locus_tag="OO596_000257"
     CDS             18539..18850
                     /locus_tag="OO596_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR6716227.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18847..19266
                     /locus_tag="OO596_000258"
     CDS             18847..19266
                     /locus_tag="OO596_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6716228.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(19380..20804)
                     /gene="ascB"
                     /locus_tag="OO596_000259"
     CDS             complement(19380..20804)
                     /gene="ascB"
                     /locus_tag="OO596_000259"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR6716229.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(20813..22270)
                     /gene="ascF"
                     /locus_tag="OO596_000260"
     CDS             complement(20813..22270)
                     /gene="ascF"
                     /locus_tag="OO596_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR6716230.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22530..23540
                     /gene="ascG"
                     /locus_tag="OO596_000261"
     CDS             22530..23540
                     /gene="ascG"
                     /locus_tag="OO596_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6716231.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIT
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            23688..24215
                     /gene="hydN"
                     /locus_tag="OO596_000262"
     CDS             23688..24215
                     /gene="hydN"
                     /locus_tag="OO596_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6716232.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            24368..26620
                     /gene="hypF"
                     /locus_tag="OO596_000263"
     CDS             24368..26620
                     /gene="hypF"
                     /locus_tag="OO596_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6716233.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(26748..27881)
                     /gene="norW"
                     /locus_tag="OO596_000264"
     CDS             complement(26748..27881)
                     /gene="norW"
                     /locus_tag="OO596_000264"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6716234.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(27878..29317)
                     /gene="norV"
                     /locus_tag="OO596_000265"
     CDS             complement(27878..29317)
                     /gene="norV"
                     /locus_tag="OO596_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6716235.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            29504..31018
                     /gene="norR"
                     /locus_tag="OO596_000266"
     CDS             29504..31018
                     /gene="norR"
                     /locus_tag="OO596_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6716236.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCKQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(31015..31980)
                     /gene="gutQ"
                     /locus_tag="OO596_000267"
     CDS             complement(31015..31980)
                     /gene="gutQ"
                     /locus_tag="OO596_000267"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6716237.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31973..32746)
                     /gene="srlR"
                     /locus_tag="OO596_000268"
                     /gene_synonym="gutR"
     CDS             complement(31973..32746)
                     /gene="srlR"
                     /locus_tag="OO596_000268"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6716238.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(32813..33172)
                     /gene="gutM"
                     /locus_tag="OO596_000269"
     CDS             complement(32813..33172)
                     /gene="gutM"
                     /locus_tag="OO596_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6716239.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(33278..34046)
                     /gene="srlD"
                     /locus_tag="OO596_000270"
                     /pseudo
     CDS             complement(33278..34046)
                     /gene="srlD"
                     /locus_tag="OO596_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            complement(34050..34421)
                     /gene="srlB"
                     /locus_tag="OO596_000271"
                     /gene_synonym="gutB"
     CDS             complement(34050..34421)
                     /gene="srlB"
                     /locus_tag="OO596_000271"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6716240.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(34432..35391)
                     /gene="srlE"
                     /locus_tag="OO596_000272"
                     /gene_synonym="gutE"
     CDS             complement(34432..35391)
                     /gene="srlE"
                     /locus_tag="OO596_000272"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6716241.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(35388..35951)
                     /gene="srlA"
                     /locus_tag="OO596_000273"
                     /gene_synonym="gutA"
     CDS             complement(35388..35951)
                     /gene="srlA"
                     /locus_tag="OO596_000273"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR6716242.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            36207..37292
                     /gene="mltB"
                     /locus_tag="OO596_000274"
     CDS             36207..37292
                     /gene="mltB"
                     /locus_tag="OO596_000274"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6716243.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37437..37934
                     /gene="pncC"
                     /locus_tag="OO596_000275"
     CDS             37437..37934
                     /gene="pncC"
                     /locus_tag="OO596_000275"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6716244.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            38014..39075
                     /gene="recA"
                     /locus_tag="OO596_000276"
     CDS             38014..39075
                     /gene="recA"
                     /locus_tag="OO596_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6716245.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            39303..39803
                     /gene="recX"
                     /locus_tag="OO596_000277"
     CDS             39303..39803
                     /gene="recX"
                     /locus_tag="OO596_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6716246.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            39931..42561
                     /gene="alaS"
                     /locus_tag="OO596_000278"
     CDS             39931..42561
                     /gene="alaS"
                     /locus_tag="OO596_000278"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6716247.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            42796..42981
                     /gene="csrA"
                     /locus_tag="OO596_000279"
     CDS             42796..42981
                     /gene="csrA"
                     /locus_tag="OO596_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6716248.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            43297..43389
                     /locus_tag="OO596_000280"
     tRNA            43297..43389
                     /locus_tag="OO596_000280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43331..43333,aa:Ser,seq:gct)
     gene            43393..43469
                     /locus_tag="OO596_000281"
     tRNA            43393..43469
                     /locus_tag="OO596_000281"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43427..43429,aa:Arg,seq:acg)
     gene            43533..43609
                     /locus_tag="OO596_000282"
     tRNA            43533..43609
                     /locus_tag="OO596_000282"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43567..43569,aa:Arg,seq:acg)
     gene            43808..43884
                     /locus_tag="OO596_000283"
     tRNA            43808..43884
                     /locus_tag="OO596_000283"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43842..43844,aa:Arg,seq:acg)
     gene            44165..44731
                     /gene="yqaB"
                     /locus_tag="OO596_000284"
     CDS             44165..44731
                     /gene="yqaB"
                     /locus_tag="OO596_000284"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6716249.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            44728..45156
                     /locus_tag="OO596_000285"
     CDS             44728..45156
                     /locus_tag="OO596_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6716250.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            45229..46785
                     /gene="gshA"
                     /locus_tag="OO596_000286"
     CDS             45229..46785
                     /gene="gshA"
                     /locus_tag="OO596_000286"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6716251.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEVYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            46935..47450
                     /gene="luxS"
                     /locus_tag="OO596_000287"
     CDS             46935..47450
                     /gene="luxS"
                     /locus_tag="OO596_000287"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6716252.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(47514..49052)
                     /gene="emrB"
                     /locus_tag="OO596_000288"
     CDS             complement(47514..49052)
                     /gene="emrB"
                     /locus_tag="OO596_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6716253.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(49069..50241)
                     /gene="emrA"
                     /locus_tag="OO596_000289"
     CDS             complement(49069..50241)
                     /gene="emrA"
                     /locus_tag="OO596_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR6716254.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(50368..50898)
                     /gene="emrR"
                     /locus_tag="OO596_000290"
     CDS             complement(50368..50898)
                     /gene="emrR"
                     /locus_tag="OO596_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6716255.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(50989..51324)
                     /gene="ygaH"
                     /locus_tag="OO596_000291"
     CDS             complement(50989..51324)
                     /gene="ygaH"
                     /locus_tag="OO596_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6716256.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(51314..52051)
                     /gene="ygaZ"
                     /locus_tag="OO596_000292"
     CDS             complement(51314..52051)
                     /gene="ygaZ"
                     /locus_tag="OO596_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6716257.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(52175..53359)
                     /locus_tag="OO596_000293"
     CDS             complement(52175..53359)
                     /locus_tag="OO596_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6716258.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(53650..54642)
                     /gene="proX"
                     /locus_tag="OO596_000294"
     CDS             complement(53650..54642)
                     /gene="proX"
                     /locus_tag="OO596_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6716259.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(54700..55764)
                     /gene="proW"
                     /locus_tag="OO596_000295"
     CDS             complement(54700..55764)
                     /gene="proW"
                     /locus_tag="OO596_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6716260.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(55757..56959)
                     /gene="proV"
                     /locus_tag="OO596_000296"
     CDS             complement(55757..56959)
                     /gene="proV"
                     /locus_tag="OO596_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6716261.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(57315..58274)
                     /gene="nrdF"
                     /locus_tag="OO596_000297"
     CDS             complement(57315..58274)
                     /gene="nrdF"
                     /locus_tag="OO596_000297"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6716262.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(58284..60428)
                     /gene="nrdE"
                     /locus_tag="OO596_000298"
     CDS             complement(58284..60428)
                     /gene="nrdE"
                     /locus_tag="OO596_000298"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6716263.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(60401..60811)
                     /gene="nrdI"
                     /locus_tag="OO596_000299"
                     /pseudo
     CDS             complement(60401..60811)
                     /gene="nrdI"
                     /locus_tag="OO596_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(60808..61053)
                     /gene="nrdH"
                     /locus_tag="OO596_000300"
     CDS             complement(60808..61053)
                     /gene="nrdH"
                     /locus_tag="OO596_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6716264.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(61301..61630)
                     /locus_tag="OO596_000301"
     CDS             complement(61301..61630)
                     /locus_tag="OO596_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6716265.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            61782..62126
                     /locus_tag="OO596_000302"
     CDS             61782..62126
                     /locus_tag="OO596_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6716266.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(62163..62612)
                     /gene="alaE"
                     /locus_tag="OO596_000303"
     CDS             complement(62163..62612)
                     /gene="alaE"
                     /locus_tag="OO596_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6716267.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT        14825 a        16243 c        16978 g        14949 t
ORIGIN      
        1 ttttatgttg tcattaatga tttcaataat atgtggaagg tgatggaatt tcagcaacag
       61 atctattgcc tcttgttgct ctgaatcatt taaatcgaaa aaccagtcac caccgctggc
      121 aagccacata tttccatcgc cagtttcgaa tgcatccaac gccatcgctt cgtggttgcc
      181 tttaaccgac gtaaaccagg gttggtttag caggcgcagc acgttaagac tcttcggccc
      241 acgatcaata ttatcgcctg tggaaataag taagtcggtt tcagggtaaa aagagagttg
      301 atgttaacgg gattgtaata actgatattc accatgaata tcaccaacga cccatatatg
      361 gcgatagtga tgggcattga ttttttgata gcgtgtagat ggcatggttt taccctgtaa
      421 aataagcttt cctattatac agggtatttt tatttgattc gtcagttgtc gttaatattc
      481 ccgatagcaa aagactatcg ggaattgtta ttacaccaga ctcttcaagc gataaatcca
      541 ctccagcgcc tgacgcggag tcagtgaatc cgggtcgaga ttttccagtg cttcgaccgc
      601 aggcgaagtt tcttctggca ctgacagcaa agacatctgc gtaccatcca cttgcgtcgc
      661 ggcggcgttc ggcgaaatgc tttccagctc acgcagtttt tgccgtgcgc gcttaataac
      721 ctctttcggt acgcccgcca gagcagcaac cgccagaccg tagcttttgc tcgccgcgcc
      781 gtcctgcacg ctgtgcataa aggcgatagt gtcgccgtgc tccagcgcat cgagatgtac
      841 gttggcgacg ccttccattt tctccggtag ctgggtcagc tcgaaatagt gggtggcaaa
      901 cagcgtcaat gccttaatct tattcgccag attttctgcg cacgcccacg ccagcgacag
      961 accatcgtag gtggacgttc cacgcccgat ctcatccatt aacaccagac tgtattcggt
     1021 ggcgttatgt aaaatattgg cggtttcagt catctccacc ataaaggttg agcgcccgga
     1081 agccaggtca tctgccgcgc ctacgcgggt aaagatgcga tcgataggtc caatctcgac
     1141 tttttgtgcc ggtacatagc tgccgatgta ggccatcagc gcaatcagtg cggtctggcg
     1201 catataggta cttttaccgc ccatgttcgg accggtgatg atcaacatac ggcgctgcgg
     1261 cgacagattc agcgggttag cgataaatgg ctcattcagt acttgttcaa ctaccggatg
     1321 gcgaccttcg gtaatgcgaa tgcccggttt atcaatgaag gtcgggcagg tgtagttcag
     1381 ggtataggcc cgttccgcca ggttcaccag cacgtcgagt tccgccagcg cgctcgcgct
     1441 ctgttgcaac gcttccagat gcggcaacag caggtcgaac aactcttcat aaagctgttt
     1501 ttccagtgcc agtgctttgc cttttgaggt gagaacttta tcttcgtact cttttagctc
     1561 tggaatgatg tagcgctcgg cgtttttcag cgtctggcga cgcatgtagt tgatgggtgc
     1621 cagatggctt tgcccacggc tgatttgaat gtagtagccg tgcaccgcat taaagccaac
     1681 tttcagcgtg tccaggccgg tacgttcacg ctcgcggact tccagacgct ccagataatc
     1741 ggtcgcgccg tcagccagcg cgcgccactc atccagctct tcgttatagc ccgatgcgat
     1801 aacaccaccg tcgcgtacca gcaccggcgg tgtgtcgatg attgctcgct ccagcagatc
     1861 gcgcagctcg gcaaactcgc ccatcttctc acgtagcgcc tgtaccggtg cactatcgac
     1921 agtttctaac tgcgcacgca gctccggcag ttgctggaaa gcgtggcgca tacgggccag
     1981 atcgcgtggg cgagcagttc gtaaagccag acgtgccaga atacgttcca ggtcgccgac
     2041 ctgacgcagt accggctgca actcggcggt gaaatcctgc aatgcgccaa tagtttgctg
     2101 gcgctcaagc aacacgcggg tatcgcgcac tggcatatgc agccagcgtt tcagcatacg
     2161 actacccatc ggcgtgacgg tgcagtcgag cacagaagcc agcgtatttt ccgcaccgcc
     2221 cgccaggttc tgagtaattt ccaggttacg acgtgtcgcg gcatccataa tgatgctgtc
     2281 ctgctcacgt tccatggtga tagaacgaat atgcggcagg gtcgtgcgtt gggtatcttt
     2341 cgcatactgc aacagacaac cggcagcaca aagtccgcgc ggcgcgttct cgacgccaaa
     2401 accgaccaga tcgcgggtgc caaattgcag attcaactgc tggcgcgcgg tgtcgatttc
     2461 aaactcccac agcgggcgac ggcgcaggcc gcgacggcct tcaatcagcg acatctcggc
     2521 gaaatcttct gcatacagca gttccgccgg attagtgcgt tgcagctctg ccgccatcgt
     2581 ttcgcggtcg gccggttcgc tcaggcgaaa acgcccggag ctgatatcca gcgtcgcgta
     2641 gccgaaacct ttgctgtcct gccagatagc cgccagcagg ttgtcctgac gctcctgtaa
     2701 cagggcttca tcgctgatgg tgcctggcgt aacgatacgc acaactttgc gctcaaccgg
     2761 acctttgctg gtcgccggat cgccaatttg ttcgcagatg gcaacggact ctccctgatt
     2821 caccagtttg gcgagatagt tttccaccgc atggtaggga atccccgcca tcgggatcgg
     2881 ctctcccgcc gaagcaccgc gtttggtcag tgaaatatcc agcagttgcg acgcgcgttt
     2941 tgcgtcgtca taaaacagtt cataaaaatc acccatccgg taaaacagca ggatctcggg
     3001 atgctgggct ttcagcctga gatactgctg catcatgggc gtatgggcgt cgaaattttc
     3061 tattgcactc atggggttat gtccggttcc ctgatattaa atggggtgtg atggttttta
     3121 ttttcgttgt aatcacataa ggcgcacatg ataccggagt taatcatttc aggggaagaa
     3181 gcgaacggcg agttcttgat gctggttccg ggaaagatgc tgttttgcag cttttgcgtg
     3241 atgcttatga agcaagtacg caaaattgcg tcggttgaat aaacctcctc cttcagagcc
     3301 atagtcccct ttcaaattaa gttgtaatat aaatacacct taagagtatg gaggcagcat
     3361 gtccggcaag cgtatctctc gtgaaaaact gacgattaaa aaaatgatcg atctttatca
     3421 agcgaaatgc ccgcaggcgt cagcggagcc ggagcattac gaggcgttgt ttgtttacgc
     3481 gcaaaagcgg ctggataaat gtgtgttcgg cgaagagaaa ccagcctgta agcagtgtcc
     3541 ggtgcactgt taccagcctg caaagcgtga agagatgaaa cagattatgc gttgggccgg
     3601 gccgagaatg ctgtggcgtc atccgatctt aaccgtgcgt catctcatcg atgacaagcg
     3661 tccggtacca gaactgccgg aaaaatatcg tccgaaaaaa cctcgtgaat agtatgccgg
     3721 acaggcagat ctgtccggca atttgcagtt aaatcaatgc cgatttatca attcccagcc
     3781 gcttcatccg tgacagcagg gtcgtgcgtt tcagccccag acgttgcgca gcgcctttag
     3841 gcccggcaac cacgccgttg gtttctttca gcacgcgcac aatcaactga tattcatctt
     3901 cgccctcctg ggcgacaacc gttgcggcag gcggcgtttc aggttccggt aaaacaatat
     3961 ctggcaatga cagctgcagc acgttaccgc gtgttagcaa taccgcgcgc tcaatgacgt
     4021 tttccagttc gcgtacgtta cccggccact ccatgttgct caaggtgcgc agcgtctcgg
     4081 caggaatgct gtcgatattg cgccccagac gacgggcaat tttgaaggta aaggctttcg
     4141 ccagcagcgg aatatcttcc ggacgctcgc gtagtggcgg caggtgaatc gggaatacgt
     4201 tcaggcggta atagagatcg ctacggaact ctcggtcggc gaccattttt ttcagatcgc
     4261 ggttagtcgc ggcaattaaa cgtacgtccg tctgaatgat tttgttgctg ccgagacgtt
     4321 caaactcctg ttcctgcaat acacgcagca acttcggctg taactccagt ggcatatcgc
     4381 ccacttcgtc gaggaacaga gagcttttat ccgccagttc aaaacgacca atgcgctggg
     4441 cgctggcacc ggtaaaagcc ccacgctcat gaccaaacag atcgctttcc agcaatccgg
     4501 caggcatcgc cgcgcagttc attttgacca tgcggcgatt attacgccca ctgagattat
     4561 ggatcgcgcg ggcaatcagc tctttacccg tgccagtttc accgaggatc agcacggtac
     4621 tgtcactttg cgccaccatt tcaacttgtt taagcacgct gtacatggct tcgctgcggc
     4681 caataatctc gccaaattca ctatcaacat tgttgagctg ctcggtcagg gcgaggtttt
     4741 catcaaccag ccgttctttc agacgatgga tttcctgata ggcgagggcg ttatcgacag
     4801 cgattgccac acgttcggca atctggcgca gcaaattcag attggtagtg gtaaacactt
     4861 tctcttcgca ttgcgccagt ttcagcacgc ccagcatggt gtcgccagac atcagcggta
     4921 acaggcacaa ggtttgaatc tggttgccct aggtgtcgaa caacatgcgt tcataggggg
     4981 ctaaatcgtc ccgctcgtgg agattgatca gcagcatctc tttacttttg aacacgcgtt
     5041 cggtgagggt tccggcttca tcgacttcgc tctgttcgtg ggcgggatgt tgtttatcaa
     5101 gatagtgagt ggagtagatg ttgagtttgt ttttacggtg gctgcgtaag acgatactga
     5161 tatcgtcaat gtcgaaatag taatggattt ctttggcgac ttcgctgacc agttcgtcca
     5221 tatccaggcg ggaaagcacc gcgttggtaa tggcaaccag gatgcggaag ttatcgcgct
     5281 cccggcataa caactcatag tcgacattgt tgacgacgcg gctctggatt tgttcggtga
     5341 cgacagaaac gatctgcgta aatgtttgca gacgattgaa ctctttttcg ctccagggac
     5401 gatcgtcata acgaataaat tcacagccac caaaaatatg cccttccgcc gccagtggca
     5461 tcaggcaata gtgaccaaat ttggggtata gcccacctgc gaccagctgc ggccaggttt
     5521 cacaaaattc ttcgtaactg caatgcagcg tatcagggcg cgacaaaatg cggcgtaccg
     5581 gaccgtgtgc cagaacagtt tcgtcttcat atttaatggg gtgtcttttt cacgcgatgc
     5641 gtaataagac gcacgttgag tctgcgcttg ccacaacaca atagccgcgt tgtcggcgag
     5701 cgcagaacgc tttaccagtt gcgaaagagc ctcacacagc gaggccagat cgggctgctg
     5761 caatagtgtc cgagtgatgt cgaacaaccc ttgctgtccg agatcactca tcggtgtata
     5821 tgacattttg ttcttccagg aaagcaccgc aaagcggtgc aaaaggtcat tgactaacta
     5881 taggagattt agaattttat tagcatatac gcggaagagg ttcggcgtgt ggtaaatcga
     5941 gggttcgttt cacaccatac agaccggcaa gacgaacacc tttacgttcc accacttcac
     6001 caattagcgc cgcgtctttc cccagtggat gggaatgtaa cgctgccagc acttgctctg
     6061 ccgcgttgcg ttcaacggcg atcaccagtt tgccttcgtt ggcaaagtta agggcgtcca
     6121 gtcccagcag ttcgcaaacg ccgcgcacgg caggtttaac cggcagtgcc acttctgaaa
     6181 tttcaatacc acaaccgcag gctgccgcga actcatgaac taccgcgttc acaccaccac
     6241 gggtggcatc acgcagcgct ttcacgccgg gaatgtcacg cagcgtctga ataagcggcg
     6301 tcagtaccgc gcagtcgctg accagttcgc catccagccc cagctgctca cgcaggttaa
     6361 ggatagtcgc cccgtggtcc ccgagcgtgc cgctaaccag taatacatcg cctgcggtta
     6421 gcgtctgtgc gccccagtga atattcgccg gaattgcgcc catgccagcg gtgttgataa
     6481 acagtttatc tgccgcgccg cgctgcacca ctttagtatc gccagtaacg atggcaatgc
     6541 ctgccgcgcg cgcggtttct gccatgctgg tcactacggc tttcagtgtc tccatcggca
     6601 atccttcttc gaggataaag ccacaggaga gatagcgcgg aatagcgcca ctgaccgcaa
     6661 cgtcattggc tgtgccgcaa atcgccagct tgccgatatt accgccagga aagaacagcg
     6721 ggtcaatgac gtaactgtcg gtggagaacg ccagacggtc gccttctgct accagctgcg
     6781 ccagatcaag acgtgcctgg tcttcctgct ctgccagcca cgggttggca aaggcttcca
     6841 taaacaggct gttgattaat tgctgcatcg cctggccgcc gctgccgtgg gcgagttgga
     6901 tattattcac gcttcattct cctgctgacg atactgatac cacgcggcgc acgctccttc
     6961 ggaggagacc atcagtgcac caaatgcggt ttgaggatta caggtgttac caaacagcgg
     7021 gcattgatgc ggcttacatt tgcccgttaa tacctcacca caacgcgcgc gcgggtcatc
     7081 gcagacctgc tgcggtgctg ggcggaaatg tgcttcggca tcgaatcgtt gataatccgg
     7141 cgtcaggtgc acgccagaag attcaatcac gcctaagccg cgccattcgc tgtcgccgtt
     7201 gacacagaac acatcagcaa ttgcctgttg cgccagcagg ttaccggcat ccggtaccac
     7261 ccgacgatac tgattctcta ccttgctgtg ggccgctatt ttctgctcca ccagcatgac
     7321 cacgccttgc agtagatcaa ggggttcgaa accagcgacc accagcggac gatgaaaatc
     7381 gctggcgata aaattatagg catcagtgcc gataaccata ctaacgtggc ccggcgcgag
     7441 gaacgcgtcg ataccgttat ccggctgttc cagcaaactg cgcagcgtcg ggataagcgt
     7501 aatatgctgg cagaagaagt aaaaattctg cacatcgcgg gctttcgcct gttgcagggt
     7561 gatggcggtg gtcggcatgg tggtttcaaa acctaagccg aagaatacga ctttacgggt
     7621 tggattctcc tgcgccagtt tcaacgcatc catcggcgag taaacgatgc gcacatcggc
     7681 accgcgtgct tttgcctgca acagcgatcc ctgtttcccc ggcacgcgca tggcgtcgcc
     7741 aaaggtacag aagatgactt ccggatggct ggcaatctcc acgcaggtgt cgattctacc
     7801 catcggcagt acgcacaccg ggcaacccgg accgtggata aactcaacgt tttcaggcag
     7861 taactggtcg aggccgaatt taaaaatggc gtgggtatga ccaccgcaca cttccataat
     7921 ccgcagaggg cgttcggcgg tgtaagagag atgtgaagca cgttcgcgca gatgctcaat
     7981 taactgcatc acctgttccg gcgcgcgata ttcatcaaca aaacgcatta tttttcctcg
     8041 ccatacaaca gcgcgccgac atccggctca acgtcaaaca tgttttgtaa ggcgtcgaga
     8101 gtgtcgcgtg cttcggcttc attaattacg ctcatggcaa agccaacgtg taccagtacc
     8161 cactggccca cgcgcggctg accgttttca tcgcagctgc cgactaacgt taaatcgaca
     8221 tcgcgctgaa tgccgcagac gtcgactttt gcctggttgc cgtcaatggt gcggatctgg
     8281 ccgggaacgc ctatgcacat cgctgtgtct ccagccagtt cagccactgg tccatccctt
     8341 cgccgctggt ggcggaaata aggatgattt caatttctgg attgacttcg cgggcgcagg
     8401 cgatgcactt ctcaacgtca aagttgagat acggcaacag gtcaactttg ttgagcagca
     8461 tcagcgaggc ggcggcaaac atatgcggat atttcagcgg tttgtcttca ccttcggtaa
     8521 cggaaagcac cgccactttg tgtttttcac cgagatcgaa gctggccggg catacgaggt
     8581 tgccaacgtt ttcgataaac agaataccgt tatcgtccag tggcagacgc ggtgcggcgt
     8641 cggcaatcat ctgtgcgtca agatggcagc ctttaccggt gttcacctga atcgctggtg
     8701 tgccggtagc gcgaatgcgt gcggcatcgt tcacggtttg ctggtcacct tcaataaccg
     8761 cgcacggaac gctgtctttc aggcgcatta gggtttccgt cagcagggtg gttttaccgg
     8821 aaccagggct ggaaaccagg ttgagcacca gttgcttgcg ggcagcaaag cgcgcgcggt
     8881 tgcgttcagc cagacggtta tttttgtcca gcacgtcaat ttcgacttcc agcatccgac
     8941 gctggctcat gcccggcgcg tgagtgcccg cttcgccatg accgtaatgc aggtcgcctt
     9001 cttcagtctg gctgggggta aattcaggcg ctttaatgcc ggtgattttc atcttcgggc
     9061 gtgccgccgg ggcaaatggc gcgctacgaa acgcggaatg agggttatgt tcatcaccct
     9121 cgatatacag gttgccttca ccgcaaccgc atgttgtaca catcgctcac tcctggtcta
     9181 tttctatccg ccgaatctgt aaaccgtcgt ctgccacaat ctgtagcatg tcaccatgac
     9241 actgtggaca gcggcggacg cgctgggtca gtagcgtcac atactgttgg catgtttcac
     9301 accagcattc ggcctcttgt tcttcgaggt gcagtttaca accttccgcc acgctgccgc
     9361 ggcaaaccag atcaaaacaa aaggcaagag agctggtttc gacacaagaa aatgcgccaa
     9421 ttttgagcca gaccccagtt acgcgttttg cgccgtgttt tgcggcctgc tgttcgatca
     9481 attccagtgc ccgttggcag agggttattt cgtgcatatc gcctcccatt aactattgcc
     9541 agctacaagc aataattgtg ccagtgttga ttatccctgc ggtgaataat gtcgatgatg
     9601 tcgaaatgac acgtcgacac ggcgacgaaa ttcatcttta gcttaaaaat ctatttaata
     9661 acaataaatt aaaagttggc atagaaaatg cttaaagctg gcatctctgt taaacgggta
     9721 acctgacaat gactatttgg gaaataagcg agaaagccga ttacatcgca cagcggcatt
     9781 gtcgcctaca ggaccagtgg cacatctact gcaattcgct ggttcagggg atcacgttat
     9841 cgaaagcgcg cctgcatcac gccatgagct gcgcgccgga caaagaactc tgtttcgtcc
     9901 tttttgaaca ttttcgtatt tacgtcaccc tggcggatgg ctttaacagc cacaccatcg
     9961 agtattacgt cgaaacaaaa gatggcgaag acaaacagcg gattgcgcag gcgcaactga
    10021 gcattgacgg catgattgat ggcaaggtca acatccgcga tcgcgaacag gttctggaac
    10081 actatctcga aaaaatcgct ggcgtttacg acagcttata caccgctatt gaaaacaatg
    10141 tgccggtgaa tttaagccaa ctggtaaagg gacaaagccc ggcagcatga gctgaggctt
    10201 tgcccgtttt gcaggcgtta cgcctgtttg gggatgggcg tgtcgatgag tgtcgaaaat
    10261 gacatttcat cggcatgttt tcgtcaaaaa tgacaatcac ctgaggaatg cctggtgaat
    10321 cgttttgtaa ttgttgactc cacgctctgt atcggctgcc acacttgtga ggccgcctgt
    10381 tcagagacgc atcgccagca cggcctgcaa tcaatgccgc gcctgagagt gatgctgaat
    10441 gaaaaagaat ctgcgccgca gctctgtcac cactgtgaag atgcaccctg tgcggtggtt
    10501 tgcccggtta acgccatcac ccgcattgat ggggccgtgc agttgaatga aagtctgtgc
    10561 gtaagctgca aactgtgcgg catcgcctgc ccgtttggcg caattgaatt ttccggcagc
    10621 cgtccgctgg atattccggc aaacgccaat accccgaaag cgccaccggc accgcctgct
    10681 ccggcgcgta tcagtacgct gcttgactgg gtgccgggta ttcgcgcgat cgccgtcaaa
    10741 tgtgaccttt gcagttttga tgaacaaggt ccggcctgcg tgcggatgtg cccgactaaa
    10801 gccctgcatc tggtggataa caccgatatc gcccgcgtca gcaaacgtaa gcgtgagctg
    10861 acctttaaca cggactttgg cgatctcacc ttgtttcagc aggctcaaag tggagaggct
    10921 aaatgagcgc aatttccctg atcaatagcg gcgtggcatg gtttgtcgcc gccgctgttc
    10981 tggcatttct cttttctttt caaaaagcgt taagtggctg gatagctgga attggcggcg
    11041 cggttggtag tctgtatacg gcagccgcgg gcttcactgt actgactggc gcggttggcg
    11101 tgagcggtgc gctgtcgctg gtaagctacg atgtgcaaat ctctccgctt aacgcgattt
    11161 ggctgattac gctcggtctg tgcggtctgt ttgtcagcct ctacaacatt gactggcatc
    11221 gccacgcgca ggtgaagtgc aacggcttgc agatcaatat gttgatggct gccgccgtct
    11281 gcgccgtcat cgccagcaac ctcggcatgt tcgtggtaat ggccgaagtc atggccttgt
    11341 gtgcggtgtt cctcaccagc aacagcaaag agggcaaact gtggtttgcg ctggggcgtc
    11401 ttggcactct gctgctggtg attgcttgct ggctgctgtg gcagcgttat ggcacgctgg
    11461 atctgtgcct actggatatg cgtatgcaac agctgccgct cggttccgat atctggctgc
    11521 tcggtgtgat tggctttggc ctgctggccg ggattattcc gctgcacggc tgggtgccgc
    11581 aggcacatgc taacgcctct gcgccagctg ccgcgttgtt ttctacggta gtcatgaaaa
    11641 ttggcctgct gggcatgtta accctgtcac tgctgggcgg taatgcaccg ctgtggtggg
    11701 ggatcgcgct gctggtgctc ggcatgatca ccgcgttcgt cggtggtctg tatgcgttga
    11761 tggaacacaa catccagcgc ctgctggctt accacaccct ggaaaatatc ggcattatcc
    11821 tgctggggct gggcgctggc gtaacgggta tcgcgctcga acaaccggcg ctgattgctc
    11881 ttggcctggt cggtggtctg taccatctgc ttaaccatag cctgttcaaa agcgtactgt
    11941 tcctcggggc ggggagcgtc tggttccgta ccggtcatcg cgatatcgaa aaactcggtg
    12001 gtattggcaa gaaaatgccg gttatctcca tcgccatgtt agtcgggttg atggcaatgg
    12061 ctgcgctgcc gccactgaac gggtttgccg gggaatgggt tatctatcaa tccttcttca
    12121 aactgagcaa tagcggcgcg tttgttgccc gtctgctggg gccgctgctc gctgtgggtc
    12181 tggcaattac cggtgcgctg gcggtgatgt gtatggcgaa agtctatggc gtcacgttcc
    12241 tcggcgcgcc gcgtaccaaa gaagccgaaa acgccacctg tgcgccgctc ctgatgagcg
    12301 taagcgtagt ggcactggcg atttgctgcg taattggcgg tgttgctgcg ccgtggctgc
    12361 tgccgatgct ctctgctgct gtacctctgc ctctggagcc tgctaacacc accgtttctc
    12421 aaccaatgat cacattgctg ctgattgcct gcccgctgct gccattcatc attatggcga
    12481 tttgcaaagg cgaccgcctg ccgtcacgtt cccgcggtgc ggcctgggtg tgcggctacg
    12541 accatgaaaa atcaatggtg attaccgctc acggttttgc catgccggtt aaacaggcgt
    12601 ttgcgccagt gctgaaactg cgcaaatggc tgaatccggt gtcgctggtg ccgggctggc
    12661 agtgcgaagg tagtgcgttg ctgttccgcc ggatggcgct ggttgaactg gcggtactgg
    12721 tggtgattat tgtttcacga ggagcctgag aatgagtgtt ttatatccgt taattcaggc
    12781 gctggtgtta tttgccgttg cgccgctgct ctccggtatt acccgcgtgg cgcgcgcccg
    12841 tctgcataac cgtcgtgggc cgggtgtgtt gcaggagtat cgtgacattt tcaaactgct
    12901 gggccgccag agcgttggcc cggatgcctc cggctgggtg ttccgcctga cgccgtatgt
    12961 gatggtgggc gtcatgctga ctatcgctac tgcgctgccg gtggtgaccg tcggttctcc
    13021 gctgccgcaa ctgggtgatt taatcacctt actgtatctc tttgccatcg cgcgtttctt
    13081 ctttgccatt tctggtctgg ataccggtag cccgtttacc gctatcggcg cgagccgtga
    13141 agcgatgctt ggcgtgctgg tcgaaccgat gctgctgctt ggtctgtggg ttgccgcaca
    13201 ggttgccggt tccaccaaca tcagcaacat caccgacacc gtttatcact ggccgctgag
    13261 ccagagcatc ccgctggtac tggcgctttg tgcctgtgcg ttcgccacct ttatcgaaat
    13321 gggcaaactg ccgttcgacc tggcggaagc cgagcaggag ctgcaggaag gcccgctctc
    13381 tgaatacagc ggcagcggct ttggcgtcat gaaatggggt atcagcctga aacagctggt
    13441 ggtgctgcag atgttcgtcg gcgtatttat cccgtgggga cagatggaaa ccttcaccgc
    13501 cggtgggctg ctgctggcgc tggtgattgc catcgtcaaa ctggtcgtcg gcgtcctggt
    13561 tatcgcgctg ttcgaaaaca gcatggcccg tctgcgtctt gatattactc cgcgcattac
    13621 ctggactggg tttggctttg catttttagc gttcgtctcc ttgctggcgg cgtgattaaa
    13681 gagagtttga gcatgtctga agaaaaatta ggtcaacatt atctcgccgc gctgaatgag
    13741 gcatttccgg gcgtcgtgct ggaccacgcc tggcagacca aagatcagct gactgtcacc
    13801 gtaaaggtga actatctgcc ggaagtggtg gagtttctct actacaagca ggggggctgg
    13861 ctgtcggtgc tttttggtaa cgacgaacgc aaactgaatg gtcattacgc cgtttattac
    13921 gtgctgtcga tggagaaggg gactaagtgc tgggtaacgg ttcgcgtcga agttgatgcc
    13981 aacaaaccgg agtatccgtc cgtgacgccg cgcgttccgg cggcggtgtg gggcgagcgc
    14041 gaagtgcgcg atatgtacgg tttgattccg gttggtctgc cggatgaacg ccgtctggtg
    14101 ctgccggatg aactttatcc gctgcgtaaa gacagcatgg attatcgtca gcgtccggca
    14161 ccgaccaccg atgctgaaac ctacgagttc atcaacgaac tgggcgacaa gaaaaacaac
    14221 gtcgtgccga ttggtccgct gcacgtcact tctgacgaac cgggccactt ccgtctgttc
    14281 gtcgatggcg aaaacattat cgacgccgac taccgtctgt tctacgttca tcgcggcatg
    14341 gaaaaactgg cggaaacccg tatgggttac aacgaagtga ccttcctctc tgaccgtgtg
    14401 tgcgggatct gcggctttgc ccacagcacc gcctacacca cgtcggtaga aaacgcgatg
    14461 ggtattcagg tgccagaacg tgcgcagatg atccgcgcca ttctgctgga ggtggaacgc
    14521 ctgcactcgc atctgctcaa cctcggtctc gcctgccact ttaccggctt tgactccggc
    14581 tttatgcagt tcttccgcgt gcgtgaaacc tccatgaaaa tggcagagat cctcaccggt
    14641 gcgcgtaaaa cctacggcct gaacctgatc ggcgggattc gtcgcgatct gttgaaagat
    14701 gacatgatcc agacccgcca gctggcgcaa cagatgcgtc gtgaagtgca ggagctggtg
    14761 gatgtgctgc tgagtacgcc gaacatggaa cagcgcaccg tcggcattgg tcgtctggac
    14821 ccggaaatgg cccgcgattt cagtaacgtt ggcccgatgg ttcgcgccag cggtcacgcc
    14881 cgcgatactc gcgccgatca cccgtttgtc ggttatggcc tgctgccaat ggaagtccac
    14941 agcgagcagg gctgcgacgt tatttcccgt ctgaaagtgc gtatcaacga agtttatacc
    15001 gcgctgaaca tgatcgacta cggtctggat aacctgccgg gcggtccgct gatggtggaa
    15061 ggctttacct acattccgca ccgtttcgcg ctgggctttg ccgaagcgcc gcgcggcgac
    15121 gatatccact ggagcatgac cggcgacaac cagaagctgt accgctggcg ctgccgtgcc
    15181 gcgacctacg cgaactggcc gaccctgcgc tacatgctgc gcggtaacac cgtttccgat
    15241 gcgccgctga ttatcggtag cttggaccct tgctactcct gtaccgaccg catgaccgtg
    15301 gtcgatgtgc gtaagaaaaa gagcaaagtg gtgccgtaca aagaactcga gcgttacagc
    15361 attgagcgta aaaactcgcc gctgaaataa ggaatcgcca tgtttacctt tatcaaaaaa
    15421 gtcatcaaaa ccggcacggc gacctcgtct tatccgctgg agccgattgc ggttgataaa
    15481 aacttccgtg gtaagccgga gcagaacccg cagcagtgca tcggctgtgc ggcctgcgtc
    15541 aatgcctgcc cgtcaaacgc cttaacggtt gaaactgacc tcgccaccgg tgagctggcg
    15601 tgggagttta atctcgggcg ctgcatcttc tgtggacgct gcgaagaagt ttgcccgacg
    15661 gcggcgatca aactgtcgca agagtacgaa ctggcagtgt ggaagaaaga agacttcctg
    15721 caacagtccc gcttcgcgct gtgcaactgc cgcgtctgca atcgtccttt cgccgtccag
    15781 aaagagatcg actacgccat tgcgctgctt aagcacaacg gcgacagccg cgcggaaaac
    15841 caccgcgaaa gctttgagac ttgcccggaa tgtaagcgcc agaaatgcct ggtgccgtcc
    15901 gaccgtattg aactgactcg ccatatgaaa gaggccatct gatgagcaat ttattaggcc
    15961 cccgtgacgc caacggcatt ccggtcccga tgacggtgga tgaatccatc gccagcatga
    16021 aggcgtcgtt actgaaaaaa atcaaacgtt ctgcctacgt ttatcgcgtg gactgcggcg
    16081 gctgcaacgg ctgcgaaatc gaaattttcg ccacgctttc gccgctgttt gatgcagaac
    16141 gcttcggcat taaagtcgtt ccttcaccgc gtcatgcgga tattttactg tttaccggcg
    16201 cggtcacccg tgcaatgcga tcccctgcgc tgcgtgcgtg gcagtccgcg ccggacccga
    16261 aaatttgtat ctcctacggt gcctgcggta acagcggtgg tattttccac gatctctact
    16321 gcgtgtgggg cggtacggat aaaatcgtcc cggtagatgt ctacattcct ggttgcccgc
    16381 caacgcctgc cgccacgctg tacggctttg caatggcgct cggcctgctg gagcagaaaa
    16441 ttcacgcccg tggcccgggt gagcaggatg aacaacaggc ggaaatcctg catggcgata
    16501 tggtgcagcc gctgcgtgtg aaagtggatc gcgaagcacg ccgcctggcg ggttatcgtt
    16561 acggtcgtca gattgccgat gacttcctga cacagttagg gcagggcgaa gaacaggttg
    16621 cacgctggct ggaagcggaa aacgatccgc gtctgaacga gattgtcagc catctgaatc
    16681 atgttgttga agaggcgcgt atccgatgag tgaaaaggtg gtgttcagtc aactgagccg
    16741 taaatttatt gatgagaacg atgccacgcc cgccgaggcg cagcaggtgg tctattacag
    16801 cctggcgatt ggtcaccacc ttggggttat cgattgcctg gaagcggcgc tcacctgccc
    16861 gtgggatgaa tatctggcat ggattgccac tctggaggcg ggcagtgaag cccgccgcaa
    16921 aatggaaggc gtgccgaaat atggtgagat cgtcatcgac attaaccatg tgccgatgct
    16981 ggccaacgca ttcgataaag cccgggcagc gcaaacttcg cagcagaagg aatggagtac
    17041 aatgctgtta agtatgctgc atgatattca tcaggaaaac gccatctatt tgatggtgag
    17101 gagactgcgt gactgacgtt ttactctgtg ttggcaatag catgatgggc gatgatggcg
    17161 ctggcccgct gctggcggaa aagtgcgccg ccgcgccgaa aggtaactgg gtggtgattg
    17221 atggcggtag tgcaccggaa aacgacatcg tcgccatccg tgaactgcgc cctacgcggt
    17281 tactgattgt tgacgctact gatatggggc taaaccccgg cgaaatccgc atcatcgacc
    17341 cggatgatat cgccgagatg tttatgatga ctacccataa catgccgctg aactatctta
    17401 tcgaccagtt gaaagaagac attggcgaag tgattttcct cggcatccag ccggatatcg
    17461 tcggctttta ctacccgatg actcagccga ttaaagatgc ggtagaaacc gtttatcaac
    17521 gactggaagg ctgggaaggg aatggcggct tcgcgcagtt agcggtggaa gaagaataat
    17581 tctccatcag ggaattcagc gaaatgaata tcagaacagg gatgtgcgct tttttattga
    17641 gcttagtgct gcctgcacag gccaccagct ttaccgaata tctgccaatg agtgacagcg
    17701 aatacgcgca aaagcgggcg ctgaaacctc tcctgaccat gccttatgat gccgagcaaa
    17761 cctggcgttt tagaaaagtg ggcgttgctg gcgtgacgct tgaaaagatg ccaaatgatg
    17821 atagtgaatg gcaattgaac ggtaaagatc gcgccggtaa atcctggtct gtgcctgtgg
    17881 gtatgctggc aaacatagcg ggcaaagggc aactctatcg cgccgatctt gatcgtaacg
    17941 gcattcagga tctggttatc tggttaccaa gtaccggtaa tggtctggct ccttatgcac
    18001 atttaattct tatgacgttc acgcgcgaag gccgtccctg tgtctttgaa cccaggggat
    18061 tctacaccgc aagcaaaact ggcgtagatg atttgctgga tttgcaaggc aatggtcaca
    18121 cgcaattgct ggatatgcag tttgattccg gttactggat aaccagtctg tatcaggtaa
    18181 aagacgccaa atggcagcgt gttcacggct ggtttggcaa gttaagctat cccgcgctca
    18241 cgcgttttac ttataccccc aaccgtaaac tcgtcctcaa acccatcgcc gggcgcgacc
    18301 cgcaaacgga agatctggcg cagacgcagc gttgcttgat aaagggggat gttcttgatg
    18361 gggtgaacca gaactgactt tttgcagagt ggctatgagg attttggggg taattatatc
    18421 gcgctcaagt gtttacatat tgtgaacttg gatttgatga gctatagttt ccgaacacat
    18481 aaggatagaa ctcgcagatg tggctgttta cttactataa ggaagttaaa acttaaaaat
    18541 gcacatcatt tctaaagcgc catttgaaga gtctgcgcgt aaatatccta acgacgctct
    18601 ggctcttcag gcattgtatc gggttattaa ggaaacggat ttttctacgc cagaagagat
    18661 gcgaacggcg tttcccaacc tcgacaattt taaatatcgc aacaaatggt acgttctgga
    18721 tgtaggtggg aacaacctaa gggtgattgc ctacataaac tttgttaata agcggttctt
    18781 tgtgaagcac ataactaatc acgccgagta tgacaaactg acacgttact acagagagaa
    18841 caaagaatga ctgctaatgc tgcacgcgct gttaaagcaa caagagaact ggtcaatgca
    18901 gtcccctttc tgggaggtag tgactcagaa gatgattatc gtgaagcact ggaactggtc
    18961 gaatatttga ttgaagaaga tgacaccaat ccacttatcg actttctggc gagccgcatt
    19021 gcagaatatg aaaacaacaa ccaagagttt gcagagttcg acaaagccgt tgcggcaatg
    19081 cctgttggcg tggcgttact tcgcacgctg attgatcaac ataatctgac ctatgcggat
    19141 ttgaagaacg aaatcggctc taaatccctg gtcagtcaga ttttgtctgg acaacgatcc
    19201 ctgacgatat cccacattaa ggcactttca gcccgatttg gcgttaagcc ggaatggttt
    19261 ctttgatttt gactgatgcc aaaccccaga ttgcccgatg cgctgcgctt atcgggcctt
    19321 cctggttcgt gcgacatgta ggccggataa ggcgttcacg ccgcatccgg cactgttacc
    19381 tactctaaat cttccccatt actggcaatc acttttttat accaccagaa cgatttctta
    19441 cgtgtgcgcg tcagcgtgcc gttgcctgcg tcgtcacggt ctacgtagac aaaaccgtag
    19501 cgtttgctca tttcacccgt agaggcggag actaaatcaa tacagcccca ggtggtgtag
    19561 cccatcagcg gaatgccatc tgcaatcgct tcgcccattg cgcggatatg ttcgcgtaaa
    19621 tagctgatgc gatagtcatc gttaatctcg ccattggcag caagttcatc ttttgcgccg
    19681 aggccgtttt ccaccagaaa cagcggcttc tgataacggt cgtacatcat gttcatggtg
    19741 atacgcagac cgagtggatc aataccccag ccccagtcgc tcacttgcag atacgggtta
    19801 cgcagcgatt tcactacgtt cgccgcactg ctattgttgg cgttcatctc cgccgaggcg
    19861 cagcgcgagg cgtaatagct gaaagagaca aaatcgacgg tgtttttcag gatttcatca
    19921 tcgcccggtg ctttgtcgat ggttacccct ttttcgcgga atacgcgggc agagtaagcc
    19981 ggatacgtgc cccgcgcctg cacatcgata aaaaacaggt tttcccggtc tttctccagc
    20041 gccgcccaga catcttccgg cttgcaactg taagggtaga agttaccgcc cgccagcata
    20101 cagcccacct gattttgcgg gttaacctca tgggcgattt tggtggctag cgcactggca
    20161 accagctggt gatgcgcggc ctgatatttc acctgatcct gattttcacc ttcttcaaac
    20221 accagacccg cgccggagaa cgggctatgc aacataatgt tgatttcatt gaaggtaagc
    20281 cagtatttca ccagaccatc aaatgcttca aagcaggtcc gggcgtagcg gctgaaaaac
    20341 tccaccagct tgcggttacg ccaggagcca tattcggtga ccagatgcat cggcacatcg
    20401 aagtggcaca acgtgaccag cggttcgata ccgtactttt tacactcttc aaagacagaa
    20461 cgataaaaag caatgccctg ctgattgggc gtgatttcat cgccctgcgg aaagagacgg
    20521 ctccaggcaa ttgaggtacg gaaaaccttg aatcccatct ctgccatcag ggcgatatct
    20581 tctttataac gatgataaaa atccgtcgcc tcatggctgg gataaaactc gtcatcgcgc
    20641 aactgaaaac gtttttccag ccccagtttc accgccattc gatgctcgcc gtgtgggatc
    20701 atatcgacag tggtcagccc tttgccacct tcacggaacg caccttcaga ctggttggcg
    20761 gcaagcgcgc cgccccataa aaaaccttct ggaaatactg acattttcat cctcaattaa
    20821 gacttacttc tttggcgacg gtcggttgta cgctctgatg ctttcgcgcc tcggcagccg
    20881 cttcctcaac aggaatatcc tcaaagccga gcaacagtgt gaggataaac gacagcacca
    20941 ccgccagcgc catgaccgcg aacacccaga cgatgctcat tggattcgcc ggatcgaaga
    21001 attgcacgct ggtaaataac cctggcgctg ccattgagtg gctggcaagc cccgccatac
    21061 cggcaaccgc gccgcaaata aaaccgctga taagactggc gataagcgga cgtttcaggc
    21121 ggatcgccac gccgtataac gccggttcgg aaatccccgc cataatggct gatgccgccg
    21181 cagccagcgc cgtctggcgc agttccgggt ttttcgtttt ccacgccacc gccagtgatg
    21241 aaccgcccag cgacaggtta gcgccgatct ctgacggcat gaccatccct tctttgccgg
    21301 tttcggcaat ggtctgaatg atggttggcg taaagacgcg gtgcatcccg gtcattacca
    21361 gcagaggcca cagcgcgccc ataatggcga ctgaaagcca gcccagataa ccatgaatgg
    21421 tgtaaaccag cgccgaaatg gcgctaccga tccagatacc aatcgggcca atcagcagga
    21481 ttgccagcgg tgcggcaatc aacacaatca gcattggctt gaggaagttt ttggtcaccg
    21541 ccggtgtaat gcgatccacc cagcgttcga tatatgacag gcaccaggtc atcaccagcg
    21601 ccgggatcac tgtgtaggtg tatttcaccg cggtgaccgg aatcagggca aattcgacat
    21661 gttcaccctg ggccgctttc gccatcagtt caataaaact cggatgtacc agcacacccg
    21721 cgatggcaat cgccagcgac atattggttt taaatttgat ggcggcagat gccgcgacca
    21781 tcagcggcag gaagaagaaa gcaccgtcac caatcacatt cagaatggtt aaggtcggcg
    21841 atccttttgt cagcacgccg ctcatctcga ggatcatcgc cagcagtttg accatcgatc
    21901 cgccgataat cgccgggatc agcggtgaca tggtgccgat cagcgcatcg aggatccccg
    21961 caccaatgcg acgcagcgtg agtttgggtt tacccacggg ctgtgcgggc tgcatatctc
    22021 ccggcagcag gctgacgact tcctgaaagg cttgtgaaac ggtattgccg ataatcacct
    22081 gacactggtt gtcactacgt accacgccga gcacgccggg gatggttttt aacgtcgggc
    22141 tgtcgataag ctggtcatct ttgataacaa cgcgcaaacg cgtcatacag tgcgtgaccg
    22201 ccgagatgtt atcaacgccg cccagtgccg ctatcaccga gcgtgccagc gccgcataat
    22261 ttttggccat cggattttat cctgttatca gtagggtaga tgtagttgtt atcagtcgac
    22321 gtaacggaaa aatgccgatg ctgaaacgga attaggaaac cggtttcaca aaatcatgaa
    22381 tagttgtcac taaataagca agagagttaa ttgttcattt tttatatttg tgaaaccggt
    22441 cacctgaata tttttcccgc cgattgcgca aggacaatcg gcaaacggtg cagtacactg
    22501 cgttgattgc gtgtaaaggg atgaaaatga tgacgacgat gctggaagtg gcgaagcgcg
    22561 ccggggtttc aaaagcgacc gtttcccgcg tgctttcagg taatggctac gtcagccagg
    22621 agactaaaga tcgcgtgttt caggcggtag aagagagcgg ttaccgccca aacttgctgg
    22681 cgcgcaatct gtcggcgaag agtactcaga cgctggggtt ggtagtgacc aacacgcttt
    22741 accacggcat ttattttagt gaattactct ttcatgccgc gcgaatggcg gaagagaaag
    22801 ggcggcagtt gctattggca gatggtaaac acagcgccga agaagagcgc caggcgattc
    22861 agtatctgct ggatttgcgc tgcgacgcca ttatgattta cccgcgtttt ttaagcgtgg
    22921 atgagatcga tgacatcatt gacgcgcaca gtcagccgat aatggtgctt aatcgccgcc
    22981 tgcgcaaaaa cagcagccat agcgtctggt gcgatcataa acagaccagc tttaatgccg
    23041 tggcagagtt gataaacgcc ggacatcagg agattgcttt ccttaccggc tcgatggatt
    23101 cccccaccag cattgaacgt cttgctgggt ataaagacgc gctggcgcag catggtattg
    23161 cgctcaatga aaaacttatc actaacggta aatggacgcc cgccagcggg gccgaagggg
    23221 tagaaacgtt gctcgaacgt ggggctaaat ttagcgcgtt agttgccagt aacgacgata
    23281 tggcgatagg tgcgatgaaa gcgttgcacg agcgcggcgt agcggtgcca gagcaggtgt
    23341 cagttatcgg attcgatgat atcgcaattg ccccctatat cgttccggcg ctctccagcg
    23401 taaaaattcc ggtgactgag atgattcagg aaattattgg acggctgatt tttatgctcg
    23461 atggcgggga tttctcaccg ccgaaaacct tcagcggaaa actgatccgc cgcgactcac
    23521 tcattgctct ttcgcgataa acattgtcat cacatgtcat cgtcactagt gtcgatgaca
    23581 tcttttttcc tgcattacag aaattaattt ccttaaataa cagtgaatta aaactggcat
    23641 gatttgtgaa tgtatcggcg cattaactgt cattgctgga gaatttgatg aaccgtttca
    23701 tcattgctga cgcgagtaaa tgtattggtt gctgtacctg tgaagtggcg tgcgtggttt
    23761 ctcatcagga aaatcaggac tgtgcatcgc tgaccccgga aacattttta ccgcgtatcc
    23821 atgtcattaa aggtgtgaac atttccacgg cgacagtttg ccgtcagtgt gaagatgcac
    23881 cgtgcgctaa cgtctgcccg aatggtgcta tcagccgtga taaagggttt gttcatgtga
    23941 tgcaggaacg ttgcattggt tgcaaaacct gcgttgtggc ttgcccgtat ggcgcgatgg
    24001 aagtggtggt acgtccggtg attcgcaaca gcggcgcggg gctgaatgta cgggctgaca
    24061 aagccgaagc caataaatgt gacctgtgca accatcgtga agacggcccg gcgtgtatgg
    24121 cggcttgccc gactcatgcg ctgatttgtg tcgatcgtaa taaacttgaa caactgagtg
    24181 cagaaaaacg ccgccgtacg gcgctgatgt tctaaaaatc ttacagaact gttctggcat
    24241 caaaggaatt cccggaaata ttcgtagaat acggagctgg cggtatcgcc gacctgagac
    24301 tgccggatac gacgcagtcg tgtccggcag gacgggttac gcaattcatg atggcgggaa
    24361 taactcaatg gcaaaaaaca catcttgcgg tgtccaactg cgtattcgtg gcaaagtgca
    24421 gggcgtcggt tttcgtccgt ttgtctggca gctggcacag caattaaatc ttcacggcga
    24481 tgtctgtaat gacggcgatg gcgtagaagt ccggctgcgg gaagacccgg aaacgttact
    24541 tgttcaattg catcagcact gcccgccgct ggcgcgtatt gatagcgtcg agcgtgagcc
    24601 gtacatctgg tcacaactgc ccactgagtt caccatccgc cagagcgcgg gaggtgccat
    24661 gaatacgcaa attgtcccgg atgccgctac ttgccctgct tgccttgccg aaatgaatac
    24721 cccaggcgaa cggcgttatc gttatccgtt tatcaactgt acccactgcg gcccgcgttt
    24781 caccattatt cgcgccatgc cttacgaccg cccgtttacc gtgatggcgg cgtttccgtt
    24841 gtgtccggct tgtgacaaag agtaccgtga cccgctcgat cgtcgcttcc acgcccagcc
    24901 ggtggcctgc ccggagtgtg gcccacatct tgaatgggta agtcatggtg aacatgcaga
    24961 acaagaggcg gcattacagg cggctatcgc acagttaaaa atgggcaaca ttgtcgccat
    25021 caaagggatt ggcggatttc atcttgcctg cgatgcacgt aacagtaacg cggtggcgac
    25081 acttcgggcg cgcaaacatc gcccggcgaa accgctggcg gtcatgttgc cagtggctga
    25141 cggtttacca gacgctgcgc gccagttgct taccacgccc gccgcgccga ttgtgctggt
    25201 ggataaaaaa tacgttcctg agctttgtga tgatatcgcc cctgacctta acgaagtcgg
    25261 ggtaatgttg cctgcgaacc cgctccagca tttgctgtta caggaactgc aatgcccgct
    25321 ggtgatgacc tccggcaacc tgagcggtaa accaccagct atcagcaacg aacaggcgct
    25381 ggcggatttg cagggcattg ccgacggatt cttgatacat aaccgcgaca tcgtgcagcg
    25441 gatggatgat tcggtggtgc gcgaaagcgg cgaaatgctg cgccgttcgc gggggtatgt
    25501 gccggatgcg ctggctttgc ctccgggctt taaaaatgtt ccgcctgtgc tgtgtctcgg
    25561 cgcggatctg aaaaatacct tctgcctggt gcgcggtgaa caagcggtgt tgagtcagca
    25621 tctgggcgat ttaagtgacg atggcatcca gatgcagtgg cgcgaagcgt tacgcctgat
    25681 gcaaaacatc tacgatttta ccccgcaata cgttgtgcat gacgcgcatc cgggctatgt
    25741 ctccagccag tgggcgcgtg aaatgaatct gccgacgcaa acggtactgc atcatcatgc
    25801 ccatgcagcg gcgtgtctgg cagagcatca gtggccgttg gatggcggtg atgtcattgc
    25861 tttgacgctc gacggtatcg gtatggggga gaacggcgct ttgtggggcg gcgagtgcct
    25921 gcgggtgaac tatcgcgaat gtgagcacct gggcggcttg cctgcagtgg cgcttccggg
    25981 tggcgatttg gcagcgaagc agccgtggcg aaacctgctg gcgcagtgcc tgcgctttgt
    26041 gccggagtgg cagaattacc ctgaaacagc aagtgtgcaa cagcaaaact ggagcgtgct
    26101 ggcgcgggcc attgagcgtg gaattaacgc gccgctggcg tcatcgtgtg ggcggttgtt
    26161 cgatgcagtg gcggcggcac tgggctgtgc gccagccacg ttaagttatg aaggtgaagc
    26221 ggcttgtgct ctggaggcgc tcgcagcctc atgccacgga gtgacgcatc cggtgacgat
    26281 gccgctggtg gacaatcaac tggatctcgc cgctttctgg cagcagtggc tgaactggca
    26341 ggcaccggtt aatcaacgcg cgtgggcgtt tcatgatgcg ctggcgcagg gttttgccgc
    26401 gttgatgcgt gagcaggcca cgatgcgtgg tatcactacg ctggtattta gcggcggggt
    26461 tattcataac cgtttgctgc gtgcacgtct ggcgcattat ctcgctgatt tcacattgct
    26521 ctttccacag agtttaccgg cgggtgatgg cggtttgtct ctggggcagg gggttattgc
    26581 tgcggcgcgt tggttagcgg gtgaagtcca gaacggataa aacgtgttgc atttggacac
    26641 cattgccgga tgcggcgtaa acgccttatc tggcctacac atgcgtcccc gtaggtcgga
    26701 taagacgcgc cagcgtcgca tccgacattt taggcacagt agcccaccta catcggcaat
    26761 gttttcaaca atccaaatgc ctctttcatc cgatcctcac tgaccacaaa ggcgcgaagc
    26821 tggtcagcat cgtcaacacc gcgagccacc attccctggc gttcggtatt aatttgccag
    26881 cgtaaatcct gacgctgggt ttcgcctgcc agatgcagcg gtaattccgg cgttttgatt
    26941 ttcaccagca tcgccggtag tttcagcggc gtgttattgc cgagaagatt ttttgccagc
    27001 accatcgcgc taagttgaat cggctgcagg aatggcagta cctgaccgtt gatttccgcg
    27061 caatcgccca gcgcataaat atcgtcatta ctggtttgca gataactatc gacgcaaacg
    27121 ccgcgattaa tcgtcagccc ggcgcgtcgt gccagggctg tttccgggcg cagtccggtg
    27181 gcggcaatta ccgcatccac ttcgatacta cgctggtggt ccagcgttgc cagaatgcca
    27241 gaatccgttt tttccagccc ctgcaactga gatttcaaca gcagatgaac gcccatctcc
    27301 gtcaaccgat gctgcaagcg gctgcttact tctggtggca ttaatgacgc cagaatactg
    27361 gcagcgttgt cgattagcgt gaccgctttg cctgcacgac aaaaatccat cgccagttcg
    27421 ctaccaatca aaccaccgcc gacaatcaac acgcgtcggg catcccgcag ttgcgtttca
    27481 caggcgcgat actcttgctg actatttaac gtcagcatta actcacgccc aggcactggc
    27541 gggacaaagg cactggcacc agttgccagc accagcttgt cgtattgcca ctgattattc
    27601 tggcttttca ccacatgggc ttcggcatcg atatccgtca cccaggtgtg cggaaacagg
    27661 tgcagattaa actgcccggc aaattcaccc gccgtctggc gggtaaggtc gtcggcacgt
    27721 tgcccctggc tgataacatg gctgaggtcc ggtttgttgt actcatccat gctgtcggcg
    27781 gcgatcaggg ttaatggaat actggcgtcc tgtttgcgaa tatttttcac cagttggcgg
    27841 gcggcgaagc ccgaaccaat gatcacaatg ccgttgctca ttttgcctcc gatgccagtt
    27901 cgtcaaagac gtctttgccg agagagcatt ccgggcagag gaagttatcc ggaacttcac
    27961 tccacggcgt tcctggcgca acgtcctgca ttggctcgcc ttttgccgga tcgtaaatcc
    28021 actggcagac gctgcactgc atccgtgggc cgaggtcgga cttcgtggtg gcagaggttt
    28081 cttctttaac caccgtattc accgtgctct gcggcagcgg cgcgagcgcc cactgacggg
    28141 cgatttcgcg accatgttca cggcataact ccagagcgtc ctggtctggt cgccatttgg
    28201 ctttcaggct aagcgacatt tcgaaacccg catcctgcag gcgcgtggaa agacgatcca
    28261 ccgcaccgcc gctccagccg tgagagccga aagcactggc gcgtttgtta cggaagcgta
    28321 atccggtcat ctcctccacc agaccggcga ttttcggcat catcacgtta ttcatcgtcg
    28381 aagtgccgac cagcacgcct tttgagcgga agacattggt caggatttcg tttttatcgc
    28441 ttcgggcgac gttgaaaatt ttcaccgcca cgcgtgggtc ggtttccgca atcccctggg
    28501 cgatagcgtc agccatcatg cgggtgttat tcgacatggt gtcgtagaaa atggtgattc
    28561 tgtcttcctg ataatcagcc gcccatttca ggtacagctc gacaatttgc gtcgggttat
    28621 cgcgccatac cacgccgtga gaggtcgcta tcatatcgac tggtaagtta aagcccagga
    28681 tctcggtaat tttcggtgtt accaggcggc tgaacggcgt caggatattg gcgtagtaac
    28741 gctggcactg ctcgaaaagc tccgtctgat ccacttcatc gttgaacaga tgctcgtcgc
    28801 agtagtgctg accgaaagca tcgttactga acagcaccgc gtcgcctgtc aggtaagtca
    28861 tcatgctgtc cggccagtgc agcattggcg tttcgacaaa aatgagctgt ttgccgttgc
    28921 cgatatccag cgtgtcacca gttttcacca cattaaaatt ccactccgga tgatgatgat
    28981 gaccatttat cgagtcgata gcgttggcag tacagtaaat cggcgtatcg ggaatttgcg
    29041 ccatcagttc ggtcagcgcc ccagcgtggt cctcttctgc atggttaatc acgatgtaat
    29101 cgatatccgc cagatcgatt tcattacgca ggttctgcac aaattcgcgg ctgaatttat
    29161 ggtcgacggt gtcgatcagc acgttttttt cttcgcggat gaggtagctg ttgtaactgc
    29221 tgccgcgtag cgttttatat tccgtaccgt gaaaatcacg cacttcccag tcacgttgac
    29281 caacccaatg aatgttattt ttcaccacaa tagacatagc aacctcaatt tattcagcgt
    29341 gttctaaaaa gatgtcttgc taattgcaga ttacgtgcca gttttttatc tgattgtttt
    29401 tactctataa ataaaattat gctcattaat gagtagtcaa aatgactatc aatcaaatgg
    29461 tcgatatgac aatatctata gtcaaattga cagtgaggca aagatgagtt tttccgttga
    29521 tgtgctggcg aatatcgcca tcgaattgca gcgtgggatt ggtcatcagg atcgttttca
    29581 gcgcctgatc accacgctac gtcaggtgct ggagtgcgat gcgtctgcgt tgctacgtta
    29641 cgattcgcgg cagtttattc cgcttgccat cgacggtctg gcaaaggatg tactcggtag
    29701 acgctttgcg ctggaagggc atccacggct ggaagcgatt gcccgcgccg gggatgtggt
    29761 gcgctttccc gcagacagcg aattgcccga tccctatgac ggtttgattc ctgggcagga
    29821 gagtctgaag gttcacgcct gcgttggtct gccattgttt gccgggcaaa acctgatcgg
    29881 cgcactgacg ctcgacggga tgcagcccga tcagttcgat gttttcagcg acgaagagct
    29941 acggctgatt gctgcgctgg cggcgggagc gttaagcaat gcgttgctga ttgaacaact
    30001 ggaaagccag aatatgatgc caggcgatgc cacgccgttt gaagcggtga aacagacgca
    30061 gatgattggc ttgtcccctg gcatgacgca actgaaaaaa gagattgaga ttgtggcggc
    30121 gtccgatctc aacgtcctga tcagcggtga gacgggaacc ggtaaggagc tggtggcgaa
    30181 agcgattcat gaagcctcgc cacgggcggt gaatccgctg gtctatctca actgtgctgc
    30241 actgccggaa agtgtggcgg aaagtgagtt gttcgggcat gtgaaaggag cgtttactgg
    30301 cgctatcagt aaccgcagcg ggaagtttga aatggcggat aacggcactc tgtttctgga
    30361 tgagatcggc gagttgtcgt tggcattgca ggccaagctg ctgagggtgt tgcagtatgg
    30421 cgatattcag cgcgttggcg atgaccgtag tttgcgggtc gatgtgcgcg tgctggcggc
    30481 gactaaccgc gacttacgcg aagaggtgat ggcagggcga tttcgcgctg acttgtttca
    30541 tcgcctgagc gtgtttccac tttcggtgcc gccgctgcgt gagcggggcg atgatgtcat
    30601 tctgctggcg gggtatttct gcaagcagtg tcgtttgcgg ctggggctct cccgcgtggt
    30661 attaagtgcc ggagcgcgaa atttactgca acactatcgt tttccgggga acgtgcgcga
    30721 actggaacat gctattcatc gggcggtagt gctggcgaga gccacccgca acggcgatga
    30781 agtgattctt gaggcgcaac attttgcttt tcctgaggtg acgttgccgc cgccagaagc
    30841 ggcggcggtg cccgttgtta agcaaaacct gcgtgaagcg acagaagcgt tccagcgtga
    30901 aactattcgc caggcactgg cacaaaatca tcataactgg gctgcctgcg cgcggatgct
    30961 ggaaaccgac gtcgccaacc tgcatcggct ggcgaaacgt ctgggaatga aggattaaat
    31021 aatcccggcc tgatagaaat cctgcaggtt aattgcgccg gtgagtttgc cgttttcatc
    31081 caccaccggt gcggcagtga ttttgcgctt catcaggatc tctttggcgt cgatggcgcg
    31141 actttgcgcc tgtaacgtgg tgccgcctgt cgtcatcgct tcattaactg gcgtggtgag
    31201 tgcgccgccg cctaccagcc agcgacgtaa atcgccgtcg gtaaagacgc cttgtacctg
    31261 ttgttgagcg tcacataccg ccaccagccc cagaccggtg cggctgagtt ccagcatcgc
    31321 atccatcacg ctggcggtta acgccacctg tgggatggca tcgtcacggc gcatcagatg
    31381 atgcacttta ttcagcaagc gagcgcccag tgccccggct gggtgggagc gggcaaaatc
    31441 ttcttcatta aatccgcgcg cctgcatgac cgccatcgcc agcgcgtcac ccatcatcag
    31501 ggtattgacg gtgctggagg tcggcgcaag gtgcatcggg caggcttcgc gttctacgga
    31561 gatatccagc accgcttttg ccgccaggcc cagcggtgac gtcggtttgc cggtcatcgc
    31621 cagcagcgcg atagatttat cttccagacg cggaataatc agatccagtt ccttcgcgcc
    31681 accggagtaa gagataaaca gcatcacatc gcggctttca atcatcccca gatcgccgtg
    31741 cagtgcttct gccggatgga caaaaaaagc cgatgtgccg gtactggcaa gtgttgcggc
    31801 gattttctta ccaatgtggc ccgatttgcc aattcccgaa accaccactt tgccttcgca
    31861 gtgcaggatg atattggcgg cgcgaacaaa atcatcgccc agacgttccg gtaaacggct
    31921 tgcttcctgc aactccagca ttaacgtctg acgtcccgcg ttcagtagtg cttcactcat
    31981 tgctctctcc ggttatgatc acatcgatcc ctttctcttc cagcgcctga cgaaacgccg
    32041 gatcgatacc tgcgtcggta atcagcttat cgacgctttc aagactgcaa actacgttgg
    32101 ggcttttacg gccaaacttt gatgagtccg ccatcaaaat cacttcgcgc gcggcattgc
    32161 acattgcctt actgacggta taaacctcgt taaaggtggt tacgcccgca ttgagatcga
    32221 tgccgtcggt gcccataaac aatttatcga aactgaaatg ttcgaaggca ttctctgcca
    32281 gctgtccgtg aaatgaggcc gattttttac gaaacgttcc gcctggcatc aggatagttt
    32341 gttcgttatc cagttcagat agcgcattga cgatatgcag actgttggtc atcaccgtga
    32401 tgttattaaa gcgcgagagc aggggaacca tctgcaaaac ggtactgcca gcatcaagaa
    32461 tgatcgaatc gccatcatga ataaaactaa cggcagcttc tgctatcagc tctttcttgt
    32521 gggtgttgat gagtgtttta tgatcgatag gcggatcgga ttcctcttta ttcaacacca
    32581 ctccgccata agtacgaatg acggttcctg catgttccag aatgaccaga tctttgcgaa
    32641 tggttgtgcc tgtggtgtca aagtattgcg ccaattcttc aaccgagcat ttaccctgct
    32701 tttgcagata ctccagaatg gcggcctgac gctgacgagg tttcatgggc gtgattacct
    32761 gatttaggtt tcaaaatgat aacttcgcaa gcaagtaaca ttcacaacga aattatccac
    32821 gtttcagttt aagcgccaat gatagagcat tctgtgatag tggatcatgg ggaaagatca
    32881 catcgggctg taaatcaccc gcatgcatac cggtaattgc gggaattttt tgcggtcgtg
    32941 cgaagacggt cagtcctttc ataaacaagg tgtcgacgat gcgctgctga tcgtccaggg
    33001 cgatggcgac cacgacccgt ggtttaaagc gcccgctgga acggcccacg ccaacccgcc
    33061 cttgctggca tagtgtgtcg aaggcacggt taaaacgaga aatttgccag ccgcctaagg
    33121 ccagttgcgc acaccaggcg ataacggcga cggtgatgag tgcggatacc ataacttttc
    33181 cttaatgtag tggcggaagt cctgtatgcc tgatgagcgt agcgcatcag gcattctatt
    33241 agtctcacgg agatccttaa cggatctccg ctgttgatta caacatcacc tgaccgccgg
    33301 tgacattgat cgactgtccg gtgcagtacg acgccttagg actggcatag agcagcagca
    33361 tattcagcac atcctgataa tcgcagccgc gtttgagcgg tactttgtcg atgtaatact
    33421 gctcgacttg atccggtttg atacccagct tggtcgcgta ttggggtaac agtgactgga
    33481 acataggcga tttcagcagg ttaccgagca tcagtgaatg caccgtaatg ccgtactccg
    33541 ccagatccag cgccagtgat tgagtcagcc cgacgccacc aaatttcgct gcgctgtagc
    33601 cagagttgtg tttgctgccc actttgccgg atttcgagtt gatctgaata atgcgaccct
    33661 gaatcccgtc gcggatcatc aaacgagaaa attcacgggc acacaggaaa taacccacca
    33721 gattcacggt caaaatcgcc gagctggaag tcgctgataa aggctgcttt tgctattccg
    33781 gcgctgtaga ccagcaaatc cacgcgccca aagatttcat ctaccccacg agagagcgcc
    33841 aggacgcttt gctcgctggt ggcgtcagca ccaaaactat acgctgttcc ttcaccatat
    33901 tcggcgttaa tttcttgtgc cacatttgcg gctttgtcgc tctgaatatc gacaaccgcg
    33961 acgcgatacc cctcggcagc cagaccgtgg cacaggaacg cgcctaaggt ttgcccacca
    34021 ccgatgacaa cggcaacctg attcattttt tactccttaa cagattcaaa cttcaaaacc
    34081 gatcccggcg cgatatcgtc gggtacaggg cctgccacat ggacagtgcc cggaaattcc
    34141 gcttcgttta aaccatcgaa gcgcagggtg acatgaccca gttcgcgaag gttgtcttcc
    34201 gccacgctgc caacagcggt caccggatag cgatgctgcc cgagcgtaaa ttgcaaaccg
    34261 ggatggagtg cacctttcaa ctcgccgtgg caatgaatga agcaatactc ttcgaggtcc
    34321 gctggcgcgc cttcacgaaa ggtgatgagc atctggtcgc tgagggcgtc agtggcactc
    34381 gcgccgatac gggtgatggt ggtctgataa ataacggtca tgtttcagcc tctattgata
    34441 gataaaaccg gagacaaacc aggcgatcag tacagtcggt gcgccggtta aaaagtggct
    34501 caccagtaca gaagggacac cgacgcgaac cgtgtcctga cgggcttccg ccagcgacaa
    34561 accgaccggg atgaagtcgc aggccgcctg cgcgttgatg gcaaacagtg ccggtaaagc
    34621 cagatgcggc ggaatattgc cgagaccaat ctgcacgcca atcaatacgc cgataacctg
    34681 tgcgataact gcgcctgggc cgaggaaagg tgaaagcagc gggaaggagc agatgagcgc
    34741 cagcatgacc agacctagtg gatggctcgc cagcggagca agaccgtggg caatccagtc
    34801 accaaggcca gaagccataa tgatgccaat gagcgccgag acgaacgcca taaacggcag
    34861 aatggttttt aatacagtgt cgatggtgtc acgaccagat tgaaacaaca cggcaacggc
    34921 agaacccata cccattccca ccttcgccag taaaccatcg ctttgttcgg tgattttctt
    34981 gctggtgtca tagtcacggc ccacggaaga gggttgtggt gtcgcatcac ccactacagt
    35041 gatgttttct tcttttacgc cagagacata aatatcttcc acaatgtact gcgccagcgg
    35101 actggacttg cccgtcgagt ggatattaat ggtgggaata cgtcgtttcg gatagatgcc
    35161 gcagcgtaat gtgccgccac agtcgattac cgcgacacca atttccgcct ccgcgggttc
    35221 accttcttta aatccgtcaa tagcctgcca gccagtaagt tgtgccagtt tgtcaacaat
    35281 cgcaggccgg gtaccggcgg tgatatagac gatttttttg cccggcgtgg cttccagctc
    35341 aagcgggccg ccccagccac ccgttccttt ttcgatccga atacgcgtca tgatgttgct
    35401 cctgccaggt gaactttttg ttcaagttga atgcccatct ttttctcaaa aatggcggtg
    35461 gtcagatcgg tcacccagcc gcgaaagaag ttggtgacca gaccaaccag cagatagctc
    35521 accgccagtg ggccaagtgg caggttcagc gttgtcagac cgctggcaat gccaagataa
    35581 acaaacagtt cgccagggtt gatatgggga aagaggccat tcattgagtg gcagctataa
    35641 gaggctgccg cgtagtagct gggtttgtac ttttccggca taaagcgacc caggcttaag
    35701 gtcatcggat tgcaaaagac aaacgtgcca atgcacggta gcagcaggta acgggaaaca
    35761 gggttaccgg cgcaacgttg agcaaaacgt tcaatacgat gctgaccgat aaaattaatc
    35821 agtgcgttca tgataaccag caggctaatc aacagcggaa gaatgccggt caccatcccg
    35881 gtaaacacct ctccgccctt ttggaacagc ccgataaacc actctgcacc atgagtaatg
    35941 gtttctatca ttgttctctc cttcaggatt tattgtttta ttaccaaacg gcaacctaat
    36001 ctaatcagat tgaaagattt gaaagtgtta ttttgatcgc aaaatgaaag ataaatattt
    36061 taatttgaaa gtttgaataa aaggatagcg aggggaatga gttgagttat gtaaagtccg
    36121 tatcgggcag tgactaccgc ttccttgtgc ggggcgtgat gctttaccat acttgcccct
    36181 ggttgaatct gttaaatgga cccctcatgt tcaagcgtcg ttatgtaaca ttgcttcccc
    36241 tttttgtgtt gcttgccgcc tgtagcagca agccaaaacc tactgagact gaaacgacca
    36301 ccggaacgcc gtctggcggc ttcctgcttg agcctcagca caatgtgatg cagatgggcg
    36361 gcgatttcgc taataacccg aatgcccagc agttcatcga cagaatggtg aacaaacacg
    36421 gtttcgatcg tcagcagttg caggaaattc tctcccaggc gaagcgtctg gattcggtac
    36481 tgcggctgat ggataaccag gcaccaacca catcggtgaa accgccatca ggtccgaacg
    36541 gcgcatggct gcgttatcgc aaaaaattta ttacgccgga caacgtgcag aacggtgtgg
    36601 ttttctggaa tcagtatgaa gatgcgttga atcgcgcgtg gcaggtgtat ggcgtaccgc
    36661 cggaaattat cgtcgggatt atcggcgttg aaacccgctg ggggcgcgtg atggggaaaa
    36721 ctcgcatcct cgatgcgctg gcaacgctgt catttaacta cccacgccgc gcggagtatt
    36781 tctctggcga gctggaaacc ttcctgttga tggcgcgcga cgagcaggac gatccgctca
    36841 atctgaaagg ttcctttgcc ggggcgatgg gctacggaca gtttatgccg tcgtcttaca
    36901 aacaatatgc ggtagatttc agcggcgacg ggcatatcaa cctgtgggat ccggttgatg
    36961 cgatcggtag tgtggcgaac tatttcaaag cgcacggctg ggtgaaaggc gatcaggtcg
    37021 cggtaatggc aaacggtcag gctccaggct tgccaaatgg cttcaaaacc aggtacagca
    37081 tttcgcagct tgccacggca ggtttaacgc cacagcagcc gctgggcaac catcaacaag
    37141 ccagcctgct gcgtctggat gtcggtaccg gttaccagta ctggtacggt ctgccgaact
    37201 tctacaccat tacccgttat aaccacagca cccattacgc aatggcggtc tggcagttag
    37261 gacaagccgt ggcgctggcg cgcgtgcagt aactatttgc aagagggggg cgtgagcttc
    37321 cctctggcta atcagctttt ttctgaatcc tcctcgtaaa attgcaacgc caacaccatc
    37381 ttcctgacga aagtgctatc ttgtccggca taaatttttg actgactgag gttgtgatga
    37441 ctgacagtga actgatgcag ttaagcgaac aggttgggca ggcgctgaaa gcccgtggcg
    37501 caaccgtaac aactgccgag tcttgtaccg gtggttgggt agcgaaagtg attaccgata
    37561 ttgccggtag ctccgcctgg tttgaacgcg gatttgtcac ctacagtaac gaagccaaag
    37621 cgcagatgat cggcgtacgc gaagagacgc tggcgcagca tggcgcggtg agtgaacccg
    37681 tcgtggtgga aatggcgata ggtgcactga aagcggctcg tgctgattat gccgtgtcta
    37741 ttagtggtat cgccgggccg gatggcggca gtgaagagaa gcctgtcggc accgtctggt
    37801 ttgcttttgc cactgcccgc ggtgaaggca ttacccggcg ggaatgcttc agcggcgacc
    37861 gtgatgcggt gcgtcgtcag gctactgcgt atgcattgca gaccttgtgg caacaatttc
    37921 tacaaaacac ttgatactgt atgagtatac agtataattg cttcaacaga acatattgac
    37981 tatccggtat tacccggcat gacaggagta aaaatggcta tcgacgaaaa caaacagaaa
    38041 gcgttggcgg cagcactggg ccagattgag aaacaatttg gtaaaggctc catcatgcgc
    38101 ctgggtgaag accgttccat ggatgtggaa accatctcta ccggttcgct ttcactggat
    38161 atcgcgcttg gggcaggtgg tctgccgatg ggccgtatcg tcgaaatcta cggaccggaa
    38221 tcttccggta aaaccacgct gacgctgcag gtgatcgccg cagcgcagcg tgaaggtaaa
    38281 acctgtgcgt ttatcgatgc tgaacacgcg ctggacccaa tctacgcacg taaactgggc
    38341 gtcgatatcg ataacctgct gtgctcccag ccggacaccg gcgagcaggc actggaaatc
    38401 tgtgacgccc tggcgcgttc tggcgcagta gacgttatcg tcgttgactc cgtggcggca
    38461 ctgacgccga aagcggaaat cgaaggcgaa atcggcgact ctcacatggg ccttgcggca
    38521 cgtatgatga gccaggcgat gcgtaagctg gcgggtaacc tgaagcagtc caacacgctg
    38581 ctgatcttca tcaaccagat ccgtatgaaa attggtgtga tgttcggtaa cccggaaacc
    38641 actaccggtg gtaacgcgct gaaattctac gcctctgttc gtctcgacat ccgtcgtatc
    38701 ggcgcggtga aagagggcga aaacgtggtg ggtagcgaaa cccgcgtgaa agtggtgaag
    38761 aacaaaatcg ctgcaccgtt taaacaggct gaatttcaga tcctctacgg cgaaggtatc
    38821 aacttctacg gcgaactggt tgacctgggc gtaaaagaga agctgatcga gaaagcaggc
    38881 gcgtggtaca gctacaaagg tgagaagatc ggtcagggta aagcgaatgc aactgcctgg
    38941 ctgaaagata acccggaaac cgcgaaagag atcgagaaga aagtacgtga gttgctgctg
    39001 agcaacccga actcaacgcc ggatttctct gtagatgaca gcgaaggcgt agcagaaact
    39061 aacgaagatt tttaatcgtt cgttattgat gataatagat aatgtgtgca cggacggtcc
    39121 cctcgcccct ttggggagag ggttagggtg aggggaacag gccaacactg gcgtgaaaaa
    39181 ttactctcac ctcgatcctc taacgaattg gctgcacact cattgactat taaatctgtt
    39241 tatcagcaaa acctgggtcg catctgcggc ccttttgctt ttttaagttg taaggatatg
    39301 ccatgacaga atcaacatcc cgtcgcccgg catatgctcg cctgttggat cgtgcggtac
    39361 gcattctggc ggtgcgcgat cacagtgagc aagaactgcg acgtaaactc gcggcaccga
    39421 ttatgggcaa aaatggccca gaagagattg atgctacggc agaagattac gagcgcgtta
    39481 ttgcctggtg ccatgaacat ggctatctcg atgacagccg atttgttgcg cgctttatcg
    39541 ccagccgtag ccgcaaaggt tatggacctg cacgtattcg tcaggaactg aatcagaaag
    39601 gtatttcccg cgaagcgaca gaaaaagcga tgcgtgaatg tgacatcgac tggtgcgcac
    39661 tggtgcgcga tcaggcgacg cgaaaatatg gtgaaccttt gccaactgtc ttttcagaaa
    39721 aagttaagat ccagcgtttt ctgctctatc gtggctatct gatggaagat atccaggata
    39781 tttggctaaa ttttgccgac tgaacgcata cggtatttta cttcccactc aagaaaactt
    39841 atcttattcc cacttttcag ttaccagccc ggcggttaag acacgctgga gctggtggcg
    39901 atatttcgtt agcttgattt caggataatt atgagcaaga gcaccgctga gatccgtcag
    39961 gcgtttctcg actttttcca tagtaaggga catcaggtag ttgccagcag ctccctggta
    40021 ccccataacg acccaacttt gttgtttacc aacgccggga tgaaccagtt caaggatgtg
    40081 ttccttggac tcgacaagcg taattattcc cgcgctacca cttcccaacg ctgcgtgcgt
    40141 gcgggtggta aacacaacga cctggaaaat gtcggttaca ccgcgcgtca ccataccttc
    40201 ttcgaaatgc tgggcaactt cagcttcggc gactatttca aacacgatgc cattcagttt
    40261 gcatgggaac tgctgaccag cgaaaaatgg tttgccctgc cgaaagagcg tctgtgggtt
    40321 accgtctatg aaagcgacga cgaagcctac gaaatctggg aaaaagaagt agggatcccg
    40381 cgcgaacgta ttattcgcat cggcgataac aaaggtgcgc catacgcatc tgacaacttc
    40441 tggcagatgg gtgacactgg tccgtgcggc ccgtgcaccg aaatcttcta cgatcacggc
    40501 gaccacattt ggggtggccc tccgggaagt ccggaagaag acggcgaccg ctacattgag
    40561 atctggaaca tcgtcttcat gcagttcaac cgccaggcag atggcacgat ggaaccgctg
    40621 ccgaagccgt ctgtagatac cggtatgggt ctggagcgta ttgctgcggt gctgcaacac
    40681 gttaactcta actatgacat cgacctgttc cgcacgttga tccaggcggt agcgaaagtc
    40741 actggcgcga ccgatctgag caataaatcg ctgcgcgtaa tcgctgacca cattcgttct
    40801 tgtgcgttcc tgatcgcgga tggcgtaatg ccgtccaatg aaaaccgtgg ttatgtactg
    40861 cgtcgtatca ttcgtcgcgc agtgcgtcac ggcaatatgc tcggcgcgaa agaaaccttc
    40921 ttctacaaac tggttggtcc gctgatcgac gttatgggct ctgcgggtga agacctgaaa
    40981 cgccagcagg cgcaggttga gcaggtgctg aagactgaag aagagcagtt tgcccgtact
    41041 ctggagcgcg gtctggcgtt gctggatgaa gagctggcaa aactttctgg tgatacgctg
    41101 gatggtgaaa cggctttccg tctgtacgac acctatggct tcccggttga cctgacggct
    41161 gatgtttgtc gtgagcgcaa catcaaagtt gacgaagctg gttttgaagc agcaatggaa
    41221 gagcagcgtc gtcgcgcgcg cgaagccagc ggctttggtg ccgattacaa cgcaatgatc
    41281 cgtgttgata gtgcatctga atttaaaggc tatgaccatc tggaactgaa cggcaaagtg
    41341 accgcgctgt ttgttgatgg taaagcggtt gatgccatca atgcaggcca ggaagctgtg
    41401 gtcgtgctgg atcaaacgcc attctatgcg gaatccggcg gtcaggttgg tgataaaggc
    41461 gaactgaaag gcgctaactt ctccttcgcg gtggaagata cgcagaaata cggccaggcg
    41521 attggtcaca tcggtaaact tgctgcgggt tctctgaaag tgggcgacgc tgtgcaggct
    41581 gatgttgatg aggctcgtcg cgcccgtatt cgtctgaatc actccgcaac gcacctgatg
    41641 cacgctgcgc tgcgccaggt tctggggact catgtatcgc agaaaggttc actggttaac
    41701 gacaaggtgc tgcgcttcga cttctcacac aacgaagcga tgaaaccgga agagattcgt
    41761 gcggtcgaag acctggtgaa cgcacagatt cgtcgcaatt tgccgatcga aaccaacatc
    41821 atggatctcg aagcggcgaa agcgaaaggt gcgatggcgc tgttcggcga gaagtatgat
    41881 gagcgtgtac gcgtgctgag catgggcgat ttctccaccg agctgtgtgg cggtactcac
    41941 gccagccgca ctggtgatat tggtctgttc cgcatcatct ctgaatcggg tactgctgca
    42001 ggcgttcgtc gtatcgaagc ggtaaccgga gaaggcgcta tcaccaccgt tcatgcagac
    42061 agcgatcgct taagcgaagt cgcgcatctg ctgaaaggcg atagcaataa tctggcggat
    42121 aaagtgcgct cagtactgga acgtacgcgt cagttagaaa aagaactaca acagcttaaa
    42181 gaacaagctg ccgcacagga gagcgcaaat ctttccagta aggcaattga tgttaatggt
    42241 gttaagctgt tggttagcga gcttagcggt gttgagccga aaatgttgcg taccatggtt
    42301 gacgatttaa aaaatcagct ggggtcgaca attatcgtgc tggcaacggt agccgaaggt
    42361 aaggtttctc tgattgcagg cgtatctaag gacgtcacag atcgtgtgaa agcaggggaa
    42421 ctgattggta tggtcgctca gcaggtgggc ggcaagggtg gtggacgtcc tgacatggcg
    42481 caagccggtg gtacggatgc tgcggcctta cctgcagcgt tagccagtgt gaaaggctgg
    42541 gtcagcgcga aattgcaata atataagcgt caggcaatgc cgtggactcg cttcacggca
    42601 ttcgcattaa cgctatcgac aacgataaag tcaggttgaa gttgtgtata tcggctaaac
    42661 ttaggtttaa cagaatgtaa tgccatgact gcttacatgt aatgtgtttg tcattgctta
    42721 ctttttggcg ttatatgatg gataatgccg ggatacagag agacccgact cttttaatct
    42781 ttcaaggagc aaagaatgct gattctgact cgtcgagttg gtgagaccct catgattggg
    42841 gatgaggtca ccgtgacagt tttaggggta aagggcaacc aggtacgtat tggcgtaaat
    42901 gccccgaagg aagtttctgt tcaccgtgaa gagatctacc agcgtatcca ggctgaaaaa
    42961 tcccagcagt ccagttacta atctttccgc gtctcatctt tatcggtgag acgcaccctc
    43021 aaaatttctc cttcactcta tagtcttttc gctttactcc cgttcattca acttaagtct
    43081 ccattttttt gcattactgc tatctgccag acctccgttt ttctgtagat aaaacactct
    43141 ttttgacgtt tttacagact aattgagcgt gaagtgtgca aacgataaaa gtgtgggaaa
    43201 aattgtttga cttataagtc tcagaaagta atatgtgcgc cacgcagcga cgatgagcga
    43261 taaacaagtt cttcgaagca ctcgtaagag gcgtgtggtg aggtggccga gaggctgaag
    43321 gcgctcccct gctaagggag tatgcggtca aaagctgcat ccggggttcg aatccccgcc
    43381 tcaccgccat ttgcatccgt agctcagctg gatagagtac tcggctacga accgagcggt
    43441 cggaggttcg aatcctcccg gatgcaccat ctcttacttg atatggcttt agtagcggta
    43501 tcaatatcag cagtaaaata aattttcccg atgcatccgt agctcagctg gatagagtac
    43561 tcggctacga accgagcggt cggaggttcg aatcctcccg gatgcaccat attctccgaa
    43621 atcttcagca atgaaggtat cgaagaggta gcggaattaa ccgcaggcac cagggatgat
    43681 aacgttgctt tagcaacggc ccgaagggcg agccgcaagg cgagtaatcc tcccggatgc
    43741 accatctctt aattgatatg gctttagtag cggtatcaat atcagcagta gaataagttt
    43801 tcccgatgca tccgtagctc agctggatag agtactcggc tacgaaccga gcggtcggag
    43861 gttcgaatcc tcccggatgc accatattct ccgtaacctt cagcaatgaa ggtatcgaag
    43921 agatagcggt attaaccgca ggcaccagga aggataacgt tgcgccagca acggcccgaa
    43981 gggcgaacgt cagtgagtca tcctcccgga tgcaccatct tctccaaaaa atttccttca
    44041 aaaatatctc atttctaaat aactccttta tccatttcct gaattttctt ctccttcgcc
    44101 atcagcgaca aaatcaagca attacgctaa agtagcgtca tatttttcga cttgcgagaa
    44161 gaccatgtac gagcgttatg caggtttaat ttttgatatg gatggcacaa tcctggatac
    44221 ggagcctacg caccgtaaag cgtggcgcga agtattaggg cactacggtc ttcagtacga
    44281 tattcaggcg atgattgcgc ttaatggatc gcccacctgg cgtattgctc aggcaattat
    44341 tgagctgaat caggccgatc tcgacccgca tgcgttagcg cgtgaaaaaa cagaagcagt
    44401 aagaagtatg ctgctggata gcgtcgaacc gcttcctctt gttgatgtgg tgaaaagttg
    44461 gcatggtcgt cgcccaatgg ctgtaggaac ggggagtgaa agcgccatcg ctgaggcatt
    44521 gctggcgcac ctgggattac gccattattt tgacgccgtc gtcgctgccg atcacgtcaa
    44581 acaccataaa cccgcgccag acacattttt gttgtgcgcg cagcgtatgg gcgtgcaacc
    44641 gacgcagtgt gtggtctttg aagatgccga tttcggtatt caggcggccc gtgccgcagg
    44701 tatggacgct gtggacgttc gcttgctgtg agtgaagcgt tatcgctttt ctcgttgttt
    44761 gccagtagtt ttctcagcgc tacactatta cccggcaact ccgaagtcgt gctggtggca
    44821 atgttgcttt ccgggatcag tcatccctgg gttttagttt taacagcaac aatgggtaat
    44881 agccttggag ggttaactaa cgttatcctt gggcgtttct ttccattgcg taaaacatcg
    44941 cgctggcaag agaaagctat cggctggctg aaacgctatg gtgcagtcac gctattatta
    45001 agctggatgc ccgtggttgg cgatttactg tgtctgttag cgggatggat gcgcatctcg
    45061 tggggaccgg taatcttttt tttgtgcctt ggtaaagcgt tacgctatgt tgcagttgca
    45121 gcagcgaccg ttcagggcat gatgtggtgg cactaattgt aggcctgcac atatggtcac
    45181 cattacagtt atgctaatta aaacgatttt gacaggcggg aggtcaattt gatcccggac
    45241 gtatcacagg cgctggcctg gctggaaaaa catcctcagg cgttaaaggg gatacagcgt
    45301 gggctggagc gcgaaacttt gcgtgttaat gctgatggca cactggcaac aacaggtcat
    45361 cctgaagcat taggttccgc actgacgcac aaatggatta ctaccgattt tgcggaagca
    45421 ttgctggaat tcattacacc agtggatggt gatattgaac atatgctgac ctttatgcgc
    45481 gatctgcatc gttatacggc gcgcaatatg ggcgatgagc ggatgtggcc gttaagtatg
    45541 ccatgctaca tcgcagaagg tcaggacatc gaactggcac agtacggcac ttctaacacc
    45601 ggacgcttta aaacgctgta tcgtgaaggg ctgaaaaatc gctacggcgc gctgatgcaa
    45661 accatttccg gcgtgcacta caatttctct ttgccaatgg cattctggca agcgaagtgc
    45721 ggtgatatct cgggcgctga tgccaaagag aaaatttctg cgggctattt ccgcgttatc
    45781 cgcaattact atcgtttcgg ttgggtcatt ccttatctgt ttggtgcatc tccggcgatt
    45841 tgttcttctt tcctgcaagg aaaaccaacg tcgctgccgt ttgagaaaac cgagtgcggt
    45901 atgtattacc tgccgtatgc gacctctctt cgtttgagcg atctcggcta taccaataaa
    45961 tcgcaaagca atcttggtat taccttcaac gatctttacg agtacgtagc gggccttaaa
    46021 caggcaatca aaacgccatc ggaagagtac gcgaagattg gtattgagaa agacggtaag
    46081 aggctgcaaa tcaatagcaa cgtgctgcag attgaaaacg aactgtatgc gccgattcgt
    46141 ccaaaacgcg ttacccgcag cggcgagtca ccttctgatg cgctgttacg tggcggcatt
    46201 gaatatattg aagtgcgttc gctggacatc aacccgttct cgccgattgg tgtagatgaa
    46261 cagcaggtgc gattcctcga cctgtttatg gtctggtgtg cgctggctga tgcaccggaa
    46321 atgagcagta gcgaacttgc ctgtacgcgc gttaactgga accgggtgat ccttgaaggt
    46381 cgcaaaccgg gcctgacgct gggtatcggc tgcgaaaccg cgcagttccc gttactgcag
    46441 gtgggtaaag atctgttccg cgatctgaaa cgcgtcgcgc aaacgctgga tagcattaac
    46501 ggcggcgacg cgtatcagaa agtgtgtgat gaactggttg cctgcttcga taatcccgat
    46561 ctgactttct ctgcccgtat cttaaggtct atgattgata ctggtattgg cggaacaggc
    46621 aaagcgtttg cagaagtcta ccgtaatctg ctgcgtgaag agccgctgga aattctgcgc
    46681 gaagaggatt ttgtagccga gcgcgaggca tctgaacgcc gtcagcagga aatggaaacc
    46741 gctgataccg aaccgtttgc ggtgtggctg gaaaaacaca cctgacagaa aagaaaaagg
    46801 ccactcgtga gtggccaaaa tttcatctct gaattcaggg atgatgataa caaatgcgcg
    46861 tctttcatat actcagactc gcctgggaag aaagagttca gaaaattttt aaaaaaatta
    46921 ccggaggtgg ctaaatgccg ttgttagata gcttcacagt cgatcatacc cggatggaag
    46981 cgcctgcagt tcgggtggcg aaaacaatga acaccccgca tggcgacgca atcaccgtgt
    47041 tcgatctgcg cttctgcgtg ccgaacaaag aagtgatgcc agaaagaggg atccataccc
    47101 tggagcacct gtttgctggt tttatgcgta accatcttaa cggtaatggt gtagagatta
    47161 tcgatatctc gccaatgggc tgccgcaccg gtttttatat gagtctgatt ggtacgccag
    47221 atgagcagcg tgttgctgat gcctggaaag cggcaatgga agacgtgctg aaagtgcagg
    47281 atcagaatca gatcccggaa ctgaacgtct accagtgtgg cacttaccag atgcactcgt
    47341 tgcaggaagc gcaggatatt gcgcgtagca ttctggaacg tgacgtacgc atcaacagca
    47401 acgaagaact ggcactgccg aaagagaagt tgcaggaact gcacatctag tcagtaaact
    47461 atcttcacaa ttaattgaaa aaagccagtt caaatgaact ggcttagttg tacttagtgc
    47521 gcaccgccgc caccgccacc tgcgccaaat ggcggtttag caaaccacac cagccccagc
    47581 agtacgagga atatcccggc tgacatccag aagatctcat tggcggaaat aatcagcccc
    47641 tgattggtga tctgctgggc aatccagcct gatgcctgct gttgcgtcat cccaagccct
    47701 tccagttgac tgtacatcgc ctgggcattc gggttgaacg ggtttaccga ctcagtcaac
    47761 tgcgcatggt gcatcgactc gcggttggtc cacatggtcg tggttatcga cgtgccgata
    47821 gaccccgcca gcgttcgcgt aaagttagag aggctcgatg ccgccgccag tcgttccggt
    47881 ggcaaaccag acagcgtaat ggtggtcagc ggcataaaga agcaggccac cgcaaacccc
    47941 tggataaact gcggccaggc cgacgcgcca aaatccatac ctggttcaaa ggtataggca
    48001 cgccagtaga agcagacggc atacataata aagctgaagg ttaccagccg ccgcatatcc
    48061 agtttatgcg cgaagcggcc gataatcggc gacaggatca ccggaataat ccctaccggc
    48121 gcagaggcca aacctgccca ggtcgccgtg taaccgtaga cctcctgcaa caactgcggc
    48181 agcagaacaa tagcgccgaa gtagagcata tacgcgaggc tgatacacaa gcagccgatg
    48241 gtgaagttgc gcgacttaaa caacgacaga tcgactatcg ggttatcgtc ggtcagctcc
    48301 cagacaatca ggaagcagat agccaccacc gccaccacgg taaggatgat aatttcctgt
    48361 gatgaaaacc agtccagctc tttaccgcgg tcgagcataa tctgcaggct gccgatacca
    48421 ataaccagca gtgccagccc cacggcatca atccgccgcc gtttggtgcg ggtttcacgt
    48481 ccgcgcagag tttgcagtgt catcaacacc accgccacgc caatcggcac gttgatgaag
    48541 aatatccagc cccagtggta attatcgctg atataaccgc cgaggatcgg gccgcaaatt
    48601 ggcgcgacaa tcaccgtcat cgaccacaac gccagcgcga tcgagcgttt ggctggcggg
    48661 tagttattca gcaatagact ttgcgaaagc gggatcaacg gcccggcgac aatcccctga
    48721 atcacgcgga agaagatcag catattcagg ctgctggaga caccacacgc ccacgacgca
    48781 atagcaaagg cgatggtgga ccaaaggaac agtttcactt ccccgacgcg ctttgccagc
    48841 cagccggtaa gcgggatcga gatggcattc gccaccccga aagaagtgat tacccacgtt
    48901 ccctggctga gcgatgagcc cagattcccg gcgatagtgg ggatcgccac gttagcaatg
    48961 gtggagtcca gcacctgcat gaatgtcgcc agtgacagcg caatcgtcat aatgaccagt
    49021 tgcgcgcctt ccagcggttt ttgctgttgc atcacacgca cctctggatt agccagcgtt
    49081 agcttttacg atatcgtcga tcagtttatt gacaggtgcc aggctgattt cacgcgcggt
    49141 acttaccgcg accggtgttg aacgtacttt atttgccagt acctgaccgt cacggttagt
    49201 ggtattgacg ctcaccagtg tggacaaacc aatgcgcagc gggtattgct ccagctgttt
    49261 ctggtccagt tcgatacgca caggtagacg ctgaacgact ttgatccagt taccggtcgc
    49321 attttgcgcc ggaagcagtg agaatgcgct acctgtgccc atatccagac caaccacttt
    49381 accggtgtat ttcacatcat cgccgtaaat atccgtggtg atagtgaccg gctgaccgat
    49441 acgcatattg gcaatctgcg tctctttaaa gttggcatcc acccacatat tggtggctgg
    49501 aacgaccgcc atcagcggcg tcgttgggct aatttgcgcc ccaggctgta ccgcacggcg
    49561 ggagacataa ccggtcatcg gactgacaat acgggtacgc tccagcgcca gccaggcgtt
    49621 acgtacttcg gtggcagcct gttgcacggc aggctgatct tccagtttag tccccagaat
    49681 catcgcctga ttggcattgt attgttgaat agcgacgtcc agttgcgcct gggcactggt
    49741 gacggcgtcg cgggcgtgtt gcagctcttc gcgaccaatc aggttggcat tgcccagcgg
    49801 cacacggcgg ttgtagtcgc tttgtgcttt tgcgagggcg attttctgca cctcaatatt
    49861 cgcctgcaac tgtttgctgt taatcatcag ctggtgggtt tggcgaacgc tggaagccag
    49921 tgcagttttg gctttttcaa acgcctggcg agcatctgtc gggtcgagag tgaccagcac
    49981 gtcgccttct tttacaaaat cggtgttatc ggcccagact ttcgtcacgc tgccagacac
    50041 ctgagacata atttgaatct gattccctgc cacgtatgcg tcatcggttt cttcgaaatg
    50101 acgcagtacc aaaaaccaat aaatccctat cgctacggca ataattataa agagcaaggt
    50161 gagaaggagg agcagacgct tacgtttgcc gctcttcttt accggttgct gcggggtttg
    50221 agtctccgca tttgcgctca tattgttctc cacgatcttc ttaactcatc ggctgagccg
    50281 acctgcttgc caaaaaggcc agcatgttgc gatgctggcc ggtcattttt tcttttataa
    50341 atctggattt ttgagcgaga tgacgcgtta gctcatcgct tcgagaacca caccgtcttg
    50401 ttccatctgg tcgagacggg agagcaattt gcgggtgatt tgctcgagct gatctttttc
    50461 tgttgtgctg agcgcggacc agagttgatg caggcagtta tgctgcggtg gtaaaacctc
    50521 gcgcaaaaac tcgtgacctt tttccgttaa ttgcagatgc aggcagcggc gatcgttatc
    50581 gctttcacga cgttcgatcc aaccgcgttt ttccagttca tcggcaatac gcgtcgcgtt
    50641 ggtacgggat gatccaagag cacaacttaa ttcagaaggc tgaatactgt ggttttcctg
    50701 agactccagc gtaatcaacg ccataaacaa cgtctcgtta atcccctgag ccttcagcat
    50761 tttattgcgg ttctccagca gcttgctttg catgtgcatg caaagacgag tcagaaggat
    50821 ctcctgataa ggaaaatctt cgtggcggct ggcgcgaaat tttagcattt gttcaatggg
    50881 cgtaaacgaa ctatccattt gggtatgacc tcattaattt cagccgatat agtaacgaca
    50941 gtgacaaata aagtaaatgt attgttttag aaaaatgatt cttgtgggtt atataatcgc
    51001 catcactttc caggcgagcc cataggccag cgcactaagc agtgttggga tgataatgct
    51061 gcgtgtttta tagaaactgg cacccagtac cgcgaagccg accagcgtgg gcacgaaacg
    51121 gcgtgtatcg tgcatcactt ctggtgcggt agagacaacc agcagagcgc atatcgaggc
    51181 gatgccaatg gtgtcgagca aaatacctac cgcgccacgt ttggttgggc gggcattacc
    51241 cacacgcagg cgcagcggca aatagcggaa gcaataattc gccgcgccaa ccagtaaccc
    51301 aggcagcaga acctcatagc tcatcgggcg ctccttgcca gaatgcttgg attaacgcag
    51361 tgaggcagcc acagacaatg cctgccagaa tggcgacggg aatagaaaat agcgttacgc
    51421 ctgcaagggc accaactaac gctgcggtaa cgcaaagaga ttgtttgcgc tggaaagagg
    51481 cgagcaggaa actcataaag agtgccggaa gcataaaacc taatgctgct tcaacggcgg
    51541 gataaccttg cagcaagccg ctgccagaga atgcccctat taccgtacca aatacccacg
    51601 atgaccatga actgaaggca atgccgatca tccagttctc gctccagcgg cgattattgc
    51661 gtaccagttt tgctgttgcg gcggcaaaaa cctcatccgt caggccaaac gcccacaggg
    51721 cggttttcga tttttgcaga cgctgaataa tacggctacg cagtgacggg ccatacaaca
    51781 catggcgaac atccattgcc atgacggtca gtgcagcaac ccacaaacta ctcccggctg
    51841 ccagcatcgc ggtaatgacg aactggctcg cgcctgcata aatgatgcag gagaaaaaaa
    51901 cgctttcgag aggagagaat cccagacggg tcgcattcag accgaacgca aaggccaccg
    51961 gaatataact aataacaatc ggtaaactgt ctttgcatcc ttccatgaag gtcgccgaac
    52021 caggagcagg ctgtggagta gggctttcca tagagtgtac gcttaacgat tgttaatatt
    52081 ataaatagac tgaatgaata tcttaacctt atcagactga tgggcttctt aacaccctta
    52141 taagtgtaaa gccacgaaaa cggttgctga ttgcttaatt tgcggcttcc tgacgatgaa
    52201 aacctcgcca ccagaccagt aagttaacta gggcaatcgt cgcgccagcc agacaaacgc
    52261 cagcccaacc gccatgttgc caggctgagg ctgaaattag cgaaccggcg gcaccgccaa
    52321 taaagtagct ggtcatgtaa cctgcggtca ggcgattacg cgcatcaggg tgtattcgat
    52381 aaattaccgt ctggttagtg atatgcacgc cctgtacggt gagatccagc accaggatgc
    52441 cgataatcag cgccagtacg gaagtgtgac caaaccagat cgccagccat gaaagtaata
    52501 gcagcagcag accgaaagtt gtggtgtggt gcgatttacc cttatcggca aaaccgcccg
    52561 ccggacgagc gcccaacgct ccggcagctc ccgcaagtcc aaacagacca atgacaccat
    52621 cgctgtagtt aaaaggtgga gcggcaagca aaaaggccat tgaggtccag agaatgctga
    52681 agttggcaaa ggtcaggcag cccagcaatg cgcgggtgcg gaggatctta tcgctgataa
    52741 acatgctgaa aacggaacct aacaactgtg ggtagttgag gtgggtttct gatttcattt
    52801 gtggcagacc acgccataat gctagcgcca tcagtgccat taacaccgag gcaacccaaa
    52861 agacggtgcg ccagccgccg aggtttgcca gtaacccggc aacggttcgt gccagcaaga
    52921 tccccaacag cagaccgctc ataatggtgc caaccacttt gccgcgttta tccggtgaag
    52981 ccagcgtcgc tgccagcgga accagaattt gtgccacgac tgagaataaa ccggttaatg
    53041 ccgtaccgag gatcatcatc gccagcgact gactgctggc agtgattaac atgccgcctg
    53101 ccgccagtaa ggtcatcgag acaatcaggc ggcggcgttc aaacatatca ccgaggggaa
    53161 caagaaacag cagacctgct gcatagccca actgcgcggc ggtaacaata aagcctgccg
    53221 aactggcgga aagggaaaag ttacgcgcga tggtgtcgag caatggctgg gcgtaatagt
    53281 tactggctac cgcctgaccg gtggcgatag acatcagcac gatcagcgcc gggctaagct
    53341 catgattagg tttagtcatt atttttgttc atcaggtgat gattaagctg gaaatcaata
    53401 atagcggatg ttgatgaatg ggggcgaaag atagcttgtt gaaatgtgcg atgtggtttg
    53461 taggcatgat aagacgcgcc agcgtcgcat caggcattgt gcacagaacg gcggatgcgg
    53521 cgaaaacgcc ttatccgcca tacggaatga gtgtattttg taggcatgat aagacgcgcc
    53581 agcgtcgcat caggcattgt gcacagaatg gcggatgagg caaaaacgac ttatccgccc
    53641 gaataaaaat tacttctgcg ctgccagcgc ctcattcacc cagccatcga actgctgctg
    53701 gtgggctttg atccaaccat caacatggcc ctgaatatcg ccttctgagg ctttgccgtc
    53761 atgcataatg gcgttctggg cgttaatatc tgccactggc aactgcataa tggcaaaaag
    53821 tttcgctgct gccgggtttt tctcggccca ggctttgttg gcaacgatat gcatggtgct
    53881 gaccgggaag ccataattcg caccattcgg cagtttggta tcggcgtttt tatcgcccgg
    53941 cagtgcggag aacggcacct gcaaccagac cacatctttc cctggcttca gctcattact
    54001 cacccagtac ggcgtccagg tgtaataaaa caccggtttg ccctctttgt agcgactgat
    54061 ggtgtcggcc atcatcgctg cgtagttccc ctgattatgc gtcacggtgt tggtcagttc
    54121 atacgcggca agctggtggt tgatcgcacc ttcgcagccc cagcctgggt tacagccggt
    54181 taaatccgct ttaccgtcgc cgttggtatc gaacagtttg gcgatcttcg gatctttcag
    54241 ttgtgcgatg ttggtgattt tgtactggtc ggcggttttc ttatcgatca ggtaaccctg
    54301 tgccgcgccg ttaacgaata tcccttcaca ataaaatttc ttatcgccac cggcagcttc
    54361 gtacatgttg tcatgcagcg gcgtccagtt cacggcggtg aaggttgcat cgccggaagc
    54421 aagtgaggtg tagccaacgt tgtaatccac ttcgctgggt ttattgacgg tataacctaa
    54481 tttctccagt gcacgactga ccagcagcgt ctggaaggtt tcttcagtga tggtgctctg
    54541 aactggatta acagtaatgc ctttgcccgg cagatcggct gcaaaagttt gtgtagagat
    54601 aagcgtggca aacgctgtcg caaaaagtac gctatgtcgc attgttattc ctttttttgg
    54661 cagggtgata atgcccggcg ttaccgggca agtgcagagt tacttaatga atgggcgggt
    54721 cagcagaccg acagggccag tggtgtacca gcgacggttg ccgcgactgc gtgagtcgcg
    54781 cccaacggcc tgcgtcaggc ggtcgaggat aatggcgagg atcacaatcc cgacgccgcc
    54841 aacggtggca aggcccatat ccagacgacc gataccgcga agtaccatct gacccagccc
    54901 gccgacggca atcatcgagg cgatgaccac catagaaaga gccagcatca gcgtctggtt
    54961 aacgcccgcc ataatggtcg gcatcgccag cggtaactga actttgaaca gcatctggcg
    55021 cgggctggca ccgaatgagc gcgaggcttc aatcagatcc gccggaacct ggttaatccc
    55081 cagaatcgtc agacggataa tcggcggcag agcaaagatg atcgtcacca ctacgcccgg
    55141 cacgttaccg ataccaaaca gcatgacgat tggtaccaga taaacaaacg ctggcgtggt
    55201 ctgcatggca tcaagcagtg gacgaataat tttcgccgct cgcggacttc tcgccagcca
    55261 tatccccaac ggcaaaccga tgacgataca gaacagcagg gcggttaaca ccagcgccag
    55321 agtcaccatt gcctgcgacc aggcaccgat tgcgccgatg gcaatcagcg aaaccagcgt
    55381 cgccacgccc attccgaccc cggaaatctg ccaggcgatg agagcgaaaa cgataatcgc
    55441 cactggcgct ggcataccca gcagcaattg ctggaaactg ttgaggatat aatcaaccgg
    55501 aacgcgcacg ccctggaaga cgggacggaa atgggtaaca acccagtcga tcccttcagt
    55561 gacccaactg tcgagcggga tcagcgtttt atggaacgga tcgagaatat taaaatgctc
    55621 gacgtttggc gcaggcgtac tggtcagcca gtcagcacca ccgccgtcag tcggtgcagt
    55681 cgtcggtgta ccccaggcgt ctgcggattg cgcggcactg tccgccgctg gcgtggtatc
    55741 ccacggatta ttttgatcag ccattattta ccccctcacg atctaaagcg cgcagcagca
    55801 ttcctttcga aatgatgccg acatactgtt ggtcctcgtc gaccacgggc accgcacagg
    55861 gtgcctgtcc gacatgagag agcaactcgc taagaggcgt ttgtgcatcg actgctaacg
    55921 gcgcatcaat cagcgccgca tcaagacctt gctgctgcgt taacgcggtt ttaagcgaat
    55981 cgatggagac tgcgccgaca aacttattac cgcgttcgat aacgtagcca tattcgcgat
    56041 cttcatcctg caataatttc agtgccgaac gtgggccgaa gccaggggtt ttacgaatta
    56101 agccattcgg tgtccggcgg gcaatatctt tcgcactgaa tacctgacta atatcaacgc
    56161 cacggaagaa ggtacggaca taatcattcg ccggattatt gagaatttca tccggtgtgc
    56221 cgacctgtac cacttcacca ttttgcataa tggcaattcg gtcgccaata cgcatggctt
    56281 catcgagatc gtgggaaata aagacaatgg tgcgctgatg tttcgcctgt aattttacca
    56341 gctcatcctg catctcggtg cgaattaatg gatcgagcgc cgagaaggct tcgtccatta
    56401 ataaaatatc cggattaatc gctaacgcgc gggctaatcc cacacgttga cgcatcccgc
    56461 cagagagttc atccgggtag ctgtgggcat aattttccag cccgacctga cgcagtgcat
    56521 caagggcttt ttcccggcgt tcttcggcat taattccggc caattccata ccgaacgcag
    56581 tattgtccag cacggtcata tgcggcatta aggcaaagga ctggaagacc atcgcaatct
    56641 tttttctgcg cacctcacgg agtttggcat cagatatttt ggcaatatcc acaccatcaa
    56701 tcagcacttg cccgcgggtg ggttcaatca ggcgattgag aaggcgtacc attgtggatt
    56761 tacccgagcc ggataatccc atgatgacaa atatctcgcc ttcttcaatg gccagactgg
    56821 cgtcttttac gccaagcgat agcccagttt tttccagaat ttgttctttt gaaagtcctt
    56881 gttcgatata tttgaatgct cgctgtggat gctcaccaaa tattttataa aggtttttaa
    56941 tttctaattt aattgccatg caatagaaag attcctgtta tttgtttatg tcgatatatt
    57001 acccaatgga aatagttact tttttctacc ctaacatact gagaatctga ggcaacccct
    57061 gatggcaaat gtggcatgaa tattgatgcg agcaaatcgc cggaatttcc cgtgatataa
    57121 gggctgagag caaatcgaga aaagttgata ttttttgtca aagatagcga aaaaacgcct
    57181 gaatattgtg gtttcaggcg aataatgcaa cagagtatca gtgtagatca ccacaaaata
    57241 tttgcgtgat aaaaagctat ttggcgggaa ttatttccct gtcgcgggta gtgatatttt
    57301 tgaaaataac accctcagaa attccagtct tcatcttctg tttcaaccgc tttccccatc
    57361 acataagagg agcctgaacc ggaaaaaaaa tcgtgatttt catcggcatt cggcgaaagc
    57421 gcggcgagga ttgccggatt cacttccgcc atttctgcgg gaaataaagg ttcgtagccc
    57481 agattcatca aagccttatt ggcgttgtaa cagagaaacg ctttcacatc gtcagcccac
    57541 ggggtttcgg cgtacagctc atcggtgtat tgcagctcgt tgtcgtagag ttccagcaac
    57601 aaatcgaagg cgaaactctt caactcttca cgttgtccca gagatatctt ttccatgttt
    57661 ttctgatatt tatagcctat gtagtaaccg tggactgctt catcacggat aatcagacgg
    57721 atcaggtctg cggtattggt cagctttccg cggctggaaa aatacatcgg cagccagaaa
    57781 ccggaataga acaaaaaaga ttcaagaaac acactggcga ttttcttttt cagcggatca
    57841 tcaccgcgat aatgttgctg aataatctga gcttttcgct gcaacggtgc gttttcttca
    57901 ctccaggcgt aggcggcatc gacatctttg gtctggcaca gcgtcgagaa aatcgaactg
    57961 taagagcggg catgaaccgc ttccataaag ctgatattcg ataataccgc ttcttcatga
    58021 ggcgtgagtg catcgggcat cagcgaaggc gcgccgataa cattttgcag cgtgtcgagc
    58081 agcgtcaggc cagtaaaaac gcgcatcgtc agttgttgtt ctacgacagt taatgtctgc
    58141 caggcaggaa tatcgttcga cagcggcacc ttttctggta gccagaaatt gctggtcagg
    58201 cgattccaca cctccagatc tttatcgtca gatatcttgt tccagttgat ggcgctgata
    58261 cgtgagagtt tcatagatat tccttaaagt gcacaggaga cgcagccttc aatttcagtg
    58321 ccttccagcg ccatctgacg caggcgaatg taatagagcg ttttgatacc tttgcgccag
    58381 gcgtaaatct gcgctttgtt gatatcgcga gtggtggcgg tatcggggaa aaacagcgtc
    58441 agcgacaaac cctgatcgac atggcgagtc gcttccgcgt aggtgtcgat gatcttttct
    58501 gcgccgattt cgtaagcatc ctgatacagc gccagatttt cgttagtcat aaacggggca
    58561 gggtagtaaa cgcgtcctgt tttgccctct ttgcgtatct ctactttcgc cacaatcgga
    58621 tgaatactcg acgtagcatg gttgatataa gagatagaac cggttggcgg caccgcctga
    58681 agattctggt tgtaaatgcc gtagcgcatc acgtcgtcgc gcagttgcgc ccacatctca
    58741 cgggtaggta acgtaatacc gctgcgggca aacagttcgc caactttcgc cgttttcggc
    58801 tgccagttac cttgcagata ttggctaaaa tattcaccac tggcataacg tgactgtttg
    58861 aacccggcga aggtttcacc gcgttcgcgc gccagcaaca tcgaggtacg cagtgcgtgc
    58921 caggtgatgg catagaaata gagattggtg aaatccagtg cttccggcga accataagcg
    58981 atgccttctc gcgccagata gccgtgtaaa ttcatctgcc ccagtccgat ggcgtgtgag
    59041 gcggcatttc cggcttcgat ggacggcacg ctgcggatat gactcatatc tgacaccgcc
    59101 gttaaaccgc gcacggcagt ctctaccgtg cgggcaaaat cgggggaatc catggtgtgc
    59161 gcaatattca acgaacctaa attgcaggag atatcatggc ctgtgcgggc atagtcgaga
    59221 ttctcgtcat actctgaggc acagttgacc tgcaaaatct ctgagcagag attactcata
    59281 tttatgcgcc cggcgacagg gttagcacgg tttaccgtgt cttcatacat gatgtaggga
    59341 tagccggact caaactggat ttctgccagc cgctggaaga aatcacgggc gttgaggtat
    59401 tttttgcgga tgcgttcatc ggcaaccagt tcgtcatagt gttcgctgat ggcgatatcg
    59461 gcaaacggct tgccataaac tcgctcaacg tcatagggcg aaaacagcgc catctgcgca
    59521 ttctcttttg ccagatggaa agtgatatcc gggatcacca cgcccagcga cagtgtttta
    59581 atgcggattt tttcgtcggc attttcccgt tttgtatcga gaaaacgcag aatatcggga
    59641 tgatgagcat gtaaatagac tgcacctgcc ccctgacgcg cgccgagttg gttggcatag
    59701 gaaaatgcgt cttccagcat tttcatcacc ggaattacgc cagaagattg attttcaata
    59761 cgtttaattg gcgcgcccgc ttctcgcaga ttcgacagca aaaatgccac gccgccgccg
    59821 cgtttcgaca gttgcagtgc ggaatttacc gcccggccaa tcgactccat attgtcttca
    59881 atacgcagca gaaaacagga aaccagttcg ccgcgctgct gcttaccgca gttgaggaat
    59941 gtcggcgtgg ctggctgaaa gcgtcctgac agcatttcat cggtcagttg caacgccagc
    60001 gtcgcatcgc cttgtgccag cgtcagcgcc accatcgtga ctcgatcggc aaaatcttcc
    60061 agataacgtt taccgtcgaa tgtcttcaac gtatagctgg tgtaaaactt ccatgccccg
    60121 aggaatgtct ggaaacgaaa accgctggtg tgtgcgtggg caaacagcgt aatgacaaag
    60181 tcgcgagagt agcgattaag aacgctttca tcatagtaac cttcgttgac cagccagttc
    60241 aggcgctgct gctggctact gaaggtcaca ctgttcggac gcacatgcgt cgccataaag
    60301 gcgtcaacgg cctggcggtc tttatcgaac tgaatgcgac ctgcgctatc gtagaggtta
    60361 agcatcgcat tcagcgcgtg gtaatccatc gtttcctgcg tcaggcattc tgcggttgtc
    60421 gttgccaaaa ttcggttact cctttacgaa cgttttcgat atcgctttgc gtacccatga
    60481 gttcaaaacg gtacagccac ggcacgccgc atttccgggc aatcacatct ccggcgcggc
    60541 catacgcctc accaaagttg cgattaccag aagcaataac gccgcgaagc aacgcccggt
    60601 tgtgctcgtc gtttaaaaag cgaattacct atcgtggcac cgcgccagcc gtaccgccgc
    60661 cgccgtaaga gggcacgatc aggatgtaag gctcgtctac ctgaatccgt tcccgctcat
    60721 tgagcgggat gcgcaccgcg ggcagaccta aacgttcgat aaaacgctgc gtgttttcgg
    60781 agctgctgga gaagtagacg agctggctca tgcactggcc gcgtgtggcg ctggatgcag
    60841 acggttaatc atgtccggac ggaaaccaga ccagctaaga tcgccagcaa tcactaccgg
    60901 caactgacga aagccctgag cacgcaacgc ttccgctgct tcaggaacgc gatcgacatt
    60961 aatcatttca aaatcaaagc cccggttttc catcgcccgt ttggtggcgt ggcactgaac
    61021 gcaatcgtta cgagtgtaaa tagttatgcg catgattcgt atttccgttt aaaatgaaga
    61081 tacggcgcga tgatacgcgt cgggttgtct ctctgttgat acagagatac tagatgtagt
    61141 tgaaaaaaga ttcaaccaca caatatatag caaatagtga tcgaaattac cctagatatg
    61201 agcgtgacgg gggaggggga tttttgtgat tcaccaggca aaaagaaacc ccgaagacag
    61261 gcttcggggt taaagacgcg tatttattat catttttgca ctacgatttg cgcatgctta
    61321 acagtgcgcc gataaaaata cctaccgcag ctgctgtacc cacgctacac cagggtcttt
    61381 cacgaacaaa agaatccgcg cagccaacgg catcgcgtgc ggcttgctgg acgcgagtac
    61441 gaccatgcat tcgcgccctg gtttctttca gcaatgcctg agctttactg cgtgcggctt
    61501 cagcttcccc tttggcgtcg ctgccccagg acttcaatac agattcgagg ctgtcagcta
    61561 attgattgac atcattctgg atatcctgca cgccatcatc aacgtcgttg cggttcggtc
    61621 ggttaaacat atgatccccc attagaattc gatgtaattt tcagtgtaga ccataatttt
    61681 cttaactgag gttcatcgcg gctttatgga cttttctgaa agcgttgtga atgcgcaatc
    61741 aggtaaggta ggcccgcggt tgataataac gaggatcaaa tatgtattta cgaccagacg
    61801 aggtggcgcg cgtacttgaa aaagtcggtt ttactgtcga tgtggtaacc cagaaagctt
    61861 atggttaccg ccgtggggaa aattatgtct atgttaatcg cgaagcgcga atgggacgta
    61921 ccgcactggt aattcatcca acattaaaag aacgtagttc gacactggct gaacccgctt
    61981 ccgatattaa aacttgcgat cattatcaac aatttccgct ctatttagca ggcgagcgac
    62041 acgagcatta cggtatcccg catggcttca gttcgcgtgt tgcgcttgaa cgttatttga
    62101 atggtttatt tggcgaagcc agttaaagac gcgactggcg atgccagtcg cgaaaagaag
    62161 agtcaggctt ttacctgctg gtaatggctg actttaaaca gtcggcggca ataatcgagg
    62221 aagtagccat aaaccgcccc catcaacatc gaaacaacga tgtttgaact gaccgccgcc
    62281 ataatctgat gccagtctgc gcccaccact aacaagatcg ccacatacac cggtgactgg
    62341 aacgtcacat aagccaggat atcagccaga ttttttatcc agcccgacgg gctaactttg
    62401 cgtgccgcgc gcataaatag atcgcggtac ataccgtatg gccatgcaat taagatgttc
    62461 accggaatcg ctaccaatct ggaataaaaa gactgttcga agctcattcc ggagaggaaa
    62521 acttcaatac acatgttcac gacagaacag taaacaacca tcgcgaacgt atctgcaact
    62581 gcatgacgca agcgtgactg cggtgagaac atgctaatgc tccttaataa aagcgtaatg
    62641 ggatgttaat ggagatgaaa gcagtggatc tcattgccat ctgcattttc gcatagcgta
    62701 tatcgctggc tatgactatt caattagtga aaaattttta tttagtcagt ttttgtagat
    62761 aaaatattcc gtaacggctc gttttttgta cattgtggat ttttacatgc tgatatgatt
    62821 tattttcaat taaatcaatt agatatgacc ttgcctttaa tttcactaat gaaagtttgg
    62881 gggtgaggta acgctataag cgtagagaat tcggtgtatg tcgtgaccgt gtcatttaca
    62941 gcatttttga tgccagaaaa tgtacattaa cagcagaaag caccagtgag ggggg
//
LOCUS       DAJTYZ010000005        60487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000005 DAJTYZ010000000
VERSION     DAJTYZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.005"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..259
                     /locus_tag="OO596_000304"
     CDS             <1..259
                     /locus_tag="OO596_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HCR6716268.1"
                     /translation="GVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTTGGVY
                     PDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            372..1166
                     /gene="panB"
                     /locus_tag="OO596_000305"
     CDS             372..1166
                     /gene="panB"
                     /locus_tag="OO596_000305"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6716269.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1178..2029
                     /gene="panC"
                     /locus_tag="OO596_000306"
     CDS             1178..2029
                     /gene="panC"
                     /locus_tag="OO596_000306"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6716270.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2321..3217)
                     /gene="rpnC"
                     /locus_tag="OO596_000307"
     CDS             complement(2321..3217)
                     /gene="rpnC"
                     /locus_tag="OO596_000307"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6716271.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3491..3871
                     /gene="panD"
                     /locus_tag="OO596_000308"
     CDS             3491..3871
                     /gene="panD"
                     /locus_tag="OO596_000308"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6716272.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3875..5104)
                     /locus_tag="OO596_000309"
     CDS             complement(3875..5104)
                     /locus_tag="OO596_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6716273.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5168..5608)
                     /locus_tag="OO596_000310"
     CDS             complement(5168..5608)
                     /locus_tag="OO596_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6716274.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5713..6483)
                     /locus_tag="OO596_000311"
     CDS             complement(5713..6483)
                     /locus_tag="OO596_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6716275.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6480..7406)
                     /locus_tag="OO596_000312"
     CDS             complement(6480..7406)
                     /locus_tag="OO596_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6716276.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7515..8177
                     /gene="can"
                     /locus_tag="OO596_000313"
     CDS             7515..8177
                     /gene="can"
                     /locus_tag="OO596_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6716277.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8218..8754)
                     /gene="hpt"
                     /locus_tag="OO596_000314"
     CDS             complement(8218..8754)
                     /gene="hpt"
                     /locus_tag="OO596_000314"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6716278.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            8960..11350
                     /gene="gcd"
                     /locus_tag="OO596_000315"
     CDS             8960..11350
                     /gene="gcd"
                     /locus_tag="OO596_000315"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6716279.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VSAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11397..12947)
                     /gene="cueO"
                     /locus_tag="OO596_000316"
     CDS             complement(11397..12947)
                     /gene="cueO"
                     /locus_tag="OO596_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6716280.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13113..13460
                     /locus_tag="OO596_000317"
     CDS             13113..13460
                     /locus_tag="OO596_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6716281.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13566..14432
                     /gene="speE"
                     /locus_tag="OO596_000318"
     CDS             13566..14432
                     /gene="speE"
                     /locus_tag="OO596_000318"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6716282.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14448..15242
                     /gene="speD"
                     /locus_tag="OO596_000319"
     CDS             14448..15242
                     /gene="speD"
                     /locus_tag="OO596_000319"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6716283.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15280..15642)
                     /locus_tag="OO596_000320"
     CDS             complement(15280..15642)
                     /locus_tag="OO596_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6716284.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15817..18414)
                     /gene="acnB"
                     /locus_tag="OO596_000321"
     CDS             complement(15817..18414)
                     /gene="acnB"
                     /locus_tag="OO596_000321"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6716285.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18769..20526
                     /locus_tag="OO596_000322"
     CDS             18769..20526
                     /locus_tag="OO596_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR6716286.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20597..22021)
                     /gene="lpdA"
                     /locus_tag="OO596_000323"
     CDS             complement(20597..22021)
                     /gene="lpdA"
                     /locus_tag="OO596_000323"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6716287.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22346..24238)
                     /gene="aceF"
                     /locus_tag="OO596_000324"
     CDS             complement(22346..24238)
                     /gene="aceF"
                     /locus_tag="OO596_000324"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6716288.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24253..26916)
                     /gene="aceE"
                     /locus_tag="OO596_000325"
     CDS             complement(24253..26916)
                     /gene="aceE"
                     /locus_tag="OO596_000325"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6716289.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27077..27841)
                     /gene="pdhR"
                     /locus_tag="OO596_000326"
     CDS             complement(27077..27841)
                     /gene="pdhR"
                     /locus_tag="OO596_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6716290.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28382..29755
                     /gene="aroP"
                     /locus_tag="OO596_000327"
     CDS             28382..29755
                     /gene="aroP"
                     /locus_tag="OO596_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6716291.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29798..30652)
                     /gene="ampE"
                     /locus_tag="OO596_000328"
     CDS             complement(29798..30652)
                     /gene="ampE"
                     /locus_tag="OO596_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6716292.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30649..31200)
                     /gene="ampD"
                     /locus_tag="OO596_000329"
     CDS             complement(30649..31200)
                     /gene="ampD"
                     /locus_tag="OO596_000329"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6716293.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31288..32181
                     /gene="nadC"
                     /locus_tag="OO596_000330"
     CDS             31288..32181
                     /gene="nadC"
                     /locus_tag="OO596_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6716294.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32384..32824
                     /gene="ppdD"
                     /locus_tag="OO596_000331"
     CDS             32384..32824
                     /gene="ppdD"
                     /locus_tag="OO596_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6716295.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32834..34220
                     /gene="gspE"
                     /locus_tag="OO596_000332"
                     /pseudo
     CDS             32834..34220
                     /gene="gspE"
                     /locus_tag="OO596_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            34210..35412
                     /gene="hofC"
                     /locus_tag="OO596_000333"
                     /pseudo
     CDS             34210..35412
                     /gene="hofC"
                     /locus_tag="OO596_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35447..36490)
                     /gene="guaC"
                     /locus_tag="OO596_000334"
     CDS             complement(35447..36490)
                     /gene="guaC"
                     /locus_tag="OO596_000334"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6716296.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36646..36690)
                     /locus_tag="OO596_000335"
     CDS             complement(36646..36690)
                     /locus_tag="OO596_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716297.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36715..37335
                     /gene="coaE"
                     /locus_tag="OO596_000336"
     CDS             36715..37335
                     /gene="coaE"
                     /locus_tag="OO596_000336"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6716298.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37335..38078
                     /gene="zapD"
                     /locus_tag="OO596_000337"
     CDS             37335..38078
                     /gene="zapD"
                     /locus_tag="OO596_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6716299.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38088..38285
                     /gene="yacG"
                     /locus_tag="OO596_000338"
     CDS             38088..38285
                     /gene="yacG"
                     /locus_tag="OO596_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6716300.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38385..38774)
                     /gene="mutT"
                     /locus_tag="OO596_000339"
     CDS             complement(38385..38774)
                     /gene="mutT"
                     /locus_tag="OO596_000339"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6716301.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38834..41539)
                     /gene="secA"
                     /locus_tag="OO596_000340"
     CDS             complement(38834..41539)
                     /gene="secA"
                     /locus_tag="OO596_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6716302.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41601..42113)
                     /gene="secM"
                     /locus_tag="OO596_000341"
     CDS             complement(41601..42113)
                     /gene="secM"
                     /locus_tag="OO596_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6716303.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42344..43261)
                     /gene="lpxC"
                     /locus_tag="OO596_000342"
     CDS             complement(42344..43261)
                     /gene="lpxC"
                     /locus_tag="OO596_000342"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6716304.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43362..44513)
                     /gene="ftsZ"
                     /locus_tag="OO596_000343"
     CDS             complement(43362..44513)
                     /gene="ftsZ"
                     /locus_tag="OO596_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6716305.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44574..45836)
                     /gene="ftsA"
                     /locus_tag="OO596_000344"
     CDS             complement(44574..45836)
                     /gene="ftsA"
                     /locus_tag="OO596_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6716306.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45833..46663)
                     /gene="ftsQ"
                     /locus_tag="OO596_000345"
     CDS             complement(45833..46663)
                     /gene="ftsQ"
                     /locus_tag="OO596_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6716307.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46665..47585)
                     /gene="ddlB"
                     /locus_tag="OO596_000346"
     CDS             complement(46665..47585)
                     /gene="ddlB"
                     /locus_tag="OO596_000346"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6716308.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47578..49053)
                     /gene="murC"
                     /locus_tag="OO596_000347"
     CDS             complement(47578..49053)
                     /gene="murC"
                     /locus_tag="OO596_000347"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6716309.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49107..50174)
                     /gene="murG"
                     /locus_tag="OO596_000348"
     CDS             complement(49107..50174)
                     /gene="murG"
                     /locus_tag="OO596_000348"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6716310.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50171..51415)
                     /gene="ftsW"
                     /locus_tag="OO596_000349"
     CDS             complement(50171..51415)
                     /gene="ftsW"
                     /locus_tag="OO596_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6716311.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51415..52731)
                     /gene="murD"
                     /locus_tag="OO596_000350"
     CDS             complement(51415..52731)
                     /gene="murD"
                     /locus_tag="OO596_000350"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6716312.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52734..53816)
                     /gene="mraY"
                     /locus_tag="OO596_000351"
     CDS             complement(52734..53816)
                     /gene="mraY"
                     /locus_tag="OO596_000351"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6716313.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53810..55168)
                     /gene="murF"
                     /locus_tag="OO596_000352"
     CDS             complement(53810..55168)
                     /gene="murF"
                     /locus_tag="OO596_000352"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6716314.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55165..56658)
                     /gene="murE"
                     /locus_tag="OO596_000353"
     CDS             complement(55165..56658)
                     /gene="murE"
                     /locus_tag="OO596_000353"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6716315.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56645..58411)
                     /gene="ftsI"
                     /locus_tag="OO596_000354"
     CDS             complement(56645..58411)
                     /gene="ftsI"
                     /locus_tag="OO596_000354"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6716316.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58427..58792)
                     /gene="ftsL"
                     /locus_tag="OO596_000355"
     CDS             complement(58427..58792)
                     /gene="ftsL"
                     /locus_tag="OO596_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6716317.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58789..59730)
                     /gene="rsmH"
                     /locus_tag="OO596_000356"
     CDS             complement(58789..59730)
                     /gene="rsmH"
                     /locus_tag="OO596_000356"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6716318.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59732..60190)
                     /gene="mraZ"
                     /locus_tag="OO596_000357"
     CDS             complement(59732..60190)
                     /gene="mraZ"
                     /locus_tag="OO596_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6716319.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14452 a        16766 c        14895 g        14374 t
ORIGIN      
        1 aggagtcggt gtactcatag aaggtttagc aactagtaaa aatcctctaa tgacattgaa
       61 acctaatgat tcaaattctg tttataaaga ttacgacccg agaggcaaag acgatacaac
      121 aggaggagtt tacccggatc aagatacagg tataacatac cctctccatt tccaggccac
      181 gctacaacag gatggaacta taccaataga agctggtgag tttaaagcca ccagtacttt
      241 ccaggtaacc tacccttaat aagtccaccg caccgccatc atctggcggt gcggtaattg
      301 ataaatcatt gcccccacca atgtgactga taaaatgcca ggcaacacga catcatttat
      361 caggacacgt tatgaaaccg accaccattg cttcacttca gaaatgtaag caggataaaa
      421 agcgtttcgc gaccatcacc gcttacgact acagcttcgc gaaactattt gctgatgaag
      481 gacttaacgt catgctggta ggcgactcgc tgggcatgac ggttcagggt cacgactcca
      541 ccctgccggt gaccgttgcc gatatcgcct accacactgc ctccgtacgt cgtggtgcgc
      601 caaactgcct gctgttggct gacctgccgt ttatggcgta tgccacgccg gaacaagcct
      661 ttgaaaacgc cgcaacggtt atgcgtgccg gtgccaatat ggtcaaaatt gagggcggtg
      721 agtggctggt cgaaaccgta aaaatgctga ccgaacgtgc cgttcctgta tgtggtcact
      781 taggtttaac cccacagtca gtgaatattt tcggtggcta caaagttcag gggcgcggcg
      841 atgaagcggg cgatcaactg ctcagcgatg cattagcctt agaagccgct ggggcacagc
      901 tgctggtgct ggaatgcgtg ccgattgaac tggcaaaacg tattaccgaa gcactggcga
      961 tcccggttat tggcattggc gcaggcaacg tcactgacgg gcagatcctc gtgatgcacg
     1021 acgccttcgg cattaccggc ggtcacattc ctaaattcgc taaaaatttc ctcgccgaaa
     1081 cgggcgacat ccgcgcggct gtgcggcagt atatggctga agtggagtcc ggcgtttatc
     1141 cgggcgaaga acacagtttc cattaaggag tcacgttgtg ttaattatcg aaaccctgcc
     1201 gctgctgcgt cagcaaattc gccgcctgcg tatggaaggc aagcgcgtgg cgctggtgcc
     1261 tactatgggt aatctgcacg atggccatat gaagctggtc gacgaagcca aagcccgcgc
     1321 cgatgtggtc gtcgtcagta ttttcgttaa cccgatgcag ttcgaccgcc cggaagatct
     1381 ggcacgttat ccacggacct tgcaggagga ctgcgagaag ctaaacaaac gtaaagtgga
     1441 tttagttttc gccccttcgg taaaagagat ctacccgaac ggtactgaaa cccatactta
     1501 cgttgacgtt cccgggcttt cgaccatgct ggaaggcgcg agccgtccgg ggcattttcg
     1561 cggcgtttcg actatcgtca gcaagctgtt taacctggtc cagccggaca tcgcctgctt
     1621 cggtgaaaag gactttcagc aactggcgct aatccgcaaa atggttgccg atatgggctt
     1681 cgatattgaa attgtcggcg tcccgattat gcgcgccaaa gatggccttg cgctcagttc
     1741 ccgtaatggt tatctgacgg cggaacaacg caaaattgcg cccggtctgt acaaagtttt
     1801 aagttcaatt gccgacaaat tgcaggctgg cgagcgggat cttgatgaaa ttattgccat
     1861 tgcagggcaa gaactgaatg aaaaaggctt ccgcgccgat gatattcaga ttcgcgatgc
     1921 cgacacgttg ctggaagtct ctgaaaccag caaacgagct gtaattctgg tagccgcctg
     1981 gcttggcgat gcccgcctga tcgacaacaa aatcgtcgaa ctggcgtaat gcttttactc
     2041 taaataattc aagttgcagg aaggcggcaa gcgagtgaca aattcgtcag gaacgaattt
     2101 gcccagccgc aggctggcct tcggtgagag acatggatgt ctctcattaa tccccaggag
     2161 cttacataag taagtgactg gggttagcga acgcagacgc agcacatgca atttaaagta
     2221 tgacgagtat aactggcgct acggctgatg gcgccaatta ttaatttgcc ccacgtcatg
     2281 gcgatggcgt ctgtaagcaa ttattcataa cgcagacaac ttagttaatc agcctatcaa
     2341 tctcagcaag agataaattt gtcatctctg caacgtcttc ccgagacatc cctttactca
     2401 gaaaacgctg ggcgaattct tgcctttcct cctgtcttcc ctgctgaatc cccttctcaa
     2461 tccctttctc ttcaaaccac tgcgccagcg tcatcataga ctcccctccc gtttccctgt
     2521 ctctcagcac accataaaac aaatccgctt gttcagtatg accgcgttgc agcatatagt
     2581 tttgcatggc aactaactga cttccgctag tgtacccttc gtcaatcagc gtgaccagtt
     2641 gctccagcaa taacattaag tcgcgctggc gaatatgttt ttgcagtagt tcgagaatcg
     2701 caatccgccg atgttgcatg atttcgtcat ccggtgtgat ggtgatatcc accagcggga
     2761 aaggactgtt atagacgcgt cgtgccagct ccggcgagta aaatatatca aaccagcaca
     2821 ttgagagcgg ataaggtgtg gcctcgccct gataaaacag tatcggcacc accagcggca
     2881 gcttatcgtg atccgcctcc agatgccggt gcatggcagc aatagaataa cgcatcatgc
     2941 gaaagaccat tttcttatcc ggcttgcttt ggtgttcaat cacaacatgc agataaccgg
     3001 gattgccctg catttgcacg gaatagagca cgtccgtgct gtgtcctttc aggctctctt
     3061 caatgaaact ccccgactct aaatgaagcg tgttgaggtc acaaagttct cgtaattcca
     3121 ctggcaaatg tatctccaga aagtcgcgag ccgtctccgc gtgcattaaa aattgtttaa
     3181 acactgcatc atgcggtgtg gtactcggtg catccatctt ccctgccttc cgtgttgtct
     3241 tgcgtgaagg cagtatccag tcaaggcgtg ccatcgtcag ccacctcttt agtgtttacc
     3301 gagcagcgtt cagagaaatt tcaattggtt acagccgtta catggcacgc ttcggcacgg
     3361 tacgcaaact gctgatgtaa atgccgtcag atgagtagac actaaacaaa aatcgggcaa
     3421 tactgcgtga gaatttcctc aaagcaggca tcgcctgctt cgttaacgac agggtagaaa
     3481 ggtagaagtt atgattcgca cgatgctgca gggcaaactc caccgcgtga aagtgactca
     3541 tgcggacctg cactatgaag gttcttgcgc cattgaccag gattttcttg acgcagccgg
     3601 tattctcgaa aacgaagcca ttgatatctg gaatgtcacc aacggcaagc gtttctccac
     3661 ttatgccatc gcggcagaac gcggttcgag aattatttct gttaacggtg cggcggccca
     3721 ctgcgccagt gtcggcgata ttgtcatcat cgccagcttc gttaccatgc cagatgaaga
     3781 agctcgcacc tggcgaccca acgtcgccta ttttgaaggc gacaatgaaa tgaaacgtac
     3841 cgcgaaagcg attccggtac aggttgcttg attgttatcc ctgcggctgg ttactcacca
     3901 gccgcgacat cgtctccagc gaatccgttc ttaagatata aagtcgtttt agtaacaacg
     3961 gattatcccc cggttttact ttgcctttca tggttgtcac cgccaggtga aatccggcat
     4021 cgtttgctgc cttcacggct ttgtcattaa atccgccaaa cggatacgaa agataccaga
     4081 catgcggatt aaattgcgcc agagcgcggc gtgaacgtgc aaaatcaaac agaatattgt
     4141 gctcactacg gctcagtaat atgggtcggc gatagccatc cacccgatgc aaaaaatggg
     4201 tatgtgactg aaaatcaaaa acatcgcgaa tttcgttaag ctcagaaacg ctcataaatt
     4261 gcagcgattt tgggttccac ttctgcgggt gacgtttgat gcgcgaggta acaacaaacg
     4321 ccgtcgcctt catgccatat tgtttcaata caggatacgc atagcggctc accgacttga
     4381 ggccatcatc aaaggtaatc accaccgctc gcgcagggag attgatctta ttcttcacgt
     4441 aaccttccag ctgcgccatg ctcagtgtcg cgtatcccct gtcgcgcagc caggccattt
     4501 ggttattgaa agcgcgtacc gaggtggtcg tcgaagtatg gcgaaaacgg gtgttttctt
     4561 cgtcgcgcag aatatggtga taagttagca ccggaaggcc attatcgggt tgggcatcca
     4621 gcgcgctgat ataggccagt cgatcgccaa tacggacctg ataccaggtt tgatttaacc
     4681 tgtctttcag tttatgcaaa atcgggtagc gcaaattgtc cgccaatacc ccaaatggcg
     4741 cactgcccac actcggcgcg ttatagaccg gcgtatcttt ccaggtaact aagttctgat
     4801 tactcagcgg cttgttgagg tcacccaaac cgtcttcaac tttttgtcgc ccctgaaccg
     4861 gctcgagatg ccctttatcg ataaaccctt taccaaagcc aaaattaaat gcgtaataac
     4921 ttgcggcagt gggctccacg gcaatgattt gtccggcccg aatattcccc acggtcacca
     4981 tcttgtcgcc aatttgcgcc cagaccgcag cattttcgat ggtttgcata taacgggcag
     5041 gtaacgcggc actgacactt gcggtaaaca gcatcagcag gagaataaca gcttgtttgt
     5101 acatgaaaaa ataaccgagg gtgaaacaac gcttttattg tagcaaaagc gctataagaa
     5161 tcgtgggtta atactcatac aaattatgtt tgaggacgaa aggcggattt ttttgttgtt
     5221 ggtgccaaag actactcacc tccggcccac ccagttcgac aatacgctcg cgaaactctg
     5281 aactggtcat ggtttcacgg caagccatca tctgttcaat taacggtaac gtgacaccag
     5341 aaatcacttc gcaacgggag tgtttgtggc ttaataatga agccacgcgg tacggtggcg
     5401 ctccggctat atcggttaag aagatgacac cctcaccaga gtccgcttca tgcagagcat
     5461 cgcacatcat gcggctgagc atattagagc ttaacccacg ccagaaattc acggcccggc
     5521 actgaagaag cgccccatgt tttttctcca gcgcatccag tatctcttgc gcccggtcat
     5581 cgtgacaggt aattacccaa cctaacatcg ccttcctcct tagccgaaaa tcagtctacc
     5641 ctgaccattg ttgataaagg gtgatagccg tcaaaactcc tctcccccaa atccagagga
     5701 gaggaaatag ccttagctac gcaaaccacg tccacgttgg atcagcgacc aacagatcaa
     5761 ataaaacgcc acaataaaga ccaccagtac gccaaaggta gtgaccagcg gaacatcatt
     5821 gataccgagg aagccgtagc ggaaaccact gatcatataa acgattgggt tcaggtgcga
     5881 cagcccttgc cagaacggcg gcaacaaagt cagtgagtaa aagaccccac ccaaatacgt
     5941 gagtggcgtt aacacaaagg ttggcaccag gctgatgtca tcgaacgttt tggcaaacac
     6001 accgttcagc aaacccgcaa gggagaacaa caccgccgtg agcaccaggg ttaaggcaac
     6061 gaatacccac gaatgcacct gaaacggcac aaaaaacagt gaaattgccg tcaccagaat
     6121 gccaacaaac agaccacgcg ccacgccacc gccgacatat ccggcaataa tgacgtgagt
     6181 cggaaccggc gctaccagca gctcttcaat attacgctgg aacttggcac caaaaaatga
     6241 tgacgcaacg ttggcgtagg cattggtgat caccgacatc atgatcagcc ccggtacgat
     6301 gaactgcata tagctgaagc catgcatatc gccaatacgc gaaccaatca ggttaccgaa
     6361 gataataaag taaagggtca tggtgatgac tggcggcacc agcgtctgca cccagatacg
     6421 cataaagcga tggatctctt tcgcccagat gctttttagc gccacccagt aaagatgcat
     6481 catgcgcgat ctccttgttt ttcattaacc agtgaaacaa acagctcttc cagacggtta
     6541 gctttgttac gcatacttaa tacctgaatg ccctgctcac ttaactgcgt aaatacgctg
     6601 ttgatcccct gctcacgcag cacttcaact tccagcgtcg cggtatcgac cagtcgatac
     6661 tgatagccat cgagcttcgg taacggactt ttcggtgcga gatcgagaat aaaggtttcc
     6721 gatttcagct tcgccagcag cgccttcatc gaggtatttt ccaccagctc accgtgttga
     6781 ataatgccga tattgcggca cagcatttct gcttcttcca ggtagtgtgt ggtgagaatg
     6841 atggtggtgc ctttgtcgtt taaatccttc aaaaagcccc acattgagcg gcgaagttca
     6901 atatccacgc ctgcggtcgg ttcgtcgaga atcagtagtt taggttcatg cattaacgca
     6961 cgggcaatca ttaaacggcg cttcatcccg ccagataaca tacgcgcacg ttcgttgcgt
     7021 tttccccata gatcgagttg tttaagatat ttttcgctgc ggatgtacgc ctctttgcgc
     7081 tccacaccgt agtaccctgc ctgattcacc acaatttgct gcacggtttc aaacgggttg
     7141 aagttaaatt cctgcggcac cagtcccaac tgacgtttag cgttcacgac atctttctcg
     7201 agatcgtaac caaatacgct gacccgcccg gaggttttat ttaccagaga gctgataata
     7261 ccgatagtgg tcgatttccc ggccccgttc ggcccgagaa gcgcataaaa atcacccgct
     7321 tcgacctgca aatctattcc acgaagcgcc tgaacgccgc ctggataggt ttttttaagc
     7381 tgttgaagtt ccagtgcaat ggtcataaat ttttacttac cttacgttct tacactttat
     7441 ctgtggttta aatcgtcccg gagttgccct atattagcca aacgtaatta tttggttaca
     7501 ggtcgttaac ctccatgaaa gacatagata cactcatcag caacaatgca ctatggtcaa
     7561 aaatgctggt ggaagaggat cccgggtttt ttgagaaact ggcacaagcg caaaaaccgc
     7621 gctttctatg gattggatgt tccgacagtc gcgttcctgc agaacgttta accggtcttg
     7681 agccgggcga actctttgtt caccgtaatg ttgctaacct ggtcattcac accgacctga
     7741 actgcctttc cgtggttcag tatgcagtgg atgtactcga agttgaacac attattatct
     7801 gtggccacta cggttgcggc ggcgtacaag ccgccgttga aaacccggaa ctggggctta
     7861 tcaacaactg gctgctgcat atccgcgata tctggttcaa acatagctca ttgctcggcg
     7921 aaatgccgca agagcgccgt ctggatacct tgtgtgaact gaacgtcatg gaacaggttt
     7981 ataacctagg ccactccacc attatgcaat cagcgtggaa acgcgggcag aaagttacca
     8041 ttcacggctg ggcctacggc attcacgacg gcttgctgcg tgatctggat gtcacagcga
     8101 ccaaccgcga aacccttgag caacgttacc gtcacgggat ttccaacctc aagctgaaac
     8161 acgccaacca caaataaaaa tgccatgccg gatgcaacac atccggcaac ttcacactta
     8221 ctcgtcccgc agaatcactt tgccgatata cggcagatga cggtaacgct gtgcgtaatc
     8281 aatgccgtaa cccaccacaa actcatccgg gatcgagaaa ccgataaatt ctaccgggac
     8341 gttcacttca cgacgggacg gtttatccag cagcgtacaa atcgccagcg acttcggttc
     8401 gcgcaggctt aagatctcac gcactttcga cagtgtattc cccgagtcga tgatatcttc
     8461 aacaatcagc acgtccttgc cacggatatc ttcatccaga tctttgagga ttttcacatc
     8521 acgggtggtg gacatgccgc taccgtagct ggaggcggtc ataaagtcga cttcatgaga
     8581 tacctgaact tcacggcaca ggtccgccat aaacataaat gagccacgca gcagacccac
     8641 cagcaccata tcgctgccgc tgtctttgta acgctcagta atctgacgac ccagttcggc
     8701 gatacgcgct ttaatctccg cttcggggat cattacttct acagtatgtt tcatatctct
     8761 aaccatatga tttaaaaaca aatcacttcg cacgggcagg ttttggcacc gcaacaaagc
     8821 gcaagacacg cagtatacca gtaaaactat tcattcgcgg aatctgttaa taaagctcga
     8881 attgtgatga cggtcacaca tgttaaaacc tataattaat tgctactaaa atattaatga
     8941 attgaaatgg tgtctcttta tggcaattaa caatacaggc tcgcgacgat tactcgtcac
     9001 gctaacagcc ctttttgcag cgctttgcgg gctgtatcta ctcattggcg gaggctggct
     9061 ggtcgcgatt ggcggctcct ggtactaccc tatcgctggc cttgtgatgc tcggcgtcgc
     9121 ctggatgctg tggtgcagta aacgcgccgc gctttggcta tacgcagccc tgctgctcgg
     9181 caccatgatt tggggcgtct gggaagttgg tttcgacttc tgggcgctga ctccgcgcag
     9241 cgatattctg gtcttcttcg gcatctggct gatcctgccg tttgtctggc gtcgcctggt
     9301 cattcctgcc agcggcgcag ttgccgcact ggtggtggca ctgctgatta gcggtagtat
     9361 cctgacctgg gccggattta acgatccgca ggaaatcaac ggcaccttaa gcgccgatgc
     9421 cacacctgcg gaagctatct cacccgttgc cgatcaggac tggcctgcct atggtcgcaa
     9481 tcaggaaggt caacgctttt cacctctgaa acaaattaac gccgataacg tccataatct
     9541 gaaagaagcc tgggtgttcc gtactggcga tgtgaagcag ccgaacgatc cgggcgaaat
     9601 caccaatgaa gtgacgccga ttaaagtggg cgacaccctt tacctgtgta ccgctcacca
     9661 gcgcctgttt gcgctcgatg ccgccagcgg caaagagaaa tggcattacg atcctgagct
     9721 gaaaactaac gagtctttcc agcacgtaac ctgccgtggc gtctcttatc atgaagccaa
     9781 agcagaaacg gcttcgccgg aagtgatggc ggattgcccg cgtcgtatca ttcttccggt
     9841 caatgatggt cgactgattg cgattaacgc tgaaaacggc aaactgtgcg aaaccttcgc
     9901 caataaaggc gtgctcaatc tgcaaagcaa tatgccagac accaaaccgg gtctgtatga
     9961 accgacttcg ccaccgatta tcaccgataa aaccatcgtg atggccggtt cagttaccga
    10021 taacttctca acccgcgaaa cgtctggcgt gatccgtggt tttgatgtca acaccgggga
    10081 gctgctgtgg gcttttgatc ccggcgcgaa agatccgaac gcaatcccgt ctgacgaaca
    10141 cacctttacc tttaactcgc caaactcctg ggcaccagcg gcctatgacg cgaagctgga
    10201 tctggtctat ctgccgatgg gcgtgaccac gccggatatc tggggcggta accgcacacc
    10261 ggaacaggaa cgttatgcca gctcgattct ggcgctgaat gccactaccg ggaaactggc
    10321 gtggagctac cagaccgttc accacgacct gtgggacatg gatcttccgg cacagccgac
    10381 gctggcggac atcaccgtta atggtcagaa agtgccagtt atttacgctc cggcgaaaac
    10441 cggcaacatt tttgtgctcg atcgtcgcaa tggcgaactg gtggtttcgg ccccggaaaa
    10501 accggtgccg caaggtgctg cgaaaggcga ttacgttact ccgacccagc cgttctctga
    10561 actgagcttc cgtccgaaga aagatttgag cggtgcggat atgtggggag ccaccatgtt
    10621 tgaccaactg gtgtgccgcg tgatgttcca ccagatgcgc tatgaaggca ttttcacgcc
    10681 gccatctgaa cagggcacgc tggtcttccc gggtaacctg ggaatgtttg aatggggcgg
    10741 gatttccgtt gatccaaatc gtgaagtggc aattgccaac ccaatggcac tgccgtttgt
    10801 ttcgaaactg atcccacgcg gtccgggtaa cccgatggag cagccgaaag atgccaaagg
    10861 cacgggtacg gaatccggca ttcagccaca gtacggtgta ccgtatggtg tcacgctcaa
    10921 cccgttcctc tcaccgtttg gtctgccgtg taaacagcca gcatggggtt atatctcggc
    10981 gctggatctg aaaatcaatg aagtggtgtg gaagaaacgt attggtacgc cgcaagacag
    11041 tatgccgttc ccgatgcctg taccggtgcc gttcaatatg ggtatgccga tgctgggcgg
    11101 gccaatctcc acggcgggta acgtgctgtt tatcgctgct acggcagata actacctgcg
    11161 cgcttacaac atgagcaacg gtgaaaaact gtggcagggc cgtttaccag cgggtggtca
    11221 ggcgacgcca atgacctatg aagtgaatgg caagcagtat gtggtgatct ccgcaggcgg
    11281 tcacggttca tttggtacga agatgggcga ctatattgtg gcttatgccc tgccggatga
    11341 tgtgaaataa ccataatgcc cggagagatc cgggcatatt tccgaatacg gtctttttat
    11401 accgtaaacc ctaacatcat ccccgtatct tcatgctcca gcagatggca gtgcgccata
    11461 taagcatgtt ctttcggtgc gttgtgatta aacttcacca gcacttcact gacattgcct
    11521 tctaccttaa cggtatcttt ccagcccgcg cgatgagccg ctggcggttt gccattttct
    11581 gacaagatac ggaactgcat gccgtgaata tggaacggat gcagcatcat gtcgcccacg
    11641 ccagagataa cccaacgttc gtactgccct ttcgccgccg caaacatcgg cttattcata
    11701 tcaaacgcct gaccgttgat tttattggca tggtggaaat cgaacttccc gccgtggttc
    11761 atatgattca tattgccgtg ccccatatgg cccatcatct ggctgtgatc cattccggcc
    11821 atcgcctgat cgccatattt ctccattagc atctgcatcc ccatcatatc gagcatcggg
    11881 tccatagaaa gttgcagctt gcgtaccgtc agcccttcca gcgaaggtaa cgcaggcagg
    11941 ctacttaatg tgtcaggcaa agcaccggag gcactaatag caatcggctg aatccgcatt
    12001 accggatgcg gcttgtcaaa cggcgcaatc gccatcccca tctggctgac cggtagcgtc
    12061 accagatcaa agggtttgtt gtcgttaacc tccaccagca cttcaaaacg ctcgcccatc
    12121 agcaccggca gctcgctcac cttcaccggt tcaggtagca gaccaccgtc gctggcaatc
    12181 acatacagcg ggcgattgtc gctggtggcg aaattgagcg agcgggcgtt acagccattg
    12241 agcaaacgca ggcgcagcca gccacgcggg gcagcgtgtt gtgggtagat tgcaccgttg
    12301 gtcagcagcg tatcgccaaa ccagcccacg gcggcggtca tcacatccag ttgataatca
    12361 atctgcccat cagcgttaaa ttttttatcc tgaacaatca ccggaacatc atcgataccc
    12421 cactgttttg gcagcattaa tttcaggatc tcgtcatctt caattaccac cagcccagcc
    12481 aaccccatcg ccacctgtcg cccggtcttg ccatgttgat gtggatggaa ccagcaggta
    12541 gcggcaggtt gatcaacgtt caacgtcacc gtacgtttgc cgcctggcgg aataattccc
    12601 tgcgggccgc cgtcgacgtc acccggtact tccagcccgt gccagtgcaa cgtcgtctct
    12661 tccgccagtt ggttatagat atcaaccgtt accgctttgc cgcgctgtaa tttcaccgcc
    12721 ggccccagca gattgccgtt atagccccag gtagttgcag ttttctcgcc aaaggtagac
    12781 tgacctgcgc caatagttaa ctgaatgcga ttacgggcat cggtcgtgag caaatcaggg
    12841 atcggtaacg ttgggcgttc cgccgcaaag actgcgcggc tccacagcgg caaggctgaa
    12901 gccacaccca gcgcgacgga atattttaag aaatcacgac gttgcatagt tatttcctta
    12961 ttcttaagca ggcgaaacaa aatcaaacgt tgagcatagt ccttcccctt acgggaaggt
    13021 caagccgcag acacaattta atcgacaata atggtcgtcg cctcgctcgc agtgtgctaa
    13081 cgtttatctt ctttaagccc ctggtagaag caatgaagac gtttttcaga acagtgttat
    13141 tcggcagcct gatggccgtc tgcgcaaaca gttacgcgct cagcgagtct gaagccgaag
    13201 atatggccga tttaacggca gtttttgtct ttctgaagaa cgattgtggt taccagaact
    13261 tacctaacgg gcaaattcgt cgcgcactgg tctttttcgc ccagcaaaac cagtgggatc
    13321 tcagtaatta cgacaccttc gacatgaaag ccctcggtga agacagctac cgcgatctca
    13381 gcggcattgg cattcccgtc gctaaaaagt gcaaagccct ggcccgcgat tccttaagcc
    13441 tgcttgccta cgtcaaataa tccctgatac ctttttgtag aaatattgac cgtgcatccg
    13501 cggtcaatgt tagctattat gttgcgccct ttttttacgg gtgttaacaa aggaggtatc
    13561 aacccatggc cgaaaaaaaa cagtggcatg aaacgctaca cgaccagttt gggcagtact
    13621 ttgcggtaga taacgttctg tatcatgaaa agaccgatca ccaagatctg atcatttttg
    13681 agaacgctgc atttggtcgc gtaatggcgc tggatggcgt agtacaaacc accgagcgcg
    13741 acgagtttat ctatcatgag atgatgaccc atgttccgct actggcccac ggtcacgcga
    13801 aacatgtgct gattatcggc ggcggcgacg gtgccatgct gcgtgaagta acccgacata
    13861 aaaacgttga gtcaatcacg atggtggaaa tcgatgcggg tatcgtgtcg ttctgccgtc
    13921 agtatctgcc caaccataac gccggtagct acgacgatcc gcgctttaag ctggtgatcg
    13981 acgatggcgt caatttcgtt aatcaaacca gccagacctt tgatgtcatt atctccgact
    14041 gcaccgatcc tatcggtccc ggcgaaagtc tgttcacctc agcattttat gaaggctgca
    14101 aacgttgcct gaatcctggc ggtatcttcg tcgcacaaaa cggcgtctgc tttttacagc
    14161 aggaagaagc catcgacagc tatcgcaaac tcagccatta cttcagcgac gttggctttt
    14221 atcaggcggc gatcccgacc tattacggcg gtatcatgac ttttgcatgg gcgacagata
    14281 acgacgcctt acgccatctc tcaaccgaaa ttattcaggc gcgttttctc gcttctggcc
    14341 tgaaatgccg ttattacaat ccggcaatcc atacggcagc ttttgcctta cctcagtatc
    14401 tgcaagacgc actggcttca cagccgtcct aaggagaaga taagaaattg aaaaaactga
    14461 aactgcatgg ctttaataat ctgaccaaaa gtctgagttt ttgtatttac gatatctgct
    14521 acgccaaaac tgccgaagag cgcgacggtt atattgctta tatcgatgaa ctctataatg
    14581 ccaaccgtct gaccgaaatc ctgtcagaaa cctgttccat tatcggggcc aatattctta
    14641 acatcgcccg ccaggattac gaaccacagg gtgccagcgt cactattctg gtgagtgaag
    14701 aaccggttga cccgaaactc atcgacaaaa cagaacaccc cggcccactg ccagaaacgg
    14761 tcgttgccca tctcgataaa agtcatattt gcgtacatac ctacccggaa agtcatcctg
    14821 aaggcggttt atgtaccttc cgcgccgata ttgaagtctc tacctgcggc gtgatttctc
    14881 cgctgaaggc gctgaattac ctgatccacc agcttgagtc cgatatcgta accattgatt
    14941 atcgcgtgcg cggttttacc cgcgacatta acggtatgaa gcactttatc gaccatgaga
    15001 ttaattcgat tcagaacttt atgtctgacg atatgaaggc gctgtatgac atggtggatg
    15061 tgaacgtcta tcaggaaaat atcttccata ccaagatgtt gcttaaagag ttcgacctta
    15121 agcactacat gttccacacc aaaccggaag atttaaccga cagcgagcgc caggaaatta
    15181 ccgctgcgct gtggaaagaa atgcgcgaga tttattacgg gcgcaatatg ccagctgttt
    15241 aacggctctg gcggagctcc caggctccgc cagatttatt tacttctgct gcacgaaatt
    15301 gcggtaagcc gccacgacct gcagaaaatc ctcaacgccg catagcgaca ggctttcttc
    15361 gtcgtagtag ttcatccctt cttccatttc atcgccagcg aattccagtt gattggcgcg
    15421 aaccatcacc tcttcaccgt ccatccacag ggtgtattca tgccctgccc gttgccagga
    15481 ccgttcgcta cctttcagtg cgtgcgctgc ttgttccact tcatcaagca aagccaggtt
    15541 ttctttcacc tcttcattaa accagtgccc gaccacttca tgccccatgg acatacgcac
    15601 ctttaccact ccggtaatat cgcgcagaaa ttcgtaatcc atagtgtgtt cctctgctcc
    15661 cggcaatgcg ccgaaatcgc attgcgctat tgatgtaatt attgcagcct taccggagaa
    15721 aaacagcgga acggcgatgg tttagaaata aaaaatgccc gggaatctca ttcaccgggc
    15781 attgtgtcgt ttatgcgcag cgcgtgcgct tacttattac accgcagtct ggaaaatcac
    15841 cccatcggct ttttcggtgt actgagaaag ctggttgaag ttcagataac gataagtatc
    15901 aacggcagtt ttatctactt gcgcaacgta ggtctggtac tcttccggcg tcggcagttt
    15961 gccaatcagc gccgcaacag ccgccagttc tgcagaagcc aggaagacat tcgcgccagt
    16021 ccccagacgg ttcgggaagt tacgggtaga ggtggaaacc accgtcgcac cgtctgctac
    16081 acgcgcctgg ttacccatac acagagaaca gcccgggatc tcgatacgag ccccgctctt
    16141 accgaagacg ctgtagtagc cttcttcagt caactgtgcg gcgtccatgc gggttggcgg
    16201 tgccacccac aggcgggtcg gcaactggcc tttgtgcgca tccagcagtt tacccgcagc
    16261 acggaagtga ccgatgttag tcatgcaaga accgataaac acttcgtcga tcttctcgcc
    16321 ctgtaccgca gacagcggac gtgcgtcgtc cgggtcgttc ggtgcacaga ggattggctc
    16381 tttaatatcc gccagatcga tgtcgatcac tgccgcgtat tcagcatctg catcggcttc
    16441 cagcagctca ggattcgcca gccatttttc catgccctga atacgacgtt cgagggtacg
    16501 acgatcgcca taaccttccg cgatcatcca cttcagcagg acgatgttag agttcaggta
    16561 ttcgatgatc ggttctttgt tcagcttgat agtacaacca gcggcagaac gctcagcgga
    16621 cgcatcggtc agttcaaatg cctgctcaac tttcagatcc ggcagacctt caatttccag
    16681 aatgcgacca gagaagatgt ttttcttgcc tttcttctca acggtcagca gaccttgttt
    16741 gatcgcatac agcgggatcg cgtgtaccag atcgcgcagg gtgatgcccg gctgcatttt
    16801 gcctttgaag cgcaccagaa cggattccgg catatccagc ggcattacgc ctgttgcggc
    16861 tgcaaatgcc accagaccag aacccgccgg gaaagagata ccgatcggga aacgggtatg
    16921 ggagtcacca ccggtaccga cggtatccgg cagcagcata cggttcagcc aggagtggat
    16981 tacgccgtca cccggacgca gcgacacacc accacggttc ataatgaagt ccggcagcgt
    17041 gtggtgcgtg ttcacgtcaa ctggcttcgg atacgccgcg gtatggcaga aagactgcat
    17101 caccaggtca gccgagaagc ccaggcacgc caggtctttc agttcatcac gggtcatcgg
    17161 gccggtggtg tcctgtgaac ctacagaagt cattttcggt tcgcagtacg cgcccggacg
    17221 aatacctttc acaccgcagg cacgacctac cattttctga gccagcgaga agccgcgatc
    17281 gctctcagcg acatctttcg cctgacggaa cacatcactg tgcggcagac caagtgcttc
    17341 acgcgctttg gtggtcaggc cacgcccgat aatcagcgga atacggccac cagcacgcac
    17401 ttcatcaatc agcacgtcgg ttttcagttc gaaggtcgcc agcagttcgc cggtttcgtg
    17461 gttacgcact tcacctttgt acgggtaaac gtcaatcacg tcgcccatgt tcaggttaga
    17521 gacgtcgact tcgattggca gtgcacccgc gtcttccatc gtgttaaaga agatgggtgc
    17581 aattttaccg ccgaggcaca aaccaccgcc gcgtttgttc ggcacatgtg gaatatcatc
    17641 gcccataaac cacagaacgg agttcgtggc ggatttacgc gatgaacccg tacccacaac
    17701 gtcaccgacg tacgccagcg ggaaaccttt ctgttgcaga gcttcgattt gcttgatcgg
    17761 gccaacaaca cctggctggt ctggctcaat accttcacgg gcgtttttca gcatcgccag
    17821 cgcgtgcagt gggatatccg ggcgcgacca cgcatccggt gccggagaaa ggtcatcagt
    17881 gttggtttcg ccagtgactt tgaagacggt aacggtcagt ttttcagcca gcgccgggcg
    17941 attcaggaac cattcggcat ccgcccagga ctgcattacc tgcttcgcat attcgttgcc
    18001 tgctttggct ttctcttcta cgtcatagaa gttatcgaac atcagcagtg tgtgagaaag
    18061 tgctttggca gcgatcggtg ccagtttggc atcatccagc gcgtcgatca gcggatgaat
    18121 gttgtaacca ccctgcatgg tacccagcag ttcgatggct ttttccggag tcagcagagg
    18181 ggatttggct tcgcctttag cgatagcagc caggaagcct gctttgacat aggcggcttc
    18241 atcgacgcca gggggaacac ggttggttaa tagatctaac aggaattctt cttcgcccgc
    18301 gggcgggttt ttcagcagct ctacaagtgc ggccatttgg tttgcatcca ggggtttggg
    18361 cgcaatcccc tcagcggcac gctcagctac gtgcttacgg tattcttcta gcacgacggt
    18421 tctcctcgct ctcattgtca tagtgcggca ggcgattctc ttcacgctcc tgtgagacag
    18481 cagtttgtag ggtaaatgcc cagtaccgca acgggcagaa tagcaggatt ttgacgacgt
    18541 gttaatctgt ttacaaaaaa gcaacataaa aaagtggctg aatcgttaag gatggtctag
    18601 agattgtttc cctccgtaaa aacttcgtta attcgcatgg tgataatcac ctttctcaac
    18661 gaatcccaag attagtcaaa atttaaacta ccgcctcttt atactcggat taacagcacc
    18721 tgcgggtggc agttcgccga ccattgcgat ttccttgaga tccgaattat gaaaatgact
    18781 ttgccgttta aaccccatgt gctggcacta atttgcagtg ccgggctttg tgccgcctct
    18841 gccgggctat atataaaaag ccgcacagtg gaagcgcctg tggaaacgca atcgacacaa
    18901 cagactgcgc ctgacatcac cgcagttacg cttcctgcaa cggtttccgc accgcccgta
    18961 acgcccgccg tcgtcaaatc cgcattcagc actgcacaaa tcgatcaatg ggttgcgcct
    19021 gtcgcgctgt accccgattc tctgctttca caagtgttaa tggcatcaac ctatccggca
    19081 aacgttgctc aagcagtgca atggtcgcac gataatccac ttaaacaagg cgatgctgct
    19141 attcaggcgg tatctgacca gccgtgggac gccagcgtta aatcactggt ggcctttcca
    19201 caattgatgg cattgatggg cgaaaacccg caatgggtgc aaaacctggg cgatgctttt
    19261 ctggcgcagc cgcaggacgt gatggactcg gtacagcgat tgcggcaact ggcgcaacaa
    19321 accggctcgc tgaagtcatc aaccgaacag aaagttatta ccacaacgaa gaaagctgta
    19381 ccggtaaaac agacagtcac ggcacccgtc ataccatcca ataccgtttt aactgccaac
    19441 cccgtcatta cagagccggc aacaaccgtc atttccattg agcccgccaa tcctgatgtg
    19501 gtctatattc ccaactacaa cccaaccgtg gtttacggga actgggccaa tactgcgtat
    19561 ccgccggttt atctgccacc accagccgga gaaccgtttg ttgacagctt tgtgcgcgga
    19621 ttcggctata gcatgggcgt tgctaccacg tacgcactat ttagcagcat cgactgggat
    19681 gacgacgatc atgaccatca tcatcatgac gatgatgatt atcatcacca cgatggcggt
    19741 catcgtgacg gtaatggctg gcaacacaac ggcgacaaca tcaatatcga cgtcaacaat
    19801 ttcaaccgta tcaccggtga gcatcttact gataagaata tggcatggcg gcacaatcca
    19861 aactaccgta atggtgtgcc ctatcatgat caggatatgg caaagcggtt tcatcaaacc
    19921 gatgtcaacg gcggaatgag tgccacgcag ctacctgctc caacacgcga cagccagcgt
    19981 caggcggcag caagtcagtt tcagcaacga acacacgccg cccccgtcat tacacgagat
    20041 acccaacgtc aggcagcggc acagcggttt aatgaagctg aacactatgg gagctatgac
    20101 gacttccgcg acttcagccg tcgccaacca ctgacccagc aacaaaagga cgccgctcgt
    20161 cagcgttatc aatcggcctc tcctgagcag cgccaggcgg ttcgcgagaa aatgcagact
    20221 aacccacaga accagcagcg aagagaggca gcgcgtgagc gtattcagtc tgcaactccc
    20281 gaacagcgcc aggtgtttaa ggaaaaagta cagcagcgcc cactgaacca acagcaacgt
    20341 gataacgccc gccagcgtat acaatcggca tcacctgaac aacgtcaggt ttttcgggaa
    20401 aaagttcagg agagccgccc acaacgtcta aacgacagta accatactgt caggctgaat
    20461 aacgagcaac ggtcagcagt acgcgaacgt ctctctgagc gcggagcaag gcgacaggaa
    20521 aggtaaattg cagacgtaaa aaaagcggcg tggttagccg cttttttaat tgccggatgt
    20581 tccggcaaac gaaaaattac ttcttcttcg ctttcgggtt cggcaggtcg gtaatgctac
    20641 cttcgaacac ttctgccgcc aggcccacag actcgtgcag agtcgggtgc gcgtggatgg
    20701 tcagtgcgat gtcttcagca tcacaaccca tttcgattgc cagaccgatt tcacccagca
    20761 gctcaccgcc gttagtaccg acaatcgcac caccgatcac acggtgagat tctttgtcga
    20821 aaatcagctt ggtcataccg tctgcgcagt cggaagcgat agcacgacca gaagcagccc
    20881 acgggaaggt ggcggtttca tagctgatgc ctttctcttt cgcttctttc tcagtcagac
    20941 ctacccatgc aacttctggt tcggtatagg cgatagacgg gataactttc ggatcgaagt
    21001 agtgtttttt accggcgata acttcagcgg caacgtgacc ttcgtgaaca cctttgtgtg
    21061 ccagcatcgg ctgaccaacg atatcgccga tagcaaagat gtgcggtacg ttggtacgca
    21121 gctgtttgtc aacgcggatg aaaccacggt cgtccacttc cacgccagct ttgcctgcgt
    21181 cgaggttttt accgttcggc acacgaccaa tcgctaccag cacggcgtcg taacgctgcg
    21241 gttcagcggg tgcttttttg ccttccatcg tcacataaat accgtcttct ttcgcttcaa
    21301 cggcggtaac tttggtttcc agcatcaggt tgaatttctt gctgatacgc ttggtgaaga
    21361 ctttaacgat gtctttgtca gctgccggga taacctggtc gaacatttca accacgtcaa
    21421 tctgtgaacc cagcgcgtgg tatacggtgc ccatttccag accgatgata ccgccaccca
    21481 ttaccagcag gcgttctggt acttctttca gttccagcgc gtcagtggag tcccagatac
    21541 gcggatcttc atgcggaata aacggcagtt ggatcgggcg agaacccgct gcaatgatcg
    21601 cattgtcgaa gttgatcacg gttttgccgt tctcaccttc aacttccagg gtgttagccc
    21661 cggtgaattt acccagaccg ttgaccactt tgactttgcg gcctttcgcc ataccagcca
    21721 gaccaccggt cagctgattg atcactttct ctttccaagt acgaatcttg tcaatatcgg
    21781 ttttcggttc gccgaagacg ataccgtgtt cagccagcgc tttggcttct tcgataactt
    21841 ttgctacgtg cagcagtgct ttagaaggga tacagccgac gttcaggcaa acaccgccaa
    21901 gggtgttgta acgttctacg attacggttt ccagacctaa atcagcgcaa cggaaggcag
    21961 cggagtaacc tgcggggcct gccccaagta ccacgacctg agttttgatt tcagtactca
    22021 tcatgacctc tatatattta tctccggcgg tcatacccgt cgtctttcag gccgcagcgg
    22081 cgttagcttc acgccctcac cccagtcact tacttatgta agctcctggg gattctggcg
    22141 cttgctacct tgctgcaccc agaaatccat agggtatgtg aagtaatacc ctaaccacca
    22201 ccgggtcgtt ctatccgtcg gtattttaca aaattgttaa caatttttaa acaacaaacg
    22261 gcaaccgatt tgtctattcg ctaataacct caatacattc atgagattac cagaaaaaag
    22321 ccggccgttg ggccggctct tttacttaca tcaccagacg gcgaatgtca gacagcgtgt
    22381 tgttaatgat ggtaatgaaa cgggcaccat cagcaccgtc gatcacgcgg tggtcgaagg
    22441 agagagaaat cggcagcatc agacgcggca cgaactcttt accattccac accggctcca
    22501 tcgcggactt ggaaacgccg aggatagcca cttccggcgc gttcacaatc ggcgcgaagt
    22561 gggtagtacc caggccgccg atgctggaga tggtgaagca accgccctgc atttcgcccg
    22621 cagtcagctt accgtcacgc gctttcttag aaatagtcat cagctcgcga gacagctcga
    22681 tgatgccttt cttgttgacg tctttgaata ccggaacaac cagaccgttc ggggtatcca
    22741 ccgccacacc gatgttgatg tatttcttca gggtcagacg ctgaccgtct tccgacagcg
    22801 aactattgaa gcgaggcatc tgctcaagag ctgcagcaac ggctttcatg atgaagacaa
    22861 ccggggtgag cttcacatcc agcttacgtt tcgccgcttc ttcgttctgc tgtttacgga
    22921 acgcttccag ctcggtgata tcggttttgt cgaagtgggt aacatgcggg atcatcaccc
    22981 agttacggct caggttagca ccagaaattt tctggatacg gcccagttcc acttcttcga
    23041 tttcgccaaa cttgctgaag tccaccttcg gccacggcag catgccaggg ataccaccgc
    23101 cagtcgccgc cggagctgct tctgcacgtt tgatagcttc tttcacgtaa gcctgaacgt
    23161 cttcgcgcag gatacgacct ttacgaccag tgcccttcac tttcgccagg ttaacgccaa
    23221 actcgcgtgc cagacggcgg atcagcggag tcgcgtgaac gtaagcgtcg ttttcagcaa
    23281 attcagattt gccttcggct ttcgcagccg gagctgctgc cggggcttca gctttcgctg
    23341 ccggagccgg cgctgccgct tcctgtttcg caggagctgc cgcaggcgct gcgccttcaa
    23401 cttcgaagat cataatcagc gagccagttt tcactttatc gccaacgttg actttcagtt
    23461 ccttcacgac gcctgcaaac ggagccggga cctccataga agctttatcg ccttctacgg
    23521 tgataagcga ttgttcagcg gcaactttgt cgcccacttt caccatcact tcggtcactt
    23581 caacttcgtc accgccgata tccggaacgt taacttcttt cacgccagcc gctgatgcag
    23641 gggccgctgc cggagctgct tcctgtttag ccgccggagc tgccgcgcct gcttcacccg
    23701 cgacttcgaa gaccataatc agcgagccgg tagacacttt gtcacccacg ttcactttga
    23761 tctctttcac ggtgccagca aacggagccg gaacttccat agaagcttta tcgccttcta
    23821 cggtgatcag cgactgttca gcttcaactt tatcgccaac tttcaccagg atttcggtca
    23881 cttcaacttc gtcgctgccg atatccggaa cgttaacgtc ttttgccgcc gcagccgcgg
    23941 gtgctgctgc cggagctgct tctttcttct cttctgcctg agcaggtgca gcgtctgctg
    24001 caccgtcggc ggaatcgaaa atcataatca gtgcgccggt ctgggtttta tcgccaacag
    24061 agactttgat ctctttaacg atacccgcct gcggagacgg aacttccata gaggctttgt
    24121 cgccttctac ggtgatcagc gactgttcgg cttcaacttt gtcgcccact ttgaccagga
    24181 tctcggtgat ttcaacttca tcagccccga tgtccggtac tttgatttcg atagccatta
    24241 ttcttttacc tcttacgcca gacgcgggtt aactttatct gcatcgatgt tgaatttggc
    24301 gattgcgtca gcaaccactt tcttatcgat ttcgccacgt ttagccagtt cgcccagcgc
    24361 cgcaaccacg acataagaag catcaacttc gaagtggtga cgcaggttct cacggctgtc
    24421 ggaacgaccg aagccatcag tacccagtac gcggtagtcg tcagccggta cgtaagtacg
    24481 gacctgctca gcgaacagtt tcatatagtc ggtagatgcc actgccggag cgtcgttcat
    24541 cacctgagcg atatacggaa cgcgcggagt ttccagcgga tgaagcatgt tccagcgttc
    24601 acaatcctga ccatcacgcg ccagctcggt gaaggaggtc acgctataaa cgtcagaacc
    24661 tacgccgtaa tctttcgcca ggatctctgc cgcttcacgg acgtgacgca ggatagaacc
    24721 ggagcccagc agctgaactt tacctttgct accttcaatg gtttcgagtt tgtagatacc
    24781 tttacggata ccttcctcag caccttccgg cattgccggc atgtggtagt tttcgttcag
    24841 cgtagtgatg tagtagtaaa cgttctcttg tttttcaccg tacatacgct ccagaccgtc
    24901 atgcatgatg acagcaactt cgtaagcgta agccgggtcg taagagatac agttcgggat
    24961 agtcagcgac tgaatgtggc tgtgaccatc ttcgtgctgc agaccttcac cgttcagggt
    25021 ggtacgaccg gaagtaccgc cgatcaggaa gccacgcgct tgctggtcgc cagccgccca
    25081 gcacagatcg ccgatacgct ggaagccgaa catcgagtaa tagatgtaga acggaatcat
    25141 cggcagattg ttggtgctgt aagaggtcgc cgctgccagc caggaacacc ctgcgcccag
    25201 ctcgttgatc ccttcctgca gaatctgacc tttctcgtct tctttatagt aagcaacctg
    25261 ctcgcggtcc tgcgggatgt actgctgacc gttcgggctg taaataccaa tctgacggaa
    25321 cagaccttcc ataccgaaag tacgcgcttc gtcggcgatg atcggtacca gacgatcttt
    25381 gatcgacttg ttcttcagca ttacgttcag ggcacgaacg aaagcgatag tggtagagat
    25441 ctctttgctc tgctcttcca gcagcgcgcc gaagtcttgc aggctcggca gctcaagctt
    25501 ctcggtgaag ttcggctgac ggcttggcag ataaccgtgc agtttctgac gctgtgcgtg
    25561 cagataggta tgctcttcag aaccttccgg gaaggtgatg tacggcagtt tttcgatatc
    25621 tgcatcagac accggcacat tgaaacggtc gcggatgtga cgcacgccgt ccatgttcat
    25681 tttcttaacc tggtgcgcga tgtttttacc ttcagccgcg tcgcccatgc cgtaaccttt
    25741 aatggtatga gcaaggatta ctgtcgcttt gcctttggtt tcctgcgctt tcttgaatgc
    25801 agcgtagatt ttcttcggat cgtgaccacc acggttcagt gcccagatct gctcgtcagt
    25861 ccagtctgca accagtgctg cggtttcagg atatttaccg aagaagtgtt cacgaacgta
    25921 cgcaccatct ttcgatttga aggtctggta gtcgccgtca acggtttcgt tcatcagctg
    25981 gatcagttta ccgctggtat ctttacgcag cagttcatcc caacggctac cccacatcac
    26041 tttgataacg ttccagccag caccttcgaa gatgccttcc agttcgttga tgatcttgcc
    26101 gttaccggtg accgggccgt caagacgctg caggttacag ttgataacga agaccaggtt
    26161 atccagtttt tcacgggtag cgatggtgat cgcacctttg gattccggtt cgtccatttc
    26221 accgtcgccg aggaacgcgt aaacggtctg tttagaggta tctttcaggc cacggtgttc
    26281 cagatatttc aggaatttag cctggtaaat agcaccaatc ggccccagac ccatagatac
    26341 ggtcgggaac tgccagaatt ccggcatcag tttcgggtgc ggataggaag agaggccatt
    26401 gccgtgaact tcctgacgga agttatccag ctgctcttga gtcagacgac cttccaggaa
    26461 agcacgagcg tacacgcccg gggagatgtg gccctggaag taaaccaggt cgccgccatc
    26521 ctgctcgttg cgtgcacgga agaagtggtt aaagcacaca tcataaatgg ttgcggaaga
    26581 ctggaaggac gccatatggc cgcccagttc gaggtctttt ttcgacgcac gcagcaccgt
    26641 catgatggcg ttccagcgga tagctgaacg aatacggcgt tccagttcca gattacccgg
    26701 atactccggt tgttcttcaa cggggatggt gttgatgtag ttgctgatac ctgtgcctgc
    26761 ggctacgttt acaccgcctt tgcgggcttc agcaagcagt tggtcgatca gatactgagc
    26821 acgctcaaca ccttcttcac ggatgaccga ttcgatcgcc tggagccagt cgcgagtttc
    26881 gatcggatcc acgtcatttg ggaaacgttc tgacatgggt tattccttat ctatctaata
    26941 acgttgagtt ttctggaacc tgtcccattg aactctcgcc ggaaagctca ataagacagg
    27001 ttctacgttt agttgccgcg cttttatata cgcttgattt acaacatctt ctggataatt
    27061 tttaccagaa aaatcactaa ttctttcgtt gctccagacg acgcagagaa cgctcacggc
    27121 ggctctcttc acgacttctg tcgagcaaaa tttcttcgat aaaggccaga tggcgatgcg
    27181 atgcttcgcg cgcttcttcc ggcttaccgg ccataatcgc ttcaaatatg cgggtgcggt
    27241 gactactcac cagcggcagc atctcgcgac gcgaatagag caattcgaag ttctggcgga
    27301 cattctgggc caacatcggc tccatacacc ttagcagatg aagcagaacc acattgtggg
    27361 ccgcttcggt gacggcaatc tgatactgga gtacggcgtt tgattccgcg tccagatcgc
    27421 cagactgctg cgccagctct atggcgtggt ggagttcacg gatgcgttcc ttgtcttcat
    27481 cggtactacg cagcgcggcg taataagcgg cgataccttc cagggcgtgt cgtgtttcga
    27541 gcaagtcata ctgtgattca ggatggtcgg agagcagctc caccagcgga tcgctgaagc
    27601 tttgccatag gctgctctgg acaaaagtgc cgccaccctg gcgacgaagc aacaagccct
    27661 tcgcttcgag acgttgaatc gcctcacgca aggagggacg ggagacgtcg aactgttttg
    27721 ccagttcgcg ttccggtggg agtttttcgc ccgggcggag agtgccttcg agaatcaaaa
    27781 actccagttg ctgctcaatc acatcggaga gttttggttg gcggattttg ctgtaggcca
    27841 tgagttcctg tcttaagcca cttgccgaag tcaattggtc ttaccaattt catgtctgtg
    27901 acgctaaagt aacaaagtat tcaccttatg tccatacagg ttttgattga aatcatgaaa
    27961 ctgtgcacat tttaacaact tgacatatat aacgtttcaa aattgtaact atgcacaaat
    28021 gtagacttta cgtaggaaag ggatttttta acgattatta aactataaaa atccgaattg
    28081 aaccgattca cttaccaatt ttttgatttt taagtcaata aaaacgaatt taaattcatt
    28141 ctgcatattg agaggggttg aggctgagct ttacaaacgg tttcttttta agcaactcat
    28201 cttcaaccat gcataaagcg ggtgcattcg ctgccgcata ccattattct tgatctgacg
    28261 gaagtctttt tgtaacaatt caaacttctt tgatgtaaaa aaattaatac aacaaacgga
    28321 attgcaaact tacacacgca tcactgcgta gatcaaaaaa acaatcaccg cacgaggttt
    28381 catgatggaa ggtcaacagc acggcgagca gctaaagcgc ggccttaaaa accgccatat
    28441 tcagcttatc gcgctgggtg gcgcgatagg gacagggcta ttcctgggta gcgcctccgt
    28501 aatacagtcc gcagggccag ggattatcct gggttacgcc attgctggtt ttatcgcctt
    28561 tctgatcatg cgtcagctgg gtgaaatggt ggtcgaagaa cctgtcgcag gctcctttag
    28621 ccactttgct tataaatact ggggcagttt tgccggtttc gcctctggct ggaactactg
    28681 ggtactgtac gttttagttg ccatggcaga gctgactgcc gtgggtaaat acattcagtt
    28741 ctggtatccg gaaatcccaa cctgggtttc tgccgccgta ttctttgtgg tgattaacgc
    28801 catcaacctg accaacgtta aagtgtttgg cgagatggag ttctggtttg ccattatcaa
    28861 agttattgcg atagtagcga tgatcatctt cggcggctgg ctgctgttca gtggcaacgg
    28921 cggcccgcag gcgagcgtta gcaacctgtg ggatcaaggt ggtttcctgc cgcacggctt
    28981 caccggactg gtgatgatga tggcgattat catgttctcg ttcggtggtc tggaactggt
    29041 ggggatcacc gcagcagaag ctgataaccc ggagcaaagt atcccgaaag cgactaacca
    29101 ggttatctac cgcatcctga ttttctatat tggttcgtta gccgttctgc tctcactgat
    29161 gccgtggacc cgcgttaccg ccgataccag tccgtttgtg ctgatcttcc acgagttagg
    29221 cgataccttt gtggcgaatg cgctgaacat cgtggtactg actgcggcgc tctccgtgta
    29281 caacagctgc gtatattgca acagccgtat gctgtttggt ctggcacaac agggtaatgc
    29341 gccaaaagcg ctggcgtctg tcgataaacg cggcgtaccg gttaacacca ttctggtgtc
    29401 tgcgctggtt acagcattgt gcgtattgat taactatctt gctccggaat ccgcatttgg
    29461 cctgttaatg gcactggtgg tatccgcact ggtgatcaac tgggcgatga tcagtctggc
    29521 gcatatgaag ttccgtcgcg ccaagcagga acaaggcgtg gtaactcgct tccctgctct
    29581 gctttatccg ctgggtaact ggatctgcct gctgtttatg gcggcggtac tggtgattat
    29641 gctgatgacc ccaggaatgg cgatttcggt atacctgatc ccggtatggc tgatcgtgtt
    29701 aggtatcggc tatctgttta aagagaaaac cgccaaagcc gtaaaagcgc attaatctct
    29761 ctacgccctc acccatgcag ggtgagggca ataatcttta caccaacgct ccgtaaatcg
    29821 tcagtagcgc aatcaccacc acgaccacga acgaggtttt cttcgccatt gaaaccgctg
    29881 ccttcggcgt ctccacctta tcgacatgcg gttcacgcgc cagagagaac tgcgccagac
    29941 gcgttaacac ctgatactgc gaagtatgga agtcacccag cgaagcaaac caggccggta
    30001 acgctttctc cccatgaccg atcaaggcat ataccacacc cgcaagacga accggcaccc
    30061 aatccagtac atgaagcacg gcatcaatgc cggactgtaa acgatgatgc ggcgtctggt
    30121 atcgcgccag ccagtattgc catgcacgca aaaacgcata ccccatcagc gtaacgggtc
    30181 cccaggttcc ccccacaatc aaccagaaca gcggtgcaag ataaaaacga aagttaatcc
    30241 acagcaatgc attttgcagc tcacgcaaat actcacgttc gtcgcagcct gccgggacgc
    30301 cgtgaatcat ggtgagttcg ccagccatcg tggcacgggc atggctatca ttacgtgaag
    30361 cagctgtcag ataagcatga taatgaagac gaactttacc tgcgccaata cacagcaaac
    30421 caatcagcag ccacaccagc agcgtgggaa cgttgaacaa tactccctgc aatgcgcgta
    30481 acagtaaaaa agtcacgccc atcgcaataa tggtcatgcc taacgtgcgc ccgagagaaa
    30541 aatgtttcac ccgccgaaag aacgcttcaa gacgatgatc aagctgccag tgctcgccca
    30601 acttaaacag gcgctcgaaa attaacacca gtaaggttgt aaatagcgtc atgttgtctc
    30661 cttgctgacc agcgcacgaa accgtgccca atcaaatgca ggaccgggat cggttttccg
    30721 atccggcgca atatcacaat ggcccgtcat gttattagcg atatcgggat agcgatctat
    30781 cagtgcccgc gtaaccgccg caagctgttg atactgcgca tcggtatacg ccagagtatc
    30841 ggtgccttca agctcaatcc caatagaaaa atcattgcag cgttcacgcc cctgatactg
    30901 agagactccc gcatgccagg cacgtttatc gaaaggaaca tactggacta tttcaccatc
    30961 acggcgaatc aaacagtgag cggagacgcg caaatgggcg atctcagcaa agaaaggatg
    31021 tgcctgcgga tcaatagttc cagtgaataa tgcgtcgatc cacggaccgc caaactcgcc
    31081 tggcggcagg ctaatattgt gcaccaccag cagggtgggt gtttcgtcat ccgggcggca
    31141 atcgtaatgt ggtgagggaa cgcggcgcgc gccaaccagc cacccctgtt ctaacaacat
    31201 gcaggatctc cttatatgtg gtgctaatac ccggttcaga gtagcatgtt tctaccttat
    31261 gattcgttag ctatctggag ttttaacatg ccgcctcgcc gctataaccc tgacacccga
    31321 cgtgacgagc tgctggaacg cattaatctc gatatccccg gcgcggtggc ccaggcgctg
    31381 cgggaagatt taggcggaac agtcgatgcc aacaatgaca ttacggcaaa acttttaccg
    31441 gaaaactctc gctctcatgc cacggtgagc acccgcgaga atggcgtatt ttgcggcaaa
    31501 cgctgggttg aagaggtgtt tattcaactg gcaggcgacg atgtcaccat aatctggcat
    31561 gtggatgacg gcgatgtcat caacgccaat caacccttgt tcgaacttga aggcccatcc
    31621 cgcgtgctgt taacgggcga acgcactgcg cttaattttg tgcaaaccct ttcaggagtt
    31681 gccagtaagg tacgccacta tgtcgaatta ctggaaggca ccaacacgca gttgttggat
    31741 acgcgcaaaa ccttacctgg cctgcgttca gctctgaaat acgcggtact ttgcggcggc
    31801 ggagcgaatc accgtctggg gctttctgat gccttcctga tcaaagaaaa tcatattatt
    31861 gcctccggct cagtgcgcca ggccgtcgaa aaagcgtcct ggctgcaccc ggatgcgcca
    31921 gtagaagtcg aagtagagaa tctggaagaa cttgatgaag ccctgaaagc aggagccgat
    31981 atcatcatgc tggataactt cgaaacagaa cagatgcgcg aagccgtcaa acgcaccaac
    32041 ggcaaggcgc tactggaagt gtctggcaac gtcactgaca aaacactgcg cgaatttgcc
    32101 gaaacgggcg tggactttat ctccgtcggt gcgctaacta aacacgtaca agcactcgac
    32161 ctttcaatgc gttttcgcta attgctgaca gatttatccg ctccatcgcg ggcggataat
    32221 ctccccttcc ccaactttct tcgtaacgcc tcgcaaattt cttgtctttt gctgcaacga
    32281 aataaaaaac ctcggaagct gctttcccgt ttcgtttttt gcccctcgct tgtgctgatg
    32341 gcgtttgcca aagtagcgcc aaccaaatca aggagcgaaa cagatggaca agcaacgcgg
    32401 ttttacactt atcgaactga tggtggttat tggcatcatt gccattttaa gcgccattgg
    32461 tattcccgct tatcaaaact acctgcgcaa agctgcactc accgacatgc tacaaacctt
    32521 tgtgccttac cgtacagccg tagagttgtg cgcgctggaa catggtggat tagatacctg
    32581 cgacggtggc agcaatggca ttccctcgcc taccaccacc cgctatgttt cagccatgag
    32641 tgtggcaaaa ggcgtggtgt cgctgaccgg gcaagaaagt ctcaatggac taagcgtcgt
    32701 catgacaccg ggttgggata acgcaaacgg cgtcaccggc tgggcgcgca actgcaatat
    32761 tcaaagtgac agcgcattgc agcaagcctg cgaagatgtc ttccgctttg atgacgccaa
    32821 ctaaggagcg gcaatgaata ttccacagct caccgccctg tgtctgcgtt atcagggagt
    32881 cttgctggat gccagcgaag aggtggttca tgttgcggta gtcgatgcac cttcgcatga
    32941 gttgctggac gcattgcatt tcgctaccac caaacgtatt gagatcacct gctggacgcg
    33001 ccaacaaatg gaaggccacg ccagtcgcac acaacagaca ttgcccgtag ctgttcagga
    33061 gaagcatcag cccaaagcag agttgctgac tcgaacgtta caatctgcgc tggaacaacg
    33121 tgcgtctgat attcatatcg aaccagcgga caatgcctac cgcatccgct tgcgtatcga
    33181 cggcgtattg catcctttac cggatgtttc accggatgcc ggagtcgcat taaccgccag
    33241 attaaaagtg ctgggaaacc tggatattgc ggaacatcgc ctgccgcagg acgggcaatt
    33301 cactgtcgaa ctggcaggaa acgccgtctc atttcgtatt gcgaccttac catgtcgggg
    33361 tggtgaaaag gtggtattaa ggttgttaca gcaggtgaac caggcactgg atgttaacac
    33421 gctgggaatg cagccgttac aactggcgga ctttgctcat gccttgcaac aaccacaggg
    33481 actggtgctg gtaactggcc ctacaggcag cggcaaaacg gtcacgcttt atagtgccct
    33541 gcaaaagctg aataccgctg acattaatat ttgtagcgtc gaagatccgg ttgagatccc
    33601 catagccgga ctaaaccaga cgcaaatcca tccgcgtgcc gggctcacct ttcagggcgt
    33661 tttgcgtgcg ttattgcgcc aggatcctga cgtcatcatg atcggagaga tccgctatgg
    33721 ctttaacggc agaaattgcc attaaagccg cgcaaaccgg tcacctggtg ttgtctaccc
    33781 tacacactaa ttccacctgc gaaacgctgg tacgtttaca gcaaatggga gtcgcccgct
    33841 ggatgctctc atcagcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc
    33901 cacattgtcg ccagcagcaa ggggagccca tccatattcc agtcaatgta tggccgtcgc
    33961 cgctgcccca ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta
    34021 cggccttatt tgaagttctg cccataacgc cggtcattcg tcagcttatt tccgctaata
    34081 ccgacgttga atcgctggaa acgcacgcac gacaggcggg tatgcgtacg ctttttgaaa
    34141 acggctgcct ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg
    34201 ggatgccgca tggcgagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat
    34261 gcgcaagatg ggatgctatg agcagagagc cgtgctttgc tgctcatggc actacagcaa
    34321 cagatggtta ccccacttag cctgaagcga atcgccatca attctgcgca gtggcgagga
    34381 gataaaagcg cggaagtcat tcatcaactg gcgacgctac tcaaagccgg gttaacgctt
    34441 tctgaagggc tggcactgct ggcggaacag catcccagta agcaatggca agcgttgctg
    34501 caatcgctgg cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg
    34561 tcagaggtat ttccgccact ctatcaggcg atgatccgca cgggtgaact gaccggtaag
    34621 ctggatgaat gctgctttga actggcgcgt cagcaaaaag cccagcgtca gttgaccgac
    34681 aaagtgaaat cagcgttacg ttatcccatc atcattttag cgatggcaat catggtggtt
    34741 gtggcaatgc tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc
    34801 ccactaccgg cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggagc
    34861 tggctgctgg tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccga
    34921 ccgacctggc ttatagtgcg gcagaaattg ctgttacgca tcccgattat gggttcactg
    34981 atgcggggac aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc
    35041 attacttttt tgcagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg
    35101 caacttctga cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta
    35161 aaaaataccg gggagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc
    35221 ggctcgctgg acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg
    35281 ctggcggata acctcgcagc cttactggaa ccgacgttgc tgatcataac gggaggaatt
    35341 atcggtacgc tggtggtggc gatgtatctg ccaattttcc atttaggcga tgcgatgagt
    35401 gggatgggat aacgctggcg tgttgctcca caccagcgtt ggaagattac aggttgttga
    35461 agatgcggtt ttcttgttcc tgcacacgga taaatgtggt gcgcttggtc agttctttca
    35521 ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg
    35581 tattttcaac cgggcctcgc agcggcagct taacggtttt accttctgct gcgcgatatt
    35641 ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca
    35701 taaatttctc gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca
    35761 tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc ttttgcgaca tcgcccggcg
    35821 tggtgcagcc accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt
    35881 cgattaccgc agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag
    35941 aacctgggcc aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca
    36001 tttcaccagt cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt
    36061 tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt
    36121 ttaatgccgg gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtac
    36181 cggtagaaac catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc
    36241 actcttcgac agaatagtgt ttatgcacag cagtcaaaat atcaaaagaa gccagcgcag
    36301 aggccataga aaatgtacct acggtgtcca tatttgcggc gataatcggc acgccggacc
    36361 agctctgacc tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt
    36421 taagagtgga gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt
    36481 caatacgcat gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt
    36541 gacgctatca tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc
    36601 tacaatccca taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcgctttcg
    36661 tgcactcatt tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgagg
    36721 tatatagttg ccttaacggg aggcattggc agtggcaaga gtaccgttgc caatgcgttt
    36781 gctgatctcg gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca
    36841 ggtgcacctg cgctacatgc cattgctgat cactttggcg ctaacatgat tgctgctgat
    36901 ggaacattgc agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac
    36961 tggcttaacg ccctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa
    37021 gctacttccc cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa
    37081 aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg
    37141 cagcgcgatg atgtaactcg cgagcatgtc gaacaaatcc ttgctgctca ggcaacgcgc
    37201 gaagcccgcc ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc
    37261 gcatcggatg ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca
    37321 caggaaaaac cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc
    37381 gtacatggct gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg
    37441 accacgctgg cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc
    37501 gcggcgaagt ccgcactgag ctgttgaaag aacttgaccg gcagcaacgt aaactccaga
    37561 cctggattgg cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa
    37621 aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc
    37681 gtttgattgc tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt
    37741 tacctacatt gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa
    37801 cctggattgc cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc
    37861 gccagtcggc ccccttccgt aaacaaacca gtctgaatgg tttttatcag gataacggtg
    37921 gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt
    37981 ccggacataa gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg
    38041 taccggaacg tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta
    38101 ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc
    38161 ggccattttg ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa
    38221 aacgaatccc cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc
    38281 agtgacattt gtctgatgcc gcacgtaggc ctgataagac gcggacagcg tcgcatcagg
    38341 catcttgtgc aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc
    38401 ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg cattaagacc gactagcgac
    38461 atccactcac cgggttgccc ttctttaccc cacggctccc cttcccagct ttcgaccagc
    38521 cagaaccaca gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa
    38581 aaatgttgtg gggtaatccc gacttcttcc tgaagttcac gcactaccgc ctgttccggc
    38641 gtttcgccca tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct
    38701 gctgcgcgac gcgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt
    38761 tgcagctttt tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt
    38821 caactgttag cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc
    38881 acgggcaagg atcgttacgt cctactttgc gctcaccggt ttgcgccgcc agtgcagctg
    38941 cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat ttgcgctaaa cgctcggctt
    39001 ccatacgacg ctgttgttcc agctcctcaa cctcttcagg catacgtacc tggactttgc
    39061 tcagcgtgct gataacttca tattttaacg actccagcat cgctgcaaac atggagaacg
    39121 attcacgttt gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct
    39181 gacgcagata gtccatcgcc gctaggtgct ctttccacag agagtcgaga gtttgcagca
    39241 tgacgccttt ttcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat
    39301 acacttcgat ggactgcgcc agaattcgct cacgcagcgt ctcttcatgc agttctggtt
    39361 ctttatccag ccactcggca attggcaaat cgaggtcgaa atcgttcttc agacgttcct
    39421 gcagccccgg aatatcccac atttcttcca gtgactgtgg cggaatgtag gcatcaatgg
    39481 tcgctttgaa cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca
    39541 gcagttcgtt acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt
    39601 ccagcagttg cttacgaatg tcgaagttac ggctttcaac tttacgctgg gcgttggcaa
    39661 tcgctttagt cacccacggg tgttcaatgg cttcgcctgg cttcataccc agtttacgca
    39721 tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt
    39781 agaaacggga agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga
    39841 tacgacggga ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg
    39901 catcgtgacg tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt
    39961 ccagcgcggc aacttctgcc tgccagctac caccgagcac aatatctgta ccacgacccg
    40021 ccatattggt cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt
    40081 cgttggcgtg gaatttggcg ttcaggacgt tgtgcttaat accggctttg gtcagttcgt
    40141 ttgacaccag ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg
    40201 cagtacgttc tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga
    40261 ccaggtccgg cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca
    40321 gcttgtagat ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca
    40381 gtttttcata cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt
    40441 tctggatctg cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgac
    40501 ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga
    40561 tgtagtcgac gtcgcgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca
    40621 tcagcatgat gttggccgga gagtacagag actccccttc atccatgatg ccctctttca
    40681 ccagcagttc ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt
    40741 cgtccaccga gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga
    40801 tcaggtgcgg aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc
    40861 cggaaatgat cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca
    40921 gcgcatagtg cagtttacgc tgtacacgtt cttcagggct gaacgccata ttgtcgcgca
    40981 ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc
    41041 gctttgccgg tgctggcatg cccggcaggt tgataccgac agtcaggcca aggaattcaa
    41101 acagcggacg gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt
    41161 gcacgccttt accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac
    41221 cttcaccggt acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact
    41281 gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc ctcacgtacc acggcgaaag
    41341 cttccgggat cagattttcc agcacttcgc ctttttccag acgtgcacga aactctgcgg
    41401 ttttcccttt cagttcttcg tcggagagtt tttccatctc cggttccatg gcattgatga
    41461 tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag
    41521 ttaacaattt gattagcata ataaaatctc aaacgccccg cgttgcggag ttaataaaat
    41581 gaagtaaagg tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc
    41641 tggcttatcc agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg
    41701 cgataaccct tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt
    41761 aatgcaagat gttgcgcctg aagaggcaaa gactcttcag caacgggcag tgtttgcggt
    41821 gccattgcga aagaaagatg acggattacc gtgcgaatgg catgttgatg ccagtaatca
    41881 acggaatagt tcgaattcgg gcggcgtgtg ttcgcttcca gcaaggccaa ttgaccaaag
    41941 ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg
    42001 gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcaa ccatccctaa taagagatgc
    42061 ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg
    42121 ccatcccgat cgtattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgcagtc
    42181 cacagcacga atgaagataa gttgtgcgga aaagtgctta agaaaacagc gttcgcaccg
    42241 ttttttatag ctcttataaa attcaaaaaa aaatgactgg ttcacctggc cggagagagt
    42301 gccagatttg ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg
    42361 aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg
    42421 cttcctgttt cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat
    42481 aagcggtaaa tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga
    42541 gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat
    42601 aatcgtcaac aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca
    42661 gatattcgat atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg
    42721 catcagcgga gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa
    42781 aatcgatggt gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc
    42841 catcttcgac acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt
    42901 cgataccggc gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga
    42961 tttccggcgc gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat
    43021 tgaggtgctc tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca
    43081 tggtatcacg cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc
    43141 gacgatagat gaccccggtg ttggccggcg cagggcgtaa cgtcagagtg actttcttgc
    43201 cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca
    43261 tcgtattatc tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag
    43321 tttagcacaa agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgc
    43381 aggaatgctg ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc
    43441 acgactttag caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag
    43501 cgatccatca ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg
    43561 tccatgccga tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca
    43621 agagaagtac cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct
    43681 accgtttcga actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg
    43741 cgcgcgccag acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct
    43801 tcttccgcac ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatctca
    43861 gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt
    43921 tcagcgatac cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc
    43981 agggagatac cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag
    44041 tccacatgct tggacagttc agtgatcccc tgctccgcga atgccatacg cttcttgcct
    44101 tcaaagttga aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact
    44161 tcagcgacga ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag
    44221 accatgtctg caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg
    44281 cgaccaactt ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga
    44341 atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat
    44401 tcaacacctt caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg
    44461 acgccgatga ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct
    44521 ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac
    44581 tcttttcgca gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt
    44641 ttttctactt cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc
    44701 gccgtcgaat aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg
    44761 ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc
    44821 gctgcgccac cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga
    44881 agcttttcct gcaactgcaa tatctcttcg ttgaccaggt tgagcagctc ggtatagcgc
    44941 ggctcgatca cctctgccag ggtctgacgt tgcagactcc gtggcggacg accgcctacg
    45001 ctcggcactt ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga
    45061 actttaatcg cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc
    45121 acgacattgc cagcataagg aattacctta gtgtggcgca atgccccacc ggtataaacg
    45181 gcgatatcca ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct
    45241 tccgtcaata ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc
    45301 ccacaacgtt caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg
    45361 tgcacttttg cctgcatccg cacgcccgaa agtcctaccg gattcttgat cccttcctga
    45421 tagtcaatcg catactcttg cgggatcaca tgcagcacac gatgttcatc gcgcacacgc
    45481 accgatttcg cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa
    45541 ataggcacca taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat
    45601 accgaagaga tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac
    45661 ttgaccacgg attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg
    45721 cccacgccaa tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc
    45781 ttcgcggtac caatctccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt
    45841 gttctgcctg tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc
    45901 ctactgccgc tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt
    45961 gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg agccaaacgt ttcatcgtat
    46021 cgccccggcc aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg
    46081 cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc
    46141 gatagccctg caacacttca ttggcgctgc cttccgggcc atacagcatt ggaagcacct
    46201 gcttgctggt gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatatgtt
    46261 gatcattcca ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc
    46321 actgctttct gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga
    46381 tgatgttgac atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc
    46441 ggatatcgtc attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca
    46501 ggcgttgcgc atcttccatc cagcccaaca cgacccagcc gctcaccaac actgtcgtta
    46561 aaacggtcag caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa
    46621 cctcttcttc gctatttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc
    46681 agaattcgta ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc
    46741 aggctgtggc tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatcg
    46801 ctgtccagca taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc
    46861 agcactaatg cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg gcagaaatac
    46921 tgtgtctcat cagagagata cttcgcctca taatcataga aggttccgga cggttgaata
    46981 cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc
    47041 catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga
    47101 gcattttctg ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg
    47161 ataaccggca aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca
    47221 aactctgcgc gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacagc
    47281 agtttgctgc gtagtttatc cattgaaagc gcagatgcca tcactccgct gccggtataa
    47341 ggcaagccca tcagctcaag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt
    47401 agcgcgataa acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc
    47461 gggtcgacag gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca
    47521 gaattcagag aaacttcccg ctcagcggaa gtcccgccca acaggaccgc aattttatca
    47581 gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc
    47641 aatttttccg atattaccag ccccctgaac gagaatcagg tcgttaccga ttaataccgg
    47701 tgccagcatc tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg
    47761 tccacgaatt gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc
    47821 cggatacact tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc
    47881 atacaggtcg cgcgtacggg taaaacggtg cggctgaaac agcattacta ggtttttatc
    47941 cggccagcct gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta
    48001 gtcatcgacc agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc
    48061 gaggaaatca aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc
    48121 gtcgtcaatt ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg
    48181 acggcctggc gcattcaggg tgacgcgcat cggctctttg tcctggcgca gcagcgtaaa
    48241 gtgcccctgc gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa
    48301 gccgtaagtc gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc
    48361 aacacacatc accgcacgac cgtaaaacgg caggttgtgc agaaaattaa taaaagtctg
    48421 ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac
    48481 aatcgccacc atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat
    48541 caggtaccga ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt
    48601 aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtggttgt
    48661 cgttttgccg tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag
    48721 catttcggca cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt
    48781 atcggcagaa atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg
    48841 atggttgaaa taaatcgtcg ctcccagatt cattaactgc tgcgtgaccg gatttggcgc
    48901 taaatcggaa ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat
    48961 accggcacca ccgatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat
    49021 ggaacgcagt tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc
    49081 atacgatgca aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc
    49141 actcgctcgg tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag
    49201 gtttctcgcg accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc
    49261 tcgataattt tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg
    49321 tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcacc
    49381 gttaaagcac cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca
    49441 tcaataaatt ccgtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg
    49501 gattgttgcg aacctttacc gctctgatgc cagatagtga ctgaatcacc cagtttcgca
    49561 gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccagc
    49621 acacgaaccg gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca
    49681 tcggtacgca ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc
    49741 atcactttgg tggcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt
    49801 tcatgaagta caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca
    49861 tagccgccca taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga
    49921 cgccaggcgt tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc
    49981 agaccagaga tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc
    50041 atacggtcgg cagtccccag ccagcgaact tgccaaccct gagccattag atggtgcgca
    50101 accgccagtc ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt
    50161 ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat
    50221 aatcaatacg caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt
    50281 aactgatcag cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt
    50341 taaccagcgc ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac
    50401 ggtggtcaat ttctaacgct ttgcggccaa tcgacatcgc gcgaaaagcg acgaagaata
    50461 ccattaaaag tgccagcacc acaccgacat accccagttc ttcgccgata atggcgaaaa
    50521 taaagtcagt gtgcgcttcc ggcagatact ccagtttttg taccgagtta cctaaacctt
    50581 gtccccaaag ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc
    50641 caaagggatc ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg
    50701 cgagtatcag caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca
    50761 atttcgctcc cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac
    50821 caaggtctgg ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca
    50881 ggaagccgcg caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat
    50941 aacaaaacag cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga
    51001 tccaacgcga tgccccttta accgagctac ccactaccag cacgatcatc agcaggatga
    51061 tagatccgag cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg
    51121 taatgatcgc cagaatgaat gccaggatca gatagacgcc atcacgcttc gcgaagaaga
    51181 acggatcgtt ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc
    51241 caatcgccgc aaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt
    51301 cggtatcttt ttcccgcgag cccatcaccc agccttttag cgccgtggag atccagacca
    51361 ggatactgaa tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc
    51421 taactccttc gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc
    51481 aaggctggca caggccgggg agagcagaac catatcgccc agctgaacac gcggagccag
    51541 caagcgcatc gcttgttcca tggtttcggt ttgttctgcc acttccgggc gtagcgccgc
    51601 cagctgcgca ccgtcacgac caaaacagta caaacgcacg ttgtcgccat tcagatagcg
    51661 cgccagtgga ctgaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc
    51721 gtctacctgc aagccattca acgccgcttc cgtactgccg acgttagtgg ctttcgaatc
    51781 gttaatccag cgtacgccat tgtgctccag caccacttca aagcgatgcg gcagaccggt
    51841 gaaggtggtt aacgctttca ggctgctggc gcgcggcaaa ccagctgcgt ctgccagtgc
    51901 cagtgctgcc agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt
    51961 cagcactttc tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata
    52021 gtcacccatg ttgacgccaa agctgacgca gcgttcatca gcaccgcgaa tcggcattgt
    52081 taaggcatca tcagcattga cgacacaaac tttcgcattt tcgtaaatac gcaattttgc
    52141 cgcacggtat tgctgcaaac caaacggata gcggtccata tgatcttcag tcacgttcag
    52201 aatggtcgcc gccaccgcct gtaagctgga agtggtttcc agctggaagc tcgacagttc
    52261 cagcacgtac agttcgcact cagcatcaag cagcatcaac gctggcaggc caatattgcc
    52321 acccacacca acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt
    52381 gcttttgccg ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacagaacag
    52441 ctcgatatcg ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc
    52501 ctgtgcaata ccgggactgg cgacaatcag atctgccgcc atcagccatt catcattcag
    52561 actgcccgtg tggcgttcta cggcttcggg taatttatcc aggccaggcg gtgtcatacg
    52621 cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc
    52681 ggtgaggccc aggccgataa tgacgacatt tttaccctga taatcagcca tgattaacgt
    52741 accttcagcg ttgccagacc aatcagaacc agcatcagcg aaataatcca gaaacgcaca
    52801 atgacgcgcg gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg
    52861 aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct
    52921 accacgaaca cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata
    52981 atgcctaacg caccacctaa cgccagcgaa cctacatcgc ccataaagac ctgcgccgga
    53041 taggtgttaa accacaggaa gcccagtcct gccccgacta tcgccgtgca gacaatgacc
    53101 agttcccctg cgtgtcgcag atacggtata tgcaagtagc tggcaaagtt catattgccg
    53161 gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccagg
    53221 ccgtcgagac catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc
    53281 agcagaatgt agaacagccc cagctgcggc atcacatctt taaagaatgg gaccaccagc
    53341 tgcgttgcgg gcgtgtcttt gccggcaagg tacagggcga aggcgacacc cagcgcaatg
    53401 accgacatcc agaaatactt ccaacgagcg atcaaccctt tggtgtcttt acgcaccact
    53461 ttgcgataat catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac
    53521 cagacgtacg gattggacgg gtaagcccac agcagtacgg agatcacaat cgccgtcagg
    53581 atcataatcc cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg
    53641 ttacgcacca cctgaccaaa ggaaagtttt tgcaaatgag ctatcatacg cgggcccatc
    53701 cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa
    53761 aagacgttaa agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc
    53821 cattctcctg taaagcgcgt actacctctt ccatggcggc actacgtgaa cccttaacta
    53881 aaatcgtaat tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt
    53941 tatcagcaaa atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca
    54001 cgcttaacac gcggtcaata ccagcagctt ttgccgcctc gcccacctgt acatggcagg
    54061 cttcgctttc agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca
    54121 tttcagccag tacctgaaca gcggcggtca ttgaaccgac attggcgttg taggagtcgt
    54181 cgagcagcaa ctggttttct gccagttgga tggggaacag acggcctgga acagctttca
    54241 gatttgccag ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag
    54301 cggctgccag cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc
    54361 taccggttgg ggtttgtagg gtaaattccg taccgtgcga ggtcacatgg atattggtgg
    54421 cggtgaaatc gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta
    54481 cgctctgcca gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg
    54541 gcaggccgct aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt
    54601 ccagatgcgc cgctgccagg ttgttgacca gcgcagcttc cgggcgagtc agactcacag
    54661 tccaggctat ttcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg
    54721 gcgtcaagcg caacaacgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat
    54781 aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc
    54841 cggaggaacc tgtcagagca accacgcgcg ccggaacttg ctggcgaacc catgcagcca
    54901 gttcaccaaa cgccagacgc gtatccttga cgattaactg cggcaggtcg atatccagcg
    54961 gacggctgac cagtagtgcg cccgcgccgc cagctttcgc ctggtcggca aaatcatgag
    55021 catcgaaacg ttcgcctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat
    55081 cagtggttac agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatgtcgg
    55141 taagttggct aagggttacg ctaatcatgc aatcaccccc agcagacgcg ccaccgtgac
    55201 gcgatcggag tagtccagac gctgattgcc aacaatctgg taatcttcat ggcctttgcc
    55261 cgcgaccagt accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc
    55321 acggccttcc atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat
    55381 gatggcacgc ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa
    55441 ctcttcggca attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc
    55501 aaagacgcac cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag
    55561 tgcatccggc gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac
    55621 ttccatacgt ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg
    55681 atagccgagt gccagcagtg tcgccagcgc gagcagcagg ttgctgacgt taaaagcgcc
    55741 catcagatgg ctttcaattt cgccatcgcc ccaacttgag ctaaagcgaa tcgtcgcacc
    55801 gctgtcgtga tagttcacgt cgatcgcttt taaccagcgt ccgtgacagt tcggattaat
    55861 atgatcttcc attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac
    55921 ttcatcgtcg gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt
    55981 cgcagcttcg tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt
    56041 aaagaccgac gccgcaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga
    56101 aacttccatt gcgcaaaacg tcgcgccctg ctccaccagc cccgccagct catgctgaac
    56161 atcgactgcg gaaccggtag tattttctgt cgggatcact ttcccaagca gaccgttacc
    56221 aacggtgccc attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagcagctg
    56281 ggtagtcgtg gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg
    56341 ttcatggtaa aagcggcccg ccagtgcaga taaacgctca ttgagctggc tgagatagat
    56401 gaccggtacg ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgctttcgc
    56461 ctctgcaata atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc
    56521 cgcctgatga cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag
    56581 tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg
    56641 caaattacga tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc
    56701 ccgttgtcag cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac
    56761 caaagaccgg cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa
    56821 caacaaccag cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat
    56881 atttattgat gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga
    56941 tacgatagcc tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca
    57001 tcatatgcac cacagtgcgg acaatggatc ccgggaagac acgttcaccg ggaaccgggg
    57061 ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga
    57121 ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct
    57181 ctatgtcaga ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca
    57241 aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca
    57301 tcgctaacgc cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg
    57361 ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg
    57421 tattgagtac cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatt accaccatcg
    57481 gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt
    57541 tcggcgtgcc gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt
    57601 caccggtgtt gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg
    57661 cgttgttcag ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt
    57721 tgtgcgctgc ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt
    57781 tacgcacaat gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc
    57841 cctcaatccc ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg
    57901 acggatagta acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga
    57961 tgtagtccgc catgtcaggg ttcacctgac gcgccagata aataaaacgc cctttcgggt
    58021 tggcgttaat gcgggctgaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct
    58081 tccagcggtc accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa
    58141 tcgcttttac cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc
    58201 gggaggtgga aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat
    58261 ccggggagat aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa
    58321 tacagccgca taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt
    58381 ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta
    58441 cgatattttc ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt
    58501 ccacccggct atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt
    58561 ctaaagcatc tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt
    58621 gcgccgtggt taccacagtc accgccgtca aaataatgca aatgaacagg cagagtggca
    58681 gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc
    58741 ttcccatcga tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcattcgt
    58801 cctctctgca atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc
    58861 gcccggcatt aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc
    58921 ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg
    58981 tttcacaata cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc
    59041 cagcacgttg agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca
    59101 aatgcgcacc gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt
    59161 tgcaacagcc acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg
    59221 ctcgacaatg gcgcgggcaa tgcgtttggc aaaacgctct tcaccatagg ttttcaatac
    59281 ccaagcgata tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt
    59341 tgggtccata cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gctcagcatc
    59401 atcaagttgc ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccgataag
    59461 atcgcgctcg gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg
    59521 cggatcatca atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa
    59581 acgcccctct tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt
    59641 cccatcaatg tagatgccat caggacggat attgaggcca ttaacggctt catccagcag
    59701 caccgtagta tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg
    59761 ataagtctcc ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg
    59821 ttgtttcatc ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca
    59881 gcccggcatg ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac
    59941 attcgctggc atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctcg
    60001 acagacgcga taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc
    60061 acgggtgatg aatgtcaatg gtgcaaacca tttgaccgac agcgttctca agcagctgtt
    60121 cccggtaacg ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc
    60181 cccggaacat gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag
    60241 gagtttacgg agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa
    60301 acccgatgtt tacggctaat atcagcgttg tttattttac gtccaacgtg cgcagcaaat
    60361 taacgttatg aatagctgta agaaaaaatc cctcatcgtc atcaccgttt taaaatgact
    60421 cactattttc cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca
    60481 tatcttc
//
LOCUS       DAJTYZ010000006        59841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000006 DAJTYZ010000000
VERSION     DAJTYZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.006"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..262)
                     /locus_tag="OO596_000358"
     CDS             complement(<1..262)
                     /locus_tag="OO596_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6716320.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="OO596_000359"
     CDS             369..665
                     /locus_tag="OO596_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6716321.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OO596_000360"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OO596_000360"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6716322.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OO596_000361"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OO596_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6716323.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OO596_000362"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OO596_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6716324.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="OO596_000363"
     CDS             complement(3472..3645)
                     /locus_tag="OO596_000363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716325.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /locus_tag="OO596_000364"
     CDS             3673..3756
                     /locus_tag="OO596_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6716326.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OO596_000365"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OO596_000365"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6716327.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OO596_000366"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OO596_000366"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6716328.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="OO596_000367"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="OO596_000367"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6716329.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="OO596_000368"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="OO596_000368"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6716330.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OO596_000369"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OO596_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR6716331.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OO596_000370"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OO596_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6716332.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="OO596_000371"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="OO596_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6716333.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OO596_000372"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OO596_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6716334.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="OO596_000373"
     CDS             14052..15254
                     /locus_tag="OO596_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6716335.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16553)
                     /locus_tag="OO596_000374"
                     /pseudo
     CDS             complement(15570..16553)
                     /locus_tag="OO596_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            16953..18560
                     /locus_tag="OO596_000375"
     CDS             16953..18560
                     /locus_tag="OO596_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6716336.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            18822..20444
                     /locus_tag="OO596_000376"
     CDS             18822..20444
                     /locus_tag="OO596_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6716337.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            20810..21877
                     /locus_tag="OO596_000377"
     CDS             20810..21877
                     /locus_tag="OO596_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6716338.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            21874..24609
                     /gene="rbbA"
                     /locus_tag="OO596_000378"
     CDS             21874..24609
                     /gene="rbbA"
                     /locus_tag="OO596_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6716339.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            24609..25733
                     /locus_tag="OO596_000379"
     CDS             24609..25733
                     /locus_tag="OO596_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6716340.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            25806..26079
                     /locus_tag="OO596_000380"
                     /pseudo
     CDS             25806..26079
                     /locus_tag="OO596_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            26076..26435
                     /locus_tag="OO596_000381"
     CDS             26076..26435
                     /locus_tag="OO596_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6716341.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OO596_000382"
     CDS             complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OO596_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6716342.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OO596_000383"
     CDS             complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OO596_000383"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6716343.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OO596_000384"
     CDS             complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OO596_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6716344.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OO596_000385"
     CDS             complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OO596_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6716345.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OO596_000386"
     CDS             complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OO596_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6716346.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OO596_000387"
     CDS             complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OO596_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6716347.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OO596_000388"
     CDS             complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OO596_000388"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6716348.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(32630..33679)
                     /locus_tag="OO596_000389"
     CDS             complement(32630..33679)
                     /locus_tag="OO596_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6716349.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            33811..35028
                     /locus_tag="OO596_000390"
     CDS             33811..35028
                     /locus_tag="OO596_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6716350.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OO596_000391"
     CDS             complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OO596_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6716351.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(35662..36327)
                     /locus_tag="OO596_000392"
     CDS             complement(35662..36327)
                     /locus_tag="OO596_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6716352.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            36548..36793
                     /gene="tusA"
                     /locus_tag="OO596_000393"
     CDS             36548..36793
                     /gene="tusA"
                     /locus_tag="OO596_000393"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6716353.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OO596_000394"
     CDS             complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OO596_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6716354.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(39167..39793)
                     /locus_tag="OO596_000395"
     CDS             complement(39167..39793)
                     /locus_tag="OO596_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6716355.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            39934..40293
                     /locus_tag="OO596_000396"
     CDS             39934..40293
                     /locus_tag="OO596_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6716356.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(40296..40565)
                     /locus_tag="OO596_000397"
     CDS             complement(40296..40565)
                     /locus_tag="OO596_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6716357.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OO596_000398"
     CDS             complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OO596_000398"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6716358.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            41301..42797
                     /gene="ftsY"
                     /locus_tag="OO596_000399"
     CDS             41301..42797
                     /gene="ftsY"
                     /locus_tag="OO596_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6716359.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            42800..43468
                     /gene="ftsE"
                     /locus_tag="OO596_000400"
     CDS             42800..43468
                     /gene="ftsE"
                     /locus_tag="OO596_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6716360.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            43461..44519
                     /gene="ftsX"
                     /locus_tag="OO596_000401"
     CDS             43461..44519
                     /gene="ftsX"
                     /locus_tag="OO596_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6716361.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            44764..45618
                     /gene="rpoH"
                     /locus_tag="OO596_000402"
     CDS             44764..45618
                     /gene="rpoH"
                     /locus_tag="OO596_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6716362.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            45889..46992
                     /gene="livJ"
                     /locus_tag="OO596_000403"
     CDS             45889..46992
                     /gene="livJ"
                     /locus_tag="OO596_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6716363.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OO596_000404"
     CDS             complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OO596_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6716364.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            47998..49107
                     /gene="livK"
                     /locus_tag="OO596_000405"
     CDS             47998..49107
                     /gene="livK"
                     /locus_tag="OO596_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6716365.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            49155..50081
                     /gene="livH"
                     /locus_tag="OO596_000406"
     CDS             49155..50081
                     /gene="livH"
                     /locus_tag="OO596_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6716366.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            50078..51355
                     /gene="livM"
                     /locus_tag="OO596_000407"
     CDS             50078..51355
                     /gene="livM"
                     /locus_tag="OO596_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6716367.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            51352..52119
                     /gene="livG"
                     /locus_tag="OO596_000408"
     CDS             51352..52119
                     /gene="livG"
                     /locus_tag="OO596_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6716368.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            52121..52834
                     /gene="livF"
                     /locus_tag="OO596_000409"
     CDS             52121..52834
                     /gene="livF"
                     /locus_tag="OO596_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6716369.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            53118..54182
                     /gene="ugpB"
                     /locus_tag="OO596_000410"
                     /pseudo
     CDS             53118..54182
                     /gene="ugpB"
                     /locus_tag="OO596_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            54280..55167
                     /gene="ugpA"
                     /locus_tag="OO596_000411"
     CDS             54280..55167
                     /gene="ugpA"
                     /locus_tag="OO596_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6716370.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            55164..56009
                     /gene="ugpE"
                     /locus_tag="OO596_000412"
     CDS             55164..56009
                     /gene="ugpE"
                     /locus_tag="OO596_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6716371.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            56011..57081
                     /gene="ugpC"
                     /locus_tag="OO596_000413"
     CDS             56011..57081
                     /gene="ugpC"
                     /locus_tag="OO596_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR6716372.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            57078..57821
                     /gene="ugpQ"
                     /locus_tag="OO596_000414"
     CDS             57078..57821
                     /gene="ugpQ"
                     /locus_tag="OO596_000414"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6716373.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(57808..58248)
                     /locus_tag="OO596_000415"
     CDS             complement(57808..58248)
                     /locus_tag="OO596_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6716374.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            58368..>59841
                     /gene="ggt"
                     /locus_tag="OO596_000416"
     CDS             58368..>59841
                     /gene="ggt"
                     /locus_tag="OO596_000416"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6716375.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT        14594 a        15113 c        16139 g        13995 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa atcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgtgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acccgatata
    16561 tatgttcact ttaaactcct caggtatccc tgctaataat gaaatgtgca ttaatgataa
    16621 tttcagcctg ttaatataac gacgagatat cattaaagac aaatgcatag cataactata
    16681 gcggggttta ctgtgccgag tgccattttt taccggttaa aaggccatga taaataaata
    16741 tttatcctaa gattaattct tattctgttg ttttttttac aattatcttc cagggatgga
    16801 ttgttttcca ttatttagta aaagaccgga tagtctcggt gcttaaaatc atcgaacata
    16861 taagtattat tgctttgtat catcgtaaat atgtctttca gacatgtatt gtttattttg
    16921 ttactaacaa gtaaaaatat tacaggttat ttatgaaagt atttcacaat atatttaagc
    16981 acttctcttc aaaccatcaa gataagcatt cagataaagt taacagtcat caacaccatg
    17041 gcaaggttga taaaaaatat cgatcaaaca cagttgaagt taatatgctg aatgactttt
    17101 cgcaaataga taacgattta ggcctgaata tcatctattt cctgcaacat ggtcactgga
    17161 gggtaaatga acatagtctt cagatggaga aaatatggtt ttacaatagt gagccatcta
    17221 tagatattca agaatataat agtttcgccg ataatactac taacacgttt atctttacaa
    17281 ttataccaga caataaccat gtgataaaat tatcgtctcc cattacagtt aagattgagt
    17341 gtaaggatgg ttattatttt attaatttct ccggagataa atcagacata atttataaat
    17401 cagacataac ttataaagtt aatgggcttt ctatcatacc tgaaaatttc tttactcttc
    17461 ttagcggtaa ttttaaagct gactggcgtt gggatgtctc taaagagacg tttcctaagg
    17521 ataagtttga tagctatgtt aaatctgtgt tttctaaaat agacttttat aaacaatgtg
    17581 gtgtgattaa cccacaaaat gccaacacgg cttattttgg cgatacagat ggaagagtag
    17641 gcgctgtact ttatgcattg ctcgtttcag ggcatatagg catcagggaa aagggttgga
    17701 gtttattatg tgaattatta aaacacgaaa acatggcttc atctgcatac atacataaaa
    17761 ataataaatt gctctacaat ttattaaata cgagagacat gatattgaat gagttgcatc
    17821 aacatgtttt tttaaaggac gatgcgataa ccccttgtat cttccttggg gatcatacag
    17881 gcgatagatt tagtactatt tttggtgata aatatatcct tactttgcta aattctatgc
    17941 gaaatatgga tggtaataaa gatagccgta ttaataaaaa tgtcatcgtt ctggctggaa
    18001 atcatgaaat caattttaat ggtaactata ctgcacgact agaaaattat aaactctctg
    18061 ccggtgatac gtataactta attaaaacgc tcgatgtttg tcattatgac tcagaaacga
    18121 aagttttaac cagtcatcac ggaataataa gaaatgaaga aaatgaatgt tattgtctcg
    18181 gtgctttgca ggttccattt aatcagatga aagacccaat tgatcctgag gaattagcaa
    18241 atatatttaa taaaaaacat aaacaacaca tggatgatcg tcttatccat ctaattagaa
    18301 gcaatgctgt agcatccaca cctgtatatg aaaattattt taataacact actgctttca
    18361 gaccaaaacc tgaagatatc ttcaaatgtg gacagacgct caaaaaaact aaacagaaat
    18421 atggccatta tggattaggg gtagatcagc atcaaaaaat tgataattat accatggggc
    18481 taaattcctg gaagatagca cctaacgaac gtggtgataa aaaaggtgtc ccagggctta
    18541 gttgttttca acctcaataa aacttcatga gtgctgtggt atctcttttt aattttgcca
    18601 gtatttcata ggttttctta atcgattgta gaggcatgtt ctgatgacgc ctctacaatc
    18661 tttagggact ttctctgcta tgagataaga aatttcatat tatgctttaa ggctgaatga
    18721 tttgtgcaaa atataattat tttcccatcg aacatgaggt gtatgtcata agattaaatg
    18781 ttaactatgt attcctcgcg gtaaataata tgaggtaaaa gatgaaaatt ggaactgtgg
    18841 caggcactaa cgacagtaca acgacaatca caacaaatga tatggtgcaa gagcatgtta
    18901 ccaacttcac taaagaactg tttgggtata ttgcaaatgg tataggtgat gatatatcaa
    18961 gtattgccag gacaatgctc ggtgaggttg tggaaaaaat agatgattgg caaatagaac
    19021 ggttccaaca atctattcaa gatgacaaaa tttcatttac catacaaacg aatcattcag
    19081 aaaaatatag tatgctatcg ggcatgcgcg ctcatattct acgtaggaat aactgttatc
    19141 agtttattgt gacaattaat agtaaaaatt acggttgccc tcttgataat accgatatta
    19201 attggtgcag cattgtttat ttacttaata atatgacagt aaatgataat gctaatgatg
    19261 ttgcagttac tgagtcgtac aaacccatct ggaactggaa aatatcgcaa tacaatgtat
    19321 ttgatattaa attcgaaaca atgataaaac cccagttcgc tgacagaacc tatttctcca
    19381 attgttcacc tgttgatccc acaagtacca gaccgaccta ttttggcgat accgatggct
    19441 cagtcggtgc agttttgtac gcgctttttg caacgggaca tttgggaatc atggctgaag
    19501 gagaaaattt ccttagccag ttgcttaata tagaagatga agttctcaac gtattgctaa
    19561 gggagaactt taatgagcaa cttgacacta atgttaatat aataatcagt atcctgaaca
    19621 gaagagacat tgttttagaa agtcttcagc cttatttggt tattaataaa gacgcagtca
    19681 cgccatgtac gtttctgggc gaccagactg gcgatcgatt tagtaatatc tgtggcgata
    19741 aatttatcat cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt catgtattag
    19801 ctggaaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg cgcatgaaac
    19861 cacttgatga ggatacctac tctgggataa aagattatcc tgtatgtttc tatgacccca
    19921 aatacaaaat aatggcaaat catcatggga taacttttga tgatcagcga aaacgttaca
    19981 ttataggacc gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat
    20041 tagcggcaat tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata
    20101 gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcgaca gattacagac
    20161 ctaaacttga agcactatta gcatgctcac aaatgttagg tataaatcaa gttgttgcac
    20221 ataacggtaa tggtggaaga gaatgtatag gtgaaacggg gacagtttta ggtcttaacg
    20281 cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg
    20341 gtgcagggcc tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg
    20401 gacttatgcc tttgatcaga aggcgcacaa tgttacagct ctgaaccata gacaacatga
    20461 atattaatgt tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc
    20521 ctgcaactga acaggcgtga aacgctatcc aacaggatgg ataccgtttt gtcagccagt
    20581 tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga
    20641 caacattact acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg
    20701 gtcgtatttg tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg
    20761 gcagttgtaa cacgcgtcgt ttgcttgcag ggattatggc gggtcgatta tggataagag
    20821 taagcgccat ctggcgtggt gggttgtcgg gttactggtg gtggctgccg tcgtggcgtg
    20881 gtggctgttg cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg ggcgcattga
    20941 agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa
    21001 agaaggccag tttgttcgcg aaggcgaagt gctggcgaag atggatactc gcgtgttgca
    21061 ggagcagcga ctggaagcca tcgcgcaaat caaagaagca caaagtgccg ttgctgccgc
    21121 gcaggctttg ctggagcaac ggcaaagcga aactcgtgcc gcgcagtcgc tggttaatca
    21181 acgccagtcc gaactggact ccgtggctaa acgtcatacg cgttcccgct cgctcgccca
    21241 acgaggggct atttctgcgc aacagctgga tgacgatcgc gccgccgctg agagcgcccg
    21301 agctgcgctg gaatcggcaa aagctcaggt atcggcttct aaagcggcta tagaagcggc
    21361 ccgtaccaat atcattcagg cccaaactcg cgtcgaagcc gcgcaagcca ctgaacggcg
    21421 tattgccgca gatatcgatg acagtgaact gaaagccccg cgtgacggac gcgtgcagta
    21481 tcgggttgct gagccagggg aagtgctggc ggcaggcggt cgggtgctga atatggtcga
    21541 tctcagcgac gtctatatga ctttcttcct gccaaccgaa caggcgggca cgctgaaact
    21601 tggcggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat
    21661 cagttttgtc gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg
    21721 gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct
    21781 ggaatatgtc aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg aagaactttc
    21841 gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc
    21901 ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaacgt tgcgctgaac
    21961 aatattactc tcgatattcc ggcccgctgt atggtcgggc tgattggtcc ggatggcgtc
    22021 gggaagtcga gcttattgtc gttgatttcc ggtgcccgcg tcattgagca gggcaatgtg
    22081 atggtgctgg gcggcgatat gcgcgacccg aggcatcgcc gcgacgtctg cccgcgcatc
    22141 gcctggatgc cgcaggggct gggcaaaaac ctctaccaca ccttgtcggt gtatgaaaac
    22201 gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat
    22261 gagctgctga ccagcacggg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc
    22321 ggcgggatga agcaaaaact tgggctgtgc tgcgcgttaa tccacgaccc ggaactgttg
    22381 atccttgatg agccaacaac gggggttgac ccgctctccc gctcccagtt ctgggatctg
    22441 atcgacagta ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac cgcctatatg
    22501 gaagaggccg aacgcttcga ctggctggta gcgatgaatg ccggagaagt gctggcaact
    22561 ggcagcgccg aagagctacg gcagcaaacg caaagcgcta cgctggaaga agcatttata
    22621 aatctgttac cgcaagcgca acgccaggcg catcaggcgg tagtgatccc accgtatcaa
    22681 cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc
    22741 gttgccgttg atcacgttaa tttccgcatt ccacgcgggg agatttttgg ttttcttggt
    22801 tcgaacggct gcggtaaatc caccaccatg aaaatgctca ccggactgct gcccgccagc
    22861 gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt
    22921 cgggtgggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac
    22981 cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc aagagtggct
    23041 gaaatgagcg agcgttttaa gctcaacgac gttgaagatg ttctgccgga gtcattgccg
    23101 ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta
    23161 atcctcgatg agcctacttc tggtgtcgat ccggtggcga gggatatgtt ctggcagttg
    23221 atggtcgatc tctcgcgcca ggacaaagtg actatcttca tctctaccca ctttatgaac
    23281 gaagcggaac gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt
    23341 acaccgcagg aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc
    23401 tatttgcagg aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac
    23461 accacccacg cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc
    23521 gaagcgctgg aactgcgacg cgatccagta cgttcgacgc tggcgctgat gggaacggtg
    23581 atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg
    23641 gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga cactcaacct ctccggttcc
    23701 cgttacttta tcgaacagcc gccgctcact agttatgacg agcttgatcg tcggatgcgt
    23761 gcgggcgata tcacggtggc gattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt
    23821 ggtacgcctg tggaactcgg cgtctggatc gacggagcga tgcccagtcg tgccgaaacg
    23881 gtaaaaggtt acgtgcaggc aatgcaccag agttggcttc aggatgtggc gagccgacaa
    23941 tcgacacccg ccagccaaag cgggctgatg aacattgaga cgcgctatcg ctataacccg
    24001 gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc cgcttctgct gatgatgatc
    24061 ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agctagggtc gattatcaac
    24121 ctttacgtga cccccaccac ccgtagcgaa tttttgcttg gcaaacagct gccgtacatc
    24181 gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta
    24241 ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcattgcc
    24301 accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga
    24361 acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct
    24421 tcgctggaag ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact
    24481 atcgcccgcg gaacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc
    24541 ccgttgctga tagccatccc gctggtgatg ggcttaagca tcctgctgct gaaaaaacag
    24601 gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc
    24661 tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt
    24721 cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg
    24781 atcaatcgca gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac
    24841 cggagatgat caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg
    24901 cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccggatattc
    24961 aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga
    25021 atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt
    25081 tggtatcgct ggaaacccgg atgcgcttta acccgaacct cgatcccgcg tggtttggcg
    25141 gggtgatggc gatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc
    25201 tgatccgcga gcgtgaacac ggcacggtgg aacacttact ggtgatgccg ataacgccgt
    25261 ttgagatcat gatggcgaag atctggtcga tggggctggt ggtgctggtg gtatcgggat
    25321 tatcgctggt gctgatggcg aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc
    25381 tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg
    25441 ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctgccgc
    25501 tgcaaatgct ttccggtgga tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca
    25561 ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatt ctctaccggg
    25621 gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt
    25681 ttttcaccat tgcgctgctg cgattcagga agacgattgg gacaatggcg taaggaaggg
    25741 aaacttgact tcatactgtg atgtcatact attgatgtca taaagtgaag tcataaggac
    25801 atgccatgag aaccaaggta caggctttac ggaagaaaaa aaaatacatt ggatcaaata
    25861 tttaaaactc ctgttcctca agggatcaag tggtctgata tagagtcact ggttaaagca
    25921 ttaggcggag aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc
    25981 gttgcgtgtt tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc
    26041 gtgcgtgact ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact
    26101 ctatggagat tgccgggcaa ccagctgtta ttacctacgt gccagaactg aatgcatttc
    26161 gtggcaaatt cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc
    26221 tgcaaaaaga aggggaactt tccctgcgag agtatcttga ggattgtaaa gctgcgggta
    26281 ttgagccgta tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc
    26341 tgagtgaacg ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta
    26401 ttgaaacact gaatgagcgt ttgaatcatt tataaatttt agcggcgtac attacgcaga
    26461 atatctgcct ttacaggatc tatctatgca actgaactta attttataaa ttaagttcag
    26521 ttaatatctt ttctttcctc gtttcagaaa taactcaatc ttccttcggc aagcactgca
    26581 aatgcccatg ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcgc
    26641 ccatatcacc tttcagcacg gcgatttcca gacagtcgtc gtgattgata tgcacatgca
    26701 gcgtggcgac ggagagatcg tggtgatggt gctgggtgga gacaatgcgg ctggctaagt
    26761 cgcgtttttc gtgttcatac acataggaga gcaccgcgaa accttgcgtg ccgtgctgct
    26821 gagtggcctc ctgggccagg gcgctacgca gaatatcgcg gatagcttcg gaacggttgt
    26881 tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca tcaagcgtga
    26941 tggtgactcg ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca cggggaatgc
    27001 gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc
    27061 caccacctgg gtttcgacga tttgtccgtt gtccatcacc atcacccgct gacagaagcg
    27121 ttccaccagg cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact gttgttgtag
    27181 ctttttcagc aggcgaatga cgcccgcctg taacacgaga tcaaggttag aaacggcttc
    27241 atccagaatc agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag
    27301 ctggccgccg cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc
    27361 cttcagcatt tcgctggcgc gcgccagttg ttcggatttt ttcagtgata gcaggtggcg
    27421 catcggttca cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc
    27481 ctgaaatacc atctgaatat cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc
    27541 cagcggttcg ccacgccagc taatattccc ctgcgcgggc gattctaaac ccaccagcaa
    27601 ccgcgcgagg gtacttttcc cgcagccgct gcgccccagc agggcgacgg tttcgccgct
    27661 tttcagggtc agggaaacgt tattcagcac cgcctgatgt tgatgttttc cgttaaatcc
    27721 accgtgggca tagtgatggg aaaggccgga gacgttaagt aaagtcatga tgccagctcc
    27781 ataccgtaga gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg tttgggggcg
    27841 ttaaacagcg tttctacatc gccctgttcg acaattttac cttgtgacat caccgccacg
    27901 tcatccgcca gacgcgccac aacgcccata tcatgggtga ccagcagcat tcccggcgct
    27961 tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg
    28021 tcggtggtcg gttcatcggc gatgataaac ggtgattcac acagcaccgc catcgcaatc
    28081 atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg
    28141 gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc
    28201 cctaacgcca ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg
    28261 ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca gggataaacc
    28321 gctttgccat cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc
    28381 agcgtcgcgg cgcaggttaa tgatttcccg ctgccgctac cgccgactaa cgccagcacg
    28441 cgcccgcgtt gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg
    28501 atattacgta gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat
    28561 ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg ataaacagcg
    28621 ccagccccgg ccagaacatt tgcagcggct gggtccagat atactggcgc gcgtcgttaa
    28681 tcatcacgcc ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg
    28741 cgacgtgcag catcatatgg ccgatatcca gcgttgccag caccagcagc gaagggatca
    28801 ccgcgcctgc cagatgatcg acaaacaccc gcacatggcc cgcaccggaa agccgtgacg
    28861 ccagcacaaa ctcgcgttgg cgtagtgaaa tcaccaagct gcgcaccatg cgtgcatacc
    28921 acgcccagtg cgacagggcg atggcgataa ttacgttggt cagcccggtg ccgagcacgc
    28981 caaccataaa gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca
    29041 tggtggcctg atcaacgcgc ccgccaatca acccggcgct gccgccaata accagcccta
    29101 atgtcagcac cagcagcagg caggccatta ccgaaccgag cgacacgcgg gtcgctgcca
    29161 tcagccgcga gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc tgcgcatccg
    29221 gcgaaagcag gcgcgacggc aaatcaatcg cctgggggtc atacggcagc caccactggc
    29281 tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc
    29341 gggaagagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag
    29401 cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag
    29461 cgtaaagcac tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc
    29521 gacgcccggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca gttcgccgat
    29581 gtgcatcccc acggcggtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc
    29641 ggtctgtttg tcgttcaggc cgcgcagacg cgcccaagtg acgtgacgct gaccggcgac
    29701 gtccagcata ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa aggcaatgga
    29761 aaccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta gccattgcag
    29821 atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca tcgacacgcc
    29881 aagaaacgcg atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc gcgccgccca
    29941 gataccgagc ggtacggaag tgagcagaat taataccagc gccgcacctg caagttccag
    30001 cgtggcgggc aggaagttca gcatatcgtc cagcaccggg cgttggctgg cgaatgagat
    30061 accaaagtca agatgcagcg ccttccacaa ccaggtaccg tactggacgt acagcggctg
    30121 atccagcccg agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga
    30181 taaacgcaga taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat
    30241 caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa
    30301 cattaaggtt tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag
    30361 gggatattac ccagctccgg ttttgatacc accatcatcg agatgtagct gataggcaga
    30421 taaaccgcct cgtcatgcag acgggtcaaa atgtcgcgat acagcgcctg acgttgcgtt
    30481 tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggctt gtcggctaat
    30541 ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt
    30601 ggatcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga
    30661 cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc
    30721 atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgac
    30781 agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc
    30841 caaccggctt tttccagcaa ctctttcgct ttctgcggat cgtactggcg cggtttcagg
    30901 ccgagattag cgtaaggcac agaaggggca aacagggtgt cggcgacctg cggggtgcca
    30961 tataacgcgt tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact
    31021 gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc
    31081 tgtgacagtt gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt
    31141 aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg
    31201 gtagtcgggt ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag
    31261 tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac
    31321 ggtccggtgc caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag
    31381 ggagcgataa aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg
    31441 cttttcaggg taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt
    31501 gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc
    31561 gccgcctcgg catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag
    31621 gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa
    31681 ccgtctgcct gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta
    31741 ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt agtgatttca
    31801 tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg
    31861 cggagtgtgg agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga
    31921 cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt
    31981 gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg
    32041 tgggggtgca gatcgcaagg cttaaatttt ctagctgaca atggctgact gacagcgagg
    32101 agtgataggt gctatctacg ctgacgattt gccaggcgct gccgccacgc tgtttaacga
    32161 tggcttcttt gcgcgtccag atgcgccaga acatttccag ttgctgatca ggatgcacgg
    32221 cgtccatttc agcgtgttcc ccgaggctga acacggcgtt cgccagccag cgccagttgg
    32281 cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc aacagcagag
    32341 cgatatcgtc accgctatgg cttaagttga accatagcgg cgtttccggc gcgaatgcag
    32401 gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg
    32461 cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg
    32521 gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag aactatccga tacatatcag
    32581 ggccaacgtt taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct catcccggta
    32641 accgactttt cggtctgcct ggccccagta aaatcgccag tttgctaccg cctttggtgg
    32701 tttccatcca gattttacac acactggtta atggcaccga aagtagcatc cctaccgggc
    32761 cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac atccccaggc
    32821 gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc actaaaaaca
    32881 atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg
    32941 gtacggcgga aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac gccagtactg
    33001 cccacatcag cgcaaactgc acccccatca gctccagccc cagccagacg atgacacctg
    33061 tccataaact gagtagcgtc ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt
    33121 gtaatcctgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt
    33181 ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg
    33241 ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc
    33301 gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt
    33361 taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata aattcgttaa
    33421 acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc accactacaa
    33481 tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtaacg agcgggttga
    33541 ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa
    33601 tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg cccgttttat
    33661 cgggttgagg gatttccatt ggggcttcct tgtgactttt tgtattaagt gtagcgggag
    33721 tcacgccagc aatattctga ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg
    33781 aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta
    33841 aacggattgc gcctgaattt gcgcattgtc tccattgtca tgtttaactt cgccagctac
    33901 ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt
    33961 agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgt
    34021 cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt cttcggttta
    34081 tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc cagtctgccc
    34141 gtcatcagcc tgttattact ttgcctggga cgcgtgatcc ttgggattgg gcaaagtttt
    34201 gccggaacgg gatcgaccct gtggggcgtt ggcgtggttg gctcgctgca tatcgggcga
    34261 gtgatttcgt ggaacggcat tgtcaattac ggggcgatgg cgatgggtgc gccgttaggc
    34321 gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taatcattat gggcgtggcg
    34381 ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaacca
    34441 ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcact ggcactggct
    34501 tctgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgc taaaggttgg
    34561 gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta
    34621 ttccctaacg gcattaaccg tatcggcggc ttaaacgtgg cgatgatttg ctttagcgtt
    34681 gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaagtcggc
    34741 gtcttactgg cgggggcagg gttttcgctg gtgttcccgg cattgggcgt agtggcggta
    34801 aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt atttatggat
    34861 ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagctgggc gggcgtaccg
    34921 gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta
    34981 aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa cttactgaat
    35041 aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat cgtcagctgg
    35101 cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta
    35161 cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag
    35221 ctcaatggct ttgttggtta ccacttgcag ctgcggatcg cggctacgtt gctgatcttc
    35281 cagacgcttc gccaacaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac
    35341 tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt
    35401 accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt
    35461 gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc
    35521 tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag
    35581 attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat
    35641 cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc
    35701 cagtcttttc aacagaatat tgagtaatac gccatacatt ggcaggaaga aaacgatact
    35761 gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa
    35821 ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc
    35881 gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa
    35941 aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg tggcgatacg
    36001 ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccaggaac ccatatagaa
    36061 tagcgacgag atgacgtagg agatcagcaa cgccgggatc atcaccgcaa agatgatacg
    36121 acgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg
    36181 gaagctaaac gcgccccagg tggtatggaa acccaaaatg gagacgggaa gctgaaccag
    36241 atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca acgccttata
    36301 gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg
    36361 gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc
    36421 gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt
    36481 taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt tatgtgatga
    36541 gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac ttggcctgcg
    36601 ctgcccggaa cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc ctggcgaaac
    36661 gttgctgatt atcgccgacg atccggccac tacccgcgat attcctgggt tttgtacctt
    36721 tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt atttgattcg
    36781 taaaggcggt tgatggggaa agattggctt cgatgccgcc ttttcccctc accctaaccc
    36841 tctccccaga ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct
    36901 cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg tatctgccag
    36961 caccgccagc cacaggccgg tcatccctaa cagcgtggtg acgaggaaga tccctttcag
    37021 ccccagcgca atggtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat
    37081 catttgcacc aggccgcgca ggtggttatg ggttaatgct gcatcagcgg tttccagcgc
    37141 cacgtctgtg ccactgccca ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc
    37201 gttaatacca tcaccgacca tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt
    37261 gactttatct tccggcaata ggcccgcttt aaactccagc cccagctccc cggcgattgc
    37321 cgccgctgca cgtggattat cgccggtgag gatcactcct ttgaccccca gcgcgttcag
    37381 ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa tgatacccag
    37441 cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca gttcattaat
    37501 caaaccagca aatgcatcag cgggatgttt cccggcagcg caaatcaata cgcgctcacc
    37561 gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg cggtgggaat
    37621 ggcgagttca gcaacctgtg cttcgcgtac gatggcttgc gccagtggat gcgtcgcgcc
    37681 ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg
    37741 aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacctg
    37801 agtaacacga cctagttgtt ccagcgccgc tccgccttta atcaacgccc cacgacgcgc
    37861 tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg cacacgggca
    37921 gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg cggcaaacag
    37981 cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg tatagatacg
    38041 gctgaaacgg tcgataaacc gctcaatggg ggcgcgacgc tcttcggctt cttcgatcag
    38101 tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag
    38161 acggtctacg ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat
    38221 ggattctccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca gtttaccgtc
    38281 ggcaggcaaa cgcccacctg cggcgacttc aatcacatcg ccaggccgca ggctgttaat
    38341 cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat
    38401 taacgcgcta acgccctgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat
    38461 caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat
    38521 agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga tcagccgtaa
    38581 tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg ccagttgccc
    38641 gaacggatga ttgaactgct ccagacccca gctgattgcc atcatcacga ttagtgtaat
    38701 cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg
    38761 cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacgaatgt cattgtcggc
    38821 atcaaccacc agtttttcgg tggcgaacaa cacctgcacc tgattcacac ctgcaagctg
    38881 gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccac tgactttcca
    38941 gctatagcgg gtgccggaga cgttttcaga gagcgttggc gagctggagc atgcgccgtc
    39001 gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag caaattgagg
    39061 ggctttcttg ccgtgattgt caggtgtcga catggcatac tccggttaag ttttttctca
    39121 ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagtg
    39181 agcggatgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac
    39241 ggaagcgtcg gcgatagtgg ctccccagcc agacaaagtt actgataaac agcaacgaag
    39301 cgccaacaaa cgcagagagc gccggagcgg tcggacggaa gaaccacagt tcacctgcca
    39361 gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga tactcttcca
    39421 gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc agtggcagag
    39481 gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga
    39541 ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc
    39601 ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg aacatcggcg
    39661 cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc cagcgttgcc
    39721 aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa
    39781 acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg
    39841 tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat
    39901 gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct tttctttatt
    39961 gttatcattg gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa
    40021 aaagctgata atgatatggc tccgctccag caaaagctgg tggtggtgag caacaagcgg
    40081 gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt
    40141 atgcgctacg aggcaagctt caaaccgcaa agcggaggaa tggagcagac gtttcgcctc
    40201 gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga
    40261 acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc
    40321 tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gtaccttttc ggcggtggtc
    40381 atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagctgcat accatgcatc
    40441 agcacggtaa aaatcagcgc cacgttaacg aaaatattta gcgggcgcgg gaagggatgc
    40501 accagattga ggattaaaaa tccccaaacg cagagcatta acaaacgacc aatattaatc
    40561 agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct gacccgccac
    40621 tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt cgacttcgct
    40681 ttcgacataa atcagggctt cgtcagccag ccagccgtta tcttccagta aatttatcgt
    40741 ctcttctaac aagccacggc ggaacggtgg atcgacaaac acgatattat gcggtgtgcc
    40801 tttttgcgcc aggaatgaca tcgcgttgct gttcaccacg cgtgcattgc ctgcttttag
    40861 tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa ttaacgtggc
    40921 ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc
    40981 cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt cgcgtacgcg
    41041 gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc ctcgccactg
    41101 cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg
    41161 ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga
    41221 ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac
    41281 agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg
    41341 gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaccgg
    41401 ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttcggaacaa gccgttgaag
    41461 agcagccgca ggcgcatact gaagccgagg cggaaacttt tgctgccgac gttgtggaag
    41521 tcactgaaca ggttgttgaa agtgaaaaag cgcagcctga agcggaagtt gttgcacagc
    41581 cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt
    41641 tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa
    41701 ccgtagagat tgtcaaagcg gcggaagaag aagcggctaa agaagaaatc accgacgaag
    41761 agctggaagc acaggcgctg gcagccgaag cagcagaaga ggcggtgatg gtggttcctc
    41821 cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa ccaaccaaag
    41881 aaggtttctt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg
    41941 gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag
    42001 agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga
    42061 cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag
    42121 aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc
    42181 cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc
    42241 tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc
    42301 gtgcagcagc ggttgaacag cttcaggtct ggggtcagcg caacaatatt ccggtgattg
    42361 cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag gcagctaaag
    42421 cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac aaatcgcacc
    42481 tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc
    42541 atgaagttat gctgactatt gatgccagca ccgggcagaa cgcggtaagc caggccaaac
    42601 tgttccacga agctgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga
    42661 aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg
    42721 tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt
    42781 ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg
    42841 gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac
    42901 cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc
    42961 cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt
    43021 tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac tgatggaccg
    43081 tactgtctac gataacgtgg cgatcccgct gattatcgct ggtgccagcg gtgacgatat
    43141 tcgtcgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt
    43201 ccctattcag ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa
    43261 taagcccgcg gtactgctgg cggacgaacc gaccggtaac ctggacgacg cgttgtcgga
    43321 agggattcta cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac
    43381 gcacgacatc aacctgattt cacggcgttc ccatcgcatg ctcaccctga gcgatggtca
    43441 cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg
    43501 gtgggcgtct ggatcgcttc cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg
    43561 caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg
    43621 aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca
    43681 cgtttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg
    43741 tgtacaaaaa cgttaatcag gcggcgacgc agtattatcc gtcaccgcaa atcactgttt
    43801 acttgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg
    43861 agcaaggcgt ggagaaagtg aactatcttt ctcgtgaaga cgcactgggc gagttccgta
    43921 actggtctgg ttttggtggt gcgctggata tgctggaaga aaacccgctt ccggcagtgg
    43981 cggtggtgat cccgaaactc gattttcagg ggacggaatc actcaatacg ctgcgtgatc
    44041 gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc
    44101 tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg ttgatggtgg
    44161 cggcagtgtt cctcgtcatc ggcaacagcg tacgtctgag catctttgct cgccgtgact
    44221 ccatcaacgt gcagaaactg attggtgcga cggatggttt catccttcgc ccgttcctgt
    44281 atggtggcgc actgctggga ttttctggcg cattgttgtc attaattttg tcagaaattc
    44341 tggtgctgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga acgaagtttg
    44401 atatcaatga tttatcgttc gatgaatgcc tgctattgct gctggtatgc tcgatgattg
    44461 gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat
    44521 aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga gatcccgttg
    44581 tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcttgc
    44641 attgaacttg tggataaaat cactgtctga taaaacagtg gatgataacc tcgttgctct
    44701 taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt
    44761 tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac
    44821 atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa
    44881 aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg
    44941 tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt
    45001 caggaaggta acatcggcct gatgaaagca gtgcgccgtt tcaacccgga agtgggtgtg
    45061 cgcctggtct ccttcgccgt tcactggatc aaagcagaga tccacgaata cgttctgcgt
    45121 aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg
    45181 cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt
    45241 gaactgggcg taaccagcaa agacgttcgt gagatggaat cacgtatggc ggcacaggac
    45301 atgacctttg acctgtcttc cgacgacgat tccgacagcc agccgatggc accggtgctc
    45361 tatctgcagg ataaatcatc taactttgcc gacggcatcg aagatgataa ctgggaagag
    45421 caggcggcaa accgtctgac cgacgcgatg caaggtctgg acgaacgcag ccaggacatc
    45481 atccgcgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt
    45541 tacggtgttt ccgctgaacg tgtacgccag ctggaaaaga acgcgatgaa aaaactgcgc
    45601 gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg agtccggggt
    45661 ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc caatccccac
    45721 gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc gggtatgttt
    45781 tagcagagta tgctgctaaa gcacgggtag ccatgcataa aacgaaataa agtgctgaac
    45841 aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat gaacataaag
    45901 ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa
    45961 gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta cggtgaccaa
    46021 gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc
    46081 aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg
    46141 gcgaacaaag tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca
    46201 acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac cccagcggca
    46261 accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac
    46321 tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt
    46381 gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt gcaggacggc
    46441 ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat
    46501 ttctctacgc tggtggcgcg tctgaagaaa gagaatatcg acttcgttta ctacggcggt
    46561 taccacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactcag
    46621 tttatggggc cggaaggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg
    46681 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcaaa caaacccatt
    46741 gttgacgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg gaccacctac
    46801 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc
    46861 aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacgtg ggatgagaaa
    46921 ggcgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg cacggcgaca
    46981 gatgcgaagt aatcattaat cggcaacttt gggttgccgc caaattgcta atatcgagta
    47041 cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctataagat ccaaagaaat
    47101 cagtaaattg caacacacat tgtaggcctg ataagcgtag cgcatcaggc aatttctcgt
    47161 tggtcatcac ttttgaaatc aacttgacga ttaacgcttc tcccagccgc cctgttgcgc
    47221 cgtaaatccc agtgcctgca taaacgctgt catcacaccg cgatcttcca cgcctgcatc
    47281 cgccatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg
    47341 ccccacaccg cgacgtcggg tgacttcccg cacacgcagg gaatccagtg ctccctcggt
    47401 gccgcttaag gttacccgta cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat
    47461 acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag
    47521 gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa
    47581 cccgtgtaaa tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg aggctttctc
    47641 ttttttattt cttttggcag gtgattaatt ttttaacagc aataattaca aagttaaaac
    47701 attagagaat gaaaaatacc cagcataatc ccctgaatgg tagtgaatta ttccgccctt
    47761 tgtgccgtta ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag
    47821 aatattatct gttcttaatc gactgaaaaa tagggatttt aatcactgtt atcacaaaat
    47881 actgcgctaa acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct
    47941 gatttgttaa cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg
    48001 aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct
    48061 atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg
    48121 ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga
    48181 attaagggcg acaagctggt tggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc
    48241 gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat acgttattgg tcatctgtgt
    48301 tcttcttcta cccaacctgc atcagatatc tacgaagacg aaggtattct gatgatctcg
    48361 ccgggagcga ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc
    48421 gggctggact cttcccaggg gccaacggcg gcaaaataca ttcttgagac ggtgaagccc
    48481 cagcgcatcg ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg
    48541 caggacgggc tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg
    48601 gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac
    48661 tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg
    48721 aaaactcagt ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgctggc
    48781 gatgctgccg aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac
    48841 cagggcatcg ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg
    48901 atcacctacg cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag
    48961 ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg
    49021 aactgggatg aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc
    49081 gatggttcat ccacggcagc caagtgatca tcccaccgcc cgtgaaatgc gggcgggttt
    49141 agaaaggtta ccttatgtct gagcagtttt tatatttctt gcagcagatg tttaacggcg
    49201 tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta
    49261 tcggcatgat caacttcgcc cacggcgagg tttacatgat tggtagctat gtctccttta
    49321 tgatcatcgc cgcactgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg
    49381 gattcgtcgg cgcaatcgtc attgccagcg cctacggctg gagtatcgaa cgggtagcct
    49441 atcgcccggt gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca
    49501 tcttcctgca aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg ctgcccagtc
    49561 tgtttaacgg tcagtgggtg gtggggcata gcgaaaactt ctctgcctct attaccacca
    49621 tgcaggcagt gatctggatt gttactttcc tcgccatgct ggcgctgacg attttcattc
    49681 gttattcccg tatggggcgc gcctgccgtg cctgcgcgga agatctgaaa atggcgagcc
    49741 tgcttggcat caacaccgac cgggtgattg ctttgacctt tgttattggc gcggcgatgg
    49801 cggcagtggc tggcgtactg ctcggtcagt tctacggcgt cattaacccc tatatcggct
    49861 ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc agcattccag
    49921 gggcgatgat tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga
    49981 gtacggaata taaagatgtg gtctcattcg ccctgctgat tctggtgctg ctggtgatgc
    50041 cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat
    50101 ggcactgctc tctgccgcga tgttctttgt cctcgctggc gtctttatgg gcgtgcaact
    50161 ggagctggat ggcaccaaac tggtggtcga cagcgcttcg gatgtccgtt ggcagtgggt
    50221 gtttatcggc acggcggtgg tctttttctt ccagcttttg cgacctgctt tccagaaagg
    50281 gctaaaaagc gtttccgggc cgaagtttat tctgcccgcg attgatggct ccacggtgaa
    50341 gcagaaactg ttcctcgtgg cgctgctggt gcttgcggtg tcgtggccgt ttatggtgtc
    50401 acgtgggacg gtggatatcg ccactctgac catgatctac attatcctcg gtctcgggct
    50461 gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg tacggcggtt tttacgccat
    50521 cggcgcttac acttttgcgc tgctcaatca ctattacggt ctggggttct ggacctgttt
    50581 gccgattgcc ggactgatgg cagcggcggc gggcttcctg cttggttttc cggtgttgcg
    50641 tctgcgcggt gattatctgg cgatcgttac cctcggtttc ggcgaaattg tgcgcatatt
    50701 gctgctcaat aacaccgaaa ttaccggcgg cccgaacggt atcagtcaga tcccgaaacc
    50761 gactttcttc ggccttgagt tcagccgtac cgcccgtgaa ggcggttggg acacgttcag
    50821 taatttcttt ggcctgaaat acgatccctc cgatcgcgtc atcttcctgt atctggtggc
    50881 gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg
    50941 gcgtgcgtgg gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg gcttaagccc
    51001 gcgtcgtatc aagctgactg cctttaccat cagtgccgcg tttgccggtt ttgccggaac
    51061 gctgtttgct gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc
    51121 gtttgtactg gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc
    51181 ggcaattttg ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt
    51241 aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat
    51301 gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag
    51361 ccattattat ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc ggtgaacaac
    51421 gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga
    51481 aaaaccacgg tttttaactg tctgaccgga ttctacaaac ccaccggcgg caccatttta
    51541 ctgcgcaatc agcacctgga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg
    51601 cgcaccttcc agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa cctgctggtg
    51661 gcgcagcatc aacaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc
    51721 cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg
    51781 ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag
    51841 attgcccgct gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt
    51901 cttaacccaa aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac
    51961 aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga
    52021 atttatgtgg tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat
    52081 aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc
    52141 ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcctgca
    52201 tatcaatcag ggcgagattg tcaccctcat tggcgcgaac ggggcgggga aaaccacctt
    52261 gctcggcacg ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt ttgatgataa
    52321 agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga
    52381 agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttttt
    52441 tgccgaacgc gaccagttcc aggagcgcat aaagtgggtg tatgaactgt tcccacgtct
    52501 gcatgagcgc cgtgttcagc gggcaggcac catgtccggc ggtgagcaac aaatgctggc
    52561 gattggtcgc gcgctgatga gcaacccgcg tttgctgcta cttgatgagc catcgcttgg
    52621 tcttgcgccg attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg
    52681 gatgactatc ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg
    52741 ctacgtgctg gaaaacggcc atgtggtgct ctccgatact ggtgatgcac tactggcgaa
    52801 tgaagcggtg agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa
    52861 aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc
    52921 ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt
    52981 acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt
    53041 ttccgcttat ccgtgcccca tctcccattt tccctcaccc acgccgtcac cgccttgtca
    53101 tctttctgac accttacttg caggtttatg aagttggcac cgccaccatg atggcatcga
    53161 aagccattaa accggtatat gacgtgttta aagaggcagg gattcaattc gatgagtcgc
    53221 agtttgtgcc gacggtttcc ggttactact ccgacagcaa aacgggccac ttactctccc
    53281 agccattcaa cagctcgact cccgttctct attacaacaa agacgccttc aagaaagcgg
    53341 ggttagaccc ggaacagccg ccgaaaacct ggcaggatct ggcggactct tccgcaaaac
    53401 tgaaagcctc cggtatgaag tgcggctacg ccagcggctg gcagggctgg atccaactgg
    53461 aaaactttag cgcctggaac ggtctgccgt ttgccagcaa aaacaacggc tttgacggca
    53521 cggacgcggt gctggagttc aacaagccgg agcaggtgaa acacattgcc atgctcgaag
    53581 agatgaacaa gaagggcgac ttcagctacg tcggtcgtaa ggatgaatcc accgagaagt
    53641 tctataacgg tgattgcgcg atgaccaccg cctcttccgg ttctctcgct aatattcgtg
    53701 agtacgccaa atttaactat ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg
    53761 caccgcaaaa cgccattatc ggcggggcca gcctgtgggt gatgcagggc aaaaataaag
    53821 aaacgtatac cggcgtggcg aagttcctcg acttcctcgc gaagccagaa aacgctgccg
    53881 agtggcatca gaaaaccggt tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg
    53941 agcagggctt ttatgagaaa aacccagggg cggataccgc gacgcgtcag atgctgaata
    54001 agccgccgtt gccgttcacc aaagggctgc gtctgggcaa catgccgcag atccgcgtga
    54061 ttgtggatga agagctggag agcgtgtgga ccggtaagaa gacaccacag caggcactgg
    54121 ataccgccgt tgagcgtgga aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt
    54181 aatcagtgta atgtcggatg cgtttcgctt atctgacctg gcatcgcgtg taggccggat
    54241 aagcgaagcg catccggcac agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg
    54301 ttccgctcgc gctggctgcc ttatctgctg gtcgcgccgc agctcatcat caccgttatc
    54361 ttttttatct ggcctgcggg cgaagcgttg tggtactcgc ggcaaagcgt cgatccgttt
    54421 ggtttctcca gccagtttgt cggtctggat aacttcgtca cgctgttcca tgacagctac
    54481 tatctagact ccttctggac gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg
    54541 ctggtatcgc tgttctttgc ggcgctggtg gagtacatcg tgcgcggcag ccgtttctat
    54601 caaaccttga tgctgctgcc gtatgccgtg gctcccgccg ttgccgccgt attgtggatc
    54661 ttcctgttta accccggtcg cgggctgatc acccattttc tcgctgagtt cggctacgac
    54721 tggaaccacg cgcaaaacag cggtcaggca atgtttctgg tggtgtttgc ctcggtgtgg
    54781 aagcaaatca gctacaactt tctgttcttc tatgccgcgc tgcactccat tccccgttcg
    54841 ttgattgaag ccgcagctat tgacggcgca gggccgattc gccgcttctt taagattgct
    54901 ctgccgctta tcgctccggt gagtttcttc ctgctggtgg tgaacctggt gtacgccttc
    54961 ttcgacacct tcccggtgat cgacgccgcc acgtccggcg ggccggttca ggccaccact
    55021 acgctgattt ataagatcta ccgcgaaggc tttaccggac tggatctggc ttcgtctgcc
    55081 gcgcagtcgg tggtgttgat gttcctcgtc atcgtgctga cggtggtgca gttccgctat
    55141 gttgaaagca aggtgcgtta ccaatgattg agaaccgtcc gtggctgacg atattcagcc
    55201 ataccatgct gatcctcggg atcgcggtga tcctcttccc gctgtacgtg gcgtttgtcg
    55261 cggcgacgct ggataaacag gccgtctatg ccgcgccgat gacgctcatc cccggcacac
    55321 atctgctgga aaatatccac aacatctggg ttaacggggt aggcacgaat agcgcgccgt
    55381 tctggcggat gttgcttaac agctttgtga tggcgttcag cattacgctc ggcaaaatta
    55441 ccgtctcgat gctctcggca tttgccattg tctggtttcg ttttccgcta cgtaacctct
    55501 tcttctggat gatttttatc accctgatgc tgccggttga agtacgtatc ttcccgacgg
    55561 tggaagtcat cgccaacctg aaaatgctcg acagctacgc cggtttaacg ctgccgctga
    55621 tggcctcggc gaccgccacg tttctgttcc gccagttctt tatgacccta ccggatgaat
    55681 tggtggaagc cgcgcggatc gacggcgcgt caccaatgcg tttcttttgc gacatcgttt
    55741 ttccgttgtc caaaactaat ctggcggcgc tgtttgtgat cacctttatc tacggctgga
    55801 atcagtattt gtggccgttg ttgattatta ccgacgttga tctcggcacc accgtggcag
    55861 ggatcaaagg gatgatcgct acaggcgaag gcaccacgga atggaactcg gtaatggcgg
    55921 cgatgttgtt aacgcttatc cctccggtgg tgattgtttt agtgatgcag cgtgccttcg
    55981 tgcgcggcct ggtcgatagt gagaaataag atggcaggac tgaaattaca ggcagtaacc
    56041 aaaagctggg atggcaaaac ccaggtcatt aaaccgctga cccttgatgt ggcggatggc
    56101 gaatttatcg tgatggtcgg gccgtctggc tgcgggaaat cgacgctgct gcgcatggtt
    56161 gccgggctgg agcgggtgac agaaggcgat atctggatca acgaccagcg cgtgaccgaa
    56221 atggagccga aagatcgcgg gattgcgatg gtgttccaga attacgcgct ttatccgcat
    56281 atgagcgtag aggagaacat ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa
    56341 attgccgagc gcgttaaaga agcggcgcgc attctggaac tggacggtct gcttaagcgt
    56401 cgtccgcgtg agctatccgg cggtcagcgc cagcgtgtgg cgatgggccg cgcgattgtg
    56461 cgcgatccgg cggtgttctt gtttgatgag ccgctctcta acctcgatgc caagctgcgc
    56521 gtgcagatgc gtcttgaact gcaacagttg caccgtcgcc tgaaaacgac ttcactctac
    56581 gttactcacg atcaggttga agcgatgacg ctcgcccagc gagtaatggt gatgaacggt
    56641 ggcgttgccg aacagattgg cacaccagtt gaagtctacg aaaagcccgc cagcctgttt
    56701 gtggcgagtt ttatcggcag cccggcgatg aacctgctga caggccgcgt gaataatgaa
    56761 ggcacgcatt tcgaactgga cggcggtatc gtgctaccgc taaacggtgg ctaccgtcag
    56821 tatgccgggc gtaaaatgac tctcggcatt cgcccggaac atatagcgct aagctcgcag
    56881 gcagaaggtg gtgtgccgct ggtgatggac acgctggaga tcctcggcgc agataacctg
    56941 gcgcacggac gctggggcga gcagaagctg gtggtgcgac tggcgcatca ggagcgcccg
    57001 acggcaggca gcacgctgtg gctgcatttg gcggaaaatc agctgcatct ttttgatggt
    57061 gaaacaggac aacgagtatg agtaactggc cttatccccg catcgtcgct catcgtggcg
    57121 gcggtaagct ggccccggaa aacaccctgg cggcaatcga cgtcggggca aaatacggtc
    57181 ataagatgat cgaatttgac gcgaagttat cgaaagatgg cgagatcttc ctgctccatg
    57241 acgacaatct cgaacgcacc agcaacggct ggggcgtcgc gggtgaactg aactggcagg
    57301 atttactgcg cgtggatgcg ggcagttggt acagcaaagc gtttaaaggt gagccgctgc
    57361 cgttgctttc gcaggtggcg gaacgctgtc gcgaacacgg gatgatggcg aatatcgaaa
    57421 tcaaacccac cactggcact ggaccattaa cgggcaaaat ggtggcgctg gcggcacgcc
    57481 aactgtgggc cggtatgacg ccgccgctgc tgtcatcgtt tgagattgat gctttagaag
    57541 ctgcacaaca ggcggcaccg gaactgccgc gtggtttatt gcttgatgag tggcgcgacg
    57601 actggcgcga actgaccgcg cggctgggct gcgtctctat tcatctcaat cataagttac
    57661 tcgataaagc gcgagtgatg cagttgaaag acgccggatt acggattctg gtttataccg
    57721 tcaataaacc ccagcgcgcg gcagagttgc tgcgctgggg cgtggattgc atctgtaccg
    57781 atgcgattga cgtgattggt ccgaacttta cggcccaata gttttcaacg gaatgtcagg
    57841 ctgcggcgtg ctgggctgat tcagcatgtc gccgtttctc ctttccggca gcatatgctg
    57901 ctgattcaac gaactatccg gattacggtt gctgtttaac atcccgccgt tggtgttggg
    57961 cagcatttgc tgccgggcgg gatttcgctc ccccggctgt gactgcaaca cccgctgaga
    58021 attattgttt atctggtttt ctaaatgctg ctgttgcaac tgagtttgcg ttttcagttg
    58081 ctgattcagc atcccttttt gctggatttg ctgagtctgc atctgggttt gcatccgctg
    58141 ctggctgggt atctgatacc ccggctggtt agggttgttc agagtattaa tgggctgtgc
    58201 aaagccgaca aacggcagga gtgccgtaag aatcagaagt cgtttcatcg cgtatcctcc
    58261 tctgaagata tcctttaagt ttactcgctt cccgacaaaa cgatgattaa ttcagagtta
    58321 tataccaggc ttagctgggg ttgcccctta atctctggag aataacgatg ataaaaccga
    58381 cgtttttacg ccgggtggcc attgctgctc tgctctcagg aagttgtttt agcgccgccg
    58441 ccgcgcctcc tgcgccgccc gtctcgtatg gtgtggagga agatgtcttc cacccggtac
    58501 gcgcgaaaca gggaatggta gcgtctgtgg acgccactgc cactcaggtg ggggtggata
    58561 tcctcaagga gggcgggaat gccgttgatg ccgccgtggc ggtgggctac gcgctggcgg
    58621 taacgcatcc gcaggcaggg aatctgggcg gtggtggttt tatgttaatc cgctcgaaaa
    58681 atggcaatac cacggctatc gatttccgcg aaatggcacc cgccaaagcg acccgcgata
    58741 tgttcctcga tgatcagggc aacccggaca gcaaaaaatc actcacttcg catctggctt
    58801 ccggcacacc gggtacggta gcaggtttct cgctggcgct ggataaatac ggcaccatgc
    58861 cgctgaacaa agtcgtgcag cccgcgttta aactggcacg cgatggtttt atcgttaacg
    58921 acgcgctggc tgacgatctc aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca
    58981 gtaaagctat cttctggaaa gagggcgagc cgctgaaaaa gggcgacacg ctggtgcagg
    59041 cgaacctggc aaagagcctg gagatgattg ctgaaaacgg cccggacgaa ttctataaag
    59101 gcacgattgc ggaacagatc gcccaggaga tgcagaaaaa cggtggcttg atcactaaag
    59161 aagatttagc ggcctataaa gcggtcgaac gcactccgat aagcggcgat tatcgcgggt
    59221 atcaggttta ctccatgcca ccgccatcct ccggcgggat ccatatcgta caaatcctca
    59281 atattctgga aaacttcgat atgaagaaat acggctttgg cagcgccgat gcgatgcaaa
    59341 tcatggcaga agcggagaaa tacgcctacg ccgaccgctc ggaatatctt ggcgacccgg
    59401 attttgtcaa agtcccgtgg caggcactga ccaataaagc ctatgccaaa tccattgccg
    59461 atcaaattga tatcaacaaa gcgaagccgt ccagtgagat tcgccctggc aagcttgcgc
    59521 cttatgagag taatcaaact acccattact ctgtagtgga taaagacggt aacgcggtgg
    59581 cggtgaccta tacgctgaac accaccttcg gtacgggcat tgtcgcgggc gagagcggta
    59641 ttctgcttaa taatcagatg gatgatttct ccgccaaacc gggcgtaccg aacatttacg
    59701 ggctggtggg cggtgatgcc aacgccgtcg ggccgaataa acgtccgctg tcatcgatgt
    59761 caccaaccat tgtggtgaaa gacggtaaaa cttggctggt taccggtagt cctggcggga
    59821 gtcggattat taccaccgtg c
//
LOCUS       DAJTYZ010000007        59059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000007 DAJTYZ010000000
VERSION     DAJTYZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.007"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(180..1127)
                     /gene="dusC"
                     /locus_tag="OO596_000417"
     CDS             complement(180..1127)
                     /gene="dusC"
                     /locus_tag="OO596_000417"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6716376.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1366..1764
                     /locus_tag="OO596_000418"
     CDS             1366..1764
                     /locus_tag="OO596_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6716377.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1761..2456
                     /locus_tag="OO596_000419"
     CDS             1761..2456
                     /locus_tag="OO596_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6716378.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2586..3470
                     /gene="cdd"
                     /locus_tag="OO596_000420"
     CDS             2586..3470
                     /gene="cdd"
                     /locus_tag="OO596_000420"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6716379.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3620..4339
                     /gene="sanA"
                     /locus_tag="OO596_000421"
     CDS             3620..4339
                     /gene="sanA"
                     /locus_tag="OO596_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6716380.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4342..4581
                     /locus_tag="OO596_000422"
     CDS             4342..4581
                     /locus_tag="OO596_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6716381.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4775..6013
                     /gene="preT"
                     /locus_tag="OO596_000423"
                     /pseudo
     CDS             4775..6013
                     /gene="preT"
                     /locus_tag="OO596_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            6007..7242
                     /gene="preA"
                     /locus_tag="OO596_000424"
     CDS             6007..7242
                     /gene="preA"
                     /locus_tag="OO596_000424"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR6716382.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7369..8379)
                     /gene="mglC"
                     /locus_tag="OO596_000425"
     CDS             complement(7369..8379)
                     /gene="mglC"
                     /locus_tag="OO596_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6716383.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8395..9915)
                     /gene="mglA"
                     /locus_tag="OO596_000426"
     CDS             complement(8395..9915)
                     /gene="mglA"
                     /locus_tag="OO596_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6716384.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(9976..10974)
                     /gene="mglB"
                     /locus_tag="OO596_000427"
     CDS             complement(9976..10974)
                     /gene="mglB"
                     /locus_tag="OO596_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6716385.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11254..12294)
                     /gene="galS"
                     /locus_tag="OO596_000428"
                     /pseudo
     CDS             complement(11254..12294)
                     /gene="galS"
                     /locus_tag="OO596_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(12436..13593)
                     /locus_tag="OO596_000429"
     CDS             complement(12436..13593)
                     /locus_tag="OO596_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6716386.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13610..14278)
                     /gene="folE"
                     /locus_tag="OO596_000430"
     CDS             complement(13610..14278)
                     /gene="folE"
                     /locus_tag="OO596_000430"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6716387.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVIIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14536..15372
                     /gene="yeiG"
                     /locus_tag="OO596_000431"
     CDS             14536..15372
                     /gene="yeiG"
                     /locus_tag="OO596_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6716388.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15404..17395)
                     /gene="cirA"
                     /locus_tag="OO596_000432"
     CDS             complement(15404..17395)
                     /gene="cirA"
                     /locus_tag="OO596_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR6716389.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17687..19156)
                     /gene="lysP"
                     /locus_tag="OO596_000433"
     CDS             complement(17687..19156)
                     /gene="lysP"
                     /locus_tag="OO596_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6716390.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19361..20242)
                     /locus_tag="OO596_000434"
     CDS             complement(19361..20242)
                     /locus_tag="OO596_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6716391.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20341..21390
                     /locus_tag="OO596_000435"
     CDS             20341..21390
                     /locus_tag="OO596_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6716392.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21464..22321
                     /gene="nfo"
                     /locus_tag="OO596_000436"
     CDS             21464..22321
                     /gene="nfo"
                     /locus_tag="OO596_000436"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6716393.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22325..23413
                     /locus_tag="OO596_000437"
     CDS             22325..23413
                     /locus_tag="OO596_000437"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6716394.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23469..24719)
                     /locus_tag="OO596_000438"
     CDS             complement(23469..24719)
                     /locus_tag="OO596_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6716395.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24819..25760)
                     /gene="rihB"
                     /locus_tag="OO596_000439"
     CDS             complement(24819..25760)
                     /gene="rihB"
                     /locus_tag="OO596_000439"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6716396.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25890..26588
                     /gene="yeiL"
                     /locus_tag="OO596_000440"
     CDS             25890..26588
                     /gene="yeiL"
                     /locus_tag="OO596_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCR6716397.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26655..27905)
                     /locus_tag="OO596_000441"
     CDS             complement(26655..27905)
                     /locus_tag="OO596_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6716398.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27999..28937)
                     /gene="psuG"
                     /locus_tag="OO596_000442"
     CDS             complement(27999..28937)
                     /gene="psuG"
                     /locus_tag="OO596_000442"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6716399.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28925..29866)
                     /locus_tag="OO596_000443"
     CDS             complement(28925..29866)
                     /locus_tag="OO596_000443"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6716400.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(30324..32015)
                     /gene="fruA"
                     /locus_tag="OO596_000444"
     CDS             complement(30324..32015)
                     /gene="fruA"
                     /locus_tag="OO596_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6716401.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(32032..32970)
                     /gene="fruK"
                     /locus_tag="OO596_000445"
     CDS             complement(32032..32970)
                     /gene="fruK"
                     /locus_tag="OO596_000445"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6716402.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32970..34100)
                     /gene="fruB"
                     /locus_tag="OO596_000446"
     CDS             complement(32970..34100)
                     /gene="fruB"
                     /locus_tag="OO596_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6716403.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34468..35649
                     /gene="setB"
                     /locus_tag="OO596_000447"
     CDS             34468..35649
                     /gene="setB"
                     /locus_tag="OO596_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6716404.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35646..35900)
                     /locus_tag="OO596_000448"
     CDS             complement(35646..35900)
                     /locus_tag="OO596_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6716405.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            36055..36627
                     /gene="yeiP"
                     /locus_tag="OO596_000449"
     CDS             36055..36627
                     /gene="yeiP"
                     /locus_tag="OO596_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6716406.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36850..38316
                     /locus_tag="OO596_000450"
     CDS             36850..38316
                     /locus_tag="OO596_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6716407.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38434..39420
                     /gene="yeiR"
                     /locus_tag="OO596_000451"
     CDS             38434..39420
                     /gene="yeiR"
                     /locus_tag="OO596_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6716408.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39459..40172
                     /gene="lpxT"
                     /locus_tag="OO596_000452"
     CDS             39459..40172
                     /gene="lpxT"
                     /locus_tag="OO596_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6716409.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40584..41150
                     /gene="mepS"
                     /locus_tag="OO596_000453"
     CDS             40584..41150
                     /gene="mepS"
                     /locus_tag="OO596_000453"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6716410.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41331..42887
                     /locus_tag="OO596_000454"
     CDS             41331..42887
                     /locus_tag="OO596_000454"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6716411.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            42969..44783
                     /locus_tag="OO596_000455"
     CDS             42969..44783
                     /locus_tag="OO596_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6716412.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44784..45878
                     /locus_tag="OO596_000456"
     CDS             44784..45878
                     /locus_tag="OO596_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6716413.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45878..46903
                     /locus_tag="OO596_000457"
     CDS             45878..46903
                     /locus_tag="OO596_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6716414.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46905..48494
                     /gene="yejF"
                     /locus_tag="OO596_000458"
     CDS             46905..48494
                     /gene="yejF"
                     /locus_tag="OO596_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6716415.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(48498..48842)
                     /locus_tag="OO596_000459"
     CDS             complement(48498..48842)
                     /locus_tag="OO596_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716416.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49175..50295)
                     /locus_tag="OO596_000460"
                     /pseudo
     CDS             complement(49175..50295)
                     /locus_tag="OO596_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(50323..51018)
                     /gene="rsuA"
                     /locus_tag="OO596_000461"
     CDS             complement(50323..51018)
                     /gene="rsuA"
                     /locus_tag="OO596_000461"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6716417.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51168..52928
                     /locus_tag="OO596_000462"
     CDS             51168..52928
                     /locus_tag="OO596_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6716418.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            53053..53337
                     /gene="rplY"
                     /locus_tag="OO596_000463"
     CDS             53053..53337
                     /gene="rplY"
                     /locus_tag="OO596_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6716419.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53476..54483)
                     /gene="yejK"
                     /locus_tag="OO596_000464"
     CDS             complement(53476..54483)
                     /gene="yejK"
                     /locus_tag="OO596_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6716420.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54665..54892
                     /locus_tag="OO596_000465"
     CDS             54665..54892
                     /locus_tag="OO596_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6716421.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54912..56672
                     /gene="pbgA"
                     /locus_tag="OO596_000466"
     CDS             54912..56672
                     /gene="pbgA"
                     /locus_tag="OO596_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6716422.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56747..56823
                     /locus_tag="OO596_000467"
     tRNA            56747..56823
                     /locus_tag="OO596_000467"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56781..56783,aa:Pro,seq:ggg)
     gene            complement(56926..58928)
                     /locus_tag="OO596_000468"
                     /pseudo
     CDS             complement(56926..58928)
                     /locus_tag="OO596_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT        14537 a        15415 c        14413 g        14694 t
ORIGIN      
        1 cccttacggc taccgggtcg ggcgcgaggt aacatccctg taaaacgcgc cctcagccca
       61 cctccatgtg ggctgccccg gccttcaggg aacgcctcgg caattttgac gccaccaaac
      121 aaccgtgcgg cctattgata aagagctaac acattgtcaa aaaacatcac tatggttttt
      181 tagagtttct cgatatcaat tgcctgaata gcccttgcaa tatcagggga attattcaac
      241 acccgaacat gctgaaataa ttccgttgct tcatcgtatt ctttacgcaa ataactcaac
      301 cactgtttaa tccgcgcaac gtgatataac ccggtatcgc cctgcttttc cagacgggta
      361 tatttttgca gcaaagcaac cacctccggc cacggcattc gcggttcgtt atattttact
      421 acccggctca ggttgggaat attgagcgcc ccgcgaccaa tcatcactgc gtcgcagccg
      481 ctgatcgcaa tgcattgttg cgcgctctgc cagtcccaga tttcgccgtt ggcaatcacc
      541 ggaatattca gccgctggcg aatctcgcca atcgcctgcc agtcaatatg ctccgcgcgg
      601 taaccctgct cttttgtccg tccatgcacc accagctccg tagcaccagc ctgttgaacc
      661 gcatcggcga tttcaaattt cttctcaccg ctgtcccagc ccagacgcac tttcacgctg
      721 acgggcaaat gcgccggtac agcttcacgc atcgcttttg caccctggta gatgagttca
      781 ggatctttga gtaacgtcgc cccgccgccg ctgccgttaa ccgttttcga cgggcagccg
      841 caattgagat ccacgcccca ggaacctaat tccaccgcac gggcggcgtt ctctgccagc
      901 cattgtggga actgacctaa caactgaacg cgcaccagcg taccggatgg tgtccggctg
      961 gcgttttgta gctcagggca aatgcgatga aagactttta ccggcagcag ttgatccacc
     1021 acgcggacaa actcggtgat gcacagatcg tagtcgttaa cttcggtcag caattctcgc
     1081 accagagagt caagcacacc ctccatcgga gccagtaaca cacgcatatc ttcacccgga
     1141 aaaaaatgag gcgctatgtt agcgcctatg gtcaacggat taaaggtctg caaagaggga
     1201 ttgctgcaaa ctgtaatccc tctctatgct tgatgtatgc ctgagttcgt tgcgcattgc
     1261 gtaatgccgg ggagcgaaaa aataagcgct gatttcttaa tgtgatcggt agcacgtttt
     1321 acgaattaat tgtatgatga atccatctca tctggggtgt tgattatgag taagacactg
     1381 aacattatct ggcaatattt acgcgctttc gtcctgattt atgcctgcct gtatgcaggc
     1441 attttcattg cttccctgct accggtaacc attccgggca gcatcatcgg gatgctgatc
     1501 ctgtttgtcc tgctggcctt gcaaattctt ccggcaaaat gggtcaatcc ggggtgctac
     1561 gtactgattc gctatatggc gctattgttt gtgccgattg gcgtaggcgt catgcaatat
     1621 tttgatttgc tccgcgcaca gtttggcccg gtagtggttt cctgtgcagt cagtacgctg
     1681 gtggtttttc tggtggtgag ctggagttca caactggtac acggtgaacg taaagtcgta
     1741 ggtcagaaag gatcagaaga atgatggcga atatctggtg gtcattaccg ctgacgttga
     1801 ttgtcttttt tgccgcccgc aaactggcgg cacggtataa gtttcccctg cttaacccgt
     1861 tgctggtagc aatggtggtg atcattcctt ttttaatgct gaccggcatc tcttacgaca
     1921 gctactttaa aggtagcgaa gtgctaaacg atctgctgca accggcggtc gtcgcgctgg
     1981 cctatccttt atatgagcag ctacaccaga tccgcgcgcg ctggaaatcg atcatcacca
     2041 tctgttttat cggcagcgtg gttgcgatgg tgacggggac ttccgtggca ttgttgatgg
     2101 gcgcttcacc ggaaatcgcc gcgtcaatcc tgccgaaatc agtcaccacg cctattgcaa
     2161 tggcggttgg cggcagtatt ggcggtattc cggcaatcag cgcagtttgc gtgattttcg
     2221 tcggcatcct cggcgcggta tttggccata cattgcttaa tgcgatgcgt attcgtacca
     2281 aagctgcgcg cggtctggca atggggacag cctcgcacgc cctcggtacg gcgcgctgcg
     2341 ccgagctgga ttatcaggaa ggtgcattta gttcgctggc gctggtgtta tgcgggataa
     2401 ttacttcgct gatcgcaccg ttccttttcc cgattattct ggcagtaatg ggctaaaatt
     2461 tgcgatgcgt cgcgcatttt tgatgtaagt ttcacgcgtt gcataattaa tgagattcag
     2521 atcacatata aagccataac gggttcgtaa actgttatcc cattacatga ttatgaggca
     2581 acgccatgca tccacgtttt caaaccgctt ttgcccaact tgcggataac ttgcaatctg
     2641 cactggaacc tattctggca gacaagtact tccccgcttt gttgaccggg gagcaagtct
     2701 catcgctgaa gagcgcaacg gggctggacg aagacgcgct ggcattcgca ctacttccac
     2761 tggcggcggc ctgtgcgcgt acgccattgt cgaattttaa tgttggcgca attgcgcgcg
     2821 gtgtgagcgg aacctggtat ttcggtgcca atatggaatt tattggtgca acaatgcagc
     2881 aaaccgttca tgccgaacaa agcgcgatca gccacgcctg gttgagtggt gaaaaagcgc
     2941 ttgcagccat caccgttaac tatacgcctt gtggtcactg ccgtcagttt atgaatgaac
     3001 tgaacagcgg tctggatctg cgtattcatc tgccgggccg cgaggcacac gcgctgcgtg
     3061 actatctgcc ggatgccttt gggccgaaag atctggagat taaaacgctg ctgatggacg
     3121 aacaggatca cggctatgcg ctgacgggtg atgcgctttc tcaggcagcg attgcggcgg
     3181 caaaccgttc gcacatgcct tacagtaagt cgccaagcgg tgtcgcgctg gaatgtaaag
     3241 acggtcgtat tttcagcggc agctacgctg aaaacgccgc attcaacccg actctgccac
     3301 cgttgcaggg ggcgttaatt ctgttgaatc tcaagggtta tgattacccg gatatccagc
     3361 gcgcggttct ggcagaaaaa gccgatgcgc cgttgattca gtgggatgcc acctccgcaa
     3421 cgctgaaagc tctcggctgt cacagtatcg accgcgtgct tctcgcttaa gcctggtgcc
     3481 ggatgcggcg tgaacgcctt gtccggcttg ccagccctct cctggtgtcg aaattcccgg
     3541 caaacagttt gccgtttctt gcgcaaaacc agcgggtaaa gtagcctgat ggaaattttc
     3601 cttagatcga gtctcctgca tgttaaagcg cgtgttcctc agcctgttag tcctgatcgg
     3661 cttgctgctg ttgactgtgc tcggcctcga tcgctggatg agctggaaaa ccgcgcctta
     3721 tatctacgac gaattgcagg atctccccta ccgccaggtc ggcgtggtgc tcggcacagc
     3781 aaaatattat cgtactggcg taattaatca gtattatcgc taccgcattc aaggagcgat
     3841 taatgcctat aacagcggta aggtaaatta tctattactg agcggcgata acgcattgca
     3901 aagttataat gagccgatga ccatgcgcaa agatttaatc gctgctggtg tcgacccatc
     3961 agatattgtt ctcgattacg caggctttcg tacgctggat tccatcgtgc gtacacgcaa
     4021 agtgttcgat actaatgatt tcattattat cacccaacgt ttccactgtg agcgggcatt
     4081 atttattgcg ctgcatatgg ggattcaggc tcagtgttat gccgtaccgt caccgaaaga
     4141 tatgctgtca gtacgtattc gcgaatttgc cgcccgtttc ggtgcgctgg ctgaccttta
     4201 tatttttaga cgtgaaccgc gttttttagg gccgctggtc cctattccgg ctatgcacca
     4261 ggtgccggaa gatgcgcagg ggtatcccgc cgtcacaccc gaacagttac ttgaattaca
     4321 aaagaaacaa ggaaagtagt tatggatgta cagcagtttt ttgtcgttgc cgtttttttc
     4381 cttatcccga tattttgttt ccgcgaagca tggaaaggct ggcgcgcagg cgcgattgat
     4441 aaacgggtta aaaatgcacc ggaaccggtg tatgtctggc gagcaaaaaa tcccggactc
     4501 tttttcgctt atatggtggc atatatcggc ttcggaattt tatctatcgg catgattgtt
     4561 tatcttattt tctatcgtta atattttctc ttttctgtta attattgaga ataattatta
     4621 cttcactcaa taagctgcgg atataacatt ctgaaccgca gctatttgtg aatcctttca
     4681 cagtttaaat ttccccacac gcttagcctt aatatcagca cgcaatattt atttagcgtt
     4741 cgccttaatt actcatagca ttaaggaaga tcacatgccg caacaaaatt atctggatga
     4801 actcactccg gcttttacgc ctttactggc gattaaagaa gcctctcgct gtttattatg
     4861 tcatgacgct ccctgtagtc aggcttgccc ggcacagacc gatccgggga aatttattcg
     4921 ctcaatctac tttcgtaatt ttaaaggcgc tgccgagaca attcgcgaaa ataatgccct
     4981 cggtgccgtt tgtgccagag tgtgcccgac ggagaaatta tgccaaagcg gttgtacccg
     5041 tgccggtgtt gatgctccca ttgatatcgg ccgcttacag cgttttgtta ctgattttga
     5101 acaacaaacc gggatggaaa tttatcagcc cggtactaaa acgctcggca aagtcgcgat
     5161 tattggcgca ggtcctgccg gattgcaggc cagtgtgaca ctgacaaacc agggttatga
     5221 cgtcacgatc tatgagaaag aagcacaacc cggtggctga ttgcgtaacg gcattccgca
     5281 attccggtta ccgcaatcag tactggatgc agagatcgcc cgtattgaaa aaatgggcgt
     5341 gaccattaag tgcaacaacg aagtcggtaa cacactcacc cttgagcagc tgaaagcaga
     5401 aaaccgcgcg gtactggtca ccgtggggtt atcaagcggt tccgggctac cgctgtttga
     5461 gcatagtgac gttgagattg ccgtcgactt cttgcaacgt gcacgacagg cgcaaggcga
     5521 tatcagcatt ccacaaagcg cattaattat cggcggcggt gatgtcgcga tggacgtagc
     5581 cagcacgctg aaagttctcg gctgtcaggc ggtaacttgc gtagcgcgtg aagagttaga
     5641 tgagtttccg gcaagcgaaa aagagtttac cagcgcccgg gagctgggcg tttcgatcat
     5701 cgatggattc acgccagtag ccgtggaagg caataaagtc acgtttaagc atgtacggtt
     5761 atcgggcgaa ctgacgatgg cggcagataa aattattctc gccgtcggtc agcatgccag
     5821 actagatgcc tttgcggagt tagagccgca gcgtaacacc atcaaaacac aaaattacca
     5881 gacccgcgac ccgcaagtct ttgctgctgg cgatattgtt gagggagaca aaaccgtggt
     5941 ctatgccgtg aaaaccggga aagaagccgc cgaggcgatt catcactatt tagagggagc
     6001 ttgctcatgt taacgaaaga tctttcgatt actttttgcg gcgtgaagtt tcccaacccg
     6061 ttctgcctct cttcttcgcc ggtaggcaac tgctatgaga tgtgtgccaa agcctacgac
     6121 acaggttggg gcggtgtggt gtttaaaacg atcggctttt ttatcgccaa cgaagtctcg
     6181 ccgcgttttg atcatctggt gaaagaagat accggtttta tcggcttcaa aaatatggag
     6241 cagattgccg agcatccgct ggaggagaat ctggccgccc tgcgtcggct gaaagaagat
     6301 tacccggaca aagtattgat cgcttcgatc atgggggaaa atgagcagca atgggaggag
     6361 ctggcgcgcc tggtgcaaga agctggcgcg gatatgatcg agtgtaactt ctcctgtccg
     6421 caaatgactt ctcatgcgat gggtagcgat gtcgggcaaa gcccggagct ggtagaaaaa
     6481 tattgtcggg cagtgaaacg gggttccacg ctgccaatgc tggcgaagat gacgccgaat
     6541 atcggtgata tgtgcgaagt ggcgctggcg gcgaagcgcg gcggcgcaga tggcattgcg
     6601 gcgattaaca ccgttaaatc catcaccaat atcgatctta atcagaaaat cggtatgccg
     6661 atcgttaacg gaaaatcaag tatttccgga tattccggta aagcggtaaa accgatcgcc
     6721 ctgcgcttca ttcagcaaat gcgcacccat ccagaactgc gcgatttccc aatcagcggt
     6781 atcggcggca ttgaaacctg ggaggatgcg gcggagtttt tattgctcgg cgcagcaacg
     6841 ttacaggtca ccaccggcat tatgcagtac ggctatcgca tcgtagaaga tatggcgagc
     6901 gggttatcgc attatctcgc cgatcaggga tttgattcgt tgcaggagat ggtaggtctg
     6961 gcgaataaca atattgtccc ggcggaagat ttagaccgca gttatattgt ctatccccgt
     7021 atcaatcttg acaaatgtgt tggctgtgga cgctgttata tttcctgtta cgacggcggt
     7081 caccaggcga tggaatggag cgagaaaacc cgcacaccgc attgtaatac cgagaaatgt
     7141 gtgggttgtc tgctttgtgg tcacgtctgc ccggtgggtt gtattgagct cggggaagtg
     7201 aagtttaaga aaggcgagaa agaacacccg gtaacgttgt agaacccgcg tcacatccgg
     7261 catttggttc ctgttgccgg atgcggcgtg aacgccttat ccggcctaca aaaccacaca
     7321 gatcatgatg ttatgtgcgt ttatgaccga atgcggacca cattcacatc atttcttacg
     7381 cgcgtatttc agtgaatcca gcgctacggc gaagataata atcgcccctt tgatgatgta
     7441 ctgccagtat gggtttacgc cgatatacgt caggccatag ttgatgacgg taaaaataat
     7501 caccccggtc accacgccaa tcaccgtccc cacaccgccg ctgaacgata caccgcctac
     7561 cacgcacgcc gcgatagcat ccagctcata cataaagccg aggttgttgg tggcagagcc
     7621 gatacgtccg gcttctaaca tcccgccaaa ggcatagaac acgccagaca acgcgtagat
     7681 catcagcagg ttcaggccga cgttgacacc agatactttt gccgcttccg ggttaccgcc
     7741 aatggcaaaa atgttcttac cgaagcgggt tttgttccac aacacccaga cgaacgccac
     7801 cgcaatcaac gcgtagaagg tgatgtaaga gagacggaaa ctccccagcg cgacaaagcc
     7861 ctgagcaaag gtagagaagc cactgtcaaa accagaaatt ggcgacgccc cgacaaagtc
     7921 gtaatagagc gagttgatgc catagacgat gatcatcgtg ccgagcgtgg taatgaacgg
     7981 cgtcacgttg agataagcga taatcagacc gttgatcaaa ccgatcaccg caccaatggc
     8041 acagacaatc agaataacca gcgcaatcgg catcgtcgcc atttccggga acactttgtt
     8101 ggcgttatcc atggactgca ataatgtcgc agccaccact gccgccagcc ctacctgacg
     8161 accagcagaa agatcggtcc cctgggtgac aattaaccct gccacaccga gcgcgataat
     8221 aatacgcacc gatgactggg tgagaatatt acttaagttc aacagactta aaaatgttgg
     8281 gtcctggaaa ataataatcg ccagcaaaac taaaagaacg acgtaaatac cgccctcttt
     8341 caggtaagta agaaaacttt tcttatttaa cgcactcatg ggaagcccct aatcttaaag
     8401 gtgcaaagac gcaagacgca gaatttcgtt ttgcgttgtt gtttttgtat cgacaattcc
     8461 ggaaacgaga ccattgctca tgaccagaat acggtctgtt atccctaaca actcaggcat
     8521 ttcagaggag ataataataa tccccttgcc tttcttcgcc agttcggcaa ttaactgata
     8581 aatttcaaac ttcgccccga catcaatacc gcgcgtcggt tcatcgagca ttaatatttc
     8641 tggttgcgtt agtagccagc gaccaataat caccttttgc tgattaccac cggagagcga
     8701 accaatttgc gtccgatgac ccggcgtttt tacccgcatc gaatcaatca cccactgggt
     8761 atcgcttttc atccgcgagt tatccagtaa accaacttta tttttgtagt tgcgaatatt
     8821 ggaaattaac gagttaaaac caatatccag ataggcataa attcccgttg agcggcgctc
     8881 ctcagttacc agtgcaaatc cgtggtttat ggcttcgttg gcattatggt tattgatctg
     8941 tttgccgtgc aacgtaatgg tgccagccga tttctcgcga ataccaaata acgtctcaac
     9001 aatatcggta cgtttcgccc ccaccagccc ggcaataccg aggatctccc ctttatgcag
     9061 atcaaacgag acatcgcgaa tcgacggctg gcgcagtgac gttaggttac gcacctcgag
     9121 gatgacttcg cccggcttgt tttctttgtc aggaaaacgc tgattaagag aacgcccaac
     9181 catcatggcg atgatcttgt ccatcgtcag tcctgccagc ggttcggtgg cgatccactg
     9241 accgtcgcgc aatacggtaa cttcatcaca taactggaag atttcttcca ttttatgcga
     9301 gatataaaca ataccgcagc cgcgctcttt taatttacga ataatagtga acagatgatt
     9361 gacctctttt tcggttaacg aagaagtcgg ttcatccata atcacaattt tcgcgttata
     9421 ggaaaacgct ttggcgattt cgatcatctg catttgcgaa acggataatg tgccgacgcg
     9481 cgcacgcgga tcgatatcaa tatccagttc atcaaaaatt gctttggttt cgcggtacat
     9541 tttgtcctga tcgacaaaca tgcctttggt gggatatcgc cccagccaca tgttatccat
     9601 caccgaacgt tgtaatacca ggtttaactc ctggtgtacc atcgaaatac cattttccag
     9661 tgcctctttg gcggaatgga aatcgatctc tttaccctgg aataaaatgg tgccggagtc
     9721 tttttgataa ataccaaaca ggcattttaa taatgtcgat tttcctgcac cgttttcccc
     9781 cattaatgca tggatagaat gtggccggac ttttaaatta acgttatcaa gtgccttaac
     9841 accaggaaag gacttgttga taccgctcat ttccaacaag tattccccgg aagacggagt
     9901 cgttgagctg accatataat tttaccttgt tggccataca ataagggcgc agtaaacgac
     9961 tgcgcccaat cagtcttatt tcttgctgaa ttcagccagg ttgtctttat ctacgccaac
    10021 ataaggtacg cggaccactt tgttgtcgat tttccagttg gtgccatcag ccgcaccttt
    10081 gccatcggcc aggtttttcg ccagatcaaa ggtcgctttc gcctggttgt tagcatcgtt
    10141 cagtactgtg cccgccagtg caccggattt caccagcgcc agcgcttctg gcagcgcatc
    10201 gacgccaaac accggaatgc tggacttgtt gtgtgctttc agtgcttcta ccgcgcccat
    10261 tgccatcgca tcgttgttgg cgataaccac ttcgattttg ttggcgttcg ggccagacag
    10321 ccaggcgtcc atcttatctt tcgcctgagc ggtgtcccac attgcggtat ctaactgtaa
    10381 ctgttcagtt ttgatgcctt tatcgttcaa ttctttaatc acgtaagtgg tacgtgcttc
    10441 tgcatccgga tggcccggtt cacctttcag cagtacgaac tgaatctgac cgtctttgtt
    10501 cagatcccaa ccctgattcg ccgcccagtg tttagcaatc aaatcgccct gaataatgcc
    10561 ggactctttg gagtcagtgc caacgtagta ggctttgtcg tagctatcca gcgccttacg
    10621 agacggttct ttgttgaaga aaaccaccgg cacgttttgc ccacgcgctt tctcaatcac
    10681 cgtacccgca gctgccgggt caaccaggtt gattgccagt gccttcaccc ctttcgccag
    10741 caatacgtcg atctgatcgt tctgcttgga ctggtcattc tgagaatcat tcatcagcag
    10801 ctgaacatct ggcgcggctt tcgcatcttg ctcaatagcc ttgcgcacta cagacataaa
    10861 gttatcgtcg tacttataga ttgttacacc aatgcgagta tcagcagcgt gtgcagcggc
    10921 accgaataac atgctggcca tcacagcaga cagggttaac accttcttat tcatggtatc
    10981 tccggttttt cttatgcagg gtagtgcttg agataaatgc tcggcggggc agtagagtta
    11041 atgaagtgtt actgaacgcc gaagctcact tttttaaatt cgttcttcca tgcccggtaa
    11101 cgctccagaa aacggcttta attgttgtta tgacgctatt acctcggtga aagtgattat
    11161 tcactgttac atacgggtta caacgttaaa acggtgcaat catagctatc acattgttaa
    11221 gatactgtga aatcactcac agattgaaag cggttacatc gcctgatttg ttgagttagt
    11281 gatcgccgcc gcattctggc gcgttgcgac agaatggcga cgcactaacg tcggcataaa
    11341 acagtggctg gcacgaggat caatattgcc tgctgccccc tgcaaggcca gttcggtggc
    11401 taatttagcc attgaagcaa tgggataacg cacggtcgtt aattgcgggt cggtgtaacg
    11461 ggcgatggga atatcatcga aaccgatgat tgagagatgt aacggaatcg caatgccatt
    11521 atcttttaat gctgtcagtg cgccagcggc catattgtcg ttataagcaa atatagcggt
    11581 aagttgtaga ttgcgcccca gcagttcaac catcgccgcc tcaccgcccg gcatgtccgg
    11641 cgtaccagtg ccaatccagc tttccggcgg aataatatcc tgctctttca acgcactcat
    11701 ccagcctgct ttacgcatgg cgtcatcttc aatgccgtga ctggaagaaa gataaccaat
    11761 acgttgatga ccgttattca gcaacatgcg cgtcgccatt cgggcaccgc tgagattatc
    11821 caggcaaacg caacgatggg cgtaccccgg cacaacgcgg ttgattaaca ccataccggg
    11881 aatgttatcc ataaattgcg ccagttcatc gtcactcaat gcttttgagt gaacaatcaa
    11941 cgcattacaa cgctggcgaa ttaacacctc aatggcgtga cgctcttttt ccgcttcatg
    12001 atagctattg ccgattagca cgtatttctg atgctgctga gcgactagat ccaccgcttt
    12061 taccagcgcg ccgaaaaacg catcagaaac gtccatcacc accacgccaa tggtgtcgct
    12121 aacctgagtt gccagcgcct gggcattggc gtttggccga taatccagct cactcacggc
    12181 tttcattact gcttcacgcg tgtcggcact gaccagcgtg ctgttattga gcacccggga
    12241 aaccgttgcc acagagacgc cagcctaacg cgctacatca cgaatggtga tcatattcac
    12301 tacctgttat gagattgcag taaatgactg cttgctggcg gctattttgt cagcactgaa
    12361 tacaggactt cgtgaatcga gtcacagcaa tggaaacggt tacagccgtt ttgttaacga
    12421 atgtgatcca gatcgttatc ttgatgtttt agatattgcc gttccggcag cacgcaaagt
    12481 taactgacgc cagagccatt ccaccggccc ctggcggaag taacgcagcc agataacaga
    12541 gaagaggata ttcgccagcc ataccggaat aacaaacgcc agcagctcca ggcggtcaaa
    12601 atgcataaac aaaccgaggt ggtaaaaaag catggtacaa atcagcgttt gcaatagata
    12661 gttggtcagc gccatccgtc cgacgcaggc gatcgcaagc accagcttaa agcggctcaa
    12721 ttgcggccag aagccataaa acagcgacgc atagccaatc gcctgaaacg gcgcactcag
    12781 ttcccgcggc atttgaagta agaaggcaca ccagcgatac gcccagtcca gctgccattg
    12841 cagggcgatg gcaggaaggt taatggtcac cccaatcgcc accagcacaa aaccagtacg
    12901 acgatagtga cgtaagctga actgcccttt cagccagccg ctgcgcatca gcgccgcacc
    12961 aatgagcatc atccctgcca gttgccagcc atactgtgcg cccagtgcca gtaaactgtt
    13021 gccaacacca tcggcacgat tactgatcgc ttcaacgccg ccgtgaagct tccagtattt
    13081 ttcatacaaa atagccgatg catccggcgt ccaggtgcgg ctggtctggc tgtcggaaat
    13141 cagccccaat aacagcaaaa cgccaagccc caccagataa agcatgacgc ctgtattaaa
    13201 caggcttttt accgatggcg catcgcgcac cagccgccag cagattaagc ccaccagccc
    13261 gtaagccagc agaatatcgc cgtcccagaa caataaaccg tgaataaaac ccagcaagac
    13321 taacagcgtt aaccgcgact ggatccagcg tctgccacgg ggcagcaaca tttgcaggcc
    13381 cgcaccaaac agcagcgcaa aaagcgtgag gaatttcacc tggccgatga gatcgagaaa
    13441 tgcccaggtc catgcatcct gcggcgtaat agcgccgtac caggcgggat tgagataagc
    13501 cgcctttggt agcccaaagg cgctgatgtt taatagcagg atccccagaa tggcgacgcc
    13561 gcgaacaaaa tcgagcgtta cgttgcgctc catggttcct gccttttaat cagttgtgat
    13621 gacgcacagc gcgcagaaac tcgtggcgcg tattctgact ggatttgaac aatccaccaa
    13681 gagaggtcgt tgttgtggca ctggttgcat cgcggatgcc acgcgctttc acgcagtaat
    13741 gcaccgcatc aatggagaca gccacgttat tggtgcccag cagcgtttgt agcgcaataa
    13801 gaatttgctg cgtcagacgt tcctgcacct gcggacgctg ggcaaagaac tgcacgatac
    13861 ggttaatttt tgacagaccg atcaccgaat ctttcgggat ataagccacc gtcgctttgc
    13921 catcgatgat aacaaaatgg tgttcacagg tactggtcag agtgatatcg cgcacggtga
    13981 ccatttcatc gaccttcatt ttgttttcaa tgagggtgat tttcgggaag ttggcgtaat
    14041 ccagaccgga gaaaatttca tcgacataca ttttagcgat gcgatgcggc gtttccatca
    14101 aactgtcatc agccaggtcg agattcagca gctgcatgat ttcggtcata tgaccagcaa
    14161 taaggctttt gcgcgtttcg ttatccattt catgcacggg cgggcgcagc ggtgtttcca
    14221 gtcctcgcgc aactaacgct tcatgaacca gggccgcttc tttactgagt gatggcattt
    14281 atgatttctc ctgcaggtgt gacgcctccg ccctgcgtgg gggcaaagtt attaagctga
    14341 tttacagcct gattattgtg cgtgaggcgg cgcacataat ccagtattca cagcgataat
    14401 tattgtaatt gccgctgact ttcatcagca gatgttaaaa catcgttatg caaatacgga
    14461 agtgaaagtt actcacagca cattgaataa acggtatgat gaagaaattg caaacaacac
    14521 aacaaggagc cacgcatgga aatgctcgaa gagcaccgct gttttgaagg ctggcagcaa
    14581 cgctggcgac acgactccag taccttaaac tgcccgatga cgttcagtat ctttctccca
    14641 ccccctcgtg atcacacccc gccaccagtg ctgtactggc tttccggatt aacctgcaat
    14701 gacgagaact tcaccaccaa ggcgggtgcc cagcgcgtgg cggcagaact ggggattgta
    14761 ctggtgatgc cagacaccag cccgcgcggc gaaaaggttg ccaacgacga tggctacgat
    14821 ttaggccagg gcgcaggctt ttatcttaat gccacgcaac cgccgtgggc gacgcattac
    14881 cggatgtatg attatctgcg cgatgaatta ccggcgctgg ttcagtcgca atttaatgtc
    14941 agcgaccgct gcgccattag cggtcactca atgggtggtc acggtgcgct gattatggcg
    15001 ctgaaaaatc cgggtaaata caccagcgtt tcggcctttg cgccaattgt gaatccgtgc
    15061 agcgtcccgt ggggaatcaa agcgtttagc acctatttag gtgaggacaa aaatgcatgg
    15121 ctggaatggg atagttgcgc gctgatgtat gccagtaacg cgcaggatgc gatcccgacg
    15181 cttatcgatc agggcgataa tgatcagttt cttgccgacc agttgcaacc tgcggtattg
    15241 gcagaagccg cgcgccagaa agcgtggccg atgacgctgc gtattcagcc gggatatgat
    15301 cacagttact acttcatcgc ctcttttata gaggatcacc tgcgcttcca tgcgcagtat
    15361 ttactgaagt gaaagtccgc ccggttcgcc gggcatcttc tcatcagaag cgataatcca
    15421 ctgccataaa gtaacgacgt ccgtcttcgt tatagctgta gtcgtcacga ctgagatcct
    15481 tgtcgccaag gttcagcacg cctgcgcgca gtttgacgtc tttagtcacc tgccaggccg
    15541 cgccggtatt ccagatggta taaccgcccg gtgttttagc cgtcgcgctg tcggcgcgtt
    15601 tctgcccggt atagtgacca gaaacataga atgaccagtc ttccagcgcc agcggtttcc
    15661 agtccagcgt accgttagca gtatggaacg gcagatcgga tagcggtttg ttttcgccgt
    15721 tgctgacatc acgaccatcg ttgtaggtgt agttgatcga cagtttccat tcatcgttga
    15781 acggaatttt cagttcggtt tccacgccct gaatacgagc tttgttaacg ttgtagtagc
    15841 taaataccgg tatgcgccgt ccgttagcgc ccgtctcaaa accaacaaag ttttggtagc
    15901 ccggtgcagc gttgacgtca gacgtacggc tgatgctgat acgatctttc acatcgttac
    15961 ggaaaacggt aacgctggat tcaacccctt ccagccagcc ttcttcaccc atgtagtaaa
    16021 gccccagctc ccaactttcg ctggtttctg gtttcagatc cgggctaccc acaatcttac
    16081 atgcgccacg gcaggaattg ctcgtccagt cagggctaag ttgcaacaga gaaggtgctt
    16141 taaatgccgt cgcccagccc cctttcaccg ttacggtgtc ggtggcgtta taaaccaggt
    16201 aggcacgcgg actccagtgt tcaccgtagg tttcgtgatc gtccatacgc acgccggtcg
    16261 tcagcgccag cggctcgaag atccgccatt catcttccac aaacagcgcg tactggctgg
    16321 cagacgtttt ggagctggtt cccccggtca ggttcaccgc atcgctaagt ttgtcgtgac
    16381 gccattcacc gccaaccgtg agaaactgat taatcgccgt cagcggcaac gtgtatttgc
    16441 cgtcgaccgt attgctttcg gaagttatcg ggctgctgtt gccagggttt ttgttctcga
    16501 ctttctcacc gtagtatttc agttcgctgg tgccgtaatc ccaacgccca ttatggctga
    16561 cggagtagtt ctggcgttcc aggcggtttt tgtccagcga gtcggaatca cgatcctgac
    16621 ggtcgaaacc gtatccggca gtaaaatcgt gattttgatt cggtgtccag gcaaattcga
    16681 cattgccgtc gcggctggag aatccttcaa tacgcggcgt ttctccggta tcggtggtcg
    16741 ttgagttttg cgggtcatcc ttttcacgtt ttgccaggct gccgtaagct ttcattccca
    16801 gcacaccatc aattaatggt ccactggtaa agaactgacc gttataggta tcaccgcgat
    16861 cgcgatgttc ctgaatggtg gtatcgacgg taacggtacc cgaccatttc tgaccgattt
    16921 ttttggtgat gatattcact acaccgccga gcgcatcgga accgtacagc gacgacatcg
    16981 ggccacggac cacttcaata cgttcgatgg aatcgaccgg gatccagttc agatcgaaat
    17041 cattgtggcg gaagacggca ttgcgggagt tcacgcgttt accgtcgacg agaatcaggg
    17101 tatagctgct gtccagacca cgaatactaa cgcccttacg gttatcccct tcgttcgtca
    17161 gttgtacgcc aggcacttct ttgaggacat ccttcagatt ctgtaccggt tttcgctgca
    17221 gatcttcctg ggtaatgacg ctgatactgg cgggtgcatc tttaaggttt tgttcaacgg
    17281 aagatgcagt gacaaccatc gtttcgccat cgtcatcgac cgctaacaca ggccatacac
    17341 aagaaatagc ggacaaacac agcccgaccc gtacgaaagg gttcaaccta aacattccat
    17401 atctccatga ggtaactacg aaaataaaat gggtatcgct cacatcttct ttacgtcccc
    17461 ttgcgtacgg cagcatcgcg gtggacttat ttgtatgcag gtgatcatcc cagaaagccg
    17521 gaccagcctc tgatttgtca gccttttttg attgtagcgt aacgataatg caaacgataa
    17581 caattatcaa tccaaatgtt aaattttata tccgcatgat ttgtcaggga aatggaattg
    17641 aaaaagccct ctcggttgag agggcttagc aaggaaggga ggaaacttat ttcttatcgt
    17701 tctgcgggaa cttcatttcg ctgtagcgta cgaagtgagt tcctttaatc agcttgtagc
    17761 cgaaccaaat aatcaggaac agcgggatac caatatacgt tgccgctacg ccgccccagt
    17821 caatggtatc tttcaggaac gcttcgtagt tctggcccaa agtgataatc agacacagaa
    17881 tgaatgcgaa gatcggcccc agtgggaaga aacctgaacg gtacggcaga tcgttaatgt
    17941 cgtgtccctg caatacgtaa ccgcgacgga agcgatagtg gctaatggca atccccagcc
    18001 aggcgataaa acccgtcatc ccggaggtgt tcagcagcca caggtatacc gtctggttgc
    18061 caaacatgga ggtcaggaag cacaaaccgg caatcaccgt cgtcgcatac agcgcattac
    18121 gcggcacgcc accacgcgac aatttagcga aaatgcgcgg cgctttaccg tcacacgcca
    18181 gggtgtacag catacgagta gacgcataca taccggagtt acctgctgac agcaccgccg
    18241 tcagaataac tgcgttcatc accgccgccg cagagagcag acccgcgtgc tggaacacca
    18301 aggtgaacgg actgacgctg atgtctttaa catcgttacg cagcaggctc ggatcggtat
    18361 acggaataat caggctgata atcaggatcg cgaacacata gaacaacagg attcgccaga
    18421 atacctgacg taccgcgcgt ggaatgtttt tcgccggatc ttcggactcg cctgcagcaa
    18481 taccgatcag ctcggttccc tggaaagaga agccgacaat catagctacg ccgatcatcg
    18541 ccgcaaaacc accagcaaac ggcgcttcgc cgattgtcca gttgctccag cccgcaggct
    18601 gcgcgccttt gaagataccg ataatcatca gcacgccaac gatgataaag acaataactg
    18661 tcgtgacttt gatcagtgaa aaccagtatt ccgcttcacc aaagccacga actgagatgt
    18721 agttcagcag gaagataacg ccgaggaaca acgcactcca gatccagccc ggtgtatccg
    18781 ggaaccacca gctcatgacc agctgtgctg caaccaagtc aacggcaata gtcaccgccc
    18841 agttgtacca gtagttccag cccagcgcga agccaaagcc ttcttcaaca tagttctgac
    18901 cgtaagtggc aaacgaaccg gaaaccggca tatatgcagc caattcaccg agactggtca
    18961 tcaggaagta aaccatcagg ccaatcagca tatacgagag caatgccccg cccggacctg
    19021 cctgagaaat cgttgcgcca gaggcaacaa aaagacctgt accgatggaa ccgccaatgg
    19081 caatcatcgt caggtgacgc gcctttaatt cacggcgtaa gcccggcgct tctgtagttt
    19141 tagtttcgga acccatacaa aaatgctatc catcttaaaa atgaggcgcg attgtagcag
    19201 acgattggca atccttccgg cagaaataca cggttataag acaccttcat gatcgcccag
    19261 ggattataag taaagcagcc aatctctttt ctggcgaatg catgctgagg gcgctgcgtc
    19321 acgaaatcga cacagcacca gcatgttctt gtacagcacc ttaacgcggt acattcgcgg
    19381 gatcgcaata gtccagaaag cgccgtagcg cgttggaaag gtgtttttgc cgatgatgta
    19441 tacgccacaa cgtacgcatc aggcgcggga gagggaccgc aacttcactt aatgtgcctg
    19501 cctgcaattg atcttcaatc acacgtcgcg acaggcaact aattcccaac ccatgacgca
    19561 ccgcatgttt gattgcctcg gagttaccta attccatcgc catctcaaac ttcggtaaat
    19621 gtgacagcaa cagataatcg acaatctccc gcgtgccgga accgcgttca cgcaggatcc
    19681 acggcgcagc ggccagctgt tctaaggtga ccggaccacg agccaacggc gaagtcggcg
    19741 cggcgaaaac caccagctcg tcttccagcc acggttcaga aatgatttca gtgctgtggc
    19801 acggtccttc aataaagcca atatcaacgc ggaaatccag caccgcttgc atcacgtcct
    19861 ggctgttccc aacgctaagt tcaatcggca actgcggata atcatggcga taacgggcga
    19921 taactgcagg cagaatgtag ttaccgatgg tactactggc atagatacga atcgcgccgt
    19981 tgtcttcgcg aaacagttgt tcgatttcaa ccgcctgttc aagcaatgcc aacgcacgcg
    20041 gatagagcag ccgcccgtgt tcattaacaa ccagtctttt ccccacgcga tcaaacagtt
    20101 gcacgccaag ctgcccttcc aggtcggtca aggctgcgct cactgctgat tgcgacaacg
    20161 ccagcatcac cgacgcctgg gtggttgatc cacttttcaa tacttctgca aaaacttcca
    20221 actgccggag ggtgatgtgc atagtcgctt accacttata aagattaatt ataaatatat
    20281 aatcaatttt atttttaaac cagttagtcg taaccttatg cccggttaaa ggagagggtt
    20341 atgacgaata tcaccttaca gaaacaacat cgtacactgt ggcattttat tccggggtta
    20401 gccctgagtg cagttatcac cggggtcgcc ctgtggggtg gttccattcc cgcggttgcg
    20461 ggtgccgggt ttagtgccct caccctcgca atcttgttgg ggatggtttt aggcaacacc
    20521 atctatccgc acatctggaa aagctgtgac ggtggtgtgc tgtttgccaa gcaatattta
    20581 ttacgtctgg gtatcattct ttatggcttc cgtctgacgt tctcgcaaat tgccgatgtc
    20641 ggtatcagtg ggatcatcat tgacgtgttg acgctgtcca gtaccttcct gctggcttgc
    20701 ttcctggggc agaaagtgtt tggtctggat aagcacacca gctggttgat cggtgccggt
    20761 agcagtatct gtggtgctgc cgcggtactg gcgactgagt cggtagtgaa agcggaagcc
    20821 agtaaagtaa ccgtggctgt tgcaaccgtt gttatcttcg ggaccgtcgc gattttcctc
    20881 tacccggcga tatatccgct gatgtctcaa tggtttagtc cggaaacttt cggtatctac
    20941 attggttcta ctgtgcacga agtggcgcag gtggtggcgg caggtcatgc catcagcccg
    21001 gatgcggaaa acgcagcagt tatttccaaa atgctgcgcg tgatgatgct ggctccgttc
    21061 ctcatcctgc tggcggcgcg tgttaaacag ctgtctgggg cgaacagcgg cgagaaaagc
    21121 aaaatcacta ttccgtggtt tgccatcttg ttcatcgtcg ttgccatctt taactcgttc
    21181 cacctgttac cgcagagcgt ggtgaacatg ctggtaacgc tggatacctt cctgctggca
    21241 atggcgatgg cggcgctggg tctgaccact cacgtcagcg cgctgaaaaa agctggggcg
    21301 aaacctctgc tgatggcact ggtactgttt gcctggctga ttgttggtgg tggtgcgatt
    21361 aactatgtga ttcaaagcgt catcgcataa accactacat cttgctcctg ttaacccgct
    21421 atcattaccg ttttcctcca gcgggtttaa caggagtcct cgcatgaaat acattggagc
    21481 gcacgttagt gctgctggcg gtctggcaaa tgccgcaatt cgcgccgtcg aaatcgacgc
    21541 aaccgcgttt gccttgttca ccaaaaacca acgtcagtgg cgtgccgcac cgctcacgac
    21601 gcaaaccatc gatgaattca aagccgcctg tgaaaaatat cactacacat cggcgcaaat
    21661 tcttccccac gacagttatc tgattaacct cggacatccg gtcactgaag ctctggaaaa
    21721 atcgcgcgat gcctttatag atgaaatgca gcgttgcgaa caactggggc tttctttgct
    21781 caacttccac cctggcagcc atctgatgca gatttcagaa gaggattgcc ttgcgcgtat
    21841 tgccgaatcc atcaacattg cgctggataa aactcaaggt gtaacagcgg tgattgaaaa
    21901 caccgccggt cagggcagta acttagggtt taaattcgaa catctcgcgg cgattatcga
    21961 cggcgtggaa gataaattcc gcgtcggcgt ctgcattgat acctgccatg ctttcgctgc
    22021 cgggtatgat ttgcgtactc cagccgaatg cgaaaaaacg ttcgctgatt ttgcccgtat
    22081 tgtcggcttt aagtatctgc gcgggatgca ccttaacgat gcgaaaagta cctttggcag
    22141 ccgcgttgac cgccatcata gcctcggtga aggcaatatc ggtcatgatg cgttccgctg
    22201 gatcatgcaa gacgaccgtt tcgacggcat tccgctgatc cttgaaacca tcaacccgga
    22261 tatctgggca gaagagatcg cctggctgaa agcgcaacaa actgaaaaag cggtggcctg
    22321 atcgatgaac gacagggaaa agcagattct taaaatttta cggcgtaacc cgctgattca
    22381 acagaatgaa atcgccgata ttctgcaaat cagccgctca cgtgttgccg ctcatattat
    22441 ggatttgatg cgcaagggtt tgattaaagg caaaggatat atccttaccg agcaggatta
    22501 ctgtgtggtt gtgggtgcaa tcaatatgga tattcgcgga atggcggata tccgttaccc
    22561 gcaagcagcc tctcatccag gttctgttca ctgttccgca ggcggcgtcg gacgtaatat
    22621 cgcgcataac ctggctctgc ttggtcggga tgtgcattta atatcagcta ttggcaacga
    22681 tttttacggc gagacattac tggaagaaac gcgccgcgcg ggcgtgaacg tttcaaactg
    22741 cattcgtctg catggacaca gcactgcaac ttatctcgct atcgctaata agcaggaaga
    22801 aactatcctg gcaatcaatg acacacatat tctgcaacag ctaacgcctc aactgctcaa
    22861 tacttcacgg gacttgattc gccatgctgg tgtggtgctg gctgattgta atctcacacc
    22921 tgaagccctg gagtgggtct ttaccattgc tgatgaaatt ccgatgtttg tcgataccgt
    22981 ttccgaattt aaagcgaaca aagtaaaaaa ctggtacagc cggatccaca ctctgaaacc
    23041 aacacaaaat gaactggaaa ttttgtgggg gcaaccgatt aaagacgaca acgatcgcat
    23101 cagagctgta aattcgctgc atcaacaagg tgttaaacgc atttttgttt atctgaaaga
    23161 tgaatctgta ttttgcagcg ataaggatgg agaacaattt ttgctgaccg cgccagcgca
    23221 tacgacagta gacagttttg gtgctgacga tggttttatg gcgggcctgg tatatagctt
    23281 tcttgaagga tacagtttcc gcgacagcgc ccgttttgcg gtagcctgcg cggcaatttc
    23341 gcgcgccagc ggcagcttaa acaaccctac cctgtctgcc gataacgcgc tttcattagt
    23401 gccaatggtg taacaatgtt gccggattcg acgcttgacc gcatcttatc cggctcataa
    23461 attgcgcact acgctaaacc aataaagaat cctgcaatag tagcactcat caggttagaa
    23521 agtgtcgccg ccgccagcgc gcgtaaacca agttgggcga tttccggcgc acggtgtggc
    23581 gcaaccgcag aaaacgcccc caccaccacc ccgatagaac caaagttagc gaaaccgcac
    23641 aacgcgaaag aaataatcgc cacggtttta gcatccagag tgccacccgt ttgcagatat
    23701 ggcgagaaat tgagataagc gacaaattca ttgatcgcca gcttctgccc aatcaaactc
    23761 ccggcaagat ttgcatcact ccagtcaacc cccatcaccc acgccaatgg ggccaacagg
    23821 taacctaaaa tcgactccag cgaagcatgt tcaaaaccaa accagccgcc aacaccgccg
    23881 ataataccgt taatcaacgc aattattgca acaaatgcca taacaactgt cgctacaccg
    23941 gcggcgattt tcagcccggt cattgcccct gtcgcagcgg cttcaataat gctttttggc
    24001 ggtgtttcgg tgaaagagag gttattaaaa gaaacctgcg aagattccgt agccgggctt
    24061 aacaggcggg caaacaagat cccgccaggg atcgccatta acgatgccgc cagcaaatat
    24121 tccacaggta cgcccagtgc ggcgtaacca atcattgtcg aaccagcaat cgaggccatg
    24181 ccactacaaa tcgctgtaaa taattcattg cgattcagac gatcgataaa gggtttgacg
    24241 atcgccggaa tttcgttttg cccgaggaaa atggtggtga ccgcgacgaa tgactcgatc
    24301 ttgctgatat ttaaagcttt ctggaagata ccgccgagaa tgcgaattaa gatccccatc
    24361 acaccgatgt agtagagaat actcaccagt gcagtgacga agataattgc cggtaatacc
    24421 ctgaaaccaa agataaatcc tgcgccatca aataaggtat ccattttcgg tccgaccaga
    24481 gaaccgaaga taaatgcgct acctgcgtcg ctgtacgcca tcactttgtg gacgccaaaa
    24541 gcgacttttt ctgcgaccca acgccctggc ggtaaccaaa gcataatccc gccaatcacg
    24601 acctgtaaca ctaacgccgc gccaacggta cgcaggctga tcttcttctt gtttactgac
    24661 agtaaaaacg caattgccag cagtaccacc attcccagaa cacttctcat gacatccata
    24721 gtgattatct cttcatgtca ttttgggcga cggctttctg cccatcagaa atagccgggc
    24781 ctgcatcttg cgatactgtc ccggcttgtt atcaacactt aatgggtttt tatgtagccg
    24841 cgtacgcact cttcgactaa tccccagaac cagtcggtat caatagtgat gccgacttta
    24901 gtattggcgg gcttgccaag aacgcccagc tcgtcgcaga cggtacgccc atagcaaggg
    24961 ccactgttga cgtccacttc gacgtacatc tcctgggttt taatgccatc agggttaatc
    25021 agataaccga tgcaggtggc gtcgtgcacc gggccgccag ccaggccgta gttttcgaac
    25081 tgcgttttga gagtgaagtt catgatgtcg ctgaacatct ctccggcagg gccgcctgcc
    25141 ctttccattc gtgcaattac gtccggggtg caaacggtct ggttggtaag atcgaggccc
    25201 atcatcacta atggaacgcc ggaggtgaac accacgcgtg cggcttccgg gtcggcaaag
    25261 atattgaact cggcagatgg ggtgaagttg cctgtaccgt aagcgccgcc cattagcaca
    25321 atttcacgga ttttgggcag gattgcgggt tgcatacgca ttgccaccgc gatatttgaa
    25381 agcggaccaa ccggcaccag agtgatatcg ccatcgcttg ccataagggt atcaatgatg
    25441 tatttcaccg cgtgggtact ttctgcctgg cgggtcagcg gctcgaatac cgggccatcc
    25501 agtccggttt caccgtgaat attatcggca acgatttgtt gacgcataat gggttgcggc
    25561 atccccgcat aaaccggaac attaatctcc agtttctggc aaacattcag gccattaatt
    25621 aatgttttat caagcgtctg attacccgca acaatggtga tacctaataa atcaatcgcc
    25681 ggatgtttcg ccgccatcat tatagcaata gcatcatcat gacccggatc acaatccaga
    25741 ataatttttc tcttttccat tgtttatttc ctctgtttcc agttgcgtta ttttttctac
    25801 agcaacgaaa tttcgatcgg gccgatgatt gaatcttaac aacagcgtac gaatgctaaa
    25861 tatgagaaat ctcatagcgg ataaacatcg tgaaagaaat ccacaataat gatcttaagc
    25921 agcaattgat gagtgaatcc gcgtttaagg attgcttttt aacggatgtt tcagccgata
    25981 cgcggctgtt tcatttttta gcgcgtgact acattgtgca ggaagggcaa cagccgtcct
    26041 ggctgtttta cctgacgcga ggccgcgcca ggctttacgc cacgctagct aatggtcgcg
    26101 tgtcgctgat cgatttcttt gccgccccct gttttattgg cgagattgag ttaatcgaca
    26161 aagaccatga accgcgtgcg gtgcaggcta ttgaagagtg ttggtgcctt gcgctcccta
    26221 tgaaacatta ccgcccgctg ttattaaacg acacgctatt tttacgaaaa ctctgcgtca
    26281 ccttaagtca taaaaattat cgtaatattg tttctttaac tcagaatcaa tcatttccgt
    26341 tagtgaatcg cctggcagca tttatattac tctcgcagga aggtgatctt tatcacgaaa
    26401 agcatacgca agcggcagag tatttaggcg tttcttatcg acatctttta tatgttctcg
    26461 cgcaattcat tcacgacggt ttattaatta aaagcaagaa agggtatcta attaaaaaca
    26521 gaaagcagtt gtcaggactg gcgctggaga tggacccgga gaataaattc tccgggatga
    26581 tgcagtaaaa attattggca atagcacgat tgccggatgc aacgcttaat aaggcgtaca
    26641 tccggcaatg aaactcaagc taaaccaata aagaacccgg caatggtcgc gctcatcagg
    26701 ttggaaagcg tcgccgccgc cagcgcccgt aaaccaagct gggcgatttc cggcgcgcgt
    26761 tttggcgaaa tagccgaaaa cgctccaacg acaacaccga tagaaccaaa gtttgcaaac
    26821 ccacagaggg cgaacgagat aatggcaatg gtcttgacat ccagcgtgcc gctcgtttgc
    26881 agatagggcg agaaattgag ataagcaaca aactcattaa tcgccagttt ctgcccaatc
    26941 aaacttccgg caagatttgc atcactccag tccaccccca taatccatgc caacggggcc
    27001 agcacatagc cgaaaatgct ttccagagag acattggcaa aaccaaacca gccgccaact
    27061 ccaccaataa tgccgttgat cagcgcaatg atcgctacaa atgccattac caccgtcgcc
    27121 acaccggcgg cgattttcag cccggtcatt gcaccgttcg ccgcagcttc aataatgctt
    27181 tttggcggtg tttcgctaaa cgacagattt tcaaacgtaa cttgcgaagg ttccgtcgcc
    27241 ggactaagaa tacgtgcaaa caggataccg ccaggaatag ccatcagcga agcggctaac
    27301 aaatagtcaa tcggcacccc cattccggca tagccaatca tcatcgaacc cgcaatagac
    27361 gccatgccgc tacaaatagc cgtaaatagc tcgttgcgat tcatgcgatc aataaaaggt
    27421 ttaacgatcg ccgggatctc attttgcccg aggaaaatag tggtgactgc gacaaaagat
    27481 tcgattttgc tgatgttgag ggctttctga aaaatactgc caagaatacg aatcagcagc
    27541 cccattacgc caatgtagta cagcaagctg ataagcgcgg taacgaaaat aatcgccggg
    27601 agtacgcgaa aggcgaagat aaagcccgca ccgtcaaaca gaacatccat tttgggaccg
    27661 accagcgaac caaaaataaa ggcacttcct gcgtcactgt aggacatcac tttatggacg
    27721 cctaatgccg cctgctctac tgcccatttt cccgggggaa aatagagcat gatgccacca
    27781 atggcaattt gcagcagcag cgcagcgcca accgtgcgca agctgatgct ctttttattt
    27841 accgacaaca caaaggcaat tgccagcaat accaccatcc ccgcaacact tctcattata
    27901 tccataatga ttttcccttc atgccagtaa acccggcgtc agcgccaggt tttggtatgt
    27961 ttgatgagta cgggcgacgg ctttctgccc gccagaaatt aacccacgag acgctgatat
    28021 tctttggcaa tttcgctcgc cagaatggcg ttattaaaga ccagttgaat attggatttc
    28081 aggctatcac cgccggttag ctccgcaaca cgcgccagca ggaacggtgt actctctttc
    28141 ccaataacac cctgttcttc ggcttcagca acggcttgat caattgctgc attgatggtt
    28201 tcttctggca tcgcaaactg ttccgggatc gggttcgcta ccaccaggcc gccgttcaaa
    28261 ccggtttgcc acttcactgc catcgcgcga gcaatttcgc gggcactgtc aagacgaatg
    28321 ctgacttcaa atgggctggt tcggcagaaa aacgcaggca gcgcttttgt ctgataacca
    28381 attaacggta caccgaaggt ttctaaatat tctgttgtta atcctaaatc gagaatagat
    28441 tttgcccccg cacaaacaac agtgacattg gtatttgcca gttcctgtaa atcggcagaa
    28501 atatcgaagg tatgttccgc tccgcgatgc acaccaccaa tacctccggt tgcaaaaact
    28561 ttaatcccag ccagtgctgc aataatcatg gttgatgcaa cggtagtcgc gccatttttc
    28621 cctgcagcaa cgacaaaagg taaatcacga cgactaactt tcgtcacgtt atgtccttca
    28681 cggcccagca attcgatttc ttctttactt aagcccactt tcatcacacc gccaataatg
    28741 gcgatagtgg ctggtactgc gccctgttta cgaattgttt cttctacttc aattgcggtt
    28801 tgggcatttt gtgggaatgg cattccgtga gaaattatgg tcgattccag cgcaacaacc
    28861 ggttttttat tttttaaagc atcctgtact tcagcggata tttgtaataa ttcaggggac
    28921 agagttaatt cagacattct gtgttctcca ctaacgatct aacattggca attgataatt
    28981 cagggttgtt ggtatattca caggcaagtg ccattgacga acatccctgc gcaaatcgaa
    29041 cagaatcgat aaacggcata ccgtctaccc aacaggaggc cagccccgcc atcatggcgt
    29101 cgcctgcccc agtaacattg atgacattcg ttttaatagg ggccgaccac ccactttcac
    29161 cgttgatatc actgtagtaa acgccatccc ctcccatgct cagcaccaag cggttcaggc
    29221 cgtgttgatg aaaccaggcg gcgacctttg cgacatcttc acgtccggaa agtgcaattc
    29281 cgctcaacgt ttcggcttca agacggttag gttttaacgt atggattttg ctcagatgat
    29341 cgcggatttt gacgcatttc catgccgata ccggatcaat aaatacaggc gtttccccgc
    29401 tattttccag aacccagacc agcacctctt cactaaggtt gcaatccgcg acaataacct
    29461 ttgcaccgcg aataaactcc tgatgctggg caagaaattc tgcagaaata cagtcactaa
    29521 tactcatatc atttatcgcg acaagcattt ctcctgtatt atcgagcagt gataaatagc
    29581 tggatgtatt ctcacccgga acaattaagc atttatcgac ataaacaccg gattgattag
    29641 tttgcgccag taatgattgc ccataaaaat caccacctac tgcacttaaa agccaggcat
    29701 ttttccccag taacgccaga ttatgagcga tattccttcc cacgccacct ggggtaaatt
    29761 taatttttcc gggattcgaa tccgcataat tcaatggatg atgtgaatac ccagccacat
    29821 caatattcgc cgaaccaata attactatgt agtccttttc gcgcatagca cgccccctgg
    29881 caataaattg ccaacgaaga aattgggtaa aatagccatt acagtcaata agactattag
    29941 agcacgtgtt cagatttaaa cacatgttca taataagcat atttaacgta atgtaaatcg
    30001 atcagaaaaa acgaaatcgg ccagatatga aggcggtcac aaagtttgtc tatttatgca
    30061 gcaacaaaag agaaagttgt gatgtgtaac acacaatatg gcaggcaaga aaaagggcag
    30121 agatgtttct gccctccaga ttgttgacaa cgcgcgcttt gttcatcctg gatgtggcgt
    30181 aaacgcctta tccgacctac aaaagcgcaa aaaattcaat atattgcagg gttcatgtag
    30241 gtctgataag catagcgcat caggcaattt tgcgtttgtc atcatcgggg cagagatgtc
    30301 tcatccccga taatgaagat aaattatgcg tttttttcta ctatttgtgt ttccgggcgt
    30361 ttcaggaagg cataggccaa acccgccacc agcgtaccgg caataattgc taccaggtaa
    30421 cccaataccg gcgtaatagc gccagggatc agcagaacaa acagaccacc gtgtggtgcc
    30481 atcagtttcg caccaatcgc cattgagatt gcgccagtca gcgccccacc aacgatacag
    30541 cacggtagca cacgcatcgg atcacgagca gcaaacggaa ttgcaccttc cgaaataaag
    30601 cacagaccaa gtaccagagc ggctttgcca ccttcctgct gcgctttgtc gaatttgcga
    30661 cgcgccacca ttgttgccag acccattgcc agcggtggca ccatacctgc cgccataatc
    30721 gccgccatcg ggccataggt ttgagtactc agcagaccca caccgaatgc gtacgctgct
    30781 ttgtttaccg gaccgcccat gtcagtacac atcatgccac cgaggatcgc ccccagcaga
    30841 accgcattcg cagtccccat ggtctgcagc cagtgagtca acccatcgag aatgccagca
    30901 accggtttac cgattaggta gatcatcgcc agaccgacca ccagactgga aattagcgga
    30961 atgatcagga tcggtttcag cgcctccata ctctgtggca gtttcaattg cgtactgatt
    31021 aacttcgcaa tgtaaccagc caggaagcca gcaattatac cgccaataaa gccagaaccg
    31081 gtgctgaccg ccagcatacc gccaatcaga cccggtgtga ggcccggacg atcggcaatg
    31141 gagaaggcaa tataacctgc cagtaccggc accatcagcg caaaggctga accaccacca
    31201 atctgcatca gcgccgctgc caacgtaccc ggctctttaa acgcttcgat accaaaagca
    31261 aaagaaagcg cgatacacag accacctgca acaaccatcg gcagcatgta agagacgccc
    31321 gtcagcaagt gacggtaagc gcctgcactc tctttcttac cttcagtggt cgctgtttga
    31381 gctttgcccg ctggttcata cggcgttgct tcagcaaccg ctttatccag ttcctgcgca
    31441 gttttcttca gcgccagacc ggtagaggtg cgatacatcg gtttaccagc aaatttcgcc
    31501 agatccactt cgatatctgc cgccacaatc accagatccg ctgctgcgac ttcttcaggg
    31561 gtgattgcat tgcccgcgcc aacagaacca cgggtttcaa ctttcaccca ccagccacgt
    31621 tttttcgctt cggtttcaat ggcttcagcc gccataaagg tgtgtgctac gccagtcggg
    31681 caagcagtca ccgcaactac gcgtttcgga ccgctggcag caaccggtgc tgtcgcagcg
    31741 accggcgcag tgtaaggttt cgcatggcct ttggcttcac tcaggaacag ctcagggtgc
    31801 gcaactgccc gggaaatatc acccagccag acatttttac cgttcagcgc gctgtcattc
    31861 gggatggaat caccgagaac aatcgccatt tctgcgtcgt tcggattgtc gatgatttcc
    31921 agttttgctt ttcgcgccgc cgcgcccagc agggtcttcg ccatataggc gcgtgcctga
    31981 ccgagattag cgtcaataat cagcagcgtt ttcattatgc ctctcctgct gtcagttaaa
    32041 aggttgtaag tcgacgcgcg ccatcattgc ggccaactgc ggacgatcgg taatacccac
    32101 attgctttga cttaccgcca gggctgcaac agctgtcgcc agacgcagtg tgtgttcact
    32161 ggattcacgc atcagcaagc cataaatcag gccaccaacc atagaatccc ctgcgccaac
    32221 ggtgcttacg acatcgactg acggtggttt ggcgatccat tcgccggagg cattaaccca
    32281 aagcgcgcct tcggcaccca gtgaaataac aacatgcgca ataccttgtt cacgtagcgc
    32341 atgtgcagct tcaatcacat ctttcatttc aggcagttta cggcctgccc agatttccaa
    32401 ctcgcggcgg ttaggtttca ccagccacgg tgccgctttc aaacctgcta ctaacgcttc
    32461 acggctacta tcaaagataa tgcaaggaca ctgactacgc aggcgagtca tccagtcggt
    32521 gaacgcttcc gggctgacgc ctgacggtaa gcttccgctg acacagacca tatcgaactg
    32581 accgagccag ctcagagaat cagtcacaaa gcgttcccag tcggcgaggg tgacttcaaa
    32641 acccgagaag ttgaagtcgg tcacttcgcc gtctttttcc gtcagcttaa cgttaattcg
    32701 ggtgcgcccc tgtacaacct ggaaacggtt ggcaatgccc agctcgctga acagttgctg
    32761 aaaaccatcc tgattgtctt tacccaggaa gccgccaacg gtgacatcaa ttcccaggtc
    32821 ttttaatact ttggccacgt tgatgccttt acccgccgca tgcagaccgg tggttttcac
    32881 caggttcact tcgccgcgtt caatttccgg gcagaaacca acaaggtcat aagccggatt
    32941 aagggtgatc gtagcaacac gtctgctcat tatgcgccct ccccaagacc agcagcgata
    33001 gcgtcgccaa tggctttcag cgcctgttca gcatctgcac cctgggcggt aaagcgtagg
    33061 cgatgacctt tcttaacgcc aagtgccaca actttcatca gactacgtcc gtttgccggt
    33121 ttgccggtgc catcaaggtt tgtcacggta atatcactgt taaattgttt aatggtattg
    33181 accagcatgg tacctggacg agcatgcagg ccgtgttcat tgcgcaccac aaactccgcg
    33241 cttaacacgt cgtcggtcgg cgcatcatcg ctggtcagca gcgccagcaa cgttgccgca
    33301 tccgctttca gcaagcggtc agctttattg tcgagcaata aatcagcgag acgcttaaga
    33361 accgcgatgg gctgatcgtc attcatcgcc acactcacca gcatggctgc cgtttcgccg
    33421 tccacatcaa aagcatttgc cgcacggctt accgcaatcg cgctacgcag attgccttcg
    33481 gcgctatcgc tcagccagat accctgtccg agattcagcg gttgttcatt gatggctttg
    33541 gtgacgaaag tggcgtcaac tgcccccacc tctttcagac gcgcagcgtt cagcgcctga
    33601 agagtcagca gatcgctggc gacgatatcc agcgtcagca tttcgttgtc gagcttcagc
    33661 tgctcactct gcttttcgcc catcagtaat gcgcgaagtt cttctgctgt tgttgctgac
    33721 ttcagttgtt cagcaacgga atcatcgctc agtacgtggg tcagctggcg tagcaggccc
    33781 agatgttcat ccgagctggc agcaataccg attgccacgt acgctacctg accgtcaccc
    33841 caggtgacgc cttccgggaa ctgaaatacc tgaacgccgg ttttcagcac ctgatcgcgg
    33901 gtgtcggtag tgccgtgtgg aatagcaata ccattgccga ggaacgttga ggtttgctgt
    33961 tcgcgcgcca gcatgccatt gacgtagcct tctgctacat taccggcctg caccagcgcc
    34021 gcagcgacct ggcgaatcgc ctcttctttg tctccggcct tttcgcccgg atggatgtcc
    34081 tgtacggata actggaacat agttctcctc tcttgctgaa ttgaaacgat tcagcctcta
    34141 tgagaaaaaa agcgccaacc tggcttaggg ttaaagacaa gatcgcgctg aaacgtttca
    34201 agaaagcata atacttctgt ttcagcacgc aaggaaagcc gtaaagttat gagcaaaagt
    34261 ttgatgtgct gcacaatttt ttgtcatttt tcctaattgc tgatgggaaa atccgttatc
    34321 agcaatttca tttcagcagc gtcagcagcc aatcagcaac agtgaaatgg cattttgatt
    34381 attcgtggcg cttttgattt cgctgtctgt ttattttctg acaagcagcg taaactccgc
    34441 gtcttcctct tccagtgatc gacctgcatg cataactccc ccgcagtctc cagcgcgaaa
    34501 tcgtttgacc tgacctcgac ggcgttttta atcgttgcct ttctcaccgg tattgcgggt
    34561 gctctgcaaa ccccgacact cagtattttc cttaccgatg aagtacatgc ccgtccggcg
    34621 atggtgggat tcttctttac cggcagcgct gtcattggga ttctggtaag tcagtttctc
    34681 gctgggcgct ctgataagcg cggcgatcgc aaatcgctga ttgtcttttg ctgcctgtta
    34741 ggcgtgctgg cctgcaccct ttttgcctgg aatcgcaact actttgtttt gctattcgtt
    34801 ggcgtctttc ttagcagctt tggctcgacc gctaacccgc aaatgtttgc ccttgcccgt
    34861 gaacatgccg acaaaaccgg acgtgaggcg gtgatgttca gctctttttt acgcgctcag
    34921 gtttcactgg catgggtcat tggcccaccg ctggcttatg cgttagcgat gggtttcagc
    34981 tttacggtaa tgtatctgag cgcagcggta gcgtttattg tttgcggcgt gatggtgtgg
    35041 ctgtttttac cgtcgatgca aaaagagctt ccgctggcga ccggcacggt tgaagcgccg
    35101 cgccgtaacc gtcgcgatac gctactgctg tttgtcattt gtacattgat gtggggctcg
    35161 aacagcctgt acatcatcaa catgccgcta tttattatca acgaactgca tcttcccgag
    35221 aaactggccg atgtgatgat ggggaccgcc gccgggctgg aaatcccgac gatgttgatt
    35281 gccggatatt tcgccaaacg tctgggtaag cgtttcttaa tgcgcgttgc tgccgtgggt
    35341 ggcgtctgtt tttacgcagg aatgctgatg gcgcattcac ctgtcattct gttgggcttg
    35401 cagctgctaa atgctatttt tattggcatt ctgggcggca tcgggatgct ctattttcag
    35461 gatctgatgc ccggtcaggc gggttcagcc accacgctct ataccaacac ttcgcgcgtg
    35521 ggctggatca tcgcaggatc agtggcgggc atcgtcgccg aaatctggaa ttatcacgct
    35581 gtgttctggt ttgcgatggt gatgattatc gccactctgt tttgcttact gcggattaaa
    35641 gatgtttaag gcgcggtcag catttccaga tcgataagcc aggtcatggc ctgctgacgc
    35701 gagttaccgc acatttcagc gctggcttgt aagcctgcac agactttcgg tcgcagtggc
    35761 gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa tgcagggcgt attggcgggc
    35821 ttgccatctg gcataccggg aatagggctg gaaattgaag gggcagtgca acacgccccg
    35881 caacccggac ggcattccat acgttgttct cttaaggtta accaccggat tgatgagcgc
    35941 gcacagtacc accttttttg caccagcaaa agtgcgaata ccacttgccc gaaaggcccg
    36001 tcgcgagtac tttgtcgcga tatttttgac attttcgact acaggaattt ttcgatgcca
    36061 agagcgaacg aaattaaaaa aggtatggta ctgaattaca acggcaaact gctgctggtt
    36121 aaggatattg atattcagtc gcccactgcc cgcggtgccg ctacgctgta caaaatgcgt
    36181 ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt tcaaaggtga tgatatcgtt
    36241 gacaccgtga cgctgacccg ccgttacgtt gacttctcct atgtcgatgg caacgaatat
    36301 gtctttatgg ataaagaaga ctataccccg tataccttca ccaaagatca gattgaagaa
    36361 gagttgctgt ttatgccaga gggcggcatg ccggacatgc aggtgctgac ctgggacggt
    36421 caactgctgg cgcttgagct gccgcagacc gttgatctgg aaatcgttga aaccgcacca
    36481 ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg cgacattgag cactggtctg
    36541 gtgattcagg taccagaata cttaagcccg ggcgaaaaaa ttcgtatcca tatcgaagaa
    36601 cgccgttata tggggcgtgc tgactaactt cagccgcatg cagaaaaggg atagctcagg
    36661 ctgtcccttt tttaatttat tataccaatc ttctattttg cgcttcctga acaccttacg
    36721 ccctcaattt tcactcgttg attgatcgcc ctcacacttc atcgcattaa caatccagac
    36781 caatttcaat tgctgtcata taactttaca ctgtcattgt taatttatcg ttactaagac
    36841 gtgactccta tgaagacaat tgcctccgtt acgctcccgc atcatgtaca cgctccacgc
    36901 tacgatcgcc agcagttgca atcacgtatc gttcattttg gctttggcgc ctttcaccgc
    36961 gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc agggcggcga ctgggggatc
    37021 tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc agctccgcgc acagaaccat
    37081 ttatataccg tgctggagaa aggtgcggac ggcaatcagg tgataattgt cggtgccgtt
    37141 cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa ttattgagaa attttgcgag
    37201 ccacaggtgg caattgtttc cctgacgatt accgaaaaag gctattgtat tgacccggcc
    37261 accggtgcac tcgacaccag taatccgcgg attattcacg atctacaaac ccctgaagaa
    37321 cctcactccg caccgggtat tctcgtcgaa gcactgaaac gccgccgtga gcgcggcctt
    37381 acaccgttta ccgtgctctc ctgcgacaat attcccgaca atggccatgt ggtgaaaaac
    37441 gcggtgctgg gaatggcaga aaaatgttcg ccagaactcg ccgggtggat aaaaaaacac
    37501 gtcagttttc cgggaaccat ggtcgaccgc attgttccgg ctgcaaccga cgaatcactg
    37561 gcagaaatca gccagcatct gggggtaaat gatccctgcg cgattagctg cgaaccgttt
    37621 atccagtggg tggttgaaga taacttcatc gctgggcgtc ctgcctggga agtcgcaggt
    37681 gtacaaatgg tgaatgatgt cctgccatgg gaagagatga aactgcgtat gcttaatggc
    37741 agccactctt ttctcgctta tctgggttac ctctcaggat tcgtccatat cagtgattgc
    37801 atgcaggatc gcgtatttcg ccatgctgcc agaacattaa tgctggatga gcaagcgccg
    37861 acactgcaaa ttaaagatgt cgatttaaca caatatgcgg ataagttaat tgcacgtttt
    37921 gctaatccgt cgctgaaaca taagacctgg caaatcgcga tggatggcag ccagaaatta
    37981 ccgcaacgta tgctggcagg tattcgcata catctggggc gtgaaacgga ctggtcgttg
    38041 ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg gcgttgatga tgccggaaat
    38101 gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg aacttgttgc gggcagcagc
    38161 agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag ttttcggtga tgatctgcca
    38221 gataacccgc attttgtgca ggccatcgaa caagcctggc aacaaatcgt acaattcggc
    38281 gcacatcagg cgctattaaa caccctcaaa atttaacgat ttctgcggtt aaagcggatg
    38341 aagctcacct tcgtccgctc tccccttctc ttttctgcct tttttagcca ggattaacgc
    38401 tcagttaact taccagagta ttgcggagcc gttgtgacca ggaccaacct catcaccggt
    38461 tttctcggca gcgggaaaac cacgtcgatt cttcatctgt tagcccataa agatcccaac
    38521 gaaaaatggg cggtactggt taatgaattt ggggaagtcg gaattgatgg tgctttgctc
    38581 gccgatagcg gcgcattgct gaaagagatc cccggcggct gcatgtgctg cgttaatggt
    38641 ttacccatgc aggtagggtt gaatacctta ctgcgtcagg gaaaaccaga ccgcttgttg
    38701 atagagccga ccgggctggg ccatccgaaa cagatcctcg atctgttaac cgcaccagtc
    38761 tatgaactgt ggatagatct gcgcgccacc ttgtgcattc tcgatccacg cctgctgctg
    38821 gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc tggctgccgc agacatcatt
    38881 gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc aagcgctaca gcgttggtgg
    38941 cagcaaaatg gtggcgatcg acaattaatt cacagtgagc atgggaaagt tgacggtcat
    39001 cttctggatt tgccgcgtcg caatttagcc gagttgcccg ccagcgccgc gcattctcat
    39061 cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc cagagcatca acgctggcgt
    39121 cgcagtctga acagcgggca aggatatcag gcctgcggct ggatattcga cgctgatacg
    39181 gtattcgaca ccattggcat tctggaatgg gcgcgacttg caccggtgga acgcgtcaaa
    39241 ggcgtgctgc gtattcccga agggctggta cgaatcaacc gtcagggcga tgacctgcac
    39301 attgaaacgc aaaacgttgc gccaccggac agccgtattg agctgatttc cagcagcgaa
    39361 gctgactgga atgctttgca gagcgcgctg ttgaagcttc gtttagcgac taccgcgtaa
    39421 ggttgcctgc gtttttcagt aagataatta gagaaaatat gattaaaaat ttgccgcaaa
    39481 tagtgttgtt gaatattgtc ggcctcgcgc tgtttctttc ctggtatatt cccgttaatc
    39541 atggattctg gttgccgatt gatgcggata ttttttattt ctttaatcag aaactggtcg
    39601 aaagtaaggc ctttttgtgg ctggttgcat tgaccaacaa tcgcgctttc gacggttgtt
    39661 ctctgctggc gatgggaatg ttgatgctga gtttctggct gaaagaaaac gcccctggca
    39721 gacgacgtat cgtgattatg ggtctggtca tgctattaac tgcagtggta ttaaaccagc
    39781 tgggtcaggc attaattcct gtaaaacggg ccagcccaac attgactttt accgatatta
    39841 accgcgtcag cgaactgctc tctgttccca cgaaagatgc ctcacgagat agctttcccg
    39901 gcgatcacgg catgatgctg cttatttttt cggcattcat gtggcgttat ttcggcaaag
    39961 ttgcaggcct tatcgccctt attatttttg tggtttttgc atttcccaga gtaatgattg
    40021 gcgcacactg gtttactgac atcattgtcg gttcgatgac cgtgatattg atcggtttgc
    40081 cctgggtgtt gctgacgcca ttaagtgatc gattaatcac cttttttgac aaatcactac
    40141 cgggaaaaaa caaacatttc caaaacaaat aactcacagt aattaacatc atcaggctta
    40201 tttttatagt gaggataatc ctgatgatgc gcaccgtgct ttcatccatc gcgcgcaaaa
    40261 atcattctct aagtaaatga atggattgca tgcgtttcac tcaatcgtac tttaattgac
    40321 caatcccgct tattaacttt ctgtatcact ttttcttata aaaaatcatg taaaaccgct
    40381 cgccaagacc gcaccaatcg ggtaatctag aactcgtttt gcctcggcgg tagattatcc
    40441 tcacagcata taattttgtg cgttagtcca cagatttggc cttaaggaat tgtttcaaca
    40501 tgcccaggta attagtctcg tgtcgcttgg cattttttta taacgatatt tgtcgttaag
    40561 gacttcaagg gaaaacaaac aacatggtca aatctcaacc gattttgaga tatatcttgc
    40621 gcgggatccc cgcgattgca gtagcggttc tgctttctgc atgtagtgca aataacaccg
    40681 caaagaatat gcatcctgag acacgtgcag tgggtagtga aacatcatca ctgcaagctt
    40741 ctcaggatga atttgaaaac ctggttcgta atgtcgacgt aaaatcgcga attatggatc
    40801 agtatgctga ctggaaaggc gtacgttatc gtctgggcgg cagcactaaa aaaggtatcg
    40861 attgttctgg ttttgtacaa cgtacattcc gtgagcaatt tggcttagaa cttccgcgtt
    40921 cgacttacga acagcaggaa atgggtaaat ctgtttcccg cagtaatttg cgtacgggtg
    40981 atttagttct gttccgtgcc ggttcaacgg gacgccatgt cggtatttat atcggcaaca
    41041 atcagtttgt ccatgcttcc accagcagtg gtgttattat ttccagcatg aatgaaccgt
    41101 actggaagaa gcgttacaac gaagcacgcc gggttctcag ccgcagctaa taaaccgttt
    41161 ggatgcaatc ccttggctat cctgacgagt taactgaaag cactgcttag gcagtgcttt
    41221 tttgttttca ttcatcagag aaaatgatgt ttccgcgtct tgatccaggc tatagtccgg
    41281 tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat taacggaatc atgttcatac
    41341 gcgctcccaa ttctggacgt aagctcctgc ttacctgcat tgtagcaggc gtgatgattg
    41401 cgatactggt tagctgcctt cagtttttag tggcctggca taagcacgaa gtcaaatacg
    41461 acacactgat tatcgacgta caaaagtatc tcgataccta ttttgccgac ctgaaatcca
    41521 ctactgaccg gcttcagccg ctgaccttag atacctgcca gcaggctaac cccgaactga
    41581 ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt gctggtgaaa gataaaaaaa
    41641 cattctgttc atctgcgact ggtgagatgg acattccact caatgaattg attccggcgc
    41701 tcaacattaa taaaaacgtc gatatggcga tcttacccgg cacgccgatg gtgccgaaca
    41761 aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa aaatagcggt gtgtttgccg
    41821 ctctgaatct caacctgacg ccttctctct tttatagttc acggcaggaa gattacgatg
    41881 gcgtcgccct cattattggc aatactgcgc tatctacctt ttcttcacgt ttgatgaacg
    41941 ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat tgcgggcatt cctctgaccg
    42001 ttcggcttta tgcggatgac tggacatgga acgatgtgtg gtacgcattt ttactgggcg
    42061 gcatgagtgg aactgtcgtt ggcctgctct gctattacct gatgagcgta cgtatgcgcc
    42121 ccggcagaga aatcatgacc gccatcaagc gcgaacaatt ttacgtggcg tatcaaccgg
    42181 tggtggatac acaagctttg cgagtaacgg gcctggaagt actgctacgc tggcggcatc
    42241 ctgtcgcggg agaaattccc ccggatgcct tcattaactt tgccgaatcg caaaagatga
    42301 ttgtgccgct gactcagcac ctgtttgagt taattgcccg cgatgccgca gaattagaaa
    42361 aagtgctgcc agtaggcgtc aaatttggta ttaacattgc gccggaccat ctgcacagcg
    42421 aaagctttaa agcagatatc cagaaactgc tcacttccct gcccgcacac catttccaga
    42481 ttgtgctgga aattaccgag cgcgatatgc tgaaagagca agaagccaca caactcttcg
    42541 cctggctgca ctcggtcggc gtagaaattg ctattgatga cttcggcacc gggcacagcg
    42601 cgcttatcta tcttgagcgt tttacgctcg attatctgaa aattgaccgt ggatttatca
    42661 acgccatcgg tacggaaacg atcacttcac ccgtacttga cgcggtgctg acgctggcga
    42721 aacgcctcaa tatgctgacg gttgctgaag gggtcgaaac gccggaacag gcgcgatggc
    42781 taagcgaacg cggcgttaat ttcatgcaag gctactggat tagtcgccct ttaccgctgg
    42841 acgattttgt ccgctggctg aagaaaccgt atacgccgca gtggtaaggt gtgcttacgt
    42901 cccttattat tcatagtgaa agcatgccgg attgcggcta atgatgagta aaaggaaatc
    42961 cattgcagat gattgtgcgc atactgctgc tgtttatcgc tctgttcacc tttggtgtgc
    43021 aggcgcaggc tatcaaggaa agctatgcct ttgccgtgct gggcgaaccc cggtacgcgt
    43081 ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc aaaaggtggg cagataacgt
    43141 tgtcagccct cggcaccttc gataatttca accgctatgc actgcgcggc aacccgggcg
    43201 cacgcaccga gcagctgtac gacacgctat ttacgacttc cgatgacgaa ccaggcagtt
    43261 attacccgct gattgctgaa agcgcacgct atgctgacga ttattcctgg gtggaggtcg
    43321 ctattaatcc gcgcgcccgt tttcatgatg gttcgcccat tactgcccgc gatgtagagt
    43381 ttacttttca aaaatttatg accgaaggcg tgccgcaatt tcgtctggtc tacaaaggca
    43441 ccaccgtcaa agccattgca ccgttaaccg tgcgcattga gttagctaaa cccagcaaag
    43501 aagatatgct gagtctgttt tcgctgccga tatttccaga aaagtactgg aaggatcaca
    43561 aacttagcga cccgctcgcc acgcctccgc ttgccagtgg tccgtaccgc gttacgtcct
    43621 ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga ttactgggca gcaaacttac
    43681 cggtaaaccg tggacgctgg aatttcgaca ccattcgcta cgattattac ctcgatgata
    43741 atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt gcgtatggaa aacgacgcta
    43801 aaaactgggc cacgcgttat accggtaaaa atttcgataa aaaatacatc atcaaagatg
    43861 agcaaaagaa cgaatcagcc caggatacgc gctggctggc gtttaatatc caacgtccgg
    43921 tattcagcga tcgccgggtc cgggaagcaa tcactctcgc ctttgacttt gaatggatga
    43981 acaaggcgtt gttttacaac gcctggagtc gcacgaacag ttattttcag aataccgaat
    44041 acgcggccag aaattacccc gacgccgcgg agctggtgct tctggcacca atgaaaaaag
    44101 atctaccgcc agaagtcttc acacaaatct accagccgcc ggtatctaaa ggcgatggct
    44161 acgatcgtga caacctgtta aaagccgaca aacttctcaa cgaaacgggc tgggtgctga
    44221 agggtcagca acgcgttaat gccacaacgg gtcagccgct cagctttgaa ttattgcttc
    44281 ccgcaagcag caatagtcag tgggtattgc cgttccagca cagcctgcaa cggctgggta
    44341 tcaatatgga cattcgcaag gtggataact cgcaaatcac taaccgcatg cgcagtcgcg
    44401 actatgacat gatgccgcgc ctatggcggg cgatgccgtg gcccagttcc gatttacaga
    44461 tttcctggtc atcggaatat atcaattcca cttataatgc ccccggcgtg caaagcccgg
    44521 ttatcgactc gctgatcaac caaattattg ccgcgcaggg aaataaagaa aaattactgc
    44581 cgttggggcg agcactggat cgcgtattaa cgtggaatta ttacatgctg ccaatgtggt
    44641 acatggcgga agaccgtctc gcctggtggg ataaattctc ccaacccgct gtacgccctg
    44701 tttacagcct gggtatcgat acctggtggt atgacgttaa taaagcggcc aaactgccgt
    44761 cagccagaca acagggagag tagatgggcg cttacctgat tcgccgtctg ttgctggtga
    44821 tcccaacgtt atgggctatt atcactatca actttttcat cgtgcaaatt gcgcctggcg
    44881 gtccggtcga tcaggccatc gccgccattg agtttggtaa tgccggagta ttacccggcg
    44941 caggcggtga aggtgttcgt gccagccatg cgcaaacggg tgtcggcaat atcagcgaca
    45001 gtaattaccg tggcggacgc ggattagacc cagaagtgat cgctgagatc actcatcgct
    45061 acggtttcga taagccgatc cacgaacgtt acttcaaaat gctctgggac tacatccgct
    45121 ttgattttgg tgatagcctg tttcgcagcg cctcggtgct gacgctgatt aaagacagtc
    45181 tgccggtttc catcaccctc ggattgtgga gcacgctgat tatctatctg gtgtcgattc
    45241 cgttaggcat tcgcaaagct gtttataatg ggagtcgctt tgacgtctgg agtagcgcat
    45301 ttatcatcat cggctacgcc attccggcct ttttgtttgc catcctgctg attgtcttct
    45361 tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg cctggtttcc gctaactttg
    45421 attcgctgcc gtggtatcag aaaatcaccg attatctgtg gcatatcacg ctaccggtgc
    45481 tggcgacagt gattggtggc tttgcggcgc tgaccatgct gaccaaaaac tcattccttg
    45541 atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa aggggtaagt gagaaaaaca
    45601 ttctctggaa acatgtgttc cgcaacgcca tgctgctggt gattgccggt tttccggcga
    45661 cgtttatcag catgtttttt accggctcgc tgctgattga ggtgatgttt tcactcaatg
    45721 gtcttggctt actgggctac gaagcgaccg tctcgcgcga ttatcctgta atgtttggta
    45781 ccttgtatat tttcaccctg attggcctgc tgctgaatat tgtcagtgat atcagctata
    45841 cgctggttga tccgcgtata gattttgagg gacgttaatg tcgcgactca gccccgtcaa
    45901 tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc tactggtcgt tatggatttt
    45961 cctcgttttg tttggtttga gtttgtgttc tgaacttatc gctaacgata aaccgttgct
    46021 ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa aactacagcg aaagcgattt
    46081 tggcggcccg ctggcaagtc aggctgatta tcaggacccg tggctgaaac aacggctgga
    46141 aaataacggc tgggtactgt gggcaccgat tcgctttggt gctaccagta tcaactttgc
    46201 taccgataag cccttccctt ctccaccctc ccggcaaaac tggctgggaa cggatgccaa
    46261 cggcggcgat gtgctggcac gcattctcta tggcacgcgg atctcggttc tgtttggcct
    46321 gatgctgact ctctgttcca gcgtgatggg cgtgctggcg ggggcgctac aaggctatta
    46381 cggcggtaaa gtcgatctct ggggacaacg ctttattgaa gtatggtcgg ggatgccgac
    46441 gctgtttttg attattttac tttccagcgt cgtacagcct aacttctggt ggctgctggc
    46501 aattactgtc ttgtttggct ggatgagtct ggtcggcgtg gtgcgggcgg agtttttacg
    46561 tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt ggcgtcagcg atcgcagtat
    46621 catcctgcgt catatgttgc ctaatgccat ggtcgcgacc ctcacctttt taccgtttat
    46681 tttatgtagt tcgataacca ccctgacctc gctcgatttc ctcggcttcg gtctaccgct
    46741 cggttcaccg tcactcggtg aactgctgtt acaagggaaa aataaccttc aggccccgtg
    46801 gcttgggatc accgccttct tgtcggtggc gatattattg tctttgctga tctttattgg
    46861 tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg tagcatgacg caaactctgt
    46921 tagcgattga aaatttgtcg gtgggttttc gccatcagca aaccgtacgt acagtagtca
    46981 atgatgtttc actacagatt gaggctggcg aaacgctggc gctggtgggt gagtcaggtt
    47041 caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct cccttccccg ccggttgaat
    47101 atctctccgg cgatattcgt tttcatggcg aatcgctgct tcatgccagc gatcaaacgt
    47161 tgcgcggtgt acgcggtaat aagatcgcca tgatttttca ggaaccgatg gtgtcgttaa
    47221 atccattgca taccctggaa aaacagcttt atgaagtgct ttcactccac cgcgggatgc
    47281 gtcgggaagc ggctcgtggc gaaattctta actgccttga tcgcgtcggt atccgccagg
    47341 cggcaaaacg gctgacagat tatccgcatc agctctccgg cggcgaacgc cagcgggtga
    47401 tgattgcgat ggcgctgtta acgcgaccgg aattattaat tgccgatgaa ccgaccaccg
    47461 cactggacgt ctctgtccag gcgcagattt tacagctgtt gcgcgaactg caaggcgagc
    47521 tgaatatggg catgctgttt attactcata acctcagcat tgtcagaaaa ctggcccacc
    47581 gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa taacgccgct acgctatttg
    47641 cctcacccac tcatccttac acacaaaagc tactcaacag tgaaccgtca ggcgatccgg
    47701 taccgttgcc agaacctgcc tcaacgttgc tggatgttga acagcttcag gttgccttcc
    47761 ccattcgcaa agggattttg aagcgcattg tggatcataa tgtggtggtg aaaaacatca
    47821 gttttacgct acgggcgggt gaaacactgg gtttagtggg cgagtctggt tccgggaaaa
    47881 gcacgacggg actggcgctg ctgcgactga ttacctctca gggcagcatc gtctttgacg
    47941 gtcagccact gcaagattta aatcgccgcc agctgttacc tactcgtcat cgtattcagg
    48001 tggtatttca ggatccaaac tcctcgctca acccacgact caacgttttg cagattattg
    48061 aggaaggctt acgggttcac cagccgacgc tttctgccgc acaacgcgaa caacaagtga
    48121 tagccgtgat gcatgaagtg ggattagatc ctgaaacacg ccaccgttat ccggcggagt
    48181 tctctggtgg tcagcgacaa cgtattgcga ttgccagggc attaattctt aagccctcac
    48241 tgatcatact tgatgaaccg acatcatcac tcgacaaaac ggtacaggcg caaatattga
    48301 cgctattgaa atcattgcaa caaaagcatc aactggccta tttgtttatc agccacgatt
    48361 tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact gcgacaaggg gaagtagtgg
    48421 aacaaggacc gtgcgcgcgc gtgtttgccg caccgcagca ggagtatacg cgtcagctac
    48481 tggcgttgag ctgacgctta aaaaggattg tagtttgaaa agggttcggc aattgccaca
    48541 ccaaaatttt tcaatcggca tgttgcagca aactcgtcct ggcgatttac aaacaggcac
    48601 ggctcacctt cacactccag caccgaacat ggaacttcga tgtcgcttaa cgcctggctt
    48661 agtttataca tcaccgacca tgcattatca ccatccgggc taagcaattt aagcgccacc
    48721 aggctgttgt caccgcacgg tccattttgc ggaatcggtt caaccttcga acctgcgaaa
    48781 ccagcagacc agcgaaagtt ctgcggtaga cgatggacga ttgagagttg taatgaagtc
    48841 acttattttc cccggaagca catttacttc acaatttatt tacatcaatt ttaacacatc
    48901 atcaacaatc cgtctttata cagatcgtaa agattgatgc ctcgcctgca tggcctctat
    48961 ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt gcggggctat atgtacccgt
    49021 ttctgcgatc taactcaacc tttttaacta caatgatgtg actttttaca taaattgatt
    49081 ttacataaaa taaacatata tcgggggaat gatagatttg tggttgacat gcatcaacaa
    49141 aggaagcctt ttagcttcct cgttgtgcaa tagatcaccg ttttttcggt cgactggcgt
    49201 acagacagaa gagaatggag ctggttgcgc agaatgcaat tgaccaaatc atcggccatg
    49261 cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat tgcccctatg ccaaaacgga
    49321 aggttcctgc cagcgaagat gccgttcccg ccatatgggg aaactcatca agaatgaccg
    49381 ccatcgcatt ggatgacacc atagacacgc agcccacaaa cgccgcaacg ccaaccacca
    49441 gcgaccagaa tcccagcccc agcagcgcac tgatgaccat ccacgctgcc ataataaatt
    49501 gtatccacaa ccccgagcgg aacatattta acgcgccaat gcggcggacg aagcggctgt
    49561 taaagatggt catcacgaac agaaaaacaa tgtttagcgc aaagtaataa ccaaagtttt
    49621 ccggtgcaac gtggttgatt tcaatgtaga caaacggtcc ggcgctcagg aacgagaaca
    49681 ttccggcaaa gctgaaaccg ctggccagca tataactcag gacacgctta tgacggaaca
    49741 gcgccgcaaa gttaccaata gtggtacgaa tgtgaaaagg ctggcgagga tccagaagat
    49801 gtaatgccag ctcagccaca ccagcaccca gccgccaact atcggtgcca tcagcggtgc
    49861 aatggtcgtc accagcatga caaacgacat catccgcgag aactcttctt tcgggtaaat
    49921 atcacgcatc aaagcgttga taaccacgct ggccgcagcc gcagccagcc cgtggaagaa
    49981 acgcatcaca atcagctgat cgatggtttg tgccaacgca cacgccaccg cggcggcggc
    50041 aaacaccagc gtaccgccaa gaaccaccgg cttacgcccg aagctgtctg ccatcggccc
    50101 gtagattaac tgccccaacg caaagcccag aatataagta ctcagggtca tctgcgtact
    50161 gcccgccggt acgccaaact gcgctgaaat taccggtagc gcgggcagat acatatcaat
    50221 cgacagcggc atcaacatgg ccagcaggcc aaggataaaa acaatagcaa acgacgaatg
    50281 ctgtcgggtg gtcacaacgg gctcctgaaa gtcattgaaa agttagacga cgctggcaat
    50341 ttcttcttca gttaacggac gatattcacc gggggctaaa tcagcatcca gcgtaatgcc
    50401 gccaatacgt tcacgatgca gctcaaccac atggttaccc acggcggcga acatgcgttt
    50461 cacctgatga taacgccctt cgctgatggt cagacgaacc tgcgttgggg taatcacttc
    50521 cagcaccgcc ggcttagtga gatctttttc gttatgcagc tgcacgcctt tagcaaattg
    50581 ttctgccgta tcgtcagcta cgggtgatac cagcgtcacc agataggtct tctcgcaatg
    50641 atggcgcgga gaagtaatgc ggtgcgacca ctgaccatca tctgtcatta gtaccagacc
    50701 ggtggtatcg atatccagcc gccctgcggc gtgcagtttc cacgctaccg gttcatctag
    50761 aaaatagagc accgttgggt gatcagggtc gtccgtggag caaacataac cctgaggctt
    50821 attgagcatg aagtaacgtg gaccgtgttg ctgcgccagc gggttgccat cgtaagcgac
    50881 atcatgttca ggaagcagtt tgaacgctgc attacggacg atttcgccat cgacggtgac
    50941 acgattgccg cggatttcac gcccggcaat agcacggcta acgccgagtt gctgtgcgat
    51001 aaatttatca agtcgcatgt gtgtgatttt gcctgtaaaa aacggaggtc gggtaatgac
    51061 ccgaatatct gaactgttgt ctgcccagta tagcggtcta ataacatccc tcaagggaaa
    51121 aagatccgtg gcatactatt agcagaataa tctacctacg cgagaccatg atttttacac
    51181 ttcgcccata tcagcaagaa gccgtggatg ccacgctcaa ccattttcgt cgtcataaaa
    51241 cccctgccgt tatcgtgctg cccaccggtg caggtaaaag cctggtgata gcggaactgg
    51301 cacggctggc tcgtggtcgc gtactggtgc tggcacacgt taaagaactg gtggcgcaaa
    51361 accatgcaaa gtatcaggcg ctggggctgg aagccgatat ttttgccgcc gggctgaagc
    51421 gtaaagagag ccacggtaaa gtggtatttg gcagtgtgca gtcggtcgcc cgtaatattg
    51481 atgcctttca gggtgaattt tcgctgttga ttgtcgatga atgtcaccgt attggtgacg
    51541 atgaagagag ccagtatcag caaatcctca ctcacctgac aaaagtgaat ccccacttac
    51601 gcctgctggg gctgactgcc acgccttttc gattgggcaa aggttggatc taccagttcc
    51661 attatcacgg catggtacgc agcgatgaga aagccctttt ccgtgactgc atttatgagc
    51721 tgcccctgcg ttatatgatt aaacacagct atctgacgcc gccagaacga ctggatatgc
    51781 cagtagtgca atatgatttc agccgcttgc aggcacagag caacgggctg ttcagcgaag
    51841 ccgatctcaa ccgtgagctg aaaaaacagc aacgtattac cccgcacatc atcagccaga
    51901 ttatggagtt cgccgaaaag cgtaaaggag tgatgatttt cgccgcgacg gtcgaacacg
    51961 caaaagagat tgtgggatta ctgcctgccg aagatgcagc actgattact ggcgacaccc
    52021 ccggcgctga gcgcgatgtg ttaattgaaa attttaaagc ccagcgtttt cgctatctgg
    52081 tcaacgtcgc ggtactgacc accggatttg acgccccgca cgtcgatctt atcgccattc
    52141 tgcgccctac cgaatcagtg agtctttacc aacaaattgt cgggcgcggt ctgcgtctcg
    52201 ctccgggcaa gactgattgt ttaattcttg attatgcggg taatcctcac gatctctacg
    52261 cgccggaagt tggtacaccg aaaggcaaaa gtgacaacgt tccggtacag gttttctgcc
    52321 ctgcctgcgg ttttgccaac accttttggg ggaaaacgac cgccgacggg acattgattg
    52381 aacactttgg tcgccgctgt cagggatggt ttgaagatga cgacggtcat cgcgaacagt
    52441 gtgacttccg tttccgtttt aaaaattgcc cgcaatgtaa cgcagaaaat gatattgccg
    52501 ctcgccgctg ccgcgaatgt gacacggtac tggttgatcc ggatgatatg ttaaaagcgg
    52561 cgctacgact gaaagatgcg ctggtattac gctgtagcgg catgtctttg cagcatgggc
    52621 acgacgagaa aggcgaatgg ttgaaaatca cctattacga tgaagacggt gcggatgtga
    52681 gtgagcgttt ccgtctgcaa acacctgccc agcgtaccgc cttcgagcag ctttttatcc
    52741 gcccgcatac gcgcacaccg ggcatcccgc tgcgctggat caccgccgcc gatatcctcg
    52801 cccagcaagc cttattgcga cccccggatt ttgtcgtcgc ccgcatgaaa ggccagtact
    52861 ggcaggtgcg tgaaaaagtg ttcgattacg aaggtcgttt tcgtcgggcg cacgaattac
    52921 gcggttaatg gtagttttga ttgatgtata aggcgattga gtatagaatc tcacccgctt
    52981 ttgcatacgc aaagcagatc acttacctgt tgctgggtcg cctgtagcag gattaattta
    53041 agagagaaag aaatgtttac tatcaacgca gaagtacgta aagagcaggg taagggtgcg
    53101 agccgccgcc tgcgtgccgc taacaagttc ccggcaatca tctacggtgg caaagaagcg
    53161 ccgctggcta tcgagctgga tcacgacaaa gtcatgaaca tgcaagctaa agctgaattc
    53221 tacagcgaag ttctgaccat cgttgttgac ggtaaagaaa tcaaagttaa agctcaggac
    53281 gtacagcgtc acccgtacaa accgaagctg cagcacatcg acttcgttcg cgcttaattg
    53341 ctgaataagt tgtaaaaaac cccgctcccg cggggttttt tgtatctgca gattatgcct
    53401 gattacggta ttgctatttt ttgcaggcca gataaggcgt tcacgccgca tctggcattc
    53461 agaacacaaa accgattaat tgccgccaga cgtccggcgc tgcaattggt cgcgcaaatt
    53521 cggcggcgtg cctttaatgg tcagggtatc ggtcgccggc tcccagaaga tgcgttcacc
    53581 taacagcatc gcatcaaagt taatagtcaa gccaccgccg ctaccggcaa acttcgtcaa
    53641 ctgacgcagt gtgctccggt ctgccgggaa gctctcttcc agttcgtagc ctttttcagc
    53701 cgcaaactcc gtgaagctca cttcgcttac gcctgccagc tctttcgaca gcgattccag
    53761 ttcgatctct tcgcctgcct gcagctgctc attacagtag ctataaacct gctggcgcac
    53821 gttctgtcgc tctgctttat ccagttgtgc ttcggcagtg aaatcatcaa ccgcttgcaa
    53881 cagcccacgg ttttgtgctt tcgtgttcaa tccttcgctg gccccgagga aatccataaa
    53941 gaaatccgcc actttgcgcc ctactcgccc tttcaggaaa gtgagatagc gggtggactc
    54001 tggattggtt tcccattcgg ttaaatcgat acgcgcaaca atatccgcat gattgatatc
    54061 aagataatgg gttgggttga tgtcgagatt ttcgttaaca cgcatactgc tcaggttgct
    54121 cagaactgcc accagcagat actccaccgc cagataacgg tagtggcaaa acagcacgaa
    54181 tccaccgtca gcaaaaggat atttcgctaa ttcgtcacgc aggcgaccag ttgccgcgcg
    54241 gctaaatgcc agaaagtcct cttcgccctg acgctgtaag cgcagcgtct gcgccagttc
    54301 actctcttcg ctaaacaagc cgtaagcttt atttttggcg ctatagaccc gatgcagttc
    54361 tgccaccatc tcaacgacgg tttctgtcgg ttccagcaat gaatcgcgca acaccagctc
    54421 aaggttctgc tcatcacgct tgataagctg gtgcagagca atctggttga tatccagact
    54481 catgataaac tctcctttaa gaccgggcgg tattcaacca ccgccggtta agcgacgcaa
    54541 taaaagatgg cggtcatttt acagaaggca gcgtaacgag aatgatttaa cgcataaaaa
    54601 agcagaaaaa aaaccgttgc tacggtaata tgttgccctt tcatcaacta actgatttcg
    54661 atttatgcca caaatttccc gctacagtga tgaacaggtt gaacaattgc tcgcagagct
    54721 gctcaacgta ctggaaaaac ataaggctcc gaccgacctt tccctgatgg tgttaggcaa
    54781 catggtcact aaccttatca acaccagcat tgccccggcc caacgccagg caattgccaa
    54841 ctcttttgcc cgcgccttac agtcctctat caacgaagac aaagcgcact aagggaaaca
    54901 gataacaggt tatggtaact catcgtcagc gctaccgtga aaaagtctcc cagatggtca
    54961 gttgggggca ctggtttgca ctgttcaata ttctgctttc gctcgtcatt ggcagccgtt
    55021 acctgtttat cgccgactgg ccgacaacgc ttgctggtcg catttattcc tacgtaagca
    55081 ttatcggcca tttcagcttc ctggtgttcg ccacctactt gctgatcctc ttcccgctga
    55141 cctttatcgt cggctcccag aggctgatga ggtttttgtc cgtcattctg gcaacggcgg
    55201 gaatgacgct attactgatc gatagcgaag tctttactcg tttccatctc catcttaatc
    55261 ccatcgtctg gcagttggtt atcaacccag acgaaaatga gatggcgcgc gactggcagc
    55321 tgatgttcat cagcgtgccg gttattttat tgcttgaact agtgtttgcg acgtggagct
    55381 ggcaaaagct gcgcagcctg acgcgtcgtc gacgcttcgc gcgcccgctg gccgcattct
    55441 tatttatcgc ctttatcgcc tcgcatgtgg tgtatatctg ggccgatgcc aacttctatc
    55501 gcccgatcac catgcagcgc gctaacctgc cgctttcgta cccgatgacg gcgcgacgtt
    55561 ttcttgagaa gcatggtctg cttgatgcgc aggagtatca acgccgtctt attgagcaag
    55621 gtaatccaga cgccgtttcc gttcagtatc cgttaagcga actgcgctat cgcgatatgg
    55681 gcaccgggca gaatgtgctg ttgattactg tcgatggcct gaactactca cgcttcgaga
    55741 agcagatgcc tgcgctggca ggttttgctg agcaaaatat ttcgttcacg cgccatatga
    55801 gctccggcaa cactacagac aacggcatct ttggcctgtt ctatggtatc tcgccgagct
    55861 atatggacgg cattctgtcg acccgtacgc ctgcggcatt aattactgcg cttaatcagc
    55921 aaggctatca gctggggtta ttctcatcag atggctttac cagcccgctg tatcgccagg
    55981 cattgttgtc agatttctcg atgccgagcg tacgcaccca atccgacgag cagaccgcca
    56041 cgcagtggat caactggctg ggccactacg cacaagaaga taaccgctgg ttctcgtggg
    56101 tttctttcaa tggcactaac attgacgaca gcaatcagca ggcatttgca cggaaatata
    56161 gccgggcggc aggcaatgtc gatgaccaga tcaaccgcgt gctcaatgca ctgcgtgatt
    56221 ctggcaaact ggacaatacg gttgtgatta tcactgccgg tcggggtatt ccgctgagcg
    56281 aagacgaaga aacctttgac tggtcccacg gtcatctgca ggtgccatta gtgattcact
    56341 ggccaggcac gccggcgcag cgtattaatg cgctgactga tcataccgat ctgatgacga
    56401 cgctgatgca acgcctgcta catgtcagca cacctgccag cgaatattcg caaggtcagg
    56461 atttgttcaa ccctcaacgc cgtcattact gggttactgc agcggataac gatacgctgg
    56521 caattaccac cccgaaaaag acgctggtgc tgaacaataa cggtaaatac cgcacttaca
    56581 acttacgtgg tgaaagagtg aaagatgaaa aaccacagtt aagtttgtta ttgcaagtgc
    56641 tgacagacga gaagcgtttt atcgctaact gattaattat aaatcagtta gcgaaatatc
    56701 ttacttgcaa tcggtgtgga aaacggtagt attagcagcc acgagtcggc acgtagcgca
    56761 gcctggtagc gcaccgtcat ggggtgtcgg gggtcggagg ttcaaatcct ctcgtgccga
    56821 ccaaaaatcc caagaaaaaa ccaaccttta cggttggttt ttttatatct gcaattaatt
    56881 tgataaacag accgtgacac atcacagcct gtttattttc tgttatcaga acgtccagac
    56941 cactcccgcc tgagtattcc acggcgattc tacccctgcg ccattgccat agctgactga
    57001 caaatgaccg cttaacgtcg gggtaaacga tgaccttatc ccagcctgat aaacgccacg
    57061 ggttccagcc acatcattat taaatttgcc atcgtcatta actttcacat ggttagaatc
    57121 ggcgtattcc tgacgcacgg cggctttcag ccagggttcc aacgtcgtac cgttttgcag
    57181 gtccatgtga tagcttaccg ccgttcccgc ttcagcgcgt aatatccggg tatttcccac
    57241 atccgcgcgc atgccgtttg ataacgtgta gtcctgacca tctgtggtaa agccggtaaa
    57301 ggcgagatag ggtctaacac tccacaatcc gtcaacccaa cggaacccgc tctcaacatg
    57361 agcacccgcg ccgttactat tgtaatcgcc aaacgctgtt gccccattac tcatcttgcc
    57421 atggatggtg ttggcaaaac ggtcaacttt caccacccca tcaacatagg caccgttctg
    57481 atgctcccaa ccggcataag cccccagggt gtagctatca atattgcctt tgccgccgcg
    57541 atcaaaacca atatcagaat gagagtaatc aaagaacaag ccgcgaattg tgctgctttc
    57601 ttcacgggag aaacggctat cgataccgag cgtcaggccc gtcaatgttt gctcaaagcc
    57661 agctcccgca tcagtggtca cgttgttgcg ggtgttaatt gccgaactcc acatcgccgt
    57721 atcgtaatta acgcctttta cgctaccaag acgctcacgc acggtgtcca gttctgcatc
    57781 aaataccagc ggttgtgctg ctgccatgtt cagcacatca gtggttgaag gggtaatttg
    57841 cgcgcgattc tctgcgaggc tccagctatg gttgccatta tccagcaagg tatattcata
    57901 cgtaccgata tcaacaacgc ctccggcatt gcccaacgtg aatgcagcat caccgccgcc
    57961 cgtcgttacc agtgtaaggc tatctcctgc tgccgggctg gcccccgtgt ccgtcacgaa
    58021 tattttgaaa tcacctgttg cctgaccgat gacgttgagc tgatcgctct gatgattagc
    58081 catatcggta cgcatataaa attgccgttt cccgacaggg tattggtggt caacgtaatg
    58141 aaattttccg cacttgccgt cacagatgaa cgagtcactg gctcagccat cataatcaca
    58201 tttgcgccgg acatcgcaag gttggctaag ttgaggcgag tgttttggtt cgtcgtagtg
    58261 atatcggcaa tgatggtcca tgcgctattt tcgagcgaaa cgtccgcttg gctggtatcc
    58321 acggcaccat gcgttctaaa agaagcgccc tcagaaacca ctaccttacc caccaacata
    58381 gcgggtgcat aatttatttt cggcttcatg acctcaagaa tgccatcatt ccctctgact
    58441 gacacgttaa ccattgtgcc agcccagaca ttagcgcggc cattgttgat aaccatattt
    58501 tcagagatgg ctttagagga atacgtgata cttccattct gatctgatcg accgagatca
    58561 tatacagcat ttgcctgcac tttcgtacca gtatcctttc ccagcgattg catagtggca
    58621 tgctcatcaa ggatagtgtc aatggcctga gtgtcctcca taacaatgag cccggagcca
    58681 tcatccagtt cataattttt tgacacacca tcctttatac taaattggcc tttactgttt
    58741 gtaccactca cttccagcgc attcgttgag acaattaatt tcccgcctgc ttttttatca
    58801 acgccagttg cagtcccgcc atccataacc tccagtaatc ctcccttttc gatagcggca
    58861 aagacggcaa tcccatcttt ttcaacaatt aagcgcccgc ctttaatcgt gggatcattc
    58921 gccgcgcctg gatttgcccc tatatttcca gacacctgtt atcacttaac ccattactgg
    58981 cttgctgccg tagatattcc cgtggcgagc gataacccag tgcactatgc ggatgccatt
    59041 cgttataatg ctcgaacgc
//
LOCUS       DAJTYZ010000008        58385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000008 DAJTYZ010000000
VERSION     DAJTYZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.008"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..160
                     /locus_tag="OO596_000469"
     CDS             <1..160
                     /locus_tag="OO596_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6716423.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(198..1259)
                     /locus_tag="OO596_000470"
     CDS             complement(198..1259)
                     /locus_tag="OO596_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6716424.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1472..2935
                     /gene="bepA"
                     /locus_tag="OO596_000471"
     CDS             1472..2935
                     /gene="bepA"
                     /locus_tag="OO596_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6716425.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2956..3315
                     /gene="arsC"
                     /locus_tag="OO596_000472"
     CDS             2956..3315
                     /gene="arsC"
                     /locus_tag="OO596_000472"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6716426.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3453..4199)
                     /gene="hda"
                     /locus_tag="OO596_000473"
     CDS             complement(3453..4199)
                     /gene="hda"
                     /locus_tag="OO596_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6716427.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="OO596_000474"
     CDS             complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="OO596_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6716428.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5624..6250)
                     /gene="upp"
                     /locus_tag="OO596_000475"
     CDS             complement(5624..6250)
                     /gene="upp"
                     /locus_tag="OO596_000475"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6716429.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6575..7612
                     /gene="purM"
                     /locus_tag="OO596_000476"
     CDS             6575..7612
                     /gene="purM"
                     /locus_tag="OO596_000476"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6716430.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7612..8250
                     /gene="purN"
                     /locus_tag="OO596_000477"
     CDS             7612..8250
                     /gene="purN"
                     /locus_tag="OO596_000477"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6716431.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8421..10487
                     /gene="ppk1"
                     /locus_tag="OO596_000478"
     CDS             8421..10487
                     /gene="ppk1"
                     /locus_tag="OO596_000478"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6716432.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10492..12033
                     /gene="ppx"
                     /locus_tag="OO596_000479"
     CDS             10492..12033
                     /gene="ppx"
                     /locus_tag="OO596_000479"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6716433.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="OO596_000480"
     CDS             complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="OO596_000480"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6716434.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14497..14649)
                     /locus_tag="OO596_000481"
     CDS             complement(14497..14649)
                     /locus_tag="OO596_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716435.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14667..14858
                     /locus_tag="OO596_000482"
     CDS             14667..14858
                     /locus_tag="OO596_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6716436.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15169..15687
                     /locus_tag="OO596_000483"
     CDS             15169..15687
                     /locus_tag="OO596_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6716437.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15703..16242
                     /locus_tag="OO596_000484"
     CDS             15703..16242
                     /locus_tag="OO596_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6716438.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="OO596_000485"
     CDS             complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="OO596_000485"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6716439.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="OO596_000486"
     CDS             complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="OO596_000486"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6716440.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19611..20981
                     /gene="xseA"
                     /locus_tag="OO596_000487"
     CDS             19611..20981
                     /gene="xseA"
                     /locus_tag="OO596_000487"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6716441.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20978..21193)
                     /locus_tag="OO596_000488"
     CDS             complement(20978..21193)
                     /locus_tag="OO596_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6716442.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21262..22734)
                     /gene="der"
                     /locus_tag="OO596_000489"
     CDS             complement(21262..22734)
                     /gene="der"
                     /locus_tag="OO596_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6716443.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="OO596_000490"
     CDS             complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="OO596_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6716444.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="OO596_000491"
     CDS             complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="OO596_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6716445.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="OO596_000492"
     CDS             complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="OO596_000492"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6716446.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="OO596_000493"
                     /gene_synonym="gcpE"
     CDS             complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="OO596_000493"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6716447.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="OO596_000494"
     CDS             complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="OO596_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6716448.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="OO596_000495"
     CDS             complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="OO596_000495"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6716449.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="OO596_000496"
     CDS             complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="OO596_000496"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6716450.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="OO596_000497"
     CDS             complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="OO596_000497"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6716451.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="OO596_000498"
     CDS             complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="OO596_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCR6716452.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37872..38717
                     /gene="sseA"
                     /locus_tag="OO596_000499"
     CDS             37872..38717
                     /gene="sseA"
                     /locus_tag="OO596_000499"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6716453.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="OO596_000500"
     CDS             complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="OO596_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6716454.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="OO596_000501"
     CDS             complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="OO596_000501"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6716455.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="OO596_000502"
     CDS             complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="OO596_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6716456.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="OO596_000503"
     CDS             complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="OO596_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6716457.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="OO596_000504"
     CDS             complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="OO596_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6716458.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTSVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="OO596_000505"
     CDS             complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="OO596_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6716459.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="OO596_000506"
     CDS             complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="OO596_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6716460.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="OO596_000507"
     CDS             complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="OO596_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6716461.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="OO596_000508"
     CDS             complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="OO596_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6716462.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="OO596_000509"
     CDS             complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="OO596_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6716463.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="OO596_000510"
     CDS             complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="OO596_000510"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6716464.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48470..49273
                     /gene="suhB"
                     /locus_tag="OO596_000511"
     CDS             48470..49273
                     /gene="suhB"
                     /locus_tag="OO596_000511"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6716465.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49418..50272
                     /locus_tag="OO596_000512"
     CDS             49418..50272
                     /locus_tag="OO596_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6716466.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50463..51743
                     /gene="csiE"
                     /locus_tag="OO596_000513"
     CDS             50463..51743
                     /gene="csiE"
                     /locus_tag="OO596_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6716467.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51735..52874)
                     /locus_tag="OO596_000514"
     CDS             complement(51735..52874)
                     /locus_tag="OO596_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6716468.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="OO596_000515"
                     /pseudo
     CDS             complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="OO596_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
     gene            54060..55421
                     /locus_tag="OO596_000516"
     CDS             54060..55421
                     /locus_tag="OO596_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6716469.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55418..55936
                     /locus_tag="OO596_000517"
     CDS             55418..55936
                     /locus_tag="OO596_000517"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6716470.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55936..56256
                     /locus_tag="OO596_000518"
     CDS             55936..56256
                     /locus_tag="OO596_000518"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6716471.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56253..57065
                     /gene="hcaB"
                     /locus_tag="OO596_000519"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56253..57065
                     /gene="hcaB"
                     /locus_tag="OO596_000519"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6716472.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57075..58277
                     /locus_tag="OO596_000520"
     CDS             57075..58277
                     /locus_tag="OO596_000520"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR6716473.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14271 a        15783 c        14566 g        13765 t
ORIGIN      
        1 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtaa gccattttat
       61 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
      121 tatgtgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
      181 gttcgtcggg ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg
      241 gctttgatca gcgtcgccaa tggaatagcg aagaatacgc cccagaatcc ccacaaacca
      301 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
      361 aacaccggta ctaacagatt accgtccagc gcctgaataa tcagatacac tgcgaagcag
      421 ctccagaatt ccgtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc
      481 accacaaatg cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc
      541 agcgaatagt tcagcccaaa gagcaagaac cccagccaag tggcgatccc caccacgatc
      601 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
      661 tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct
      721 ttgtctttca gcaggaagaa gaccatcaat ggtaccagca ccagatagac ggcaatggtc
      781 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
      841 cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa tgccggatag
      901 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
      961 atcaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
     1021 ataccgacga aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat
     1081 tgcaggcgca cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga
     1141 gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag
     1201 gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata
     1261 aagaccttcc ctgaacctca agagcgggat tgcgatccgc aattgtatcg aaatgtcaca
     1321 aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa
     1381 tcttcgccgt tacactcaaa ggcggcgcgg tgggaacaat atttcacagt atcggtcaaa
     1441 tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt
     1501 tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcgtttg ccgacagcgc
     1561 agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat
     1621 gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc
     1681 gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt
     1741 taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt
     1801 tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact
     1861 ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat
     1921 ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact
     1981 ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg
     2041 tcaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca
     2101 ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact
     2161 cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ttgactcacc cgttgccgga
     2221 aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc
     2281 gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa
     2341 ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc
     2401 ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa
     2461 aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac
     2521 cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc aatcgcctga aaaatgcccg
     2581 tgatttgcgc accaatccgg tgttgcagct caacctggca aacgcttatc tgcaaggcgg
     2641 tcaaccacaa gaagcggcca atattctgaa tcgctacacc tttaataata aagatgacag
     2701 caacggctgg gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggagct
     2761 ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc gatcaggcca tttcgctgtt
     2821 gagtagcgcc agttcgcagg tgaaattagg cagccagcaa caagcgcgtt acgatgcgcg
     2881 catcgaccag ttgcgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc
     2941 attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag
     3001 agccgggaaa cgctgaacct gctgaaaaaa aacggcgtgg agccagaagt ggtactctac
     3061 cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat
     3121 agcgcccgtg aattgatgcg ccagaaagag gatctttata aagaactgaa cctggctgac
     3181 agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag
     3241 cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg
     3301 gaaatcgtgg gttaagatgt gccccatcgc tagttgaagc acattgccgg atgcgacgct
     3361 tgccatgtct tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc
     3421 gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
     3481 ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
     3541 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
     3601 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
     3661 agtggctgca atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatccc
     3721 agattcaact gccgcggtgg acgatcgccg gtgatcaaca atcgtgtttt gcccgattcc
     3781 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
     3841 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
     3901 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
     3961 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
     4021 taaccgctat gttcctgacg cagcacgttt tgcagcgcag ccagtaaaga ggagttatcc
     4081 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
     4141 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
     4201 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
     4261 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac taatcagttt
     4321 gaagatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt tcagctccgc
     4381 cgcgccaatg tttaccttcg cgccactgac accgatgatc aaaatcacgg aagtcaggat
     4441 caggttctgt gctttgttgt aatccacttt tgattcgatc aaaacacgaa taccggaagc
     4501 accgatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
     4561 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccacc
     4621 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
     4681 ggtatttggc gtagagccaa agaagccgga gatcacagtc gacaggccgt tagcaaacat
     4741 cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
     4801 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
     4861 aaaccactcg aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat
     4921 cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
     4981 taaaatcggg ataattgcca ggaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc
     5041 cagggtggta atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag
     5101 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
     5161 gcccattgcc gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat
     5221 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
     5281 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
     5341 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
     5401 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
     5461 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
     5521 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa
     5581 aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc gtaccaaaga
     5641 ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc agtccctggt
     5701 caatcgatgc ggtatacagt tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt
     5761 ctggcgcagc taccagcacc agaactttga tgctgctgca gcccgctttt ttcagcaggt
     5821 cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc agcgccatac
     5881 gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc gtttcttcat
     5941 tacggtacat accgacaacg ctgatgcgcg cgctcggaac attttccagc acaccgtcca
     6001 tcatccccag accggcacgc agaattggca caacggtaat tttcttacct ttgatctggt
     6061 cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg aggtcggcgg
     6121 tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc
     6181 tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt ttgacttcca
     6241 cgatcttcat actctttctc ctttgagggg cagccacaaa aaaaatcgac ggattatacc
     6301 tcctttcttc aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacggtttg
     6361 agataaagtt attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa
     6421 cctttgccct ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg
     6481 ttgtgcaaag cagtctcgaa aacgtttgct ttccctgtta gaattgcgcc gaattttatt
     6541 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
     6601 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
     6661 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
     6721 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
     6781 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
     6841 catgtgcgtt aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta
     6901 cgcaaccgga aaactggatg ttgataccgc ttcagcggtg atcagcggta tcgcggaagg
     6961 atgtctgcaa tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta
     7021 tcacggtgag gattacgatg tcgcgggttt ctgcgttggc gtggtagaaa aatcagaaat
     7081 catcgacggc tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc
     7141 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
     7201 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
     7261 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
     7321 cggcggcggc ttctgggaaa acattccgcg cgtattgcca gataatactc aggcagtgat
     7381 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg ctggtaacgt
     7441 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc
     7501 tgctccggaa gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa
     7561 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
     7621 gtggtgctta tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
     7681 aacaaaatta aaggcaccat acgggcagtt ttcagcaata aggccgacgc gttcggcctt
     7741 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
     7801 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
     7861 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
     7921 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
     7981 gcgctggaaa atggtgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
     8041 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
     8101 gacatcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
     8161 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
     8221 ccgccgcagg gctacgctgc cgacgagtaa tgccccgtag ttaaagcgcc agctctgccg
     8281 ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc agtttttttc ccccttttct
     8341 ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat
     8401 aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt
     8461 ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg
     8521 aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct
     8581 tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc
     8641 gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc
     8701 tctacaacga gctgctgctg gagatggcgc gtaaccagat cttcctgatt aatgaacgcc
     8761 agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc
     8821 acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg aaagatgatt
     8881 acacctatct ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga
     8941 tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcaccg cgtcgacgca
     9001 agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct
     9061 tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg
     9121 atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc
     9181 gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg
     9241 aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt
     9301 atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca
     9361 aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc aatggttttg
     9421 atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt gagcatgtgc
     9481 tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt
     9541 accgtgtggc aaaagattca cgtatcatcg actcgatgat ccacgctgcg cacaacggca
     9601 agaaagtgac cgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact
     9661 gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa
     9721 ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac
     9781 acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc
     9841 tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc
     9901 cataccgccc ggtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt
     9961 tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca
    10021 ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat gtggcctcca
    10081 gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg
    10141 aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc
    10201 gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga
    10261 tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcgcctga
    10321 agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa gctcgttata
    10381 tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc
    10441 agttggcgat ttatgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata
    10501 cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt
    10561 cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag
    10621 cgggtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg
    10681 cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc
    10741 gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa
    10801 cgcgcggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt
    10861 ctgattttta tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat
    10921 attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa
    10981 agccgccgga tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat
    11041 aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg
    11101 caattccgta ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc
    11161 catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa
    11221 aaactggtaa aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt
    11281 tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat
    11341 gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac
    11401 gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc
    11461 aaccagtatc acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg
    11521 tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg
    11581 cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc
    11641 cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg
    11701 atgatggcga cactggtgcg ctatcaccgt aaagcgatta aactcgacga tctgccgcgc
    11761 tttaccttgt ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta
    11821 ttactcaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat
    11881 gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg
    11941 cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt
    12001 gaagaagaaa gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg
    12061 gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac
    12121 ggctgcggct taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc
    12181 tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc
    12241 agatggcaaa tcgacgccac aatctgataa tccagactat ttgacacaat attgcggata
    12301 aagctgccgt caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg
    12361 tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag
    12421 gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt
    12481 tgccacgctt caatctgata tttagccagc aactgactga cttcaacagg aaaacgagcc
    12541 tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg
    12601 ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa
    12661 ccaaattcgt gcgcgaccgg caagaagcta tcggggacga tcagttcatc attctcacct
    12721 ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc
    12781 atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta
    12841 tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg
    12901 gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc
    12961 actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catcccatcc
    13021 caaaagaaac gaaattgctt gagatgacta tccagtgcgg taatgcgctc ctggtgcgat
    13081 tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt
    13141 tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg
    13201 ccatagttct taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag
    13261 ggagcatcac gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc
    13321 atatacgcaa gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc
    13381 atataattga caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg
    13441 gtatagccag gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg
    13501 accgcccaga gcagcgaaat cagcttataa ccatagcgca tcgctcccca catcatcagg
    13561 ggcagcaata atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc
    13621 atgcataaca gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg
    13681 gtgactttgg catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaatgga
    13741 tttcgcacta cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt
    13801 aaggcctgat aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct
    13861 ctgctcgcca gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca
    13921 ggaaacacaa tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg
    13981 ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata
    14041 aaattagccg ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa
    14101 atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc
    14161 agtgccagag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta
    14221 atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta
    14281 tcacgcattt ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat
    14341 cctgaaggag gtgtattcag acaggcatcc cacctgactt cgaatgatga ttattcatca
    14401 ctatagagag cattgattct aagtgtcata tgaaagttcc aattgatata tatcaaacaa
    14461 aataaccctg attaatgaat tattacgttt atcatgttaa ttcatcatta ttacatcatc
    14521 attgtaaata attaaattaa cttccataac attaaaatat gtatccactg acgttttttt
    14581 acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac cgtccacagc
    14641 gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga aaacgacacc
    14701 gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc ttctttggcc
    14761 gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa gaagctcagc
    14821 aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct tcactttcac
    14881 agaacataac ggcacttcgc tgtcggatgc ttttgctctt tgggattatc aaagcggcag
    14941 atattctttc atcttaaatt ttacgtcttt gtcctgactg atgtttatcc tgtttggctg
    15001 cggaataaat ataaaattaa tatatatgtt gtaatgatat atttttataa attattccct
    15061 gcgtgaattt taataaattt aatctatccc tttatacgca atacatttac tttcctcttt
    15121 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
    15181 aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg atgccagtca
    15241 aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
    15301 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
    15361 aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc
    15421 ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga
    15481 gctgtaggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
    15541 ttaacatata aggaaggcac caaagtgtat acctccaccc aggtgggtaa agagtgccag
    15601 tttacgacag gtttagccgt tgttattacc acaacgtata acgaaacgcg tattcagcca
    15661 aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt
    15721 tgcgccatct tagcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
    15781 tctgctgtcg cagaagcgga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa
    15841 tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag
    15901 gccgccgcta acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa
    15961 ctggacgccg aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
    16021 agcttagaga ttcagaaaca aaaactgacg ctggcgaaag aagaagcccg cgttaagcga
    16081 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
    16141 tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg
    16201 ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca
    16261 agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat
    16321 agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat
    16381 acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga
    16441 aatcgtacgg cagacgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca
    16501 gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca
    16561 ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa
    16621 tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca
    16681 cacgaacgcc aaggcctggt cccgggaatg ggtgacggta caacatgtcg tacggcaggc
    16741 ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca
    16801 ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg
    16861 ctttaccggt tgcagacgcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca
    16921 gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgac
    16981 cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat
    17041 cttctgccgg aacgtgaaca atgttcagac caaagtgatc gccaaacata tccagaacct
    17101 gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac
    17161 cgatagcgcg gtgcagtagc attgcggtta cggaggaatc cacgccacca gagaggccga
    17221 ggatgacttt atcgtcgcct acctgctcac ggatgcgggc tacagcatcg tcgataattt
    17281 tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc
    17341 gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt
    17401 cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg
    17461 acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt
    17521 ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcact gtcgtttacg acttcaacct
    17581 gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca
    17641 tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg
    17701 gcgcacgcgg gctgttttct tcagtagtac tttccgggcc gccggaaaga ataatgccgc
    17761 ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa
    17821 cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtctagaa
    17881 tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa
    17941 cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta
    18001 gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc
    18061 gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag
    18121 acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt
    18181 ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg
    18241 gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga
    18301 tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag
    18361 catggaacct accatcaccg cgcttgcgcc agcggcgata gctttggcga tgtcgccgga
    18421 gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc
    18481 gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat
    18541 agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc
    18601 acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt
    18661 ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga tcagcagaac
    18721 gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac
    18781 tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc
    18841 tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag
    18901 cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc
    18961 acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc
    19021 ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac
    19081 gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt
    19141 ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacggcg
    19201 aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc
    19261 ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat
    19321 agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag
    19381 aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat
    19441 acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca
    19501 gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggcg
    19561 ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt
    19621 ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg
    19681 agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt
    19741 ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc
    19801 gcaacagcaa ccgccgggtg accttccgtc cacagcatgg acaacaagtt ttagttcgcg
    19861 ccaatattac gctctacgag ccgcgcggtg actaccagat aatcgttgag agtatgcagc
    19921 cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg
    19981 aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg
    20041 tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg
    20101 atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac gcaccggggc
    20161 aaattgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg
    20221 ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg
    20281 cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca
    20341 ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga
    20401 gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg
    20461 cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac
    20521 agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa
    20581 agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt
    20641 taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca
    20701 ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg
    20761 aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc
    20821 gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta
    20881 aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagttaaaa
    20941 acatccagcc agtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc
    21001 gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt
    21061 ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc
    21121 cgcaggatgg gcaacgcgca tggccgttat cctgatcaag cacatgctga cattgtggac
    21181 aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag agggaatggc
    21241 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
    21301 tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc
    21361 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
    21421 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
    21481 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag
    21541 cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac
    21601 acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac caacaccact
    21661 gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc
    21721 cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac
    21781 aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
    21841 aataccttcg cgcgcatcaa tcactaacat caccacgttg gtatcttcaa ttgcctgcaa
    21901 cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
    21961 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc
    22021 acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
    22081 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
    22141 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
    22201 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
    22261 cacacgctcc agcagactta atacgccacg accgtgagat gcggcgatcg ggtagatttc
    22321 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
    22381 gtttgccacc aggaaggtcg gtttttcacg agagcgcaga tgtttggcaa tcgcttcatc
    22441 tgccggcatc aagcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
    22501 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
    22561 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
    22621 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
    22681 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
    22741 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
    22801 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
    22861 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
    22921 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
    22981 gttaatccag tgcagataac cttcactgtc acctaccacc aggttgccat tatacagcac
    23041 cggcgaagtc agcaggcgat gcagcagatc gctttgtgtc cacagcgtaa cgccgccatc
    23101 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
    23161 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
    23221 aagcgccgtc aggttaccat tataggccag agcgaaaaca acgccgttaa caacgacggg
    23281 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
    23341 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
    23401 ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
    23461 aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttca gcgcttgtaa
    23521 ctgaccgtta ctagtgtgga ttaacaccag accgtcgctg accaccgggc gagaaagtgc
    23581 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
    23641 ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcacac cgccagaaag
    23701 taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag accagatttc
    23761 tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac
    23821 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
    23881 agtgctccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt
    23941 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
    24001 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
    24061 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
    24121 atgcactacg cgcaccttgc ttatcacctt tgctcaacaa tgctttacca cgcaggtcgg
    24181 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
    24241 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
    24301 catcactcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
    24361 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
    24421 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
    24481 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
    24541 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
    24601 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
    24661 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg
    24721 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
    24781 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
    24841 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
    24901 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
    24961 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
    25021 acgttcgagg cccatcgcaa aaccgactgc cggtgttgca cgaccgccca gttgttccac
    25081 cagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgaggc tgttagtcac
    25141 ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt
    25201 gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc
    25261 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    25321 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    25381 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    25441 cagcgaacca atagagttca gctcaagagt tacgtgttcg aagataccca gcgcacgcca
    25501 ccagcgggca gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    25561 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    25621 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    25681 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    25741 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    25801 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    25861 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    25921 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    25981 gaaccctcaa cgcttccctc aatgtttcgg gagccatgcg ggttcaatca tacacgggaa
    26041 gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct
    26101 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    26161 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg
    26221 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    26281 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    26341 ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttaat ccctcgcgaa
    26401 cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    26461 gccagcgata cgcgcagcgt gtcgccgatg ccttccgaca gcagcagacc taaaccaatg
    26521 gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc
    26581 tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    26641 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atgacgcatg
    26701 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    26761 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    26821 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    26881 ccgtattccg ctactttcag cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    26941 acgttaaccc gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    27001 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    27061 acatctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    27121 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    27181 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg
    27241 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    27301 actggatctg tactgcggct ggcgcaccaa ttttcagttt atacggtgcc tggccggtta
    27361 aattcaaatt accgtcttta cgctgcatcc cgctaaacaa ttttttaccg gtagcatcag
    27421 tgacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    27481 gcgtggtcac gccagcctga tcggttggca acggcgcagc accatctggc gttgttgctg
    27541 ccgttggtac cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat
    27601 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    27661 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg
    27721 tatttaacgg gacactctgc ccctgctcgc tattactgct cagttctgcc gaagattgat
    27781 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    27841 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccatcacgtt
    27901 ttttgcggcg tttaccgagg gaaaaactct gcattggcgc aacttttgca gcccgtagtg
    27961 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    28021 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    28081 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    28141 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    28201 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    28261 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    28321 ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc
    28381 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    28441 caacttaata cttcattcat aaaacattac gccacggtac ataaagtaac cgtggcgtaa
    28501 tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    28561 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc
    28621 acgagtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    28681 gatacggctg ttcgagctgc gtccatacgg cgcacccggg aacgggttcc acgggatcag
    28741 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc
    28801 gtcgttaacg tggtcaagca tcacgtattc aatagtgact cggccctgat tggcgttgga
    28861 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    28921 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac
    28981 gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac
    29041 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    29101 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattggccg
    29161 ctgaccggtg acttttgctg cgccgacgat tttcgccgcc cgccacacct ggccgataat
    29221 ttccgacacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    29281 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    29341 caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga
    29401 acgctgttct tcaaccactt ccggtgcgcg gatctccgcc acctctttca gtttgccgcg
    29461 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    29521 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    29581 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    29641 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    29701 gagggttgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    29761 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    29821 cgcgggcaca cttcgccttc gccgaagaaa taagcgattt cgcgagcggc agattcgacg
    29881 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    29941 ccggccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    30001 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    30061 tcaaagaacg gttttccatc gtgttcagca taaaagccac gagcctgttc aacggtcagg
    30121 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    30181 ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc
    30241 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    30301 tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    30361 agttttagca acaatcaggg acaaaacaga ctattgcatg acaaatttca ctgtcgcgat
    30421 ttgccccacg tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt
    30481 cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc
    30541 ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat
    30601 cgcgccatcg cgaacgcctg tcagctgcag agggagctgg gcatcgtgac cgtatggcgg
    30661 gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg
    30721 ttccagcggt agcggccaga catttaccat ttcttgcttc gcctgcgggc agtcagcggc
    30781 gacgcgctgc cctttgtcat ccagccagat ggggaagcga atgccattaa tcccttcctg
    30841 ctctgacagt aacagcgttg gcggctggct gccatccagc agccaggttg ccaggcggcg
    30901 gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg
    30961 agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt
    31021 attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt
    31081 gccgtcaggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca
    31141 ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa
    31201 ggcactatcc ggcaagggtt gcgcttcatc agccataatc cggcgaatga tccacgccgc
    31261 ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc
    31321 tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc
    31381 agcgccgccg agaatgagtg aaagattcgg cgcagcgccg ttgggcaaat ataacggcaa
    31441 tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac
    31501 agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc
    31561 gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga
    31621 tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga
    31681 tcctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag
    31741 atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc
    31801 cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg
    31861 ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat
    31921 gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc
    31981 ccttgactct tttacctgct cacgggacca cacaccttgc gcagccatcc gttcgagcac
    32041 tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc
    32101 ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc
    32161 gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt
    32221 aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca
    32281 gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg
    32341 catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc
    32401 gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat cttcataatt
    32461 gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat
    32521 gccgtcagca tcggcgaagc gccagagcgg cgtaccatcc tgcgccacga ccactcgtgc
    32581 gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa
    32641 aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta acaagcgagg
    32701 catttacggt cggacaatca gcagatcttc agccgcgccg gtcgcccgcc attggggaac
    32761 atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc
    32821 cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc
    32881 gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttctgcac
    32941 ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctggt tttccagttc
    33001 cagacccaca ggcagcagat ccacgactaa cgcatccggc acgctgttac tggcttttac
    33061 ctgcaaccac accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc
    33121 atcagtacca agaatatgac gctcgatttg cagcacattg ttcgccggta aaggtgcgga
    33181 ttgcggataa ccgctggcat ccatacgcaa ccataacggc tgatcgccac tgttgctcac
    33241 ctgcaaggtg acaagttgat cgctattcag attgctgttt tgcgctttct cgcctgtcag
    33301 cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg
    33361 ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaacgcctg
    33421 ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt agcagtttat tttcttccag
    33481 caaggagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg
    33541 ctcatcacta tttctcggcg ttttcagcgc cagcgcaatc gcttcttcac cacgcgtcgc
    33601 atcacccatg gttttcagcg caatgccaag ttgcagcagc ggtaaaccag aagcggcatc
    33661 tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct gacgggccag
    33721 caccagcgca gcgtaagact gaacggcgaa tttactggct ttgagattat ccgcgtacgg
    33781 gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat
    33841 ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta
    33901 agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc
    33961 acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc
    34021 actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa
    34081 caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata
    34141 acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg
    34201 cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt
    34261 accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttatg
    34321 ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct gaatttctcc
    34381 atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc
    34441 taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg cggtcagggc
    34501 aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt
    34561 atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat
    34621 cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa
    34681 ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt
    34741 taccgacagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg
    34801 tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat
    34861 aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg
    34921 ccacggatct ggcgtaacgt agtcagtaat attcagcaca ccactatcaa cggcagacac
    34981 cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt
    35041 taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt
    35101 ttcatcacca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga
    35161 tttatcgcca ggacgcacca ccagcgtact caaataaaga tcatgacgat tccaggtttt
    35221 atcgaccgga atcgtcagat ccagcccttg agccggaaca tcaatctctt gccaccacag
    35281 cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg cggcaatatg
    35341 caacttaatg gtatcgccgg ggcgataact ggctttatct agtttcagcg tgacacggtc
    35401 gggtcgcact gcgccgctcc cgtcgctgtt gtcctgccag ctatagccag cccaaaaacg
    35461 cacgctactg accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac
    35521 cgggaaactg accttaccgg tttcatccgc ctgaagatcc agagtttgtt cattttcgat
    35581 cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta
    35641 atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg
    35701 cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa tgggctgttt
    35761 caccgtgctg tcagtgcgat aatcgtaaac cgatttcaag gcgaactgcg gacggagccc
    35821 cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc
    35881 cgattccagc aaactgccct ggaaaataac ctgcaatggg gaatgcgttt ccttccactg
    35941 gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag
    36001 cgtgcgggaa aggttttcgg cagctatatc gccgaattca aaaccaggta aggctgacac
    36061 agcctcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc
    36121 accgtacagg tagtatccca ccacggagaa tttcacttca tcattcggcg taagcggggt
    36181 tttctcaccg gtcagattca gcgccatacg ctctggcata aaatcttcga cgtggaaatc
    36241 ccacatccgg tactggttat cgcccgtgtt ggcgcgaata tgccacatac cggttgccgc
    36301 attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac taacgacgtt
    36361 cctgagtacc tgcccgtcag gtttaatcac gtctaacttg atgggttgat cgggcaatgc
    36421 tttaccgtct gcatcgcgca gtaaaccatt gaggattacc gtttcacctg ggcgataaag
    36481 atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa
    36541 ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg
    36601 tgccagcagt aatgccgcat ttttatcatt ttccagctgc acatgcccct gagcgtcact
    36661 ggttgcctga gtcagagtct gtcctttctc atttaataaa gagacttcaa ttccttgctg
    36721 ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat aacggtgagc
    36781 tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc
    36841 agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag
    36901 cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta
    36961 gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta
    37021 ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc
    37081 gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag
    37141 cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc
    37201 gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat
    37261 caaatcacgt ttcggttcga ggtggcgtaa acgtagctct ttaagattat ctgacagctc
    37321 ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc
    37381 ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg
    37441 gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg
    37501 tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac
    37561 ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc
    37621 cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca
    37681 caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc
    37741 agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag
    37801 cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa
    37861 tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg
    37921 atgacccgga aattcagatt atcgatgccc gcatggcgtc gcctggacag gaagatcgta
    37981 acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag
    38041 cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg
    38101 tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat gacgaaggta
    38161 atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt
    38221 cgattctggg gggtggactt gcaggctggc agcgcgatga tctgctgtta gaagaaggtg
    38281 cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag
    38341 taaccgatgt attattggca agccatgaaa atacggcgca aattattgat gcccgcccgg
    38401 ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc
    38461 ccggagcact gaatgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg
    38521 atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca
    38581 gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc
    38641 caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg
    38701 ttgagccagt gaaataagta ttttacaggc aataaaaaac cgccgaattt ggcggttttt
    38761 tattgctggt ctggttcgcg gcctttccag caggttgtat taccgtagta atgcaagcgc
    38821 gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa gagagttatt
    38881 attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg
    38941 gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc ttgctatcgc
    39001 ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat
    39061 acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag taaataataa
    39121 ccggagtctc tccggcgaca atttactggt ggttaacaac cttcagagca gcaagtaagc
    39181 ccgaatgccg ccctttaggg cggcatattt tagattatcc ggttttgttt aaagtcacgc
    39241 aaaaaaccac cccagcgacg ttcatagaat ggcacaatat gttcggtaat aaagtggcta
    39301 attccttttt cccctttttt cacctgacaa atatcgattg gttcatcgcc aggtaatgta
    39361 tcggtcgcta cacttcccgt cgcctgaata atttcttcga tatcaccatc ggcttcaatg
    39421 ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc
    39481 ttcaccggct taatggtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc
    39541 tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg gctgctcagc
    39601 ggatttccct cttcaccaat caacaaactg atttcacgcg gcataaattc tttaccggtc
    39661 ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg
    39721 ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc taacgaggtg
    39781 aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc aagcgcacta
    39841 tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt ggattccagt
    39901 agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct
    39961 tcaagttcgt tttttgtttc ggacataaga accacaattc attcaacgtt cggggcgcaa
    40021 atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag
    40081 aatttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac
    40141 agattagcta tcgtgcgcac accaagtccc gtagcgcccg cagaccactg ttcaaccggc
    40201 gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca
    40261 aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta
    40321 ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga
    40381 cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg
    40441 tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg
    40501 gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca
    40561 agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag
    40621 gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg
    40681 aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc
    40741 gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg
    40801 tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct
    40861 ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt
    40921 ccgacggtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga
    40981 taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc
    41041 gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc
    41101 aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc
    41161 gtgcctttcg gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct
    41221 tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg
    41281 atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg
    41341 taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc
    41401 gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat
    41461 ttttccgcta aatgattcgc gttgcagaaa ggcggcaagt gagtgaagcc ccaggagcat
    41521 agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga
    41581 cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag gattttctcg
    41641 ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg
    41701 gtgaatcgaa ccgttttcgg atcaagatca ggatacgcat cgtacagtgc ttcgccaatt
    41761 tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
    41821 tgatagtgta acgcgggatt tcgactacta aatcttcgtc ggtaacacgt gcctggcagc
    41881 ttaaacggct ttcgggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg
    41941 agctttctgg cagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac
    42001 aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg tcgagaatgg
    42061 tttcaccgct attagcttcc agaacagcgc catcaggaca gagatcctga tgaggcaaaa
    42121 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
    42181 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
    42241 tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg
    42301 tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc
    42361 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg
    42421 tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga
    42481 ccgtaagacg gtttgacctg aatagacgcc tcaacgctgg tggatttctc catcgccgtc
    42541 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accaccagcc
    42601 ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt
    42661 tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg
    42721 aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg
    42781 ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg
    42841 tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
    42901 tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc
    42961 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc agcttctaca
    43021 cccgcgtctt taagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc
    43081 agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg
    43141 gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga
    43201 acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga
    43261 tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg
    43321 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacagcg
    43381 atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta
    43441 agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
    43501 gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct
    43561 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc
    43621 agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc
    43681 agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa
    43741 ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc
    43801 ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac
    43861 gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata
    43921 ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
    43981 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
    44041 actgctccgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca
    44101 cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
    44161 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc
    44221 ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
    44281 ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag
    44341 cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg
    44401 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
    44461 cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagaaggtga agtaatccat
    44521 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg
    44581 cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
    44641 ccacactcat ctttgacgtt cgggttggtg aatttaaacc cttcgttcag gccttctttt
    44701 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
    44761 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
    44821 taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg
    44881 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata
    44941 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
    45001 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
    45061 agcaatatcg gtatttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc
    45121 ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc
    45181 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc cacaggccgg
    45241 tgcccccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg
    45301 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
    45361 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
    45421 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
    45481 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
    45541 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
    45601 acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc
    45661 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
    45721 gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg
    45781 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcctcgccca ttccgacgat
    45841 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg
    45901 cgcttcgatg cgtacgcgcg gtttacgacg tacatacagc gcaccgatac ctttcgggcc
    45961 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg
    46021 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
    46081 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga
    46141 aacaaggatg gtgtcatcac gcatcgctgc ttcaagttct ttcagatcga taatgccgtt
    46201 acgctgcggt gccaggtagg tgacttcaaa accttcgcgc tccagctgac ggcaggtatc
    46261 cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa
    46321 gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat
    46381 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
    46441 ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg ttccgtccat
    46501 cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
    46561 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
    46621 accaggctac cggctggatg tacgaccgtg tttacggagc gcgatacgcc ccggcctgat
    46681 tctaatatct tgttatcttt aacctaactg tttcgggttt cagcaagttg acaaacttac
    46741 gaatctccgg gagcttacat aagtaagtga ccggagtgag tatgtgcagt caacgcagat
    46801 gcaacacgaa acaggacggt taaattaagc gcgtaactta acgtcgatcg cgtcttgtat
    46861 gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt
    46921 attaaccagt tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc
    46981 acgccacagc gcgtgggtca ggcatttatc gccgccctgg caaccgcctt taccctgaca
    47041 acgggtagca tctacagatt cgtcaacggc gctaatcact tcgccaacgg cgatgctgct
    47101 ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt
    47161 tttacgcaga cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga
    47221 aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt
    47281 cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct
    47341 gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc
    47401 ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc
    47461 cattttttta tcgtggcgtt tgtacctgtt gtccttccag accatgggcg ggttgagggc
    47521 aattattcgc attagcgctg gcctcttccc ctcaccctaa ccctctcccc gaaggggcga
    47581 ggggaccgtc cgtgcattta tctggcttag ccctcaggcc ttattacctt tattctgctg
    47641 ctctatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgcgcacg
    47701 ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac ggataaaacc
    47761 ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat cgaccagcgg
    47821 atacggcgtc tcttcatgct cgacctgctc gccattttcc tgagttgcca gccaggccat
    47881 acgcacttca taggcaataa cctgaaccgc cattgccaag ttcagcgaac tgtattccgg
    47941 gtttgccgca atcgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac
    48001 gcgctcacga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag
    48061 accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggagcgtg cgctggtgcc
    48121 caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc
    48181 aatcacatcg ctggcccctg ctgccagggc aatcgcctgg gagtcgggtt tcaccagtgg
    48241 attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaacccat
    48301 attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct
    48361 tcatctaaag attattcacg catcttatca taaaacgaag acagatgccg atctcgctgc
    48421 tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat
    48481 gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga
    48541 aaccccggac gctgtagaag cgagccagaa aggcagtaac gatttcgtga ccaacgtaga
    48601 taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttactcac agcacaccat
    48661 catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga
    48721 tccactggat ggcactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc
    48781 cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc gtaacgaact
    48841 gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggtagcac
    48901 cgctcgcgat ctcgacggta ccattctggc gaccggcttc ccgttcaaag caaaacagta
    48961 cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg
    49021 taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg acggtttctt
    49081 tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag ctgctggttc gtgaagcagg
    49141 cggcatcgtc agcgacttca ccggtggtca taactacatg ctgaccggta acatcgttgc
    49201 tggtaacccg cgcgttgtga aagccatgct ggcgaacatg cgtgacgagt taagcgacgc
    49261 tctgaagcgt taatgactca ggcgggtgat gtcactcacc cgccctcgcc tttcaggcgc
    49321 tattctgaaa tacttcctca ccgctttact ttctttcgtc actctcccac catctttccc
    49381 cgattaatgg atgataaaga agtcacaagg aaaggttatg gcgctgccag tgaacaaacg
    49441 cgttctcaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg
    49501 cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg acccctggtc
    49561 ggcggaatcc gtcgaattta cggctaagga cggtactcgt ctgcaaggct ggtttattcc
    49621 ttcttcgacg ggccctgctg acaatgccat cgcaaccatc attcatgctc acggcaatgc
    49681 cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt
    49741 ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc aggccggatt
    49801 gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca
    49861 acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg ttattggtca
    49921 ggatgatcgt gaaggcatac gtgcggtgat cctcgactcc acatttgcct cttatgcaac
    49981 catcgccaat cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga
    50041 aaattatatc gccagcgtca gcccgatccc gcttttactc attcacggta aagctgatca
    50101 cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagaga caaaacggtt
    50161 gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgtcacg gcgatgttta
    50221 tcgcgaacag atggtggact ttatccttag cgcgttgaat ccgcagaact aacccatgat
    50281 cgctagcacg ataatcattc acaaaaccac cttaagacat gctaatccac tggtcagaac
    50341 agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt agcattccct
    50401 tcgccatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac
    50461 agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga
    50521 tcttgctgac gctctttcag ccggggttga ccgccaccat ggcaaccttc agcgagctta
    50581 atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg gagatcctgc
    50641 gctatcatca actcacactg acgactggtt atgacggtag ctaccgggtt gaaggtacag
    50701 tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt ctgtgtccgt
    50761 catttattac cagccaattc acccccgccc tgaagagtga actgaagcgg cgcggaattg
    50821 cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc tcccgacggc
    50881 tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg tctgtatctg caatattgtt
    50941 tgctgcaaca ccacgctgga attacgcctc agtttaatcc gctccaacgt cgctgggcgg
    51001 aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgggcgc
    51061 tccagcctgt accgcctgat gagccactgt ttatggcatt acttttttcc atgttgcggg
    51121 ttcccgatcc attgcgtgat gcgcatcagc gagacagaca attgcgtcag tctatcaaac
    51181 gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa caggggttat
    51241 gcgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta
    51301 ttgataatac cctgccggaa gagttcgcca gactgtaccc acgcctggtg cgcaccaccc
    51361 gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagcg
    51421 gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg catgaaaaac
    51481 agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag cagcagttac
    51541 gcgaactaac gttactgccg ctcaacatta agcatatgtc ggtaaaggca tttttgcaga
    51601 caggcgctcc gcgtggcgcg gcactgatta ttgcgcctta taccatgcca ttaccgctct
    51661 tttcaccacc gctgatctat acggacctga cgctgacaac acatcaacag gagcagatcc
    51721 gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggaagc
    51781 gccaccagcg ccattaccca gaacacgccg tggcccagat attgatacag gaaaccggcg
    51841 aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa
    51901 cggatgactt cgctaccctg gcgaacagca atatagcgca tggcggccag gtggcagacc
    51961 gtgaaggtgc cgcaatgcaa aatttgcacc actatcaacc acggcaacgc cgtagttgct
    52021 cccataatgc cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgagca
    52081 ctacaacggc ggaaaagttt attactcagc gcaaagataa tgacttccgc caccacgccc
    52141 agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg
    52201 ctaaaaccgt aataggccgc atgtgccccc tgcaataaac aaacgcaggc cagaaagcgc
    52261 cagttctggc gaaccagcgc caaccacgca gaccaaccgg tgctctcctg ctggcgactt
    52321 gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc
    52381 aacgtcaaca gcgcgaggat cacccgataa tcaaacatag tgaccagttt gcccgtcagc
    52441 gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta atcaagcggg
    52501 aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag
    52561 aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg
    52621 acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgggaagga
    52681 tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag acctgccccc
    52741 aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac gctccagaaa
    52801 ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg
    52861 gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggggtta atcgaatgtt
    52921 ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta acgccagttc
    52981 acaagcacta aaaaactgcg atccgcgacg catgttgcaa aaataatgcg taattatgcc
    53041 gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc cggcttcatc
    53101 tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc aatgggacga
    53161 aaaacaacct gcccggtatt aaaattattc atataaccgg gtatcaaagt gacgcccagc
    53221 cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac gatatttggc
    53281 tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg caagcgaacc ggaatacgcc
    53341 ggatcggtac tgacgaaatt cacgccatcc agttacgcgg cggtgatctc tttttcatgt
    53401 gctaaagggt gatcaaccgg taacaccacc actaatggtt cgtcaaaaag ctccagataa
    53461 tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc accacggcga
    53521 attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt gtctggctgt
    53581 ctgagacgaa acattggtaa tacttttggc aataaattca cttccgccga tggcacaaag
    53641 ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt ggcattttcc
    53701 gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc cgtcaacgcg
    53761 actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag atcgcggatc
    53821 tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt aaagttcagt
    53881 gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt ccccttgtta
    53941 tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa ccaaccaaca
    54001 acatccttat gacacaaaaa tagaaggtca atacatctta tctttcagga ttaaaaaata
    54061 tgaccacacc ctcagattta aacatttacc aactgattga tacccaaaat ggtcgggtca
    54121 ctccgcgtat ttataccgac ccagatattt accaactgga gcttgaacgt attttcggcc
    54181 gttgctggtt atttctcgcc cacgaaagcc agatcccaaa acctggtgat ttctttaaca
    54241 cctacatggg ggaagatgcg gttgtcgtag tgcgtcagaa agacggcagc attaaggcgt
    54301 ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc ggcaacactc
    54361 gcgcctttac ctgtccgtat cacggctggt cttatggcat taacggcgag ttgatcgatg
    54421 taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg ggactaaacg
    54481 aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat accagcgcac
    54541 cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg ctggatcgtc
    54601 gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac tgtaactgga
    54661 aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc catgcttctg
    54721 ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt gatggacaaa
    54781 ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca atttggtcag gacggtcacg
    54841 gtagcggttt cttctttact gaaaaaccgg atgctaatgt ctgggtcgat ggcgcagttt
    54901 caagctatta ccgcgaaacc tatgccgaag cagaacaacg tttaggtgaa gttcgcgccc
    54961 tgcgcctggc gggtcataac aatattttcc ccacgctttc atggctcaac ggcactgcca
    55021 cgctccgcgt ctggcatccg cgcggccctg atcaagttga agtgtgggcg ttctgtatta
    55081 ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc actcgtgctt
    55141 ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa atccagaaat
    55201 tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg cttggtcagg
    55261 aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca gaaactgccg
    55321 ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg caggaagtgc
    55381 tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag tttcactaga
    55441 gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg acgactggaa
    55501 atttcgtgac tggctggagc agctcgacaa agagattcgt tacaccatgc gcaccacagt
    55561 taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca ccgacaacct ggatttttaa
    55621 tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca tggcctgggc
    55681 agaagagccg ccgtcacgca cccgtcactt aatcagcaac tgccagataa gcgaaaccga
    55741 catcccaaac gtatttgctg tgcgggtaaa ttatctgctt tatcgggcac aaaaagagcg
    55801 cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt cgccgtctgg aagatgacaa
    55861 ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt cccataacct
    55921 gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt gccggagggt
    55981 gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt tcaacgttgg cggcgagttt
    56041 tatgccatta acgatcgttg cagccatggt aatgcgtcga tgtcagaagg gtatctggaa
    56101 gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa aacggggaaa
    56161 gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt tgaaggtggt
    56221 gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac gagtccattt
    56281 ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt atcgaagaag
    56341 gcgcgcaagt tgccacgctg gaactgtcgg cggcaaaagt cgccagtctg cgtcagcgat
    56401 ttggcgaaca tattctggcg gtggaaggta acgtgacctg ttatgccgat tatcaacgcg
    56461 cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc aatgcaggca
    56521 tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa accggcttcc
    56581 acgagctgtt taacgtcaac gtactcggtt acctgctggg cgcaaaagcc tgcgctccgg
    56641 cgttaatcgc cagtgaaggc agcatgattt tcacactgtc aaatgccgcc aggtatcctg
    56701 gcggcggtgg cccgctgtac accgccagta aacatgccgc aaccggactt attcgccaac
    56761 tggcttatga actggcaccg aaagtgcggg tgaatggcgt cggcccgtgt ggtatggcca
    56821 gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac ctcgataatg cagtctctga
    56881 cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg gcggatttta
    56941 cggggccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc ggtgtgatga
    57001 tcaacgctga tgcgggttta gcgattcgcg gcattcgcca cgtagcggct gggctggatc
    57061 tttaaggaag cacgatgaaa gaaaaaacga tcattattgt cggtggcggg caagcggcgg
    57121 caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat ctgttttccg
    57181 atgagcgaca tcttccttat gaacgacctc cactctcgaa atccatgttg ctggaagatt
    57241 ccccgcagtt acagcaggtg ttacccgcta actggtggca ggaaaacaat gttcatctgc
    57301 attccggtgt aaccatcaaa acgctgggtc gcgacacacg agagttagtg ttaaccaacg
    57361 gcgaaagctg gcactgggat cagcttttta tagcaatcgg cgcggcagct cgaccgctgc
    57421 cgttgcttga tgcactggga gaacgctgct ttaccctacg ccatgccggt gatgccgcca
    57481 gactgcgaga agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc ggaactattg
    57541 gtctggaact ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg attgaactgg
    57601 cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt ttacagcgcc
    57661 accagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg gtcgatggtg
    57721 aaaaagtaga actgacgctg caaagtgggg aaacgcttca ggctgatgtg gtgatttacg
    57781 gtattggtat cagcgccaac gagcaactgg ctcgcgaggc caaccttgat actgccaatg
    57841 gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc ggtggcgatg
    57901 tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg gaaaacgcca
    57961 ataaccaggc gcaaattgcc gctgccgcaa tgttagggct accgctaccg ctactgccgc
    58021 cgccgtggtt ctggagcgat cagtacagtg ataacttaca gtttattggc gatatgcgtg
    58081 gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa ggcgatttgg tttaatctgc
    58141 aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg gcgtgagatt cgcccaattc
    58201 gcaaatggat ccagagcggc aaaacgtttg atgcgaaact gctgatagat gagaacatcg
    58261 cgcttaaatc actgtaacca ggataattag cgaatatctc aacgcctggg gcgtggcgag
    58321 gtgcaagagt gtgtattacg tttaaatcac attatcttgc aaagggattg gttatgaaca
    58381 cacta
//
LOCUS       DAJTYZ010000009        57841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000009 DAJTYZ010000000
VERSION     DAJTYZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.009"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            175..1293
                     /gene="hyaA"
                     /locus_tag="OO596_000521"
     CDS             175..1293
                     /gene="hyaA"
                     /locus_tag="OO596_000521"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6716474.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            1290..3083
                     /gene="hyaB"
                     /locus_tag="OO596_000522"
     CDS             1290..3083
                     /gene="hyaB"
                     /locus_tag="OO596_000522"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6716475.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            3102..3808
                     /gene="hyaC"
                     /locus_tag="OO596_000523"
                     /pseudo
     CDS             3102..3808
                     /gene="hyaC"
                     /locus_tag="OO596_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            3805..4392
                     /locus_tag="OO596_000524"
     CDS             3805..4392
                     /locus_tag="OO596_000524"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6716476.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            4389..4727
                     /gene="hyaE"
                     /locus_tag="OO596_000525"
     CDS             4389..4727
                     /gene="hyaE"
                     /locus_tag="OO596_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6716477.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            4784..5455
                     /locus_tag="OO596_000526"
                     /pseudo
     CDS             4784..5455
                     /locus_tag="OO596_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
     gene            complement(5813..6424)
                     /locus_tag="OO596_000527"
     CDS             complement(5813..6424)
                     /locus_tag="OO596_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6716478.1"
                     /translation="MDKAEKTVSSAERFIDMEGINRFSERLAMAMGNMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLDWLATGKNEDECGKIGKNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
     gene            6581..6847
                     /locus_tag="OO596_000528"
     CDS             6581..6847
                     /locus_tag="OO596_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6716479.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            6858..8948
                     /locus_tag="OO596_000529"
     CDS             6858..8948
                     /locus_tag="OO596_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6716480.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSSAVVDAVEAYRGSPQ
                     LMLERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEIVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKESDPLA"
     gene            9031..9963
                     /locus_tag="OO596_000530"
     CDS             9031..9963
                     /locus_tag="OO596_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6716481.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLTGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            9966..10187
                     /locus_tag="OO596_000531"
     CDS             9966..10187
                     /locus_tag="OO596_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716482.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKACGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            10200..10454
                     /locus_tag="OO596_000532"
     CDS             10200..10454
                     /locus_tag="OO596_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716483.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            10456..10737
                     /locus_tag="OO596_000533"
     CDS             10456..10737
                     /locus_tag="OO596_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HCR6716484.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIDVVA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMVQFCREVRRRVSYPNRTRARG"
     gene            10734..11006
                     /locus_tag="OO596_000534"
     CDS             10734..11006
                     /locus_tag="OO596_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716485.1"
                     /translation="MSKVVRIIFEYKEDVIRKNADGTVHMGVSLDIRSTGIKQKNDSP
                     AMIFGVVMLAESRNFAELVAMKASAFMKDRGMASGVINGNEFNQQG"
     gene            11011..11304
                     /locus_tag="OO596_000535"
     CDS             11011..11304
                     /locus_tag="OO596_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716486.1"
                     /translation="MSKVRVIFEFEHVSHDEKPAGNDSVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            11316..11846
                     /locus_tag="OO596_000536"
     CDS             11316..11846
                     /locus_tag="OO596_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HCR6716487.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRRFGDMQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIHDWCKTNRNELTNGGETKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKQAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            11944..12486
                     /locus_tag="OO596_000537"
     CDS             11944..12486
                     /locus_tag="OO596_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716488.1"
                     /translation="MAYFYFKLDIVYTNKYLTEYQRDVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGSLPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIET
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNTDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            12490..13023
                     /locus_tag="OO596_000538"
     CDS             12490..13023
                     /locus_tag="OO596_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716489.1"
                     /translation="MIDAKVLEGVKSWLRFSGRLTSRSLAEKMNMPLSSMVYFLRDAV
                     DAGVLTDRNGFYDIPRPRPVQAVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITGRVLRY
                     LIIDRTAASAGISAEVA"
     gene            13023..13538
                     /locus_tag="OO596_000539"
     CDS             13023..13538
                     /locus_tag="OO596_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716490.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFTEDKYPFHRDLWRKPTASNGWSCTPRTSRIPKTLRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVKWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            13542..14093
                     /locus_tag="OO596_000540"
     CDS             13542..14093
                     /locus_tag="OO596_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb domain-containing protein"
                     /protein_id="HCR6716491.1"
                     /translation="MMKLQPMGLKGRAPAHVRPWTPEEDALLIALYSSTPVKDIAARI
                     KRTVWAVYNRTGVLRSSYPELLKYKHPRFTPDEDKFIRKNARTMTCQQMGEYLGRNKD
                     SVRCRAGMIGAGLTKCGELRPGTRISDDDVRLIRALRDSDYPRRLSFREIGEKFGISE
                     HSAHAVYYRRRTAEDAVLRELTP"
     gene            14090..14422
                     /locus_tag="OO596_000541"
     CDS             14090..14422
                     /locus_tag="OO596_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716492.1"
                     /translation="MITTLFVESDEPLVCAAGMPLCGGTLTGVYFGDLRGYPWHSLDD
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHLSALP
                     ELQGIHNG"
     gene            14560..14847
                     /locus_tag="OO596_000542"
     CDS             14560..14847
                     /locus_tag="OO596_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716493.1"
                     /translation="MEKEIKFAPKSIDEELAKIGMLERMRDIIEYAIKENLAAREALL
                     IMEREINLIKDAVSLDNKIAREEYVRRRLGVDGSAILTSEHYAKIFNLFQR"
     gene            complement(14828..15067)
                     /locus_tag="OO596_000543"
     CDS             complement(14828..15067)
                     /locus_tag="OO596_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716494.1"
                     /translation="MSDIKNALDVAAFTMAGNLMVTLFDKGLISREEAIHIINLAQKN
                     STPENTEDIQDGSYINTDWHFDNLRACMGITAEKD"
     gene            15138..15650
                     /locus_tag="OO596_000544"
     CDS             15138..15650
                     /locus_tag="OO596_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HCR6716495.1"
                     /translation="MNRASLITLIHVAKRDLQLDHDTYTSALLAATGKSSCRDMSPGE
                     LSRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGGETALNRWV
                     KSQTAAQNDGEGVANWQWLEQQPALASDVLERLKRWHRRKMLAALDMSERAVMSYERV
                     CRLYEKSFPR"
     gene            15721..16143
                     /locus_tag="OO596_000545"
     CDS             15721..16143
                     /locus_tag="OO596_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6716496.1"
                     /translation="MAETQMSMFGDSEQLHALIDRLDDIPDDELKKNWPGTLRDLVDV
                     ISAELHRQGIEPAQAGMLARKVAAAQAGYMGGRGYYLPVGESLFTELRNNEIFSRWSQ
                     GEKIETLRRHYQMSETQIYSVIRNQRRLHQERTQPRLF"
     gene            16215..16715
                     /locus_tag="OO596_000546"
     CDS             16215..16715
                     /locus_tag="OO596_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6716497.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENTTYIDP
                     TGTPTICYGHTGPDVKPGMTKTDEECLELLEKDMKWAFAAIDRHVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            16750..17178
                     /locus_tag="OO596_000547"
     CDS             16750..17178
                     /locus_tag="OO596_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716498.1"
                     /translation="MCRCAAAGCAVSAVNNPLPALIAGGCMAALGVMSVLAAVVWGMH
                     QKTQRLEDNNHVLVRERDEARLVLANQQRTLQLISQISKAATNEKQQNMQHSEEQQSI
                     VRRSLATVPAAAVPVPDDVADRVRRAVCEIRPCAAGADPR"
     gene            17162..17380
                     /locus_tag="OO596_000548"
     CDS             17162..17380
                     /locus_tag="OO596_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716499.1"
                     /translation="MPIPAEWLADCLVPPAPQPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLETQRQHEFTDMAGTTAVPAGKTK"
     gene            17391..17618
                     /locus_tag="OO596_000549"
     CDS             17391..17618
                     /locus_tag="OO596_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCR6716500.1"
                     /translation="MDDSDCAQAVMERATERALCDRLTRKRRVAETPGRRVCADCGGD
                     IPAARLVAVPDAIRCVNCQNIMEAGHVGQHR"
     gene            17599..17910
                     /locus_tag="OO596_000550"
     CDS             17599..17910
                     /locus_tag="OO596_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HCR6716501.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VERVESRISGLPTQQEVNRLNIEITTLRGELKATNATLKSVSHQNELLLEQAVRKKHN
                     E"
     gene            17903..18193
                     /locus_tag="OO596_000551"
     CDS             17903..18193
                     /locus_tag="OO596_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6716502.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            18196..18777
                     /locus_tag="OO596_000552"
     CDS             18196..18777
                     /locus_tag="OO596_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HCR6716503.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQIEFRIRQMLLGKA"
     gene            18788..19036
                     /locus_tag="OO596_000553"
     CDS             18788..19036
                     /locus_tag="OO596_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716504.1"
                     /translation="MATDNRSLMSSAHQKAFSLATQLPPETIKVCRELTELSIAGEIS
                     LEQLHSLLAEAATDELSGVDVMTEELESRIKSILTGKA"
     gene            19036..20700
                     /locus_tag="OO596_000554"
     CDS             19036..20700
                     /locus_tag="OO596_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716505.1"
                     /translation="MNKARETSPSLMQMTPPRKIDLSGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFADESQVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTDQEWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDNFISRAAWLRESEVLTWCEEH
                     LKPLLEKLNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAANARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGGW"
     gene            20700..22289
                     /locus_tag="OO596_000555"
     CDS             20700..22289
                     /locus_tag="OO596_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HCR6716506.1"
                     /translation="MGRIIDTDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTTHTIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIS
                     DPWIREQTGIPQPTPGEAIFRVRQSGNEPAQTDRETPPEKQEKTEQTALSARLPEAKS
                     TPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLADALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDDTGD"
     gene            22273..23604
                     /locus_tag="OO596_000556"
     CDS             22273..23604
                     /locus_tag="OO596_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HCR6716507.1"
                     /translation="MTQGIDLGYAATLPSKEAVAYFRVKGAHISWNWFETAADVHARS
                     FTAAKAARLDVVTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSDGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPSHSAL
                     NGLVFRYDDPFWKTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGEDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMQTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSVKHQNDGIALMPEDFGRLPAMLAKPKAVLWDKRHKNLMYIVENKDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGQID"
     gene            complement(23606..23685)
                     /locus_tag="OO596_000557"
     tRNA            complement(23606..23685)
                     /locus_tag="OO596_000557"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            23726..24199
                     /locus_tag="OO596_000558"
     CDS             23726..24199
                     /locus_tag="OO596_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR6716508.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAESLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYTPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            24377..25501
                     /locus_tag="OO596_000559"
     CDS             24377..25501
                     /locus_tag="OO596_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HCR6716509.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIKGHPAPAAGFVMASPENFRFS
                     EERGFEVRPDWNPPAIEHLRNNEFPWFSPVIGYDEITGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDTLKTQVASLSAELETAKGTPTGGTIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYMEGLGQQIGVAALSAQLDARQPVAALTTLQTD
                     TVTVPDKKTATAALSAEDIAAAHLLGKTEAEFLKMKEEMQ"
     gene            25501..26448
                     /locus_tag="OO596_000560"
     CDS             25501..26448
                     /locus_tag="OO596_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HCR6716510.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYQKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGLNAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMRDFKADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNTTVSNEMKGKLELVVADYL"
     gene            26492..26845
                     /locus_tag="OO596_000561"
     CDS             26492..26845
                     /locus_tag="OO596_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716511.1"
                     /translation="MNEHHTVAATDDSGLQVSGDSPVTVLAEVRCSRAQFRRAGFLFM
                     RGRQQVDVTPEQLARLEAEPCLTVRILQTPGDDAGAVAGVVHAETGADLAEAGQDAPR
                     KKTGNNARQAKKARA"
     gene            26842..27261
                     /locus_tag="OO596_000562"
     CDS             26842..27261
                     /locus_tag="OO596_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HCR6716512.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDARKLNRALTDASA
                     LIDSYLSARYTLPLAVVPAVLVQHCCTIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTDGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            27258..27821
                     /locus_tag="OO596_000563"
     CDS             27258..27821
                     /locus_tag="OO596_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HCR6716513.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLISAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREVSRPGLYQIVARLITVLDGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFTEP
                     EGTPEFKAHISLPGATAAGEKAGPSEE"
     gene            27825..28112
                     /locus_tag="OO596_000564"
     CDS             27825..28112
                     /locus_tag="OO596_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6716514.1"
                     /translation="MTTIFIKPAPGCLIRDPDTMKPLAQEGEEKPFTPFWCRRLNDGD
                     VIVVEKAAEAAPAAASATTDAAGKPAGAAAEKPATEKPATEKPAASDKGTS"
     gene            28112..29614
                     /locus_tag="OO596_000565"
     CDS             28112..29614
                     /locus_tag="OO596_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6716515.1"
                     /translation="MINFDYIGDNNRIPLVQIEINNSMAVTGTPPQRQAVLLFGQAAM
                     KDTTIQGRGQLDVPVRITRASQARELWGRGSMIALMVEEFIAINPDTELYAIAQGAGT
                     GQSRACVMNIIGTALADGVLSVYIGGRRYLLPVSKGKKGKELVEELAKVINADTDAPF
                     TAIAAEVSGDNAEGLKGSMGVNARFIGECSAHDLRVNYYDGETTPAGLTLELVAPTQK
                     ATNPDITRSVSGMGNRQYNYIVMPYKDAANLKILADELLKRWGPVKMSDGMVWMAHTG
                     TFGTATSFGESRNDFLFTCTSIPKAPEPDYVWAASICAVCAPSLSTDPARPLQTLALP
                     ARMAPEAPARLTREERNSLLYTGMSTVTVAAADVVQIERQVTMYRQNAYGESDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPRHKLADNGTPVRPGQNIVTPEIMTLQLIALGEEWV
                     ELGLVENLDTFKKNLLVERNTKDRNRLDVMCTPDLVNQFRFMAAQIRFIL"
     gene            29623..29988
                     /locus_tag="OO596_000566"
     CDS             29623..29988
                     /locus_tag="OO596_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6716516.1"
                     /translation="MSGKQHQGTATIRVNGQEYATLEGATFSPSGFEREVVKGAKVYG
                     YREKPREATLDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGDISAKFASATSTRVQ"
     gene            30003..30482
                     /locus_tag="OO596_000567"
     CDS             30003..30482
                     /locus_tag="OO596_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6716517.1"
                     /translation="MTTRKKKTAVSDEAVMEAIRDVLEDGDPRTAGLAEQLANGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAETEAERLMETRNGGPVLIASPALRGIALLRR
                     QIAAVGQIEGPLSPRQIGQLSERDLSRLMAAVSLLDSAMAGKLVAERGRPDAVPGSD"
     gene            30610..32667
                     /locus_tag="OO596_000568"
     CDS             30610..32667
                     /locus_tag="OO596_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6716518.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDKAKTEAVKAWAVQNAKDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSEAQGASLQLKQMFARQSIQAADAN
                     ILTGYGINVYQLLADKLGVNQKIIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDQFARDVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRAIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLMYMYL
                     ASRALKVLRFAGTGALRLGATPLRYGMAMTSVLTSPFRKPQTTVPGTQPGRAGRFLNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRGRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLTAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     QAWQKLRGSSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNY
                     GLY"
     gene            32654..34012
                     /locus_tag="OO596_000569"
     CDS             32654..34012
                     /locus_tag="OO596_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCR6716519.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPSVAEDTAAALEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDISAFTASFTALKGNAAALINAPRRMAE
                     SLAGMFAVLAGLPGDPSLSLTGKAGTPSGSLTTNRDSTTEQAMPQLYRTLSSLRYTLS
                     EQDDPQRLIGLTPAAQNNIRLLRSVMQSAALVSQAQTVGKLLDQVIRQNTTHVQDTDR
                     AGGLAWLESSADVQRINRDLSDAMEQQVLDLSAQGHTGTALALRDAGLALTEDLTTRS
                     VHLPGAFQVMVRTTEPALVTLYRATGNSRRWQYFVRRNNIPDPVFVPGGRRVEVISEQ
                     QD"
     gene            33996..35120
                     /locus_tag="OO596_000570"
     CDS             33996..35120
                     /locus_tag="OO596_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6716520.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRAARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRPKIILADQQTDITGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSALRFGIEQTELLVCQVEFLLDEHNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            35110..35724
                     /locus_tag="OO596_000571"
     CDS             35110..35724
                     /locus_tag="OO596_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR6716521.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKDGRIIVTCKMLEIYADEGMRVDTPEATFTGNVTVDKNLHVKGNF
                     ALEGTGKSQGLFTMSDAVIAGIRYSGHVHHDNGEGSKTGVPENG"
     gene            35717..36154
                     /locus_tag="OO596_000572"
     CDS             35717..36154
                     /locus_tag="OO596_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6716522.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLLTAVLISLFTDRRALA
                     SDEIPDGTRDRRGWWGDSFRPRPIGSRLWLLSREKTLASVISRARAYADEALGWLSQD
                     GVASSVVCHAERVGHARLALSVRITLPDGSVRPMIFYADLKGE"
     gene            36154..37236
                     /locus_tag="OO596_000573"
     CDS             36154..37236
                     /locus_tag="OO596_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6716523.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYAQAG
                     LSAQEHEHLAWIARQIIPTEADEAELLKHCAFWGVMRKPASRGDGPVQLMLTTDAGIK
                     EGVLLQRSDGVVYRITTSLTGKAGTLNVSVEAESAGRAGNAPAGTKLTFITPQAGINQ
                     TATVTGTGITGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCRP
                     EWPQAGSVGVTFVQDNNPDIFPGDGDVQRVADYIRSHDDPATGQPVGQPLGPTVTVFK
                     LTNKPVPFSIRIIPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKNLED
                     FEVRSPLTSVQAGDSELLTVGEITWL"
     gene            37227..37787
                     /locus_tag="OO596_000574"
     CDS             37227..37787
                     /locus_tag="OO596_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR6716524.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLASLCLGLSHSTARV
                     SWTGKQLLAERFPDTSRLLLEDWERYLGLPECDMAGATLTERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYQIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            37787..38668
                     /locus_tag="OO596_000575"
     CDS             37787..38668
                     /locus_tag="OO596_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6716525.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENKGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNRAANALPSDGTAVAANRLANAHTINGVPFDGTQD
                     ITISSGTVTAIRLGSVTAHMPGTWESWDLNLGGGNVLTGIKVQDVGKNTADNVGGVYY
                     RPLQYLLNGAWVTAASI"
     gene            complement(38700..39221)
                     /locus_tag="OO596_000576"
     CDS             complement(38700..39221)
                     /locus_tag="OO596_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6716526.1"
                     /translation="MELKNITRYYPENMPYGNNVQYFQSEDGKDFYESLPLFTKKYKL
                     CITPDSGVICSISQDASALYPAGFSVVEVDELPEGTDISGNWKFDNGIISRIPVNYAR
                     KLEAMRQSYLNQAYEKINDWRTELQLGTISDEDRAALTQWMAYISQVKKMELPAIKTE
                     AEFNAIKWPEQPQ"
     gene            complement(39273..39596)
                     /locus_tag="OO596_000577"
     CDS             complement(39273..39596)
                     /locus_tag="OO596_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6716527.1"
                     /translation="MPSDGTAVAANRLATPRNINGVPFDGTQDINITSGITQQDADSR
                     YIQDIRLGAENRQVMYGGAYYSAYGNVMAGLQIDSKVDASNDFIAFRPLQKLVGGTWI
                     TVSQL"
     gene            39712..40284
                     /locus_tag="OO596_000578"
     CDS             39712..40284
                     /locus_tag="OO596_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6716528.1"
                     /translation="MLIGYIRVSTNDQNTALQRNALECAGCELFFEDRISGKTRERPG
                     LKRALKRLRPGDTLLVWKLDRLGRSMQHLVLLTEELRQRGVNFRSITDNIDTSTPMGR
                     FFFHIMGALAEMERELIIERTRAGLAAARAQGRHGGRPQKLTEKEREQIARLLANGES
                     RKQIALIFDIGLSTLYRYFPASPAPSPKVP"
     gene            40370..42424
                     /locus_tag="OO596_000579"
     CDS             40370..42424
                     /locus_tag="OO596_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6716529.1"
                     /translation="MSDSKWKMLSAMPGEFSVKVAGGTVAFIESPFRPSGNKGGITFA
                     DCVIRFSTKEPLWVMPVSGNPSAEITSSGVTGIIPITSDVAGTLTPSDWNAPDNAGPS
                     GSTNGSSSEPEYYFVVILAGQSNGMAYGEGLPLPDSFDRPDPRIKQLARRSTVTPNGT
                     SCTYNDIIPADHCLHDVQDMSGINHPKADLAKGQYGTVGQGLHIAKKLLPYIPQNAGI
                     LLVPCCRGGSAFTTGADGSFSEASGASADSSRWGAGKPLYQDLVSRTKVALAKNPKNK
                     LLAVVWMQGEADLASGSQQHNSLFTAMVQQFRTDLAPLAAQCVSGNATTVPWICGDTT
                     YYWKNAGTDKYEAVYGGYKGKEAQKIFFVPFLTDENGQSTPTNAPAEDPDIVAVGYYG
                     AASRTQGSFVSTQRDSHFSSWARRGVISDRLASAIMLHAGRTAELMRGQTVTPPDEKP
                     SPDTPSKPSTPPADTTTMSTLFAYRASESEGRLTPQGWAAGGGKAQIVDDAGASGGKA
                     MKLTKETGRAPWYLEHDAGNGADLLGKGGLVSCRFKLDGALTANQYALALYWPVSALP
                     QGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNTAQNTKLGSFGAFNNEWHTLGFRF
                     AGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITKNATYPVLIDSIVVEVN
                     NA"
     gene            42569..42751
                     /locus_tag="OO596_000580"
     CDS             42569..42751
                     /locus_tag="OO596_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCR6716530.1"
                     /translation="MTFLQLILLYFCTAVCALYLVSGGYKAIRNYVRRKIEDAAAEKL
                     SKTAQVTPLPKDPTQP"
     gene            42787..43041
                     /locus_tag="OO596_000581"
     CDS             42787..43041
                     /locus_tag="OO596_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR6716531.1"
                     /translation="MPEIKGTVTEDLVKQALYSGEVNKLLKAQVRKDFEAQIDTYVDE
                     VLAKLIGPAVAASDADNEPKTDAQPEQPDPAQPGTDSTMM"
     gene            complement(43209..43469)
                     /locus_tag="OO596_000582"
     CDS             complement(43209..43469)
                     /locus_tag="OO596_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716532.1"
                     /translation="MIENAEIYNDYFSDYSDACKYSSSAGEFICFNFFIKSYVFPPQQ
                     EDGGVPPPEITLHRIASVSMMEQQANELYSILGTILQKRESD"
     gene            43759..43959
                     /locus_tag="OO596_000583"
     CDS             43759..43959
                     /locus_tag="OO596_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6716533.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSQSATEHP
                     TYTRKSCRGEQKASYIPYHQHA"
     gene            43913..44650
                     /locus_tag="OO596_000584"
     CDS             43913..44650
                     /locus_tag="OO596_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCR6716534.1"
                     /translation="MGNRKQVTSRIISTPELIHYNDNIVGYGSRELRVETISCWLARL
                     VVVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNNGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHETTFYELDGEWYHEICKNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLDKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            44779..44967
                     /locus_tag="OO596_000585"
                     /pseudo
     CDS             44779..44967
                     /locus_tag="OO596_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
     gene            45101..46645
                     /gene="appC"
                     /locus_tag="OO596_000586"
     CDS             45101..46645
                     /gene="appC"
                     /locus_tag="OO596_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6716535.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            46657..47793
                     /gene="appB"
                     /locus_tag="OO596_000587"
     CDS             46657..47793
                     /gene="appB"
                     /locus_tag="OO596_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6716536.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            47806..47898
                     /gene="cbdX"
                     /locus_tag="OO596_000588"
     CDS             47806..47898
                     /gene="cbdX"
                     /locus_tag="OO596_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6716537.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            47978..49276
                     /gene="appA"
                     /locus_tag="OO596_000589"
     CDS             47978..49276
                     /gene="appA"
                     /locus_tag="OO596_000589"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6716538.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(49391..51571)
                     /gene="etk"
                     /locus_tag="OO596_000590"
     CDS             complement(49391..51571)
                     /gene="etk"
                     /locus_tag="OO596_000590"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR6716539.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(51591..52037)
                     /gene="etp"
                     /locus_tag="OO596_000591"
                     /gene_synonym="yccY"
     CDS             complement(51591..52037)
                     /gene="etp"
                     /locus_tag="OO596_000591"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6716540.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(52025..53164)
                     /locus_tag="OO596_000592"
     CDS             complement(52025..53164)
                     /locus_tag="OO596_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6716541.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(53210..55306)
                     /locus_tag="OO596_000593"
     CDS             complement(53210..55306)
                     /locus_tag="OO596_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6716542.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(55306..56052)
                     /locus_tag="OO596_000594"
     CDS             complement(55306..56052)
                     /locus_tag="OO596_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6716543.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(56049..56693)
                     /gene="gfcB"
                     /locus_tag="OO596_000595"
     CDS             complement(56049..56693)
                     /gene="gfcB"
                     /locus_tag="OO596_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCR6716544.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(56800..57105)
                     /locus_tag="OO596_000596"
     CDS             complement(56800..57105)
                     /locus_tag="OO596_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716545.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(57552..57764)
                     /gene="cspH"
                     /locus_tag="OO596_000597"
     CDS             complement(57552..57764)
                     /gene="cspH"
                     /locus_tag="OO596_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6716546.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT        13745 a        14682 c        16141 g        13273 t
ORIGIN      
        1 tttcactacg ctttattaac aatactttct ggcgacgtgc gccagtgcag aaggatgagc
       61 tttcgttttc agcatctcac gtgaagcgat ggtttgcctt gctacaggga cgtcgcttgc
      121 cgaccataag cgcccggtgt cctgccggtg tcgcaaggag aagagacgtg cgatatgaat
      181 aacgaggaaa cattttacca ggccatgcgg cgtcagggcg ttacccggcg cagctttctc
      241 aaatattgta gtctggctgc cacgtcgctg ggattaggcg cgggaatggc accaaagatt
      301 gcctgggcgc tggagaacaa accccgcatt ccggtggtat ggatccacgg tctggaatgc
      361 acctgctgta ccgaatcttt tatccgctcc gctcacccac tggcgaagga cgtcatcctt
      421 tccctgattt ccctcgatta tgacgatact ttgatggctg ccgccggaac ccaggcggaa
      481 gaagtcttcg aagacatcat cacgcaatac aatggcaaat atatcctcgc agtagaaggt
      541 aatccgccgc tgggcgagca ggggatgttc tgtatcagca gcggtcgacc gtttattgag
      601 aaactcaaac gtgccgctgc cggagccagc gcgattatcg cctggggaac ctgcgcgtcc
      661 tggggctgcg tgcaggccgc gtggcccaat ccgacgcagg caacgcctat cgacaaagtc
      721 atcaccgaca aacccattat caaagtacct ggctgcccgc cgatcccgga tgtgatgagc
      781 gccatcatta cttacatggt gacctttgat cgcttgccag atgtcgacag aatgggccgt
      841 ccgctgatgt tctatggtca gcgaatccac gataaatgct atcgccgcgc ccacttcgac
      901 gccggagagt tcgtccagag ttgggatgat gacgctgccc gcaaaggtta ctgcctgtac
      961 aaaatgggct gcaaagggcc taccacctat aacgcctgtt cctccacacg ctggaatgat
     1021 ggcgtttctt tcccaatcca gtctggtcac ggctgcctgg gctgtgcgga aaatggtttc
     1081 tgggattgcg gttcgttcta cagccgcgtg gtcgatattc cgcaaatggg tactcattcc
     1141 accgccgata ccgtcggttt aaccgcgctt ggtgtggtgg cagcggctgt tggtgtgcac
     1201 gcagtcgcca gcgccgttga ccagcgcaga cgtcataacc agcaacctac agaaaccgaa
     1261 catcagccag gcaatgagga taaacaggca tgagcactca gtacgaaact cagggataca
     1321 ccatcaataa tgccggacgc cgcctggtgg tcgacccgat tacgcgcatc gaaggccaca
     1381 tgcgctgcga agtgaatatt aacgatcaga atgtgatcac caatgccgtc tcctgcggca
     1441 ccatgtttcg cgggctggag atcatcctac aagggcgcga cccgcgcgat gcgtgggcgt
     1501 tcgttgaacg tatctgcggc gtctgtactg gcgtacacgc cctggcttcg gtttacgcca
     1561 tcgaagatgc tatcggtatt aaagtgccgg acaacgccaa tatcatccgc aacattatgc
     1621 tggcaacgct ctggtgccac gatcatctgg tgcacttcta tcagcttgcc gggatggact
     1681 ggatcgatgt gttagatgcg ctgaaagccg acccgcggaa aacctccgaa ctggcgcaaa
     1741 gtctctcctc ttggccgaaa tcatcccctg gctatttctt cgacgtacaa aaccgcctga
     1801 aaaaatttgt tgaaggcggg cagttgggga tcttccgcaa tggctactgg gggcacccgc
     1861 agtacaaact gccgccagaa gctaacctga tgggctttgc ccactatctc gaagctctcg
     1921 atttccagcg tgaaattgtc aaaatccacg cggtctttgg cggtaaaaac ccgcatccaa
     1981 actggattgt cggcgggatg ccttgcgcca ttaacattga cgaaagcggc gcggtcgggg
     2041 cggtcaatat ggaacgcctg aacctggtgc agtcgattat cacccgcacg gcggacttca
     2101 ttaacaacgt gatgatcccc gacgccttag ccatcggtca gttcaacaaa ccgtggagcg
     2161 aaatcggcac gggtctttct gataaatgtg tcctcagcta cggtgcgttc ccggatattg
     2221 ccaacgactt tggtgagaaa agtctgctga tgcctggcgg cgcggtgatt aacggcgact
     2281 tcaacaatgt gctgccagtg gatttggttg atccgcagca ggtgcaggag tttgtcgacc
     2341 acgcctggta tcgttatccc aacgatcagg tcgggcgtca tccattcgat ggcattaccg
     2401 acccgtggta caaccccggc gatgtcaaag gcagcgatac caacattcag cagctgaatg
     2461 aacaggaacg ctactcgtgg atcaaagcgc cgcgctggcg cggtaacgcg atggaagtgg
     2521 ggccgctggc acgcacgtta atcgcttatc acaaaggcga tgctgcgacc gttgagtcgg
     2581 tcgatcgcat gatgtcggcg ctgaacctgc cgctttccgg tatccagtca acgttgggcc
     2641 gcattttgtg ccgcgcgcac gaagcacagt gggccgcagg taagttgcaa tatttcttca
     2701 acaagctgat gactaacctg aaaaacggca atcttgccac cgcctccacg gaaaaatggg
     2761 aacctgcaac ctggccgaca gagtgccgtg gcgtcggctt taccgaagcg ccgcgcgggg
     2821 cgttaggcca ctgggccgcc attcgcgatg gcaagattga tctctaccag tgcgtggtgc
     2881 cgaccacctg gaacaccagc ccgcgcgatc ccaaagggca gattggcgct tatgaagcgg
     2941 cgctgatgaa caccaaaatg gcgatccccg agcaaccgct ggagatcctg cgtactctgc
     3001 acagctttga cccgtgcctc gcctgttcaa cacacgtgct gggcgacgac ggtagcgagc
     3061 tgatctccgt gcaggtgcgt taacagcgaa ggagaatcat catgcaacag aaaagcgaca
     3121 acgttgtcag ccactatgtc tttgaagcgc cagtccgcat ctggcactgg ctgacggtgt
     3181 tatgcatggc ggtgttgatg gtcaccggat actttatcgg caagccgcta ccttccgtca
     3241 gcggcgaggc gacgtatctg ttctatatgg gctacatcag gttaattcac ttcagcgccg
     3301 ggatggtttt taccgtggtc ttgctgatgc ggatctactg ggcttttgtt ggcaatcgat
     3361 actcccgcga gctgtttatc gtgccggtat ggcgtaaaag ctggtggcag ggcgtgtggt
     3421 atgaaatccg ctggtatctg ttctggcaaa acgtccgagt gccgatatag gccataaccc
     3481 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
     3541 ctttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
     3601 ggaatttttc tactggacgg gaggcaactc aatggacatt cacagctggc atcggctggg
     3661 gatgtggctg attggcgcgt ttgtgatcgg tcatgtctat atggcgctgc gtgaagacat
     3721 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
     3781 caaaataagt aacaaggagc gttcatgagc gagcaacgcg ttgtggtaat ggggctgggc
     3841 aacctgctgt gggccgatga aggtttcggc gtgcgggtgg cggaacggtt gtatgcccat
     3901 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
     3961 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
     4021 cctggaacgc tgcgaaccta tgccggagaa cgtattccgg cttatctcag cgcgaagaaa
     4081 atgagcctgc accagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
     4141 ctgccagcac atattgccct cgtcggtctg caacccgcaa tgctcgacga ctacggcggc
     4201 agcctgagcg aactggcacg ggagcaactg cccgcagcgg aacaggcggc gctggcgcag
     4261 ctggctgcat ggggaattgt gccgcaaccg gctaatgaat cgcgctgtct caattatgac
     4321 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtacaggat gacactggag
     4381 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
     4441 gctggacgcc ggttagtgaa ttccgtcttg acgactggct tacgcaagcg ccagacggcg
     4501 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
     4561 ttggcgaatt actgcgcgag tttcccggct acacatggca ggtggcgatt gctgaccttg
     4621 aacagagcga agccatcgga gatcgctttg gcgtctttcg ctttcccgcc acattagtgt
     4681 ttaccggcgg aaactatcgc ggtgtgctga atggtattca cccgtgagcg gaactgataa
     4741 acctgatgcg tgggcttgtc gaaccacaac aggagcgtgc ctcatgagcg aaactttttt
     4801 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
     4861 gatcagctgt caggtgaatg atgaaccgag tatggcggcc ctggagcaat gtgctcacag
     4921 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagtcaac gccagccgcc
     4981 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
     5041 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagcga
     5101 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcaaac gtcgcggcga
     5161 aaagttgctg gaggacaaac tggaggccgg ctgcgcgccg ctggcgttgt ggcaggcggc
     5221 cacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
     5281 tggcctaccg ttggcgcatg agttactggc gcatgtacgt aaccccgacg cgcagccgca
     5341 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcacgtct
     5401 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcatgta
     5461 accaggtgaa tcatttgata ccgacaaggg ttttagtgaa aggaggtgaa atcacctgta
     5521 agatgctgaa ataaaacagg tgatgtatac ttcggacgga aaactctttt tgataccgac
     5581 tgctcaacaa atagtcaaaa gatcaaaaaa cggcgacaca tttgatactg ctaatttacc
     5641 cttaaaatct tcttcaaaac atcttcaaac tgccaaaatg ctcacataat cgatctaact
     5701 ataatcagta tgataatcat catgaattac tattcaatag cgtgattatc ttgggatgag
     5761 tcatatgatc tctatatgta tttacagcac cacctcatga tagcgatggc gcttaaccaa
     5821 caacattttt ctttactgtc actgcccctg attcctgggt ggcgttattg ctcgagttgt
     5881 acttctcaat aacgcgcaaa atctctttgt ctgtttcttc acttcccgcc attcctatct
     5941 ttattgcatt ttttagtacg ggacgtaatg gcaaggcatc aatgatgctt tctaccgcat
     6001 cctggctgat tgtagatgta ttcattgctg ccagcctgac aaggttgtta gtaccttctc
     6061 gcctgatgaa tgtaaggatt aattgcttct cttgggggct tagagagcta agcgcctgat
     6121 caatacccga acctgaatcg caattttttc cgatttttcc gcattcatct tcgttcttgc
     6181 ctgtggccaa ccagtcgagt gaacaattac aggctttagc tattaaggcc agtttatcca
     6241 aaggagggaa gtttttcccc ttcctgtaat ttcttactgt ggcttcagat agtccacaaa
     6301 gggtcgccag ttcgacgttt gtcatgtttc ccatagccat agctaagcgt tctgaaaatc
     6361 ggtttattcc ttccatatca ataaaccgtt ccgcactgga tacggttttt tctgccttat
     6421 ccatataacc ccctataaaa caaagaaatt atcgacatcg catttaattt cgatatgctc
     6481 gcaaaaaacc gttgatatcg aattattttg cgattatgat ttacctaaat cgataaccgg
     6541 ttttgattat cggttcaggt aaacatgaga ggatcacaca atgagggtat ccagacaagc
     6601 atcgaataat ttgtttgttc gagaggggtg tgactggtct gctaaagcgg tatatgcagc
     6661 ccttgaaaac aaaggcacag acctccctga actggaaagg gagttaggat taaaaagcgg
     6721 atcaatgcgc aatgtgtttt accgccagtg ccgtaaatac gaagccgcca tcgccgaaaa
     6781 aataggggtt gaacctgctg taatctggcc cagcagatac caggtggacg ccagagaagt
     6841 ggcttgatgg gggatgtatg tgggtaacag caaaagaatg tgttggtctt cctgatttac
     6901 caatgatgga acataacatt cgccatcgac tcacaaaagc agctgcggga aatgcagcca
     6961 tgttacgcaa acgtaagggg acaaaagcaa tcgaatttaa tatcgattgt ttgccttctg
     7021 gcgcgcaaca agtattgcgt gaacgttatg cccttcaact gatgacgcaa aaagctgatg
     7081 aatcaccggc tccggtggtg acaaaggcca gacgctcatc tgccgtggtt gatgcggtgg
     7141 aggcatatcg cggatcacca caactgatgc tcgaacgcct caatgccctg actgaaaacc
     7201 agcgccaggt ggctgatgca cgaatcgcga tcgtcagcga agttctgaaa gtcgcgcaac
     7261 aacctggttt cagctgtgcg aaggctatcc ggtttatcgt tgacaacctg gcacgttcgc
     7321 aactggacga gcgcattgtg gcaatggttg agacggcgaa cgccaaaaag ggaaacagcc
     7381 gtgcgttgag tgaaatcacg ctgaaacgct ggattgcggc ctttaacaag gcacagaacg
     7441 ccgctgaacg cctgctttta ctggcaccgg gtaaacgcca ggaaataaaa gccgaagata
     7501 ttaactggct gcccgaattt ctggcgcagt atcgccagtc aaacggccga ccaatgaccg
     7561 aggcttacga ggattttgtt gctgaatggc agcaccggca cgctgatgag ccttatatgc
     7621 tcgatatcat gccctcttat gacaccattc gccgcgcaat gaagaaactg ccggaagtgg
     7681 tgaaacaaaa aggccgggta actggcagtg aataccgtca gcttgaggga ttcacgcgcc
     7741 gcgactggtc cagaatgccg gtgaattatg tctggattgg tgacggtcac ggcatgaagc
     7801 tgaaatgcgc gcacccggtt cacgggcggc cattcgcacc ggaagtgacc tttgttatcg
     7861 acggtggcac gcgctttgtg gtgggctgga gccttgacct ggctgaaaat gttttcgccg
     7921 tagccggtgc catacagcac ggtattcgcc atcatggcaa accgtttctg tattactcgg
     7981 ataatggctc cggggaaacc gccgacgtcc tggataagga gattgtgggg atactgccac
     8041 gactggggat taatcacccg acagggattg ccggtaatcc gcagggacgg ggcattatcg
     8101 aacggcttaa ccgcacatta ccgatgcgca tagcccgtaa ataccgcacc tatttcggga
     8161 aaggagcaga tcgcgagacg ttacgtaaaa ccaaccgcga tttacgctcg gcattcagtg
     8221 ccctgcaaca gggcaaacgg ctgaacgccc ggcagcagtc agcgatgcgt gatttaccgt
     8281 cctggtctga actgattgat gccattcgtg acggtgttga gtggtacaac aaccggccgc
     8341 acgatgaatt acccatgaag ccgaacggta aacattacag cccggcggag ttcagaaaaa
     8401 aacgcctggc ggaagaggac acggaaattg aatggctgtc cgatgttgaa ctgcgggata
     8461 tgttccggcc gatggtggag cgccctgtaa gacgctgtga aatacgctgg ctgaataata
     8521 tttactacgc gcccgagctg cgtgacgagc atggccgtaa ggtacttatc agctatgaca
     8581 ttcatgatgc cgaacgaatt accgtgcgcc gcccggatgg cagcgtgatt tgcgaggcgg
     8641 tatgggacgg caataaacgc gaagccttcc ctgtcagcgc ggaatactac aaacagcagc
     8701 agcgccttaa aggcatgcgt aaacgcgcag aggaaaaaat ccgtgatgcc gaggatgagg
     8761 ttgtcaacgt gctggagcac aagccgcagg agccctggct ggaaaacata taccgccctg
     8821 tgggtaatac ggtgaccgtt cagcaaccgg tcgctgacga tgaacctgat gaagaatacg
     8881 agcgtaattt ccagcgggga ttgcaactgc tcgaagcgaa attaaaagaa agtgatccgc
     8941 tggcctgaaa taaaaaaata acccgagggg tgactcaggt tatttgaggt ttcagtctcg
     9001 gagaatgctg tctcaagcga ggtgaataat atgaccgata ttaacgatgt aatcaagacc
     9061 attgatgaac ttattgatgg cggcgtactg acgcagtatg ccatcgccag agaggcggga
     9121 atttccgacg gcacattatc ggctttccgt aagggaaaat ataaaggcga taacgctgct
     9181 gtggctgctt ccctgcgctc ctggtatgag aactggaata aacaaagcgc actgccggaa
     9241 ccgccgcagt ttgtggaaac tcagacagtc caggagctgc gcgcactgtt tcaggcggtt
     9301 cgcctgatgg gctgtattaa cgttattgtg ggcgtaccgg gtgtgggtaa aacggccact
     9361 gcccgtaatt actgccagga gcaaccaaac acctggatga tcaccctgtc acccgcgcac
     9421 tccagcgtca cggagtgtct gctggagctg gccgatgcgc tggggattga ttacacccgc
     9481 gcgaacaaag gggcattatc ccgcgccatc cgccgtcgcc tgacgggaac gcgtggactg
     9541 gtgattgtgg atgaggcgga tcatcttggt attgacggtc tggagcaact ccgggcaatt
     9601 caggacgcca cggggatcgg gatggtgctt attggtaacc cgcgcggatt gtttaaaggt
     9661 ggacgccgcg cctttgatga tttatcgcgc ctgttcagcc gtcttgcccg tacaaaacaa
     9721 cttcgcaagg ccaaaaaggc ggatgtgctg gccattgcca gggcatgggg tatcagtggt
     9781 gaggccgagc tggccgtcat gcaggctatc gctgaaaagc cgggagcatt acgtgttctg
     9841 acacatacgc ttaaccaggc gtggctcacc gccagcggtg aaggcgcggc gctgacagaa
     9901 aaacatatta atgcggcctt taaagaggtt tataccaacc ctgaattact ctcacaggtg
     9961 tgattatggc ggtatttaat attcctgata tttacggacg cttttacctg gttaatttcg
    10021 ataacgtgaa ggtgatttca ctggccgaaa ataaagcatg tggcgattta ctttttgaat
    10081 ttaatgaccg cacacgaatg gtgatatctg ccggacttga tcgcgaaggt gcgacagaag
    10141 tttacagcgg aatatgccgt tctgttggtg cgaaacaagt cagctaaatg aggtgttata
    10201 tgaatatgca atcctgcggt aacaaaatga atttattcga ctccctgaac agcgcgcgcc
    10261 gtctgaccga acttgccggt gcggtactgg aacgcagtaa gcgctatccg caacgttttg
    10321 cactgaaaac cacgccgccg gtaggcaacg tgcagggaac cggtgaaatt gaaatcacca
    10381 tacagaccaa cggcctgcgc cgccgtgtga aggccacccg catcagcggc tgcacggttt
    10441 actgggaggt gtgaggtgaa aaaaaatctc attgcatggg cgtggtcgag tggtcttatt
    10501 gagtttggtt acgtcctgcc ggaaggtgca ttgccgatag ttgccggaaa gcccgccacg
    10561 gtacggcatg tgattgatgt tgtggcgcgt catggacgcg atgaacagga acagctactg
    10621 gttcctggaa taccggaagc ggtgacggaa gaagaagcct ttaatgccat ggttcagttc
    10681 tgccgtgagg tcagacgccg ggtcagttat ccaaacagaa cgagggccag aggatgagta
    10741 aagtcgtgcg cattattttc gaatacaagg aggacgttat ccgtaaaaac gctgatggaa
    10801 cagtgcacat gggggtaagt ctggatatac gttcaaccgg aataaagcag aaaaatgata
    10861 gccccgccat gatttttggg gtggttatgc tggcagaaag cagaaacttc gcagagcttg
    10921 tggcaatgaa agccagtgca ttcatgaaag acaggggcat ggcttccggg gttattaacg
    10981 gtaatgaatt taatcagcag gggtaattcc atgagcaaag tacgcgttat ttttgaattt
    11041 gagcatgttt cgcatgacga aaaaccggca ggcaatgaca gtgttgaagt gcatgaaaag
    11101 attggtgtgg atgtaaaaac agaacgtgat acggataaca ggccgacgtc actctgtgac
    11161 gtttatgcaa gtattctcca gtatcacagt cctgaaatta ttcagtttct ctcagcggaa
    11221 tttcaggcat ctgtacaggc ttttggggcg gatgccatca ttaaacgcca ccgcgtgcat
    11281 aaagcatcag gcacactgca ataaggaaaa acaaaatggc taaacgcgtt acaaaattaa
    11341 aggccgcagc cgaggcagca ccgcagaccc gtgaagaggt cagccgcgat atccgacgct
    11401 ttggggatat gcagcgtgag gcactacgcc tggaaacggc gatgaatgac gaagtggcag
    11461 aaatcaccgc ccgttatacg ccgcagattg aaaaccttaa aaaagaaatc aaagtgcttt
    11521 ttaaggggat tcatgactgg tgtaaaacca accgcaatga gctgacgaac ggcggcgaaa
    11581 ccaaaacagc caatctgacc accggaacgg tgtcatggcg gctgggaacg ccatcatgca
    11641 gcgtcagccg tgatgtggaa ggcgtgattg aaatgctgcg ccgtatgggc cttgagcgct
    11701 tcatccgcac gaaagaggaa gtgaataagc aagctgtcct ggcggagcca gatgcggtga
    11761 agggcattgc cggtataaag gtgaataaag gcgctgaaag tttttatgtc gagccttttg
    11821 aacaggacgc cggactgaat aaataacacc gcattaaatc tttaaatatc acatcgtgtt
    11881 aattatggcg ctcgcgtcag gggactgcct gcgcctgtaa actgaaaata aggaacagga
    11941 aatatggcat atttttattt caaattagac atagtgtaca caaataaata tttaaccgaa
    12001 tatcagcggg atgttttacc gctacgcaac agtattcttc gggcattact gaaaaataca
    12061 ggcgctgccg gattgcgctt aaagccgttc gccatggacg taatcagtga gttttatttt
    12121 tctggttctc tgcctgcggg ctggcgtaag cgcgatgatg tggcttttat cggggacgga
    12181 ccgtgcttta ttgccagacc tgatgagtca tgccctgaag gtccggcgat tgccgcaatg
    12241 attgaaaccg ctgaacgtga gttaagaaag cgtcctgatt tccttgtctg gctctgtgaa
    12301 aagctggggg taatgagaat cccctccatg tttaacacgg actcctggtg gaccccgtcg
    12361 ctctcccgtg atgccctgtg cgtggtgttt aaagtaggcg cttatggcag ggaaataaaa
    12421 gggggtattc ctgaagaatg ccaggaaatt aaacattctg aatatgtggc gctgacggag
    12481 gaataattca tgattgatgc aaaagtgctt gaaggggtta aaagctggct gcgtttttct
    12541 ggccgtctga ccagtcgttc tctggcagaa aaaatgaata tgccgctatc ctccatggtt
    12601 tattttctgc gtgatgcggt cgatgccggg gtgctgacgg accgtaacgg tttttatgat
    12661 attccgcgtc cccgcccggt gcaggcggtt cgtcgcaaat gcagccagga aggtgcggct
    12721 gatgatgttc agtggtgcag cttcagaaaa tccctgccgt ggattgaggg gcatgatatt
    12781 ccgtcgatgg cgtgggaatt tgctcaggga gtactgacct gcgaaaccgt ttatgtggtg
    12841 gctgaagttg atgagcaggc catgaaagaa ggcgtgcccc agtttgtgat ggcgtatatc
    12901 gacattcgcc tgggcgtcat tatctgcggc ttaagcggct ggaacatcac cgggcgcgtt
    12961 ctgcgttacc tgattattga ccggacagcg gcatcggcag ggatatctgc ggaggtggcg
    13021 taatgttctt taaaacatca aacccttccg cgctggctgc gtggcaaaaa taccagcagg
    13081 actgccagaa agttaaggat gaggcaaaac gccttgaggc cgtgctgaat gttgcgtgcc
    13141 ggtcggtatt tgtatccggt atcagtggat tttgctttaa gggactgcgt tttacggagg
    13201 acaaatatcc ttttcatcgc gacttatggc gaaaaccgac tgcgtcgaat ggctggagct
    13261 gcacaccgcg cacatcacgt atccctaaaa ccctgcgtgt tgcctctgat gaactgaaca
    13321 gtctgtggcg tgaatattcg cccgtcacgt atgccagaac cgatgcgctg ttgttctggc
    13381 tgggtattga tttctcggca atcctgtttg gccctgtgaa gtggttctgc gttgacgatg
    13441 tgatttacct tcagtgcggc gtaaaacccg caaaacagaa aatgaccgaa atactgtctg
    13501 atgagtttta tgctgctgaa aagcgagtca gggggtgatg catgatgaaa ttacaaccca
    13561 tgggactaaa aggtcgcgcc cccgctcatg tccgtccgtg gacacctgaa gaagatgcgc
    13621 tgctgattgc gctttattca tccaccccgg ttaaggatat tgctgccaga ataaaaagaa
    13681 ctgtctgggc tgtatataac cggactggtg tattgcgcag ttcatacccg gagttactga
    13741 aatataaaca cccaagattt acacctgatg aagataagtt tattcgaaaa aatgccagaa
    13801 caatgacctg ccagcaaatg ggagaatatc tcggacgtaa taaagattct gtcagatgtc
    13861 gggcaggaat gattggtgct ggattaacaa agtgcggaga gttacgcccc ggcacgcgca
    13921 tatctgatga tgatgtacgt cttatacgtg cgctgcgtga ttccgattac ccacgccgtc
    13981 tgtcattccg ggaaattggc gaaaagtttg gaatatctga acattctgct cacgcagttt
    14041 attaccgtcg ccggactgcc gaggacgctg tattacggga gttaacgcca tgataacgac
    14101 cttatttgtt gaatcagatg aacctcttgt gtgtgccgcc ggaatgccgc tctgtggcgg
    14161 aacgctgacc ggcgtttatt tcggggattt gcgcggttat ccctggcatt cactggatga
    14221 cgcatttccg cctgatatgg aggctgtcgt gctgattgtt cagtatggtg accaacagga
    14281 actgcgcatc ggccatatgg ggtatgaagg cttttttatt gatgaagaaa ccggagcctg
    14341 ccttgaagat gaggacggac aggtgacgca ctggtgccat ctttcggcct tgccggaatt
    14401 acaggggata cataatggat gagcaaattg ttgaatgtcc cacctgtgga aatgaggacc
    14461 cggagtatct gaaagagtgc ccacattgtg gtgagataaa atgtaaccac tgcgatatgg
    14521 gcgacgacac tgcatgtata aattgtgagg atgaataata tggaaaaaga aatcaaattt
    14581 gcgcctaaat ctatcgacga agaactggct aaaatcggca tgcttgaacg tatgcgggac
    14641 attatcgaat atgcgataaa agaaaatctt gcagccagag aagccctttt gataatggag
    14701 cgggagatta acctgattaa ggatgctgta tctctggata ataaaatagc ccgcgaggaa
    14761 tacgttcgcc gcaggctcgg tgttgatggt tcagcaattc ttacatctga acattatgca
    14821 aaaattttta atctttttca gcggtaatcc ccatacaggc ccggagattg tcgaaatgcc
    14881 agtcggtatt aatatatgag ccatcctgga tatcttctgt attttccggg gtagagtttt
    14941 tttgagcgag attgataata tgtattgcct cctcacggga aatcagcccc ttatcgaaca
    15001 gggttaccat aagatttcca gccattgtga atgcggcaac atcaagtgca ttttttatat
    15061 ctgacataga tttttccttt tatgttaatt gacatggcgg tgcggtaaca ccgcccattt
    15121 tttatggagc cacgataatg aatcgcgctt ccctgattac cttaatacat gtcgcaaaac
    15181 gcgatctaca gcttgaccat gatacctata catccgcgct gctggcagcc accggcaaat
    15241 ccagttgccg tgatatgtca cccggtgagt tatcccgcgt gctggatgtt ttcaaaaaac
    15301 gcggttttaa agtgcgtcag aagccggtta accgggcctt aaaaccgggt acggtgaccg
    15361 ccaaaattcg cgccatctgg aaagtgatgc accggcaggg atttatctct gatggcgggg
    15421 aaacggccct taaccgctgg gtgaaatcgc agacggccgc gcagaacgac ggcgaaggtg
    15481 tggctaactg gcagtggctg gagcaacaac ccgcgctggc ctctgatgtg ctggagcgtc
    15541 tcaaacgctg gcaccgccgc aaaatgctgg cagccctgga tatgtcggaa cgtgcagtga
    15601 tgagttatga gcgtgtttgc aggctgtatg aaaaatcatt cccccgttaa ccccaaatcc
    15661 cgccacagcg cgggattttt attttaaact tgccctgtgt aatgatgacc ggaggcgggt
    15721 atggctgaaa cacagatgag catgtttggt gacagcgaac agctgcacgc gctgattgac
    15781 cgtctggatg atattcctga tgatgagctg aaaaagaact ggcccggcac gctgcgggac
    15841 ctggttgatg tgatcagtgc tgaactgcac cgtcagggga ttgaacctgc tcaggcggga
    15901 atgcttgccc gtaaggttgc agccgcacag gccgggtaca tgggcgggcg tggttattat
    15961 ctcccggtcg gtgaatccct tttcaccgaa ctgcgcaata atgaaatttt ttcccgctgg
    16021 agccagggcg aaaaaattga aaccctgcgc cgccactacc agatgtcaga gacgcagatt
    16081 tactccgtta tccgcaacca gcgtcgcctg catcaggagc gcacgcagcc ccggcttttc
    16141 tgatattccg caaatcctgc ccccttttcc tcacggttac gctgactcag agaacaccat
    16201 gagacagcgt aacaatgcca aaactccccg cacccctgcg taaaaagctg attgcccttg
    16261 ttctggccgg agccgggacg ttcaccattg ccacgcacta caccggctac tgggaaggga
    16321 aagaaaacac cacgtacatt gatcccaccg gcacccccac catctgttac ggccataccg
    16381 gcccggatgt gaaaccgggg atgacaaaaa ctgatgaaga gtgtctggag ctgctggaaa
    16441 aggacatgaa atgggccttt gcggctatcg atcgtcatgt tcaggtgccg cttacccgtg
    16501 gtcagacggt ggcgctggcc tcctggattt tctgggccgg tgaaacgaac tttcgcaact
    16561 ccacgctcct gcgccttatc aatgccgggc agatgcccgc gtcctgtaag cagtatatcc
    16621 gctggattta ttcaaagggg gtaaaactcc ccggtcttga ggcccgccgt tcggcggatg
    16681 aatggttatg tcgttacgat ttgccgaaag tctgaaccgc ttctggcgac cgctcatgat
    16741 tgcgctgctg tgtgccgttg tgctgctgcg gggtgtgctg tgtctgcggt gaacaatccc
    16801 ttaccggccc tgattgccgg aggctgtatg gcggcgctgg gggtgatgag cgtgctggct
    16861 gccgtggtat ggggaatgca ccagaagacg cagcgccttg aggacaataa tcatgtgctt
    16921 gtgcgtgaac gggacgaagc ccgtctggtg ctggcaaacc agcaacgcac cctgcaactc
    16981 atttcacaaa tcagcaaggc ggccacgaat gaaaaacagc agaacatgca gcacagcgag
    17041 gaacagcaga gcattgtccg ccggtcactg gcaacggtgc ctgctgccgc tgtgcctgtg
    17101 cctgatgatg tggctgaccg ggtgcggcgg gccgtctgtg aaattcgtcc ctgtgccgcc
    17161 ggtgccgatc cccgctgaat ggctggctga ctgcctggtc cctcccgcgc cgcagccgtt
    17221 cacgtttggt gcatcggtca cttacaacct gcaactgctg gcggtgatta aaaactgcaa
    17281 cgtggacaag gccagcatcc gccgtctgga gacacagcga cagcatgaat ttactgatat
    17341 ggccggaacg actgctgttc cggcaggaaa gacgaagtaa ggaaaaaggt atggatgatt
    17401 cagattgtgc tcaggcagtc atggagcgtg caacagaacg cgccctttgt gaccggctga
    17461 cacgtaagcg ccgggtggcg gagacaccgg gcaggcgtgt atgcgcggac tgcgggggtg
    17521 acattccggc tgcccgtctg gttgccgtgc cggatgccat ccgttgtgtg aactgccaga
    17581 acatcatgga ggccggtcat gtgggtcagc atcgttaaag attatgtcgt gccgatcctg
    17641 tcggcaacgg ccacagccgg ggggatcttc atggcgctga tgcgcaaaac ctttgtcccc
    17701 cgcgaggcct ttgaaaaact ctccgaccgg gttgagcggg tggagagccg gatttccggc
    17761 ctgcccacgc agcaggaggt gaacagactc aatatcgaaa tcaccaccct gcgcggtgag
    17821 cttaaggcca ccaatgccac cctgaaatcc gtctcccatc agaacgaact gctgctggag
    17881 caggctgtaa ggaaaaaaca caatgagtga tttcattact gaagatcagc gtcttgtcat
    17941 tctgcggtcg ctggcggatt acaacggcga actgggcgaa tccgtgcttc aggactgcct
    18001 ggatgattac ggtcaccgcg tgtcccgcga caccgttcat acccacattg cctggcttgc
    18061 cgagcaggga ctggtgcgta agcgcacgct tgttaacggt tatttcatcg ctgaactgac
    18121 cggccggggt caggatgtgg cggaaggccg cgccaccgtt ccgggtgtga agaaaccacg
    18181 cgcaaggggg taacgatgga taagccaacg cgtggccgtg tacgcaaggt ggatttgctc
    18241 cccgacagca tccgtaagcc gctgctggaa atgctgcgtg aaaaacgtct gacgcaggtc
    18301 cagatccgcg aggaaatcaa ccgtctgatc cgtgaagcag gtctgccgga agagcagcag
    18361 ctctcaccgg cggccatcag ccgggaagcc tcccgtaatg agctgattgc ccgtaatctg
    18421 cgtgatttac gcgagcagac aaaagccatg atggccgaac tgggggataa gccgaccggt
    18481 gaaaccacgg cactgattct ggagatgtcc cgtgccctga tgtaccgccg tctgcgtgcc
    18541 gccaccgaat ccctgaacag cgacagcgat gtggatatgc gccttattaa ggacatcctc
    18601 ctgtcggcac agcgtgcaga aagtgccgcc gagcgcagca ttaagcgtga aaaagaaatc
    18661 cgggccgcat ttgctgaaga gatggcgaat gcggtcaccg atgagttgcg cggcgtggat
    18721 ggcatgagcg agcagatcga attccgtatc aggcagatgc tgctgggtaa ggcgtaggag
    18781 gtgaatcatg gcaacagata accgctcact gatgagcagt gcccatcaga aagcattcag
    18841 tcttgcaaca cagttacctc ctgaaaccat aaaggtatgt cgtgagttga ctgaactcag
    18901 cattgccggg gaaatatccc tggaacaact gcattcgctg ctggcagagg ctgccactga
    18961 tgagttgagc ggcgtggacg tgatgactga agagcttgag tcccgaatca aaagcattct
    19021 gacaggtaaa gcatgatgaa caaagccaga gaaacgtcgc cttcactgat gcaaatgacg
    19081 cctccccgca aaattgatct gtcaggtgaa aaagccctgc tgggcgtgga tgtgccggac
    19141 agccttgatt tgcccggtga tatgccggtg tttctggatt atcaggcccg ctggtttgca
    19201 gatgaaagtc aggtctgcat cgcggagaaa tcccgccgca ccggtcttac ctgggcggaa
    19261 gccgggcgta acgtcattac tgctgcaaaa ccgaagcgtc gtggtggccg caatgtgttt
    19321 tatgtgggat caaagcagga gatggcgctg gaatatattt ctgcctgcgc gctgttctcc
    19381 cgtgccttta accagctggc agatgctgat gtgtatgagc agacattctg ggatcgggat
    19441 aaaaaagaag aaattttgac ctacatgatc cgctttccga acagtggatt caaaattcag
    19501 gcattgtctt cccgtccgtc aaacctgcgc ggccttcagg gggatgtggt gattgatgaa
    19561 gccgcgttcc acgagtcact ggatgagctt ctcaaggcgg caatggcgct cactatgtgg
    19621 ggcgcgcgcg tgcgtattat ctccacgcat aacggcgtcg ataatctgtt taatcagtac
    19681 attcaggaag cccgcgaggg gcgcaaggat tacagcgtcc accgcataac cctggatgat
    19741 gccattgcgg acgggctgta ccgtcgtatc tgttacgtca ccgatcagga atggtcaccg
    19801 gaaagcgagc agaaatggcg tgatgatctc tacaaaaacg ccccgacccg tgaggatgcc
    19861 gacgaggaat acggctgtat cccgaaaaaa tccggcggtg cctatatccc ccacgcgctt
    19921 attgaaatgg cgatgatccg cgacatcccg attctgacgt ttgaagcccc ggacaacttc
    19981 atcagccgtg ccgcatggct gcgtgaatcg gaagttttaa cctggtgtga agaacattta
    20041 aaaccgcttt tagagaaatt aaatccccgt tcccgcttca gcttcgggga agactttgcc
    20101 cgcaccggtg acctgtcctg ctttgtgctg ctggaaatca ccgaatccct ggcaaagcgc
    20161 gaggtgtttc gcgtggagct gcgcaacctg ccgtatgccc agcaggaaca ggtgatgatg
    20221 tacatcctga cccgcgttcc ggcgctggtc ggtgcggcgt tcgacgccac cggtaacggc
    20281 ggctatctgg cagaggccgc actgctggcc tttggcccgg atatcatcga ctgcgtgatg
    20341 ttgtcgccga agtggtacgg cgagtggatg ccaaaactga aagccgagtt tgaagatcag
    20401 aatatcctcg tcgcccgcca tcagaccacg cttgatgatt tgcgccatgt gaaggtagtg
    20461 aatggtatcc cgcaaatcga caaggggcgc acgaaggacc agaacgcaac ggctgcgaac
    20521 gcccgccgcc atggtgattt tgccgtggcg ctctgtatgg caaaccgggc gtcatacatg
    20581 gagggcttca tcctggatga atcagcctgt caggcgctac cggagcggtc gcgggcaatg
    20641 gagggcggtt atcgtgatga cgatgaggca tatcatgaat ttgatcgggg gggctggtag
    20701 tgggacgcat cattgatact gacggaaaac ctttctcctt tgacccggag atgcagagtg
    20761 ccgcgctgga tattccgcag attgccagcc gttatattga acatccggct tcgggtatca
    20821 ccccgaaccg ggcggcgcag tgccttcgcg gggctgaacg tggcgatctg attgcccagt
    20881 ccgatctggc cgctgatatt gaagaaaagg acacccacct ttttgcggag ctgggcaagc
    20941 gacgtcttgc cattcagggg gtgccatgga gtattgagcc gccaccgaac gccagcgcga
    21001 atgagaaaaa ggacgcggaa atgctcgacg aatatctgca ttccgcagac tggtttgatg
    21061 ccatgctgtt tgacgccacg gatgccatcc tgaagggcta ttcctgcatg gaaattgagc
    21121 acgggatgct cggtaaaatg cacatcatcc gcgccatccg ctggcgtgac agcgggcatt
    21181 tctgccttaa cccggatgat ttgagcgaac tgcggctgcg tgacggcagc catgccgggg
    21241 tggcgtttca gccttttggc tggatagtgc atcagtcacg ttcacggacc ggttacggtg
    21301 gcgcaacggg gcttgtcagg acgcttatct ggccgttcat tttcaaaaac tattccgtgc
    21361 gcgatctggc cgaatttctg gaggtgtacg gactgccgat gaaggtcggg aaatacccgt
    21421 ccggggcaac accggagcag aaaagcgccc tgatgcgggc ggtgatggat atcgggcgac
    21481 gtacaggcgg gatcatcccg gccgggatgt cgctggaatt tcaggcggca gcaaacggtc
    21541 aggccgatcc atttgaaacc atgatttcgt ggggggagcg ttccatctcc aaagcaatac
    21601 tcggcggcac gctgaccacg gaagccggag acaaaggcgc gcgctcgctg ggtgaggtgc
    21661 ataacgaggt gcgccgggaa atcagggatt ctgatttacg gcagctggct gcaaccctga
    21721 accgcgatct ggtgtatccg ctgtacgccc tgaacaccac gcacactatt gatatccgcc
    21781 gtctgccacg tatctgcttc cagaccaaag aaccaggtga tatcaccaaa atcaccagtg
    21841 cggtgatgca gctcagtacg gggatggata tttctgatcc ctggatacgg gagcagaccg
    21901 gtattccgca gcccacgccc ggtgaagcca tattccgtgt ccgtcagagt ggcaatgaac
    21961 cggctcagac cgacagggaa acaccaccgg aaaaacagga aaaaacagag cagacggcgc
    22021 tgtcagccag actgccggaa gcgaaaagca cgccgcgtga tgaactggat gatatggggg
    22081 acgcggtgcc tgcccgccgc ttacaggagg ccatcgaccc ggtactggag ccggtcattg
    22141 atgccatcag aacgcggggg ctggcggatg cgctggcaga cctgcctgcc ctttatcgtg
    22201 aaatggatga ttcccgcctg atgacgctgc tcagtgatgc catgtttgct gcggaaatga
    22261 aggggatgct ggatgacaca ggggattgat ttaggttatg ccgccacgct tccctcaaaa
    22321 gaggcggtag catacttccg tgtcaaaggg gcgcatatca gctggaactg gttcgaaaca
    22381 gccgcggatg ttcatgcccg ctcattcacg gcagcaaaag cggcacgcct ggatgtggtg
    22441 accacgttac aggccgaagt gcagcgggcc attgatgagg gaatttcaca gaaagcattt
    22501 atccgcacgc tgacgccacg cctgcaaaag ctgggatggt ggggaaagca gattgtggtg
    22561 gacagcgacg gtaacgcgga agaagtgcaa ctgggcagtc cccgtcgtct ggcgctgatt
    22621 tacaacgtga acacccgtgt ggcttacaat gccgggcgtt acacgcagat gatgaacaac
    22681 acggacacgc atccgttctg gcagtatgtg gcggtcatgg acagccgtac ccgcccgtcg
    22741 cattccgccc ttaacggtct ggtattccgc tatgacgatc cgttctggaa aacacactac
    22801 ccacctaatg gctggaactg ccgctgccgt gtgcggccat tgtctcaggc tcgtctggat
    22861 gcaatggggt tatccgtttc atccggtgag gatcatctct ccacccgcaa tgttgaggct
    22921 ggcgtggata aacagaccgg agaagtcaga gaaatgccgg tgaccacata ttcagatggc
    22981 accagaacca tgacaccgga tgtgggctgg tcatataacc ccggctcggc agcgttcggc
    23041 acagaccagg cgctgatccg caaactgatc gaggtgaaaa gcccggcgtt acgggaaatg
    23101 gtggttcagg agatgaacaa cagcccggag cggcagctgg cgttccgcat ctgggcaaaa
    23161 aacatcatgc agacccggcg aggtggtaac gatatccgca cgctgggctt tatgactgaa
    23221 agcattgcgc aggcagtgga aagccggacg ggaacgccac cggcccgcct gctggcgatg
    23281 agcggtaaaa atgtgctcca tgcagacagt gtgaaacatc agaatgacgg catcgccctg
    23341 atgccagagg acttcgggcg cttaccggca atgctggcaa agccaaaagc ggtgctgtgg
    23401 gacaaacggc acaaaaatct gatgtatatc gtggaaaata aggatggcag tgtgcagatt
    23461 gccgttaatg caccgtacag tctgaaacgt cagcccgaca aactggatgt gattgtgaat
    23521 gcttaccggg ttatcaatat ggataagctg aaatcggata tccggggcgg aatgcttgag
    23581 gtgctggaag gacaaattga ttaacggtca gtggcggggg tcgaacccgc atacatgatc
    23641 cacccggagg caggcaccga ctttaccagt taagcgtaca ctgaccgcct gtttatttta
    23701 tattcatcct ttatgcggag gcaatatgtc atccattgat gccgctgtcg ttgttgacgt
    23761 tacgcgtctc cagcgggtgt ttgcccggct tcagtttgtg ggcggcggaa aagacctggc
    23821 ccgcagtgtg gcggagagtc ttctgtcatc gtcagaaatg gcgtttgaac aggaaaaaga
    23881 gccggacggc gaacgctggc atgactggtc agatccttac cgcaagtggc gtacccgtaa
    23941 gggatacacg cccggcaaaa tcctgacact gaacggcgat ctggcccgcc gcctgaccac
    24001 ggattatggc gatacctggg cgctgattgg atcaaatgaa ccttatgcgg ccattcatca
    24061 gtggggtggc ctgcctggta tgccaccggg accggcggcc attggcgcac gtccgtatat
    24121 gggctttgat caggtggcag aacaggagat catggacgaa atcagaaaac gctttaaaaa
    24181 ggccacagaa acgccgtaat ggtttaaggt atacaaacgc attaccttac cctcttcagg
    24241 cgcgtgtcgt gattttctaa cctgtattta acgggcttta aaatctgcgt gaagcgcctt
    24301 tctcattttt cccctgtcag cactttctga agtcccgcaa aaccggcccc gaaaaacacc
    24361 ggcttatgct gccggaatga agacgaaaaa cacgcccaaa cttgcttatg ccattctgaa
    24421 tgccatcagc ctgtccgcag acggggacgg tgactggtgt cagatcatgc cagccggtcg
    24481 ggtaaaggcc cgtgacggtc gcccggaaaa accggcggaa ggctggctga ttaaccatgc
    24541 ggccgttgag cgcatggtat cccgggttgt ggcgctcaat cagccggtaa aaattgacta
    24601 caaccaccag accctgatta aggggcatcc ggcaccggct gccggttttg tcatggcctc
    24661 accggaaaac ttccgcttca gtgaagagcg gggttttgaa gtgcgtccgg actggaaccc
    24721 tccggccatt gaacacctgc gcaataacga atttccctgg ttttccccgg tgattggtta
    24781 tgacgaaatc accggtgaac ctgttgagct tcggatgctg gctattaccg gtgaccccgg
    24841 tctgactggc atgaatcctg tcgccgcact gtcggcggat gacctttata acgccttaaa
    24901 ccctccttta aaggacacct ccatgaatga gcaattacgc cagttgctga cggcgctcgg
    24961 tctgaccgtg gccgacggtg acgaatttac accggaactg ggcacggcgg cgctgtctgc
    25021 ccttaccggg atcaaaaccc gtgcggatgc gcacgacacc ctgaaaacgc aggttgccag
    25081 cctgtcggca gagctggaaa ccgcaaaagg cacaccaact ggcggcacta ttgatctgac
    25141 gaaatacgtg cccgttgaga cgtataacgc cctgcgtacc gaatatgtcg cgttgtcggc
    25201 acaacacggc agcaccacgc tggagcagtt gctggataag gccgagtccg aaggacgcat
    25261 tttcaaaagc gaacgcggat acatggaagg actcggacaa cagattggtg ttgcggcact
    25321 ttctgcacag cttgacgccc gccagccggt tgcggctctg accactcttc agaccgacac
    25381 cgtaaccgtg ccggataaaa agaccgccac cgctgcgctg tcggctgaag atatcgccgc
    25441 cgcccatctg ctgggtaaaa ccgaagccga gttcctgaaa atgaaagagg aaatgcaata
    25501 atgcctaccc cgatcacacc ggcgatgatc accgccctga tgacgggcta ccgttctgat
    25561 tttcaggccg ggatgtccat ggctccgtct cagtaccaga aaattgcgat gaccgtaccg
    25621 tccacgtcga aatccaacac ctacggctgg ctggggcagt tcccgcagtt ccgtgagtgg
    25681 atcggctccc gcgtcatcga gaagatgaaa gcctatggct acgccatcgt gaacaaaacc
    25741 tttgaaggca ccgttgccat tagccgcgat gactttgaag acgacaatct tggcatctac
    25801 tccccgttgt tccaggagat ggggcgcgct gcggcggcac aaccggatga actggtcttt
    25861 gctgcactgc gtgatggtct gaatgcggcc tgctatgacg gccagaactt ctttgatacc
    25921 gaacaccctg tttaccccaa agtggatggc tccggtgatg cacagatggt cagcaatatg
    25981 tttgtggcaa aaaccggctc tgtcggtgca caggcggatt acagcggccc ggcctggtat
    26041 ctgcttgact gctcccgcgc ggtaaaaccg ctgatttatc aggatcgccg caaggccgaa
    26101 ctggttgccc agacaaaagt cgatgaaggc cgtgcgttta ctgataacga gtttgtgttc
    26161 ggtgcttccg cccgtcgcaa tgtgggctac ggcttctggc agatggccta catgatgcag
    26221 tcaccgctga cgctggatgc gctgtggcac ggctggtcag ccatgcgtga ctttaaggct
    26281 gacggtggcc gcaaactcgg catcaaaccc acccatattg ttgtccccac ctcgctggaa
    26341 aaacaggcgg tgcagctgct ggagcgcgag ctgttcgcgg acggtaacac caccgtctct
    26401 aacgagatga agggcaagct ggagctggtt gtcgcggatt atctgtaaac ggtgagccgg
    26461 gcttacggcc cggcccctgc ggagggcaaa tatgaatgaa catcacactg tggcagcaac
    26521 agatgattca ggccttcagg tcagtggtga cagtcctgtc acggtactgg cagaagtgcg
    26581 ttgcagtcgt gcgcagtttc gtcgtgcggg gttcctgttc atgcggggac gccagcaggt
    26641 tgacgtcacc ccggagcagc ttgcccgact ggaagcggag ccatgcctca ctgtacgaat
    26701 actacagacg cctggtgatg atgcgggagc cgtggcgggt gtggttcatg cagagaccgg
    26761 tgcagattta gccgaagccg gacaggatgc tccccggaaa aagaccggca ataacgccag
    26821 acaggcgaaa aaggctcgcg catgaattac gccaccgaaa ccgatatgcg ggcacgttac
    26881 cgcgaggatt tgctcaggcc attactggcc gtgccccgtt cggatgagcc ggacgcgcgc
    26941 aagctgaacc gggcactgac ggacgcgtct gcccttatcg acagttacct gtctgcccgt
    27001 tacacgctgc cgctggcggt ggttccggcg gtccttgttc agcattgctg cacgattgcc
    27061 ttttattacc tgtgcgatca gcgggcatcc gatcaggcgc gtgaccgtta ccgtgaggcg
    27121 ctggcctggc tgaaagatgt catgaacggt aacgtgccgg tcggcgtgga tacagacggt
    27181 gcggcccctg aatccgggga tttaccgcag gtgcagtctg atgcggcggt gttcgggcgc
    27241 aaccagaagg gctttatatg attacggaaa ccgagcaagc gtacatcgcc cgtatccgtg
    27301 agtatttcgg gaatgaactg gtgtcagttg acacgcatcc cggcgactgg agcgacagtg
    27361 tgctgcgcac catgctgatt agcgccccgg cgatctacgt tgcctggctg ggtgccggtg
    27421 aaggccgtac ccgtgggcgt ctggtcagcc actgggtgtt ctacgtcatc ggcgacatgc
    27481 tcaatggccg tgaggtcagc cgtcccgggc tttatcagat tgtggcccgc ctgattaccg
    27541 tgctggacgg cttcagaacc gaaaaaacct caccgcttta ctttgaaaag gcggtcaacg
    27601 gttacaccga aacgcaggcc gacagtggcg cggtgatgta tgcgctttat ttctcctgcg
    27661 aggaaatgat cgcaccgctg actgatatca gctcgctgga tgatttcctg cgccattacg
    27721 aaaccttcac cgaaccggaa ggcacaccgg aatttaaggc gcacatcagc ctgccaggtg
    27781 caaccgccgc cggtgaaaaa gccggaccgt cagaggaata acagatgaca accattttta
    27841 tcaaacccgc gccgggatgc ctgatccgcg atccggacac catgaaaccg ctggcgcagg
    27901 agggagagga aaaacccttc accccgttct ggtgccgccg tcttaatgac ggggacgtca
    27961 ttgtggtgga aaaagcggct gaagcagcac cggcagcagc gtctgcaacg actgatgccg
    28021 ccggaaaacc cgcgggagcg gcagcagaaa aacctgcgac tgaaaaaccc gcgactgaaa
    28081 aacccgcagc atcagacaag gggacgtcct gatgattaac tttgattata tcggcgataa
    28141 caatcgcatt ccgctggtac agattgaaat caataattca atggccgtga ccggcactcc
    28201 accacagcgt caggcggtac tgttgtttgg tcaggctgca atgaaagaca ccaccattca
    28261 ggggcgcggt cagcttgatg taccagtacg tattacccga gcttcacaag caagagaact
    28321 ctgggggcga ggttccatga ttgcactgat ggtagaggaa tttatcgcca ttaaccctga
    28381 cacagagtta tatgccattg cacagggggc tggaacaggt cagtccaggg catgcgtgat
    28441 gaatatcatt ggaacagcat tggcagacgg ggtgttaagt gtttacatcg gcggtcgccg
    28501 ttacctcctg cccgtcagta aggggaaaaa aggtaaagag cttgtggaag agctggctaa
    28561 ggtcattaac gctgacactg atgcgccatt cactgctatc gcagctgaag tcagtggtga
    28621 taatgctgaa ggacttaaag ggagcatggg ggttaacgcc cgctttatcg gtgaatgctc
    28681 agctcatgat ttacgggtga actattatga cggtgaaact acaccggccg gtcttacgct
    28741 ggagctcgtc gcgccaacac agaaggcaac aaaccccgat atcacccgta gcgtctcagg
    28801 gatggggaat cgtcagtaca actacatcgt catgccctat aaggatgcgg caaatctcaa
    28861 aattctggcg gatgaactgc tgaaacgctg gggaccggta aaaatgtcgg acggtatggt
    28921 ctggatggcg cataccggca ccttcggtac ggcaaccagt tttggtgaat cccgtaatga
    28981 cttcctgttt acctgcactt ctattcctaa agcaccagag ccggattatg tctgggcggc
    29041 ctccatttgt gcggtctgtg cgccctccct gtcaacggat ccggcccgac cattacagac
    29101 actggcgctg cctgcccgta tggcacctga agcaccggct cgtctgacgc gggaagagcg
    29161 aaacagcctg ctttataccg gcatgtccac ggtgaccgtt gcggccgctg atgtggtgca
    29221 gattgaacgt caggtgacga tgtaccgtca gaatgcatac ggcgaaagcg atcccagcta
    29281 tctggatgta gaaaccatct acaccctgtc atatctgcgt tattccctgc gcacctttat
    29341 cacgcagcgt ttcccgcgcc acaaactggc cgataacggt actcccgtcc ggcccggtca
    29401 gaatatcgtg acgccggaga ttatgacgct ccagctgatt gcactggggg aagaatgggt
    29461 ggaactcggg ctggtcgaaa atctggacac ctttaaaaag aatctgctcg tggagcgcaa
    29521 cacaaaagac cgtaaccgtc tggatgtgat gtgtacaccg gatctggtta accagttccg
    29581 cttcatggcc gcgcagatcc gtttcatttt gtgaggtaag gcatgagcgg aaaacagcat
    29641 cagggcacgg ccaccatccg tgtgaacgga caggagtacg ccacccttga gggagccacg
    29701 ttctccccgt ccggctttga gcgtgaagtg gtgaaaggcg cgaaagtcta tggctatcgt
    29761 gagaaacccc gtgaggcgac gctggactgt aaatttccgg ctggcggaga aggttcaccg
    29821 gctgccgatg aaatcaacac ctggactgcg gtcacgattg agttcgtggc cgataccggt
    29881 gaagtccata tgatgacgaa ggcctggagc agtgaaccgg cctcgcttga cggtggcggt
    29941 gatatctccg cgaagttcgc cagcgccacc agtacccgtg tccagtaatc aggaaaaatg
    30001 caatgaccac acgtaagaaa aaaacggcgg tttctgacga ggccgtgatg gaggctatcc
    30061 gggatgtact tgaagacggc gatccccgca ccgccgggct ggctgaacag ctggcgaacg
    30121 gctatgtgga tctgctcgac ggtctgccgt tcggggaatc ccgtgaatac cgcgtcacct
    30181 tccgcgaact gacggcgaaa gacagcattg acgcggaaac cgaggccgag cgtcttatgg
    30241 aaacccgaaa tggcggcccg gttctgatag cctcgccggc actgcgcggc attgcactgc
    30301 tgcgccgtca gattgccgct gttggccaga ttgaggggcc attgtctccg cgtcagatcg
    30361 gacagctcag cgagcgcgat ctctcccgtc tgatggcggc ggtaagcctg cttgattccg
    30421 cgatggcagg aaagctggtc gctgaacggg ggcgaccaga tgcagtgccg ggatcagatt
    30481 gaagaagccg caattgtgct ggggatggtg acaaaaagcg gcccggagtg ggcgcttaac
    30541 ctccccttat cgcagcttta ccggcactgc cgacagaccg aaaaaattct cagaacgaag
    30601 cagtaaccca tggcaaaaaa cctgaaagcc tccctgattg tcgatttact cggcaatatt
    30661 tccgccaaat cccgccagtg gtcacaggag ctgggggcat tctcccgttc cggcaggacg
    30721 gggctgggtg gtcttggaaa tgcagcacgc cgggccggtc aggaaacaga gcttgttggc
    30781 tcccgtatgc agcgaacgct tgccggtgtt cgtggcagta tccgcacggt aacgtcagat
    30841 tttgaccgcc tgcagggcag cattaccggc accatcgggc ggataagcaa cctttacggg
    30901 atgctggcgg gaggggctgc ggtctacggt tttaataaag cctttatccg cccggccgca
    30961 gagatggaga actatattct gcgtctcaac gccattaatc atggcgataa ggcaaaaacg
    31021 gaagcagtga aagcctgggc ggtacagaac gccaaagaca ccacctgggg gctggcgggg
    31081 gttatgcagg aatacgcctc cagtcgcggg tttggcatga gtgacaggga agcccgtcgc
    31141 tttattacca tgcttcagga tcagggcggc tatcacggct ggtcactgtc agaggcacag
    31201 ggtgcgtccc tgcaactcaa acagatgttt gcccgccaga gtatccaggc agcggacgcc
    31261 aatattctga ccggatacgg gattaacgtt taccagttac tggccgataa gctgggtgta
    31321 aaccagaaga tcatccgtga gaaaggcgag aaaggaaaac tgggaccgga cagcattcgc
    31381 ctgttatttc aggtgatggc cgaacaggca aaaggtgccc agaaaaacgc catgaactca
    31441 tggacgggaa tgacgtccat gatgggtgac gtctgggatc agtttgcccg tgacgtgatg
    31501 gcgaagggac cgtttgacag cctgaaaaag agcctgaaag gctttctgga ttatgccgat
    31561 gcggcacaga aaagcggctt acaggataag ctggccacac agacggcctc agcactgaat
    31621 cagggatttg agtatgccag agatgctgcg accggctttt accgggccat tcagaaagtc
    31681 agggaaacgc ttcaggcact gcgcgacgcc ggttatggcg atgcgctgga tcgtattggt
    31741 cagggtgcgc agaccgccgc aaagtacctg atgtatatgt atctcgcctc ccgtgcgctg
    31801 aaggtgctca ggtttgcagg gacgggggca ttacgtctcg gcgcaacccc cttacgctat
    31861 ggcatggcga tgacctccgt gctgacgtcg cccttccgca aaccgcagac cactgtaccg
    31921 ggaacgcagc ccggacgcgc tggccggttc ctgaatttcc ttaccggggt aaatccggct
    31981 gccgttcagc ccgtgctggt caccaactgg cctgcgggtg gcctggcctc aggtggcggg
    32041 gatgtggtgg tcagcggtga cggcaaaacc gtgcgcggcc gtaaaaaacg cggccccgga
    32101 cgtggacggg gtgtcacgac cgtcgtgact gccggtgagc aactggcgga gagtgccggt
    32161 aaacagggct tcttcggtcg tatgatgagc cgtgccggtg gtctgctaac ggcggccgga
    32221 aaccgcatgg ggctggggcg ttttgccggg ctgttccgtg gtgccggtcg gctgggtggt
    32281 ggtgccctgt gggccggtgc catggcggct cctgtactgc tggacagcag cgccagtgca
    32341 gccgataaag ccggtgccgt gggttctctt gccggaagta tcgccggtgg cgcgctgggg
    32401 gcggctgccg gtccggtcgg ggttgccatt ggttccacgg tgggcagcta tctcggtgat
    32461 tatctggggg gctggctgac gcaggcctgg cagaaactgc gtggcagcag tgacgaaaac
    32521 ggcggacagg ccaccgcgaa aaccgccgca cgggtggagc ttgtggcccc tgaaggctgg
    32581 cgggcgcgca gtattgatgt ggatgatacc gcacagcatg gcctggatgt gaacgtctgg
    32641 aacggaggga actatggcct ctactgacac gccggggcgt ggctcctttc gtggcgtacc
    32701 gttcctggtt tatcaggagc agcgggaacg gggcggacgt aacattgtgc gccgtgaata
    32761 cccactgcgt gaaagcggcg gtgcggatga tctggggcca aaactcccgg agttcacatt
    32821 cacggttcta gtgaccggag acgatcttca gacacagcga agccgcctgc gtgatgcgct
    32881 gcgtgcaccc ggtgccggtg aactgatgca cccggattac ggcacgttaa acgtgctgat
    32941 aaacagcttt gaaagccgtt ataacgccag cgagcaggga acggttgagt tcacgatcaa
    33001 cgtgatcccc gccagtgacg ataccgcccc ttcggtggca gaagatacgg cagctgcgct
    33061 ggagcagaaa agcggctcag caatgaatca gctgtttaac acactttcag acggctggac
    33121 ggtgatttca gacggtctgc atgatgtaca ggccatgacc gacaccatca gcgataaaat
    33181 tgatgcgctg gagaatgccg tttcagggat ggggattgtg caggatatca gtgcctttac
    33241 ggccagtttt actgccctga aagggaatgc cgccgccctg attaatgcgc cccgtcgcat
    33301 ggctgaatcg ctggccggaa tgtttgccgt gctggcgggg ctgcccggcg atccttccct
    33361 gtcgctgacc ggaaaagccg gaacgccgtc cggcagcctg accacaaacc gtgacagcac
    33421 gacagagcag gccatgccgc agctgtaccg cacactttct tccctgcgtt acaccctcag
    33481 cgaacaggat gatccgcagc gcctgattgg tctgacgcct gcggcacaga acaatatccg
    33541 tctgctgcgg agcgtcatgc agagtgcagc cctggtgtcg caggcgcaga cggtcggaaa
    33601 actgctggat caggtcatcc ggcagaatac gacgcacgtt caggatactg accgtgcggg
    33661 cggactggcc tggctggaaa gttctgctga tgtgcagcgt atcaaccggg atttgagcga
    33721 tgccatggaa caacaggtgc tggatctgtc cgcacagggc cataccggca ccgcccttgc
    33781 attacgtgat gcagggcttg cgctgacaga agatttaacc acgcggagcg tgcatcttcc
    33841 cggtgctttt caggtcatgg tgcgaaccac cgaaccggca ctggtcacgc tgtaccgtgc
    33901 caccggtaac agccgccgct ggcagtattt tgtccgcaga aacaatatcc ccgatccggt
    33961 ttttgttccg ggtggccgcc gcgtggaggt gatcagtgag cagcaggatt gaactgtata
    34021 tcggcggcag tattttttcc ggctggctga cggtcagcgt ccgtcgctcg ctggagcatc
    34081 tggccgggtc gtttgaactg gggctgatgc tgcccggtga gcgcatcccc tcagccctgc
    34141 ggaccggtca gtccctgacg ctcagaatta acgggcagac ggtcatcagt ggctggctgg
    34201 atcaggtcag tcagcggatc agtgcaaccc gtcatcagat cagcatcagc ggtcgggata
    34261 aaaccggcga cctggtggac tgtgccgcca tccatcccgg cagccagtgg cgcaaccgga
    34321 cgctggcaca gattgccgca gatttatgtg cgcccttcgg gatagccgtg cgctggcagg
    34381 ttaacgacga cacggctgca cgccccttca gctcttttac gctggaaaac tcagaaaccg
    34441 tggcggatgc gctgacccgt gctgcccggc atcgcggggt gctggtgacc agtaatgctg
    34501 acggcgatct ggtgttcacc caggcaggaa gccagcagac ggacagactg gtgctgggag
    34561 ataacctgct tgatgccgat tacaacacgg actggcgagg acgatacagc gaataccgtg
    34621 tccggggaca cgggcgcggt ggtggcaaac ggggagacag cgaatccgcc gcccgtctgg
    34681 cagcacctgt gggcgtcatc agtgatgagc agatcggccg ctaccggccg aaaatcatcc
    34741 tcgccgatca acagacagac atcaccggtg cacggcagcg tgccctgcgt gaaatgcgcc
    34801 gtgcgattgc ccgttcagaa cggttttctg ccaccgtgcg tggctggttc cgggatgatg
    34861 gccggttatg ggatgtcaat ctgctgaccg gtgtttcagc cctgcgtttc ggtatagaac
    34921 agactgaact gctggtctgt caggtggagt ttttacttga tgagcataac ggggaagtca
    34981 cccggctggt actggcaccg cgtgacggct ttatcgttcc ggcagagccg gacagtaagg
    35041 gccggggtgg ttccggtgac gatgttgatg cctttattcg ccagcagatg aaaaaacagg
    35101 ggatcagctt tgatgaatga tgaagtgttc agccgcctga ttgccccggt aacgcgcggt
    35161 attcgcctgt tgtttggccg gggcgttctg accggcacac atgacgaact gaaaatgcag
    35221 aatgtgcagc tcaccggcat ggacggcgaa acctttgatg atgtggagcg tccccagcag
    35281 tacgggcaga tcagcgttcc cctgccgggt gcggaaacct tttttgcctg tctgggcgga
    35341 cagcgggatc agactgtggt gcttgtggtg gaagaccggc gcagccgccc gaccggactc
    35401 acagccggag atacgggggt gtatcaccat gaggggcacc ggatacggtt aacaaaggat
    35461 ggccgcatta ttgtgacatg caaaatgctg gagatttacg ccgacgaggg gatgcgggtg
    35521 gatacgccgg aagccacctt tacgggcaat gtgacggtgg ataagaacct gcatgttaag
    35581 ggtaatttcg cgcttgaggg tacggggaaa tctcagggac tgttcacaat gtcggatgcc
    35641 gttattgcag ggatacggta ttccggccat gtgcatcatg ataacggcga aggcagtaag
    35701 acaggagtgc cggagaatgg ctgatattgc aattgtgtgg gatcagggat gcggttcgct
    35761 gcaactgaac ggtgccgacc tgctgacgga tgacagtctg ctgacggccg ttctgatttc
    35821 gctgtttact gaccgccggg cgctggcatc ggatgaaatc cctgacggta cgcgcgaccg
    35881 ccggggatgg tggggagaca gttttcgccc gcgccccatt ggctcccgtc tgtggcttct
    35941 cagccgggaa aaaacgctgg cctcggtgat aagccgtgcc cgtgcttacg cggatgaggc
    36001 gctgggctgg ctcagtcagg atggcgtggc gtcatccgtg gtctgtcatg cagaacgtgt
    36061 gggacacgcg cggctggcgc tttcggtgcg cataaccctg cctgatggtt cagtgaggcc
    36121 catgattttt tatgctgatc ttaaggggga gtaatgcctt atcagccttt accgctggca
    36181 cagttaatca cgcagacaca gcaggatatc agccagcgcc tgcccggctc gcagccgggt
    36241 gtgaatgaaa ccaccctgaa tgccattgct tacgctcagg cagggttatc tgctcaggag
    36301 catgagcatc tggcctggat tgcgcgtcag atcatcccga ccgaagccga tgaagccgaa
    36361 ctgctgaaac actgtgcatt ctggggcgtc atgcgtaagc ctgcctctcg gggggacggg
    36421 ccggttcagc tgatgctgac cacggatgcg gggatcaagg aaggtgtgct tctccagcga
    36481 agtgatggcg tggtttaccg tatcaccacc tccctgaccg gtaaggccgg tacgctgaat
    36541 gtcagtgtgg aagccgaaag tgccggtcgt gcggggaatg ccccggcagg gacaaaactg
    36601 acatttatca caccgcaggc ggggatcaac cagacggcca cggtgaccgg aacggggatc
    36661 accggtggtg cggatgtgga aaccgtgccg gagctgcttt cccgtctggt tttccgggtg
    36721 cagaatccac cgtccggcgg cacgcagtat gattttgaac gctgggcgcg tgaagtgccg
    36781 ggcgtgacgc gggcatggtg ccgcccggaa tggccgcagg cgggcagcgt gggggtaacg
    36841 tttgttcagg ataataaccc ggatattttc cccggtgacg gtgatgttca gcgggtggcg
    36901 gattatatcc gcagtcatga tgatccggcg accggccagc ctgtcggaca gcctctgggg
    36961 ccgacggtga ccgtgtttaa actgaccaat aagccggtgc ccttcagcat caggatcatc
    37021 ccgaaaacac cggagaatca ggcggccgta aaacaggcgc tgaccgacct gttgtataac
    37081 gaatcccggc ccggcggtct ggttctgccg tcgtctttct ggcgggcggt ggcaggggtg
    37141 aaaaatctgg aggattttga agtgcgcagt ccgctgacgt cagttcaggc gggagacagt
    37201 gagctgctga cggtaggaga aatcacatgg ctgtaaccct gaccccgcat cagcgcgccc
    37261 tgttgcagtt actgcctgac gggctggcat gggacaagcg tccgtcatcc gtactggcat
    37321 cattatgtct gggcctcagt cattcgacag cgcgtgtatc ctggacgggt aaacagctgc
    37381 ttgccgagcg tttccctgac acgtcgcgcc tgctgctgga agactgggag cgttatctcg
    37441 ggcttcccga gtgtgatatg gccggagcca ccctcacgga gcgacagcgt tatgccggaa
    37501 ataaataccg gatgaagccc tcgctgaacc gtgaatttta tatccggttt gcggcggagt
    37561 ttggttatca gattgacatt cagccctcac cggattcgca gtgggtcagt attgtcacga
    37621 ttaacagtga aaccggctac cggaatatga atgtgctgga tgatattctc acgccgctgc
    37681 gtatttatga aggcggtgcg ctggaatgta ttctgaaccg ttataagcct gcatggcaga
    37741 cgtttattta cgtttatgca aacagccatg aagaggagac tatttaatgt ttcatgttga
    37801 taataattcc ggcgtggcga atatgcctgc gctggcaccg gcgcagagta ataccaccac
    37861 ctggtttacc gaaggtgacg gacaaaaagg tatcagctgg attggtcagg actggctgaa
    37921 tattctccag gccgaactgc tgaatattct ggctgaagcc agtattcagc cggataaggc
    37981 gcagttaaac cagcttacgc tgtccattaa agccattatc gctgcgaatg ccttttcccg
    38041 gaaaaataac ctgaaagaaa ttgctgatgc cggtgcggag gcccagcgtc tggcccgtgg
    38101 ttatcttggt ctggggacgc ttgccacaaa aaacagtctt ggtcccgctg acgttaatgc
    38161 cctggcgaag gatcagaatc tcgccgacct ggagaataag ggaaccgccc gtaataatct
    38221 ggatgtttac agcaaaagcg aaggtgataa ccgttacctg cgcagggagc agaacggcgc
    38281 agacattccg gataaagggg cttttatcga taacgtcggt ttacgggaaa cggtaaacag
    38341 ggcggcgaat gccctgccat cggacggcac cgccgttgcc gcgaacaggc tggcaaatgc
    38401 ccacacaatt aatggtgttc cctttgacgg gacgcaggat atcactatct catcaggaac
    38461 agtaaccgcc ataagactgg ggtcggtgac cgcacatatg cccggaacat gggaaagctg
    38521 ggatctgaat ctggggggcg gcaatgttct taccggaatt aaagtacagg atgtcggtaa
    38581 gaatacggca gataacgtcg gcggcgtgta ttaccggccg ttgcagtatc tgttaaatgg
    38641 agcatgggtg acggctgcaa gtatataatg atattcagaa ggccttccgg ccttctttat
    38701 tactgtggct gctcaggcca tttaatggcg ttaaattcag cttctgtttt aatggcgggt
    38761 agttccattt tcttcacctg actgatgtat gccatccatt gggtaagtgc tgctctgtct
    38821 tcatcgctga tggtgcccag ttgtagctcc gttctccagt cattaatttt ttcatatgcc
    38881 tgattaagat atgactggcg cattgcttcc agttttcttg cgtagttaac cggaatacgg
    38941 gagattatgc cattatcaaa cttccagttt cctgaaatat ccgtgccttc agggagttca
    39001 tcgacctcaa ccacagaaaa ccccgcagga tacagcgccg aggcgtcctg tgatattgag
    39061 cagataacgc cgctgtcagg tgttatgcaa agtttatatt ttttggtgaa taatggcagg
    39121 gattcgtaga aatccttacc atcttcactc tggaaatact gaacattatt accatatggc
    39181 atattttcag gataataacg ggttatattt tttagttcca taaaatcctc ctggaaataa
    39241 atctgtttta aaatatatta tttactgact atttataact gagataccgt aatccatgta
    39301 cctccgacta atttttgcag gggtctgaat gctataaaat cgtttgaagc atcaacttta
    39361 ctgtcaatct gcaggcctgc cataacatta ccgtacgcag agtaatatgc cccaccgtac
    39421 attacctgcc tgttctctgc tcccagcctg atatcctgga tatatcggga atccgcatcc
    39481 tgctgtgtta taccagaagt gatgtttatg tcctgcgtcc cgtcaaaggg aaccccatta
    39541 atatttcttg gggtggcgag tctattcgcg gcaacggcgg tgccgtccga tggcagagca
    39601 ttcgccgccc tgtttaccgt ttcccgtaaa ccgaggtttt caggaaagcc aggattaact
    39661 ctgttttatt cctcatgcga caattccggc tttttataat aaggaaatcg tatgctgatt
    39721 gggtatatca gggtgtcaac aaatgaccag aatacggcgc tacaaagaaa tgcgctggaa
    39781 tgtgcaggat gtgagctgtt tttcgaagac agaataagcg gtaagacaag ggagcgtccc
    39841 ggacttaaac gcgcattaaa gcgactccgg ccaggggata ccctgctggt ctggaaactg
    39901 gatcgccttg gacgcagtat gcagcacctt gtccttttaa cagaagaact ccggcaacgc
    39961 ggcgttaact tccgcagcat tacggacaat attgatacat ccactcccat gggacgtttc
    40021 tttttccata taatgggcgc gctggcggaa atggagcggg aactcattat tgaacgtaca
    40081 cgcgcgggtc ttgctgctgc ccgtgctcag gggcgtcatg gcggcagacc tcagaaactg
    40141 acagagaaag agcgggagca aatcgcccgt ttgctggcta atggcgagtc ccgtaaacaa
    40201 atcgcactga tatttgatat tggcctttcc accctttatc gttattttcc tgcctcacca
    40261 gctccgtcac caaaagtacc gtgacgcttt tttgaatttc cgcaaaacac atatttttca
    40321 gcggtcgcta ttctgactct gtttaattca gaaacaggca gaaaagacga tgtcagactc
    40381 caagtggaaa atgctctccg ccatgcccgg agagttttca gtcaaggttg ccggtggcac
    40441 ggtggcattt attgaaagcc ctttccgccc ttcgggtaat aaagggggga tcaccttcgc
    40501 agactgcgtg atccgcttca gcacgaaaga gccattatgg gtaatgccgg tatccggtaa
    40561 tcccagtgca gaaatcacca gttcaggcgt gactggcatc attccaatca catctgacgt
    40621 ggccggaacg ctcacgccct ccgactggaa tgcaccggat aacgccgggc cttccggtag
    40681 cacgaatggc agcagttcag agccggaata ttactttgtt gtcattctcg cgggtcagtc
    40741 aaatggcatg gcctacggcg aggggctgcc gttaccggat tcgtttgacc gcccggaccc
    40801 acgcattaag caactggcac gccgcagtac ggtaacgccc aacggtacgt cgtgtacata
    40861 caacgacatc attccggcag accactgcct gcatgatgtt caggatatga gcgggataaa
    40921 ccacccgaaa gctgatctgg caaaaggcca gtacgggact gtcggtcagg ggttgcatat
    40981 tgccaaaaaa ctgcttccct atatcccaca gaatgccggg atacttctgg tgccctgttg
    41041 tcgtggcggg tcagcattca ccactggagc cgacggctca ttcagtgagg ccagcggtgc
    41101 ttccgctgat tcttcacgct ggggggccgg taaacctctg tatcaggacc tggtaagccg
    41161 caccaaagtc gcgctggcga aaaacccgaa aaacaaactt ctggctgtgg tctggatgca
    41221 gggagaggca gaccttgcct caggaagcca gcagcataat agtttattca cggccatggt
    41281 tcagcagttc agaactgacc tggctccgct tgctgcgcag tgcgtgagtg gaaatgccac
    41341 cacggtgccg tggatttgcg gtgataccac gtattactgg aaaaacgctg gcaccgataa
    41401 atatgaggca gtatacggtg gctacaaagg caaggaagca cagaaaattt tctttgtacc
    41461 gttcctgacg gatgagaatg gacagagcac gccaacgaat gctccggcgg aagacccgga
    41521 tattgtggct gtcgggtatt acggtgcagc atcccgaacc cagggcagtt ttgtttcgac
    41581 acagcgtgac agccatttca gctcatgggc acgcaggggc gtcatttcag accgtctggc
    41641 ctcagctatt atgctccatg cagggcgcac ggctgaactg atgcgcgggc agaccgtgac
    41701 accaccggat gagaagccgt cacctgatac accatcaaaa ccgtccacgc cacccgcaga
    41761 caccacgacg atgagtacgc tatttgctta ccgggcatct gagtctgaag gacggttgac
    41821 accacagggt tgggctgccg gaggtggcaa ggctcaaatc gtggatgatg ccggagccag
    41881 cgggggtaag gcaatgaaac tgaccaagga aacaggaaga gccccctggt accttgagca
    41941 tgatgctggt aatggtgcgg acctgctggg taaaggtggt cttgtcagtt gtcgttttaa
    42001 actcgatggc gcgcttacgg ctaatcagta cgcactggcg ctgtactggc cggtttctgc
    42061 cctgccgcaa ggggttactc tggaaggaaa tgccggtcat aacctgctgg catcgttcta
    42121 cgtacagagc gatgccacag accttaacgt gatgtatcac aaaggaaaca cggcgcagaa
    42181 cacgaagctg gggtcattcg gcgcatttaa taacgaatgg catacgctgg gcttccgttt
    42241 tgccggtaac aacagtattg aggtgacgcc ggtcattgat ggtaaggacg ggacaccatt
    42301 catgctgtcg cagtctccgg tcggcacgtt tacggcagac aagttgcgcg tgaccgatat
    42361 cactaaaaat gcgacttacc cggtactgat tgacagtatc gtggtggaag tgaataacgc
    42421 gtaagcagga taaaaaaatg ccgccggtaa gggaaaacaa gaaaccggcg gcaaaaagat
    42481 cgttatatcc aaagcaaaaa atgcagtgca cctttttacc taacagatat taccggtgtc
    42541 aacaccatgt caataattgg gagggataat gacattttta cagcttattt tattgtattt
    42601 ctgcacggca gtatgtgcgt tatatctggt gagtggtggt tataaggcca tcagaaatta
    42661 tgttcgccgc aaaattgagg acgcagcagc tgaaaaactc agtaaaacag cgcaggtaac
    42721 tccgctccca aaagacccga cccagccctg attgcagggc tttattcccg cataaggagg
    42781 atatacatgc cagaaattaa aggcaccgtc actgaagacc tggtaaaaca ggcactgtat
    42841 tccggtgaag taaataagtt gctgaaagcg caggtccgca aggattttga ggcacaaatc
    42901 gacacttacg ttgatgaagt gctggcaaaa cttattgggc cagcagttgc ggccagtgat
    42961 gctgataatg agccgaaaac cgacgcgcag ccagagcaac cagacccggc tcagcccgga
    43021 actgacagta ccatgatgta acaatacaaa ccggtggtgt cctctgccgg tttgacacta
    43081 atgaaaacat ctttgccgct atccagccat atgtaatcgc tggatagtat ttcgtattca
    43141 gaaatatagc tgcttatgga gcagtcaatc ctgcttcccc atccgggcag gcaaatgaac
    43201 cggtatcatt agtccgattc acgcttttgt agaatcgttc ccagaatgct atacagctca
    43261 ttagcctgtt gctccatcat tgagacggag gctatacggt gcaaagtgat ttcaggaggt
    43321 ggcacacctc catcttcttg ctgtggagga aaaacataag atttaataaa aaagttgaag
    43381 catatgaact ctcccgcact cgaactgtat ttgcaggcat ctgaataatc ggaaaaataa
    43441 tcattataga tttccgcatt ttcaatcatt tttcgatttc tcgtcgttac atgattgtcc
    43501 atagcagcct cttacgctgg caattaacca attgacttct ataaattagt tgatttgttt
    43561 gctttgcggt attcaaatgt acaatcattc agcacttatg cgttacagca ccgtttaaaa
    43621 ttagtgatca aactcataaa ttttcgctaa tgacatttcg tgttatttgt tgtatcaatt
    43681 aaataaatca aatcgatcgt ttttgtcgat cgattgagtg ataaagatat caacaacaaa
    43741 gcaggaggtt atgcggccat gtatcgaaac atacggtgcc gacattgtaa caagctactg
    43801 gcacgagcca gcttcagtta tctggaagtt aaatgtccgc gctgtaaaac cctcaatcaa
    43861 atcacatctc agagcgccac agagcacccc acatacacaa ggaaatcctg tcgtggggaa
    43921 cagaaagcaa gttacatccc gtatcatcag cacgcctgaa cttattcact ataacgacaa
    43981 tatcgtcggt tatggctccc gtgagttacg ggttgagaca ataagttgct ggctggcccg
    44041 gctggtcgtc gtcaataaac attacagcca tcgtttcgta aataattcct accttcatct
    44101 gggcatattt tcagaacggg agctggttgg cgtaatgcaa tggggttatg cccttaatcc
    44161 aaacaatggt gcacgcgtcg taactggcac gcagaaccgc gaatatatgg agcttaaccg
    44221 gttatggctg catgactgta tgccgagaaa ctccgaatca agggcaatca gttacgcgct
    44281 caaactaatc agacaacttt atccgcaggt gcagtgggtt cagtcgtttg cagatgagcg
    44341 ttgtggctgt cttggtgtcg tgtaccaggc aagcaatttt gattatgtag gcagtcatga
    44401 aacaacattc tatgaacttg atggcgagtg gtatcacgag atttgcaaga atgcaatcaa
    44461 acgaggagga cagcgaggtg aacatctgcg ggctaatatc gacagagcca gtgttcataa
    44521 attccgtcag tttcgttaca tccggtttct ggacaagaga gccagaaaac gccttaacac
    44581 aaagctattc aaagtccagc cttacccaaa accacaaaca gttaaaactg gtttgaaaga
    44641 gagtgaatga acgcttaaaa actcattcta aaactcaaaa gaaaagcgcc gatttagtat
    44701 caaataagtt gcgtatcggt gtttttcagc ggtatcaaat gaaatgcaaa tcggtatcaa
    44761 aaaaatcgcc gcgctataat caacgccacg gggttacgcc atgtctggca tttacgctgt
    44821 acggacacct taaaaggtcc tttactggaa agttatgaaa tctgcccaat accggaagtg
    44881 gtgctggcag cgccagaaga tttggtcgac tctgcgcagc ggcttagcga ggtatgtcag
    44941 tggctggcgg aagctgcacc gacgtaaaaa gacggtaagt atcgctttca gtcttatgaa
    45001 tatcgcaatc ggcgaatacc tctggtcgta gagtttcagg ataaagaggg agatctaaca
    45061 ttatcgggtt atttttctct cttcgcctac aggagtgcgc atgtgggatg tcattgattt
    45121 atcgcgctgg cagtttgctc tgaccgcgct gtatcacttt ttatttgtac cccttaccct
    45181 ggggctgatt tttttgctgg ctatcatgga aaccatttac gtggtcaccg gcaaaacaat
    45241 ctaccgcgat atgacgcgct tctggggtaa gctcttcggt atcaactttg ctcttggcgt
    45301 ggctaccggc ctgaccatgg agtttcagtt tggtactaac tggtcattct attccaacta
    45361 tgtgggcgat atttttggcg caccgctggc gatggaagca ttaatggcct tcttcctcga
    45421 atccaccttt gtcgggcttt tcttcttcgg ctggcagcgg ctgaataaat accagcacct
    45481 gctggtgacg tggctggtgg cgttcggttc aaatctctct gcattgtgga tattgaatgc
    45541 caacggttgg atgcaatacc cgaccggtgc gcattttgat atcgacaccc tacgtatgga
    45601 gatgaccagc ttcagcgagc tggtctttaa cccggtcagc caggtgaaat ttgtgcacac
    45661 cgtaatggcg ggctacgtga ccggggccat gtttattatg gcgatcagcg cctggtattt
    45721 actgcgcgga cgggagcgcg atgttgcatt acgctcgttt gccatcggtt ccgtcttcgg
    45781 tactctggcg attatcggca cgctacaact cggagacagt tctgcgtatg aagtcgcgca
    45841 agtacaaccg gtaaaactgg cggcgatgga aggggagtgg caaacggaac ctgctcctgc
    45901 accgttccat gtggttgcct ggccggaaca ggatcaagag cgtaacgcct ttgccatcaa
    45961 aattcccgcg ctgttaggga tcctcgccac tcactcatta gataaaaccg tgccgggtct
    46021 gaaaaatttg atggctgaaa cctacccacg cttgcaacgc ggacgtatgg cctggctgtt
    46081 aatgcaggaa atatcgcaag gcaatcgtga gccgcatgtg ttgcaggcat tccgggaact
    46141 ggaaggtgac ctgggctacg gcatgttgct ctcccgctat gcgccggata tgaatcatgt
    46201 cacagccgca cagtaccagg cggcgatgcg tggcgcgata cctcaggttg cgccggtatt
    46261 ctggagtttc cgcatcatgg tgggctgtgg ttccctgccg ctactggtga tgctgattgc
    46321 gcttgtccag acgctgcgtg gcaaaatcga ccagcatcgc tgggtgctga aaatggcgct
    46381 ctggagtttg ccgttgccgt ggattgcgat tgaagccggg tggtttatga ccgagtttgg
    46441 tcgtcagccg tgggcgatac aggacatctt accgacatac tccgcgcact ccgctttaac
    46501 cacaggacaa ctggctttct cactgatcat gatcgtaggg ctttacaccc tgttcttaat
    46561 cgccgaagtc tacctgatgc agaaatatgc ccgtctgggg ccgagcgcga tgcagagtga
    46621 acaaccgacg cagcaacagg ggtaaagggg aaaatcatgt ttgattatga aacattgcgc
    46681 ttcatctggt ggctgctgat tggcgtgatc ctggtggtct ttatgatctc cgacggattt
    46741 gacatgggga tcggctgtct gctgccgctg gtggcgcgta atgatgatga acgccggata
    46801 gtgataaaca gcgttggtgc acactgggaa ggcaaccagg tctggttgat cctcgctggt
    46861 ggggcattat ttgccgcctg gcccagagtg tatgcagcgg cgttttccgg cttttatgtg
    46921 gcgatgatcc tggtgctgtg ctcactgttc ttccgcccgc tggcctttga ttatcgcgga
    46981 aaaatcgccg atgcacgctg gcgtaaaatg tgggacgccg gtctggtcat cggcagtctg
    47041 gtgccgccgg tagtcttcgg tatcgccttc ggcaacttgt tgctcggcgt gccgtttgcc
    47101 ttcacaccgc aattacgcgt ggagtatctc ggcagcttct ggcaactgct gacgccattc
    47161 cctttattgt gcggattgct cagccttggg atggtgattt tgcaaggtgg cgtctggtta
    47221 caactgaaaa ctgttggtgt gattcatctg cgttcacagc tggccaccaa acgcgctgca
    47281 ctgttggtga tgctgtgctt tttgctggcg ggttactggc tgtgggtcgg tattgatggc
    47341 tttgtactgc tcgcccagga tgctaacggt ccttccaatc cgttaatgaa actggtggca
    47401 gtgctacctg gtgcctggat gaataatttc gtcgaatcgc ccgttttgtg gatcttcccg
    47461 ctgctggggt tcttctgccc attgctgacg gtgatggcga tttatcgtgg tcgcccgggt
    47521 tggggatttt taatggcgtc attgatgcaa tttggcgtga ttttcacggc aggcatcacg
    47581 ctgttcccct ttgtcatgcc gtcaagcgtg agtccaatct ccagcctgac gttgtgggac
    47641 agcacttcca gtcagctgac gctgagcatt atgttggtaa tcgtgctgat atttttgccc
    47701 attgtgttgc tctacactct ctggagctac tacaaaatgt ggggacgcat gacaacagaa
    47761 actctccgcc gtaacgaaaa cgagttgtac taaggagcag gaacaatgtg gtatttactt
    47821 tggttcgtcg gcattttgtt gatgtgttcg ctctccaccc ttgtgttggt atggctggac
    47881 ccgcgtctga aaagttaacg aacgtaggcc tgatgcggcg cattagcatc gcatcaggca
    47941 atcaataatg tcagatatga aaagcggaaa catatcgatg aaagcgatct taatcccatt
    48001 tttatctctt ctgattccgt taaccccgca atctgcattc gctcagagtg agccggagct
    48061 gaagctggaa agtgtggtga ttgtcagtcg tcatggtgtg cgtgctccaa ccaaggccac
    48121 gcaactgatg caggatgtca ccccagacgc atggccaacc tggccggtaa aactgggttg
    48181 gctgacaccg cgcggtggtg agctaatcgc ctatctcgga cattaccaac gccagcgtct
    48241 ggtagccgac ggattgctgg cgaaaaaggg ctgcccgcag tctggtcagg tcgcgattat
    48301 tgctgatgtc gacgagcgta cccgtaaaac aggcgaagcc ttcgccgccg ggctggcacc
    48361 tgactgtgca ataaccgtac atacccaggc tgatacgtcc agtcccgatc cgttatttaa
    48421 tcctctaaaa actggcgttt gccaactgga taacgcgaac gtgactgacg cgatcctcag
    48481 cagggcagga gggtcaattg ctgactttac cgggcatcgg caaacggcgt ttcgcgaact
    48541 ggaacgggtg cttaattttc cgcaatcaaa cttgtgcctt aaacgtgaga aacaggacga
    48601 aagctgttca ttaacgcagg cattaccatc ggaactcaag gtgagcgccg acaatgtctc
    48661 attaaccggt gcggtaagcc tcgcatcaat gctgacggag atatttctcc tgcaacaagc
    48721 acagggaatg ccggagccgg ggtggggaag gatcaccgat tcacaccagt ggaacacctt
    48781 gctaagtttg cataacgcgc aattttattt gctacaacgc acgccagagg ttgcccgcag
    48841 ccgcgccacc ccgttattag atttgatcaa gacagcgttg acgccccatc caccgcaaaa
    48901 acaggcgtat ggtgtgacat tacccacttc agtgctgttt atcgccggac acgatactaa
    48961 tctggcaaat ctcggcggcg cactggagct caactggacg cttcccggtc agccggataa
    49021 cacgccgcca ggtggtgaac tggtgtttga acgctggcgt cggctaagcg ataacagcca
    49081 gtggattcag gtttcgctgg tcttccagac tttacagcag atgcgtgata aaacgccgct
    49141 gtcattaaat acgccgcccg gagaggtgaa actgaccctg gcaggatgtg aagagcgaaa
    49201 tgcgcagggc atgtgttcgt tggcaggttt tacgcaaatc gtgaatgaag cacgcatacc
    49261 ggcgtgcagt ttgtaatgca taaaaaagag cattcagtta cctgaatgct ctgaggctga
    49321 tgacaaacga agaactgtct aatgcgtaga ccggaaaagg cgttcacgcc gcatccggcc
    49381 actttcagtt ttactctttc tcggagtaac tataaccgta atagttatag ccgtaactgt
    49441 aagcggtgct ggcgcgttta atcacaccat tgaggatagc gcctttaata ttgacgcctg
    49501 cctgttccag acgctgcatt gacaaactca cctctttggc ggtgttcaag ccaaaacgcg
    49561 caaccagcag gctggtgcca acagaacgcc ccacgaccgc ggcatcactc accgccagca
    49621 tcggcggcgt atcgacaatc accagatcgt aatggtcgtt cgcccattcc agtaattgac
    49681 gcatccgatc gcgcatcagc agttcagacg ggttaggtgg cacctgaccg cgagtaatca
    49741 catcaaagcc tcctttgcca aaatgctgga tcactttgtt gagctcatct ttacctgcca
    49801 gatattccga caagccatgt tcattactca cggtaaacag gttatgcgaa taaccacggc
    49861 gtaagtcggc atcaataaat aacacttttt gatcggactg ggcgatcacc gctgccagag
    49921 ttgaactgac aaacgtttta ccactgtctg gcgtcgcacc ggtgatcatc agaatgttat
    49981 tctccgtctc catcatagcg aaatgcagac tggttcgtag cgcacgtacg gcttccacag
    50041 cagaatccgc cgggttatcc accgccagga aggggatatt tttagtacga tggcgctgct
    50101 gattagaaaa taaatttttc ttacgcagac gggtgcgttt atccagccac tcggacattg
    50161 ggatagtggc ataaacgctg atgccgtgct cttccagttg ttccggggct tctacaccac
    50221 gacgcaacat cgcacgcgcc agcacggcac ccacagaaat aaacaggcca agaataaaac
    50281 caagcaccac attcaacgct tttttcggtt tcactggctg cggctgagtg actgccgggt
    50341 cgataatccg cacgttacca atggcactgg atttcgaaat actcaactcc tgctggcggt
    50401 taagtaattg cagatatacc gcacggcccg cttctacgtc acgacttaaa cgcaacactt
    50461 cctgttgggt ggaaggcatt gccgataccc gcttattcag gcgtttgcgt tcttgctcca
    50521 gcgtctggcg tttttccagc agcgcacgat aagttgggtg atctttctta tacagctggg
    50581 agatctctgc ctcgcggaag gtcagctcat tgagttgatt atcaacgttc acaatctgct
    50641 caagaacggc tttggcttcc aggttaaggt caaccgaatc gcgctgctgg cgataaacgt
    50701 tgagtttttc ttccgcctgg tccagctcgc tgcgcacttc aggtaactgg cgctgtaaga
    50761 attcaaggct ttgtgaatcc tgcgccgcct ggcgagcgat attctgttgc aaatagttgt
    50821 tagcgatgct gttcagaata cgagtaatca actggggatc atcaccagtc atggtaagtt
    50881 ccagcatccc gctttcttta ctgcgttcgc taacggtaaa ggtttcctgc aatgcgttaa
    50941 tcgcttccag ttcggtacgc tggctcagga caaactgtgt tcctggtttg gccttaatgt
    51001 ccgcgatagt cagcgcaacg ccatcttttt ccagacgctg gccgaccata ccattgacgg
    51061 tgaactcttc accttccagt gtatagtggc cgttttcccc aaccgtgagt gtcagttgct
    51121 gatcctgacc attcagttgt ggaatatgca tccagctgat cgccagctca cctggttttt
    51181 ctttggttaa tctcgcccag ccgcgaccca caatcggaaa atacttctgc tcaactatgt
    51241 cgcgcagatt cagttcagca atggttttac cgagaatcat gcgcgattgc agcagttgga
    51301 tctccggtgc agactcgggc gatgagttag ggatcatatc gctcaggccg ctgagaatgg
    51361 cgttgccctg tttttgctca acctggacca gagtatctgc ctgataaatt ggtgtgctta
    51421 acagcgagta agcgacagcg atcagcgtga ataacgcggt cacgctgata ataaacttac
    51481 ggtgatccca taactcgccg accagacgaa gcagatcgat ctcattttcc tgagtgctgc
    51541 ctggtggcgt attcatattt ttagttgtca tcgtatttta taaactcaaa ttatcggctg
    51601 aggcgcttcg cccattcctg actggcgcgc tccaacatac cgtagacatg ttcaaatgcg
    51661 tcctgacttt tacgataggg atccgggatc tctttctgtt ccagccattg cccaaacagc
    51721 attgtcttcc cgcggacctc gggagcaatt gcggtaacct gagcaatatg ttccgactcc
    51781 attgccagaa tcagatcgta atttcgtgcc atctcagcag tgagcttacg tccggcatgt
    51841 ccttcaagag atacgccatg attagcggca acatctgccg ctgtcgcatc tgcagggtgt
    51901 tttatcagac catgaacacc tgccgatttg acttttacgc ccggcagacg tttacgcagc
    51961 aagcgctcgc caatgggcga acggcaaatg ttgccggtac aaaccaccag gattgagtta
    52021 aattttagtt gggccatgtt tgaatccacc gtgaagtctc tgtcaggtca tgcaccccgg
    52081 aaatcgtcgg taccagcaga gaaatcacac ggttccaacg agccagcggc gcggtagtga
    52141 cataaacaat gtcgtaaggt tcaagctgga attccgttcc gagtatcatt gccgatgcgt
    52201 ccttcgcatt cagctgataa atattggcga ttttgccatt ctgcttgttt tgcgttgcac
    52261 gaataacaaa aatccccgtc gcatcagcga catcctgatt cataccctca gcgttaccca
    52321 gcgcttccgc cagggtcata ccgctgcggt ccattttcag cgtgctctgt ttacccactt
    52381 cacccatcac gaagactttc agatcatcgt tgcgcggaat aaataaaata tcgcccgggt
    52441 gcagcagttt gttctggcgc aagtcgccac gctgcatcag cgcatagagg ttcactttgg
    52501 tctttacgcc attctgggtg agcaccacgt tgcgccagtc tgcatctgcg gtcagaccgc
    52561 ccgcggcgtt aatcgcatcc atgatggtca gaggaatatt ggtaatcggc tgctgacccg
    52621 atttgctgac ttcgccggtg acgtacgctt tttgcgatcg gaacgccgcg acgctgacat
    52681 cgacttgcgg gctttcaatg actgaatcga gacgcgctgt aatctcgttg cgtacctggg
    52741 taagtgtttt cccggccact ttcaggcgac caatataggg gtagaaaatc gcaccatcgg
    52801 cgttaaccca gttgccggta tcactggcgc tacggtattg ccctgcgggg gtggttagtt
    52861 ccgggtgatc ccagaccgta accatcaaca catcaccaat cccgatacga tattcatagt
    52921 ttgcgatttg ctgctccagc tccgggttag cctgcgacat aatcggtttg gcgcgcaact
    52981 gatcaatcaa tcctggcgtg acgggataga tattgaccat tttgtccagg tcatactgat
    53041 tgtcaggtag ttcaatgaca tccttgttac tggtcgataa attctgacca ggaacaaggg
    53101 tacatgctgt caaagacaga acagccaatg tcagtaccga aaacttaaaa atgttctttt
    53161 tcattggttt atatttatca gtcaatgtaa tgttatgaat tacgtcaggt tattgtccaa
    53221 ccggtacttc tcgctctgcc gtcattggat agagctggta tttgcgtgac agcatttgtc
    53281 caccatcacg cgtcaatggt gtccacgaaa ccaccgcgcg gttgcggtta ggtccaatgg
    53341 tcatcaagtc gaacggaatc gagatataaa aacctttact aaagccgcct tcgccgtagt
    53401 catctttaga tacgttactg attgccgccc atacccctac cgccacgccg ctgtcaaagc
    53461 gtttggcgac gtcgatcgtt gcccctttat cttttgccag atattgccca acgctaagtt
    53521 tcatcagtac gccattgagc gtcggcgggt tccagtaagc cgtcacgaaa ccagttggcg
    53581 tggaataatc ggtgaagcgc atcatgttgt cccagtcacg ttgcttcacg tagttaacgt
    53641 ccacacccag cgcccagctg gcatctagcg ggcgataaag cagctcggaa ccgacaccgg
    53701 cgtacatcgt ttccagataa ccgccataca cctggccata gaaaccattg cctaagtcgg
    53761 caaagtagtt cgcctgcaag ttgttgagat aaacgtcatt gcgaacgtaa tcacggatat
    53821 gcgtgcgcac gcggggcagg gtagagtccg cgggcaacag cgaagactta aacttgtcgt
    53881 agttgttgta aatattggtg aaaataccgc catcaagcag caggtggtcg gtaaaccagt
    53941 agcgggcact ggacatcagc cccagctgga acatatagaa atcttccggg ccgcccagcg
    54001 actgtgaaag tgttgggttg aaagagtagc taaagcgatc ttcacgaata cggtagcccc
    54061 gaccaaaggc agaaagatct tctgcttcaa cacgttgttg ttgaagttgc tctgattgac
    54121 caggcgctgt accagccagc tgtttgcgca ggctggctac gtcggtttcg gtagtcacca
    54181 tcgccatatg ctcgcgcttt tgcgtcacgc taatcttctc aacgccttgc ggcaggttat
    54241 tcaccagaat ccggttggca cgatcgaccg cttcgcgaga atcacggtat ttgtattgtt
    54301 gaccagacat atacagcgtc ttatcgcgca gctgaatttc cggtgcttca aaacctgcgt
    54361 tgtacttcag cgcggtaagt tggttcgcta ctgtggtgta ctgcaatcct tcagattcag
    54421 gcgcaggttg atatgccggt tttggcgtat cgcgcagggc agggcgcaga tcgttgaaat
    54481 tggtccgtaa cgtgaagcca aacatcaacg tgttaccgcg ttcataactc aggttgagat
    54541 ctgcccagct ggcagcgcga taaactgcgc cgacgttgaa atggcttgcc tgaggcagtt
    54601 tgccagcgaa atcattctgg taattgttgc catcgtattc gagtttcaga cgcaggggat
    54661 tccacggcgt ttgatactca atgccgccaa agatggaagc cgggccacga aagatatcgc
    54721 taaagctgat atcgcccgca tcgtgagact ctgcgcgatg acaatattta tcgctcaccc
    54781 ggcaaaacgg gttggtaata ttgcccgcat taccggcgta gccccatgcc atcccgaggg
    54841 tgaaatcaaa tggccccgct tgcttgctgg ccaccagata ctcaccgtca aacagacccg
    54901 taccagcaat atcacgttta ccaaacgcca cttgcggtag ccagtaacct tcttcccaca
    54961 ggcgaagttt aaaatcgaat gatttgtctt tgtatgactg atcgccgctg aaatcttccc
    55021 actggctata tttgcgtgtg cgcacatccg tataacgaat ggtgccttcc agccatggga
    55081 acagcgccac ggaggtggag tagaaccggt attgatcgtt atcccggtag ttgacgctga
    55141 attcaccttc cggtgcgatg cgcgcatttg gcatctgcaa caatcctgtg ccgccaaagt
    55201 ctgattgcga cgaacccagc ggatccgggt aggttaatac ttcagcatga caggcactgg
    55261 agacggcaat ggccaggcag cttaaaagat aagaattctt cttcatcagt caggaacacg
    55321 ttgcgttaaa acagacacaa tctggtcatt taaatcagca tatttttcgg gcagtacatg
    55381 cgcagaaaag cccaaccaaa gctggctgcc aggcggtggt tccacatgtc ttttattcca
    55441 cagggcaaca ggggcgacga cagtctcacc ctcaggcgta atcaccataa cgttattctt
    55501 gtccgcgcct gcaaggcggg ggtgatcttg cagataatcg gtaacgctac ggcctgcctg
    55561 ccagggcaat tgcccggccc cagaaaccgc acccagcaga gtaatagtca ccggacgctg
    55621 tacggtatag agcgtatagt ccccgaccaa cgggggattg ctgttctcat ccactcgtac
    55681 aaaatcaggg tccagtttga ccggtaagcg accagtgata ttgagattga gtaactgctg
    55741 gcgcacggat ttaatcgttg ccgccacatc gtcgtcagct tctgcttccc aggaagcaag
    55801 ctgtgccatg acatgttggt agtctttaag cgctttagct ttcgccgcag agtcggtcag
    55861 caaggcacca ggccaccaga ggcgatcgcg cagttgcggt tgtgtcacca actggacaac
    55921 attttccatg gggccaacgg aaagtgtctg ttgttcgcca ggcagataaa tagtcaccgt
    55981 tccttgcgca aaggcatggg gtgtcattac gtaaagtacg ctggcaatga aatacgactg
    56041 taatttattc atggtgcggg tttaagaaac gtcatttcta cgggaataac ccctgcgcct
    56101 aacatttgcc gactttgacg aacctggcca gtagccgaat cgacccagaa tgtgttttgc
    56161 cactgttttt ctggccgcgt ggaagtcacc tcttcctgcc agacattaca cagcacattg
    56221 cgacccgcaa tattcagcgt ctctaatcca gcaaaggaaa aagtagaact cagggtcgca
    56281 gagcgaaaat ggttgtcttc actccagaga atatcgcgcg tccagcggct gccatctttt
    56341 attgccagcg cgttacgcag agggtcctgc ccggaatttg tcacttccag cagattatta
    56401 ttaagtttga cggtttttaa aagacgtcca ttgtgggtaa ccagcatggc gttatcctgg
    56461 gataaccatt tgctttgttc ttgttctata tatcccagta ccacaaagat tcgttgccct
    56521 tcattaaggc gtaaatacat cgtggaataa ggcaacgcct ggatctgctg atccgctacg
    56581 gtgatatcct gattatcgaa aaggctggcg cgaaatgtat cgaccatact ttgctggctg
    56641 tgcgtacatc ccgcaagaaa tagtgcgaaa atcgataaaa taagagggcg cacctttaat
    56701 ccttgaaagg ttaattaaaa aaatgcaaat ctctaaagcc cggtacgaat atcgcaaccg
    56761 ggtatcattt aactttaatt tcgacgcgtt aaagcagtat tagcgtgtag tggacgtggt
    56821 ggttgaggtg ttatgagaac cgccatcgcc actgtcattt gctgcaacaa ctgccaacgt
    56881 cgccacacca acagccgttg ccacaccgac aactgtcgcc gtgccgacag cgccagcagc
    56941 cacgccacca gtggtagtag cagtcgtggt tgcgccggta gtggtcgttg tggtgccagt
    57001 agtacccgtc gttgcctcgg tcccgtttgt tgcttcagag gcagcaaacg cagctggcgt
    57061 cgtcaacatg aaggccataa ggattgcaga aagcttgtgt ttcataactt ttcctttatt
    57121 catcgcatgg acaatacgta cgcttgagat caggattgtc tactttgagt ataacatcct
    57181 tgtgaaatct ccctttcctc ataccgcttg cactatatac tgatactgcg ggtaggaaag
    57241 gtgcgcagag ggaaatataa gattattaac gatcccctct ggaaatacga cttgtgagca
    57301 taagataaat cttagcaaaa tacaaattaa agataaggaa attgttacga aagctattaa
    57361 ttattgttgg taatatagtt tcaagtggta ctataactgg ctgttaaatg gcatttataa
    57421 ctattaggtg cagagatatt cgcttaaatg gattagtttc tttgtggaaa agtaactgat
    57481 attataatgt atgatgttga ctattgcact attccaataa aaccagtcag ctttaaacaa
    57541 gcagcgtcat attaagagag ataaacattt gccgctgttg gtcctcgcag gccatttacg
    57601 cggcaaaatt ccacacgtaa tcctggtata agcacttctg cgtcgcgggg agtgaatgcg
    57661 gaaatatgga cctgaacttc tttacgaccg tcggagggga taatgaatcc tttgccgctt
    57721 ttgcgatcaa aggttttgac aattcctgtc attttacggg acaaacaaat tccttactga
    57781 aaatactgcg ctgcactata cggggttaat aaaataaagc cagcgatatt taagaccgcc
    57841 g
//
LOCUS       DAJTYZ010000010        57431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000010 DAJTYZ010000000
VERSION     DAJTYZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.010"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            146..1489
                     /gene="argG"
                     /locus_tag="OO596_000598"
     CDS             146..1489
                     /gene="argG"
                     /locus_tag="OO596_000598"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6716547.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1497..3104)
                     /locus_tag="OO596_000599"
     CDS             complement(1497..3104)
                     /locus_tag="OO596_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6716548.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3582..3668)
                     /locus_tag="OO596_000600"
     tRNA            complement(3582..3668)
                     /locus_tag="OO596_000600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3632..3634),aa:Leu,seq:gag)
     gene            complement(3683..4015)
                     /gene="secG"
                     /locus_tag="OO596_000601"
     CDS             complement(3683..4015)
                     /gene="secG"
                     /locus_tag="OO596_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6716549.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="OO596_000602"
     CDS             complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="OO596_000602"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6716550.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5573..6421)
                     /gene="folP"
                     /locus_tag="OO596_000603"
     CDS             complement(5573..6421)
                     /gene="folP"
                     /locus_tag="OO596_000603"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6716551.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="OO596_000604"
     CDS             complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="OO596_000604"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6716552.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="OO596_000605"
     CDS             complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="OO596_000605"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6716553.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9300..9593
                     /gene="yhbY"
                     /locus_tag="OO596_000606"
     CDS             9300..9593
                     /gene="yhbY"
                     /locus_tag="OO596_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6716554.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9749..10225)
                     /gene="greA"
                     /locus_tag="OO596_000607"
     CDS             complement(9749..10225)
                     /gene="greA"
                     /locus_tag="OO596_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6716555.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10473..11906
                     /gene="dacB"
                     /locus_tag="OO596_000608"
     CDS             10473..11906
                     /gene="dacB"
                     /locus_tag="OO596_000608"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6716556.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="OO596_000609"
     CDS             complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="OO596_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6716557.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13280..14245)
                     /locus_tag="OO596_000610"
     CDS             complement(13280..14245)
                     /locus_tag="OO596_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6716558.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="OO596_000611"
     CDS             complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="OO596_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6716559.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="OO596_000612"
     CDS             complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="OO596_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6716560.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15220..16191
                     /gene="ispB"
                     /locus_tag="OO596_000613"
     CDS             15220..16191
                     /gene="ispB"
                     /locus_tag="OO596_000613"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6716561.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16420..16698
                     /gene="sfsB"
                     /locus_tag="OO596_000614"
     CDS             16420..16698
                     /gene="sfsB"
                     /locus_tag="OO596_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6716562.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16746..18005)
                     /gene="murA"
                     /locus_tag="OO596_000615"
     CDS             complement(16746..18005)
                     /gene="murA"
                     /locus_tag="OO596_000615"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6716563.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18060..18314)
                     /gene="ibaG"
                     /locus_tag="OO596_000616"
     CDS             complement(18060..18314)
                     /gene="ibaG"
                     /locus_tag="OO596_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6716564.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18474..18767)
                     /gene="mlaB"
                     /locus_tag="OO596_000617"
     CDS             complement(18474..18767)
                     /gene="mlaB"
                     /locus_tag="OO596_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6716565.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18767..19402)
                     /gene="mlaC"
                     /locus_tag="OO596_000618"
     CDS             complement(18767..19402)
                     /gene="mlaC"
                     /locus_tag="OO596_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6716566.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19421..19972)
                     /gene="mlaD"
                     /locus_tag="OO596_000619"
     CDS             complement(19421..19972)
                     /gene="mlaD"
                     /locus_tag="OO596_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6716567.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19977..20759)
                     /gene="mlaE"
                     /locus_tag="OO596_000620"
     CDS             complement(19977..20759)
                     /gene="mlaE"
                     /locus_tag="OO596_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6716568.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20767..21576)
                     /gene="mlaF"
                     /locus_tag="OO596_000621"
     CDS             complement(20767..21576)
                     /gene="mlaF"
                     /locus_tag="OO596_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6716569.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21786..22763
                     /locus_tag="OO596_000622"
     CDS             21786..22763
                     /locus_tag="OO596_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6716570.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22777..23763
                     /gene="kdsD"
                     /locus_tag="OO596_000623"
     CDS             22777..23763
                     /gene="kdsD"
                     /locus_tag="OO596_000623"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6716571.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23784..24350
                     /gene="kdsC"
                     /locus_tag="OO596_000624"
     CDS             23784..24350
                     /gene="kdsC"
                     /locus_tag="OO596_000624"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6716572.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24347..24922
                     /gene="lptC"
                     /locus_tag="OO596_000625"
     CDS             24347..24922
                     /gene="lptC"
                     /locus_tag="OO596_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6716573.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24891..25448
                     /gene="lptA"
                     /locus_tag="OO596_000626"
     CDS             24891..25448
                     /gene="lptA"
                     /locus_tag="OO596_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6716574.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25455..26180
                     /gene="lptB"
                     /locus_tag="OO596_000627"
     CDS             25455..26180
                     /gene="lptB"
                     /locus_tag="OO596_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6716575.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26228..27661
                     /gene="rpoN"
                     /locus_tag="OO596_000628"
     CDS             26228..27661
                     /gene="rpoN"
                     /locus_tag="OO596_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6716576.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27684..27971
                     /gene="hpf"
                     /locus_tag="OO596_000629"
     CDS             27684..27971
                     /gene="hpf"
                     /locus_tag="OO596_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6716577.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28089..28580
                     /gene="ptsN"
                     /locus_tag="OO596_000630"
     CDS             28089..28580
                     /gene="ptsN"
                     /locus_tag="OO596_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6716578.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28626..29480
                     /gene="rapZ"
                     /locus_tag="OO596_000631"
     CDS             28626..29480
                     /gene="rapZ"
                     /locus_tag="OO596_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6716579.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29477..29749
                     /gene="npr"
                     /locus_tag="OO596_000632"
     CDS             29477..29749
                     /gene="npr"
                     /locus_tag="OO596_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6716580.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29963..30595
                     /gene="yrbL"
                     /locus_tag="OO596_000633"
     CDS             29963..30595
                     /gene="yrbL"
                     /locus_tag="OO596_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6716581.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30592..31320)
                     /gene="mtgA"
                     /locus_tag="OO596_000634"
     CDS             complement(30592..31320)
                     /gene="mtgA"
                     /locus_tag="OO596_000634"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR6716582.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31317..31970)
                     /gene="elbB"
                     /locus_tag="OO596_000635"
     CDS             complement(31317..31970)
                     /gene="elbB"
                     /locus_tag="OO596_000635"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6716583.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32200..34536)
                     /gene="arcB"
                     /locus_tag="OO596_000636"
     CDS             complement(32200..34536)
                     /gene="arcB"
                     /locus_tag="OO596_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6716584.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34632..35561)
                     /locus_tag="OO596_000637"
     CDS             complement(34632..35561)
                     /locus_tag="OO596_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6716585.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36236..40696
                     /gene="gltB"
                     /locus_tag="OO596_000638"
     CDS             36236..40696
                     /gene="gltB"
                     /locus_tag="OO596_000638"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6716586.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40709..42127
                     /gene="gltD"
                     /locus_tag="OO596_000639"
     CDS             40709..42127
                     /gene="gltD"
                     /locus_tag="OO596_000639"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6716587.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42311..42790
                     /locus_tag="OO596_000640"
     CDS             42311..42790
                     /locus_tag="OO596_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6716588.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42807..43112
                     /locus_tag="OO596_000641"
     CDS             42807..43112
                     /locus_tag="OO596_000641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6716589.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43188..43652)
                     /locus_tag="OO596_000642"
     CDS             complement(43188..43652)
                     /locus_tag="OO596_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6716590.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43649..44524)
                     /gene="nanK"
                     /locus_tag="OO596_000643"
     CDS             complement(43649..44524)
                     /gene="nanK"
                     /locus_tag="OO596_000643"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6716591.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44521..45210)
                     /locus_tag="OO596_000644"
     CDS             complement(44521..45210)
                     /locus_tag="OO596_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6716592.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45258..46748)
                     /gene="nanT"
                     /locus_tag="OO596_000645"
     CDS             complement(45258..46748)
                     /gene="nanT"
                     /locus_tag="OO596_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6716593.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46858..47751)
                     /gene="nanA"
                     /locus_tag="OO596_000646"
     CDS             complement(46858..47751)
                     /gene="nanA"
                     /locus_tag="OO596_000646"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6716594.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47873..48664)
                     /gene="nanR"
                     /locus_tag="OO596_000647"
     CDS             complement(47873..48664)
                     /gene="nanR"
                     /locus_tag="OO596_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6716595.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49044..50366
                     /gene="dcuC"
                     /locus_tag="OO596_000648"
     CDS             49044..50366
                     /gene="dcuC"
                     /locus_tag="OO596_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6716596.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50409..50906)
                     /gene="sspB"
                     /locus_tag="OO596_000649"
     CDS             complement(50409..50906)
                     /gene="sspB"
                     /locus_tag="OO596_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6716597.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50912..51550)
                     /gene="sspA"
                     /locus_tag="OO596_000650"
     CDS             complement(50912..51550)
                     /gene="sspA"
                     /locus_tag="OO596_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6716598.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51945..52337)
                     /gene="rpsI"
                     /locus_tag="OO596_000651"
     CDS             complement(51945..52337)
                     /gene="rpsI"
                     /locus_tag="OO596_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6716599.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52353..52781)
                     /gene="rplM"
                     /locus_tag="OO596_000652"
     CDS             complement(52353..52781)
                     /gene="rplM"
                     /locus_tag="OO596_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6716600.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(53000..54127)
                     /gene="zapE"
                     /locus_tag="OO596_000653"
     CDS             complement(53000..54127)
                     /gene="zapE"
                     /locus_tag="OO596_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6716601.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54321..54719
                     /locus_tag="OO596_000654"
     CDS             54321..54719
                     /locus_tag="OO596_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6716602.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54873..56240
                     /gene="degQ"
                     /locus_tag="OO596_000655"
     CDS             54873..56240
                     /gene="degQ"
                     /locus_tag="OO596_000655"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR6716603.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56330..57397
                     /gene="degS"
                     /locus_tag="OO596_000656"
     CDS             56330..57397
                     /gene="degS"
                     /locus_tag="OO596_000656"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6716604.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14264 a        15303 c        14255 g        13609 t
ORIGIN      
        1 agcgcattat tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa
       61 atgaaaactc atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc
      121 agttaattaa gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca
      181 acgtattggt atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg
      241 acaaaaggga gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga
      301 ttatgatgcg atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga
      361 ctgccgcaaa caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa
      421 caccaccggc ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac
      481 catgctggtt gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta
      541 taaaggaaac gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca
      601 gatttacaaa ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat
      661 gtctgaattt atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc
      721 cacagactcc aacatgcttg gtgcaacgca cgaagcgaag gatctggaat acctcaactc
      781 cagcgtcaaa atcgtcaacc caattatggg cgtgaaattc tgggatgaga gcgtgaagat
      841 cccggcagaa gaagtcacag tacgctttga gcaaggtcat ccggtagcgc tgaacggtaa
      901 aacctttagc gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg
      961 cctgggcatg agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta
     1021 cgaagctccg gggatggcac tgctacatat cgcttatgaa cgtctgttga ccggtattca
     1081 caacgaagac accattgagc agtatcatgc acatggtcgt cagttgggcc gtctgctgta
     1141 ccaggggcgt tggtttgatt cccaggcgct gatgctgcgt gactctctgc aacgctgggt
     1201 tgccagccag atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat
     1261 cctgaatacc gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg
     1321 cgactcggtg ttctcgccag atgatcgtat tggtcaattg accatgcgta acctggatat
     1381 cactgatacc cgagagaaac tttttggtta tgccaaaact ggcctgctct cctcctctgc
     1441 cacttcaggc gtgccgcagg tggagaatct ggaaaacaaa ggccagtaat tcgacatcaa
     1501 ccctgccctt caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc
     1561 acataatcga aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca
     1621 atcacttcct ccagcgtttt cggttgttcc ggctttgtta cccccatcca cgcattaata
     1681 aggtaattat tgtaagctgt cgaaaagatg ttgttttccg ttgtacgatc cacgccatcc
     1741 ccaagaacgg ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc
     1801 ctgcctccat gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca
     1861 aaatacatga ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta
     1921 tctgtgtaat gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg
     1981 gcgctggatt gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca
     2041 atcagtttct tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat
     2101 tcatcaaatc ctctgacata aactgtttcc atggcgcgca tggcgatact ggtaactgct
     2161 gtaccattct gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga
     2221 ttagccatat taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca
     2281 gcagttaaag aaaggggaac cgacagcgca gtgtaaggtg cgccgcttat tgattgatta
     2341 aacagtttga tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca
     2401 tacaaagaca tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata
     2461 cctgtatccc tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt
     2521 tgatgctctt tcgctgctaa agcaaaataa ttgagattga aaaacggcgt ataggtagca
     2581 aatcgcgacg ctagtatata tggactgaat gcttttttat tttttgcatc tttataagca
     2641 aattgacaag cagaaaataa actgcccgaa atgacaatca gcaataatat tcctgtcact
     2701 ttttttgtcg gcaaggaaac atcatatttt attattactg ccaaaaaaag aagggataaa
     2761 aaagcaaaag cacatagata agggatatac atccccagca ttttgacaac ttcatccgga
     2821 tcactctgca gcacactaat cgcaaatcca tcattgaaag ttgtaccaaa ggtacaccat
     2881 gaatagaggc taatactcat gtctgccgca gtcagtaaca caaatggtgc ggcaacaagc
     2941 aggcgaagcg gaagggatga tatacgtttt acaaccagga tggtcaggac gaagaacagc
     3001 aatcgctgaa ccatatgcgc gccggaagag gcgactaagt tcgtaatacc aaaaacaata
     3061 acacaaagat acaacaacca atgagattta aaatcttcta gcaaatttgt tgaatactgt
     3121 catccagaaa tccaagttca caaccaatat aaacaattat caacaatgtt caattttata
     3181 tcagacatgt ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt
     3241 taaatgatgc ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac
     3301 attacgcaga acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa
     3361 gtaaacatat ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc
     3421 agcagaaggg aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct
     3481 acgagtaaag caactggacg agatacagat acctgacaac cattcctcag accaggacca
     3541 aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact
     3601 tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc
     3661 acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag
     3721 ctggctgagt ttgttcggtt ttcgccggtg cactcagatt ttcccattcg ctacctttat
     3781 tggttttgtt gctattgatg ttacccagca ccagactgat gataaagaat aacgttgcca
     3841 gcagcgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg
     3901 aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca
     3961 gaccaacaag gccaattgcc acaataagga aaactaccaa aagagcttcg tacataatca
     4021 acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta
     4081 atctttccca ctgaagtggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa
     4141 ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa
     4201 aaaggcgaca atcgccgcct ttttagccag ttatttaccg ttttaaacgg cttttactgc
     4261 atcggcgata cggtgtgcaa attcagttac ctgcgcttcg tcttcacctt ctaccatcac
     4321 gcgaattaac ggttcggtgc cggatttacg caacagcaca cgtccacggc tacccagcgc
     4381 cgcttcaact tcggcggtca cagctttaac tgactcatgc tcaagcggat cgccgctacc
     4441 tgcggtgtaa cgcacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc
     4501 gtgcaggctc atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc
     4561 gtcaccggta gtggttttat ccagcaggat cacatgaccg gaattctctg caccgatacg
     4621 ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa
     4681 tggaattcct aactgtttca acgccagctc aagccccatg ttactcatca atgtacccac
     4741 agcaccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg
     4801 atcaccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc
     4861 gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc
     4921 ggtagccccc acttcggcat tgatgtttac accgttcggc tcacaaccga tagcgataac
     4981 gttcgccccc agttcacgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcgca
     5041 atccaccaca atcttcagtt cgctgaggct aagttcgttc gggaacgtgg ctttgcaaaa
     5101 ctcgatatag cgacccgcag catcaacgat acggctggct ttacccagtt ctgccgaatc
     5161 aacacagctg atctcttttt ccatttccgc ttcgatggcc tcttctaccg catccggtag
     5221 tttggtgccg tcgatagaga aaaatttaat gccattatcg tagaacgggt tatgcgatgc
     5281 agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt
     5341 tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc
     5401 tgactccagc atatagccag aaatacgcgt gtctttacca ataatgatct tacgggagcc
     5461 gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat
     5521 cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat
     5581 agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt
     5641 ctttgacgtc atgaacacga ataatgtgcg cgccttgcat tgcggcaatg accgcacagg
     5701 ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt
     5761 ttcgtgacat acctaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca
     5821 gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt
     5881 tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca
     5941 cttctgcaaa gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca
     6001 tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg
     6061 aaagggagcg gatatcgtta ataatgtgag cgccaacttt cgctgactca cggatgactt
     6121 ctggtttgga tgtatctacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca
     6181 caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg
     6241 actcgccacc aacgtcaatg atcgtcgcgc cagcgttgat catcagattc gcatgtttca
     6301 ccgcatctat cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga
     6361 ggatccccat tacgtgagga tggctaaggt ccagtgaagt gccctgggca aagagtttca
     6421 tggtgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc
     6481 ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt
     6541 gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt
     6601 gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg
     6661 acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc
     6721 tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt
     6781 acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg
     6841 tttcgctttc gctacgctac ggccgaggaa cacttcacct tcttcttccg cgtacagcag
     6901 tggacccaat ttctcagaga agccccactg agtcaccatg ttacgcgcca ggttggtcgc
     6961 aactttaata tcgttggacg caccggtaga aacatgttcc ggcccgtaga tgatctcttc
     7021 tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct
     7081 gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat
     7141 cgtcactttg tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc
     7201 ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc
     7261 acccatcatg attttatctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt
     7321 gccacgagca gcgaacagtg ccgcttcgtt taccaggttc gccaggtcag caccggagaa
     7381 accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg
     7441 catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccaa ccacaacctg
     7501 acggtcgaaa cggccaggac gcagcagggc cgggtcaaga acgtccggac ggttagtcgc
     7561 ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt
     7621 cagagtctgt tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac
     7681 ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat
     7741 gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat
     7801 agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg ttttaccggt
     7861 acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg
     7921 gctcggctcg cgcagatatt caaccagttc agcaacttct tcttttgctt cgtcgcagcc
     7981 cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt
     8041 accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca
     8101 cacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg
     8161 ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt
     8221 cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat
     8281 ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt
     8341 agagtaatcc accttacggc cattagactc gctgggccca aagctctgga atactgacat
     8401 cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt
     8461 aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc
     8521 aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc
     8581 ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg
     8641 aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc
     8701 acctggcgct aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg
     8761 ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg
     8821 gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc
     8881 gccctgaaga aagtctacac caacgatagg atccataggt aaaagatcgc aagcgatgat
     8941 gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc
     9001 aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag
     9061 tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct
     9121 aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact
     9181 ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga
     9241 tatatgggag atggcggtag aatgacccat tttcaatccc aacgtaagca aaaaatacga
     9301 tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag
     9361 ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt
     9421 tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa acaaaaacct
     9481 tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa
     9541 cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc
     9601 ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa
     9661 tgccacgctc tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata
     9721 catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
     9781 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
     9841 cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
     9901 caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac
     9961 caaaaataac gcgcccattg ttaggcattt tggtgacatc aatcacctgc gcgtttgaca
    10021 gcttggcttc gatgtctttg atacggcctt cgcagaagcc ctgctgttca cgagctgcgt
    10081 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
    10141 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
    10201 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
    10261 tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
    10321 aaaaaatacc gacccaggta caagtcccag gtcagctaca attcacattt tgatagtcat
    10381 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
    10441 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
    10501 tgaccagctg tatagcgttc agtgttcagg ccgcaaatgt tgatgagtac attactcaac
    10561 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
    10621 ttgattacca cagtcagcag atggcgctgc ctgccagtac ccagaaagtg attactgcgc
    10681 tggcggcgtt gattcaactc ggccccgatt ttcgttttac cacgacgctt gaaaccaaag
    10741 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
    10801 cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc
    10861 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
    10921 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgcc gccatagttg
    10981 accgcaactg tttctccgtc tcgctctaca gtgcccaaaa acccggtgat atggccttta
    11041 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgtg
    11101 gttctgccga agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta
    11161 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
    11221 atggagccag ttatgccggt gcaattctga aagatgagtt aaaacaggcg ggtatcacct
    11281 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
    11341 aacagtcggc cccgctgcac gatctgctta agattatgct gaaaaagtcg gataacatga
    11401 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtacct ggtacatggc
    11461 gggccgggtc ggacgctgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
    11521 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
    11581 tgatgcaggt gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
    11641 tgccgctggc gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
    11701 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
    11761 tcattaccac agcgagcggg caacgaatgg cgtttgtgca atatctttct ggctatgcag
    11821 tagaacctgc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
    11881 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggcttc
    11941 agattattga caaagtgcgc tttgtttatg ccggatgcgg cgtaaacgcc ttatccggcc
    12001 tacaaaatcg tgcaaattca acatattgca attctcttgt aggcctgata agcgtagcgc
    12061 atcaggctga tttggcgttt atcatcagtg attaacgctt gtaaatgaac tcaacgcctt
    12121 cttcgtcgtc ttcgtcccag tcgtcatccc agtcttcatc atcctcttca gcaatctctt
    12181 caagctgctg gcgatgataa tcgtcccaca tgaattcgac tttctctggc tgtttcgctt
    12241 cttcagcctg cacgaccggg ttttcaagga taaaggtcat cacatcccag cagagatctt
    12301 tcacgcccag tccgctcgcc gcagagatca gataatattt atcttcccag cccagcgctt
    12361 cagcaatcgc tttcgctttc tcttcagctt ctgccttatc cagcagatcg atcttattga
    12421 acactaacca acgcggtttc gccgccagat cctggctgta tttttccagc tcgctgataa
    12481 taatacgcgc gttttcaacc ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca
    12541 acaggacgcg gcaacgttcc aggtgcttca ggaagcggat acccagacct gcgccttccg
    12601 cagcgccttc aatcagtcct ggaatatcgg caacaacgaa gctcttttcg ttgtccattc
    12661 gtaccacacc cagacttggc accagagtgg taaacggata atccgccact ttcggtttag
    12721 ccgccgatac cgcgcgaata aaggtcgatt tacccgcgtt tggcatcccc aacataccga
    12781 cgtcagccag cagcatcagc tccagcagca gctcacgctt atcacccggc gtaccgttgg
    12841 ttttctgccg tggtgtacgg ttaacggacg atttgaaacg ggtattgccc agaccgtgcc
    12901 agccgccctt agcaaccagc agacgctgac cgtgtttagt catatcgccc atggtttcac
    12961 cagtaccctg gtcgattaca cgcgtaccta ccggcacttt aatcgtcacg tctttaccgc
    13021 gcttaccggt acaatcgcgg cttgcgccgt tctgaccgcg ctctgcacgg aaagattttt
    13081 caaaacgata atcgataagc gtgttcaggt tttcgtcggc ttccatccat acgtcaccac
    13141 catcaccacc gtcgccgcca tccgggccgc ctttcggaat atacttttcg cggcggaagc
    13201 tcacgcaacc attaccgcca tcacctgcaa cgaccagaat cgatgcttca tcaacaaact
    13261 tcattttatt ctccataaat cattcgcctg cgcggggttg cgataccacc gttgtatgct
    13321 tacgtaatcc gccccaaata cgatgaccaa tggcggaata catcgcgccc gcaaccacga
    13381 caaacgcacc gagataacct aaaaggttta acatcggtct ggcgaagaaa tcgggccagg
    13441 ccagtgataa aagatctgaa aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc
    13501 tcacctgcgc tgcctgccag cgagccatcg cttccgccag ggcgccatat cctaccaatg
    13561 tattcagtcc gcaaaaaatt aaacatgcga gctgccagtg gctaagctgc gctatcactc
    13621 caggctttgc cagagggaag agcgcaattg tacataaagt gtacagtaaa aacaggatct
    13681 gcggtgaggc cagccgacgc aataaaacct tttgcgccac gccataactc acccaaaccg
    13741 tcgccgcacc gaccccaaag ataactcccc aggtgtaatc ggtgagcttt gtaaatatct
    13801 cgaccagact ggtgttaaaa aacatcacca ggccgctcag gagcatcaat gcccctacaa
    13861 cctgagtgct gcgcattttc tctttcagga taaatacgct ggcaaccatc atgccaactg
    13921 gcgagagttg cccaatcacc tgcgaagcgg tcggactcag gtattgcaag gatgagctga
    13981 acaggatgaa gttcccaaac agcccggcgg tcgccactgc caacaaaatc aaccagcgtg
    14041 gcttacgaaa cacgcgtaat ggcggcaacc tcttcttcac cgcaagaatg gcaccaaggc
    14101 caatactcgc catcaagaaa cggtaaaaca cgattgtcgg aggttccatc acctccagca
    14161 cctgcttcat tgcgattggc aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa
    14221 tgccaatgcc tgcctgctgc ttcataccgt attccctgca aagcgcattt gcggattaac
    14281 gtcgcaccat gcgcattttt accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg
    14341 ggctttcatc cgttaccggg acgcgaaaaa cttattcagc ttcgatgctg ataaatttac
    14401 ggtttttcgg gcctttaact tcgaatttca ctttaccgtc tgctttagca aacagagtgt
    14461 ggtcacgacc gcaacctacg ttagcgccag cgtggaattt ggtaccacgt tgacgaacga
    14521 tgatgctacc cgccagaacg gattcgccac cgaaacgctt aacgcccagg cgtttagctt
    14581 ctgaatcgcg accgttacgt gtggagccgc cagccttttt atgtgccatt tgaaatctct
    14641 cctcaggtct taggcgctga tgccagtaat tttcacatca gtgaaccact gacgatgacc
    14701 ctgctgctta cgatagtgtt tacgacgacg aaacttaacg attttaactt tctcgccacg
    14761 accgtgagca acaacttcag ctttgattac gccgccatca acgaaaggaa cgccgatttt
    14821 gacttcttca ccgtttgcga tcatcagcac ttcagcgaac tcaacagttt cgccagttgc
    14881 gatgtccagc ttttccaggc gaacggtctg accttcgctt actcggtgtt gtttaccacc
    14941 actttggaaa accgcgtaca tataaaactc cgcttccgcg cacacctttt caatgattca
    15001 gagtgcgcta taaatattca caatagggcg cgaatattac gcaaaacgca cgcctttgac
    15061 aagtgctaca gtcaatacac gaagaaaaaa aacacaactt gtacggtaac gtttatctgt
    15121 gccatttttt cagtacaatc accctatatt cctaaccata aaccctaagt tgcctttgtt
    15181 cacagtaagg taatcggggc gaaaagcccg gcttttgcga tgaatttaga aaaaatcaat
    15241 gagttaaccg cgcaagatat ggcgggtgtt aatgcggcaa tccttgagca gcttaattcc
    15301 gacgtccaac tgatcaatca gttaggctat tacatcgtca gcggcggcgg taaacgtatt
    15361 cgtccgatga ttgctgtact ggctgcacga gctgttggct atgagggaaa tgcgcatgtc
    15421 accattgctg ccctgatcga gtttatccac acggcgactc tgctacacga cgacgttgtg
    15481 gatgaatcag atatgcgcag gggtaaagct accgccaacg ccgcatttgg caatgccgcc
    15541 agcgtgctgg taggcgattt tatttatacc cgcgcgttcc agatgatgac cagcctcggt
    15601 tcactcaaag tgctggaagt catgtcagaa gctgtaaacg tcatcgcaga aggtgaagtt
    15661 ctgcaactga tgaacgttaa cgatccggac atcactgaag aaaactacat gcgcgttatc
    15721 tatagcaaaa ccgcgcgtct gtttgaggct gccgcgcagt gttccgggat tctggctggc
    15781 tgtacgccgg aggaggagaa aggcctgcag gattatgggc gctatctcgg cactgctttc
    15841 cagttgatcg acgatttact cgattacaat gccgatggcg aacagttagg taaaaatgtc
    15901 ggcgacgatc tgaacgaagg taaaccgacg ctgccgctgc tgcatgcgat gcatcatggc
    15961 acaccagaac aggcacagat gatccgtacc gccatcgaac agggtaacgg tcgccatctt
    16021 ctggaaccgg ttctggaagc aatgaacgct tgtggatctc ttgaatggac gcgtcagcgt
    16081 gccgaggaag aagcagacaa agccatcgca gcattacagg tgctcccgga caccccttgg
    16141 cgagaagcac tcatcggcct cgcgcacatc gctgttcaac gcgatcgtta atcccctccc
    16201 ttcatcccgc gcagtgcgcg ggatgattcc agtaaattcc ataaaaacac taattcagcg
    16261 ctataacacc tgaaaaaaaa gggcgatgaa tctttatata ttctgaatat tcacactctt
    16321 tacaggaact ttttagagca ataggccatc aggagtatag tgatgcccga cagaagaagt
    16381 gttctgaatg aaagcgacta acttaaggag tgaggaaaga tggaaagtaa tttcattgac
    16441 tggcatcccg ctgacatcat tgcgggtttg cgcaagaagg gaacgtcaat ggcggcggag
    16501 tctcgcagaa atggtttgag ttcctcaacg ctggcaaacg cattatcgcg cccatggccg
    16561 aaaggagaga tgattattgc gaaagccctg ggaactgacc cctgggttat ctggccatca
    16621 cgctaccatg atccgcagac ccatgagttt atcgacagaa cgcagttgat gcgtagctac
    16681 actaaaccga aaaaatgagt ggtctggcgg tagccccgcg aacggggctg ccagctctca
    16741 gacgattact cgcctttcac acgctcaata tttgcaccta aagcgcgcag tttgtcttca
    16801 atgcgttcgt agccacgatc gatgtgataa atacgatcaa ccaccgtcgt cccttccgca
    16861 atacagccag ccagcaccag gcttgctgat gcacgcagat cggttgccat aacctgtgcg
    16921 ccagaaagtt tttcaacacc gtgacaaata acggtattgc tttcgatttc ggcatgcgcg
    16981 cccatacggc taagctccgg cacatgcata aagcggtttt caaagaccgt ttcggtgatg
    17041 aatccggtcc cttctgccac caggttcaac agcgtgaact gggcctgcat atcggtcggg
    17101 aatgccggat gcggcgcggt acgtacgtta acagccttcg gacgtttgcc atgcatatcc
    17161 aggctaatcc agtcttcgcc gacttcgatg tccgctccag cgtcacgcag tttcgccagc
    17221 acggcgtcga gagtatctgg ctgcgcgtta cggcagataa ttttgccgcg agaaatcgcc
    17281 gccgccacca ggaaagtacc ggtttcgata cgatccggta gcacgcgata gacaccgccg
    17341 cctaaacgtt ccacaccttc gatgacgata cgatcggtgc cctgaccgct aattttcgca
    17401 cccagcgtaa tcaggaagtt cgcggtatcg acgatttccg gttcacgcgc tgcattttca
    17461 ataatcgtgg taccttccgc cagggttgca gcacacatga tggtcaccgt tgcgccaacg
    17521 ctgactttat ccatcacgat atgtgcgcct ttcagacgac catcgacgga agctttaacg
    17581 taaccttctt ccagtttgat ggtcgcgcct aattgttcga ggccagaaat gtgtagatca
    17641 accggacgcg caccgatcgt acaaccgcca ggcagtgaaa cttgcccctg accaaagcgc
    17701 gctaccagcg gccccagtgc ccagatagaa gcacgcatgg ttttaaccag atcgtaaggt
    17761 gcgcagaata cattaacgtc gcgggcatca atatgcacag aaccattacg ttctactttc
    17821 gcacccagct ggcttagcag cttcattgat gtatcgacgt ctttcagttt tgggacgttc
    17881 tggatctcta ccggttcttc cgccagtaga gcggcaaaaa ggataggcag agcagcattt
    17941 ttagcgccgg aaattgtgac ttcgccctgg agcttcgttg gcccctgaac acgaaattta
    18001 tccatttagt ttgttctcag ttaacaattc atatccgcta ccggcgaatc gcccatagct
    18061 caaaagccgt tcagtttgcg atcgcgcgcc cactccgcag gggtatacgc tttgatcgac
    18121 acagcatgaa tgcggttatc cgcaatatat tccatcagcg ggccatagac cgtctgctgt
    18181 tttttaaccc gactcatgcc gtcaaacaac tcacccacgg caataacctg aaagtggctg
    18241 ccatcgccgg aaacgtggac ttcctggagg gagagagcgt tcatcaacac gctctgaatt
    18301 tcattatttt ccatgggatc ttcaatcatc agttaataaa ccagcgaaac atcttagagc
    18361 aaagttgcgc tggcataaat aagcaaaaag cctcgctgat aaatcagaca aggctcgact
    18421 tgcaggcagg tttgccggac aggcggttaa cgccatatcc ggcctgaaaa aatttaacga
    18481 ggcagaacat cagcaggcaa attatacaat ttcgcgaggg tatacacttt gtcgtttacc
    18541 ccctgaagcg tcacgttgtt gccctgcttt ttcgccagat cgataagatg gagcagcagt
    18601 gccagtccac ccgtatccac gcgggacaca cggctaagat cgatgcaggt aatccccttc
    18661 accgcttcct cacgcatttc ccaaagcggt agcagaacgt cctgatccag ctctccggat
    18721 aacgccagcg tgtcacccgt ctgcatccag ctcagtgact cgctcattat tttttctctt
    18781 ccagagtgat tttctgttga gaaatcgatt tcagttgcgc agtcaggccg tcgatacctt
    18841 tggtacgcag cagcgttccc cactcgtttt gtttggtggt gatcatactg acgccttcag
    18901 caatcatgtc gtaagcctgc cagtttcccg tctgggagtt tttacgccac tggaagtcca
    18961 gacgcaccgg cggacggcca ttcgggtcaa taatggtaac gcgaataggc acaatggttt
    19021 tatcgcccag cggctgttct ggcgcaatct gataggtttg accgtgatac atcgccagcg
    19081 cctgaccgta agcctgcttc aggtactcac ggaaagcggc aaagtaggct tcacgttgag
    19141 caggggtcgc actcttgtaa tactggccca gcaccagcgc accggcgtat ttcacctgta
    19201 cgtatggcag cagttcctga tcaacaatgg tacgcagata atccgggttg gcccgaattt
    19261 gcggttgctc attcttcagg cgatcgaacg ttttctgcgc cgcctcgtcc atcagcttat
    19321 aaggattggt ctggtctgcc gcggttgccg cactcagagg tgcaatcacc agcaaagcga
    19381 ccatcattaa acgtttaaac atgcgtcggt tctcctgaaa ttatttcgtt gtacccacag
    19441 gttcagtggt ttcattatta cctggcgcag cagctggcgc atcgccacta ttcttattgt
    19501 catcgccttt actaccgtaa aggaactgac caatgagatc ttccagcacc atcgcagact
    19561 tagtgtcctg aattgtatcg ccatccttca ggatagcagt ccccagttcc gggtcttcaa
    19621 aaccgacgtt taatgccaga tattgttccc ccagcaggcc ggaagtacga atgctcagcg
    19681 aactggtatc tggaatgtgg ttataacgtt gttcaatttc cagcgttacg cgcggcagat
    19741 aggttttcgg gtccagcgta atatccgcca cccgacccac aacaacgcca ccaatactga
    19801 ccggagagcg cgctttcagg ccgccaatgt tatcgaacgt cgcataaagc gtgtaggtcg
    19861 gttcagtacg tatggacgtc acgttcgccg ccttcaggca aacaaacagc gccgccagca
    19921 gtgctgctaa taaaaagata cccacccaaa tttcattttt tttcgtttgc atgaactcaa
    19981 ttcccaaaca tcaatgcggt cagcacaaaa tccagcccca gaacagccag agacgagtgg
    20041 acaacggtgc gagtggttgc ccggctaatc ccggcagacg tcgggatggt gtcgtagccg
    20101 ttaaacaacg aaatccacgt caccgtgatg gcgaacacca cgctcttaat cagacagttg
    20161 accagatcca tacgccagtc gacggcattt tgcattgccg accagaagaa cccgctatca
    20221 atgcctttcc aactgacgcc gaccagagat ccgccccaga tccccacggc gacgaaaata
    20281 accgtcaaca gtggtaatga aataacccca gcccagaaac ggggagaaat aacccgacgc
    20341 agcggatcca ccgccatcat ctccatactg gagagttgct ctgtagcgcg catcaggccg
    20401 atttctgcgg ttagcgccga accagcacgc ccggcaaaca acaacgcggc aaccaccggc
    20461 cccagttcac gcagtagcga taacgccacc agcataccca gactggtttc cgcactataa
    20521 gtggtcagaa ccagataacc ttgcagcccc aacaccattc cgatgaacac gccagaaacc
    20581 acaataatca gcatcgacag gacgccgaca ttatagagct ggcgcaccag cagcggcgca
    20641 tgtttgcgaa actccggttt gccgaccagc gcattgaata acattaaccc ggcccgcccg
    20701 aacgttctca gggttttaat ccctttatgt ccgagcgacg ccagcgcatt taacagcatg
    20761 agtggcttaa ctccctggta aaagatcagc gtgataatcg ccggcaggat agcggaacgg
    20821 aacaggcccg tcagctatcc cgtccagaaa ctgacgtacg cgcggatcag gattcgcctg
    20881 caacgcctgg gcactgccat gagcgacaat ttttttgtcc gccaggatcc aggcgtgatc
    20941 cgcaatactt aacacttccg gcacatcgtg agaaaccacc acacaagtca cgcccagcgc
    21001 gctgttcagc tcagaaatca gcttcaccag tacgcccatg gtgatgggat cttgcccaac
    21061 aaaaggttca tcaaacatga tgagatccgg ctccagcgca atcgcacgcg ccagcgctgc
    21121 acgccgcgcc atcccaccgg aaagttcaga aggcattagt ttagccgctc cacgcagccc
    21181 cacggcctcc agcttcatca tcaccgtact atgcaacaat ggcgcgggta gttgggtatg
    21241 ttcgcgcagt ggataggcga cgttgtcaaa tacgttcata tcagtgaaca acgccccgga
    21301 ctgaaataac atgctcatcc gtttgcgcac tgtatacagg cgcgaacgag acatcgccgg
    21361 aatattctca ccatcgaaaa ggatctcacc atgatctggt gcgatttgcc cgccaatcag
    21421 acggagtagc gtcgttttac cgatgcccga tggccccatg atcgccgtga tcttccctcg
    21481 cggcacggtc agggaaatat tatcgaagat gcagcgattg ccacgcgtaa aactgacatc
    21541 gcgcatatcg actaaattcg ccacagactg ctccataatt cacccttcgt cttgcgttga
    21601 ttttctaagc atggcgctca atttaacctt gaacccaaca tatttacaga atattacccg
    21661 ccgtggttag cgaaagctgg catttgtttt actttttagc cgcataaagt caaaattaag
    21721 aatccgttac ggctttctga aaatcttcag cggaccgacg agtatacctg aagaaaggac
    21781 gttagatgct tttagctacg gcactgttaa ttgttggttt acttttggtc gtttacagtg
    21841 ccgaccgcct ggtttttgcc gcgtctattc tttgccgaac ctttggcatc ccgccgctga
    21901 tcatcggcat gacggtggtc agtattggta catcgttacc agaaatcatc gtctcgcttg
    21961 ccgcgtctct gcacgaacaa cgcgatttag ccgttggtac agccctcggc tcaaacatta
    22021 tcaatatttt gctgatcctc ggtctggctg cgctggttcg tccttttacc gtccattccg
    22081 atgttctgcg tcgtgaatta cccttaatgt tgttggtcag cgtagtggcc ggttccgtac
    22141 tctatgacgg acaacttagt cgcagcgatg gtatctttct cctctttctg gctgtgctat
    22201 ggctgctgtt cattgttaaa cttgcacgtc aggctgaacg tcaggggact gacagcctga
    22261 ccagagagca gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc
    22321 tcggcattgc gcttatcatc atgccagtgg ccacgcggat ggtggttgat aacgccacgg
    22381 tgctggcgaa ttactttgcc atcagcgagt tgacgatggg tctgacggca attgctatcg
    22441 gaaccagcct gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca
    22501 ttgctgtcgg aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac
    22561 ccgcgctgat aacgccagga gagattgagc cattggcgta cagtcgtgac tacagcgtga
    22621 tgttgctggt gagcattatt tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc
    22681 gtggtgtagg ggtattatta actggcggat ttatcgtatg gctggcgatg ttgtactggt
    22741 tatcgccaat actcgttgaa taactggaaa cgcattatgt cgcacgtaga gttacaaccg
    22801 ggttttgact ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg
    22861 gagcttgatc aatacatcaa tcagaatttc acgcttgcct gtgaaaagat gttctggtgt
    22921 aaagggaaag ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg caaaatggcc
    22981 gcaacgtttg ccagcaccgg tacaccttca tttttcgtcc atcctggtga agccgcgcat
    23041 ggtgatttag gcatggtcac cccacaggat gtggtgattg ctatctctaa ctcaggtgaa
    23101 tccagcgaaa tcacggcctt aattccagtg cttaagcgtc ttcacgtacc gttaatctgc
    23161 atcaccggtc gcccggagag cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa
    23221 gtagcgaaag aagcctgtcc gttagggctg gcaccgacca gcagcaccac cgccacgctg
    23281 gttatgggcg atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat
    23341 tttgcgctct cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat
    23401 attatgcata cgggcgatga gatcccgcat gttaagaaaa cggccagtct gcgtgacgcg
    23461 ttgctggaag ttacccgcaa aaatcttggt atgactgtca tttgcgatga caatatgatg
    23521 attgaaggca tctttaccga cggtgattta cgccgtgtct tcgatatggg cgtggatgtt
    23581 cgtcagttaa gtattgccga tgtgatgacg ccggggggaa tacgtgtgcg ccctggcatt
    23641 ctggccgttg aggcactgaa cttaatgcag tcccgccata tcacctccgt gatggttgcc
    23701 gatggcgacc atttactcgg agtgttacat atgcatgatt tactgcgtgc aggcgtagtg
    23761 taaagattca aggataaaca acaatgagca aagcaggtgc gtcgcttgcg acctgttacg
    23821 gccctgtcag cgccgacgtt atagcaaaag cagagaacat tcgtctgctg atcctcgatg
    23881 tcgatggcgt actgtcagat ggcctgattt atatgggcaa taatggcgaa gagctgaaag
    23941 cgttcaatgt tcgtgacggt tatggcattc gttgtgcgct cacctctgat attgaagtcg
    24001 ctatcattac cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca ttggggatca
    24061 ctcacttgta tcaggggcag tcaaacaaac tgatcgcctt tagcgatctg ctggaaaaac
    24121 tggcgattgc cccggaaaat gtggcttatg tcggcgatga tctcatcgac tggccggtaa
    24181 tggaaaaagt gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg
    24241 ccgattacgt gacgcgcatt gctggcggtc gtggcgcagt gcgcgaagtt tgcgacttat
    24301 tactcctggc gcagggcaaa ctggatgaag ccaaagggca atcgatatga gtaaagccag
    24361 acgttgggtt atcattgtgc tatcactggc ggttctggtg atgatcggca ttgatatggc
    24421 cgaaaaagac gataccgccc aggtggtcgt caacaacaat gatcccacct ataaaagcga
    24481 gcatacggac acgctcgtct ataacccaga aggggcacta agctatcgat tgattgctca
    24541 acacgttgaa tattattccg atcaggccgt ttcgtggttt acgcagccgg tacttaccac
    24601 gtttgataag gataaaatcc cgacatggtc cgtaaaagca gataaagcca agctgaccaa
    24661 tgaccggatg ctctatttat atggacacgt tgaagtcaac gcacttgtgc caaactctca
    24721 acttcgcaga atcacgacgg ataacgcgca gatcaatctg gtgacgcagg atgttacctc
    24781 tgaagacctc gtcacgttat acggaacaac atttaactcc agcggtctga aaatgcgcgg
    24841 caacttacgc agcaagaacg ccgagctgat tgaaaaggtt agaacatcct atgaaattca
    24901 aaacaaacaa actcagcctt aatcttgtgc ttgccagctc acttctggcc gccagcattc
    24961 cggcatttgc cgtaaccgga gacactgatc agccgatcca cattgaatcg gaccagcaat
    25021 ctcttgatat gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc acccagggca
    25081 ccatcaaaat taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag
    25141 aagtgattga cggctacggt aaaccggcaa cgttctacca gatgcaggac aacggtaaac
    25201 ccgttgaagg tcacgcttcc cagatgcact acgaactggc aaaagatttt gtcgttctga
    25261 cgggtaatgc ttatctgcag caggtcgata gcaacattaa gggcgataag atcacttacc
    25321 tggtaaaaga gcagaaaatg caggctttca gcgacaaagg caagcgcgta accaccgttc
    25381 tggtgccgtc gcagctgcag gacaaaaaca acaaaggcca gaccccggca cagaagaagg
    25441 gtaattaatt cgttatggca acattaactg caaagaacct tgcaaaagcc tataaaggcc
    25501 gtcgcgtggt agaagacgtc agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg
    25561 ggccaaacgg tgccggtaag accaccactt tctacatggt tgtaggcatt gtgccgcgcg
    25621 atgcgggcaa catcattatt gatgatgacg atatcagtct gctgcctctg catgcacgcg
    25681 cgcgccgcgg tatcggctat ctgccacagg aagcctccat tttccgtcgc ctcagcgttt
    25741 acgataacct gatggcggta ctgcaaattc gtgacgactt gtctgctgaa caacgtgaag
    25801 accgcgcgaa cgagctgatg gaagagtttc acattgagca cctgcgtgac agcatggggc
    25861 agtcactctc cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc
    25921 cgaaatttat tctgctcgac gaaccgtttg ccggggttga cccgatctcg gttatcgaca
    25981 ttaaacgcat cattgagcac ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca
    26041 acgtgcgtga aacactggcg gtttgtgaac gcgcttatat cgtcagtcag gggcatttga
    26101 tcgcccacgg cacgcctaca gaaatcttac aagacgaaca cgttaagcgt gtataccttg
    26161 gggaagactt cagactctga tagggtagaa gtttgcgacg ttttagcagg agagtacgat
    26221 tctgaacatg aagcaaggtt tgcaactcag gcttagccaa caactggcga tgacgccaca
    26281 gctccaacag gcaattcgtc tgttgcagtt gtcgacgctg gaacttcagc aggagctaca
    26341 gcaggcgctg gagagtaatc cgctgcttga gcaaatcgac actcatgaag aaatcgacac
    26401 ccgcgaaacg caagacagtg aaacgctgga caccgccgac gcgctcgaac aaaaagagat
    26461 gccggaagag ctgccgctcg atgccagttg ggacaccatt tacaccgctg gtacaccatc
    26521 cggcaccagc ggtgactaca ttgacgatga gctgccggtc tatcagggcg aaacgacgca
    26581 gaccttgcag gattacctga tgtggcaggt cgagctgaca ccgttttccg acactgaccg
    26641 cgctattgct acctctatcg tcgatgccgt tgatgacatc ggttatctga ctgtcccgct
    26701 ggaagatatt ctcgaaagta tgggcgatga agagatcgac atcgacgagg ttgaagccgt
    26761 ccttaagcgg atccaacggt ttgatccggt cggtgtagcg gcaaaagatc tgcgtgactg
    26821 cctgctaatc caactctccc aattcgataa aaccacgccg tggctggaag aggccagact
    26881 gatcattagc gatcatctcg atctgttagc caatcacgac ttccgcactt taatgcgcgt
    26941 cacgcgtctg aaagaagatg tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc
    27001 gcgccccggg cagtcgatcc agactggcga acctgagtat gtcattccag atgtgctggt
    27061 gcgtaagcat aacggtcact ggacggtaga actcaacagt gacagcattc cgcgtctgca
    27121 aatcaaccag cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg acagccagtt
    27181 tatccgcagc aatctgcagg atgccaaatg gttgatcaag agtctggaaa gccgtaacga
    27241 tacgctactg cgcgtgagtc gctgtatcgt tgaacagcag caagccttct ttgagcaagg
    27301 tgaagaatat atgaaaccga tggtactggc cgatatcgcc caggctgtcg aaatgcatga
    27361 atcgacgata tctcgcgtga ccacgcaaaa atacctgcat agtccacgag gcatttttga
    27421 actgaagtat ttcttttcca gtcacatcaa taccgagggc ggcggcgaag cttcctccac
    27481 ggcgattcgt gcgctggtga agaaattaat cgcggcggaa aacccagcga aaccgttgag
    27541 cgacagcaag ttaacctctt tgctgtcgga acaaggtatc atggtggcac gccgcactgt
    27601 tgcgaagtac cgagagtctt tatccattcc gccgtcaaac cagcgtaaac agctcgtttg
    27661 acccaaccga taaggaagac actatgcagc tcaacattac cggaaataac gtcgagatca
    27721 ccgaggcact gcgcgaattt gttacagcca aatttgccaa acttgagcaa tattttgacc
    27781 gaatcaacca ggtctatgtt gttctgaaag tggagaaagt cacccacacc tcagatgcaa
    27841 cactgcatgt aaacggtggt gaaattcatg ccagcgcgga aggtcaggac atgtacgctg
    27901 ccattgatgg cttaattgat aagctggcac gccagttgac caaacataaa gataaactga
    27961 aacaacacta attgtccggg caattagcat gtgcatggcg gcctgttgtg cggcacaacg
    28021 ggccatttgt acggttaatg ctccgagcct gttccactgt ttgagtgggc aggttcttag
    28081 gtgaaattat gacaaataat gatacaactc tacagcttag cagtgttctt aacagggaat
    28141 gtacgcgaag ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc
    28201 tggcggcgaa acaacttagc ctgccgcctc aggtggtttt tgaagctatc ctgacgcggg
    28261 aaaaaatggg cagtaccggt atcggcaatg gtattgccat tccgcatggc aaactggaag
    28321 aagatactct gcgcgccgtt ggcgttttcg ttcagctcga aacgcctata gctttcgatg
    28381 ccatcgacaa ccaaccggtg gacttacttt ttgccctgct ggtgtcggca gaccaaacta
    28441 aaacgcacct gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc
    28501 gccgtttacg cgcagcccag agcgatgaag agctgtatca aatcattacg gataccgaag
    28561 gtactccgga tgaagcgtag ttattcggta atgtctcttt tagacgttgt gaggagaaac
    28621 agtacatggt actgatgatc gtcagcggac gttcaggttc aggtaaatct gtcgccctgc
    28681 gtgcgctgga agatatgggt ttttactgcg tggataacct tcccgtagtg ttgttacccg
    28741 atctggctcg aaccctggcc gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta
    28801 atatgccgga gtcaccagaa atattcgaac aggcgatgag taacctgcct gacgctttct
    28861 caccgcaact cctgttcctg gatgccgacc gtaatacctt aattcgtcgt tacagtgaca
    28921 cgcgccgact gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag
    28981 aaagcgattt gctggagcct ctgcgttcgc gagcggatct gattgtcgat acctcagaaa
    29041 tgtccgttca cgagctggct gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg
    29101 aactgaccat ggtctttgag tctttcggct tcaaacacgg tatccctatc gatgcagatt
    29161 acgtctttga cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg cgtccaatga
    29221 caggtcttga taaacctgtc gccgcgttcc tcgaccgcca cacagaagta cacaatttta
    29281 tctaccagac gcgaagctat cttgagctat ggttacctat gctggaaacc aacaaccgta
    29341 gctacttgac ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg tatattgcag
    29401 agcaactggc agactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc
    29461 tggaaaaacg taaaccatga ccgtcaagca aactgttgaa atcacaaaca agctgggcat
    29521 gcatgcccgg cctgcaatga agctgtttga attaatgcag ggttttgacg ctgaagtgct
    29581 gttacgtaat gacgaaggca ccgaggccga agccaacagc gttattgcgc tgctgatgtt
    29641 ggattctgcc aaaggacggc agattgaagt tgaagcgacc ggcccacagg aagaggaagc
    29701 actggccgcc gttatcgccc tctttaactc tggttttgat gaagattaat cttcatcact
    29761 ttgacataca actcccttca aactcccccc gacaataaga aaatcacgta ctgaaatcgt
    29821 tctcaatcaa cgtcatttgt acattttgtg cgcttttcac tttcagaaga accttaagaa
    29881 aaccttaaga ggcattgttt aggttttgtt taagttaatc gaccatactg gagatcgtca
    29941 gaaaatattt ccaggagatg gcatgattcg cttatctgaa caaagtcccc tgggcaccgg
    30001 aagacatcgg aaatgttatg cgcatccgga agatgcccaa cgctgtatca agattgtcta
    30061 ccatcgtggc gatggtggcg ataaagagat ccgccgtgag ttaaagtact acgcgcatct
    30121 tggtcgccgg ttaaaagact ggagtggaat accgcgctat cacggtaccg ttgagacgga
    30181 ttgcggaacc ggttatgtct acgatgtgat tgcagatttt gacggcaaac tctccattac
    30241 gctgaccgaa tttgctgaac aatgccgtta cgaagaagac attgcacaac tgcgccagct
    30301 actgaaacag ctgaagcgtt atttgcagga taaccgtatc gtgacgatgt cgctgaagcc
    30361 gcagaatatc ctctgtcacc gcatcagtga gtcagaggtt atcccggtcg tctgcgacaa
    30421 cattggtgaa agcacattga ttccactggc gacctggtcg aagtggtgtt gtctgcgtaa
    30481 gcaagagcgg ttatggaaac gatttatcgc ccaaccggcg ctggctattg ccctgcagaa
    30541 agatctgcaa cctcgtgaga gcaaaacgct ggccctcact tcgcgcgaag cttaatccag
    30601 ctggtgttgt tgcataaacg gctcaccgcc taactgatac atctgccgta aaatccacgc
    30661 ctgacggcta cgcacgtagc ccgatggcga ggagactttg aaacgaagtg gattaggtaa
    30721 tacagctgcc agtaatgcag cttccgaccg ggtaagtttg ctcgcgggtt tgtggaaata
    30781 acgttgtgcc gcagcttcga cgccaaacac accgtcgcca aattcggcga tattcaggta
    30841 aaccgtcagg atacgctttt tgctccagac tgtttctatc cccagcgtta atccagcttc
    30901 cagccctttt cgtacccagc tacgcccatc ccataaaaag agatttttgg ccgtctgctg
    30961 agaaatcgtt gaagcaccgc gaatacggtt ttcattgcgc tcgttgtgcg ccagggcttt
    31021 ctcaatggaa gcgacatcaa agccccagtg ctcagaaaat ttctgatctt ctgcggcaat
    31081 caccgccagt cccatccacg gcgagatttg atccatactg acccagtcag aatgtgccac
    31141 gtaacgaaaa ttgccatgca gccaggcgct gacctgtcgc tcgaccatta ccgctgagaa
    31201 gggaacaggc gcaacactaa acaacgcgat gcccccgccc cagaaaacgg cgaggacaac
    31261 cattaacagc aaaagaaaac ggcgaacgaa actaaacacc gttaagcggc ttttactcat
    31321 tcagccagaa ccagcacgcg ggaaaccagc ttatcaatgc cgctcgccgc ttctgcaatg
    31381 ttctgcgcca gcatatatgc tggggtggtg acaatcttat tgtcttcatc aaccacgata
    31441 tcatcgacag gacagggcac atgctccgcg cccatctctt ccagcacttc tgcggtatcg
    31501 atatcagtac cgatggtcaa acgcagcggg aaatcgaaaa ttttcggcag catcgccggg
    31561 gcaatacaca taaaaccaag cggttttccg gcctgatgca tcgcttgtgc cagcgccttt
    31621 aattcacggt caacggtgca ttcgctacca agagtggcaa aattgcttaa attcttcgcc
    31681 gcgccaaacc cccccggcac aatcaacgca tccagttcag cggcatcggc ctgggccaga
    31741 ggacggattt caccgcgcgt tattcgtgcc gcttcaatca gcacgttgcg cgtttccgtc
    31801 atcgcttcgc cagttaaatg gttgataaca tcaacctgct gcttatccgg tgcaaagcag
    31861 accgcctgcg caccgctgcg tgaaatagct aacaacgtca acaccgcttc atgaatttca
    31921 gaaccgtcat agacgccgca tccgctcaga attacgccaa ttttcttcat tgtgatcatc
    31981 ctttcgcaat tgactgaaac acattaattt ttttaataaa aatggtacgc atcacacatt
    32041 taactgattc atgtaacaaa tcatttaagt tttgctatct taactgcgtg cggcctgaaa
    32101 aacagtgctg tgcccttgta actcatcata ataatttacg gcgcagccaa gatttccctg
    32161 gtgttggcgc agtattcgcg caccccggtc tagccggggt cattttttag tggcttttgc
    32221 cacccacgct ttcagcactt ctacgtcgtg acgccactct tctttcatct cttcaatcca
    32281 ttcaccgacg ttatcttccc aggcaggaag gtcaggagac tgaatttgct gacccagctg
    32341 ttgcagatgg cgtaacccca ctgaccccgc cgcaccttta attttatgtc cttcctcaac
    32401 aatgcctttt ttatcctgcg ccgtcagatt cgactccagc acgctgacat agcccggcat
    32461 catcttctca aacaccgcta acccgtcggt gatcagcttc ggtcctacaa gttcgagata
    32521 ctgttccagc atgggaatat cgagcaatgc ttctgattta ctgttctctt ctgtcgtcac
    32581 cgtactctcc tcatcatcct gggtatccca gaatttcttg atcatcgcgg ttagcgccgg
    32641 aacagaaagc ggcttactca gcacatcatc cattccagca ttgaggtact cttgtttgtc
    32701 tttcagcacg ttagcggtta aggccaccag cggcggtaaa tcctctcgcg gataacgctt
    32761 cgtcagttca cgagagatat ccagcccggt catatctggc aactgaatat ccagtaacac
    32821 caggtcgtat tcgcccggtt taaacatctc tagcgccgcc ttgccggtca tggcgacatc
    32881 aacgctgtta cctaattttt ccagcacaga acgcgcaaca atcacgttca gttcaatgtc
    32941 ttccaccagc agcacattca gcgccggtaa aggcatatcg tcttcatcaa acgcatcatc
    33001 gacctcttct gctaccgacg gtgcgtggat cgtcaacgta aaggttgaac ctttgccctg
    33061 ttcgctggta accgtaatat cgccgcccat atttttcgcc agacgacgag aaacggccag
    33121 accaataccg gtgccggtgg caggtttacc gccatgactg tctttcacct ggtaatacat
    33181 ggcgaaaatt ttatccagct catcctgcgg aatgccgata ccagagtctt ccacttcaaa
    33241 atgcagcata tcgccttcat cgtagcgcac gcgcacggta acctggcctt gctgggtgaa
    33301 tttgacggcg ttactgatga ggttccacag gatctgccgt aaacgcgtcc cgtcggtaat
    33361 gacctgatgc ggtaatggca gcgtcggctc caggttaaag cgcaatcctt tttgttgcgc
    33421 ctgcaatgcg gagagatttt ccagatcggc aaggaagctg gtgaaatcaa ccggttgatt
    33481 atcaagctgg accttgcgcc gttccatctt atccatgtcg ataatatcgt taaagatatt
    33541 ccccagcgtg acggccgaaa catggatggt cttgagatat ttttcctgct cggcggtgag
    33601 ttcggtatcc agcagaatgc ggctcagacc gacgataccg ttcagcggtg tacgcaattc
    33661 gtgactgatg gtggagataa acgtcgtttt gtcgcggctg gcccgttcaa gcgcatcctg
    33721 ataccgctta cgctcggtaa tgtctcgacc aaagcccatc aaaccgtgac gtttacccac
    33781 gcggtcgtag tacggcactt tacggatttc aaagcaggct ttgcgcccgt ccgggtaatc
    33841 cagccactgt tcataggtca gtgacacatt atgacggaac actttttcat cggtttcaat
    33901 gacttttgcg gcggcttccg gtgagtaaac atcaacaggt ttcaggtgaa ccagttgttt
    33961 ttcgcttttt ccggtcagca gctccatcgc gcggttacag ccggaaaact ctttatcttc
    34021 gttacgataa aaaaccaggt cgggtgaagc atcaaggaag gaacgtaaga atgaggattg
    34081 ctgctcgagc tgaatttgtg tctcttcgcg ctctttgatt tcaattttca gttggccgaa
    34141 ggtttcctgc agttctgctt ccgctttttc acgaacggcg atttcctgat ttagctgggc
    34201 aatattatct tttaactgaa cgttgaggct caaatcgcgc tcgcgcatct cctccagttt
    34261 ttgcaccagc cgtgacagac gttgtcgtga ctcctccagt tgctcgacga ccaccgatag
    34321 aaagtagacc gcccacggcg taatcaacaa accaaagaag atagaacgaa taacatcaat
    34381 gctttcgacc tgaccatgca gcaccatggt taccgccatt tgtaccacaa tggcaagaac
    34441 gacgagggcc agcgccagca acattgagaa gcgcaccaga cctaacttca tcatcaggtc
    34501 aacataatac tgcgccagca gacgaatttg cttcattagg gaattccttc acgacaacct
    34561 gccccaataa tacccaattc tgacagttac gttataaatt gtgtgagaaa tgcggaattc
    34621 ccctcaacga cctactccgt tggaggtagc cacggacgtc ccagcgccga tccctgtacg
    34681 ccatgttcat tcagatagcg atccagctcg accattcccg tccatcgatt ttcgcaccac
    34741 agaggagcaa gcagcgtcgg gcgacgggcg ctggcggaaa tgcggtggta gatcacttcc
    34801 ggtggcgtat ggcgaatcat ttctccggca gtgagcgtgt aatcctccag ttcaataccg
    34861 ttcaaacgcc ccgcttccca ggctttcgcc ataatgctgc ctttcacaat atgcaacgga
    34921 tgtagcttta taccgtcaac accggtttcc actacccgct ccagcgtttg cagacattca
    34981 gccagccctt cgcctggcag accgacaatt aaatgcgagc acaccttcag cccgcgctgg
    35041 cgtgccagct gcgtcgtgcg ctgataacag gcgaaatcat gaccgcgatt aatgcgatgt
    35101 agcgttttgt cgtgcacggt ttgcagcccc agctccagcc acacttcgta gccctggtct
    35161 ttatattcgc aaagcagatc cagcaccgca tccggcacgc agtccgggcg cgtgccgaca
    35221 cacaaaccga caatattggc ctggctcacc gcctgttgat acatcgaacg cagcacctga
    35281 acttcggcaa aggtgctggt atacgcctgg aaataggcga ggtaacgttt cgcgcgatta
    35341 attaaattcg cctgatgcgc cagctgttcg gcaatggaac gatgctgctg cgcttcgtcg
    35401 gcaaacgagg caacattaca gaaggtgcag ccgccgcgcc cgatggtgcc gtcgcgattc
    35461 gggcaactga aaccgccgtg cagcgtcagc ttatgcacct tttgcccata acgacgggtg
    35521 agatcaccac caaacatatt gactaatttc tgtaactgca taatctgata gaccgcgcct
    35581 tgaaaagagg ccaaagcctg ccatttttag ctcaattcgg cgatgacctg gatcaatcgt
    35641 cccggcccgc ttttatcaac tgcataatca atcaaaatta ccgaaatttc atgcataatc
    35701 acataaatca cttttgctta tcttgtgtca gattttttta tctccttatg gattttaggc
    35761 aaaaacagta gcatgaaacg tcattaccaa ttaatgcagt ataaaatgct ggttttctcg
    35821 tcaattcaag gcaggataag ggttaacacg cctttatgac agtcaggaat tgactgtttc
    35881 tctaacgact tcccttttag ccttaaagat aaaatctatt ttaatttcag ccatttaata
    35941 aagaattttg cgctaaagca catttctgta ccaataagct tgccatttga cctgtatcag
    36001 ctttcccgat aagttggaaa tccgctggaa gctttctgga tgagcagcct gctcatcata
    36061 tttatgcagt aattgagatc ccctcttcac cgtattaacc gatgcgaaaa ggacaacaag
    36121 ggggcgaatg cgaggcgcgc gtatgacacg caacccccgt cgccacgctc tttctgtacc
    36181 cgtgcgcagc ggttcggtag tggggttccc gcagagcctg ggggaggttc acgatatgtt
    36241 gtacgataaa tcccttgaga gggataactg tggtttcggc ctgatcgccc acatagaagg
    36301 cgaacctagc cacaaggtag tgcgtactgc aatacacgca ctggcccgca tgcagcaccg
    36361 tggcgcgatt ctcgccgatg gtaaaaccgg tgacggttgc ggcttgctgt tacaaaaacc
    36421 ggatcgcttt tttcgcatcg ttgcgcagga gcgcggctgg cgtttagcga aaaactacgc
    36481 cgtcgggatg ctattcctga ataaagatcc tgaactcgcc gctgccgcac gccgcatcgt
    36541 tgaagaagaa ctgcaacgcg aaaccttgtc gattgtgggc tggcgtgatg tccccactaa
    36601 cgaaggcgta ctgggtgaaa tcgccctctc ctctctgccc cgcattgagc aaatttttgt
    36661 gaacgccccg gcaggctggc gtccgcgcga tatggagcgc cgtctgttta tcgcccgccg
    36721 ccgcattgaa aagcgtctcg aagccgacaa agacttctac gtctgtagcc tatcgaatct
    36781 ggtgaacatc tataaaggtc tgtgtatgcc ggcggatctg ccgcgctttt atctggatct
    36841 tgcggacctg cgtctggaat cggccatttg cctgttccac cagcgcttct ccactaacac
    36901 cgtaccgcgc tggccgctgg cgcaaccgtt ccgctatctg gcgcataacg gtgaaatcaa
    36961 caccatcact ggtaaccgcc aatgggcgcg tgcgcgtacc tataaattcc agacgccgct
    37021 tatccctgat ctgcacgacg ccgcaccgtt cgtcaacgaa accggctctg actccagttc
    37081 gatggataac atgctggaac tcctgctggc aggcgggatg gatatcatcc gcgccatgcg
    37141 tctgttagtg ccacccgcct ggcagaacaa cccggatatg gacccggaac tgcgtgcctt
    37201 ctttgacttt aactccatgc atatggagcc gtgggatggc ccggcgggca tcgtgatgtc
    37261 cgacggtcgt tttgccgcct gtaacctcga ccgtaacggt ctgcgtccgg cgcgctacgt
    37321 catcaccaaa gataagctca tcacctgcgc ctctgaagtc ggtatctggg attaccagcc
    37381 tgacgaagtg gtcgaaaaag gccgcgtcgg gccaggcgaa ctgatggtta tcgacacccg
    37441 cagtgggcat attctgcact cggcagaaac cgatgacgat ctgaaaagcc gccatccata
    37501 taaagagtgg atggagaaaa acgtccgccg actggtaccg tttgaagatc tgcccgatga
    37561 agaagtgggt agccgcgaac tggacgacga cacgcttgcc agctaccaga aacagtttaa
    37621 ctacagcgcg gaagagctgg actccgtaat tcgcgtactg ggcgaaaacg gtcaggaagc
    37681 ggtcggttcg atgggcgatg ataccccatt cgccgtgctc tccagtcagc cgcgcattat
    37741 ttacgactac ttccgccagc agtttgccca ggtgactaac ccgccaatcg acccgctgcg
    37801 tgaagcgcat gttatgtcgc tcgccaccag tatcggtcgt gaaatgaacg tcttttgcga
    37861 agcagagggc caggcgcacc gtttaagctt taaatcgccg attctgctct actccgattt
    37921 caaacagctc acgacgatga aagaggagca ctaccgcgca gatacgctgg atatcacctt
    37981 tgacgtcact aaaaccacgc tcgaagcgac agtcaaagag ctgtgcgaca aagccgaaaa
    38041 aatggtacgt agcggcaccg tgctgctggt gctctccgac cggaatatcg ctaaagatcg
    38101 cctgccggtt ccagccccga tggcggttgg cgcgatccag acccgtctgg tcgatcaaag
    38161 cctgcgttgc gatgccaaca tcatcgtcga aaccgccagc gcccgcgatc cgcaccactt
    38221 cgccgtgttg ctgggcttcg gcgcgacggc tatttatcca taccttgcct atgaaacgct
    38281 gggccgcctg gtagacaccc atgcgattgc caaagattat cgtaccgtga tgctcaacta
    38341 ccgtaacggc atcaacaaag gcttgtacaa aatcatgtcc aaaatgggca tctccaccat
    38401 cgcctcttac cgctgctcga aactgtttga agcggtcggt ctacacgatg atgtagtggg
    38461 cctgtgcttc cagggggcgg tcagccgcat tggtggggca agctttgaag acttccagca
    38521 ggatctgctg aatctgtcga aacgtgcctg gctggcgcgt aagcccatca gccagggcgg
    38581 cctgctgaaa tacgtccacg gcggcgaata ccacgcctac aacccggacg tggtgcgcac
    38641 gctgcaacaa gcggtacaaa gcggcgagta cagcgactat caggaatacg cgaagctggt
    38701 taatgagcgt ccggcaacca cgctgcgcga tctgctggca attacgccgg gtgaaaacgc
    38761 ggtcaacatt gctgatgttg aaccggcaag cgaactgttt aaacgcttcg ataccgctgc
    38821 gatgtctatc ggcgcgttaa gcccggaagc ccacgaggcg ctggcggaag cgatgaacag
    38881 catcggcggt aactcaaact ccggtgaagg cggtgaagat ccggcgcgct acggcaccaa
    38941 caaagtgtcg cgcatcaagc aggtggcttc cggtcgcttt ggggttactc cggcgtatct
    39001 ggtcaatgcc gacgtcattc agattaaagt cgcccagggc gcgaagccgg gcgaaggcgg
    39061 tcagttgccg ggtgataaag tcactcctta catcgccaaa ctgcgctatt cggtgcccgg
    39121 agtgacgctg atctccccgc cgccgcacca cgatatctac tctatcgagg acttagcgca
    39181 gctcattttc gacctcaagc aggttaaccc gaaagcgatg atctccgtga agctggtttc
    39241 cgaaccgggc gtaggcacca tcgcgactgg cgtggcaaaa gcttatgccg atttaatcac
    39301 catcgcaggc tatgacggtg gcaccggcgc aagcccgctg tcgtcagtga aatacgcagg
    39361 ttgcccgtgg gagctggggc ttgttgaaac ccagcaggcg ctggttgcca atggtctgcg
    39421 tcataagatc cgtttgcagg tcgatggcgg cctgaaaacc ggtgttgata tcatcaaagc
    39481 ggcgattctc ggcgcagaaa gcttcggctt cggcactggc ccaatggtcg cgctcggctg
    39541 taaatatctg cgtatttgcc acctgaacaa ctgcgcaacg ggtgtggcaa ctcaggatga
    39601 caaactgcgt aagaatcact atcacggcct gccgttcaag gtgacgaatt actttgagtt
    39661 tatcgcccgt gaaacccgcg agctgatggc acagctgggg gtaacgcggc tggtggatct
    39721 gattggtcgc accgacctgc tgaaagagct ggacggtttc accgccaaac agcagaaact
    39781 ggcgctgtcg aagctgctgg agaccgccga accgcatcca ggtaaggcac tctactgcac
    39841 cgaaaacaac ccgccgtttg ataacggcct gctgaacgcg cagttgctgc aacaggcgaa
    39901 accgtttgtc gatgaacgcc agagcaaaac cttctggttc gatatccgca ataccgatcg
    39961 ctccgtgggc gcgtcgcttt caggctatat cgcccagacg cacggcgatc aggggctggc
    40021 agccgatcct atcaaagcgt acttcaacgg caccgcaggc cagagtttcg gtgtatggaa
    40081 cgcgggcggc gtggaactgt acctgaccgg ggatgccaac gactatgtcg gtaaaggcat
    40141 ggcgggcggc ttaatcgcca ttcgtcctcc ggttggttcc gccttccgca gccatgaagc
    40201 aagtattatc ggcaacacct gcctgtatgg cgcgaccggt ggtcgtctgt atgccgcagg
    40261 ccgcgcgggt gaacgtttcg gcgtgcggaa ctccggtgct atcaccgtgg tagaaggcat
    40321 tggcgacaac ggctgtgaat atatgacggg cggtattgtc tgtattctgg gtaaaaccgg
    40381 cgttaacttc ggtgcgggca tgaccggcgg cttcgcttac gttctcgatg aaagcggtga
    40441 tttccgcaaa cgcgttaacc cggaactggt cgaggtctta agcgttgacg atctggcgat
    40501 ccatgaagag catctgcgcg gtcttatcac cgagcatgtt cagcatactg gctctcagcg
    40561 cggtgaagag attctggcga actggtcaac cttcgccact aaatttgcgc tggttaaacc
    40621 gaagtccagt gatgtaaaag cactgctggg tcatcgtagt cgtagcgcag cagagttgcg
    40681 cgtgcaggcg cagtaagggg tagcaacaat gagtcagaat gtttatcaat ttatcgacct
    40741 gcagcgcgtt gatccgccaa agaaaccgct gaagatccgc aaaattgagt ttgttgaaat
    40801 ttacgagccg ttttccgaag gccaggccaa agcgcaggct gaccgctgcc tgtcgtgcgg
    40861 caacccatac tgcgagtgga aatgtccggt acacaactac atcccgaact ggctgaagct
    40921 cgccaacgag gggcgtattt ttgaagcggc ggaactgtcg caccagacca acaccctgcc
    40981 ggaagtttgc ggacgagtct gcccgcaaga ccgtctgtgc gaaggttcct gcactctgaa
    41041 cgatgagttt ggcgcggtga ccatcggcaa cattgagcgt tatatcaatg ataaagcgtt
    41101 cgaaatgggc tggcgtccgg atatgtccgg cgtgaagcaa accggtaaaa aagtggcgat
    41161 tatcggcgca ggcccggcag gtctggcgtg tgcggatgtc ctgacgcgta acggcgtaaa
    41221 agccgttgtc ttcgaccgtc atccagaaat tggcgggctg ctgaccttcg gtattccggc
    41281 cttcaagctg gaaaaagagg taatgacgcg tcgccgtgaa atcttcaccg gcatgggtat
    41341 tgaattcaaa ctcaataccg aagtgggccg cgacgtgcag ctggacgatc tgctgagtga
    41401 ttacgatgcc gtcttccttg gcgtcgggac ttatcagtca atgcgcggcg ggctggaaaa
    41461 cgaagacgcc gatggcgtgt acgcagcgct gccgttcctt atcgccaaca ccaaacagtt
    41521 aatgggcttt ggtgaaaccc gcgacgaacc gttcgtcagc atggaaggca aacgcgtggt
    41581 ggtccttggc ggtggcgaca ctgcgatgga ctgcgtgcgt acgtccgtgc gccagggagc
    41641 gaagcacgtt acctgtgcct atcgtcgtga tgaagagaac atgccgggtt cccgccgcga
    41701 agtgaaaaac gcgcgggaag aaggcgtaga gttcaaattc aacgtccagc cactggggat
    41761 tgaagtgaac ggtaacggca aagtcagcgg cgtaaaaatg gtacgtaccg aaatgggcga
    41821 accggatgcc aaaggccgtc gccgcgcgga gatcgttgca ggttccgaac atatcgttcc
    41881 ggcagatgca gtgatcatgg cgtttggttt ccgtccacac aacatggaat ggctggcaaa
    41941 acacagcgtc gagctggatt cacaaggccg catcatcgcc ccggaaggca gcgacaacgc
    42001 cttccagacc agcaacccga aaatctttgc tggcggcgat atcgtccgtg gttccgatct
    42061 ggtggtaacc gctattgccg aaggtcgtaa ggcggcagac ggtattatga actggctgga
    42121 agtttaagct ctgtcccagc gccgcctcct cagaaaagag tcggcgctga aattctcctt
    42181 tctttctgga aagcgcctcg cataatctga agaaaaccaa tgtaatacgc tcatctggat
    42241 tgttaccggt agatgcccgg cgtatagctg attccattat tcaacgcagc gccatataaa
    42301 gggacagata atggaatcgc tctcagagag aacctcaaca ggctaccagc aaatccacga
    42361 cggtattatt cacctggtcg atagcgcccg gacggaaacg gtacgtagcg ttaacgcgct
    42421 gatgaccgcg acatactggg aaattggccg acgaattgtc gaatttgaac aaggtggcga
    42481 ggcaagggct gcgtatggtg cgcagctaat caagcgacta tcaaaggatt taagtctaag
    42541 gtataagcgt ggtttctctg caaaaaactt acgccaaatg aggctttttt acctcttttt
    42601 tcaacatgtt gaaattcgcc agacagtgtc tggcgaatta acaccattgg gaattccgca
    42661 gacaccatct gcagaattcc catcggcaaa gatttggcag acgctgtctg ccaaattgag
    42721 aggctgtttg atatttccac agcttttgta ttgcatctcc tattttcggt ttgtatattc
    42781 atgcaaataa acgaaggaaa gttgccgtga tttacattga acagcttgaa ttaatccata
    42841 aaagtggtga tgtgttgtat ccagtaaaaa taaccagaaa atcatcagga aaaactgcct
    42901 tccaccttgt tccattcggc ttaaataaga ctcacgacct tctagaggtg gaagatgctt
    42961 cagaagcaat tcggctggtg attgatgaac gtcattccat tcgctgcagt acacttacag
    43021 caactattac gaataaaaag ggcaagcgaa ttaagcggac aggtatatac agtatcaagg
    43081 gtgttaacat aaaagaatac aacgtcaggt aatggcacag agcggtccgt cagttcaggt
    43141 ttggctctgt gccaaagatg ggtcaggtca cacctgatct aataaattta agccattaaa
    43201 tcagccttaa ccttcaccac caccttttta atctcacccg gctcgccaac aacgcatccc
    43261 ggcttatgcg gctctcctag cataaacacg gcgaacattc ccggtttcag gatgatggct
    43321 tgctcgtttt caatggtgct gcaaagctgg taatcatcct catggtggaa ctcttcacac
    43381 tgacgcgcag tgcctgccat gccaaacaga atccgttctt caccgtttaa taacagctgg
    43441 atatcaatgt attgctcgtg cagctccgct tttttctcaa cgggcgattg agtgttaaac
    43501 gtcatgacat tcataaaaat attgtcgccc tgtaattcgt aacgacccgg cgctttttct
    43561 tgcggtctgg cagctaatgc cagcgttaac gcgtcctgta acgcaggatg taacccagca
    43621 gacggtaatg accgtacttc acccatcatc ataatttttc tccctgggcc aacagcgctg
    43681 ccccaagtaa acctgcatca tggcggtagt gcgccgccag taaatcaaca tgaaataccg
    43741 ctggctcctg cgccagatac gtttccacca acgccagata cccttctgcc agaccaacgc
    43801 tgccaccgac caccacgcac tggcaatcag ttgtggcttt aatatcagcg atcagccttg
    43861 ccagcacatg tgcggagcgg tgaatcagtt gctgcgcctg ctcgtcgccc tgcccggcgc
    43921 gtgtgaaaat agttctcgca tccgcgccca tgagttcacc ttgcgctgcc gctgcaatac
    43981 cgcgaccaga agcaatcgct tccacgcaac ctgtgcgtcc acagccgcag actgggccgt
    44041 gtggatcggc aagcgtatgc ccgatatgcc ccgccagacc gccagggccg gtaagcagtt
    44101 taccgccgct cactacaccg ccgccgacgc cagtggaaac ggtgataaac accatctcgg
    44161 ttacatcgcc ttccagcgcc tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg
    44221 caatggtcgg caaattggta agttgttcca gcgttttgac taacggaaag tgtagcaatc
    44281 caccgagatt atgcggattc agcgccagca aactgccgtc gcgaataatt ccggttgaag
    44341 cgatggcaac ccgctgcgca tgagcttgca acggagagac taatgcggat aaggcatcac
    44401 gcaaggcttc tggtgtctgg ctggctggcg taggaagttc acgacgatcg cggatctgcc
    44461 cgtcagcgcc aatcagcgcg gcggcaagtt tagtaccgcc gatatcaatc gccagtgtgg
    44521 tcatagcacc gcctttttca tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt
    44581 aatggcagaa ccgaccgtca ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc
    44641 aggcgtgttg taacgccctt cggcaatcac ccgacatccg gcttcgctca acgttttcac
    44701 cagcgccaga tccggctctt ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc
    44761 aataatttcg gctcccagct tttggcatgc caggccgtct tccggcgttg agcagtcggt
    44821 catcgccagt aaaccgtgat gatgtatacg tgccagcagc gtttcaacag gcaccggacg
    44881 cgggcggtcg gtgccgtcaa tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac
    44941 atcttcaata taggccgtga tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc
    45001 aataatcggc acgctcacca ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg
    45061 aatggcaacc gcgcccgcct gctctgccgc taatgccatg gctgcgacga tttcgggttt
    45121 atcgagcggg ctgtcaggaa tcggctggca ggagacaatc aggccaccgt tagcagcgat
    45181 tttttgatcc agttgtgcaa gtaacgacat acatcttccc ttagcgaaag gcccggtaca
    45241 tagaccgggc aacaggatta acttttggtt ttgactaaat cgtttttggc gctgccaaat
    45301 ggcacggcac cgctaaatgg tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg
    45361 cgcagccaac gctgaacgcg ggaaggcata tccagaccaa tcagcaggat caccacaaat
    45421 gtcaggctga acgagagcga tgccagcgca gtacccagat ccagacgttg agcgatcaac
    45481 gcgccgatga ttggggccag tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg
    45541 cccgctgcac gctggtcggt atcgaaataa ccgccaatca gtttcggtaa gatcccggcg
    45601 atcccttgtc caagcatttg ctggaagaac agtaacagac cgagcaccca gacgtttgcg
    45661 ccgccaatcg caaataccgg aataatcagc agctgcgagg ccagcaggct acaaacgtac
    45721 gctttgcggg ttcccagcca gtcaccgagg aagcctccta cgcagcatcc cacggccgcg
    45781 ccaaagccac taaagaacag cacattggcg acagtatgcg ggttataagc cagatcggtt
    45841 ttcagatacg ttggcagcag cgcctgaatc ggccatgagt agaggaaagc aaacaacacg
    45901 accaccatca gcattacgcc cgttggccag cgtttgcctg tactctgcac cataaagctg
    45961 ataaagattg cggcgcataa cagcccaaga acagcgacga tcgcggcatt ttgcaggtta
    46021 ccggcgaagc agaaccacag cgcagtagcc gccgccagtg tcattacgat attggcaatg
    46081 cgatgttcac cacggtagag aatatccacc attgtgcgta ctggtgcttt acctgcgtgt
    46141 ttctctttcc agtcttccgc ttccgggatg tttttacgca gccagagagc aaagatgatt
    46201 ggcaaaatgc cgataaagaa cagcgcacgc caaccccaga ccggaaccac caggctatag
    46261 acctgagcgg caacgacggc ccccacagag aagcctgaaa tcaaaaaacc actggctttg
    46321 ttacgcagat gttttggcca gctttcaatg acataggtgg cgctggaacc gtattcaccc
    46381 gccatcccca tgccgatgac cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag
    46441 ccgcaggcca gcgtcccggc cgagaagaga acgatgctgg tgaccattgc cagacgacgc
    46501 ccgtagcggt cacccatagc gccgagcatc aggccgccga accagcgaga gataaaggct
    46561 gcggagatca gacttgccgc ctgcaccgtc gtcagcccga attcaccctg tacttcggtg
    46621 agtaccaggg cgattaaaac gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg
    46681 gaaaatgcgc gccattgtgc acggttgaga tggcgatacc acgggatatt ctgggttgta
    46741 gtactcattg tgagtctccc gcggtgggcg atgcccacac gctttggtat gaaaattgta
    46801 gggtacagat gcgtttattt cccctcaccc ggtaggggcg agcgagggga aaacaactca
    46861 cccgcgctct tgcatcaact gctgggccag cgccttcagt tctggcagat atttttcatc
    46921 taccggtcca aacggtttgc ggcacagcgg cacagaaacg acatccatat aatgcaggac
    46981 agttttcagg ccgcggaata cgccagtttt gatcagtaaa tcaatgactt tgttgcactc
    47041 agtttgcagt ttctgcgcgg tctggatatc gccttctttc agcgccttca cgatcccctg
    47101 atatcgccag cccatgatgt tgtaagtact gccgatacca ccatcagcgc ctgccagcaa
    47161 gccggaagcg aagatttcgt cgtaaccgtt atagagcacc agatcaggat gttcacgacg
    47221 gatctgttcc atctgataga gatcgccaga agtctgtttc agcgcaccga cgccaggcaa
    47281 cgtaaccagg gtgttgatct gatccagggt cagttttacc ccactcaggg ctggaatgtt
    47341 gtacacaacc atcggcaaac catccgctga atcaataatt gcccgatagt gatcgcagtg
    47401 ttcttcaaag ctgaaaggat agtagaacgg cgtgacggcg gagacggcat cgaagccata
    47461 acgtttagcc gatgccgcaa gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc
    47521 gatgagttta atcttacctt tcgcctcttc ggcgacgatt tccagtacct gttcacgctc
    47581 ggaaaggctt tgtacaaagg cctcgccggt cgaaccaccc acgtataaac cgtcgatgcc
    47641 ctgctgaata ttgaactgaa ccaggcgacg cagactcgct ttatccagtg cttgttgttg
    47701 gtcaaaagga gtcaggagtg cagccattac gccacgtaaa ttcgttgcca taaatacctc
    47761 tgaagtgata cttgtctgat aaacgatata cctttatacc tgttatacca gatcaattaa
    47821 gcaacacccc atacagaaag cttataatgc gatctgcttc actaaagtgg cattatttct
    47881 ttttgttggt ggtctgaccg aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt
    47941 gcaacgcacg atcggcttcg tcaggatcat gacggcggat cgcatcaacg atcgcaatat
    48001 gctgttgata gctaacgttg ttatgctcgt gcagagcctg atcagccacg gttggacgtg
    48061 cggcgatcag ccagtcgagc agggcaacgt ggatcgccat gaagattggg ttaccaggga
    48121 tctccgccag cacgcggtgg aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca
    48181 gcgactgact gttgatttcc agtgcttttg ccagcaaatc gatttgctca tcggtggcat
    48241 gttcagccgc atagcgcacc agactggatt caaagaacag acgtaattgt tcgaaatggg
    48301 caatcccacc gggatgagaa aggaaatctt tcgccatgcc ggaaaactca ccgatgatag
    48361 tgtccgcaga aggacgcgag acgcgagcgc gttcgccgtt gtttatttgc accagacctt
    48421 tgcgttttaa cgctgccagc gcttcgcgca cagaaggacg cccgacgtta aagaacgcca
    48481 tcagttcgcg ttcagacggt aattgttcac cttcgccaaa ttcacgacgg cggatcatct
    48541 gttccagctc ttcttccacc atttcggaga gttttttacg cgccagcggg cggctacgca
    48601 agttgcgacc aattgcaggt gaagaatctt cggtttgcga atcaaatgcg ttcataaggc
    48661 ccattctgta aggtcagtgt gattaacatc atcagtgaca tcctatcaca ggattgaaag
    48721 taggggaaaa tggcaggttt ttctctttgt gcctcatcat taccataatt aacggaataa
    48781 ttaactattg cgaaaaatta atgtaacgca gataaacaca tcccgattga attattcata
    48841 agactattca caagcattat gaatattatg aatgtgttct tacaaaataa tcataagcgc
    48901 atatttttta atgaaaaatc acatcaccta caattaaaaa cacgacatcc gcaccataaa
    48961 tagacttgca aaaaatataa catcattgtt ttcaatctgc cgtttatggg attgaccgtt
    49021 ttcttttgac acggagttca acaatgttcg gcataattat atctgtcatc gtattaatta
    49081 cgatgggcta tttgatcctg aaaaactaca aacctcaggt ggtgctggct gccgcaggtg
    49141 tcttcctgat gatgtgcgga gtctggttag ggttcggtgg tgtactcgat cccgccaaaa
    49201 gcagcggcta cttgatcgtc gacatttata atgaaatcct gcgcatgctg tccaaccgca
    49261 ttgccggatt ggggctgtcg attatggcgg tgggcggtta tgcccgctac atggagcgca
    49321 taggagccag tcgcgcgatg gtgagcttgc taagccgccc gttaaaactc attcgctcgc
    49381 cgtatattat tctgtcggca acttacgtca tcggccaaat catggcgcag tttattacca
    49441 gcgcctccgg tctgggtatg ttgctgatgg tcaccttatt tccgacgctg gtgagtctgg
    49501 gagtaagtcg cctctctgcg gtggcggtta tcgcaaccac gatgtccatt gagtggggga
    49561 ttctggaaac gaactccatt tttgcagccc aggtcgcggg aatgaaaatt gccacatact
    49621 tcttccacta ccagcttccg gtcgcctctt gcgtcattat ctcggtggcg atctcccact
    49681 ttttcgtgca acgcgctttt gacaaaaaag ataaaaatat caatcacgaa caggcagagc
    49741 aaaaaactct cgataatgtc ccgccgctct attacgccat tttacctgtg atgccgttaa
    49801 tcctgatgct cggctcgctg ttcctcgccc acgtcgggct gatgaagtca gaactgcatc
    49861 tggtggtggt gatgttacta agtttgacgg tgacgatgtt tgttgagttc ttccgcaagc
    49921 ataacttgcg cgaaacaatg gacgatgtgc aggcgttttt tgacggcatg ggtacgcagt
    49981 ttgccaacgt ggtaacgctg gtggtcgcgg gtgaaatatt tgcgaaaggc ttaacgacga
    50041 ttggcactgt tgatgcggtt atcaggggtg cggagcattc tggtctgggc ggtattggcg
    50101 tgatgattat tatggcgcta gtcattgcca tttgtgccat tgtgatgggc tctggcaatg
    50161 cgccgtttat gtcatttgcc agtcttattc cgaatatcgc agccggacta catgtaccag
    50221 cggttgtaat gattatgccg atgcattttg ccacgacgct agcgcgcgcg gtttcgccga
    50281 ttactgcggt ggtgaagcgg acagcgatcc ccatggcagt cggtttcgtg gtgaatatga
    50341 ttgccacaat cacgctattt tattaagtca ttaaaaagac aaaacaggcc gcctgggcct
    50401 gttttgtatt acttcacaac acgtaatgcc ggtcgaccac cgcgtggtgg ctgcggaggt
    50461 tcatcgtcag gatgagtgtc atcatcgtga tctggcttgt cgccatcaat aaccgacata
    50521 acggtttcgt tgtctgccga tgcctcttca tcattcatga tgctggtatc ttcatcgtag
    50581 gcagcttcag gctcaaacat cgtgcctgcg ccattttcac gggcgtagat agccagcacg
    50641 gcagccagcg gcacagaaac ctgacgcgga atgccaccaa agcgcgcgtt aaagcgcacc
    50701 tcatcattcg ccagttccag attgccgaca gcacgcggcg caatgttgag tacgatttgc
    50761 ccgtcacgcg catattccat aggaacctgc acgccaggga gcgtcacatc caccaccagg
    50821 tgcggcgtga gctggttatc cagcaaccac tcatagaatg cacgcagcag atagggacga
    50881 cgtggtgtta gctgtgacaa atccatacag attaactccg gcccagacgc atttcacgtt
    50941 ctgcttcagt taaagaagca aggaaagagt cacgctcaaa gacgcgggtc atatagcctt
    51001 tcagctcttt cgcacccggg ccgctgagct cgatacccag ttgcggcaga cgccacagca
    51061 gcggagcaag atagcaatcg accaggctga actcatcgct caggaagtac ggcttctgac
    51121 cgaagaccgg cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg gcatctgctt
    51181 cagaagctga accgttgatg atggtgttca tcagcgtgta ccagtctttt tcgatgcgat
    51241 gcatgtacag acggctttca ccgcgagcta ccgggtaaac aggcatcagt ggcggatgcg
    51301 ggaaacgctc atccagatat tccataatga tgcgagattc ccacagggtc agctcacgat
    51361 ccaccagggt cggaacgctc tgattcgggt tgaggtcaat cagatcctga ggcggattgt
    51421 ccttttccac gtgttcgatc tcgaaactta cacctttctc agccagcaca atgcggacct
    51481 gatggctata gatgtcagta ggaccggaaa acagcgtcat taccgaacgt ttgttggcag
    51541 cgacagccat gaaaacctcc aggtatagtc agaattttta ctgctaccag ccaccaggtg
    51601 gccagtcaga agttgtgtta cccaataagg aacgactctc tttgttcgaa aatcaaacaa
    51661 aaaatgagca atacccgaca tttgggcaga aaattggatg atagtttacc agattttgcg
    51721 accattgtgg tgagtggatg ccggaaatgg ggaaaaagag atgcgcttta gtctgaaata
    51781 gttgacttag acccttattg gcgatgtggt ttttgttttg cctgtctttc agacggcagc
    51841 aaaaagcaac ttcccatttt ttacgctgat tcagaattta gccataaaaa aacccgccga
    51901 agcgggtttt ttcgtaaatt gttttctgcc ggagcagaag ccaattaacg tttggagaac
    51961 tgcggacgac gacgtgcttt acgcagaccg actttcttac gttcaacctg acgagcgtca
    52021 cgagtaacga agccagcttt acgcagttca gaacgcaggg actcgtcgta ttccatcaga
    52081 gcgcgggtga taccgtgacg gatcgcacca gcctgaccag agataccacc acctttaacg
    52141 gtgatgtaca ggtccagttt ctcaaccatg tcgaccagtt ccagcggctg acgaactacc
    52201 atgcgggcag tttcacgacc gaagtactgt tccagagaac gttggttgat tacgatttta
    52261 ccgttgcccg gtttgatgaa aacgcgagct gcggaatttt tgcggcgacc agtgccgtag
    52321 tattgatttt cagccattgc ctataatccc gattagatgt caagaacttg cggttgctgt
    52381 gccgcgtggt tgtgctcgtt acccgcgtaa actttcagtt tacggaacat agcacgaccc
    52441 agcgggcctt ttggcaacat gcctttaacc gcgatttcaa tcacacgctc aggacggcga
    52501 gcaatcatct cttcaaagct cacttgtttg ataccaccga tgtggccggt gtggtgatag
    52561 tacactttgt cagtacgctt gttgccggtt acagcaactt tgtcagcgtt cagaacgatg
    52621 atgtaatcac cggtatctac gtgcggagtg tattccgctt tgtgcttacc gcgtaggcga
    52681 cgagccagtt cagtagccag acggcccaga gttttaccgg tcgcgtcaac aacataccag
    52741 tcgcgtttta cggtttctgg tttagctgta aaagttttca ttaaaagctt acccaataaa
    52801 tagttacacg ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc
    52861 cggcaaacct accccttcga atagcctatg ccagcacaca aaaagttttg ggaaaaaaac
    52921 tttcttgtaa cgtggggtcg caggattata gagaagtcgg ggtcaaagat cgaccccttt
    52981 ttgtgatttg tgacaggttt taacccgcca aatgctcgcg cttcagatac tcttcgcttt
    53041 gcatctcttg cagacgtgac aggcaacgct ggaactcaaa cttcagccga tcgccctgat
    53101 aaatttcata cagcggcact tctgcactca ccactaattt gacatggcgc tcgtaaaact
    53161 catccaccag cgcaataaag cgccgcgctt cgctctccat caaccgcgtc ataactggta
    53221 catcaaacaa catgaccgta tgaaagagac gtgagagcgc aatatagtca tgctgactgc
    53281 gggcgtcgac gcacagcgta gtaaaagaga ccgccagcgt ctggttctcg acgcccattg
    53341 tcgctaatgg ccgatggttg atttctaacg tcggtgaatt ttctcgtttc ccccccgcca
    53401 gcgccaacca tagtttatcc atttgcgccc gggtttcatc gtggagtggc gaaagccaca
    53461 gatgcgcctg agtgagtgta cgcagtcgat aatcaacacc agcgtccacg ttcattacat
    53521 cacaatgctg tttaatggca tcgattgcag gcagaaaacg cgcacgttgc aagccatttc
    53581 gataaagttc gtccggcgga atatttgacg tcgctaccag ggtaataccg cgggcgaaca
    53641 aggctttcat cagaccgcca agtagcatgg catcggtaat atcagaaaca aaaaattcgt
    53701 caaaacagag cacgtcagtt tcggctttaa agcgatcggc aataatttcc agcggatcgg
    53761 tctgcccctg taaggcagtt agctcttcat gcacccgcag cataaaacgg tgaaagtgca
    53821 ggcgctgttt ccgctctccc ggcaggcttt gatagaaaag gtccatcagc caggttttcc
    53881 cgcgtcctac accgccccac atatataagc cacgcactgg cgtatgcttt gtgtcttcgc
    53941 gtttacccca cagcttaccg acccgcgcca ttagcccact cgtcctgggg gctggtggcg
    54001 tgctattgat gagttcctga taaataattt ccaggcggct gacggcctct ttttgaacgt
    54061 cgtcgggttg atggctgcct tcattgagcg ccttcaggta ttgcgatgtt ggggtaacgc
    54121 tttgcatgat cttattgtta ttccttgaat aatcggtgcg ccgttgttca cggttgacga
    54181 aaaaaaggcc gttctacact acgcgatatg cagtcgggat tccacttctg tggaattaac
    54241 ggttatagtg gcataatcag ccgcaggcat ggagcctgaa gccaacaccc tacggaaaca
    54301 aaagacaacg ggagatgttc atgacctggg aatatgcgct aattgggtta gtcgtcggca
    54361 tcattattgg tgctgtggcc atgcgttttg gtaatcgtaa actacgccag caacaggcgt
    54421 tgcagtacga actggaaaag aataaagctg aactggacga gtatcgcgaa gagctggtta
    54481 gccactttgc ccgcagcgcg gaattactgg ataccatggc gcacgactat cgccagctgt
    54541 atcagcacat ggcgaaaagt tccagcagcc tgctgccgga actgtctgct gaagcaaacc
    54601 cgttccgtaa tcgtctggca gagtctgaag ccagcaacga tcaggcaccg gtgcagatgc
    54661 ctcgcgacta ttctgaaggc gcatccggcc tgctgcgtac tggcgcgaag cgcgactaat
    54721 ttatttttcg ggcgcagcca ttgcgccctc ctcttctctc cctccccgac tatcatttaa
    54781 tctggtgtct cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt ttgaatctct
    54841 tttcttatca ttcaggtacg agagcaggaa taatgaaaaa acaaccccag ctgttgagtg
    54901 cattagcgtt aagtgtcggg ttaactctct cggcgtcatt tcaggccgtc gcgtcgattc
    54961 caggccaggt tgccgatcag gcccctctcc ccagtctggc tccaatgctg gaaaaagtgc
    55021 ttccggcagt ggtgagcgta cgggtggaag gaacggccag tcagggacag aaaatcccgg
    55081 aagaattcaa aaagtttttt ggtgatgatt taccggagca acctgcacaa cccttcgaag
    55141 gtttaggctc cggtgtcatc atcaacgcca gtaaaggcta tgtgctgacc aacaaccatg
    55201 tgattaatca ggcacagaaa atcagtattc agctcaatga tgggcgcgag tttgatgcaa
    55261 aactgattgg tagcgatgac cagagcgata tcgccctgtt acaaattcaa aacccgagca
    55321 aattaacgca aatcgctatt gccgactccg ataaattgcg cgtcggtgat tttgccgtag
    55381 cggtcggtaa cccatttggc cttgggcaaa ccgccacctc tggcattgtt tccgcattag
    55441 gacgcagcgg gttgaatctt gaaggtctgg aaaactttat ccagacagat gcttccatta
    55501 accgcggtaa ctccggcggt gcactgttaa accttaacgg tgagttaatt ggcatcaaca
    55561 ctgcaatcct tgcgcctggc ggcgggagcg tcgggattgg atttgccatc cccagtaata
    55621 tggcgcgaac actggcgcag cagcttatcg actttggtga aatcaaacgc ggtttgttag
    55681 gcatcaaagg caccgagatg agtgccgata tcgccaaagc cttcaacctt gacgtgcagc
    55741 gtggcgcgtt tgtcagcgaa gtgttgccag gttctggctc ggcgaaagcg ggcgtcaaag
    55801 cgggcgatat tattaccagc ctcaacggca aaccgctgaa tagctttgct gagttgcgct
    55861 ctcgtatcgc gaccaccgag ccgggcacaa aagtgaaact tggcctgctg cgtaacggca
    55921 aaccactgga agtagaagtg acgctcgata ccagcacctc ttcgtcggcc agcgctgaaa
    55981 tgatcgcgcc agcgctggaa ggtgcaacgt tgagcgatgg tcagctaaaa gatggcggca
    56041 aaggtattaa gatcgatgaa gttgtcaaag gaagcccagc tgctcaggct ggcttgcaaa
    56101 aagacgatgt gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct gaaatgcgta
    56161 aagtgctggc ggcaaaatcg gccatcatcg ccctgcaaat tgtacgcggc aatgaaagca
    56221 tctatctgct gatgcgttaa tgtcgtaaac cgggcatcag gcttacgtgt gatgtccggt
    56281 taactcgtgg tatgctgctg ccgttccctt ttttaatgac gcctccatca tgtttgtgaa
    56341 gctcttacgt tccgttgcga ttggattaat tgtcggcgct attctgctgg ttgccatgcc
    56401 ttcgctgcgc agccttaacc cgctttccac tccgcaattt gacagtaccg atgagacgcc
    56461 tgccagctat aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg tttacaaccg
    56521 tggtttgaac accaactctc acaaccagct tgagatccgc accctgggat ccggtgtaat
    56581 catggatcaa cgcggttata tcatcaccaa taaacacgtc atcaacgacg ccgatcagat
    56641 catcgtcgcc ttacaggatg gtcgtgtatt tgaagcattg ctggtgggat ctgactctct
    56701 aaccgatctg gcggtactta aaattaatgc cactggcggt ttacctacca ttccaatcaa
    56761 tgcacgtcgc gtaccgcata ttggcgacgt ggtactggcg atcggtaacc cgtacaacct
    56821 cgggcagacc attacccagg ggattattag tgccacgggt cgaattggtc tgaacccgac
    56881 cgggcggcaa aacttcctgc aaaccgatgc ttccattaac cacggtaact ctggcggcgc
    56941 gctggtgaac tcgctgggcg aactgatggg cattaacacg ctgtcgtttg ataagagtaa
    57001 cgatggcgaa acgccggaag gtatcggctt tgcgattcct ttccagttag caaccaaaat
    57061 tatggataag ctgatccgcg atggtcgcgt gatccgcggc tacattggca ttggcggacg
    57121 tgagatcgca ccgctgcacg cacagggcgg tggtatagat caactgcaag ggatcgtggt
    57181 taatgaagtg tcacctgacg gcccggcggc gaatgcgggt attcaggtca acgatctgat
    57241 tatttcggtg gataacaaac cggccatctc tgctctggag acgatggatc aggtggcgga
    57301 aattcgccct ggttcggtga tcccggttgt agtgatgcgt gatgataagc agttaacgct
    57361 gcaggtcacc attcaggaat atccggcaac caattaagtc gagtgctcaa aacaaaaaac
    57421 cggagtctgt g
//
LOCUS       DAJTYZ010000011        55736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000011 DAJTYZ010000000
VERSION     DAJTYZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.011"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="OO596_000657"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="OO596_000657"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6716605.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OO596_000658"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OO596_000658"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6716606.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OO596_000659"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OO596_000659"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6716607.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OO596_000660"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OO596_000660"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6716608.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OO596_000661"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OO596_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6716609.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OO596_000662"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OO596_000662"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6716610.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="OO596_000663"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="OO596_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6716611.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OO596_000664"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OO596_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6716612.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="OO596_000665"
     CDS             6537..6773
                     /locus_tag="OO596_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6716613.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="OO596_000666"
     CDS             6776..7633
                     /locus_tag="OO596_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6716614.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="OO596_000667"
     CDS             7654..9645
                     /locus_tag="OO596_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6716615.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OO596_000668"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OO596_000668"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6716616.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="OO596_000669"
     CDS             complement(10248..11144)
                     /locus_tag="OO596_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6716617.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="OO596_000670"
     CDS             complement(11193..11432)
                     /locus_tag="OO596_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6716618.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="OO596_000671"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="OO596_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6716619.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="OO596_000672"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="OO596_000672"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6716620.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="OO596_000673"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="OO596_000673"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6716621.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="OO596_000674"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="OO596_000674"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6716622.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="OO596_000675"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="OO596_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OO596_000676"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OO596_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6716623.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="OO596_000677"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="OO596_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6716624.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="OO596_000678"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="OO596_000678"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6716625.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="OO596_000679"
     CDS             complement(21570..22739)
                     /locus_tag="OO596_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6716626.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OO596_000680"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OO596_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6716627.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="OO596_000681"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="OO596_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6716628.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="OO596_000682"
     CDS             complement(24315..25247)
                     /locus_tag="OO596_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6716629.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="OO596_000683"
     CDS             25360..26571
                     /locus_tag="OO596_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6716630.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="OO596_000684"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="OO596_000684"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6716631.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OO596_000685"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OO596_000685"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6716632.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="OO596_000686"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="OO596_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6716633.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="OO596_000687"
     CDS             complement(30320..31576)
                     /locus_tag="OO596_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6716634.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="OO596_000688"
     tRNA            31884..31960
                     /locus_tag="OO596_000688"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="OO596_000689"
     tRNA            31965..32041
                     /locus_tag="OO596_000689"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="OO596_000690"
     CDS             32149..32454
                     /locus_tag="OO596_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6716635.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="OO596_000691"
     CDS             32580..34184
                     /locus_tag="OO596_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR6716636.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /locus_tag="OO596_000692"
     CDS             complement(34196..35008)
                     /locus_tag="OO596_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716637.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /locus_tag="OO596_000693"
     CDS             complement(35012..35797)
                     /locus_tag="OO596_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6716638.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="OO596_000694"
     CDS             complement(35794..36462)
                     /locus_tag="OO596_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6716639.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="OO596_000695"
     CDS             complement(36526..37164)
                     /locus_tag="OO596_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6716640.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39285)
                     /locus_tag="OO596_000696"
     CDS             complement(37177..39285)
                     /locus_tag="OO596_000696"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR6716641.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGL
                     PLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPM
                     TVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDM
                     RNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETL
                     QRLGLEDIAADLAAHNLLPA"
     gene            complement(39306..39932)
                     /locus_tag="OO596_000697"
     CDS             complement(39306..39932)
                     /locus_tag="OO596_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6716642.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(40388..40597)
                     /gene="fumD"
                     /locus_tag="OO596_000698"
     CDS             complement(40388..40597)
                     /gene="fumD"
                     /locus_tag="OO596_000698"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6716643.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            41154..42566
                     /gene="pykF"
                     /locus_tag="OO596_000699"
     CDS             41154..42566
                     /gene="pykF"
                     /locus_tag="OO596_000699"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6716644.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            42876..43112
                     /gene="lpp"
                     /locus_tag="OO596_000700"
     CDS             42876..43112
                     /gene="lpp"
                     /locus_tag="OO596_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6716645.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(43175..44179)
                     /gene="ldtE"
                     /locus_tag="OO596_000701"
     CDS             complement(43175..44179)
                     /gene="ldtE"
                     /locus_tag="OO596_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6716646.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(44328..44744)
                     /gene="sufE"
                     /locus_tag="OO596_000702"
     CDS             complement(44328..44744)
                     /gene="sufE"
                     /locus_tag="OO596_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6716647.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(44757..45977)
                     /gene="sufS"
                     /locus_tag="OO596_000703"
     CDS             complement(44757..45977)
                     /gene="sufS"
                     /locus_tag="OO596_000703"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6716648.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(45974..47245)
                     /gene="sufD"
                     /locus_tag="OO596_000704"
     CDS             complement(45974..47245)
                     /gene="sufD"
                     /locus_tag="OO596_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6716649.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(47220..47966)
                     /gene="sufC"
                     /locus_tag="OO596_000705"
     CDS             complement(47220..47966)
                     /gene="sufC"
                     /locus_tag="OO596_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6716650.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(47976..49463)
                     /gene="sufB"
                     /locus_tag="OO596_000706"
     CDS             complement(47976..49463)
                     /gene="sufB"
                     /locus_tag="OO596_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6716651.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(49472..49840)
                     /gene="sufA"
                     /locus_tag="OO596_000707"
     CDS             complement(49472..49840)
                     /gene="sufA"
                     /locus_tag="OO596_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6716652.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(50388..50576)
                     /locus_tag="OO596_000708"
     CDS             complement(50388..50576)
                     /locus_tag="OO596_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6716653.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(50676..51086)
                     /gene="menI"
                     /locus_tag="OO596_000709"
     CDS             complement(50676..51086)
                     /gene="menI"
                     /locus_tag="OO596_000709"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6716654.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(51083..54139)
                     /locus_tag="OO596_000710"
     CDS             complement(51083..54139)
                     /locus_tag="OO596_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6716655.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            54528..55640
                     /gene="ydiK"
                     /locus_tag="OO596_000711"
     CDS             54528..55640
                     /gene="ydiK"
                     /locus_tag="OO596_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6716656.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT        13420 a        14655 c        13639 g        14022 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 agatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatt ttaatgaagt taacgtcacc
    38041 cgcttccagt tgatcccagc gaatttccgc atactcttca gcaggcagaa cacgcttgaa
    38101 cacacctttg ctgtaacaat aggtaaagtc gcgatgcaac tgcccgtagt tacaccatag
    38161 gccgtagtca tcgaacagat tcagacccac caggttacca atcacacgac catcgtcttt
    38221 atcttcaaaa tctttcggac cgttcgggaa gatggtggta tggacaaagt tggctacaca
    38281 agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat tcatttgggt
    38341 catacaacgg atcgggcaag agtggcagcc actcattttt actgtgtatt tctctgccgc
    38401 cggacctaag tcaaaaacgg atttataggt acgaaagccg accgtgttct gattgcctgg
    38461 cggaatttcc cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca gccctttacg
    38521 tgctgtccaa cgtgacttgg gatctgaata ctctgcccac gattgcggcg tacttggcac
    38581 gacatggttg ttattcgcac caataagttc agtcatcatg taatcgttga gacgcttcat
    38641 ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg ctttcaggtt
    38701 tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc tattcaacat
    38761 gccagagaga ggaacaaggt tttccccagc ctgaccaata gccgccacac aggtttccgg
    38821 actggtcaat cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc
    38881 ggctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg atttcgcctt
    38941 cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc
    39001 catatgggca tcgacgacta aatttccttt ggtaaaagta gaaagtgagg tgatatttac
    39061 gcgagaacta cagggggcac cagatccagt taatgggccg gtagcaaaga ctaatttatt
    39121 cgcttcatcg aaaggtttcg tgcctggcgg tacttcgtca tacataattt tgtagccgaa
    39181 gcccatgcca ccgacaaaac ttttaaactt actggaatct tcgagggtaa tatttcctgt
    39241 cgtgagattg actcttaata tattacctgt ccaaccgtta gccatgattt tttcctttgc
    39301 aagattcaca cagtaatatc tttccactcg ataattttta acgcccctgt tgggcaggcg
    39361 tttgcgcatt caccgcataa cacacatttc gaggattttt tactttcggt atttacggtg
    39421 gccatcatcc acgggcatgc cgtagtacag gcgctacagc caatacagcg tttatgatcg
    39481 acggtaatac agccttcttt ctgctgccag gtaatcgcac caatcgggca gacgttcatg
    39541 cattgcggct ctttgcattg acggcaggtg tccgcggtat agttgagatc gccatacagg
    39601 ccgccgccag agccaacccc gttgtcgcca aagaaataat tgcgatggat tttgatacgg
    39661 gagaagaatg tccctactga gccatcgttg aagttggtac aagagatttc acagcggtgg
    39721 caacccgtgc atcgcgctcg ctgggtcacc agcacccctt tgggtgtatt tatcaaccct
    39781 actgtgccgc tatcaatatc ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca
    39841 attgttagac cagcaaggcc ttttcctgat atacgcaaga attctaatcg cgttaagcca
    39901 atatctaata gtggacgatc aaccgggttc atttatttcg catcctcttc gcaaacacag
    39961 tgctgacaat tcagcagcct gatattaagc attcaactaa tacgctgctg actctgtccc
    40021 caacaccaga tattttccct aacccgatag tgtatgagaa aagatttaac gccgataaac
    40081 caatccttta tggttaggag aaaaaataat gtgattattc tgagccctta acattgatcg
    40141 ttatcaatta aaattacaaa gcaagaaatt ataagtgaac tgatatttat tatcaattga
    40201 aataaattta acttaagcga caattatttg tgacgttcat tgcagaaaat aaaaataata
    40261 ttatcgttat agattaaaat acataccgca attcgtataa tttcagcagt tgttatttag
    40321 aatgattaac tggaccgcaa ctgaagcaag aatatcagga tgaagcttac cgcctcatcc
    40381 tgaatggtca ttttaccagt gcgttaatga cggtttccat cgcctgcttt tccagctcac
    40441 tgcgctgaat gcgtttattg gctaatgcag tacgtaatac gcctgcgata acaatatgct
    40501 ttggttcctg ccccagatcg cgcatttcca gcacaacttt accgacaact ctgcacattt
    40561 ctcggtacag ttcatcctct tttgtccgat ttcccattct ttatacctat ttatcatgcc
    40621 aactatcagc atatatcaat ctaaccaatt aaacaaaaat caaacaaaat cagacaaata
    40681 acgcgataaa ttattttaac tgtagcaatt gagcgatgat atatttatac accggatgaa
    40741 ctttcactta tcctcacact gacaacttcg gcaccagacg ttgcgcaaac agcgaagttt
    40801 ttgcgtaacc ttttccctgg aacgttaaat ctttgataac aatttattgt ctaacaagtt
    40861 gtatattttt tgaaacgctg tttttgtttt ccttttggat taatttcagc gtataatgcg
    40921 cgccaattga ctcttgaatg gtttcagcac tttggactgt agaactcaac gactcaaaaa
    40981 caggcactca cgttgggctg agacacaagc acacattcct ctgcacgctt tttcgatgtc
    41041 acctatcctt agagcaaggc accaccactt tcgtaatacc ggattcgctt tccggcagtg
    41101 cgcccagaaa gcaagtttct cccatccttc tcaacttaaa gactaagact gtcatgaaaa
    41161 agaccaaaat tgtttgcacc atcggaccga aaaccgaatc tgaagagatg ttagctaaaa
    41221 tgctggacgc tggcatgaac gttatgcgtc tgaacttctc tcatggtgac tatgcagaac
    41281 acggtcagcg cattcagaat ctgcgcaacg tgatgagcaa aactggtaaa accgccgcta
    41341 tcctgcttga taccaaaggt ccggaaatcc gcaccatgaa actggaaggc ggtaacgacg
    41401 tttctctgaa agcgggtcag acctttactt tcaccactga taaatctgtt atcggcaaca
    41461 gcgaaatggt tgcggtaacg tatgaaggtt tcactactga cctgtctgtt ggcaacaccg
    41521 tactggttga cgatggtctg atcggtatgg aagttactgc cattgaaggt aacaaagtta
    41581 tctgtaaagt gctgaacaac ggcgacctgg gcgaaaacaa aggtgtgaac ctgcctggcg
    41641 tttccattgc cctgccagca ctggctgaaa aagacaaaca ggacctgatc tttggttgcg
    41701 aacaaggcgt agactttgtt gctgcttcct ttattcgtaa gcgttctgac gttatcgaaa
    41761 tccgtgagca cctgaaagcg cacggcggcg aaaacatcca catcatctcc aaaatcgaaa
    41821 accaggaagg cctcaacaac ttcgacgaaa tcctcgaagc ctctgacggc atcatggttg
    41881 cgcgtggcga cctgggtgta gaaattccgg tagaagaagt tatcttcgcc cagaagatga
    41941 tgatcgaaaa atgtatccgt gcacgtaaag tcgttatcac tgcgacccag atgctggatt
    42001 ccatgatcaa aaacccacgc ccgactcgcg cagaagccgg tgacgttgca aacgccatcc
    42061 tcgacggtac tgacgcagtg atgctgtctg gtgaatccgc aaaaggtaaa tacccgctgg
    42121 aagcggtttc tatcatggcg accatctgcg aacgtaccga ccgcgtgatg aacagccgtc
    42181 tcgagttcaa caatgacaac cgtaaactgc gcattaccga agcggtatgc cgtggtgccg
    42241 ttgaaactgc tgaaaaactg gatgctccgc tgatcgtggt tgctacccag ggcggtaaat
    42301 ctgctcgcgc agtacgtaaa tacttcccgg atgccaccat actggcactg accaccaacg
    42361 aaaaaacggc tcatcagttg gtactgagca aaggcgttgt gccgcagctg gttaaagaga
    42421 tcacttctac tgatgatttc taccgtctgg gtaaagaact ggctctgcag agcggtctgg
    42481 cacacaaagg tgacgtcgta gttatggttt ctggtgcact ggtaccgagc ggcactacta
    42541 acaccgcatc tgttcacgtc ctgtaatatt gcttttgtga attaatttgt atatcgaagc
    42601 gccctgatgg gcgctttttt atttaatcga taaccagaag caataaaaaa tcaaatcgga
    42661 tttcactata taatctcact ttatctaaga tgaatccgat ggaagcatcc tgttttctct
    42721 cagttttttt atctaaaacc cagcgttcga tgcttctttg agcgaacgat caaaaataag
    42781 tgccttccca tcaaaaaaat attctcaaca taaaaaactt tgtgtaatac ttgtaacgct
    42841 acatggagat taactcaatc tagagggtat taataatgaa agctactaaa ctggtactgg
    42901 gcgcggtaat cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc
    42961 agctgtcttc tgacgttcag actctgaacg ctaaagttga ccagctgagc aacgacgtga
    43021 acgcaatgcg ttccgacgtt caggctgcta aagatgacgc agctcgtgct aaccagcgtc
    43081 tggacaacat ggctactaaa taccgcaagt aatagtacct gtgaagtgaa aaatggcgca
    43141 cattgtgcgc catttttttg tctgctattt accactactg cgtcgcgcgt aacatattcc
    43201 cttgctctgg ttcaccattc tgcgctgact ctactgaagg cgcattgctg gctgcgggag
    43261 ttgctccact gctcaccgaa accggatacc ctgcccgacg atacaacgct ttatcgacta
    43321 acttctgatc tacagcctta ttgtctttaa attgcgtaaa gcctgctggc agtgtgtatg
    43381 gcattgtctg aacgttttgc tgttcttctg ctgatagtgg tcgatgtact tcaacataac
    43441 gcatcccgtt aggttccacg gaatatttca ccggttcgtt gatcactttc accggcgttc
    43501 ccgtccgcac gctggagaac aaggctttaa tatccggtgc attcatgcga atacaccctg
    43561 aactgacgcg caaaccaacg ctgtccggcg cactggtgcc atgaatgagg tattcgccat
    43621 taccatgcgc gaggcgcagt gcgtaacgtc ctagcgggtt atttggtccg gcaggaacga
    43681 ctggcggtaa tttaatgcca cgctccagcg aacgctgacg aatgcctgcc gtaggcgtcc
    43741 aggttgggtt agggattttc tgcccaacac gcgtttccat caccggcgtt tccagcccct
    43801 gcaatccaat acctattgga taaacctgca caatattttc tcccggcgga taataataaa
    43861 ggcgcagctc tgcaaggtta acgataatcc cctgacgcgg tgcatcaggt aataacagtt
    43921 gtgaaggaat agttatcgtc gtaccaggtt ttggcaccgg gacgatagtg ttattggctt
    43981 caaggatcaa cattgccgca gtatcaaaac gtcgggcaat agcctgaagg tttttatccc
    44041 cttcttgcac cgtatacgtt tgattttgcc caaccagtcg gcttccggtt ggtggtagcg
    44101 gataatcaac cgcccaggca gcctggatgg cgctaaaagc gccgataagc gtgagtgtaa
    44161 gcaaagacgc gcgtttcatt gtaaacctcc tgtatttgcc ggagactcac gctgaaacgt
    44221 cggatggcgc ttatgttcac ctgaaaccaa aacactcctg tgcaggtcag tgtaaacatt
    44281 gaccatccgg caatgtgagc caaccggatg aaagctgtcc ctttagttta gctaagtgca
    44341 gcggctttgg cgcgaattgc gcgaatcatc gcttccagac cttgtgaacg agatggggtg
    44401 agatgttggg tgagtgccat tttttcaaac cacggacgca catcgaaact gacaatatcc
    44461 tgcggcgtca tctgatcgta gagaataaag actaccgcaa taagcccttt cacaatcgcc
    44521 gcatcgctgt cgccctgcaa ttcaataatt ccgtgcgcat tctggcgcat gacaatccac
    44581 acctgactct ggcagccctg aatgctattt tgtgggcttc ggtcttcgtc gcgtaattca
    44641 gtcagacgct ggcccagctc aataatgtag agatatttct cttcccagtt ggcgcagcgt
    44701 aaaaaattac gcagcaattt ttctttatcc ggcaataaag ccatagtgcc tccctgttat
    44761 cccagcaaac ggtgaatacg ttgcaggccg gtcaccagac gatccacttc ttcatgggtg
    44821 ttatacatgg ccagcgacgc ccgacacatc gcagggacgt tgtaataggc cattaatggc
    44881 atagcgcagt gatgtccggt acgcacagca atgccgtaat tatcgagaaa actgccaaca
    44941 tcataggcgt ggtgtttacc gagattaaaa gcaataacgc caagcctgtt ttgtgggcca
    45001 tagagagtga gatccggtac agattccagc tgtgatagcg cataatgcat cagattctgt
    45061 tcatactcgg ctatgttatt aagccccagc gccgaaacat actccagcgc cgcgccaaga
    45121 ccaataatgc ccccggtatt gggtgtaccg gcttcaaacc gccatggtgc tttggtccag
    45181 gtagtgcctt cactcaggct gacggtggcg atcatagaac cgcccccttc ccacggcggc
    45241 atctcctgca acaaggcttc tttcacataa agaatgccaa ttccggtggg gccatacagt
    45301 ttatgcccgg agaacacgta aaagtcgcaa tccagcgcct gaacatccac cggatgatgc
    45361 atcaccgcct gagcgccatc caccagcact tttgcgccat gctggtgcgc aagcgtgatt
    45421 atttccgcca gcggattttc tgttcccagc acgttggaga catgagtaat tgccagcagg
    45481 cgagttttct catcaaacag cgtaggcagc gtctccagtt gcagcgtacc gtcggggttg
    45541 agagggatca cacgcagctc tgcgccaacg cgtgcgcaaa gcatctgcca gggaacaatg
    45601 ttagcgtggt gctccatctg actgatgatg atgttatcgc ccgcccgcac gttgctgttg
    45661 ccccaactat tggcgaccag attgatccct tccgtcgtgc cgcggacgaa caccagctct
    45721 tccgccgaac gggcattaat aaacagcgat gcccgcttgc gcacgttctc cattttctcg
    45781 gtcgcctggg cgcttaaggt atgaataccg cgatgcaccg ccgcgtagcc gtgacgataa
    45841 aactcagcct cggcgtcaat cacctggctt ggtttctgcg cactggcggc gctatcgaga
    45901 taagccagcg gcaaaccgtt cacctcacga gaaagcaccg gaaagtcggc ccgcactttg
    45961 tcgacggaaa aagtcatctt gcacctcctg gcagccgttg accgattcgg gccagcacct
    46021 gctgtttaag cccctcatca cgcagtgctt ccgtcagttc ggcagcaaag gcgtaaatga
    46081 tcatctgctg ggcatcctgc tgattgatcc cgcgcgagcg cagatagaac atctgttcat
    46141 catcaatacg ccccaccgtc gcgccgtggc tgcatttcac atcatctgca tagatttcca
    46201 gctgcggttt tgtatccact tccgccattt tgcccatcag cagattattg ttggtcatct
    46261 gaccatccgt tttgatggcg tgctgcgcga cgttgatcaa accgttaaat accgcgcggc
    46321 ctttgtcgct gacgatagtt ttgtgcaact gtcggctgtt acaaaaacct ttattgtgtt
    46381 ccagccaggt acgggtatca cacacctcgt ttttcaccgg catcgccagg ctattgatcc
    46441 gcagcgtgct gttttcgcca ttgagttgcg tactggtgtt gtgtcgtaac actgcgccac
    46501 ccagcaggaa actatggcta aatgcggtgg catcctcagc cagcaacaaa tcgttatgag
    46561 caaagtggtg actgagcggg ttttcaaacg ccagcttgat atgctgcaag tgggcgttcg
    46621 ctgcgacatt gatagtgaac cgtgccccgg taaaatgacg agcatcatta aggctgacaa
    46681 aatgttcgat caccgttgct tcggcacctt ccgccagatc cagatgatgc cggtaatggg
    46741 cagtgttcac ctcttcacct gccacgccct gggtgatatg cattaacagc aatggctttg
    46801 ccggccgttg accgcgcttc acggcgatat gcgtcacgct ttgtgccagg ctttccgtca
    46861 aatgcagaaa cacttccgcc tgaatagcgt cgggtaaccc ctgacggtcg tcgttaatgc
    46921 tcacttcata tccgctgcct tcaattgcat cgctcagcgc gggcacgtaa cgtccatcga
    46981 caaacaccag ccgcacggcg tctaacgtta acgctaaggc atcacgctgc tgtggggata
    47041 tatctcccgc aatgctgaca aactgactat tggtcagccc ttccagcggc gtatatttcc
    47101 agttttcatg tttacgtgtc ggcagtccgg tacgcagcaa ttgttgtaaa tgctgctgtg
    47161 cttgcggtga gcgttttgtc ccttcagctt caaacaagtg atgccactgt tgcagcgcat
    47221 tactgctgtt cggtaagcca gccataaccc tgctcctcca gttgtttgac caacgtgaaa
    47281 tcgccggatt tcacaattcg tccctgatat agcacatgga cgtaatcagg cttgatgtag
    47341 tcgagaatgc gttggtagtg cgtaacaatg atgaatgagc gcttgccgtc acgcagcgag
    47401 ttcacgccat cggcaaccac ttttaatgcg tcaatatcca gcccggagtc cgattcatca
    47461 agaatgcata actccggttc cagcaccgcc atttgcaaaa tatcgttgcg ctttttctcg
    47521 ccgccggaaa aaccaacgtt taccgaacgg gttaataaat cttccggcat cttcaggaga
    47581 gcgattttct cttccatcaa atcctgaaaa tcaaagcggt cgagcgtttc ctggccgcga
    47641 tagctgcgca ccgcattaag tgccgtttgc aggaaaaact ggttactgac gccgggaatc
    47701 tccaccggat actggaaggc cataaagatg ccttcgcctg cacgatcttc cggcgacagc
    47761 gcaagcaaat ctttgccttt gaactcaacc gtgccgcccg tcacttcata atcttctcgc
    47821 ccggcaagcg ttgccgataa ggtacttttg cccgaaccgt ttggccccat aatggcgtga
    47881 acttcgccgg gacgaacgtc gaggcttaat ccacgcagga tagctttatc ttccacgctg
    47941 acgtgtaaat ctttaatact taacatgttt attccttatc cgacgctgtg ttcaagactg
    48001 atggcgagga gtttttgtgc ttcaacggca aattccagcg gcagttctga gaacacgtct
    48061 ttgcagaaac cgttaacaat catcgagatg gcgtcttctt cgctaatccc gcgttgcagg
    48121 cagtaaaaca gttgatcttc accaatacgt gatgtcgttg cctcgtgctc cagttgtgcg
    48181 ctgttattgc gacattcgac atacgggaag gtatgcgccc cacaattagc gccaatcagc
    48241 attgagtcgc actgagtgaa attgcgcgca ttggttgccg tcggcatgat tttcactaag
    48301 ccgcgataac tgttctgact atgtccggca gagatccctt tcgagataat ggtcgatttg
    48361 gtgtttttac cgatgtggat catcttggtg ccggtatccg cttgctgatg accgctggtc
    48421 agcgccactg agtaaaactc accaatggag ttatcgccgc gcaaaatgca gctgggatat
    48481 ttccacgtaa tcgctgaccc ggtttctgat tgcgtccatg acattttgct gttttcgcct
    48541 tcgcacaaag cacgcttggt gacgaaattg agaataccgc cggtgttgtt atcgccaggg
    48601 aaccagtttt gtaccgtgga atatttcacc tcggcgtttt tatggatgat gacttccacc
    48661 accgccgcgt gtaactgata gctgtcacgc accggagcgg aacagccttc aatgtagctg
    48721 acgtagctgt cttcgtcggc caccagaatg gtgcgctcaa actgcccggt tttttccgcg
    48781 ttaatgcgaa aataggtgga aagttccatc gggcagcgca cgcctttagg cacataaata
    48841 aacgtaccgt cagaggctac cgccgcatta agcgcggcaa agaagttgtc atttcccggc
    48901 accacagtgc cgagatattt acgcaccagt tccggatgat cgtggatcgc ctcaccaaag
    48961 gaacagaaaa taattccctg ctccgccagt ttttcgcgat aagtagtggc aaccgaaaca
    49021 gagtcgaaaa tggcatccac cgccacctct ttgccttccc gcacgggaac gcccaactgc
    49081 tcaaacgccg cctccacctc tttacttaaa aaggcgttcg cgccagtttg ctgcaccgcg
    49141 ccaggttcag acgcgcaagt gtcgtcacaa ttaccgcacg atggtgctga gtagtaactg
    49201 taatcctgat aattcagctt gtcgtagtgc gctttcaacc agtgcggttc ttccatctcc
    49261 agccatgcgc gataggcgtt tagacgaaac tccagcatcc actccggctc attacgcttc
    49321 gccgaaattg cgcgcaccac ctcttcgttt atcccctttg ccagctcatc ggtggctaac
    49381 tgggtgaaga atccttcttt ataattcagc gggccgccgg tccaggtttt gacatcgtca
    49441 gttgcttcag tattacgaga catagtaccg cctatacccc aaagctttcg ccacagccac
    49501 attcattctg ggctttaggg ttgtgaaatt tgaatatctg attaagtcct tcacgaacga
    49561 aatcgacttc cgtgccatca ataaacggca tcgcttgcag cgggacaaac agcttcgcgc
    49621 cgtcgtgttc aaacagcaga tcgtctttgt ccggctcgct aacactgtcg agcacatagc
    49681 caaagcccgc gcagcccgtt tgcttcacgc ctaagcgcac gccgaccata cccggctgct
    49741 ttgccaccag ctcacggatg tgtatcgccg ctgcgggtgt cagcgttaag ccttgccagg
    49801 cgaaatcttg tgggttaaag gttcctgaat gcatgtccat cgatttacct cacttcatcg
    49861 ctttcagcgt ataacagcat gctagtgata atgattatca gttcaaccca gcaaacgcag
    49921 gggctttacc gtaaaacatg ctttttgcct gcttttaata agcatagacc ctgatgtgtg
    49981 ggttaacagg cacgctaaga attaggtatc tcattgttag ataatggtta ttatctaagg
    50041 agcattaagg ctgtgatgaa aaagaaaaag ttgtattgaa aatgactatt taagagatag
    50101 gtaaaaaagt gcagcgttca gaaataagaa aacccttaag tctgtgcgac acaggcttaa
    50161 gggtttctac cccatccggc gcttatctcc ggcactctca gtggcttagc tcttgaaggg
    50221 gcgataagaa taatctcata aagctaaccc gccgttttaa cacaaactgc gattagtatt
    50281 atttttgaac aatatcaggc ggtagataag cagtattaag aaggtcatcg aacctggacg
    50341 gaggttaatc caggtcgatt tggcgaactt gcggcattaa gtcaggatca atgcacgccc
    50401 agacgccagg caaagtagat ttcttctttt aattcagtag aagagagagt aagcaggtca
    50461 gcaaattcaa gttctagttg tttcagacgt ttgagatatt gggcaggtga aagattgctc
    50521 tggtcacggc gtaaaaattc aatggccagc tgggtgggat caagttgagt agacatagca
    50581 tcctcgcttt tagacaagac gtgcacagta taccaccgtt tactgtgcag ataatgacca
    50641 aaagcaatat gcgtcacact tttctggtga caacgtcaca aaatggcggt cgtcaatcgt
    50701 gacgaacagc acaaacgccc tttctcatcg aagatttcaa tctgccagac ctggtgacgc
    50761 gaaccgagat gcaacggttt acatatgccg cgcacccgcc cttctcgtgc cgagcggacg
    50821 tgattagcat tgatttccag accaaccact ttttgctcac cttcggtaca taaataaccg
    50881 gcaacggaac cgatactttc ggccagtacc acggatgctc ctccatgcag caacccgaaa
    50941 ggctgctttg tccgcgagtc tactggcatt gtcgcttcaa gggtgtcatc accaatatgt
    51001 tcaaagcgaa tatccaggaa ccccaccatg tttccttcac ccatagcatt cagtgcttcc
    51061 agggtgattt tccgtttcca tatcatttaa taatctccag taaagcctgc acaggatggc
    51121 gtacccccgt gccttcaacc cgttttacct ggctacggca ggaatatccg gtcgccagac
    51181 agcggttacg cggcagtcgc tgcatagcct gatgccagga taactcatag atcccgagcg
    51241 aattttcatg gtttttcgct tcatgtccgt aagtccctgc catgccgcag caacccacgc
    51301 tgacattttc cagtttcgcg ccaaaacggg caaatatcgc ggcccattgt gctggcgcgc
    51361 ccggcaaagc ggtaacctcg gtacagtgac caaagaaata ccatgattca ccgctgactg
    51421 tagccactgg atgtgactca agtgcgcttg ccagccattc attcgcaagt aagacgttaa
    51481 actcgccacg ttcctcgccc agcgccagtt tatattcatc gcgataacaa agtaccagcg
    51541 ccggatcgac gcccaccatt ggcatcccaa gcttagccat acggttgagg aaatccgccg
    51601 tctttttcgc cgtcttcgca aaacgattaa gaaagccttt gatatgctgg gctttgccat
    51661 ttggcgaaaa tggcagtaac acaggctgga aacctaattt ttcaaccaga cggacaaaat
    51721 ccgccaccac ttgcgcatcg taatagctgg taaaggggtc ctgcaccacc aacaccgtgc
    51781 gcgctttctg ctctgcattg agagcttcaa gctgttccag cgtcatgttt gccgagcgat
    51841 gccccaccat ttgttgttgt agcgagggga ccgacagcag tggcaaatca accatgccga
    51901 tatgtttttc cgagagtttg cgcaccagcg gctggttaat gaagaagtta aaggttttcg
    51961 gcgcgcgtgc catcagcggt gcgtagctct cgaccgtagc gacgaggtgg tcgcgcagcg
    52021 ggcgtaaata acgggtgtga tagagctgca ggaaacgaga gcgaaactcc ggcacatcaa
    52081 ttttgatggg gcactgggtc gaacacgctt tacaggccag acagcccgac atcgcctctt
    52141 tgacttcgtg tgagaagtca tattcgcctt tattcgcatg ccagctattg cgcgtgcggg
    52201 caattaacgt ccgtaaactg acgccggatt caggcagttc ttgttccagt ttgagtggat
    52261 caacgccgcg atccgccaac aaacgcagcc attcacgcac cagcgttgcg cgccctttcg
    52321 gtgaatgaat ccggttctgg gtgatcttca tcgacggaca catcggacta cgggcatcaa
    52381 agttgaagca taaaccgttg ccgttacact ccatcgcacc gcgccactgc tggcgtaccg
    52441 caatggggat ctgccgatcg aatgtaccgc gcttcaccgc gtccactttc atcatcggcg
    52501 catcgagacc ttctggcggg caaatcttcc ctgggttgag tcggttatgc gggtcaaatg
    52561 ccgctttcac tttgcgcagt tctgcaaaaa gttcctcacc gaaaaacgcc gggctgtatt
    52621 cagcgcgaaa acctttgccg tgctcgcccc acaacaaacc accgtatttc gcagtcagcg
    52681 ccaccacgtc atcagagatt tgcttcatca aaatctcttg ttgcggatcg cacatatcca
    52741 gtgccggacg gacgtgcaaa acacctgcgt cgacgtgacc gaacataccg tagcttaagc
    52801 cgtggctgtc gagcagcgcg cgaaattcag caatataatc ggccagatgt tccggcggta
    52861 cgcaggtatc ctcagcaaac ggaattggct tagcggcacc tttggcattg ccaagcagac
    52921 caacggcttt tttgcgcatt gcatagatac gttcaacccc cgccagctcg cggcacacct
    52981 gccagccaat cacacctgcc tgttggctgg caatcagctc atcaagccgc acacagagtg
    53041 cattaactcg ctcgtcaatc agcgcctcat catcaccagc aaattccaca atgttcagcc
    53101 cgagcatctc tttgtcaggc acatcggtaa tcaactcgct gacggaatgc cagacaatat
    53161 cttcccgcgc cagattcagc acttttgagt ccaccgtctc taccgaaagc gcccgcgcct
    53221 caaccataaa cggcgcgtta cgcagcgcgg agtcaaaaga gtcatatttg acgttcacca
    53281 gacggcgcac tttaggcaag ggcgtaatat ccagccgcgc ttcggtaata aaggccagcg
    53341 tcccttctga acccgtcaga atgcgcgtca ggtcgaactc ggtcatctca tcgttaaaga
    53401 catgacgcag atcgtaaccg gtaagaaagc ggttaagttt ggggaagttg tcgatgatta
    53461 actggcgttg cagacggcaa cgttgataaa ccgtgttata aattcgcccg attgtggtat
    53521 tggttttacc cagcgtttcc gccagttcga cgggtaaagg ttgcgtatcg agaatatcgc
    53581 cccccaacaa caccgcgcgt acgccaagta cgtgatctga cgttttgcca tagaccagcg
    53641 atccctgacc ggatgcatcg gtattgatca tcccgccgag cgttgcccgg ttgctggtcg
    53701 aaagttctgg tgcaaaaaag tagccgaacg gtttcaggta ctgattgagt tgatctttta
    53761 tcaccccggc ctcaacgcgc acccagccct cttcagggtt aatttcgatg atgcggttca
    53821 tatggcggga catatcaaca ataatcccct ggttgagcgc ctgaccgtta gtgccggtgc
    53881 cgccgccgcg gggggtaaag atcagcgatg aatagcgttc ctgcgcggca agacgggcga
    53941 tcagcgccac atctgcggtt gaacgcggaa ataccaccgc atcggggaga agttggtaaa
    54001 tactgttgtc ggtcgacatt gtcagacgat cggcataact tgtcgccgta tcgccggtaa
    54061 aaccttgttg ctccagctct tgcaaaaaat taagcaccag ttgaacgacg ccgggtgcct
    54121 gggaaatctg tggaatcatt atattgaccc tttcctgcgg tctgtgatgt aggtcgatac
    54181 actattcttt caggcggctg caatagcgca ctgaaaggtg atgtttattt actctatgga
    54241 tttcgagttg caggaaggcg gcaagcgagt gaactccagg agcttacaat agtaagtgac
    54301 tggggtgaac gaacgtagcc gcagcgcatg caacttgaaa tacgacgagt aaatcgtttg
    54361 cgtgttgcct gagttgttgt accacatttt tttctaacac gcccatcaga attaagggca
    54421 gaatcggcct gttaaaaacc gctgaaattg ctcatcatta tgcaggtgag tttcgcgtgt
    54481 tcacgtcgcg tcgacgattt gacgcacaaa aaaggtgaaa agtagttatg gtaaatgttc
    54541 gtcagcccag ggatgtcgca caaattctgc tttcggtgct gtttttagcc atcatgattg
    54601 tggcatgtct gtggattgtt caacccttta ttctcggctt tgcatgggcc ggtacggtgg
    54661 ttatcgccac ctggccggta ttgttacgtt tgcaaaagat catgtttggc cgccgctctc
    54721 tcgccgttct ggtgatgacg ctgttattag tgatggtgtt tatcatccct attgctttgc
    54781 tggttaacag tatcgtcgac ggcagcggcc cgctaattaa agccatttcc agcggcgaca
    54841 tgacgttacc cgatctggcg tggcttaata ccattccggt gattggcgcg aagctgtatg
    54901 caggctggca caacttgctg gatatggggg gtacggcgat catggcgaaa gtccgccctt
    54961 atattggcac caccaccacc tggttcgttg ggcaggcggc gcatatcggg cgctttatgg
    55021 tgcattgtgc gctgatgctt ctcttcagtg ccctgctgta ctggcgcggt gaacaggtgg
    55081 cacaaggcat tcgccatttt gcaacccgtc tggcaggcgt tcgcggagat gccgccgtgc
    55141 tgctggcggc acaggctatc cgcgcggtgg cgctgggtgt ggtggtgacg gcgttagtac
    55201 aggcagtgct tggcagtatc ggcctcgccg tatccggcgt accttatgca acgttgctaa
    55261 cggtgttaat gatcctctcc tgccttgtcc agcttggccc gttgccggta ctgattccgg
    55321 cgattatctg gctctactgg accggcgata ccacctgggg aacggtattg ttagtgtgga
    55381 gcggtgtggt tggcacgctg gataacgtca tccgcccaat gttaattcgc atgggtgccg
    55441 atttaccgct gatcctgatc ctctctggcg ttattggtgg tttgattgct ttcgggatga
    55501 tcggtctgtt tattggtccg gttctgttag ccgtttcctg gcgtctgttt gccgcgtggg
    55561 tggaagaagt cccgccgccg acggaccaac cggaagaaat tctcgaagaa cttggcgaaa
    55621 tcgagaaacc gaataagtaa tttctcatca ggcggctctg ccgcctgatt gttaatcacc
    55681 gctaattgat atttgcttgc tcttccccat attttcctgc ttaccgccat tcagct
//
LOCUS       DAJTYZ010000012        49807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000012 DAJTYZ010000000
VERSION     DAJTYZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.012"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..445
                     /locus_tag="OO596_000712"
     CDS             <1..445
                     /locus_tag="OO596_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6716657.1"
                     /translation="LVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAYDGT
                     VFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRESLAR
                     KIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(494..1993)
                     /gene="pitB"
                     /locus_tag="OO596_000713"
     CDS             complement(494..1993)
                     /gene="pitB"
                     /locus_tag="OO596_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6716658.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2285..4144)
                     /gene="gss"
                     /locus_tag="OO596_000714"
     CDS             complement(2285..4144)
                     /gene="gss"
                     /locus_tag="OO596_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6716659.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4349..5215
                     /gene="yghU"
                     /locus_tag="OO596_000715"
     CDS             4349..5215
                     /gene="yghU"
                     /locus_tag="OO596_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6716660.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5338..5586)
                     /gene="hybG"
                     /locus_tag="OO596_000716"
     CDS             complement(5338..5586)
                     /gene="hybG"
                     /locus_tag="OO596_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6716661.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5599..5940)
                     /gene="hypA"
                     /locus_tag="OO596_000717"
     CDS             complement(5599..5940)
                     /gene="hypA"
                     /locus_tag="OO596_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6716662.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5933..6421)
                     /gene="hybE"
                     /locus_tag="OO596_000718"
     CDS             complement(5933..6421)
                     /gene="hybE"
                     /locus_tag="OO596_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6716663.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6414..6908)
                     /locus_tag="OO596_000719"
     CDS             complement(6414..6908)
                     /locus_tag="OO596_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6716664.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6908..8611)
                     /gene="hybC"
                     /locus_tag="OO596_000720"
     CDS             complement(6908..8611)
                     /gene="hybC"
                     /locus_tag="OO596_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6716665.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8608..9786)
                     /gene="hybB"
                     /locus_tag="OO596_000721"
     CDS             complement(8608..9786)
                     /gene="hybB"
                     /locus_tag="OO596_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6716666.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9776..10762)
                     /gene="hybA"
                     /locus_tag="OO596_000722"
     CDS             complement(9776..10762)
                     /gene="hybA"
                     /locus_tag="OO596_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6716667.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10765..11883)
                     /gene="hybO"
                     /locus_tag="OO596_000723"
     CDS             complement(10765..11883)
                     /gene="hybO"
                     /locus_tag="OO596_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6716668.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12072..12359)
                     /locus_tag="OO596_000724"
     CDS             complement(12072..12359)
                     /locus_tag="OO596_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6716669.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12478..13365)
                     /locus_tag="OO596_000725"
     CDS             complement(12478..13365)
                     /locus_tag="OO596_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6716670.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13522..14562
                     /gene="gpr"
                     /locus_tag="OO596_000726"
     CDS             13522..14562
                     /gene="gpr"
                     /locus_tag="OO596_000726"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6716671.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14602..15096)
                     /locus_tag="OO596_000727"
     CDS             complement(14602..15096)
                     /locus_tag="OO596_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6716672.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15287..16171
                     /gene="yghA"
                     /locus_tag="OO596_000728"
     CDS             15287..16171
                     /gene="yghA"
                     /locus_tag="OO596_000728"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6716673.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16331..16756)
                     /gene="exbD"
                     /locus_tag="OO596_000729"
     CDS             complement(16331..16756)
                     /gene="exbD"
                     /locus_tag="OO596_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6716674.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16763..17497)
                     /gene="exbB"
                     /locus_tag="OO596_000730"
     CDS             complement(16763..17497)
                     /gene="exbB"
                     /locus_tag="OO596_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6716675.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17749..18936
                     /gene="metC"
                     /locus_tag="OO596_000731"
     CDS             17749..18936
                     /gene="metC"
                     /locus_tag="OO596_000731"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6716676.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19076..19735
                     /locus_tag="OO596_000732"
     CDS             19076..19735
                     /locus_tag="OO596_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6716677.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19775..20674)
                     /gene="yqhC"
                     /locus_tag="OO596_000733"
     CDS             complement(19775..20674)
                     /gene="yqhC"
                     /locus_tag="OO596_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6716678.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20868..22031
                     /gene="yqhD"
                     /locus_tag="OO596_000734"
     CDS             20868..22031
                     /gene="yqhD"
                     /locus_tag="OO596_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6716679.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22136..22963
                     /gene="dkgA"
                     /locus_tag="OO596_000735"
     CDS             22136..22963
                     /gene="dkgA"
                     /locus_tag="OO596_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6716680.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            23163..24089
                     /locus_tag="OO596_000736"
     CDS             23163..24089
                     /locus_tag="OO596_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6716681.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            24140..24397
                     /gene="yqhH"
                     /locus_tag="OO596_000737"
     CDS             24140..24397
                     /gene="yqhH"
                     /locus_tag="OO596_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6716682.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24440..26659)
                     /locus_tag="OO596_000738"
     CDS             complement(24440..26659)
                     /locus_tag="OO596_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6716683.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(26770..28182)
                     /gene="ftsP"
                     /locus_tag="OO596_000739"
     CDS             complement(26770..28182)
                     /gene="ftsP"
                     /locus_tag="OO596_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6716684.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(28257..28994)
                     /gene="plsC"
                     /locus_tag="OO596_000740"
                     /gene_synonym="parF"
     CDS             complement(28257..28994)
                     /gene="plsC"
                     /locus_tag="OO596_000740"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6716685.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(29227..31485)
                     /gene="parC"
                     /locus_tag="OO596_000741"
     CDS             complement(29227..31485)
                     /gene="parC"
                     /locus_tag="OO596_000741"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6716686.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(32031..32513)
                     /locus_tag="OO596_000742"
     CDS             complement(32031..32513)
                     /locus_tag="OO596_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6716687.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32566..32958)
                     /gene="ygiW"
                     /locus_tag="OO596_000743"
     CDS             complement(32566..32958)
                     /gene="ygiW"
                     /locus_tag="OO596_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6716688.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33110..33769
                     /gene="qseB"
                     /locus_tag="OO596_000744"
     CDS             33110..33769
                     /gene="qseB"
                     /locus_tag="OO596_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6716689.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33766..35115
                     /gene="qseC"
                     /locus_tag="OO596_000745"
     CDS             33766..35115
                     /gene="qseC"
                     /locus_tag="OO596_000745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6716690.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35225..35806
                     /gene="mdaB"
                     /locus_tag="OO596_000746"
     CDS             35225..35806
                     /gene="mdaB"
                     /locus_tag="OO596_000746"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6716691.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35837..36151
                     /locus_tag="OO596_000747"
     CDS             35837..36151
                     /locus_tag="OO596_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6716692.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36199..38091)
                     /gene="parE"
                     /locus_tag="OO596_000748"
     CDS             complement(36199..38091)
                     /gene="parE"
                     /locus_tag="OO596_000748"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6716693.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(38120..38701)
                     /gene="yqiA"
                     /locus_tag="OO596_000749"
     CDS             complement(38120..38701)
                     /gene="yqiA"
                     /locus_tag="OO596_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6716694.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38701..39528)
                     /gene="cpdA"
                     /locus_tag="OO596_000750"
     CDS             complement(38701..39528)
                     /gene="cpdA"
                     /locus_tag="OO596_000750"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6716695.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39553..39975)
                     /locus_tag="OO596_000751"
     CDS             complement(39553..39975)
                     /locus_tag="OO596_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6716696.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39976..40605)
                     /gene="nudF"
                     /locus_tag="OO596_000752"
     CDS             complement(39976..40605)
                     /gene="nudF"
                     /locus_tag="OO596_000752"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6716697.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            40810..42291
                     /gene="tolC"
                     /locus_tag="OO596_000753"
     CDS             40810..42291
                     /gene="tolC"
                     /locus_tag="OO596_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6716698.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42439..43110
                     /locus_tag="OO596_000754"
     CDS             42439..43110
                     /locus_tag="OO596_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6716699.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            43116..44276
                     /locus_tag="OO596_000755"
     CDS             43116..44276
                     /locus_tag="OO596_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6716700.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(44314..45102)
                     /gene="ygiD"
                     /locus_tag="OO596_000756"
     CDS             complement(44314..45102)
                     /gene="ygiD"
                     /locus_tag="OO596_000756"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6716701.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45245..46018
                     /gene="zupT"
                     /locus_tag="OO596_000757"
     CDS             45245..46018
                     /gene="zupT"
                     /locus_tag="OO596_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6716702.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(46076..46246)
                     /locus_tag="OO596_000758"
     CDS             complement(46076..46246)
                     /locus_tag="OO596_000758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716703.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46507..47160)
                     /gene="ribB"
                     /locus_tag="OO596_000759"
     CDS             complement(46507..47160)
                     /gene="ribB"
                     /locus_tag="OO596_000759"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6716704.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47264..47412)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47534..47824
                     /gene="ubiK"
                     /locus_tag="OO596_000760"
     CDS             47534..47824
                     /gene="ubiK"
                     /locus_tag="OO596_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6716705.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            48107..48658
                     /locus_tag="OO596_000761"
     CDS             48107..48658
                     /locus_tag="OO596_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR6716706.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            48718..49725
                     /locus_tag="OO596_000762"
                     /pseudo
     CDS             48718..49725
                     /locus_tag="OO596_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT        12491 a        13199 c        12353 g        11764 t
ORIGIN      
        1 gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata tgcttgcctg
       61 tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa ttcctggcgc
      121 ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct catggctggc
      181 aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc tggtcatcaa
      241 actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac gggaatcgct
      301 ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac tggttgatat
      361 cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga ttacggattt
      421 tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc aggaccgggc
      481 attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca gaaagaaaaa
      541 ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt ttacgctgta
      601 acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg tgtgttgtgg
      661 agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc atttgtgccg
      721 ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg gtcatcgcta
      781 cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc atgatgatcc
      841 acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc agcaggttct
      901 ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca gagatgcaca
      961 gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa ctttccatat
     1021 tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc ggatgacagt
     1081 gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt tccatcgcaa
     1141 tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg gtgacggcat
     1201 cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag ccagcagggg
     1261 caatccccac cagtaccagc atcaccaggc cgatcccttt ttgtccgtcg ttcgcgccgt
     1321 gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag aatggcggtt
     1381 tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg tcacgctttt
     1441 tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg accaggccga
     1501 cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg tttaacgcat
     1561 ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata atcgcaccaa
     1621 tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg ttccagataa
     1681 tcgccgccag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc atattcagca
     1741 acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg cccaataaca
     1801 cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc atggcacgag
     1861 tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt gcttcgtaga
     1921 acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca aggccaacaa
     1981 ataaatttag catatattag attacgccat tttgaattta cgaacggacg cattatcagt
     2041 gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag agtgttattt
     2101 cttgtgtatc agacaataag cattcattca aaacctttag aaataatcaa aacacaggta
     2161 ttccactggt taatatattc acatatgaaa taaataaata ttagaacata taaatattga
     2221 atattttgat taatccccgt actgattatt cttcataatc agtacggggt tgctacaaca
     2281 tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg atgaccagtg
     2341 attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag gtacatacct
     2401 gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg tttttctgct
     2461 cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc aggtcgatat
     2521 tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc aattcatcat
     2581 taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac cagaggatcg
     2641 gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact agcacttccg
     2701 ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta cggattggca
     2761 ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt tcccacgccc
     2821 aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc ccggcagcat
     2881 cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc tggtgcagcg
     2941 cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc atgatatgga
     3001 caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc agcccttccg
     3061 ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg atcaagcccg
     3121 cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg ccacgctcat
     3181 ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc caggagagac
     3241 gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg tcatctttca
     3301 gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct ttaattagct
     3361 cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg atcacctgac
     3421 cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat ttaccgtcaa
     3481 actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct gcaatttccg
     3541 gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga atggtggtgt
     3601 catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc agctcacgcg
     3661 tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca atacggactt
     3721 tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat tcaccgcctt
     3781 tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta ggaaatgcct
     3841 gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag aaaatctccc
     3901 acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa cggcgagcaa
     3961 attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta cggaatacgg
     4021 cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag attgctaccc
     4081 cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc gttcctttgc
     4141 tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg tgctctgatt
     4201 acctgctcag cgacattaac cggacagagg tcagactaat catacacctc atcgcagcgg
     4261 ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct acactgcttc
     4321 aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc ccgcgaaagt
     4381 ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc cggtttctgg
     4441 tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt attcgctggg
     4501 aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc tgggcgttac
     4561 tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat tctccagcgg
     4621 ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata cgcataatcc
     4681 gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga aatttggcta
     4741 cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt tctggttaca
     4801 gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg cgccgatgaa
     4861 aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg acgtgctgga
     4921 taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg cggatatggc
     4981 gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg cagagtttct
     5041 tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg aacgtccggc
     5101 ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc agttgcatga
     5161 gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg ggtaagggtt
     5221 ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca tccggcaatc
     5281 gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag ctaatcatca
     5341 cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt tggcttcatc
     5401 ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc ccagtagatc
     5461 ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac cacatacttc
     5521 aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg gaacgccaat
     5581 acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg aatcgccggt
     5641 atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct gatgaatctc
     5701 caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga tatgtaaatc
     5761 gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga cggcgctctc
     5821 ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct taacatcgtg
     5881 ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca actcatgcat
     5941 tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt acatccggat
     6001 tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt tggccctctt
     6061 caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat tgcgaaacac
     6121 cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga ccaatttttt
     6181 cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact gcactcagca
     6241 tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa tcagaaacat
     6301 acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg gcaatttctt
     6361 caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc tcttcagtca
     6421 tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc cagaacctgc
     6481 tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg tggctccagc
     6541 gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt gaagcgcagg
     6601 gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt aaacaacgcc
     6661 ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt cgacacaatg
     6721 gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc cattcccgcc
     6781 gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc taacgcttcg
     6841 acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc taagactaaa
     6901 atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc actacgtgta
     6961 ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc agcggtttat
     7021 tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg tcatcattga
     7081 agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta ctgataatac
     7141 cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg aagccaacac
     7201 ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga tcgcctttgc
     7261 cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat tcgcagcagt
     7321 gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact tccagcgtgt
     7381 tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg gtagattcgc
     7441 gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc gttttgccgt
     7501 agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca tcataagccg
     7561 gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag gagtgcttcg
     7621 cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc ggacgatacg
     7681 aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg tttttactgt
     7741 cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc gtcagccatt
     7801 ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca acaaagtcgc
     7861 tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc acgcccaaac
     7921 cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg tgcggcgttt
     7981 taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc gcctgcaggt
     8041 agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg tgaccccagt
     8101 agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc ttgttctgaa
     8161 ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc agcatttcgg
     8221 aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc agcgccgaaa
     8281 gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg atgttacgga
     8341 tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca cgaacggaag
     8401 acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc catgcatcgc
     8461 gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg gaagcccatg
     8521 ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc tcaatacggg
     8581 ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac gattatgatc
     8641 gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt aagcgcaaat
     8701 ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga agtaggcgta
     8761 accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg cacaacctaa
     8821 cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct tcgccacacg
     8881 cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca cggagtagaa
     8941 gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa agcgcagcac
     9001 gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca gactcttttc
     9061 atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga tgacaatcga
     9121 gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat agctctgcca
     9181 caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag actggtgcat
     9241 ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca gacgcttgag
     9301 agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg ccatcacgcc
     9361 gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt tgaagtgacc
     9421 cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga tcgacaagcc
     9481 acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg gatggtattg
     9541 cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg caaagccggt
     9601 gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt tcaggtcaga
     9661 gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga tgacgattaa
     9721 cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt gtggatcatg
     9781 actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga accagcacgg
     9841 ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg tgttgaatat
     9901 tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga ttttcataag
     9961 gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg tacagatgcg
    10021 gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc tgacgcggat
    10081 agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc atcagctctt
    10141 cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca ggcagaccgc
    10201 ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc agcgcaccaa
    10261 acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg cagtaacggc
    10321 aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct tttttcagtg
    10381 cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac tgtttcttaa
    10441 tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg gtccacacct
    10501 gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc tgttcccctt
    10561 gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc tggcagccta
    10621 cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg cggttttcag
    10681 cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag gaggctgctt
    10741 taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat ctttcttttg
    10801 ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc caaccacacc
    10861 gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt taacatccgg
    10921 tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc ctttatggaa
    10981 gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg gccacacgcc
    11041 gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc ctttacagcc
    11101 gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt tggcaaaacg
    11161 accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc cataggcgaa
    11221 ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga tgtgcgcaac
    11281 ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa cggttttgcc
    11341 tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg cggcaacacc
    11401 gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg ccgctttgcg
    11461 gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat cttttaatgg
    11521 gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg cgttatgttt
    11581 gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact ccagagagat
    11641 agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag attccgtaca
    11701 accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt tagtaaccga
    11761 ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta atgctgcaca
    11821 aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt tatctccagt
    11881 catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc gcaaaatcaa
    11941 taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat catgatactg
    12001 cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag caagataata
    12061 cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa cgtagaacag
    12121 gaattattct cacgaaagaa atccattacc atctctttat ccagtccata gcggcgcgtc
    12181 agaatacccg cttcccaggc actaagctgg cgatctccgg tcttttcgta catccggtgt
    12241 gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac attaaccgca
    12301 ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg gttattcatc
    12361 gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa gaacgacttt
    12421 tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat aaacctatca
    12481 ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg cggcagattt
    12541 gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat agatatacgc
    12601 ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc cctcgttgat
    12661 tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg ccacatcggc
    12721 agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat acgcgacagc
    12781 cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt tgcctgccgc
    12841 ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca gtttggttgg
    12901 atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac ctccaacgga
    12961 acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc gtgccacatc
    13021 ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg gcgttttgcc
    13081 gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat ttggcgaagg
    13141 atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag tcgccagcgc
    13201 ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca ccgcatactt
    13261 cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat agtcgtagta
    13321 atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg cactcctcag
    13381 ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc gtcactctgt
    13441 gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg tttcgaaatt
    13501 gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta cgggcagatg
    13561 caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct cggtttatgg
    13621 cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg taaagcgttt
    13681 gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc aggaagcgca
    13741 gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga tgaactgatt
    13801 atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg cggttcacgt
    13861 aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tagggcttga gtatgtcgat
    13921 atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc ctctgcgctg
    13981 gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta ctcgccagag
    14041 cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt aattcatcaa
    14101 ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga taccctgcaa
    14161 aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct gaccggaaaa
    14221 tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa agttcgtggt
    14281 ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt gaatgaaatg
    14341 gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct gaaagatgat
    14401 cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga gaacgtgcag
    14461 gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca gcatatcgcc
    14521 gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa taacgggcgc
    14581 gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa gatagcccat
    14641 cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca gtttgttatc
    14701 cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa ttgccacaat
    14761 cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc agttcagctt
    14821 ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg aaaacatcac
    14881 catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca cgaggatcaa
    14941 atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct ggaagaactt
    15001 cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa gcagccagcg
    15061 agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg tagttaaaac
    15121 gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac atatacgtta
    15181 aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat caacgcatcg
    15241 agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt ctcatttaaa
    15301 agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac cgacgccagg
    15361 catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg ttggtagcgg
    15421 tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag gtcgcgctgc
    15481 cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc ccgtggaaga
    15541 agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag ccgttctgct
    15601 gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag cgcacaaggc
    15661 gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca ttccggatat
    15721 tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt tcgcgctgtt
    15781 ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca tcaccacttc
    15841 gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta cgaaggcggc
    15901 gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta ttcgggtgaa
    15961 tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac aaacgcagga
    16021 taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac cggcggaact
    16081 ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg cagaagtgca
    16141 cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa aagcaaccgg
    16201 gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg gcctacaaac
    16261 gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta gcgcatcagg
    16321 caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc ttcaggtaac
    16381 ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg gttttgtccg
    16441 ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac gccgtaatca
    16501 ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc ttcaccgaca
    16561 gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc aagttcacct
    16621 tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga accaacatca
    16681 cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg aggttttcgt
    16741 taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca cgaaccggat
    16801 gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat acctgcgctg
    16861 caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg ttatagataa
    16921 cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct tctgcgatac
    16981 ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata aagctgttca
    17041 tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg atggttgcca
    17101 gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc tccagacgga
    17161 agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt tccagctcat
    17221 tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca gcggcgatat
    17281 cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc ttaaggcgac
    17341 gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg actacggagg
    17401 ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga tacatacccc
    17461 agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag gacgcaagtc
    17521 acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt ctcattacta
    17581 tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa aaaagtcacc
    17641 agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc gcccgcgaat
    17701 attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat ggcggacaaa
    17761 aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct cggcgcggta
    17821 aatagcgtga ttcagcgcgc ttcttcgctg gtctttgact gtgtggaagc caaaaagcac
    17881 gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac gttaacccat
    17941 ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt gctatttccc
    18001 tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg cgatcatgtg
    18061 ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat cctcagcaaa
    18121 ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt taagcatctg
    18181 cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat ggaagtccac
    18241 gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat cattatgatc
    18301 gacaacacct gggcagccgg tgtgctgttt aaggcgctgg attttggcat cgatgtttct
    18361 attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg cactgccgtg
    18421 tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg ccagatggtc
    18481 gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt gcgtttgcgt
    18541 caacatcatg aaagcagtct gaaagtggct gaatggctgg cagaacatcc gcaagtagcg
    18601 cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa acgagacttt
    18661 acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa tgaagagctg
    18721 gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg cgggtatgaa
    18781 tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca aggcgagatc
    18841 gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga cgatctgatt
    18901 gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg gacaattttg
    18961 cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc gtcccggacg
    19021 aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa agtccatggc
    19081 tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc tggcggatcc
    19141 tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag aaaacggcct
    19201 gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg cattgattgc
    19261 ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag caagtctggg
    19321 ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg tgaaaggctg
    19381 gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg accgccacgg
    19441 tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc tgccaaccat
    19501 ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt taagtggatt
    19561 gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc cgttcgttaa
    19621 acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct tgttaaccgc
    19681 tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg cctgacgatt
    19741 ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc gcccgcattc
    19801 ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg ctaaattgcg
    19861 atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg tcatggatga
    19921 tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc gaggtgctgg
    19981 tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct gccgccagtt
    20041 gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg cggctaatca
    20101 gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa ggtccggtca
    20161 gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca tccagtggtc
    20221 gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat aacgtggcgg
    20281 agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca atgtccatca
    20341 acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc ggcacctctg
    20401 acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg tattcattgg
    20461 catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa aagagaatta
    20521 tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca tacaacaaac
    20581 gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta ttaactttat
    20641 ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta ggaataatgc
    20701 ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga aataggcaag
    20761 acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga aaaacatcag
    20821 gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg aacaacttta
    20881 atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt ttacgcgaac
    20941 aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg aaaaaaatcg
    21001 gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa tttggcggta
    21061 ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt cgcgaacaga
    21121 aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc aaatttatcg
    21181 ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa acgggcggta
    21241 aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca accggttcag
    21301 aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag gcgttccatt
    21361 ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac accctgccgc
    21421 cgcgtcaggt ggctaacgac gtagtggacg cctttgtaca caccgtggaa cagtatgtta
    21481 ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg ctgacgctaa
    21541 tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc gccaacgtca
    21601 tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg caggactggg
    21661 caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac gcgcaaacac
    21721 tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc gctaagctgc
    21781 tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag cgtattgacg
    21841 ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc cacctctccg
    21901 actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa gagcacggca
    21961 tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt atatacgaag
    22021 ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt ccagacttaa
    22081 gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg aacgtatggc
    22141 taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg gactgggcgt
    22201 ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag aagtgggtta
    22261 tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca aagccctgaa
    22321 aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga acgacgacca
    22381 caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg attatatcga
    22441 cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag catggaaagg
    22501 catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca acttccagat
    22561 ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa accagatcga
    22621 acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac acaaaatcca
    22681 gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc agaaagtcat
    22741 tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc gctggcatct
    22801 ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg ccgaaaactt
    22861 tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa aactcgatca
    22921 gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa attctcccgg
    22981 tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc gcgtcccgcc
    23041 cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat gcagagccgg
    23101 aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga ggtgaaaaac
    23161 agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta cacgcgcagc
    23221 ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat aaatcagatg
    23281 ccagtacacc ttatttcggt gaaaccggag agcgggcgat aacttcagcg cgtattcaac
    23341 aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg ctggattatg
    23401 atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt gcgataaccc
    23461 aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa gatgataaag
    23521 aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc attgacgata
    23581 ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa aaaacgctgg
    23641 ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa ctctttgaac
    23701 atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac cgccaggcgg
    23761 ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa gatatgcaaa
    23821 tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg ctattttcac
    23881 gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt catgttcgtt
    23941 ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg cgcgaaggca
    24001 agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag cagattgagg
    24061 caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag ccggaacaat
    24121 aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac
    24181 ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga gcaatgtaca
    24241 aacattaaat gccaaaatcg cccggcttga gcaggatata aaagcactac gcccacaaat
    24301 ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgctc aggactattt
    24361 tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc ggcagcatgt
    24421 cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc ttcgcaccag
    24481 cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc gtctgacgct
    24541 gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc tggcgacgag
    24601 cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg ctgccaatca
    24661 gatgcttttt gcccatcgct tccagcgccg ggcggattaa cggccagttt gccggatcgt
    24721 ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt acgaagacgt
    24781 cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac atggtggttg
    24841 agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa cgatgctttt
    24901 tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc gcagagatga
    24961 aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg aacagctctt
    25021 taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc ccttcttcgg
    25081 tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac tctttgatat
    25141 agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgatgagg atttttttaa
    25201 tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg ttagtgtcca
    25261 tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt tcagcgcgtg
    25321 gcgatttgca gtgcaacata tacatgttgg cagttggccc accgagatcg gaaatcacgc
    25381 ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc gaatcttccg
    25441 aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa cagccgccaa
    25501 agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga atacgggcat
    25561 tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg tccatctctt
    25621 cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg tgtttttgca
    25681 tcaatgcgcg gacgcagcct gggttggttt cgtggtgcag aatacgcgaa gcatgggcgt
    25741 acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag gttttttccc
    25801 acggtttcgg gcgcggtggc tgcacggtta cggctttggc ttcctgcttt ttcggtgcca
    25861 ccggtttgtt atccgcgcac ggcaaatctt caccatacgg atgcgggatt gggtcgattt
    25921 ttccaggggt atcaagacgg ggggaatcca cgccgctcca gccaggcagc gcctctttca
    25981 cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc atcgccagac
    26041 ggtgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg tcggctttcg
    26101 aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgt gcggtacggc
    26161 gcagactagc ctcaataccg ccgaggatca ccggtacatc tttccacgcc tctttacaac
    26221 gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta tccggcgtgt
    26281 aggcatcgtc atgacgtaaa cggcgatcgg cggtataacg gttgatcatc gaatccatgt
    26341 tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag tcgtctttgc
    26401 tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc agcatacgac
    26461 cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta accaaaatga
    26521 tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc aaaaacggtg
    26581 ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga tccggttgga
    26641 tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca aaggccgggg
    26701 attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct cctcgggcga
    26761 gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac ggtccgccat
    26821 ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct gaccgaaata
    26881 aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc tgtcttccgg
    26941 gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa tatggaacgc
    27001 ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg cggtgacatc
    27061 aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca gactgatatc
    27121 gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaagcgca tcggaagact
    27181 gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg tagaaaccag
    27241 aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg cttcgccaca
    27301 ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc gctcgcccgg
    27361 tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat cgccagaaat
    27421 cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt tcgacgcgtt
    27481 cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt gtacaccgtt
    27541 aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt ctggcgtacc
    27601 aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacat cataatggtt
    27661 ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg caaggccgtt
    27721 atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag tagctgcgtt
    27781 ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc gtgccggacc
    27841 gcccatcagc gggccaggta cctgtagccc ggccaccgtc attgagacat tttctgtcag
    27901 gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag tcggccccag
    27961 gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg accagtgcgc
    28021 acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg gcggaacggg
    28081 tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg cacaaagtgc
    28141 aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc catgtaaaac
    28201 tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt gccgacttaa
    28261 acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat tttttgttcc
    28321 attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata ctgactgacg
    28381 tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt aagattaatc
    28441 ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc aattgccgcg
    28501 tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt tccttccggg
    28561 aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc aatggtgccg
    28621 tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca gtacaactgc
    28681 ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg cggttgcaca
    28741 atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata gatagcattg
    28801 ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa cagcggcgca
    28861 agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg gctgaaaagg
    28921 cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat aataagacga
    28981 aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac gtcacctgaa
    29041 atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta atcgcaacgc
    29101 atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa tacgctgccg
    29161 gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca ggccctcccc
    29221 ggcatattac tcttcgctat caccgttgct ggcacggcga ggagagtcga tctcaacacg
    29281 atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac cagtgacttt
    29341 ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct
    29401 ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg cttctgccga
    29461 tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cgcttaccgg
    29521 gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat
    29581 caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct tacctgcacg
    29641 gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat
    29701 cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc caggcggcaa
    29761 cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg taatcgggtc
    29821 gatggcatag ctacgaccgg tggaatcaac aaacactacc gattggttgc tcttaccttt
    29881 caccgccact ttgaagctat cacccgcttt ataattcagg cccggcgcgt cgatatcatg
    29941 gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag gttcagacgg
    30001 cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg
    30061 atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt
    30121 acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct cactctgctc
    30181 accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc
    30241 ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc
    30301 gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca aaccttccag
    30361 gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc
    30421 gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc
    30481 atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg tggtagcgaa
    30541 gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccagacg
    30601 ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt
    30661 gcgcatttgc gcagcaattt gctccagtac acgcgcacct gaaacctgat gcggtaatgc
    30721 gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgta ctgaaccacg
    30781 tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg cttcagtcgg
    30841 ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg gctggtcgat
    30901 taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat cggtcgccat
    30961 gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacgag caggcagcat
    31021 tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag
    31081 ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc
    31141 gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg
    31201 gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat cgccgtgcgg
    31261 atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc
    31321 attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc
    31381 accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt
    31441 ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc
    31501 aactgattcg ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg
    31561 tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg gcgataaagg
    31621 catcaatgtg ccttgatata caacgctttg ctgtagactt gatcggcata gagggcagca
    31681 caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg attcgagaag
    31741 ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgacta taagtatatg
    31801 gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca taagtgcggc
    31861 ttgctcatgg gtacccccga gacggcttac ggcgcaccgg ataataaatt tcgcgtatac
    31921 atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg gcattgttcg
    31981 ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca tcacgctaac
    32041 ggcacataaa catccgtttc cagtcgctgc tccgtcaccc cttcggcaag attggtgtag
    32101 tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca gtggcgaata
    32161 atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc cacggcataa
    32221 cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat cggttcgcta
    32281 acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg gtcgtcccag
    32341 gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt acgccacatg
    32401 ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg caacatcgcc
    32461 acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt cattttgcct
    32521 ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg gatttacttt
    32581 gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt caccctgaat
    32641 ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtggt cgatatcaac
    32701 attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc gttcaacgat
    32761 attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt ctacagtcgt
    32821 tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg atgggccaga
    32881 aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta ctgcgattac
    32941 tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag catttaacaa
    33001 gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta cagagcgcgt
    33061 tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt
    33121 actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta gtaaaatggg
    33181 ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc
    33241 ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg atattttgcg
    33301 cgaatggcga gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc gtgatgcgtt
    33361 agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta aaccttttgc
    33421 gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg gccaggccag
    33481 caacgagctg cgccacggta acgttatgct cgaccccggc aaacgtatcg ccacgctggc
    33541 tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac tgatgcgtaa
    33601 cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct gggacgaaga
    33661 ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga
    33721 ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa atttacccaa
    33781 cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc ggtgacctgg
    33841 ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga attgttcgac
    33901 acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga aatcaacgcg
    33961 gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga tgacgatgcg
    34021 ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg cgataacgga
    34081 gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa
    34141 gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg catcgttgtt
    34201 ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg gcaattgatc
    34261 ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact gggtcgtgaa
    34321 ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa
    34381 ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc gctaaatcaa
    34441 ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc cgatgcagct
    34501 cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc gcagctctct
    34561 gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg gatcgatcgc
    34621 gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact ggataacctt
    34681 caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat ggatatttac
    34741 cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca cagcatcaaa
    34801 cgcactggac aaccgctatt gctaagtttg ttggtgcgaa accttctgga taacgcagtg
    34861 cgctacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa tttcatcgtg
    34921 agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat
    34981 cgcccccccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc
    35041 gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg atttgaggcg
    35101 aaggtgagct ggtaagggta agacttttgc taaattcgca aaagactttg cacattttgc
    35161 taattttacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac cttgaggtta
    35221 aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca
    35281 actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt
    35341 tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa actttctctg
    35401 ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt ggacagtgaa
    35461 aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca gcgatggccg
    35521 tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg gcaaaaaata
    35581 tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag atcaattctt
    35641 ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc aattccttgg
    35701 catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg
    35761 ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag agctcaggct
    35821 ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc ctggtcaaca
    35881 tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga
    35941 aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat
    36001 ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc ttgaagcgca
    36061 tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg tgctggagat
    36121 gaatattcgt attctgcagc cagggattta atcctgcctt gtttgcccgg ccatcctgac
    36181 cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct cttgcaacca
    36241 gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg cgtcagtacg
    36301 ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg gatcaagcgt
    36361 ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct ggacgttcgg
    36421 cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt ccgtcagcgc
    36481 gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga cgtaaacgtg
    36541 accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca gcgtggcaat
    36601 gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac gaagctggct
    36661 cagatcgtcg ctgttaggat cgataccgat cgctaccgaa atatcatgca cttcctgcga
    36721 ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt tcagcggcat
    36781 gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc
    36841 cacgaggaac agttcggtac ggttaaggtc ctgcgcggta caatcagcca gtttgccagg
    36901 cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac gcatacgacg
    36961 ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt tctggttcag
    37021 ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt gacgcgaaga
    37081 gagacgctct ttcgtctgcc cggcaaactg cggatcctgc attttcaccg acagaacata
    37141 ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca gaatgttgcg
    37201 atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat taacatgggt
    37261 accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt caccgccttc
    37321 cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat taccgataaa
    37381 cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat cattcagacc
    37441 gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg taatctcaac
    37501 gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa cagaaaatcg
    37561 cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg tattgcgttt
    37621 accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa aggcgatgtt
    37681 ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg cgttaaccac
    37741 cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag agaatttacc
    37801 gcctgcatgc agacggcaaa gaatcaattc aaccgccggg acaccctctt ccgggtgaat
    37861 atccaccggc atcccgcgcc catcgtcaat aacttctaac gactggtcag catgtaagat
    37921 tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat caataacttc
    37981 ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc ggcgaaccgg
    38041 ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca tggtttaagt
    38101 tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg atcgggttga
    38161 aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc tggcggcagg
    38221 aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc tgttgcagca
    38281 gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga tcgtaaatat
    38341 ggcgtgactc tagcacatat tgctgcccgg tgtatgggtt ctcgttctga ccgagatagt
    38401 ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc agcataaaac
    38461 attgtgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata cccagcgaat
    38521 cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa ggatacggcg
    38581 gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag ttttttaaca
    38641 agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca
    38701 tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc gatgcacctc
    38761 ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg cgatagtatc
    38821 cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag
    38881 gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca gcaagtattt
    38941 gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca gactgtgttg
    39001 atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca acgtatggcg
    39061 ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc tcagctcacc
    39121 gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct caccaataaa
    39181 cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa
    39241 atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa
    39301 atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga caatcaggtc
    39361 gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt
    39421 tacccctaac agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa tttgtaaaat
    39481 cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat ggtgtcctta
    39541 ggtttcacga tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa
    39601 ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca actttttatt
    39661 aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca cttcagccac
    39721 catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg
    39781 cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa tcgtcagccg
    39841 atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa
    39901 caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag gaaagtcagg
    39961 tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca gctgcagcca
    40021 ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc actggtatgc
    40081 ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca gaccatgaat
    40141 accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct cactggtgcc
    40201 ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca gtcccgcctc
    40261 ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcta tcatcccggc
    40321 aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc gaatctgctc
    40381 aatcagcaca acttcatcgc gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg
    40441 ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac gatgacgaaa
    40501 tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac
    40561 atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta atgtcctggc
    40621 actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga gcagtcatgt
    40681 gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc
    40741 ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa
    40801 ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc tgggttcagt
    40861 tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg
    40921 gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc
    40981 agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc
    41041 gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac tcaatccatt
    41101 tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg gattcaggac
    41161 gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac
    41221 gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc gatctaccgt
    41281 caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac cgacgtgcag
    41341 aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt
    41401 gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga actggcggcg
    41461 ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa
    41521 gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct ggcgcgcgag
    41581 caaattcgcc aggcgcagga tggtcactta ccgacgctgg atttaacggc ttctaccggg
    41641 atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac
    41701 gacagcaata tgggccagaa caaagtgggc ctgagcttct cgctgccgat ttatcagggc
    41761 ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc cagcgagcaa
    41821 ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa caacattaat
    41881 gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca aagctcatta
    41941 gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt gttggatgca
    42001 accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa ctacctgatt
    42061 aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct gctggcactg
    42121 aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc gcaaacgccg
    42181 gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt cgttcagcaa
    42241 acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg acgacgacga
    42301 cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt
    42361 cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac
    42421 tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa
    42481 aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg
    42541 gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca
    42601 gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa
    42661 gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa
    42721 ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca ggcgcaggcc
    42781 cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat ggggcgtctg
    42841 atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa cccagccagc
    42901 ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc
    42961 cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac tactaccgtt
    43021 acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagcgcaacc
    43081 ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc
    43141 gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg
    43201 agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt gaaaagctgg
    43261 aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca
    43321 gcgatgagct gatgaccaaa ttccgcattc caaaacgcac ctggagtttt gtgcgtcagt
    43381 catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcatgg gatggcactg
    43441 gcgaacctaa actgctggaa aataacgccg atacaccaac gtcactatac gaggcggcgt
    43501 tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg
    43561 accagtttaa cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg cgtgaacagt
    43621 atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca
    43681 ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc ctctacattg
    43741 acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag gtaatttcca
    43801 acctgttcaa gctgtatcca tgggaattta tgttgcgtga gatgttctcc accaagctgg
    43861 aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaagcgc
    43921 ttctaccgct actatgggag atgttcccga atcacccgaa cctgctgccc gcttattttg
    43981 cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc tcccgtgaag
    44041 gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg
    44101 gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata
    44161 tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt cgtgaagacc
    44221 gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttatt gagtaatcaa
    44281 tgatgccgga tggcattcgc catccggtaa ttgttaacct atctgcaccg acagcatact
    44341 caggctgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc catcccacgc
    44401 acctaagaca tacaacaacg gcagatagtg ctccggcgtt gggttcgata acgcgccacc
    44461 ttcatggtca aggtaattca ccagaggatg ctgctctacc gccccttgcc acgtcagatt
    44521 cgctttcaca tactcattaa acgacatcgc ccaaggatat ggtgaactat caccgtgcca
    44581 cttcactgta cgcaggttat gcaccacgtt gccactggcg accaacatta ttccttcatc
    44641 tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc
    44701 gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca gcacgcccca
    44761 ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg acgctaacag
    44821 ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg tatcgtacag
    44881 cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg cggtcactcc
    44941 tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc gtggcaatgt
    45001 catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact
    45061 accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa
    45121 aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca
    45181 atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt ataaggagtt
    45241 gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc
    45301 gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt cgctaggatt
    45361 tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg cactagcagc
    45421 tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc ttggctattt
    45481 tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa aatcggtgca
    45541 gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca tcagtctgca
    45601 taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg agctgggatt
    45661 tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag tggcaggccc
    45721 ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga tttctggact
    45781 agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga tttccccggt
    45841 ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga
    45901 attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg
    45961 tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa
    46021 acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt
    46081 ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca
    46141 gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca
    46201 ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt ttacccttaa
    46261 tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc ggatatattc
    46321 atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa aagaatgcct
    46381 gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc atagcagggc
    46441 aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt aattaagcag
    46501 cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt cttcaatagt
    46561 cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac gcgccatcgt
    46621 gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg ccagcgtcat
    46681 caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg cctgcgcgcg
    46741 aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat cggcaatcgc
    46801 tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac cttcagccgc
    46861 ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta ccatcattgg
    46921 cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac cgctaccgtg
    46981 gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga tcatatcgcc
    47041 ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac gcagcgcagc
    47101 cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg tctgattcat
    47161 ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta ggagtggcgg
    47221 catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact ctctcccatc
    47281 cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga gttcgcaccc
    47341 aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt tcgccccgcc
    47401 ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc atagcttcac
    47461 ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca cctatcagta
    47521 cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc aggttcacga
    47581 atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa tccgccaaac
    47641 cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg acgtccaaac
    47701 gcaagtcctg ttacggactc gtgagaaact ggcgcggctt gaacaaagaa tgagcgaact
    47761 ggaaaatcgc agtactgaga ttaaaaagca gccagatcca gagactctcc ccccaacgct
    47821 gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcttctaatt tacagtgctt
    47881 tctattacct tatcgcttaa ttccattcag aatatcatca caaaaatgaa tgagtgcaat
    47941 tgaaaatata attgttatgt attttttaag ttttacttaa tccatattta gataaactaa
    48001 tttcataaaa cccgaaaagt aaaatataat atttgaatga ttagcaatcc ttaaataaca
    48061 gttactaatc attcattttc tacttatatt tatataaagg aatattatgt ctgcttttaa
    48121 aaagtcactt cttgttgctg gcgtagcaat gattctttcc aataacgtat ttgctgacga
    48181 aggtcacggc attgttaaat ttaaaggtga agttatctct gctccatgtt caattaaacc
    48241 aggtgacgaa gacttaactg taaatctggg tgaagtagca gataccgttc tgaaaagcga
    48301 ccagaaatct ttggctgagc ctttcactat tcacctgcaa gactgcatgt tgagccaggg
    48361 tggaaccact tattcaaaag cgaaggttac tttcaccaca gcgaatacta tgacgggtca
    48421 gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag gcgttggcgt
    48481 acgtattctg gatagccaga gcggcgaagt gactttaggt actccggtag taattacatt
    48541 caacaatact aacagctatc aggaactgaa cttcaaagct cgtatggaat ccccgggcaa
    48601 agatgctacc ccgggcaacg tttacgctca ggctgactat aagattgctt acgagtaatc
    48661 acattaggcc gggttcgccc ggccacatca ttaacggatt aatgataagt ggaacagatg
    48721 tataaaaaat taaaattaac cacaataagc gaattgatta aaaatattta ttgttcatta
    48781 tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa agatttaatc
    48841 gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg ccaattaccc
    48901 gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat ccatctggac
    48961 ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga gcaattaaca
    49021 ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat cgatggttgt
    49081 taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa aatgcaatta
    49141 tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac gcctcctgaa
    49201 ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc ctctcattat
    49261 gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc tggggttaat
    49321 cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa caatggcaaa
    49381 agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat cccagcaatt
    49441 aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga ctctttccat
    49501 ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct acgtggttac
    49561 gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc acagaacaac
    49621 cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa tttattcaat
    49681 acattacagg ggcaactgga cgtcaaggtt gaagaagagg acgggccagt ctatatatca
    49741 atgacactaa ccattacatg aattatcaga atgaaaaaga gagttatatt gtcacgttta
    49801 accagta
//
LOCUS       DAJTYZ010000013        46160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000013 DAJTYZ010000000
VERSION     DAJTYZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.013"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..347)
                     /locus_tag="OO596_000763"
     CDS             complement(<1..347)
                     /locus_tag="OO596_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6716707.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVL"
     gene            418..1131
                     /gene="bdcA"
                     /locus_tag="OO596_000764"
     CDS             418..1131
                     /gene="bdcA"
                     /locus_tag="OO596_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6716708.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1262..1657
                     /locus_tag="OO596_000765"
     CDS             1262..1657
                     /locus_tag="OO596_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6716709.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            1938..2072
                     /gene="pyrL"
                     /locus_tag="OO596_000766"
     CDS             1938..2072
                     /gene="pyrL"
                     /locus_tag="OO596_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6716710.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2076..3011
                     /gene="pyrB"
                     /locus_tag="OO596_000767"
     CDS             2076..3011
                     /gene="pyrB"
                     /locus_tag="OO596_000767"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6716711.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3024..3485
                     /gene="pyrI"
                     /locus_tag="OO596_000768"
     CDS             3024..3485
                     /gene="pyrI"
                     /locus_tag="OO596_000768"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6716712.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            3558..3944
                     /gene="ridA"
                     /locus_tag="OO596_000769"
     CDS             3558..3944
                     /gene="ridA"
                     /locus_tag="OO596_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6716713.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4150..6846)
                     /gene="mgtA"
                     /locus_tag="OO596_000770"
     CDS             complement(4150..6846)
                     /gene="mgtA"
                     /locus_tag="OO596_000770"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6716714.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(6987..7040)
                     /gene="mgtL"
                     /locus_tag="OO596_000771"
     CDS             complement(6987..7040)
                     /gene="mgtL"
                     /locus_tag="OO596_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6716715.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7225..8172
                     /gene="treR"
                     /locus_tag="OO596_000772"
     CDS             7225..8172
                     /gene="treR"
                     /locus_tag="OO596_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6716716.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8291..9712
                     /gene="treB"
                     /locus_tag="OO596_000773"
     CDS             8291..9712
                     /gene="treB"
                     /locus_tag="OO596_000773"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6716717.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            9762..11417
                     /gene="treC"
                     /locus_tag="OO596_000774"
     CDS             9762..11417
                     /gene="treC"
                     /locus_tag="OO596_000774"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6716718.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVQHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            11811..13949
                     /gene="nrdD"
                     /locus_tag="OO596_000775"
     CDS             11811..13949
                     /gene="nrdD"
                     /locus_tag="OO596_000775"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6716719.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14107..14571
                     /gene="nrdG"
                     /locus_tag="OO596_000776"
     CDS             14107..14571
                     /gene="nrdG"
                     /locus_tag="OO596_000776"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6716720.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(14616..15002)
                     /gene="cybC"
                     /locus_tag="OO596_000777"
     CDS             complement(14616..15002)
                     /gene="cybC"
                     /locus_tag="OO596_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6716721.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15186..16538)
                     /gene="pmbA"
                     /locus_tag="OO596_000778"
     CDS             complement(15186..16538)
                     /gene="pmbA"
                     /locus_tag="OO596_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6716722.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            16632..17183
                     /locus_tag="OO596_000779"
     CDS             16632..17183
                     /locus_tag="OO596_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6716723.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17266..18639)
                     /gene="mpl"
                     /locus_tag="OO596_000780"
     CDS             complement(17266..18639)
                     /gene="mpl"
                     /locus_tag="OO596_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6716724.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            18815..19813
                     /gene="fbp"
                     /locus_tag="OO596_000781"
     CDS             18815..19813
                     /gene="fbp"
                     /locus_tag="OO596_000781"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6716725.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(19846..20841)
                     /gene="yjfF"
                     /locus_tag="OO596_000782"
     CDS             complement(19846..20841)
                     /gene="yjfF"
                     /locus_tag="OO596_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6716726.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(20828..21850)
                     /locus_tag="OO596_000783"
     CDS             complement(20828..21850)
                     /locus_tag="OO596_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6716727.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(21864..23366)
                     /locus_tag="OO596_000784"
     CDS             complement(21864..23366)
                     /locus_tag="OO596_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6716728.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(23506..24462)
                     /gene="ytfQ"
                     /locus_tag="OO596_000785"
     CDS             complement(23506..24462)
                     /gene="ytfQ"
                     /locus_tag="OO596_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR6716729.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            24772..25302
                     /gene="ppa"
                     /locus_tag="OO596_000786"
     CDS             24772..25302
                     /gene="ppa"
                     /locus_tag="OO596_000786"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6716730.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(25681..26022)
                     /locus_tag="OO596_000787"
     CDS             complement(25681..26022)
                     /locus_tag="OO596_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6716731.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26025..29804)
                     /gene="tamB"
                     /locus_tag="OO596_000788"
     CDS             complement(26025..29804)
                     /gene="tamB"
                     /locus_tag="OO596_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6716732.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(29801..31534)
                     /gene="tamA"
                     /locus_tag="OO596_000789"
     CDS             complement(29801..31534)
                     /gene="tamA"
                     /locus_tag="OO596_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6716733.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            31740..32378
                     /gene="msrA"
                     /locus_tag="OO596_000790"
     CDS             31740..32378
                     /gene="msrA"
                     /locus_tag="OO596_000790"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6716734.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            32701..34044
                     /locus_tag="OO596_000791"
     CDS             32701..34044
                     /locus_tag="OO596_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6716735.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34124..34330)
                     /locus_tag="OO596_000792"
     CDS             complement(34124..34330)
                     /locus_tag="OO596_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6716736.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            34655..35209
                     /locus_tag="OO596_000793"
     CDS             34655..35209
                     /locus_tag="OO596_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6716737.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35299..36210)
                     /locus_tag="OO596_000794"
     CDS             complement(35299..36210)
                     /locus_tag="OO596_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716738.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36422..37162)
                     /gene="cysQ"
                     /locus_tag="OO596_000795"
     CDS             complement(36422..37162)
                     /gene="cysQ"
                     /locus_tag="OO596_000795"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6716739.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37352..39295
                     /gene="cpdB"
                     /locus_tag="OO596_000796"
     CDS             37352..39295
                     /gene="cpdB"
                     /locus_tag="OO596_000796"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6716740.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39413..39793)
                     /locus_tag="OO596_000797"
     CDS             complement(39413..39793)
                     /locus_tag="OO596_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6716741.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            39882..40742
                     /gene="qorB"
                     /locus_tag="OO596_000798"
     CDS             39882..40742
                     /gene="qorB"
                     /locus_tag="OO596_000798"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6716742.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            40850..41815
                     /locus_tag="OO596_000799"
     CDS             40850..41815
                     /locus_tag="OO596_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6716743.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            41923..42585
                     /gene="ytfE"
                     /locus_tag="OO596_000800"
     CDS             41923..42585
                     /gene="ytfE"
                     /locus_tag="OO596_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6716744.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(42630..44042)
                     /gene="cycA"
                     /locus_tag="OO596_000801"
                     /gene_synonym="dagA"
     CDS             complement(42630..44042)
                     /gene="cycA"
                     /locus_tag="OO596_000801"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6716745.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44351..44971)
                     /gene="fklB"
                     /locus_tag="OO596_000802"
     CDS             complement(44351..44971)
                     /gene="fklB"
                     /locus_tag="OO596_000802"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6716746.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45190..45828
                     /gene="ytfB"
                     /locus_tag="OO596_000803"
     CDS             45190..45828
                     /gene="ytfB"
                     /locus_tag="OO596_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCR6716747.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(45812..>46160)
                     /locus_tag="OO596_000804"
     CDS             complement(45812..>46160)
                     /locus_tag="OO596_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6716748.1"
                     /translation="NPWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGK
                     KHNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETIL
                     SWLLVDPSSTAHE"
BASE COUNT        11335 a        12280 c        11517 g        11028 t
ORIGIN      
        1 agaaccatac agccagcgca gccgccgttt tggctatatc tgcgcgccgc ttcttttaat
       61 acctcagcca ggcactcccc taccggacga tcatcacgaa gaatatcggc aagcggaata
      121 gcttccgtac cgacatattc attgagtaca cggctaaata acccagcctt actgccaaaa
      181 gccgcgtaga ggctcggggg gttaatacca agataatcag taacttcagc aacactgaca
      241 gcatcgaaac ctttttggtg aaaaagcact tttgccgcag agactgcctg ctcaggagcg
      301 aaacgtctgg gacgacctgg aacgcgagat tgttttttag tgaccattac aaaacttgtt
      361 gacagaaagt taaaacagtt ttgtaatgca tgttacataa taaatcaagg agtccttatg
      421 ggcgctttta caggtaagac agttctcatc ctcggtggca gtcgtggtat cggtgccgct
      481 atcgtacgtc gtttcgtcac cgatggggcc aatgtacgat tcacctatgc ggggtcgaaa
      541 gatgccgctg aacacctggc acaaaagact ggagcgacag cagtattcac agatagtgct
      601 gacagagacg ctgtcattga tgtcgttcgt aagagcggcg cattggatat cctggtggta
      661 aatgcaggta ttggcgtctt tggcgatgcc ctggaattga atgccgacga tattgatcgc
      721 cttttcaaaa tcaatattca tgctccttat catgcctctg ttgaagccgc ccggcagatg
      781 cccgaaggcg ggcgcatctt aatcatcggc tccgtgaatg gcgatcgtat gcctgttgca
      841 ggcatggctg cttatgccgc cagtaaatct gccctgcaag gcatggcgcg cgagctggcc
      901 cgtgatttcg gaccgcgtgg gatcaccatc aacgtcgtcc agccagggcc aattgatacc
      961 gacgctaatc ccgccaacgg gccaatgcgc gatatgttgc atagttttat ggctatcaaa
     1021 agacatgggc aaccggaaga ggtcgctggt atggtcgcat ggttagcagg gccagaagcc
     1081 agttttgtta ccggcgcgat gcataccatt gatggcgcgt ttggcgcata accgactacg
     1141 ctcaattaag cccagccatt tcccatgatg tctgggtttt gtttactcac gtcgtccgct
     1201 aaaagcggct cctggtaaat ataaatcttc tgttcatgtc gtaaatcttg aggataacca
     1261 aatggtagaa agaaccgctg ttttccctgc aggccgacat tcactgtatg ctgaacatcg
     1321 ttattctgcg gctattcgtt ccggcgattt gctgtttgtt tccgggcaag taggaagtcg
     1381 agaggacgga acaccagaac ccgattttca gcaacaagtc agactggcat ttgataattt
     1441 gcatgcgacc ctggctgctg cgggatgcac ttttgacgat atcattgatg ttacgagctt
     1501 ccataccgat ccagaaaacc aatttgaaga catcatgacg gtgaaaaatg aaatatttag
     1561 cgccccacct tatccaaact ggacggcggt aggtgttaca tggctggcag gctttgattt
     1621 tgaaattaaa gtgatagcgc gcatccctga gcagtaagca atagtgttag ccgttcgttt
     1681 tcacactccg ccctataagt cggatgaatg gaataaaatg catatctgat tgcgtgaaag
     1741 tgaaaaagga aaaagcagga aatgtctgca attattgata ccgaacgacc gttcccctgc
     1801 agaatcacaa caaataaaaa tgcatgtatt ttgacttata attcaaataa accgtttgcg
     1861 ctgacaaaat attgcatcaa atgcttgcac cgcttctgac gatgagtata atgccggaca
     1921 atttgccggg aggatgtatg gttcagtgtg ttcgacattt tgtcttaccg cgtctgaaaa
     1981 aagacgctgg cctgccgttt ttcttcccgt tgatcaccca ttcccagccc ctcaatcgag
     2041 gggctttttt ttgcccaggc gtcaggagat aaaagatggc taatccgcta tatcagaaac
     2101 atatcatttc cataaacgac cttagtcgcg atgaccttaa tctggtgctg gcgacagcgg
     2161 cgaaactgaa agcaaacccg caaccagagc tgttgaagca caaagtcatt gccagctgtt
     2221 tcttcgaagc ctctacccgt acccgcctct ctttcgaaac ttccatgcac cgcctgggtg
     2281 ccagcgtggt gggcttctcc gacagcgcca atacatcact gggtaaaaag ggcgaaacgc
     2341 tggccgatac catttcggtt atcagcactt acgtcgatgc gatagtgatg cgtcatccgc
     2401 aggaaggtgc ggcgcgcctg gccaccgagt tttccggcaa tgtaccggta ctgaatgccg
     2461 gtgatggctc caaccaacat ccgacgcaaa ccttgctgga cttattcact attcaggaaa
     2521 cccaggggcg tctggacaat ctccacgtcg caatggttgg tgacctgaaa tatggccgca
     2581 ccgttcactc cctgactcag gcgttagcga agttcgacgg caaccgtttt tacttcatcg
     2641 cgccggacgc gctggcaatg ccgcaataca ttctggatat gcttgatgaa aaagggatcg
     2701 cctggagtct gcacagctct attgaagaag tgatggcgga agtagacatc ctgtacatga
     2761 cccgcgtgca aaaagagcgt ctggacccgt ccgagtacgc caacgtgaaa gcgcagtttg
     2821 ttcttcgcgc cagcgatctc cacaacgcca aagccaatat gaaagtgctg catccgctgc
     2881 cgcgtgttga tgagattgcg acggatgttg ataaaacgcc acacgcctgg tacttccagc
     2941 aggcaggcaa cgggattttc gctcgccagg cgttactggc actggttctg aatcgcgatc
     3001 tggtactgta aggggaaata gagatgacac acgataataa attgcaggtt gaagctatta
     3061 aacgcggcac ggtaattgac catatccccg cccagatcgg ttttaagctg ttgagtctgt
     3121 tcaagctgac cgaaacggat cagcgcatca ccattggtct gaacctgcct tctggcgaga
     3181 tgggccgcaa agatctgatc aaaatcgaaa ataccttttt gagtgaagat caggtagatc
     3241 aactggcatt gtatgcgccg caagccacgg ttaaccgtat cgacaactat gaagtggtgg
     3301 gtaaatcacg ccccagtctg ccggagcgca tcgacaatgt gctggtctgc ccgaacagca
     3361 actgtatcag ccatgccgaa ccggtttctt ccagctttgc cgtgcgaaaa cgcgccaatg
     3421 acatcgcgct caaatgcaaa tactgtgaaa aagagttttc ccataatgtg gtgctggcca
     3481 attaattgcg gttggtaata aaagtctggc tccctataat gagccagact ttttaccgct
     3541 gtaataaagg agaaatcatg agcaaaacta tcgcgacgga aaatgcaccg gcagctatcg
     3601 gtccttacgt acagggcgtt gatctgggca atatgatcat cacctccggt cagatcccgg
     3661 taaatccgaa aacgggcgaa gtaccggcag acgtcgctgc acaggcacgt cagtcgctgg
     3721 ataacgtaaa agcgatcgtc gaagccgctg gcctgaaagt gggcgacatc gttaaaacca
     3781 ccgtgtttgt gaaagatctg aacgacttcg caaccgttaa tgctacctac gaagccttct
     3841 tcaccgaaca caatgccacc ttcccggcac gttcttgcgt tgaagttgcc cgtctgccga
     3901 aagacgtgaa aattgagatc gaagcgatcg ctgttcgtcg ctaatcttga tggaaatccg
     3961 ggctatcatg cccggattaa gtctgatgac aaacacaaaa tcgcctgatg cgctacgctt
     4021 atcaggccta cgtgattcct gcaatttatt gaatttgttg gccggataag gcatttacgc
     4081 cgcatccggc atgaacaaaa ctcactttgt ctgcaatctg aatccaggcc accatgccca
     4141 gttgattctt tattgccagc cgtaacgacg gctataaaat cctttcacca gttgggttaa
     4201 cgtcatatac cctgccagaa tcgcaaccag ccacgggaaa tagcttaacg gcagcgcctg
     4261 cagttgcaga taactggcca gcggtgaaaa cggcaatgcg atcccgacaa tcatcacgat
     4321 cacggtcatg atcattaacg gccacgatgc acagctctga ataaacggca cacggcgggt
     4381 gcggatcata tgcacaatca gcgtttgcga cagcaagccc accacaaacc atcccgactg
     4441 gaacagcgtt tgcgtttccg gcgtgttggc atggaatacc caccacatca ggcaaaacgt
     4501 cagaatatcg aagatcgagc tgatcggtcc gaagaagatc ataaagcgcc ccagatccgc
     4561 cggattccaa cgctgcggct tttgaatttg ctcgtcgtcg acgttatcaa acgggatcgc
     4621 cacctgtgac acatcataca gcaggttctg aataagcaag tgtaacggca acatcggcag
     4681 gaagggcaag aaagcactcg ccaccagcac gctgaacaca ttaccgaagt tggagctcgc
     4741 cgtcattttg atgtatttca gcatgttggc gaaagtgcga cgtccttcaa taaccccctc
     4801 ttccagcacc atcaggcttt tttccagcag gatgatatca gccgcttcac gggcaatatc
     4861 taccgcgccg tccacagaaa tgccgatatc cgcagcgcgt aaggcgggcg catcattaat
     4921 accatcgccc ataaagccaa ccacatgccc ttcgcgcttc agtaaggtca caatgcgttc
     4981 tttatgcatc ggcgtcagac gagcaaacag cgtggtgcgc tgtgcgagat ttgccagttc
     5041 gtcgtcagat agcgtttcaa tatcactacc aatgaccacc tctcccgcgt ccagccccac
     5101 ttcatggcac actttcgctg caactaactc actgtcgcca gtaaggattt ttacggtaat
     5161 tccactcgct tttaatgcct tcagtgccgg agcagttgtc tcttttggcg gatcaaggaa
     5221 agcaatatat ccttcgagga tcaggtcgga ttcatccgcc cgctggtaat ctccttcacg
     5281 cgctggcagg tatttcgtcg ccaccgcaac cacgcgcagc ccctgacgat tcagcgtatc
     5341 agtaacccgc ttaatcttac gcagcatgat gtcatcgagc ggcacaatct cgccattgtg
     5401 acgcacctgc gaacacacat tgaggatttc ctgcaatgca cctttgcaaa ccagctgatg
     5461 gtgctcggta ttttctgcca ctaccaccga catccggcga cgctcgaaat caaacggaat
     5521 ctcatcaatt ttttgccaac ggctggccag agagcgcgct gactcttcat ccgtaccttc
     5581 gagcaccgct gtatcgagta ggtttttaag tccggtctga taatgactgt tcaaccacgc
     5641 gctatgcagc acgcgttcgc tggttttacc ggagatatcg gtatgattct ccagcacaat
     5701 tttgtcctgt gtcagggtgc cagttttatc agtgcacaga atatccattg cgccaaagtt
     5761 ctgaatagca tccagatgtt tgacgatcac tttctgtttc gacagcttta ctgcgccgcg
     5821 cgccagcgtc gaggtaacaa tcatcggcaa catttccggc gttaagccta ccgcgaccga
     5881 aagcgcaaac agtgccgctt cccaccagtc gcctttggtg taaccgttga taagcaacac
     5941 caccggagcc atcaccagca taaagcgaat cagcagcata ctgacgcggc tgatcccctg
     6001 ctgaaaggca ttcggctcgc tttcctgctc actaacacgc cccgccagtt gaccaaacca
     6061 ggtgttggct cctgtagcaa tcaccattgc ttgtgccgtg ccgctcacca cggtggtgcc
     6121 cataaaacac agggtgtcac actccagcgg attgctgtgc tccggctggc gagtggtagc
     6181 ggctttttcc acgggcagag actcaccggt taacgacgcc tgagcgacga acagatcccg
     6241 cgcctgcaag atacgtaaat ctgccgggat catatccccc gccgccagtt ttataatatc
     6301 gccgggcacc agctggtcga tcgggatctc cagccagcca ttttcgcctt tgtcgttaat
     6361 tacgcgcagc accgtcgcgg tattgctgac catcgctttc agggcatctg ccgctttagt
     6421 ggaacgtgct tcctgaataa agttcagcaa cgtagaaata gcgaccatta gcgcgataac
     6481 gcccgcggca aataaatctt cagtggcgta agaaatagcg ccgagaatgg tgagtaaaat
     6541 attaaatggg ttgcgatagc agacccataa atgtacccac cacggcgacg gttgttgtgc
     6601 gggtaattta ttttcaccat gttgttcgcg ggcagattcc acttccgcct gatttaaccc
     6661 ttccggatgg gtgtcgaacg ttttccacaa ttcttcttcg ggcatcaccg ccattttcag
     6721 gcaatgcgca cttaaggacg gcgggaccgc cgtattcact gtctgctgcg cgccaggcaa
     6781 tggatcgcga tgaaccagac gggaaggtaa atggcgaatg agccgggtaa aaatttcttt
     6841 aaacataagg agtccctccg cactgtctga aacagtacag aaatgtcttc aggcgtggcg
     6901 aagccttacc tgaaggtaat aaagctgagc agggacgtta aaacgtcacg gccttacgca
     6961 tccggtagcg ccgtgaaaag acgggcttac cggaaaagtt ttaatctccg tcgagggaga
     7021 ggcgtgggat caggttccat ataacctccg gtaagtgaaa aaagcgccgc aaataataag
     7081 gagaatccaa taatattcgt agcatttatg gcgatataat acttgctggc aactaaacca
     7141 acgataaacc agactttacc cattgctgaa tgcacgggta aagttaggct caaataatta
     7201 aacaacacgt tacaggacaa caggatgcaa aatcggctga ctatcaaaga catcgcacgc
     7261 ttaagcggcg tggggaaatc tacagtttcc cgggtgctga ataacgaaag cggcgtgagc
     7321 cagcgcaccc gcgagcgtgt tgaagcagtg atgaatcagc atggattttc cccttcccgc
     7381 tctgcgcgcg ctatgcgtgg gcaaagcgat aaagtggtcg ccatcattgt tacccgtctg
     7441 gattcgttgt cagaaaatct cgccgttcaa accatgctgc cagcgttcta tgaacaaggt
     7501 tacgacccaa tcatgatgga aagccagttt tccccgcaat tagttgccga acatttgggg
     7561 gtgctgaaac ggcgtaatat cgacggcgta gtgctgttcg gttttactgg cataacagaa
     7621 gaaatgttag cccactggca gtcatcgctg gttctgctgg cgcgtgacgc aaaaggcttt
     7681 gcttcggtct gttatgacga cgaaggggca atcaagatcc tgatgcaacg gctgtatgac
     7741 caggggcatc gtaatatcag ttatctcggc gtgccgcata gtgacgtgac aaccggtaag
     7801 cgacgtcacg aagcctacct ggcgttctgc aaagcgcata aactgcatcc cgttgccgct
     7861 ctgccagggc ttgctatgaa gcaaggctat gagaacgtgg caaaagtgat tacgcctgaa
     7921 actactgcgt tactgtgcgc aaccgacacg ctggcacttg gcgcaagtaa atacctgcaa
     7981 gagcaacgca tcgacacctt gcaactggcg agcgtcggta atacaccgtt aatgaaattc
     8041 ctccatccgg agatcgtcac cgtagatccc ggttacgccg aagctggacg ccaggcggcc
     8101 tgccaattga tcgcacaggt aaccgggcgc agcgaaccgc aacaaatcat catccccgcc
     8161 accctgtcct gatcgtttcc tgaacgataa attgtgatct tcgctgcatt tcgggaacgt
     8221 tcccgttttt aaatttttcc gcgcaatata ttctgcagcc aaccaaaaat gtcatctgcc
     8281 atggggcttt atgatgagca aaataaacca aacggatatc gatcggttga ttgaactggt
     8341 cggcgggcgc ggcaatattg cgacggtgag ccactgtatt actcgcctgc gctttgtcct
     8401 caaccaaccg gccaatgcca gaccgaaaga aattgagcaa ctccccatgg tgaaaggctg
     8461 tttcaccaat gccgggcaat ttcaggtggt gattggcacc aacgtgggtg attactatca
     8521 agcactaata gcgtcaaccg gacaggcgca ggttgataaa gagcaggtaa aaaaagccgc
     8581 ccggcagaat atgaaatggc atgagcagtt gatctctcat ttcgcggaga tcttcttccc
     8641 gttgctgccc gcgttgatta gcggcggttt gatcctcggt tttcgcaatg tgatcggcga
     8701 tttgcccatg agcaacggtc agacgctggc gcaaatgtac ccttccctgc aaacgatcta
     8761 cgattttctg tggttgatcg gtgaagcgat tttcttctac ctgccggtcg ggatttgctg
     8821 gtcagcggtg aaaaaaatgg gcggcacgcc gatccttggt atcgtgcttg gcgtgacact
     8881 ggtttccccc cagctgatga acgcttatct gctcgggcag cagctgccgg aagtgtggga
     8941 ctttggcatg ttcagcatcg ccaaagtggg ctatcaggcg caggtgatcc cggcactgtt
     9001 agccgggctg gcgctgggcg ttattgaaac tcgccttaaa cgcatcgtac cggattacct
     9061 ctatctggtg gtggtgcccg tctgttcgct gatcctcgcg gtgttcctcg cccatgcgct
     9121 gattggtccg tttggtcgca tgattggcga tggcgttgcc tttgcggtac gtcacctgat
     9181 gaccggcagc tttgctccga ttggtgcggc attgtttggc ttcctgtacg cgccgctggt
     9241 gatcaccggc gtacaccaga ccacccttgc tattgatttg cagatgattc aaagcatggg
     9301 tggcacgcca gtgtggccgc tgattgcgct gtcgaatatc gctcagggct ccgccgtgat
     9361 aggcattatc atttccagcc gcaagcacaa tgaacgcgag atctccgtgc ctgccgctat
     9421 ctccgcctgg cttggggtca ctgagcctgc aatgtacggc atcaacctga aatatcgctt
     9481 cccgatgctg tgcgcgatga ttggttctgg tctggcagga ttgctatgcg gcctgaacgg
     9541 cgttatggcg aatggtatcg gcgtaggggg cctgccggga attctctcga ttcaaccgag
     9601 ctactggcag gtatttgcgc tggcaatggc tatcgccatc atcatcccga ttgtactcac
     9661 ctcgtttatc tatcagcgga aataccgctt gggcacgctg gacattgttt aattttcttc
     9721 ggggcgcaat tgcgccccct cgcatttgca ggaataacgt aatgactaat cttccccact
     9781 ggtggcaaaa cggcgttatc taccagattt atccaaagag ttttcaggac accacgggta
     9841 gcggcaccgg cgatttacgt ggcgttatcc aacgcctgga ctatctgcat aaactgggcg
     9901 ttgatgccat ctggctaacc cccttttatg tctctcccca ggtcgataac ggttacgatg
     9961 tagcgaacta tacggcgatt gatcccacct acggcacgct ggacgatttt gacgaactgg
    10021 tgacgcaggc aaaatcgcgc gggattcgta tcattctcga tatggtgttt aaccatacct
    10081 ctacccaaca tgcctggttt cgcgaggcgc tgaacaaaga aagcccttac cgccagtttt
    10141 atatctggcg cgatggcgaa ccagaaacgc caccgaacaa ctggcgttca aaatttggcg
    10201 gtagtgcgtg gcgctggcat gcggaaagcg aacagtacta tttgcatctc tttgcaccag
    10261 aacaggcgga tctcaactgg gagaatccag cggtacgcgc agagctgaaa aaagtctgtg
    10321 aattctgggc cgatcgtggg gtcgacgggt tgcgcctgga tgtggtgaat ctgatctcca
    10381 aagacccgcg tttccctgaa gacctggatg gcgacgggcg tcgcttctac accgacgggc
    10441 cacgagcaca cgagtttttg cacgagatga accgcgatgt gtttacgcca cgcgggttaa
    10501 tgaccgtagg tgaaatgtcc tccaccagcc ttgagcattg ccagcgatac gcggcactga
    10561 caggcagtga attgtcgatg acctttaatt ttcatcacct gaaggtcgat tatcccggtg
    10621 gtgaaaaatg gacgctggct aaacctgact ttgtggcgtt gaaaacattg ttccgccact
    10681 ggcaacaagg aatgcacaac gtggcatgga atgccttgtt ctggtgtaac cacgatcagc
    10741 cgcgcattgt ttctcgcttt ggtgatgaag gtgaataccg cgtgcctgcg gcaaaaatgc
    10801 tggcgatggt gcagcatggc atgcagggaa cgccgtatat ctaccagggc gaagagattg
    10861 gcatgaccaa cccgcatttc acgcgcatta ctgactatcg cgacgtggag agcctcaata
    10921 tgtttgccga gctgcgcaac gatggtcgtg atgccgacga gttattggca atcctcgcca
    10981 gtaaatcccg tgacaacagc cgcacgccca tgcaatggag caacggcgat aatgccgggt
    11041 ttacggctgg cgaaccgtgg attggcctgg gcgataacta tcaacaaatc aacgtagaag
    11101 ccgcgctggc cgatgactcc tcggtgtttt acacctacca aaagttaatc gcactgcgta
    11161 agcaagaagc catcctgaca tggggcaatt accaggatct gctgccaaac agccctgtat
    11221 tgtggtgcta tcgccgtgaa tggaaggggc aaaccttgct ggtcattgcc aaccttagcc
    11281 gtgagatcca accctggcag ccagggcaaa tgcgcggcaa ctggcagctt gtgatgcata
    11341 actacgaaga agcctcacca caaccctgtg ccatgaattt acggcctttt gaggctgtct
    11401 ggtggttaca gaagtaaatc ttccctaagc cccggtaatg ccggtcattc cggggttttg
    11461 tctgtcagca tgttgttttt gttgattttt caaccagcaa attcattaaa aaatttacat
    11521 aacgctgtag cgcccgtcat ccgtacgctc tgctttttac tttgagctac atcaaaaaaa
    11581 gttaaaacat ccttgatgca aagcactata tatagacttt aaaatgcgtc ccaacccaat
    11641 atgttgtatt aatcgactat aattgctact acagctcccc acgaaaaagg tgcggcgttg
    11701 tggataagcg gatggcgatt gcggaaagca ccggaaaacg aaacgaaaaa accggaaaac
    11761 gcctttccca atttctgtgg ataacctgtt cttaaaaata tggagcgatc atgacaccgc
    11821 atgtgatgaa acgagacggc tgcaaagtgc cgtttaaatc agagcgcatc aaagaagcga
    11881 ttctgcgtgc agctaaagca gcggaagtcg atgatgccga ttattgcgcc actgttgccg
    11941 cggttgtcag cgagcagatg cagggccgca accaggtgga tatcaacgag atccagaccg
    12001 cagttgaaaa ccagctgatg tcgggtccat acaaacaact ggctcgtgct tacatcgagt
    12061 accgtcacga tcgcgacatt gaacgtgaaa aacgcggtcg cctgaaccag gagatccgtg
    12121 gtctggtcga gcagaccaac gcctcgttac tcaacgaaaa cgccaacaaa gacagcaagg
    12181 tgattccaac ccagcgcgac ctgctggccg ggatcgtggc taaacactat gcacgccagc
    12241 acctgctgcc gcgtgacgtg gtgcaggcac atgagcgtgg cgatattcac tatcacgatc
    12301 tcgattactc gccgttcttc ccgatgttca actgcatgtt gatcgacctg aaaggcatgc
    12361 tgacccaggg ctttaaaatg gggaacgccg agattgaacc gccgaagtcg atctctacgg
    12421 caaccgcggt aactgcgcag attattgctc aggtcgccag ccatatttat ggcggcacca
    12481 ccattaaccg tatcgatgaa gtgctggcgc cgtttgttac cgccagctac aacaaacatc
    12541 gcaagaccgc agaagagtgg aacatcccgg acgccgaagg ctacgctaac tctcgtacca
    12601 tcaaagagtg ctacgatgcc ttccagtcgc tggagtacga agtaaacacc ctgcacaccg
    12661 ccaacggtca gacgccgttt gtaacctttg gttttggtct gggcaccagc agggaatcgc
    12721 gcctgattca ggaatcgatc ctgcgtaacc gtatcgcagg cctcggtaaa aaccgtaaaa
    12781 ctgcggtgtt cccgaaactg gtgtttgcga ttcgcgatgg cctgaaccat aaaaaaggcg
    12841 atccgaacta cgacatcaaa cagctggcgc tggagtgcgc aagcaagcgc atgtatccgg
    12901 atatcctgaa ctacgatcag gtagtgaaag tcaccggttc gtttaaaacc ccgatgggct
    12961 gccgcagctt cctcggcgtg tgggaaaatg aaaacggcga gcagatccac gatggtcgta
    13021 acaacctcgg cgtgatcagc ctgaacctgc cgcgtattgc tctggaagca aaaggcgatg
    13081 aagccacctt ctggaagctg ctggatgaac gtctggtgct ggcacgtaag gcactgatga
    13141 cccgtatcgc tcgtctcgaa ggcgtgaaag cgcgcgtcgc cccgatcctc tatatggaag
    13201 gtgcttgtgg cgtacgtctg aatgctgacg atgatgtttc tgaaatcttc aaaaacggtc
    13261 gtgcgtctat ttcgctgggt tacatcggca tccacgaaac cattaacgcg ctgttcggcg
    13321 gcgagcatgt ttacgacaac gagcagcttc gcgcgaaagg tatcgccatt gttgaacgtc
    13381 tgcgtcaggc agtggatcag tggaaagaag aaacgggtta tggtttcagt ctctacagca
    13441 cgccgagtga aaacctgtgc gaccgcttct gccgtctcga tactgctgag tttggcgtgg
    13501 tgccgggcgt gaccgataaa ggttactaca ccaacagttt ccaccttgat gtggagaaga
    13561 aggtgaaccc gtacgacaag atcgactttg aagcgcctta cccgccgctg gcgaacggtg
    13621 gtttcatttg ctacggcgag tatccaaaca ttcagcacaa cctgaaagcg ctggaagatg
    13681 tctgggatta cagctatcag catgtaccgt attacggcac caatacaccg attgatgagt
    13741 gctacgagtg tggctttacc ggtgagttcg agtgcaccag caaaggcttc acttgcccga
    13801 aatgtggtaa ccatgacgcc tcccgtgtgt cggtaactcg ccgcgtgtgc ggatatttag
    13861 gtagcccgga tgcgcgtccg tttaacgccg gtaagcagga agaagttaag cgccgcgtta
    13921 aacatttggg gaatgggcag ataggttaat cttcactctg cgttaagcaa atgccggatg
    13981 cggcgtaaac gccttatccg gcctacattt ggcaacggct gtaggcctga taagacgcga
    14041 cagcgtcgca tcaggcattg attaccggat gcagcctaca cgcggactga taagccaggc
    14101 gaaataatga attatcatca gtactatcct gtcgacatcg tcaacggccc cggcactcgc
    14161 tgcaccctgt ttgtctccgg gtgtgttcat gaatgccccg gttgctataa caaaagcaca
    14221 tggcgggtaa attctggtca gccatttacc aaagcaatgg aagacaagat cattaaagat
    14281 ctgaatgaca ctcgtatcaa acgccagggg atttccctct ccggcggcga tccgctgcat
    14341 ccgcaaaacg tgccggatat tctgaaactg gtaaaacgca tccgcgccga gtgtccgggt
    14401 aaagacatct gggtgtggac aggctataaa ctcgacgaac tcaacgctgc gcaaatgcag
    14461 gttgttgatt tgattaacgt gctggtcgac ggcaaatttg tgcaggattt aaaagaccct
    14521 tccctgatct ggcgcggcag cagcaatcag gtggtgcatc atttgcgatg ataagttccc
    14581 tgttacctga gtgcaggcaa cagggaaatg aggaattaac gatacttctg gtgataggcg
    14641 ttgcgggtcg ttttcagttg ctctgcagca gcctgcgctt cttttacttt accttcattt
    14701 gccagcttca gcgcgtcgtc aatctgaccg accagaatgt cgaaaccgtg gcggaaatct
    14761 ttcatttccg ggctgtccgg tgatttatct tcgagcttcg gcggcgttgc tttttgcgca
    14821 tccagcgctg cggcgcgcat cttcgttaac gcgtctttga cttgcgtcgc gttatcggct
    14881 ttttcgacca cttttaaatt gtcgttgagg gtttccatat tgtcttcaag atcagcggca
    14941 aacgacgccg aactgaatac caacgaggag actgcaagaa tagctaacag gcttttacgc
    15001 attgctcact tcctttttta tttacacttc atccttcaca ctgcctcatc gacagctacg
    15061 ctcattcgac cgggtcacgt actacgagta cgctccccgg ctctcattca ctttccgtct
    15121 cgatgcagtg cgaatgattt tgtgtaattt tttagtttat agcgcggcag gtcgcgccag
    15181 tttttttact gtccggcgat tttcatctcc ggcaacagca cagaaccaca ctgtatatta
    15241 ctgcgtgttt caatatcgtt accgacggtg acaatattgc gccacatatc ttttaaatta
    15301 cctgcgatgg tgatttcgct caccggatac tgaatttcgc cattctctac ccagaagccc
    15361 gcagcaccac gggaataatc accggtaatg gcactcacgc cctggcccat cagctccgtc
    15421 accaccagcc cggtgcccat ctctttgagc atttgttcga agcttagccc ttgtccggca
    15481 atccgccagt tgtgaatacc gcccgcatgt ccggtgcttt tcagcccaag ttttcgcgcc
    15541 gaatagctgg tcagcagcca ctgagtcagg atgccatctt taataatatc gcgacgctcg
    15601 gtgcgcacac cttcgctgtc gaatggcgtc gacgccagcc ctttcagcag atgcggatgc
    15661 tcttcaatgg tcagccagtc cggcagaatt tgtttaccca gcgaatccag caggaaggta
    15721 gatttacgat aaaccgatcc acccgctatc gcccccacca gatgcccaaa aagcccagtt
    15781 gccacttcat tggcaaaaat gactggcgct ttcatggtgg agagtttacg cggtaacagg
    15841 cgcgataaag tacggcgagc acagtcggcc ccaacccact ctggcgtttg cagatcgctc
    15901 atcgcgcgcc caatggtgta ggcgtaatca cgctccatat cgccattttc ttcggcaatt
    15961 acacagctgg agagcgaatg acgcgttgag cagtaaccct gcaacatgcc gtggctgttg
    16021 ccaaaaactt tgacaccgta gtggctgtta aagctgccac cttcggtatt ggtgatacgt
    16081 ttatccgcct gtaatgctgc ctgttctgcg cgggccgcca gttcaatggc ttcatccggg
    16141 gaaacttccg cagggtggaa caagtcgaga tctggtgcgt caaaggccag cagctctttg
    16201 tctgccaggc cggcacaggg atctggcgag gtataacggg caatatccag cgccgcctgt
    16261 acagtacggg caatggcctg cgggcttaaa tcggtggatg atgcgctacc tttgcggttc
    16321 tggtgataaa cagtgatccc cagcgcgcca tcgctattga attcaacatt ctccacttca
    16381 ccataacgcg tgcttacgct aatgccggtg gtcttgctga cggcaacttc cgcaccgtcc
    16441 gatttgcctg aggccaactc cagcgcagtg gaaactgctt cttccagaat cttgcgctgc
    16501 gcttcaactt gagagattac tttcattgca agtgccataa tgtagagagt ttctctgaag
    16561 tctaacagag aaccgttttt cagtgcgcat cttaactggt aacattagcc tcttttttta
    16621 aggagcctga gatgactaag cagcccgaag actggctcga cgacgttccc ggtgatgaca
    16681 tcgaagacga agacgatgaa attatctggg tcagtaaaag tgaaattaaa cgtgatgccg
    16741 aggagctaaa acgccttggc gcggaaattg ttgatctggg gaaaaacgcg ctggataaga
    16801 tcccgttaga tgcggatctg cgcgcggcta ttgagctcgc ccagcgtatt aagatggaag
    16861 gtcgccgccg ccagttgcag ctcatcggta agatgctgcg ccagcgcgac gtagagccta
    16921 ttcgtcaggc gctggataag ctgaaaaacc gtcacaacca gcaggtggtg ctgttccata
    16981 aactggaaaa cctgcgcgat cgtctgatcg atcaaggtga tgacgccatc gctgaagtat
    17041 tgaatctgtg gcccgatgcc gatcgtcagc aactgcgtac actgatccgc aacgcgaaga
    17101 aagagaaaga agggaataag ccgccgaaat ccgcacgcca gattttccag tatctgcgcg
    17161 aactggcgga gaatgaaggg taattttcgt tgttgatgtg agtatggttt gccggatgcg
    17221 gcgtaaacgc cttatccggc aatgctatat caggctgaag ccgaattact gcgcagcttc
    17281 cgccttcttc gccagaccat ccagcagttt ctgatggatc ccaccaaaac cgccgttgct
    17341 catcaccaga atatggtcgc caggctgagc ggttttcacc accatatctg ccagcgtatc
    17401 cacatcgcca ctccagtgtg caggctgaac gcaggcttct gccacttctg ccacctgcca
    17461 cggaatatgc gccggttgca gcaggaagac ttcatcggca cgacctaatg aaggtgccag
    17521 atcgtctttg cagatcccca ttttcatggt attcgagcgc ggttccagca cagcaataat
    17581 gcgcgccgta ccaccaactt tgccacgcag cgccgccagc gttgccagaa tcgccgtcgg
    17641 gtgatgggca aaatcgtcat ataccgtgac gccattcgct tcaccacgca actccagacg
    17701 gcgacgagca ttaataaacg aacccagcgc gttagcggca tctgccgacg ctacaccaac
    17761 atggcgagcc gctgcaatcg ccatcaggcc attgtgcata ttatgttcgc ctaccagcga
    17821 ccatttcact tcgcccactt tttcgccatc cagcaaaact tcccattcgg aagcatcggt
    17881 ggtcagcttt ttcgcctgcc agtgaccctg ctcacccacc agctcctgct cgctccagca
    17941 gcccatcgcc atggtctgtt tcaggttgat gtcattttcc ggccagataa tacggccctg
    18001 ccccggaacg atacgcacca gatggtggaa ctgtttctgg atcgctttca ggtcgtcaaa
    18061 gatatcggcg tgatcgaact caaggttgtt gaggatcagc gtacgcgggc agtaatggac
    18121 aaatttagag cgtttgtcga agaaggcgca gtcgtactca tccgcttcga taacaaagaa
    18181 gtcgctttcg cccagacgcg ccgaaacctc aaagttcccc ggcacaccgc cgatcacaaa
    18241 tcccggtttg taaccacact gttccagaat ccaggtcgcc attcccgcgg tggtggtttt
    18301 gccgtgtgta ccggcaacgg ccagcaccca gcggtcgcgc agcacaaaat cgtgcagcca
    18361 ctgtggacct gacatataag ggatgttttt ttccagtacc gcttccacac acggatttcc
    18421 acgggtcatg gcgttgccaa taatcaccag atccggctgc ggctcgagct ggctggcatc
    18481 gtaaccctga atcagctcaa tgccttgctt ctcaagtaag gtgctcatcg gcggatacac
    18541 attggcgtcc gaacccgtta cttcatggcc taactggcgc gccagcatcg ccagaccgcc
    18601 cataaatgtg ccacaaattc ctaaaatatg aatgcgcata cgtcactatc cttttttaat
    18661 ctgccgctca ttttacgcat atgtcgggca agtgagaaac gcatttcagg ataatccgca
    18721 atttgctggc gcgattcacc tgagcgcaac attgagatta tttgttaaga ttgttgcggt
    18781 cgctttactc cataaacatt gcagggaaag ttttatgaaa acgttaggtg aatttattgt
    18841 cgaaaagcag cacgagtttt ctcatgctac cggtgagctc actgctttgc tgtcggcaat
    18901 aaaactgggc gccaagatta tccatcgcga tatcaacaaa gcaggactgg ttgatatcct
    18961 gggtgccagc ggtgctgaga acgcgcaggg cgaggttcag caaaaactcg acttgttcgc
    19021 taatgaaaaa ctgaaagccg cactgaaagc acgcgatatc gttgcgggga ttgcctctga
    19081 agaagaagat gagattgtcg tctttgaagg ctgtgaatac gcaaaatacg tggtgctgat
    19141 ggaccccctg gatggctcgt ccaacatcga tgttaacgtc tctgtcggta ccattttctc
    19201 catctaccgc cgcgttacgc ctgttggcac gccggtaacg gaagaagatt tcctccagcc
    19261 tggtaacaaa caggttgcgg caggttacgt ggtatacggc tcctctacca tgctggttta
    19321 caccaccgga tgcggtgttc acgcctttac ttacgatcct tcgctgggcg ttttctgcct
    19381 gtgccaggaa cggatgcgct tcccggagaa aggcaaaacc tactccatca acgaaggaaa
    19441 ctacattaag tttccgaacg gggtgaagaa gtacattaaa ttctgccagg aagaagataa
    19501 atccaccaat cgcccgtata cctcgcgtta tatcggttca ctggttgcgg atttccaccg
    19561 taacctgctg aaaggcggta tttatctcta cccaagcacc gccagccacc cggacggcaa
    19621 actgcgtttg ctttatgagt gcaacccgat ggcattcctg gcggaacaag cgggcggtaa
    19681 agcgagcgat ggcaaagagc gtattctgga tatcatcccg gaaaccctgc accagcgccg
    19741 ttcgttcttt gtcggcaacg accatatggt tgaagatgtc gaacgcttta tccgtgagtt
    19801 cccggacgcg taatctcctg cgcctctcca cacggagagg cgtttttatt gctgcgcaat
    19861 gttgactctt gtcactggcg aactctgacg attctcccac agcaccgtta atccacgctg
    19921 taatgctata aaaataaaca acaaaatacc gatggcgatt ttcgtccacc aggaactcag
    19981 cgtgccatca aagtttatgt aagtctgaat cagtccctga atcgccacgc caaaaagcgt
    20041 ccctaatacc gttccaacgc caccgctcaa aagcgtaccg ccaattacca ctgaggcgat
    20101 agcatccagc tccacaccta cgcccgccag cgcatatccg gcctgggtat aaatcgagaa
    20161 gacaatcccc gccagcgttg ccagtccggt ggagagcata taaatgcgaa tagtggtgct
    20221 gcgagtggaa atccccatca gattcgccga cgttgcgttg ccgccaatgg cgtatacctg
    20281 attaccaaaa cgggtacgat gcgcgaggaa tatgccgata accaccaccg ccaacatcag
    20341 cagtcccatc gcacttaagc gaccgccgcc agggattttc cacgcaaggc ttgagagcgt
    20401 gtcataaatg ggatggttta tcggaatcga ctcttccgaa acgagatagc tgacgccgcg
    20461 caaaaagaac atccccgcaa gcgtaatgat gaacgccggg atcttcaggg cgtcgatcag
    20521 aagccccata aatgcgccga aggcacagcc catcaccagc accagcggaa acgccagcag
    20581 cggcgagagg ccgaaatcgc caatcacttt tgccagaaac acgccggtaa aggcgatcac
    20641 cgaaccgacg gagagatcga tcccaccgga gaggatcaca aaggtcatgc caacggcaat
    20701 gatcccaaga aaggcgttat cggtcaggat attgcagatc actcttgtgg aagcaaaacc
    20761 aggaaactgc gtcaggcagt agagatagcc cagcacaaag acgccgatgg tgatcataag
    20821 cggcaagtta cgttttatca cggctacgca ctcctttaat cagactgata aagcgttgcg
    20881 actgaacaat cagcacacaa agcaccacca ccgctttcac cacctggttc atctctggcg
    20941 gaaagcccga aagcaaaatt ccggtattca ttccctgaat aatcagcgcc ccaactcccg
    21001 aaagcagcag gttaaagcga ccgcccatca gcgatccacc gccaatcacc accgcgagaa
    21061 tggcatccag ctccagccat aatccggcgt tgttggcatc ggcaccgcga atatccgccg
    21121 ccacgataat gcccgcaatc gccgcacata gcccgctcaa cacgtaggtg agcatgacga
    21181 tgattcgtgt gttgactccg gcattttttg ccgcccgaat gttgatacca acggcttcga
    21241 taaacatccc cagcgccgtt ttgcgggtca acagccagaa caggagaagc gtcagcaccg
    21301 cgataatgac cggcgttggc aggaacaaca gcgatccgct gccaaaccat gagagatccg
    21361 gcgagttaaa cgtgacgatc tgcccggcgg tgatcagttg cgccacgccg cgcccggcga
    21421 ccatcaggat cagggtggcg acaaacggct gaattttgag gatcgctacc agtatgccgt
    21481 tccacaatcc cgctaggatg ccggtgccca gggcgcttaa caaaacaatc ggcaggctga
    21541 atcccgcgac cgtcatcgca gccgttgtgg ctccggcgat agccatcacc gcccccacag
    21601 agagatcgat accaccggtg gcgatcacca gcgtcatacc aatcgccagt aacgcaacgg
    21661 gagctgcacg gttaagaatg tcgatagggc taccgaacaa acgcccatcc tgaagcacca
    21721 cctgccagaa atgcggggct accaggctat cgaccagcag caccagcaac agtgccgcca
    21781 gctgcggcat ccccgtcggc cagtgaaaac gcctttttgg cggcgtagtg tcagggagag
    21841 attgaggcat cacactgttc tccttacgcc gcaatggcgt tcatgatcgc cggaacggaa
    21901 agctgtgcca gcgggatctc cgccacctgt ttgcgatcgc gcatgatgat cacccggtcg
    21961 gcatagccca ccagctcttc cagttcggag gagatcacca gcagcgccag accgtcagca
    22021 cacagcgttt caatcaggcg gatgatctcg gcgtgggcac caacatcaat gccacgggtt
    22081 ggctcatcaa ggatcagaaa ttgcggtcgg gtcagtagcc aacgtgaaag cagcactttt
    22141 tgctgattgc caccggagag aaattcaatc ggttgttcag ttgaaggtgt gcgaatgcca
    22201 agctggcgga taaagcgttc ggcaatctct tgctgctctt tgcgggaaat gggtcgtagc
    22261 cagccgcgct gggcctggag agcgaggatg atattttccc gcaccgaggc ggcggcgatg
    22321 atgccatcgg ttttcctgtc ttccgggcag aatccgatgc ccagtactga agcctgatgt
    22381 ggcgatcgca gggtttgcgg tttgcctttg atcaacgccg tgccgctgtc agcaggtttg
    22441 ataccgaaga tcacttcggc ggtttcggta cgtccggatc ccagcaagcc agccagaccg
    22501 acgatctcgc cggggcgcac ttcgagatca aacggtgcta tcgttccttt tttgccgtaa
    22561 tttttgaacg cggcaacggg tttgtcgctc aacaatgttc gcccggcacg ctgtagcacg
    22621 tgggtatcca gctcgcgccc cagcatcatt tttaccagtt cgatctgcgg tagctcgcgc
    22681 gtttcccgac agcctacgaa actgccgttg cgtaagacag tgatccgatc gctgacctga
    22741 tagacctgat cgagaaagtg agtgacaaag atcaggctga cgccgcgatc gcgcaactga
    22801 cgcatcaggc caaataatag ctccacttcc tgggtgtcga ggctggcggt gggttcatcg
    22861 aggatcagca ctttggcgga gagatcgata gcccggcaaa tggcgacgat ttgctgcatc
    22921 gcgactgaaa agcggttaag cggttcgcgc acgtcgagga agaaaccgta agatgccatc
    22981 agttcggtgg cgcgcttttc catctcttta cggcgtagaa ggccgaaacg tttgggttcg
    23041 cgacctatga atagattatc agcaaccgac atattgggta gcaggttgac ttcctgatag
    23101 acggtgccaa taccgagttg ttgtgcatgg gcggtatttt tcggtgagat agcctggcct
    23161 tccagccaga tggtgccgcg atcggcgtgg tatacaccag ttaatgcttt gattagcgtt
    23221 gattttcccg ccccgttttc accaagcagc gccatgattt cgccacggcg caggctgaaa
    23281 tcaacgttgt ctaacgcttt gacgccgggg aaaaatttac ttaatccttc ggtgcggagg
    23341 atctcctggt gttggtcggt cgtcatggtt tcccccggtg taaagcgtgc tcccagtagg
    23401 catgataaga cgcggtaagc gtcgcatcag gcttcagtgc accatcgccg gatgcggcgc
    23461 gagcgcctta tccggcctac gaatcggcat caggcatagc aaccctcaat accccatatt
    23521 tttcttcttc tctaactctt cttttgcggt atcaggcagg taaagggtgg atttggttaa
    23581 cgtcagcttt tcaggcatgg tgccgtcttt tttgtatttc tccagcgcat cgaaagcggg
    23641 tcccgccatg ttcggcgtca gttcaacact ggcgttcgct tcgccatcca tcatcgcttt
    23701 gtagatgtcc ggtacaccgt caatggaacc cgtgaggata tctttgcccg gtttcaggcc
    23761 cgcttcttta attgcctgaa ttgcaccaat caccatgtcg tcgttatggg cgtaaaccat
    23821 gcagatgttt ttgccgttgt tttccgcttt gataaagctc tccatgactt ctttgccttt
    23881 actgcgggtg aagtcacctg actgcgagcg gataattttg atatttggcg cattcttaat
    23941 ggcttcggca aagcctttct tacggtcaat ggcgacgctg gccccaacgg tgccctgcag
    24001 ctccaccacg ttgcatggtt tgccattcac ttcttttacc agccagtcac cgatcaactt
    24061 accttcgagg atgttgtcgg cagtaacggt ggtcatatag agagatttgt ctttcacatc
    24121 gatggaacgg tcgagcaaga agaccgggat ttcggcatct ttcgcctctt ttaataccgg
    24181 ttcccaaccc gtcgcaacca ctggagcgat aaagatcgca tccacccctt gtgcaacgaa
    24241 ggaacgtacc gctttaatct ggttttcctg cttttgctga ccatcggcaa ttttcaacgt
    24301 aattccgcgt ttttcggcct cacttttcgc cacattggtt tctgcggcgc gccagccgga
    24361 ttccgatccg acctgcgaaa atcctccggt taatggagct gccaacgcca tagacgacat
    24421 ggctgccgag actgcagaga ctacaagtaa gcgtttccac atagggttgt cctcgtcggg
    24481 gtaattatta ttggtgaaaa gatgttcgcg aaaaaactat agacaattcg ttatgtaacg
    24541 gattgcgtta catcacactt caaaaaagta aataaaacgt taatcacaag tttgtaatcg
    24601 ctttcatctc actatgaaaa atgcggctac ggttatggat tttcctgctc tgtataccgt
    24661 cttaaaactg gcgaaaaagg aaaatgaaga cgaaaacaag caaagacatt cggcgcgagt
    24721 tggctataat actcggcact tgtttgccac atatttttaa aggaaacaga catgagctta
    24781 ctcaacgtcc ctgcgggtaa agatctgccg gaagacatct acgttgttat tgagatcccg
    24841 gctaacgcag atccgatcaa atacgaaatc gacaaagaga gcggcgcact gttcgttgac
    24901 cgcttcatgt ccaccgcgat gttctatccg tgcaactacg gttacatcaa ccacaccctg
    24961 tctctggacg gtgacccggt tgacgtactg gtcccgactc cgtacccgct gcagcctggt
    25021 tctgtgatcc gttgccgtcc ggttggcgtt ctgaaaatga ccgacgaagc cggtgaagat
    25081 gcgaaactgg ttgcggttcc gcacagcaag ctgagcaaag aatacgatca cattaaagac
    25141 gtaaacgatc tgcctgaact gctgaaagcg caaatcgctc acttcttcga gcactacaaa
    25201 gacctcgaaa aaggcaagtg ggtgaaagtt gaaggttggg aaaacgcaga agccgctaaa
    25261 gctgaaatcg ttgcttcctt cgagcgcgca aagaataaat aagttcttct agcgcaataa
    25321 ccctgaacgc cgggcttcgg ttagtaaggg ttttttatgc ccgcgataaa taaactctct
    25381 attccaccat cattattctc agcggttgca aggcttgaac ggtaagaaca agcaaacccg
    25441 accaccattt tgctgttcat agccacttgc tggaagttag ccgacctcac tcatactcac
    25501 cgttcaaagc gtgcccccgt tttacccctg cacatttgtg aacgccagca gagtttttta
    25561 tgcgattgca gatatggaaa gcggctcgga gagaaaagca aaagatggaa aagttacaaa
    25621 aaaaaatcgc gagatacatc gcataaaaca acgccacccg aaggtggcgt gcttttatta
    25681 ttacttatcc ctgtctaacc agtcgccgct ttcaattagc tttaatccat cgacgggtcg
    25741 ttgataaacg tacatccatg cactcccgta cggcgtctga atcaactggc gcgcgtattc
    25801 accgcccctg gtgcgcaagg catcaagttc ggccagcgtg gcgttgtcaa tacgataaac
    25861 ttcaccgtgt accgttccgt tccccggaac tgcgcctgga tagtggccca ggctatacaa
    25921 ctggtagtta tcgatactga aatcgcccag taactgggca ttggtcatcc agtgactgtt
    25981 gccttgtttg tggcgtaaac tgccgtagac aaatattcgc attgctaaaa ctcgaactga
    26041 tagagcaaat ccagtgcctg gtctacacca gacacggctt ccagatatag cttaggcatc
    26101 aggcgataac gtaacgtgag tgttgctata gagtcaaata tacccacgcc gtatttcact
    26161 tgcagacctg gcaatacata gccgctgacc actacctggg aggagtcgcc tactccctgg
    26221 gtgtcgagcg ctaaattgct tacgccaaac gtctcgccga ttttacccac aatctggcca
    26281 ctttgcgcaa cccccagacc aatcagcatc gaggtcattg ccgcactgtc gctctgatcg
    26341 ctctccagcc cttgtccacg tagcaaataa gacaatgcag cttgttgcga catcgccggg
    26401 tcagagaaga tctccgcttt cggttcgtcc gccagaccag tgacgcgaac tccggcaatt
    26461 acgtcgtctt ctgtagcatc cgggttacga atagcttcaa tattaagata cggttgatcc
    26521 ggcggaccag agaacagtaa ctcaccctta cgcacaatca gatcctgacc ataggcatgg
    26581 aagcgccctt cagggatgtt gatctgcccg ttcaggccca gcccttgttt gtcctgtacg
    26641 acgttaagat cgcccgtcag ccgcgctttc aggccaaagg cgtcaatgcg cacattgttg
    26701 ccaacgtgga caatcaggtt actgttaatc ggaatcgacg ccgttttcgg ctcttccggt
    26761 tgcaggttat cgttaagcat caccacatcg ctggagacgc ctactgcgct ttccggcaga
    26821 tcgtgcacca cgatgcgcgc ccacgggaca tccacgcgac catcgagggt aaacaggttt
    26881 ggtgtagcct cgaatacaac atctggcgat acatccattc gtaccatcgg cggcacggtg
    26941 atccgcactt tactgccttt cgccgttact cgtgcccgcc agttttcaat ttggctccag
    27001 tcggcatcac cgttcaggta gatttcaccc tgctgggtcc gtactgtacc ggcaagcgtc
    27061 gagcgcatac cgttaaagtt gaccgcaagc tggctcggct gcatatcaaa cggcataaag
    27121 ttgccgtcga tatccacacc cgtaacctga agctgaccaa acaactgcgg gctttgcaca
    27181 tcaccaccca gacgcaagtt ggcactcacc atccccgctg ctttttcccc acgggtaaag
    27241 atggggttta tcatcgcaag gttgaagtta cggatattga cgttgccacc aagattacgg
    27301 cggccttgcg gatcggtcac ctgcacctgt ccatcaaact ggccgttatt ggtcaggcgg
    27361 atggtccagc ccaattcggc acggttgtta cgcaattccg ccgtcagatt cagtgtctga
    27421 aacgccaccg gcagcgccgc atcgttgacg gtttgcgtta cctgcacgtt acgccccgaa
    27481 agggtgatac tgccctgcgg cagcccctct ttcgtggtgt cccaggcaac atccgcttta
    27541 cccgtgaaga taccgctggc ctgagtggtt tctggcataa acggtttcag catggcgagg
    27601 tcgaagcggt tgagattcac caccgcacgc ccttcggccc ccgcatcgat agtttgcggc
    27661 acgcacagtt ccgcattcgg gttaagccag cagtgtggcc cgatgctgat tttttgctcc
    27721 ttattgcggt aatccagcgc aatatcgcgg gtcagcgacc acgggccgac cggcgtctgg
    27781 aaacgggtat tgctaagagt tcccttccag cgttcttctt tgcgatcaaa acttcctgcc
    27841 agattaagct gcccggagac aggctcgccc tgaatccgca actgtagctc gtgctgcttt
    27901 tcgctgcctt tggcattcag ggtgacgagg ttgatattta catccggctg cgaaatttgc
    27961 tcaacgcgta cgtcgagttt cccggcgatc tgatcggtgg atttaatgtc gccttccacg
    28021 cgaacctgcg ccacggaaag ttcctgccag cgcaggccgc gcgcggtgat atctgccagt
    28081 agttgtggcg cttccaccgt gccgcgtact ttcaccagcc ctttcgctgt accgccaagc
    28141 cccggcagcg cgttatccag ccccggcgcg ttgatggtgg catcaagatt gagatctttt
    28201 acccccagct cgcctttcac ttcggcactg tttggcccga gttccagatg aagcccaggg
    28261 atcatccact gcatataact gttgcctttc agcgtgccgt caacgttcac tttgttctgt
    28321 ttgacgttac cggtcagctt cagttctggc acctccatct gccaggtgcc gccgtacagg
    28381 ctaccgcggg ttttaatcaa gccattgagt ttcgacggcc actccgggaa ctctttggcg
    28441 gtgttaatgc cattaagcgt tagctcaccg cgccaactaa tggcctgctg ccagtcgagc
    28501 aacgccttga gttcagtttt cccttccagc gccgcgacgg tgagtttgtc gagattgacc
    28561 tgctgttcat tacctttggc atcaagggta atggtggctg gcgggatctc ctgtcccttc
    28621 actgccgtac gcatagagag cgtgtaatcg gtcattttgc cggtaagttt cagtttcaga
    28681 tcatccgcct gatactgctt ctcaccagtg aacggccagt aaagctgttt gctgttcact
    28741 tccacgttga gtggcaatcc ggcttccgcc agtcgcgtct gggcgcgtaa atccatatcc
    28801 accggaccgg aaaggttaac gcctatctcc agctgttcgc gcagcgcgcc gcccactttc
    28861 agcttcacct tttcaccttt caacggctcc acgttcagtg tgctgttgag gatgatatcc
    28921 accggccagt tgtctgacag ctgcgccgtg ccgctggcgt tgacgatccc ctggttggaa
    28981 tcgatatcca gggcgtccag tttagtattg ccgtcaatgc tgctcacttt cagcagcatg
    29041 gtgcgcacgg tgatgtccgt gtcgcccgtc acgcgcagct gctcgccctt aaactcttca
    29101 atgttcaggt taagcggcaa atgcacgtcg gtcatttccg gcaataccgg gcgagaaaag
    29161 agatctttca gcgtttcgcc gagcggcttt tcatccggct gcggattttc aatcttcggt
    29221 tcgacgacct cttcctgcgc cacttccgcc actttcggca gagcaatcag caggcctttc
    29281 agcgacgtcg gtttcagggt cagggttttc tcctgccagt tcaggccgga ggtgaagtcc
    29341 atcaccgaca ccgtggtgtc atcaatcttg atgttgacgt tatccagcgc cacccgtgtc
    29401 agggtgatgg gatacggcgt ggagagatcc agcggaccgc tatcttcctc ttcttcaacc
    29461 tgttcagaag gaggcatttt tttactgtcg atgttgacct gaatgtcttt cagcgccagg
    29521 tcattaatac aaacgctact gttccacagg cactcaagac cgaccgccag atgcagatta
    29581 cccgctttta ccgccacgcc tggctgctca taacgaacgt cagacaaggt gagatcgcgc
    29641 cagccgccgg tgacgttgcc aatatccagt cctggcaccc agcgatccgc cgctttaaat
    29701 acaagatgca ggccgctggt ggtgcccacc agaaacgcca ccgatcccag caacagtaag
    29761 ataacgataa ccacgccgag gctgattttt ttccataaac tcataattct ggccccagac
    29821 cgatgtaaaa ctgtaacccg tgttcgtctt tatccgcgac cggtacggca aaatcgagtt
    29881 tgattggccc gaccggcgat tcccagcgta cgccgccccc ggtaccggtt ttaaagtcgc
    29941 tgcggcgaat atcgcttacc gcttcgccac tatcgacaaa caccgcgccc caccattttc
    30001 cggtcacgtt gtactggtat tccagcgatc cggttatcag cttcgaggcc cctttcaggt
    30061 caccgttggc gtatttcggg gcgatagatt tatatttgta gccgcgaata ctacgatcgc
    30121 ctccggcgaa gaaacgcaga tccggcggta ctttgtcgaa atcaccggtt tcaatccagc
    30181 ccagcgtgcc gcgtgtcaca aaacgatggc gatcgtacag tgtgcggatc cagacgttct
    30241 gcgcctggaa aacggagaaa tcaacatctg aaccccaggc cgtgttggag tagtcgatag
    30301 agtagcgttg cgagtcgccc caggttggca tcaggccacc acgagaacgc gtgcggctaa
    30361 tcatcacccc aggataaaac agcatcgtgg tgttggtaat ttcaccctgg gtaaagtggt
    30421 cgaggctcca gcgcaggtta atggcacgct gccagtcgct ggagagatcc cagtagcgag
    30481 aagccaccag cgtagtggag tcagattcgg tatcgttcag gtcagtgcgc ttaaaaccgc
    30541 cctgcaccaa ataatattgt tccagtggat tcttcagcag cggcatttta tagctgaagt
    30601 cgaggatctg ttccggcgcg gaaatactgg tactggtggt cagactgtga ccataagagt
    30661 tcatccacgg ctttttccac gtcgctttca cgcgcggtcc cacgtccgta gagtaaccga
    30721 ccccggtttc gatggtgttt tctgttcgcg gcgaaaccac gcccgtcaat ggtaatactt
    30781 tcgtttcgcg cgctttatca aattgcggtg ccactaccac cgaattaaac cagccggtag
    30841 cagaaagtcg gcggttcagt tccgccagat ctttcgattc atactcatcg ccctctttaa
    30901 acggcaccag attttgcagg tattcatcgc ggatttgtga tccttcaaag gtcacatgcc
    30961 caaagcggta acgttcgcca ctgttataat caatatccca gaaggcttta tgcaggccga
    31021 gcgcaatgcc cagctgcgct ttggtaaatt cgctatcgaa ataaccttta cgcaacgcaa
    31081 tactggttaa ggactttttg aaattttcat aatcgccctg gttcagtacc gtaccaatag
    31141 ccgggcgagt atcgagcaat ttcaaatagt ctttatcggt ccgcgcgccg ccgcgcaata
    31201 ccacatcggt gccgccaatt aacaccggta cacctggcgt gactttggcg atcaatacct
    31261 gccgcccttt ctttggcggt ggacggagat caaattcaat ggtcggctgg taataaccca
    31321 gcgctttcag accttcacgg atggcatcat cgacgcgtgc gcgaaagcga cggtctggcg
    31381 tcacttcatc actttcaatc gtagaaagct gcgcacgaac gttcttttcc agctgtcccg
    31441 ataacccctc gacctgtaga cggacgttcg cggcgacggc agatccgctt aagcagagta
    31501 agcttacaca gcataactgt cggatatagc gcacattttc tcctgaatat ccttttttcc
    31561 tacccctgga acgccgttaa acggcttaac aaaaatccag taatatggat taaaaagcag
    31621 actaaacccc aaatatttct tatgttttac tttagaccta ttcacggtgg ttattgtgtg
    31681 caaatacgcc tcttgttaca accttaaccc caatgaccga ttttcgggag agcgacacca
    31741 tgagtttatt tgataaaaag catctggttt cccccgccga tgccctgcct ggacgtaaca
    31801 ccccgatgcc cgtagccacg ctgcatgcgg tcaacggtca ctcaatgacc aatgtacctg
    31861 acggaatgga gattgccatt tttgcgatgg gttgtttctg gggtgtggag cgtctgttct
    31921 ggcagttacc cggcgtttac agcaccgccg caggctatac cggcggctat acgccaaatc
    31981 cgacttatcg ggaagtgtgc tccggtgata cgggtcatgc cgaagcggta cgcattgttt
    32041 acgatccttc cgtcatcagc tatgagcagt tgctacaggt attttgggag aatcacgatc
    32101 ccgcccaggg aatgcgtcag ggcaatgacc acggcacgca gtatcgttca gcgatttatc
    32161 cgctgacccc tgaacaggat gccgcagctc gcgccagtct ggaacgtttt caggcggcga
    32221 tgcttgccgc cgatgacgat cgtcacatca ccacggaaat cgctaacgcc acaccgtttt
    32281 attatgccga agatgaccac cagcaatatc tgcataaaaa cccatatggt tactgtggaa
    32341 ttggcggaat tggcgtctgt ctgccgccgg aagcatagcg ttacgggtac aaatgtagtt
    32401 tgttgataaa gtgctctttg tctatgcctg atgcggctta aacgacttac caggcctaca
    32461 aaatcgtcca aattcaatat attgcaagga ctgcgtaggc ctgatcggca tagcgcatca
    32521 ggcagtattg catttatcag cagtctgaat tttaaccctc tggcgacttt acagtacctt
    32581 acgctatact agccactgaa aatgtcggtt cactttcttc gaatcggctt tcaatgtgta
    32641 tttcacacaa attaatcaac tttcccttcc gaggatctgg cctgaaaggt cggataagat
    32701 atgttaaaca gtattttagt catactctgc ttgatcgctg taagtgcgtt cttctcgatg
    32761 tccgagatct cgcttgccgc ctcacgcaaa atcaaactta aactgctggc tgatgaaggc
    32821 aatataaatg cccaacgcgt tctgaatatg caggaaaatc ccggcatgtt ctttaccgtg
    32881 gtccaaatcg gtctgaacgc agtggcgatt ctcggcggta tcgtcggtga tgcggcattt
    32941 tctccagctt ttcacagcct gttctcccgc tatatgtcgg cagagctctc tgagcaactg
    33001 agctttattc tctctttctc gttagtgact ggcatgttta tcctgtttgc ggatttaacc
    33061 ccgaaacgca tcggtatgat tgcgccagaa gcggtggctt tgcgtatcat caacccgatg
    33121 cgcttctgcc tgtacgtttg caccccactg gtgtggttct tcaacggcct ggcgaacatg
    33181 atcttccgta ttttcaaact gccaatggta cgtaaagatg acatcacttc tgatgacatc
    33241 tacgcggtag tggaagccgg tgcgctggcg ggcgtgttac gtaaacagga acacgagctg
    33301 attgaaaacg tctttgagct ggaatcccgt accgttccgt cttcaatgac accgcgtgaa
    33361 aacgtgattt ggtttgatct ccacgaagat gagcaaagcc tgaagaataa ggtggcggaa
    33421 catccgcact ctaagttcct cgtctgtaat gaagatattg accacatcat cggttatgtc
    33481 gattctaaag acctgctgaa ccgcgtgctg gctaaccaaa gcctggcact gaacagcggc
    33541 gtacaaattc gcaacacgct gattgtgccg gatacgttaa ccctttcaga ggcgttggaa
    33601 agttttaaaa ccgcaggtga agacttcgcg gtgatcatga acgagtacgc gctggtggtg
    33661 gggatcatca ccctcaacga cgtgatgacc acgctgatgg gcgatctggt cggtcagggg
    33721 ctggaagagc agattgtcgc ccgtgatgag aactcatggc tgattgacgg cggcactccg
    33781 attgacgacg tcatgcgcgt gctggatatt gacgaattcc cgcagtcggg caactacgaa
    33841 accatcggcg gctttatgat gtttatgctg cgtaagatcc cgaaacgcac cgattcggtg
    33901 aaattcgccg gctacaaatt tgaagtggtg gatatcgata actaccgcat cgaccagctg
    33961 ctggtgaccc ggatcgacag caaggccacc gccctttcgc caaaactgcc tgacgctaaa
    34021 gataaagaag aaagcgtcgc gtaacccccc agaaacatca acggctcctg aatcaggagc
    34081 cgtcttatta ctgcatagca ctttttactg catagcactt tggttaagcc atctctgttt
    34141 gcagacgcat aacctgacgg ttaacttcgg acatcactga caaatgcagc ttatccttca
    34201 cttttgggat aagaatctta cccttatcaa attcaaaagc gccaacgtcc ttgatgtata
    34261 accgtccacg gaacaggatc tttacgtact tcgccacctg aagtgggttg taccgttgga
    34321 aaattttcat tgttatctcc tgctgagtat tacgcctgtg cggtgccaca atcggcccaa
    34381 ctattatgag gcgcaaattt taatgcttag tgactataga ctatccgggc aatgtttcca
    34441 ccgcgtataa ctttttttac ctaaaggtta caattattca gaattatctt tttaccgaag
    34501 cgcggtcttc agtataagca ttcatttttc atatgatttg tgcgcttgac cgcaaactgg
    34561 catcacactt gcgggaaatt cgataaatag cacatatgat taaaactcag acccaagtgg
    34621 tcggatcacc tgcatatcat aagaaggaaa caccatgacc ctacgcaaga ttctggcact
    34681 cacctgcctg ctgttgccga tgatggcttc cgcacatcag ttcgaaaccg gtcagcgagt
    34741 gccgccgatt ggcattaccg atcggggcga gttggtgctt gataaagatc agtttagcta
    34801 caaaacctgg aacagcgcgc agttagtggg aaaagtgcga gtactgcaac atattgctgg
    34861 tcgcacctct gcaaaagaga aaaacgcgac gctgattgaa gcgattaaat cagcgaagtt
    34921 accgcacgat cgttaccaga ccaccactat tgttaacacc gacgacgcga ttccgggttc
    34981 agggatgttt gtgcgcagca gtctggagag taataaaaag ctttatccct ggtcgcagtt
    35041 tattgtcgat agcaatggcg tcgcgcgcgg tgcctggcag ctggatgaag aaagttccgc
    35101 tgtagtggtg ctggataagg atggtcgcgt gcaatgggcc aaagacgggg cgctcactca
    35161 ggaagaggtg cagcaagtga tggacctgct gcaaaagttg ctcaaataat ggcaacaaca
    35221 tagattctgc aaaaataatt ttcccctatc gttatctatg ggatagggga attaagtctc
    35281 tgagttctta cgccgaactc atactggtaa cgctagttgc tcttttttaa acgcctcaat
    35341 ggtcgactta tatttcttgc aaatcttttc tttggtaata ttactatctg cggccagatt
    35401 cgcacttact atcgcatcca cttgttcggc taattccact tgttggcaac ctgcattaac
    35461 cagaaaatta taaataaggg agtaatcttc atcctccaaa acaacagagg aaccattacg
    35521 aaaacgttca tcccctccat agattttaag cgcaaagccc aaagattcta tgactatggc
    35581 aaaattatca tcaattattt tttcgtgaat ataccatcca tcgcattctt taattccacc
    35641 tcctggtatt tggagttcaa atttaccccg ttttaatttg cttactatat cctccaataa
    35701 ggcataacct aaagacacgt caccaatcgc ctggtgaata taacctttaa tccttttcgg
    35761 tatgtcagac tgttttttct gcagaatatc acaggcatac aatcttctgt tttgttctaa
    35821 cggaaactcg cggtttttga aatccatgaa tgagttatac tcatttattg ttatgggaat
    35881 ttcttcgtat tcaaatccac tacctcgcaa tgtcattgta tattcggcac cattatcttt
    35941 aaggctgaaa ctgataagga acttatccat ggcgttaaca gttccatttt ttgatgaaag
    36001 tacctgataa ctatttttat caatctgagt tgcgcaacga aagaactccc tcaaagttaa
    36061 caggctatca taattcgaaa tccaatcttt tttcttaaat gcatgctgga tattgttaaa
    36121 aaaattgttt gaatgtgttt ttgtatacga tgactttaca aataactcca ccgcaccaat
    36181 aaaattattg tgtctgatcc tgggtaacat atgacaacct tattttattt ggttatttta
    36241 tgtcgccatt aaatatctca ttagagaagt aaactatcaa aaaacgctca ttattaaaat
    36301 gtaacttatt agcaattgaa tttttgcact ttaaatacaa tctcttttta tttataactt
    36361 tttaggttgg gagaaatcag gatggcagaa tcaggaaata cactgtttct gccatctgaa
    36421 tttagtaaat agacactctg aaccccggat tcaggaacga ctcgcgcgga gtgtaatcca
    36481 gcggtttacc ctgccagtcg tgaacgtgcg ctccggcagc tgcagccaca gcatgtccag
    36541 cggcggtgtc ccaaatattc gttggtccga agcgcgggta cagctgcgcc tgtccttccg
    36601 ccaccaggca gaatttcagc gaagagccga tggacgtggt ctgatgttcg ccaagctgtt
    36661 gcagatactc tttcagctcc gcatctgcat gggaacggct gataaccacc agcggcgggc
    36721 gcgcatcgcg gacctgaatc tgcttgcgca caccgcactc ttctttccag gccttgcctt
    36781 ctgccgcgct gtacattacg ttcattaccg gcgcatacac cacacctaaa atcggtttgc
    36841 catggtcaat gagcgcaatg ttaacggtga attcgccatt acgtttaata aactctttag
    36901 taccatccag cgggtctacc agccagtaac gctgccagtg ctgacggact tcccaaccgg
    36961 gaggatcttc ttcagaaagg accggaatat ccggtgtcag cgtacgtaaa ccgtccatga
    37021 taacggtgtg tgcggcaata tccgctgccg ttaccggaga attgtccgct ttgctgacga
    37081 cgtccatcgg tttcgtcccg tcgtagacct gcataatggc atcgcctgca ttccgtgcaa
    37141 gctggcatac ttgatctaac atttctccac ctcgtctctg tgagcggtgt taacttattg
    37201 ttttacttat accctatcgt taatgaatgc gccaactgtg atagtgtcat cattttcaaa
    37261 gcgtaaaatt gtggcattct tcactgttct ataagtaaga cgtttattct cccttttctt
    37321 tcgtattccc gatgataaaa ggatgtccct gatgattaag tttagcgcaa cgctcctggc
    37381 cacgctgatt gccgccagtg tgaatgcagc gacggtcgat ctgcgtatca tggaaaccac
    37441 tgatctgcat agcaacatga tggatttcga ttattacaaa gacaccgcca cggaaaaatt
    37501 cggactggta cgtacggcaa gcctgattaa cgatgcccgc aatgaagtga aaaacagcgt
    37561 actggtcgat aacggcgatt tgattcaggg gagtccgctg gcagattaca tgtcggcgaa
    37621 aggattaaaa acaggtgatg ttcatccggt ttataaggcg ctgaatacgc tggattatac
    37681 ggtcggtaca ctcggcaatc atgaatttaa ctacggtctg gattacctga aaaatgcgct
    37741 ggcgggagcg aaattccctt atgtaaatgc caacgtcatt gacgccagaa ccaaacagcc
    37801 aatgtttaca ccgtatttaa ttaaagatac cgaagtggtc gataaagacg gaaaaaaaca
    37861 gacgctgaag attggctata ttggcgtcgt gccgccgcaa atcatgggct gggataaagc
    37921 taatttatcc gggaaagtga cggtgaatga tattaccgaa accgtgcgca aatacgtgcc
    37981 tgaaatgcgc gagaaaggtg ccgatgtcgt tgtcgttctg gcacattccg ggctgtctgc
    38041 cgatccgtat aaagtgatgg cggaaaactc agtttattac ctcagtgaaa ttccgggcgt
    38101 taacgccatt atgtttggtc atgctcacgc cgttttcccg ggtaaagatt ttgctgatat
    38161 cgaaggggct gatatcacca aaggcacgct gaatggtgtt ccggcggtaa tgccgggcat
    38221 gtggggcgat catcttgggg tggtcgactt acaactcagt aatgacagcg gtaaatggca
    38281 ggtgacgcag gcgaaagcgg aagctcggcc gatttacgac atcgccaata aaaaatccct
    38341 cgcggcggaa gacagcaagc tggtagaaac actcaaagcc gatcacgatg ccacacgcca
    38401 gttcgtcagc aagccaatcg gtaaatctgc cgacaatatg tatagctatc tggcgctggt
    38461 gcaggacgat ccgaccgtgc aagtcgtgaa caacgcgcaa aaagcgtatg tcgaacatta
    38521 cattcagggc gatccggatc tggcaaaact gccggtgctt tcagctgccg caccgtttaa
    38581 agttggtggt cgcaaaaatg acccggcaag ctatgtggag gtggaaaaag gccagttgac
    38641 cttccgtaat gccgccgatc tttatctcta ccccaatacg ctgattgtag tgaaagccag
    38701 cggtaaagag gtgaaagagt ggctggagtg ctccgccgga cagtttaacc agattgatcc
    38761 taacagcacg aaaccacagt cactcatcaa ctgggatggt ttccgcactt ataactttga
    38821 tgttattgat ggtgtgaatt atcagattga tgttacccag cccgcccgtt atgacggcga
    38881 gtgccagatg attaatacca atgcggaaag gattaagaac ctgaccttta atggcaagcc
    38941 gattgatccg aacgccatgt tcctcgttgc caccaataac tatcgcgctt acggcggcaa
    39001 atttgccggt acgggcgaca gccatatcgc ttttgcttca ccggatgaga accgctcggt
    39061 gctggcagcg tggattgctg atgagtcgaa acgtgcgggg gaaattcacc cggcggcaga
    39121 taacaactgg cgtttagcac cgatagctgc cgataagaaa ctggatatcc gtttcgaaac
    39181 ttccccgtca gataaagccg cagcgtttat taaagagaaa gggcagtatc cgatgaataa
    39241 agtcgcgacc gatgatatcg ggtttgcgat ttatcaggtg gatttgagta agtaaaacac
    39301 ttctttttcg gcctataaat catcaatcgc atccggcatt tattggcgga tgcgatgctg
    39361 gcgcatctta tccgccctac aagtcataca ccgtaggccg cgcatccggc agttatgccg
    39421 cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa ctcaatccag tccgccagtg
    39481 cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag gctgtattca acatgcggtg
    39541 gcaccaccgg atacgcgata cggttaagaa aaccatcctg ttctaacgcc tgtaacgact
    39601 gcgcaagcat cttttcgctc accccgccga ttttgcgccg caggtcgcta aagcgatgag
    39661 taccttcgcg tagcgccacc agaatcaaca ccccccaacg gctggtgacg tgtttcaaca
    39721 cctcgcgcga cgggcactgt tccgcaaaga gattaccctc tttcagttgt tgcgacaggc
    39781 taacctgact catttcatac ttaccttttt gtacgtactt actaaaagta agtttaggtg
    39841 ttagcgtatt taaacacaag acaaaacgat ggagacttcc catgatcgct attactggtg
    39901 ccactggcca acttggtcac tatattattg aatccttgat gaaaacggtt cctgccagcc
    39961 aaatagtggc tatcgttcgt aatccggcaa aagcccaggc tctagcagca caaggcatta
    40021 ccgtgcgtca ggctgactac ggcgatgaag ccgcactgac atctgcactt cagggagtgg
    40081 aaaaactact gctgatctct tccagcgaag tgggtcaacg tgccccgcag catcgtaatg
    40141 ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc ttataccagc ctgctacatg
    40201 cagatacctc cccgctcggc ctcgccgatg agcacattga gacggagaaa atgctggctg
    40261 attctggcat cgtttacacc ctgctgcgca acggctggta cagcgaaaac tacctcgcca
    40321 gcgccccggc agcactggaa cacggcgtat ttatcggtgc ggcgggcgat ggcaaaatcg
    40381 cctcagcaac gcgggcagat tatgcggcag ctgcggcacg cgtgattagc gaagccggtc
    40441 acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg gacgttgaca cagttagcgg
    40501 cagagctgac caaacagagc ggcaaacagg ttacctatca aaatctgagc gaagccgatt
    40561 tcgccgcggc actgaaaagc gtcggactgc ccgacggact ggcggatatg ctggcggatt
    40621 ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag caaaacgctt agcaaattga
    40681 ttggccgccc aacgacaacg ttagccgaaa gcgtaagcca tctttttaat gttaataact
    40741 agttaattaa agtggcatcc tcccgcatcc tctctgataa tgacgggatg ccgggagcaa
    40801 tcatgtctgc ttcctgaact ttcttctgac agaccaatgg atgccagtaa tgattagcgg
    40861 cgtgctgtac gccctgttag cagggttgat gtgggggctt atttttgtcg ggccgttgat
    40921 cgtaccggaa tacccggcga tgttgcagtc gatggggcgt tatctggcgt tagggttaat
    40981 tgcgctgccc attgcctggc tgggacgcgt gcgtctgcgt cagttggcgc gtcgcgactg
    41041 gcttaccgcc ttgatgctca ccatgatggg caaccttatc tattacttct gccttgccag
    41101 tgccattcaa cgtactggcg cgcctgtttc cacgatgatt atcggcaccc tgccggtggt
    41161 gattcccgtt tttgccaatc tgctttatag ccagcgcgac ggcaaactcg catggggaaa
    41221 actcgccccg gcactggttt gtattggcat cggcctggcg tgtgtgaata ttgctgagtt
    41281 aaaccacgga ctccccgatt ttgactgggc acgttatacc tctggcatcg tgctggcgtt
    41341 agtttccgtg gtctgctggg cgtggtatgc cctgcgcaac gcccgctggc tgcgggaaaa
    41401 tcccgacaaa catccgatga tgtgggcaac ggcgcaggcg ctggtcacac tgccggtttc
    41461 tctcatcggc tatctcgtcg cctgttactg gctgaatata caaacgccgg acttctcctt
    41521 accttttggc ccccgtccgc tggtgtttat tagtctgatg gttgcgatag ccgtgctttg
    41581 ctcatgggtt ggcgcactct gctggaacgt cgccagccag cgattaccga cagtgattct
    41641 cgggccgctg attgttttcg aaacgctggc aggtttgctg tacacctttt tactccgcca
    41701 gcaaatgccg ccgctaatga cgctgagcgg tatcgcgctg ttagtgattg gcgtggtcat
    41761 tgcggtcaga gcaaaaccag aaaaaccttt aactgaatct gtctcagaaa gttgacacgc
    41821 tggcagtgag ttaaataagc ctctgctacg taagggttat agcttttgcc ttaaagatgc
    41881 atttaaaata catcttatat tattaagaat gaggtatcag ctatggctta tcgcgaccaa
    41941 cctttaggtg aactggcgct ctctattcct cgcgcttcag ctctgtttcg taaatatgat
    42001 atggattact gctgtggcgg taagcaaacg ctggcgcgcg cggcggcacg taaagaactg
    42061 gatgttgagg tcattgaagc tgaactggca aagcttgctg aacaaccgat tgagaaagac
    42121 tggcgtagcg ccccgctggc agaaatcatt gaccatatca tcgtgcgcta ccacgatcgt
    42181 caccgcgagc aactgccgga gctgatcctg caagcgacta aagtcgagcg cgttcacgcc
    42241 gacaaaccga gcgtgccaaa agggctgaca aaatacctga ctatgctgca tgaagagcta
    42301 tccagccaca tgatgaaaga agagcagatc ctcttcccga tgatcaaaca aggcatgggc
    42361 agccaggcaa tgggaccaat cagcgtgatg gaaagcgagc acgatgaagc gggcgaactg
    42421 ctggaagtga ttaaacacac caccaataac gtcacaccgc cgccggaagc ctgcaccacc
    42481 tggaaagcga tgtataacgg tattaatgaa ctgattgatg acctgatgga tcacatcagc
    42541 ctggaaaaca atgtactgtt cccacgcgcg ctggcgggtg agtaatctaa aagctggatg
    42601 gcattgcgcc atccagcatg ataatgcggt tatttccgca gttcagcagc ccgcttctta
    42661 ccaataaaca accagcccag ccccagtgcg ataaaccaca gcggggtgac caacagcgcc
    42721 tggcgagtgt catcttccag tgtcagcaac acgaccacga acacaaagaa cgccatacat
    42781 acccagcaca tcagcttgcc gagcggcatc ttgtagatcg acttctcatg cagatgagga
    42841 cgctgtttgc ggtaaaccag gtacgagcaa aggataattg tccagacgaa cataaacaga
    42901 atcgcggaaa cggttgtaat catcgtgaac gcgccaatca cgctaggatt cacatacaac
    42961 atcaccacgc cgccgagcag acagatacac gagaaggtca accctttcgc gggtactgcg
    43021 cgcttagaaa gtttagcgaa cgctttcggt gccacacctt cctgcgccag accaaacagc
    43081 atacggctgg tagagaagac gccgctgtta gcggaagacg ccgcagaggt cagcaccaca
    43141 aagttgatca cgctggcagc cgcaggcaaa cctaccaaca caaacagttc aacaaacggg
    43201 ctcttctccg ggactaccga actccacggc gtcacggaca taatcacaat cagcgcgaag
    43261 acgtagaaca taatgatacg gatcggaatg gagttaatcg cgcgtggcag tgatttctcc
    43321 ggatctttgg tttccgcagc ggttgtacct accagctcaa tccctacgaa agcgaaaacc
    43381 gctatctgga atccggcaaa gaagccactt aaacctttcg ggaaccagcc gccatcattc
    43441 cacaaatgcg cgaatgacgc ttccacacca gtcggtgact gaaagtgcat cgccaccata
    43501 accaggccga cgacaatcag cgacacaatg gcgacgattt tgatcatcgc aaaccagaac
    43561 tccatctcac cgaacatttt cacggtggcg agattgagcg tcagcagcag cactatcacc
    43621 gccagcgagg cgacccagtc ggagagatcg gggaaccaga actgagcata agcagtgatc
    43681 gccaccacgt ctgccatacc ggttacaacc cagcagaacc agtaagtcca gccggtgaaa
    43741 tatcctgccc acggcccgag taaatcggaa gcgaagtcac taaaagattt gtattccaga
    43801 ttcgaaagca gcaattcccc cattgcccgc atcacgaaaa agagcataaa accgatgatc
    43861 atataaacga aaatgatcga cggcccggca aggctaatcg ttttgccgga ccccataaac
    43921 aaccccgtac caatggcacc gccaatggca ataagctgaa tatgtcggtt tgtgagattg
    43981 cgccgtagcg actgttcagc cggagcctga tcatcggcaa cgacttttac ctgatctacc
    44041 atgttttttt cttcctgtac ctgtctgtgt tgttcaggct ctacggcctt tagtcagtct
    44101 atgatacgac gaatctgtat cgggactcat cgatattagg taacaatacg cgggatgaat
    44161 actaagattt agatctaatg ttaattttat gtttaaagtg agtgtcatat cacggtgata
    44221 acgcgaaaca gctcacaaaa atacacgctt acggggtctt tgcaacacaa aatagcgccg
    44281 tttggcaaac tgaagggttt attgctgaat gcctgctccc ctctggttcg aggggaacag
    44341 aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa caccagggtg ctgaacggag
    44401 ggatggatgc gcctgcgccg cgctcgccat atgccagttc ctgcgggata gtcagttccc
    44461 atttagaacc taccggcatc agagtcagtg cttcaatcca gccagggatc acgccattaa
    44521 ccgggaattc agcgggttca ccacgagcaa cggagctgtc aaacacggtg ccgtcgatca
    44581 gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc cggaattgcg ccttcaccct
    44641 ggttgatcac gcggaattgc aggccagatt cggtgctatt cacaccttct tttttggcgt
    44701 tttcttccag gtatttcaca ccttcagcag ccatcgcctg gaaacgctga cgacgaacgg
    44761 catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac cacatcaacc ggcacagccg
    44821 gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag tgcttctggc agcagccctt
    44881 ccaggccaga ttcactcagt tgttgcccta cctgcaaacc aatgccgtag cttgcttgcg
    44941 cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc tttcttaggg tataaaagta
    45001 gcgggcagca tatcagccac acttctttgg gtaaaacttt gtcgcaggaa tgatgacaaa
    45061 tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat ataataaagg cgggtttacc
    45121 acggacatca tagcgttagc ttattcatag gctatgattg aggaacaaga cgcggagcag
    45181 gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc tggagaaagt ctggcacgcg
    45241 ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc atcgtcgggg gatcattatt
    45301 gccgccattg tattggtggt cggatttctg cttccatctg atgatacgcc caacgcgccg
    45361 gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat cacaaccgcc gacagaagaa
    45421 cagctgcggg cgcaattagt cacaccacaa aatgatcctg atcaggtggc tccggtcgct
    45481 ccagaaccta tacaggaagg ccagccagaa gaacagccgc aaactaccca aacgcaacca
    45541 ttccagccag atagcggaat tgataatcaa tggcgctctt accgtgtcga acccggtaaa
    45601 acgatggcgc agctgttccg cgaccacgga ctgcccgcta ccgatgtcta tgcaatggcg
    45661 caggtagaag gcgcgggtaa gccgctgagt aatttgcaaa atgggcagat ggtgaaaatc
    45721 cgccagaacg ccagcggcgt ggtgacgggc ttaaccattg atacggggaa taatcaacag
    45781 gtcctgttta cccgccagcc ggatggcagt tttattcgtg cgcggtagat gaagggtcaa
    45841 ccaaaagcca gctaaggatt gtctctttga tgaccttacg tctggcttcc agtgcctggg
    45901 gtgaaaccat actgatgtga taaaccaggc caagcgtatg ctggttgatc aaataatatc
    45961 ccccaagcgc agcaatatta atattcacct gcaatgggtc gatatctggc ttaaaaatat
    46021 tgtgtttctt cccttcatcc aataaggatt ccattaactg caagtgcgca tggttaattt
    46081 ctaagagacg cagtgatttt gcataatgca cacctttact ttgattttca ctatgcacaa
    46141 tttttaaaaa ccaagggtta
//
LOCUS       DAJTYZ010000014        45914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000014 DAJTYZ010000000
VERSION     DAJTYZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45914
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.014"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..717)
                     /locus_tag="OO596_000805"
     CDS             complement(<1..717)
                     /locus_tag="OO596_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6716749.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(731..1390)
                     /gene="pgmB"
                     /locus_tag="OO596_000806"
     CDS             complement(731..1390)
                     /gene="pgmB"
                     /locus_tag="OO596_000806"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6716750.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1387..3654)
                     /gene="ycjT"
                     /locus_tag="OO596_000807"
     CDS             complement(1387..3654)
                     /gene="ycjT"
                     /locus_tag="OO596_000807"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6716751.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGGSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3651..4706)
                     /locus_tag="OO596_000808"
     CDS             complement(3651..4706)
                     /locus_tag="OO596_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6716752.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4716..5504)
                     /locus_tag="OO596_000809"
     CDS             complement(4716..5504)
                     /locus_tag="OO596_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6716753.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5522..6574)
                     /locus_tag="OO596_000810"
     CDS             complement(5522..6574)
                     /locus_tag="OO596_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6716754.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6605..7208)
                     /locus_tag="OO596_000811"
                     /pseudo
     CDS             complement(6605..7208)
                     /locus_tag="OO596_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7195..8076)
                     /locus_tag="OO596_000812"
     CDS             complement(7195..8076)
                     /locus_tag="OO596_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6716755.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8097..9407)
                     /locus_tag="OO596_000813"
     CDS             complement(8097..9407)
                     /locus_tag="OO596_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6716756.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9442..10952)
                     /locus_tag="OO596_000814"
                     /pseudo
     CDS             complement(9442..10952)
                     /locus_tag="OO596_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11164..11478)
                     /gene="pspE"
                     /locus_tag="OO596_000815"
     CDS             complement(11164..11478)
                     /gene="pspE"
                     /locus_tag="OO596_000815"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6716757.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11553..11774)
                     /gene="pspD"
                     /locus_tag="OO596_000816"
     CDS             complement(11553..11774)
                     /gene="pspD"
                     /locus_tag="OO596_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6716758.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11783..12142)
                     /gene="pspC"
                     /locus_tag="OO596_000817"
     CDS             complement(11783..12142)
                     /gene="pspC"
                     /locus_tag="OO596_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6716759.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12142..12366)
                     /gene="pspB"
                     /locus_tag="OO596_000818"
     CDS             complement(12142..12366)
                     /gene="pspB"
                     /locus_tag="OO596_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6716760.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12420..13088)
                     /gene="pspA"
                     /locus_tag="OO596_000819"
     CDS             complement(12420..13088)
                     /gene="pspA"
                     /locus_tag="OO596_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6716761.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13255..14232
                     /gene="pspF"
                     /locus_tag="OO596_000820"
     CDS             13255..14232
                     /gene="pspF"
                     /locus_tag="OO596_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6716762.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14350..15615)
                     /gene="puuE"
                     /locus_tag="OO596_000821"
                     /pseudo
     CDS             complement(14350..15615)
                     /gene="puuE"
                     /locus_tag="OO596_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15653..16933)
                     /gene="puuB"
                     /locus_tag="OO596_000822"
     CDS             complement(15653..16933)
                     /gene="puuB"
                     /locus_tag="OO596_000822"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6716763.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16935..18425)
                     /gene="puuC"
                     /locus_tag="OO596_000823"
                     /pseudo
     CDS             complement(16935..18425)
                     /gene="puuC"
                     /locus_tag="OO596_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18575..19132)
                     /gene="puuR"
                     /locus_tag="OO596_000824"
     CDS             complement(18575..19132)
                     /gene="puuR"
                     /locus_tag="OO596_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6716764.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19159..19923)
                     /gene="puuD"
                     /locus_tag="OO596_000825"
     CDS             complement(19159..19923)
                     /gene="puuD"
                     /locus_tag="OO596_000825"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6716765.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20135..21496
                     /gene="puuA"
                     /locus_tag="OO596_000826"
     CDS             20135..21496
                     /gene="puuA"
                     /locus_tag="OO596_000826"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6716766.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21645..21809
                     /locus_tag="OO596_000827"
     CDS             21645..21809
                     /locus_tag="OO596_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716767.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21799..23184
                     /gene="puuP"
                     /locus_tag="OO596_000828"
     CDS             21799..23184
                     /gene="puuP"
                     /locus_tag="OO596_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6716768.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23318..23563
                     /locus_tag="OO596_000829"
     CDS             23318..23563
                     /locus_tag="OO596_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6716769.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23876..25519
                     /gene="sapA"
                     /locus_tag="OO596_000830"
     CDS             23876..25519
                     /gene="sapA"
                     /locus_tag="OO596_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6716770.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25516..26481
                     /gene="sapB"
                     /locus_tag="OO596_000831"
     CDS             25516..26481
                     /gene="sapB"
                     /locus_tag="OO596_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6716771.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26468..27358
                     /gene="sapC"
                     /locus_tag="OO596_000832"
     CDS             26468..27358
                     /gene="sapC"
                     /locus_tag="OO596_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6716772.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27358..28350
                     /gene="sapD"
                     /locus_tag="OO596_000833"
     CDS             27358..28350
                     /gene="sapD"
                     /locus_tag="OO596_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6716773.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28352..29158
                     /gene="sapF"
                     /locus_tag="OO596_000834"
     CDS             28352..29158
                     /gene="sapF"
                     /locus_tag="OO596_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6716774.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29226..29579
                     /locus_tag="OO596_000835"
     CDS             29226..29579
                     /locus_tag="OO596_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6716775.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29947..30735
                     /gene="fabI"
                     /locus_tag="OO596_000836"
     CDS             29947..30735
                     /gene="fabI"
                     /locus_tag="OO596_000836"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6716776.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30880..32006
                     /locus_tag="OO596_000837"
                     /pseudo
     CDS             30880..32006
                     /locus_tag="OO596_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32074..34008
                     /gene="rnb"
                     /locus_tag="OO596_000838"
     CDS             32074..34008
                     /gene="rnb"
                     /locus_tag="OO596_000838"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6716777.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34086..34189)
                     /locus_tag="OO596_000839"
                     /pseudo
     CDS             complement(34086..34189)
                     /locus_tag="OO596_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34243..36233
                     /gene="pdeR"
                     /locus_tag="OO596_000840"
                     /pseudo
     CDS             34243..36233
                     /gene="pdeR"
                     /locus_tag="OO596_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36380..36553
                     /locus_tag="OO596_000841"
     CDS             36380..36553
                     /locus_tag="OO596_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716778.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36643..37392
                     /locus_tag="OO596_000842"
     CDS             36643..37392
                     /locus_tag="OO596_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6716779.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37661..37879
                     /gene="osmB"
                     /locus_tag="OO596_000843"
     CDS             37661..37879
                     /gene="osmB"
                     /locus_tag="OO596_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6716780.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38005..38331)
                     /gene="yciH"
                     /locus_tag="OO596_000844"
     CDS             complement(38005..38331)
                     /gene="yciH"
                     /locus_tag="OO596_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6716781.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38331..39068)
                     /gene="pyrF"
                     /locus_tag="OO596_000845"
     CDS             complement(38331..39068)
                     /gene="pyrF"
                     /locus_tag="OO596_000845"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6716782.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39261..40430)
                     /gene="lapB"
                     /locus_tag="OO596_000846"
     CDS             complement(39261..40430)
                     /gene="lapB"
                     /locus_tag="OO596_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6716783.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40437..40745)
                     /gene="lapA"
                     /locus_tag="OO596_000847"
     CDS             complement(40437..40745)
                     /gene="lapA"
                     /locus_tag="OO596_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6716784.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40894..41658)
                     /gene="pgpB"
                     /locus_tag="OO596_000848"
     CDS             complement(40894..41658)
                     /gene="pgpB"
                     /locus_tag="OO596_000848"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6716785.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41828..42418
                     /gene="ribA"
                     /locus_tag="OO596_000849"
     CDS             41828..42418
                     /gene="ribA"
                     /locus_tag="OO596_000849"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6716786.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42482..45157)
                     /gene="acnA"
                     /locus_tag="OO596_000850"
     CDS             complement(42482..45157)
                     /gene="acnA"
                     /locus_tag="OO596_000850"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6716787.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45321..45416)
                     /locus_tag="OO596_000851"
     CDS             complement(45321..45416)
                     /locus_tag="OO596_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716788.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45530..45697)
                     /locus_tag="OO596_000852"
     CDS             complement(45530..45697)
                     /locus_tag="OO596_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716789.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45700..45864)
                     /locus_tag="OO596_000853"
     CDS             complement(45700..45864)
                     /locus_tag="OO596_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6716790.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10842 a        12141 c        11560 g        11371 t
ORIGIN      
        1 cgccgatgat ccaataaatc cggcaacaaa catattcgct ggttggttat aaaccgtttt
       61 cggcgcacca acttgctgaa caatcccatc cttcataatc acaatccgcg tcgccatggt
      121 catcgcttcg gtctgatcgt gggtcacgta gatcatggtg gtattcagct tctggtgcag
      181 cttgctgatc tctgcgcgca tttgcacgcg cagcttggca tcaaggttag agagcggttc
      241 atccattaaa aacacgcccg cttcgcgcac aatcgcccgc ccgagcgcca cacgctggcg
      301 ttgcccgccg gaaagcgcgc ccggcttgcg tttcaggtac tcacgcaggc cgagaatttg
      361 cgccgcccag ttcacccgct catcaatcac ctctttggcg attttttgca tcttcaggcc
      421 aaacgccatg ttgtcgtaaa ccgtcatatg cggatacaac gcgtagttct ggaacaccat
      481 cgctatattg cgtgctttgg ctggaacgtc attcattcgt ttgccgtcga tcaacagatc
      541 gccgccgctg atctcctcaa gcccggcaat catgcgcagg gtggtcgact taccgcagcc
      601 tgacggaccg acaaacacga tgaactcttt atcggcaatt tccaggttga agtccttcac
      661 cacatgcacc tggttatcgt agattttttg aatatgttgt aacgaaagct gagccatgtt
      721 gattcctttg ctatacgttt tgccagaagg ccgataaccg cgaccaggtc agggagtccg
      781 ttgaaggcaa tagtaattgc gccccggtta tgcccgcgcc gatccccacc gagcgcatac
      841 cgctggcgtt aatggcgtca atgcccgcct gcgcatcttc aatgccgata catgcctgcg
      901 gcggcacgcc cagcccggca caggcagcga gaaagatttc cggatccggt ttcgagtttt
      961 taagttggga agcatccgcg cagaaggtga aaaactcacg cagctccagt gcatctaaaa
     1021 tcgtcggcgc attgagggag acagaagcca gccctaccga gatctgttgt gcgcggagat
     1081 ctgccagcaa agagcgaatg ccgggtagaa cagcgttgac cgtcaactcg cgcaatgagt
     1141 ggacatagag cagatttttg cgatacgcca gttgcgccct ctcctgcgag ttaaagtcgc
     1201 cctctttgcc cccgtgttgc agaatgcgcc gcagagactc atcgcggctg atccctttta
     1261 gggattcgtt aaactgcgca tcaatgctga tgccaatttc agcggcaatc tgctgccacg
     1321 cctggaaatg cagatgcgcg gtatcggtga ttacaccatc cagatcgaaa attacctctt
     1381 gcagtttcat tcatcctcct gatgtgtggc agcggtccca ttgaagggca aaataaaatc
     1441 acccaaacag aaaacagatt cttcagccac ggatataagc tgaccgttca aacgcagtga
     1501 aacgggggca gaagttcgaa tcgcaatacg ctgcgcgtca agagtgacct gtaattcgca
     1561 gccctgccag aacagaggga aagacaactg ttgccactgc tcaggtaacg ccggattgag
     1621 atgcaattca ccgtcacgca cgctcccccc ggcaaaaccc tgaatcgccc ccagccagat
     1681 agcgccagtt gcggcagcat ggataccatc atcacaacta tgcggatcag caccaagatc
     1741 gatttcagtc ccctcgcgcc agaactgata actttgggtc agcaggccac agcgtgcggc
     1801 aacaatgccg tggattgctt tacttaatga cgagtcgtga atagtgcgcg gttcataaaa
     1861 ttgcagattg gcaagacacg atgccgctga gaactgctcc ggcagcatgt aattgagcat
     1921 caccacatca gcttgtttga ggatttgcat ctcgttcact tctgcgcgtg aataatccag
     1981 cagaatggtt tgcttccccg ccgccgcttt gtatttcgca agattaatcg ccggcttagc
     2041 cataaacgaa tcatcctgcg gcaaaacgcc gtcgggctga atttctggca gccgcagttc
     2101 tttaaggaac atttcggcac gatgaataaa cgcgtcgtcg ctacagccaa actggcgggc
     2161 aatactcagc gcctgctgga cgttgtaata cgccatatag ctggtgaagg cgttattatt
     2221 gacatgttcg gtatattcgt caggcccaat aacatcatga atttccagac ggtcgttaac
     2281 cctcaccgcg cggctaatcc agaactttgc agtttccaga agtagcgcca tgccttcatg
     2341 cgcaatgaaa ctttcatccc ccgtggtctg ccagtattga ataaccgccc aggcgatatc
     2401 ggccaccaga tgatgttccg cctgcgccga ggccactttt tgccgcagcc cggtgcgaat
     2461 gttaatggcg gcaaattccg gcgtctcttc ttcgccgctg cgcgcgcttt cccacggaaa
     2521 tagcgcgccc tgccagccgt tgcgtcgcgc tttctcctgc gcgcctggca agttgtgcca
     2581 gcgataacgc agtaaacttc gggcaaccgt cggatcgcta aacagatgaa acggtaacaa
     2641 aaatacttct gtatcccaga aaacgtggcc tttgtagcct tcgccggtta agccttttgc
     2701 cgcaatgctg ctgcgctcgt cgtgggcagg cgtcatgatg cgcagatgat aaagcgcata
     2761 atctaacgct tgctgatcgt gcacttcgcc gccatttacc gtgatacgac gtttctgcca
     2821 ccattgacgc cagttttctg ttgatactgc aagaagttgg tcgtaactct gctgcgcgca
     2881 catttcaagc tggcgaagcg acgcgctgcc ccactcgtct aaaacggctt gcctgtcatc
     2941 ccgccagtcg atccacacca gtttttgcaa cgtcactgtc tcgcctgcat gaagctgcgc
     3001 actggtatgt tgcagtaaac ggcgctcttt ggcggtatag cattgctgca catcaccgct
     3061 caccttacag caacagctga tggccacatc actgcgtcca tcctgggtgg tgtagatccc
     3121 ctgcatcaaa tgctgaccaa acacccgcac ctgggtttcg tcgagatgct ggcgaccgtg
     3181 gttggtctgc gtggcgtcaa tgcctgttga aatcagcact gacgcgtcgg cgtccagtgg
     3241 cgtaatagtg atttccagca caatgagcgg cagttggtct gccgaaacaa agcgacggct
     3301 ggcgatggtg taacctgtgc cgttgctggt acgccagaca acgctacggc gtaattctcc
     3361 actggcaaag tcaagttcac gctgccaggc ttcgcgggat aacgagaaaa cctcaccatt
     3421 tatggcaatc tccatcccca cgacatcagg caggttcacc agttcgttga tttcaccttt
     3481 tcccgcccga tgatacagcc ccgccagata catccctcgc gtctggcgcg tgtaatcttc
     3541 ttcatggctg gcgcgaagcc caagatagcc gttcccctgc gccatcagcg atgcatactt
     3601 gttcagggta tgctggctga aatggggttc tgataacgtt actggcctgg tcataattcc
     3661 acacgcgtcc ctgtttctgc ggattgatac aacgccgcca ccagttgctg gatgatgtac
     3721 ccctgcttgg cgtcggctat catcacgggc ttgccctgta cgtgattgat aaaggcttcc
     3781 atgctgcgca aatggcggtt gtcgtctgcc attttccgtt gcatcagcgt cattaattca
     3841 ccgttgttat cggtgtagat atgtgctgga aacagcgtcg caccagcttt atcaccacaa
     3901 aagctgacgt tcataatcga ctgttcgcgg atgttgagtg caaatgacgt ttccagccac
     3961 agaatgccgc cgttatgaaa ttcaatggtg ccaaacagcg aatcttcgac gctgtaagtt
     4021 gccggatccc actcaccaaa ttgaccacag ctcttttgcg tgccgatctt ttgaaagcta
     4081 tgcgcattca cgcttttcac cgccggaaag cccagcacat acatcgcagc atccagcata
     4141 tgaatgccga tgtcgatcag cgggccacca ccctgcagtt ctttattggt aaagacaccc
     4201 cagccgggaa cgccgcagcg acgcagggcg cgggcggtgg taacgtaaat ctctcccaaa
     4261 acgccgttgg tcacctgttc acgcagctgt tgcgtatcga gcgcaaaacg atggtgaaag
     4321 tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc acatttcccg cgcctgttct
     4381 ggcgtcatgg cgagcggttt ttcgcacatc acatggcagc ccgcttccag tgccatcagg
     4441 gtatgttcgt aatgaaaacg gttaggtgag cagacgctaa ccacatcagg tttcaccgcc
     4501 agcagcatgg cctgcggatc gtcccacacg gatgcattcc cgtatttttc tgccagcgcc
     4561 tgcgcctggg aaaggcggct gtcacagaca gccaccagtt ccagatcgtt gcgggtgcag
     4621 tagtacgaag catgaacctt atccgccacc tggcctgcgc ctattatcgc gacccgcagc
     4681 ggagagcttg tcattgcact tttcacaaag acctcttagc aggtacgcaa ccaggccaac
     4741 gaatcacggt acgcctgggc aggatcttcc gcccggatac gcccttcgta cactacatag
     4801 ccctgatagt tatccgcgcg cagctgttca aacagcgcgt ggaaatccag ggtgccgctg
     4861 cccggctggt agcgatggtt atccgcaata tgcacatgac cgagcaggtc gcggttgtca
     4921 tgcagcgcct gcgccaggtt atcttcttcg atattcatgt gatagaaatc gccgataatc
     4981 tgtacatgtt taagatcgtt ttcgacgatg taacggcggg catcggcgag ggtgttgatc
     5041 atatgatcct gatagcggtt taacggttcg agatacacca ctgttccggt acgcgcggcg
     5101 acctgttcca gtacgcgcag ggaatcactc accattttgc ggtcgccgtc caggctacgc
     5161 ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc acgctgccgg aacgacgata
     5221 cctttatccc ccacttctgc cagcgcttcg agaatgcgtt cgatctgctt taagccatta
     5281 agacgacgct cttcgataaa gtcgccaatc caaccgtcat agccaccgca ggcggtggtc
     5341 accggtaaac cggtttcttt gattgctgct ttgacttctt cgagattgtt gaccagcagt
     5401 ttgccgtcaa tctcaaaacc atcgaagccc atctctttga tataacgaaa tttctccaga
     5461 atgttttccg gaaagaatgc ctgattctgt gtgcctattt tcattttttc attcctttga
     5521 attaaaacgt aacgcccatt ttgatgctct gttccggatg ctgatcgaca tactgcatgt
     5581 agctttccgg gctattggca aaggtcacta ccgggtcgat taaatcttcg caattgagat
     5641 aaccgttcat cagcagttcc cagcaggttt cttcaatacg cttacggctc cagcgcggat
     5701 aatccgggtt cggttcgctg cacgcgcggg agaaaacaat tttggcgtta ttgaaatgcg
     5761 cttcgcgtcc gaggttaaaa ccttcagcaa atggtttggc aaacgcaaca taggagatgg
     5821 tgccaccgta ggccagaccg cggaacgccg attgcagcgc gtcggcatag ccgctggttt
     5881 cgataatcac atcggcaccc tgcttgccgg tcagcgtttt gatctctttg cctacgtcag
     5941 taccaatggg gttaaggcag aaatccgcgc cgtggcgacg ggcaatatca cagcgatgag
     6001 cgataggatc gacgccaatc acgaccgacg cgccagcacg tttagccagt tgaatggcaa
     6061 tttgaccaat cgcgccaagc cctaccacca caacaaaatc acccacgcgc acgttggcat
     6121 cgcgcacgcc gctcatggca aactgcgccg ggtcgtaaca aacggcgttt ttccacgagc
     6181 tgccttccgg cattttgcgc agcttgtagt tattcaccgc gttaatgatg accgtctcgg
     6241 agagcgggcc gtagccgcat acgctgtcgc ccaccgcgta gtcggtaacg tcgctgccgc
     6301 actcgataat gtcgccaacc accatgtttc caagctggaa tttgccaaac tcaatgccgc
     6361 gcggcgcatc tgccggacgc ggagtgaaca tctgccattc gccgttaaag tcttcatcga
     6421 taaacgggct ggcggcgcgg aagtcgacca cttccgttcc gtgtttcggt gcgccgaaac
     6481 gcacgcggac cttcacttca ttagctaaaa tcgcccgatc ttcatactca accagcgcag
     6541 caacacgcgg tgctgtggct actaactttt tcatttaaaa ctccttgtaa agaaactggc
     6601 cgctttagcc cttcacgcca ccggcggtca aaccactctt aataaaacgt tccgaaagcg
     6661 catacataat caccaccggc aatgcggtca ccagtgaggc cgccatcatc cgtccccaga
     6721 tgtaatctgg cgtactgaac agcgcgttca ggcccaccgg taaggtgaaa ttgccggcgc
     6781 tggagaggaa aatcgacgca aacagatagt cgttccacgc caccataaag cagtagacga
     6841 acacggatat cagcccggac atcgccaacg gcacggtgat gcggaagata atttgcagac
     6901 ggttgaggcc atccatcatc gccgcttctt cgatctcatc ggggatggtg tcgaagtagc
     6961 ttttcagcat aaacacggcg gtgggcagag tttgcgtcac catggtgatg atcagcgcca
     7021 tctcagtcga cgtaatgctc cagcgtccac tgctgcggca gcagcgtagg atgcagtgag
     7081 atcgcctctt tcgcgccctt gaacgaggtc atcagcatca caaaaaatgg aaacagcgtg
     7141 atgatgagaa acagcgccag cccgcagtaa aaaccgatgc gactgagtgt gcgtttattt
     7201 gttgccattg aggttcaccc gtttcctggt cagcagaatg acagcgaaaa taatgatgaa
     7261 gagcaccacc gaaatcgccg ccgctttccc taagtcatta aaggcaaagg cggttttgta
     7321 gagatagacg ccgagaatat cgaccttggt ggtcagcaga taaacatcag caaacatgta
     7381 gaacatccag atagtgcgca gggtcaccac cgtcgccagg accggcataa ttgcgggcag
     7441 cgtgacgatg cgaaaccgct gccaggcatt agcgccatcc atctctgccg cttcgtacag
     7501 cgatttgtcg atggtctgca aaatcgccag aaacgagata aaggcatacg ggaagtagcg
     7561 ccagatggcg aacagcacca ccagcacaaa gctactgccc ggattatcga accacagcgg
     7621 tgcctgctca tagagatgca gaagatcgac gccgaggtag ttcacaatgc cgtagccgtt
     7681 gttgaacatg tatttccagg caaacaccag cgaaatggac ggcgttacgt aggagaggat
     7741 caccagcgaa cgcgcggttt tgcgcaggcg gaactcgcgg ttgaaaaaca tcgccactgc
     7801 cagcccgagc acggtgctgc ccgccaccac cagcgcggta taccagaccg tcatccacag
     7861 cgagtgccag aagccgggat cggagaggat acgcacatag ttactcaccc caacaaaggt
     7921 tgactcgatg ttgggattga gcggcagacg taaaaaactg atttcgatat tcgacaccat
     7981 tggccaggcc accagaccac ccagcagtaa taagctgggc gcgagaagca gcagcgcaaa
     8041 gggcatatcg gaacgacctg aaaaaagcct gttcattgcg gattcctgtc ctgggtttag
     8101 tgctgttcga tcagttcatc cagctttttc tggctcgctt gcagcgtgcc agggagatcg
     8161 gctttaccca cggtgacgtt atgcaccatg gagctcacca cgccagaacc cgtcacatca
     8221 cccatgcggg taaagttttt atcccctact gcgccaaaga cctgaatatt tggcagttca
     8281 ccgattagct gattcggtag ttcacccagc gccttaataa cgtcgttgtc tttccaggtg
     8341 gcggtagtga caaccgcttt attcaccggc agcgcagcac ctggcgacat catcacccag
     8401 tcggcaatgt tgtctgcctg ctccataaag gtgacaaatt tttctgctgc ttccgtctct
     8461 tcggcctttt gcccggcggt aatggtcagc gaggtcaaca tgccgtagac cgcagagttt
     8521 ttctcggttg gcaccacgaa accgacgttt ttcgggtcgc cttctttaat tacagccgga
     8581 aggatatagg tggagtaaat cgccatcggc gcggtgccgt tcataaaggc gtctttcact
     8641 tccatgatgt cgttagaacc cggcatgtcg ttagaacccg gcatagtgtt ggcagcaagg
     8701 tcgcggtaat aggtcagtgc ctgcatcatc tctggtgtat caagggtgat tttgccttcg
     8761 gcgttaaaga cattagcctg gttggataac gcaaactggg agaaggattg ttccgtcaac
     8821 acgctttctg ctgtaggcag cgcaatgccg tattttttat tcgccgggtc attcagtttc
     8881 tgtgcaacgt ccagtagctg ttgccagttt ttcggctcct ccagccccgc ttttgccagc
     8941 acatctttgc gataccagat gccgccaatc caggcgctga cgggaacacc ggtccaggca
     9001 ctcccatctt cggtgcgcac aatacgcagt acgccatcgt aaaacgcgcc ttcaccaacg
     9061 ttgctgatga ccgtggcaac cgtcttgcga tcgataagct gctctttatc cataactttg
     9121 gcgtagtcat ggctggtttc gatcacttcc ggcagcgagc cgctacgtga aagggtgatg
     9181 actttggtgt tataagcgtc ctcttccacg ggcacttgtt taacggtgat acctgggttt
     9241 tctttttcaa aacgggcaat caatttgctg ataactgcct gacgctcctg ctccaccgaa
     9301 gaatgcataa attcgatgga tacatgggtt ttattttctt ctttacagcc tgaaatcagg
     9361 gcgcaggaaa ccagtgctga taacagcacg atttttgatt taatcatgag atgttccttt
     9421 ttatttaatc cacataacct gttaggggcg cagcgttatt tcattaccca atagatcaac
     9481 ttcactaatc agatcccgac catgcagatt agtaatattt acatgctgaa tatttttact
     9541 gacattaaac aatccagtca gacaatcacc atcagcgtta cttctttgaa tacgcattac
     9601 ggatgaatta atcgtatcga tggtaaaatt attatccgga tgaaactcgt tatggctgcg
     9661 acgaagtgta attaaacgcg acaactcatg atataccgca tgccttaatg tagcttcatc
     9721 gttcagttct cgggttatct ctttactgtg atatttttta cggttaatcg cacggttata
     9781 tccgagtttt tcgacacctg cgtaatcatt acgcgagcca agaatacttt gaatatatat
     9841 cgctggcaca ccggggaaac ttaacaaaat cgcatgggcg agaataaacc tggcgcaacg
     9901 ttcttcatcg ctactctcac ggcggcttaa cgcatccata tgtgagaggc gaggaagtta
     9961 aaccaggtgg tgttgctgga aggtagtgtc aggttttgcg cccacgcaca aagcgcctca
    10021 acgttctgtt tttgcaccgc atgcagcacc agtggcggca gcgagaactg gtacaccata
    10081 tgtgcttcgt catcgcctgc gccaaagtaa gcaatgttgt ctttatgcgg aacattggtc
    10141 tcggtaatga tcactgtacc tggcgcaacg ttatcaataa tcgaccgtaa cagtttgata
    10201 atcagatgtg ttttttccag atggatgcag cttgttcccg gctctttcca cataaagcca
    10261 acggcatcca ggcggacata ctcagcacct ttcgcaaggt aacagagtaa aacatccacc
    10321 atcgccagca acacttcagg gctacggtaa ttcaggtcaa tttgatcgtc actaaaggtg
    10381 gtccataaat ggcgcgttga atgatcgcgc atctggaatg gcgttaataa cggtaacgca
    10441 cgcgggcgag tgacggcgct gagatcggtt tgcggatcaa ccgcaataaa aaaatcttca
    10501 aaacctggct gctgttgtaa atagttttta aaccattcac tttttgccga catatggttg
    10561 cagacaaaat caaacattaa atgactgcat tcaccgagtt gctgaatatc ctgccactcc
    10621 cccgcttcac tggcgacctg atgataatca attaccgaaa acccatcatc agatgaacat
    10681 ggataaaaag gtaataaatg aacatgtgaa aaaatgcttt gcagccattg atggtaaaac
    10741 tgattaaatg tgggtaatgg ttttaaatca ttgctgtgaa attgatcggc ataggtaatt
    10801 aacacgacat cactttcatc ccagtgtttt ttacgtcgct gtgtaattaa tcgtttcgca
    10861 ctctcaagac gcgttacaag tttctgtaaa tgagttgcgg taaatgttcc accgtagatt
    10921 tcgtccaggt aatccgtaat tttctgtttc atgttgcctt tttgtggtag cggtcccatg
    10981 aaacgagaga ctactccgca gaaaataaac tgtcaattga gcacaagggt tttagtcacg
    11041 caggaaatca ttttttacca gggaaaaagc gtatttgtga gctgacgcaa ataatctatg
    11101 gggatgtgaa taaggtgtga cagaaaaaaa acggcgcata agcgccgctc atggtgaatt
    11161 cttttaacct ttgaccttcg gcattgcgat gtctttcagg ccaccggcgt tctcaacgtg
    11221 ggtatatccc atctcgctaa ggatctcttt tgcttgccct gactggcgtc cggcattgca
    11281 atacactttc acggtgtcgt ttttatccgg aacggcggtg gcaatgcgct ctttcacttc
    11341 cttcagggga atattgatgg ccccctgaac gtgctcttgc tgatactgct ctggaacacg
    11401 aacatcgatc cagtgttcag cggcgaaaac gggcagtgaa aacaccagtg ccagagcaag
    11461 taagcctttt ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg
    11521 cctgagattt aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta
    11581 ttagcagccc gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg
    11641 cgagcaactg attttatcgc ccagcccgct acacccgccg ggccatagcg cagtgcggta
    11701 agaagtacca gcttgcctgc taatttgaaa ccaggcttta ccttttgccc ggcctgttgc
    11761 cagcgagtat tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat
    11821 cggaagtgac ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt
    11881 cgacttcatc cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg
    11941 gcattggatc aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac
    12001 cgaagaaaat cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt
    12061 tggcaatccc ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataattttt
    12121 tattgagatt aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat
    12181 agattccagc gcctgaatac gttcgcgcat ccgttttgct tcatcagcca gttgcgctaa
    12241 tcgctgctgc tcactttgcg acaattcatc gcgaccagaa cgattgctgt aatgcagcca
    12301 taaccagatc ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc
    12361 gctcataagt actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat
    12421 tattgattgt cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca
    12481 tcggctttca gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt
    12541 tctgcttccg cttccatctg gtcaattcga cgttcgaaag attcgaaacg agccattgct
    12601 tcatccagtt tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga
    12661 tgacgtaaca tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg
    12721 ccaatctctt tcttcatgcg tgccagcgta tcgttcacca gcgtcacttc atgttccagg
    12781 gacttaatca gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct
    12841 ctctctttca gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct
    12901 tgttcaatac ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt
    12961 acttcaacca gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct
    13021 tccgctttct ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa
    13081 atacccataa tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat
    13141 acaatttgcg tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac
    13201 agcaacatgc caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc
    13261 gaatacaaag ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt
    13321 tcgcatctcg caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa
    13381 gagctgattg ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc
    13441 cttaactgcg cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg
    13501 ggggcgttta ccggtgcgca aaaacgtcat ccagggagat ttgaacgtgc cgacggcggt
    13561 acgctatttc ttgatgaact cgctacggca ccgatgatgg tgcaggagaa attattgcgc
    13621 gtgattgagt acggtgaact ggagcgcgtt ggcggcagcc agccattgca ggtgaatgtg
    13681 cggttggtat gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg cacttttcgc
    13741 gctgacctgc tcgaccgact ggcttttgat gttgtacaac tgccaccact gcgcgagcgc
    13801 gaaagcgaca taatgttgat ggcagaacac tttgccatcc agatgtgtcg ggaaatcaag
    13861 ctgcctctgt tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg
    13921 ccgggaaata ttcgtgaatt gaaaaacgcg gtggaacgtt cagtgtatcg ccacggcacc
    13981 agcgattatc cgcttgatga catcattatt gatcccttta aacggcgtcc gcctgaagac
    14041 gctatcgccg tttcagaaac cacctcgctt ccgacgctgc cgctggattt acgtgagttt
    14101 caaatgcagc aggaaaaaga gttgctgcaa ctcagtttgc aaaaggggaa atataaccag
    14161 aagcgcgcag ctgaattgct ggggttaacc taccatcagt tccgcgcgtt gttgaaaaaa
    14221 caccagattt agcgaagttg ggcaatgcga ttcgccggat gcggcgtgaa cgctttatcc
    14281 ggcatacata atcaacgtcg tttgcaggcc tgataagcgc agcgcatcag acattattgc
    14341 gttgggctat taatcgctca gcgcatcctg caaaattttc attgccgcat cgaattgcgc
    14401 atccgggatg gtcagcggat acaggaagcg aatcacgttg ccgtatgcgc cacaggtcag
    14461 caggagcagc ccctgcgcca gcgcgcgttg ctggattttc tgtgcaatcg ccgctgacgg
    14521 ctcgcccgtt tgcggatcgt taaactctgc cgcaatcatc gaccccaggc cgcgtaccgc
    14581 agcaatggcc ggaacgcttt ctttggcatc aatcaacgtg tttttgagac gctggcccag
    14641 ttgattcgcg cgttcgcaga gtgattcttt gttgataata ttgagcaccg cgtgcgcggc
    14701 agccaccgcc agcgggttcc cggcgtaggt gccacccaac ccgcccggcg cgggcgcgtc
    14761 cataatattc gcgttaccga ccacgcccga aagcggcatc ccgcccgcga ggcttttcgc
    14821 catcgtcatt aagtccggct tatcggcgta atgatccatg gcaaacagtt taccggtacg
    14881 cgcaaagccg ctttgcactt catcggcaat catcacaata ccgtgctcgt cgcacaggcg
    14941 gcgaatggcg gcaaccagct cttttggcgc aacgttgaaa ccgccctcgc cctgcaccgg
    15001 ttcgaaaata atggccgcga cctgcttcgc ttcgatgtct gatttaaaca agcgttcgat
    15061 ggcgtcgagg gagtcctatg ttgaaatgcc gtgtaaatct gacggataag gtacgtgata
    15121 caccgaacca gggaacgggc cgaagccgat tttgtacggc gcaacttttc cggtcagcgc
    15181 catggtcata tatgtacgac cgtggaagcc accgctaaac gcaatcacgc cagggcgtcc
    15241 ggtatgggcg cgagcaattt tcaccgcgtt ttccaccgct tccgcaccgg tagtgaagaa
    15301 cgcggttttg gcctgcccgc tcaccggggc aagggcgttg attttctccg ccagggtgac
    15361 gtagctttca tacggcacaa tctgatacgc ggtgtgggta aactgttgca gttgctgctc
    15421 caccgccgcg accagatcag ggtggcgatg tccggtattc agcaccgcaa tgcctgcggc
    15481 gaaatcgatg tactcgttgc cctcaacatc cttcagagta gcgttttcag ccgactgggc
    15541 gaagaagtta cacatcacgc caaccccgcg tggggtagca gaaagacgac gctgatggaa
    15601 ttcattgttg ctcatgtttc acaccttttg actcttcact gttaatcgga aattaaacgc
    15661 ccagacgatc gcgcaggctg taccaggctg cgcccatcgc ggtaaacggt actcgcagcg
    15721 tgcgcccgcc ggggaacggg taatacggca gattggcgaa ggcatcgaaa cgttcggcgt
    15781 cgccgcgcag cagttcggca atcaaacgtc cggcgagatg agtacaggtg acgccgtggc
    15841 cgctgtaacc ctgcatgtaa tagatgttgg tatcaaggcg accaaactgc ggcatgcgcg
    15901 acagggtcag caggaagttg cccgtccagc ggtagtcgat tttgacgccc ttcagttgcg
    15961 ggaaggtttt cagcagtttc ggcacaacaa gacgctcaac gtcatccggg tcgcgcgcgc
    16021 cgtagaccac gccgccgccg tacagcaaac ggttgtcggc ggtaagacgg tagtaatcaa
    16081 gcagataatt acagtcttcc acacagtagt ttttcgggat cagcgaacgg gctaaatctt
    16141 ccgacagccg ttcagtggtg atcacctgcg tgccgcacgg catgctgcgt ttcgccagtt
    16201 ccggctctac tttatcgccc agatacgcat tcccggcgac aatcacatac ttcgccgtca
    16261 cctgaccttt ggcagtgcgc accaccgctg gcgtggtgtg ctggatctgc gtcacggcag
    16321 aaagctcata cacgcgcccg ccgttcaggc ggatggcatc ggcttcacca atggcgaggt
    16381 ttagcgggtg aatatgccca ccgctgtgat ccaacagtgc gccagtgtag cgatcgctgg
    16441 cgacctcgcg gcgaatggcg ttagcgtcca gcagctccag ctgtttattg ccgtagcgtt
    16501 cccagttctc tttctgctct tcgagtgtgg cgagctgttt atcattcatc gccacaaaca
    16561 ggccgccggg gcggtagtcg cagtcaatct gatagcgttt gatacgttcg cggatgatct
    16621 cgccaccctc gaacatcatg ctgccgagca tacgggcggt gtccatgccg tagctttttt
    16681 cgatcacgtc gatgtcgcgg ctgtaggagt tcacaagctg cccgccattg cgcccgcttg
    16741 cgccaaagcc gatgcgtgag gcttcgagaa ccactacgtc aaagcccgct tccgccagat
    16801 gcagcgcgga ggagagcccg gtatagccgc cgccaaccac gcaaacgtcg caggtgattg
    16861 actcattcag tgtgtcgaat ggtgcatatt tattcgcact ggcggcgtag taactgctgg
    16921 tatgttcggt catttcaggc ctccaggctt atccagatgg ttttcagttc agtgaatttt
    16981 tcaagggcat gcagggattt gtcgcgaccg ttgccgctct gcttatagcc gccaaacggc
    17041 acggtcatat cgccgtcgtt gtagttattg acgaagacgg aaccggcttt caggcggcgg
    17101 ctcatacggt gcgcgcggga gaggtcgcgc gtccataccg ccgcgccaag gccgtactgg
    17161 ctgtcgttgg caagctgtag cgcctggtct tctgatgtga aacgcgtgac caccagcacc
    17221 ggaccgaaaa tctcttcgcg gcttaaggac gcattcgggt caacatccac aaagatggtc
    17281 gggccgatgg cggcagccag ctcagcgtta cgaccatcca gcagcagttg ccctttgctt
    17341 tcgccttcac gaataaagct atggaccgag tcggcgtggg cgcaatcgat taaggtgccc
    17401 atggtggtta cggtatcaag tggatggccc ggctgccagt tttgcgcctg ctgttttaac
    17461 agggctaaga attcatcggc gatgctctct tccagtaaca aacgcgttcc ggcgatgcac
    17521 acctggccct ggttgtagaa gatgcctgcg gcggtggcgc ttgccgcttt ttgcagatcc
    17581 ggacaatcgg cgaaaacgat gttggcgctt ttgccgcccg cttccagcca gacgcgtttc
    17641 atgttgctat cacccgcgtc tttcagcagt tgtttacccg tacgcgtgga gccggtaaag
    17701 gcgatggcat cgatatcgtt atgacgcgaa agcgcctgcc cggcttcatg accataaccg
    17761 ctcactacgt tcagcacgcc atccggcagc cccgcttctt cttttgccag cccggcgaga
    17821 cgaatcgcac tgagtggtga tttttcagac ggttttagaa tcacgctgtt tcccgccgcc
    17881 agcgccgggc cgagtttcca gcaagtcagc agcagcggga agttccacgg cacgatggcg
    17941 gcaatcacgc cgaccggttc acgcacgatc atcgccagct catggctact ggtggtcgcc
    18001 acttcgccat acactttgtc gatcgcttcg gcgtaccagc gaatggcgcg cgccgcgccg
    18061 ggaatatcat cacgcagact gtgacgaatc ggtttgccgg tgtcgagagt ttccagcagt
    18121 gccagctctt cggcgtgggc ttccattaaa tcggcgagtt tattcagtac cgctttacgt
    18181 ttagccggag aagagagtga ccagtcgccg cgttcaaata cgccgcgtgc tgcgctcacc
    18241 gcatggtcga tatcgacgct cttgccgcgg gcaattttcg ccagcggtgc ctaggtgacc
    18301 ggatcaacgg tttcaaaggt ttcattttcc gccgcagcag tatattcacc gttaataaat
    18361 aagcggtttt caatggcgag acttaacgct ttatcctgcc agtaagccag atgatgaaaa
    18421 ttcattatga ctcctgtttc acgtctatca gatatatgca gtttgtgaac gtcagggcgc
    18481 agagatcccc gggccgcatg cccgtttatt tctggcgaca ttctttatgg attgccgggc
    18541 gcattgcgct tacccggctt acagaacaaa aagattaaaa cgtggtgggc gtatgggcgc
    18601 tgataattcg gcaaatacct gccgacgtat tactgaaact gtgcgggatg ccggtattaa
    18661 tggcataact ttgccccgcg acgaggtggt aatcctgacc attaatcgtc agaacaattt
    18721 caccttccag tactgtgcct atttcctcac cctgatgctt aattctttcc ccagtggttg
    18781 tgcccggctg gtacgtttca aagatcatcg ccagcgtgcg attcgggtta ccgttatgaa
    18841 ccagcttcat tgacacaccc tgactgccca tctcaattaa gtcgtcctga ttaatgacga
    18901 cctgcggctc atcaggtttt tccggctcgg aaaagaattc cgagagtgac agaccataca
    18961 ccttcagcag cttttgcagc gtactgatgg cagggctgac tttatcttgt tctatcgtac
    19021 tgatagcact gtgagtcagc ccggagagtt cggcggcacg acgttgtgaa agcccctgct
    19081 gctggcggat ttccgacaag cgttttcctg gcgccagtcc ctcatcactc atatttgcat
    19141 ttccttaaac tgtagtggtc agagtcgctg tttttcagcg atatggtgct gacaagcggt
    19201 gataaagccc tcgaacaata tacgcgaaag cgcgtactcg ctactgttcc attccgggtg
    19261 ccactgtacg cccagcgcaa aaggatgatt gatgacgcta accgcctcaa ctaagccatc
    19321 cggcgagcga gcttcaacac gcaaccgtgg gctaacgacc ttcgcccctt gtccatgtag
    19381 agagtttacc caaaagttgc tacattcagg taacaacgca gacagtaatc ccccctcttc
    19441 aacctgaact tcatgcgacg gtgcatattg ctgttccacc ggcagttcgg gatcttcccg
    19501 atgttccagc aattcaggct gttcgcacag cttgcgatgc aacgaaccac cggttgccac
    19561 caccagttct tgtaaacccc ggcagatggc gaaaatgggg atgcgccttt cgagtgcggc
    19621 attaattatc gccatgctca gaagatcacg cccgggatcg gcgtcaggct catcgccgtt
    19681 ttcaccatat aggtgcggct gcacattgct gggactacca ggaagataaa tgccatcgag
    19741 tttcggcaaa agttgttcaa gtaacgacgg ttccgccagc gcatgtggta gcgcaattgg
    19801 caagccgcct gcgtggatga tggcattcag gtacttttct tgcagagtct gggtcgcatg
    19861 acccttaagc ctgttcctgc acattacgac accgataacc ggattgttca ttatattttc
    19921 catgctcaat ctcacaaagt ggactaaatt atcgccatta ctgcgcttta aatgaccttt
    19981 atgttcaata ttttttcaat ctagcagtgg gttttcattt ttgcaaactc aatttaacat
    20041 ttgacaaaca tttagtttgc atacagattc gaatggtggt cattatattt tacgctttga
    20101 taacgagcgg aaaacaaacc aaaggcgaag aatcatggaa accaatatcg ttgaagtaga
    20161 gaactttgtt cagcagtcag aagagaggcg gggtagcgcc tttacgcagg aagtgaaacg
    20221 ctacctggag cgctacccga atacgcaata tgttgatgtt ttacttactg atttgaatgg
    20281 ttgcttccgt ggaaaacgta tccctgtttc aagcctgaag aagctcgaga aagggtgtta
    20341 tttcccggcc tcggtatttg caatggatat tctgggcaac gtagtagaag aagcaggtct
    20401 gggtcaggaa atgggcgagc cggatcgtac ctgtgttcct gttctcggtt ccttaactcc
    20461 ttctgccgcc gatccagagt ttatcggtca gatgctcctg accatggtcg atgaagatgg
    20521 cgctcccttt gacgttgagc cgcggaacgt tcttaaccgc ctgtggcagc agctgcgcca
    20581 gcgcgacgct gaagggaatc tgcaaccgcc ctgcgcgcct ggcaccgatg accgcaatac
    20641 acaaagccag gtttactccg ttgataacct caaccacttc gccgacgtgc tcaatgatat
    20701 tgatgaactg gcgcagttgc agctgattcc ggcagatggc gcggtcgctg aggcctcgcc
    20761 gggtcagttt gaaatcaacc tttaccatac tgataacgtg ctggaagcct gcgatgatgc
    20821 gctggcacta aaacgcctgg tgcgtctgat ggcaggaaag cataagatgc acgccacttt
    20881 tatggcgaag ccgtatgaag agcacgcggg cagcgggatg catatccata tcagtatgca
    20941 aaataatcgt ggcgagaatg tgctttctga cacagaaggc gaagattcgc cgctgctgaa
    21001 aaagatgctc gccgggatga ttgacctgat gccgtcgtcg atggcgttgc tggcaccaaa
    21061 cgtgaactcg tatcgccgct tccagccggg aatgtatgtg ccgacgcagg cgtcgtgggg
    21121 ccataacaac cgcaccgtcg ccctgcgtat tccgtgcggc gaccgtcata atcaccgcgt
    21181 ggaatatcgc gtggcgggtg ccgatgccaa cccatatctg gtgatggcag cgatttttgc
    21241 cggtattttg catggccttg ataacgagct gccgttgcag gaagaagtcg aaggcaacgg
    21301 gctggaacag gaaggcttac ccttcccgat tcgccagagc gatgccctgg gtgagtttat
    21361 cgagaatgat cacctgcgcc gctatttagg caaacggttt tgccatgtgt atcacgcctg
    21421 taaaaatgat gagctgttgc agtttgagcg cctcatcaca gaaacagaaa ttgagtggat
    21481 gttgaaaaac gcgtaattcc ccttgcgggg ttgtttcatc ggcctgagcg gagccaggcc
    21541 tgacatttgc tggtaatcca gacatttccc ggatgtcgcg ttatggagcg caggcggcaa
    21601 cgggcttccg ttaacgatta agactgtgac gaggaaatga gatgatgccg atgattaaat
    21661 ccccgcatgg cgagggaggc tgcgtatgcg cccctcctgc tactgactgg actccgcccc
    21721 ctttgcttcc cttgctaaac aggtttgatt tcaggtcgac acgaccgcaa accttattac
    21781 gcaggggagg cagcaattat ggctattaat tcaccactga atattgctgc gcaacccggc
    21841 aaaacccgtc tgcgaaaatc actgaaattg tggcaggtgg taatgatggg tctggcctat
    21901 ctcacgccga tgaccgtatt tgatactttt ggtattgtct ccggcattag cgacggtcac
    21961 gttccggcgt cctatttgct ggcgctggcg ggcgtgctgt ttaccgctat cagctacggc
    22021 aaactggttc gccagtttcc ggaggccggt tcggcctata cctacgcgca aaagtcgatt
    22081 aacccgcacg tcggatttat ggtcggctgg tcatcactgc tggattatct ctttttgcca
    22141 atgatcaacg ttttgttggc gaaaatctat ctctccgccc tcttcccgga agtgccgccg
    22201 tgggtgtggg tggtaacctt cgtcgccatt ttaaccgccg cgaatctgaa gagcgtcaac
    22261 ctggtcgcta acttcaatac cctgtttgta ctggtgcaaa tctccatcat ggtggtgttt
    22321 atcttcctgg tggttcaggg actgcataaa ggagaaggcg ttggcaccgt ctggtcactt
    22381 cagccgttta tcagcgagaa cgcgcacctg atcccgatta ttaccggtgc gacgattgtc
    22441 tgtttctcgt tcctcggttt cgatgcggtg accacgcttt cggaagagac gccggacgcc
    22501 gcacgcgtga tcccgaaagc catcttcctg acggcggtat acggcggcgt tatctttatc
    22561 gcggcgtcgt tctttatgca gctgttcttt cccgatatca gccgctttaa agacccggac
    22621 gccgcactgc ctgaaattgc gctgtacgtc ggcggcaagc tctttcagtc gattttcctc
    22681 tgcaccacgt ttgtgaacac attagcgtct ggcctggctt cacatgccag cgtgtcgcgt
    22741 ctgctgtatg tgatggggcg tgacaatgtg tttccggagc gcgtgtttgg ctatgtgcac
    22801 ccaaaatggc ggactccggc actcaacgtc attatggtcg ggattgtcgc gctgtcggcg
    22861 ctgttctttg atttagtcac cgcgacagca ctgattaact tcggtgctct ggtggcgttt
    22921 accttcgtga atctgtcggt gtttaatcat ttctggcggc gtaaaggaat gaataaaagc
    22981 tggaaggatc acttccacta tttgctgatg ccgctggttg gcgcgctgac ggtgggtgtg
    23041 ctgtgggtta acctagagtc aacgtcactg acactcggtc tggtatgggc ttcgttaggc
    23101 ggcgcatatt tgtggtatct gatccgccgc tatcgcaaag tgccgctgta cgaaggtgac
    23161 agaacgccgg tgagcgaaac gtaatagccg gatgcgcggc gcattatcct gcctgagggt
    23221 aacgcgccgc gccattaact ataaaaagat gtataacccg gatcacgagt gctatcatcc
    23281 gcccttatgc ttagcgcatt aaccccaggg gcaaataatg aaccacgata tcccgctaaa
    23341 atattttgat attgccgatg agtacgcgac cgaatgcgca gagcctgtcg cagaggctga
    23401 acgcacaccg ctggcccact acttccagtt actgctcacc cgtttgatga acaatgaaga
    23461 gatcagcgaa gaagcccaac atgaaatggc tgctgaagcg gggattaatc ccgtacgcat
    23521 tgatgagatc gcagagtttt tgaatcaatg gggtaatgag taattattta attgcagacg
    23581 atcggtcagt atcgttgtcc ggataaagcg ttcacgccgc atccggctaa caatgacacg
    23641 cactgcagcg tgctttaatg ggtttctcac gatcgtaccg ctctgctcct cgctaagttg
    23701 ttctgtgagt tatagttata gagaaatcac gggtacgaat gttgaacaga tggcgacgcc
    23761 acctgttcaa cgaccaacca cacggccctc attacgaccc ctgaacacaa acggtaatgg
    23821 caaatgtcat taaatattag taagatagcc tctttacata aaatccctta atattatgcg
    23881 ccaggtatta tcgtctcttt tggtgattgc tggacttgtg agtggtcagg caatcgccgc
    23941 gcctgaatcc cccccgcatg ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg
    24001 gcaagtcaac acctttaacc catccaaagc gagcagtggg ttaattgtcg atacccttgc
    24061 cgcccagttt tatgatcgac tgctggatgt cgatccctat acctatcgcc tgatgccgga
    24121 acttgccgaa agctgggaag tactcgacaa cggcgcgacc tatcgcttcc acctgcgtcg
    24181 cgatgttccg tttcaaaaaa ccgactggtt tactcccact cgtaaaatga atgccgacga
    24241 tgtggtgttt accttccagc gaatttttga ccgcaacaac ccgtggcata acgtcaacgg
    24301 cagcaacttc ccctacttcg acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa
    24361 actggataat cataccgttg agttccgact cgcccagccg gatgcttctt ttttgtggca
    24421 cctcgcaacc cattatgctt cggtcatgtc ggcagaatat gcccggaagt tagagaaaga
    24481 agatcgccag gagcaactcg accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga
    24541 ataccgcgcc gggcaattta ttcgcctaca acgtcatgat gacttctggc gcggtaaacc
    24601 gttaatgccg caggtagtgg tggatttagg ctccggcggc accggacgtc tgtcgaaact
    24661 cctgaccggg gaatgcgacg ttctggcctg gcctgctgcc agccagctat ccattttgcg
    24721 tgacgacccg cgcttgcgtt taacgctgcg tcctgggatg aacgtcgcct atctggcatt
    24781 taacaccgcc aaaccgccgc taaataatcc cgctgtccgc catgcactgg cactggcgat
    24841 taataaccag cgcctgatgc aatccatcta ttatggtacg gctgaaacgg cggcctctat
    24901 tttaccgcgc gcctcgtggg cctatgacaa cgaggctaaa attactgaat acaatccggc
    24961 gaaatcgcgc gaacagttga aggcgttggg gctggaaaac ttaacgctga aactgtgggt
    25021 gcccacacgt tcgcaggcgt ggaaccccag tccactgaaa actgccgaac tgattcaggc
    25081 ggatatggcg caggttggcg taaaagtggt gattgtgccg gtagaaggtc gctttcagga
    25141 ggcgcggttg atggatatga gccatgatct gacattatcc ggttgggcga cggacagtaa
    25201 cgacccggac agtttcttcc gtcctttact gagctgcgcg gcaattcatt cacagaccaa
    25261 cctcgcccac tggtgcgatc cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca
    25321 gcagctggcg gcgcgtattg aagcctatga cgaagcgcag agtattctgg cgcaggaatt
    25381 acctattctg ccgctggcgt cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg
    25441 tctggtactt agcccgtttg gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga
    25501 tgaggtgaaa aaaccatgat tatcttcacc ttacgccgca ttttgttatt gattgtcacc
    25561 ttgttcctgc tgaccttcgt tggcttcagc ttgagctatt tcacccctca tgcgccgtta
    25621 caaggcgcgt cactgtggaa tgcctgggtg ttctggttta acggcctgat ccactgggat
    25681 tttggtgttt ccagcattaa cggtcaaccg attgccgaac agttaaaaga ggtcttcccg
    25741 gcgacgatgg aactgtgcat cctcgccttc ggttttgcgc tgattgttgg catccccgtg
    25801 gggatgattg ccgggattac gcgccataag tggcaggata acttgatcaa cgccatcgcc
    25861 ttactgggct tttcaatccc ggtgttctgg ctggcgcttc tgttgacgct gttttgttca
    25921 ctcacgctag gttggttgcc cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg
    25981 attaccggtt ttgcgttgat tgatgcctgg ctttcggact caccatggcg ggatgaaatg
    26041 atcatgagcg caatccgcca tatgatattg cccgtgatca ccctgtcggt tgcgccaaca
    26101 actgaagtga tccgcctgat gcgtatcagc actatcgaag tgtatgacca gaactatgta
    26161 aaagcggcgg cgacccgcgg tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat
    26221 aacgcgttac cgccggttat tcctcgcctg ggcttacagt tttccaccat gctgacgctg
    26281 gcgatgatca ccgaaatggt ttttagctgg ccgggtttag ggcgctggtt aattaacgcc
    26341 atccgccagc aggattacgc agccatttcc gccggagtga tggtgtgtgg ctcactggtc
    26401 attattgtta acgtgatttc tgatattttg ggtgccatgg ctaaccctct gaaacataag
    26461 gaatggtatg ccttacgata gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac
    26521 cgcctggcgc aaattttata gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg
    26581 gctggctgta ctgtgtattt ttggcggctg gtttgcgcct tacggcatcg accagcaatt
    26641 tctcggttat caattattgc cgccatcatg gtcgcgctat ggcgaagttt ctttcttcct
    26701 ggggactgac gacctcggac gcgatgtttt aagccgttta ctgagcggag ctgcgccaac
    26761 cgtgggtggc gcatttgtgg tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac
    26821 cttcgccggg gcgacacacg gcctccgctc agcggtgctt aaccatattc tggatacctt
    26881 actggcgatc ccttcgctgc tgttggcaat tatcgttgta gcgtttgccg gaccgagttt
    26941 gtcccacgct atgtttgctg tctggctggc attgctgccg cgtatggtac gttcgattta
    27001 cagcatggtg catgacgaac tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc
    27061 atcaacgctg aatattctct ggtttgctgt gatgccaaac atcaccgccg ggctggtcac
    27121 cgagatcacc cgcgcactgt cgatggcaat tctcgatatc gccgcgctgg gctttctcga
    27181 tctcggcgca caactcccct cgcctgaatg gggagcgatg ctcggtgatg cgctggaact
    27241 gatttatgtc gccccgtgga ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt
    27301 gttggttaac ctccttggcg atggcgttcg tcgtgcgatt attgcggggg tggaataatg
    27361 ccattactcg atattcgtaa cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa
    27421 gccgtcgacc gggtaagtat gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa
    27481 tccggttccg gcaagagttt gattgcgaaa gcgatttgtg gggtgaataa agataactgg
    27541 cgtgttactg ctgaccgtat gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc
    27601 gaacggcgca aactggttgg ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt
    27661 cttgaccctt cagaacgtgt gggtcgccag ttgatgcaaa acatcccagc ctggacctat
    27721 aaaggccgtt ggtggcagcg ttttggctgg cgcaaacgcc gtgccattga actgctgcac
    27781 cgcgtgggga ttaaagatca caaagatgcg atgcgcagtt ttccctatga gttgaccgaa
    27841 ggtgaatgtc agaaagtgat gatagccatt gcactggcga atcaaccgcg tctgctgatt
    27901 gctgacgaac cgaccaactc aatggagcca acaacccagg cgcaaatctt tcgcctgctg
    27961 acgcgtctca accaaaacag caataccact attttgctta tcagccatga cttacaaatg
    28021 cttagccaat gggcggataa aattaacgtg ctttactgcg gtcaaacagt ggaaaccgcg
    28081 ccgagtaagg agttggtgac gatgccacat catccttata cccaggcgct gatccgcgcg
    28141 ataccagact tcggcagcgc gatgccgcat aaaagtcgcc tcaatacgct gcccggcgct
    28201 atcccactgc tggaacagtt accgattggt tgtcgtctgg ggccacgttg cccgtatgca
    28261 caacgagaat gcattgtgac gccacgtttg acgggggcga aaaatcatct ctatgcctgt
    28321 catttcccgc tgaacatgga gaaagagtga gatgatcgaa acgctgcttg aagtgcgtaa
    28381 tttaagtaaa acgttccgct accggaccgg ctggtttcgt cgtcagaccg tagaagcggt
    28441 aaaacccttg agctttacgc tacgtgaagg ccagacactg gcgattattg gcgagaatgg
    28501 ttcgggtaaa tccacgctgg caaaaatgct ggcgggaatg atagagccta ccagcggcga
    28561 gttattgatt gacgatcatc cactgcattt tggcgattat tccttccgca gtcagcgtat
    28621 tcgcatgatt tttcaggatc cttcgacctc gttgaatccc cgtcaacgta tctcgcaaat
    28681 tctggatttt ccactgcgcc tgaacaccga cctggaaccg gaacagcgtc gcaaacagat
    28741 tattgaaacg atgcgtatgg ttgggctgct accggatcac gtcagttact atccgcatat
    28801 gttggcaccc ggacaaaaac agcgtctggg tctggcgcgc gcgctaatat tgcgcccaaa
    28861 agtcattatt gccgatgaag ccctcgcttc actggatatg tcgatgcgtt cgcagttgat
    28921 taatctgatg ctggagttac aggaaaaaca gggcatttcg tatatttatg ttacccagca
    28981 tatcggaatg atgaagcaca ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt
    29041 cgagcgaggc agcaccgcgg atgtgctggc atcgccgctg catgaactca ccaaacggct
    29101 gattgccggg cactttggcg aagcattgac agcggatgcg tggcgtaaag atcgctaatt
    29161 ttctgaatcc tccttccaga ttatccacct cattctgaca agagattaaa tcttgtcaga
    29221 atgaggtgat ggataaaata aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc
    29281 aggaacgaaa gctctggcga tatcttcgca gccgacgttt tggtgatttc aaatttcgcc
    29341 gtcaacatcc ggtggggagc tacattctcg attttgcttg ctgctcggcg cgcgtagtcg
    29401 ttgagctgga tggtgggcag catgatttgg cagttgccta tgataccagg cgcactagct
    29461 ggcttgagtc gcagggctgg accgtgctgc gtttctggaa taacgagatt gattgtaatg
    29521 aggaggcggt gccggagatt attctgcagg aactgaaccg ccgatcaccc tcaccctgaa
    29581 agggcgaggg ggccgaccga gccgaatagc tgttgtggtg aaaacatgga gacagtgctg
    29641 gagaatattc ggcaaggtct gaaccatccc agccatcgcc ataaaagggt taggggctgt
    29701 atgagcctgt ttgttgctgg ggtaacaata tttgcacaat atggtcccct cgcccctctg
    29761 gggagagggt tagggtaagg ggaaaagcgc ccaccctgcc gcagcctgct ccggtcggac
    29821 ctggcaacta tagctactca cagccaggtt gattataata accgtttatc tgttcgtact
    29881 gtttactaaa acgacgaatc gcctgatttt caggcacaac aagcatcaac aataaggatt
    29941 aaagctatgg gttttctttc cggtaagcgc attctggtaa ccggtgttgc cagcaaacta
    30001 tccatcgcct acggtatcgc tcaggcgatg caccgcgaag gagctgaact ggcattcacc
    30061 taccagaacg acaaactgaa aggccgcgta gaagaatttg ccgctcaatt gggttctgac
    30121 atcgttctgc agtgcgatgt tgcagaagat gccagcatcg acaccatgtt cgctgaactg
    30181 gggaaagttt ggccgaaatt tgacggtttc gtacactcta ttggttttgc acctggcgat
    30241 cagctggatg gtgactatgt taacgccgtt acccgtgaag gcttcaaaat tgcccacgac
    30301 atcagctcct acagcttcgt tgcaatggca aaagcttgcc gctccatgct gaatccgggt
    30361 tctgccctgc tgaccctttc ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt
    30421 atgggtctgg caaaagcgtc tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt
    30481 ccggaaggtg tgcgtgttaa cgccatctct gctggtccga tccgtactct ggcggcttcc
    30541 ggtatcaaag acttccgcaa aatgctggct cattgcgaag ccgttacccc gattcgccgt
    30601 accgttacta ttgaagatgt gggtaactcc gcggcattcc tgtgctccga tctctctgcc
    30661 ggtatctccg gtgaagtggt ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa
    30721 ctcgaattga aataatcatt ctgttggtaa atattgggcg gcgttctgcc gcccgttatc
    30781 tctgttatac ctttctgata tttgttatcg ccgatccgtc tttctcccct tcccgccttg
    30841 cgtcaggata acgatttcct ttacgaccaa ggagcgccca tggaacaacg ccacatcacc
    30901 ggcaaaagcc actggtatca tgaaacgcaa tccagtactg cggagtatga cgttctgcct
    30961 ctggtcccgg aagccgcaaa ggtcagcgat ccctttctgc tcgacgtgat ccttgatgaa
    31021 gaaacgctgg ctcccttcct ttcatggctg gtccctgcgc gcgttcttgc agtggaattg
    31081 ttccctgacc agcttaccgt gacccgttca cagaccttca ccgcttatga acgcttgtct
    31141 acggccctga cggttgctca ggtttgcggc gtccagcggt tatgtaacta ctattcggcg
    31201 cgacttacgc cgctccccgg gcctgattcc tccagggaaa gcaatcatag gttggcacaa
    31261 atcacgcaat atgcccgcca actggctagc tcgccttcta ttatcgacaa ccgatcgcgc
    31321 cagcatctga atgacgtcgg tcttactgcc tgggactgtg tgatcattaa ccaaatcatt
    31381 ggttttattg gctttcaggc gcggaccatt gcgacatttc aggcttatct cgggcacccg
    31441 gtacgctggt tacccggtct ggaaatacaa aactacgccg acgcgtcact gtttgctgat
    31501 gaatcaatac gctggcgaag cagctatgag gtggaaaaac tacctgaaga gtacacaaaa
    31561 agttcaacag cagaactttg ccaactggcc gaaacactct ctctccaccc tatttcactt
    31621 tcccttctcg aaaggttgtt aaacagtaca cgggttaata cacagccgga taataagctt
    31681 gcggcgttgt tatgcgcgcg gataaatggc agtcctgctt gttttgccgc ctgtatggat
    31741 tcatcaaatg aatataaaaa atcagccccc ttctgcgcaa gggcgaaaat gaaattaacc
    31801 aatgggctga ccgtcattct gttgagcacg ctaccgttca ggcgatacaa tggctgaccc
    31861 gagcacccga tcgctttagc gccgcccagt tcagcccttt actcggacac gaaaaatcat
    31921 caacgcagat tattaatctg ctggtatgga gcgggctgtg tggctggata aatcgcttaa
    31981 aaatcgcgtt gggtgagaca tattaacctt gccgcgtcag acagattcgc gtaaaactgt
    32041 cagccgctct aatggccacc aaaatagaca attatgtttc aggacaaccc gctgctagcg
    32101 cagcttaaac agcaactgca ttcccagacg ccacgcgctg aaggggtggt aaaagccaca
    32161 gaaaaaggct ttggcttcct ggaagtcgac gcgaaaaaaa gttatttcat tccgccgccg
    32221 cagatgaaaa aagtcatgca tggcgaccga attatcgcgg tgatccacag tgaaaaagaa
    32281 cgtgaatccg cagagccaga agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa
    32341 gttcagggca aaaatgaccg tctggccatc gttcctgatc atccactctt aaaagacgcc
    32401 attccttgcc gcgcagcccg tggcctgaac cacgagttta aagaaggcga ctgggcggtt
    32461 gccgaaatgc gccgtcatcc gctgaaaggc gatcgttctt tctatgctga actgacacaa
    32521 tacatcactt ttggtgacga ccactttgta ccgtggtggg ttacccttgc gcgccataat
    32581 ctggaaaaag aagcaccaga cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt
    32641 gaagatctga ccgcgctgga ttttgtcacc atcgacagtg ccagcacaga agatatggat
    32701 gacgcccttt tcgctaaggc gttgccggat gacaaacttc agctgattgt ggcgattgcc
    32761 gatccaaccg cgtggattgc tgaaggcagc aagctggaca aagccgcgaa aattcgcgca
    32821 ttcaccaact atctgcctgg cttcaacatc cctatgcttc ctcgcgagct ttctgacgat
    32881 ctctgctcac tgcgcgccaa tgaagtccgc ccggtactgg catgccgcat gacgctctcc
    32941 gctgatggca ccattgaaga taatatcgaa ttctttgccg ccaccatcga atccaaagcg
    33001 aagctggtgt atgaccaggt ttctgactgg ctggaaaata ccggtgactg gcagcctgaa
    33061 agtgaagcaa ttgccgaaca agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag
    33121 tggcgtcata accacgcact ggtgtttaaa gatcgcccgg attaccgctt tattctcggt
    33181 gaaaaaggtg aagtgctgga tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc
    33241 gaagaagcga tgattgccgc taacatttgt gcggcccgcg ttctgcgcga taagctcggt
    33301 tttggcatct ataacgtgca tatgggcttt gatccggcga atgccgacgc gctggcagcg
    33361 ttgctgaaaa cgcacggtct gcatgtcgat gccgaagaag tgctcacgct ggacggtttc
    33421 tgcaaactgc gtcgtgaact ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt
    33481 cgcttccagt catttgctga aattagcact gaacccggtc ctcactttgg cctcggtctg
    33541 gaagcatacg ccacctggac ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt
    33601 ctgctgaaag cggttatcaa aggcgaaact gcgacgcgtc cgcaggatga aatcactgtc
    33661 caaatggccg aacgtcgccg tctcaaccgg atggcagaac gtgatgttgg cgactggtta
    33721 tacgcacgct tcctgaaaga taaagccggg accggcaccc gtttcgcggc ggaaattgtc
    33781 gatatcagcc gtggcggcat gcgtgtgcgt ttggttgata acggcgctat cgcctttatt
    33841 ccggcacctt tcttacacgc tgtgcgcgat gaactggttt gcagccagga aaacggcact
    33901 gtacaaatta aaggtgaaac ggtttacaaa gtaactgacg ttattgacgt caccattgcc
    33961 gaagtccgca tggaaacccg cggcattatt gcgcgcccgg tcgcgtaatc tcctttcacg
    34021 gcccattcct catggatggg ccgtttattt ccccgctcta ccttcgttat atccaggcag
    34081 attttttaac tgactttcgt ttgaaaactg gcgtttttcc aaaactggat tacaaataaa
    34141 tatataaaaa aacaatgcgt ttagccgccg ttaatttcat gtaagtcatg ttgttccttt
    34201 tatttaaccg cggactccgc agttaaccgg aggatatgca tcatgaaaac cgttagggag
    34261 tccacaacgt tgtacaactt tctcggatcg cacaatccat actggcggtt gacggaaagc
    34321 agcgatgttt tgcgcttttt taccaccgaa accacagaac ctgatcgtac tttgcagtta
    34381 tctgccgaac aggctgctcg catcagggaa atgacggtca tcacctccag cctgatgatg
    34441 agtctgaccg tcgatgagag cgatctttct gtgcatctgg taggacgaaa aatcaataaa
    34501 cgggaatggg ctggcaacgc gtctgcatgg catgacacac cggcagttgc tcgtgattta
    34561 tcacacgggc tttcctttgc tgagcaggta gtttctgaag cacattccgc aatagtgatt
    34621 ctcgacagcc gggggaatat ccaacgcttc aatcggttat gtgaagatta cactgggttg
    34681 aaagaacacg acgtcattgg ccaaagcgtg tttaaactgt ttatgagccg tcgtgaagct
    34741 gcggcatcca ggcgcaataa ccgtgtattt tttcgaagcg gcaatgcata tgaagtcgaa
    34801 ctgtggatac caacacgtaa aggccagcgg ctgtttctgt ttcgcaataa aataaatttg
    34861 tccacagcgg cagtggcaaa aacgagattt ttttaatctg ttccggtacc gacattaccg
    34921 aagagcgccg cgctcaggag cgactgcgta ttctggcaaa taccgacagt atcaccggac
    34981 tgccgaatcg taacgcaatg caggagttaa tcgatcacgc tattaatcag gctgataaca
    35041 ataaagttgg ggttgtgtat cttgatttgg ataattttaa aaaggtcaac gacgcctatg
    35101 ggcatttgtt tggtgaccag ttattacgcg acgtgtcatt ggctatttta agctgtctcg
    35161 aacatgacca ggtgttggcg cgtccaggtg gcgatgagtt tctggtactg gcatccaata
    35221 cctcacaaag cgcgctggaa gcaatggcat cacgaatttt gacccgctta cgcctcccct
    35281 ttcgtattgg tttaattgaa gtttatacca gctgttcagt aggtatttcg ctctctcccg
    35341 aacatggttc ggacagcgcg actattattc gtcacgccga cacagcaatg tacacagcga
    35401 aggaaggcgg acgaggacaa ttttgcgttt ttactccaga aatgaatcag cgggtatttg
    35461 aatatctctg gctggatacc aacttgcgta aagcactgga aaacgatcag ttggttattc
    35521 actatcaacc gaaaatcacc tggcgtggcg aagtgcgcag tctggaagca ctagtacgtt
    35581 ggcagtcacc tgaacgtggg ttgattccac cgttggactt catttcctac gccgaagagt
    35641 cagggctaat tgtgccttta ggccgttggg tgattctcga tgtcgtacgc caggtggcaa
    35701 agtggcggga taaaggcata aacctgcgag tggcggtaaa tatttctgca cgtcagctcg
    35761 ccgatcaaac cattttcacc gccctgaaac aggttctcca ggaactcaat tttgaatact
    35821 gccctataga tgttgaactg acagagagtt gtctgattga gaatgatgaa ctggcactgt
    35881 ctgttattca acaatttagc caactaggtg cgcaagtgca tctggatgat tttggtaccg
    35941 gctactcttc actttcgcaa ctggcgcgct ttccgatcga tgccatcaaa cttgaccagg
    36001 tttttgttcg agatattcac aaacaacctg tctcgcagtc actggtccgg gcgatcgtcg
    36061 ctgtggccca ggcattgaat cttcaggtga tcgccgaagg tgtagagagt gcaaaggaag
    36121 atgctttttt aaccaagaac gggatcaatg agcggcaagg atttttgttt gccaaaccga
    36181 tgcccgccgt cgccttcgaa cgctggtata aacgctatct gaagcgcgca taagtagatg
    36241 aatatatata cttagcgcta tatcccgtca ttatatatcg tattcctgga tgataattag
    36301 ccgttgctgt taaacacttc tcttttagcg gcaatttttg cattattcat tttccacttt
    36361 tgctataagg atgtccttga tgtctgaatt tgatgcccag cgcgttgccg aacgtattga
    36421 tattgtgtta gatatccttg tggcaggcga ttaccactct gcgatccata atctcgagat
    36481 acttaaagca gaactcttgc gccaggttgc tgaatctacg ccagacatcc cgaaggcacc
    36541 gtgggagatt taacaatcgt gccttcaggt tttcctattt gaggatgccc ggcgcgtcag
    36601 tattttttta tttagtatta taacgttata agaattacag cgatgaattc ccgacaacaa
    36661 actattctac agatggtcat tgaccagggt caggttagcg taaccgatct ggcaaaagcc
    36721 actggagttt ctgaagttac cattcgccag gatctcaaca ccctcgaaaa actgagttac
    36781 ctccgccgtg cacatggctt tgcagtttcg cttgatagtg atgacgtcga aacccgtatg
    36841 atgagcaact atacgctgaa gcgtgaactc gccgagtttg ccgcgtcact ggttcaaccg
    36901 ggcgaaacca tctttatcga aaatggcagc agcaatgccc tgctcgcccg gactctgggg
    36961 gagcagaaga aaaatgtcac tatcatcacg gtcagcagct acatcgcgca tttgctgaaa
    37021 gacgcgcctt gtgaagtcat tttgctcggt ggcgtgtacc agaaaaaaag cgaaagtatg
    37081 gttggcccgt tgacacgcca gtgcatccaa caggtgcatt tcagcaaagc atttattggt
    37141 attgatggct ggcaacctga aactggattc accggtcgcg atatgatgcg taccgatgtg
    37201 gtcaatgccg tgctggaaaa agagtgcgaa gcgatagtcc tgactgacag ctcgaaattt
    37261 ggtgctgtac attcatactc catcggtccc gttgagcgat tcaatcgcgt gattaccgat
    37321 tcgaaaatac gcgccagcga tctgatgcat cttgagcaca gtaaactcac cgttcacgtc
    37381 gttgacattt aaccatttcc cttctctacg gatgatttgc agtttggcaa atcatccgct
    37441 ctaagatgat tcctggttga taattaagac tatttacctg ttattaacac gcttaagata
    37501 taaaattatt atcagcgata taacaggaag tcattatcac ctgcgtgata taaccctgcg
    37561 cgcgagcaga tttcacggaa taatttcacc aggcttattc ttagctatta tagttataga
    37621 gagcttactt ccgtgaatca taaattcagg agagagtatt atgtttgtaa cgagcaaaaa
    37681 aatgaccgcg gctgttctgg caattacttt ggcaatgtct ctgagtgcct gttctaactg
    37741 gtctaaacgg gaccgcaaca ccgcaatcgg cgcgggtgca ggggcattag gcggtgcagt
    37801 actgaccgat ggcagtacgt tgggtacatt aggtggtgca gctgtcggtg gtgttattgg
    37861 tcaccaggtc ggtaaataat cgtaaataaa tgtcgattat cgactcgcaa ttataatacg
    37921 cttgattctg ttcagtacga aataactgaa taatacgcac agtaaaatat tcgaaagcca
    37981 cggatatatc cgtggctttt tatgttaacc gcctgcgagt tttaccttca tccctttcgc
    38041 ttcaagtagt gattttaata aatcacgctt atcgccctgg atttcaataa ctccatcttt
    38101 aactgctccg ccgcagccgc attttttctt cagttccgct gcaagttttg tcagttcggc
    38161 atcatcgaga tcgacaccgg taatcaggca aacgccctta cctttacgtc cactggtctg
    38221 acgctgaata cgcaccacac cgtcgccttt agggcgtaca ggggccgctt tgggctcatc
    38281 aatacgtccg gtttccgttg agtagaccag acggctgttg gaatcactca tcatgcactc
    38341 cgctgtaaag aggcgttgat cgctttcagc gtctgcgctg gatctaccga ttgcgttacc
    38401 gggcgaccaa tcaccatata atcaacacca gccgacaacg cctgttctgg cgtcataatg
    38461 cggcgctggt caccagcttc actcccctgc ggacgaatgc ccggcgtaac cagtttgaac
    38521 tcctgaccga atacctgttt aaagcgcaca gcttcctgag cagaacacac cacaccatca
    38581 aggccacatt tttgcgtcag tgccgccaga cgttctgcat aatctgcagg tgacagtgtc
    38641 atgccaagat cgaccaggtc gctggcttcc atgctggtca acactgtcac agcaatcaaa
    38701 agcggtgcat ctttgccaaa cggaaccagt gcctcacgcg ctgcggtcat catacgcgcc
    38761 ccaccagagg catgaacatt caccatccac acgcctaagt cagctgcagc agcgacagcg
    38821 tgcgctgcag tgttaggaat atcgtggaat ttcaggtcaa gaaagatatc aaaaccacgc
    38881 tgttgaagtt cgcgcacaaa ctgtggccca aacaatgtaa acatctcttt gccgaccttc
    38941 agacgacaat cgcgtgggtc gatcttgtcg acaaaggaca gcgcgtcatc acgattatga
    39001 taatcaaggg caacaaccac aggagaattc gtaacagcgc gggaagaaga tgaagcagtt
    39061 aacgtcatga ccagaccttc ttgatgatgg gcaccggaag tggcgcgaaa caggtaaacg
    39121 gcgagcattc tacccgtgga aataaaaaat taacagttgc gatttttccc ctggcaggct
    39181 ttttctgctc cgtcgttgcc ggagcacaat ggaacatcaa taactagtat gttgtaacta
    39241 aagtcggatt tttttaaaaa ttacaggcca tcaagaccgc gaatcggttt aatggttgac
    39301 caggcccgac aagacggaca atgccagtag agggtgtatg cggtaaaacc acatttctgg
    39361 cagcgataac gaggcttact acgtaccttt tcgccaacca tgtcacgcag caccatcagg
    39421 ctctctttgg cacgcccttc ttccgcttca tttaagtggt aatccattaa cttatggaac
    39481 acacgcatgg tcggatgacg ctgaagctgg cgcgtaatat agacctgtgc ggcctcacta
    39541 ccgtcgcgcg cttcgatgat atcagcaagc atcaattcag catcggcacc ggtgttctct
    39601 tccaccgcgc gttgcaggaa ttctgcccat tcggcagttt tacccaactg ctgatagcag
    39661 gtttgcaaca tttccagcgt ttcgctgacc agttctctgt cctgggatat cacgcgttgc
    39721 aaactttcga cggctttggc gtattctcct ttcgccataa acacgcgtcc catcatgatg
    39781 gatacgcggg cgctgttttt atctgccgcc gccccttttt ttagcaaggt catggcacga
    39841 tcgagatcgt cgctggccat atgctgcagg gctaactcac agtagaaatg ggcaatttcg
    39901 acgcgctgtt tatctttacc cagcttcacc aggcgttcgg caacatcaat tgctttctgc
    39961 cactcgctgg tagcctggta gatttgtagc aactgttgca gcgcgccaat gcggaagtca
    40021 gtttcatcgg tcagctgatt gaacatgtct tccgcgcggt cgtataaccc ggcggccatg
    40081 taatcacgcc ccagttgttg aatcgccaac agacgctgtt cataggtcag tgaggcgctt
    40141 tccattaggg tctgatggat gcgaatagcg cgatcaactt cgccacgcga acggaacagg
    40201 tttccgagcg taaggtgggc ttcaacggtg cctgtatcct ctttaagcat atcgagaaac
    40261 aggtctaccg ctttatcctg ttgattacta agcaggaagt taacccccgc tacgtaatca
    40321 cgcgacaagc ggttggcttc atcttgcttg ttttgttgcg cacttctgcg gcccatatac
    40381 cagccatagg cagcggctac aggcaaaagc agaaacaaca actccagcat agaaagttat
    40441 tccttcgccg ctgacgagtg cggcactaca gccacgtcag tcgcgggtga aagctggttt
    40501 tccagtcgct ttattttacg ttcagcgcgc gccagggaaa cacgaactcg cagccagaac
    40561 aggccacaaa tcaaccaacc gatagcaacc cccgcagcaa acaataccgc cagcaatgtg
    40621 gaaatacggt actccccttg cgctaacaga taattaaacg tcacctgttg atcgttctgc
    40681 gcacccaacg tgaccgaaat cacgaagatc gctaacacca gtaagaaaat gagtaaatat
    40741 ttcacattac ttcccgttat gtggttaaag cgaataaatt atggtcaact taccgcaata
    40801 gccctttaaa gtaccatttt cgcgccgtct gcgaaatggc atccacgcgc attgttgatg
    40861 atttaagggc taaattcgga aaatcaacca gcgttaactt tcttgttctc gttgcgctat
    40921 ttcgcgattt tcttccgcag gtggtgttaa tggcccacaa attcgttgcg caagccacgt
    40981 tgccaccgcc accagcgccc acgaaatcaa cgtagctact accagatcgc gtggccaatg
    41041 catcccgagc agcaggcggc ttcccatgac tcccgttgcc cagaccagca agatagcaat
    41101 ggttaacgtt cgccgacgcg gccacagcaa accaacggcc agcagtgccc aactggcagc
    41161 aaacatcgtg tgaccggaag gaaaggcaaa ccccgtctct ttctgccagt gtgaacgcaa
    41221 atattgtggg atatttttct cttcagccaa ctgttctttc actagatttc cgcgttctgc
    41281 tcgctttaaa gtgtagaact catcaaccgg aatatgatgt gttttttcca gccagataac
    41341 aaaaggtcgt ggttcctgga ctttgtcttt gatccaggat ttaacgcctt gtcccacaat
    41401 gattacggcc gccagaatgg caaataatac aaaggcagcc ttaatgcaaa aacgcagaca
    41461 ccagagaaac cagccgaata aaatcaaatg tgtaatgacg ccccagggct gggtgacagt
    41521 ttcagtaacc caaaaagccg cttttagtag ccaactttgt tctccaggtt gccaacgcca
    41581 gccagaaatc catacggcta ctggcatgac aagcaatagt gcagctccca ctgcggtacg
    41641 tctggcaatc gaacgcatgg cctctccttt ttgataagtc ccacaatcat aactgaaaac
    41701 gccagttcca ggaaaaattg acagatttgt gccattccgt gaacgatcga cgcgtcgtga
    41761 ttaggtgaac cccttctcgt tatggcaaaa taagccaata cagaaccagc attatctgga
    41821 gaatttcatg cagcttaaac gtgtggcaga agccaaactg ccaaccccat ggggcgattt
    41881 cctgatggtg ggatttgaag aactggcaac cggacacgat catgtcgcgc tagtctatgg
    41941 cgatatttcc gggcataccc cggtacttgc gcgcgtccat tccgaatgtc tgaccggtga
    42001 cgccctgttc agcttgcgct gcgattgtgg cttccagctc gaagcggcat tgacgcaaat
    42061 tgccgaggaa ggccgtggta ttttgctgta tcaccgtcag gaaggtcgta acattggtct
    42121 gctgaataaa atccgcgctt acgcactgca ggatcaaggt tacgataccg tagaggctaa
    42181 ccatcagtta ggcttcgccg ctgatgagcg cgacttcact ctttgcgctg atatgttcaa
    42241 actccttggc gtcaatgaag tccgcttgtt aaccaataac ccgaaaaaag tcgaaattct
    42301 gaccgaagca gggattaata ttgttgaacg cgtaccattg attgtaggtc gtaaccccaa
    42361 taacgaacat tatctcgata ccaaagccga gaaaatgggt catttgctga acaaataacc
    42421 ctctcgcatt gtggaattaa tttgcttgcc ggaagcaaaa taaccggcaa gcaaatattt
    42481 gttacttcag catattacga atgacataat gcaaaatgcc gtcgttctgg tagtaggtca
    42541 actccgtcgc ggtgtcgata cgacaacggc agggtacgac ctcctggcta ccatccgcgc
    42601 gcgtaagcgt caccggaacc gtcgcaccgg gttgtagatt ttgcagatcg ccaatatcaa
    42661 tcttctcttc cccggttagt cctaacgttt tacgcgttac gccttgcgga aattccagcg
    42721 gcaggatgcc catgccaatt aaattcgaac ggtgaattcg ttcaaacgat tcggcaatca
    42781 ccacacgaat tccaagcaga cgaggacctt ttgccgccca gtcacggctt gagcctgaac
    42841 catactcttt cccggcaatc accgccagcg gcgtttgttc ctgcttatag cgcatcgcag
    42901 catcataaat agagactacg tcgctgtcag gtaaatgccg cgtcatcccc ccttcaacgc
    42961 caggcaccat ttcattacgg atgcgaatat tggcgaaggt gccgcgcatc atcacttcat
    43021 ggttaccacg ccgcgaaccg taggagttaa agtcttttcg ctcaacaccc cgaccttgta
    43081 gatatcgacc cgctgggctg tcgggcttaa tactgcccgc cggagagata tggtcagtgg
    43141 tgactgaatc ccccagcatt gcgaggatcc gcgcaccgtg aatatcttcc actggtgctg
    43201 gtgttgcctg catttcatca aagaaaggcg ataagcgaat ataggttgag tcctcctgcc
    43261 aaccgtaggt atcggatcgt gtgacgttaa tttccttcca ctctgctgtg ccttcaaaaa
    43321 cttctgcgta ctctttgcgg aacatttctg tggagacttg ttctaccgca cgggcaattt
    43381 cttgtgccga tggccagata tctttcagat aaaccggatc gcctttacga tcatgaccaa
    43441 taggctcaga agccaggttg atattcatat ttcccgccag cgcataggca accaccagcg
    43501 gcggcgaggc cagccagttg gttttaacca gcggatggat acggccttca aagttacggt
    43561 tgccggacag cactgcaccg acggttaaat cgcctttttt gattgccgtt tcgataggat
    43621 cgggcagcgg cccagagtta ccaatacagg tagtacaacc gtatcccaca aggttaaacc
    43681 ccagttcgtc gagaaacggt gtcagtttcg cttttgccag atagtcagaa acaactttcg
    43741 agcctggtgc cagcgacgct ttgacccacg gttgccgctt gaggccaaga attacggctt
    43801 ttttcgccag caagcctgca gccatcagca cacttgggtt agaggtgttg gtgcacgagg
    43861 ttatcgcagc aatgaccaca gcgccatcag gtaactgata ctgatgtccg ttcataacat
    43921 aatcgaccgg ctgacgatct ttatgcgtgg cattcacttc cagttcgtta ctggcggcaa
    43981 atacttttgg tacatcgggc agtgcaacgc gatcctgtgg gcgttttggc cctgccaggc
    44041 tcgcttcaac gtcattcata tccagttcta acgtactggt aaaaatcggt tcatcgcccg
    44101 ggttacgcca catgccctgc gctttggcat atttttcgac caactcaacc tgatcttcgc
    44161 tgcgcccgct taaacgcatg taatcgaggg ttacagcatc gattgggaag aagccacagg
    44221 tggcaccata ttctggcgac atattggcaa tggtggcgcg atccgccagc ggtagtgaat
    44281 ccagaccatc accataaaat tcgacgaatt tctccaccac gccatgtttg cgcagcattt
    44341 gggtaacagt gagaaccagg tctgtggcgg taataccttc acgtaattta ccggtaagtt
    44401 taaagcccac tacgtccggg ataagcatgg aaaccggctg gcctaacatt gcggcttctg
    44461 cttcgatccc acccacgccc caccccagca cgccaaggcc gttgatcata gtggtgtgcg
    44521 agtcagtacc aacgagtgta tccggataag caatccattc accgtcctgc aattcactcc
    44581 acactgcttt gccgagatat tcgaggttaa cctgatggca aatgcctgtg cctggcggca
    44641 cgacgctaaa ccgactgaac gcttgctttc cccatttcag gaacacataa cgttcgtggt
    44701 tgcgctccat ttccaggcgt acgttttctt caaatgcctc atcatcacca aaacgatcga
    44761 cggtcaccga gtggtcaatg accaggtcga ccggtgagag cgggttaacc tttgcagtat
    44821 cgccgccgag gcgtttaacc gcttcgcgca ttgccgccag atcaacaacg gcaggtacgc
    44881 cggtaaagtc ctgcatcaac acccttgccg ggcggtaggc aatttcacgg tcagcatggg
    44941 catttttcag ccatcctgcc agcgcgtgga tatcctcttc ggtaaccgag ttaccatcct
    45001 gccagcgcag caggttttcg agcaaaactt tgagtgactt gggtagacgg gtgatatcgc
    45061 ccagtgattt agcagcaagc ggcaggctgt agtagtggta agttttatct ttggcctgca
    45121 acgtgtcctt actggcttct cgtagggttg acgacatagc tcctccttaa tgacagggtt
    45181 gcgaagccct gattctcttc agggtgaata ttaaagataa cacaaacatc gcgtaacgat
    45241 ttgataacaa cccaaattga taaaagagga gaaaccttgt ggcaaagcaa atgacaaccc
    45301 cgccgcagcg gggtcaagga tcagtgagta atgatccaga ggagttcggc ccagaagagc
    45361 atcgacagag aaaacgcgcc catccaggac caatatttca tatttgtgtt gatcataggc
    45421 tacaccctta acggttatta ttttgtgttg agataactca agctgttatt gctgcggtgt
    45481 attaaaagcc ctttaattca ttaaaaatgg gtaataagaa tgatgataat tattgtttcg
    45541 gctgatcatc ttcagcaaac agttctataa aggcttgctg ctgcgatgtt agttgccagt
    45601 cgtctggaat aacgacggaa cattcccgtt tcgtttctgt ttcattgtca ctatgctggc
    45661 ataatggtga cgactccagt tgctcctgac cgaccatagt tatccccaca ctttccaaat
    45721 cagcagtgcg acaaccaccc agaaaagtgc agaaccaaga aaaaccgcca gccaggcttt
    45781 acgttttaat tcaggatcgc ggcggggttc ctgatttcca gaaggcattg ctaacctcat
    45841 ataatcgcca tcacttatca gcaagacgtt gaacgattgg tgtaattaat catacgatga
    45901 gacaaatcct aaag
//
LOCUS       DAJTYZ010000015        43082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000015 DAJTYZ010000000
VERSION     DAJTYZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43082
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.015"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..559)
                     /locus_tag="OO596_000854"
     CDS             complement(<1..559)
                     /locus_tag="OO596_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6716791.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="OO596_000855"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="OO596_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6716792.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="OO596_000856"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="OO596_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6716793.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="OO596_000857"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="OO596_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6716794.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="OO596_000858"
     CDS             complement(4001..6322)
                     /locus_tag="OO596_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6716795.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="OO596_000859"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="OO596_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6716796.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="OO596_000860"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="OO596_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6716797.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="OO596_000861"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="OO596_000861"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6716798.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="OO596_000862"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="OO596_000862"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6716799.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPEIW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..11582
                     /locus_tag="OO596_000863"
     CDS             11268..11582
                     /locus_tag="OO596_000863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6716800.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11595..12968
                     /locus_tag="OO596_000864"
     CDS             11595..12968
                     /locus_tag="OO596_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6716801.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="OO596_000865"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="OO596_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6716802.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="OO596_000866"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="OO596_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6716803.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="OO596_000867"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="OO596_000867"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6716804.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="OO596_000868"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="OO596_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6716805.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="OO596_000869"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="OO596_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6716806.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="OO596_000870"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="OO596_000870"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6716807.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="OO596_000871"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="OO596_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6716808.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="OO596_000872"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="OO596_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="OO596_000873"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="OO596_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6716809.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="OO596_000874"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="OO596_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6716810.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRREQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="OO596_000875"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="OO596_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6716811.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="OO596_000876"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="OO596_000876"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6716812.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="OO596_000877"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="OO596_000877"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6716813.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="OO596_000878"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="OO596_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6716814.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="OO596_000879"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="OO596_000879"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6716815.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="OO596_000880"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="OO596_000880"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6716816.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="OO596_000881"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="OO596_000881"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6716817.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="OO596_000882"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="OO596_000882"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6716818.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /locus_tag="OO596_000883"
     CDS             31280..32185
                     /locus_tag="OO596_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716819.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="OO596_000884"
     CDS             32202..32564
                     /locus_tag="OO596_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6716820.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="OO596_000885"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="OO596_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="OO596_000886"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="OO596_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="OO596_000887"
     CDS             35034..35912
                     /locus_tag="OO596_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6716821.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="OO596_000888"
     CDS             35923..36276
                     /locus_tag="OO596_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6716822.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="OO596_000889"
     CDS             36288..37592
                     /locus_tag="OO596_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6716823.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVMRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="OO596_000890"
     CDS             37604..38689
                     /locus_tag="OO596_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6716824.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="OO596_000891"
     CDS             complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="OO596_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6716825.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="OO596_000892"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="OO596_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6716826.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="OO596_000893"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="OO596_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="OO596_000894"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="OO596_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6716827.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..>43082)
                     /gene="frlA"
                     /locus_tag="OO596_000895"
     CDS             complement(42378..>43082)
                     /gene="frlA"
                     /locus_tag="OO596_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR6716828.1"
                     /translation="ASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMP
                     FDKLANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKD
                     NLFFKCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGS
                     IIWCRKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLP
                     AYAFWAKRSRQLNALS"
BASE COUNT         9990 a        10954 c        11818 g        10320 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccagatt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggagcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcatgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cgttcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gc
//
LOCUS       DAJTYZ010000016        42520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000016 DAJTYZ010000000
VERSION     DAJTYZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.016"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            254..2020
                     /gene="adeD"
                     /locus_tag="OO596_000896"
     CDS             254..2020
                     /gene="adeD"
                     /locus_tag="OO596_000896"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6716829.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="OO596_000897"
     CDS             complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="OO596_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6716830.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="OO596_000898"
     CDS             complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="OO596_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6716831.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="OO596_000899"
     CDS             complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="OO596_000899"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6716832.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="OO596_000900"
     CDS             complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="OO596_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6716833.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="OO596_000901"
     CDS             complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="OO596_000901"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6716834.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="OO596_000902"
     CDS             complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="OO596_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6716835.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="OO596_000903"
     CDS             complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="OO596_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6716836.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9841..9930
                     /gene="tisB"
                     /locus_tag="OO596_000904"
     CDS             9841..9930
                     /gene="tisB"
                     /locus_tag="OO596_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6716837.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10210..11394
                     /gene="emrD"
                     /locus_tag="OO596_000905"
     CDS             10210..11394
                     /gene="emrD"
                     /locus_tag="OO596_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6716838.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSSVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11402..11899)
                     /locus_tag="OO596_000906"
     CDS             complement(11402..11899)
                     /locus_tag="OO596_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6716839.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11896..12258)
                     /locus_tag="OO596_000907"
     CDS             complement(11896..12258)
                     /locus_tag="OO596_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6716840.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12248..12595)
                     /locus_tag="OO596_000908"
     CDS             complement(12248..12595)
                     /locus_tag="OO596_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6716841.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12704..13153
                     /locus_tag="OO596_000909"
     CDS             12704..13153
                     /locus_tag="OO596_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716842.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13402..14610)
                     /locus_tag="OO596_000910"
     CDS             complement(13402..14610)
                     /locus_tag="OO596_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6716843.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSLPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRC
                     VCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            14618..15049
                     /gene="tnpA"
                     /locus_tag="OO596_000911"
                     /pseudo
     CDS             14618..15049
                     /gene="tnpA"
                     /locus_tag="OO596_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(15180..16503)
                     /locus_tag="OO596_000912"
                     /pseudo
     CDS             complement(15180..16503)
                     /locus_tag="OO596_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(16503..18119)
                     /locus_tag="OO596_000913"
     CDS             complement(16503..18119)
                     /locus_tag="OO596_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6716844.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            18415..19131
                     /locus_tag="OO596_000914"
     CDS             18415..19131
                     /locus_tag="OO596_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6716845.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(19128..20789)
                     /locus_tag="OO596_000915"
     CDS             complement(19128..20789)
                     /locus_tag="OO596_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6716846.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(20985..21413)
                     /gene="ibpB"
                     /locus_tag="OO596_000916"
     CDS             complement(20985..21413)
                     /gene="ibpB"
                     /locus_tag="OO596_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6716847.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(21525..21938)
                     /gene="ibpA"
                     /locus_tag="OO596_000917"
     CDS             complement(21525..21938)
                     /gene="ibpA"
                     /locus_tag="OO596_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6716848.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(21932..22004)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            22244..22576
                     /locus_tag="OO596_000918"
     CDS             22244..22576
                     /locus_tag="OO596_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6716849.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(22578..23792)
                     /locus_tag="OO596_000919"
     CDS             complement(22578..23792)
                     /locus_tag="OO596_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6716850.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            23893..24957
                     /gene="cbrA"
                     /locus_tag="OO596_000920"
     CDS             23893..24957
                     /gene="cbrA"
                     /locus_tag="OO596_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6716851.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(24954..26291)
                     /locus_tag="OO596_000921"
     CDS             complement(24954..26291)
                     /locus_tag="OO596_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6716852.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(26366..27514)
                     /gene="dgoD"
                     /locus_tag="OO596_000922"
                     /pseudo
     CDS             complement(26366..27514)
                     /gene="dgoD"
                     /locus_tag="OO596_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(27511..28128)
                     /gene="dgoA"
                     /locus_tag="OO596_000923"
     CDS             complement(27511..28128)
                     /gene="dgoA"
                     /locus_tag="OO596_000923"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6716853.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(28112..28990)
                     /gene="dgoK"
                     /locus_tag="OO596_000924"
     CDS             complement(28112..28990)
                     /gene="dgoK"
                     /locus_tag="OO596_000924"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6716854.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(28987..29676)
                     /gene="dgoR"
                     /locus_tag="OO596_000925"
     CDS             complement(28987..29676)
                     /gene="dgoR"
                     /locus_tag="OO596_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6716855.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            29645..30277
                     /locus_tag="OO596_000926"
                     /pseudo
     CDS             29645..30277
                     /locus_tag="OO596_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30323..31135)
                     /gene="yidA"
                     /locus_tag="OO596_000927"
     CDS             complement(30323..31135)
                     /gene="yidA"
                     /locus_tag="OO596_000927"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6716856.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(31250..31648)
                     /locus_tag="OO596_000928"
     CDS             complement(31250..31648)
                     /locus_tag="OO596_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6716857.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(31888..34302)
                     /gene="gyrB"
                     /locus_tag="OO596_000929"
     CDS             complement(31888..34302)
                     /gene="gyrB"
                     /locus_tag="OO596_000929"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6716858.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(34331..35404)
                     /gene="recF"
                     /locus_tag="OO596_000930"
     CDS             complement(34331..35404)
                     /gene="recF"
                     /locus_tag="OO596_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6716859.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(35404..36504)
                     /gene="dnaN"
                     /locus_tag="OO596_000931"
     CDS             complement(35404..36504)
                     /gene="dnaN"
                     /locus_tag="OO596_000931"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6716860.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(36509..37912)
                     /gene="dnaA"
                     /locus_tag="OO596_000932"
     CDS             complement(36509..37912)
                     /gene="dnaA"
                     /locus_tag="OO596_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6716861.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            38209..38289
                     /locus_tag="OO596_000933"
     CDS             38209..38289
                     /locus_tag="OO596_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716862.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            38519..38659
                     /gene="rpmH"
                     /locus_tag="OO596_000934"
     CDS             38519..38659
                     /gene="rpmH"
                     /locus_tag="OO596_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6716863.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            38676..39035
                     /gene="rnpA"
                     /locus_tag="OO596_000935"
     CDS             38676..39035
                     /gene="rnpA"
                     /locus_tag="OO596_000935"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6716864.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            38999..39256
                     /gene="yidD"
                     /locus_tag="OO596_000936"
     CDS             38999..39256
                     /gene="yidD"
                     /locus_tag="OO596_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6716865.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            39259..40905
                     /gene="yidC"
                     /locus_tag="OO596_000937"
     CDS             39259..40905
                     /gene="yidC"
                     /locus_tag="OO596_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6716866.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            41011..42375
                     /gene="mnmE"
                     /locus_tag="OO596_000938"
     CDS             41011..42375
                     /gene="mnmE"
                     /locus_tag="OO596_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6716867.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10165 a        11578 c        10893 g         9884 t
ORIGIN      
        1 tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat
       61 cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta
      121 aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat
      181 attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca
      241 aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta gccgggctga
      301 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
      361 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
      421 acgttacatt gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga
      481 tgctcgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag
      541 catgatgacg ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat
      601 ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg
      661 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct
      721 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      781 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
      841 gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc
      901 gggtttgggt ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga
      961 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
     1021 cgaagggtcc gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag
     1081 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca
     1141 catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
     1201 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
     1261 acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1321 ggtactggtg aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag
     1381 actggcacaa tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag
     1441 cgactttgcc ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1501 cgaattgatt acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga
     1561 tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt
     1621 gcttggcggc tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca
     1681 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1741 tcaggatggc ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt
     1801 gcccattgcc ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc
     1861 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1921 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgt
     1981 cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg
     2041 ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg
     2101 gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc
     2161 ggcgatatca agtgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc
     2221 atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt
     2281 gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac
     2341 accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata
     2401 gatatattcg ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc
     2461 gatgcaggcc accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag
     2521 cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga
     2581 gagtttcagt tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac
     2641 atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc
     2701 aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat
     2761 ctcctcgccg aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc
     2821 gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc
     2881 gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc
     2941 accgccaagg ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg
     3001 cgtccatttg gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct
     3061 taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact
     3121 gaagcccagc atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt
     3181 tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc
     3241 caggccgatc atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg
     3301 cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag
     3361 gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag
     3421 ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct
     3481 gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa
     3541 aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct
     3601 tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg
     3661 atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc
     3721 caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct
     3781 gccgcctctt tgtgggaaca ctctgccgcc gccataccga ttaacatctg tgggccaaag
     3841 acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc
     3901 agccacaggg agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccct
     3961 cggttgccgt taaacaattt gtccgagccc caacccgcta ccagcgcacc gataaatcca
     4021 cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg
     4081 gacatataca aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag
     4141 caaaacgaaa gcagccagat atacggattc agcaacacat atttggtgag gatctcttta
     4201 cgcgtcagcc ctgccccttc ttgttgttga gctatctcca gcgcgtcgtg tcgccattca
     4261 ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt
     4321 cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgtagt
     4381 gcagccgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat
     4441 aatgcccacc aaccaccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac
     4501 accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat
     4561 agcgacgtcg agaagccaaa cagaatgttg ataatgcccg tggcgataag ccctatcccc
     4621 ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgcca
     4681 taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta
     4741 gcaaggattt ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa
     4801 cccagccaga tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca
     4861 tatttatcag ccattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa
     4921 ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg
     4981 tgtaatgtgc cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt
     5041 tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa taaccagcat caaccgttca
     5101 tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttgttc
     5161 agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgattca
     5221 tcgattcgcc attcgagatg gctgacaata ccgcgccctt ccagctccat ttcccgcatc
     5281 agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgaccc
     5341 aacaaacggc gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc
     5401 tgcttcacgc tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata
     5461 gcagtgatgg tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc
     5521 tcttcggttt ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc
     5581 gactggttaa gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt
     5641 tgcaccagca gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc
     5701 agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc
     5761 agcgcgataa tcggcagcac cagacagaat ggcgtaaagc gcgacagttc gtccggcaat
     5821 cccaactgga gccagagact gataacaaac agcagcaagt accagaccag atgtcgcccg
     5881 cgccagttga ttggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg
     5941 agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc
     6001 aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc
     6061 gccgccgtta acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag
     6121 cgcgagatca gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa
     6181 tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat
     6241 acgggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat
     6301 aacagcaccg ccatatcagg gcgctcaacc agatgcaggc tgatactcca caggcaaaac
     6361 catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc
     6421 ttcatcacca gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt
     6481 tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg
     6541 caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg
     6601 ttagcgggtc ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc
     6661 cacccgtggc aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa
     6721 gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg
     6781 agagcataat cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat
     6841 cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg
     6901 cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct
     6961 gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct
     7021 tgtcctggcg ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc
     7081 cttgagcggt tactgaaaaa acaccgcgat cttgttaaac atcgtcggat cggactgatt
     7141 acgctgcact ttcacgacat cttccagctt atcgatttgg cttatcatct gctccagacg
     7201 ctggtcgtca ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca
     7261 aagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac
     7321 gcccggatgg ttgcgaacgg tgagctccag aattacgttg tcatgagttg tgttttgcat
     7381 ggcttattcc cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt
     7441 ttcttcggca tcaatgcgca catggatcag cgccgggcta gggcgattga tgatttcctg
     7501 caatgcagcc tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc
     7561 ggcaatctgc ataaagttga ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta
     7621 gaacagactc tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt
     7681 gacatccagc tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc
     7741 gtcgccggag aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc
     7801 aggcaggcca aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt
     7861 gagcggataa gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc
     7921 attgtcatcg acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca
     7981 cgctttcggg attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc
     8041 tgcacgcggt tgcgcttcca cctgcgggat caattgcgcc agcacgtcat caacatccgc
     8101 ctgaatcgcc acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg
     8161 aataattttg gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg
     8221 cgcaccgagc acaatcaaca aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc
     8281 gtgcatcccc agcataccca gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat
     8341 taaagtcatg gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc
     8401 attgatcaca ccaccaccca gataaagcac cgggcgtttg gcagcgttaa tcattgtagc
     8461 tacgtcacga atgctttctt cgctaaaggc gggtgcagcg gctttttctg ccaccgcggg
     8521 ctgagcttca atctcaaaaa ccgccgtttg tacatcctta ggaatgtcta tccacaccgg
     8581 gccagggcgg cctgattgcg caatgcggaa ggcatcgctc atgacctgcg ggagttcttc
     8641 gatatgtctg accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtcgac
     8701 ttcctggaag gcgtcggtgc cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag
     8761 cgggatggag tccagccgcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc
     8821 gctacaggcc atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa
     8881 gcccgcgccc tgttcatgac gggccagaat atggcggatt tgcgtgcttt ggcttaaggc
     8941 atcgtaaaca ggcaggatag aaccgcccgg aatgcctgtc acaatcttaa tgccctgctg
     9001 ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtca atgttgtgcc
     9061 cgaacttgcc atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg
     9121 aaaaaccccg cccggtttgc gccgacgggg ttttggaatc gtgtgttgtt ccagtcccta
     9181 cggcgcattg ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt
     9241 tggtagtagt tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt
     9301 taacaatatt tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg
     9361 gaaaattgag gggttgatca cgttttgtac tgaattgcag ataacaaaaa accccgccgg
     9421 agcgaggttt cgtcagtcgc ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac
     9481 acagaaagta tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt
     9541 agtgcatagt tgagtatcga tcacagtttg cgttttgtcc aaatattgct gtttatttat
     9601 acagtaaact tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc
     9661 cggcgcacta agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc
     9721 acactgtgtt atgtcaacaa gcacaaagtt tctccttgag ataccgcgtg cacaacagct
     9781 ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt
     9841 atgagcctgg tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg
     9901 cttgatgctg ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg
     9961 ggtaaccgct ccccttttat atttagcgtg cgggttggtg tcggatgcga tgctgacgta
    10021 tcttatccgc cctaccatct ctcccggcaa catttattgc cgcttttgtt tacatattct
    10081 gccgctaaac aactccccat tcctggcgta tatctggcta acattcatca atgtgataga
    10141 ttcctctccc gcatttatgg gaatgcgtag tgacttactc taattatttt tataaaagca
    10201 tccgtgataa tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg
    10261 gccgtcggtc agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat
    10321 ctcaacgtcc gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt
    10381 gtctcacagc tgttttatgg cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc
    10441 gtcggaatgt ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgacg
    10501 gtattgattg ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg
    10561 cgtactttgc cgcgagattt atatgaacgg acacagttgc gccatgctaa cagcctgtta
    10621 aacatgggga ttctcgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg
    10681 atgtggaact ggcgcgcctg ttatctcttt ttgttggttc tttgtgctgg tgtgaccttc
    10741 agtatggccc gctggatgcc ggaaacgcgt ccggtcgatg caccgcgcac gcgcctgctt
    10801 accagttata aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt
    10861 ggcggtctgg ccgggattgc cgcctttgaa gcctgctcca gcgtgctgat gggcgcggtg
    10921 ttagggctga gcagtatgac ggtcagtatt ttgtttattc tgccgattcc agcagcgttt
    10981 tttggcgcat ggtttgccgg acgtcccaat aaacgcttct ccacgttaat gtggcagtcg
    11041 gttatctgct gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg
    11101 aatgtctgga cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt
    11161 cctctggcga ccagcggcgc gatggagccg ttccccttcc tggcgggcac ggctggcgcg
    11221 ctggtcggcg gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg
    11281 ccgcaaaccg gtcagggcag cctagggttg ttgatgacct taatgggatt gttgatcgtg
    11341 ctgtgctggc taccgctggc gacgcggatg tcgcatcagg ggcagcccgt ttaagcgcac
    11401 gtcaccgcag catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca
    11461 tcacccgcat atgcggtgcg gagtaagaat aaaacgcctg atagcccgca caaagcacgc
    11521 tgtctggcgt ctcctgccga gcgtgcgggc acaggcgatg ccagtcgcac gcctggcagt
    11581 cacagcgcgg ttgcgggttt tcgctgccac accatttttg taaggtggcg tcaaaaagct
    11641 caatcgagat acgactgata tctgcccgca cccaacactc gaacagggca attaaaaatt
    11701 tcccccacag ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa
    11761 gatagggaat gagctgtacg gagcggatgc cccgctgttt gaaattgtga taaaaatcgg
    11821 caggttgcag agcatccttg tactctacaa ccagtttatg cctgtcctct tcagcatcaa
    11881 tgacctgact tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt
    11941 tacagcaaac agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac
    12001 tacgtgaaat tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgggtgta
    12061 gtgccagagg atcagcgcca cgatggcgag aatgccaatc gaaatccaga ataacatgcc
    12121 cgcctggtgc cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt
    12181 acgaaaccac gccagtgatg tgcgctccgg ctgcagtcct gggtcggcaa tgcgtctggc
    12241 tttgcggcta tccggcatac aacaccagtc ccataacgat caccgccacg accatcaata
    12301 ttaagctaat gattaataag ctgttggtgt agggcaggtc ctctttcaga cgcatcgctt
    12361 tttcattgcg taaccagcgc aggtagccat acattgctaa cccgccggaa aacagacaca
    12421 acaacaacgc cagcagttca cgaatgacgg gcgtggcgaa atctggcgca agttgatcaa
    12481 gcccaacccc cgccgccaga aatcctagtg cggtacggat ccacgccaga aaagtacgct
    12541 cattagccag cgagaagcgg taatcgggcg cttctccaag gcgggaaatt ttcattgttg
    12601 ctcctttgca atttcactaa ctctctacct ttagcggttt tttttaagaa tttcgtccgc
    12661 ccgtttcctg atatgctttt gccatccgct acaaggaggt cagatgggta tcatagcgca
    12721 aaataaaatc agttcattgg gcatgctgtt cggtgccatc gctttaatga tggggattat
    12781 ccacttttca ttcggccctt tttccgcacc accgccaacg cttgaaagca tcgtggcgga
    12841 caaaacggct gaaattaaaa gaggtcttct cgccggaata aaaggcgaaa aaattaccac
    12901 cgtggagaaa aaagaggacg tagatgtcga taaaatcctc gatcaaagcg gtatcgcact
    12961 ggccattgct gcgctacttt gcgcatttgt tggcggtatg cgtaaggaaa atcgctgggg
    13021 cattcgcggc gcgctggttt ttggcggcgg aacgctggct ttccatacct tgctttttgg
    13081 tatcggcatc gtgtgcagca ttttgctgat attccttatc ttttccttcc tgaccggcgg
    13141 ttcgctggtt taagcagcct gaataatgcc cgccgggtga tcatcagcgg gcattatcgt
    13201 cagaacttct gttttttctc ttccaccttc accccttgtg tcggtaggcc ggtgtcatag
    13261 tcacgtacca ccggcgaata gccatcttgt gggcgtggac gaaatgcccc catccagcgc
    13321 ggccgtgcat ctttacgcca cggacgcagg ctccattgtt gacatcctcc acgccctgaa
    13381 ggacgtggcc ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt
    13441 caacgagcgg cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa
    13501 aatgttacga gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa
    13561 tttactttgt gacaggcgat tttctttcgc tgtatgacca cagcacacgc aacgctggct
    13621 tgtgtacgct ggcggaacag caagcacctg accaccacgc cagagctgct tgtacgcaag
    13681 ctggcggcgc atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc
    13741 ccggacattg cgccccggca ggctgaccgt acccgctgct gactttgaca tgttgctgac
    13801 cttcaaatcc tcaatgacta tcattgcgtg gtttttgctg acggtcgttg tgactttgtg
    13861 aaggtagtcc ctgcggatat ttgcgataca ggaatgcagt cgctgtattt tgcgtttctg
    13921 cttctgccag ttgttgctga atttgacctt gcggcttaac tgtcgctgaa gtctcgccag
    13981 cttcttctgg tttttctgaa aactgtttac aggctcaaag actgtgccat ctgacagcgt
    14041 ggcgagttta gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt
    14101 tgatacttca ctttctgtct gaatactgat gtaccactta ccgcaggact ggctgacagt
    14161 gacatttttc acaacacccg tgacctgacg gctgttccgg tagcgcatcc agcccagttt
    14221 cggcagaaaa atacggctgt tttcctgatc gagcttaacg ccctgcgggt agcggaatac
    14281 atcattctgt ccccgctttt tgaatcgggg aaaagcagcc cgattctgga agaagttttt
    14341 gtaagcccgc tcaaggtctt tcagtgactg ttgcaatggc tgtgacgggg catctttaag
    14401 ccattgcgtt tcagtggcat ttttccactc aaccagccag gaagccattt tcccgtaagg
    14461 gatgtattta ttaccggcct catgattctc attctgacgt gccagagcac gattgaaaac
    14521 gaaacgacat gcgcctgcga agcgcctcat ttcacgctcc tgttgaccac cgggtcttaa
    14581 ctggaattta aatgcttgta gtcgtttcac aggccaaatg atactttggt ctgtgaaaaa
    14641 agaaaccgat atccggcgtg gaaggcattg tgttttcctg atgcatgttc acctggtctt
    14701 tgtcaccaaa taccgacgtc agatttttga ctatgacgcg acagaaaaac tacgcactta
    14761 cttttcaaat gtatgtgctg attttgaagc tgaactggtt taaatggatg gcgaaccaga
    14821 tcacgtccat ttgttaatta actatcctcc caaactggcg atatccagtc tggtaaacag
    14881 cctcaaaggc gtatcgggta ggttactgcg acgagatcga ccagatattg cagtcaggta
    14941 ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc aatagctgcg gaggtgcgcc
    15001 aatatccgtc atccgccaat acattgaaca acagcaaaca cctggttagg tggaaaaccg
    15061 cgccttatat ccccgccctg aaggacgggg ttttacggcg caccggataa atttggttat
    15121 gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa cggatgccag tccacacaat
    15181 taacgtaact ctggccagta cgctttgttg gcctccagca actcatccag aatagctttg
    15241 gcgaccgagg cgctcggtac cgttttcgat aacgtaatcg cctgccacag gtgctgatat
    15301 gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg ccacttgctg actcattaac
    15361 cctttctgaa actgcgggat atcgccgacc accagtggtt ctggtccgtt gtgcccgacc
    15421 agacacggga tctcgaccat cgcttcatca tcaaagttat ggattgcccc gttgttaggc
    15481 acaatcagca acatccgctc ctgagtgttg aaggcaattg ccgccgccag atcgacgatg
    15541 tatgacgcat gttcgtcaat ctccagcttg gccgccgctg aatttcctgc cgctgtaatg
    15601 gcgcggcaag catcgaaaac ctgtttctcg cgatgttcca ttcacctcat tcgcgcgggt
    15661 atgttcaggg ttggaatgct gaacaacata atccgggaag agataatatt tcagataggt
    15721 gttcggcaat gtatccgggt ccagtgcctg gacatcccgc gcttttgcat aggtgtcgtt
    15781 ccagctcgct tcagtatgtg tatcttgctg cggtggaaca taaccgtgct tcgagacata
    15841 ttgccgcagc tggggcatca ggtcgttgcc ctgcaaatct tcaattgatg tccaccagcc
    15901 aaagtggttc aggccgtagt agcgcacgcg catctgtttg cgatcttgca gcccaacaat
    15961 ttgcgccatc cggctttcaa taccgattgg catgtcacag atgttgagga ttttcgcatt
    16021 cgggcgcaga cgacgcgtgg cttctgcgac aatggctgcc gggttggagt agttgagcat
    16081 ccaggcattt ggtgaatatt tttccatata atccaccagt tccaggacgc cgccaatgga
    16141 acgcatgccg tacgctattc cgccgggtcc gcaagtttcc tgaccaacaa caccgtggcg
    16201 cagtgggatt ttttcatcca gttcgcgcat cgggtacttg ccgacgcgga tgtgcgccat
    16261 gacaaaatca acgtcgctga acgccacttc aggatcggtg gtgtaactaa acgcaatgtc
    16321 cggcgctttc tctttaagga tgactttaca ggcttcggca atcacttcct gccgcgcgcc
    16381 atcgttatca taaaatttca gtgcacgaag cgggaaacgc tcctgattcg ccaggagcat
    16441 caacacgatg cctggcgtaa aggtgcttcc accgcctgcg acaaccactg agaatttggt
    16501 cattatactg cctccgtaat ggcaacattt tctgctgatt gatgagaatt aattaagcta
    16561 tcgagctgtt cacgcagctg ggatacatgc agaccaatga tcacctgaat ggcatcgcca
    16621 ctacggaaga cgccgtgcgc tcccagcttt ttaaagactt cgtcatccag cgtttgtgac
    16681 atgtcatgca gtgcaatgcg tagacgcgtc gcacaattgt taatgctgga gatattgccg
    16741 accccgccca gggcttgcag gataccggca gcctgatcca gctctttttt tggctctgcc
    16801 gcggtggttt ggcctcgcga ggctttgtat tcggcttttg agtagagttt cacttccgca
    16861 tcttcacgtc ccggcgtgca catgttgaac tgcagaatca gggtgcggaa aaccacgaag
    16921 tacagcaggg taaagcacaa cccaatggcg atttgggtca gcatcatatc cgcgtggttg
    16981 ctgaacatcg ggatccagtt ttgcggtaaa acctggtcaa tcagacctcc gcccatgttg
    17041 cccaccacac caaagagata catcacggtc gacattgagg ccgcaagcac agcgtgtacc
    17101 gcaaacagca acggtgaaat gaacaggaag gtaaattcca gcggttcggt aattcccacc
    17161 agcatggcgg ttaaggttgc gggaatcagc aagcccgcca cttttacccg attttccggt
    17221 gcggcagtga agtacatcgc taaagaaatg cccacggcac caaagatttt tgagttaccg
    17281 tgcagggcaa aaccgccttc cgggaacaac gatttcagcg gctcggcgct caaactgaac
    17341 tcttgcagat gctgcgccca gtacatctga atgccgcctt caacagctgc cggacccaag
    17401 ataaacggtc cgtagatgaa gtggtgtaaa ccggttggga tcagaatacg ttcgaggaag
    17461 gtgtaaaccc agaccccaag cgcacccgcc gaacgcagga acgcttgcag agattcaatc
    17521 cccgtttgta cttttggcca gccgagcaat gtcagccagg cacaggggat catcaccagg
    17581 aaggcgataa tcaccacata agacgtcccc tggaaaatgc cgagaaaaac cggcagtttt
    17641 ttatcgaaca gacggttatg cagcgccgtc acaatgccgg aaatgataat tgcgccgata
    17701 atgctggtat cgagggtttt aatcccggcc atcattgtca gaccgctacc tgccaccgcg
    17761 tcctgagtga aatcgacgcc gaagtagctt ccccaggtca ttcccatcgc gttgatgaaa
    17821 tagttccagg tcaggaaact caccatcacc gccagacaag cacgtccctg cgcttgctta
    17881 gcaaggccaa tgggtaaacc gacagcaaaa atcagcggca tattacggaa taccgtccaa
    17941 ccgccctctt caataatgtg tacgatttgc gcgaataaac tgttcggatc ggtcagtgat
    18001 tccccgacaa acatcgggtt ttgcagcaag atggcaagac ccaccacaat cccggcgaag
    18061 ggaaacagca gcactggcgt aaacatcgcg ccgccaaagc gttgaatttg actgagcatt
    18121 tccacatcct cttttctcaa ttctgtaagt aggaagcctg attggttatg cggcaggcct
    18181 ggagcaagga taggaagatg cgatcggcag aacatgacga cccaatggga ttcgtgatcg
    18241 ctttcatgct tttcgttggg cggtagagat ctacgttttc gcgaaaaagt agacatctgt
    18301 gagcacaggt acacagcatc gaacacatct ttaaaaaaaa gatgtttttt caatcgatta
    18361 agcagaactt gtgtgcgcat tacccgggct tgcaggcaaa aaagagatct agagatgatc
    18421 tacaaaagca ttgcggagcg gttaagaatt cgacttaact ccgcagattt cacgctaaac
    18481 agccttcttc cgggtgaaaa aaagctggcg gaagagtttg cggtatcgcg gatgaccatc
    18541 cgtaaagcca ttgacctgct ggtagcgtgg gggctggtgg tccgccgcca cggtagtggc
    18601 acttacctgg tgcgcaaaga tgtactgcat caaaccgcca gtctgaccgg gctggtggag
    18661 gtgttaaaac ggcagggaaa aactgtcacc agccaggtgc tgatttttga aatcatgcct
    18721 gcgcctccgg ccattgccag ccagttacgg attcaaatca acgagcagat ctacttctcc
    18781 cgtcgcgttc gttttgtgga agggaaaccg ctgatgctgg aagacagcta tatgccggta
    18841 aaactgttcc gtaatctttc gctgcaacat ctggaagggt cgaagtttga atatattgaa
    18901 caagagtgcg ggattttgat tggcggtaat tatgaaagcc tgacgccggt gctcgccgat
    18961 agactgctgg cgcggcaaat gaaggtagcg gaacacacgc cactgctgcg gatcacctcg
    19021 ttgtcatata gcgagagcgg ggagtttttg aattattcag tgatgttcag aaatgccagc
    19081 gaataccagg tggagtacca tttacggcga ctccacccgg aaaagagtta accgatactc
    19141 cagaagagca ccgccagtaa ttggggggtg ataatgcgca ggaacatcac taacggatag
    19201 acagtggcgt aagagagcgc cgccgcaccg ctagttggat gaagattatt agcaaacgcc
    19261 agcgccggag gatcggtcat ggaacctgcc agcatcccgc acatggtcag gtaattcatt
    19321 ttggctaaca tccgcgccag aatgccaaca gtaatcagcg gaacggcggt gatcagggca
    19381 ccataaccaa tccagcttag cccttcgcca ttgaccaggg tattcacaaa atccccacca
    19441 gatttcagac caacgaccga gaggaacagc acgatcccca gctcccgcag cgcgaggttg
    19501 gcacttggcg gcataaacca gtacagcttg ccgatactgc cgatacgccc gaggatcaac
    19561 gccataatca gcagaccgcc cgccagcccc agtttcaacg cggccgggaa tcccggcaca
    19621 aagacgggaa tagaacctaa caatacgcca agcccgatgc caataaacac tggcagcatc
    19681 tgaacctgtt gcagtttttg ctgcgcattc cccagcacat tggcaacggc atcaattgcg
    19741 gacggacgcc ccaccaggtt gaggatatcg ccgaactgca ggctgatatc gccactggcg
    19801 accagttcga ccccggcacg gttcaggcgc gagataacaa cgtcatagcg ttctttaaag
    19861 tgcaggtcgc gaatacgttt tccgagcacg ttttcattgg tgaccaccac acgctcgacg
    19921 cgcaaatcag tgtctttcgt ggacagcgaa gtatcgacct cctgaccaat caccagttgc
    19981 gcattatgta aatccgctgg ctgacccacc agatgcagca aatcgcccag ttggataatg
    20041 gtatctggcg aaggaacttt tagggtttct tcgcgtttca gacgcgagca gataattttg
    20101 tcgccgttga gaatcggtac atctttaatg gctaaatcat gcaggttagg gttctcaacg
    20161 cgaatattga tagtcttgat cagcgcgccg ccattggtgc gtgaagactc gtgctgctga
    20221 gcttctgtct cgacattgac gcggaaaata acccgcaaca tccacatggt gaacaaaatc
    20281 ccgcaaatgc cgaatggata cgccatcgcg taactcatcc ccatctgatc gaccatttcc
    20341 attggtgtac ccaggtcgcg caaaatctgc tgccctgccc ccagcgctgg cgtattggta
    20401 accgcaccgg agaaaatccc cagcactacc ggcagtggaa tatcaaacag tttatgcagg
    20461 atggcggtaa ccagaccacc gatgatgacg atcagaacag caaacaggtt gaggcgtaat
    20521 ccggagacgc gcaatgaggc aaagaagccc ggccctacct gaatcccgat ggtataaacg
    20581 aacaggatca ggccaaattc ctgaataaca tgcagcatat cgctacttaa tgtcatcccc
    20641 gcctgagaaa caaaatggcc gacgatgatc ccaccaaaca gcacgccgcc aatacctaat
    20701 cctatgccgc gaaatttgac gttgccgata aacaaaccga cgactgccac caaagccaga
    20761 atactgaccg ttaatgctat atcactcatc gtccatttct cttgagataa cattttaatt
    20821 gctacggatt atggctgaat cggtgaagca gatttgcctt atagcgcaca ttattacggg
    20881 aaagaacgtg ccgaaatatc ttaaacagtc gaaatttaac gcattgttga agaagaaaga
    20941 caaaaaaagc cccaccagaa tggcggggca aagagaatag ctagttagct atttaacgcg
    21001 ggacgttcgc tgatagcgat acgctgcgct gcgatgggtt caggctcatt acgaattaaa
    21061 tcaatatgca gtaaaccgtt tacgaaagtt gcgccagaga cttccatatt ttcagccagc
    21121 gtaaagctca ggctaaatgg ctgattcata agcccttgat gcagccattt tttctcttct
    21181 tttggctgct ccggcgtgcc ttttacgctc aggcgcgtac cttccagttg aatctctaaa
    21241 tcttcctgac ggaaacctgc cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc
    21301 tcaatgttgt acggcgggaa gctctgactt tcaccggcgt tttgcagtgc gttggccagt
    21361 ttgtcaaaac cgatccattg acgcatcagt ggggataaat cgaagttacg catagtcatt
    21421 tctccttcta agaagcgagt aagtacctgc aaatccgaag attcgcatat gctccctgac
    21481 ggcgagcatg gagatgtcag gccgcgccag gcggccttaa ggaattagtt gatttcgata
    21541 cggcgcggtt ttttcgcttc cggaatcacg cgttcgagat cgatatacag caaaccattt
    21601 accaggttag cgccacgaac atgaatgttc tcagctaact ggaatttgcg ttcaaagttg
    21661 cgttcagcga tgccctggta cagataggtg cgctcttttt gttcgtcggc gtgagcacct
    21721 ttcaccacca gcagattatc ctgggcggta atttccagtt cgctctcagc aaaaccagcc
    21781 acagcgatag caatgcggta atggttttcg tctaccagtt caacgttata cggagggtag
    21841 ccgccattac tctggctctg gttgttttct aagtggttaa acaaacggtc aaatccaata
    21901 gcagaacggt aaagcgggga taaatcaaag ttacgcataa tcaatagctc ctgaaatcag
    21961 cgagaatgta agaccttcca caatggacag gtcaggtagc cagaacaccc atcaggcgcg
    22021 tcctcatcga ctacgatgta aaaatgggtc tggaaatgaa cttttcaagc cttatcagcg
    22081 gacttttttt tgcagtttat ggtctattgc atagactgag ggggcagcac gaaacgttaa
    22141 agtgtgatag ccgccacaga gcgacgtaat gatgatgtta ttttcagcaa gggatcgcta
    22201 taactcatca ggccaacacc cacgatcaac agatgaattt attatgataa gaaatgtgtt
    22261 gttagcgttc atgatatgca gcggaatggc attactcgga ggatgctcca gcgtgatgtc
    22321 ccataccgga gggaaagaag gaacgtatcc cggcacccgc gccagcgcca ccatgattgg
    22381 cgatgatgaa accaactggg gcactaagtc gctggcgatt ctcgatatgc cgtttaccgc
    22441 tgtgatggac acgcttttat taccgtggga tgtgttccgc aaagacagct cggtgcgctc
    22501 acgcgttgaa aaaagcgaag ccaacgcgca agccaccaat gcggttattc caccggccag
    22561 aatgcctgat aactgattta gcgatcagtt tcggtgatcc acagctggcc accttccatc
    22621 cacgccagcc attgaccatc cggcgagaag accacggcgt ccgcagaagg tggattggcg
    22681 tggttttcgg ttaaatactc aacctcgccg ctttgcgcat gggcacaagc aattcgatta
    22741 tccagcacaa agcccaacca ttctcctgac ggatgccagt taaatgcaga ctgaatatcc
    22801 gttttgttat gggttaactg gcgcggctcg ccgccctgtg gcgagataag ccacagttgc
    22861 acaatgccgt tatcatcacg cattaaaaac gcgatttgcg taccctgcgg attacagcgc
    22921 acccagtggc gcgggacgtt gactaacccc ggataagccc gatggtgggt aaaggttaaa
    22981 cgtcgctgca cgacgccacg cggtggcgcg ggcagggtgg tttccgttcc acttaacggc
    23041 gcatcacctg ccgctttcca gccagcttca tcttgcggca actcaacgat aaacagctcc
    23101 ggcactttct caccctttgg cgaaagtgtg tcgccaataa acgccagcgc gtgatttcct
    23161 acccatcctt cttcataagc acgattgatt tcatcactgc caggctgcgg cgtgggcgtg
    23221 gttttactca ccagcacaca ccagtggctg ccgctgtatt cacgcggatg ctgcttttgt
    23281 acgttgaccg gaccaaacgg cgcagcaaca ccgacgtttc gcaaatccag cgccggatcg
    23341 agttcatgca ttacatggtc gttataggta aagctcaccc tttcaccgtt cgggctaaag
    23401 acatgcacat ggctgccgcc gcgcagcgct cctggcgtat acggcgcggt aatatccatt
    23461 gcatcgagat tgctcacctt gccgccttca gcaatgactc cgcgccgatg atggaagtcg
    23521 taatgccatg tttcatcagg attttccggg ccgtgaataa agacatattt ctctgacttc
    23581 ggatgaacgg tcaccacgcc gacgtgtgcg ccctgtgacg cacagtagat aacctcgacc
    23641 tcaccggtat ggatattcac acgctcaatg gtctcgccgg taaacgacgc cccagaagga
    23701 cgcacgtcaa ataccagcca ctggctgtcg ggcgtccagg tattggtatt ggtgagcagg
    23761 tgattacggg gagcaaaggt tatctgtttc atggcgcggt cctgatacaa aacgggtcca
    23821 gccatcatac ttcacaagga cttcactttc agacaatagt cttttgccat cgtgatgaat
    23881 gtgagtgaaa agatggaaca tttcgacgtg gcgattattg gcctcggccc ggcagggtcg
    23941 gcgttggcac gaaagttagc cggcaaaatg caggtgatcg cgctggataa aaagcaccag
    24001 catggtactg aaggtttcag caaaccttgt ggcggtctgc tggcaccgga cgcgcagcga
    24061 tcttttattc gcgatgggct gacgcttcct gtcgatgtga tcgctaatcc gcagattttc
    24121 agcgtcaaaa ccgtcgacgt cgccgcatcg ctcacgcgta actaccagcg aagctatatc
    24181 aatattaatc gccatgcttt cgacttgtgg atgaaatcgc tgatccccgc cagcgttgag
    24241 gtttatcacg acagcctgtg tcgaaaaatc tggcgtgagg atgataaatg gcatgtcatt
    24301 tttcgtgcag acggctggga gcagcatatt accgcccgct atctggtcgg tgccgatggt
    24361 gccaactcga tggtgcggcg acatctctac ccggatcatc aaatccgtaa atatgtcgca
    24421 atccagcagt ggttcgcaga gaaacatccg gtgccgttct actcctgcat ctttgataat
    24481 gcgataactg actgttattc atggagtatc agcaaagacg gttattttat ctttggcggt
    24541 gcctatccaa tgaaagacgg tcagacgcgt ttcacgactc tgaaagagaa aatgagcgcc
    24601 tttcagttcc agtttggcaa agcggtgaaa agcgaaaaat gcacggtgct gtttccctca
    24661 cgctggcagg attttgtctg cggtaaggac aacgcctttc tgattggtga agcggcggga
    24721 tttatcagcg ccagctcgct ggaggggatt agctatgcgc tggatagcgc agacattctg
    24781 cgttcggtgt tactgaagca gccagagaag ctcaataccg cttactggcg cgccacccgc
    24841 aaactgcgtt taaaactctt cggcaagata gtaaaaagcc gatgcctgac cgcaccggct
    24901 ttaagaaagt ggattatgcg cagtggtgtg gcacatattc cacagttgaa ggattagcca
    24961 acgcgcttca catcgcccac cagcaggata taagagagcg cgccaatcaa cgcgacggcg
    25021 gagatataaa ccagtgcagg tgcgaaaccg taaccctgcg ccaggtaccc caccaccagc
    25081 ggaacggtga tgccgcccag accaccggcg aagttaaaca cgccgccggt taaaccaatc
    25141 aggcgcatcg gtgccagaga agaaaccagc gaccaggtaa ttgaagcaaa accgttaccg
    25201 aagaatgcca gcgccatcag gcacataatc atcatcggat cgttagtgta gttagcgccc
    25261 ataatgcagg tggagatcag caagccgcag ataatcggcg ttttacgcgc aaagcccagt
    25321 gaaaagccct tacgtaccag cagatccgct acccagccag agagcaggac gccgacaaac
    25381 gccgcgagga atggcaccgt ggtcataaag cctgctttca gcgccgtgat tcctttttcc
    25441 tgggttaaat agttcgggaa ccaggttaag aaaaaccaca gtgtagaagc caccgcaaat
    25501 tgcccaagat agacgccgat cagtttacga tggaacacca gtttccagtc tttggctgtt
    25561 aacggctgac gtgcctcttt cttcactggc gcatcaccat ccaccagacc gccgccatca
    25621 cgaatgtaat ccagttcagc tttgctgatg cctttggtca ggcgcggcgg ctgataaacc
    25681 ttaaaccaaa tcagcgacca gataatgccg ataccaccag tcacaatgaa cacccagtgc
    25741 cagctcaaca tctcctgaat ccagatcagc agcggagtca aaaacgccag accgacaaac
    25801 tgaccagacg tataaaaacc aacggcggag gcgcgttcat gttccgggaa ccagctggtc
    25861 accatccggt tattggtcgg gaaagcgggc gcttcgaaaa taccggttat cgcacgcaga
    25921 ccaattaatg acattaatcc cgtggcaaag ccctggaata aagtcgccac tgaccagcca
    25981 aatatcgcaa taaaataagt cacgcgagaa cctacgcgat ctaaaaacca accgccgggg
    26041 atctggcata gcgtataaag ccaggcgaag gccgaaaata catagcccat ttccgctttc
    26101 gtaatgccga actcttcctg aatatgggcg gaagccacgg ccaggttagc gcggtcaaca
    26161 taacaaatga ccaccgtaat aaagatcatc accagcgtca gataacgccg acgccccggc
    26221 tttgctgcat taacgggaat atccatagcg agctttctcc agattttggg catagcgaag
    26281 ccgctcacca tgccctgtaa tttacagagg gttatatttt tgtattgctg ttttagtgcc
    26341 cgatgagggg cttacgtggc aggaattacc actctgctac gctgttatct tcatgacgcc
    26401 agagtggatt acgccagtcc ggggcatttt tactgaactc aataacttta gcttcgtcga
    26461 tttccacgcc taagcccggt ttcgttagcg gtttaaagaa gccgccaacc atgctgaagt
    26521 cttctttgtt tttcacaaag tcgagtaact ccgcgccttt gttgtaatga attcccatac
    26581 tttgttcctg aagtaccgcg ttataggaaa caaagtcgat atgcagacaa gccgccagtg
    26641 caatcggtcc gagcggacag tgcggcgcaa gggtcacgtc ataggcttct gccattccgg
    26701 caattttgta gcattcggta ataccgcctg catgggagag atccggttgt agaatcgaaa
    26761 taccgcctgc ctccagcacg cgtttaaaat cgaaacgtga gaacatgcgc tcacccgccg
    26821 ccagtggaat atgcgtttgt gccgccaatt tcgggtagta ttcggcttgt tccgccagca
    26881 ccggctcttc aataaacagc gggcgatacg gctccagttc tttaatcagc actttcgcca
    26941 ttggcgcgct gacgcgaccg tggaaatcaa gaccaaactc aatctgattg ccaaaagctt
    27001 cacgaatttg tgccacggtg ttgactgccg catctaccgc gcgggagtta tcaattagcc
    27061 ccagttcttc acaaccgttc agtttgaagg tatcgaagcc gatttcgcgc agggttttaa
    27121 taccgtcgat cacatccgcc ggacgatcgc cgccgaccca actgtaggct ttaattttgt
    27181 cgcgaaccag gccgcccatc agttgccaga ccgacgcatt cagcacttta cctttgatat
    27241 cccataacgc ctggtcgatc ccggcaatgg cgctcatcag gattggaccg ccacgataaa
    27301 atccggcgcg atacatcact tgccataagt cattgatgcg ctatggatcc tgaccaatca
    27361 aatagtcacc cagctcgtga actgccgctt ccaccgtgcg ggcgcggcct tcaatcactg
    27421 gctcgcccca gccgacgacg ccttcatcgg tttcaatttt caggaacatc cagcggggag
    27481 gtaaacgata cgtggtaatt ttggtgattt tcattgcact gcctctcgat acgccttaac
    27541 aaatgctgct gcctgctgcg cggtgcgttc tacggactgc ccggcgcgat agagatcgct
    27601 gcctaagccc gccccagcac aacctgcgtc tatccactgc gccaggtttt ctggcgtcac
    27661 gccgccaacg gcaaagactg cgatgtccga tggcaatacc gcttttaacg ctttgatgta
    27721 ttgcggacca aaagccgatg acggaaatat tttcagcgcc tgcgcgcccg cttcgagcgc
    27781 ggtaaaggct tcggtcgccg tcgcgcagcc ggggcagacg gtcatgccgt agcccaccgc
    27841 acggcggatc acttcactat ggatattggg cgtaacgatg agctgacagc ccatcctggc
    27901 gagcgcatcg acctgttcag gtttcagtac cgtacctgcg ccaatcaacg ccttgtcgcc
    27961 atacgcatca acgatggcgg gaatgctttg ctcccattgt ggggaattca gcgggatttc
    28021 aaccgcgtcg aacccggcgt caatcaccgc gccaacatgc gccagcgcct cgtcgggctt
    28081 aatgccgcgc aaaatggcga tcagcggaag tttagtttgc cactgcatga gcgatgctcc
    28141 ttataccagc ctgaaatgcc gtgtcgcccg ccaccgccgt cacgtcgcaa cccatcgcct
    28201 gaaaggcttg ctggtagcgc gcggtcaacg atgttccggc gacaagggtg atggcgtgtt
    28261 gatgggccac atagtcgcgc atactggcga cctctgcgcc aatcaacaaa ccagagagaa
    28321 attcactgac ctgttcacgg ggaagtgttc ccagcacatg cgaggcacga acttcaaaaa
    28381 gctgcggcaa tatggcgggc gtattaagac cacgctcaag gccagccgtg aaggcatcgg
    28441 cagagttttc ctgcggcggc aaacctgcac caatcaatga gtgatttaac agtaaatgat
    28501 gtaattcacc ggtcatcacg gtgcgaaaat cgttgatttg ctggctatcg gcctgcaccc
    28561 atttgcaatg cgtaccgggc atgacataaa gagaggaagg agccagagct cgcgcgccga
    28621 tcaattgagt ttcttcgccg cgcatcacat tgtggttatc atcatgagag acgcataatc
    28681 cgggaataat ccagatattg tcgccaacag acgttaattg ttcgccaata gacgaaaaat
    28741 gggcaggaac agataaatac ggagcaactt tccagccgac attgctgccg accattcctg
    28801 ccattaccac tggcgtattc tcttcacgcc agtcggtcgt gacttctgct aacaccgcag
    28861 ccggagattt tccgttcagg cgcgtgacgc ctgcttctga ttgcctgctc tccaggcagt
    28921 ggtcgccctg ataaagccag gcgcgcagat tggtcgatcc ccagtcaatt gcgatgtagc
    28981 gagctgtcat gtgatttcct ttaaccttcg tgtcgagctg gcgatcatgg taagcgccgc
    29041 ctgctctgcc gcatcgccgt cctgatgccg tatcgcatcg aacagcgcct tatgttcctg
    29101 gagcgtttgc ggcatgttgg cctcatcgcc catccaggtt cgttcaaaaa ccgcccgctg
    29161 cagcgaactg atcgcaatgc taagttgctg taacaccggg ttatgcaccg actgcagcac
    29221 cgcctcgtgg tagcgaatat ccgcttcgtt aaacgcttcg cggtcctgat tgttggcaat
    29281 catctcgttc agcgccgatt caatctgcgc cagatcgctg gaggtcgcgc gctctgctgc
    29341 ccaacgggca atcgccggtt ccaccagatt tcgcacttcg ctcatggcac tgataagccg
    29401 tgggtcgtag tcgttttcca gcacccattg cagtacgtca gtgtcgaggt aattccactg
    29461 gttacgcggt gccacaaacg cgccgcgata acgtttcatt tcaatcagcc gtttcgccat
    29521 caacgaacgg aacacctcac ggatgatgtt gcgcgaggtt gcaaactcct cacagagttc
    29581 cgcctcagcc ggaagcggcg agcctggtac gtatttgccg tgaacgatct gtttacccag
    29641 cgtaatgacg atgcgatcgg ttttattgag agtcatggag agtccttgtg ctctgtatgt
    29701 tcttctctac tttaccccga tcgatgcata atcaggaaag tatcaatcgc agtcagacac
    29761 catcacgggg ctatcgcagg caaaggatag taatggaaca aaaggctatg tttttgtagg
    29821 ggaatcgctg gattacctta tcacagatgg tgccgatgac atcgttaaga tgctcaagga
    29881 tccagctctt aaccggcaca atattcaggt tgctgatgac gcaagatttg ttttaaatgc
    29941 ggggaaaaag aaatttaccg gcacaatatc actctattac aactggagca cagaagaaga
    30001 aaaagcacga gtgacacaat atcgttttgc ttgtggtgtt cagcactgta cccggacagt
    30061 agaaaacctg gaaggtacta tccatgagaa aaataaaaac atggattact caaacgtaat
    30121 ggctttctac catccattta aagtgcgatt ttatgaatac cattcaccca gaggcattcc
    30181 ggatggtgtt tccgcagcat tactgccagt gactgttacg ctggacatca ttactgcacc
    30241 gctgcaattt ctggttgtat atgcagtaaa ccaataatca gtaagcgggc aaacgcgttt
    30301 atgctgtttg cccgcccaca gattaattca gcacatactt ctcaatagca aacgccacgc
    30361 catcttcaag gttagatttg gtgacaaagt tcgccacttc tttcaccgaa ggaatagcgt
    30421 tatccatcgc cacaccgacg cctgcatatt caatcattgc gatgtcgttt tcctgatcgc
    30481 caatcgccat gatttcttcc ggtttaatac ctaacacgtc ggccagtgat ttcacacccg
    30541 tacctttgtt aacgcgttta tcgaggattt cgaggaagta cggcgcactt ttcagcacgg
    30601 tatatttctc tttcacttcc tgcggaatac gcgcgatagc ctggtcgagg atggcgggtt
    30661 catcaatcat catcactttc aggaactggg tattggggtc cattttctcc gcttcgcaga
    30721 acaccagcgg aatagtggca acgaaagatt catgcactgt gtagtagctg atatcgcggt
    30781 tggcggtgta cagcgtggtg cggtccaggg cgtggaaatg agaaccgact tcgcgagaga
    30841 gtttttccag gaaacgatag tcgtcatagc tgagagcagt ttgcgccacg gtgctaccat
    30901 cagctgcctt ctgtactagc gcgccgttat aagtaatgca gtagtcgccc ggctgttcca
    30961 tatgcagctc tttcaggtag ttgtgcacac ctgcatacgg gcgacccgtc gttagcacga
    31021 cattcacgcc acgggcgcga gctgcggcaa tcgcattttt aacggcgggt gaaatggtgt
    31081 gatcgggcag cagaagggtg ccatccatat cgatagcaat gagtttgata gccatgagtt
    31141 ccccaggtag attggttcct gacccatgct aacgcgattc cgctcaaaaa tcagtacaac
    31201 acccgaggga aaagggggat gcaacgcggg ttgcatgctc cccatttgct tagcggaaga
    31261 gtttcccttt cagcagttcc atacctgcgg aaagcagatc gttattggct tgtggtgaca
    31321 cttcaccttg cggtgagagc gcatcaataa tcttcggcaa ttgctctgcc agtaaactgg
    31381 aagctgtact ggtatccacg ccaagttttt gcccgagatc ggacaccgca tttgtgccga
    31441 gcgccgattc cagttgctcg ccactaaccg attgattgcc ctgttgatta ctcagccagg
    31501 ttgagagaat ggcccctaag ccgccacttt gcagtttttc cagcagcacc tgaatgccgc
    31561 cctgctcctc aacccaactt aaaatagcct gatatttccc cgcatcgcct ttcagaaagg
    31621 caccgacaac ttcatcaaaa agccccatga taatcacctg taaagcgtta cgtgttgacc
    31681 caaaaagtat agatttgcgg gatgataatt gcggattgca gaaataaaaa gggcggagat
    31741 gatctccgcc cttttcttat agcttcttgc cggatgcggc gtgaacgcct tatccggcct
    31801 acaaaatcat gaaaattcaa tacattgcaa gattttcgta ggcctgataa gcgtagcgca
    31861 tcaggcacgc tcgcatggtt agcgccatta aatatcgata ttcgccgctt tcagggcgtt
    31921 ctcttcaata aacgcacggc gcggttcaac ggcgtcgccc atcagcgtgg tgaacaactg
    31981 gtcggcagca atcgcatctt taacggtaac gcgcagcata cgacggcttt ccgggtccat
    32041 ggtggtttcc cacagctgtt ccgggttcat ctcgcccaga cctttataac gctggatgga
    32101 gaggccgcga cgggactctt tcaccagcca gtccagcgcc tgctcgaagc tggctaccgg
    32161 ctgacgacgc tcgccacgtt cgataaacgc atcttcttcc agcaagccac gcagtttctc
    32221 acccagcgtg cagatacgac gatattcgcc accggtgata aactcgtgat ccagcggata
    32281 gtcagtatcc acaccgtggg tacgcacgcg aacaatcggt tcaaacaggt tttgttcggc
    32341 attggtgtgg acatcaaact tccactggct gccgtgctgt tctttgtcgt tcagttcgct
    32401 gaccagcgcg ttcacccagc gggtaacggt ctgctcatca gagaggtcgg cttccgtcag
    32461 cgtcggctga tagataagct ctttcagcat tgctttcgga taacgacgct ccatgcggtt
    32521 gatcattttc tgcgtcgcgt tgtactcaga caccaatttc tctaacgctt cgccagccaa
    32581 tgccggtgcg ctggcgttgg tgtgcagcgt tgcgccatcc agcgcgatag agatctggta
    32641 ctgatccatc gcttcgtcgt ctttaatgta ctgttcttgc ttgcctttct tcactttgta
    32701 cagcggcggc tgagcgatgt agacgtgacc gcgttcaacg atttccggca tctgacgata
    32761 gaaaaaggtc aacagcagcg tacgaatgtg cgagccgtcg acgtccgcat cggtcatgat
    32821 gatgatgctg tgataacgca gtttgtccgg gttgtactcg tcacgaccga taccacagcc
    32881 aagcgcggtg ataagcgtcg ccacttcctg agaagagagc atcttatcga agcgcgcttt
    32941 ctcaacgttg aggattttac ccttcagcgg cagaatcgcc tggttcttgc ggttacgccc
    33001 ctgcttcgca gagccgcccg cggagtcccc ttccaccagg tacagttcgg aaagcgccgg
    33061 atcgcgttcc tggcagtctg ccagtttgcc cggcaggccc gctaagtcga gcgcaccttt
    33121 acggcgggtc atttcacgcg cgcgacgcgc cgcttcacgg gcacgggcag catcgataat
    33181 tttgccaacc acgattttcg cgtcggttgg gttttccagc aggtattctg ccagcagttc
    33241 gttcatctgc tgttcaaccg ctgatttcac ctcagaagaa accagtttgt ctttggtctg
    33301 ggaggagaat ttcgggtccg gcactttcac ggaaacgacc gcaatcaggc cttcacgcgc
    33361 atcgtcaccg gtggcgctaa ctttggcttt tttgctgtag ccttctttgt ccatgtaggc
    33421 gttcagggta cgggtcatcg ccgcacggaa gcctgccagg tgagtaccgc cgtcacgctg
    33481 cggaatgttg ttggtaaagc agtagatgtt ttcctggaag ccatcgttcc actgcaacgc
    33541 cacttcgacg ccaataccgt ctttttcagt ggagaagtag aagatattcg ggtggatcgg
    33601 cgttttgttc ttgttcagat attcaacgaa cgccttgatg ccgccttcat agtggaagtg
    33661 gtcttctttg ccgtcgcgct tgtcgcgcag acgaatggaa acgccggagt tgaggaacga
    33721 caactcacgc agacgtttcg ccagaatttc atattcgaac tcggtcacat tggtgaaggt
    33781 ttcgaggctg ggccagaaac gcaccatggt gccggttttt tcagtctcgc cggtaaccgc
    33841 caacggtgcc tgcggtacac cgtgttcgta gatctgacgg tgaattttac cctcgcgctg
    33901 gataaccagc tccagttttt gcgacagggc gtttactacc gaaacaccaa cgccgtgcag
    33961 accgccggac actttatagg agttatcgtc aaatttaccg cctgcgtgca gaacggtcat
    34021 gatcacttcc gccgccgata cgccctcttc cgggtgaata ccggtcggaa tgccgcgccc
    34081 gtcatcctgt acagagacag agttatcggc gtgaatggtg acgataattt ctttacagtg
    34141 acccgcgagc gcttcgtcga tagcgttatc taccacctcg aataccatgt ggtgcagacc
    34201 ggtgccgtca tccgtgtcgc cgatatacat acccgggcgc ttacgcaccg catccagccc
    34261 tttcaggact ttgatactgg aggagtcata agaattcgac atcaacgttt ctcgctcatt
    34321 tatacttggg ttaatccgtt attttaccct tttccacggt aaacatcttc gaattttcgt
    34381 ccgacatgtc tataacgtgt tcagcactga tcgcgctgac aaagacctgt gattgcgtcg
    34441 cttttaagcg gctggcaagc agcccgcgac gctcatcatc aagctcagag gcaaaatcat
    34501 ctatcaggta gagacaccgc cgcccgcttt cacgggtgag gaactctcct tgcgccagac
    34561 gtaaggcgca catcaacagc ttaagctgcc cacgcgataa ggtatcttcc accggcgcac
    34621 cgtcggcgcg aatgcgtaag tccgctttat gcgggccatg cgcggtgtag gttagctggc
    34681 gatcgcgttc aaaattacgt tccagcacct cagcatattc tgtctctttc tcccagccgc
    34741 gctggaaaga gaaagtcaga gaaaactcag ggagaaattg cttacaagta tcggccatat
    34801 cagccgcgat accggcgcta tactccgcgc gccaggtgct gatttgctcc gccagcggga
    34861 tcagctcttt atcccacggg cgtagctgtt cgtaacgtgt cacctggcgc agcgccgcat
    34921 tgcgctgctt gagcaatcgc ttgagattgc tccaggcggt gaaaaatccg ggttcgttgt
    34981 gaaagcatcc ccagtcgagg aatgctcttc tgtatttggg gccgccgttg agtaaagtaa
    35041 acccttctgg cgttatcaac tgcattggca tcaggtgcgc cagttccgcg accttatgcc
    35101 cgtctgtacc gtcgatgcgg actttgctgt cgccctgttt gtctttggtt aagccaatcg
    35161 ctgtctcgcg ctcttcgccc tgtaatcgcc cgtggagaac aaacgcctcc tgctcatggc
    35221 gaatgacgcg accaatctgc aaactgcgaa acgcccgacc atggccgagc gtatagatgg
    35281 cttccagcac gctggttttg ccactgccgt tggcacctac cagaaagtta aagccgggag
    35341 ataaagcgag atccgcggtt tcaatgttgc ggaaatcgcg gatcaacaag cgggtgaggg
    35401 acattacagt ctcattggca tgacaacata ggcagccgac tgtgatgcgg catcttcaat
    35461 ctgcacgctg gaaaccgaat cggtcagcat catgcggacg ttttcacatt tcagcgcgtt
    35521 cagaacatcc agcacgtagc tgacgttgaa gccgatttcc atctccgcac cgctataggt
    35581 aacgtcgagg atctcttccg cttcttcctg ttccgggttg ttggcggtga ttttcagctg
    35641 gttttcgctg acataaagac gtacgccgcg gaatttctcg ttagagagaa tcgccgcgcg
    35701 agcaaacgcc tgcttgagca gatcgcagcc agcttccaga tgtttgtccg ggttcttcgg
    35761 cagaacgcgg cgataatccg ggaagcgacc atccaccagt ttggaggtga agataaagtc
    35821 gccaacgtgg gcgcgaatgt tgttgctgcc aatctgtacg cgcagcggat tgtcgccgcc
    35881 gtcgagcata cgcatcagtt caatcacgcc tttacgcggc acgatcaccg aatggcttgg
    35941 caaagattga ccaattggca ttgaacagac tgccagacgg tggccgtcgg tcgccacggt
    36001 gcgcagttct tcgccttcgg tttcaaacag cataccgttt aagtaatagc gaacgtcctg
    36061 atgcgccata gaaaactggg tcgcttcaat cagacgcttc atcgtcgcct gcggcagggt
    36121 aaattcgact tcgctctgcc agtcatcgag gttcgggaaa tccgccgctg gcagggtaga
    36181 cagcgaaaaa cggctacgcc cggagcgcac cagcatccgt tcaccttcca gctgtacggc
    36241 aatttccgcg ccttcaggca gaccacggca gatatcaaag aatttgcgcg ccggaacggt
    36301 cgtcgctcct ggctcgtgtg gctgaaccag cgcaacgcgt gccaccattt ccatctcgag
    36361 atcagtaccg gtcagcgaca acgtaccgtc agcaacctgt aacagcagat taccgagaat
    36421 cggtagcgta ggacgaccac ctaacggccc gctcacctgt tgtagcggtt ttaataaatg
    36481 ctcacgttct acggtaaatt tcataggttt acgatgacaa tgttctgatt aaatttgaaa
    36541 aatcttcttt gatatcgtgg ctctcttcac gcaactgctc gatcttacgg caggcatgaa
    36601 gcaccgtcgt gtggtcacga ccaccaaacg catcgccaat ctccggcaga ctgtggttag
    36661 tcagctcttt cgccagcgcc atcgccatct ggcgcggacg cgccaccgag cgggatcgac
    36721 gcttggaaag gagatccgcg actttgatct tgtagtactc cgccaccgtc ttctgaatat
    36781 tgtcgatggt gaccagtttt tcctgcaatg ccagcaagtc gcgcagtgcc tcacgcacga
    36841 agtcgatggt gatcgcccgt ccggtaaagt tggcgttagc aataacgcgg ttcagcgccc
    36901 cttccagctc acgtacgtta gatcgtagac gcttggcgat aaagaacgcc acttcgcctg
    36961 gcaaacgaat gtcattttcg tcggcctttt tcatcaggat cgccacgcgg gtttccagct
    37021 ctggcggttc gatcgccaca gtcagtcccc aaccgaagcg ggatttcaaa cgatcctcaa
    37081 cgccgttgat ctctttcgga tagcgatccg aggtgagaat gatctgttga ttaccttcca
    37141 gcagggcgtt gaaggtgtgg aaaaactctt cctgagatcg ttctttatta gcaaaaaact
    37201 gaatatcgtc gatcagcagt gcatctacgg aacggtagta gcgtttaaac tcttcgatcg
    37261 cgttgttttg cagggcttta accatgtcct gaacaaagcg ctcggagtgc atataaacca
    37321 ctttggcatt cggcttgcgc gccataatgc cgttacccac cgcatgcagc aggtgagttt
    37381 tacccagacc cgtgccgcca taaaggaaca atgggttata ggcaccgcca gggttatccg
    37441 ccacctggcg agccgccgcg cgcgccagtt ggttagattt accttcaacg aagttatcaa
    37501 acgtgtgttt gacgtttacg ttagaacgat aggtcggttc tgccggggcc gggacgttat
    37561 cccaacctga gcgcgtagaa ggcgcagcac gttgcggctg cgtttgcgcc acctgtgcag
    37621 gggccgcgac gttgctcgtc actgccgctt gtggcgtttg cgtcaccggt ttggtgccga
    37681 cttcaaaacg cagctgtggg gcatccgctc cgcagaaact ggttagcagt ccattgatat
    37741 tattaaggta cttgtcccgt acccaatcga ggacaaaacg gtttggcgcg tacagggcca
    37801 gcgtgttatc gctcagttcc gcctgcaatg ggcgtatcca catactgaat tctgtggctg
    37861 gtaactcatc ctgcaatcgg gcaagacact gctgccaaag cgaaagtgac acggcggact
    37921 ccactcgaac aaaagtcgat aatgactaag gctgaaacat tcatgattgt tgacgtacgt
    37981 cgaaaagacc ctgcttgagg gtgacgcacg aaccgctgtc tgcggttata tgcccgatca
    38041 agatcctgca aaacgatcgg gaccgcggat catagcctaa actgcgcaag agatcttctg
    38101 tttctcacag attttcccga tttatccaca ggactttcca gacctcgcta agtgtaaacg
    38161 atcctgccgc gaggcgggca cgatttacgc cgcatattgg aaaatttaat gaccatagac
    38221 aaaaattggc ttaatcgatc taataaagat ctaggacgat ccttgcgctt tacccatcag
    38281 cccgtataat cctccacccg gcgcgccatg ctggttttca ctggtgtgag gtcgtacatt
    38341 ttccctgcga aaaggtgcgg aaaagcgcgg taaataagga aagagaattg actccggagt
    38401 gtacaattat tacaatccgg cctctttaat cacccatggc ttcggtgtcc atcgtttcat
    38461 ttttcggcgg atatccaata aagccattga atttattcaa gtttaggtag aaatcgccat
    38521 gaaacgcact tttcaaccgt ctgtactgaa gcgcaaccgt tctcacggct tccgtgctcg
    38581 tatggctact aaaaatggtc gtcaggttct ggcacgtcgt cgtgctaaag gccgcgctcg
    38641 tctgaccgtt tctaagtaat aaagctaacc cctgagtggt taagctcgca tttcccaggg
    38701 agttacgctt gttaactccc agtcaattca cattcgtctt ccagcagcca caacgggctg
    38761 gcacgccgca aattaccatt ctcggccgcc tgaattcgct ggggcatccc cgtatcggtc
    38821 ttacagtcgc caagaaaaac gttcgacgcg cccatgaacg caatcggatt aaacgtctga
    38881 cgcgtgaaag cttccgtctg cgccaacatg aactcccggc tatggatttc gtggtggtgg
    38941 cgaaaaaagg ggttgccgac ctcgataacc gtgctctctc ggaagcgttg gaaaaattat
    39001 ggcgccgcca ctgtcgcctg gctcgcgggt cctgatagcc ctcattcggg tctatcaacg
    39061 cctgattagt ccgctactcg ggccgcattg tcgtttcact ccaacctgtt caagctacgg
    39121 aattgaggca ttgcgcaggt ttggagtgat aaaaggcagt tggttgacgg tgaaacgcgt
    39181 attaaaatgc caccctttac accctggtgg tgacgatccc gtcccgcccg gaccatttga
    39241 taccagagaa cactaacgat ggattcgcaa cgcaatcttt tagtcatcgc tttgctgttc
    39301 gtgtctttca tgatctggca agcctgggag caggataaaa acccgcaacc tcaggcccaa
    39361 cagaccacgc agacaacgac caccgcagcg ggtagcgccg ccgaccaggg cgtaccggcc
    39421 agtggccagg ggaaactgat ctcggttaag accgacgtgc ttgatctgac catcaacacc
    39481 cgtggtggtg atgttgagca agctctgctg cctgcttacc cgaaagagct gaactctacc
    39541 cagccgttcc agctgctgga aacttcaccg cagtttattt atcaggcaca gagcggtctg
    39601 accggtcgtg atggcccgga taacccggct aacggcccgc gtccgctgta taacgttgaa
    39661 aaagacgctt atgtgctggc tgaaggtcaa aacgaactgc aggtgccgat gacgtatacc
    39721 gacgcggcag gcaacacgtt taccaaaacg tttgtcctga aacgtggtga ttacgctgtc
    39781 aacgtcaact acaacgtgca gaacgctggc gagaaaccgc tggaaatctc cacctttggt
    39841 cagttgaagc aatccatcac tctgccaccg catctcgata ccggaagcag caacttcgca
    39901 ctgcacacct tccgcggcgc ggcgtactcc acgcctgacg agaagtacga gaaatacaag
    39961 ttcgatacca ttgccgataa cgaaaacctg aacatctctt cgaaaggtgg ttgggtggca
    40021 atgctgcaac agtatttcgc gacggcgtgg atcccgcata acgacggtac caacaacttc
    40081 tataccgcta atctgggtaa cggcatcgcc gctatcggct ataaatctca gccggtactg
    40141 gttcagcctg gtcagactgg cgcgatgaac agcaccctgt gggttggccc ggaaatccag
    40201 gacaaaatgg cagctgttgc tccgcacctg gatctgaccg ttgattacgg ttggttgtgg
    40261 ttcatctctc agccgctgtt caaactgctg aaatggatcc atagctttgt gggtaactgg
    40321 ggcttctcca ttatcatcat cacctttatc gttcgtggca tcatgtaccc gctgaccaaa
    40381 gcgcagtaca cctccatggc gaagatgcgt atgctgcagc cgaagattca ggcaatgcgt
    40441 gagcgtctgg gcgatgacaa acagcgtatc agccaggaaa tgatggcgct gtacaaagct
    40501 gagaaggtta acccgctggg cggctgcttc ccgctgctga tccagatgcc aatcttcctg
    40561 gcgttgtact acatgctgat gggttccgtt gaactgcgtc aggcaccgtt tgcactgtgg
    40621 atccacgacc tgtcggcaca ggacccgtac tacatcctgc cgatcctgat gggcgtaacg
    40681 atgttcttca ttcagaagat gtcgccgacc acagtgaccg acccgatgca gcagaagatc
    40741 atgaccttta tgccggtcat cttcaccgtg ttcttcctgt ggttcccgtc aggtctggtg
    40801 ctgtactata tcgtcagcaa cctggtaacc attattcagc agcagctgat ttaccgtggt
    40861 ctggaaaaac gtggcctgca tagccgcgag aagaaaaaat cctgattcgg tgagttttcg
    40921 ctaaaataag ggcggtcagt tgaccgcctt ttttcttttc gtagggtgga taagcaccgc
    40981 gcatccgcca cacaaagcaa caggaacatc atgagcgata atgacactat cgtagcccag
    41041 gccacgcctc cgggacgtgg cggcgttggc atcctgcgca tctccggcct aaaagcccgt
    41101 gaagttgccg aaaccgtgct gggtaaactg cctaagccgc gctacgccga ttatcttccg
    41161 tttaaagacg ccgacggcag cgtgctcgat caggggattg cgctatggtt ccctggcccg
    41221 aactcgttca ccggcgaaga tgtgctggaa ctgcagggtc atggcggtcc ggtgatcctc
    41281 gacctgctgt taaaacgcat tctgaccatt cccggcctgc ggattgctcg ccctggtgag
    41341 ttttccgaac gcgcgtttct taacgataaa ctcgacttag cccaggccga ggcgattgcc
    41401 gatcttatcg acgccagttc ggaacaggcg gcccgttcgg cacttaactc gctgcaaggc
    41461 gcattctccg cacgggtcaa ccatctggtg gaagccctca cccacttgcg catttacgtc
    41521 gaagcggcaa ttgatttccc tgatgaagag atcgatttcc tctccgacgg caaaattgaa
    41581 gcccagctta atgacgtgat tgccgatctc gatgcagtgc gtgctgaagc acgtcagggt
    41641 agtttgttgc gcgaagggat gaaggtggtg attgccggac gtcctaacgc cggtaaatcg
    41701 agcctgttaa acgcgctggc gggccgtgaa gcagcaatcg taactgatat cgccggaacc
    41761 acacgtgacg tgctgcgtga gcatatccac attgacggaa tgccgctgca tatcatcgat
    41821 accgctgggc tacgtgaagc cagtgacgaa gtggaacgta ttggtatcga gcgcgcgtgg
    41881 caggaaattg aacaggccga ccgcgtgctg tttatggtcg atggcaccac aacagacgcc
    41941 gtggatccgg cagagatctg gccggaattt atcgcccgtc tgccagcgaa actaccgatc
    42001 accgtggtgc gcaataaagc cgatatcacc ggcgaaacgc tgggaatgag tgaagtgaac
    42061 ggtcacgcgt taattcgtct ctcggcaagg acaggtgaag gcgtggaggt gctgcgtaac
    42121 catctcaaac agagcatggg ctttgacacc aacatggaag gcggcttcct ggcgcgtcgt
    42181 cgccacctac aggcgctgga acaggcagcg gagcatttgc agcaaggtaa agcgcaactg
    42241 ttaggtgcct gggcgggtga actgctggcg gaagagttgc gcctggcaca gcagaactta
    42301 agcgaaatca ccggggaatt tacctctgat gatttgctgg ggcggatttt ctccagcttc
    42361 tgtattggta agtaaccgcg cttacgaagc cgtattctga ctgtcagatg cggcttcgct
    42421 tcattgttag cactcctgtt attcctcaac ccttttttta aacattaaaa ttcttacgta
    42481 atttataatc tttaaaaaaa gcatttaata ttgctccccg
//
LOCUS       DAJTYZ010000017        41075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000017 DAJTYZ010000000
VERSION     DAJTYZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.017"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            34..945
                     /locus_tag="OO596_000939"
     CDS             34..945
                     /locus_tag="OO596_000939"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HCR6716868.1"
                     /translation="MKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLIT
                     RITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGNLLNEENPHQLQQLIRLPG
                     QQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMAPLGLYEFK
                     SKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKC
                     PEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYL
                     GTPNNTYLKYNPKAKGNGVTIIRQGSN"
     gene            947..1183
                     /locus_tag="OO596_000940"
     CDS             947..1183
                     /locus_tag="OO596_000940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716869.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1356..2031)
                     /locus_tag="OO596_000941"
                     /pseudo
     CDS             complement(1356..2031)
                     /locus_tag="OO596_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(2176..2784)
                     /locus_tag="OO596_000942"
     CDS             complement(2176..2784)
                     /locus_tag="OO596_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6716870.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2968..3285)
                     /gene="metJ"
                     /locus_tag="OO596_000943"
     CDS             complement(2968..3285)
                     /gene="metJ"
                     /locus_tag="OO596_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6716871.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3562..4722
                     /gene="metB"
                     /locus_tag="OO596_000944"
     CDS             3562..4722
                     /gene="metB"
                     /locus_tag="OO596_000944"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6716872.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4725..7157
                     /gene="metL"
                     /locus_tag="OO596_000945"
     CDS             4725..7157
                     /gene="metL"
                     /locus_tag="OO596_000945"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6716873.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            7506..8396
                     /gene="metF"
                     /locus_tag="OO596_000946"
     CDS             7506..8396
                     /gene="metF"
                     /locus_tag="OO596_000946"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6716874.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8725..10905
                     /gene="katG"
                     /locus_tag="OO596_000947"
     CDS             8725..10905
                     /gene="katG"
                     /locus_tag="OO596_000947"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6716875.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10999..11904
                     /gene="yijE"
                     /locus_tag="OO596_000948"
     CDS             10999..11904
                     /gene="yijE"
                     /locus_tag="OO596_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6716876.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(11931..12548)
                     /locus_tag="OO596_000949"
     CDS             complement(11931..12548)
                     /locus_tag="OO596_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6716877.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12822..13925)
                     /gene="gldA"
                     /locus_tag="OO596_000950"
     CDS             complement(12822..13925)
                     /gene="gldA"
                     /locus_tag="OO596_000950"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR6716878.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13936..14598)
                     /gene="fsa"
                     /locus_tag="OO596_000951"
     CDS             complement(13936..14598)
                     /gene="fsa"
                     /locus_tag="OO596_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6716879.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14610..17111)
                     /gene="ptsP"
                     /locus_tag="OO596_000952"
     CDS             complement(14610..17111)
                     /gene="ptsP"
                     /locus_tag="OO596_000952"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6716880.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLWALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            17420..18499
                     /locus_tag="OO596_000953"
     CDS             17420..18499
                     /locus_tag="OO596_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6716881.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18514..18834
                     /locus_tag="OO596_000954"
                     /pseudo
     CDS             18514..18834
                     /locus_tag="OO596_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            18885..21181
                     /locus_tag="OO596_000955"
                     /pseudo
     CDS             18885..21181
                     /locus_tag="OO596_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            21147..22025
                     /locus_tag="OO596_000956"
     CDS             21147..22025
                     /locus_tag="OO596_000956"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6716882.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            22027..22368
                     /locus_tag="OO596_000957"
     CDS             22027..22368
                     /locus_tag="OO596_000957"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6716883.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22355..23206)
                     /locus_tag="OO596_000958"
     CDS             complement(22355..23206)
                     /locus_tag="OO596_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6716884.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(23421..25154)
                     /locus_tag="OO596_000959"
     CDS             complement(23421..25154)
                     /locus_tag="OO596_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6716885.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(25337..27988)
                     /gene="ppc"
                     /locus_tag="OO596_000960"
     CDS             complement(25337..27988)
                     /gene="ppc"
                     /locus_tag="OO596_000960"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6716886.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(28340..29491)
                     /gene="argE"
                     /locus_tag="OO596_000961"
     CDS             complement(28340..29491)
                     /gene="argE"
                     /locus_tag="OO596_000961"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6716887.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            29645..30649
                     /gene="argC"
                     /locus_tag="OO596_000962"
     CDS             29645..30649
                     /gene="argC"
                     /locus_tag="OO596_000962"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6716888.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30657..31433
                     /gene="argB"
                     /locus_tag="OO596_000963"
     CDS             30657..31433
                     /gene="argB"
                     /locus_tag="OO596_000963"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6716889.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            31494..32867
                     /gene="argH"
                     /locus_tag="OO596_000964"
     CDS             31494..32867
                     /gene="argH"
                     /locus_tag="OO596_000964"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6716890.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            33134..34051
                     /gene="oxyR"
                     /locus_tag="OO596_000965"
     CDS             33134..34051
                     /gene="oxyR"
                     /locus_tag="OO596_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6716891.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(34034..35434)
                     /gene="sthA"
                     /locus_tag="OO596_000966"
     CDS             complement(34034..35434)
                     /gene="sthA"
                     /locus_tag="OO596_000966"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6716892.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35755..36405
                     /gene="fabR"
                     /locus_tag="OO596_000967"
     CDS             35755..36405
                     /gene="fabR"
                     /locus_tag="OO596_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6716893.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36405..36764
                     /locus_tag="OO596_000968"
     CDS             36405..36764
                     /locus_tag="OO596_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6716894.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36804..37904)
                     /gene="trmA"
                     /locus_tag="OO596_000969"
     CDS             complement(36804..37904)
                     /gene="trmA"
                     /locus_tag="OO596_000969"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6716895.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      38018..38208
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38273..40117
                     /gene="btuB"
                     /locus_tag="OO596_000970"
     CDS             38273..40117
                     /gene="btuB"
                     /locus_tag="OO596_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6716896.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            40062..40919
                     /gene="murI"
                     /locus_tag="OO596_000971"
     CDS             40062..40919
                     /gene="murI"
                     /locus_tag="OO596_000971"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6716897.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9495 a        10237 c        11298 g        10045 t
ORIGIN      
        1 cacacgtagg gtgaagccgg gctgaagcgg gtggtgaaaa aggaacacgc ggacggcagc
       61 gtcacgcaga gtcagtttga cgccgtgggc aggctcagga cacagacgga tgccgcaggc
      121 cggacaacag aatacagccc ggatgtggtg acgggtctca tcacgcgcat caccacgccg
      181 gatggcaggg catcggcgtt ttactataac caccacagcc agttaacgtc agccaccggg
      241 cctgacgggc tggaaatacg ccgggaatat gatgaatggg gcaacctgct gaatgaagag
      301 aacccgcatc agctgcagca gcttattcgt ttgccggggc agcagtatga tgaggagtcc
      361 ggcctgtatt ataaccgcca ccgctattat gacccgctgc aggggcggta tatcactcag
      421 gatccgattg ggctgaaggg gggatggaac ctgtatacat atccgctgag cccggtgaat
      481 agcatggctc cattaggatt atatgaattt aaatcaaaaa atattgatga tattggaata
      541 tttgcattgg caatgtgtaa tggagaatca attaacgaga ataaagaata tggtggacta
      601 atatgtaaga agcaaggtga atatttcccc atgaatccga taagttcaaa tgataatgat
      661 agtgtagact tgcgaaatat aaaatgccct gaaggttcag agagagtagg cgattatcac
      721 actcacggtt tttactctga cgataaagga aataaagtaa caaaagaaaa tgatgtttat
      781 gatagtctaa atttttcaag caaagattta acgaattctt atatgaatgg aatgggaaaa
      841 aaagaataca gtagttactt gggaacacca aataacacct atctaaaata taatcccaaa
      901 gctaaaggga atggagttac aattatcagg caagggagta attaaaatga aggcaataca
      961 atgttgttat ttccttgctt tagtctccag tcatttaatg gctggagtta tgggtgatga
     1021 taatatattt ttaagtaatt cggattcagc tgctttatat tgtgtggcta aaatatttcc
     1081 agatggagaa aatattttaa tatataaagt aaatataatt aatgctagtt caaaaaaaga
     1141 tgtttttttt atgaaagccc aacatcaacc cacaatgaca taatgggggg cgggggaatg
     1201 tcatatgcct atgaatatga tgtaaaatat aatatatgtc atttgagaaa aaagagcaga
     1261 atgaggtaaa aatctttggc tgtgtattca gtctggtaat catccaagga aagcttatca
     1321 gaagggtgat gactccaatt agtagaacat atattttcga taaaagcacc gatgactttt
     1381 tcatgaagtt ctacagaacg cgagaaacag atggttctgc gggccagacg tttgattcgg
     1441 gttctcaggg tcagattgtt acgctcaatg tgctgagtaa aaatcttacc agtcagatgc
     1501 ttctccttcg gtaactctct agcgtaacta ccccattcgt cagtggtcaa catgccgatg
     1561 gtaaaagggt aagcagcgcc cgcaattcac gacaggtttc gtcagtccgg gggccaaaag
     1621 tgtaggccag aacaccacca gttttcgtgt tgtacacgta ccagagccag tgctgtcgcg
     1681 ctttactgcc gacaaagctc cattgctgac taagtttgca gatgagtgcc acatcagcat
     1741 gagcgaccgg ggatgacgtt attcttcgtg gcgagagttt tttaaagttc ggatgactgt
     1801 attaatgccg attttcagcg tacgggcagt atcgcgaacc ccggcaccgt tgaaagtcat
     1861 ttcagcgatt tggtctttaa ctcccggttt acggggctcg taacagtaac tgagctgaaa
     1921 tacccggtga cagtcacggc cgcggaatcg ctcatgtctt tttgggttct gcccgtgacg
     1981 gtaaacctga acggactggc aacgggggca atgaaggtta actgtgacca tgaaacaacc
     2041 ttaaaagcga gtattatatc cttaattcaa ctaattggag gcatcaccgt ttaccggagc
     2101 gacagcttgc ggaaaggtcg tgcaactaca aagcaacaaa cagactacgg cgggaattat
     2161 ttttttcatc atccattacc acgaaaacag cggccactct ttggcgactg tggtgagttg
     2221 tggcgcgtcg aaaacggctt gtggtgaaac taccgtttcc tcaagcggaa tatttttgcc
     2281 gtagcgttct ttgagcttag cctggaccag cgggttgctg agatcgaact ctttcaactt
     2341 ctgctgctcg cccacgcgca ttcccagcgc gttctggtaa accttccaca ccacttccga
     2401 acagtactgg cgatcgtcgc tccatgagaa gctgaaatca tacggtttac cgagataacg
     2461 ttttgccgtt tgcgccagtt tttgctgttg ttcaacactc agtccgcctt caacgcggcg
     2521 aacaacgtat ttgccctttt caccatgggc gatccactgc ttgagcgggg ggtatttcac
     2581 cgggccgacc gcttcaaaaa cgtagggctt tttgttgcgt atcaccagca taccggtgtg
     2641 gctataatcg gaatgggtcg ccagttggat cgctttactt tgcgatgagc gagagatctg
     2701 aaagatgatg tcgccggttt gtggctgcca ggcaaaggca ggtacagaaa ccagcaggct
     2761 gaggatcagc agcctgtttt tcatagttaa acgtccatgt ataaaaagcg gtgggtcgca
     2821 gacaacgtgc tcgttgttta tgccggatgc ggcgtgaacg ccttatccgg cctacaagtt
     2881 cgtgcaaatt caataaattg caatatgacg taggcctgat aagcgtagcg catcaggcga
     2941 ttccactccg cgccgctctt ttttgcttta gtattcccac gtctccgggt taatccccat
     3001 ctcacgcatg atctcttttg ccgcttccgg gatttcgtcg ctgcgctctt tacgcagatc
     3061 ggcatcatcc ggcaaaggtt gcccggtaaa ggcatgcaga aacgcttcgc acagcagctc
     3121 gctgttggta gcgtgacgca ggttgttcac ctgacgacgc gtgcgttcat cggtgaggat
     3181 ttttaacacc ttaagaggaa tggaaaccgt aatctttttg acttgttcac tcttcttgcc
     3241 gtgctcagcg tatgggctga tatattcgcc gctccattca gccatgagat acttaatcct
     3301 cttcgtcaat aaattgagac cagaccacac aatttatgtg ggtactgacc gtaaacccgc
     3361 atagtttacc gtacaggcgt taccgtgaca tcgtgtaatg cacctgtcgg cgtgataatg
     3421 catataattt taacggctat ttgggatttg ctcaatctat acgcaaagaa gtttagatgt
     3481 ccagatgtat tgacgtccat taacacaatg tttactctgg tgcctgacat ttcaccgaca
     3541 aagcccagga aacttcatca catgacgcgt aaacaggcca ccatcgcagt gcgtagcggg
     3601 ttaaatgacg acgaacagta tggttgcgtt gtccctccca tccatctttc cagcacctat
     3661 aactttaccg gatttaatga accgcgcgcg catgattact cgcgtcgcgg caacccaacg
     3721 cgcgatgtgg ttcagcgtgc gctggcagaa ctggaaggcg gtgctggcgc agtacttact
     3781 aataccggca tgtccgcgat tcacctggta acgaccgtct ttttgaaacc tggcgatctg
     3841 ctggttgcgc cgcacgactg ctacggcggt agctatcgcc tgttcgacag tctggcgaaa
     3901 cgcggttgct atcgcgtgtt gtttgttgat caaggcgatg aacaggcatt acgggcagcg
     3961 ctggcagaaa aacccaaact ggtactggta gaaagcccaa gtaatccatt gttacgcgtc
     4021 gtggatattg cgaaaatctg ccatctggca agggaagtcg gggcggtgag cgtggtggat
     4081 aacaccttct taagcccggc attacaaaat ccgctggcat taggtgccga tctggtgttg
     4141 cattcatgca cgaaatatct gaacggtcac tctgacgtgg tggctggcgt ggtgattgct
     4201 aaagacccgg acgttgtcac tgaacttgcc tggtgggcaa acaatattgg cgtgacgggc
     4261 ggcgcgtttg acagctatct gctgctacgc gggttgcgaa cgctggtgcc gcgtatggag
     4321 ctggcgcagc gcaacgcgca ggcgattgtg aaatatctgc aaacccagcc gttggtgaaa
     4381 aaactgtatc acccgtcgct gccggaaaat caggggcatg aaattgccgc gcgtcagcaa
     4441 aaaggctttg gcgcaatgtt gagttttgaa ctggatggcg atgagcagac gctgcgtcgt
     4501 ttcctgagcg ggctgtcgtt gtttacgctg gcggaatcat tagggggagt ggaaagttta
     4561 atctctcacg ccgcaaccat gacacatgca ggcatggcac cagaagcgcg tgctgccgcc
     4621 gggatctccg agacgctgct gcgtatctcc accggtattg aagatggcga agatttaatt
     4681 gccgacctgg aaaatggctt ccgggctgca aacaaggggt aaaaatgagt gtgattgcgc
     4741 aggcaggggc gaaaggtcgt cagctgcata aatttggtgg cagtagtctg gctgatgtga
     4801 agtgttattt gcgtgtcgcg ggcattatgg cggagtactc tcagcctgac gatatgatgg
     4861 tggtttccgc cgccggtagc accactaacc agttgattaa ctggttgaaa ctaagccaga
     4921 ccgatcgtct ctctgcgcat caggttcaac aaacgctgcg tcgctatcag tgcgatctga
     4981 ttagcggtct gctacccgct gaagaagccg atagcctcat tagcgctttt gtcagcgatc
     5041 tggaacgcct ggcggcgctg ctcgacagcg gtattaacga cgcagtgtat gcggaagtgg
     5101 tgggccacgg ggaagtatgg tcggcacgcc tgatgtctgc ggtacttaat caacaagggc
     5161 tgccagcggc ctggcttgat gcccgcgagt ttttacgcgc tgaacgcgcc gcacaaccgc
     5221 aggttgatga agggctttct tacccgttgc tgcaacagct actggtgcaa catccgggca
     5281 aacgtctggt ggtgaccggg tttatcagcc gtaacaacgc cggtgaaacg gtgctgctgg
     5341 ggcgtaacgg ttccgactat tccgcgacac aaatcggtgc gctggcgggt gtttctcgtg
     5401 taaccatctg gagcgacgtc gccggggtat acagtgccga cccgcgtaaa gtgaaagatg
     5461 cctgtctgct gccgttgctg cgtctggatg aagccagcga actggctcgt ctggcggctc
     5521 ccgttcttca cgcccgtact ttacagccgg tttctggcag cgaaatcgac ctgcaactgc
     5581 gctgtagcta cacgccggat caaggttcca cgcgcattga acgcgtgctg gcctccggta
     5641 ctggtgcgcg tattgtcacc agccacgatg atgtctgttt gattgagttt caggtgcccg
     5701 ccagtcagga tttcaaactg gcgcataaag agatcgacca aatcctgaaa cgcgcgcagg
     5761 tacgcccgct ggcggttggc gtacataacg atcgccagtt gctgcaattt tgctacacct
     5821 cagaagtggc cgacagtgcg ctgaaaatcc tcgacgaagc ggggttacct ggcgaactgc
     5881 gcctgcgtca ggggctggcg ctggtggcga tggtcggtgc aggcgtcacc cgtaacccgc
     5941 tgcattgcca ccgcttctgg cagcaactga aaggccagcc ggtcgaattt acctggcagt
     6001 ccgatgacgg catcagcctg gtggcagtac tgcgcaccgg cccgaccgaa agcctgattc
     6061 aggggctgca tcagtccgtc ttccgcgcag aaaaacgcat cggcctggta ttgttcggta
     6121 agggcaatat cggttcccgt tggctggaac tgttcgcccg tgagcagagc acgctttcgg
     6181 cacgtaccga ctttgaattt gtgctggcag gcgtggtgga cagccgccgc agtctgttga
     6241 gctatgacgg gctggacgcc agccgcgcgt tagccttctt caacgatgaa gcggttgagc
     6301 aggatgaaga gtcgttgttc ctgtggatgc gcgcccatcc gtatgatgat ttagtggtgc
     6361 tggacgttac cgccagccag cagcttgctg atcagtatct tgatttcgcc agccacggtt
     6421 tccacgtcat cagcgccaac aaactggcgg gagccagcga cagcaataaa tatcgccaga
     6481 tccacgacgc cttcgaaaaa accgggcgtc actggctgta caatgccacc gtcggtgcgg
     6541 gcttaccgat caaccacacc gtgcgcgatc tgatcgacag cggcgatact attttgtcga
     6601 tcagcgggat cttctccggc acgctctcct ggttgttcct gcaattcgac ggtagcgtgc
     6661 cgtttaccga gctggtggat caggcgtggc agcagggctt aaccgaacct gacccgcgtg
     6721 acgatctctc tggcaaagac gtgatgcgca agctggtgat tctggcgcgt gaagcaggtt
     6781 acaacatcga accggatcag gtacgcgtgg agtcgctggt gcctgcccat tgcgaaggcg
     6841 gcagcatcga ccatttcttt gaaaatggcg atgaacttaa cgagcagatg gtgcaacggc
     6901 tggaagcggc ccgcgaaatg gggctggtgc tgcgctacgt ggcgcgtttc gatgccaatg
     6961 gcaaagcgcg tgtgggcgtg gaagcggtgc gtgaagacca tccgctggca tcactgctgc
     7021 cgtgcgataa cgtctttgcc atcgaaagcc gctggtatcg cgataatccg ctggtgatcc
     7081 gtgggcctgg cgctgggcgc gacgtcaccg ccggggcgat tcagtcggat atcaaccggc
     7141 tggcacagtt gttgtaattt cagaaattta ataatgcccg gtactcatgt tttcgggttt
     7201 atggtttcta atgaaatata ttgaattatc ataggattag gccggattaa gcgtttacga
     7261 cgaatccggc aagaagcaat aagtacatgg ttagtttata tttgcagtcc ggtttgcttt
     7321 gcataccgga ttttcttttt cttaccatcc tgaagttttt tcatcttccc tgatttttcc
     7381 tcaccatcat tggtcatttt tcggttgacg cccttcggct tttccttcat ctttacatct
     7441 ggacgtctaa acggatagat gtgcacaaca caacatataa ctacaagcga ttgatgaggt
     7501 aaggtatgag cttttttcac gccagccagc gggatgccct gaatcagagc ctggcagaag
     7561 tccaggggca gattaacgtt tcgttcgagt ttttcccgcc gcgtaccagt gaaatggagc
     7621 agaccctgtg gaactccatc gatcgcctta gcagcctgaa accgaagttt gtatcggtga
     7681 cctatggcgc gaactccggc gagcgcgacc gtacgcacag cattattaaa ggcattaaag
     7741 atcgcactgg tctggaagcg gcaccgcatc ttacttgcat tgatgcgacg cccgacgagt
     7801 tgcgcaccat tgcacgcgac tactggaata acggtattcg tcatatcgtg gcgctgcgtg
     7861 gcgatctgcc gccgggaagt ggtaagccag aaatgtatgc ttctgacctg gtgacgctat
     7921 taaaagaagt ggcagatttc gatatctccg tggcggcgta tccggaagtt cacccggaag
     7981 caaaaagcgc tcaggcggat ttgcttaatc tgaaacgcaa agtcgatgcc ggagccaacc
     8041 gcgcgattac tcagttcttc ttcgatgtcg aaagctacct gcgttttcgt gaccgctgtg
     8101 tatcggcggg cattgatgtg gaaattattc cgggaatttt gccggtatct aactttaaac
     8161 aggcgaagaa atttgccgat atgaccaacg tgcgtattcc ggcgtggatg gcgcaaatgt
     8221 tcgacggcct ggatgatgat gccgaaaccc gcaaactggt gggcgcgaat attgccatgg
     8281 atatggtgaa gattttaagc cgtgaaggag tgaaagattt ccacttctat acgcttaacc
     8341 gcgctgaaat gagttacgcg atttgccata cgctgggtgt tcgacctggt ttataaatag
     8401 tgtggctttt gtgaaaatca cacagtgatc acaaatttta aacagagcac aaaatgctgc
     8461 ctcgaaatga gggcgggaaa ataaggttgt cagccttgtt ttctccctca ttacttgaag
     8521 gatatgaagc taaaaccctt ttttataaag catttgtccg aattcggaca taatcaaaaa
     8581 agcttaatta cgatcaattt gacctacatc tctttaacca acaatatgta agatctcaac
     8641 tatcgcatcc gtggattaat tcaattataa cttctctcta acgctgtgta tcgtaacggt
     8701 aacactgtag aggggagcac attgatgagc acgtcagacg atatccataa cacgacagcc
     8761 actggcaaat gcccgttcca tcagggcggt cacgaccaga gcgcgggggc gggcacaacc
     8821 actcgcgact ggtggccaaa tcaactccgt gttgacctgt taaaccaaca ttctaatcgt
     8881 tctaacccgc tgggtgagga ctttgactac cgcaaagaat tcagcaaatt agattactac
     8941 ggcctgaaaa aagatctgaa agccctgctg acagaatctc agccgtggtg gccagccgac
     9001 tggggcagct acgccggtct gtttattcgt atggcctggc acggcgcagg tacttaccgt
     9061 tcaatcgatg gacgcggcgg cgcgggtcgt ggtcagcaac gttttgcacc gctgaactcc
     9121 tggccggata acgtaagcct cgataaagcg cgtcgcctgt tgtggccaat caaacagaaa
     9181 tatggtcaga aaatctcctg ggccgacctg tttatcctcg caggtaacgt ggcgctggaa
     9241 aactccggct tccgtacctt cggttttggt gccggtcgtg aagacgtctg ggaaccggat
     9301 ctggatgtta actggggtga tgaaaaagcc tggctgactc accgtcatcc ggaagcgctg
     9361 gcgaaagcac cgctgggtgc aaccgagatg ggcctgattt acgttaaccc ggaaggcccg
     9421 gatcacagcg gcgaaccgct ttctgcggca gcagctatcc gcgcgacctt cggcaacatg
     9481 ggcatgaacg acgaagaaac cgtggcgctg attgcgggtg gtcatacgct gggtaaaacc
     9541 cacggtgccg gtccgacatc aaacgtaggt cctgatccag aagctgcacc gattgaagaa
     9601 caaggtttag gctgggcgag cacttacggc agcggcgttg gcgcagatgc cattacctct
     9661 ggtctggaag ttgtctggac ccagacgccg acccagtgga gcaactattt cttcgaaaac
     9721 ctgttcaaat atgagtgggt acagacccgc agtccggctg gcgcaatcca gttcgaagcg
     9781 gtagacgcac cggaaattat cccggatcca ttcgatccgt cgaagaaacg taaaccgacg
     9841 atgctggtga ccgacctgac gctgcgtttt gatcctgagt tcgagaagat ctctcgtcgt
     9901 ttcctcaacg atccgcaggc gttcaacgaa gcctttgccc gtgcctggtt caaactgacg
     9961 cacagggata tggggccgaa atctcgctac atcgggccgg aagtgccgaa agaagatctg
    10021 atctggcaag atccgctgcc gcagccgatc tacaacccga ccgagcagga cattatcgat
    10081 ctgaaattcg cgattgcgga ttctggtctg tctgttagtg agcgggtatc ggtggcctgg
    10141 gcatctgctt ctaccttccg tggtggcgac aaacgcggtg gtgccaacgg tgcgcgtctg
    10201 gcattaatgc cgcagcgcga ctgggatgtg aacgccgcag ccgttcgtgc tctgcctgtt
    10261 ctggagaaaa tccagaaaga gtctggtaaa gcctcgctgg cggatatcat cgtgctggct
    10321 ggtgtggttg gtgttgagaa agccgcaagc gccgcaggtt tgagcattca tgtaccgttt
    10381 gcgccgggcc gcgttgatgc gcgtcaggat cagactgaca ttgagatgtt cgagctgctg
    10441 gagccaattg ctgacggttt ccgtaactat cgcgctcgtc tggatgtttc caccaccgag
    10501 tcactgctga tcgacaaagc ccagcaactg accctgaccg cgccggaaat gaccgcgctg
    10561 gtgggcggga tgcgcgtact gggtgccaac ttcgatggca gcaaaaacgg cgtcttcact
    10621 gaccgcgttg gcgtattgag caatgacttc ttcgtgaact tgctggatat gcgttacgag
    10681 tggaaagcga ccgacgaatc gaaagagctg tttgaaggcc gtgaccgcga aaccggcgaa
    10741 gtgaaataca ctgccagccg tgcggatctg gtatttggtt ctaactctgt cctgcgtgcg
    10801 gtggctgaag tttacgccag cagcgatgct cacgagaagt ttgttaaaga cttcgtggcg
    10861 gcatgggtga aagtgatgaa cctcgaccgt ttcgacctgc tgtaatctga ccttcttcag
    10921 cgactgcctt tcaggcagtc gctgaagttt ctttaccggc gtatagtgtc cacaggaaaa
    10981 ctacacactg gatctctcat gtctgccgca ggaaagagca acccactggc aatcagtggc
    11041 ctggttgtgc tcacacttat ctggagttat agctggattt tcatgaagca agtcaccagc
    11101 tacatcggtg ccttcgactt taccgcctta cgctgcattt tcggcgctct cgttttattc
    11161 atcgtccttt tattacgtgg tcgcggaatg cgcccgacac cgtttaaata caccttagcc
    11221 attgccctgt tacaaacctg cgggatggtt ggcctggcgc agtgggcgct ggtcagcggc
    11281 ggagcgggga aggtggcgat cctaagctat accatgccgt tctgggtggt gattttcgcc
    11341 gcgttgtttc tcggtgaacg cctgcgacgt gggcaatatt tcgcgatact gattgccgct
    11401 ttcggcttat ttttggtgtt gcagccgtgg caactcgatt tctcttcgat gaaaagtgcc
    11461 atgctggcaa tcctctccgg cgtcagttgg ggggcgagcg cgattgttgc taaacgtctg
    11521 tatgcccgtc atccgcgcgt ggatttattg tcgttaacat cctggcagat gctctatgcg
    11581 gcgctggtga tgagtgtggt cgctttactg gtgccgcaac gtgaaattga ctggcagccc
    11641 accgtgttct gggcgctggc ctacagtgcg attctggcga cggcactggc gtggagctta
    11701 tggttgtttg tattgaaaaa cttgcctgcc agtattgcca gcttaagcac actggccgtt
    11761 cccgtttgcg gcgtactctt ttcctggtgg ctgctcggcg agaatccggg ggccgttgaa
    11821 ggtagcggta ttgtgctgat tgtgatggca ctggcgctgg tgagccgtaa gaaaaaagaa
    11881 gccgttagtg taaaaaggat ctgaattttt tcttcatgtg gggcgatcgc ttatttaaca
    11941 aaataacgat aatgccccac catgcgccag ctaaacagca catcttcttc ctgcgcacct
    12001 gcgccaatgt tatgtatcac cagcggcgta ccgtcgcggg cgaagccatc tgaaaccacc
    12061 ccaatatgtg ccagcccgtt atctaatcgc caggagacaa tatcgcccgc ttgatagtca
    12121 ctggggttct tgctgatggg gcgtgtttta tcgtggtggg taaaccaggt ttccagatta
    12181 ggcacccgac ggtgatcgat gttgctgtcc gggcgcttta actgccattt ttgcgggtac
    12241 tcggcaaaat tcttcgccat atcttcgtgc accagtttct gcaaatcgac cttctggctg
    12301 cgcaatgcac ggatcaccac atcggaacat accccgcgtt tttgcgggac atcaccgcca
    12361 ggataggtaa gctgcatata cgccggatcg taaaatagcg tgctgccgat ttgctgtctg
    12421 gcaccgtctg cgatggcaag gttggtattg gcctggattt gtaccacggt tggtggaacg
    12481 gcgggagatt ttaaggagtg gctggtaaat gccgttagca ggctgagcag cgccagtgaa
    12541 gctttcatct ggttcgtccg tgaaaataag agtcacagat tcgaccttcc gggataacga
    12601 ttgttctgta gagaaaatgt ccagaagcgg ataaaaaact cctgaacaac gtgggagttt
    12661 cagattactg gcacagagtg ccggatgcgg cgtgaacgcc ttatctgtcc taaaaaaaaa
    12721 gaaaattcaa tatattgcag agattgcgta ggcctgataa gcgtagcgca tcaggcaatt
    12781 ttgcgttcaa actcccggac aagccgggag tttggagtag gttattccca ctcttgcagg
    12841 aaacgctgac cgtactggtc ggctaccagc agagcggcgt aaacctgatc tggcgtcgcg
    12901 ccgccaggca tgttgtggat ggtttcacat tctgcacatg ccgcttcagc cacaattcgc
    12961 attttcgccg ggacatcttc tttaatatcc agttgagcga gagttattgg caaacctacc
    13021 gcatggctaa gtgcagctac ggtttcgatt tcctcaaccg gtgcgttttc cagaaccagc
    13081 tgcgtcagcg taccaaatgc cactttttca ccgtgataat agtgatgcgc gtccgggata
    13141 gcggtcaggc cgttatgcac tgcgtgcgcc gcagccagac caccactttc aaaaccaaca
    13201 ccgctcaaat aggtgttcgc ttcaatcacg cgctccagcg ccggagtcac tacatgctgt
    13261 tcggcagcaa gcatcgcttt ttcgccttct tccagcaggg tgttgtagca cagttcagcc
    13321 agtgccagcg cagcctgggt gcacttgccg cccgccatgg tggtcgcgcc gctacgagag
    13381 caggcacgcg cttcaaacca ggttgccagc gcatcgccga tacccgccgc taacagacgt
    13441 gcaggtgcgc cagcgacgat tttggtgtcg acaatgacca tattcgggtt atttggcaac
    13501 agcagatagc ggtcaaactc accctcatcg gtgtagataa cagacaatgc gctgcacggt
    13561 gcatcggtag aggcgatagt cggtgcgatc gctaccggaa cacccatgaa atgtgccagt
    13621 gctttggctg tatcgagggt ttttccgcca ccgataccga gaattgcgcc acactgcgca
    13681 gtctccgcga tgccacgcag acggtcgatc tcattttgcg aacatccacc gccaaacggc
    13741 gcaatttcta ctaccagtcc agcatcttta aagcttttct cgacagtgga ttgagcaaaa
    13801 cctaaaacaa atttgtcacc caccactaac cagcgttctg ccagcggctt caggtattcg
    13861 cccagacgat taatcacatc agcgccctgg atgtatttac ccggtgattg aataatgcgg
    13921 tccataattg ctcctttaga gatgagtagt gccaaatgcg gcattccagt cgtgttcgaa
    13981 cttctctata gctgactcta ccgcaggggt gttgagcatt tgttgcgcta catctaaggg
    14041 cagggtgatg gattcacatc ctgccagtaa acagtccagc gcctgacgcg gcgttttaaa
    14101 gctggctgcc agcaccatgc tttctggcgc gtgcatttct aacagcgctt gcagctcctg
    14161 aaccgtacga atgccgtctc cgccctgggc atctacgcgg ttaacatacg gagcaacgta
    14221 ttttgcccct gccagcgcgg ccagtaaccc ttgtgcagca ctgtacactg cggtgccaag
    14281 cgtagtaata ccctcttttt tcagtatttt aattgctgcc agaccttcgg aagtcaccgg
    14341 gattttcacc acaatacccg gaatagcgtc gcgcaggcgc ttcgcttctt ccaccatccc
    14401 ctgcgcgtcg cggctcatgg tctgagcaaa cagaatgccc tcatcaccaa tcgctttttg
    14461 cagacgcggc agcacttccc atatggactc cttgctggca gcgataatgc tcgggttagt
    14521 tgtcaccccg gcaatgggga atatgcgtgc cagacgttcg acttctgcga cgttagcggt
    14581 gtccagatac agttccatga tgtttccttt tacagttcca gttcatgttg cagcaggctg
    14641 gcgatagcgt cggcagaggc ggcgttaacc agcgcgttac ggaattcttc gtgcatgatg
    14701 cggcgagcga ggcgcgagaa aatgcgcatg tgctgatcgc ccgcagcgtg tttgttcagg
    14761 gttaacatga tgatgaattg cgcttcatca tcaccccagc gcaccggcgc ttgcagacgc
    14821 gcaacgctga tggtggattg ctcaatgtgt tctgatttgc tgtgtggaat ggcaaaacta
    14881 aagcccagac cggtagagaa aacggcctcg cgcgcccaca agtcggcttc cagtttacgc
    14941 ggatagcggc agcgccctgc cagcagcagg ttatcggtca tgcctttgag cacttcttct
    15001 ttgctgcgcc agtcgctttc cagtgtgatg cactcggcgg tgaccagcgg tgcgtcttgt
    15061 tgggtcatgc ggaattgcgc cagcaggtgc tccacttcca gcgaagtacg gcaggccatt
    15121 gcctggttga gcaacttgcg gcactcacgg ctatcaagtt gcgccatccg cgctttcgcc
    15181 gccggaattg atggtgcgct catgctgagt tcatccagcc ctaagccgac cagcaacggc
    15241 agcacggaac ctttcgctcc cagctcaccg cacagaccaa tccatttacc ctggcgatgc
    15301 accgcctgca cggcgtaatc gagcgcccac aagaatgccg gattcaggct gttgtagtga
    15361 cgagtaacct tagcgttatc gcgatccact gccagcagat attgcgtcag gtcattacta
    15421 ccaatactaa agaaatcaat ctcttcgcag cattgatcga tgatgaacat caccgacggc
    15481 acttccagca taatgccgag ctggatcttc tcatcaaacg gaatgtgttc gttacgtagt
    15541 tgctgtttgg cttctgccag tttttctttc acccataaga tctcttccat tgaggagatc
    15601 atcgggatca tgattttcag gctgccgtga gcggaggcgc gcaggatcga gcgtagttgt
    15661 gtggtaaata acgacgcgta ttcttcataa atacgcacgg cgcgataacc gaggaacggg
    15721 tttgcctctg cgggaatgtt tagataatca acgggtttgt cgccgccaat gtccatagtg
    15781 cgcacaataa tgctgcgtcc gttggcagac tccagcgcct gacaaaaaat gttgtacagc
    15841 tcgctttcgc ccggtgcact ggtgcgatcc atatagagca tttcagtgcg gaacaaacca
    15901 acgccttccg caccattgcc gaatgcggcc tgcgcttcca cggagtgagc gatgttagcg
    15961 gcaatttcaa tgcggatacc gtcagcggta cgggcttgtt gggtcagcca gacacgctgt
    16021 tgctcacgca gggcgtcctg tacgcgggct tcttgctgat aataacgagc tacggcttct
    16081 cctggctcaa ccacaatcgc cccggcgttg ccgtcgatat aaatcgtttg atgctgccac
    16141 ggagtgaggg catcaatatc cacaccaacc agcgtcggaa tgttgaacga acgggcaagg
    16201 atcaccgtat gtgaggtggt gccgccactt ttcagcaaca gacctttgag gtgattttta
    16261 tccagttcga ggaactggct gggggtcagt tcatcagcca tacaaatggc ggactgcgtc
    16321 agtttgcccg gtgccgggaa gcgttgctca ccgtagattt gctggagtaa ctggaagcag
    16381 acgtcgcgta cgtccagggc acgttcttgc aggtagctgc tgctggaacg ggcgaactct
    16441 tcgcaaaagt gattcgcact ggcgacaatc gcttcggcgc agcttaatcc ctcgctgacg
    16501 cctgccagta agtgctgacg taaagatgta tcgccagcca gggatcggtg agcttccagt
    16561 atcgcgctgg tagcaccgtc gctatccagc agacgaaact caatgttttt cagtaccagc
    16621 gtcaggccgt tttccagtgt ggattgctcg gcgtcaacgt ctttggctgc gggaagatta
    16681 ctcagcgcat tgagatctaa agaagagatc ggcgtcagaa tgccgcctgc actaccgctg
    16741 cacacggtgc gggcgcggat aatttgcgga ttcagattgg tcagtgaaac cggcagtggt
    16801 tccagttcgt cagatttaac ttccgccagc ggcgcatcgc agtgggggaa ttcatcgcgc
    16861 agccattggc ttaaacgctg gtgcgcttcc tgttcgtcgg ccccggaaat cagtaactgg
    16921 cagttatcgc ccgccagcgt atcggtgcca atcagcgcaa gggcgctttt ggcgttgccc
    16981 ttgcggtcag tgtgcaggtt atgccactca atttgtgatg aaaaagtatt acacagcgtt
    17041 tcaacgtggc ttgccggacg cgcatgtacg ccgttaggta gctcacaaat aaattccaca
    17101 atcagggcca ttgcctctct cccggtacgg ttttctgttt acaggataag agcgcacggc
    17161 aacggcctgc catgtgacaa atctgccaaa agctggacaa atgtaatgta accgtcaatt
    17221 tgcgacgcgt ctcacaagac gccgttttgc ggcatgcttc cggtttatcg caagttatga
    17281 ggcggatcgc atttttgtac tgatattaca aaagtccagt aaatggcctt tttatccact
    17341 gtttgctccg cctgcgattg cctattgttt cctccatcaa aaatatggac atgggctttc
    17401 gcaagcccgg gagcagctta tgaatgagtt ggtgcagatc ctgaaaaata cccgtcagca
    17461 tttaatgacg ggcgtttcac acatgattcc cttcgtggta tcgggcggta ttttgctggc
    17521 ggtttccgtc atgttgtatg gcaaaggcgc agtgccggat gccgtagccg atccaaatct
    17581 gaaaaaactg tttgatatcg gcgttgcggg cttgacgctg atggtgcctt tcctcgccgc
    17641 ttacattggt tattccattg cagaacgttc tgcgctggct ccatgcgcta tcggtacctg
    17701 ggttggtaac agctttggtg cgggcttctt tggtgcactg atcgccggga ttatcggcgg
    17761 catcgtggtg cattacctga agaaaattcc ggtgcataaa gttctgcgct cggtgatgcc
    17821 tatcttcatt attcctatcg tcggcacact gattaccgca ggcatcatga tgtggggctt
    17881 gggcgagcct gtaggggcgt tgaccaacag cctgactcag tggcttcagg ggatgcagca
    17941 gggcagcatt gttatgctgg cggtgatcat gggtctgatg ctggcgttcg atatgggcgg
    18001 tccggttaac aaagtggcct atgccttcat gctgatttgc gttgctcagg gtgtttatac
    18061 cgtggtggcc atcgctgccg tgggtatttg tatcccgccg ctggggatgg ggctggcgac
    18121 gctgattggt cgtaaaaatt tctccgcaga agagcgcgaa accggcaaag cggcactggt
    18181 gatgggctgt gttggcgtta ctgaaggggc gattcctttc gccgctgccg atccgctgcg
    18241 tgtcattcct tccatcatgg tcggttcagt ttgtggtgca gtaactgcgg cgctggtcgg
    18301 tgcgcagtgc tatgcaggct ggggtggtct gattgtgctg ccggtggttg aaggcaagct
    18361 gggttatatc gcagcagtgg ctgtcggagc agtggtgacg gctgtttgtg tgaacgtgct
    18421 gaaaagtctg gcgcgtaaaa atgggtcttc gactgatgaa aaagaagacg acctggattt
    18481 ggattttgaa attaattaat tgaggaacga gccatgacga aaattattgc agtaaccgca
    18541 tgtccttcag gtgttgccca tacctatatg gcagcagagg cgctggaaag tgccgcgaaa
    18601 gcgaaaggct gggaagtgaa agtagaaacc taggggtcaa ttggtctgga aaatgaactg
    18661 actgcggaag atgtggcgag cgctgatatg gtgattctga ccaaagatat cggcatcaag
    18721 tttgaagagc gttttgctgg caagaccatc gtgcgcgtca acatcagcga tgcagtaaaa
    18781 cgtgctgacg ccattatgag caaaattgaa gctcacctgg cgcaaaccgc ttaagttctt
    18841 ctcccgctcg caagggcggg ttcgctttcc cacaggagtt cctcatgacg aatcgtatct
    18901 ctcgcctcaa aactgcactg tttgccaata cccgtgaaat ctcgctggag cgggcgctgc
    18961 tttataccgc cagccatcgg caaaccgaag gcgaaccggt gatattgcgc cgggcgaaag
    19021 caacagcgta tatccttgaa catgttgaaa tttcgattcg tgatgaagaa ctgattgccg
    19081 gtaaccgcac cgtaaaaccg cgcgccggga ttatgtcgcc ggaaatggac ccttactggc
    19141 tgctgaaaga gctggatcaa ttcccgacgc gtccgcagga ccgctttgct atcagcgaag
    19201 aagataaacg tatctaccgt gaagagttgt tcccgtactg ggaaaaacgt tcgatgaaag
    19261 atttcatcaa cgggcagatg acagatgaag taaaagccgc gaccaacacg cagattttca
    19321 gcatcaacca gacggataaa ggccaggggc acattattat tgattaccca cgcctgttga
    19381 atcacggtct gggtgagctg gtggcacaga tgcagcaaca ttgtcagcaa cagccggaga
    19441 atcactttta tcaggccgcg ttgttactgc tggaagcctc gcagaaacac attttgcgtt
    19501 acgccgaact ggcggaaacg atggcggcaa actgcacaga tgcacagcgt cgcgaagaac
    19561 tgctgactat tgcggagatc tctcgccata acgccgaaca taagccgcag acgttctggc
    19621 aggcgtgcca gttattctgg tacatgaaca tcattctgca atacgaatcc aacgccagtt
    19681 cgctgtcttt agggcgcttc gaccagtata tgttgccgtt ctatcaggca tctttaaccc
    19741 agggcgaaga tccggcgttc ctgaaagaac tgctcgaatc tttatgggtg aaatgcaacg
    19801 acatcgtgct gttgcgctcc accagcagcg cgcgttattt cgcaggtttc ccgaccggct
    19861 ataccgcact gctcggcggg ttaaccgaga acggacgtag cgcggtgaac gtgctttcgt
    19921 tcctttgcct tgacgcctat caaagcgtgc aattaccgca accgaacctc ggcgtgcgca
    19981 ctaacgcctt gatcgacacg ccgttcctga tgaaaaccgc cgaaaccatt cgcctcggca
    20041 ccggtattcc gcaaatcttt aacgatgaag tggtggtacc agcgttcctc aaccgtggcg
    20101 tttcgctgga agatgcgcgc gactattccg tagtgggctg tgtggaatta tctattcccg
    20161 gcagaaccta cggcttgcat gacatcgcga tgtttaatct gctgaaagtg atggaaatct
    20221 gcctgcatga aaatgaaggc aatgctgcgc tgacttatga aggtttactg gagcagatcc
    20281 gcgccaagat cagccactac atcaccctga tggttgaggg cagcaatatt tgtgatatcg
    20341 gccatcgcga ctgggcacct gtaccgctgc tctcgtcttt tatcagcgat tgtctggaaa
    20401 aaggtcgcga tattaccgat ggcggcgcgc gttataactt ctccggcgta caggggatcg
    20461 gtatcgccaa cctgagcgat tctctccatg cgttgaaagg gatggttttt gagcaacagc
    20521 gtttaagttt tgacgaattg ctgtcggtat taaaagccaa ctttgcaacg ccagaaggcg
    20581 aaaaagtccg tgctcgctta attaaccgct tcgagaaata cggtaacgat atcgacgagg
    20641 tggataacat cagcgccgaa ctgttacgcc actactgcaa agaagtggaa aaataccaga
    20701 acccgcgcgc ggttacttca cgccgggatc gtataccgtt tctgctcacg tcccgttggg
    20761 atcggtggtt ggcgcgacgc cagacggtcg ttttgccggg gaacaactgg cagacggcgg
    20821 cttgtcacct atgctgggcc aggacgcaca agggccaaca gcggtactga agtcagtcag
    20881 taagctcgat aatacgctgc tgtctaacgg tacgttgctg aacgtgaaat tcactccggc
    20941 gaccctggaa ggtgaagcgg gattacgcaa actggccgac ttcttacggg cgtttaccca
    21001 gcttaagttg cagcatattc agtttaacgt ggtgaacgcc gacacgttgc gggaagcgca
    21061 acagcgccca caagattatg ccgggctggt ggtgcgcgtt gccggataca gcgccttctt
    21121 tgtcgaactg tcgaaggaga tccaggatga catcatccgc cggacagcgc atcagctgta
    21181 acgttgtgga aacgcgccgc gatgatgtgg cgcgcatttt caacattcag cgttattcac
    21241 tgaatgacgg cgagggcatt cgtacggtgg tcttttttaa aggctgtccg catctttgcc
    21301 cgtggtgtgc taatccggag tcgatctccg gcaaaatcca gacggtacgc agagaggcga
    21361 aatgtctgca ctgtgcgaaa tgtttgcgtg atgcggatga atgcccctcc ggggcgtttg
    21421 aacggattgg tcgcgatatc agccttgacg ctctggaacg ggaagtgatg aaagatgaca
    21481 ttttctttcg cacgtccggc ggcggcgtca cgctttctgg cggcgaagtg ttaatgcagg
    21541 cggagtttgc tacccgtttt ttacagcgac tgcggctgtg gggtgtgtca tgcgccattg
    21601 aaactgccgg agacgcgcca gcaagcaagc tgttaccgct ggcgaaattg tgcgatgaag
    21661 tgctgttcga tttaaaaatt atggacgcga ctcaggcgcg ggatgtggtg aagatgaacc
    21721 tgccacgcgt gctggagaat ctgcgtttgc tggtgagtga gggtgtcaac gtgatcccgc
    21781 gtttaccgtt gatccctggt ttcacgctca gcagggagaa tatgcagcag gcgctggatg
    21841 tactgatccc gctgaaaatc aggcagatcc atctgttacc gtttcatcag tacggcgaac
    21901 cgaaataccg cctgctgggg aaaacatggt cgatgaaaga ggtgcctgcg ccgtcgtcag
    21961 ccgatgtgac aacgatgcgc gaaatggcag aacgggccgg atttcaggtt accgtgggag
    22021 gttaaaatgg catacctggt ggcagtaacc gcctgcgtaa gcggcgtggc gcatacttat
    22081 atggcggcgg aacggctgga aaagttgtgc cagttagaga agtggggcgt cagcattgaa
    22141 actcagggcg cgctgggaac ggagaatcgt ttagcggacg aggatatccg tcgggcggat
    22201 gttgctctgt tgattacgga tatcgagctt gccggtgccg agcgatttga acattgccgc
    22261 tatgtgcaat gcagcatcta cgcattcctg cgtgagccgc agcgggtaat gagcgcggtg
    22321 cgcaaagtgc tttctgcgcc gcagcaaacc catcttattc tggagtagtc ggtttttctg
    22381 tcagttggct gtggtactgc cggcgatatt ccgacggcga gcgttcggtg tttttacgaa
    22441 acagacggca gaagtagttg ctgtcgacaa agccgcaggc gtgcgccact tcttttacct
    22501 tcaggtcata gcctttcaat aacgtcttag cgtgctccag tcgcgtgtga ttcaggtatt
    22561 cgttaaaacc aatggccccc gttttttgaa acaggtgcga gagatagttt ggtgaaatat
    22621 agaatgcctg tgcgacagat tcgcgggtaa gcgcggaggc atagcgttcg tcgatataat
    22681 cgcgaatagc ttcaaatagt gcctggctgc gtgaggcggt ctggatttgg ctgccgagca
    22741 aatcgcggca gtggctaagc aggctggcta cgataagcct tgccgtttgc tgctcctgcg
    22801 gctgcatttg catttcattg agcgtttgta gcagaaaaga acctatacgt gggccgcgtc
    22861 gggcgacgtg ttgcttcgcc agattttgat attgtttgcc atcccattgc acgacgctga
    22921 agccgagttg ctgtttgcca aacagcacgc taaaggtggt agcgggtgtt ttccattgtg
    22981 ggaaattcca gccaccagcg gcaacgtaca gcacatcgcc gggaactaac gttgctccag
    23041 cgccggtatc aacaaactca ccttccagca caatttccag ccgtggaaaa tcgacctgat
    23101 acgcaagatc gggcaccggg ccgttactgc tggcaaagta aatctggcgc agggacagcg
    23161 ggccgttgat caggcgggag agcagatagc tgacgccgtg atacatgtta ttcccttagg
    23221 tatgtagacg ggaaaacagt aactggcatt attaaaaaag gccacctgaa aagtgtggcc
    23281 tgaggggagt tcgatgtaaa cattgcggca gttgtattgc cggatgtggc gtaaacgcct
    23341 tatccagcct accgccgcga tctgtaggcc ggataagacg cgtcaagcgt cgcatccggc
    23401 aaatagcgcc tggctgataa ttactgatta cctacctggt cgccatacgg cagagtgtcg
    23461 taatcgatca gcgcgttttt cttgtacggg ttaccaatcc agcgggtagt ttctttgaac
    23521 tgcggattca ccactgaacg ggttggatcg taaccgtcgt aagataagcc cgccaaatct
    23581 gaccaggtgt ggatcagttc cgccaggctg tatttacgat cgacatcctg tgagaaatca
    23641 cggggatgag tcgcttgcca tttttccgac gtccacagca ggaacggaat ggtgtacatg
    23701 tggcgcgtcg ggttgtcttc gttacgcccc tgagttttgt gcggcggcgt gtcgtaaacc
    23761 tcttcaccgt ggtcagagaa gtaaaccagg aatccgttcg ggtctgccgc tttaaagtct
    23821 ttaatcaggc tggcaaccac atgatcgtta tacaggttag cgttgtcata atcgttatat
    23881 gattccagct cttctgcgct taatcctggc ggaacatgat cggtattgcc atcaaacttg
    23941 ccctgatttt ccgggtagcg gtatttgtat ttgatatgcg tacccagcag atgaacgatg
    24001 atcagtttct tcggcgcagg gtcattcagc acatcctgga acggcttcag cacgttggtg
    24061 tcgtattcac gcgcactctg cgtacgttgc tggttcatgt agtactgctt gtcggtctgg
    24121 cgcgaaaata ccgtcagcat ggtattgcgg gcggtcatcg tctgctggtt ggtgatccag
    24181 aaggttttat aacccgcctg tttcatcatg ttcatcagcg acggctgcgt cagatacaga
    24241 tccgggttct tttcattggc aaaggtcagc gcctgttgca ggatttcaat ggtgtacgga
    24301 cgagacgtaa ctacgttatt aaacacggtc agattcggat cggttttatg cagcgcatcc
    24361 agctccggcg tggtttcacg cggataaccg tacagactca tgcgtccgcg ctgggtcgac
    24421 tcgccaatca ccagcactaa agtgcgcggt tcgttacccg attcatcttt gaaattagcc
    24481 agtggcggca aggcattatt ttcattcagt aactttgtta gcgagtttag ttgctgacga
    24541 tactgataat agccggtcag gaattgccac ggtgcggcag gctccatacg cgaggccagg
    24601 ttatccaacg ttttctcaaa cggcttgttt ttgataaacg tattcatggc gatcggatgc
    24661 agaatcaagc cataaagcag ggcaaaagag acaacataac gccacggctt tggaatatag
    24721 accgggcgca ggcgtgtcca cagcagaact gccaccgccg tataggccag cgcgataagc
    24781 acaattttca ggctgaaata ctggcttaaa tactcgctgg cttcgttggt gttggtttcg
    24841 aacatcacaa acagaacgct ctgcgagaac tcctgcccgt agatgacgta gtagcatagc
    24901 gccgccagag aggccgccca tagcaccacg ccgattactg cggcaataat tttaatccgc
    24961 ttcggaaaga ggaataccgg gatcaaccac agcgagctga ataacagcga gtcgcgaatg
    25021 ccgttggtgc cactataacc actgatgtaa ataatggcct gtagcagagt agagaaaaac
    25081 caaaagtaga gcagtgccca acccagggct ttccagctaa aaaggggttt agcctggact
    25141 tctgtggaat gcatagtaag aacctgtctt gaaaaaaata tcgccgaatg taacgacaat
    25201 tccttaagga tatctgaagg tatattcaga atttgaataa aatgcagaca gaaatatatt
    25261 gaaaacgagg gtgttagaac agaagtattt cagaaaaccc tcgcgcaaaa gcacgagggt
    25321 ttgcagaaga ggaagattag ccggtattac gcatacctgc cgcaatcccg gcaatagtga
    25381 ccattaacgc ttgttcgacg cgaggatccg gttcctggcc ttctttttct gcctggcggg
    25441 agcggtgcag caactcggcc tgcaatacgt tcagcgggtc ggtgtaaata ttccgtagct
    25501 gaatagactc tgcaatccac ggcagatcgg ccatcagatg ggaatcgttg gcaatcgcca
    25561 gcaccacttt gatgtcttct tcttgcaggt tgcgtaactc tttacctaac ggccacagtg
    25621 ctttgtctac cagacgttga tcatagtatt ccgccaacca caggtctgct ttggcgaaga
    25681 ccatctccag catgccgaga cgcgtcgaga agaatggcca atcgcggcac atggcttcca
    25741 gctcgctctg tttgccatct tcgaccactt tttgcagcgc cgtacctgca cccagccagg
    25801 cggggagcat caggcggttt tgcgtccagg caaaaatcca cggtatggcg cgcagcgact
    25861 cgacgccgcc ggttgggcga cgtttcgccg gacgtgaacc caacggcagt ttgcccaatt
    25921 cttgttccgg cgtagcggag cggaagtaag gcacaaaatc tttgttttca cgtacgtagc
    25981 cacggtagag atcgcaggag atgactgaca gttcatccat aatgcgacgc cagctctctt
    26041 tcggctcagg tggtggcagc aggttggctt ccagaatcgc cccggtgtaa agcgacaggc
    26101 tgctgacggt gatttctggc agaccatatt taaagcggat catctcgccc tgttcagtca
    26161 cgcgcaggcc acctttcagg cttcctggcg gttgtgacag cagcgccgca tgggcaggtg
    26221 cgccgccgcg accaatggaa ccgccgcgac cgtggaacaa cgtcagctca atacccgctt
    26281 tttcgcaggt tttgattaat gcatcctgtg cctgatattg cgcccaggaa gctgccatca
    26341 cgcccgcatc ttttgctgag tcggaatagc caatcatcac catctgtttg ccctgaatca
    26401 ggccgcgata ccagtcgata ttgagcagct gggtcatgac atcgttggcg ttgttcaggt
    26461 catcgagggt ttcaaacagc ggagcaaccg gcatcgcaaa cccgataccc gcttctttca
    26521 gcagcaggtg gacagccagt acgtcggacg gcgttttcgc catcgagatc acgtaggcgg
    26581 caatggagcc ttgcggcgct tcggcaatca cctggcaggt atcgagcact tcgcgcgttt
    26641 cggcgcttgg ttgccagttg cgcggcagaa gcggacgttt ggagttcagt tcgcggatca
    26701 ggaacgcctg tttgtcggcc tctgaccagc tttcgtagtc gccgataccg aggtagcggg
    26761 tcagctcgcc cagcgcttcg gtatggcggg tgctctcctg acggatatca atacggacca
    26821 gcggtacgcc gaaacatttc acgcggcgca gggtgtcgag cagatcgccg ttggcgataa
    26881 tacccatgcc acacgcctga agtgactggt agcaagcgta gagcggttcc cacagttctt
    26941 cgttttgtgt cagcagccct tctggttttg gcagttcttc gcctttcagg cgcgcttcca
    27001 gccatgcctg tgtcgccatc aggcgagaac gcaggttttt catcagatag cgatacggtt
    27061 ctgcggcacc ttcttcgcca accagcgcca gcagttcagg ggtcgcttca accatcgaca
    27121 gttcagaaac cagaacctga atatctttca ggaacagatc ggtggctttc cagcgactga
    27181 gtagcaggac gtggcgggtg atatcggcag tgacgttcgg gttgccgtcg cggtcaccgc
    27241 ccatccacga agtaaaacgg accggaacaa attcgacggg cagtttgtag ccgaggttct
    27301 cttccagttg ttcgttcagt tcgcgcaagt aattcggtac gccttgccac aggctgtttt
    27361 ctactacggc aaagccccat ttggcttcat ctaccgggct tggacgcagc ttacggattt
    27421 catcagtatg ccatgactgg gcgattaact ggcgcaggcg acgcatcagc tggttgtgtt
    27481 cgtagtcagc gatatctttg ttatcgagct gttttaagca ggcgttcact tccaccattt
    27541 tgtggatcag tgtacgacgg gtaatttcgg ttgggtgagc cgtgaggacc agttccagcg
    27601 acagcgattc cactgctttt ttgatggtgt cttcgctcag ttccggctgg tttttcagtt
    27661 tgcgcagggt gcgggcgatc acttccgggt tgctggcagc ttcgcctttc ggcgaaatgc
    27721 tgtggtattg ctcggcggtg ttggccaggt tcaggaactg actaaacgca cgcgcaacgg
    27781 gcagcagctc gtcgttcgac aaattttgta aggtggtgag caactcctgg cggttagcat
    27841 cattgccagc gcgtgaagat ttcgacaact tacggatagt ttctacgcgt tcaagaatgt
    27901 gttctcccaa cgcatccttg atggtttctc ccagcacttt gccgagcata ctgacattac
    27961 tacgcaatgc ggaatattgt tcgttcatat taccccagac accccatctt atcgtttgat
    28021 agccctgtat ccttcacgtc gcattggcgc gaatatgctc gggctttgct tttcgtcgtc
    28081 ttttataaag ccacgtaaaa gcggtgacgt caaatgctgc gaaatcgctt cagcaaacga
    28141 ataaatagca ggaatttacg ccattaaatt cacgacgctt taaataagcg taacttatgg
    28201 aaatgttaaa aaatcgccat acataacacc aaaggtgtag gtcggataag atgcgcaagc
    28261 atcgcatccg acattattgc ggcaccggag tttggcaacg gtgccggatg cggcgcgagc
    28321 gccttatccg gcctacgttt taatgccagc aaaaatggtg aattacctgg ataatcaact
    28381 cacgggtggg tttaataaac cgcgtctcca gatattcatc aggctgatga gcttgattga
    28441 tggaacccgg accgagcacc agcgtcgggc ataacgtttg aataaacggc gcttcggtac
    28501 agtaattcac cacttcggtt tttgctccga gcaatttctc aaccacttca accagttgat
    28561 gattcggtgg gcattcatag ccagggatcg gcggatgcag ctcgtcgacc gtcagacgac
    28621 ccggccagcg ttcgctcacc ggagccaatg catcgttgag caaaccatta agttcattga
    28681 gtgtcatgct aggcagcgga cgaatatcca tatgcaactc acagcaagcg caaatacggt
    28741 tagaagcgtc gccaccgtga atatgcccga ggttgagcgt agggtatggc acggtaaatg
    28801 cttcgtagtg ataacgttct ttcaggttat cgcgcaattg caaaatatgc ccgatggcgt
    28861 cgtgcattag ttcgatagcg ttaactccgc gtgctggatc gctggagtgc cccgactggc
    28921 cctgaatacg gatggcgtta gagatatgac ctttatgtgc gcgtaccggt tgtagtgacg
    28981 tcggttcgcc aatgatggcg caatccgggc gcagggcggt agtttcggca aaataacgcg
    29041 ctccggccat actggtttct tcatcagcag tcgccagaat gtagagcggt tttttcagtt
    29101 tcgtgacgtc gacatcgcgt agcgtatcaa ggataaacgc aaaaaagcct ttcatgtcgg
    29161 cggtgcctaa gccgtaaagc ttgccgtcat gctccgtcag tgtaaacgga tcgcgcgtcc
    29221 agcgaccgtc atcaaatggc accgtatcgg tatgccccgc cagcaacaag ccgccagccc
    29281 cctgtccgat acttgccagc atattgaatt tgttgcgagt tcctggcaca ggctgcactt
    29341 ccacattgaa gcctaaatct ttaaaccagt ccgccagcag agtgattaaa tctgcattgc
    29401 tttgatcgag tgcctcttcc gtggcgctta ttgaaggtgt ggcaatcaga gcacggtaaa
    29461 tctcgataaa tggcggtaat ttgtttttca ttgttgacac acctctggtc atgatagtat
    29521 caatattcat gcagtattta tgaataaaaa tacactaacg ttgagcgtaa taaaacccac
    29581 cagccgtaag gtgaatgttt tacgtttaac ctggcaacca gacataagaa ggtgaatagc
    29641 cccgatgttg aatacgctga ttgtgggtgc cagcggctac gctggcgcag agctagtgac
    29701 ctatgtaaat cgccatccgc atatgaacat aaccgctttg actgtttcag cgcaaagcaa
    29761 tgatgcggga aagttaatct ccgatttgca tccgcagcta aaaggcatcg ttgagctgcc
    29821 gttgcagccg atgtcggata tcagcgagtt tagcccaggg gtggacgtag tgtttctcgc
    29881 caccgcccat gaagttagcc acgatttagc gccgcagttt cttgaagcgg gctgcgtggt
    29941 gttcgacctt tccggtgcgt ttcgtgttaa cgacgccacc ttctatgaaa aatattacgg
    30001 ctttacccat caatacccgg aactgctgga acaggcagcc tacggtctgg cggagtggtg
    30061 cggtaataaa ttaaaagaag cgaatttgat tgcggtgcca ggctgttatc cgacggcggc
    30121 acagctggcg ctgaaaccgt tgattgatgc cgatcttctt gacctcaatc agtggccggt
    30181 gatcaacgcc accagcggcg tgagcggtgc aggacgtaag gcagcgattt caaatagttt
    30241 ttgtgaagtt agcctgcaac cgtatggcgt ctttactcat cgccatcaac cagagatcgc
    30301 cacacacctc ggtgctgacg ttatcttcac cccacatctg ggcaatttcc cgcgcggcat
    30361 tctcgaaacc attacctgcc gcctgaaatc gggtgtgacc caggcgcaag tcgcgcaagt
    30421 gttacagcag gcgtatgccc ataaaccgct ggtgcggctg tatgacaaag gcgttccggc
    30481 gctgaaaaat gtcgttgggc tgccattttg cgatatcggg tttgccgttc agggcgagca
    30541 tctgattatt gtggcgaccg aagacaactt actgaaaggc gcggcggcac aagcggtaca
    30601 gtgcgccaat attcgtttcg gctatgcgga aacgcagtct cttatttaag ggtgcaatga
    30661 tgaatccatt aattatcaaa ctgggcggcg tactgctgga tagtgaagag gcgctggaac
    30721 gtctgtttag cacactggtg aattatcgtg agtcacatca gcgtccgctg gtgattgtgc
    30781 acggcggcgg ttgcgtggtg gatgagctga tgaaagggct gaatctgccg gtgaaaaaga
    30841 aaaacggcct gcgggtgacg cctgctgatc agatagacat tatcaccgga gcactggcgg
    30901 gaacggcaaa taaaaccctg ttggcatggg cgaagaaaca tcagattgcg gccgtaggtt
    30961 tgtttctcgg tgacggcgac agcgtcaaag tgacccagct tgatgaagag ttaggtcatg
    31021 ttggactggc gcagccaggt tcgcctaagc ttatcaactc cttgctggag aacggttatc
    31081 tgccggtggt cagctccatt ggcgtaacag acgaagggca actgatgaac gtcaatgccg
    31141 accaggcggc aacggcgctg gcggcaacgc tgggcgcgga tctgattttg ctctccgacg
    31201 tcagcggcat tctcgacggc aaagggcaac gcattgccga aatgaccgcc gcgaaagcag
    31261 aacaactgat tgagcagggc attattactg acggcatgat agtgaaagtg aacgcggcgc
    31321 tggatgcggc ccgcacgctg ggccgtccgg tagatatcgc ctcctggcgt catgcggagc
    31381 agcttccggc actgtttaac ggtatgccga tgggtacgcg gattttagct taagttttgt
    31441 tggccggagg cgcagctttc cggcattgaa tttcaaaata aggaaacaga gttatggcac
    31501 tttggggcgg gcgttttacc caggcagcag atcaacggtt caaacaattc aacgactcac
    31561 tgcgctttga ttaccgtctg gcggagcagg atattgttgg ctctgtggcc tggtccaaag
    31621 ccctggtcac ggtaggcgtg ttaaccgcag aagagcaggc gcaactggaa gaggcgctga
    31681 gcgtgctgct ggaagatgtt cgcgccaggc cacaacaaat ccttgaaagc gacgccgaag
    31741 atatccatag ctgggtggaa ggcaaactga tcgacaaagt gggccagtta ggcaaaaagc
    31801 tgcataccgg gcgtagtcgt aacgatcagg tagcgactga cctgaaactg tggtgcaaag
    31861 ataccgttag cgagttactg acggctaacc gacagctgca atcggcgctg gtggaaaccg
    31921 cacaaaacaa tcaggacgcg gtcatgccag gttacactca cctgcaacgc gcccagccgg
    31981 tgacgttcgc gcactggtgc ctggcctatg ttgagatgct ggcgcgtgat gaaagccgtt
    32041 tgcaggatgc gcttaagcgt ctggatgtca gcccattagg ctgtggcgcg ctggcgggaa
    32101 cggcctatga aatcgaccgt gaacagttag caggctggct gggctttgct tcggcgaccc
    32161 gtaacagtct ggacagcgtt tctgaccgtg accatgtgtt ggaactgctg tcggctgccg
    32221 ctatcggcat ggtgcatctg tcgcgttttg ctgaagatct gattttcttt aacaccggcg
    32281 aagcggggtt tgtggagctt tctgaccgcg tgacttccgg ttcatcatta atgccgcaga
    32341 agaaaaaccc ggatgcgctg gagctgattc gcggtaaatg cggtcgggtg cagggtgcgt
    32401 taaccggcat gatgatgacg ctgaaaggtt tgccgctggc ttacaacaaa gatatgcagg
    32461 aagacaaaga aggtctgttc gacgcgctcg atacctggct ggactgcctg catatggcgg
    32521 cgctggtgct ggacggcatt caggtgaaac gtccacgttg ccaggaagcg gctcagcagg
    32581 gttacgccaa cgccaccgaa ctggcggatt acctggtggc gaaaggcgta cctttccgcg
    32641 aggcgcacca tatcgtgggt gaagcggtgg tggaagccat tcgtcagggc aaaccgctgg
    32701 aagatctgcc gctcgacgag ttgcagaaat tcagcccggt gattggcgaa gatgtctatc
    32761 cgattttgtc gctgcaatcg tgcctcgaca agcgtgcggc aaaaggcggc gtctcaccgc
    32821 agcaggtggc ccaagcgatt gcttttgcgc aggcgcggtt ggggtaagaa catttatatg
    32881 tacaaatttg agcctggctt atcgccgggc ttttttatgg caaaaaaaag cggatcctgg
    32941 agatccgcaa aagttcacgt tggctttagt tattcgagtt gagaaactct cgaaacgggc
    33001 agtgacttca agggttaaaa gaggtgccgc tccgtttctg tgagcaatta tcagtcagaa
    33061 tgcttgatag ggataatcgt tcattgctat tctacctatc gccatgaact atcgtggcga
    33121 tggaggatgg ataatgaata ttcgtgatct tgagtacctg gtggcattgg ctgaacaccg
    33181 ccattttcgg cgtgcggcag attcctgcca cgttagccag ccgacgctta gcgggcaaat
    33241 tcgtaagctg gaagatgagc tgggcgtgat gttgctggag cggaccagcc gtaaagtgtt
    33301 gttcacccag gcgggaatgc tgctggtgga tcaggcgcgt accgtgctgc gtgaggtgaa
    33361 agtccttaaa gagatggcaa gccagcaggg cgagacgatg tccggaccgc tgcatattgg
    33421 tttgattccc acggttggac cgtacctgct accgcatatt atcccgatgc tgcaccagac
    33481 ctttccaaag ctggaaatgt atctgcatga agcacagacc caccagttac tggcgcaact
    33541 ggacagtggc aaactcgatt gcgtgatcct cgcgctggtg aaagagagcg aagcattcat
    33601 tgaagtgccg ttgtttgatg agccaatgtt gctggctatc tatgaagatc acccgtgggc
    33661 gaaccgcgaa tgcgtaccga tggccgatct ggcaggggaa aaactgctga tgctggaaga
    33721 tggtcactgt ttgcgcgatc aggcaatggg tttctgtttt gaagccgggg cggatgaaga
    33781 tacacacttc cgcgcgacca gcctggagac gctgcgcaac atggtggcgg caggtagcgg
    33841 gatcacttta ctgccagcgc tggctgtgcc gccggagcgc aaacgcgatg gggttgttta
    33901 tctgccgtgc attaagccgg aaccacgccg cactattggc ctggtttatc gtcctggctc
    33961 accgctgcgc agccgctatg agcagctggc agaggccatc cgagcaagaa tggatggcta
    34021 tttcgataaa gtgttaaaac aggcggttta aaccgtttaa cgcagctacc cgataggctt
    34081 ccgccatggt cgggtagtta aaggtggtgt tgacgaagta ctcaatagtg ttgccgccac
    34141 ctttctgttc cataatcgcc tgaccgatat gaataatttc ggcagcgcgc tcgccaaagc
    34201 agtgaatacc cagaatctct tttgtttccc gatggaacaa aattttcagc gtgcccacgt
    34261 tcatgccgac gatttgtgcg cgtgccagat gtttaaactg ggcgcggccc acttcatatg
    34321 gcactttcat cgcggtcagc tgctgttcgg ttttgcccac agagctgatt tccgggatgg
    34381 tgtaaatgcc ggtagggata tcttcaatca gatgcgcggt ggcttcgcct tttaccagcg
    34441 cctgcgcggc aatgcgcccc tggtcatagg ccgctgatgc caggctcgga taaccaatca
    34501 cgtcgcccac cgcgtaaacg tgcggctgtg cggtctgata catgctgttg accttcagct
    34561 gtccgcgact gtcagtttcc agcccaatgt tctgtaacgc cagcgaatcg gtattaccgg
    34621 tacgaccgtt ggcatagagc aggcagtcag ctttcagttt tttacccgac ttcagatgca
    34681 tgatcacacc gtcgtcacag ccttcgatct tctcgtactc ttcgttgtga cgaatcacta
    34741 cgccactgtt ccagaagtga taggagagag aatctgacat ctcttgatcg agaaatgcca
    34801 gcaggcgatc gcgggtgttg atcagatcca cttttacatc cataccgcgg aagatcgacg
    34861 catattcaca gccgatcact ccagcaccat agataagtac atggcgcggt tcgtggtgca
    34921 tgctaagaat tgagtcgctg tcgtaaatgc gtggatgggt gaaatcaaca tctgttggat
    34981 gatatggacg agagccgcag gcaataacaa atttttcagc ggttagtgtt tcgacgctgc
    35041 cgtccgggca atccagcgcc aacgtatgct cgtcaacaaa gcgagcgttc ccctgcaata
    35101 tttcacagtg attacgttcg taaaatccct gacgcatgcg cgtttgttga ttaatcacgt
    35161 tatcggcatg gttaaggata tcggcaaaag aagagcggag cagtcgcgaa tggtcgctgt
    35221 atagtgggtt ttgattgaat tctataatgc ggctgacggc gtgacggaga gctttcgacg
    35281 ggatggtgcc ccagtgggtg caaccgccgc caacattttg ataacgctcg ataactgcga
    35341 cgcgcacacc ttgcttaacc aggcccattg cagcgccttc gccgccgggg ccggaaccta
    35401 ttactatggc atcgtaatcg taggaatgtg gcatggtagg gcttacctgt tcttatacat
    35461 aaaagcaaca gaatggtaac attctatcgc gggtaagcca attgatcccc gtcatttatc
    35521 tggctatatc ctgagcggcc tttgctttgt ctgtttctta cttttgccct gacgttttat
    35581 tggattttta tcgacgatac tctccgttta agcggcaggt ttccgctgta cgtaaaagaa
    35641 ccggccagta aatatgtttt attgcgttac cgttcattca caatactgga gcaatccagt
    35701 atgttcattc tctggtatag tgccagcagt acttttggca aggattcaga catcgtgatg
    35761 ggcataagag cgcaacaaaa agaaaaaacc cgccgttcgc tggtggaagc cgcatttagc
    35821 caattaagtg ctgaacgcag cttcgccagc ctgagtttgc gtgaagtggc gcgtgaagcg
    35881 ggcattgctc ccacctcttt ttatcggcat ttccgcgacg tagacgaact gggtctgacc
    35941 atggttgatg agagcggttt aatgctacgc caactcatgc gccaggcgcg tcagcgtatc
    36001 gccaaaggcg ggagtgtgat ccgcacctcg gtctccacat ttatggagtt catcggtaat
    36061 aatcctaacg ccttccggtt attattgcgg gaacgctccg gcacctccgc tgcgtttcgt
    36121 gccgccgttg cgcgtgaaat tcagcacttc attgcggaac ttgcggacta tctggaactc
    36181 gaaaaccata tgccgcgtgc gtttactgaa gcgcaagccg aagcaatggt gacaattgtc
    36241 ttcagtgcgg gtgccgaggc gttggacgtc ggcgtcgaac aacgtcggca attagaagag
    36301 cgactggtac tgcaactgcg aatgatttcg aaaggggctt attactggta tcgccgtgaa
    36361 caagagaaaa ccgcaattat tccgggaaat gtgaaggacg agtaatgaaa caagcaaatc
    36421 aagatagagg tacgctgctg ctggcgttag ttgctggctt atcgattaat ggtactttcg
    36481 cagcgctgtt tagctccatt gtgccatttt ctgtattccc gattatttcc ctggtgctga
    36541 cggtttactg cctgcatcaa cgttatctta atcgcactat gccggtaggc ttgccgggtc
    36601 tggcagctgc ctgttttatt ctcggcgtac tgctgtacag cacggtagtt cgtgcggaat
    36661 atccggatat cggctctaac ttcttcccgg cagtactctc cgtcattatg gtgttctgga
    36721 ttggcgcgaa gatgcgtaac cgtaagcagg aagttgctga gtaatcggta ttatgccgga
    36781 tgctggcgca tccggcatgg gttttacttc gcggtcagta atacgccgca ctccatatgg
    36841 tgcgtgtagg ggaactgatc aaacagagcc agacgttcga ccttgtgcgt ctggcttaat
    36901 gtttccagat tcttgcataa cgtttccggg ttacaggaga tgtacaaaat acacggatac
    36961 gcctgcacca ttttctcggt ttcactgtcc agaccgctgc gcggtgggtc gacaaaaatg
    37021 gtttcgcact gataactctt taagtcgatc ccttgcaggc ggttaaactc gcgcacacca
    37081 ttcatcgcct gagtaaattc ttctgccgcc atacgaataa tttgtacgtt atcaatatgg
    37141 ttagctgcga tgttgtattg cgcagcagca accgacggct tagcgatttc ggtggctaat
    37201 acccgatcaa aattgcgcgc cagcgctaat gaaaagttac cgttgccgca gtacagctcc
    37261 agtaaatcgc cttttgagcc tttggttacg tccagcgccc attccagcat ctgaatattc
    37321 atcgccgcgt tcggctgggt aaagctgttt tctacctgac ggtagatcat ctctttccct
    37381 gcgaccggca gacgctcgtc gatgtaatct tgatccagct cgattttggt tttcgttgcc
    37441 cgaccaatca gatgcacatt cagattctgc gcgcgcagtg catcgcgcag ggcctccgct
    37501 tcctgacgcc actcatcatc cagcttctta tggtatagca gggaaaccac cgcctgatta
    37561 ctcagcgtgg tgaggtaatc aatctggaac aacttgtggc gcagaacggg attattacgc
    37621 acacccgcaa tcatcgccgt catcaactgg ttgataagtt cactggcggc ggggaagcta
    37681 tccacgcgga tacggctttt ggtttgttga tcgaaaatga tgtgatacag gtcatcgcca
    37741 tcgtgccaga tgcggaactc cgcacgcatc cggtaatgac tgaccggcga gcgaaacact
    37801 tccggaacca ggtcagaaaa cggtgccatc atactttgca aacgtaccac tttttcggct
    37861 aactgcgctt catactgttc tgttggaagg tgttcggggg tcatgatgta tcctgaaaaa
    37921 ttaaagtacg cggcgattgt agggattgct catcagatgt ccagatcttg atgaattcct
    37981 atttgtgagc tacgtctgga cagtaacttg ttacaacctg tagcatccac ttgtcggtcc
    38041 tgtgagttaa tagggaatcc agtgcgaatc tggagctgac gcgcagcggt aaggaaaggt
    38101 gcgatgattg cgttatgcgg acactgccat ccggtgggaa gtcatcatct cttagtatct
    38161 tagatacccc tccaagcccg aagacctgcc ggccaacgtc gcatctggtt ctcatcatcg
    38221 cgtaatattg atgaaacctg cggcatcctt cttctattgt ggatgcttta caatgattaa
    38281 aaaagcttcg ctgctgacgg cgtgttctgt cacagccttt tccgcttggg cacaggatac
    38341 cagcccggat actctcgtcg ttactgctaa ccgttttgaa cagccgcgca gcactgtgct
    38401 tgcaccaacc accgttgtga cccgtcagga tatcgaccgc tggcagtcga cctcggtcaa
    38461 tgatgtgctg cgccgtcttc cgggcgtcga tatcacccaa aacggcggtt caggtcagct
    38521 ctcatctatt tttattcgcg gtacaaatgc cagtcatgtg ttggtgttaa ttgatggcgt
    38581 acgcctgaat ctggcggggg tgagtggttc tgccgacctt agccagttcc ctattgcgct
    38641 tgtccagcgt gttgaatata tccgtgggcc gcgctccgct gtttatggtt ccgatgcaat
    38701 aggcggggtg gtgaatatca tcacgacgcg cgatgaaccc ggaacggaaa tttcagcagg
    38761 gtggggaagc aatagttatc agaactatga tgtctctacg cagcaacaac tgggggataa
    38821 gacacgggga acgctgttgg gcgattatgc ccatactcat ggttatgatg ttgttgccta
    38881 tggtaataca ggaacgcaag cgcagacaga taacgatggt tttttaagta aaacgcttta
    38941 tggcgcgctg gagcataact ttactgatgc ctggagcggc tttgtgcgcg gctatggcta
    39001 tgataaccgt accaattatg acgcgtatta ttctcccggt tcaccgttgc tcgatacccg
    39061 taaactctat agccaaagtt gggacgccgg gctgcgctat aacggcgaac tgattaaatc
    39121 acaactcatt accagctata gccatagcaa agattacaac tacgatcccc attatggtcg
    39181 ttatgattcg tcggcgacgc tcgatgagat gaagcaatac accgtccagt gggcaaacaa
    39241 tgtcatcgtt ggtcacggta gtattggtgc gggtgtcgac tggcagaaac agactacgac
    39301 gccgggtaca ggttatgttg aggatggata tgatcaacgt aataccggca tctatctgac
    39361 cgggctgcaa caagtcggcg attttacctt tgaaggcgca gcacgcagtg acgataactc
    39421 acagtttggt cgtcatggaa cctggcaaac cagcgccggt tgggaattca tcgaaggtta
    39481 tcgcttcatt gcttcctacg ggacatctta taaggcacca aatctggggc aactgtatgg
    39541 cttctacgga aatccgaatc tggacccgga gaaaagcaaa cagtgggaag gcgcgtttga
    39601 aggcttaacc gctggggtga actggcgtat ttccggatat cgtaacgatg tcagtgactt
    39661 gatcgattat gatgatcaca ccctgaaata ttacaacgaa gggaaagcgc ggattaaggg
    39721 cgtcgaggcg accgccaatt ttgataccgg accactgacg catactgtga gttatgatta
    39781 tgtcgatgcg cgcaatgcga ttaccgacac gccgttgtta cgccgtgcta aacagcaggt
    39841 gaaataccag ctcgactggc agttgtatga cttcgactgg ggtattactt atcagtattt
    39901 aggcactcgc tatgataagg attactcatc ttatccttat caaaccgtta aaatgggcgg
    39961 tgtgagcttg tgggatcttg cggttgcgta tccggtcacc tctcacctga cagttcgtgg
    40021 taaaatagcc aacctgttcg acaaagatta tgagacagtc tatggctacc aaactgcagg
    40081 acgggaatac accttgtctg gcagctacac cttctgaacc acgtcccacc gtgctggtgt
    40141 ttgactccgg cgtcggtggg ttgtcggtct atgacgagat ccggcatctc ttaccggatc
    40201 tccattacat ttatgctttc gataacgtcg ctttcccgta tggcgaaaaa agcgaagcgt
    40261 ttattgttga gcgagtggtg gcaattgtca ccgcggtgca agaacgttat ccccttgcgc
    40321 tggctgtggt cgcttgcaac actgccagta ccgtttcact tcctgcatta cgcgaaaagt
    40381 tcgacttccc ggttgttggt gtcgtgccgg cgattaaacc tgctgcacgt ctgacggcaa
    40441 atggcattgt cggattactg gcaacccgcg gaacagttaa acgttcttat actcatgagc
    40501 tgatcgcgcg tttcgctaat gaatgccaga tagaaatgct gggctcggca gagatggttg
    40561 agttggctga agcgaagcta catggcgaag atgtttctct ggatgcacta aaacgtatcc
    40621 tacgcccgtg gttaagaatg aaagagccgc cagataccgt tgtattgggt tgcacccatt
    40681 tccctctact acaagaagaa ctgttacaag tgctgccaga gggaacccgg ctggtggatt
    40741 ctggcgcagc gattgctcgc cgaacggcct ggttgttaga acatgaagcc ccggatgcaa
    40801 aatctgccga tgcgaatatt gccttttgta tggcaatgac gccagaagct gaacaattat
    40861 tgcccgtttt acagcgttac ggcttcgaaa cgctcgaaaa actggcagtt ttaggctgat
    40921 ttggttgaat gttgcgcggt cagaaaatta ttttaaattt cctcttgtca ggccggaata
    40981 actccctata atgcgccacc actgacacgg aacaacggca aacacgccgc cgggtcagcg
    41041 gggttctcct gagaatctca acagagaaaa gcaaa
//
LOCUS       DAJTYZ010000018        40839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000018 DAJTYZ010000000
VERSION     DAJTYZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.018"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            44..1708
                     /gene="asnB"
                     /locus_tag="OO596_000972"
     CDS             44..1708
                     /gene="asnB"
                     /locus_tag="OO596_000972"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6716898.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2088..2164
                     /locus_tag="OO596_000973"
     tRNA            2088..2164
                     /locus_tag="OO596_000973"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2122..2124,aa:Met,seq:cat)
     gene            2173..2257
                     /locus_tag="OO596_000974"
     tRNA            2173..2257
                     /locus_tag="OO596_000974"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2207..2209,aa:Leu,seq:tag)
     gene            2281..2355
                     /locus_tag="OO596_000975"
     tRNA            2281..2355
                     /locus_tag="OO596_000975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2313..2315,aa:Gln,seq:ttg)
     gene            2390..2464
                     /locus_tag="OO596_000976"
     tRNA            2390..2464
                     /locus_tag="OO596_000976"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2422..2424,aa:Gln,seq:ttg)
     gene            2480..2556
                     /locus_tag="OO596_000977"
     tRNA            2480..2556
                     /locus_tag="OO596_000977"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2514..2516,aa:Met,seq:cat)
     gene            2605..2679
                     /locus_tag="OO596_000978"
     tRNA            2605..2679
                     /locus_tag="OO596_000978"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2637..2639,aa:Gln,seq:ctg)
     gene            2716..2790
                     /locus_tag="OO596_000979"
     tRNA            2716..2790
                     /locus_tag="OO596_000979"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2748..2750,aa:Gln,seq:ctg)
     gene            complement(2944..4119)
                     /gene="ubiF"
                     /locus_tag="OO596_000980"
     CDS             complement(2944..4119)
                     /gene="ubiF"
                     /locus_tag="OO596_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6716899.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4265..5689
                     /gene="miaB"
                     /locus_tag="OO596_000981"
     CDS             4265..5689
                     /gene="miaB"
                     /locus_tag="OO596_000981"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6716900.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            5842..6882
                     /locus_tag="OO596_000982"
     CDS             5842..6882
                     /locus_tag="OO596_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6716901.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            6879..7346
                     /gene="ybeY"
                     /locus_tag="OO596_000983"
     CDS             6879..7346
                     /gene="ybeY"
                     /locus_tag="OO596_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6716902.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7436..8314
                     /gene="corC"
                     /locus_tag="OO596_000984"
     CDS             7436..8314
                     /gene="corC"
                     /locus_tag="OO596_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6716903.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8339..9877
                     /gene="lnt"
                     /locus_tag="OO596_000985"
     CDS             8339..9877
                     /gene="lnt"
                     /locus_tag="OO596_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6716904.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(9955..11832)
                     /locus_tag="OO596_000986"
     CDS             complement(9955..11832)
                     /locus_tag="OO596_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR6716905.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12287..13195
                     /gene="gltI"
                     /locus_tag="OO596_000987"
     CDS             12287..13195
                     /gene="gltI"
                     /locus_tag="OO596_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6716906.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13365..14105
                     /gene="gltJ"
                     /locus_tag="OO596_000988"
     CDS             13365..14105
                     /gene="gltJ"
                     /locus_tag="OO596_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6716907.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14105..14779
                     /gene="gltK"
                     /locus_tag="OO596_000989"
     CDS             14105..14779
                     /gene="gltK"
                     /locus_tag="OO596_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6716908.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14779..15504
                     /gene="gltL"
                     /locus_tag="OO596_000990"
     CDS             14779..15504
                     /gene="gltL"
                     /locus_tag="OO596_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6716909.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15622..16557
                     /gene="rihA"
                     /locus_tag="OO596_000991"
     CDS             15622..16557
                     /gene="rihA"
                     /locus_tag="OO596_000991"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6716910.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16649..18319
                     /gene="hscC"
                     /locus_tag="OO596_000992"
     CDS             16649..18319
                     /gene="hscC"
                     /locus_tag="OO596_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR6716911.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18379..19830)
                     /gene="djlC"
                     /locus_tag="OO596_000993"
     CDS             complement(18379..19830)
                     /gene="djlC"
                     /locus_tag="OO596_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6716912.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(19827..20534)
                     /locus_tag="OO596_000994"
     CDS             complement(19827..20534)
                     /locus_tag="OO596_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6716913.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20636..21190
                     /locus_tag="OO596_000995"
     CDS             20636..21190
                     /locus_tag="OO596_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6716914.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21200..22627)
                     /locus_tag="OO596_000996"
     CDS             complement(21200..22627)
                     /locus_tag="OO596_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6716915.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22624..23331)
                     /locus_tag="OO596_000997"
     CDS             complement(22624..23331)
                     /locus_tag="OO596_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6716916.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23495..24472
                     /locus_tag="OO596_000998"
     CDS             23495..24472
                     /locus_tag="OO596_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6716917.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(24542..25024)
                     /locus_tag="OO596_000999"
     CDS             complement(24542..25024)
                     /locus_tag="OO596_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6716918.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25259..27841
                     /gene="leuS"
                     /locus_tag="OO596_001000"
     CDS             25259..27841
                     /gene="leuS"
                     /locus_tag="OO596_001000"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6716919.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            27856..28437
                     /gene="lptE"
                     /locus_tag="OO596_001001"
     CDS             27856..28437
                     /gene="lptE"
                     /locus_tag="OO596_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6716920.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28437..29468
                     /gene="holA"
                     /locus_tag="OO596_001002"
     CDS             28437..29468
                     /gene="holA"
                     /locus_tag="OO596_001002"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6716921.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29470..30111
                     /gene="nadD"
                     /locus_tag="OO596_001003"
     CDS             29470..30111
                     /gene="nadD"
                     /locus_tag="OO596_001003"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6716922.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30135..30746
                     /locus_tag="OO596_001004"
                     /pseudo
     CDS             30135..30746
                     /locus_tag="OO596_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31006..31323
                     /gene="rsfS"
                     /locus_tag="OO596_001005"
                     /gene_synonym="rsfA"
     CDS             31006..31323
                     /gene="rsfS"
                     /locus_tag="OO596_001005"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6716923.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31327..31794
                     /gene="rlmH"
                     /locus_tag="OO596_001006"
     CDS             31327..31794
                     /gene="rlmH"
                     /locus_tag="OO596_001006"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6716924.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31825..33726
                     /gene="mrdA"
                     /locus_tag="OO596_001007"
     CDS             31825..33726
                     /gene="mrdA"
                     /locus_tag="OO596_001007"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6716925.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33729..34841
                     /gene="mrdB"
                     /locus_tag="OO596_001008"
                     /gene_synonym="rodA"
     CDS             33729..34841
                     /gene="mrdB"
                     /locus_tag="OO596_001008"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6716926.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            34852..35940
                     /gene="rlpA"
                     /locus_tag="OO596_001009"
     CDS             34852..35940
                     /gene="rlpA"
                     /locus_tag="OO596_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6716927.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDSVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36079..37290
                     /gene="dacA"
                     /locus_tag="OO596_001010"
     CDS             36079..37290
                     /gene="dacA"
                     /locus_tag="OO596_001010"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6716928.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37400..37663
                     /locus_tag="OO596_001011"
     CDS             37400..37663
                     /locus_tag="OO596_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6716929.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37764..38405
                     /gene="lipB"
                     /locus_tag="OO596_001012"
     CDS             37764..38405
                     /gene="lipB"
                     /locus_tag="OO596_001012"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6716930.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38665..39618
                     /locus_tag="OO596_001013"
     CDS             38665..39618
                     /locus_tag="OO596_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6716931.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            39827..40792
                     /gene="lipA"
                     /locus_tag="OO596_001014"
     CDS             39827..40792
                     /gene="lipA"
                     /locus_tag="OO596_001014"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6716932.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10244 a        10400 c        10276 g         9919 t
ORIGIN      
        1 gcaaacacaa caagcaacaa ataccaggtt aacggagaag gttatgtgtt caatttttgg
       61 cgtattcgat atcaaaacag acgcagttga gcagcgtaag aaagcactcg agctgtcacg
      121 cctgatgcgt catcgtggcc cggactggtc cggtatttat gccagcgata acgccattct
      181 cgctcacgaa cgtctgtcaa ttgttgacgt taacgcgggg gcgcaacctc tctacaacca
      241 acaaaaaacc cacgtactgg cggtaaacgg tgaaatctac aaccaccagg cattgcgcgc
      301 tgaatatggc gatcgttatc agttccagac agggtctgac tgtgaagtga tcctcgcgct
      361 gtatcaggaa aaagggccgg aatttctcga cgacttgcag ggcatgtttg cctttgccct
      421 gtacgacagc gaaaaagatg cctacctgat tggtcgcgac catctgggga tcatcccact
      481 gtatatgggg tatgacgaac acggtcagct gtatgtggcc tcagaaatga aatcgctggt
      541 gccagtttgc cgcacgatta aagagttccc ggcggggagc tatttgtgga gccaggacgg
      601 cgaaatccgt tcttattatc atcgcgactg gttcgacttt gatgcagtga aagataacgt
      661 gaccgacaaa aacgagctgc gtcaggctct ggaagattcc gttaaaagcc atctgatgtc
      721 tgatgtgcct tacggtgtgc tgctttctgg tggtctggat tcctcaatta tttccgctat
      781 caccaagaaa tacgcagccc gtcgcgtgga agatcaggaa cgctctgaag cctggtggcc
      841 ccagttacac tcctttgctg taggtctgcc gggttcaccg gatcttaaag cagcccagga
      901 agtggcaaac catctgggca cggtgcatca cgaaattcac ttcactgtac aggaaggtct
      961 ggatgccatc cgcgacgtga tttaccacat cgaaacttat gatgtgacca ctattcgcgc
     1021 ttcaacaccg atgtatttaa tgtcgcgtaa gatcaaggcg atgggcatta aaatggtgct
     1081 gtccggtgaa ggttctgatg aagtgtttgg cggttatctt tacttccata aagcgcccaa
     1141 cgctaaagaa ctgcatgaag agacggtgcg taaactgctg gccctgcata tgtatgactg
     1201 cgcgcgcgcc aacaaagcga tgtcagcctg gggcgtggaa gcacgcgttc cgttcctcga
     1261 caaaaaattc cttgatgtgg cgatgcgcat taacccgcag gataaaatgt gcggtaacgg
     1321 caaaatggaa aaacacatcc tgcgtgaatg ttttgagtca tacctgcccg caagcgtggc
     1381 ctggcggcag aaagagcagt tctccgatgg cgtcggttac agttggatcg acaccctgaa
     1441 agaagtggcg gcgcagcagg tttctgatca gcaactggaa actgcccgct tccgcttccc
     1501 gtacaacacg ccaacctcaa aagaagcgta tctgtaccgg gagatctttg aagaactgtt
     1561 cccgcttccg agcgccgctg agtgcgtgcc gggcggcccg tccgtcgcgt gttcttccgc
     1621 taaagcgatt gagtgggatg aggcgttcaa gaaaatggac gatccgtctg gtcgtgcggt
     1681 tggcgttcac cagtcggcgt ataagtaaga aatgcgaaac agccccggtg aaatccgggg
     1741 ctgtttcagt tattgaggtg cttgttgatt ctgatatgca gtaaggaaat gttccgggtt
     1801 tgccacatca ctgtttgaca actcatcact gtaggccagt atttcataga ttaatttccc
     1861 accaccattg ttgaattccg aatttcatcc tgtatgacaa aagaatggct gtagggtggg
     1921 gaaaaaacac aaagtaaaca ataattgacg aatatagcgc cacgctgttc gcaacctaac
     1981 caaacagtca ctttcgagca attttccttg aaaaagaggt tgacgctgca aggctctata
     2041 cgcataatgc gccccgcaac gccgataagg tatcgcgaaa aaaagatggc tacgtagctc
     2101 agttggttag agcacatcac tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc
     2161 accatctttt ttgcgggagt ggcgaaattg gtagacgcac cagatttagg ttctggcgcc
     2221 gcaaggtgtg cgagttcaag tctcgcctcc cgcaccattc accagaaagc gttgtacgga
     2281 tggggtatcg ccaagcggta aggcaccggt ttttgatacc ggcattccct ggttcgaatc
     2341 caggtacccc agccatcttc ttcgagtaag cggttcaccg accggttatt ggggtatcgc
     2401 caagcggtaa ggcaccggtt tttgataccg gcattccctg gttcgaatcc aggtacccca
     2461 gccatcgaag aaacaatctg gctacgtagc tcagttggtt agagcacatc actcataatg
     2521 atggggtcac aggttcgaat cccgtcgtag ccaccaaatt ctgaatgtgt cgaatacgtt
     2581 cggcaaattc aaaaaccaat ttgttggggt atcgccaagc ggtaaggcac cggattctga
     2641 ttccggcatt ccgaggttcg aatcctcgta ccccagccaa tttattcaag acgcttacat
     2701 cgtaagtgca ccagttgggg tatcgccaag cggtaaggca ccggattctg attccggcat
     2761 tccgaggttc gaatcctcgt accccagcca cattaaaaaa gctcgcttcg gcgagctttt
     2821 tgcttttctg cgttcattca atgtcgaatg cgatgttgac acgtcttatc cttcaatgtc
     2881 ggatgcgacg ctgccgcgtc ttatccgacc tacggttggc acgcatccgg caatgttgta
     2941 aggctacaac cctaacgcat atttcagcgc ctgacgtttc aacacgccag cacgctctgc
     3001 cgccattaac ccgagattac gcacaaaacg cagcggcggc agattattac tgaatccggc
     3061 ataaaacaga tccataccac tttgcataat gaagttatcc gccatgcgcc gcatctggta
     3121 acgcttgagg acaggataac tggcccacgc ttcaccgtag ctgcgggcat tcaccagaac
     3181 atcaatcagg gcatcgacat cacgataacc aagattcacc ccctgccccg ccagcggatg
     3241 gatggtatgc gcggcatcgc ccaccagcgc aagccccggc tgcacatact gcaacgcatg
     3301 tcggcgcgtc agcggaaacg caccagcggc aagcggtgta acgtaaccca gacgcgacgg
     3361 gaaatgcttc gcgatttccg cctggaattg cgccatattc atattctgca actggcgaat
     3421 acgcgccgga gagtcatacc acaccagcga tgcccagtta tcaaacaacg gcagaaacgc
     3481 acgcggtccg tccggggtaa attgctgcca ggtgctgtcg ccgggatcgt tctcacactg
     3541 tacgctaatc aacatgcacg actgcgcata ctgccaggca tgaacgccaa ttcccgccat
     3601 ctgccgcacc tgagaatttg cgccgtcggc accaatcacc agtttcgcgc ggatcgtttc
     3661 accgcccttc agttccagct cctgaagatc attatctcga tgtaatgcaa tcagcgagcc
     3721 tggcacacgt aacgttactt tcggatgcgc ttccagcgcc tgccacagcg cctgttgcag
     3781 gacggtgttt tccaccatat agccaagcaa cggtagctta agttcagcgg cgtcaaacac
     3841 cacatgcgcc gtttcccact cccacgtttc cagtctgcgg taaggatggc aacgcatagc
     3901 ctgtactgca tcccagaccc ctaacccttt aagcaatgat accgatgcgg cgctgatcgc
     3961 cgagatccgc acgtccggtt ggctatcagc gacaaacggt gcaggttctg cgtgctcgat
     4021 caccgttacc gaaaatccgt gctgtgccag ccccagcgcc agcgcgccgc cgaccattcc
     4081 tccgccgaca atggcaattt ccgttggttg atttgtcatg gtcaatcatc ctgttagcaa
     4141 tatgctttaa gtttacagga tttttacccc caagggctga taacgctcat actggtcaca
     4201 acggcagcaa agcattacac tatacgccct gcattcctgg ctactatttc gcaagagcaa
     4261 gtcgatgacc aaaaaactcc atattaaaac ctggggctgt cagatgaacg agtacgattc
     4321 atcgaagatg gccgatctgc tggatgccac ccacggctat caactgaccg acgtggcgga
     4381 agaagcggat gtgctgctgc tgaacacctg ctcaatccgc gagaaggctc aggaaaaagt
     4441 cttccatcag ttgggtcgct ggaaactgtt aaaagagaag aatccagacc tgattatcgg
     4501 cgtcggtggc tgcgtggcat cgcaagaagg cgagcacatt cgccagcgcg cccactatgt
     4561 cgatattatt tttgggccgc aaacgctgca ccgtctgccg gagatgatca actccgtgcg
     4621 cggcgaccgc agcccggttg tagatatcag cttcccggaa atcgagaagt ttgaccgtct
     4681 accggaaccg cgcgccgaag ggccgaccgc gtttgtctcc atcatggaag gctgcaataa
     4741 atattgcacc tactgcgtgg tgccttacac ccgtggtgaa gaggtaagcc gtccgtctga
     4801 cgatattctg tttgagattg cccagcttgc ggctcagggc gtgcgtgaag tcaacctgct
     4861 cggtcagaac gtgaacgcct ggcgtggtga aaactacgac ggctccaccg gatcgtttgc
     4921 cgatctgctg cgtctggttg ctgcgatcga cgggatcgat cgtattcgct ttaccaccag
     4981 ccatccgatc gaattcaccg acgatatcat cgaagtgtat cgcgacacgc cggagctggt
     5041 gagcttcctg catctgccag tacaaagcgg ttccgatcgc attctgaacc tgatgggccg
     5101 tactcacacg gcgctggagt acaaagcgat catccgtaaa ctgcgtgcgg cgcgtccgga
     5161 tattcagatc agttctgact tcattgttgg cttccctggc gaaaccaccg aagacttcga
     5221 gaaaacgatg aagctgattg ccgacgtcaa tttcgacatg agctacagct ttatcttctc
     5281 tgcacgtccg ggtacaccag ccgccgatat ggttgatgat gttccggaag aagagaagaa
     5341 gcaacgtctg tatattctgc aagaacgtat caatcagcaa gcgatggcat ggagccgccg
     5401 gatgctcggc accacccagc gtattctggt agaagggact tcgcgtaaga gcatcatgga
     5461 gctttccggg cgtaccgaaa ataaccgcgt ggtcaacttc gagggcacac cggatatgat
     5521 cggtaaattc gtcgatgtag aaattaccga cgtctacccg aactctctgc gcggtaaagt
     5581 ggtgcgtact gaagatgaaa tgggtctgcg cgtggcagaa acaccggaat cggtgattgc
     5641 ccgtacccgc aaagaaaacg accttggcgt gggttattat cagccgtaat tctcaggccc
     5701 gccgttccgg tgggccttgt tttttctctc tttatcccca tctttatcgc aatgcttgcg
     5761 cctttttcct gtggcgtgaa taatttcctt ataggccggt gaatgttcag cttccggcgg
     5821 catacagata agaggaacgg tttgaacata gacactcgcg aaatcaccct ggagccagca
     5881 gacaacgcgc gtctgttgag cctgtgcggc ccgtttgatg acaacatcaa gcagctcgaa
     5941 cgccgtctcg gcatcgagat caatcgccgc gacaaccact ttaaactgac cggccgtccg
     6001 atttgcgtca ccgctgcggc agacattctg cgtagcctgt atgtcgatac agccccgatg
     6061 cgcggtcaga ttcaggatat cgaaccggaa cagatccacc ttgcgattaa agaagcgcga
     6121 gtactggagc aaagtgcgga gagcgtgccg gagtacggca aagcggtcaa tatcaaaacc
     6181 aaacgcggcg taattaagcc gcgcacgcca aaccaggcgc agtacatcgc caatattctc
     6241 gaccatgaca ttaccttcgg cgttggcccg gcgggtacgg gtaaaaccta cctggcagtg
     6301 gctgcggcag ttgatgccct ggagcgtcag gagattcgcc gtattctgct gactcgtccg
     6361 gcggtcgaag ccggtgagaa actgggcttc ctgcctggcg atttaagcca gaaagtagac
     6421 ccgtatctgc gcccactgta cgacgcgctg tttgaaatgc tgggctttga gaaagttgag
     6481 aaactgattg agcgcaacgt tattgaagtc gcgccgctgg cctatatgcg tggtcgtacg
     6541 ctgaacgacg cgtttatcat tctcgatgag agccagaaca ccaccatcga acagatgaag
     6601 atgttcctga cccgtatcgg ttttaactca aaagcggtta tcaccggcga cgtcacacag
     6661 atcgacctgc cgcgtaatac taaatcaggc ttacgtcacg ccatcgaagt gctggccgat
     6721 gtcgaagaga tcagctttaa cttcttccac agcgaagacg tggttcgtca ccctgtggtg
     6781 gcgcgtatcg ttaacgccta tgaagcctgg gaagaagccg aacaaaaacg aaaagcggcg
     6841 ctggcggcag aacgcaagcg cgaagaacag gaacaaaaat gagtcaggtg atcctcgatt
     6901 tacaactggc atgtgaagat aattccgggt taccggaaga gagccagttt caaacatggc
     6961 tgaatgcggt gatcccgcag tttcaggaag aatcggaagt gactattcgc gtggtcgata
     7021 ccgccgaaag ccacagtctg aatctgacct atcgcggtaa ggataagccg accaacgtgc
     7081 tctccttccc gtttgaagtg ccgcctggca tggaaatgtc gctactgggc gatctggtta
     7141 tctgccgtca ggtggttgag aaggaagctc aggagcaagg caaaccactg gaggcgcact
     7201 gggcgcatat ggtggtgcac ggcagtctgc atttgttagg ttacgatcac atcgaagatg
     7261 acgaagcaga agaaatggaa gccctcgaaa cagagattat gcttgctctg ggctatgagg
     7321 atccgtacat tgccgagaaa gaataatttg ccaggcattt gactggcaga cgtccccgcc
     7381 gttggtgatt aacgacgacg ttttgattaa ctaacatgag aacccatacg acgccatgag
     7441 cgacgacaat tcacacagta gtgacacgat aagcaacaag aagggatttt tctccctgtt
     7501 actcagccaa cttttccacg gtgaaccgaa aaaccgtgac gaactgctgg cgctgatccg
     7561 tgattccggg cagaacgacc ttatcgacga agatacgcgc gatatgctcg aaggggtgat
     7621 ggacatcgca gaccaacgcg ttcgcgacat catgatcccc cgctcccaga tgattaccct
     7681 gaaacgcaac cagacgctgg acgaatgcct tgatgtcatc atcgagtccg cccactcacg
     7741 tttcccggtg attagcgaag acaaagatca cattgaaggg attctgatgg cgaaagactt
     7801 gctgccgttt atgcgcagcg atgctgaagc cttcagcatg gacaaagtgt tacgtcaggc
     7861 ggttgtcgtt cctgaaagta agcgcgtaga ccggatgctg aaagagtttc gctctcagcg
     7921 ttaccacatg gcgatcgtca ttgacgaatt cggtggggtt tccggtctgg tgaccattga
     7981 agacatcctg gaactgattg ttggtgagat tgaagacgag tatgacgaag aagatgatat
     8041 cgacttccgt cagctgagtc gtcatacctg gaccgtgcgt gcactggctt ccattgaaga
     8101 cttcaacgaa gcgttcggca cccactttag cgatgaagag gttgacacta tcggtggtct
     8161 ggtgatgcag gcatttgggc atcttccggc gcgtggcgaa actatcgaca tcgacggtta
     8221 ccagttcaaa gtggcgatgg ccgacagtcg gcgtattatt caggttcatg tcaaaatccc
     8281 ggatgactca ccccagccga agctggatga ataaaaccga aactggatag ataactacat
     8341 ggcttttgcc tcattaattg aacgccagcg cattcgcctg ctgctggcgt tattattcgg
     8401 tgcctgcgga acgctggcct tctctcctta cgacgtctgg cctgcggcga ttatttcgtt
     8461 gatggggctt caggcgttga cctttaaccg ccgtccactc cagtctgccg ctattggctt
     8521 ttgctgggga tttggcctct ttggcagcgg tattaactgg gtctatgtca gcatcgcgac
     8581 ctttggcgga atgcctggcc cggttaacat cttcctggtg gtgctgctgg cggcgtattt
     8641 gtcgctgtat accggactgt ttgctggcgt gctgtcgcgt ctgtggccga aaaccacctg
     8701 gctgcgcgtg gcgattgccg cccctgctct ctggcaagtg accgagtttc tgcgcggttg
     8761 ggtactgaca ggcttcccgt ggttacagtt cggctatagc cagattgatg gcccgttaaa
     8821 agggctggca ccgataatgg gcgtggaagc cattaacttc ctgctgatga tggttagtgg
     8881 cctgctggca ctggcgttgg tcaaacgcaa ctggcgtccg ctggtggtgg ccgtcgtgct
     8941 gtttgcccta cccttcccgc tgcgttacat ccaatggttt accccgcaac cggagaaaac
     9001 cattcaggtt tcgatggttc agggcgatat tccgcaatca ctgaaatggg atggagatca
     9061 gcttcttaat acgctgaaga tttactacaa cgcaacggct ccgctgatgg gcaaatcatc
     9121 gttgattatc tggccggagt cggcgataac cgatctggaa attaatcagc aaccgttcct
     9181 caaagcactg gacggtgagt tgcgcgataa aggtagctcg ctggtaaccg ggattgtcga
     9241 cgcgcgtctc aataagcaga accgctacga tacctacaac accatcatca cactgggtaa
     9301 aggtgcacca tacagctacg aatcagccga tcgctataac aaaaaccatc tagtgccgtt
     9361 tggcgagttt gtcccgctgg agtcgattct gcgtccgtta gcaccgttct ttgatctgcc
     9421 gatgtcgtcg ttcagccgtg ggccatatat ccagccgccg ctgtcggtaa atggtattga
     9481 gcttactgcg gctatttgct acgagatcat tctcggcgag caagtgcgcg ataacttccg
     9541 cccggatacc gactatctgc tgactatctc caacgatgcg tggtttggta agtctattgg
     9601 tccatggcag cacttccaga tggcgcgaat gcgtgcgctg gagctggcgc gcccactgtt
     9661 gcgcagcacc aacaacggca ttacggcggt gattggcccg cagggggagg ttcaggcgat
     9721 gatcccgcag ttcacccgcg aggtgttgac cactaatgtg acaccgacca ccggactcac
     9781 accgtatgca cgtaccggca actggccact gtgggtgctg acggcgctgt ttggttttgc
     9841 cgctgtgttg atgagtctgc gagcacgtaa acgttgatgt gcctgatgta tcctgcatga
     9901 gcaaaacgta ctttctcatt gagctttggg gcatggttat catgcccctt tttcttacgg
     9961 ttcagggata caagccacat tgccatattg gggatgacga ataattgcct gcggatcaag
    10021 cgagcgcgtc ctgtccattt ggatcaaccc gttcttcgcc atgacaaata tttttctacc
    10081 atccgctgaa aaacgaaggt gattaacgta gctggcagga taaacgccaa taatctgccc
    10141 ggcattaata ttgagcagga tgacataacc ttgcaggcca tatgacacca acgctaaata
    10201 ttgaccgtcc gggctaatac cctcaacgga ataaacgctc ctgctacacc attgcgtcat
    10261 aatgctggct tgttgatccc ctacgcttat acgtgaaatc ccctgactat cggcaaaaat
    10321 aacctcgccg ttttcaggtc gaaaacgcat cgccgatatc gttccacgcg catcacctgc
    10381 gggaaagtct tctttctgat gcgtgatggt attgatccgc acgatgttac ctctgcctgc
    10441 tcccggaacc agtaatgttc tttcatccgg ggaaagtgcc ataacccagg cgcctggttt
    10501 accgctcgtc cccgtcccgt cgtatttctc cagcgggata tccgccacat gctgataagt
    10561 caccagatca atcgctggca cactattact ggtgcgattc atcacccagg cttttgtgcc
    10621 atcacgcgag aggataaggc tgtcaggatg tgcaccggtg gtaatcgact gaataatctc
    10681 ccctgtcgcc acgttaatga ccgataacgc attcgcttca aatgaagtcg cataaatcaa
    10741 cttttcatcc gggcttaatg ccagcgatct gacgccctga tctttacagc caccacaatg
    10801 tttgactgac ttttctttcg ctatgggcgc tgtaaaggta tgcaggattt tcttttgtcc
    10861 gaggtcgaaa acaacaatgc tgttcgtgtt ttcaattgcg gcatacaaca tattaccatc
    10921 ccgactgatc gccatatcat ggatatcggc aatctcagcg agcaaatcac cactggcctg
    10981 ttcacgactt accggagcgg ataatgtttc cctgagggcg ttgcgttcct ccgctaactg
    11041 ctgctttttt tgccgtcgct cttgttcaat ctcctgtgga taaaacgctt cacgcaggct
    11101 ttgcctgacc tgtaaaactg actcatccat ctgctgctgc gcaagccaga tagccccggt
    11161 cagaagaaga ctgcccgcaa caataatccc gccttccgta acgagtcggt tttgcccaga
    11221 taaacgcgcg ctcagccaac ccaacgcgcc acccgcaatc aggccgccaa tgtggcaagc
    11281 gttatctatt cctgtttgcc gggcaccgta caacagtgtc agcgccacca tacccaacag
    11341 aggaaatact cgccgctggt ttttatggta tgtgcccgta cctgaagcaa actgtgttgc
    11401 tatcgctgca ccagctattc ccagaatcgc cccggatgcc ccaacgctga tgactatctg
    11461 ctcactttcg cgtaacgcat aacaggcgct gatgagccca ccaaagacgc cggagaataa
    11521 ccaggtaatc aggagtcgcc atttccccag aatctgttcc gccacgcctc ccacgaccag
    11581 gaatgcaaac atattcatca gcagatgaga aacactactg tgcagaaaga tactgctcaa
    11641 taagcgccag ggttcacccg acaaagtaag aggcgcaacg tttgcgccga gatggataag
    11701 tgcggattga tcgacctctt cataactcat ccattgtgcg agataacaaa atatcccaat
    11761 acagagtgca aaaaggataa gggttattgc gggtaattga acatttaacg gcttaaccga
    11821 agatgcagac ataaggtgtt tcttccttga aaagacaggt aaaaagcatt tttcccgtgt
    11881 gcagttatca tcccggcaaa cgtcccatgc cgaaaagata attctgtccg aaatatgact
    11941 ttttcagcct aaaattacaa tcgtaattaa ttcgccccta agtatttttt ggcatgtaag
    12001 ttgctttatt tactgtgtaa gcgatgaaat cagccgcgac gcaaccaggt tgcaccacca
    12061 caaatcagcg gtagcaccaa aaaggtgcgg tttaagaatt ttgcttcgga atggtgcggc
    12121 gcatctcgca aaaacaaaca ataaaacagc aatatcttta catttagcca aactaaatgt
    12181 tacctaacag gcacaacact gcacaataaa gttgcagacg ataacaacac aaacactcac
    12241 aacgggtatc catgcgttct taacgcagaa gataaaggag ttggatatgc aattacgtaa
    12301 acctgccaca gcaatcctcg ccctggcgct ttccgcagga ctggcacagg cagatgacgc
    12361 cgccccggca gcaggcagta cgctggacaa aatcgccaaa aacggtgtga ttgtcgtcgg
    12421 tcaccgtgaa tcttcagtac ctttctctta ttacgacaat cagcaaaaag tggtgggtta
    12481 ctcgcaggat tactccaacg ccattgttga agcagtgaaa aagaaactca acaaaccgga
    12541 cttgcaggta aaactgattc cgattacctc acaaaaccgt attccactgc tgcaaaacgg
    12601 cactttcgat tttgaatgtg gttctaccac caacaacgtc gaacgccaaa aacaggcggc
    12661 tttctctgat actattttcg tggtcggtac gcgcctgctg accaaaaagg gcggcgatat
    12721 caaagatttt gccgacctga aagacaaagc cgtagtcgtc acttccggca ctacctctga
    12781 agttttgctc aacaaactga atgaagagca aaaaatgaat atgcgcatca tcagcgccaa
    12841 agatcacagt gactctttcc gcaccctgga aagcggtcgt gccgttgcct ttatgatgga
    12901 tgatgccctg ctggccggtg aacgtgcgaa agcgaagaaa ccagacaact gggaaatcgt
    12961 cggcaagccg cagtctcagg aagcctacgg ttgcatgttg cgtaaagacg atccgcagtt
    13021 caaaaagctg atggatgaca ccaccgcgca ggtgcagacc tccggtgaag cggaaaaatg
    13081 gtttgataaa tggttcaaaa atcccattcc gccgaaaaac ctgaacatga atttcgaact
    13141 gtcagacgaa atgaaagcac tgttcaaaga accgaatgac aaggcactga actaattaca
    13201 agaaccaggg gcggaaattc cagccctctc gattgttacg tagcacggac agactatacg
    13261 cctgatggtc gttccccatc gggcctgaaa accgcaatac gctgggtaac aatcttcgag
    13321 ggtagcagtt aacgctgcta ccctcttttt tctggagtag atttatgtct atagactgga
    13381 actggggtat ttttttacaa caagccccgt tcggcaacac cacctatctt ggttggatct
    13441 ggagtggttt tcaggtgacg atcgctttgt cgatctgcgc ctggattatc gctttcctcg
    13501 tcggttcatt tttcggcatt ttacgtaccg ttccaaaccg ttttctctcc ggtctgggta
    13561 cgttgtatgt cgaactgttc cgcaacgtgc cgttgattgt gcaattcttt acctggtatc
    13621 tggtgatccc ggagctgttg ccggagaaaa tcggcatgtg gtttaaggcc gagctggatc
    13681 ccaatattca gtttttcctt tcatccatgc tctgcctggg gctgtttacc gccgcccgtg
    13741 tttgcgaaca ggtacgcgcc gccattcagt cgttgccgcg tgggcaaaaa aatgccgcgc
    13801 tggcgatggg cctgacgcta ccgcaggctt atcgatacgt actgctgcct aatgcttatc
    13861 gcgttatcgt cccgccgatg acttccgaga tgatgaacct ggtgaaaaac tccgccatcg
    13921 cctcgactat tggtctggtg gatatggcgg cgcaggcggg taaattgctt gattactcag
    13981 cccacgcctg ggagtcgttt accgctatta cgctggcgta tgtgttgatt aacgcggtta
    14041 tcatgctggt aatgacgctg gttgaacgta aagttcgcct gcctggcaac atggggggca
    14101 aataatgtac gagtttgact ggagttccat tgtcccttcc ctgccatatc tgctcgacgg
    14161 gctggtgatc acgctgaaaa tcaccgtcac ggcagtcgtg attggtattt tgtggggcac
    14221 catgctggcg gtgatgcgct tgtccagctt tgcgcccgta gcgtggtttg ccaaagccta
    14281 cgttaacgta ttccgctcga ttcctttagt tatggttttg ctgtggtttt acctgatcgt
    14341 gccaggtttt ctgcaaaacg tgctgggatt atcaccaaaa aatgatatcc gtttaatttc
    14401 ggcgatggtg gcgttttcga tgtttgaagc ggcctactat tcagagatta tccgtgctgg
    14461 tattcaaagt atttctcgtg gtcagtcgag cgccgcgctg gcgttgggaa tgactcactg
    14521 gcagtcgatg aaactgatta ttctgccgca ggcgttccgc gcgatggtgc cgcttctgct
    14581 cactcagggc atcgtactgt tccaggatac ctcactggtg tatgtgttat ctctggccga
    14641 tttcttccgt accgcctcaa ccattggtga gcgtgatggt acgcaggttg agatgatcct
    14701 gtttgccgga tttgtttatt tcgttattag tcttagcgcg tcgttgttgg tcagctactt
    14761 gaaaagaagg acagcataat gattaccctg aaaaatgttt caaaatggta tggtcacttt
    14821 caggtgctga ccgactgctc aaccgaagtg aaaaaaggcg aagtggtggt ggtttgcggc
    14881 ccgtctggtt ccggcaaatc aacgctgatt aaaaccgtca acggcctcga accagtgcag
    14941 caaggtgaaa tcaccgtcga tggtatcgtg gttaatgaca agaaaaccga tctggcaaag
    15001 ctgcgttccc gcgtcgggat ggtattccag catttcgagc tgttccctca tctgtcgatt
    15061 atcgaaaacc tgaccctggc gcaggtgaaa gtgcttaaac gcgataaagc gcccgctcgt
    15121 gaaaaagcat tgaaattgct ggagcgcgtg ggactttccg cacatgccaa taaatttccg
    15181 gcgcagcttt ccggcggtca gcagcagcgt gtggcaatcg ctcgcgcatt gtgtatggat
    15241 cctattgcga tgctgttcga cgaaccgaca tcggcgctgg atccggagat gatcaacgaa
    15301 gtgctggacg tgatggtgga actggcgaac gaaggaatga ccatgatggt ggtgacccac
    15361 gaaatgggct ttgcccgcaa agtggcgaat cgggtgatct ttatggacga gggtaaaatt
    15421 gtcgaagact cgccgaaaga cgctttcttc gatgatccga aatcggaccg cgcgaaagac
    15481 ttcctcgcga aaatcctgca ttaatcactc tggcgcgcat acccaagtgc gcgccatctc
    15541 acgctttatc gtttcgcccc tcgtatctgt tacatcctgg cgttaccctt gtcgcaaaga
    15601 agcacacaac aaggagcaac aatggcactg ccaattctgt tagattgcga cccaggtcat
    15661 gacgacgcta tcgcaatagt tctcgccctc gcctcaccag agcttgatgt caaagcaatt
    15721 acgtcttccg ccggaaacca gacaccagaa aaaaccttac gcaatgttct gcgtatgctg
    15781 accttgctta atcgcaccga tattccggta gcaggcggcg cggtaaaacc gttaatgcgt
    15841 gagttgatta tcgcggacaa tgtgcacggc gaaagcggtc tggatggccc ggcattaccg
    15901 gaaccgacgt tcgcaccgca aaactgtacg gcggtagagc tgatggcgaa aacgctgcgc
    15961 gaaagtgcgg aacctgtcac gattgtgtct accggaccgc aaactaacgt tgccttgctg
    16021 ctcaatagcc atccggaact gcatagcaaa attgcccgta tcgtgattat gggcggcgca
    16081 atggggcttg gtaactggac gcctgcggct gaatttaata tttacgttga cccggaagcg
    16141 gcagaaattg tcttccagtc agggatccca gtggtgatgg ccggtctgga tgttactcac
    16201 aaagcacaaa tccacgttga agacaccgag cgtttccgcg cgattggcaa ccctgtttca
    16261 accattgttg ccgaactgct ggatttcttc ctcgaatatc ataaagacga aaaatggggc
    16321 tttgtcggtg caccgctgca tgacccatgc accatcgcct ggctgttgaa acccgagttg
    16381 tttaccactg ttgagcgctg ggttggcgtg gaaacacagg ggaaatatag ccagggtatg
    16441 acggttgttg attattatta tctgactggc aataaaccga atgccactgt aatggtcgat
    16501 gttgatcgtc agggctttgt tgatttactg gctgatcgtc tgaaatttta cgcttaacac
    16561 acgtcattga ttaagatcac cacgcgttac ctgggtggtc ttaatctgtt tatctgttta
    16621 tctctttttc tttcaagata taaatgcaat ggataatgca gaactcgcca ttggtatcga
    16681 tctcggcact accaatagtt taattgccgt ctggaaagac ggtgccgcgc aattaattcc
    16741 aaataagttc ggtgaatatt taacaccatc cataattagc atggatgaaa ataatcatat
    16801 tttagtcgga aaaccggctg tatcacggcg tacttcgcat ccggataaaa cggcagcgtt
    16861 atttaaacgt gcaatgggca gtaataccaa ctggcggtta ggcagcgaca catttaacgc
    16921 gccagaactg tcctctttgg tattacgctc attaaaagaa gatgccgaag aatttctgca
    16981 acgtccgatt aaagatgtgg tgatctccgt tccggcttat ttcagcgatg aacaacgcaa
    17041 gcatacccgt ttagcagcgg agttagccgg gttaaatgcg gtacgcttaa ttaatgaacc
    17101 cacagcagct gcgatggcat atggcctgca tacgcaacaa aatacccgct cgctggtttt
    17161 tgatctcggt ggcggcacgt ttgacgtcac ggtgctcgaa tacgccacgc cggtgattga
    17221 agttcacgcc tccgctggcg ataacttcct cggtggcgaa gattttaccc atatgttggt
    17281 cgatgacgtt ttaaagcgcg cggatgtcgc caggaccacg cttaacgaga gtgaactggc
    17341 agccttgtac gcctgtgtgg aagcggcaaa atgtagcaat caatcgccat tgcacattcg
    17401 ctggcagtac caggatgaaa cgcgggagtg cgaattttac gagaacgaac tggaagattt
    17461 gtggttgccg ctgctcaatc gcttgcgagt gccgattgaa caggcgttgc gcgatgcacg
    17521 tctgaagccg agtcaaatcg acagtctggt gctggttggc ggcgcgtcac aaatgccgct
    17581 ggtgcagcga atcgccgtgc gtctgtttgg caaattaccg tatcaaagtt acgacccgag
    17641 caccattgtc gcgctgggcg cagcaatcca ggccgcctgc cgcttacgca gtgaagatat
    17701 tgaagaggtg atcctcactg atatttgccc ttactcgttg ggcgttgaag ttaaccgcca
    17761 gggcgtttcc ggcattttct cgccaattat tgaacgaaac accacagtac ccgtgtcgcg
    17821 tgtagaaact tactcgacca tgcacccgga acaggattct attacggtta acgtctatca
    17881 gggagaaaac cacaaagtta aaaataacat tctggttgaa tccttcgatg tgccgttgaa
    17941 gaaaaccggg gcttatcagt cgattgatat tcgctttagt tatgatatca acggcttgct
    18001 tgaagttgac gtgcttctgg aagatggcag cgttaagtcc agagtcatta atcatagccc
    18061 ggtaacattg agcgcgcagc agattgaaga gagtcggacg cggttatccg cattgaaaat
    18121 ttatccgcgc gatatgctaa tcaaccgcac ctttaaggcc aaactggaag agttgtgggc
    18181 gcgggcgctg ggtgacgagc gagaagagat tggccgggtg atcaccgatt ttgatgcggc
    18241 gttgcagtca aacgatatgg cccgcgtcga tgaagttcgt cgacgggcga gcgattattt
    18301 ggctattgag atcccttaaa cacggctgcc ggatgcggca taaacgctgc atccggcatc
    18361 ggcgcaaaaa acacatcctc agctaaacaa catcgcgatt tttccgagcg cataacctat
    18421 catcagcacc gttaagctga ttttgataat actggtaatg ctctgtggtt tttcatcctc
    18481 atcatttttt tcttcatggt tttcaggtgt tggctcatcg acatcttcat cctccggctc
    18541 gagtttcact ttcgtcaaca gcatctgggt gataatggcc tctaatggat gagcatctcc
    18601 accttccagc cttgtattaa gacgggccag catctcttgt ttcgggaacg cttccagcac
    18661 aacctgttcc gctacccgat acaaatccag taacggtgaa tcttgctctg tctcgctacc
    18721 gactctcgat ttccaccacg cccatgcttt gcgcttgaag aataacgact caataaacga
    18781 ttgacctaac ggcgataatt ttccgctatg taaaatctcc gcccataaca gccgcgtccg
    18841 ggcgctctca ccaggaacat atttcaacgg tttaccttgc gcatctacca tgcttcgcgc
    18901 ttcgataacc atcactaata acgcgaaata atccgggcaa tgacgcttgc tccactgcaa
    18961 caacaaatta tcagcttgtg tagatgggta actttcccag tacgcgcaga gatcggcaag
    19021 caggctttcc ccctcgccgc agtacaaacg ttgtgcacac tggtaatacc gggcatcttc
    19081 gctttctggt tcttccgctt gccactgttt ggctacagga atgagttccg ccatacctgt
    19141 ttgcaccgag ctgtaccagc gcaacagttt gcgatgtaac tttgcatcat caggaatgaa
    19201 ccacggtcct tccatcgcca aaaacgcagt gacatagtca ggatggtaac ggcagcaacg
    19261 ttccagcgcg aagtaatatt caacagtctg gttctgtaac gcgacgggat attccagaag
    19321 gctaaggaaa tcgaaacaat caccagcctg aatggcctca agaaaagcct ccctttcttc
    19381 ctcatccatt ccttcgctgt cattaaacga atgccagttc aaacgctctg ccaacaaaga
    19441 agcacaattt aatgaaagat atcgcgcttc tatagcgatt gcacacagcg gccagcgtaa
    19501 ttgatcgaca tcctccatgt tccaggtatt taattgctga ataaatttct gccaggcgga
    19561 gggctgaaaa cgatcacttt ctgaatccag taatgtcctg aatgcacgta gtatttcatg
    19621 ttcagcggtg gcatcttttt cttcatcatc ggcttcctcg acaggattta ccgccagtcg
    19681 cagcgcctct tcataggcct ggcgtaatgc tttaaacccc tgcggatcgg tttcgggatg
    19741 acacaacggt aagcgagcaa gataagcctg gcggataatg tctatttgcg tcgtgctttc
    19801 aatttcgagg atttgccaac atgttttcat aggtcgctca catccttaag cgttaccggg
    19861 cattcaggct ggacagttaa tggttgccag ggaagcgtgg catagaaacg gtcgcttccc
    19921 gctgccagct cttcgaacat ccgactcccg gcgctatcgt atttgtacag ggttagcgcc
    19981 tcgcgtagcg aagcgttgtc ttcgcactga gaagattgcc agtagagacg ccctagtgaa
    20041 taggcagaaa aatagtgcat ccagctgtga tagtaatggt gagctcgtgc atagacgcgc
    20101 gattgcagcc ataaactctc ttcttccgtc agccagttgt tgagcacacc gacccgtgac
    20161 agaaacccca ttcgaacata atcccaggct ttgattcccc cttctccaca gcacatagcg
    20221 gtttgggcaa cgaattgggt ataaatcaac cctctctccg agccaccagc aattaatgct
    20281 ttcctctctt caggggaaga gcgttgccat gtcagatata agccagctaa atcattggca
    20341 tgaccatcat cagtcatctg ataaatagtc tgaattaaat catcacgatt atttattccc
    20401 caggatgatg agagatcgaa ttcttttatc ccagtataca tttttggcga gtcatgcgcg
    20461 ggtttgcaac tctgattaag tatttccatg ggcgcagaaa gcgcaaaaag aagatattgt
    20521 tcttccttat tcatttttta ataatccctt aagtgcatta actgactact tttttattat
    20581 tgatgagtat aaacttgacg ctttcaaaat aaaaagaaaa tcgaagcatt cacacatgaa
    20641 taaaaaatca atgtatatat tcgcaatttt tatagttgca gcaattacct gtattagcca
    20701 acccaagaaa acgacgttgc gtgataaagc catggtgaat tatgcctttg attatttaag
    20761 atcaccaggc agtctaccgt taaccacggc agccacggag ctttccgcga ttcatggtca
    20821 ctcaacatcg caatatcgcc ttggcgaatt ttatcttcat ggtagcgacg gtaaaccact
    20881 ggattataca caggcgagat actggtatga gcaatcagca gaacaggaaa atccgcgcgc
    20941 acaaagcaaa ctggggtgga tctacctgaa aggcctggga gtcaaacccg atacccgtaa
    21001 agcaattctc tggtataagg aagcagctga acaagggtat gctcatgctc aatatacttt
    21061 gggtttgatc tacagaaatg gcactggtat taatgttaac cattatgaat ctcaaaaatg
    21121 gttaaaactg gccgccaaac aacattacaa aaatgcggaa agattacttg ccggacttcc
    21181 cgcacattaa cgtcgactgt cacccgaaga gatggagtat ttttcctatc aaaccggcaa
    21241 agataaaaat gtagaaaata attttgatga cgcccagaca gcccagcccg ccatcgtcaa
    21301 cgggcattgg ctgcggttgc ggctccggct cgacggtttc ctgaccggta aaattcgccg
    21361 cctgaattaa taatcgacga ataattaatg cttccagtgg acaacgatct tctgcatcaa
    21421 ggcggctcac aatcggcatt aatgattctt ctgtcggttg gctggcctgc actaattgtt
    21481 tggcaactaa atataatggc cataagggat gttctgtatc acgcgtgagt gagatccgcg
    21541 gcttacgtcc tttgtgcaaa accatctcga ccagtgaacg ccccagcgca gagagttttt
    21601 catcgctgag tatttcgtat aaatgaaaac gcgtacgggc gctgtcgccg gggacataaa
    21661 gtagctgttt accctgatcg ttaaccagtt cacgagcttc aatcatcatc accactaacg
    21721 ggtaataatc gacccgatgc tgacggcacc attgcaatat taaagcatcc gcctgggtgg
    21781 agggatactc gcgccagtag tcacaaagtt ctgccagcaa gctttcgcct tctccacaat
    21841 agacgcgttg cgcgtattca taataacgcg catcttcatt atcaggttca gcttcccgcc
    21901 acgaccgggc gtaatccaga agttcgggga tatccaggcg acctgcgcta taccagcgga
    21961 gtaaatttct gtgcagtttt gcatcatcgg ggatgatcac tgcgcgatgt tgcgctaaat
    22021 acaatgtcag ccattctgga tgcgatgacc aaatccgagc gaacatttgg taaaaatcga
    22081 tggtgtcatt ttgcacggca acgggcagat gcagaatggt ctggaagtta aaaacattcc
    22141 ccttcgcaat ggcatataaa aaggattcca gttcttcttc gtctatttcc ccgacgtttt
    22201 cctcctgcaa ccaccgcaat ctttctgcta ataacaccac gcactcgaag gataaatggg
    22261 cagtgttcat ggcgattgtg cacagcgacc agcgtaattc atcaatctct tccatcgagc
    22321 aataatttaa ttgctgaatg aatcgctgcc aggcggaggg cagaaaacgt tcgctatcag
    22381 aggcaagtaa cgcgcgaaag gcgagcagaa tttcatgttc tgctacctca tattcttctg
    22441 gttgccaaac agatttagca ggcgactgcg caatccggag cgcctcctca tacgcctgac
    22501 gaagttgttt aaaaccctgc ggatcggttt ctggatgaaa ggacagtaac agcgccagat
    22561 aagcgcggcg gataatatcg acgtcagtcg tttcctctat ttcgaggatc ttccagcaat
    22621 ttttcatagg tcgctcatat ccttaagcgt ttccgggcat gtggggtagt cagccagtgg
    22681 ttgccaggac aaggcagggt aatacgcatc atcactggca aataattgat aaaacatatc
    22741 attgccggaa tcgtcgtatt ctttacgagc taagaattcg cgtaataaag gcagatgatc
    22801 atcttcggga gactgccaat attgccgacc aaaggtataa ccggcaaagt attgccgcca
    22861 gttactataa taacgtagag cccgtagatg aatacgcgac tggatccata gactttcttc
    22921 ttcactcaac cagttattaa gtacgcccat tcgacttaaa aaacccatcc ggacataatc
    22981 ccatgcttta attcctccct caccacagca ttcggctgtg ctggcgacaa attgcgcata
    23041 agcctgacct tgctcattta gttcagcaag atagtcacgc cattcacatg gcgaataacg
    23101 aaaccagcgg tgataaaacc ctgccaaccg agcggcatga ccgttatcga tcattcggtg
    23161 aattgtgcgc agcaggtcat cacggtcatc aattccccat gaggaagaaa ggtcaaaaaa
    23221 gcatgtacct aaatacattt tgggggatga atgcgaaggt aaacagcatt cattgcgtat
    23281 ttccatcgga gtggaaagtg caaacaatat tttttgcgac cccatgtcca tataatttat
    23341 ccttaaacat tcatttaatg aatgtaaact acatttcgag actcagttat ttatactttt
    23401 ctgacgcaac ataatacgca taaaataagg cttacgcaaa aatggaacac ataaaaatat
    23461 aatatgtttt atctacaacc ataaggaaat gataatggtt tttacgtcaa gttgctgcga
    23521 taatttatca atagatgaga ttatcgaacg tgctgaaaaa ggcgattgcg aggctcaata
    23581 tattgttggg ttttattata atcgcgatag cgcaattgac tctccggacg acgaaaaggc
    23641 cttttactgg ctgaagctgg ccgctgagca aggtcattgt gaagcacagt attccttagg
    23701 gcaaaagtat accgaggata aaagccgtca taaagataat gagcaagcca tcttctggct
    23761 gaaaaaagct gccctacaag gccatacttt cgcttccaac gcccttggct ggacactgga
    23821 tcgtggagaa gcccccaatt ataaagaagc ggttgtctgg tatcagatag ccgcggagag
    23881 cggaatgtct tatgcgcaaa ataatcttgg gtggatgtac agaaatggca acggagtcgc
    23941 aaaagactat gcgctggcat ttttctggta caaacaagct gcattacaag gccatagtga
    24001 cgcgcaaaac aatctggccg atctttatga agacggaaaa ggcgttgctc aaaacaagac
    24061 actcgccgca ttctggtatt tgaaaagcgc acagcagggt aatcggcacg cccagtttca
    24121 aattgcatgg gattataacg ctggcgaagg ggtggaccag gactataagc aagcgatgta
    24181 ctggtatctg aaggctgccg ctcaggaaag cgtcggcgct tacgtcaaca tcggttatat
    24241 gtataaacac ggacaaggcg ttgagaaaga ttatcaggct gcctttgaat ggtttacgaa
    24301 agccgctgaa tgcaatgacg ccactgcctg gtataacctg gccattatgt atcactacgg
    24361 agaaggaaga cctgtcgatc tccgacaggc tctcgacctg tatcgtaaag ttcagtcatc
    24421 cggaaccaga gatgtcagtc aggaaattcg tgagactgaa gatttactgt agagataaaa
    24481 acaagggccg gggaatttca ttcggcccat attctcttta ttgctgtgcg aaatcagact
    24541 attacggctc aaacgggcgt ctctggaact ggtcatgacc acatttcggg catagcgtca
    24601 gcacttccgg tgtgtagatc gggagatgga agtgacattt ctcgcagacc agatttccca
    24661 gcccgaccac ttctccgctg tgataaaccc catgatgatt gaggtcctgg aaaacttcgc
    24721 gccattcaag ctgcgtttta tcggtgatat ctgccagctc ctgccataag ctttctttaa
    24781 tcacccgcat aaagacgctg tcagattctt ctttcaggct ctcttcatag ctcatggcga
    24841 actcttccag gtcacgtctt acagctcgcg tcagctcatc gacctcggtt cgcgttaact
    24901 cccctgtttt tattacgcgc tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc
    24961 cattgcgcag gcgttcgctc agtgacgcga ccagttcacg gtaatattga gcaaccttgt
    25021 tcatcgtttc atctcccggg tcgttaactg actataataa tagacgttct ttgttccttg
    25081 ctttgctaat acgcccacaa actctgtaaa ttaatgtatg aataatccgg gcatcatttc
    25141 aacgtcgagt gcgcgaaagg ctgttttgac gcggacgttt gggctatgct atgcggatct
    25201 gaaaaaccac ctcaacgcta catttgtagc cgtattgaaa acaggaccac tggctgccat
    25261 gcaagagcaa taccgcccgg aagagataga atccaaagta cagcttcatt gggatgagaa
    25321 gcgcacattt gaagtaaccg aagacgagag caaagagaag tattactgcc tgtctatgct
    25381 tccctatcct tctggtcgac tacacatggg ccacgtacgt aactacacca tcggtgacgt
    25441 gatcgcccgc taccagcgta tgctgggcaa aaacgtcctg cagccgatcg gctgggacgc
    25501 gtttggtctg cctgcggaag gcgcggcggt gaaaaacaac accgctccgg caccgtggac
    25561 gtacgacaac atcgcgtata tgaaaaacca gctcaaaatg ctgggctttg gttatgactg
    25621 gagccgcgag ctggcaacct gtacgccgga atactaccgt tgggaacaga aattcttcac
    25681 cgagctgtat aaaaaaggcc tggtatataa gaagacttct gcggtcaact ggtgcccgaa
    25741 cgaccagacc gtactggcga acgaacaagt tatcgacggc tgctgctggc gctgcgatac
    25801 caaagttgaa cgtaaagaga tcccgcagtg gtttatcaaa atcactgctt acgctgacga
    25861 gctgctcaac gatctggata aactggatca ctggccagac accgttaaaa ccatgcagcg
    25921 taactggatc ggtcgttccg aaggcgtgga gatcaccttc aacgttaacg actatgacaa
    25981 catgctgacc gtttacacta cccgcccgga cacctttatg ggttgtacct acctggcggt
    26041 agctgcgggt catccgctgg cgcagaaagc ggcggaaaat aatcctgaac tggcggcctt
    26101 tattgacgaa tgccgtaaca ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa
    26161 aggcgtcgat actggcttta aagctgttca cccattaacg ggcgaagaaa ttcccgtttg
    26221 ggcagcaaac ttcgtattga tggagtacgg cacgggcgca gttatggcgg ttccgggtca
    26281 cgaccagcgc gactacgagt ttgcctctaa atacggcctg aacatcaaac cagttatcct
    26341 ggctgctgac ggctctgagc cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct
    26401 gttcaactct ggcgaattca acggtcttga ccatgaagcg gccttcaacg ccatcgccga
    26461 taaactgact gcgatgggcg ttggcgagcg taaagtgaac taccgcctgc gcgactgggg
    26521 tgtttctcgt cagcgttact ggggcgcgcc gattccgatg gtgacgctgg aagacggtac
    26581 cgtaatgccg accccggacg accagctgcc ggtgatcctg ccggaagatg tggtgatgga
    26641 cggcattacc agcccgatta aagcagatcc ggagtgggca aaaactaccg ttaacggtat
    26701 gccagcgctg cgtgaaaccg acactttcga cacctttatg gagtcctcct ggtactatgc
    26761 gcgctacact tgcccagagt acaaagaagg tatgctggat tccgaagcgg ctaactactg
    26821 gctgccggtg gatatctaca ttggtggtat cgaacacgcc attatgcacc tgctctactt
    26881 ccgcttcttc cacaaactga tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa
    26941 acagttgctg tgtcagggta tggtgctggc agatgccttc tactatgttg gcgaaaacgg
    27001 cgaacgtaac tgggtttccc cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat
    27061 cgtgaaagcg aaagatgcgg caggccatga actggtttat accggcatga gcaaaatgtc
    27121 caagtcgaag aacaacggta tcgacccgca ggtgatggtt gaacgttacg gcgcggacac
    27181 cgttcgtctg tttatgatgt ttgcttctcc ggctgatatg actctcgaat ggcaggaatc
    27241 cggtgtggaa ggggctaacc gcttcctgaa acgtgtctgg aaactggttt acgagcacac
    27301 agcaaaaggt gatgttgcgg cactgaacgt tgatgcgctg actgaagatc agaaagcgct
    27361 gcgtcgcgat gtgcataaaa cgatcgctaa agtgaccgat gatatcggcc gtcgtcagac
    27421 cttcaacacc gcaattgcgg cgattatgga gctgatgaac aaactggcga aagcaccaac
    27481 cgatggcgag caggatcgcg ctctgatgca ggaagcgctg ctggccgttg tccgtatgct
    27541 taacccgttc accccgcaca tctgcttcac gctgtggcag gaactgaaag gcgaaggcga
    27601 tatcgacaac gcgccgtggc cggttgctga cgaaaaagcg atggtggaag actccacgct
    27661 ggtcgtggtg caggttaacg gtaaagtccg tgccaaaatc accgttctgg tggacgcaac
    27721 ggaagaacag gttcgcgaac gtgctggcca ggaacatctg gtagcaaaat atcttgatgg
    27781 cgttaccgtg cgtaaagtga tttacgtacc aggtaaactc ctcaatctgg tcgttggcta
    27841 agcgcgggag gaagcgtgcg atatctggca acattgttgt tatctctggc ggtgttaatc
    27901 accgccgggt gtggctggca tctgcgtgat accacgcagg ttccttccac tatgaaggtc
    27961 atgatcctgg actcaggcga tccgaacggg ccattaagcc gtgcagtgcg taaccagtta
    28021 cgtctgaatg gtgtcgagtt gcttgataaa gaaaccacgc gtaaggacgt tccatccctg
    28081 cgtttgggta aagtgagcat cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca
    28141 gcagagtatc agatgatcat gacggtgaat gcgaccgtgt tgatccccgg ccgtgatatc
    28201 tacccgatta gcgccaaagt cttccgttcg ttctttgata acccgcaaat ggcgttagcg
    28261 aaagataacg aacaagacat gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg
    28321 attcgtaagc tgccaagcat ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg
    28381 accacaacgg atactccggc aacgcctgca cgcgtctcca ccacgctggg taactgatga
    28441 ttcggttgta cccggaacaa ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc
    28501 ttttacttgg taacgatcct ctgttattgc aggaaagcca ggacgctgtt cgtcaggtag
    28561 ctgcggcaca aggattcgaa gaacaccaca ctttttccat tgatcccaac actgactgga
    28621 atgcgatctt ttcgttatgc caggctatga gtctgtttgc cagtcgacaa acgctattgc
    28681 tgttgttacc agaaaacgga ccgaatgcgg cgatcaatga acaacttctc acactcaccg
    28741 gacttctgca tgacgacctg ctgttgatcg tccgcggtaa taaattaagc aaagcgcaag
    28801 aaaatgccgc ctggtttact gctcttgcga atcgcagcgt gcaggtgacc tgtcagacac
    28861 cggagcaggc tcagcttccc cgctgggtta ctgcgcgcgc aaaacagctc aacttagaac
    28921 tggatgacgc ggcaaatcag gtgctctgct actgttatga aggtaacctg ctggcgctgg
    28981 ctcaggcact ggagcgttta tcgctgctct ggccagacgg caaattgaca ttaccgcgcg
    29041 ttgaacaggc ggtgaatgat gccgcgcatt tcaccccttt tcattgggtt gatgctttgt
    29101 tgatgggaaa aagtaagcgc gcattgcata ttcttcagca actgcgtctg gaaggcagcg
    29161 aaccggttat tttgttgcgc acattacaac gtgaactgtt gttactggtt aacctgaaac
    29221 gccagtctgc ccatacgcca ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc
    29281 gccggggcat gatgggcgag gcgttaaatc gcttaagtca gacgcagtta cgtcaggccg
    29341 tgcaactcct gacacgaacg gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg
    29401 cagagctgaa agggttatct cttctgttgt gccataaacc cctggcggac gtatttatcg
    29461 acggttgata tgaaatcttt acaggctctg tttggcggca cctttgatcc ggtgcactat
    29521 ggtcatctaa aacccgtgga aacgctggcg aatttgattg gtctgacgcg ggtcacaatc
    29581 atccctaata atgttcctcc gcatcgtccc cagccggaag cgaacagcgt gcagcgtaaa
    29641 cacatgcttg aactggcgat tgccgacaag ccattattta ctcttgatga acgcgagcta
    29701 aagcgcaatg ccccctctta cactgcgcaa acactgaaag agtggcggca ggaacaagga
    29761 ccggacgtgc cgctggcgtt tattattggt caggattcac tgctgacctt tccgacctgg
    29821 tacgaatacg aaacgatact cgacaatgca catttgatcg tctgtcggcg tccaggttac
    29881 ccacttgaaa tggcgcaacc gcaataccag caatggctgg aagatcattt gacacataac
    29941 ccggaagatc ttcaccttca gcctgccggt aaaatttatc tggctgaaac gccgtggttt
    30001 aacatctcgg cgaccatcat ccgcgaacgt ttgcaaaacg gtgaatcgtg tgaggattta
    30061 ttgccggaac cggtactgac ttacattaac caacaaggct tgtatcgctg atacctgtcg
    30121 ttacggaggg catgatgcga ctgtggttaa ttcgtcatgg tgaaacgcaa gcgaatatcg
    30181 atggtcttta cagcggtcat gcgcccaccc ccctgaccgc gcgcggtatt gagcaagcgc
    30241 aaaatctgca tacgctgcta catggtgttt cctttgatct ggttttatgc agtgaactgg
    30301 aacgggcaca gcataccgca cgactggttc tcagtgaccg ccagctcccc gtgcaaatca
    30361 tccctgaact caacgaaatg ttttttggcg actgggagat gcgacatcat cgcgacctca
    30421 tgcaagaaga tgccgaaaac tatagcgcgt ggtgcaatga ctggcagcat gcaatcccca
    30481 cgaacggtga aggatttcag gcattttcgc aacgtgtgga acgctttatc gcaaggctta
    30541 gtgaatttca gcactatcag aatattttag tcgtcagcca tcagggtgta ctgagtctgt
    30601 taatcgcccg tttaattggc atgcctgccg aggctatgtg gcattttcgc gttgactagg
    30661 ggtgttggag cgccattgat atcaaccaaa aattcgcgac gctacgcgtc ctcaatagcc
    30721 gtgccatcgg ggtcgagaat gcatgacttt tctgtttttt tacgggtaag ccgcaacgac
    30781 cattgacaga cccgggcagg ctgatattct ccgcagccag acttttttcg ccagacacga
    30841 ctttgtagaa attgttttac aaaaatggcg atgcaatctg cggcgcgggg tgggatgata
    30901 gcccactttc gaaagccgat tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat
    30961 actgacagac catttttatc tatttgattc acccaggggg aaaacttgca gggtaaagca
    31021 ctccaggatt ttgttatcga caaaattgat gacctcaaag gtcaggacat catcgcctta
    31081 gacgttcaag gcaaatccag catcaccgac tgcatgatca tctgtacggg tacgtccagc
    31141 cgtcatgtta tgtccattgc tgaccacgtt gtgcaggagt ctcgcgcagc gggcctgtta
    31201 ccgctcggcg tagaaggtga aaacagcgcc gactggattg tcgtggattt gggcgatgtg
    31261 attgtccatg tcatgcagga agagagccgt cgcctgtatg aactggaaaa actctggagt
    31321 taatgcgtga agctgcaact tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc
    31381 ggttttaccg agtacctgcg tcgttttccg aaagatatgc ccttcgagct gattgaaatt
    31441 ccggccggaa aacgcggcaa gaatgcggac atcaagcgca tactcgacaa agagggtgag
    31501 cagatgttgg cggccgcagg caaaaaccgc attgtcaccc tcgatattcc aggcaagccc
    31561 tgggatacgc cgcagttagc cgctgagctg gaacgctgga agctggatgg tcgcgacgtc
    31621 agtctactga ttggcgggcc tgaagggttg tcgcctgcct gtaaagcggc ggcggagcag
    31681 agctggtcgc tgtcggcgct taccctcccc catccgctgg ttcgcgtgct ggtcgcagag
    31741 agtctgtacc gggcgtggag catcaccacc aaccatcctt atcaccgtga gtgataaggg
    31801 agctttgagt agaaaacgca gcggatgaaa ctacagaact cttttcgcga ctatacggca
    31861 gagtccgcgc tgtttgtgcg ccgggcgctg gtcgcctttt tggggatttt gctgctgacc
    31921 ggcgtgctta tcgccaacct gtataatctg caaattgttc gctttaccga ctaccagact
    31981 cgctctaatg aaaaccgcat taagctggtg cctatcgcgc ccagccgcgg cattatctac
    32041 gaccgtaatg gtatccctct ggccctcaac cgcactatct accagataga aatgatgcca
    32101 gagaaagtcg ataacgtgca gcaaacgctg gacgctttgc gcagcgtggt tgatctgacc
    32161 gatgacgata ttgctgcatt ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt
    32221 ccggtgaaaa ctaacctgac cgaagtacaa gtagctcgct ttgccgtcaa tcagtaccgt
    32281 tttccgggtg tcgaagttaa aggctataaa cgtcgttact atccttacgg ttcggcgttg
    32341 acccacgtca tcggctatgt gtcgaaaatc aacgataaag acgtcgaacg cctgaataat
    32401 gacggcaaac tggccaacta tgcggcaacg catgatatcg gtaagctggg cattgagcgt
    32461 tactatgaag acgtgctgca cggtcagacc ggttatgaag aggttgaagt taacaaccgt
    32521 gggcgtgtta tccgccagtt aaaagaagta ccaccgcaag ccggacacga tatttacctg
    32581 acgctggatc tcaaactcca gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct
    32641 gtggttgtca ccgatccgcg tacaggtggg gtgctggcgc tggtttccac gcctagttat
    32701 gacccaaacc tgtttgttga cggtatctcc agcaaagatt attccgcctt gttgaacgcc
    32761 ccgaatacac cgctggtgaa ccgcgccaca cagggggttt atcctcccgc gtctacagtt
    32821 aaaccctatg tggcggtttc ggcattgagc gccggggtga tcacgcgcaa tacgacgctg
    32881 tttgacccag gctggtggca actgccaggt tcggaaaaac gttatcgtga ctggaaaaaa
    32941 tggggccacg ggcgtctgaa tgtcacaaga tcgctggaag aatctgcgga taccttcttc
    33001 tatcaggtgg cctacgatat ggggatcgat cgcctctccg aatggatggg taaattcggt
    33061 tatggtcatt acaccggtat cgacctggcg gaagaacgtt ccggcaacat gcctacccgc
    33121 gaatggaaac agaaacgctt taaaaaaccg tggtatcagg gtgacaccat tccggttggt
    33181 atcggtcagg gttactggac agcgacccca atccagatga gtaaggcact gatgatcctg
    33241 attaatgacg gtatcgtgaa ggttcctcat ttgctgatga gcaccgccga agacggcaaa
    33301 caggtgccat gggtacagcc gcatgaaccg cccgtcggcg atattcattc cggttactgg
    33361 gagctggcga aagacggtat gtacggtgtt gctaaccgcc ctaacggtac ggcgcataaa
    33421 tactttgcca gcgcaccgta caaaattgcg gcgaaatccg gtaccgctca ggtcttcggt
    33481 ctgaaagcga acgaaaccta taatgcgcac aaaattgccg agcgtttacg tgaccacaaa
    33541 ctgatgaccg cctttgcgcc atacaacaat ccgcaagtgg ctgtcgccat gattctggag
    33601 aacggtggtg cgggtccggc ggttggtaca ctgatgcgcc agatcctcga ccacattatg
    33661 ctgggtgata acaacaccga tctgcctgcg gaaaatccag cggttgccgc agcggaggac
    33721 cattaatcat gacggataat ccgaataaaa aaacattctg ggataaagtc catctcgatc
    33781 ccacaatgct gctgatctta ctggcattgc tggtttacag cgccctggtt atctggagcg
    33841 ccagcggtca ggatattggc atgatggagc gtaaaatcgg ccaaatcgcg atgggtctag
    33901 tcatcatggt ggtgatggcg caaattcctc cacgcgttta tgaaggctgg gccccctatc
    33961 tctatatcat ctgtattatt ttgctggtgg cggtagatgc tttcggtgcc atctctaaag
    34021 gtgctcaacg ctggctggac ctcggtattg ttcgttttca gccgtcggaa attgccaaaa
    34081 tagccgtacc actgatggtt gcgcgcttta tcaaccgcga cgtttgcccg ccatcgttga
    34141 agaacactgg catcgcgctg gtgctgatat ttatgcccac gctgctggtg gctgcacagc
    34201 ctgacctggg aacatcaatc ctcgttgcgc tttccggtct gtttgtactg ttcctctctg
    34261 gccttagctg gcgtctgatt ggcgtcgcag tagtgctggt ggcggcgttc attccgattc
    34321 tgtggttctt cctgatgcat gattaccagc gccagcgcgt aatgatgctc ctagacccgg
    34381 aatcagaccc actcggcgcg ggctatcaca ttattcagtc taaaattgct attggctccg
    34441 gcggattacg cggcaaaggc tggctgcacg gcactcagtc acagcttgaa tttctccccg
    34501 aacgccatac cgactttatc ttcgcggtac tggcggaaga gctgggatta gtgggcattc
    34561 tgattctgct cgctctctac attctgctga tcatgcgcgg gctgtggata gccgccagag
    34621 cgcaaaccac ctttggtcgc gtcatggctg gtggcttaat gctgatatta ttcgtttatg
    34681 tcttcgtaaa tattggtatg gtaagcggta ttctgccggt tgtaggggtt ccgctcccac
    34741 tggtcagtta tggaggatcg gcgctaattg tgctgatggc tgggttcggg attgtaatgt
    34801 caatccacac ccacaggaaa atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag
    34861 cagtggctcg ggatctgcat cgcggcagga atgctcgcgg catgtacaag cgatgatggt
    34921 cagcaacaga cggtaagtgt accgcagcct gcggtatgta acggccccat agttgaaatt
    34981 agcggggcgg acccgcgttt cgaaccactg aacgcgacgg caaatcagga ttaccagcgc
    35041 gacggtaaaa gctacaaaat cgtgcaggat ccgtctcgat ttagccaggc gggactggcg
    35101 gcaatctatg atgccgaacc cggcagtaac ctgacggcct ctggcgaagc tttcgatccg
    35161 acacagctga cggcggccca tccaacgctt ccgatcccca gctacgccag aatcactaac
    35221 ctggctaacg ggcgaatgat cgtggtgcgc attaatgatc gcggccctta cggcaacgac
    35281 agcgttattt cactttctcg cgcggcagct gaccgtctta acacgtcaaa caacaccaaa
    35341 gttcgtatcg atccgattat tgtcgcccag gatggttcgc tttctggtcc tggtatggct
    35401 tgtaccacag tcgccaaaca gacttacgcc ctgccagcac ctcccgattt aagcggtggc
    35461 gcgggaacaa gttcagtgtc tggcccgcag ggtgacattc ttccggtcag taattcgacg
    35521 ctaaaaagcg aagatccgac cggcgcgccg gtaaccagta gcggtttcct cggcgctcca
    35581 acgaccttag cgcctggtgt actggaaggc agcgaaccga cgcctgcgcc acagcccgtt
    35641 gttacagctc cgtcgacaac gcctgcaacc tcgcctgcaa tggtgacacc gcaagccgcc
    35701 tcgcaaagcg ccagcggcaa ctttatggtg caagtcgggg ccgtaagcga tcaggctcgt
    35761 gcgcaacagt accaacagca actgggacag aagttcggcg tccccggtcg cgtaactcaa
    35821 aatggcgcgg tctggcggat ccagcttggc ccattcgcca gcaaagccga agccagtacc
    35881 ttgcagcaac gtttgcaaac cgaagcccaa ttacagtcat ttattactac cgcgcagtag
    35941 cgtaaagcag gcatctgatg tgtcaatttc tgtaaatgac gttaacaaag tcacgcggaa
    36001 agtcagatgc ctgccggtag tgcatttgct atagtagggc acttttttaa ttccatcacg
    36061 gatgtcgtag ttcagaccat gaataccatt ttttccgctc gtatcatgaa gcgcctggcg
    36121 ctcaccacgg ctctttgcac agcctttatc tctgctgcac atgccgatga cctgaatatc
    36181 aaaactatga tcccgggtgt accgcagatc gatgcggagt cctacatcct gattgactat
    36241 aactccggca aagtgctcgc cgaacagaac gcagatgtcc gccgcgatcc tgccagcctg
    36301 accaaaatga tgaccagtta cgttatcggc caggcaatga aagccggtaa atttaaagaa
    36361 actgatttag tcactatcgg caacgacgca tgggccaccg gtaacccggt gtttaaaggt
    36421 tcttcgctga tgttcctcaa accgggcatg caggttccgg tttctcagct gatccgcggt
    36481 attaacctgc aatcgggtaa cgatgcttgt gtcgccatgg ccgattttgc cgctggtagc
    36541 caggacgctt ttgttggctt gatgaacagc tacgttaacg cactgggcct gaaaaatacc
    36601 cacttccaga cggtacatgg tctggatgct gatggtcagt acagctccgc gcgagatatg
    36661 gcgctgatcg gccaggcatt gatccgtgac gtaccgaatg aatactcgat ctataaagaa
    36721 aaagaattta cgtttaacgg tattcgccag ctgaaccgta acggcctgtt atgggataac
    36781 agcctgaatg tcgacggcat caaaaccgga cacactgaca aagcaggtta caaccttgtt
    36841 gcttctgcga ctgaaggcca gatgcgcttg atttctgcgg tgatgggcgg acgtactttt
    36901 aaaggccgtg aagccgaaag taaaaaactg ctaacctggg gcttccgttt cttcgaaacc
    36961 gttaacccac tgaaagtagg taaagagttc gcctctgaac cggtttggtt tggtgattct
    37021 gatcgcgctt cgttaggggt tgataaagac gtgtacctga ccattccacg tggtcgcatg
    37081 aaagatctga aagccagcta tgtgctgaac agcagtgaat tgcatgcgcc gctgcaaaag
    37141 aatcaggtcg tcggaactat caacttccag cttgatggca aaacgatcga gcaacgcccg
    37201 ctggttgtgt tgcaagaaat cccggaaggt aacttcttcg gcaaaatcat tgattacatt
    37261 aaattaatgt tccatcactg gtttggttaa aaattgaaca cttgaaagtg taatttccgt
    37321 ccccatatac taagcatcag taaaaaactc ccgccttctg gcgggagttg ctatttaatt
    37381 acgttacgcc ggagctgaca tgaaaaccaa acttaacgaa ctgcttgaat tccctactcc
    37441 ttttacttac aaagttatgg ggcaggcgtt acctgagctg gttgatcagg tggttgaagt
    37501 ggtacagcgc catgcgccag gtgactacac cccaacggta aaaccaagca gcaaaggcaa
    37561 ctaccactcg gtatctatca ctatcaacgc cactcatatc gagcaggttg aaacactgta
    37621 tgaagaacta ggcaaaatcg atattgtccg catggttctg taactcgctt ctccgttacc
    37681 cggcccctgg tcgggtaact cccctctcct cgctgtgata tacttttccc ccacttttac
    37741 tcattctcca cggagatgca gttttgtatc aggataaaat tcttgtccgc cagctcggtc
    37801 ttcagcctta cgagccaatc tcccaggcta tgcatgaatt caccgatacc cgcgatgata
    37861 gtacccttga tgaaatctgg ctggtcgagc actatccggt attcacccaa ggtcaggcag
    37921 gaaaagcgga gcacatttta atgccgggtg atattccggt gatccagagc gatcgcggtg
    37981 ggcaggtgac ttatcacggg ccggggcaac aggtgatgta tgtgttgctt aacctgaaac
    38041 gccgtaaact cggtgtgcgt gaactggtga ccttgcttga gcaaacagtg gtgaataccc
    38101 tggctgaact gggtatagaa gcgcatcctc gggctgacgc gcctggtgtc tatgtcgggg
    38161 aaaagaaaat ttgctcactg ggtttacgta ttcgacgcgg ttgttcattc cacggtctgg
    38221 cattaaacgt caatatggat ctttcaccat ttttacgtat taatccttgt gggtatgccg
    38281 gaatggaaat ggctaaaata tcacaatgga aacccgaagc gacgactaat aatattgcac
    38341 cacgtttact ggaaaatatt ttagcgctac taaacaatcc tgacttcgaa tatattaccg
    38401 cttaattgca tacctcaatg gcccaattta cactgggtca ttactcataa tacctgctaa
    38461 tgctttacat ctccttcgcg tttgtatact ttctttatca cttgcagaaa tgcggtgcta
    38521 cgccttgctc cgtaactcac cccattgatt aacctattga aatagttgat tatggtgagc
    38581 aaaagcgttc cgttccaatt agacataaga ttattgtaat ttttttaaat attatttttc
    38641 catgaataaa attggagtaa gtgcgtggat agtaataatc aaattgaacc ctgtttaagc
    38701 agaaagtcgt cagaaggtaa accacaaata tttacaacct tacgaaatat tgatcttaac
    38761 cttctgacta tttttgaagc tgtatatgta cataaaggga tcgttaatgc agcgaaagtg
    38821 cttaatctga ccccctcggc aatcagtcag tctattcaga aactgcgcgt tatattccct
    38881 gacccattgt ttattcgcaa aggccagggt gtcactccta ccgcatttgc gatgcatcta
    38941 catgagtata tcagtcaggg ccttgagtcc attcttggcg cgctggatat cgaaggaagc
    39001 tatgataagc aacgaacgat aactattgcg accactccct cggtcggagc cctggtcctt
    39061 cctgtcatct atcgggcgat taaaactcac tatccgcagc ttttactgcg caacccgccc
    39121 atcagcgacg cggaaaacca actcagtcag tttcaaaccg atctcatcat cgataacatg
    39181 ttttgcacca atcgtacggt gcaacatcat gttctgttca ccgacaatat ggtgttaatt
    39241 tgccgcgagg gaaatccact actctcttta gaagatgaca gagagactat cgacaacgct
    39301 gcgcatgtac tcctgttacc ggaagggcaa aatttcagcg gtctgcggca gagagttcaa
    39361 gagatgtttc cggaccggca aatcaatttc accagctaca acattttgac aatcgctgca
    39421 ctggttgcca acagtgacat gttagcgatt attcccagcc gtttttataa cctgtttagt
    39481 cgttgctggc cgctggaaaa actgcctttt ccgtccttaa atgaggagca aatagatttc
    39541 tctatccact acaacaaatt cagcctgcgt gatcccatcc tgcacggggt aatagatgtt
    39601 atccgtaatg cattttgaaa aagtattgtt acagccagat cacagcgcag aacgataaaa
    39661 tggcacaatc caacaacaat tttacatttt cgcgaccgct ttggctgctt tcaggtccgt
    39721 ttcaatgata tactgccagt cgttaattca aaaatagttg ataattacaa caatctattg
    39781 aattgaaacg ctttccttcg taattcgcaa ctggaacacg cacgctatga gtaaacccat
    39841 tgtgatggaa cgcggtgtta aataccgcga tgccgataag atggccctta tcccggttaa
    39901 aaacgtggca acagagcgcg aagccctgct gcgcaagccg gaatggatga aaatcaagct
    39961 tccagcggac tctacacgta tccagggcat caaagccgca atgcgcaaaa atggcctgca
    40021 ttctgtctgc gaggaagcct cctgccctaa cctggcggaa tgcttcaacc acggcacagc
    40081 aacgtttatg atcctcggcg ctatttgtac ccgccgttgt ccgttctgtg atgttgccca
    40141 cggtcgcccg gtagctcctg atgccaatga accagtgaaa ctggcgcaga ccattgccga
    40201 tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt gatgacctgc gcgatggcgg
    40261 tgcccagcac tttgcggatt gcattactgc cattcgggaa aaaagcccgc aaatcaaaat
    40321 tgaaactctg gtgccggatt tccgtggtcg tatggatcgt gctctggata ttctgactgc
    40381 aacgccacca gatgtgttca accataacct ggaaaacgta ccgcgtattt accgtcaggt
    40441 acggcctggt gcagattaca actggtcgct gaagctgctg gaacgcttta aagaagcgca
    40501 tccggaaatc ccgaccaagt ctggtctgat ggtgggactg ggtgaaacca atgaagaaat
    40561 tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg atgttaacgc tggggcaata
    40621 tttgcagcca agccgccatc acctgccggt tcaacgttac gttagcccgg atgagttcga
    40681 cgaaatgaaa gccgaagcgc tggcgatggg ctttacccat gctgcatgcg gtccgtttgt
    40741 ccgctcttct taccacgccg atttgcaggc gaaagggatg gaagttaagt aatcctgtaa
    40801 taattactta catctgtctg ataaaaaacc cgctttttg
//
LOCUS       DAJTYZ010000019        40665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000019 DAJTYZ010000000
VERSION     DAJTYZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.019"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            209..1306
                     /gene="mltA"
                     /locus_tag="OO596_001015"
     CDS             209..1306
                     /gene="mltA"
                     /locus_tag="OO596_001015"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6716933.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1383..2189
                     /gene="tcdA"
                     /locus_tag="OO596_001016"
     CDS             1383..2189
                     /gene="tcdA"
                     /locus_tag="OO596_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6716934.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2240..2683)
                     /gene="csdE"
                     /locus_tag="OO596_001017"
     CDS             complement(2240..2683)
                     /gene="csdE"
                     /locus_tag="OO596_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6716935.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2683..3888)
                     /gene="csdA"
                     /locus_tag="OO596_001018"
     CDS             complement(2683..3888)
                     /gene="csdA"
                     /locus_tag="OO596_001018"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6716936.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4080..4307
                     /locus_tag="OO596_001019"
     CDS             4080..4307
                     /locus_tag="OO596_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6716937.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4658..5575
                     /gene="gcvA"
                     /locus_tag="OO596_001020"
     CDS             4658..5575
                     /gene="gcvA"
                     /locus_tag="OO596_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6716938.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5594..5989
                     /locus_tag="OO596_001021"
     CDS             5594..5989
                     /locus_tag="OO596_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6716939.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5982..7082
                     /gene="rlmM"
                     /locus_tag="OO596_001022"
     CDS             5982..7082
                     /gene="rlmM"
                     /locus_tag="OO596_001022"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6716940.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7126..7857)
                     /gene="fucR"
                     /locus_tag="OO596_001023"
     CDS             complement(7126..7857)
                     /gene="fucR"
                     /locus_tag="OO596_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6716941.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7915..8337)
                     /gene="fucU"
                     /locus_tag="OO596_001024"
     CDS             complement(7915..8337)
                     /gene="fucU"
                     /locus_tag="OO596_001024"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6716942.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8339..9757)
                     /gene="fucK"
                     /locus_tag="OO596_001025"
     CDS             complement(8339..9757)
                     /gene="fucK"
                     /locus_tag="OO596_001025"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR6716943.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9866..11641)
                     /gene="fucI"
                     /locus_tag="OO596_001026"
                     /pseudo
     CDS             complement(9866..11641)
                     /gene="fucI"
                     /locus_tag="OO596_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            complement(11674..12990)
                     /gene="fucP"
                     /locus_tag="OO596_001027"
     CDS             complement(11674..12990)
                     /gene="fucP"
                     /locus_tag="OO596_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6716944.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            13537..14184
                     /gene="fucA"
                     /locus_tag="OO596_001028"
     CDS             13537..14184
                     /gene="fucA"
                     /locus_tag="OO596_001028"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6716945.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14212..15360
                     /gene="fucO"
                     /locus_tag="OO596_001029"
     CDS             14212..15360
                     /gene="fucO"
                     /locus_tag="OO596_001029"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6716946.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15465..16220)
                     /gene="xni"
                     /locus_tag="OO596_001030"
     CDS             complement(15465..16220)
                     /gene="xni"
                     /locus_tag="OO596_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6716947.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16332..17699)
                     /gene="sdaB"
                     /locus_tag="OO596_001031"
     CDS             complement(16332..17699)
                     /gene="sdaB"
                     /locus_tag="OO596_001031"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6716948.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17757..19046)
                     /gene="sdaC"
                     /locus_tag="OO596_001032"
     CDS             complement(17757..19046)
                     /gene="sdaC"
                     /locus_tag="OO596_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6716949.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19602..20966)
                     /gene="ppnN"
                     /locus_tag="OO596_001033"
                     /gene_synonym="ygdH"
     CDS             complement(19602..20966)
                     /gene="ppnN"
                     /locus_tag="OO596_001033"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6716950.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21078..21926)
                     /gene="queF"
                     /locus_tag="OO596_001034"
     CDS             complement(21078..21926)
                     /gene="queF"
                     /locus_tag="OO596_001034"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6716951.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            21994..22539
                     /gene="syd"
                     /locus_tag="OO596_001035"
     CDS             21994..22539
                     /gene="syd"
                     /locus_tag="OO596_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6716952.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23161..23490
                     /locus_tag="OO596_001036"
     CDS             23161..23490
                     /locus_tag="OO596_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6716953.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23490..24272
                     /gene="truC"
                     /locus_tag="OO596_001037"
     CDS             23490..24272
                     /gene="truC"
                     /locus_tag="OO596_001037"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6716954.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24290..24739
                     /locus_tag="OO596_001038"
     CDS             24290..24739
                     /locus_tag="OO596_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6716955.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25174..26526
                     /gene="gudP"
                     /locus_tag="OO596_001039"
     CDS             25174..26526
                     /gene="gudP"
                     /locus_tag="OO596_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6716956.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26528..27868
                     /locus_tag="OO596_001040"
     CDS             26528..27868
                     /locus_tag="OO596_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6716957.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27889..29229
                     /gene="gudD"
                     /locus_tag="OO596_001041"
     CDS             27889..29229
                     /gene="gudD"
                     /locus_tag="OO596_001041"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6716958.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29461..32217)
                     /gene="barA"
                     /locus_tag="OO596_001042"
     CDS             complement(29461..32217)
                     /gene="barA"
                     /locus_tag="OO596_001042"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6716959.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32274..33575
                     /gene="rlmD"
                     /locus_tag="OO596_001043"
     CDS             32274..33575
                     /gene="rlmD"
                     /locus_tag="OO596_001043"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6716960.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33623..35857
                     /locus_tag="OO596_001044"
     CDS             33623..35857
                     /locus_tag="OO596_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6716961.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            35935..36183
                     /gene="mazE"
                     /locus_tag="OO596_001045"
     CDS             35935..36183
                     /gene="mazE"
                     /locus_tag="OO596_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR6716962.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36183..36518
                     /gene="mazF"
                     /locus_tag="OO596_001046"
     CDS             36183..36518
                     /gene="mazF"
                     /locus_tag="OO596_001046"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR6716963.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36589..37380
                     /gene="mazG"
                     /locus_tag="OO596_001047"
     CDS             36589..37380
                     /gene="mazG"
                     /locus_tag="OO596_001047"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6716964.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37608..39245
                     /gene="pyrG"
                     /locus_tag="OO596_001048"
     CDS             37608..39245
                     /gene="pyrG"
                     /locus_tag="OO596_001048"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6716965.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39333..40631
                     /gene="eno"
                     /locus_tag="OO596_001049"
     CDS             39333..40631
                     /gene="eno"
                     /locus_tag="OO596_001049"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6716966.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
BASE COUNT        10072 a         9840 c        10816 g         9937 t
ORIGIN      
        1 tccgtggatg cgcactatac tccgctcgcg ttttcatgca accttttttt taccccgatc
       61 acggatctgg catgattttg aacaaaaaca aatcactaaa gcctattttt tgtgcaaaat
      121 ttgcgtcagg gagaggctgc tcatttcatt aaacgcggcg gtttgttatc ttcgttgcgc
      181 cttatttttt aacctgaaga agagaacaat gaaaggacgt tgggtaaagt accttcttat
      241 gggcacggtt gtggcaatgc ttgccgcctg ctcttccaaa ccaaccgatc gcggacagca
      301 atataaagac gggaaattta cccagccttt ctctctggtg aaccagccag atgccgttgg
      361 cgcgccgatt aacgccggtg attttgccga gcaaattaac catatccgta attcgtcacc
      421 gcgtctgtat ggcaaccaga gtaatgttta taacgcggtg caagagtggc tgcgcgcagg
      481 cggtgatacc cgcaatatgc gccagttcgg cattgatgcc tggcagatgg aaggtgccga
      541 caactatggt aacgtgcagt ttacaggcta ttacacgccg gtaattcagg cgcgccatac
      601 ccgccagggc gagttccagt atcctattta ccgtatgccg ccaaaacgtg gtcgtctgcc
      661 gtctcgtgcg gagatctacg cgggggcatt gagtgataaa tatattctcg cttacagtaa
      721 ctccctgatg gataacttca ttatggatgt gcagggtagt gggtatatcg actttggtga
      781 tggcagtccg cttaactttt tcagctatgc agggaaaaac ggtcatgcct atcgcagcat
      841 tggtaaggtg ctgatcgacc gtggcgaagt gaaaaaagaa gatatgtcga tgcaggcgat
      901 tcgtcactgg ggcgaaacac acagtgaagc cgaggttcgc gagctgctgg aacagaaccc
      961 gtctttcgtc ttctttaaac cgcaatcttt tgcaccggtg aaaggggcaa gtgcggtgcc
     1021 gctggttggt cgcgcgtcag ttgcctctga tcgttccatt attccgccag gtactacctt
     1081 gctggcagaa gtgccgttgc tggataataa cggcaaattt aatggtcagt acgaactgcg
     1141 tctgatggtg gcgctggatg tcggtggtgc aatcaaaggc caacacttcg atatctatca
     1201 agggatcggg ccggaagccg gacaccgcgc aggttggtac aaccactatg gacgtgtctg
     1261 ggtgctgaaa accgccccgg gcgcaggtaa cgtctttagc ggctgatgtg ttattctgta
     1321 taccgttttt acggatacga cagggtgagg gataacctca ccctttttaa ttctgaggtg
     1381 ctatgtctgt ggtaattagt gatgcatggc gtcagcgttt tggtggcaca gcgcgtctgt
     1441 atggtgaaaa agcgttgcaa ctgtttgctg acgcgcatat ttgtgtggtt ggtatcggtg
     1501 gtgtcggttc ctgggcggcg gaagcgctgg cgcgcacggg gattggcgca atcacgctta
     1561 tcgatatgga tgatgtgtgc gtcaccaata ccaatcggca aattcacgct ctgcgcgata
     1621 acgtcgggct ggcaaaagcg gaagtcatgg cggagcgtat tcgccagatt aacccggagt
     1681 gccgtgtaac ggtggtggat gatttcgtga cgccggataa cgtagcgcag tatatgagcg
     1741 tgggttattc gtacgtgatt gatgccattg atagtgtacg gcccaaagcg gcgctgattg
     1801 cttattgtcg gcgcaataaa atcccgctgg tcacgaccgg tggcgcgggt gggcagattg
     1861 acccgacgca gattcaggtt accgatctgg cgaaaacgat tcaggatccg ctggcggcga
     1921 agttgcgcga gcgcctgaaa agcgattttg gcgtagtgaa aaacagtaaa ggtaagctcg
     1981 gcgtcgattg cgtgttttct actgaagcgc tggtgtaccc gcagtcagac ggtactgtgt
     2041 gtgcgatgaa agccacggca gaagggccga agcggatgga ttgtgcatct ggatttggcg
     2101 cggcaacgat ggtgaccgcc acctttgggt ttgttgcggt ttctcatgcg ctgaagaaga
     2161 tgatggcgaa agcggcgcgt cagggttaaa caatcaggtc ggatgagacg cgaacgtctt
     2221 atccgacccg gttctatgct taaacctgct tcgtcgcagc gataatcgcc tcgcttaacg
     2281 catttaaccc ctggctgcgt gaggcgctaa gttgcgcacg caatcccagc tcatcaaaca
     2341 atgccagcgg cgactgtgcc tgcaattcgg cggcggtttt cccctcaacg gcagtcaaca
     2401 gcaccgccag caggccgcgc acaatgcgcc cttcgctatc gccaaagaag tgcattttac
     2461 cgttttcagt cacggtatat cccagccaga cgcggttttc gcatccggca atctctttag
     2521 cctgcgcttt taactcgtct ggcaaagccg gaagctgttt ccccagcatg atcaactggc
     2581 gatatttatc ttcccattgc gacaacggtg cgaaggtatt gcgtaacgtt tctgcggtta
     2641 cggttgtgcc gaacggatgt ccggcgaatt gcgggttcgt cattaatcca ccaataattc
     2701 cagcgcgcgg tcaacggcat tcaccagcgc atccacatca ctctttgtat tatatggcgc
     2761 aaaagaggcg cgcagtgtgc cggttacgcc taattctgcc agtagcggct gagcgcaatg
     2821 ttgcccggcc cgcagggcaa taccgtactc cgccagcagc gtcaccatat cgctgtgatg
     2881 aacgccagca aaatcaaagg ccagcaggct ggaatcctgg cagcggaatg agcggaagcc
     2941 aggacgtttt gccagcgcat cttccgccag cgtcgccagg ctgcgactcc aactttctgc
     3001 ctggttgata tcgtaatctg ccagccattc cagcgccgcg cttaatccta tgacaccagc
     3061 cacatttggc gttcctgctt ccagtttcca cggcgcagat tgagtcgtga agccgtcaaa
     3121 actcacttcg tgaatcattt tgccgccacc cagccagggc gacatcgctt ccagcagttc
     3181 tgatttgcca tacagcacgc cgatacccgt cgggccatac agtttgtgac cagagaaagc
     3241 atagaaatcg atatctagtt gctgaacatc tgcggggaaa tgcaccgccc cctgagcacc
     3301 atcaaccatc accaccatcc cggctgaatg agcaaaggta atcgctcgcg ccagatccgg
     3361 gcaaccgcca gtaacgttcg acatctgacc caacgccaga atccgactac ggggagtaat
     3421 cagttctggc aacaaatcga catccggcag tcgctgcgca ttaagcggca atttcaccac
     3481 tttggctcca gtttgttggg cgaccatcag ccaggggacg aggttagcgt ggtgttctgc
     3541 cacgctgaca ataatctcat cgcccggttg cagacgcgga cgcgcatagc attgtgccac
     3601 catgttgatg gattcagtgg tgccgcgcgt ccagacgata gttttatcat ccggtgcatt
     3661 cagtaattgc gccactttct ctcgtgcagc ttcataacgc gcggtcaggc gttgggcttc
     3721 ggcaaactgg ctgcgatgga cgtttccggc gctcaggctg taaaactgtc gagtggcttc
     3781 aaccacggct tcaggtttaa gcgcggtcgc ggcgctatcg agatagacgc ccgcatcctg
     3841 tagtgcggga aactgggcgc gaaactgcgc gggattaaaa acgttcatgg tactcctcgg
     3901 cttgatgcac cgatcgtgac gcaaaattca ctttgataca aggagagtgc taaccatcag
     3961 aattatctgg atatcctggc gaagccgcgt aagtaacata tgctgaatag tgttaggcgt
     4021 taattttagc ctgttaatca atactattaa gatttattaa tcgctttaag gagaagatga
     4081 tgaaaaagac tgccgcaatt atttctgcct gtatgctgac ttttgccctg agcgcctgtt
     4141 ccggttcgaa ctatgtgatg cacaccaatg acggacgtac catcgtctct gacggcaaac
     4201 cacagactga taacgatacc ggtatgattt cgtataaaga cgctaatggc aacaaacagc
     4261 agatcaaccg tactgacgtg aaagagatgg tcgaactgga tcagtaattc gcgatcgcaa
     4321 ggtaaaaaaa agcaccgcaa ttaggcggtg ctacattaat cactatggac agacagggta
     4381 aatgtacagg aagtgaaaaa aggtagcttt gctaccatgg tctgaatcgc agaccaattg
     4441 caaacacaac aacacaacat cacaaccgta agccaaaagt tcaccagaac acgcattccg
     4501 ataaaacttt tcgttccggc tcaggaagtg ccgccactat aggtatttgc tggtagaagc
     4561 tcaacggaca atttataatg gctcagatta aaaaaactaa taggttacac agtgtgatct
     4621 aattgttaaa ttaatttaac atcaaagttt aaaagccatg tctaaacgat taccaccgct
     4681 aaatgcctta cgagtttttg atgccgcagc acgccattta agtttcactc gcgcagcaga
     4741 agagcttttc gtgacccagg ccgcagtaag tcatcaaatc aagtctcttg aggatttttt
     4801 ggggctaaaa ctgttccgcc gccgtaatcg ttcactcctg ctgaccgagg aagggcaaag
     4861 ctatttcctc gatatcaaag agatattttc gcaattaacc gaagcgacgc gtaaactcca
     4921 ggcccgtagc gccaaggggg cgttgacggt cagtttactc cccagtttcg ccattcattg
     4981 gttggttccg cgactttcca gctttaattc agcttatccg ggaattgacg ttcgaatcca
     5041 ggcggttgat cgtcaggaag ataagctggc ggatgatgtt gatgtggcga tattttatgg
     5101 tcggggcaac tggccggggc tacgggtgga aaaactgtac gccgaatatt tattgccggt
     5161 gtgttcgccg ctactgctga caggcgaaaa acccttgaag accccggaag atctggctaa
     5221 acatacgtta ttacatgatg cgtcacgccg tgactggcag acatataccc gacagttggg
     5281 gttaaatcat atcaacgttc agcaagggcc aatttttagt catagcgcca tggtgctgca
     5341 agcggctatt cacgggcagg gagtggcgct ggcaaataac gtgatggcgc aatctgaaat
     5401 cgaggccgga cgtcttgttt gcccgtttaa tgatgttctg gtcagtaaaa acgcttttta
     5461 tctggtttgt catgacagcc aggcagaact gggtaaaata gccgcctttc gccaatggat
     5521 cctggcgaaa gccgctgctg aacaagaaaa attccgcttt cgttatgaac aataatttac
     5581 gtagggtacg accatgacca gccgttttat gctgattttc gccgccatta gcggcttcat
     5641 ttttgtggct ctgggcgctt ttggcgcgca tgtgttaagt aaaaccatgg gggccgttga
     5701 gatgggctgg atccagaccg gcctcgaata ccaggcgttt catacgctgg cgatcttagg
     5761 tctggcggtg gcaatgcagc gtcgcatcag tatctggttt tactggagta gcgttttcct
     5821 cgcgttaggc acggtgttgt tcagcggcag cctttattgc ctggcgctgt cccatctgcg
     5881 tttgtgggca tttgtcacac cggttggcgg cgtgagcttc ctcgcgggct gggcgttaat
     5941 gttagttggt gctatccgtt taaagcgcaa gggcgtaagt catgaataag gttgtattgc
     6001 tgtgccgtcc gggctttgaa aaagagtgcg ccgcagaaat taccgataaa gccggccagc
     6061 gggaaatttt cggttttgcc cgcgtgaaag agaatgcggg ttatgtcatt tatgaatgtt
     6121 atcaacctga tgatggcgat aagttaatcc gtgagctgcc gttcagttca ttaatttttg
     6181 cccgccagtg gtttgtggtg ggggaactct tgcagcattt gccgccagaa gatcgtatta
     6241 cccccattgt cgacatgttg cagggcgtag tagagaaggg cggtgaactg cgtgttgaag
     6301 ttgccgatac caacgaaagc aaagagttgc tgaaattctg ccgtaaattc accgtgccgc
     6361 tacgcgctgc cttgcgcgat gccggggtgc tggcgaacta tgaaacgccg aagcgtccgg
     6421 ttgtgcatgt attctttatt gcaccaggct gctgctatac cggttactca tacagcaaca
     6481 ataattcgcc gttctatatg ggcattccgc gcctgaaatt tccggcagag gcgccgagtc
     6541 gttccacgct caaactggaa gaggcatttc atgtgtttat tcctgcggat gagtgggatg
     6601 aacgcctggc gaacgggatg tgggcggtgg atttaggcgc ttgccctggc ggctggacct
     6661 accaactggt gaagcgcaac atgtgggttt attccgtcga caacggcccg atggcgcaaa
     6721 gtctgatgga taccgggcag gtgacgtggc tgcgggaaga cggtttcaaa ttccgtccga
     6781 cacgcagcaa tatctcctgg atggtatgcg atatggttga aaaaccggct aaagttgcgg
     6841 cattgatggc gcagtggctg gttaatggct ggtgccgtga aaccattttc aatctcaaac
     6901 tgccgatgaa aaaacgctac gaagaagtat cacacaatct ggcgtatatt caggcacagc
     6961 ttgatgaaca tggcataaat gctcagattc aggcgcggca gttgtatcac gatcgcgaag
     7021 aagtgacggt gcacgtccgc cgcatctggg ctgcggtggg tggtcgtcgc gacgagcgat
     7081 aacaggcaag gccggggcgt cgtcttcggc ctgctaccct ttccgtcagg ctgttaccaa
     7141 agaagttgca acctggcgct catcagaaat aatgtgcgtt acctcatcca gatgcccgat
     7201 gcgggcttcc cctgaacgat taaatttact tttatcaatc aataacaacg attgcgcggc
     7261 acgttttaat agcatcgatt tgtaatcagc gttgatcgca ttggagtccc acagtgcgcc
     7321 gctgctatcg atcccttcac aagaaaaaat aaacagatcg atttccagcg atttaagttg
     7381 ggaaatcagc gagggattga cgtaacagcc atatttgcgc tcaagtgtgc cgccggaact
     7441 gatcagttga atgcgttcgc gtttaccgag ttcatggcaa atcggatggc tattggtgaa
     7501 gacctgaatg ttgatgtcag gcaactggcg tgccagatac cagcaagttg aactggcgtc
     7561 taaggctatc accatccctt cttcaatcca cgcgagcgcc tcgcgcgcga tatctgcttt
     7621 atgcgcataa tggcttttca gcctgatgtg aaagggatcg ccactgtctt gattttgacg
     7681 gtggatatat ttagcgcgtc catgattgcg cagaatttta ccctgcgtct gtaattcatt
     7741 gagatcgcga cgaatggttt ctttactgac ctttagctgt tcagagagag cttccgtggt
     7801 caggctggta tggttcagca gcaggtcgac tatcgcttgc tggcgtgccg ctttcatatt
     7861 tttcacccca tcattcgata accgcccgcg tcatgcgggc gtaccgatat gagattacgg
     7921 tgttacccct ttttttaaaa gaatattccc gtacttcgct cgttcgcctg tgataacgat
     7981 cgcaaaggct ttttgcgccc gttcataaaa cgcaaaacga ttgatgcgga tgatgtcagg
     8041 acacggggct tgtagtgaaa gcgcattacg gtaacgtcgt tctacttcag gatcgagagt
     8101 gtcaccttct accgctgcca tcatcaccag cggcggggca taactgtcca gctcaaataa
     8161 tgggataatc gcttggagca agtcgctcac caacaggcca tcagcgcgga tcacctgcgg
     8221 tcccatcgaa tgggcgggaa agtgagcatc ggaaaaaata atttcatctc catgtcccat
     8281 ctctgccagc actttcaata gttcgggaga aattaacggc gaaattgttt tcagcatttc
     8341 acacttcctc tataaattca ggttcagttt gcgggtagaa ataacggtac tgataatgaa
     8401 tctgtgcacg ggcttcttcc gggctgttaa attcccctac gccataccaa ccgaacagcg
     8461 cagctcctgc gacggtcgtt tcggcatcgt cgagaacttt taccggaata tcaagcatat
     8521 tggctttaat ctgattccac aatgtgttgc gacttcctcc accgactaac aataattcag
     8581 aggctttaaa gtgcccgatt ttttccagca tctgtagatt gcgctgtaat tgcgcagtta
     8641 acccttccag cgccgcgcga tagaaatgcc cccgcgtggt attaagcgtc actccttgcc
     8701 agccagcgtt ctgacacgac aataaatcac actgcatttt cacaccatcc gcgccaggtg
     8761 cgatcagacg agcttcttca atcaacattt gccagggtgt ttcagccgtc cagaacagtt
     8821 ttctcaccca ttccagcacg ccggatgcca gccattgcat acctgggtta tacaaccctg
     8881 cctggctatc cagttcgcag gtagaatcgg cgtactgact taacagcgaa gtatcaacct
     8941 gggcgctacg gaccattaaa atttcccatg taccggaaga gagcacgggt tcattttggt
     9001 cggcaccagc gccaaaaagg gcgaactggg tatcgtgacc tgcggaaatc accggtatgc
     9061 caacgggtaa gccgagcatt gctgcggcgc tgttctgtag cgtaccaatc tgttcacccg
     9121 cttccaccag acgagggaag agtcggcgtg gaataccggt ggcttgtaaa atttgcggac
     9181 tgaaatcgcg ttgctggata tccagcatct ggctggttcc ggccatcgtg atatcagtag
     9241 tgaattcgcc ggttaaacgg tggttaatca gcgacgaaat aaagagccag gcgtgcgcgc
     9301 gttccagcag ttgtggatga ttttctttca accacaccaa cttatataac gtattgaaac
     9361 taaaggctcc gacgccagaa atagcctgca accgctgtgc ggagatcaac tgttcaatat
     9421 tgtccataac cgctgctgtt cgcggacatt tccagctaat gatcggatag agcagattgc
     9481 cttgcttatc taccagagcg ccatccacac caaaggtggt gacggcgata ccgcggatgt
     9541 ggcattcagt cagttcacta ttgatttgcc gacagcaatc agcaaagcgt tgcaaaatgg
     9601 cgtctaaaga ccactggtgc caggtgttgt tttccatcgc gatatcgctg gcattaggcg
     9661 ttgaggcgcg ggcaacaatt tttccctgcc gattaaccgc gatggccctg acattggtcg
     9721 cgccacagtc gagtaccagg ataacttctt gtttcataat cgctcctgca atatagccgg
     9781 ataacattgc ttatccggct aaccactctt gcttgttcag gcccggtagc cggagcgacc
     9841 gggcatcaca tcagggagta atgtattaac gcttgtacaa cggaccgtag ttctggcaag
     9901 cgcggtaatc ctggccttca atatccatgc cgtgcgcagc ccaggcagaa ggacgataca
     9961 ctttggtctc ttcaacgttg tgcatacata ccgggatacg cagcatggag gcgagagtga
    10021 taaagtctgc gccaacgtgg ccgatggtca gaaccccatg gttagcgccc cagttcgcca
    10081 ttaccgagta cacatccgta aacggccctt taccggtgag gcgcggtgca aaccaggtgg
    10141 ttggccaggt tgagttggtg cgtttgttga ggatgtcatg cacatccttc ggcaattcca
    10201 cgctccagcc ttccgcgatt tgcagtaccg atcccaggcc tttgatgatg ttgacacgag
    10261 tcatggtgaa cgggacgccg ccttcggtaa ggaagcggga agagtaaccg ccgccacgga
    10321 agtattcgtg gatcgccggg caccattcgg tagcggcgag gcaagcgtca gcctcttgct
    10381 gagagatttc ccagtgtggc ttcatcgtcg ggttaccttc gctgtcgcgt tgtttacagg
    10441 aaccgtccag cgcagcagaa cctgagttga tcaaatggat gatgccgtgt tctgccagtc
    10501 catcgagttt atgccccgtt acacgctcaa ttgcttctgg tgaccagtag gtacgcacat
    10561 cggcaaatac ctgagcggtg ccggtgagct ggtgacccat tagcattgcc acgccgttaa
    10621 gactgtcgtt ttcggtcgcc acgacaaagg gttcgcgcac gccattccag tcaaatgaac
    10681 tgttgaggat cgcttcggcg gtgtcaccat tgggatattg atcggtccag tgacgttgcc
    10741 cctggaagcc cgcagcgatg gcgttgtagc caagtgattc ttccacgcga ccaatatcgg
    10801 ccagtttgct gttgccttgc atcatgtcgc ggatacacat cgccatcagt aaactttcgc
    10861 gcagaactgc gcggctttgc tcggcattgc gttgatactg tttgttattt tcatcttcgc
    10921 catagcggaa gtttttatca gcccaggtca gtgccatttc cagttcggct tcgtcgtaaa
    10981 tcttctgatc gatacggcga cgcagttcgg tcatatccac cgcctggact ttcattccca
    11041 gccaggattc aaagaagttg tgatcaacaa tagaaccggc gatacccatc gaaacgccgc
    11101 ccagcgacag atagctttta cctttcatgc tggcgacggc caaaccggcg cgggcaaagc
    11161 gcagcagttt ttcttcaaca tcggcaggaa tcgatgtgtc atcggcatcc tgaacgtcat
    11221 gaccgtaaat ggagaatgcc gggatgcctt tctggctgtg agctgccaga gccgctgcca
    11281 ggtaaacagc gccgggacgt tcagtgccgt taaagcccca aatggctttc gggcgggttg
    11341 gatccatgtc gatggtttca ctgccatagc accagcaagg cgttaccgta atggtgaggc
    11401 ctacattcta gctgctgaat ttttcttcgc aagcagcggc ttcagccata cccgcgatac
    11461 aggtatcgga aatgacacac tcgacggcag ctccgcaggc atggcgcagt ttctcggtca
    11521 gcagtgcggc cgtagctttc gccatattca ttgtttgttc ttcaagcgac tcacgaacac
    11581 ccatgcgacg accgtcaata accgggcgga taccaatttt cggtaagctg atttttttca
    11641 ttacagattc ctcactttat tcggaaaata tgttcagtta gttgccgttt gagagcggaa
    11701 acgggcaaag ataaagatga ccgcgaagca gagtgtgggg atcagttcag cagtggggat
    11761 gttgcccgcc gcgtcactga caaaacccat gaccggagtg acaataccgc cgccaataat
    11821 ggtcataacg atgaaggacg aaccgtattt ggtgtcctgg ccgagattct taatgcccag
    11881 cgagaagatt gttgggtact gaatcgacat aaaggcgctg cataaagtca gggctattaa
    11941 gcccacatga ccgccagcga aggctgagat caggcacagt gccatagcga ttaatgcgta
    12001 ggcggccagg actttgtgtg gtgcgaagcg actgatgagc caggtaccgg tgaaacgacc
    12061 aataaagaag cacaccatgg ttccggttaa atagttagcg gcaaagcctg cagtcatacc
    12121 tggaatttct tctacagcgt agcgaatcaa atagctccag caggccgttt gtgcgccaac
    12181 gtagcagaat tgcgctaata ctgcccagcg ccagtggcga atacgcgcta ggcgagaaag
    12241 cgatgcggag aacgatcctt gtttggcgtc actgtgatta tcactctgca atgccgggaa
    12301 tttcgtcagc atgatcagca gggcgaccag taacacgata gccacgatga tcatataagg
    12361 tgtctgtacc gataatacca ggctgtgttt atacgcactc aattgctctg gagacatttt
    12421 atcgagaacg tcttgcgatt gatgtggcac gttagacaaa ataagtcttt gcccaaagac
    12481 aaccgcgata attgcgccaa acgagttaaa tgtttgcgca agatttaagc ggaagtgacc
    12541 actgctttcc ggccctaata ccgtaacaaa agggtttgcg gcagtttcca gacaacctaa
    12601 tccggctgca ataataaata ggccaactaa aaataaggtg tagttcatta tttctgcagc
    12661 gggccagaat aatgcagcgc ccagggcata taaaaataac ccagtaataa ttcctgcttt
    12721 ataactgagt tttttcatca atatcccagc aggaattggg ataatgaaat aaccaaagta
    12781 aaaggccgat tggatcaggc cagcctggaa atttgtcagc gtaaaagcct gctggaattg
    12841 aggtaataaa atgtcgttaa ggttattggc taccgcccaa agaaaaaaca gtgagcacag
    12901 cagcgcgaat ggaataatgt aacttctgct ttgccctgca tctttatcta ccgcacggta
    12961 actctgcgtt tgtattgatg tgtttcccat agcatcctct taggttcaga agcttaagca
    13021 cgtaatcggt tatgttttca aaagtgcaaa accagcgagt tcgggcttca tatcagtgaa
    13081 tatttgcctg tctgaaatga actcatggat tcattttcaa ataaaaaacg ctctgtaata
    13141 tgacggcggt cacacttatt agctggaagg tgactttatg tgactaccat cactttaata
    13201 atcgaaattt catgcccgtt tcagaaaata aatcataaaa aacacaaacc agatgactgt
    13261 attaattgtt tatgccgaaa acgggcattc ctggcttggg tgaatagaaa aatgaccgtt
    13321 ttgataaata tttttaagag cggtcatttt taatggggtt ataaaaagag aacttgcagt
    13381 tatttactgt gattagttga gctaggtcac aaaatataag cttgtgaatt tctttcttgt
    13441 taatcaggaa gaagatggtg ggtaattaaa cggctaattc aatagtgtga aaggaacaac
    13501 attattgccc tgttttgaat cagagagagg tagctaatgg aacgaaataa acttgctcgt
    13561 cagattattg acacttgcct ggaaatgacc cgcctgggac tgaaccaggg gacagcgggg
    13621 aacgtcagtg tacgttatca ggatgggatg ctgattacgc ctacaggcat tccatatgaa
    13681 aaactgacgg agtcgcatat tgtctttatt gatggcaacg gtaaacatga ggaaggaaag
    13741 ctcccctcaa gcgaatggcg tttccatatg gcggcctatc aaagcagacc ggatgccaac
    13801 gcggttgttc acaatcatgc cgttcattgc acagcagttt ccattcttaa ccgaccgatc
    13861 cccgctattc actacatgat tgcggcggca ggtggtaatt ctattccttg cgcgccttat
    13921 gcgacctttg gaacacgcga actttctgaa catgttgcgc tggctctcaa aaatcgtaag
    13981 gcaactttgt tacaacatca tgggcttatc gcttgtgagg tgaatctgga aaaagcgtta
    14041 tggctggcgc atgaagttga agtgctggcg caactttacg tggcgaccct ggcgattacg
    14101 gacccggtgc cagtgctgag cgatgaagag attgccgtag tgctggagaa attcaaaacc
    14161 tatgggttac ggattgaaga gtaatttcgt aaagcaacaa ggagaaggat gatggctaac
    14221 agaatgattc tgaacgaaac ggcatggttt ggtcggggtg ctgttggggc tttaaccgat
    14281 gaggtgaaac gccgtggtta tcagaaggcg ctgatcgtca ccgataaaac gctggtgcaa
    14341 tgcggcgtgg tggcgaaagt gaccgataag atggatgctg cagggctggc atgggcgatt
    14401 tacgacggcg tagtgcccaa cccaacaatt actgtcgtca aagaagggct cggtgtattc
    14461 cagaatagcg gcgcggatta cctgatcgct attggtggtg gttctccaca ggatacttgt
    14521 aaagcgattg gcattatcag caacaatccg gagtttgccg atgtgcgtag cctggaaggg
    14581 ctttccccga ccaataaacc cagtgtaccg attctggcaa ttcctaccac agcaggtact
    14641 gcggcagaag tgaccattaa ctacgtgatc actgacgaag agaaacggcg caagtttgtt
    14701 tgcgttgatc cgcatgatat cccgcaggtg gcgtttattg acgctgacat gatggatggt
    14761 atgcctccag cgctgaaagc tgcgacgggt gtcgatgcgc tcactcatgc tattgagggg
    14821 tatattaccc gtggcgcgtg ggcgctaacc gatgcactgc acattaaagc gattgaaatc
    14881 attgctgggg cgctgcgagg atcggttgct ggtgataagg atgccggaga agaaatggcg
    14941 ctcgggcagt atgttgcggg tatgggcttc tcgaatgttg ggttagggtt ggtgcatggt
    15001 atggcgcatc cactgggcgc gttttacaac actccacacg gtgttgcgaa cgccatcctg
    15061 ttaccgcatg tcatgcacta taacgctgac tttaccggtg agaagtaccg cgatatcgcg
    15121 cgcgttatgg gcgtgaaagt ggaaggtatg agcctggaag aggcgcgtaa tgccgctgtt
    15181 gaagcggtgt ttgctctcaa ccgtgatgtc ggtattccgc cacatttgcg tgatgttggt
    15241 gtacgcaagg aagacattcc ggcactggcg caggcggcac tggatgatgt ttgtaccggt
    15301 ggcaacccgc gtgaagcaac gcttgaggat attgtagagc tttaccatac cgcctggtaa
    15361 atgcgctgat gtgataatgc cggatacgac gctagccgcg tcttatccgg tatataagtg
    15421 cattaaattc aatgtgttga taatcgtttg catccggctc gccgttaccg taccaaccgc
    15481 aattgctgta aattgccgtc gatatgcaaa tcggtttgta agcgggcaat atcgcggcac
    15541 agaaacgcca tctctttatg ggtttctaat tttttgcgcc acttttcggc aaccgcatcc
    15601 agattctcat atatcccttc cagactctga aactcgacca gcagctgcgt ggcgcttttt
    15661 ggtccgattc ccgcaacacc cggtaccttt gaactgctga tccccgcaag tccccagtaa
    15721 tcgggcaact gctgcggttg aacgccaaat tctttatcga taaatggcgc atccagccaa
    15781 cgtttctgga agtaatcacg aatacgtaat gtcggtgaaa gtaactgaca gtagccttta
    15841 tctgtcgaaa caatcgttgc ctgatgcccg gcctgtgtca ctttgaccgc cagcgtggcg
    15901 gctaagtcat cggcttcgtt gccgctggtt gaccagcacg ggacgccgcg ttgctcaaag
    15961 gcggcgcgta atgcaggcat ctcgtcgtgc aactcttccg gcattggcgg tcgacccgct
    16021 ttgtaatctg gtaaacgctg atgacgccag ccgctactgc ggttttcatc atcaaaaacg
    16081 gcgaccgcgt gggttggctg gctgtgcata atgagctgat cgagcgcatg ctggcaggtt
    16141 tcgacacagg gcgatccctg aacggcatga atgcgacgaa taagattcag tgcatcgaca
    16201 ataagcaaat gaacagccac gataaccccc ttaaagatca ggcgataagg gtaacattcg
    16261 atgccggaac aggctacggc tggatgagaa atcgggaaga ggcctcgcaa aacgaggcct
    16321 ctggagagcg attaatcgca ggcaacgatc ttcattgcca ggccgccgcg agaggtttcg
    16381 cggtacttgg cgttcatatc tttacctgtt tcgtacatgg tttcgataac tttatcgagg
    16441 cagacgcgcg gctcgctggt acggcgtagc gccatacgtg cggcgttcac cgcttttact
    16501 gccgcaatgg cgttacgctc gatgcatggc acctggacct gtccggcgac cgggtcacac
    16561 gtcagaccga ggttgtgctc catggcgatt tccgccgcga tgcacacctg cgccgggctt
    16621 gcacctaaca gttctgccag accagccgcc gccattgagc acgccacacc aacttcaccc
    16681 tggcagccga cttcagcacc agaaattgac gcgttcatct tataaagaga accaatggca
    16741 ctggctacca gcaggtaacg agccagtgag ttagcgttca cttcgcggat gaacttgtcg
    16801 tagtacgcca gcactgccgg gataatcccg cacgcaccgt tagtcggcgc agtcaccacg
    16861 cgtccgccag cagcgttctc ttcgttcact gccagtgcaa acatgttgat ccagtcaaca
    16921 accgccatcg ggtcagtggt ggttttatcc tggctgacca gcatccggcg tagtgccgca
    16981 gcacggcgtg gaacgcgcag tttgccaggc aacacgcctt cggtggaaat accgcgctca
    17041 ataccgccac gcatgacttc ccatacgttc gccaggtgct gttccagctc ttctttgctg
    17101 tgcagcgcca gctcattttt catcatcagg ccagagagtg acagcccggt ttcctgacaa
    17161 tgtttttgca gatcggctgc tgaactgtac ggataaggaa cttcaaccgg tgcgctattc
    17221 tgctggccaa aatgctcttc atcaacgata aagccaccgc caatagagta gtaagtctgg
    17281 ctgtaaacga ctttatcgcc cgccagcgcg gtaatgcgca taccgttttc atgcagagaa
    17341 aggttgtctg catggaagtt catgcactga tcaaccggga actccacttc atgctgaccg
    17401 tttgccagca tcaggcgacc atgagtattc acatcctgaa taaaaccagg gatggaatcg
    17461 atatccacgg tatccggcag gttacccgcc aggcccataa taatggcgat atcagtgtgg
    17521 tggcctttac cggtcagaga gagcgagccg tacacgtcaa ccaccacgcg ggtcacgtct
    17581 ttaagcagat tacgggcaat cagatcgtcg gtaaattgtt tacccgcttt cattggtcca
    17641 acggtatgag aactggaagg gccaatgccg attttgaaaa tatcgaatac gctaatcata
    17701 ggaaatacat cgtgttaaaa cggaggaagc gccgcccgaa agcggcgcga aaggacttag
    17761 ctgaacagag agtagaagat tgcggagatt gcaatcagac ccatcacgac aacgaatacg
    17821 ttgctgatgt gaccgctgta cttacgcatt gccggtactt tctgaattgc gtacatcggc
    17881 atcaggaaca ggatcatcgc gatgattggg ccgcccaggg tttcaatcat acccaggatg
    17941 ctcgggttca gggtggcaac aatccaggtc gttaccagca tgaacagcgc agtgatacgg
    18001 ttcagcttgt tgatttcgat agacttacct ttaccacgca gagatttaat caccataccg
    18061 ttgaagcctt cacgtgcgcc caggtagtgg ccgaggaagg atttggtgat agcgataatc
    18121 gcgataatcg gagccatcca cgcgataacc ggtgcgttaa agtggttagc caggtaagac
    18181 agaatcgaga tgttctgctc tttagccgca gccaggtctg ccggagtcag gctcagtaca
    18241 cagctgaaga cgaagaacat tacggtcagc accatcatga tgtgtgcgaa tgccaggatc
    18301 ttcgagcatt tctgttctgc catatcgccg tactcttcac gcttcgcaac ggcgaaagaa
    18361 gagatgatcg gagagtggtt gaaagagaac accataaccg gaattgccag ccacagggtc
    18421 atccacagac cgtttccggt tgcagatgca gtgtccagag acagcgtttc cagtgctgcg
    18481 ccgttccact gcgggatcag gtacagagcc agcagcatca gtacgccaac aaacgggaat
    18541 accagaatac tcattgcttt aacgatcatc tgctcaccga agcgaacgat ggtcatcata
    18601 cccacgatca ggatcagcga cagaatcgca cgcggcggtg gtgtcatacc cagctggtga
    18661 gacatgaagc tttcaactgt attggtgatt gccacgctat aaaccagcag gatcgggtag
    18721 atagcgaaga agtagagcag ggtaatcagt ttacctgcgc caataccaaa gtgttcttct
    18781 acaacctcgg tgatgtcttc gcctgggttt ttaccagaca gtacgaagcg agtcaggccg
    18841 cggtgagcaa aaaatgtcat cgggaacgca aggatagcca tgatgatcag cgggatcata
    18901 ccgccaacac cggcgttgat tggcaggaac agcacgcccg cgccgattgc cgtgccgtaa
    18961 aggcccagca tccacatggt gtctgtcttg cgccaggcac tacgagagtc tttcgacgca
    19021 atcgtgctgg tttgagtcgt ttccatctat ttctcctgga ggaatgcaaa taattcagct
    19081 tttagccagt tcagctcggg aaggtgcctg accgtaaaaa tgaaatctgt ctatcggcga
    19141 tggcttaatc atttaccgcc gtaataattt gagggcggaa agatacacac aagttatgta
    19201 tctatcagtg atcttgatcc caaatacaat aatctacttg gaagatagat tatcccaacg
    19261 ctttactctg taaatcaaaa gtaaaagaga atttaagggc aatatttgcc cattaaacga
    19321 catctgactt cttccggggc gcgcaatata tgtaggttga actgaaaaaa tcaatacttt
    19381 tagttaatca tggcgataat ttctttttca tagcgataaa atgtggttta tgaaaatagg
    19441 gaaacgtttg cgttacaaca taaattgtat gacatattaa ctgcaatgat atttatcgcg
    19501 tgagggtggt aagaaacatt catataacaa atgaaataaa ttgatgggta tgtgaccaaa
    19561 aacgagaaaa cccggcaaca aacgtcaccg ggggagggag attacgtgca gatttcgtag
    19621 caagggatgt aggctgagcc tggcaacttc atacgatgct gggcaacaaa accctgtagc
    19681 aggtcgtcca tacgacgcat aatctcttta tcgccgttga ttttgtaagg accaaactct
    19741 tcaatggcgc gaataccgac ttcttttacg ttacccgcca caataccgga gaacgcacgg
    19801 cgcagatcgg cagccagcac ttcaacaggt tgatccgggt aaagcttcag attagccata
    19861 ttctcgtgag acggctcaaa cggcatttgc aaatctggcg caatgcgcat tgaccagtta
    19921 aagctgtagg catcgcctgt gtcgcggcga ttttctttca ccagcggcat cgattttttc
    19981 atctgacgag cgacttcagc ggcgtcatca atgatgatgc ggtaatggcg gcgcgcgttt
    20041 tcacccaacg tatgcacgac aaactcgtcc agtacgcgga agtagtcggc gctctctttc
    20101 gggccggtga ggaacaatgg taaaacctga tctttgttgg ccgggttcat taaaattccc
    20161 agcaaataca gcaactcttc tgccgtaccc acaccgccag ggaagataat gatgccgtga
    20221 gcgatacgga caaacgcttc cagacgtttt tcgatatctg gcatgatgat caattcgttg
    20281 accagcgggt taggcggttc agcggcgata atcgacggct ctgtcatacc aataaaacga
    20341 ctgtctttgt aacgctgctg tgcgtgtccg accgcagcac ctttcatcgg cgcttccatc
    20401 gctcccggac cacagccggt gcagatattc agctcacgca ggcccagctg attcccgacg
    20461 cgacgggcat acaaatactc gttttcgtta attgagtgtc cgccccagca gaccaccata
    20521 tttggcgctt caccgacatg cagtgcgcga gcgttacgca agatggaaaa gaccaggtta
    20581 gtgatgtgga cggagttgtc gagattcagg ttgggaaaac gaacggtgtt atggatttgc
    20641 ccgtaaacga agagaatgtc acgcagaacc gcgaacaagt tggcctgcaa cgcgcgaata
    20701 attcgcccat cgacaaaagc ctcttccggg ggattaatca gttccagctt tacgccgcgt
    20761 tcacggcgca ggacgttaat atcgaaattt tcaaaacgag acagcaattc tttgctgtta
    20821 tcggtcaaac taccggagtt cagtacggca agtgaacagt tgcgaaacag ttgatagagg
    20881 tcgctgctgg cggtgcgttt aagcatatcc acttccagct gcgacaacat atccatggag
    20941 ccaagcgggc taatatgtgt aatcaagaaa aactccttat gggacgaata tcgtcattcc
    21001 ctttgattac aatagccctg cctgatgtcc tttcacaacc ttctgcgtga atccagcacg
    21061 caaaattgag aaaaaattta ttgccgaacc agtcttgtgg tcgatgggac aaaatcgcta
    21121 ttactgcgcc acgggttaat gtccagaccg ccacgacggg tataacgtgc gtaaacgctc
    21181 aatttttctg gctggcagaa gcgtaacagg tcattaaaga tgcgttccac gcactgttcg
    21241 tggaactcgt tgtgatgacg gaatgagacc aggtaacgca gcagtttttc tctgtcaatt
    21301 tggcgtccac gatactgaat ttggatcgaa ccccaatctg gttgatgggt gatcaggcag
    21361 tttgatttca gcaggtggct gacaagcgtc tcttccacta ctttttcacc acaggtggca
    21421 ttctccagat agtcagtagt gaattcatag ttatcgatag tgatatcctg gtcatcaatg
    21481 caagtgccat taaaatgacc tatcggctgg ccttccagtt catcaagacg atataacgcc
    21541 acgctaatct taccctgagc gcaagtgctt aagtcgcgct ccagcgtctg gcgcacctca
    21601 tcccagttat taaaacgcgt ctggttaaaa ctgttgagat agagcttaaa actcttcgac
    21661 tcaatcagat ttacgctggt gtaatcaagt tcaacatgac cgacagcgac ctgcggcaaa
    21721 cctttcgcat tcagccagga aagttcatac agcgtccaga tatccgtacc gtgaaaaggc
    21781 aggttatccg ctttcagacc cagcgggtcg cgattcaggc tgcgtggaac gccttgcagt
    21841 aggctggcgt cataggtatc ccggtaatcg gttgattttc caagagtcag gcccgcaagt
    21901 gcctgatggt ttgcataaga agacatgttt caccgtcata aaccagataa actaaacgac
    21961 aagtgtatct ggtttaagtg aagagagaaa ttagtggacg atttgaccgc acaagccctg
    22021 aaagatttta ctgcacgcta ctgcgatgcg tggcatgaag agcataaaag ctggccgtta
    22081 agtgaggaac tgtacggtgt tccttcaccg tgcattattt caactaccga agatgccgta
    22141 tactggcaac cccagccgtt taccggggaa caaaatgtaa acgcggttga acgcgctttt
    22201 gatattgtga tacaacccac aattcatacc ttttatacga ctcagtttgc cggggatatg
    22261 cacgcgcagt ttggcgatat caaactgaca ttgctgcaaa cctggagtga agacgacttc
    22321 cgtcgggtgc aggaaaatct gattggtcat ctggtgactc agaaaaggct aaaactgccg
    22381 cccacgctat ttatcgcgac gctggaagaa gagttagagg tcatctcggt gtgtaatctc
    22441 tccggtgaag tgtgcaaaga gacgctggga acacgtaaac ggacgcattt agcctcaaat
    22501 cttgcggaat tccttaatca acttaagcct cttctgtaat ccaaataccc catctggttg
    22561 tgagagatct cttacagacc ctgtaggaga tcgccaggaa ataagcgaat acttaaaaag
    22621 ataagaatcg ttattttcat ttaaaatcaa aatgttgatt gttaattctt aactttcata
    22681 tgaaattttc cttaaggcat tgtctgtaag cgccttgtaa gacttcgcga aaaagacgat
    22741 tctatcttcg tcgacaggga gtcagacaac gaagtgaaca tcaggatgat gacacttctg
    22801 caggacacac caggatggtg tttcagggaa aggcttctgg atgaagcgaa gaggatgacg
    22861 caggacgcgt taaaggacac ctccaggatg gagaatgaga accggtcagg atgattcggt
    22921 gggtcaggaa ggccagggac acttcaggat gaagtatcac atcggggtgg tgtgagcagg
    22981 aagcaatagt tcaggatgaa cgattggccg caaggccaga ggaaaagttg tcaaggatga
    23041 gcagggagca acaaaagtag ctggaatgct gcgaaacgaa ccgggagcac tgtgaataca
    23101 gtgctccctt tttttattcc tgctatcctt cgcggcagtt tttcttattg aagttgcttt
    23161 atgaccactc atgaccgcgt tcgtctccag ttgcagacgc ttgaagcgtt actgcgtgaa
    23221 catcagcact ggcgaaacga tgaaccccag ccgcatcaat ttaatagtac ccaaccgttc
    23281 tttatggaca ccatggaacc actggagtgg ttgcagtggg tgctgatccc gcgaatgcac
    23341 gatttgttaa ataacaacca gccgttaccg ggcgcttttg ctgttgcacc ctattatgaa
    23401 atggcgctgg cgacggacca tccgcaacgc gcgcttattc tggctgagtt ggaaaaactg
    23461 gatgctcttt ttgcggatga tgcgagctaa tgctggaaat actctatcag gatgaatggc
    23521 tggttgcggt aaataaaccc tccggctggc tggttcaccg tagctggctg gatcgcgacg
    23581 agaaagtagt ggtcatgcaa accgtgcgtg accagatagg acagcatgtt tttactgctc
    23641 atcgtctgga ccgacccact tctggtgtgt tgttgatggg attatccagc gaggccggac
    23701 ggctgctggc acaacagttt gaacagcacc aaatccagaa acgttaccat gcgattgtgc
    23761 gcggctggtt gatggaagaa gcggtgctgg attatccact ggtggaagaa ctggacaaaa
    23821 tcgctgataa atttgcccgc gaagataaag gcccgcagcc agcagtgacg cattatcgcg
    23881 gtctggcgac cgtagaaatg ccggtagcga ccggacgtta cccgaccacg cgctacggcc
    23941 tggtggaact ggagccgaaa accggacgca aacatcagct tcgccgacat cttgcccatt
    24001 tgcgtcatcc gattattggc gatagcaaac atggcgattt acgccagaat cgcagcggtg
    24061 ctgagcattt tggcctccag cggttaatgc tacatgccag tcagttgtca ttgacgcatc
    24121 cttttactgg cgagccgctg actattcacg cgggcctgga cgacacctgg atgcaggcgt
    24181 tatcacaatt tggctggcgc ggtctgcttc ctgaaaatga aagggttgag ttcagtgcgc
    24241 catcgggtca ggatggagag ataagttcct aatccaggga gaaaacggta tggcggaaat
    24301 tggtattttt gtcggcacca tgtacggaaa ttcgctgtta gtggcggaag aagcagaagc
    24361 aattctgacc gcacagggcc acaaagcaac ggtatttgaa gatcctgaat taagcgactg
    24421 gctgccttat caggataagt atgttctggt ggttacgtcc acgaccgggc agggcgacct
    24481 tcctgatagc attgtgccgc tctttcaggg aatcaaagat agtctgggct tccagccgaa
    24541 tctgcgttat ggcgtgattg cgctcggcga cagcagttat gtaaatttct gcaatggcgg
    24601 caaacagttc gatgccttgt tgcaggaaca gagcgctcag cgggttggcg aaatgctgtt
    24661 gattgacgcc agtgagaacc cggaaccgga aacggaatca aatccgtggg tcgaacagtg
    24721 gggcacgctg ttgtcctgat atgttcagcg agcggtaaat gtcgttttag cggtgctgaa
    24781 tcgaatcttt tcaggcaaat gccagtaaaa actgcttcat agcgcggatt tttactggcg
    24841 tttgcctgga gtcaagcgat ccatttcata ctcttcttta tttcttcgtt ttaacccttc
    24901 ctttcttgtt cttgttttca tttccgtgaa gtggattcca ccgtccaggg ctaatgccaa
    24961 aatcgggcct cattgaacgc attaatgttg tgttgttgca cggtgagccg ctatggcgcg
    25021 ctttttatac tgctattgcc agatataaac acgcgccgta ttcggcgaac gacctataaa
    25081 aacggcaaaa aacaccctac gtcacctctg atttcctggc tatgtcgcag tccagagtga
    25141 gcgtggctaa cgcgaatttt caggagtgca acaatgagtt ctttaagtca ggctgcgagc
    25201 agtgtggaaa aacgcacgaa tgctcgttac tggatagtgg tgatgttgtt tatcgtcaca
    25261 tccttcaact acggcgaccg cgctacgctt tctattgccg gttcggaaat ggccaaagat
    25321 atcggccttg atcccgtggg aatgggctat gtgttctctg ccttctcatg ggcttatgtt
    25381 atcgggcaga tccctggtgg ttggttgctg gatcgttttg gttcaaaacg cgtctacttc
    25441 tggtcgatct ttatctggtc gatgtttacc ttgctgcaag gcttcgtcga tatctttagt
    25501 ggattcggca ttatcgttgc cctgtttact ctgcgcttcc tggtcgggct tgctgaagcg
    25561 ccatctttcc ccggcaacag tcacattgtt gcggcctggt ttccggcgca ggaaagggga
    25621 acggcggtgt cgatttttaa ctccgctcaa tacttcgcaa cggtgatctt cgcgccgatt
    25681 atgggctggc tgacgcatga agtgggctgg tcacacgtct tcttctttat gggcggtctg
    25741 gggattgtca tcagctttat ctggttgaaa gtcatccacg agccaaatca acatccgggg
    25801 gtaaatcaga aagagctgga gtacatcgcc gcgggtggcg cgctgatcaa tatggatcag
    25861 caaaacacca aagttaaagt gccgttcagc gtgaagtggg ggcagatcaa acaactgctc
    25921 gggtcacgga tgatgatcgg cgtttatatc ggtcagtact gtatcaacgc cctgacttac
    25981 tttttcatta cctggtttcc ggtttatctg gtgcaggcgc gtgggatgtc gattctgaaa
    26041 gcgggctttg tggcttccgt tccggcggtt tgcggtttta tcggcggtgt gctgggtggg
    26101 attatttccg actggctgat gcgtcgcacg ggatcgctga acattgcgcg taaaacaccg
    26161 atcgtaatgg gcatgttgct gtcgatggtg atggtgttct gcaactacgt caacgttgag
    26221 tggatgatca tcggctttat ggcgctggcc ttcttcggta agggcatcgg ggcgctgggt
    26281 tgggcagtaa tggcagatac cgcgccaaaa gagatcagcg gtctttccgg tggcctgttc
    26341 aacatgttcg gtaacatttc tggcatcgtc acgccaatcg caattggtta tatcgttggc
    26401 acgactggct cctttaatgg ggcgctgatt tatgttggtg ttcatgcctt aatcgcggta
    26461 ctgagctacc tggtgctggt gggcgatatc aagcgtatcg agctgaaacc tgtcgcgggg
    26521 caataagatg acgacacaat ccagtcctgt tattactgat atgaaagtca ttccggtcgc
    26581 cgggcacgat agcatgttgc ttaatattgg tggggcacat aacgcatatt tcacccgcaa
    26641 tattgtggta ctcaccgata acgccgggca taccggcatt ggtgaagcgc cgggcggaga
    26701 ggtgatttat caaacactgg tcgatgctat tccgatggtt ctgggccagg aagttgcgcg
    26761 cctgaataaa gtggttcagc aggtgcataa aggtaatcag gcagccgatt ttgatacctt
    26821 cggcaaaggt gcctggactt ttgaattgcg cgttaatgcc gtggcggcgc tggaagccgc
    26881 cttgcttgac ctgctaggta aggcgctgaa tgttccggtc tgcgaactgt tggggccagg
    26941 caagcaacgc gagactatta ccgtcctcgg ttatctgttt tatatcggtg atcggaccaa
    27001 aaccgatctt ccttatctgg aaaatacgcc gggcaaccat gagtggtatc agttgcgcca
    27061 tcagaaagcg atgaacagcg aagccgttgt gcgtctggcg gaagcctcac aggatcgcta
    27121 tggctttaaa gatttcaaac ttaagggcgg ggtgttacct ggcgagcaag aaatcgacac
    27181 tgttcgtgca ttgaagaaac gcttccctga tgcgcggatt accgttgatc ccaacggtgc
    27241 ctggctactt gatgaagcca tttctctatg caaagggctg aatgatgttc ttacctatgc
    27301 cgaagatcca tgcggcgcag aacagggttt ctccggtcgt gaagttatgg cggagtttcg
    27361 gcgggcgacc ggcttgcccg tcgcgactaa catgatcgcc accaactggc gcgaaatggg
    27421 tcatgcggtg atgctcaatg cggtagatat tccacttgcc gatccgcact tctggacgct
    27481 ttccggtgca gtccgtgtgg cgcagctttg cgacgactgg gggctgacct ggggctgcca
    27541 ttctaataac catttcgata tctctctggc gatgtttacc catgtgggcg cggcggcacc
    27601 gggtaatcct accgctatcg atacccactg gatttggcag gagggcgatt gtcgcctgac
    27661 caaaaatccg ctggagatta aaaacggaaa aattgccgtt cctgatgcgc ccggtctggg
    27721 cgtggaactg gactgggaac aggtacaaaa ggcacatgag gcctataaac gtctgcctgg
    27781 cggtgcgcgt aacgacgcag gtccgatgca gtacctgatc cccggctgga cctttgaccg
    27841 taaacgtccc gttttcggcc gtcattgatt ctgaaaaagg acataaatat gagttctcaa
    27901 tttacgacgt ctgttgttac tgaaatgcag gtcatcccgg tggcgggtca tgacagtatg
    27961 ctgatgaatc tgagtggggc acacgcaccg ttctttacgc gtaatattgt gattatcaaa
    28021 gataattccg gtcacactgg cgtaggggaa attcccggcg gcgagaaaat ccgtaaaacg
    28081 ctggaagatg cgattccgct ggtggtaggt aaaacgctgg gtgaatacaa aaacgttctg
    28141 acgctggtgc gtaatacttt tgccgatcgt gatgctggtg ggcgcggttt gcagacattt
    28201 gatttgcgta ccactattca tgtagttacc gggatagaag cggcaatgct ggatctgctg
    28261 gggcagcatc tgggggtaaa cgtggcatcg ctgctgggcg atggtcaaca gcgtagcgaa
    28321 gtcgaaatgc tcggttatct gttcttcgtc ggtaatcgca aagccacacc tctgccgtat
    28381 caaagccagc cggatgactc atgcgactgg tatcgcctgc gtcatgaaga agcgatgacg
    28441 ccggatgcgg tggtgcgcct agcggaagcg gcgtatgaaa aatatggctt caacgatttc
    28501 aaactgaaag gcggtgtact ggccggggaa gaagaagccg agtctattgt ggcactggcg
    28561 caacgcttcc cgcaggcgcg tattacgctc gatcctaacg gtgcctggtc gctgaacgaa
    28621 gcaattaaaa ttggtaaata cctgaaaggt tcgctggctt atgcagaaga tccgtatggt
    28681 gcggagcaag gtttctccgg gcgtgaagtg atggcagagt tccgtcgcgc gactggcctg
    28741 ccgactgcaa ccaatatgat cgccaccgac tggcggcaga tgggccatac gctctcgtta
    28801 caatccgttg atatcccgct ggcggatccg catttctgga ctatgcaagg ttcggtacgt
    28861 gtggcgcaaa tgtgccatga atttggcctg acctggggtt cacactcaaa caaccacttc
    28921 gatatttccc tggcgatgtt tacccatgtt gccgccgctg caccgggtaa aatcaccgcg
    28981 attgatacgc actggatttg gcaggaaggc aatcagcgct tgaccaaaga accgtttgag
    29041 atcaaaggcg gtctggttca ggtgccggaa aaaccggggc tgggtgtaga aatcgatatg
    29101 gatcaagtga tgaaagccca tgagctgtat cagaaacacg ggcttggcgc gcgtgacgat
    29161 gcgatgggaa tgcagtatct gattcctggc tggacgttcg ataacaagcg cccgtgcatg
    29221 gtgcgttaag acagaaaacc aaaaccggct ctatctggag ccggcttgag actgatgcta
    29281 aacgcaaaat agcctgttgt gctatgtatc aggtctacat cattttttaa ttcattgaat
    29341 ttaattggtt ttgtaggccg gataaggcgt taacgccgca tccggcataa acacaggcac
    29401 tttgtcacca atctgaaacc agcgtcataa aaagccgatt gctactcgac aagacgtccg
    29461 ttacccgaga attttgctgg cttcgcgcgc gacattatcc atctcgtcca acagttccag
    29521 cagctccggc tccaaatctt cttctttagt accgctacgt agctgttgtt cgatcagttg
    29581 gcagagattc ttcatacgcg gcacaccgct atagccgcaa ctgccatgca gtttatgaat
    29641 caaatcaacc aggccttccg ggttttctcc aaccagctgc tcctcaactt tgttgcgaac
    29701 ttcaggcagg aaatcgagta acatttgcag catatcgcgc gctagatcgg tttttcctgc
    29761 tgcctggcgt agtgccagtt gccagtcgag ggtcgcattc gggttcacca caatttcgtt
    29821 gacttcgggc gtcacgacgc gagaggaaat accgctgcca ggcttgtagc gcaacaacaa
    29881 attatgcaat cgctcttctt caatcggttt cgccagataa tcgctcatcc ctgcgccaag
    29941 cagcttctct ttttgcccgg ccattgcatg cgccgttacc gcgataaccg gcgtttgttg
    30001 ctgatggggg agctggtgga tgagctcgca ggcccgaatg ccatccatgt caggcatttg
    30061 aatatccatt aagatcaaat cgaacggcat ctgtttcgcc cgttcaaccg cctgatgccc
    30121 gctatcgcaa agttccacat gttgcaccat atcttccagc aatgcgccga taagtttcag
    30181 gttagcgggg ttgtcatcaa ctgccatgac tgtcattgcc agcttgcttt catcggttac
    30241 aggcaaaagc gtgttttgtt tgtgatgaca aaattccgtc agggcaggca acaggcgcgt
    30301 aggtgttaat ggtttcagca gacacgcgcc gataccatct tgcttgagtt tttctgcatt
    30361 gacttgtgca tggcaaggaa gtgccagcat caggaaatcg gtcatcgata ccgctttcgc
    30421 taatcgctca tgttgcattg ttagcggctc gcggaaggtc accgcgatgc ctaacaacat
    30481 catgtcgtaa tgcgcgggag gcagcgcgga gaacgttggg ctataaacca cttccagcgg
    30541 cgtttcactt aaaatatcca gcgtgcattg cgctgctgcg gagtttggtt cgacataggc
    30601 caggcgctta ccggcgaggc actgggtgga tggcccttcg ataataatgt tcgggttcag
    30661 atcgagatta atgtggaacc agaaagttga accgcgattc ggctggctat ggaacgaaat
    30721 atcgccgccc atttcattaa ccagtttttg tgtaatcacc agccccagac cggtgccacc
    30781 atgacggcgg gaaatactgg catcagcctg tcggaaggcc tggaataagc gcgattgatc
    30841 gcgttcagga ataccaatgc cggtatcccg aatctgcact tcaatctgca ctttggtatt
    30901 actcagcgca cgtttttcta ccagaatatc aatgttgcca ttctcggtga atttaattgc
    30961 attccccacc aggttagtga tgatttgctg taatcgcagt gggtcgccga tcacgttatc
    31021 aggcacgtcg cttttaatat tgagcgtcag ttccaaccct ttatcgtgag aagaatgtgc
    31081 cagcagagta acgacttcat ccagcgtgct gcgtagtggg aatggaatac tttccagaat
    31141 cagcttacct gcttccagtt tcgagaagtc gagaacatca ttaataattg ccagcaaatt
    31201 atttgccgaa cgttcaatcg tattcaggtg atcgcgctgc gttggtgtta attctgtttt
    31261 cagcgtcagg cgggtaaagc caataacacc attcagtggt gtacgcagct cgtgtgacat
    31321 atttgccaga aactcggatt taatacgcgc cgcttcctgg gcgcgctttt tcgccagatc
    31381 taactcaacg ttctgaattt ccatctgctc cagcgtttca cgcagatcgg acgtcgcctg
    31441 gtcgatattg tgctgcatct cttcgtgata agcagccagc gacattgcca tcgagttgat
    31501 accgtttttc agcatatcca gctcgccgag cataaatcct tccacccggc tgtcgagttg
    31561 cccgcgacgg atgcggtcga cggtattcac catgttgcga atcggaccgg ttacatcgcg
    31621 cattaagcgc cagccaaaaa taagcgcaat accgatacaa aacagcatca tcacgctgga
    31681 aataaagatc tctttatatt gctgcaagcg aaccgattta agatccagct ccagcgcaat
    31741 atatcccaac atattttgac tatttttggc atcgctactg ggcgattcgt cgggggagta
    31801 actctcagaa ataatcggcg tgcggaggat cataatatcg ccgtcacgag tgacagtgag
    31861 ctggcgagga aacggcacgt tgctgccgag ctgcattgat gagggatcaa gatgaaaatt
    31921 ggaggtgaca aagagtcggt tattttcatc ataaaccgaa atcgcgcgaa caatatcgga
    31981 atggcgacga tgcagtacgc ttattaactg accgatagat tcgcgatttt gcaggctcat
    32041 gccatattca gtagaaactg caagcggctc aataatgctg gctccggcat cttccagttg
    32101 acgctgcaag tcgttatagc gatgcacaac gaaaaagata ctcagcaata aaccaataag
    32161 gacggtcggt gccaggatca gaatcatcat gcgtgcgcgc aggctgtagt tggtcatgga
    32221 gttccgttat gggacaatta aggtcacact gttaaattga gaaagtctcg ccaatggcgc
    32281 aattctactc tgcaaaacga cgcacgacga cgcgtcagat cataaccgtt tcagtcaacg
    32341 acctcgactc ttttggtcag ggcgtggcgc gacataacgg caaaacgcta tttatccccg
    32401 gattattgcc gcaggaaaac gcggaagtta ctgttactga agataaaaaa cagtatgccc
    32461 gcgctaaagt cgtacgccgg ttaagcgata gcccgggacg cgaaacgcca cgctgtcctc
    32521 attttggcgt atgcggcggc tgtcagcaac aacacgccag cgtggattta cagcagcgaa
    32581 gcaaaagtgc ggcactcgcc cgattaatga aacacgaagt ctctgaagtg atcgccgatg
    32641 ttccctgggg ctatcgccgt cgcgcgcgtt taagtctgaa ctacttaccg aaaacacagc
    32701 aacttcagat ggggtttcgc aaagcgggct ccagtgacat tgtcgacgtt aaacaatgcc
    32761 ccattttagt gccccaactt gaagcattgc tgcccaaagt catggcatgc ttgggcagct
    32821 tacaagttat gcgccatctt ggtcatgttg aactggtaca ggcaaccagc ggcacgctga
    32881 tgattttgcg ccataccgca ccgctaagtt cggcagatcg cgaaaaactg gaacgctttt
    32941 cgcattctga aggcctggat ctgtatctcg cccccgatag tgagatactc gaaaccgtct
    33001 ctggtgagat gccctggtat gactcaaatg ggttgcgctt aacttttagc ccgcgcgatt
    33061 ttattcaggt caatgcgggt gtgaaccaaa aaatggtagc gcgtgcgttg gaatggctgg
    33121 atgtgcaacc tgaagatcgc gtactggatt tgttctgcgg tatgggcaac tttacactgc
    33181 cattggcgac acaagctgcc agtgtggtgg gtgtagaagg cgttccggcg ctggtggaaa
    33241 aaggccagca gaatgcgcgt cttaatggct tacagaatgt gacgttttat cacgaaaatc
    33301 ttgaagaaga tgtcacaaag cagccgtggg cgaaaaacgg cttcgataaa gtgttgctgg
    33361 acccggcgcg agcaggtgcc gcaggtgtta tgcagcaaat tataaaactg gaacctattc
    33421 gtatagttta tgtatcctgt aaccctgcaa cgctggctcg ggatagcgaa gcgttattaa
    33481 aagcaggata taccattgcg cgactggcga tgctggatat gttcccacac acgggacatc
    33541 tggaatcgat ggtacttttc tcgcgcgtta aatagttgcg atttgccgat ttcggcaggt
    33601 ctggtcccta aaggagagga cgatggttgc ggtaagaagt gcacatatca ataaggctgg
    33661 tgaatttgat ccggaaaaat ggatcgcaag tctgggtatt accagccaga agtcgtgtga
    33721 gtgcttagcc gaaacttggg cgtattgtct gcaacagacg caggggcatc cggatgccag
    33781 tctgttattg tggcgtggtg ttgagatggt ggagatcctc tcgacattaa gtatggacat
    33841 tgacacgctg cgggcggcgc tgctgttccc tctggctgat gccaacgtag tcagcgaaga
    33901 tgtgctgcgt gagagcgtcg gtaagtcggt tgttaacctt attcacggcg tgcgtgatat
    33961 ggcggcgatc cgccagctga aagcgacgca cactgattct gtttcctccg aacaggtcga
    34021 taacgttcgc cggatgttat tggcgatggt cgatgatttt cgctgcgtag tcatcaaact
    34081 ggcggagcgt attgctcatc tgcgcgaagt aaaagatgcg ccggaagatg aacgtgtact
    34141 ggcggcaaaa gagtgtacca acatctacgc accgctggct aaccgtctcg gaatcggaca
    34201 actgaaatgg gaactggaag attactgctt ccgttacctc catccaaccg aatacaaacg
    34261 aattgccaaa ctgctgcatg aacggcgtct cgaccgcgaa cactacatcg aagagttcgt
    34321 tggtcatctg cgcgctgaga tgaaagctga aggcgttaaa gcggaagtgt atggtcgtcc
    34381 gaaacacatc tacagcatct ggcgtaaaat gcagaaaaag aacctcgcct ttgatgagct
    34441 gtttgatgtg cgtgcggtac gtattgtcgc cgagcgttta caggattgct atgccgcact
    34501 ggggatagtg cacactcact atcgccacct gccggatgag tttgacgatt atgtcgctaa
    34561 cccgaaacca aacggttatc agtctattca taccgtggtt ctggggccag gtggcaaaac
    34621 cgttgagatc caaatccgca ccaaacagat gcatgaagat gcagagttgg gtgttgctgc
    34681 gcactggaaa tataaagagg gcgcggcagc tggcggcgca cgttcgggac atgaagaccg
    34741 gattgcctgg ctgcgtaaac tgattgcgtg gcaggaagag atggctgatt ccggcgaaat
    34801 gctcgacgaa gtacgcagcc aggtctttga cgaccgggtg tatgtcttta cgccgaaagg
    34861 tgatgtcgtt gatttgcctg cgggatcaac gccgctggac ttcgcttacc acatccacag
    34921 tgatgtcgga caccgctgca tcggggcaaa aattggcggg cgcattgtgc cgttcaccta
    34981 ccagctgcag atgggcgacc agattgaaat tatcacccag aaacagccga accccagccg
    35041 tgactggtta aacccaaacc tcggttacgt cacaaccagc cgtgggcgtt cgaaaattca
    35101 cgcttggttc cgtaaacagg accgtgacaa aaacattctg gctgggcggc aaatccttga
    35161 cgacgagctg gaacatctgg ggatcagcct gaaagaagca gaaaaacatc tgctgccgcg
    35221 ttacaacttt aatgatgtcg acgagttgct ggcggcgatt ggtggcgggg atatccgtct
    35281 caatcagatg gtgaacttcc tgcaatcgca atttaataag ccgagtgccg aagagcagga
    35341 cgccgccgcg ctgaagcaac ttcagcaaaa aagctacacg ccgcaaaacc gcagtaaaga
    35401 taacggtcgc gtggtagtcg aaggtgttgg caacctgatg caccacatcg cgcgctgctg
    35461 ccagccgatt cctggagatg agattgtcgg cttcattacc caggggcgcg gtatttcagt
    35521 acaccgcgcc gattgcgaac aactggcgga actgcgctct catgcgccag aacgcattgt
    35581 tgacgcggta tggggtgaga gctactccgc cggatattcg ctggtggtcc gcgtggtggc
    35641 taatgatcgt agtgggttgt tacgtgatat cacgaccatt ctcgccaacg agaaggtgaa
    35701 cgtgcttggc gttgccagcc gtagcgacac aaaacagcaa ctggcaacca tcgacatgac
    35761 cattgagatt tacaacctgc aggtgctggg ccgcgtgctg ggtaaactca accaggtgcc
    35821 ggatgttatc gacgcgcgtc ggttgcacgg gagttaggca gaaatttgct cgtatctaca
    35881 atgtagattg atatatactg tatctacata tgatagcggt ttgaggaaag ggttatgatc
    35941 cacagtagcg taaagcgttg gggaaattca ccggcggtgc ggatcccggc tacgttaatg
    36001 caggcgctca atctgaatat tgatgatgaa gtgaagattg acctggtgga tggcaaatta
    36061 attattgagc cagtgcgtaa agagcccgta tttacgcttg ctgaactggt caacgacatc
    36121 acgccggaaa acctccacga gaatatcgac tggggagagc cgaaagataa ggaagtctgg
    36181 taatggtaag ccgatacgta cccgatatgg gcgatctgat ttgggttgat tttgacccga
    36241 caaaaggtag cgagcaagct ggacatcgtc cagctgttgt cctgagtcct ttcatgtaca
    36301 acaacaaaac aggtatgtgt ctgtgtgttc cttgtacaac gcaatcaaaa ggatatccgt
    36361 tcgaagttgt tttatccggt caggaacgtg atggcgtagc gttagctgat caggtaaaaa
    36421 gtatcgcctg gcgggcaaga ggagcaacga agaaaggaac agttgcccca gaggaattac
    36481 aactcattaa agccaaaatt aacgtactga ttgggtagtg ttactaactc acttctattc
    36541 tggtcacagg tttccacctg acagacccgc aattttcagg acaattcaat gaatcaaatc
    36601 gaccgtttgc tcactattat gcagcgcctg cgcgatccgg aaaacggctg cccgtgggat
    36661 aaagagcaga catttgccac cattgcgcct tacacccttg aagaaaccta cgaagtgctg
    36721 gacgccatcg cccgtgaaga ttttgacgat cttcgcggtg aactgggcga tctgctattc
    36781 caggtggtgt tttacgcgca aatggctcag gaagaagggc gctttgactt taatgatatt
    36841 tgcgctgcta ttagcgataa attagagcgt cgccatccgc atgtttttgc tgatagttct
    36901 gccgaaaaca gtagtgaagt gcttgcccgt tgggagcaaa tcaaaaccga agagcgcgcg
    36961 cagaaagcgc agcattcggc gctggacgat attcctcgta gtttaccggc tttaatgcgg
    37021 gcgcaaaaaa tccagaaacg ttgcgccaac gttggcttcg actggacgac gcttggcccg
    37081 gtggttgata aagtctacga agagatcgac gaggtgatgt acgaggcgcg gcaggctgtt
    37141 gtcgaccagg ctaaactgga ggaggaaatg ggggacctgc tgtttgccac ggttaatctg
    37201 gctcgccatt tagggacgaa agcagaaatc gcattgcaaa aagcgaacga aaaattcgag
    37261 cgtcgttttc gcgaagtgga gcgtattgtt gccgcgcgtg gactggaaat gacaggtgtt
    37321 gacctcgaaa caatggaaga agtctggcaa caggtaaaac ggcaggaaat tgatctctaa
    37381 gggaattacg cggtcaagcg ccatttgtgt cattttttaa atgacaagcg cttgatttgc
    37441 gtcaaaaaca tttaccccaa aggggctatt ttctcactcc tgatttcaat agtgcgctgg
    37501 cgaagaggag ggataatgaa agtttgtggc acaggtcatg ttcgggtata ctgctttccc
    37561 gtcctggtta ttccatcgtc ttttcaacct aacttctcag gttcagcatg acaacgaact
    37621 atatttttgt gaccggcggg gtcgtatcct ctctgggtaa aggcattgcc gcagcctccc
    37681 tcgcagccat tcttgaagcc cgtggcctca atgtgaccat catgaaactg gatccgtaca
    37741 tcaacgtcga tccaggtact atgagcccaa tccaacacgg ggaagtgttc gttactgaag
    37801 acggcgctga aaccgacctg gacctggggc actacgagcg tttcattcgt accaaaatga
    37861 gccgccgcaa caacttcacc acgggtcgta tctactctga cgttctgcgt aaagaacgcc
    37921 gcggtgacta cctcggcgca accgtgcagg ttattccgca catcactaac gcaatcaaag
    37981 agcgcgtgct ggaaggtggc gaaggtcatg acgtagtact ggtagaaatc ggcggtacag
    38041 taggtgatat cgaatccttg ccgttcctcg aagcgattcg ccagatggct gttgaaattg
    38101 gccgtgagca cactctgttt atgcacctga cgctggtgcc gtacatggca gcgtctggtg
    38161 aagtcaaaac caaaccgact cagcactctg taaaagagct gctctccatc ggtatccagc
    38221 ctgacatcct gatttgtcgt tcagatcgcg ctgttccggc gaacgaacgt gcgaagattg
    38281 cattgttctg taatgttccg gaaaaagcgg ttatttctct gaaagacgtc gattccatct
    38341 ataaaattcc gggcctgttg aaatctcagg ggctggacga ttatatttgt aaacgattca
    38401 gcttaaactg cccggaagca aatctgtccg aatgggaaca ggttatcttc gaagaagcga
    38461 atccggtaag tgaagtcacc atcggtatgg tcggcaagta cattgaactg ccggatgctt
    38521 ataaatcagt gatcgaagca ctgaaacacg gtgggctgaa gaatcgtgtc agcgtcaaca
    38581 tcaaactgat cgattcacaa gatgttgaaa cgcgcggcgt tgaaatcctt aaaggtctgg
    38641 acgcaatcct cgtacctggc ggtttcggct atcgtggcgt agaaggcatg attacgaccg
    38701 cgcgttttgc gcgtgagaac aatattcctt atctgggcat ttgcctgggt atgcaggtgg
    38761 cgttaattga ttacgctcgc catgttgcca acatggagaa cgccaactct acggaatttg
    38821 tgccagactg taagtacccg gttgtggcgc tgattaccga gtggcgcgat gaaaacggca
    38881 acgttgaagt tcgtagcgag aagagcgatc tcggcggtac catgcgtctc ggcgcacagc
    38941 agtgccagtt ggttgacgat agcctggttc gccagctgta caatgcgccg acaattgttg
    39001 agcgtcatcg tcaccgttac gaagtcaaca acatgctgtt gaaacagatt gaagatgcag
    39061 gtctgcgcgt tgcgggccgt tccggggatg atcagttggt cgagatcatc gaagttccga
    39121 atcacccgtg gttcgtggct tgccagttcc atccggagtt tacttctact ccacgtgatg
    39181 gtcacccgct gtttgcaggc tttgtgaaag ctgccagcga gttccagaaa cgtcaggcga
    39241 agtaagtaaa aaagttagaa cggcaacgcg taccttgggt acgcgttgtt tgtctggagt
    39301 ttcagtttaa ctagtgactt gaggaaaacc taatgtccaa aatcgtaaaa atcatcggtc
    39361 gtgaaatcat cgactcccgt ggtaacccga ctgttgaagc cgaagtacat ctggagggtg
    39421 gtttcgtcgg tatggcagct gctccgtcag gtgcttctac tggttcccgt gaagctctgg
    39481 aactgcgcga tggcgacaaa tcccgtttcc tgggtaaagg cgtaaccaaa gctgttgctg
    39541 cggtaaacgg cccgatcgct caggcactga ttggcaaaga cgctaaagat caggctggca
    39601 ttgacaagat catgatcgac ctggacggca ccgaaaacaa atccaaattc ggcgcgaacg
    39661 caatcctggc tgtatctctg gctaacgcca aagctgctgc tgccgataaa gggatgccgc
    39721 tgtacgagca catcgctgaa ctgaacggca ctccgggcaa atactccatg ccggttccga
    39781 tgatgaacat catcaacggt ggtgagcacg ctgacaacaa cgttgatatc caggaattca
    39841 tgattcagcc ggttggcgcg aaaactgtga aagaagccat ccgcatgggt tctgaagttt
    39901 tccatcacct ggcaaaagtt ctgaaagcga aaggcatgaa cactgcagtt ggtgacgaag
    39961 gtggctatgc gccgaacctg ggttccaacg ctgaagctct ggctgttatc gctgaagctg
    40021 ttaaagctgc tggttatgaa ctgggcaaag acatcactct ggcgatggac tgcgcagctt
    40081 ctgaattcta caaagacggt aaatacgttc tggctggcga aggcaacaaa gctttcacct
    40141 ctgaagaatt cactcacttc ctggaagaac tgaccaaaca gtatccgatc gtatctatcg
    40201 aagacggtct ggacgaatct gactgggacg gtttcgcata ccagaccaaa gttctgggcg
    40261 acaaaatcca gctggttggt gacgacctgt tcgtaaccaa caccaagatc ctgaaagaag
    40321 gtatcgaaaa aggtatcgct aactccatcc tgatcaaatt caaccagatc ggttctctga
    40381 ccgaaactct ggctgcaatc aagatggcga aagatgctgg ctacactgca gttatctctc
    40441 accgttctgg cgaaactgaa gacgctacca tcgctgacct ggctgttggt actgctgcag
    40501 gccagatcaa aactggttct atgagccgtt ctgaccgtgt tgctaaatac aaccagctga
    40561 ttcgtatcga agaagctctg ggcgaaaaag caccgtacaa cggtcgtaaa gagatcaaag
    40621 gccaagcata agactgactt tatctgattt aaaaatgcca gcctc
//
LOCUS       DAJTYZ010000020        39788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000020 DAJTYZ010000000
VERSION     DAJTYZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.020"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            4..435
                     /locus_tag="OO596_001050"
     CDS             4..435
                     /locus_tag="OO596_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6716967.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="OO596_001051"
     CDS             518..1420
                     /locus_tag="OO596_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR6716968.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OO596_001052"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OO596_001052"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6716969.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OO596_001053"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OO596_001053"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6716970.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OO596_001054"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OO596_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6716971.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="OO596_001055"
     CDS             complement(5330..6037)
                     /locus_tag="OO596_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6716972.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OO596_001056"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OO596_001056"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6716973.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="OO596_001057"
     CDS             complement(6895..7098)
                     /locus_tag="OO596_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6716974.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7610..7930
                     /gene="cyaY"
                     /locus_tag="OO596_001058"
     CDS             7610..7930
                     /gene="cyaY"
                     /locus_tag="OO596_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6716975.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7970..10516)
                     /gene="cyaA"
                     /locus_tag="OO596_001059"
     CDS             complement(7970..10516)
                     /gene="cyaA"
                     /locus_tag="OO596_001059"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6716976.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10903..11844
                     /gene="hemC"
                     /locus_tag="OO596_001060"
     CDS             10903..11844
                     /gene="hemC"
                     /locus_tag="OO596_001060"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6716977.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11841..12581
                     /gene="hemD"
                     /locus_tag="OO596_001061"
     CDS             11841..12581
                     /gene="hemD"
                     /locus_tag="OO596_001061"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6716978.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            12603..13814
                     /gene="hemX"
                     /locus_tag="OO596_001062"
     CDS             12603..13814
                     /gene="hemX"
                     /locus_tag="OO596_001062"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6716979.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            13817..15013
                     /gene="hemY"
                     /locus_tag="OO596_001063"
     CDS             13817..15013
                     /gene="hemY"
                     /locus_tag="OO596_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6716980.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15675..15836
                     /locus_tag="OO596_001064"
     CDS             15675..15836
                     /locus_tag="OO596_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6716981.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15920..17155)
                     /locus_tag="OO596_001065"
     CDS             complement(15920..17155)
                     /locus_tag="OO596_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6716982.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17302..17378)
                     /locus_tag="OO596_001066"
     tRNA            complement(17302..17378)
                     /locus_tag="OO596_001066"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17342..17344),aa:Pro,seq:tgg)
     gene            complement(17421..17507)
                     /locus_tag="OO596_001067"
     tRNA            complement(17421..17507)
                     /locus_tag="OO596_001067"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17471..17473),aa:Leu,seq:cag)
     gene            complement(17528..17603)
                     /locus_tag="OO596_001068"
     tRNA            complement(17528..17603)
                     /locus_tag="OO596_001068"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17568..17570),aa:His,seq:gtg)
     gene            complement(17662..17738)
                     /locus_tag="OO596_001069"
     tRNA            complement(17662..17738)
                     /locus_tag="OO596_001069"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17702..17704),aa:Arg,seq:ccg)
     gene            complement(17839..19224)
                     /locus_tag="OO596_001070"
     CDS             complement(17839..19224)
                     /locus_tag="OO596_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6716983.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19415..20155)
                     /gene="rffM"
                     /locus_tag="OO596_001071"
     CDS             complement(19415..20155)
                     /gene="rffM"
                     /locus_tag="OO596_001071"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6716984.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20158..21510)
                     /gene="wzyE"
                     /locus_tag="OO596_001072"
     CDS             complement(20158..21510)
                     /gene="wzyE"
                     /locus_tag="OO596_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6716985.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21507..22586)
                     /gene="rffT"
                     /locus_tag="OO596_001073"
                     /gene_synonym="wecF"
     CDS             complement(21507..22586)
                     /gene="rffT"
                     /locus_tag="OO596_001073"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6716986.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22583..23833)
                     /gene="wzxE"
                     /locus_tag="OO596_001074"
     CDS             complement(22583..23833)
                     /gene="wzxE"
                     /locus_tag="OO596_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6716987.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23835..24965)
                     /gene="rffA"
                     /locus_tag="OO596_001075"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23835..24965)
                     /gene="rffA"
                     /locus_tag="OO596_001075"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6716988.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24970..25644)
                     /gene="rffC"
                     /locus_tag="OO596_001076"
                     /gene_synonym="wecD"
     CDS             complement(24970..25644)
                     /gene="rffC"
                     /locus_tag="OO596_001076"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6716989.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25622..26503)
                     /gene="rfbA"
                     /locus_tag="OO596_001077"
     CDS             complement(25622..26503)
                     /gene="rfbA"
                     /locus_tag="OO596_001077"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6716990.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26522..27589)
                     /gene="rffG"
                     /locus_tag="OO596_001078"
     CDS             complement(26522..27589)
                     /gene="rffG"
                     /locus_tag="OO596_001078"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6716991.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27589..28851)
                     /gene="wecC"
                     /locus_tag="OO596_001079"
     CDS             complement(27589..28851)
                     /gene="wecC"
                     /locus_tag="OO596_001079"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6716992.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28848..29978)
                     /gene="wecB"
                     /locus_tag="OO596_001080"
     CDS             complement(28848..29978)
                     /gene="wecB"
                     /locus_tag="OO596_001080"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6716993.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30034..31080)
                     /gene="wzzE"
                     /locus_tag="OO596_001081"
     CDS             complement(30034..31080)
                     /gene="wzzE"
                     /locus_tag="OO596_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6716994.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31092..32195)
                     /gene="wecA"
                     /locus_tag="OO596_001082"
     CDS             complement(31092..32195)
                     /gene="wecA"
                     /locus_tag="OO596_001082"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6716995.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32435..33694)
                     /gene="rho"
                     /locus_tag="OO596_001083"
     CDS             complement(32435..33694)
                     /gene="rho"
                     /locus_tag="OO596_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6716996.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33779..33880)
                     /gene="rhoL"
                     /locus_tag="OO596_001084"
     CDS             complement(33779..33880)
                     /gene="rhoL"
                     /locus_tag="OO596_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6716997.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34021..34350)
                     /gene="trxA"
                     /locus_tag="OO596_001085"
     CDS             complement(34021..34350)
                     /gene="trxA"
                     /locus_tag="OO596_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6716998.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34481..35746
                     /gene="rhlB"
                     /locus_tag="OO596_001086"
     CDS             34481..35746
                     /gene="rhlB"
                     /locus_tag="OO596_001086"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6716999.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35880..37364
                     /gene="gppA"
                     /locus_tag="OO596_001087"
     CDS             35880..37364
                     /gene="gppA"
                     /locus_tag="OO596_001087"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6717000.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37411..39432)
                     /gene="rep"
                     /locus_tag="OO596_001088"
     CDS             complement(37411..39432)
                     /gene="rep"
                     /locus_tag="OO596_001088"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6717001.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9866 a        10817 c        10042 g         9063 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg ctttctgctt
     7141 tctgctttct gctttctgct ttctgctttc tgctttctgc tttctatcat cgcaggagaa
     7201 gacgtaaaag caagcgttta gcgactcttt atgaagtctg aaaagtgagg aaatgctgcg
     7261 agtataacgc ttactcgtgg ggatttatgt tttgctggta gtttcgtgat cggcacctcg
     7321 cgccaggatg gctctatgca ctatcgcaat gtggtttgtt tgttgtcttt tttagttccg
     7381 tcagcgtgag gatgctcgct cggtaaacct gatcataatg tttaccagaa acagggaaag
     7441 gcgtggtgta actgcgccct aatgaagaga caaatctttc gctacacaga gacatcatta
     7501 ttccggtgac ggatgaatcg tgccggaacg cagccgcaga ggtgcaaact ttgaagccct
     7561 gaccccatgc cgctatactg ccgccatcac ttaaaaacag gatacaacaa tgaacgacag
     7621 tgaatttcat cgcctggctg atcaactctg gctgaccatt gaagaacgcc tggacgactg
     7681 ggatggcgac agcgatatcg actgcgaaat caacggcggc gtgctgacca ttacctttga
     7741 aaacggcagc aaaatcatta tcaaccgcca ggaaccgctg caccaggtat ggctggcaac
     7801 caaacaaggc ggctaccatt ttgatctgaa aggcgatgag tggatttgcg atcgcagcgg
     7861 cgaaaccttc tgggatttgc tggaacaggc ggcgacgcag caggcgggtg aaacagtcag
     7921 tttccgctaa gattgcatgc cggataagcc tcgctttccg gcacgttcat cacgaaaaat
     7981 attgctgtaa tagcggcgta tcgtgatcct gattggcagg cggcatgtta ccgatagatt
     8041 ttgtgcggaa cggaatcacc tgttcacgac catcaacctt cacaatctga tagaactgcg
     8101 gcaggttgaa gttgatgaag cttgagccgt aggtgaagcg gtcgtgcgat gacgagtaga
     8161 agcgactgac gtcacgcacc agttcctctt tgctgccttc gcagtggtga tacacctcaa
     8221 cccggttgct ttcgtcaaga atgtagatat taaagccatt ctcgtcttgc gtttcttcga
     8281 agaagaactg gatgatccct tcactagcaa agccgtccac caccgccggt aatttgacgt
     8341 gattggtttc aacctgcact gacaggccgt gcagtttgtt atgcgaaata gcgccataaa
     8401 actcgatggc gttctccagc ttctgtaccg atacattcag gcgttcgaag aacaagcccc
     8461 aggtttgacc agaaacgcgc agcgccttga aacgcccggt ttcctggcgg gtgctggaaa
     8521 ggcgcagttc aatacactca gaaaccagtt gctgcacgcg agtacggatt aacccgcgca
     8581 gatgctggct ataacagaag acttccacgc tatctggcgg tgcggcgtcc tgatgcattt
     8641 tgccgagaat agttttcagg gcttcgatca tcgattgctc gccgttgaag tgcagcgtac
     8701 gcacttcgtt ccacgagttg cggtacagca ggtcaacgct acctaccagg caattttgat
     8761 tctcgccaaa gctgaagaca tccagcttac ggaaatcaaa atgcaccacc tgattgcgga
     8821 acgccgctgt cgggtcatat tccaggttaa cgataatcgc cagatggcgg atctcacacg
     8881 ggctgtagag cgccttcggt gtcggtgcag gtaagcgcag cgggaaatgg tgcgacacgt
     8941 cggcgaccat ctcctgcaac ttaggcaaat cgacaatgcc gttaccttta atatacaaac
     9001 gggtgcgcga ggtcagcaga ccgttaaacc atgcccacgc caccagttta ttcaggtaac
     9061 ggttatattc cagcggctga tggctgatga tcgactcaat atttggcgcg cggttataca
     9121 gataccaacc tgaacggtta gcccggcccg gcggcacata aataaaggtc agattcggtt
     9181 ccgagagatc gggtgaaatc tgcgggttta ccagcgttac tttacctggt aatgcttcaa
     9241 acgcggcata cagcttacgc gtcagcacgc cgatatcctg cggactggcg gagacgctaa
     9301 ggttattgcg acgcgcaaag cggatcagat tacggtagct ctgcatcatc gcgtcgagca
     9361 actcgttgtg cgcctcacgc acctgatcaa tcttccagtt agcacggtta tcgagcattg
     9421 ccagacgagc ttcgtcccaa ccccactcgc tcactaactg gctcaacact gcgcgacgcc
     9481 agcctacaca ggcgcgttca cggctgagtt tttcgcacac ttttaaatag aagcagcgac
     9541 gtactaaatc cagacgggtg aaatcttcaa tcgccgtcag gtattcagta acacgctcca
     9601 gcatcatgca gtatggatcg aggccaaacg atacaatctc gccgtcgtgc aaacgctgtt
     9661 tgatatcttt cgccagcaga cgtgggttcg ggtattccca ggaataggct tccagcagca
     9721 gtgttttcag caccgctttg tatggggaat cgatactctt gtagagctgc caaaggctgg
     9781 caccaaagta ctcttcagca gaaagcgagc ttaagccacc gagatccagc cattcatttg
     9841 gcgtcagcac gccctgcgcg taaagcgtca tcacatagtc gtcgtaatgc tcttcttcgt
     9901 cgcacggcac catattccac agaatacgct taccggcgag acgcacggcg gtacgataaa
     9961 attcgtcaag cagcagtata tgctgggtgg agccacaatc ttcgcccccc aggctgccgc
    10021 tttcattatg acgaaagcgg ttttcatcaa tcaggaagaa gctgacttcc acacccagcg
    10081 aggcggccca gctttccagc aggctgcatt tacgttgtag taactggcgc tcttcgctat
    10141 cgagccagga ttgatgacag acccagatat ccaggtcaga ggaacaactt tgccctacgg
    10201 acgaggtgct gcccatggtg tatacaccag taattggaag ctcacctttc ggtggatcct
    10261 gtactgacat tccacgatac agttcaagct cgttcaggta gtggcgttga gtttcatcag
    10321 gcgtgtaaag gcaaatgcct ttgggaacgt taccatcaag gtaacccggc attagcggat
    10381 ggtgatagtg caacaatgtc ggcagtagac tgtagacctg ttggaatgca ggccccatag
    10441 cagcaagcgc gcgatccaca cgcaattgat ttatggcatc cagtctctgt ttcagagtct
    10501 caatatagag gtacaagacg tatcgcctga tttgctaccc gtcatgactg tgattccgcc
    10561 aacatcaacg gtaacacgcg gcattcggga tatttcgtat gtcaaaggta accgttacca
    10621 cttttcgcgc ctggtttttt tagtttcacg acgaaaaaat ggtctaaaac gtgatcaatt
    10681 taacaccttg ctgattgacc gtaaagaaag atgcgctaca tacaagtgta gcaccgttta
    10741 ttctctgtaa attccttatt acgacagcgt gaaacgtctg tcaggatcca ctgccagacc
    10801 tcattttacg gtttgcgcag gcgtctacgt ttcaccacaa cactgacatc actctggcaa
    10861 ggatgttagg atggaccacg gatgataatg acggtaacaa gcatgttaga caatgtttta
    10921 agaattgcca cacgccaaag cccacttgca ctctggcagg cacactatgt caaagacaag
    10981 ttgatggcga gccatccggg cctggtcgtt gaactggtac cgatggtgac gcgcggcgat
    11041 gtgattcttg atacgccgct ggcgaaagta ggcggaaaag gcttatttgt aaaagagctg
    11101 gaagtcgcgc tcctcgaaaa tcgcgccgat atcgccgtac attcaatgaa agatgtgccg
    11161 gttgaattcc cgcaaggtct gggactggtc actatttgtg agcgtgaaga tcctcgcgat
    11221 gcctttgtgt ccaataacta tgacagtctg gatgcgttac cagcaggcag tatcgtcggg
    11281 acgtccagtt tacgtcgcca gtgccaactg gctgaacgcc gcccggatct gattatccgc
    11341 tccctgcgcg gcaacgtcgg cactcgcctg agcaaactag ataacggcga atacgatgcc
    11401 atcattcttg ccgtagccgg actaaaacgt ttaggtctgg agtcccgcat tcgcgccgcg
    11461 ttaccacccg agatttctct tccggcggta ggacaaggtg cagtcggtat tgaatgccgc
    11521 cttgatgata cgcgcactcg cgagctgctt gccgcgctga atcaccacga aactgcactg
    11581 cgcgttaccg cagaacgcgc catgaatacc cgtctcgaag gcggatgtca ggtgccaatt
    11641 ggtagctacg ccgagcttat tgatggcgaa atctggctgc gtgcgctggt cggcgcgccg
    11701 gacggttcgc agattattcg cggtgaacgc cgcggtgcgc cgcaaaatgc caaacaaatg
    11761 gggatttcgc tggcagaaga gctactgaat aacggcgcgc gcgagatcct cgctgaagtc
    11821 tataacggag acgccccggc atgagtatcc ttgtcacccg cccgtctccc gctggagaag
    11881 agttagtgag ccgtctgcgc acactggggc aggtggcctg gcattttccg ctgattgagt
    11941 tttctccggg tcaacaatta ccgcaacttg ctgatcaact ggcagcgctg ggggagagcg
    12001 atctgttgtt tgccctctcg caacacgcgg ttgcttttgc ccaatcacag ctacatcagc
    12061 aagatcgtaa atggccccga ctacctaatt atttcgccat tggacgcacc accgcactgg
    12121 cactacatac cgtaagcgga cagaagattc tctacccgca ggatcgggaa atcagcgaag
    12181 tcttgctaca attacctgaa ttacaaaata ttgcgggcaa acgtgcgctg atattacgtg
    12241 gcaatggcgg tcgtgagcta attggggata ccctgacggc gcgcggtgct gaggtcactt
    12301 tttgtgaatg ttatcaacga tgcgcaatcc attacgatgg tgcagaagaa gcgatgcgct
    12361 ggcaatcccg cgaggtgacg acggtcgttg ttaccagcgg tgaaatgttg cagcaactct
    12421 ggtcgctgat cccacaatgg tatcgtgagc actggttact acactgtcga ctattggtcg
    12481 tcagtgagcg tttggcgaaa ctcgcccggg aactgggctg gcaagacatt aaggtcgccg
    12541 ataacgctgc caacgatgcg cttttacggg cattacaata actctcataa caggaagcca
    12601 taatgacgga acaagaaaaa acctccgccg tggttgaaga gactagggag gccgtggaca
    12661 ccacgtcaca acctgtcgca acagaaaaaa agagtaagaa caataccgca ttgattctca
    12721 gcgcggcggc tatcgctatt gctctggcgg cgggcgtcgg tttgtatggc tggggtaaac
    12781 aacaggccgt caatcagacc gccaccagcg atgccctggc taaccaactg acggcattgc
    12841 aaaaagccca ggagagccaa aaagccgaac tggaaggcat tattaagcaa caggctacgc
    12901 agcttgaaca ggcgaatcgt cagcaagaaa cgctggcaaa acagctggat gaagtccagc
    12961 aaaaggtcgc caccatttcc ggcagcgatg ctaaaacctg gctgctggcc caggccgatt
    13021 ttctggtgaa actcgccgga cggaagctgt ggagcgatca ggacgtcacg accgctgcag
    13081 cgttgctgaa aagtgcagac gccagcctgg cggatatgaa tgacccgagt ctgattaccg
    13141 ttcgtcgggc aattaccgat gatatcgcca gcctttctgc agtatcgcag gtggattatg
    13201 acggcatcat ccttaagctt aatcagcttt caaatcaggt agataacctg cgtctggccg
    13261 ataatgacag cgatggttcg ccgatggatt cagacggtga agagctttcc agttccatca
    13321 gcgaatggcg tatcaatctg caaaaaagct ggcagaactt tatggacaac ttcattacga
    13381 ttcgccgtcg tgatgacacc gccgtaccgc tgttagcgcc aaatcaggat atctatctgc
    13441 gcgaaaatat tcgctctcgc ctgctggtcg cagcacaagc tgtaccgcgt catcaggaag
    13501 agacttatcg ccaggcgctg gagaacgtct ccacctgggt acgtgcttac tacgatactg
    13561 atgatgccac caccaaagcg ttcctcgacg aggtggacca gttaagccag caaaatatct
    13621 cgatggatct tccggaaacc ctgcaaagcc aggcgatgct ggaaaaactg atgcagaccc
    13681 gcgtgcgtaa cctgctggca caaccggcag cggggacaac ggaagctaaa cctgcacctg
    13741 cacctgcacc tgcacctgca cctgcacctg caccgcaagg tgatactccg gcagccgcgc
    13801 cgcaaggaga ataaccatgc taaaagtgtt attactcttt gttttgctga ttgcggggat
    13861 agtggttggc ccgatgattg ccggtcatca gggttacgtg ctgatccaga ctgacaacta
    13921 caatatcgaa accagcgtca cgggcctggc gattattttg attctggcga tggtagtgct
    13981 gtttgccatt gagtggctac tgcggcggat cttccgcaca ggcgcacaca cccgtgggtg
    14041 gtttgtcgga cgtaagcgtc gccgtgcccg taagcagacc gaacaggcgc tgctgaaact
    14101 ggcggaaggc gattatcagc aagttgaaaa gctgatggcg aaaaatgccg atcacgcgga
    14161 acaaccggtg gtgaactatc tactggctgc cgaagccgcg caacaacgtg gtgatgaagc
    14221 acgcgccaac caacatctgg aacgcgcagc agagctggcc ggcaatgaca ccattccggt
    14281 agaaatcacc cgtgtacgtc tgcaactggc ccgtaatgaa aaccatgctg cacgccacgg
    14341 cgtggataag ctgctggaag ttacgccacg ccatccggaa gtattacgtc tggcggaaca
    14401 ggcgtatatc cgcacaggtg catggagttc tctgctggat atcatcccat caatggcgaa
    14461 agcccatgtt ggtgatgaag aacatcgtgc aatgctggaa caacaggcat ggattggcct
    14521 gatggatcag gcgcgtgccg ataacggtag tgaaggtttg cgtaactggt ggaaaaacca
    14581 aagccggaaa acgcgtcatc aggtagcgtt gcaggtggca atggcggaac atcttattga
    14641 atgtgacgat catgatactg cccagcaaat tatcatcgat ggcctgaaac gccagtacga
    14701 cgatcgccta ctgctgccga ttcctcgact gaaaaccaac aatccggaac agctggaaaa
    14761 agtgctgcgc cagcagatca aaaacgtcgg cgatcgcccg ctgttgtgga gcacactggg
    14821 ccagtcactg atgaagcacg gagaatggca ggaagcatcg ctcgccttcc gcgcagcgct
    14881 gaaacaacgt ccggacgcct acgattacgc atggcttgcc gacgcgctgg acagactgca
    14941 caagccggaa gaagctgcag cgatgcgtcg cgacggtttg atgttaacgt tgcagaacaa
    15001 cccaccacag tagttccttc tcacccggag gcaagcacct ccgggacctt tctgatgcat
    15061 aaaaaaacgc ctgctcttat tacggagcag gcgttaaaac aggtctgtat gacaacagtg
    15121 ggtgcttcac tcaacgttgt gtccatggtg tctgatgagg cataagcgac atctgtcagt
    15181 ggacgataag caccgtaaac ggctctgcgt cattccggag tttatgaggc actaaggcga
    15241 acataagaga tggaatgagc atctacccgt ttattatgcc acagagaatc gggaaataac
    15301 atcccttaac acttgttatg agataattct gtaatcctct ttgcttcctg agtgaatatt
    15361 taacctgagc ttgatcctac acatacttat tatgaatgat aaaattcatt caattaataa
    15421 cacatatatt aattgccgtt aaaactaaaa aagcattaat aatcaacgca atataataaa
    15481 cccgccttac atatcaactg cgccagaggt aggattgaaa acgctctcct gattttccaa
    15541 ttcattttct ggataaataa ataatttatt tttgtcacta ttatttatgt aatcatcctg
    15601 tcagggagag ggatctcaat tatcaatgct taattacgtc atcattttga tgacatgaaa
    15661 ccggagcatt ttgcatggaa ttttcgtttt cacccaaact tcttgttgtt gctgtcgccg
    15721 ccgctcttcc tctcatggcc agcgcagcag ataccccgtc aactgccacc gcacgcaaag
    15781 gctttgccgg atacgatcac ccaaaccagt atccacagaa cgctgcaatt tattgaattt
    15841 gcacgatcat gtaggccgga taaggcgttt acgccgcatc cggcaatcaa ccgcaggcgg
    15901 ccacaaaatg gccgcttttt tacttattca ccaccagcaa atgtgcctgc ataatgtcac
    15961 tggccgggcg accgtgcgcc agcaaatcag ccattgcttt aagatatggc ggtagatggc
    16021 ggaaataacg ctgatacccg gcacacaaat aattcagccc cggtttgccg ctggcatcga
    16081 gcatgaagcg gtgtttcggg cagcctcccc agcacgcttt taacacgtta caactgcgac
    16141 actgcgccgg taattgctta aatttatctt caccaaacac ctgctgttgc ggggaatcga
    16201 tcatttctgc aattgtttgc tgatgcatat tccccagccg atattgcgga taaacatagt
    16261 gatcgcaggc gtaaacgtcg ccgttgtgct caacaatcac cgagcgccca caggttggct
    16321 gatgatggca aaccgcaccc ggcgcaccga caaaattggc aaacgcccat tcgatattca
    16381 tcacgaaaat cttgccgacg tcgcgtttga tccagtggtc gaatatcgcc accagaaatt
    16441 caccaaattc atcggggcgc accgaccatt ccgtcagttc cccctgaata tcaccaggcg
    16501 catgcaattt cagtccatcg cgggcagctg tttcatcagc cagacgctcg accaccggaa
    16561 taaactggat gaattcgact cccgcatcgc acaaaaaatc ataaacctgc aacggttgct
    16621 gcgcgctggt gcggttgacg cagaccagca cgttatagtc gacatgatgt ttttgcagca
    16681 gcgtcagggc acgcatcacc agcttatgcg ttggtctgcc acctttggtc acgcgatatt
    16741 gattgtggat ctcagccgga ccgtccagcg ataacccaac aagaaaatga ttttctgcca
    16801 gaaatgcaca ccatttatca tcgagcagca cgccgttagt ctggaagctg ttacttatct
    16861 tcctgccagc accgtatttc gcctgtaact ttacggcacg gcggtaaaaa tccagcccga
    16921 gtaacgttgg ttcgccgccc tgccaggtaa aagccacttc gttttgcggt tcgctggcag
    16981 cgatatagtg acggacatac gcttccagcg tgtcatcgtc catatgcgtg actggctttt
    17041 cgcggtaaag ggattgtttt tcgagataaa aacagtagtc acagttcaga ttacaatcgg
    17101 aaccactcgg tttcgccatc acatgaaaag cacgcgttgg aacctgttgc agcatggtca
    17161 ctccttgcct gaagtcatcc ttcaatacgt taagggcgaa atcataccgt ggggcaatga
    17221 gttattacgg gcgtttattg cgaagggagg gtgacgaaat gcacagaaaa caaaaaaccc
    17281 cgccgaagcg gggttcaaaa ttggtcggcg agagaggatt cgaacctccg acccactggt
    17341 cccaaaccag ttgcgctacc aagctgcgct actcgccgaa atactgcttt ttgaattttt
    17401 agttcaattc tttaaagtcg tggtgcgagg ggggggactt gaacccccac gtccgtaagg
    17461 acactaacac ctgaagctag cgcgtctacc aattccgcca ccttcgcatt gtcacaactt
    17521 ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac aatccagggc
    17581 tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta ctaccaccgc
    17641 agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag acctctgcct
    17701 ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg cggggggata
    17761 atacggactt cccacccctc tgtctagtcc ctttttaaat aaattgcgcg tttggttacg
    17821 gtttgtgcgt tttgctgctt attcctccag tttatgcgct ttcccgtggc gattaaggcc
    17881 aaaaacttta taaatcgccg tcaccgccag cataaagatg atgccaacaa acagcgacat
    17941 acgcgtatct tcattaaagt acatgccgat caaaacgcaa atcaggaatg ccattgttac
    18001 gtagttggcc cacgggaaca ggattgagcg gaacggatgg ctggcaatcg ccgctttatg
    18061 tgcacgccga aaacgcagct ggcttatcaa tatcacaaac catggcacca tccccggaag
    18121 cacgctggca ctgtagacgt agacaaacac acgctgcgga ttgggaatga tgtagttcag
    18181 gcatgagcca attagcagaa tagcaataga tactgccaca cccgcaactg gtacgccgtg
    18241 acgggaaact ttcgccatcg ccgccggtaa ctgacggttt ttcgccagtg cgtagagcat
    18301 acgtccgcaa ctgtacatgc cgctgttaca gccagagagc gcagccgtca gcaccacaaa
    18361 gttgataatg cccgccgctg cggtaatacc gattttggca aaagtcagta cgaacgggct
    18421 gccgttgctg ccaatttcat tccacgggaa gatggtgacg ataacgaaaa tcgcgcctac
    18481 gtagaaaatc aggatccgcc acagcacttt gcctacagca ctgcgcagcg tcacctgcgg
    18541 attcttcgct tcaccggcag taatgccaat cagctccacg ccctggtagg acgccaccac
    18601 aatacacagc gcggtcagga accctttcca gccacccgca aagaaaccgc catgctctgt
    18661 gagattgcta aaaccaatcg actgcccgcc attgccaaag ccaaagaaaa tcacgcccag
    18721 gccaatgaca atcatcacga taatcgtggt gactttgatc atcgcgaacc agaactcgat
    18781 ttcgccgtac aaccgcaccg ccgccagatt cgccaacgcc accagcgcca ccgcgatcaa
    18841 tgcaggtatc cactgcgcca tctccgggaa ccagaactgg acataaacgc caatggcggt
    18901 gatttctgag atccccaccg ccatccacat aaaccagtaa gaccaggcgg tgagataacc
    18961 aaaaaacggg ctcatataac gatgcgcata aacggcgaac gaaccggtaa caggttcaag
    19021 gaacaacatt tcgcccattg aacgcatgat gaaaaagacg aacagcccgg cgatgatata
    19081 ggccaacaat acggatggcc cggcccattt cagggtactg gcggccccca taaacaggcc
    19141 gacgccaata gtgcccccca gggcgatgag ttcgatatgt cgagcttcca gcccacgctg
    19201 tagctctggt ttgttatctg ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt
    19261 accggttatc gttatgggta catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg
    19321 gcaaataaag cattaaaaat ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga
    19381 gtgaccactc cgtcgcttta caaagagagg aaaatcagag gttgccggtg tagtgccagc
    19441 gtaaataacg cagcaaacga agctgacgct taatgcggct cggctgcgaa agcaggcggt
    19501 agagccactc cagtcccagc gtttgccaga ttttcggtgc gcgttttacg tgaccggtga
    19561 aaacatcgta agtcccgcca acgcccatat acagcgcatc tggatgcacc agacggcagt
    19621 cgcgcatgaa gatctcctgc tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc
    19681 tggcatgaat gcgttcaaac agcgcctgac gctgctcggg tttaaaataa ccatcctgac
    19741 tgccaacgat attcacattc cattggttgc gcagtttagc ttcagtttgc gccagcactt
    19801 caggtttacc gcccacaaga aataccggcg tcccttcttt gcctgcgcgc gccatcagct
    19861 cttcccagag atcggcaccg gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta
    19921 ctgaacgtac aacgctgatg ccatccgcat atttaaattc ggcagcgtta attaactccc
    19981 tgacctcggc gttatcttca atggtcagca ttttttcagc attaatggca accagcgttc
    20041 cctgcttaag ctgcccgtca gcaaacagat aatcgagggc gtgctgcata tcacgccaac
    20101 caatcaactg taagccacgc agcgtatagg tcggtgccgt ggtgttgtta ttcattgtta
    20161 tccttcaacc tgcgtccgga gcgatgattt tgtacgttta tgaatgagtc cggcgctttc
    20221 aaaaagccag tacagcagtt ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa
    20281 gactacgcgt gagacaaacg aatccagccc ttcacgcgcc agcacgatca tattgaagat
    20341 cgccccaaag cagaaactgt gcaatatcgc agccttatag cgattaggct cgcggttgcc
    20401 cagctcatac agccagtcga accatttgat gatcagacca accacgatcg ccccgagcgg
    20461 gatgaacaac gcgccgccca tcaccaccag tgagcctata agcgtaggcg agatcgccag
    20521 tccggagtgg ttattcagca cttcccaggt aaagtagttg gctgagttca gcaccatact
    20581 cgggcgaccc ggccacagcc aggaagggat aaagacatag aaatcgcgga caattggagc
    20641 caggccctgg aagtcgatgt tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg
    20701 ggagaaggtg tcgcgagtga gatagagaaa cgtatagaac gcttcatcgc cgctcacatt
    20761 cattccatag cgttttagcg ccagccagaa catgccaaca atccccagca cgcccgccgc
    20821 cgccagcatc cacaacgaaa tccagccgcg aataatgcca ataaacagga agatagcgaa
    20881 tgcgatgatg atattggcgc gagtgccgcc gacaatcata taagtcagca agccaaaggc
    20941 gaccgtgctg acgaggaaaa acagccacgc tttgctgtcc tggcgcagaa agtagaccac
    21001 cagcatcgcc gggatgaaaa agtaaaagaa gcgttttaac gccacgccgg agacttcact
    21061 ggagaagatc tgactgtagg agttaagccg gaacagcaaa aagccgttgt gcatgaagaa
    21121 gatgccgacg cttaccagcg cgatacccat caggatcacc cacgtaagat tggtctccac
    21181 gcggttcatg gtaaacagcg gacggcgcgg tacatcagca acgcgtttgc gtaggcgggt
    21241 tttgtaggtg acatagtaaa ccgcgtagaa gcagcccgca gaaagcaacg cctgcaacaa
    21301 gatttctggc ggcgcgacac caacatcaaa gcgaaatacc agcacgctgg tcagcgggaa
    21361 gccgaagaaa aaggtgagca aaaacagcaa tgaaaagaag acattgaagt taaagcgcac
    21421 acggcgaaac tcaaaccagg tcagcgtggc aataaacagc gtgcagagca gccagaccac
    21481 aaacaggcca ctgaattgca gcagactcat gcgacctccc cggcggcaat agccaacgcc
    21541 cgctgccagc cttgtagata gttagggcta aagaaggcaa tggtgttttt atccaccgac
    21601 gccaactggc gctgcgcttc acgcacaata tcctcgttga gatcgtcggt agtaaacagc
    21661 accggcaaat gctgttccgt catatcctgc cagaacggat tttcccggtt aagcacacaa
    21721 ggaatgcccg cctgaatcag taagcaaagc gtaccaatgc cctgctggcg ggcaaaaata
    21781 aagtaaccaa gatcgcactg acgaagtagc gccagatagg cgtcaaattc cagtttttcg
    21841 ctcaaaattt gcagattttc ttcgctgaat aactccagcc ccgcctgacg aacttcctca
    21901 atgtacgctt cgttattagg cggatatccc atcggcacca ccacttttac cgtatcgcca
    21961 aattgctgat gaacggcgcg caaggcagca atatggtcat tgctgcggtc accggagttc
    22021 cccaccagaa tggtcatttt cccttcacgt tgccgatcat tcgccatcgt attgagagaa
    22081 gggtccatcc gcgtcgggaa gtacagcagt tcgccccgca cctttggatg cgttttggca
    22141 aaaaagctca aatcaccgcg ggtggcaaat acacagccga ctcgcttttg cgccaggcga
    22201 cgtagcgggt aaaaaagctt atatctcaag ccactggaaa gctcatacag gtctgccccc
    22261 cagatatgcc agaaaaactg gctgggctta atgccaccac tcagcagagc cagccacagt
    22321 gtgggattga actgaccgtg gaaaaaaaat cgctgctgac ggttagcttt tgctttcgcg
    22381 atgaccgctt ccgccagcga ttttttccca gggaaaaatt gcacagaaag cgccggacag
    22441 ctatcactta agccgtcgtc cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc
    22501 gcggccagcg cgtcattgaa aaaccgcaaa acggttcggt tatggtgagg gatatccgat
    22561 cccagtacgt gaatcagtac agtcatgccc gcctacgcca gagtaaaaac acgccacaac
    22621 aaagagaaaa atagacgata taagttgcca tatatgcctg cgccgcgccc agtgcaccat
    22681 gcgcagggat tagccagtgg gcaaatacca tcaataaagt gaactggctg acttccgcca
    22741 gaatataaaa ccgcagtgac gctttggcga tcaccagata accaaagaca taagcgccca
    22801 cttttaacac atcacccact aactgccagg caaagagatc gcgcatagcg gtaaatttat
    22861 tcgacaacag caaccagata gcaaaatcac gcagcaacca gacggtaaaa ctcgccgccg
    22921 ccactgccgg taagacgaat ttcagcgatt taaccacttc ccgggtgata tcgcgctttt
    22981 ccgttagccg cgacaacgtg ggcagcaaat atacgctgaa cgatgccgta ataaattgca
    23041 ggtaggcatc ggaaatactg ctcacccctt gccagatccc cacttcatcc cagctatact
    23101 gcgccgccag cagtttacgc atcatgatgt aagcaacagg caaggtcacc gacgtaatca
    23161 acgccatgag cgtaaatttg ctcaactgcc ctgccagacc gttatcccag ctgggtttca
    23221 gatagcttaa cgggatgaca ccacgtttga tcaacatgat ggcggcagga atcaccacca
    23281 gcgcgggaat cagcgccaga cccagcaacg ccccttcata accgcccaaa cggtaactga
    23341 cgtagtacgc gagaacgcca atcaagctgc cgacaatcag cgataacgca ttacctgcgg
    23401 catcgcgaaa gcctttcatc agcgccagta acaggttgcc ccaggcgatc cccatttgca
    23461 ccagcgccac taaacgcacc agcccctgat agtcggtatt accaaacagt ccctggctga
    23521 ttggcgcagc tgccagcaca aaaaccagcg ccatcagcgt agagaaacca agtaccatcg
    23581 ctgatgaagt gccgaccacg cggcgcagct gttgcggatt atcatggtac tgggcaacgt
    23641 atttggttac accgttaaag atgccagctc cggcaagcac gccgagcacg gtaatcaact
    23701 ggcggaaatt tgccgccagc ccaagccccg ccggaccaaa tgacacagcc agcaacttac
    23761 cgaccagtaa cccggcacca atcttgacca gtgtactggc cgccgtccac aaggacgctt
    23821 ttgccaacga catatcagga gaagtagttc aacaaagtcg caattaccgt acgctgattg
    23881 acgggcgaca ggttgtagaa cagcggcagg cgcagcaggc gctcgctctc tttggtggtg
    23941 tagcgatctt caccgtggaa ctcaccaaag cgttcccccg cagggcaacc gtgcagcgga
    24001 atgtaatgga acaccgccat gatttccgct tctttcagaa agttaatcaa cgcgctccgg
    24061 tcatcaatat cccgcagttt aatgtagaac atatgcgcgt tctgcacgca gccatcggga
    24121 atcgacggca gctcgatacg cccggctttc gccagaggcg ctaacgcatc gtagtagttt
    24181 tgccacagcg ccagacgttg ctggttgata cgatccgctg cttccagttg cgcccacagg
    24241 tacgcagctt gcagatcgga catcaaatag ctggagccaa tatcgcgcca ggtatattta
    24301 tcgacctgac cacggaagaa ctggctgcgg ttagtgccct tttcacggat gatctcggct
    24361 cgttcgatta acgctttatc gttaatcagc gtcgcgccgc cttcaccacc cgccgtatag
    24421 tttttggttt catggaagct aaagcagcca atatgaccaa tggttcccag tgcacgccct
    24481 ttgtaagtgg acatcacgcc ctgagcggca tcttctacca caaacagatt atgctttttc
    24541 gccaacgcca taatggtgtc catttcgcag gccacacccg cgtaatggac cggcacgata
    24601 acgcgcgttt tgtcggtgat cgccgcttca atcagcgttt cgtcgatgtt catggtgtcc
    24661 gggcgaacat ccacaaaaac gatttttgcg ccacgcagca caaaggcatt ggcggtggag
    24721 acaaaggtgt agctcggcat gatcacttca tcgccaggct ggatatcgag cagcagcgcc
    24781 gccatctcca gcgaagcggt gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt
    24841 tgctccagcc actgctggca gcgacgggta aaaccgccat cgccacacag tttgccgcta
    24901 cccattgccg actgcatata gtcgagttcg gttcccacca ccggcggtgc gttaaatgga
    24961 atcatgtgat cacctgtata accagtacgc ggtgctttct acattcgcac cactttgtat
    25021 gtatcgttta agcgcggcgg tgttgcccat ttgggtcgcc acccgcaaag ttgttttacc
    25081 gcgagcatac gcccagttta gcgccgtttg catcagctca gcacctgcac cgcgtccagc
    25141 cagcaggcca attcgcgcat ctgtcgcatt gagttcccgt aaagagacat aaccgcgaat
    25201 accgccggac gccgcacgta aaatcagaca ttgatgatca aaggtgccgc gcacggcatt
    25261 ttcaatccac tgtgcataaa agcgtccgct ggcgtcaggc gcataccacg gcgcacgaaa
    25321 acggctttgc gcaaatgcgg cgctggctaa ctgacgtaat gcgggaatat cggtctcttg
    25381 tgccactaca gcaccactat cactgacatt gttcacgggt agtgccaaat caacttcacc
    25441 ttctaccagg gagaatccca gctgttgcag ggcatccagt tcacccgtat ttgatgccgc
    25501 aattttggcc tgcacccgtg accacggcgc taacacgtct ggcgtcagga gctgtgcttc
    25561 agacgtaatg cgcacgatgg cgctgttaac accaaagaag gcgttttccc aggttagtgg
    25621 ctcaatactg gcgcggacgg gcacgaagta actccagcag atattggccg tagccagttt
    25681 tcgctaatga actggcagca cgcttcacac cctcgtcatc gagccagccg ttacgccagg
    25741 cgatctcttc caggcaggca atcttaaagc cctggcgttt ttctactgtc tgcacaaagg
    25801 tactggcttc aatcaggctg tcgtgagtgc cagtgtccag ccaggcaaat ccgcgcccga
    25861 gcagttcaac ggtcaggttg cccgcctcga ggtacatctg gttgatggag gtaatctcca
    25921 gttcaccacg ctccgacggc ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga
    25981 aataaagccc ggtcaccgcc cagtttgact tcggctgttt tggcttttct tccagcgaga
    26041 tagcgcggaa attgtcgtca aactccacca cgccaaagcg ttccgggtcc atcacctgat
    26101 agccaaatac cgtcgcccct tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc
    26161 cctgaccgaa gaagatgtta tcgcccagca ccagacaaga aggttcaccg ttgaggaagg
    26221 tttcaccgat gataaaggcc tgcgccagac cgtccgggct gggctgttcg gcgtattcca
    26281 gctggatacc gaactcacta ccatcgccca gcaggcgctg gaaataacct ttatcttccg
    26341 gcgtagtgat gatgagaatt tcgcggatat cggccaacat cagcaccgac agcggatagt
    26401 aaatcattgg cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc gtaatcggat
    26461 gcaatcgggt gccggaaccg cccgccagga taataccttt catgtttgcc tccgctgaaa
    26521 attagccttt cagacctaaa cgctcgccct gatagctgcc gtcctgcacc tgcttccacc
    26581 agctttcatt agccagatac cactgcaccg ttttacgcat tccactttca aaggtttcct
    26641 gtggcagcca gccaagttca cgggcaattt tcgaagcatc aatggcataa cgcagatcat
    26701 gccccggacg gtcagcgaca aaggtgatca agtcacgata gtgcaccacg ccgtgcggct
    26761 tgttcggagc cagttcttcc agcagctcgc aaatggtttc cacaacatcg agattcttac
    26821 gctcgttgtg accaccaata ttataggttt caccgacttt cccggtggtc gccacgcaat
    26881 acagcgcgcg ggcgtgatct tccacataca gccagtcacg gatttgctgc ccgttgccat
    26941 ataccggcag cggtttaccc gccagcgcgt tgaggatcat cagcgggatc agtttttccg
    27001 gaaagtggta aggaccgtag ttattcgagc agttggtgat aagcgtcggc agaccgtagg
    27061 tccgcagcca ggcgcgcacc agatggtcgc tgctggcttt tgacgcggaa taagggctgc
    27121 tcggcgcata cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg tcaccatata
    27181 cttcgtcggt ggagatatga tgaaaacgga acgctgattt tttatcttcc gtcagcgtat
    27241 tccagtaagc ccgcgccgct tcaagcaatg tataagtccc gacaatgttg gtttcaataa
    27301 acgctgccgg gccgtcaata gaacggtcaa catggctttc ggctgccaga tgcatgacac
    27361 agtctggctg atgctcagtg aatacgcgtg ccagttctgc ccgatcgcag atatcaactt
    27421 tctcaaaggc aaagcgctcg ctttgcgcga ccggtgccag cgacatcagg tttccggcgt
    27481 aggtcagctt atcgaccact accaccgcgt cgctcgtttc gttgatgata taacgcacca
    27541 gcgccgagcc aataaacccg gcaccacctg ttatcagaat ttttctcatc agcgccagac
    27601 tcctttggca tcgacgacat actgctgatg gacattgtcg ccattgataa ctttaaactg
    27661 actatgatcg accagcatca ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc
    27721 cagagtacaa agcccggtca gttttttcgg caactggtgg atgttaggct caacaaccag
    27781 agtttcgccg ctatgccact gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg
    27841 caggtcatca atattcggtt taaacgccag accaaagcag gcgattttca gttcactggc
    27901 gcgtttatcg gtagccgcca ggcaatcagc caccgccgct ttcacctgat cgataaccca
    27961 gaacggtttg tgatcgttca cttcgcgcgc ggtacggata agccgcgcct gctgggggtt
    28021 ctgtgccacg ataaaccacg gatcaacagc aatgcagtga ccgcccacgc cagggccagg
    28081 ctgaagaata ttaacgcgag ggtgacgatt cgccaggcga atcagttccc agacgttaat
    28141 cccctgatcg gcacaaatca gcgacaattc attagcaaaa gcgatattca catcgcggaa
    28201 gctgttttcg gtgagcttac acatttccgc cgtccgcgag ttagtgacga cacactcacc
    28261 ttcgaggaaa attttgtaca gttcgctggc gcgggtcgaa caaaccggcg tcataccacc
    28321 aatcacgcga tcgtttttaa tcagctcgac cataacctgg cccggtaaca cgcgttccgg
    28381 gcagtaagca atgttgacgt ccgcctgctc gcccacctgc tgtgggaaag tgagatccgg
    28441 acgcatctct gctaaccatt ctgccatctt ctcggttgac cccaccggcg aggtggattc
    28501 aaggatcacc agcgcgcctt ttttcagcac tggcgcaatg gagcgagcag ccgattcaac
    28561 gtaggtcata tctggctcat gatcgccttt aaacggcgtg ggtacagcaa tcagccaggc
    28621 atccgcttca actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg cagtttttac
    28681 tacgctcgcc aaatcaggct cgacgatatg gatttcgcca cgattgatgg tatcaaccgc
    28741 gtgttggttg atatcgacac caatcacctg tttttgccgt gaggcaaacg ctgctgctgt
    28801 aggcagcccg atgtaaccca gtccgataac agaaatggtc gcaaaactca tagtgatatc
    28861 cgattatttt ttaacgcttc cagaatgcga gagcatgcct gaccatcacc atacgggtta
    28921 tgggcgcggc tcatagtttg atattcgttt tcgtctttta aaagacgcgt cacttcctcg
    28981 acaattcgct gcttatccgt gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga
    29041 cgctcagtgg tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc ttcttcctga
    29101 atgccgcctg agtcggtcaa aatcagccag gcgtggttca tcagccagac aaacggtaaa
    29161 tactcctggg gatcgatcag aatgacattt ttcacatgcc ccagaatgcg attgaccggc
    29221 tctctgacgt tcgggttgag atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg
    29281 gcaatgtctg ccaacgcgtg gcagatttct tcaaagccac gaccgaaact ttcacgcctg
    29341 tgaccggtca ccagaatcat ctttttatcg gggtcgataa acgggtaatt tgccgccagt
    29401 tctgaacgca gcgtatcgct gctcatcacc tggtcacgca cccataacag tgcatcaata
    29461 actgtattac cagtaatgaa gattcggcta tccgcaacgt tttcacgcag caagttttgc
    29521 cgggaagttt cggttggaga gaagtgatac atcgccagat gcccggtcaa tgtacggtta
    29581 gcctcttccg gccacggcga ataaagatcg cccgtgcgca gaccagcctc aacgtgacca
    29641 acaggaatac gctgataaaa cgccgccagg ctggttgcca gcgtcgtcgt tgtgtcgccg
    29701 tgaaccagca cgacgtctgg tttgaactcg gcaagaatag gttttagccc ttccagaatc
    29761 cgacaggtta tctctgtcag gccctgtcct ggctgcatta tgttgagatc gtagtcaggt
    29821 acaatggaaa agagtttcag cacctgatcg agcatctccc gatgctgcgc agtgacgcaa
    29881 actttagcct caaaaaaagg atcttttgcc aacgcatgca ccaacggcgc catcttgatg
    29941 gcttccgggc gcgtaccaaa tacagtcagt actttcacat cgattctctt cgaataagcg
    30001 gcgagcgcct ttgcgctcac cgcagcagtg ttgctatttc gagcaacggc gggttaatgc
    30061 gacaccagcc ccgatcagcc ccccgacaat gccccacata atcatcagga aggcacgacg
    30121 tgggctatcg cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg
    30181 cggatccagg gttggaccaa cattcagggt gtttaacatg gcccgattct gatcatagtc
    30241 gagatcaaag gccggaccga cggcctgtaa attttccagt cgagcctgga gcattggacg
    30301 cccaagcagg aacatttctg aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg
    30361 cgaaatatta tgctgctcag caattttcag cgcctgttca atactgttca tccggcggtc
    30421 gtagatggct ttcgccacct cttcctgacg cttcacctgt gctttcatct ggatggtacg
    30481 cgccgcccat gcgcctttca gctcatcatt cagatggctg gctgcacgct ggctggcaaa
    30541 agcaacatac tgacgtaaca ggttattagc gtcaggcgcg gtttcagcaa taagcttcac
    30601 gctgtcattg accgcgcggg taaagtctcc ggggataaac tggatgttgt taatcatttc
    30661 atccagcaac gccgcatcgg ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt
    30721 ttgcagccag aactctctgc gggtatccca cgaggccagt tgcataacaa actctttata
    30781 ggcttcgtcc atgaccgatg gttggtcggc agaagccatg tttgaacgga catccaggtt
    30841 acgcaaaaat tgctgctgcg agtaatatcc ccccagcata ttcaccgttg gacgatcggt
    30901 aatcgccgtc gagctccact cctgacgagc aaaaaaagta tacgccagcg cgattaacgc
    30961 aaatgccagc cccatgccaa taatccatag cttcccagcc cacaaggtac gaaacaaccc
    31021 acgaatatcc agttcatttt cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat
    31081 cacatcctca tttatttggt taaattgggg ctgccaccac gatttctacg cagtctgcgt
    31141 tttacgcgct taataaagcg agcaactttc caggcacgct taatgcaata tccatagagg
    31201 aagaatgcta gcaaaaagag caccagcatg acccactccg ggacaaaatg agaatattct
    31261 gccagcacgc caatggaagc gagcagtgcc gcagcaaggg taatcagcac aaacgcctga
    31321 cgggaagtaa acccggcacg catgatcaaa tggtgaatat gctgacggtc aggagagaat
    31381 gggctcatgc ctttacgcag gcgacggtac ataatcgcca ccatatccat tagcggaatg
    31441 gcgattatcc acaaagcggt aaccgggctg atgggatggg ttttgccctg ggtcgtttcg
    31501 agcaggatcc agataacggt aaaaccaatc agcgtactgc ccgcatcacc cataaagact
    31561 ttgtagcggc gacccaggat accaaggtta agcatgatgt atggcaggat ggcggcgatc
    31621 atcgcaaagc accagattgc gaggctggtt tgcccgtcga accacaaaat cataccgatt
    31681 gctgcaaacg agacgcagga caacccgccc agcaagccat caatgccatc aaccatgttg
    31741 aacgcattaa tggccgccca gacggcaaat agcgtcagga agtaaccaaa cggtccgagc
    31801 accatctccc aggagccaaa gatataaccc aggctactga gataaagttt gccgaacacc
    31861 atcataacaa tgccaacagc ggcctgtatg gtggcacgga tttttacgct gatatcaaaa
    31921 cggtcatcca gcgcgccaat gaaaacaagc acaccggcac aagcgagata gagagatgca
    31981 tgcggaatat agtaatcgac aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc
    32041 cccccaacga gaggtatcaa tccctggtga cgtttgcgga agtttggttt atccactaaa
    32101 ccgacttttt ttgccacctt acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata
    32161 ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc atgctctcag agaaaattat
    32221 tagcagaagt ataaccacga agacctttat tcagaaggga aagtccgttc cgaacccacc
    32281 agtcctgtgc aaaaattaac catttgatat gcaaattgca taacacgcca gcaaattcca
    32341 ggaattttcc taacatcgcc agcgcggcat taagattaca gatataaaag caaaacgcca
    32401 cgtaaacacg tggcgttttt ggcataagac aaatttatga gcgtttcatc atttcgaaga
    32461 aatcgtcatt ggtcttggtc atggccagtt tattaatgag gaattccatt gcatcgattt
    32521 cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt ctgcagttct tcctgagtag
    32581 tgagcagttc ttctttacgg gtaccggaac ggttgtagtc gatagccggg aagacgcgtt
    32641 tttcagcgat cttacgagag aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt
    32701 agataacttc gtccatttta gaaccggtat cgataagcgc cgtcgcgata atagtcaggc
    32761 tgccgccctc ttccacgtta cgcgccgcac caaagaagcg tttcggacga tgcagggcgt
    32821 tggcatccac accaccggtc aacactttac ctgacgccgg aacaacggtg ttgtaagcgc
    32881 gcgccagacg agtgatggag tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc
    32941 gtttggcctt ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa
    33001 aggtagaagc aacaacttca ccttttacca gacgctgcat ctcggttact tcttccggac
    33061 gttcgtcgat cagcagaacc atcagcacac aatccgggtg gttgtaagca atgctctggg
    33121 caatgttctg cagcagcatg gttttaccgg ctttcggcgg tgccacaatc agaccacgct
    33181 gaccacgacc gataggtgat gccagatcca gtacgcgagc agttaaatct tcagtagaac
    33241 cgttaccacg ttccatacgc agacgagaat ttgcgtgcag cggggttaag ttctcaaaga
    33301 ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg
    33361 caaaatagcg ttcaccttct ttcggcgggc gaatcttacc agagatggta tcaccagtgc
    33421 ggaggttgaa acggcggatt tggctagggg aaacgtagat gtcatcagga ccggcgaggt
    33481 aggagctgtc tgcggaacgg aggaaaccaa atccatcctg caatatctcc agtacgccat
    33541 caccaaagat atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct
    33601 gcttacgcat gcgagccagg ttttccagcc ccatattttc gccgagagtg atcagctcag
    33661 aaaccggcgt attctttaat tcggtaagat tcatagtggt gtgagttctt aaacttgggg
    33721 taaatctcga acttaatgtt gtgaatggta tggcaggatc atccatgcct gtttacggtc
    33781 atcgactcat gtcttttcgc tgcctggtca caggagagta cgcagaactg aaacgacaag
    33841 acggattgag tgacgagcca gaaatctgtt cacttcgcat ttaagataaa acgggaagcg
    33901 ttggaaatta caagattcaa acttaataag gtatgtttaa tacgaagtca acactaagtt
    33961 agcatgactc acgccgggcg tccagttttt agcgacgggg cacccgaaca tgaaattccc
    34021 ttacgccagg ttagcgtcga ggaactcttt caactgacct ttagacagtg cgcccacttt
    34081 ggttgccgcc acttcaccgt ttttgaacag cagcagagtc gggataccac ggatgccata
    34141 tttcggcgca gtgccagggt tttgatcgat gttcagtttt gcaacggtca gtttgccctg
    34201 atattcgtca gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc
    34261 tgcccagaaa tcgacgagga tcgccccgtc cgctttgagt acatccgtgt caaaactgtc
    34321 gtcagtcagg tgaataattt tatcgctcat atataactcc acaggaataa gcctggcgtg
    34381 ttggtttcgt tgttggtgta acattaacca actaaaggtt gactttattt caccggatac
    34441 gctttcgtaa agcaatagta agctgatatt ctaccacact atgagcaaaa cacatttaac
    34501 agaacagaag ttttccgact tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa
    34561 agggtttcat aactgtacgc ccattcaggc actggccctt ccgctgacgc tggcgggtcg
    34621 tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac
    34681 gtttcattat cttctctctc atcctgcgat tgccgatcgc aaggtgaatc aaccgcgtgc
    34741 cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc catgccgacg cagaaccgct
    34801 ggcggaagct actggcctga agctgggtct ggcttacggt ggtgatggct acgacaaaca
    34861 gctgaaagtg ctggaaagcg gcgttgacat tctgattggc accacgggtc gtttaattga
    34921 ctacgccaag cagaaccaca ttaacctcgg tgccattcag gtagtggttc tggatgaagc
    34981 cgatcgcatg tacgatctgg gctttattaa agatatccgc tggctgttcc gccgtatgcc
    35041 gcctgcaaac cagcgcctca acatgctgtt ctccgccacg ctttcgtacc gggtacgtga
    35101 actggcgttc gagcagatga acaatgccga atatattgaa gtggaaccgg aacagaaaac
    35161 gggccaccgt attaaagaag agcttttcta cccttctaac gaagaaaaaa tgcgtttgct
    35221 gcaaacgctg atcgaagaag agtggccaga cagagcgatt attttcgcca ataccaaaca
    35281 ccgttgtgaa gagatctggg gccacctggc ggcagatggt catcgtgtcg gtttgttgac
    35341 aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc gtggcgatct
    35401 ggatattctg gttgccaccg acgttgccgc gcgtggtttg catattccgg cagtgacgca
    35461 cgtctttaac tacgatttac ccgatgactg tgaagattac gttcaccgta ttggtcgtac
    35521 aggtcgcgca ggcgcaagcg gtcactctat cagcctggcg tgtgaagagt atgcattgaa
    35581 tttgcctgct attgaaacct atattggtca ctcaattccg gtaagcaaat acaatccgga
    35641 cgcattgatg accgatctgc caaaaccgct gcgcctcacg cgcccacgta caggcaatgg
    35701 tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac
    35761 tcaaaatcat tcaaactgta tcaagatggc aagagaatga atcccgatga gcatactgta
    35821 gtaagtgatt caggtgaaag aatgcagcca acacagagac agattgaagg atgaagagta
    35881 tgggttccac ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc
    35941 tggttgtgcg cgaggtggct ggaagcatcc agacgctgac gcgaataaaa cgcaaagtgc
    36001 gtctggctgc tggcctgaat agcgaaaatg ccctgtctaa tgaagcaatg gagcgcggtt
    36061 ggcaatgtct gcgcctgttt gctgaacgtc tgcaagatat ccctccctcg caaattcgcg
    36121 ttgtcgctac ggcgacatta cgtcttgccg tcaatgcggg tgattttatt gccaatgcgc
    36181 aggaaatcct cggttgtccg gtacaggtga tcagcggtga agaggaagca cgtctgattt
    36241 atcagggcgt tgctcacaca actggcggtg ccgatcagcg cctggtggtg gatataggcg
    36301 gtgccagtac tgaactggta accggcacgg gtgcacaaac cacctcgttg ttcagcctgt
    36361 cgatgggctg cgtcacctgg ctggaacgct attttgccga tcgtaatctg gggcaggaaa
    36421 attttgatgc tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc gatgaattac
    36481 ggtatcacgg ctggaaagtg tgcgttggtg cttccggcac cgtgcaggcg ttacaggaaa
    36541 tcatgatggc acaggggatg gatgaacgca ttaccctgga aaagttgcag caattgaaac
    36601 agcgagccat tcattgcggt cggctggagg aactggagat tgacgggctg acgctggaac
    36661 gtgcgttagt gttcccgagt ggtctggcga tcctgatcgc catttttacc gaactgaata
    36721 ttcagtgtat gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc
    36781 atctggccgt cgagcaggat attcgcagcc gtacgctgcg taatattcag cgccgcttta
    36841 tgatcgatat tgatcaggcg cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg
    36901 tggaaaagga atggcatctt gaagcaataa gccgcgattt gctcatcagc gcctgtcagc
    36961 ttcatgaaat cggcctgagc gttgacttta aacaagcgcc gcaacacgct gcttatctgg
    37021 tgcgtaatct ggatcttccc ggttttaccc ccgcacagaa aaaattgctg gcgacgctac
    37081 tgctcaacca gactaatccg gtcgatctct catcgctgca tcagcaaaat gccgtaccgc
    37141 cgcgcgtcgc agaacaactc tgccgtttac tccgcctggc catcattttt gccagccgtc
    37201 gccgtgacga tctcgtgcca gagatgacat tacaggctaa ccatgaactg ttgaccttga
    37261 cgcttccgca aggttggcta acccaacatc cgctgggtaa agagattatt gctcaggaaa
    37321 gccagtggca gagctatgtc cactggccgc tggaagtgca ttaatgagta agtgccggat
    37381 gcgatgctga cgcatctttt ccggccttga ttatttccct cgttttgccg ccatcatcgc
    37441 tttcagattc gccagatggc tttgcccttt ctgcatccgt tcttcggcgc tgaccacttt
    37501 gcgctcctgt tcccaaatca gatcatcctg cggcagctcc agcaaaaagc ggctcggctc
    37561 cgggcgcacc agctcgccgt actgacggcg ttctttacac agcgtaaagg tcaattcctt
    37621 ctgggcgcgg gtaatgccga cataggccag ccgccgctcc tcatcgatat tatcttcatc
    37681 gatgctgctc tggtgcggca aaaacccttc ttccataccg accatgtaga cataaggaaa
    37741 ctccagccct tttgacgcgt gcagagtcat cagttgcacc tgatccagct cttcttcact
    37801 ctcaccacgc tccatcatgt cgcgcaaagt aaagcgcgtc accacctggg tgagcgtcat
    37861 cggctcatcc agttcgctgc cttccagcat ctccgtcatc cagctaaaca gttggttgac
    37921 gttcttcatg cgcatttcgg cggctttcgg gctgggcgat gtttcgtaca gccaggattc
    37981 ataatccatg ccgtggatca gatcgcgcac cgcggcaatc ggctcccgct ccgccagacg
    38041 ctggatttct gccaaccagt gagtaaagcg ggtcaatgct tcataaccac gtccgctaag
    38101 cgtctgactc aggcccatat caaagctggc ggtaaacatg cttttattgc gcgtcatcgc
    38161 ccactcaccc agctttttca gcgtcgccgg gccaatctct ctcttcggcg tgttaacgat
    38221 acgcagaaat gcgctgtcat cgtccgggtt agtcagcacg cgcagataag ccagcaagtc
    38281 cttgatttca ggacgagaga aaaacgacgt accaccagaa attttgtacg ggatgcggtt
    38341 ttgcatcagg aatttttcaa acactcgcga ctgatggtta ccgcgataaa gaatagcgta
    38401 atctttgtac tgcgttttat tgacgaagtg atgcgcaatc agttcaccag tgacgcgctc
    38461 ggcttcatgt tcttcgttat tcgcgcttaa aactttaagc tctgtgccgt aacccagttc
    38521 ggagaacaga cgcttctcaa agacgtgcgg gttattggcg atcaggatgt tcgccgcttt
    38581 caaaatacgt ccggaagagc ggtagttctg ctcaagctta atcaccttca gcgccggaaa
    38641 atcctgactc agcagcacca ggttttgtgg acgtgcgccg cgccaggagt agatcgactg
    38701 gtcgtcatcg cccaccacgg taaagcgcgc gcggctgccc accagcagtt tcaccagttc
    38761 atactggctg gtgttggtgt cctgatactc atccaccagc agatagcgaa ttttgttctg
    38821 ccagcgctcg cggacttctt cattgcgttg cagcagcaac gtcggcaata aaatcagatc
    38881 atcgaagtcg agaacattgc acgctttcag gtgtgcatca tacagcccat aacaatgggc
    38941 aaaaatacgg tcccgctcgc caatcgcact ggctgccgcc tgggacggtg ttttgagatc
    39001 gttcttccag ttagagatgg tcgaaatcaa ctgttgcagg agaactttgt catcttcaat
    39061 cagcccttcg gtcaactctt tgagcaacgc cagctgatcg gtatcgtcaa acagcgaaaa
    39121 gttcgccttc atccccagcg ccgcatactc acgcttgatg atatccagcc ccagcgtgtg
    39181 gaaggttgag atcatcagcc cacgcgcctc tttgcgaccc agcgtctgtc cgacacgctc
    39241 tttcatctcg cgcgccgcct tattggtaaa ggtcaccgcc gcgatatgtc gcgcctgata
    39301 accgcaaccg cggatcagat gggcgatttt attggtgatc acacgagttt taccggaacc
    39361 cgcgcccgcc agcaccaggc aggggccggt aacgaattcg acagcttgtt gttggccggg
    39421 gtttagacgc ataggtgtat tgctcaatct tcgaacgggg ggaggattgt agcagaatcg
    39481 catttgaatg tgaccgcgtg taagccgcta accgattggc tgcggatgtt taaatcagag
    39541 agggaagacc gaaaccggag ttcatcgtga atccattaga cttagaaaat atcgggtcgt
    39601 aacgttgtgt tattactttt tcagagttta ctaaggagag gtgtactcgt ggcgattaat
    39661 ttcagtccta aggttggtga aatactggat tgcaactttg gtaactatcc tgttagccag
    39721 aatggacagt tcagcaccac gtattacgat ggccgcattc ctcctgaaat gataaaaaac
    39781 aggttagt
//
LOCUS       DAJTYZ010000021        39538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000021 DAJTYZ010000000
VERSION     DAJTYZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.021"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(9..84)
                     /locus_tag="OO596_001089"
     tRNA            complement(9..84)
                     /locus_tag="OO596_001089"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49..51),aa:Val,seq:tac)
     gene            complement(234..309)
                     /locus_tag="OO596_001090"
     tRNA            complement(234..309)
                     /locus_tag="OO596_001090"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(274..276),aa:Lys,seq:ttt)
     gene            complement(474..1265)
                     /gene="cpoB"
                     /locus_tag="OO596_001091"
     CDS             complement(474..1265)
                     /gene="cpoB"
                     /locus_tag="OO596_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6717002.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1275..1796)
                     /gene="pal"
                     /locus_tag="OO596_001092"
     CDS             complement(1275..1796)
                     /gene="pal"
                     /locus_tag="OO596_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6717003.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1831..3123)
                     /gene="tolB"
                     /locus_tag="OO596_001093"
     CDS             complement(1831..3123)
                     /gene="tolB"
                     /locus_tag="OO596_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6717004.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3256..4488)
                     /gene="tolA"
                     /locus_tag="OO596_001094"
     CDS             complement(3256..4488)
                     /gene="tolA"
                     /locus_tag="OO596_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6717005.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4553..4981)
                     /gene="tolR"
                     /locus_tag="OO596_001095"
     CDS             complement(4553..4981)
                     /gene="tolR"
                     /locus_tag="OO596_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6717006.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4985..5677)
                     /gene="tolQ"
                     /locus_tag="OO596_001096"
     CDS             complement(4985..5677)
                     /gene="tolQ"
                     /locus_tag="OO596_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6717007.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5674..6078)
                     /gene="ybgC"
                     /locus_tag="OO596_001097"
     CDS             complement(5674..6078)
                     /gene="ybgC"
                     /locus_tag="OO596_001097"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6717008.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYVPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(6228..6521)
                     /gene="ybgE"
                     /locus_tag="OO596_001098"
     CDS             complement(6228..6521)
                     /gene="ybgE"
                     /locus_tag="OO596_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6717009.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6521..6634)
                     /gene="cydX"
                     /locus_tag="OO596_001099"
     CDS             complement(6521..6634)
                     /gene="cydX"
                     /locus_tag="OO596_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6717010.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6649..7788)
                     /gene="cydB"
                     /locus_tag="OO596_001100"
     CDS             complement(6649..7788)
                     /gene="cydB"
                     /locus_tag="OO596_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6717011.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7804..9372)
                     /gene="cydA"
                     /locus_tag="OO596_001101"
     CDS             complement(7804..9372)
                     /gene="cydA"
                     /locus_tag="OO596_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6717012.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(9968..10195)
                     /locus_tag="OO596_001102"
     CDS             complement(9968..10195)
                     /locus_tag="OO596_001102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717013.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(10220..12776)
                     /locus_tag="OO596_001103"
                     /pseudo
     CDS             complement(10220..12776)
                     /locus_tag="OO596_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(12794..14710)
                     /gene="mngA"
                     /locus_tag="OO596_001104"
     CDS             complement(12794..14710)
                     /gene="mngA"
                     /locus_tag="OO596_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6717014.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14879..15601
                     /gene="mngR"
                     /locus_tag="OO596_001105"
     CDS             14879..15601
                     /gene="mngR"
                     /locus_tag="OO596_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6717015.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15705..16574)
                     /gene="sucD"
                     /locus_tag="OO596_001106"
     CDS             complement(15705..16574)
                     /gene="sucD"
                     /locus_tag="OO596_001106"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6717016.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16574..17740)
                     /gene="sucC"
                     /locus_tag="OO596_001107"
     CDS             complement(16574..17740)
                     /gene="sucC"
                     /locus_tag="OO596_001107"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6717017.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17834..19051)
                     /gene="odhB"
                     /locus_tag="OO596_001108"
     CDS             complement(17834..19051)
                     /gene="odhB"
                     /locus_tag="OO596_001108"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6717018.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19066..21867)
                     /gene="sucA"
                     /locus_tag="OO596_001109"
     CDS             complement(19066..21867)
                     /gene="sucA"
                     /locus_tag="OO596_001109"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6717019.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22168..22884)
                     /gene="sdhB"
                     /locus_tag="OO596_001110"
     CDS             complement(22168..22884)
                     /gene="sdhB"
                     /locus_tag="OO596_001110"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6717020.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22900..24666)
                     /gene="sdhA"
                     /locus_tag="OO596_001111"
     CDS             complement(22900..24666)
                     /gene="sdhA"
                     /locus_tag="OO596_001111"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6717021.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24666..25013)
                     /gene="sdhD"
                     /locus_tag="OO596_001112"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24666..25013)
                     /gene="sdhD"
                     /locus_tag="OO596_001112"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6717022.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25007..25411)
                     /gene="sdhC"
                     /locus_tag="OO596_001113"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25007..25411)
                     /gene="sdhC"
                     /locus_tag="OO596_001113"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6717023.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25792..25899
                     /locus_tag="OO596_001114"
     CDS             25792..25899
                     /locus_tag="OO596_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717024.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26105..27388
                     /gene="gltA"
                     /locus_tag="OO596_001115"
     CDS             26105..27388
                     /gene="gltA"
                     /locus_tag="OO596_001115"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6717025.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27778..28344
                     /locus_tag="OO596_001116"
     CDS             27778..28344
                     /locus_tag="OO596_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6717026.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28395..30853
                     /locus_tag="OO596_001117"
                     /pseudo
     CDS             28395..30853
                     /locus_tag="OO596_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            30868..31599
                     /locus_tag="OO596_001118"
     CDS             30868..31599
                     /locus_tag="OO596_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6717027.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31596..32657
                     /locus_tag="OO596_001119"
     CDS             31596..32657
                     /locus_tag="OO596_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6717028.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            32809..33855
                     /locus_tag="OO596_001120"
     CDS             32809..33855
                     /locus_tag="OO596_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6717029.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(33852..34643)
                     /gene="nei"
                     /locus_tag="OO596_001121"
     CDS             complement(33852..34643)
                     /gene="nei"
                     /locus_tag="OO596_001121"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6717030.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34679..35413)
                     /gene="pxpA"
                     /locus_tag="OO596_001122"
     CDS             complement(34679..35413)
                     /gene="pxpA"
                     /locus_tag="OO596_001122"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6717031.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(35403..36335)
                     /gene="pxpC"
                     /locus_tag="OO596_001123"
     CDS             complement(35403..36335)
                     /gene="pxpC"
                     /locus_tag="OO596_001123"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR6717032.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(36329..36985)
                     /gene="pxpB"
                     /locus_tag="OO596_001124"
     CDS             complement(36329..36985)
                     /gene="pxpB"
                     /locus_tag="OO596_001124"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6717033.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(37008..37751)
                     /gene="ybgI"
                     /locus_tag="OO596_001125"
     CDS             complement(37008..37751)
                     /gene="ybgI"
                     /locus_tag="OO596_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6717034.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38022..39503
                     /gene="dtpD"
                     /locus_tag="OO596_001126"
     CDS             38022..39503
                     /gene="dtpD"
                     /locus_tag="OO596_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6717035.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         9574 a        10638 c         9626 g         9700 t
ORIGIN      
        1 gacccgagtg gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg
       61 ctctcccagc tgagctaatc acccgatact gcgctggata ctacaaacta cttccaccga
      121 acccaccgta attagtggtg ggtcgtgcag gatgactcgc ttcgctcgcc ctacgggccg
      181 ttgctgacgc aacgttatcc tacacgctta acatctgtct tcgatgctaa aactggtggg
      241 tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc aactgctcta ccaactgagc
      301 taacgaccca ctttttcgtt gctttcggtt tgtttgatat cccgtggcaa cggcggcata
      361 tattactgat ttcagacttg agcgcaacaa aaatttcgat gcagatcact caactgctta
      421 tgattcgcac gacacgacca gaaataatgc gacttctggt cgtgtgttat gcattacatc
      481 gcgttcagac gtttttgtgc ctgtttagcg ccatcagtac cagggtattt actgataacc
      541 tgctggtata cggctttcgc ttttgcggtg tcacctttgt cctgcatgat gacgccgact
      601 ttaaacatcg catcagcagc ctttggtgac ttcggatagt ttttcactac cgaagcaaaa
      661 tagtacgccg catcatcttt tttacccttg ttgtagttta actgacccag ccaataattg
      721 gcgtttggca ggtaagttga atcagggtaa tttttgatga aattctgaaa tgccaccatt
      781 gcgtcatcct ggcgggattt atcctgcacc agcgcaatag ctgcattgta atccgtgttt
      841 gcatcgccgc ttttcaccgg cgcgccagca ttcgcagtac cagcatcagc tgtcggcgtc
      901 gttgatgccg ccgcaccgct ttgatcgctg ctggttgatt gcgccgctgc accaccgctg
      961 ctgaggctgt cgatctgcaa caggatctgc ttttgccgct ccacgacctg attcagttga
     1021 tactgatttt cctgaatctg accacgcagg gaatcaatat cggattgatt atcagaaagt
     1081 tgttgctgga gttgggttaa aagctggctg tgagcattag aaatacgctc aagttgagtg
     1141 acgcggtctt cgaccgagcc tgagccgaca ctactgattg gtgcctgagc aaaagcggcc
     1201 cagggggccg ctataccaac cagtaacgac agactcaata gttgatgtct gaagttactg
     1261 ctcatgcaat tctcttagta aaccagtacc gcacgacggt ttttggagta tgccgcttcg
     1321 tcatgaccca gtactgcagg tttttcttta ccgtaagaaa cgatggagat ctggtctgca
     1381 gaaacgcctt taccctgcag gtacatctta acggcgttcg cacgacgttc acccagggag
     1441 atgttgtatt ccggagtacc acgttcgtcc gcgtgacctt ctacggtgac tttgtaagac
     1501 gggttgctac gcaggaagtt tgcatgtgca tccagcattt gagcgaagtc agaacggata
     1561 tcgtacttgt ccagatcgaa gtaaacgatg ttgttctgct gcagctgttg catttgcaga
     1621 cgagcctgct cttcggaaga catgttgccg ttgccgccgt tcgcatccat accagtgccg
     1681 gcacccagca tgccttcgct gccgtcattg ctggcgttct tgttggaaga acatgccgca
     1741 attgccataa caggcagagc aatcatcagc cctttcagca ctttgttcag ttgcatttca
     1801 atgattcctt tactattcaa ttaattatta tcacagatac ggcgaccagg cagggaattt
     1861 gacctgtcca tcagttgccg gaagacgcgc tttgaaacgc ccatctgtag aaaccaaatt
     1921 cagcacggat cccatcccct gagaagagct gtagattacc atagtgccgt taggtgccag
     1981 acttggcgtt tcatccagga acgtggacga cagaacttgt acgcctcccg ttgccagatc
     2041 ttgtttggca atgtgctgct gcccaccgtt ggagctgacc attaccataa atttaccgtc
     2101 gctgctgaca tccgcatcct ggttctgcga accttcccag gtaatacgtt gtggcgcacc
     2161 gccgttgata ttcactttat aaacctgtgg acgaccggcc tggtcagaag taaatgccag
     2221 gttctggctg tccgggaacc aggtcggttc ggtattgtta ctgcgaccat cagtcacctg
     2281 gcggatctga ccagaagcca aatccattac gtacaggttc aggctaccgg ttttcgacaa
     2341 ggcgaatgcc agtttgctgc cgtctggcga gaatgcaggc gcaccgttgt gacgcgggaa
     2401 tgaagccacc tgacgtacag cgccgtttgc cagcgtttgg ataactagcg cggaacgacc
     2461 gctttcgaag gtcacataag ccagtttaga accgtctggt gaccacgccg gagacatcag
     2521 cggctgtggt gaacggtgaa cgacaaactg gttgtaaccg tcatagtcag atacgcgcag
     2581 ttcatacggg aactgaccgc cgttggtctg aacaacgtag gcaatacggg tacggaacgc
     2641 acctttaatg ccggtcagtt tttcaaacac ttcatcactg gcggtatgac cagcatagcg
     2701 cagccactgc ttgttcactt tgtacgagtt ctgagcaagt acagtacccg gtgcgccgcc
     2761 agtgtcaaca agttgataag caacattgta agaaccatcc ggattcggag tgacctgacc
     2821 gacaactaca gcgtcaatac ccagcgcgga ccatgcagct ggttgtactt cctgcgcact
     2881 acccggctgc tgtggcagac gagcgcgatc taacggatta aatttaccgc tgttacgcaa
     2941 gtcggcagca acgatgccgc caatatcttc aggtgccgca ccagggcccg cccactggaa
     3001 aggaacaaca ccaataggac gaccggaatc tacaccgctg tcgatcacaa tgcggacttc
     3061 agcatgcaga actgatgccc acagtatgag aaaaccaaat gctactcgta atgcctgctt
     3121 catcatatct cccttatctg gaccgatggc ccacgataat ttagcagaat gttaacaaac
     3181 taaaatacac aaaactacca gaaccccgtg gcaaccggtg cctgatgttg accgtcggaa
     3241 cagtcaacat cgcgattacg gtttgaagtc caatggcgcg tttttgaaca cttcatatac
     3301 tgcctggctt ggtggtttcg ggatcttcgc aagtttagct gctgccaacg cagcctgaca
     3361 aagtgcggga tcgccacctt caggtttgat atccagtaac ataccatcgg gtgccagttt
     3421 tatgcgcagc gtacaggttt tgcctgcata ggacgatgcg tcatagaact tactttcgat
     3481 agcagattta atctgcccgg catagttatt gatatcggcc cctgatgcgc cattgttttt
     3541 agtattacca ctcccggcag acgaagcatt gttccctttc gcccctcccc ccgttttcgg
     3601 tgcattctta ccagagctta gctcaccgaa aatatcatct gcctctgcgg cagcttttgc
     3661 tgcagcggct ttttcagctg ctgcttttgc cgctgccgct tttttgtctg cggcggcttt
     3721 ttctgccgct gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc
     3781 tttcttatca gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc
     3841 agctttctct gctgccgctt tctttcttgc ttcagctgca gcagcttctg ccgcttccgc
     3901 tttcttcttc agtgccgcag cggctgcctc ggcttttttc tgcgcttcgg ctgcggcttt
     3961 ggcggcttct gcttctgctt ttttcttcgc gtctgccgcc gctttcttcg ctgcttcttc
     4021 cgcagcttta gcatccgctt cggccttcgc tttagcatct gccgccgctt tcgctgccgc
     4081 ctcttccgct tgcttctgct ttaactcggc ctgttttgcg gcttcttcag cctgcttttt
     4141 ctgttcctga gcagctaacc gctctttctc aagttgcttc aggcgttcct gttcagccgc
     4201 ttgtttctca cgcagttctt cagcagcctg ctgttccttt atcttgcgct gctcatcaga
     4261 acgcttcgcg cttgattcct ggctttgcat gcgtttgtac tgctcaacta ccgcacctga
     4321 atcaaccatg acagcgtcga tggacgaacc accgccgcct ccggctgaag cttctatatt
     4381 ctcatcgaac gaactccaga gcagcgccgc aaataagatg acatgcagca ctgctgaaat
     4441 aattatcgcc cgcttgagct tgtcgttttg ttcggttgcc tttgacactc tcggtttcca
     4501 aaaactgttc gcctgttacc cgctctcttt caagcaaggg aaacgcagat gtttagatag
     4561 gctgcgtcat taaaccaacc gatttcacac ccgcactatg taacaagttc agtgctttaa
     4621 ttatttcatc gtaaggcaca tcttttgcgc caccgatcag aaagaccgtt ttcgggttgg
     4681 ccttgaaacg gctggacact tccgccacca cctgctctgg tggtaaacgc tccaggcgat
     4741 ctttctcaac caccacggtg tactgaccaa taccagacac ttcaacaatc actggcggat
     4801 tatcgttact gctcaccgcc tgtgattcag tagcgtctgg cagatcgacc tccacgctct
     4861 gggtgatgat gggcgctgtc gccataaaga tcagcaacag caccagcagt acgtccagca
     4921 acggtacaat gttgatttcg gacttgagat cgcgacgacc tcgtccacgc gctctggcca
     4981 tggcttaccc cttgttgctc tcgctaacgg taaacgcctg gcggtgcaga atcgcggtaa
     5041 actcttccat aaagttgtcg taattcagtt ccagtttgtt tacgcgctgg ttgaggcggt
     5101 tataagccat taccgccgga attgcggcga acagaccaat tgcagtcgca atcaacgctt
     5161 ctgcgatacc gggcgcaacc atttgcagtg ttgcttgttt taccgccccg agggcgataa
     5221 aggcgtgcat gatcccccag accgtaccaa acagaccaat atacgggctg atggagccaa
     5281 ccgtaccgag gaacggaatg tgcgtttcca gattttcaag ttcacggttc atggagatac
     5341 gcatagcacg cgacgcccct tccactacgg cttccggcgc atggctattg gcacgatgca
     5401 ggcgcacaaa ctctttgaac ccgctgtaaa atatttgttc cgaaccagtc agattatccc
     5461 gtttcccctg actctcttga tagaggcgag agagttcgat tccagaccag aatttatctt
     5521 caaacgcttc ggcttcgcgc gccgctgcgt taagaatacg ggtccgctgg ataataatgg
     5581 cccaggatgc gattgaaaaa ccaatcaaaa tcaacatgat aagtttaacc agaaggctag
     5641 ccttcaggaa caaatcaagg atattcatgt cagtcactgc ttaaactccg cgacaataga
     5701 cttgggaagc gcacaaggct tcattttgag tgggtcaacg caaacaacca gaacctctgc
     5761 ttcattcagc aaagtattct cggcgttgac aatacgttgc gtgaaaacca aagaggtgcc
     5821 acgcattgat gttatttcag tctgtatttc gagcatatcg tcgagccgcg caggtacgta
     5881 atattccacc gtcattttac gtaccacaaa ggcaacgcgt tcagccatca gcgcctgctg
     5941 actgaagtga tgatgacgca gcatctctgt gcgtgctctt tcataaaaag cgacgtaact
     6001 ggcgtggtac accacaccac cggcatcggt atcttcatag tagacgcgaa ccggccatcg
     6061 aaacagcgtt gtattcactt tacatcccgg taatgcaaca aaagttagag cttttaaacg
     6121 ctgctactat acgcgcgcga ttgaggtttg ggaatgaggg caagttaagg gagcgtaaaa
     6181 atttatgggc ctggagcggc ccataatttt ttaaagaaaa ttctgattta aaagaagaag
     6241 aaaatcagcc cgacaatgag aacaatatcg gcaagcaatg ggcaaaaaat cccttgccaa
     6301 agaaccttct gcggacgaaa gcccacgccg tgaatcacac cagcacagac ggcccacatc
     6361 agcaataaac catgccagat ttccagttca ctggtcttcg cggcaaaacg agatgggtcc
     6421 caaaacatac atcctgctaa cagaagcgcc atcacgaagg aaagcgcccg cagggggcgc
     6481 ttgtccatta ccgcatacaa ggtcgcgata atcttactca tcagatgtct tcttgaccgg
     6541 ctttgcctga ttcgacgtgt tcaagcgcca gcgcggtgat taccccaaac gaacaggcaa
     6601 gaagcgttcc cagaatccat gcgaaatacc acatttttag ctccttactt agtacagaga
     6661 gtgggtgtta cgttcaatat cttctttggt gatacgaccg aacattttcc agtaacacca
     6721 agcggtgtag agcagaatga tcggtaccag aaccaccgca acccaggtca tgacgttaag
     6781 cgtcagctgg ctggaagttg catcccacat tgtcagactt gcgttcatca tggtgctgga
     6841 cggcatcaca aacgggaaca ttgcgatacc ggctgtcagg atgatgcagg ccagcgtcag
     6901 ggaggagaac acaaacgccc acgcggcttt atccatacgt gcagtcagga tggtcagcag
     6961 cggaagaacc acacccagtg ccggaatagc ccacagaatt ggcgtgttgt tgaagttaac
     7021 cagccatgcg ccagcttcac gaaccacttc tttattcagt gggttagagg ctgcgtaatg
     7081 gtccattgtc gatttcacga cataaccatc gataccgtac atcacccata cgccagccag
     7141 tgcgaaacag accagtgtca ccagcgcagc cacctgagcc gttgcacggg tacgcaggtg
     7201 cagttcgccc acggtacgca tttgcagata ggttgcgccc tgagtaatga tcatccctac
     7261 gctcaccacg cctgccagca ggccgaacgg gttaagcaac tggaagaagt taccggtgta
     7321 gtacagacgc agatattcat caacgttgaa cggtacgccc tgcaacaggt taccgaacgc
     7381 tacaccaatt accagcggcg gaacgaaact accaatgaag atgccccagt cccacatgtt
     7441 acgccagcgg gtttcttcaa tcttggagcg gtagtcaaaa ccgaccggac ggaagaacaa
     7501 agacgccagc acgaggatca tcgccacata gaagccggag aacgcagcgg catagaccat
     7561 cggccaggca gcaaagagtg cgccgcccgc ggtgatcagc caaacctggt taccgtccca
     7621 gtgtggtgca atggagttaa tcataattcg acgctcggtg tcgttacgac cgaggaaacg
     7681 ggtgagcatg cccaccccca tgtcgaaacc gtcagtgact gcaaaaccaa tcagcagaac
     7741 gccaaccagc agccaccaga taaaacgcaa tacttcataa tcgatcattt gacgactcct
     7801 gtcttagcgt gccggctgag tagtcgtgga agactgctca aagtgatagc gaccggtttt
     7861 caggctgctt gggccgaggc gtgcaaactt gaacattaag aacaattctg ccaccaggaa
     7921 cagggtatac aggccgcaaa tcagcaccat tgagaagatg agatcgcctg cggtcagtga
     7981 cgagttcgcc acagctgtcg gcagcacttc accgatagcc cacggttggc ggccatattc
     8041 agccacgaac cagcccgctt ctacagcaat ccacggcagc ggaataccgt acagcgcggc
     8101 gcgcagaagc cattttttct cgccaatgcg gttgcggatg acactccaga aagagagcgc
     8161 gatgattgcc agaagcagga agccacacgc caccatgata cggaacgcaa agtacagcgg
     8221 cgctacacgc gggatggagt ctttggttgc ctgttgaatc tgcgcttcag tcgcatcagc
     8281 cacgtttggc gtatagcgtt tcagcagcag accgtaaccg aggtctttct tcatgctatt
     8341 gaactggtca cgaaccgcct ggtcggtaga accagaacgc agttgttcga gcagagagta
     8401 cgccttcatc ccgttacgaa tgcgttcttc atgctgcacc atcagctctt tcaggccgat
     8461 aaccggggta tccacggaac gcgttgcaat gatgcccagt gcgtaaggga tctgaatcgc
     8521 aaatttgttc gtctcctctt cctgatcagg aatgccgaac agagtaaagg cagcaggcgc
     8581 aggttgcgtt tcccactcgg cttcaatagc agccagtttg gttttctgca cgtcgcccat
     8641 ttcgtagccg gattcatcac ccagaacaat aacagacaga acagcagcca taccgaagct
     8701 ggcagcgata gcaaaggagc gtttagcgaa ggcgaagtca cgacctttca gcatatacca
     8761 tgcgctgata ccgaggatga acatcgcgcc agtcacataa ccagacgcta cagtgtgaac
     8821 gaatttcacc tgagcaaccg ggttaagcac cagctcggag aagctcacca tctccatacg
     8881 catagtttca aagttgaaat cggacgcgat tgggttttgc atccagccgt tcgcaaccag
     8941 aatccacagt gcggacaggt ttgaaccgag cgccaccagc caggtgacac acatatgctg
     9001 aactttaccc agacgatccc aaccgaagaa gaacagacct acaaaggtgg attcgaggaa
     9061 gaaggccatc agaccttcga ttgccagcgg cgcaccgaag atatccccta catagtggga
     9121 atagtaagac cagttagtcc cgaactggaa ctccatggtc agaccggtag ccacacccag
     9181 agcgaagttg ataccaaaca acttgcccca gaacttggtc atatctttat aaatctgttt
     9241 gccggagagg acgtagaccg tttccataat ggccagcagg aacgccatac cgagcgtcag
     9301 tggcacaaaa aggaagtggt acatcgcggt caaggcaaac tgtaagcgcg acagttcgac
     9361 tatatctaac atcatgactc cttgctcatc gcatgaagac tccgagagtg aaccccgtta
     9421 gaaagggtca cacgcatgcc ccaatagaaa tttgtttgaa cactttcatc ataccatgtg
     9481 aatgcacaca aaaacggcgt aaagaaaggt tgcaaacatg ttaataaaaa ctcaaattga
     9541 tcccacgtat atattacgct gcaaaatcct tacaataaac aggtttttgt tgaagcaaat
     9601 ttgcattttt cgacggtgat caatttatag ctaaattacc gcctttcagc caatttgatc
     9661 gagaacaatt tatctctttt tgatgccaat ttccaagact tatacattga taaatatcaa
     9721 ttcctacaaa ttatgttaat catgtttcaa taaggtaact ttagtgacat ttatgtttaa
     9781 aatgtgtgag ttataaggct gaaattagtt tcggttggca atttaaagat aaaaagagac
     9841 gaagcaaaaa cagcacggtt gaccttaatt ctttttttgg tcactggcta atacgacgca
     9901 gagtttcagg attactaata aaggcataaa tccaccccgc cgatattaac atttaattaa
     9961 cttgtaatta tattataatt aactcaccca gggttattat tttgttaaaa ataaagccac
    10021 atatacgtgg cttgagagtg aagataaacg caaaattacc tgatgcgcta cgcttgccag
    10081 tgtctacatc atctctgcaa tatattgaat ttgcctgctt ttgtagaccg aataaagcgt
    10141 tcatgccgta tccggcatga acaaagcgcc ctttgtcaac aatctggccg cgcatatgtg
    10201 gcttcggttt gattgctatt caggcaagcc ggtaactgaa cgtccgtgac tggccgggta
    10261 aaaaaggccc cgatagattt gaaccttgat tttcctcggt ggtaatgtgt tcatccatca
    10321 tcgtttctga gcaagaaatc acctcgcgac tgaaagcaac agtcgcatca caggttgctg
    10381 attcagccgg attaaacagc cgtaaaatca cttcttgtcg gtcttcagct ttcttaagtg
    10441 cgcttatcag gcatcccact gggggcattt tcaacaaact ataactttcc ggcacgttga
    10501 atccggcttt gttgagcttc atcgcatccc atgggatttt gttgtaacac tgtactggag
    10561 tcagccatgc tcgcgcctgc tgagctacac cagcggcggt tggcgtaccg gtataactca
    10621 ataaacttag gcgacaagaa agcagaccac gtagttgtga gtccgggact ggcattttaa
    10681 ttcccgaagg ccgcccaggc cttaaaagca gatcttcttt gcccagtaag cccacgccac
    10741 gcagcaacgt aatggcaaag gttttcttct cttcaccgat gacttcaaat tcacgtaacc
    10801 cttcgctaaa gacagccatg ccgttacgcc cttcctgtaa ggcaacatag ttgagcatat
    10861 tccataccgg aaccggcgct tctttccagc cttcttgctg ccagttgttc attgcactgt
    10921 cgttcacggg gcgcgttagc gaaccaaact gcgtatctgc cagaacactg tcagtgttaa
    10981 aaggtgtagg gaccaggaca cgaaggcgat gatcgtcagc ctggttatca aggttgatat
    11041 ccacatcaat acgcctgctg ttatgactaa gagtgacaac caacaccccc cctactctgc
    11101 caggcttcat gaataatatc gcattgcggt ttcgcgttcg ctgcggtaat tacccactct
    11161 tcttttgcgg gtgaatagtc atattcatca ccatcatcag agctttcttc aatttgcaat
    11221 acccggtcat agcgcacacc gctgtcttta tctaccagtt gcagagaacc atcctcattg
    11281 agcgcaatct gccagaaagc attttccaga atcccttcag cgtcactttt tgccgcaatc
    11341 acgttgccag gctgattcgc ttcaatataa agcgtgcgat agcccataga agggacaatc
    11401 tggttgatct gaatatcaaa ctccataaag ggatcataat tgccataatg aacaatttgc
    11461 cgatcgatta ggccaggatc gatctcacgg gcatggcgaa taaaatacgg tacaggctga
    11521 ccgcgatcgt cttgtagatt aaactggctg gcgcgcagcc gcacagtggt gttaataact
    11581 tcttcccgcg gccagggcat caggttaaac agcacgagtt tgtcggcatc actctgtggc
    11641 atgttatcgg caattttgcg catgtagaaa cgcagcagat tatccgccat gtcttcagcc
    11701 agttcgaagc gggcgacaat ttcgcgatga actttgtcac tgcaacaaca accgatactg
    11761 tcgtgggcat gattttttaa gatctctttc cacattttct ccagcaagcc gtggtggtat
    11821 tcaaaaccca acgtccaggc cagcgttgcc agcggttcca gcagattaac aatcttattt
    11881 tcaatacgtg cgtgggcaat tttgatatcc atgcgcgtgg aaccgatggt gcgatgtacg
    11941 cgcatatatt tgccatcgat aaattcacct ttcagggtgg ccagactttc acgctgcgct
    12001 tcgatcttct caaatacctc ttcaaagcgg ctcatcacaa acttacgttg agggtagatc
    12061 tcacgtagct tatccatcac ttcgaagata ttttgctgca atggcatctg gtcatgacca
    12121 ttcggcagca aaatctcttt ggttacagac gctttttcca gcacgtcaaa ataactgtcg
    12181 aggcgtttac gtaatccgtt ttcgtcggca ggtaagtact taccgatggc gtagcccagc
    12241 ggcagcacct gcgccgtcac ttcgctaccg tcactgcttt gccacaaaaa ctcggtttta
    12301 tcagtaccgt ggcgctccga acatccgcgc cagaacatag tgcgggtaat gccaaatcca
    12361 ttgtaaatat gcggcagttg cccggacatg ccaaaggaat ccggtaaata acctattttc
    12421 atcggctcgc caaacgcgag gcagtcacgc attccgtaca tcagattacg gacgatggat
    12481 tccgcagaaa caatcgtggt atcggtctgg gtataccagg ggccgataat caacttaccg
    12541 gcttctacct gtttcttcac gcggtctttg ttttccggtt tcaccgcgaa ataatcttcg
    12601 aggatcgccg tttgcccgtc gagtacgtaa tatttgtatt cgttgtcctg ttccagtcgg
    12661 cacaggatct cttccatatt attgaccagc agaatgcgtg actcttcggt ggtgaaatac
    12721 cactcccgat cccaatgcat atgcggggtg atgtgaacgc gagataccgc tttcatcgtc
    12781 gtttcctttt tgtttatggc attacgccat cagtcagata gttgccatgc ttaaccgcgt
    12841 gacgccgcca gatcaggaga attgcagtcg agattgcagc ccccaccaat gccgcaccaa
    12901 accagccaat tgccgccata acaccgcccg cgccattatc atgaagtaaa aagagcgaga
    12961 aaatgccggc accgggtgtc gaaaggccga tattcatcgc accgacaata gcgcccgtta
    13021 ccatagagcc cagcacaaac gaaccaataa cccgcagcgg atcttcaatc gccatcggga
    13081 tcgccccttc ggtaatacct gccagcccta acagccaggt ggatttcccg gtctcaattt
    13141 caaactcttt aaacaggcgc ggtgcgagca tcgtggaagc ggttacggta aatgccgaaa
    13201 ccattttgac ggaggcgaaa atggcatacg ggccgtaaac gccgttcgcc attgcgccca
    13261 ggcagaatgc ataagcggct ttgttcactg gccctccaag gtcaaaggaa cacataaaac
    13321 cgagaatggc acccagcaac agcgcgttac ttcctgacag accgttcagc caggcggtaa
    13381 gcgagttatt gatccatgcg acaggttctc ccaccacaaa cagcatcaga ctgcccgctc
    13441 ccaacgtacc gagcaccggg tagagataaa aagtcaggaa tccattgaat ttactgctaa
    13501 gacgcaagtg atttttcacc cagcgcatca agtaaccggc tatcaatccg ccaacgaccg
    13561 cgccgagaaa cccggagccg atcatgttgg cggcaagtcc agccgcaaag cctggcgcta
    13621 acgccggttt atctgccaga gaataggtgg tataggccgc gagcaccggt accatcaaaa
    13681 ttccgagcag cccgccgccc agcttgcggt acatccacag ccaggagttt tcttcattaa
    13741 acagatcttg tagcccgaag atttgcgaca gtaataccgc gaccgccagc accgtgcccc
    13801 ccgcgacaat caacggtacg gcaaaagaga ttccgctcaa cagtgcctgt ttcagctccg
    13861 ttttgacact tttcaccggt tgcgtatctt gctgtacggt acgcgtctca tcgctacgct
    13921 tgagggtaag cgcttgttgg atcaacgctt ctgcatggcg aatcggctcg gcaacaggca
    13981 ctgaaagcgc gggaatgcca ttaaaacgct cactctcctt gatggcgact tcagccgcaa
    14041 aaatacaggc ggttgcacta ttgagttgat ccgccgttaa acgcccttca atgccgttag
    14101 cgccttgttt ttcaacgtaa acatttacgc cgagtttgcg tccggctttt tccagatatt
    14161 ccgcagccat ataggtgtga gcaatacccg ccggacaggc cgttacgcag acgatagttg
    14221 gcgcgttgga aaaagaggca gaaggttgcg tgcctccctt gtcatccagc gccgagagca
    14281 actcatcagg cgtcgtcgcc gactgtatac gcgcccgaat ctcatcatcc gcaaggcgcg
    14341 tggtcagcgc tgtcagcagt tgcatatgcg tagtacccgc ttcattgggg ggaatcgcca
    14401 gcagcaccac taaatcaact gcttccgggc catcaacgcc ttcccactga agcggctcgc
    14461 tgagtgtggc gaccgcaaac gccgcttctt ttaccgcagc agttttgcca tgcggcacag
    14521 ccaacccttc acctaaggcg gtcgggccaa ggctttcacg gcgatacact tcttccagaa
    14581 attgctcagt actggaaatt ttccccagag cagcaagacg ttgagtcaac gcgtggatgg
    14641 cctcttcacg gctggtaaag cgcgcattca gacacaacgc atcgcggtgg gtcagagtcg
    14701 tcagattcat catgatcctc acttgatcgt ttttatagtc gcgccagtgt gcccgataaa
    14761 acaataccat catgtgatca atttcacttg attgaaatta tttgtattgt atttgtatta
    14821 tttacaggat gaatttatcg ggcataatgg ctaaattcat attaatgaga tactaaaaat
    14881 gggacacaag cccttatacc ggcagattgc cgatcgcatt cgtgaacaaa ttgcgcgtgg
    14941 cgagcttaaa cctggcgatg cgttacccac tgaatcagcg ctacaaacag agtttggcgt
    15001 cagccgggtt acggtgcgtc aggctttgcg ccagctagtc gagcagcaga tcctcgaaag
    15061 cattcagggc agcgggactt acgtcaaaga agagcgggtc aattacgata tttttcagtt
    15121 aaccagtttt gatgaaaaac tgtcggaccg tcacgtcgat acacacagtg aaatcctgat
    15181 atttgaagtg attccggctg acgattttct tcagcaacag ctacaaatca ccgcgcagga
    15241 tcgtgtgtgg catgtgaagc gcgtgcgcta tcgcaagcaa aagccaatgg cgctggaaga
    15301 aacctggatg ccgcttgcgt tgttcccgga tctcacctgg caggtcatgg aaaattcgaa
    15361 atatcacttt attgaagaag tgaagaagat ggttatcgat cgtagcgaac aggaaatcat
    15421 tcctctgatg ccaaccgaag agatgagccg tctgcttaat atcagccaga caaagccgat
    15481 tcttgaaaaa gtatcgcgtg gatatttagt tgatggtcgg gtgtttgaat atagccgcaa
    15541 cgcttttaat accgatgact ataaatttac cctgatagcg cagcgaaaat catcgcgata
    15601 agcacaaaaa aggccatcat acgatggcca accatgtcga aaccgacagc ccttcgccat
    15661 tcctgtagag ggaagcggcg agggctattt cttattacag atatttattt cagaacagtt
    15721 ttcagtgctt caccgatatc cgccaggctg cgaacggttt tcacgcctgc ggcttccaga
    15781 gcagcgaatt tctcatccgc agtccctttc ccaccagcaa tgatcgcacc cgcgtggccc
    15841 atacgtttgc ctttcggcgc agtcacacca gcgatgtaac ctacaactgg cttggtaacg
    15901 tgctctttga tgtacgctgc cgcttcttct tcagcgctac cgccgatctc accgatcatc
    15961 acgatcgctt cggtctgcgg atctttttcg aacatttcga ggatgtcgat aaagttagag
    16021 cctgggatcg ggtcaccgcc gataccaaca caggtcgact gaccgaaacc gtaatccgtg
    16081 gtctgtttaa ccgcttcata ggtcagtgta ccggaacggg aaacgatacc cactttaccc
    16141 ggtttgtgaa tgtgacccgg ctggataccg attttgcatt cacccggagt gataacgccc
    16201 gggcagttcg ggccgatcat acgaacgccg gcttcgtcca gtttcacttt cacagtcagc
    16261 atatccagcg tcgggatgcc ttcagtgatg gtgataatca gtttgatgcc tgcatcaata
    16321 gcttccagaa tggagtcttt gcagaacggt gctggtacgt agataacaga agcggtagcg
    16381 ccagtagcag caacagcttc acgcacggtg ttgaacaccg gcaggccgag gtgggtagta
    16441 ccgcctttac ctggggttac gccgccaacc attttagtgc cgtatgcaat ggcctgttct
    16501 gagtggaaag tcccctggct accagtaaag ccctggcaga taaccttggt gtttttatca
    16561 attaaaatgg acattatttc ccctccactg cggcaacaac ctgctgagct gcatccgtca
    16621 gaccttttgc tgcaataata ttcaggccgc tgtcagccag tttcttcgcg ccgagttcgg
    16681 cgttgttacc ttccagacgt accacgaccg gtacgttaac acccacttct gctaccgcgc
    16741 cgatgatacc gtcagcgatc aggtcgcaac gaacgatacc gccgaagatg ttaaccagaa
    16801 cggctttcac tttgtcgtca gagaggatga ttttgaacgc ttcggttaca cgttctttgg
    16861 ttgcgccgcc gccaacgtca aggaagttag ccggttcgcc gccgtgcagt ttaacgatgt
    16921 ccatcgtacc catcgccaga cctgcgccgt taaccataca accgatgtta ccgtccagcg
    16981 caacgtagtt cagttcccac tgtgcagcct gtgcttcacg cggatcttcc tgcgactggt
    17041 cacgcatttc gcgcagatca ggctggcgga acagtgcgtt gccgtcagcg cccagtttgc
    17101 cgtcgaggca aatcagatcg ccctgtttgg tgatgaccag cgggttgatt tcgatcaacg
    17161 ccaggtcgcg ctccaggaaa atggtcgcca ggcccatgaa gattttggtg aactgctgaa
    17221 ccagtttacc ttccagaccc agtttgaacg ccagctcgcg tccctgatac ggcatcgggc
    17281 cagtcagcgg atcaagcgca actttgtgga tcaggtgcgg agtttcttcc gccacttttt
    17341 cgatttccac gccgccttcg gtggaggcca taaagaccac acgacgggaa ctacggtcta
    17401 caacggcacc gagatacagc tctttagcga tatcggtcgc tgcttcaacc agaatctggt
    17461 taaccggttg gccattggca tctgtttgat acgttaccag acgcttgccg agccagtttt
    17521 ctgcaaaagc acggatgtct tctttgctgt ttacaacttt cacaccgccc gctttaccgc
    17581 ggccaccagc gtgaacctga catttcacta cccacgggcc ggcaccgatt tttgaagcgg
    17641 cttcttctgc ttcgcgcgga gtagtacagg cataacccac cggtgctggt aagccatagc
    17701 gggcaaaaag ttgttttgcc tgatattcat gtaagttcat gtgttctgtc catccttcag
    17761 taatcgttat cttttaaacc gtagaccgac accctatcca cgtcggtcta cagtgcaggt
    17821 gaagcttaaa ctactacacg tccagcagca gacgcgtcgg atcttccagc aactctttga
    17881 tcgttaccag gaagcccacg gattcgcgac catcgatcag acggtgatcg taggacagcg
    17941 ccaggtacat catcggcagg atctcaacct gaccattcac cgccatcgga cgatctttga
    18001 tagcgtgcat acccagaatt gcgctctgcg gcgggttgat gatcggcgta gacatcaggg
    18061 aaccgaacac accaccgttg gtgatggtga agttaccacc ggtcagatct tcaaccgtca
    18121 gcttgccgtc acggccttta actgccagct ctttgatttt cttctcgatg tctgccatgc
    18181 cgagggtatc gacatcacgc agtaccggcg tcaccaggcc gcgcggcgtg gaaaccgcca
    18241 tgctgacgtc gaaatagttg tggtaaacca cgtcatcacc gtcgatagaa gcattcactt
    18301 ccgggtaacg tttcagggct tcaaccaccg ctttcacgta gaaggacata aagcccagac
    18361 ggatgccgtg gcgtttttca aacgcttcac cgtactgctt acgcagatcc ataatcggct
    18421 tcatgttgac ttcgttgaac gtggtcagca tggcggtgga gtttttcgct tccagcagac
    18481 gctctgccac acgcttacgc aggcgagtca tcgggacacg tttttcacta cgtgcagcca
    18541 gagccggttg cgccgccgga gcagccgctg ccggagcaga ctctttcgcc ggggctttcg
    18601 ccagatgttt ttccacatct tcacgagtca gacgaccacc cacaccggtg cctttaatgg
    18661 cgctggcgtc gagattgtgt tcagccagca ggcgacggat cgccgggctt aacgcatcgt
    18721 tgttttgctc ttccagagac gcctgctggc gttgcgccgg agtggacgct ttctcttcag
    18781 atttggcgct ggtttcttta ccggcgctgt tgccttcacg caggcgacca aggatctgac
    18841 gagacgttac cgttgtacct tcatcttcca gaaccgcatc cagaatgccg tctgctgatg
    18901 ccggtacttc cagtaccact ttgtcagttt cgatttctac cagcacttca tcacgtacga
    18961 ctgcgtcgcc gggtttttta tgccaggttg cgacggtggc atcggctacg gattcaggca
    19021 ggtcagggac cagaatatct acgctactca ttgtgtatcc tttatttatt cgacgttcag
    19081 cgcgtcatta accagatctt gttgctgttt ctggtgaacg gacatatacc ctaccgccgg
    19141 agaggcggag gccgtgcggc ctgcataacg cagagaagcc ccaaacggaa tcacttcacg
    19201 gaaatgatgc tggctgcagt accatgcgcc ctggttgagc ggctcttcct ggcaccagac
    19261 aaaatccttg acgtgagcaa actgctgcaa cacttcctgc atcgctttat gcgggaacgg
    19321 gtagagttgc tcgatacgca caatggcgac atcgtgttga ttgttcttac gacgctgttc
    19381 cagcaggtcg taataaacct taccagaaca catcactacg cgcttcacgc ccttcggatc
    19441 aagctcgtcg atttcaccga tggctggcag gaaggtgccg ttcgccagtt cttcgaggct
    19501 ggaaaccgcc agcggatgac gcagcaggga tttcggcgac atcactacca gcggacgacg
    19561 catcccgcgc agcgcctgac gacgcagcat gtggtaaacc tgtgccgggg tagacggaac
    19621 gcaaacctgc atgttttgct cagcacaaag ttgcagataa cgttccagac gcgcggagga
    19681 gtgctccggc ccctgccctt cgtaaccgtg cggcagcaac atcaccagac cacacatccg
    19741 gccccatttc tgttcgccag aagagatgaa ctggtcgata accacctgcg caccgttggc
    19801 gaagtcaccg aactgcgctt cccagatggt cagagtgcgt ggttctgcgg tggcataacc
    19861 gtattcaaac gccagcactg cttcttcaga cagtacggag tcccagacgc ggaacgcgcc
    19921 ctgcccgtta tggatgtgtt gcaacggcgt gtaagtggaa ccgttagact ggttgtggat
    19981 caccgcgtgg cggtggaaga aggtaccgcg accggagtct tcacccgaca ggcgaaccgg
    20041 aatgccttcg tcaaccagcg tggcgtaagc gaggttttcc gcaccgcccc agtcgaacag
    20101 tttctcaccg gcagccatcg cctggcgatc gccataaatc ttggcaacgc gagactgcat
    20161 ttcaactgct tccggcaccg tgctgatgcg tttcgccagc tcctgcaggc gcttcatctc
    20221 aactttgttc gggtactctt cgtcccattc gtggttgagg tacggcgacc aggtgaaaga
    20281 gtgcatgttc atcggacgcc actctgccac tacgcaatcg ccagcatcca gcgcatcgcg
    20341 gtacaggtta accatctcgg tggcatcttc cagcgtcgcc actttttcct gctccagctt
    20401 gtcagcgtag attttgcgcg gtgtcggatg ttttttgatt ttctgataca tcagcggctg
    20461 ggttgcgctc ggctcgtcgg cttcgttgtg gccgtgacgg cggtagcaca ccaggtcgat
    20521 gaagacatca cgtttaaagg tgttacggaa atcgagcgcc agacgggtca caaaggcaac
    20581 ggcttccgga tcatccgcgt taacgtggaa aatcggtgcc tgaaccatct taccgatatc
    20641 agtacagtac ggtgtagaac gagcatccag tggattagag gtggtgaaac caacctggtt
    20701 gttgataacg atacgtaccg taccgccaac ttcataacca cgcgctttcg acatgttcag
    20761 agtttcctga accacgccct gcccggtcac tgcggcgtca ccgtggatgg tgattggcag
    20821 cactttgttg ctgctcggct catcaagtct gtccagacgg gcacgaacag aaccgataac
    20881 taccgggctt acaatctcaa ggtgagacgg gttaaacgcc agcgccaggt gcaccaggcc
    20941 gccatcggtc tggaagtcag acgagaagcc catgtggtat ttcacgtcac ccgtgccgag
    21001 gtgttcttta tgtttaccgg caaactcgtc gaacaagtct tgcggttttt tacccagcac
    21061 gttcaccagc acgttcagac gaccacggtg cgccatccca agaaccactt cgcgggtgcc
    21121 gctgttgcca gcgtggcgga tcatctcttt aagcatcggg attaacgcgt caccgccttc
    21181 cagcgagaag cgttttgcgc cagggaattt tgcgccgagg taacgttcaa ggccttcagc
    21241 ggcggtcagt tcgcttaaga agcgtttttt ctcttcgcta ttgaaagtcg cgcgaccaga
    21301 ctcgatacgc tgttggatcc agcgtttttc ttcggtgctg gtaatgtgca tatactcggc
    21361 accaatcggg ccacagtagg tttgcttgag ggcttccagc agctcgccga gtttcatgtt
    21421 ttctttgccg ctggcaaatg aaccgacgtt gaaggtctcc tggaagtctg cttcggtcag
    21481 atcgtggaaa gacggatcca gatcggccac tttatcttgc tgccacagtc ccagcggatc
    21541 gagattcgca tgctggtgac cacggaagcg gtatgcgtta atgagctgca ggactttaac
    21601 ctgcttcaca ttggtgtcag ggtcggagat cgttgaagag taacgtgaag cgtctttcgc
    21661 caggcggcgg aaatattcac gcgtttgaga gtggaattga tccggtttga ctcccgtacc
    21721 aggtaactgc tggaacgtcg aacgccagtt agcgtcaacc gagtcaggat cggttaagaa
    21781 gtcttcatag agctgttcta tccagctctg gtttgcgcca gagaggtaag aagagtccaa
    21841 ccaggctttc aaagcgctgt tctgcatcgt gatcccttaa gcatcttttt tatgcttact
    21901 tcgccgtgga tactaccacg cacagtgatg tgcgtgccgg ggttcacttg cgagctcttg
    21961 tctatgtagt ggctcgcgaa ggaaccttta aaaactgtct tagtgtcagg ggcagttttt
    22021 aaagatttcc tgcatttctt tgttacgcct gatgcgctgc gcttatcagg cctacgggta
    22081 atgacttgta ggccggataa ggcgttcacg ccgcatccgg cactggttgc ctgatgcgac
    22141 gcttgcgcgt cttatcaggc ctacggttta cgcattacgt tgcaacaaca tcgacttaat
    22201 atggccgatg gcgcgcgtcg ggttcagccc cttcggacat acactgacgc agttcatgat
    22261 gctgtgacag cggaatacgc tgaatgcatc gctcaaaccg tcgaggcggc tgtcagtctc
    22321 ggtatcacgg ctatcgatca ggaaacgata tgccgctaac aagcctgccg ggccgataaa
    22381 cttatcggga ttccaccaga aagacggaca agaggttgaa caacatgcgc agagaataca
    22441 ttcatacagc ccgtcgagtt tttcgcgctg ctctggcatc tgtaaatgct cgcgagctgg
    22501 cggattttgt ccattattca acaggtaagg cttaattttc tcatattgcg catagaattg
    22561 tcccatgtct accaccaaat cgcggatcac cggtaaacct ggcagcgggc gaatcacaat
    22621 cttcttgccc ggctggttga gtgccgaaat cggggtaata caggccagac cattcttgcc
    22681 gttcatgttc agaccgtcgg aaccgcacac accttcacgg caggagcggc ggaacgacag
    22741 gctgggatct ttctctttca gctggataag cgcatccagc agcatcatgt cgcgaccttc
    22801 ttccgcttcc agggggtaat cctgcatacg cggagcatca tcaacatccg ggttatagcg
    22861 ataaattgaa aactcgagtc tcattttcct gtctccgcat tagtaagtac gaatcttcgg
    22921 cgggaatgcc gggcgcagtt tcggttccat gttgacgctt cggcgcgtca tggattccga
    22981 ctctggcaga tacagggagt ggcacagcca gttttcatca tcacgatccg ggaagtcgaa
    23041 gcggctatgt gcgccacggc tttcggtacg gaagttggca gaaacagccg ttgcatacgc
    23101 cgtttccatc aggttatcca gttccaagca ctcaacgcgc tgggtgttga actcgctgga
    23161 agtgtcatcc agacgggcat ttttcagacg ctcgcggatc actttcaact gctcaagccc
    23221 tttcgccatc gcatcacctt cacggaagac cgagaagtta tgctgcatac attcttgcag
    23281 cgctttacga atcgccaccg gatcttcacc gttacgatta ttgttccagc ggtgcagacg
    23341 atccagagat gcttcaacat cagactcgct ggcatcgcgc aatgcgccct gttcggcgat
    23401 agactcttgc agatgcagac ctgccgcacg accaaagacc accaggtcca gcagcgagtt
    23461 gccgcccaga cggttagcgc cgtgtaccga tacacaagcg atttcaccaa cggcaaacag
    23521 ccccggaaca accacatctt cgcctttctc gttcaccgtc agcgcctgac cggtcacttt
    23581 ggtcggaata ccgcccatca tgtagtgaca ggttgggata accggaatcg gctctttcac
    23641 cggatcgacg tgagcaaagg tacgggaaag ctccaggata cccggcagac gggattcgag
    23701 aacttcttta cccaggtgat cgagtttcag tttcgcgtgt ggcccccacg gaccatcaca
    23761 gccgcgacct tcacggattt caatcatgat ggaacgcgcc accacatcac ggcccgccag
    23821 gtctttggcg ttcggcgcat aacgctccat aaagcgttcg ccatgtttgt tcagcagata
    23881 accgccttca ccacggcaac cttcggtgac cagtacgccc gcaccggcaa tgccggtcgg
    23941 gtggaattgc cacatttcca tatcctgcac cggtacgcct gcgcggatag ccatgccgac
    24001 accgtcgcca gtgttaatgt gggcgttagt ggtggactga taaatacgcc ctgccccgcc
    24061 agtcgccagc acggtagcgc gggctttgaa ataaaccact tcaccggttt cgatgcacag
    24121 tgcggtacaa cccaccaccg cgccatcctg gtttttcacc agatccagcg cataccactc
    24181 ggagaaaatg gtggtgtggt ttttcaggtt ctgctgataa agcgtgtgca acagtgcgtg
    24241 accggtacgg tcagccgccg ccgcagtgcg tgccgcctgc tcgccgccga agtttttcga
    24301 ctgaccgcca aacggacgtt gatagatacg accatcatca agacgcgaga acggcaggcc
    24361 catgtgttcg agttccagaa tcgcttccgg cccggtttta cacatatatt caatcgcgtc
    24421 ctggtcaccg atatagtccg acccttttac ggtgtcgtac atatgccatt cccagttatc
    24481 ttcatgggta ttacccagcg caacggtaat gccgccttgc gcagaaacgg tatgggaacg
    24541 ggtcgggaag actttagaga gcagcgcaca ggtctggccg ctctgggaaa tttgcagcgc
    24601 cgcgcgcata cctgcgccac cggcaccaat cacaactgca tcaaattctc tgactggcaa
    24661 tttcatcaca caccccacac cacaacgaat ccataaatca cgtaaaccac cagtgcaacg
    24721 acaatcacca gttgcagcat caggcgcaat gccagcggtt taacgtagtc ggtcaacacc
    24781 tgccacatgc cgatccaggc atgaatcaag atagaaaaca gcgccagcag ggtgaacact
    24841 ttggtgaacg cagaggcgaa gaaaccgatc cagacttcat atgtcagttc gccactggtg
    24901 gcgaaaaaac cgaccatata aatgatgtag agcgtcagga cgatagcggt agcgcgaacg
    24961 aggatgaaat catgtacgcc attgcgtcct aatgcggagg cgttgcttac catacgagga
    25021 ctcctgcgag aagtgaaagc acgacagtaa taacaaagga gattttggcg gagcgtttac
    25081 ccgcttcgaa tgtttcttcc agatagccaa aatccatcat catgtggcga atacctacga
    25141 cgacgtgata cgccagagcg gtaaggatgc cccacatgat aaatttgacg aagaagctgc
    25201 ccataatcgc ggaagcttgc tcgaaacctt caggggaaga gaggctggta cccagaagcc
    25261 acagcaggat gcccactgca acaaaggtga tcacaccgga aacgcgatgg agaatggacg
    25321 ctatcgccgt gatggggaac cggatggtct gtaggtccag attaacaggt ctttgttttt
    25381 tcacatttct tatcatgaat aacgcccaca tgctgttctt attattccct ggggactacg
    25441 ggcacagagg ttaactttct gttacctgga gacgtcggga tttccttcct ccggtctgct
    25501 tgcgggtcag acagcgtcct ttctataact gcgcgtcatg caaaacactg cttccagatg
    25561 cgaaaacgac acgttacaac gctgggtggc tcgggattgc agggtgttcc ggagacctgg
    25621 cggcagtata ggctgttcac aaaatcatta caattaacct acatatagtt tgtcgggttt
    25681 tatcctgaac agtgatccag gtcacgataa caacatttat ttaattttta atcatctaat
    25741 ttgacaatca ttcaacaaag ttgttacaaa cattgccagg aaaagcatat aatgcgtaaa
    25801 agttatgaag tcggtatttc acctaagatt aacttatgta acagtgtgga agtattgacc
    25861 aattcattcg ggacagttat tagtggtaga caagtttaat aattcggatt gctaagtact
    25921 tgattcgcca tttattcgtc atcaatggat cctttacctg caagcgccca gagctctgta
    25981 cccaggtttt cccctctttc acagagcggc gagccaaata acaaacgggt aaagccaggt
    26041 tgatgtgcga aggcaaattt aagttccggc agtcttacgc aataaggcgc taaggagacc
    26101 ttaaatggct gatacaaaag caaaactcac cctcaacggg gacacagctg ttgaactgga
    26161 tgtgctgaaa ggcacgctgg gtcaagatgt tattgatatc cgtactctcg gttcaaaagg
    26221 tgtgttcacc tttgacccag gcttcacttc aaccgcatcc tgcgaatcta aaattacttt
    26281 tattgatggt gatgaaggta ttttgctgca ccgcggtttc ccgatcgatc agctggcgac
    26341 cgattctaac tacctggaag tttgttacat cctgctgaat ggtgaaaaac cgactcagga
    26401 acagtatgac gaatttaaaa ctacggtgac ccgtcatacc atgatccacg agcagattac
    26461 ccgtctgttc catgctttcc gtcgcgactc gcatccaatg gcagtcatgt gtggtattac
    26521 cggcgcgctg gcggcgttct atcacgactc gctggatgtt aacaatcctc gtcaccgtga
    26581 aattgccgcg ttccgcctgc tgtcgaaaat gccgaccatg gccgcgatgt gttacaagta
    26641 ttccattggt cagccatttg tttatccgcg caacgatctc tcctatgccg gtaacttcct
    26701 gaatatgatg ttctccacgc cgtgcgaacc gtatgaagtt aatccgattc tggaacgtgc
    26761 tatggaccgt attctgatcc tgcacgctga ccatgaacag aacgcctcta cctccaccgt
    26821 gcgtaccgct ggctcttcgg gtgcgaaccc gtttgcctgt atcgcagcag gtattgcttc
    26881 actgtgggga cctgcgcacg gtggtgctaa cgaagcggcg ctgaaaatgc tggaagaaat
    26941 tagctccgtt aaacacattc cggaatttgt tcgtcgtgcg aaagataaaa atgattcttt
    27001 ccgcctgatg ggcttcggtc accgcgtgta caaaaattac gacccgcgcg ccaccgtaat
    27061 gcgtgaaacc tgccatgaag ttctgaaaga gctgggcacc aaagatgacc tgctggaagt
    27121 ggctatggag ctggaaaaca tcgcgctgaa cgacccgtac tttatcgaga agaaactgta
    27181 cccgaacgtc gatttctact ctggtatcat cctgaaagcg atgggtattc cgtcttccat
    27241 gttcaccgtc attttcgcaa tggcacgtac cgttggctgg atcgcccact ggagcgaaat
    27301 gcacagtgac ggtatgaaga ttgcccgtcc gcgtcagctg tatacaggat atgaaaaacg
    27361 cgactttaaa agcgatatca agcgttaatg gttgattgct aagttgtaaa tattttaacc
    27421 cgccgttcaa atggcgggtt aatttttata tgcccaaaca caaaaaattg taaaaataaa
    27481 atccactatc ggacattcat atattctaaa aaaaatatat cggtataaat tataaacaaa
    27541 tcgatgattt ttaatcatct atcttcctct cagttacatg aataaaccac caaatatagc
    27601 gcacccctat aatttccaat actttattca tcaaacattt aattcatacc atcaattaat
    27661 tacttttaca tttaataaaa ttaataatat ttttaagaaa aactaaaaac attcgacaat
    27721 caaataagat attcttgcac cgatatactt ttttcttttc tgttaaagga ctcagttatg
    27781 tttaaaggac aaaaaacatt ggccgcactg gccgtatctc tgctgttcac agcacctgtt
    27841 tatgcggctg atgaaggatc cggtgaaatt cactttaaag gtgaagttat tgaagcaccg
    27901 tgtgaaatac atcaggatga tattgataaa gaggttgaac tcggtcaggt gaccaccagc
    27961 cacattaatc agtcacatca cagcgatgcc gttgctgtcg acctgcgctt agtcaactgt
    28021 gatctggaaa actccagcaa cggttccggt ggcaagattt ctaaagttgc agtcaccttt
    28081 gatagctcag cgaaaaccac cggcgcagat ccgattctca acaacaccag cacgggtgaa
    28141 gccaccggcg tcggcgtacg tttaatgaat aaagatcaaa gcaatatcgt tttaggcacc
    28201 gctactccag atatcgacct ggctccgacc tccagcgaac aaacgctgaa tttctttgcc
    28261 tggatggaac aaattgatca ggctacacct gtaacgccag gcgcagttac ggcgaatgca
    28321 acctacgtgc tggattataa ataagtatta ctttggcggc gctatcgccg cctttcataa
    28381 aatatttatc aggtatggac accgtgaata tttatcgact ctcttttgta tcctgcctgg
    28441 tcgtggcgat gccttgcgca ttggcggtcg aattcaacct taatgttctc gataaatcga
    28501 tgcgcgaccg cattgatatt tcattattaa aggaaaaagg agtcattgct cccggtgagt
    28561 attttgttag cgttgcggta aataataacc aaatcagtaa cgggcaaaag attaactggc
    28621 acaaaaatga cgataaaacc attccgtgca tcaatgattt actggtcgat aaatttggct
    28681 taaaacctga agtccgtcag tcgttaccat tgataaatca gtgcgtcgat tttagctccc
    28741 gacctgaaat gctcttcaat ttcgatcaag ccaatcagca actgaatatt agtattccgc
    28801 aagcctggct ggcgtggcac tcagaaaact ggaccccacc ctccacatgg aaagaaggtg
    28861 tcgccggtat cctgatggat tacaacttgt ttgccagcag ctaccgccca caggacggca
    28921 gcagcagcac taacctgaac gcctacggta ccaccggaat taacgccggg gcatggcgct
    28981 tacgtagtga ttatcagttg aatcagactg atagcgatga taaccatgaa cagtcaggcg
    29041 gaatatcgcg cacctatctt tttcgtccat taccgcaatt aggctctaaa ttaaccctcg
    29101 gcgaaacgga ttttagttcc aatattttcg acggtttttc ttataccggc gcggcactgg
    29161 caagtgacga gcgaatgttg ccatgggaac tacgcggcta cgccccacaa attagcggta
    29221 ttgcacagac caatgccacg gtgacgatca gtcaatcagg ccgcgtcatt taccagaaaa
    29281 aagtcccacc aggcccattt atcattgacg accttaatca gtctgttcag ggcacactgg
    29341 atgtcaaagt gacggaagaa gatggtcggg tgaacaattt ccaggtttcg gcagcatcga
    29401 cgcccttcct gactcgtcag ggacaggttc gctataaact ggccgcgggt cagccacggc
    29461 cctccatgtc acatcaaact gaaaatgaaa ccttttttag caatgaagtt tcctggggga
    29521 tgctgtcaaa cacctcgctg tacggcggcc tgctgctttc tggtgatgac taccattctg
    29581 ccgcaatggg tattgggcaa aatatgctgt ggcttggtgc gctgtcgttt gatgtcacgt
    29641 gggccagtag ccattttgat actcagcagg acgagcgggg cttaagctac cgttttaatt
    29701 acagcaaaca agtggatgcc actaacagca cgatttccct cgccgcttat cgtttctccg
    29761 atcgtcattt tcacagctac gccaactatc tggatcacaa atacaacgac agcgatgcgc
    29821 aggacgaaaa acagacgatc agcttattgt gggccaaccg attaccccac taaacctcaa
    29881 tctttacgcc aacctgctac atcaaacctg gtggaatgca gacgcctcca cgaccgccaa
    29941 catcacggca ggttttaatg ttgatattgg tgactggaga gatatctcta tttcgacgtc
    30001 attcaataca acccattacg aagataaaga tcacgacaac cagatttacc tgtcgatttc
    30061 gctccccttc ggtaacggtg gtcgggttgg ctatgacatg caaaacagta gccacagcac
    30121 cacacaccgc atgtcgtgga acgatacgct ggatgaacgt aatagctggg gcatgtctgc
    30181 cggactgcaa tccgaccgtc ctgacaatgg agcccaggtg agcggtaact atcagcacct
    30241 gagttcagcg ggtgagtggg atatttctgg tacctatgcc gccaatgatt acagttccgt
    30301 cagcagcagc tggagcggtt ctttcaccgc aacccaatat ggtgcagcgt ttcatcgccg
    30361 cagctccacc aatgaaccac gcctgatggt cagcaccgat ggcgtggcag atattccggt
    30421 tcagggcaat ctcgactaca ccaaccattt tggcattgcg gtggtgccgt tgatttccag
    30481 ttaccagcct tccaccgtgg cggtgaacat gaatgactta cccgacggcg taacagttgc
    30541 agaaaacgtt atcaaagaaa cgtggattga aggcgcgata ggttacaaat cactggcttc
    30601 ccgttccggt aaagacgtta acgtcatcat tcgcaacgcc agcggtcagt ttcctcccct
    30661 cggagcggat atccgccagg atgacagcgg cattagcgtg gggatggttg gcgaggaagg
    30721 acatgcctgg ttaagcggag tcgctgaaaa tcaaaagttt accgtggtct ggggtgatag
    30781 ccagcattgc tcgctccatc ttcctgaaca tatggaagac accgcaaatc gcctgatttt
    30841 accttgtcat taattaagga aatgaaaatg acatttatga aaggactacc ccttttgttg
    30901 ttggttgcca gtctgtgcag ccacgctgca ctacaacccg atcgcactcg tattgtgttt
    30961 aacgctaatg ataaagctac cagtctgcgg gtggataatc gtagcgataa actcccctac
    31021 ctcgcttatt cctggctcga aaatgaaaaa ggggagaaaa gcgatgatct tctggtcgcc
    31081 ctgccgccga tacaacgtct ggaaccgaaa gcaacaacgc aggtacggat agtaaagcaa
    31141 gcctcgacca caaaattacc gggcgatcgc gaaacgctat ttttctacaa tatgcgtgaa
    31201 attcctcccg ccccggaaaa aaacagcgac catgccgttc tgcaagtcgc cattcagagt
    31261 cgtatcaagg tgttctggcg accggctgca ttacgcaaga aagcaggaga aaaggtcgaa
    31321 ctgcagttac aggtcagcca gcagggcaac caactgacac tgaaaaatcc taccgcgtat
    31381 tatctgacca ttgcctacct gggacgaaat gaaaaaggcg ttctccccgg cttcaaaacc
    31441 gtgatggttg cgccttttag tacggtcaac acaaacaccg gaaattatag cggcagccag
    31501 ttttatctcg gttatatgga tgattacggc gcgttacgca tgacgacgct caactgcagc
    31561 ggacaatgcc atttacaagc cgtggaggcg aagaaatgag tgctggcaag ggagtgttgc
    31621 tcgtcatctg tctgttattt ctgccgttga agtctgcgct tgccctgaac tgctactttg
    31681 gttcatccgg tggctcggta gaaaaatcag aagcgattca accttttgct gtaccaggca
    31741 atgccaaact cggcgataag atttgggaat ctgacgatat taaaattccc gtctattgtg
    31801 acaacaatac caacggcaat tttgaaagtg agcatgttta tgcctgggta aacccctacc
    31861 cgggggtgca ggatcgctat tatcaactcg gcgtaactta caatggcgtt gattatgacg
    31921 ccaaccaggg aaaaagccgc atcgacacta accagtgtat tgacagtaag aacattgaca
    31981 tttatacccc tgagcagatt atcgcgatgg gatggcagaa caaaatttgc tcgggtgatc
    32041 ccgctaatat tcacatgtcg cgtacctttc tcgctcgcat gcggttatac gtcaaaatac
    32101 gagaaatgcc gccgcatgat tatcaaagta cgcttagcga ctatatcgtc gtgcaatttg
    32161 acggtgccgg tagcgttaac gaagacccta ctgcccaaaa cctgaaatat catattactg
    32221 gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt ttccatttca ccggaaacac
    32281 aggtgattga ttttggtaaa tttaatttgc tggatattcg tcgccacaca atgtcgaaaa
    32341 cattcagtat taagacgaca aaatcacaaa atgaccaatg caccgacgga tttaaagtca
    32401 gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga taaagcattg ttgattggta
    32461 atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc ttataccttc aataaatatg
    32521 ctgaatacgc cgatttcacc agcgacatgt tggtctacga aaaaacctat acagctgaac
    32581 tttcgtccat tgcaggtacc cccatcgagg ctgggccctt cgatacagtg gtgcttttta
    32641 aaattaacta taactgattt gccatgatgc cctttgcagc cggaaagggc atctctttca
    32701 gaaaattgct gattttcccc ttttccttga ttgctgttat tttttcctgg cccggctttc
    32761 tttatgctgt atcgatacac caaagagaaa ataatgaggg agcgaaggat gccagttttg
    32821 cagtggggaa tgttatgtgt gctatcactt ctcctttcta ttggtttcct cgcggtacat
    32881 ctcccggcgg cgctattact tgggccgatg atcgccggga tcatcttcag tatgcgcgga
    32941 attactctgc aacttccccg ctccgctttt ctcgccgcgc aggccattct tggctgcatg
    33001 attgcgcaaa acctcactgg ttcgattctc accacgctgg ccgccaactg gccgatcgtg
    33061 cttgctattt tactggtgac gctgctttcc agcgccatcg tgggttggtt attggtgcgc
    33121 tatagctcac tgcctggaaa taccggtgcc tggggttctt ctccaggcgg cgcagcggca
    33181 atggtcgcta tggcgcaaga ttacggcgca gatattcgcc tggttgcatt tatgcaatat
    33241 ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca cccgcatgat gctgggtgat
    33301 aacgctgaag cggttaacca gcaaatcgtc tggttcccgc cagtgagcat taatctcctg
    33361 cttacgattt tgctcgcagt cgttgccggt acggcgggat gcatgctgcg tcttccttcc
    33421 ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc agtctggtca gctcatcacc
    33481 atcgaactac cggaatggct gctggcgatg gcgtatatgg caattggctg gcggattggt
    33541 cttggtttcg ataaacaaat cttactgcgg gcactacgcc cgctaccgca aatcctgctg
    33601 tcgatttttg ctctgctggc tatttgtgcg ggtatggcgt gggggctgac ccggtttatg
    33661 cacattgatt ttatgactgc ctacctcgcc accagtcccg gcgggcttga taccgtggcg
    33721 gtcatcgccg cagggagcaa tgccgatatg gcgcttatta tggcgatgca aaccctgcgc
    33781 ctgttcagta ttttgctgac ggggccagcc attgcgcggt ttatttcaac ctatgcgccg
    33841 aagcggtcgg cctagtgctg gcagccaggg caccagtaaa acgggcgaga tgacagcgtg
    33901 gttttctcaa tgatgctgcc acagcgttcg cacggttcgc catcacgatg aaaaacctta
    33961 aagcgaaaca gcgccccatg atgcttattc tcatccacca gccccctcgt agcgtaggaa
    34021 aatcgaggaa tatccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc attgagatct
    34081 ttcgctttat gatttccagt caacccaacc tgccagagga tctccacccg caaataattg
    34141 ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg caaactgacg gttacgaaag
    34201 cgcggtgaca ataaccgttc ctttaccacc tccggcgtca gattcggatc cagcacatcg
    34261 ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt ccggcctcaa catctcaata
    34321 tccgaggcgc tataaagcag aatggttttg tcagccgttt gcagttttac ccgcaatact
    34381 cgcgtggtct gcggctcttc gccggtatca accacgcgcc agacgccgta aagctgatta
    34441 tggctgtaga gcgttaagtc gttggaaaaa tgagttaaca acgccttacc acgcgtttcc
    34501 acatgggtaa cgtgttgacc gataagttgt gattgataag tttttaactg cgggaaggca
    34561 aaccagacat cagttagtgg tttgcctttg atcgccgcct ccaggttatc cgctgcacgg
    34621 cggatctccg ggccttcagg catggtgata tccttttttc attctttatt aatcggggtt
    34681 atgctgcgac aacaatctcc ttttcggcaa atgtagagcg tagtcggcgg gcgaacgcca
    34741 gtgcgtgctc gccgtcgcca tgcaggcaga ctgtttgcgc cgtgaccgtt gcccattcgc
    34801 cggtgatact tttgactctg ccgtgttgca ccatctctag cgtttgcgcc aacgcctgtt
    34861 cttcgttttc aatcaacgcg cctcgctgac ttcgcggcac cagcgagccg tcagcctgat
    34921 aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa
    34981 tcagctcgct tcctgccagc ccaacgagaa tcaatgccgg atcgcaagcg tataccgctc
    35041 tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg gttgtacaac atgccgtgcg
    35101 gtttgacatg acgcattacg ccgccttgcg cacgggcaat cgttgccagc gcgccaattt
    35161 gatacagcgt ctgggcgtaa acggtttctg gcggcagctg catggcgctg cgaccaaaat
    35221 tttccctgtc gggaaaactc gggtgagcgc caatcgcgac accatttttt attgcttcac
    35281 gcacgcaagc ctgcatgatt tgcgcatcgc ctgcatgaaa tccacaggca atattggcag
    35341 aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca
    35401 ggtcaatttt cattgtgcag ccgccacact aattgttcga aataacgttg ctgatcttgc
    35461 cgcgctttca gtgcctcttc cagtgaacac tggacaaaat gaatcggctg accgagcgga
    35521 atttgcgcca gatggtacat atcagcctca atgatacagg caatacgcgg gtaaccatcg
    35581 gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt tatgtggcac ctgcaccacg
    35641 cccggtaaca aaccgtgaga taacagttcg cgatcggtgg tgcgttttaa aatttgcccc
    35701 tgtaagcgat agcccatgcg gttactttgc gagctaagct gccagggcga acgccagaat
    35761 gcatcctgcg aggcgcgatc gaactcatga tattccggcc ccggcaaggc gcgaatgcgg
    35821 ttgccccaca gcagctgttt aacgccctgc gcttccataa aatcacgctt cgatttgcca
    35881 atcgggagtc ggtcaccatc cttcagtaaa cggccttcca gcccgccaat ccccactttg
    35941 agatcggtgc tgcatgaccc cattaccggc ggaacatcaa taccacccgc gaccgccaga
    36001 taactgcgca tcccgtgctg cgggcgttta agcgttaaac gctggcctgc tttcatcggc
    36061 aatcgccagc cggtccagac ggcattatca tccagccgcg cttcgcaacc ggcacccgtc
    36121 agagcaaacc acccatcagt ttcgaactca accgttaact gaccgagcgt gatctccagc
    36181 gcgggggcat tggcgtcatt acccaccagc aggttagcaa tgcgtaacgc aggcatatcc
    36241 agtgcgccgc agtggctgat tcccgactgg cgaaaaccgt gacgaccgcc atcctgcaca
    36301 gtggtataca tgcccgcacg aataatcttc agcatactcc ctccttctgc ggtacaaagc
    36361 gcacgctgtc tcccggacgt aataagatgg gttcgtcacg cgccggatca aacaggctga
    36421 gtgaggtatg accaatcaac tgccagccac ccggcgttgc cagcggataa acaccagtct
    36481 gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa gcgcggttca gcgcgccgtg
    36541 gcgtgtgtag ttgttccggc aaactcccga gatacgggaa gcccggttga aaacctaaaa
    36601 accagaccac gtattccacg gaggagtgca attcaacaac ctgtttttcg ctaaacccgc
    36661 aatgcgccgc gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc accggaattt
    36721 caataaagcg agactcaggt tccagggcct cgctctcctc ccaccagcgt tgcaaacgct
    36781 ctatggcatc cagcgccagc gactcaggat tacgcaaaat caccgtgata ttgttcatgc
    36841 cgggaatggc ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt cgccagatcc
    36901 gtttctggct agccagcgtc accggcggtt ccagttccag cactaccgcc gtttcaccta
    36961 tcagataaca acgcgctcgt tgcactttcg atccctctga ttattcgtta tgcaggatta
    37021 ggaatatcaa taaaggtcac atcaagatcg gtattttcat tcagccactc gctcaatgcg
    37081 cgaataccac cacgttcagt ggcatggtga cctgcagcat aaaaatgcaa cccctgctcg
    37141 cgggctgaat gaatggtctg ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa
    37201 cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc accaggcgac gcgctggacc
    37261 acctcaggtc cggtatcgcc acaccataac ggcttacgtc ccagacgcgc ttcaatccag
    37321 gaggctagtt ccagtcccgg cacaggcatg gtcagctctc cccacggcac caacggctca
    37381 atttcgccca tgaccgtgat ccccagtaac gccgccagct gtgcgttatt gcccagctca
    37441 ggatgtacgt caagcggcaa atgccagcca tacaggttga tatcattcgc cagcaacgtt
    37501 tttaaacggt tacgcttcat tccgcgaatg accggagact ctcctttcca gaagtagcca
    37561 tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa agcctggctg
    37621 gcggtgacac cggtaacaat tttttgcacc gtctctttgc cttctacctg caaaccattc
    37681 ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt taatcagttg ttccagttcg
    37741 gtgtttttca tcttcgcgcc ttccgtattc tgatagctac accgttaaca tagcgttacc
    37801 tcgcctgttc gtcgatagcc aatcttttga catattaaga aacaaacccg acgccaaaag
    37861 acgcaaattg tttatatcat cacgcttcag ctaatgtata tttatgcaat cagacagcat
    37921 attgcataaa taatcagatc tgtatcaaag ttttaacttt aaggaaacac aagcacgccg
    37981 cgcaactatt acgtgacgtt gcagtcaatt gtggaattat tatgaataaa cacgcatcac
    38041 aaccgcgcac tatttactat gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg
    38101 gcatgcgtgc cctgctgatt ctctatctca ccaatcaact aaaatacaac gatactcacg
    38161 cctacgagtt atttagcgcc tactgttcgc tggtgtatgt cacgccaatc ctcggtggct
    38221 ttttggcgga taaagttctc ggcaatcgca tggcggtgat gctgggggcg ttgttgatgg
    38281 cgatcggtca tgtggtgctg ggtgccagtg agatccatcc gtcattcctc tatctgtccc
    38341 tggcgattat cgtttgtggc tatggtctgt ttaaatccaa tgtcagttgc ctgctcggcg
    38401 agctgtatga gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg tatgcagcgg
    38461 gtaacgtggg gtctattatc gcacctatcg cctgtggtta cgcccaggaa gagtacagct
    38521 gggcgatggg ctttggcctg gcggcggtgg gtatgatcgc gggtctggtc attttcttat
    38581 gtggcaatcg tcatttcaca catacccgcg gcgttaataa aaaagtactg cgtgcaacaa
    38641 actttctcct gcctaactgg ggatggctgc tggttctgct ggtggcaacg cctgcgctga
    38701 ttaccgtact gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg actattattg
    38761 gcctgggtgt actggcaaaa atttatcgca aagcagaaaa ccagaaacag cgtaaggagc
    38821 tggggctgat tgtgacgctc accttcttca gtatgttgtt ctgggccttc gcacaacagg
    38881 gcggcagctc aattagcctt tatatcgacc gcttcgttaa ccgcgatatg tttggttata
    38941 ccgttccgac tgctatgttc cagtcgatta atgccttcgc agttatgcta tgcggtgtgt
    39001 tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg taccgtgcgc atctggggaa
    39061 aatttgctct tggccttggc ctgatgagcg ccggattctg cattctgacc ttaagcgccc
    39121 gctggtccgc aatgtatggt cactcttctc tgccactgat ggtgttaggc ctcgcggtga
    39181 tgggctttgc ggaactgttt atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa
    39241 ttccgggcgt gaccggcgta ttaaccggca tctacatgct gctttctggc gcgatcgcga
    39301 actatctggc gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat gcttccgggg
    39361 cgatcaacta ctccatcaat gcatatattg aagtatttga tcaaattacc tggggcgcac
    39421 tggcgtgtgt aggactggta ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc
    39481 gcgcgctggc gctggagtca taataggcca gcaccgatgc ctgatgcgac gcttgacg
//
LOCUS       DAJTYZ010000022        39401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000022 DAJTYZ010000000
VERSION     DAJTYZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.022"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..256
                     /locus_tag="OO596_001127"
     CDS             <1..256
                     /locus_tag="OO596_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6717036.1"
                     /translation="EAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKK
                     AQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            350..748
                     /gene="fadM"
                     /locus_tag="OO596_001128"
     CDS             350..748
                     /gene="fadM"
                     /locus_tag="OO596_001128"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6717037.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(800..1495)
                     /gene="queC"
                     /locus_tag="OO596_001129"
     CDS             complement(800..1495)
                     /gene="queC"
                     /locus_tag="OO596_001129"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6717038.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1560..3260)
                     /locus_tag="OO596_001130"
     CDS             complement(1560..3260)
                     /locus_tag="OO596_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR6717039.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3360..4178
                     /gene="cof"
                     /locus_tag="OO596_001131"
     CDS             3360..4178
                     /gene="cof"
                     /locus_tag="OO596_001131"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6717040.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4330..4788
                     /gene="decR"
                     /locus_tag="OO596_001132"
     CDS             4330..4788
                     /gene="decR"
                     /locus_tag="OO596_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6717041.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4818..6590
                     /locus_tag="OO596_001133"
     CDS             4818..6590
                     /locus_tag="OO596_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6717042.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6583..8364
                     /locus_tag="OO596_001134"
     CDS             6583..8364
                     /locus_tag="OO596_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6717043.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8545..8883
                     /gene="glnK"
                     /locus_tag="OO596_001135"
     CDS             8545..8883
                     /gene="glnK"
                     /locus_tag="OO596_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6717044.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8913..10199
                     /gene="amtB"
                     /locus_tag="OO596_001136"
     CDS             8913..10199
                     /gene="amtB"
                     /locus_tag="OO596_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6717045.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10248..11108)
                     /gene="tesB"
                     /locus_tag="OO596_001137"
     CDS             complement(10248..11108)
                     /gene="tesB"
                     /locus_tag="OO596_001137"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6717046.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11326..11898
                     /locus_tag="OO596_001138"
     CDS             11326..11898
                     /locus_tag="OO596_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6717047.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11929..12240)
                     /locus_tag="OO596_001139"
     CDS             complement(11929..12240)
                     /locus_tag="OO596_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6717048.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12403..12499
                     /gene="ffs"
                     /locus_tag="OO596_001140"
     ncRNA           12403..12499
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO596_001140"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12619..12972
                     /locus_tag="OO596_001141"
     CDS             12619..12972
                     /locus_tag="OO596_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6717049.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13014..14562)
                     /gene="pdeB"
                     /locus_tag="OO596_001142"
                     /pseudo
     CDS             complement(13014..14562)
                     /gene="pdeB"
                     /locus_tag="OO596_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14726..15196)
                     /locus_tag="OO596_001143"
     CDS             complement(14726..15196)
                     /locus_tag="OO596_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6717050.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15312..15863)
                     /gene="maa"
                     /locus_tag="OO596_001144"
     CDS             complement(15312..15863)
                     /gene="maa"
                     /locus_tag="OO596_001144"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6717051.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16034..16252)
                     /gene="hha"
                     /locus_tag="OO596_001145"
     CDS             complement(16034..16252)
                     /gene="hha"
                     /locus_tag="OO596_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6717052.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16278..16652)
                     /gene="tomB"
                     /locus_tag="OO596_001146"
     CDS             complement(16278..16652)
                     /gene="tomB"
                     /locus_tag="OO596_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6717053.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17198..20347)
                     /locus_tag="OO596_001147"
     CDS             complement(17198..20347)
                     /locus_tag="OO596_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6717054.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20370..21563)
                     /gene="acrA"
                     /locus_tag="OO596_001148"
     CDS             complement(20370..21563)
                     /gene="acrA"
                     /locus_tag="OO596_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6717055.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21705..22352
                     /gene="acrR"
                     /locus_tag="OO596_001149"
     CDS             21705..22352
                     /gene="acrR"
                     /locus_tag="OO596_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6717056.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22480..25842
                     /gene="mscK"
                     /locus_tag="OO596_001150"
     CDS             22480..25842
                     /gene="mscK"
                     /locus_tag="OO596_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6717057.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26054..26215)
                     /gene="rsmS"
                     /locus_tag="OO596_001151"
     CDS             complement(26054..26215)
                     /gene="rsmS"
                     /locus_tag="OO596_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6717058.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26229..26756)
                     /gene="priC"
                     /locus_tag="OO596_001152"
     CDS             complement(26229..26756)
                     /gene="priC"
                     /locus_tag="OO596_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6717059.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26826..27203
                     /locus_tag="OO596_001153"
     CDS             26826..27203
                     /locus_tag="OO596_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6717060.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27356..27907
                     /gene="apt"
                     /locus_tag="OO596_001154"
     CDS             27356..27907
                     /gene="apt"
                     /locus_tag="OO596_001154"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6717061.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28036..29961
                     /gene="dnaX"
                     /locus_tag="OO596_001155"
     CDS             28036..29961
                     /gene="dnaX"
                     /locus_tag="OO596_001155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6717062.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29294..29358
                     /gene="dnaX"
                     /locus_tag="OO596_001155"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30014..30343
                     /locus_tag="OO596_001156"
     CDS             30014..30343
                     /locus_tag="OO596_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6717063.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30343..30948
                     /gene="recR"
                     /locus_tag="OO596_001157"
     CDS             30343..30948
                     /gene="recR"
                     /locus_tag="OO596_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6717064.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31058..32932
                     /gene="htpG"
                     /locus_tag="OO596_001158"
     CDS             31058..32932
                     /gene="htpG"
                     /locus_tag="OO596_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6717065.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33113..33757
                     /gene="adk"
                     /locus_tag="OO596_001159"
     CDS             33113..33757
                     /gene="adk"
                     /locus_tag="OO596_001159"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6717066.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33889..34851
                     /gene="hemH"
                     /locus_tag="OO596_001160"
     CDS             33889..34851
                     /gene="hemH"
                     /locus_tag="OO596_001160"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6717067.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34848..35807)
                     /gene="aes"
                     /locus_tag="OO596_001161"
     CDS             complement(34848..35807)
                     /gene="aes"
                     /locus_tag="OO596_001161"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR6717068.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35959..37263
                     /gene="gsk"
                     /locus_tag="OO596_001162"
     CDS             35959..37263
                     /gene="gsk"
                     /locus_tag="OO596_001162"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6717069.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37396..39072)
                     /locus_tag="OO596_001163"
     CDS             complement(37396..39072)
                     /locus_tag="OO596_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6717070.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9522 a        10047 c        10417 g         9415 t
ORIGIN      
        1 ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg acgaagaagg
       61 cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga tgaatggcgt
      121 tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc cgtttaaaac
      181 ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac gtaatctggc
      241 ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg aagaggtcat
      301 accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta tgcaaacaca
      361 aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg cccgctacct
      421 tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt ttcagtggat
      481 gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc gtcgcccagc
      541 ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg gtaaaagcgg
      601 catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg atgcgcttat
      661 tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag gggaattgcg
      721 cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct caagccgcat
      781 ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct gccatcaccg
      841 tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat gccgcacaat
      901 gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta cggactaaat
      961 ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc cacatcagcg
     1021 gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt agtgctttca
     1081 caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg ccggtaatta
     1141 cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac aaaatattac
     1201 gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca ggcaccggaa
     1261 tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca tcaagaacct
     1321 tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt tctgcgcgat
     1381 gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt aatgcctgca
     1441 ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt ttcatagata
     1501 atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg accagctatt
     1561 cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg cggtgtcagt
     1621 cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa cagcggcgtc
     1681 agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa tcggttttcc
     1741 tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata tgctggagcg
     1801 tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt atattccggt
     1861 gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag ggtcgtgtcg
     1921 tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc tgccagtgtc
     1981 gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac cagcgtcaag
     2041 gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca gcctggcagt
     2101 aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa taaaccggat
     2161 tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg acttttgcgc
     2221 aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac ccgttgcagc
     2281 tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc caaatctttt
     2341 tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg acgcaaatgg
     2401 taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa tcgaaaagga
     2461 ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct cgccagccgg
     2521 tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg atgggtgact
     2581 tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa cagcatcaat
     2641 aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg tagggttgaa
     2701 cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg cgctaaatcg
     2761 ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat ctgcccggcg
     2821 agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag cgggcgatag
     2881 tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa cggctgtaac
     2941 agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg ctgctttccg
     3001 gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac cagaaagcgt
     3061 aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc cgcctcctgt
     3121 gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc ggccagttcg
     3181 ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg atactggtta
     3241 agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt cactattact
     3301 tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg gagaaagaaa
     3361 tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac catcatttag
     3421 gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc ctcacttttg
     3481 ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg ctggatgcat
     3541 atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt ttacatcgtg
     3601 atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat acccgagcca
     3661 gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct gcgttgttgc
     3721 aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa atgccactcg
     3781 gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc ttgcagatcc
     3841 agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca gattgcctcg
     3901 aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc caacatttag
     3961 gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc gaaatgttag
     4021 gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc gcggagctcc
     4081 cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat ttgacgcact
     4141 ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc cagcaccggg
     4201 caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt taaaccgcac
     4261 agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa aattctccgt
     4321 ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca gcaggattgc
     4381 accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc ttgctggaag
     4441 cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct gctggatccg
     4501 gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca tcacagcagc
     4561 gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct ggggttctgg
     4621 cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat gaaacgctac
     4681 gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt cacttccagc
     4741 ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat atccagaatc
     4801 aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc cgtcgggaat
     4861 ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg caactggttc
     4921 cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt actaccgggc
     4981 tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat ctcctgcgtt
     5041 atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa ctgcgtgaag
     5101 attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat cgcacgggtg
     5161 atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc ggagaagggg
     5221 tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg atgtctacgc
     5281 aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg gcgatcatga
     5341 ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg gcgttttcca
     5401 gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa gcctttggtc
     5461 tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc aaaaaaaaca
     5521 tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg attggtatgg
     5581 cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt ttaacgctgg
     5641 gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg ctggcgctgg
     5701 catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt cgtgcgatgc
     5761 tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg cgtggcgaac
     5821 ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg ctggaaaacg
     5881 tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact ggttccggca
     5941 aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg gatattcgct
     6001 ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc ctggcggtgg
     6061 tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg ctgggttgcc
     6121 cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta catgacgata
     6181 ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg atgctttccg
     6241 gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg gaaatcctca
     6301 tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc ctgcataacc
     6361 tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt tcggcactga
     6421 cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt ggcaatcatg
     6481 atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa caactggagg
     6541 cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag ttttagccaa
     6601 ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa accgctgggg
     6661 attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc gctgcttatc
     6721 agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt ggttgcaggg
     6781 ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta cgcgcagtcg
     6841 ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga cgtgatggat
     6901 gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca ggtgatttcc
     6961 cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt ggcaactgtc
     7021 ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct cgactggcga
     7081 atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt gatatatcaa
     7141 cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat caacgacggc
     7201 tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca ggcgcgattt
     7261 ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca aaccctgcgc
     7321 ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat tctttgtgga
     7381 ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt gctgtatgcc
     7441 tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac gcaacaggcg
     7501 atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga cggaccgcgc
     7561 cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt cgataacgtg
     7621 tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc tgtgccttcg
     7681 agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct cgccagttta
     7741 ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg tccattaagt
     7801 tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga tccggtggtg
     7861 ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga agaacgcgtc
     7921 tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag cgacggtatt
     7981 tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca actgctggca
     8041 ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc aaccgccagc
     8101 attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg tgaacatacc
     8161 acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac cattctggtg
     8221 cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc tgcccaggga
     8281 cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc cagcgtgcgt
     8341 gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc aaaaatgtaa
     8401 cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc ctgctctctt
     8461 tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc agaaccatta
     8521 ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa accattcaag
     8581 ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac cgtcaccgaa
     8641 gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc ggaatacagc
     8701 gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca actcgatgaa
     8761 gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg caaaatcttc
     8821 gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga agcggcgctg
     8881 taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata aaaactgggc
     8941 ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg gccgataaag
     9001 ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact attccgggga
     9061 ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg ctgacgcagg
     9121 tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac tcgctggcgt
     9181 ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa aacatcgaac
     9241 tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag ggctcgtttg
     9301 cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc ttctcagctg
     9361 tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg catatggtgt
     9421 ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc acggtggtgc
     9481 atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa cgcgtgggct
     9541 tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt actgccattc
     9601 tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat gaaatcgcgg
     9661 cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc tggatcttcg
     9721 gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc gcgattgccg
     9781 gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg ttgattatcg
     9841 gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc ttgctgcggg
     9901 tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc ggctgtatca
     9961 tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa ggtgtgacga
    10021 tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc tggtccggtg
    10081 tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt gtgccggaag
    10141 aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat aacgcgtaac
    10201 aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta attgtgatta
    10261 cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt ataaaactca
    10321 ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata cagcagccat
    10381 tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc aatctgaatc
    10441 cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa gttaagatca
    10501 gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct accatttgcg
    10561 cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt atgaaactcc
    10621 accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac tggcggcagc
    10681 aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg cgctggcgcg
    10741 gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga ggccgtcata
    10801 taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa gctgttaccg
    10861 tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg gcgaagaaag
    10921 tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc agcatacaag
    10981 gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc actctggccg
    11041 cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt ttttagcgcc
    11101 tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga gttgagtata
    11161 acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct aattacaggc
    11221 agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc actcttccgg
    11281 cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa actcgtgcac
    11341 atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa aagcgcggat
    11401 attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca acctgctatc
    11461 cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact gccgcctgat
    11521 gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc aaaagtgctg
    11581 gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt tctgcctttt
    11641 aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat taccgtgaat
    11701 gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg cggaactaaa
    11761 gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc cagcggtggt
    11821 gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc ggcagttcca
    11881 gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt agtagttcca
    11941 gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt ctgccagtaa
    12001 tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt ggcgattaac
    12061 cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac cgcccacctg
    12121 gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg tgacatagcc
    12181 ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt ctttttccat
    12241 cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt gaagtcgcat
    12301 tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc agcagtgata
    12361 atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt ctcccgcaac
    12421 gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg acgcgtgtgc
    12481 cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg tcaaaaaatc
    12541 ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc tgcgcagtac
    12601 gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt tcctgccgat
    12661 aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa agagtttggc
    12721 gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt gaccgatttt
    12781 cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat tgaataccct
    12841 tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg gatgaaagag
    12901 ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt cgagtcaatc
    12961 atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa aattcacaaa
    13021 tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta gagccagccc
    13081 tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt ttcgattccc
    13141 tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg cgtgacattt
    13201 ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac atccagatcc
    13261 tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat ttcatggccc
    13321 gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa ctcgcgttca
    13381 gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt cacgcagcaa
    13441 ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa tatgctgctg
    13501 tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa tcagtagcgt
    13561 caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg gtgacaacca
    13621 actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt tgccattggc
    13681 taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag catcctgcag
    13741 ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg ctgccagcag
    13801 gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc ctttctgcaa
    13861 cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg gcaggatatc
    13921 gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc tggtaataat
    13981 tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc caataatggc
    14041 ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg ggtcgatcat
    14101 gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta aatcgttatg
    14161 cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg ggaaggtatc
    14221 gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga tataaaccac
    14281 ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat gggcttcgct
    14341 acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt gtgtcgccac
    14401 cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa taaacgatgt
    14461 ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata ctgaaagaat
    14521 cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat ccttattaac
    14581 acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata aggaggtaga
    14641 gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca agcgcagagc
    14701 tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg agggtaaaaa
    14761 tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt tccttatgaa
    14821 catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt acaaagcgcg
    14881 tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata tcttcatagc
    14941 ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat aatacaacca
    15001 gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg gtggcaaaaa
    15061 aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg atactaaagc
    15121 ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg gtcagcaggc
    15181 gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg aacaccctat
    15241 tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc ataacagttg
    15301 cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac gacaacgttg
    15361 tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc accaatggtc
    15421 acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat ggtgacgggt
    15481 ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt tgccgtgtag
    15541 atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca gacatcaagc
    15601 atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata gccatagtca
    15661 cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc agcgagaatt
    15721 tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat aagctgacga
    15781 gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc accagcaatc
    15841 atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg gcaacacagt
    15901 aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa gatcacatag
    15961 ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta tagctccgaa
    16021 agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg tcgtatagtt
    16081 tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc gccagttcat
    16141 tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca attgtctggc
    16201 aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac atacttctac
    16261 ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt cgcattgaca
    16321 aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg catattaata
    16381 ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg ctcaatgagc
    16441 ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat atgctcaatc
    16501 agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc atgattgctt
    16561 tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt aagctgtgcg
    16621 atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag cggttgaact
    16681 aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc tcgtttgtta
    16741 gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa attacaatta
    16801 ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag caggtaaaag
    16861 cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat tggtgacact
    16921 aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag aattaccgca
    16981 aataattaag aatagcctct aaatgattat ggattattaa atctattagc gctacttaat
    17041 ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat actggttttc
    17101 gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt atgcataaaa
    17161 aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg acagtatggc
    17221 tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca aagaataccg
    17281 gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca ccggtaccta
    17341 ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata acgccgagga
    17401 taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca tcaagcgtcg
    17461 cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg acgataagta
    17521 tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag taaacgtcat
    17581 tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc ggaacgacca
    17641 gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac aggaatacga
    17701 caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt tcctgatagg
    17761 acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt tgttccatca
    17821 gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt tccatggatg
    17881 gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa gaggagaacg
    17941 ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg atatcatccg
    18001 gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga ccgcggtcga
    18061 taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta atgtcgttga
    18121 tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac tgcggggtat
    18181 cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct gcttctgcaa
    18241 gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg tcaatcagct
    18301 caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta aaggcgaaaa
    18361 ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc gcttcaactt
    18421 tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca ataccggtat
    18481 tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca acgttgttct
    18541 tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta cgttcctgcg
    18601 ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca tctggcaaga
    18661 aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc agatacagca
    18721 ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag tggtgcgtgc
    18781 tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc ccgtgatcgc
    18841 ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc aacgctacca
    18901 gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag atagcaccag
    18961 tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc gcgataccga
    19021 ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc ggcaaacctt
    19081 cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc accaacaagc
    19141 cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca aaggcggcaa
    19201 ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc aacgtcgcgc
    19261 ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct tcgaccagcg
    19321 ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac gggtaaacaa
    19381 ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg attgccgcag
    19441 cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa gccggttggc
    19501 cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc gccacatcac
    19561 gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg aactcttcag
    19621 tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct ttcaccggcg
    19681 gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg gcggtaatga
    19741 catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag atacgcatcg
    19801 cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg atggcatctt
    19861 tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca tcggtgttga
    19921 taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc ccttgctgct
    19981 gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc tgaacctgcg
    20041 cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta cccgtggagt
    20101 cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt tcgataacct
    20161 gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg gagatcgtta
    20221 ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc gccagccccc
    20281 ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg ataaagaaat
    20341 taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg ctgagcaccg
    20401 cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg gacaccagga
    20461 cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc ttctgtcacc
    20521 agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac tttgtcatcc
    20581 gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc ctgttgcggg
    20641 actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa catacccggc
    20701 agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc agtggtctga
    20761 tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat gccgtcactg
    20821 gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt cgccagttcc
    20881 tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat cggatcaagt
    20941 tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc ttccgtcacg
    21001 ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc cagattgatc
    21061 cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg ttgcgcatca
    21121 gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag tttctgataa
    21181 cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag atcacctttc
    21241 gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga gacaccagct
    21301 tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac ttgaggacga
    21361 acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat ctgcagaggt
    21421 tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg ttgggcctgt
    21481 ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac cgccagaggc
    21541 gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa attggtcaat
    21601 ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat ggtacataca
    21661 ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca cgaaaaacca
    21721 aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt cttttctcac
    21781 agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc gttacgcgcg
    21841 gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc tgggaactgt
    21901 cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct ggcgatccac
    21961 tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca gaagaacgac
    22021 gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa atggctgttg
    22081 tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa caaacgttaa
    22141 aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg gcagcaatta
    22201 ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg caatcttttg
    22261 atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat ctcctgtgcc
    22321 ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag gatttttcct
    22381 ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc ggtttgactt
    22441 tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt ccagtattac
    22501 aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt cttgttatgc
    22561 cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa agcggacctg
    22621 caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga caaactggtg
    22681 cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat aaaagaagag
    22741 acagttcagc tacggcaaaa agtcgctgaa gcgccagaaa aaatgcgcca ggcgaccgcg
    22801 gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat tctgagtacg
    22861 ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt gcaaaacgca
    22921 caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca gcccgaacgc
    22981 gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag tcgtctggat
    23041 gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat gcaggctcag
    23101 caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg gaacaccgtc
    23161 ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc ccgtctggag
    23221 caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt aactgaaaaa
    23281 acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa tccgctggtg
    23341 aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc gactgaaaac
    23401 ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg ggcgctgcaa
    23461 tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct gttgtctcgt
    23521 atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa catgaccaac
    23581 cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga tgcactcttc
    23641 cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt caacagcgaa
    23701 gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga tcaactcaac
    23761 aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca gcagcagtta
    23821 atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg ggtgaacagt
    23881 aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa agatgaattt
    23941 aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt tatcgctttc
    24001 ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct gggctggctg
    24061 aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga tagccagctc
    24121 aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg cctgattatt
    24181 ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact gctatggtcg
    24241 ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa ggtactggag
    24301 aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag ccactggcgt
    24361 cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc tgtggtggca
    24421 gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat tttcttcaac
    24481 ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg tgataaagag
    24541 tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat tgcattgatg
    24601 gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg ttggattgaa
    24661 accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg cggcttaagc
    24721 gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa tctggtgaaa
    24781 gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga acaggttaac
    24841 cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt catgttctgg
    24901 gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac gctctggcat
    24961 tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg cagtctgttg
    25021 tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc tggtttactg
    25081 gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc cattactacc
    25141 atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc gctgggcgtc
    25201 tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg ttttggttta
    25261 caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg tccggtgcgt
    25321 attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat ccgtattcgt
    25381 gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa agcgtttgtt
    25441 accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt gatccgtctc
    25501 ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa ggcggcgact
    25561 gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc gtttggtgcc
    25621 agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg tagtcgtact
    25681 gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat caacattgcc
    25741 tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac ggaagtcaaa
    25801 cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa gggggcgaca
    25861 tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg cggcgtgacc
    25921 gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg tgtaggcctg
    25981 ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga catttagcgg
    26041 ccccatttat ttctcacttt tccgccttat catcgcgcgt taatttcttt tcataatcgc
    26101 gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt tcttccagca
    26161 gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt gacatactct
    26221 ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag cgcatggcgg
    26281 cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg ctgttccacg
    26341 agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg tagccgccgc
    26401 tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg tggcgcactg
    26461 tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag ttgtgccgcc
    26521 agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc atgacaaagc
    26581 gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc acgagtctga
    26641 aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg ggcacaacgc
    26701 tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt tttcacgata
    26761 agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag ctgacaacac
    26821 tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg ctgggtacgc
    26881 tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg gcctggtgct
    26941 ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg tttggcagct
    27001 atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg cgggcgattt
    27061 tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca tgggtgcgca
    27121 tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt ccggtgattg
    27181 atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt gcgtacagcc
    27241 agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac aactgttact
    27301 tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata cacttatgac
    27361 cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc aggactaccc
    27421 aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga aagcttacgc
    27481 tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca aagttgtcgg
    27541 caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg gcgttggctt
    27601 tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa cttacgacct
    27661 ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg gcgacaaagt
    27721 tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg ttaaactgat
    27781 ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt tcgatctcgg
    27841 cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc cgttcccggg
    27901 ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga tgtgcattca
    27961 gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca ccttccagcg
    28021 tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc acaaaccttt
    28081 gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt gtcgttaggg
    28141 cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac ctctatcgcc
    28201 cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc gtgcggcgtg
    28261 tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga aatcgacgcc
    28321 gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca gtacgctccg
    28381 gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc gcgccacagc
    28441 tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt tctgctggcg
    28501 acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca atttcatctc
    28561 aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa cgaagaacat
    28621 atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg cagcctgcga
    28681 gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt ttcaacccag
    28741 gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct ggttgaagcg
    28801 atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc tgcccgtggt
    28861 atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat tgcgatggta
    28921 caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg gatgcgtgaa
    28981 ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct gttgattggt
    29041 cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac gctgctgcgc
    29101 gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg ccagtccttt
    29161 gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca atcagcgccg
    29221 cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc ggcgcgccag
    29281 cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc agccgctacc
    29341 cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac cgatcgcgtt
    29401 caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga agcgtatcgc
    29461 tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc gaaggcgctg
    29521 aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc ggcagaagcc
    29581 attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa actggtcgaa
    29641 caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct gcatttgcgc
    29701 tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga agcgttgagc
    29761 atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc cgcggtgcgt
    29821 acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc gcgcgagtcc
    29881 attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga gctggatgaa
    29941 gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct taacgtgatt
    30001 gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc aagcccagca
    30061 gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag tcaccggcga
    30121 atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc gtcgcgtaga
    30181 gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg tggctgcagc
    30241 attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg cctctgtatc
    30301 ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac cagcccgctg
    30361 ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa gtcggcgcag
    30421 cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt ggcgcaggcg
    30481 ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt caccgaacag
    30541 gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat ctgtgtggtg
    30601 gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg tcgttatttt
    30661 gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat cgggcttgat
    30721 cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc caccaatccc
    30781 acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca atatgacgtg
    30841 gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat ggtcgatggc
    30901 accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc aaacgagagc
    30961 aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc ctcatcctgt
    31021 ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag aaactcgtgg
    31081 ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct attccaataa
    31141 agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca agctgcgttt
    31201 ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg ttcgtgtctc
    31261 tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga tgacccgcga
    31321 cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat tcctcgaatc
    31381 cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg ttggtttcta
    31441 ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag gcgaaaaacc
    31501 agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg ccgacatcac
    31561 caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag acgagttcct
    31621 cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg cgctgccggt
    31681 agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg agaaaatcaa
    31741 caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag agtacaaaga
    31801 gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc acaaccgcgt
    31861 tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc cgtgggatat
    31921 gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt tcatcatgga
    31981 cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga ttgactccag
    32041 cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa cgcgtaacct
    32101 gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga aagacgacgc
    32161 ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag gtccggcgga
    32221 agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta cccataccga
    32281 ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag aagggcagga
    32341 gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc cgcacctgga
    32401 actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg atgagtggat
    32461 gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta aagttgacga
    32521 gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg agaaagcact
    32581 gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag atgtccgtct
    32641 gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg aaatgagcac
    32701 tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga aatacatctt
    32761 cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag atgaagcgaa
    32821 gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac gcggcacgct
    32881 ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct gatgtcatgc
    32941 cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt ccgcaaatta
    33001 tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc tgttggtggt
    33061 atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg caatgcgtat
    33121 cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca tcatggagaa
    33181 atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca aatctggctc
    33241 cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca ccgacgaact
    33301 ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg gtttcctgtt
    33361 ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg gcatcaatgt
    33421 tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta tcgtaggccg
    33481 ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc cgaaagtaga
    33541 aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc aggaagaaac
    33601 cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg gctactactt
    33661 caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca agtcggttgc
    33721 tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca gatctgcttt
    33781 cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt tcttcttcct
    33841 cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat gcgtcagact
    33901 aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc tgaagcggta
    33961 aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc acggttgtta
    34021 tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt ggcgaagctg
    34081 tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg ccagcaacag
    34141 caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag ctacggctcg
    34201 ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca tattgtggtg
    34261 ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga tgaactggca
    34321 cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga ttacgccgat
    34381 aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc caaacatggc
    34441 gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc agatgaaggc
    34501 gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc actggggatg
    34561 gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc ctggctgatg
    34621 ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca tatacaggtg
    34681 atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc cgagcaaaac
    34741 cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc gcttaatgcc
    34801 acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta aagctgagcg
    34861 gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat ccgtgaataa
    34921 tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg ctgatgcgcc
    34981 gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc cgccccggca
    35041 ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta atacggtgac
    35101 tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc ctgttgcgtt
    35161 aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa tccgtaaagc
    35221 ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc acgcaaccac
    35281 aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc aatgcgggac
    35341 atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc cacaatttcc
    35401 tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc aatcaccgta
    35461 cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag attgccgaga
    35521 ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg cggacaaaag
    35581 agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt tgccatttct
    35641 ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg ctcagcaatc
    35701 gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac ggtcttcatt
    35761 tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca aaaactcctt
    35821 tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc tgctgatatt
    35881 gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc gctcagttaa
    35941 ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca ttacttccct
    36001 gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac cagcgccgcc
    36061 tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga tgatgaattc
    36121 attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga cgtagccgaa
    36181 gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc gggtggcacc
    36241 attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt gctgctgggc
    36301 gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg taacacttcc
    36361 agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg ttgctttacg
    36421 ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat gaaccagctg
    36481 cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt tctcacctct
    36541 tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa agccattgag
    36601 tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt tgtcattgcc
    36661 gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct tgcgatgaac
    36721 gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc tgacaaggcg
    36781 ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta tatggcgggc
    36841 tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg cgctatagcg
    36901 gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca gaatccgctg
    36961 cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat gaacactaat
    37021 ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa cagctaccat
    37081 cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta ttcatcgtta
    37141 gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca gcattcacct
    37201 cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta ctgggatcgt
    37261 taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg catttggctc
    37321 tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc atattttaca
    37381 tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt ccagcagttc
    37441 cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc cacgttcggt
    37501 gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat ccgggttttt
    37561 cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg tcaggatcag
    37621 ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg cattgcccaa
    37681 tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg tctcaatcac
    37741 caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta cacgaccgta
    37801 acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt cttcgattgc
    37861 ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct ccagtagtgc
    37921 gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca ggttttgtcc
    37981 ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg cgaactcacc
    38041 aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac caaacagacg
    38101 gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg tcgccagcac
    38161 tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg agacaaaaaa
    38221 cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt gactcagttc
    38281 agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga cgtcaaacaa
    38341 ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg taaatagctc
    38401 gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc gacctaccag
    38461 catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat cgactgcaag
    38521 agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca gcaacaccag
    38581 cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt gcccacgctg
    38641 actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac tggcggtgga
    38701 aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg cagagagcgc
    38761 catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg cgatggcctt
    38821 taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca acagaatgac
    38881 gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg gagtgaatgg
    38941 tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta gtttattggc
    39001 cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga taagcggggt
    39061 ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg ccgggaaaac
    39121 caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa taattattga
    39181 attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac agaaggcggt
    39241 ttaacagaga aaaggcttat ggcgactgta aaacgatgca gccaaagttt gcctttggct
    39301 gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca tccccaatag
    39361 tggcaggaaa gcacagattt tatagactaa ctcgatgctg g
//
LOCUS       DAJTYZ010000023        39165 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000023 DAJTYZ010000000
VERSION     DAJTYZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.023"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OO596_001164"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OO596_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6717071.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OO596_001165"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OO596_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6717072.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="OO596_001166"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="OO596_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6717073.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="OO596_001167"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="OO596_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6717074.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="OO596_001168"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="OO596_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6717075.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="OO596_001169"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="OO596_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6717076.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="OO596_001170"
     CDS             complement(6574..7338)
                     /locus_tag="OO596_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6717077.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OO596_001171"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OO596_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6717078.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="OO596_001172"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="OO596_001172"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6717079.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="OO596_001173"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="OO596_001173"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6717080.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="OO596_001174"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="OO596_001174"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6717081.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="OO596_001175"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="OO596_001175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6717082.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="OO596_001176"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="OO596_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6717083.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="OO596_001177"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="OO596_001177"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6717084.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OO596_001178"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OO596_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6717085.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="OO596_001179"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="OO596_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6717086.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="OO596_001180"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="OO596_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6717087.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="OO596_001181"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="OO596_001181"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6717088.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="OO596_001182"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="OO596_001182"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6717089.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="OO596_001183"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="OO596_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6717090.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="OO596_001184"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="OO596_001184"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6717091.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OO596_001185"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OO596_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6717092.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29322)
                     /gene="kefC"
                     /locus_tag="OO596_001186"
     CDS             complement(27550..29322)
                     /gene="kefC"
                     /locus_tag="OO596_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6717093.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVLVVLLGRYVTRPALRFVARSGLREVFSAVALFLVFGFGLLLEEVGLSMAMGAFLA
                     GVLLASSEYRHALESDIEPFKGLLLGLFFIGVGMSIDFGTLLENPLRIVILLLGFLII
                     KIAMLWLIARPLQVPNKQRRWFAVLLGQGSEFAFVVFGTAQMANVLEPEWAKSLTLAV
                     ALSMAATPILLVILNRLEQSSTEEAREADEIDEEQPRVIIAGFGRFGQITGRLLLSSG
                     VKMVVLDHDPDHIETLRKFGMKVFYGDATRMDLLESAGAAKAEVLINAIDDPQTNLQL
                     TEMVKEHFPHLQIIARARDVDHYIRLRQAGVEKPERETFEGALKTGRLALESLGLGPY
                     EARERADVFRRFNIQMVEEMAMVENDTKARAAVYKRTSAMLSEIITEDREHLSLIQRH
                     GWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29315..29845)
                     /gene="kefF"
                     /locus_tag="OO596_001187"
     CDS             complement(29315..29845)
                     /gene="kefF"
                     /locus_tag="OO596_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6717094.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(29953..31284)
                     /locus_tag="OO596_001188"
                     /pseudo
     CDS             complement(29953..31284)
                     /locus_tag="OO596_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31343..31630)
                     /gene="fixX"
                     /locus_tag="OO596_001189"
     CDS             complement(31343..31630)
                     /gene="fixX"
                     /locus_tag="OO596_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6717095.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31627..32913)
                     /locus_tag="OO596_001190"
     CDS             complement(31627..32913)
                     /locus_tag="OO596_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6717096.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(32964..33905)
                     /locus_tag="OO596_001191"
     CDS             complement(32964..33905)
                     /locus_tag="OO596_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6717097.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(33920..34690)
                     /locus_tag="OO596_001192"
     CDS             complement(33920..34690)
                     /locus_tag="OO596_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6717098.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35169..36683
                     /gene="caiT"
                     /locus_tag="OO596_001193"
     CDS             35169..36683
                     /gene="caiT"
                     /locus_tag="OO596_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6717099.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36714..37856
                     /gene="caiA"
                     /locus_tag="OO596_001194"
     CDS             36714..37856
                     /gene="caiA"
                     /locus_tag="OO596_001194"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6717100.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            37985..39165
                     /gene="caiB"
                     /locus_tag="OO596_001195"
                     /pseudo
     CDS             37985..39165
                     /gene="caiB"
                     /locus_tag="OO596_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9378 a        10759 c        10253 g         8775 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtaaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gggatcgccg cgatatcctg
    28861 gaacagcagc accgcaaagg cactgcgacc catttgcgtc accatcagat tgcgttcatt
    28921 catcgcctgc atggcaatcg ccgtagagga gagcgccagc gtcatgccga tcaattccgc
    28981 gacctgccag tgcagcccaa gtaacatgca gaacagcccc agcaggccgc cgcaaatcac
    29041 catctgcaat gcgccaccgc cgaacactgc cgcacgcagc ttccacagcc tttgtggatc
    29101 gagttcgagg ccgataataa acagcatcag caccacccca atctcggcaa agtgcagaat
    29161 agattcggca tcggtcacca gtcgcagccc ccacgggcca ataatgcagc cggcgatcag
    29221 gtagccaagt accgatccca gaccaagacg taccgcaatg ggtacaatca gcgctgccga
    29281 accgagataa atcagcgcct gaatcagcgt atggctatcc atgatgggcc tcctgccatt
    29341 ccagcagacg ttgcttatag tgacgcgcct gcccttcgag ggtttcgtcg tcacaaataa
    29401 aggtgcagtg catggcaaac ggtggcagcc agttcagccc gcagtagatt gccgtcgcct
    29461 gtagcggctg cgacagcaca tcaaagcccg gatgcgcacc aatttcaaaa tggctttccc
    29521 cgccgccggt cgtcaccgcc cacagcaaat atttgccatg cagcgccttg ccgccgtgac
    29581 cgtaagccca gccgtgcgac aaaactttat cgatccaaag tttaagaagc ggaggaatgc
    29641 tgtaccactg catcggatgc tgccagacga tcagatcggc gcgagacagc gcctcctgct
    29701 cggcggcaat atcgatattg aagtcaggat agagttgata aagagagcga atttcgacgc
    29761 cttccagcgt ccttgcctgt tcaagcatcc gtttattcgc atgggaatga tgcggatacg
    29821 gatgcgcata aattataaga atcataggta gcccgcgtga ttacgccttt gttttcccac
    29881 aaagtgtagt catatattca cgcggcttat agtcaataat attgattcag aaaagagaga
    29941 aatctgaaca atttacccca ttctctgccc gcggatcgtc atattgctgg tttgcgccag
    30001 tgacatccct cgagtctccg gggcaaacgc tacggaaatc aacaagccaa acagcgagat
    30061 acccgcctcc attagcatcg tgttactgat accgtaatta ttgataaaga tcggcagtgc
    30121 ccaggtcgaa acaatggtgc caatacggct taaggacata atcacgccca cggcagaggc
    30181 gcggatatct gtcgggaaga gttcattagg atagagccac tgcaaattac ccggcccgcc
    30241 agagaaaaag gcatacaccg caaaggccat cactaccagc cagatcccca tatccgggat
    30301 cagccccaaa accgccagcg ccagcgtcat catggcaaag ctgccaatca acaatggacg
    30361 ccgtccggca gtgtttaacc acagcatcgg cggaatacag ccgagcataa agaacaggct
    30421 aatcaccacg ttccccagcg ccgcgttttt gccaacaccc aatcccaaca aaccaacgat
    30481 ttgcgggcca aaggtgtaaa tggcgaacat tgggatcacc tggcaggtcc agatggcggc
    30541 aacaaacaga acaaaaggaa aatggcggcg attaaacaga tcgcgaaaac gggtttgcta
    30601 cggctgctct tcatcgaaag ccaccggttc gccaaacagt tttatcatca tctcttcgca
    30661 ctcttttact cgccctttgc gtaataacca gcggggagat tcaggcagtt cgaatcgacc
    30721 aatcaaaatc aacaaacagg ggatcgccgc gctacccagc atccagcgcc agccgccttc
    30781 cacatcataa agccagtagc cgaccagatc ggcgcaggtc gcgccgacat accacatggc
    30841 ggcgataaag ctgatggaaa atgcccgctg acgggtactg gagaactcgg tgatcattga
    30901 ggtggcgatg ggataatctg caccgatgac aatgccgata agtacccgca tcaccaacag
    30961 ttcgacgggg gatgaaacaa acatcgtcgc caccgatatc acgccgatgg cgatgatatc
    31021 aatgaggaac attttgcgcc gtcctacttt atcggaaata tagccaaaca gcgatgtgcc
    31081 aacgaacagc ccggcgagcg ttcccgcgcc caacaagcca atccagtcag cgtccagttt
    31141 tagcgccgga gtcagttgct ccagcgccac gccaatcatt accagtacat aaccatccag
    31201 aaacggacca ccgcttcccc acagcaaaat gcggcggtga attgaggaga atttgagatc
    31261 gtcaaagttt ctggacggtt gcatgggtta ttcctgtttt tttgcaatga gagtcagtac
    31321 ggcgttacgt atcaaaccaa catcagccgt aacggaactc cacaccaaag gtgccgcgcg
    31381 ggtattccca ctgttccagc gccgacccca gccccagaat gcgacaggtg ccgcactcca
    31441 gacatccggc gtaatcgaag cgcacactgc cgtcatcctg cttcttgtac agacctgcgg
    31501 ggcacgcttt caccagcagc tccagggcct gtttatcggc atcggccttt acaacaatgt
    31561 gcggatgctc ttcatcgaca ttgaatttat tgacgcccag tttgacgtcc acattgacgg
    31621 gagaagtcat aaaacggtca ctcctttcat gccatccttg atcagattga tgaagcccac
    31681 tttcttgccg tggcggagga ttttcttgcg catcagttcc ggcgcactgc cgtcaatggt
    31741 gaacaggtca cgcgccacgc ccaccgccag ttccgggtag ccgctaaaca tgcgtgggtt
    31801 atcaaggaag gccggtagtt tctggtacat acgcatatcg cgcagcgggc cactctcaag
    31861 atgctgacga tattccgcca gtttttgctt actgaaatcg tcgcttttca tcgctgaaag
    31921 cacggttttt gctgcggctt ccccggcggc aatcgccaga tccataccgc gaatggtaaa
    31981 accgaggttc atacacattc cggcggcatc accggcaatc aatacgccgt caccaaccaa
    32041 ctccggcagc atgttgatgc ctgcttccgg cactacgtga gcggaatatt ccaccagctt
    32101 gccgcccgcg atcagcggtg ccactgccgg atgctgtttg aaatcttcca gcatttgcgg
    32161 caccgatttt tttgcgtcat gcagatgatg cagaccacaa accagcccca gcgacagggt
    32221 attttcattg gtataaagga agccgccgcc catcaggcca tcggtgggtg atcccgcaaa
    32281 cagacaagcc gcgccctgat taccctgcaa ctgaaaacgg tcttcgatta ccgacttcgg
    32341 taactcgatc agttccttca cgccaaccgc cacatccgtc ggtttaacgc gttttgccat
    32401 ccccagcttt tcggcaagga tggagttcac tccatcagca aggatcaccg ttttcgcttc
    32461 aatcacatcg ccatcggctt ctacaccgac gactttgcca tcgcgctgta cgaggttatc
    32521 gacgcggatc ccggtaatta actgcgcgcc cgcttcttcg gcctgctcca tcagccaggc
    32581 atcaaattta ctgcgcaaaa cggagtaaga acgctgggat ggcgaggttt cgtcaccgtt
    32641 gcagtagtcc atagtcatcg ctgacttttc cgtcataaac gcgagttttt catgggtgat
    32701 caggcgttct accggggcgg attcggcgaa accaggaata atgtgttcca ggctgtgggc
    32761 atagagacgc ccgccggtga cgttcttggc acctgcggaa ttgccacgct cgataactaa
    32821 cacttgcgcc ccttcgcggg cgagcaccag tgcggcaacc gaaccggcaa gcccagcacc
    32881 gacgatgatg gcgtcaaaga tatcttcgga cataagagct ccctgtcagc ggcagggcca
    32941 aaatgcagcc ctgccagagt ggatcaacgc gctaaagctg cggtcagcgc cggaaggatc
    33001 ttcacggcgt caccaacaat gccgtaatcc gcgtactgga agatcggcgc atttttatct
    33061 ttattgatgg cgaaaatggt ttgcgatgca ttagcaccaa ccatgtgctg gatctgcccg
    33121 gagatcccca ccgccaggta cagttcaggt ttcagcatca ggttggagat accgacatag
    33181 cgttcgtgct ccatccattt ttcgttttcc gccaccggac gagaacaggc caactccgca
    33241 cctatcgcct tgcaaagctg ttctgccagc gcaatgttct ctttgctgcc aataccgcga
    33301 ccgacgctga ccaccagacg ggctttgtcg agatcgacgc tgttgctctg gcgcgcctgg
    33361 gttgccgtgc gggtaatcgc cacagccgga gcctgccact ccacggtgtg cgtttcgcca
    33421 gtgcgtgacg cgtctggctg agccgcatcg aacgtgccgc tgctgatggt cagtaccgca
    33481 tacggcgtgg caatgcgttc ttcgccaatc gccagaccac cgtaaaccat atgtttcact
    33541 gttgctttac cgtcctgtac gctgacggtg ctggcatcgt tagacaccgc cgctttaagg
    33601 cgataaccca gttttgccgc cagtaatttg ccgcgacggg tgtttggcag cagcaccagg
    33661 ccgtctgcgc cgtgctggcg aatagtgtca gccatgacac cggcgtaatc ttcgatcatc
    33721 cgatcgtccg gtttgccgtt taatttccag acatgattag cgccgagctg gattgcctgt
    33781 gcgccgtcgg catcattgag gacaaaggtg ttgatttgat tagctaaagc ctgcgcaccg
    33841 ttcatcagtt ccggcagacg agaaggggta tcgctgaata cccagacttg agaaaacgtg
    33901 ttcatagcat cccctgtaat taaatgactt tgcgaagatt ttcagcaaat gcggcgatct
    33961 gttcttcgcc gtcgccttca atcacgatgc gctgacgttc gcgctgtttc ggcgcggcaa
    34021 cctgttgttc tgaccaggct gcctctgcgt taaaaccaat atccgccgcc gaccatacct
    34081 ggacgggctt tttcgccgcg ccgagaatgg ctttcatcga aggaatttgt ggggaattga
    34141 tatcagtgga aacagcaaca accgcaggca gcggaatgct taaggtttcg gtttcatctt
    34201 ccagttcgcg ctcaacggtg agggtatctg ccgtcaggga gataattttg ctgacgccgt
    34261 taactgccgg aatattgagg atttcgccca ccagcagacc aacctgctgg gcataaaggt
    34321 cggaagaacc atcgccacag aggatcaaat caaagcctgc tttctgggcg gctgcagcca
    34381 gtgcgctcgc cgtttgttgc ggcagtgcct gctcgaactg gtcatcaatc accacaatca
    34441 gttcatccgg gccgcgcgat agtacatctt tacgcccttt ggcgttggtc agggctttac
    34501 cgcccacact taaggccgtc acctgcgcct ctgctgcctg ttgctttagc tggcaagccg
    34561 cttcaatagc gttcagatcg tattggctta ttttggcatc ggctttgctg aagtctaatg
    34621 aaccatcagc attattgacc gcaatatcct gttcatcagg cacgcactta tagcaagtaa
    34681 taatcttcat tgcatctcca gaaatcatga aggaaacatc gttcatatcg gtggtgttaa
    34741 ttacagatat cacgcccttt aatttggaaa taaaacttta atcaccaata ttgaaaatgt
    34801 cacacgcaga taaatccaac tttcaatatt gttaagttcc tcaccaatat tgaaaacatg
    34861 gcgtagaaaa aagaaatttt caatattgct ttatggatca ccaatattga aagtcagatg
    34921 ctattttttt actcacaaca caacataaca taacaaactg attaataatc aaaattaacg
    34981 agaaaagcgc cctctattca cccgaaaaaa aaatgtgata acaatcacag aatgcagctt
    35041 attgaatacc cattatgagt tagccattaa cgcgtccacg aggttaataa taattatatc
    35101 aaatgttaac aaaaataaaa caaacgggaa cgcaaaataa atattcgttt tcacagtgga
    35161 attaactcat gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc
    35221 cgttaataat cgtcggcata ctttgttggc ttacagtcag agatctggat gcagcgaatg
    35281 tcgttattaa tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt
    35341 atatggtggt gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc
    35401 gtttaggtaa cgaaccacct gaatttagta ccgccagttg gatctttatg atgttcgcct
    35461 cctgtacgtc tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca
    35521 ccccgccgtt tggcttagaa ccgaactcga caggggcgaa agagttgggg ctggcttaca
    35581 gcttgttcca ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg
    35641 cttacttctt ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc
    35701 tggtaggtga aaaacacgcc aaagggttgt tcggcactat cgtcgacaac ttctatctcg
    35761 tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt
    35821 gtatgcaatg gttgtttggc attccgcata ccctgcaact ggacgctatc atcattacct
    35881 gctggattat cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg
    35941 ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc attgtcagcg
    36001 gtgccagctt catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc
    36061 cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg
    36121 tgttctactg ggcatggtgg gtgatttatg ctatccagat gagtatcttc ctcgcccgca
    36181 tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat ggtgctgggg ctgacagcgt
    36241 caacctggat cctgtggact gtactcggta gtaacactct gctgttgata gataaaaaca
    36301 tcatcaacat tccaaatctg atcgaacagt acggtgtggc gcgcgccatc attgaaacct
    36361 gggccgctct gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg
    36421 ccaccgttac actggttaac gcctgctctt ataccctggc gatgtccact tgccgcgaag
    36481 tacgcgatgg tgaagaacca cctctgctgg tgcgtatcgg ttggtcaatt ctggttggca
    36541 ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg
    36601 ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg
    36661 cgaaacagaa ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt
    36721 ttaatttaaa tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg
    36781 aaaactggga ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg
    36841 tcaaagcact ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc
    36901 tggacgcggg gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac
    36961 caacctatgt gctgtaccag ttgccgggcg ggttcaacac cttcctgcgc gaaggcacac
    37021 aagagcagat cgacaaaatt atggctttcc gcggcaccgg taagcagatg tggaactcag
    37081 cgattaccga accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta
    37141 gaaatggtaa gatttatctt aatggtagta agtgttttat taccagcagc gcctacaccc
    37201 cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat
    37261 ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa acttgaaaag ctcggtctgc
    37321 gtatggatag ctgctgtgaa atcacctttg acgacgtgga actggacgag aaagacatgt
    37381 tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc
    37441 tggtagccct caccaactac ggtacggcga tgtgcgcttt tgaagatgcg gcgcgctacg
    37501 ccaatcagcg cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat
    37561 tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga
    37621 aagcagacaa cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca
    37681 atgcggcatt tgaagttgtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg
    37741 gcaaccaccg catcagccgc ttctggcgtg atctgcgtgt agaccgcgtc tccgggggat
    37801 ctgacgaaat gcagatcctg acgctgggtc gcgcggtgct gaagcaatac cgctaagtcc
    37861 gtgcgctgcc tgatgcgacg ctaatgcgtc ttatcaggcc tacgggagca cgaatgtagg
    37921 ccggataaag cgtttacgcc gcatccggca gtcatgcact acataaattt ttcaggagct
    37981 aataatggat catctaccca tgccgaaatt cgggccgttg gccggattgc gcgttgtctt
    38041 ctccggtatc gaaatcgccg ggccgttcgc cgggcaaatg ttcgcagaat ggggtgcgga
    38101 agttatctgg atcgagaacg tcgcctaggc cgacaccatt cgcgttcaac cgaactaccc
    38161 gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg
    38221 cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gcaaaggtcc
    38281 ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt
    38341 tatcgctcac ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc
    38401 ctataacacc atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca
    38461 gccaatgcct gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc
    38521 ggcgctggca gcactgcata aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc
    38581 catgtatgaa gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg
    38641 cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta
    38701 taaatgcgcc gacggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg
    38761 ctttaaagat attgacctcg cacatctact cggtacgccg gaaatcccgg aaggcactca
    38821 gcttatccac cgtatcgaat gcccttacgg cccactggta gaagaaaaac tcgatgcctg
    38881 gctggcggca cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg
    38941 tgccaaagta ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc
    39001 aatcactcag tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa
    39061 attcaaaaat aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc
    39121 tgccattttg aaaaatatcg gctacagcga aaacgacatt cagga
//
LOCUS       DAJTYZ010000024        39022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000024 DAJTYZ010000000
VERSION     DAJTYZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39022
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.024"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(159..371)
                     /gene="rpmE"
                     /locus_tag="OO596_001196"
     CDS             complement(159..371)
                     /gene="rpmE"
                     /locus_tag="OO596_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6717101.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            574..2772
                     /gene="priA"
                     /locus_tag="OO596_001197"
     CDS             574..2772
                     /gene="priA"
                     /locus_tag="OO596_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6717102.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2928..3953
                     /gene="cytR"
                     /locus_tag="OO596_001198"
     CDS             2928..3953
                     /gene="cytR"
                     /locus_tag="OO596_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6717103.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4045..5004
                     /gene="ftsN"
                     /locus_tag="OO596_001199"
     CDS             4045..5004
                     /gene="ftsN"
                     /locus_tag="OO596_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6717104.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5097..5627
                     /gene="hslV"
                     /locus_tag="OO596_001200"
     CDS             5097..5627
                     /gene="hslV"
                     /locus_tag="OO596_001200"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6717105.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5637..6968
                     /gene="hslU"
                     /locus_tag="OO596_001201"
     CDS             5637..6968
                     /gene="hslU"
                     /locus_tag="OO596_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6717106.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7035..7961
                     /gene="menA"
                     /locus_tag="OO596_001202"
     CDS             7035..7961
                     /gene="menA"
                     /locus_tag="OO596_001202"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6717107.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8054..8539
                     /gene="rraA"
                     /locus_tag="OO596_001203"
     CDS             8054..8539
                     /gene="rraA"
                     /locus_tag="OO596_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6717108.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8624..8869)
                     /gene="zapB"
                     /locus_tag="OO596_001204"
     CDS             complement(8624..8869)
                     /gene="zapB"
                     /locus_tag="OO596_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6717109.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9295..10140
                     /gene="glpF"
                     /locus_tag="OO596_001205"
     CDS             9295..10140
                     /gene="glpF"
                     /locus_tag="OO596_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR6717110.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10163..11674
                     /gene="glpK"
                     /locus_tag="OO596_001206"
     CDS             10163..11674
                     /gene="glpK"
                     /locus_tag="OO596_001206"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6717111.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11804..12814
                     /gene="glpX"
                     /locus_tag="OO596_001207"
     CDS             11804..12814
                     /gene="glpX"
                     /locus_tag="OO596_001207"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6717112.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12911..13657
                     /gene="fpr"
                     /locus_tag="OO596_001208"
     CDS             12911..13657
                     /gene="fpr"
                     /locus_tag="OO596_001208"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6717113.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="OO596_001209"
     CDS             complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="OO596_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6717114.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14117..14416)
                     /locus_tag="OO596_001210"
     CDS             complement(14117..14416)
                     /locus_tag="OO596_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6717115.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14628..15068)
                     /locus_tag="OO596_001211"
     CDS             complement(14628..15068)
                     /locus_tag="OO596_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6717116.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15169..15768
                     /locus_tag="OO596_001212"
     CDS             15169..15768
                     /locus_tag="OO596_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6717117.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            15876..16643
                     /gene="tpiA"
                     /locus_tag="OO596_001213"
     CDS             15876..16643
                     /gene="tpiA"
                     /locus_tag="OO596_001213"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6717118.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="OO596_001214"
     CDS             complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="OO596_001214"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6717119.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="OO596_001215"
     CDS             complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="OO596_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6717120.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="OO596_001216"
     CDS             complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="OO596_001216"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6717121.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="OO596_001217"
     CDS             complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="OO596_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6717122.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21062..21562)
                     /gene="cpxP"
                     /locus_tag="OO596_001218"
     CDS             complement(21062..21562)
                     /gene="cpxP"
                     /locus_tag="OO596_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6717123.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21712..22410
                     /gene="cpxR"
                     /locus_tag="OO596_001219"
     CDS             21712..22410
                     /gene="cpxR"
                     /locus_tag="OO596_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6717124.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22407..23780
                     /gene="cpxA"
                     /locus_tag="OO596_001220"
     CDS             22407..23780
                     /gene="cpxA"
                     /locus_tag="OO596_001220"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6717125.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23886..24560)
                     /gene="yiiM"
                     /locus_tag="OO596_001221"
     CDS             complement(23886..24560)
                     /gene="yiiM"
                     /locus_tag="OO596_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6717126.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24709..25692)
                     /gene="kdgT"
                     /locus_tag="OO596_001222"
     CDS             complement(24709..25692)
                     /gene="kdgT"
                     /locus_tag="OO596_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6717127.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25952..26572)
                     /gene="sodA"
                     /locus_tag="OO596_001223"
     CDS             complement(25952..26572)
                     /gene="sodA"
                     /locus_tag="OO596_001223"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6717128.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26857..27891
                     /gene="rhaT"
                     /locus_tag="OO596_001224"
     CDS             26857..27891
                     /gene="rhaT"
                     /locus_tag="OO596_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR6717129.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGAGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27888..28736)
                     /gene="rhaR"
                     /locus_tag="OO596_001225"
                     /pseudo
     CDS             complement(27888..28736)
                     /gene="rhaR"
                     /locus_tag="OO596_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(28810..29646)
                     /gene="rhaS"
                     /locus_tag="OO596_001226"
     CDS             complement(28810..29646)
                     /gene="rhaS"
                     /locus_tag="OO596_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6717130.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29934..31403
                     /gene="rhaB"
                     /locus_tag="OO596_001227"
     CDS             29934..31403
                     /gene="rhaB"
                     /locus_tag="OO596_001227"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6717131.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31400..32659
                     /gene="rhaA"
                     /locus_tag="OO596_001228"
     CDS             31400..32659
                     /gene="rhaA"
                     /locus_tag="OO596_001228"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6717132.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            32901..33725
                     /gene="rhaD"
                     /locus_tag="OO596_001229"
     CDS             32901..33725
                     /gene="rhaD"
                     /locus_tag="OO596_001229"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6717133.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33735..34049
                     /gene="rhaM"
                     /locus_tag="OO596_001230"
     CDS             33735..34049
                     /gene="rhaM"
                     /locus_tag="OO596_001230"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6717134.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34350..34796
                     /locus_tag="OO596_001231"
     CDS             34350..34796
                     /locus_tag="OO596_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6717135.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            34807..36258
                     /locus_tag="OO596_001232"
     CDS             34807..36258
                     /locus_tag="OO596_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6717136.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36248..37318
                     /locus_tag="OO596_001233"
     CDS             36248..37318
                     /locus_tag="OO596_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6717137.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37318..>39022
                     /locus_tag="OO596_001234"
     CDS             37318..>39022
                     /locus_tag="OO596_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCR6717138.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     LHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AG"
BASE COUNT         9495 a        10127 c        10142 g         9258 t
ORIGIN      
        1 gtaaaatcct tttgaaataa ataagaacca gaagcctagc gccagtcaac aacaggaaat
       61 gataatgagt taacaccccg gaatttttca gaatttataa aaggcaaatt acaggcacaa
      121 aaaaagcgcc gtgcggcgct tttttcggaa atccggtctt atttgctgcc cgggatgttg
      181 aaacgcttgt tgaagcggtc aacacggcca ccggtagcaa catcacgctg tttgccagtg
      241 aagaacgggt ggcacttgct gcacacgtcg aggttcaggt catgaccaac ggtggagcgg
      301 attttcatta cgttaccgca agagcagcta gcagtaattt cttcgtattt cgggtgaata
      361 tcttttttca tgggaaaacc tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag
      421 acaccacgcg atgttaaaag tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat
      481 catacagaaa ttaaccagcg tatgcaaact gatccgcact cttctacggc aatgtgtata
      541 ctaacccacc gaatttcaag tcaggatgat gctatgcccg ttgcccacgt tgccttgccc
      601 gttccgcttc ctcgtacctt tgactatctg ctgccagaag gcatggcggt taaagccggg
      661 tgtcgcgtgc gcgtgccgtt tggcaaacag caggagcgca tcggggttgt ggtatcagtt
      721 agcgatgtca gtgagctgcc gcttaacgag ctgaaagcgg tagttgaagt gctggacgtt
      781 gagccggtat ttactcactc cgtctggcga ttactgctct gggcggcaga ttactatcat
      841 catccgattg gcgatgtgct gtttcatgcc ttgccgattt tactgcgcca ggggcggcct
      901 gcggcgaacg cgccgatgtg gtactggttt gccactgaac aaggccaggc ggtggatctg
      961 aacagcctga aacgctcacc caagcaacaa caggcgctgg cggcgttacg gcaagggaaa
     1021 atctggcgcg accaggttgc cacgctcgaa tttaatgatg ccgcgttgca gacgctgcgc
     1081 aaaaaaggtc tgtgtgattt agcaagtgaa acaccagagt ttagcgactg gcgaacgaac
     1141 tatgccgttt ctggtgagcg gttgcgattg aataccgaac aggccaccgc cgttggcgca
     1201 attcatagcg cggcagatac tttttctgcc tggctgctgg cgggcgttac cggttccggt
     1261 aaaacggagg tttatctcag tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg
     1321 gtgatggtgc cggaaatcgg cctgacaccg caaactatcg cccgttttcg tgaacggttt
     1381 aatgcgcccg tggaagttct gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg
     1441 ctgaaagcga aaaatggcga ggcggcgatt gtgatcggca cccgctccgc gctgtttacg
     1501 ccgtttaaaa atctcggcgt gattgtcatt gacgaagagc acgacagttc ctacaagcag
     1561 caggaaggct ggcgctatca tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa
     1621 atcccgatta ttcttggctc cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag
     1681 aaaaaatacc gcctgctgcg cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa
     1741 cacgtgctgg atttaaaagg tcagaaggtg caggcaggtc tggctccggc gttaatcacc
     1801 cgtatgcgcc agcatttaca ggccaacaat caggttatcc tctttcttaa ccgccgtggc
     1861 tttgcgcctg cgctactgtg ccacgactgc ggctggattg ccgaatgccc acgttgcgat
     1921 cactactaca cgctgcatca ggcgcaacag catttgcgct gtcaccactg tgacagtcag
     1981 cgtccggtgc cgcgccagtg cccttcctgc ggttccacgc acctggtacc cgtggggctg
     2041 ggcaccgaac agcttgaaca gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt
     2101 atcgaccgcg ataccaccag ccgcaaaggg gcgctggaac agcaactggc ggaagtacat
     2161 cgcggcggtg cgaggatttt gatcggcaca caaatgctgg cgaaaggtca ccactttccg
     2221 gatgtgacgc tggtggcgtt actggatgtg gacggcgcgc tgttttctgc cgattttcgc
     2281 tcggcagagc gtttcgctca gctttacacc caggtcgccg gtcgtgccgg gcgtgcgggt
     2341 aaacagggcg aagtggtgct gcaaacgcac catccggaac atcctctgtt gcaaacgttg
     2401 ctctataaag gctacgacgc ctttgccgaa caggcgctgg ctgagcgacg catgatgcag
     2461 ctgccgccgt ggaccagcca tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg
     2521 ccgctgttcc tgcaacaact gcgtaatctg atcctctcca gcccgctggc agacgacaaa
     2581 ttgtgggttc tcggtccggt tccggcactg gcacctaaac gtggcggtcg ctggcgctgg
     2641 cagatattgt tgcagcaccc ttcccgcgtg cgcttgcaac acatcattaa cggtacgctg
     2701 gcactcatca atacaatacc ggattcccgt aaggtgaaat gggtgctgga tgttgatccg
     2761 attgagggtt aaaccgctca cgatgcgagg cggatcgaaa aattcaatat tcatcacact
     2821 tttcatgaaa attctgtaac cgttttcacg cgctatctgc taaaaatgtt gccgatgtga
     2881 agtaaacatg gatgtagtac gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga
     2941 agcaggaaac tgccgcgacc atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga
     3001 ctgtctcccg agcattaatg aatcccgata aagtctccca ggccacccgt aatcgggttg
     3061 aaaaagcggc ccgggaagtg ggttatttac cgcagcctat ggggcgcaac gtcaagcgta
     3121 atgaatcccg caccattctg gtgattgtcc cggatatctg cgatcccttc tttagcgaaa
     3181 ttattcgcgg tatcgaagtt acggcggcaa atcacggata tctggtgctg attggcgact
     3241 gtgcgcatca aaatcagcag gaaaaaacct ttatcgattt gatcatcacc aagcaaattg
     3301 atggcatgtt gctgctgggt tcaaggctgc cgtttgatgc cagcattgag gaacagcgta
     3361 atctgccgcc gatggtgatg gcgaacgaat tcgcaccgga actggagcta cctacagttc
     3421 atatcgacaa tctgaccgcc gcatttgatg cagtaaatta tctatatgag caagggcata
     3481 aacggattgg ctgtatagcc ggtcccgaag agatgccgct gtgtcactac cgcctgcaag
     3541 gttacgttca ggcgctgcgt cgctgcggca ttatggttga tccgcaatac atcgcccgtg
     3601 gcgacttcac cttcgaagcc ggaagcaaag cgatgcagca gctgcttgat cttccacaac
     3661 cgcctactgc tgtcttctgc catagcgatg tgatggcgct cggcgcactt tctcaggcaa
     3721 aacgccaggg gctgaaagtc ccggaagacc tttccataat cggttttgat aacatcgacc
     3781 tgacgcaatt ttgtgatccg ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc
     3841 gggaagctat gctgttattg cttgatcaaa tgcaggggca acacgttggc agtggctctc
     3901 gtttaatgga ctgcgaactt atcatccggg gatcaacacg cgcgttacct taaagtaaac
     3961 ctttaagact gccgtgactg gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga
     4021 acgaataaat acagcgaaac gatagtggca caacgagatt atgtacgccg cagccaaccg
     4081 gcaccttcgc ggcgaaaaaa gagcacctca cggaaaaagc aacgaaatct gcctgcggtt
     4141 tctcccgcta tggtcgctat tgctgccgcc gtgcttgtga cctttatcgg tggtctgtac
     4201 ttcattacgc atcacaagaa agaagagtcc gagacgctgc aaagccagaa agtgaccggt
     4261 aacggactac caccaaaacc agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc
     4321 cagccgggtg tgcgtgcgcc cacagaacct tctgccggtg gtgaagtgaa aacgccggag
     4381 caactgacac cagaacaacg tcagcttctt gaacaaatgc aggctgatat gcgccagcag
     4441 ccaacgcagc tggttgaagt tccgtggaat gaacagacgc cggaacagcg tcagcaaacg
     4501 ctacaacgcc agcgtcaggc gcagcaatta gcggagcagc aacgcctggc tcagcagtcg
     4561 cgtacgactg aacaaagctg gcagcagcag acgcgtacgt cgcaagccgc gccggtacag
     4621 gcgcaaccgc gccagtctaa accggcttct actcagcagc cgtaccagga tctgctgcaa
     4681 acccctgcgc acacgaccgc gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc
     4741 gctgacgcgc caaaaccgac ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc
     4801 ggttcgttca gaggcgcgga acaggcagag acggtacgtg ctcagctggc gttcgaagga
     4861 tttgactcga aaatcaccac caacaatggc tggaatcgtg tggtcattgg cccggtgaaa
     4921 ggcaaagaga acgcagacag caccctcaat cggttgaaga tggcgggtca tacaaactgc
     4981 attcggctcg ccgccggggg ttgaaaccct caaaatcccc cccatctata attgcattat
     5041 gccccgtact tttgtacggg gtttgtactc tgtattcgta accaaggggt cagctcgtga
     5101 caactatagt aagcgtacgc cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca
     5161 cgttgggcaa taccgtaatg aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca
     5221 aagtcatcgc gggctttgcg ggcggtactg cggatgcttt tacgctgttc gaactgtttg
     5281 aacgtaaact ggaaatgcat cagggccatc tggtcaaagc cgccgttgag ctggcaaaag
     5341 actggcgtac cgatcgcatg ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa
     5401 ctgcatcgct tatcatcacc ggtaacggtg acgtggtgca gccagaaaac gatcttattg
     5461 ctatcggctc cggcggccct tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg
     5521 aacttagcgc ccgtgaaatt gctgaaaagg cgttggatat tgcaggcgac atttgcatct
     5581 ataccaacca tttccacacc atcgaagaat taagctacaa agcgtaagga tctcccatgt
     5641 ctgaaatgac cccacgcgaa atcgtcagcg aactggataa gcacatcatc ggccaggaca
     5701 acgccaagcg ttctgtggcg attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg
     5761 aagagctgcg ccatgaagtg accccgaaaa atatcctgat gatcggcccg accggtgtcg
     5821 gtaaaactga aatcgcccgt cgtctggcta agctggcgaa tgcgccgttc atcaaagttg
     5881 aagcgaccaa attcaccgaa gtgggctatg tcggtaagga agtggattct attattcgcg
     5941 atctgaccga tgccgccgtg aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc
     6001 gcgctgaaga actggcagaa gaacgtattc tcgacgtgct gatcccacct gctaaaaaca
     6061 actggggaca gaccgaacag cagcaggaac cgtccgctgc tcgtcaggca ttccgcaaaa
     6121 aactgcgtga aggccagctt gatgacaaag aaatcgagat cgatcttgcc gcagcaccga
     6181 tgggcgttga aattatggct cctccaggca tggaagagat gaccagccag ctgcagtcca
     6241 tgttccagaa cctgggcggc cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca
     6301 tgaagctgct gattgaagaa gaagcggcga aactggtgaa cccggaagag ctgaagcaag
     6361 acgctatcga cgctgttgag cagcacggga tcgtgtttat cgacgaaatc gacaaaatct
     6421 gtaagcgcgg cgagtcttcc ggtccggatg tttctcgtga aggcgttcag cgtgacctgc
     6481 tgccgctggt agaaggttgc accgtttcca ccaaacacgg gatggtcaaa actgaccaca
     6541 ttctgtttat cgcttctggc gcgttccaga ttgcgaaacc gtctgacctg atcccggaac
     6601 tgcaaggtcg gctgccaatc cgcgttgaac tgcaggcact gaccaccagc gacttcgagc
     6661 gtattctgac cgagccgaat gcctctatca ccgtgcagta caaagcactg atggcgactg
     6721 aaggcgtaaa tatcgagttt accgactccg gtattaagcg catcgcggaa gcggcatggc
     6781 aggtgaacga atctaccgaa aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt
     6841 taatggaaga gatttcctac gacgccagcg atttaagcgg tcaaaatatc actattgacg
     6901 cagattatgt gagcaaacat ctggatgcgt tggtggcaga tgaagatctg agccgtttta
     6961 tcctataatc gcgttcaatc attttcatca ttgtttgatg gggctgaaag gccccatttt
     7021 tattggcgcg tattatgact gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt
     7081 tacgacctaa aaccctcccc ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat
     7141 ggtggcaagg tcacttcgat ccgctggtcg ccctgctggt actaattacc gccgggctat
     7201 tacagatcct ttctaacctc gccaatgatt acggcgatgc ggtaaaaggc agcgataaac
     7261 ctgaccgcat tgggccgcta cgcggcatgc aaaaaggggt cattacccag caagagatga
     7321 aacgggcgct cattattacc gtcgtgctca tctgtctctc cgggctggca ctggttgcag
     7381 tggcatgcca tacgctggcc gattttgtcg gtttcctgat tcttggcggg ttgtcgatca
     7441 ttgccgctat cacctacacc gtgggcaatc gtccttatgg ttatatcggt ctgggtgata
     7501 tttccgtact ggttttcttt ggctggttga gcgtcatggg gagctggtat ttacaggctc
     7561 atacattgat tccggcactg atccttccgg cgaccgcctg tggcctgctg gcaacggcag
     7621 tactgaacat taataacctg cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc
     7681 tggtggtgcg cttaggtgaa gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct
     7741 ctctggtatg tctggcgctg tttaatctct tttcgctgca tagcctgtgg ggctggctgt
     7801 tcctgctggc ggcaccatta ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc
     7861 cggtggcgat gcgaccaatg ctggaacgta ctgtcaaggg agcgttactg actaacctgc
     7921 tgtttgtttt agggatattc ctaagccagt gggcagcata actgacaaat atcaattaac
     7981 aattgatgat tttgccaaca gcctacatag cgcgatatac tgaaaattct cgcagcaact
     8041 gaatgttaag cctatgaaat acgatacttc cgagctttgt gacatctatc aagaagatgt
     8101 taacgtcgtg gaaccgctgt tctccaactt tggcggacgg gcgtcgtttg gcggacaaat
     8161 aatcacggta aaatgtttcg aggacaacgg gttgctgtac gatctgctcg aacagaatgg
     8221 ccgtggtcgt gttcttgtcg tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc
     8281 tgaactggcg cgtctggcag tacaaaatga atgggaaggt ctggttattt acggcgcggt
     8341 gcgtcaggta gatgacctag aagagttgga tatcggcatc caggcgatgg cggcaattcc
     8401 ggttggtgcc gctggcgaag gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt
     8461 aaccttcttc tccggcgacc atctttatgc cgacaatacc gggattattc tttcagaaga
     8521 tccgctggat attgaatgat aagaaaggca ccgcaaggtg ccttttttct gcgttacctg
     8581 ttggcctaca cagtaaagaa attacgcgaa agatgaagcg taatcagacc tcttccatgc
     8641 gacccagcag ggcctgcaga cgttcctgcc agccgttctg ctgttctttc agatggttgt
     8701 tctcacgctc cagctcttcg cgctgatgct gggcattttg aacttcctgc gacagtgagt
     8761 tgtttttttc tttcagctct tcgatttcca tctgcaacag agtgatggta tcaatcgcct
     8821 gctgtacttt tgcttccagt ttctcaaaca cttctaatga cattgtcata cctctcctga
     8881 attgcaaggc gttgatggat aaaaaatcct cgtcccgatt accggtgacg ccttaataaa
     8941 tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg tccagcggca
     9001 cgccttgcag attacggttt gccacacttt tcatccttct cctggtgaca taatccacat
     9061 caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc atcatgtaca
     9121 atcagatgga ataaattgcg cgataacgct cattttatga cgaagcacac acattttaag
     9181 ttcgatattt ctcgtttttg ctcgttaacg ataagtttac agcatgccta caagcatcgt
     9241 ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc gattatgagt
     9301 caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt
     9361 tttttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg
     9421 gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac cgcaggggtt
     9481 tccggcgcgc atcttaatcc cgctgttacc attgcattgt ggctgtttgc ctgtttcgac
     9541 aagcgcaaag ttattccttt tatcgtttca caagttgccg gcgctttctg tgctgcggct
     9601 ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca tcacattgtt
     9661 cgcggcagtg ttgaaagtgt tgatctggca ggcactttct ctacttaccc taatcctcat
     9721 atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct gatggggctg
     9781 atcctggcgt taacggacga tggcaacggt gtaccacgcg gccctttggc tcccttgctg
     9841 attggtctac tgattgcggt cattggcgca tctatgggcc cattgacggg ttttgccatg
     9901 aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc
     9961 gcctttaccg gcggcagaga cattccttac ttcctggtgc cgctttttgg ccctatcgtt
    10021 ggcgcgattg taggtgcatt tgcctaccgc aaactgattg gtcgccattt gccttgcgat
    10081 atctgtgttg tggaagaaaa ggagaccaca actccttcag aacaaaaagc ttcgctgtaa
    10141 tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc tcgaccaggg
    10201 caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca
    10261 gcgcgaattt gagcaaatct acccaaaacc aggctgggta gaacacgacc caatggaaat
    10321 ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata tcagttccga
    10381 tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct gggaaaaaga
    10441 aaccggcaag cctatctata acgccattgt ctggcagtgc cgtcgtaccg cagaaatctg
    10501 cgagcattta aaacgtgacg gtttagaaga ttatatccgc agcaataccg gtctggtgat
    10561 tgacccgtac ttttctggca ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg
    10621 cgagcgtgcg cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc ttatctggaa
    10681 aatgactcag ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta ccatgttgtt
    10741 caacatccat accctggact gggacgacaa aatgctggaa gtgctggata ttccgcgcga
    10801 gatgctgcca gaagtgcgtc gttcttccga agtgtacggt cagactaaca ttggcggcaa
    10861 aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggccg cgctgtttgg
    10921 tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat ggcactggct gctttatgct
    10981 gatgaacacc ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg
    11041 cggcccgact ggcgaagtga actatgcgct ggaaggtgcg gtgtttatgg caggcgcatc
    11101 cattcagtgg ctgcgcgatg agatgaagtt gattaacgac gcctacgatt ccgaatattt
    11161 cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggccttta ccgggctggg
    11221 tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc ggtctgactc gtggggtgaa
    11281 cgctaaccac attatacgcg cgacgctgga gtctattgct tatcagacgc gtgacgtgct
    11341 ggaagcgatg caggccgact ctggtatccg tctgcacgcc ctgcgcgtgg atggtggcgc
    11401 agtagcaaac aatttcctga tgcagttcca gtccgatatt ctcggcaccc gcgttgagcg
    11461 cccggaagtt cgcgaagtga ctgcattggg cgcggcctat cttgctggtc tggcggtcgg
    11521 tttctggcag aacctcgacg agctgcaaga gaaagcggtg attgagcgcg agttccgtcc
    11581 aggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag cggttaaacg
    11641 cgcgatggcg tgggaagaac acgacgtaat gtaaatgccg gatgaagcgt ttatgccgca
    11701 tccggtagtc ccgaaacgtg cgggggcaac cccgcacaca tcaataatcc ctcccttccc
    11761 ctgtgctaca cttcgcgcca ttccttactg cttagagttt gctatgagac gagaacttgc
    11821 catcgaattt tcccgcgtca ccgaatctgc ggcgctggct ggctacaaat ggttaggacg
    11881 cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca
    11941 ggtcaatatt gacggcacca tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct
    12001 ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg gtagatattg ctgttgatcc
    12061 gattgaaggc acgcgcatga cggcgatggg ccaggctaac gcgctggcgg tgctggcagt
    12121 aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat atggagaagc tgatcgtcgg
    12181 accgggagcc aaaggcacca ttgatttgaa cctgccgctg gcggataacc tgcgcaatgt
    12241 tgcggcggcg ctcggcaaac cgttgagcga actgacggta acaattctgg ctaaaccacg
    12301 ccacgatgcc gttatcgctg aaatgcaaca actcggcgta cgcgtatttg ctattccgga
    12361 tggcgacgtt gcggcctcaa ttctcacctg tatgccagac agcgaagttg acgtgctgta
    12421 cggtattggt ggcgcgccgg aaggcgtagt ttctgcggcg gtgatccgcg cattagatgg
    12481 cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg
    12541 tcgcattggc gagcaggagc tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt
    12601 attgcgcctg ggcgatatgg cgcgcagcga taacgtcatc ttctctgcca ccggtattac
    12661 caaaggcgat ctgctggaag gcattagccg caaaggcaat atcgcgacta ccgaaacgct
    12721 gctgatccgc ggcaagtcac gcaccattcg ccgcattcag tctatccact atctggatcg
    12781 caaggacccg gaaatgcagg tgcacatcct ctgattgatt tgatcgattg agccttccag
    12841 tccttcggga ctggaatttt tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca
    12901 ggagaaaaac atggctgatt gggtaacagg caaagtcact aaagtgcaga actggaccga
    12961 cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg tttaccgccg ggcaattcac
    13021 caagcttggc cttgaaatcg acggcgaacg cgtccagcgc gcctactcct atgttaactc
    13081 gcccgataat cccgatctgg agttttacct ggtcaccgtc cccgatggca aattaagccc
    13141 acgactggcg gcactgaaac caggcgatga agtgcaggtg gttagcgaag cagctggctt
    13201 ctttgttctg gaggaagtac cagattgcga aacgctatgg atgctggcaa ccggtacagc
    13261 gattggccct tatttatcga ttctgcaact aggcaaagat ttagatcgct tcaaaaatct
    13321 ggtcctggtt cacgccgcac gttatgccgc cgacttaagc tatttgccac tgatgcaaga
    13381 actggaaaaa cgctacgaag gcaaactgcg cattcagacg gtggtcagtc gggaaacggc
    13441 agcggggtcg ctcaccggac ggataccggc attaattgaa agtggggaac tggaaagcgc
    13501 gattggcctg ccgatgaata aagaaaccag ccatgtgatg ctgtgcggca atccacagat
    13561 ggtgcgcgat acacaacagt tgctgaaaga gacccggcag atgacgaaac atttacgtcg
    13621 ccgaccgggc catatgacag cggagcatta ctggtaagcg gttacttatc gataaacggc
    13681 acgatgagca aatccgcact catcttattg atcatcccgc gatatgccgg catcaaacgg
    13741 ttgataaatg agtggtgatg accacagaca aggaggtcgc actgctcttt ttgcataatt
    13801 tccagcagtg tttccggcat ttctccgcgt tcaatacgca gttttgtctt cggccattga
    13861 atatttttcg tcagtttata cagcttgtta tccgacttat tcttcaacaa ttgaagaata
    13921 tcttctgttg cagggaagta gatacccgga tacaactcgc ttaagccatc atcaatatga
    13981 attaacgtca ggtgagcgtc attatgtctg gcgagctcca gggctttatt caccagtaag
    14041 gcatcttctt cattcccgga aattgccacg ccaatgtgtt tataagccat gtttaactcc
    14101 ttctaaagca actccatcaa gctaacgaac gcagtgataa ctcaaaaata atcatctcgg
    14161 cctggcatga gaaagtgaag gccgcatcaa gctcaacttg cccctccaga tccttaatct
    14221 tataggccac gctggcccct tcatctgcag aggatgcagc ggcggcaagc tctttcagtt
    14281 tggtcataaa agactctgct tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac
    14341 agttgtcgtt atcaatcacc gtaccgatat caatcgcaca tcctttagta ctgcatttat
    14401 ctacgacatc tttcataggg cacctctgta tattcgcctc tctgtcgatt acgacttaag
    14461 aataatgcgc cgttaaggga agaacaaaca agtaatgatc ttatggatta ccattttttt
    14521 tgatattgct taaggtgaag agttcagaag gattttccgg gtattgtcat ttgcatcgtg
    14581 gaaataacaa tcccggaaaa aaatagcagt aagagatata ttaaaggtta attacttgat
    14641 ttattgtcgg ctttatactt cacatcctga gtatctttac catatttatt ttcgccttgt
    14701 gtgccaacaa acgcaccaag atcgataagc atcatcacca gaatcaacgt cgggacaaaa
    14761 cgccccaccg cccattgcca gacacccggt aaaatcgccc agttacccgc cagcagcatc
    14821 cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta
    14881 acagttactg ccgctgttgg ccaaagcaag cacacaaggc aaaacgccgc ggtctgaata
    14941 tcgagtaaat ttttacccgc cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct
    15001 atccaaatcc agaaatcacg gcgtccaata cgccctttaa atgagaataa ccattgctgt
    15061 atggtcatgt aagttccttg atggttgtct tttccaggat tctacccttt tgacaagggc
    15121 gacagatatc gttttaatcg gagccagtca taacaaaagg tactgtcaat gaagccaggg
    15181 tgtacgctgt tttttctctt atgttctgca ttaaccgtta caacaacggc gcatgcacaa
    15241 acaccagata cggcaacgac cgcgccttat ctgctggctg gagcccctac tttcgatctc
    15301 tccatcagcc agtttcgaga agactttaac agccagaatc ctagcctgcc actgaacgaa
    15361 tttcgtgcca tcgacagcag tcccgacaaa gccaatctca ctcgtgctgc aagtaaaatt
    15421 aacgagaact tgtatgcttc tacagcgctg gagcgcggta ccttaaaaat caaaagcatt
    15481 caaatgacct ggctacccat ccaggggcca gagcaaaaag cagcgaaagc gaaagctcag
    15541 gaatacatgg cagcagtgat ccgcacactc accccattaa tgaccaaaac acaaagccag
    15601 aaaaaactgc agtcgctact aacggcgggg aaaaacaaac gttattacac cgagacagaa
    15661 ggtgcactgc gttatgttgt cgcggacaac agcgaaaagg ggctgacctt cgctgttgaa
    15721 ccgattaagc tggcgctatc tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag
    15781 cctttgtgcc gatgaatctc tatactgttt cacagacctg ctgccctgcg gggcggccat
    15841 cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg acatccttta gtgatgggta
    15901 actggaaact gaacggcagc cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag
    15961 agctggcagg tgttgctggc tgtgcggttg caatcgcacc accggaaatg tacatcgata
    16021 tggcgaagcg cgaagctgaa ggcagccaca tcatgctggg tgcgcaaaac gtggacctga
    16081 acctgtccgg cgcattcacc ggtgaaactt ctgccgctat gctgaaagac atcggcgctc
    16141 agtacatcat catcggccac tctgaacgtc gtacttacca caaagagtct gacgaactga
    16201 tcgcgaaaaa attcgcggtg ctgaaagagc agggcctgac tccggttctg tgcatcggtg
    16261 aaaccgaagc tgaaaacgaa gcgggcaaaa ctgaagaagt ttgcgcacgt cagatcgacg
    16321 cggtactgaa aactcagggt gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg
    16381 tatgggcaat cggtactggc aaatctgcaa ctccggctca ggcacaggct gttcacaaat
    16441 tcatccgtga ccacatcgct aaagttgacg ctaacatcgc tgaacaagtg atcattcagt
    16501 acggcggctc tgtaaacgcg tctaacgctg cagaactgtt tgctcagcca gatatcgacg
    16561 gcgcgctggt tggcggtgct tctctgaaag ctgacgcttt cgcagtgatc gttaaagctg
    16621 cagaagcggc taaacaggct taagtctgac aggtgccgga tttcatatcc ggcacttact
    16681 ttccttaact cttcgcctta acgcaaaatc tcacactgat gatcctgaat ttcctcggct
    16741 gaagcacggt taagcgtcag tagatttcgt tgtgtcgcca gcaatacaaa tgagttatca
    16801 ctctgccgta ccatcgccag cccgtagctt cccatatgtt cccgcgcctc aggtacttct
    16861 tctgccagca tcataaatgg gctgcgttgt accagttcgc tttccgttac ccgacgcgcc
    16921 aggtattcat gcccacgcaa accacctggc agtggcaacc agcggctgct gatgttcgcc
    16981 agattgttat ccagttgttc gcgcacatca ggacgaatac aagagatatg aatatgaaaa
    17041 tggttttgcg tacgcccggt gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc
    17101 ggctggccgt attttttgct cataaaatca cgcgcctgcc aggccaacca aaagaagttc
    17161 ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt taatacgata cgttggcatc
    17221 aacagatatt gcagtggacc attaagatct tttaaaacca cgtatccggc attgggtttg
    17281 acttccgcac atggcgaagg attttgattt tgctgctgat tgggcaaaca ttcctcaagg
    17341 acaatcttac gtaatgtatc cgactcttca ccggttaatt tccagtaacc aataccggca
    17401 gccacaacgg cgataactat catcaccaaa aaaagaagac ccgctttttt catctttttt
    17461 tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt
    17521 cgcgagaaac caccgggttt gataattatc ctggggaaat cagcgtttgc tgatctgatc
    17581 gaacgtaccg ccgttagcaa aatgctcttt ttgcgctttc gtccagccgc cgaactcttc
    17641 atcaatggtg aataacttca gctttggaaa cgcattttcg tactttttcg ctacttcagc
    17701 gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga ccttctggcg agtagagata
    17761 tttcaggtag gcttccgcca cctctttggt gccttttttc tcgaccactt tatcgaccac
    17821 cgacacggtt ggctctgcga ggatagactc actcggcgtg acgatttcga atttatcttt
    17881 ccccagttca ttcgctgcca gcagagcttc gttttcccag gcgatcagta catcgccaat
    17941 cccgcgctcg acaaaggtgt tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt
    18001 tttatacagt gcccgaacaa aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag
    18061 cgcgtagccc caggcagcta ggtagttcca gcgtgcgcca ccagagcttt tcggattagg
    18121 cgtgatcacc gaaacacccg gtttaatcag atcgttccag tcatggatct gcttcggatt
    18181 gcccttacgc accaggaaaa caatggtgga agtgtacggt gcggagttat ccggcagacg
    18241 tttgatccac tctttatcaa tccgcccacg ttccgcaatc gcgtccacgt cataggccag
    18301 agccagcgtg acaacatcag cttcaatacc gttgattacc gacgtcgctt gtttacctga
    18361 accaccgtgt gactgacgaa tcaccacgtt atcaccagtt tgctgtttcc agtgggcgct
    18421 gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag
    18481 ctgaatatcc tttgccataa cgctggttgc cgccagcaaa aatgttaacc ctacgcccca
    18541 cttgttcatc gcccgactct cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt
    18601 aaccaaacat taaagaataa aaaaagattg gctataactt gcgggtatat gttgagggat
    18661 taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag tgcgctttgt ccatgccgga
    18721 tgcggcgcga acgccttatc cggcctacaa aagtttgcaa attcaataaa ttgcagaatt
    18781 catgtaggcc tgataagcga agcgcatcag gcatttttgc ttctgtcatc ggtttcaggc
    18841 taaaggaatc tgcctttttc cgaaatcatt aatacagttt tttcgcgcag tccagccagt
    18901 cacctttgaa cggacgcttc atgttttcga tagcgtcgat gatgtcgtgg tgaaccagct
    18961 gttcgttctg gatacctaca caacgaccgc cgtaacctgc cagcagcaga tcgatagcgt
    19021 aagcgcccat acgggaagcc agaatacggt cgtaaggcac cggagaacca ccgcgctgga
    19081 tgtggcccag cacagttgcg cgggtttcac gaccggtttc tttctcgatg aaatgcgcca
    19141 gttcgtcaac atcacacata tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg
    19201 cgatacccgc tttgatttcg tttaccaggt cttcacggct gaattcaact tccggaacca
    19261 caacgaattc acagccaccg gcaatggccg cagccaacgt cagatcgcca caataacggc
    19321 ccatcacttc caccacggaa atacgctggt gagaagaaga ggtgtcacgc agacggtcga
    19381 tcgcttctac aacggtgctc agcgcagtga agaaaccgat agtgtagtca gtgcctttga
    19441 tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa gcccatttcg gtcagacgca
    19501 ttgcacccat gtaggaaccg tcaccgccga taaccaccag cgcgtcgata ccacgttttt
    19561 tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg gaattccggg aagcgcgcag
    19621 aaccgaggaa cgtaccgcca cggttgatca tgtcagaaac gctgtaacgg tctagctgta
    19681 ccatacggtc ttcatacaga cccagatagc cgtcataaat gcccattact tccagacctt
    19741 ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc
    19801 cgcttgtcaa cacaccgatt ttcttaatca tgactacctc tgaactttgg aatgcaaaat
    19861 aaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt
    19921 ataacaatca cttcctgctg aattgattca ggtcaggcca aatggcggta ttttatacac
    19981 aaaatgcggg tctggctctc ttttatactg attatgaaag catagaccgt ttaccctccc
    20041 tgggtacgac ggaacagggg tcctgatgga taattacatc cgatcccgga aaacgccgta
    20101 aaatagcctg ctctacctga tccgccacca tatgtgcctg aaccaaaggc agagagtctt
    20161 ccatttccaa atgaatctga ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt
    20221 gagcgccgct aacacccggc caggaagtca cgatatcaat aatttcttgc cgttcctcat
    20281 caggcaatgc gcgatccagt aatgactgta ccgcctcata tcccatgcgt aacgcgctat
    20341 ataaaatata gatgccgatt cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc
    20401 aggacaaccc cagcgccagc agaattgcgc cgttcatcat aacatcagac tggtaatgta
    20461 gcatatcagc ccgcaccgcc tggctttgcg tccggcgcac cacccaccgc tgaaacgaga
    20521 caaggataat cgtacaaatt agcgccacaa ttgtcacgat aaccccgacg cctggatctg
    20581 tcatcggtgt tggagatacc agatgttgaa tacccgtcaa aaacaggaat agtgccgaac
    20641 cggagataaa catactttgc gccagcgccg cgagggactc agctttaccg tgaccaaacg
    20701 agtgattatc gtcggcaggt tgcagggaat atcgcaccac cagtaaattc gtcaacgacg
    20761 cgccgatatc caccagcgaa tccaccagcg cggcgagaat actcaccgac ccggtatacc
    20821 accatgcaaa aattttaatc agcaatagca gcgaagccat cgccgtcgca gcaatcgccg
    20881 cccgactgac cagccgtcca taagattgat tcataaatac tcccactatc aactgacgct
    20941 agtataacgg aagcaaatca tctgcaatgc attaagcagc aggcaaattg aggataaaaa
    21001 aaaccccaca tcatgtgggg gaagacaggg atggtgtcta tggcaaggaa aacagggttt
    21061 actactggga acgtgagttg ctactactca atagcttcaa cgatgaactt ttttgccatt
    21121 gcgtcacgtc acgcaactgc tccattcgtt gttgatgttt ctcgtttaaa accgcttgct
    21181 gctccggcgt taacaggcga tacatttggt tgcggacttt tgccatctca acctgacgag
    21241 caatttgctc attcgccatt ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt
    21301 ctgcggtgac aaggcgatgc attgtctcca gttcgctaac attaacagga ggctgttcgt
    21361 gccgggcctg ttgcataaga tctcgcatct gctgacgctg atgttcggtt aaacttatgc
    21421 cgtcgaacat atggctctgc gtactgcgct gcgtaagttc ttcacccgga tgccagttat
    21481 cgcctgaacc gacttcagca gcgtggctta atgaactgac tgccagcgtt gaggccatga
    21541 cggcagcggt aactatgcgc atcatttgct cccaaaatct ttctgtcgcg attcaacgat
    21601 agagagttta cgattcaggc tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca
    21661 tggattagcg acgtctgatg acgtaatttc tgcctcggag gtatttaaac aatgaataaa
    21721 atcctgttag ttgatgatga ccgagagctg acttccctat taaaggagct gctcgagatg
    21781 gaaggcttca acgtgattgt tgcccacgat ggggaacagg cgcttgatct tctggacgac
    21841 agcattgatt tacttttgct tgacgtaatg atgccgaaga aaaatggtat cgacacatta
    21901 aaagcacttc gccagacaca ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa
    21961 cttgatcgcg ttctcggcct tgagctgggc gcagatgact atctcccgaa accgtttaat
    22021 gatcgtgagc tggtggcacg tattcgcgcg atcctgcgcc gttcgcactg gagcgagcaa
    22081 cagcaaaaca acgacaacgg ttcaccgaca ctggaagttg atgccttagt gctgaatcca
    22141 ggccgtcagg aagccagctt cgacgggcaa acgctggagt taaccggtac tgagtttacc
    22201 ctgctctatt tgctggcaca gcatctgggt caggtggttt cccgtgaaca tttaagccag
    22261 gaagtgttgg gcaaacgcct gacgcctttc gaccgcgcta ttgatatgca catttccaac
    22321 ctgcgtcgta aactgccgga tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt
    22381 cgcggctatc tgatggtttc tgcttcatga taggcagctt aaccgcgcgc atcttcgcca
    22441 tcttctggct gacgctggcg ctggtgttga tgttggtttt gatgttaccc aagctcgatt
    22501 cacgccagat gaccgagctt ctggatagcg aacagcgtca ggggctgatg attgagcagc
    22561 atgtcgaagc ggagctggcg aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt
    22621 tccgggcgat tgataagtgg gcaccgccag gacagcgttt gttattggtg accaccgaag
    22681 gccgcgtgat cggcgctgaa cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg
    22741 ccgataacgc cgatcatccg cagaagaaaa agtatggccg cgtggaactg gtcggtccgt
    22801 tctccgtgcg tgatggcgaa gataattacc aactttatct gattcgtccg gccagcagtt
    22861 ctcaatccga tttcattaac ttactgtttg accgcccgct attactgctg attgtcacca
    22921 tgttggtcag tacgccgctg ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta
    22981 agctgaaaaa cgctgccgat gaagttgccc agggaaactt acgccagcac ccggaactgg
    23041 aagcggggcc acaggaattc cttgccgcag gtgccagttt taaccagatg gtcaccgcgc
    23101 tggagcgcat gatgacctct cagcagcgtc tgctttctga tatctctcac gagctgcgca
    23161 ccccgctgac gcgtctgcaa ctgggtacgg cgttactgcg ccgtcgtagc ggtgaaagca
    23221 aggaactcga gcgtattgaa accgaagcgc aacgtctgga cagcatgatc aacgatctgt
    23281 tggtgatgtc acgtaatcag caaaaaaacg cgctggttag cgaaaccatc aaagccaacc
    23341 agttgtggag tgaagtgctg gataacgcgg cgttcgaagc cgagcaaatg ggcaagtcgt
    23401 tgacagttaa cttcccgcct gggccgtggc cgctgtacgg caatccgaac gccctggaaa
    23461 gtgcgctgga aaacattgtt cgtaatgctc tgcgttattc ccatacgaag attgaagtgg
    23521 gctttgcggt agataaagac ggtatcacca ttacggtgga cgacgatggt cctggcgtta
    23581 gcccggaaga tcgcgaacag attttccgtc cgttctatcg gaccgatgaa gcacgcgatc
    23641 gtgaatctgg cggtacagga ttggggctgg cgattgttga aaccgccatt cagcagcatc
    23701 gtggctgggt gaaggcagaa gacagcccgc tgggcggttt acggctggtg atttggttgc
    23761 cgctgtataa gcggagttaa actccgcatt tgtaggcagg ataaggcgtt tacgccgcat
    23821 ccggcatttg agcaggatgc ctgatgcgac gctgatagcg tcttatcagg cctacactcc
    23881 gggggttatt ttccccacaa ccggcgggaa aaatcttcga tcttgccgct cagtcggcgc
    23941 ttttgcatcg tccgcgtcca gcttttcgat aacccggcgg cggagagtaa acggtgatac
    24001 tgatcgtcat caaacggcat atgccatgcg atggcggcag cctcctgcac ggtcacatcg
    24061 ctgacacggg aaaccaactc cagcggcgcg tccgcagaca ctttccctgg cgctatcacg
    24121 ctatacaacc accccacctt acctgtgttt tgcatcaact gcgctatatc gctgatatca
    24181 aaatggtaat tgagtttata gcaaggcgag cgcggctggc tgacctgaat taatgcctct
    24241 ccccagcgga aaatatcgcc catatgaaca ttactttccg tgagaccgtc ggttgagaga
    24301 ttttcgccaa acgcaggcgc aacaaacaac tccgcctgtt ccggaaattc ccgcacccag
    24361 tagagatagt gctcacgagg ataatgacac agcgctctgt ctggcccgcc gtgaaccttt
    24421 ttctccgcct gctcgtcacc ttccagcccc agctccgtca gcatcaactc accatcaacc
    24481 tggattttgg caattgcgct gggtttgcct tcgggataag cctgaatctt gcctgtgtat
    24541 acatcaaccg gatatcgcat ctgttccctc tccacgagaa atttccccat tagaccatat
    24601 ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc aggcaggaac tcaatagcaa
    24661 accggacgaa cgttagttca tccggctgtg gggattaagg aagcaggttt atttcaccgt
    24721 acctaaaatt tcgattttcg ctgctctggc tttgacttta cgtgaccaga tagaggtaag
    24781 aattggcacc agaatcgaag tcacaatgac cgccgtcgct accagcgaag ttgctgccgg
    24841 agccatcggt ttaaacgcag gcaccatttc tgcaatcagc acaggtgtcg ctaccgcggc
    24901 ccctgcggaa ctggaagcgg caattccggc agtgccatcg ccaccgccaa tcaatttatc
    24961 ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca ggatccccag
    25021 caaaccagtc tgggcaatca cagtcaaatc aatggtattg cccagcgcga aggcaaagaa
    25081 tggaatcagc gtttgcactg ctttgctgaa aaattctcgt aattcagggt caagattccc
    25141 cagggcaaag cccaccagga acggtaatac tgcgccgacg aaaacatgcg gttcaaacga
    25201 ggcaatcccg gcagtgccca gaataatcat cgtcatgagc ggcccggact ccaacgacat
    25261 caacacaaat gccccagctt cttctttcgt accgtactgc tgcatgatgg aagcgtaaag
    25321 tccgccgttg gtcatatcca tcgccgccac cagcgccagc gttgaaagtc cggcaaagaa
    25381 tccaacttca acaccatgtt ccggaataat gcgcgaggca attgccgcaa ccacccacgc
    25441 gacggcaatt ttagttacca ccagcgtacc ggatttacgc agtaccgttc ccgtcgcgct
    25501 taattttatt gacgccccca tgcaaaaaaa ccacaccgcc agaatgggca ccgtaccggt
    25561 aatcatcccg ttggtgaatg atccaaaata tttccccgcc cccggcgaga aggtgtggca
    25621 cagtgcgcca aggaatagcg ggacgagcat catccccccc gggattttct caatcgagcg
    25681 ttttatctgc atttccatca cctgtcggca tatttttaag gcctgatgcg taattcctca
    25741 atggagcatc agtgttgatg gaaacgaaga tagacattca tttaatcaat aaaagtgatc
    25801 aaaaccacat caattgaaac gctgttttat ttttatcgga tcattgtttt tattttgaaa
    25861 ttgatcacaa aaaaaccacc gccgttggcg atggttcatt ttttaagctg atatgcggcc
    25921 tatacgcctc attgcagcag gcggcaaatg attatttttt cgccgcaaaa cgtgccgctg
    25981 cttcgtccca gttcaccacg ttccagaact ctttaatgta gtccggacgg cggttctgga
    26041 atttcaggta gtaagcatgt tcccacacat ccaggcccag aatcgggaag ccggaagcgc
    26101 cagaaatagc ttcacccatc agcggagaat cctggttagc agtagaaacc accgccagtt
    26161 tatcgccttt cagcaccagc catgcccagc cggaaccaaa gcgggaagct gccgcttttt
    26221 caaattctgc tttgaagtta tcaacggagc cgaagtcacg ttcgatagcc gctttcaggt
    26281 caccctgcag ggtggtgcct tttttcagac ctttccagaa caggctgtgg ttagcgtgac
    26341 cgccagcgtt gttgcgcagt acggttttct tgtctgctgg cagctggtcc agtttggtga
    26401 tcagctcttc aaccggcagg ttggcaaatt ctggcaggct ttccagcgcc gcgttggcgt
    26461 tgtttacgta ggtctgatgg tgtttggtgt ggtggatttc catggtctgc ttatcgaagt
    26521 gcggttccag ggcatcgtaa gcatacggca gggatggcag ggtatagctc atattcatct
    26581 ccagtattgt cgggcggccg attgttaatg ccgcgtaagc agttggttca ttatagttaa
    26641 ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc gtaagggtat ggttttgcag
    26701 gaaaatgccc gagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc agtaaatcga
    26761 caacggcggc aacaggcgaa aggttaatcg acagcacgat ttttacactc atctcgtcgg
    26821 agatgtgaag cgacgaaaaa tgatgaggat aagaagatga gtaacgcgat tacgatgggg
    26881 atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgctcc gttcaaaaaa
    26941 gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc gtggattatt
    27001 ctgccgtgga ccatcagcgc cctgttacta ccggatttct gggcgtatta cagctcgttt
    27061 agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat cggtaatatc
    27121 aactatggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat cgccattggc
    27181 attacgttga ttgtcggtac gctgatgacg ccaattatca acggcaattt cgatgtgctg
    27241 attaacaccg aaggcggacg catgacgttg ctcggcgttc tggtggcgct gattggcgca
    27301 gggattgtaa ctcgagccgg gcagttgaaa gagcgcaaga tgggcattaa agccgaagag
    27361 ttcaatctga aaaaagggct ggtgctggcg gtgatgtgcg gcattttctc tgccgggatg
    27421 tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcact tggcgtcgat
    27481 ccactgtatg tcgctctgcc aagctatgtt gtcatcatgg gcggcggcgc gatcattaac
    27541 ctcggtttct gttttattcg tctggcaaaa gtgaaggatt tgtcgctaaa agccgacttc
    27601 tcgctggcaa aaccgctgat cattcacaat gtgttactct cggcactggg cgggttgatg
    27661 tggtatctgc aattcttttt ctatgcctgg ggccacgccc gcattccggc gcagtatgac
    27721 tacatcagtt ggatgctgca tatgagtttc tatgtattgt gcggcggtat cgtcgggctg
    27781 gtgctgaaag agtggaacaa tacaggacgc cgtccggtaa cggtgttgag cctcggttgt
    27841 gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta atctttctgc
    27901 gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa caccaccgaa
    27961 aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa caggcggcta
    28021 tgctggagaa gatattgcgc atgacacact ctgacctgtc gcagatattg attgatggtc
    28081 attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga tgcctcatca
    28141 caaaatttat ccagcgcaaa gggacttttc aggctagccg ccaaccgggt aatcagctta
    28201 tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg atggcgattc
    28261 agcaacatca ccaactgccc gaacagcaac tcagccattt cgttagcaaa cgacacatgc
    28321 tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat ccccacgcta
    28381 cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc tccctgtcag
    28441 tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac cagatcgtta
    28501 acggaagcgt aggagtgttt atcgtcagca tgaatataaa agagatcgcc acgggtaatg
    28561 cgataaggac gatcgttgag tacatgcaga ccattaccgc gccagacaat caccagctca
    28621 caaaaatcat gtgtatgttc ggcaaagaca tcttgcggat aacggtcagc cacagcgact
    28681 gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg cgccaccgtg
    28741 gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa tacgttgaga
    28801 agattcgcgt tattgcagaa agccatcccg tccctggcga atatcacgcg gtgaccagtt
    28861 aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccac agcgataggc
    28921 gatgtcagta acgctggcct cgctgtggcg tagcagatgt cgggctttca tcagtcgcag
    28981 gcggttcagg tatcgctgag gcgtcagtcc cgtttgctgc ttaagctgcc gatgtagcgt
    29041 acgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat cggcaaaatg
    29101 gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca ggttctcctg
    29161 caaactgctt ttacgcagca agagcagtaa ttgcataaac aagatctcgc gactggcggt
    29221 cgagggtaaa tcattttccc cttcctgctg ttccatctgt gcaaccagct gtcgcacctg
    29281 ctgcaatacg ctgtggttaa cgcgccagtg agacggatac tgcccatcca gctcttgtgg
    29341 cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat acagcacatt
    29401 ggtcagacac agattatcgg tatgttcata cagatgccga tcatgagcgc gtacgaaaca
    29461 gaccgtgcca ccggtgatgg tatagggctg cccattaaac acatgaatac ccgtgccatg
    29521 ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg cctgcgggag
    29581 ccggggttct atcgccacgg acgcgttacc agacggaaaa aaatccacac tatgtaatac
    29641 ggtcatactg gcctcctgat gtcgtcaaca cggcgaaata gtaatcacga ggtcaggttc
    29701 ttaccttaaa ttttcgacgg aaaaccacgt aaaaaacgtc gatttttcaa gatacagcgt
    29761 gaattttcag gaaatgcggt gagcatcaca tcaccacaat gaagctaatt gtgaacatca
    29821 tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac gagaaggtca
    29881 cgaattcagg cgctttttag actggtcgta atgaaattca gcaggatcac attatgacct
    29941 ttcgcaattg tgtcgccgtc gatctcggcg catccagtgg gcgcgtgatg ctggcgcgtt
    30001 acgagcgtga atgccgcagc ctgacgctgc gcgaaatcca tcgttttaac aatggcctgc
    30061 atagccagaa cggttatgtc acctggaatg tggatagcct ggaaagtgcc attcgccttg
    30121 gattaaacaa ggtgtgcgag gaagggattc gtatcgatag cattgggatt gatacctggg
    30181 gcgtggactt tgtgctgctc gaccaacagg gtcagcgtgt gggcctgccc gttgcttatc
    30241 gcgatagccg ctccaatggc ctaatggcgc aggcacagca acaactcggc aaacgcgata
    30301 tttatcaacg tagcggcatc cagtttctgc ccttcaatac gctttatcaa ctgcgtgcgc
    30361 tgacggagca acaacctgaa cttattccac acattgctca cgctctgctg atgccggatt
    30421 acttcagcta tcgcctgacc ggcaagatga actgggagta caccaacgcc acgaccacgc
    30481 aactggtcaa tatcaatagc gacgactggg acgagtcgct actggcgtgg agcggggcta
    30541 acaaagcctg gtttggtcgc ccgacgcatc cgggtaatgt cattgggcac tggatttgcc
    30601 cgcagggtaa tgagattccg gtggtcgccg ttgccagcca tgataccgcc agcgcggtta
    30661 tcgcctcgcc gttaaacggt tcacgcgctg cttatctctc ttctggcacc tggtcattga
    30721 tgggcttcga aagccagacg ccatttacca atgacacggc gctggcagcc aacataacca
    30781 atgaaggcgg ggcggaaggt cgctatcggg tgctgaaaaa tattatgggc ttatggctgc
    30841 ttcagcgagt gcttcaggag cggcaaatca acgatctccc ggcgcttatc gccgcgacac
    30901 aggcacttcc ggcctgccgc ttcatcatca atcccaatga cgatcgcttt attaatcctg
    30961 aggcgatgtg cagcgaaatt caggctgcgt gtcgggaaat ggcgcaaccg ctcccggaaa
    31021 gtgatgctga actggcgcgc tgcattttcg acagtctggc gctgctgtat gccgatgtgt
    31081 tgcatgagct ggcgcagcta cgcggtgaag atttctcgca actgcatatt gtcggcggcg
    31141 gctgccagaa cacgctgctc aaccagctat gtgccgatgc ctgcggtatt cgggtgatcg
    31201 ccgggcctgt tgaagcctcg acgctcggca atatcggcat ccagttaatg acgctggatg
    31261 aactcaacaa tgtggatgat ttccgtcagg tcgtcagcac caccgcgaat ctgaccacct
    31321 ttacccctaa tcctgacagt gaaattgccc actatgtggc gcagcttcac tcgacacgac
    31381 agacaaagga gctttgcgca tgaccactca actggaacag gcctgggagc tggcgaaaca
    31441 gcgtttcgcg gcggtgggga ttgatgtcga ggaggcgctg cgccaacttg atcgtttacc
    31501 cgtttcaatg cactgctggc agggcgatga tgtttccggt tttgaaaacc cggaaggttc
    31561 gctgaccggt gggattcagg ctattggtaa ttatccgggc aaagcgcgta atgccagtga
    31621 gctacgagcc gatctggaac aggctatgcg gctgattccg gggccgaaac ggcttaattt
    31681 acatgccatc tatctggaat cagacacgcc agtctcgcgc gaccagatca aacctgagca
    31741 cttcaaaaac tgggttgaat gggcgaaagc caatcagctc ggtctggatt ttaacccctc
    31801 ctgtttctcg catccgctaa gcgccgatgg ctttacgctc tcgcatcccg acgacagcat
    31861 tcgccagttc tggattgatc actgcaaagc cagccgtcgc gtttcggcct attttggcga
    31921 gcaactcggc acaccatcgg tgatgaacat ctggatcccg gatggtatga aagatatcac
    31981 cgttgaccgt ctcgccccgc gtcagcgtct gctggcagca ctggatgagg tgatcagcga
    32041 gaagctggat ccggcgcacc atatcgacgc cgttgagagc aaattgtttg gcattggcgc
    32101 agagagctac acggttggct ccaatgagtt ttacatgggg tatgccacca gccgccagac
    32161 tgcgctgtgc ctggacgccg ggcatttcca cccgactgaa gtgatttccg acaagatttc
    32221 cgccgccatg ctgtatgtac cgcagttgct gctgcacgtc agccgtccgg ttcgctggga
    32281 cagcgatcac gtggtgctgc tggatgatga aacccaggcg attgccagtg agattgttcg
    32341 tcacgatctg ttcgaccggg tgcatatcgg cctcgacttc tttgatgcct ctatcaaccg
    32401 cattgccgcg tgggtcattg gtacacgcaa tatgaaaaaa gccctgctgc gtgcgttgct
    32461 ggaacctacc gctgagctgc gcaagctgga agcggcgggc gattacactg cgcgtctggc
    32521 gctactggaa gaacagaaat cgttgccgtg gcaggcggtc tgggaaatgt attgccaacg
    32581 tcacgatacg ccagcaggta gcgaatggct ggagagcgtg cgggcttatg agaaagcgat
    32641 tttgagccag cgtgggtaaa cactgccgga tgcggcgcga gcgccttatc cggcctacag
    32701 gtcgaatgac catgcgtgct gccaccctca ccccggccct ctccctgaaa gggcgaggga
    32761 gaaaaacata gcctcgtgcg gcaacggagc tgaacaccgc tccggacagt cccctcgccc
    32821 ctacggggag agggttaggg tgaggggaac aagccgcacc atgtctaaac aaggcaacct
    32881 aatttacagg aacacagaac atgcaaaaca ttacccagtc ctggtttgtc cagggaatga
    32941 tcaaagccac caccgatgcc tggctgaaag gctgggacga gcgcaatggt ggtaacctga
    33001 cgctgcgcct ggatgacgcc gatatcgcgc cttatcacgg caactttcat gcacaaccgc
    33061 gctatattcc cctcagccaa cccatgcctt tactggcaaa tacgccgttt attgtcaccg
    33121 gctccggcaa attcttccgt aacgtccagc ttgatcctgc agctaactta ggcgtcgtaa
    33181 aagtcgacag cgacggcgcg ggctaccata ttctctgggg gttaaccaac gaagccgttc
    33241 caacttccga acttccggct cacttccttt cccactgcga gcgcattaaa gccaccaacg
    33301 gtaaagatcg ggtgatcatg cactgccacg ccaccaacct gatcgccctc acctatgtac
    33361 ttgaaaacga caccgcggtc tttactcgcc aactgtggga aggcagcacc gagtgtctgg
    33421 tggtattccc ggatggcgtt ggcattctgc cgtggatggt gcccggcacg gacgaaatcg
    33481 gccaggcgac cgcacaagag atgcaaaaac attcgctggt gttgtggccc ttccacggcg
    33541 tcttcggcag cgggtcgacg ttggatgaaa cattcggttt aatcgacacc gcagaaaaat
    33601 cagcacaagt attagtgaag gtttattcga tgggcggcat gaaacagacc atcagccgtg
    33661 aagagttgat agcgctcggc aagcgcttcg gcgttacacc actagctagt gcgctggcgc
    33721 tgtaaggagc aaacatgatc cgcaaagcct ttgtcatgca ggttaacccc gacgcccacg
    33781 aagagtatca gcgtcggcat aaccccatct ggccggaact ggaagcagtg ttgaaatctc
    33841 acggtgcgca taactacgcc atttacctcg acaaagcgcg taatctgctg tttgccacgg
    33901 tagagattga atctgaagaa cggtggaatg cggtagccag cactgatgtt tgccaacgtt
    33961 ggtggaaata tatgaccgat gttatgcccg ctaacccgga taacagcccg gtgagtagcg
    34021 agctgcaaga agtgttttac ctgccgtaat tgcccaatgt gccagagatg cctccggcac
    34081 aatattttaa atttcgattt gaaaaattaa acatattaca tctcccggta agttatattt
    34141 ccctgataca ttgtgagtaa atcacaaaaa taatgaataa cccattaatg attcatgtgg
    34201 tttatttaaa taaccattat gtgcattact ccgcaaatct gacctttcac tctgtctcaa
    34261 attgtcattg tttaccgaca tatcgcctct catcacttaa tcgctccacc attacaattg
    34321 attaaaatat attttatgga gtccgattaa tggcagctct tactgcaagc tgtattgacc
    34381 tgaatattca gggcaatggc gcttattccg ttctgaagca gttggcgaca atagcgttac
    34441 aaaacggttt tatcaccgac tcacaccagt tcctgcaaac cctgctgctg cgcgaaaaaa
    34501 tgcactctac tggatttggt tccggtgtcg ccgtgccgca cggtaaaagc gcctgcgtta
    34561 aacaaccgtt tgtattattc gcccgcaaag cgcaggctat tgactggaaa gccagcgatg
    34621 gcgaagacgt caattgctgg atctgcctcg gcgtgccgca aagcggcgaa gaggatcagg
    34681 tcaaaatcat cggcacactg tgtcgcaaaa ttatccacca ggattttatt catcaactgc
    34741 aacagggcga cgccgaccag gtgcttgcct tgttaaatca aaccctcagc tcataaggaa
    34801 gtggcgatgg agtcatcctt atgtattgtc gcgatcacca actgccccgc cgggatcgct
    34861 cacacctaca tggtggcgga agccctggaa cagaaagccc gttctctcgg ccataccata
    34921 aaagtggaaa ctcaagggtc cagtggcgtt gaaaaccgct tatccagcga agagattgcc
    34981 gctgccgatt acgtcattct cgctaccggg cgtggcctga gcggtgatga tcgcgcgcgg
    35041 tttgccggga agaaagttta tgagattgcc atctcccagg cgttgaaaaa tatcgaccag
    35101 attttcagcg aattaccgac aaactcgcag ctttttgccg cagatagcgg cgtgaagctt
    35161 ggcaaacagg aagtgcaaag cggcagcgtg atgagccacc tgatggcggg cgtctctgcg
    35221 gcgctgccgt ttgtcatcgg cggtggtatc ctggtggcgc ttgccaacat gctggtgcag
    35281 ttcggtttgc cgtataccga tatgtcgaaa ggcgcgccgt cgtttacctg ggtagttgaa
    35341 tccatcggtt accttggctt cacctttatg atccccatca tgggcgctta tattgcctcg
    35401 tcgattgccg ataaaccggc tttcgctccg gcgtttctgg tttgctatct ggcgaacgac
    35461 aaagcgctgc tcggcaccca gtcgggtgcg ggattcctcg gcgcggtggt gctggggctg
    35521 gcgattggct atttcgtctt ctggttccgc aaagtgcgcc ttggcaaagc attacaaccg
    35581 ctgctcggtt caatgctgat cccgttcgtt actctgctgg ttttcggcgt gctcacttac
    35641 tacgttatcg ggccggtgat gtccgacctc atgggcgggc tgctccactt cctgaatacc
    35701 attcctccat caatgaagtt tgcggcagcg tttctggtcg gtgcgatgct ggcgttcgat
    35761 atgggcggcc ccatcaataa aaccgcctgg ttcttctgct tctcactgct ggaaaaacac
    35821 atttatgact ggtacgccat cgtcggcgtt gtcgcgttga tgccgcccgt tgcggcgggt
    35881 cttgcgacgt tcattgcgcc caaactgttt acccgacagg aaaaagaggc tgccagtagt
    35941 gccattgtgg tcggtgccac agtcgcaacc gaaccggcta taccttacgc tctggcagct
    36001 ccgttgccga tgattaccgc caatacgctg gcgggtggca ttaccggcgt gctggtgatc
    36061 gctttcggaa tcaaacgtct ggcacctggt ttgggtatct ttgacccgct gatcggcctg
    36121 atgtcgccgg tgggttcgtt ttatctggtg ctagcgattg gcctcgcgct gaacatttca
    36181 ttcattatcg tcctgaaagg attgtggtta cgccgcaaag cgaaagccgc gcaacaggag
    36241 cttgtccatg aacattgagt tactgcaaca gttgtgcgaa gccagcgccg tcagcggcga
    36301 tgaacaggaa gttcgcgaca ttctgataaa cacgctggaa ccttgcgtta atgagatcac
    36361 ctttgatggt ctgggcagct ttgttgcccg taaggggaat aaaggtccaa aagttgccgt
    36421 tgtcaggcat atggatgaag tcggctttat ggtcacccac atcgacgaga gcggttttct
    36481 gcgctttacc accattggcg gctggtggaa tcagtcaatg ctcaaccacc gggtaaccat
    36541 acgcacacac aagggattta aaatccctgg tgtgattggt tccgtcgcac ctcatgcgtt
    36601 aacggaaaag caaaagcaac agccgctgtc atttgatgag atgttcattg atattggcgc
    36661 gaacagtcgc gatgaggtgg aaaaacgcgg tgtggcgatg ggcgatttta ttagcccgga
    36721 agccaatttt gcctgctggg gcgaagataa agtggtcggc aaggcgctgg ataaccgcat
    36781 cggctgcgca atgatggctg aactattgca gacggtgaat aatcccgaaa ttacgctgta
    36841 tggcgtcggc agcgtggaag aagaagttgg gctacgcggg gcacaaacct cggcggaaca
    36901 cattaaaccg gatgtggtga ttgtgctgga taccgctgtc gcaggtgatg ttccgggcat
    36961 tgataacatt aaatacccgc tgaaactggg ccaggggccg gggctgatgc tgtttgacaa
    37021 gcgctacttc cccaaccaga aactggtggc ggcgttaaaa agctgtgccg cacataacga
    37081 tttaccgctg caattttcca ccatgaaaac cggtgcgacg gatggcgggc gctacaacgt
    37141 gatgggcggc gggcgtccgg ttgttgcgct gtgcctgcca acccgctatc tgcacgctaa
    37201 cagcggcatg atttcaaaag ccgattacga tgctctgctc acgttgatac gggattttct
    37261 gaccacctta accgcggaga aagtcaacgc gtttagccag ttccgtcagg tggattaatg
    37321 ctaaacgaac gccagttaaa gattgtcgat ctgctggagc aacagccgcg cacgcctggt
    37381 gagctggcgc aacagactgg cgtttcaggc aggaccatcc tgcgtgatat tgactatctc
    37441 aactttaccc ttaacggcaa agcccgcatt ttcgccagtg gcagtgcggg ctatcagctg
    37501 gaaatcttcg agcgccgcag cttttttcag ttgctgcaaa agcacgataa cgacgatcgg
    37561 ctgctggcgc tgttattact gaataccttt actccccgtg cgcaactcgc ctcggcgctt
    37621 aatttgccag aaacctgggt agcagagcgt ctgccccggt taaaacagcg ttatgaacgc
    37681 acttgttgcc tggccagtcg ccccggtttg ggccatttca ttgatgagac agaagagaaa
    37741 cgcgttatct tgctggcgaa cttgctgcgc aaagatccgt ttttaattcc gctggcgggc
    37801 gtaacacgag acaattttca gcatttatcc acggcctgcg acaaccaaca ccgctggccg
    37861 ctcatgcagg gtgattatct ctccagcctg attctggcga tttacgccct gcgtaatcaa
    37921 ctgaccgatg agtggccgca atatcccggt aacgagataa aacaaatcgt tgaacagagc
    37981 ggcatgtttc ttggtgataa cgctgtaaga accctgacag gtttgataga gaaacagcat
    38041 cagcaagcgc aggtaatttc agccgatcat gtgctggggt tgctgcaaag ggtgccaggc
    38101 atcgcgtcat tgaatattat tgatacgcag ctggttgaga atattaccgg gcatttatta
    38161 cgttgccttg ccgcaccggt gtggattgcc gagcaccgcc aaagcagcat gaataacctg
    38221 aaagccgcct ggcctgcggc ctttgatatg agtctgcact ttattacgct actgcgtgaa
    38281 cagctcgata ttcccctttt cgatagcgat ctgatcggtt tgtattttgc ctgtgcgctg
    38341 gaacggcatc aaaacgaacg ccagccgatc attttgctct cggaccagaa cgcgattgcc
    38401 actattaatc agctcgccat tgagcgcgat gttttacatt gtcgggtgat tattgcccgt
    38461 agcttaagcg agcttattgc cattcgcgaa gagattgagc cgttattgat cattaacaac
    38521 agccattatt tactggatga cgcggtaaat aattacatca ccgtaaaaaa tatcattacg
    38581 gctgccgata tcgaacaaat aaaacacttt ctggcgacgg catttattcg ccaacagccg
    38641 gaacgctttt tctctgcccc cggaagtctt cattattcga atgtacgcgg tgaaagctgg
    38701 caacatatta cccggcaaat ttgtgcgcaa ttagtggcac aacaccatat taccgccgat
    38761 gaagcgcaac ggatcatcgc ccgcgaaggc gaaggtgaaa acctgattgt taatcgcctc
    38821 gcaatcccac attgctggag cgaacaggag cgacgttttc gtggattttt tattaccctc
    38881 gcccaaccag ttgaggtgaa taacgaagtc attaaccatg tcttgatcgc ctgcgccgcc
    38941 gccgatgcgc gtcatgagct aaaaatattt agctatctgg caagcgtatt gtgtcagcat
    39001 ccggcagaag ttattgccgg gt
//
LOCUS       DAJTYZ010000025        38549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000025 DAJTYZ010000000
VERSION     DAJTYZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.025"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO596_001235"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO596_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6717139.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OO596_001236"
     CDS             1946..2230
                     /locus_tag="OO596_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6717140.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="OO596_001237"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="OO596_001237"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6717141.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="OO596_001238"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="OO596_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6717142.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="OO596_001239"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="OO596_001239"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6717143.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7236..7799
                     /locus_tag="OO596_001240"
     CDS             7236..7799
                     /locus_tag="OO596_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6717144.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7881..10523
                     /gene="yfcU"
                     /locus_tag="OO596_001241"
     CDS             7881..10523
                     /gene="yfcU"
                     /locus_tag="OO596_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCR6717145.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10543..11294
                     /locus_tag="OO596_001242"
                     /pseudo
     CDS             10543..11294
                     /locus_tag="OO596_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11269..11817
                     /locus_tag="OO596_001243"
     CDS             11269..11817
                     /locus_tag="OO596_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6717146.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11814..12284
                     /locus_tag="OO596_001244"
     CDS             11814..12284
                     /locus_tag="OO596_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6717147.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12281..12805
                     /locus_tag="OO596_001245"
     CDS             12281..12805
                     /locus_tag="OO596_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6717148.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12792..13664
                     /locus_tag="OO596_001246"
     CDS             12792..13664
                     /locus_tag="OO596_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6717149.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="OO596_001247"
     CDS             complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="OO596_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6717150.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14503..15435
                     /gene="prmB"
                     /locus_tag="OO596_001248"
     CDS             14503..15435
                     /gene="prmB"
                     /locus_tag="OO596_001248"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6717151.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            15470..16555
                     /gene="aroC"
                     /locus_tag="OO596_001249"
     CDS             15470..16555
                     /gene="aroC"
                     /locus_tag="OO596_001249"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6717152.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16559..17383
                     /gene="mepA"
                     /locus_tag="OO596_001250"
     CDS             16559..17383
                     /gene="mepA"
                     /locus_tag="OO596_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6717153.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17383..18192
                     /locus_tag="OO596_001251"
     CDS             17383..18192
                     /locus_tag="OO596_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6717154.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18192..18740
                     /gene="epmC"
                     /locus_tag="OO596_001252"
     CDS             18192..18740
                     /gene="epmC"
                     /locus_tag="OO596_001252"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6717155.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISLWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18774..19052
                     /locus_tag="OO596_001253"
     CDS             18774..19052
                     /locus_tag="OO596_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6717156.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="OO596_001254"
     CDS             complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="OO596_001254"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6717157.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21338..22558
                     /gene="fabB"
                     /locus_tag="OO596_001255"
     CDS             21338..22558
                     /gene="fabB"
                     /locus_tag="OO596_001255"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6717158.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22833..24011
                     /locus_tag="OO596_001256"
     CDS             22833..24011
                     /locus_tag="OO596_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6717159.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(24008..25003)
                     /gene="flk"
                     /locus_tag="OO596_001257"
     CDS             complement(24008..25003)
                     /gene="flk"
                     /locus_tag="OO596_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6717160.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            25102..26238
                     /gene="pdxB"
                     /locus_tag="OO596_001258"
     CDS             25102..26238
                     /gene="pdxB"
                     /locus_tag="OO596_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6717161.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26304..27317
                     /locus_tag="OO596_001259"
     CDS             26304..27317
                     /locus_tag="OO596_001259"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6717162.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27317..28129
                     /gene="truA"
                     /locus_tag="OO596_001260"
     CDS             27317..28129
                     /gene="truA"
                     /locus_tag="OO596_001260"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6717163.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28212..28871
                     /locus_tag="OO596_001261"
     CDS             28212..28871
                     /locus_tag="OO596_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6717164.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            29027..29941
                     /gene="accD"
                     /locus_tag="OO596_001262"
     CDS             29027..29941
                     /gene="accD"
                     /locus_tag="OO596_001262"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6717165.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            30011..31279
                     /gene="folC"
                     /locus_tag="OO596_001263"
     CDS             30011..31279
                     /gene="folC"
                     /locus_tag="OO596_001263"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6717166.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31269..31931
                     /gene="dedD"
                     /locus_tag="OO596_001264"
     CDS             31269..31931
                     /gene="dedD"
                     /locus_tag="OO596_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6717167.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32190..32678
                     /gene="cvpA"
                     /locus_tag="OO596_001265"
     CDS             32190..32678
                     /gene="cvpA"
                     /locus_tag="OO596_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6717168.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32715..34232
                     /gene="purF"
                     /locus_tag="OO596_001266"
     CDS             32715..34232
                     /gene="purF"
                     /locus_tag="OO596_001266"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6717169.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34327..34896
                     /gene="ubiX"
                     /locus_tag="OO596_001267"
     CDS             34327..34896
                     /gene="ubiX"
                     /locus_tag="OO596_001267"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6717170.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35162..35944
                     /gene="argT"
                     /locus_tag="OO596_001268"
     CDS             35162..35944
                     /gene="argT"
                     /locus_tag="OO596_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6717171.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            36165..36947
                     /gene="hisJ"
                     /locus_tag="OO596_001269"
     CDS             36165..36947
                     /gene="hisJ"
                     /locus_tag="OO596_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6717172.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            37037..37723
                     /gene="hisQ"
                     /locus_tag="OO596_001270"
     CDS             37037..37723
                     /gene="hisQ"
                     /locus_tag="OO596_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6717173.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            37720..38436
                     /locus_tag="OO596_001271"
     CDS             37720..38436
                     /locus_tag="OO596_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6717174.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            38444..>38549
                     /locus_tag="OO596_001272"
     CDS             38444..>38549
                     /locus_tag="OO596_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine/lysine/arginine/ornithine ABC
                     transporter ATP-binding protein"
                     /protein_id="HCR6717175.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVIS"
BASE COUNT         9115 a         9455 c        10355 g         9624 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgtcgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgctgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatagttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgtgcgac ttaatgcccg taccgcctgc ggtagcagaa
     2941 tattctactg gcttgcggat gggcgacacc gccgaacaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgctc
     3361 ggtccagcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacgat gagcgaactg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgctcg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgcgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgatgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat tggcaactgc aaaacggcgc
     3961 aagaagcgga agcactggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg cggtgggctg gagctggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagtacagc attagagatg attctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagcc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggctatgac gctgaagctc gcgcctttgg cgaactggcg atgacgccgc
     4561 aatcgcaagc tttgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattaaaaca acagatggtg gcggaagtgg agcaaaattg cgccgctcat accatttttg
     4981 cttcgaacac gtcatcttta ccgattggtg atatcgccgc tcacgccgcg cgacctgagc
     5041 aagttattgg cctgcatttc ttcagtcctg tggaaaaaat gccgctggta gagattattc
     5101 ctcatgcggg tacatcggcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccgaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gcccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggcgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgaccttca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaacggacg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag cccttgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggcac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgagttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaatctca ctactatcga agactctccc catctataaa
     6661 taaaacactt tctactttca ttcggaaatt attcttctgc gcgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagaa aatagagttg aataatcagg caggatgtaa ttgaaacttg attaaattct
     6841 ggctatagtg tttatatatt atattgtcaa aagtggataa atgccaatcg cattatatgt
     6901 tcattgtttg ttttttaagc tgttgatatt cattattgat atactttcta ttttttctaa
     6961 atacttattc cgtatttttt aaggatgtcc ttaagataac ctttcttctg tcaatatatt
     7021 aattaaatct acatttactc tttagtaaaa ttaaattgca tcaggggtgt ggaaacatca
     7081 ctatgcggcc ccacaccaac gaatacaatt ttctacgacc tgttcttttt ggatcgcatg
     7141 ccgtattcaa tggctgggta taaacattgg ttattgactg aatcaatgta ttgcaatgtt
     7201 tatattttta attatttata gtttaaggac aaataatgag taagtttgtt aaaacagcta
     7261 ttgctgcggc aatggtgatg ggcgtgttca cctctacggc aacaatcgct gcgggtaaca
     7321 acggtactgc acgtttttac ggcaccattg aagactcagt gtgttctatc gttccggacg
     7381 atcacaaact ggaagttgat atgggtgata tcggtgccga aaaactgaaa aataacggca
     7441 ccaccacgcc gaaaagtttc cagattcgtc tgcaagattg cgtatttgat actcaggaaa
     7501 caatgaccac tacctttact ggtaccgttt cttctgcaaa tagcggcaat tattacacca
     7561 ttttcaatac cgatacctgt gcggcattta acaatgttag cctggcgatc ggtgactctc
     7621 tgggtacctc ttacaaaagc ggcatgggta ttgaccagaa aatagtgaaa gacacttcta
     7681 ccaataaagg caaagcgaag cagacactga actttaaagc ctggctggtc ggcgcagctg
     7741 atgcgccaga tctgggtaat ttcgaagcaa ataccacctt ccagattact tacctgtaag
     7801 gcataccccc atagcgacac tgctatgggg gatttaaatg gattttgacc gaacaagaat
     7861 aaaaacggat agaaacgtgt atgcctgacc attctctttt tcgattacgg attcttccgt
     7921 ggtgcattgc gctggcaatg tcagggagtt atagcagtgt ctgggctgaa gacgacattc
     7981 agtttgattc ccgttttctg gaattaaaag gcgacacaaa aattgatctg aagcgttttt
     8041 ccagccaggg atatgttgag cccggaaaat acaatttaca ggttcaacta aataaacagc
     8101 cattggcgga agagtacgat atttactggt atgctggtga agatgacgcg agcaaaagct
     8161 atgcttgtct gacaccggaa ctggtagcgc agtttggttt aaaagaagac gtggcgaaaa
     8221 atctgcaatg gagccacgat gctaaatgcc tgaaatccgg tcaactggaa ggcatggaaa
     8281 ttaaggctga tttaagccag tccgcattag tcatttcact gccacaggct tacctcgaat
     8341 atacttatcc cgactgggat ccgccttcac gttgggatga cggcatctcc gggatcgtcg
     8401 cggactacag catcaacgca caaacccggc acgaagaaaa tggcggtgat gatagtaacg
     8461 agatcagcgg caacgggacg gtcggggtta acctggggcc gtggcgtatg cgtgctgact
     8521 ggcagactaa ctatcaacat actcgcagta atgatgacga tgaattcagc ggcgatgaaa
     8581 ctcaaaaaaa atgggagtgg agtcgctact atgcctggcg ggcgttacca tcattaaaag
     8641 ccaaactggc gctgggcgag gattacctca gatccgatat ttttgatggt tttaactatg
     8701 ttggtggcag tgtcagtact gacgatcaaa tgttgcctcc caatctgcgc ggctacgcgc
     8761 cagacatttc cggcgtggca cacaccacag caaaagtgac cgtcagccag atggggcgtg
     8821 tgatttacga aacgcaggtt ccggctggac cgtttcgtat tcaggatctt ggtgattctg
     8881 tctccggtac gttgcatatt cgcattgaag aacagaacgg ccaggtgcag gaatatgaca
     8941 tcagcaccgc ctcgatgcca tacctcactc gtccaggtca ggttcgctat aagatcatga
     9001 tgggccgtcc gcaagagtgg ggacaccatg tcgagggtga atttttttct ggtgctgaag
     9061 cttcctgggg gatcgctaac ggctggtcgt tatatggcgg cgcactggga gatgaaaact
     9121 atcagtctgc ggcgcttggc gtcggtcgcg atttgtctac attcggcgcg gtcgcgtttg
     9181 atgttactca ctcgcacacc aaactggata aagacaccgc ttatggcaaa ggttcgctgg
     9241 acggtaactc cttccgtgtg agttattcca aagactttga ccagctcaac agccgcgtta
     9301 cttttgctgg atatcgcttc tcggaagaga actttatgac catgagcgag tatctggatg
     9361 ccagtgacag cggaatggta cgcacgggca acgacaaaga gatgtacact gccacttata
     9421 accagaactt ccgcgatgcg ggtgtttcgg tttatctcaa ctatacccgc catacctact
     9481 gggatcgcga ggagcagaca aactacaaca tcatgctctc gcactatttc aatataggca
     9541 gtattcgtaa tgtcagcatc tcgatgactg gctaccgtta cgagtatgac aaccaggccg
     9601 acaaaggcat gtacatttcg ctcagtatgc cgtggggtga caacagtacc gttagctata
     9661 acggtaacta tggcagtggg acggacagca gtcaggtcgg ttatttcagc cgtgtcgatg
     9721 acgcgactca ctatcagttg aacgtcggca ccagtgacaa acacaccagc gttgacggct
     9781 attacagcca tgatggttcg ctggcgcagg ttgacctcag tgcgaactac catgaagggc
     9841 aatacacctc tgcgggcttg tcgttacagg gcggcgcaac gcttactgcc cacggtggcg
     9901 cacttcaccg tacccagaat atgggcggga cacgcttgtt gattgatgcc gatggcgttg
     9961 ccgatgttcc ggtagaaggt aacggtgctg ctgtttatac caatatgttt ggtaaagccg
    10021 tcgtttctga cgtcaataac tattaccgca atcaggcgta tatcgacctc aacaaactgc
    10081 cggaaaacgc cgaagcaacc cagtcggtgg tacaagccac gctaactgaa ggggcgattg
    10141 gctaccgcaa atttgccgtc atcagtggtc aaaaagcgat ggcggtgctg cgtttacaag
    10201 acggcagcca tcctccgttt ggcgcagaag taaaaaatga taacgagcag acagtgggcc
    10261 ttgtcgatga tgacggcaat gtttatctgg ctggggtgaa acctggcgaa cacatgagtg
    10321 tgttctggag tggtgttgcg cattgcgata tcaacctgcc ggacccgctg cctgccgatc
    10381 tgtttaacgg cttgttactg ccatgccagc ataaaggcaa cgtcgcgccg gttgttcctg
    10441 atgacataaa gcctgtcatt caggagcaga cgcaacaggt gacacccacc aatccccctg
    10501 tttctgtttc agctaaccaa taacgtgatt aaggaatgat ccatgtctga tttactttgc
    10561 tctgccaaat tgggtgctac gacgctagcg ttactgctta gcgctgccag tttgtcgccc
    10621 aggcttctgt aacgccagat cgtacccgtt tgatatttaa tgaaagcgac aaatcaatca
    10681 gcgtgacgtt gcgcaataac gatccgaaac tgccttatct ggcccaaagc tggatcgaag
    10741 atgaaaaggg taacaagatc agttcaccgt taactgttct gccaccggtg caacgcatcg
    10801 attcaatgat gaacgggcag gtaaaagtgc agggaatgcc ggatatcaac aaattgcctg
    10861 ccgatcgtga aagcctgttc tacttcaacg tgcgtgagat cccgccaaaa tcaaacaaag
    10921 ccaacacgct gcaaatagca ctacaaacgc gtattaagtt gttctggcga ccgaaggcgc
    10981 tggagaacgt cagcatgaaa aacccctggc aatataaggt tactttaacc cgcaacggtc
    11041 aggaatttac cgtcaataac ccgacgccgt attacgtcat tttcagtaat gccagcacac
    11101 agaaaaatgg caatcctgca gccggtttct cgccaatagt gatgtcgccg aaaacctctg
    11161 caccgctgaa tgtgaagatg ggtagcgtgc cggtactgac ctatgtcaac gactatggcg
    11221 ctcggatgcc gctctttttc acttgcaacg gtaacagttg ccaggtcgat gaagagcaga
    11281 gccgcaaagg ctgaggccgg gagagccagt atgaaaggat cttgttctgt ctgggcggta
    11341 actgggttac tgatatgttg tgttggtagc ggctttgcag ttgcagcgag taagtcagta
    11401 agtatgaatt tacgggtgct tgtcgatgcc cctcctcctt gtacggtaaa tggtgcggca
    11461 gttgagtttg gtaatgtatt cattaataag atcaatgggg ttgattataa acgtcccatt
    11521 gactactcgc ttgtttgtaa caatctggca atggatgacc tgcgtctaca gatgcaggcg
    11581 acaacggtcg tgattaacgg tgaaacagtt atcagcacgg gtattcctgg gtttggtatt
    11641 cgggttcaga aaagtagtga tcacacaata ctcgacttaa cctctggctc atggttgcca
    11701 tttaattttt cctcgggagt tcccgttctg gaagctgtgc cggtgaaaca gagtggtaca
    11761 acgttagcgg cagctgaatt taacgccagt gccacaattg tggtggatta ccaatgaaac
    11821 ggatctcgct tattttgctg tggggctttt gctcgatggc actctcaaac gtctcttttc
    11881 acggttattt agtgcagccg cctaactgca cgattagcaa cgctcaaacc attgaaataa
    11941 cttttcagga tgtgcttatt gatgacatta atggcagcaa ttatgagcaa accgtgccgt
    12001 attccattac ctgcgatacg gcggttcgcg acccattaat ggaaatgacc ttgagctgga
    12061 gtggcacact aagcgatttt gataatgctg cagtaagcag caatatcact ggtttaggca
    12121 ttcagctcaa acaggcaggg cagagtttta cgatcaatac gccacttatg gtgaatgaaa
    12181 ccgatttacc ggtgttaacg gctgtgccag taaaaaagag tggtgtcgtt ctgccagagg
    12241 cggactttga ggcctgggcc acgttacagg tagattacca atgaagaaaa agcgtacgtt
    12301 gttttttatt agtagcctga tgttgctggg cagtggaacc acaatagcag gagataattt
    12361 gcactttacc ggcaatttaa tcagtaaatc gtgtacgcca gtgattaatg gtagccagtt
    12421 ggcagaagta cattttcctg ccattgccgc cagcgattta atgaaccttg ggcagtcaga
    12481 gcgagtgcca ttggtgtttc aattgaaaga ttgtcatagt tctacattgt ttaatgtcaa
    12541 agtaacgttg acaggaacag aagatagtgc tctacccggc tttctggcct ttgactcctc
    12601 gtcatcagca tccggcgctg ggattggtat tgaaactgcg gcaggcacat cggtgccaat
    12661 caacaataca acaggcgtca cgctcccgct gaatcaggga aataacagcc taaattttaa
    12721 tacctggtta caggcaaaga gcgggcgaga tgtaacgtcc ggagacttta gtgccacagt
    12781 gacggcaacc tttgagtatt tctgaaatga aaaaatggac cataattttc gcgagcctaa
    12841 tgctgctcgt tctgagtgtt gtcggcgcat ctaagagtta tgctggagat aaacttatga
    12901 gtgcgagttt tgacagtgtg agagtttatt atgccatgga caaggtaacc ggtgctgtag
    12961 cgagcagtgt tttcaatgtt acggtgataa cgccaaagga ggttacatat gggaaatatg
    13021 attcttttgc gtataaaggc gatacgttgc gggttatttc ctggtctggg tcgggttcag
    13081 ctccaacttt ggtactgact gactttgata caattaataa ctcaaattgc cctggcatag
    13141 atacgaaaat ttttcgttgt gcatatatga cgtttaaaat taccgttgca tctgatgatt
    13201 atggttgccc ttggatcgca tccttttatt cctatacaga tttacccgga tttggaagct
    13261 atacggcccc tacagtgcat aacacaatat gcccaacgat accggtcact tcatacgata
    13321 tctcctggag tgaaaattat gtcagccata ataaggcgtt acgaattcag tccacggggt
    13381 caacagtgac caccacgctt tcgacctacc tgatggaagg cggcagactc tgtgatggca
    13441 gtaatttttc tgataatgat ggcagaggag catactgccg cgcggtatca gaattgttaa
    13501 cctttacctc ttacggttgt gataaatcaa cggtaacggt tacgccaact cgtcatccgg
    13561 taacggataa agtactacac gatattgtgg tgaatgtgaa taccagtagt gggcaaccga
    13621 ttgattcgac gtgtcgtttt cagtatgtat tgaatgaact gtagttcgta gtattgctct
    13681 gatgcgtctt atcaggccta cgaagtgaat tgcaatttat tgaattatca catatttgta
    13741 ggccggataa ggcgtttacg ctgctccggc atttgctgcc agcattcagg gcaactccgg
    13801 cggcaaccac tcttccactt caatcaacac caacaaagcc gcatcaccgc cgtactcttt
    13861 cggtgcctgg tgaaatgcca tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg
    13921 cttcaaaata tgcttcccgt gcccatgcat cacgcaggca caaaacacat gttcgcggcg
    13981 acaggcggca atcaacgcgc ccagttcctg cttggcctgc agctgcgtca gaccgtgtaa
    14041 atccaaaaac aattccggtg aataatcgcc gcggcgcagt ttcttcgcct caaaatggct
    14101 gacatccggg cgaacatatt tcaccggacc ttcggtattt aataacggct gaaactcatc
    14161 ggagaaataa tggctggcat cagcctgctc cgggatcaag cgtttcaccg gcacttcgct
    14221 gattttttta cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc
    14281 catcaactgg cgaaacagag cctggtcctc ctcgctaagt gttgttttct ttttcattta
    14341 tctgtctcta tttctaattt tcctacagtt tacccgactc gtagcacaat cgatcgtttt
    14401 aaacgtattt ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa
    14461 actagccgcc gaaattattg cgatcatgcc ctggaggaat acgtggataa aattttcgtt
    14521 gatgaagcag taaatgagct gcaaaccatt caggacatgt tgcgctggtc ggtaagccgc
    14581 ttcagcgcgg caaatatctg gtacggtcac ggcaccgata acccgtggga tgaagccgta
    14641 cagctggtgt taccatcgct ctacctgccg ctggatatcc cggaagatat gcgcaccgcg
    14701 cgtctgacct ccagcgaaaa acaccgtatt gttgaacgcg tgatccgccg cgtcaacgag
    14761 cgcattccgg tggcttacct gaccaacaaa gcctggttct gcggtcatga attttacgtc
    14821 gatgaacgcg tgctggtgcc gcgctcgccg attggtgaac tgatcaacaa taaatttgcc
    14881 ggactcatta gcaagcaacc gcagcatatt ttagatatgt gtaccggtag cggctgcatc
    14941 gccattgcct gtgcttatgc cttcccggaa gcggaagtcg acgcggtaga tatctctccc
    15001 gacgcgctgg cggttgccga acagaacatc gaagaacacg gtctgatcca caacgtcatt
    15061 ccgattcgtt ccgatctgtt ccgtgatttg ccgaaagtgc agtacgatct gattgtcact
    15121 aacccgccgt atgtcgacgc ggaagatatg tccgacctgc caaacgaata ccgtcacgag
    15181 ccggaactgg gcctggcatc tggcaccgac ggccttaaac tgacgcgtcg cattcttgga
    15241 aacgcggcag attaccttgc tgatgatggc gtgttgattt gtgaagtcgg caacagcatg
    15301 gtacatctta tggaacaata tccggatgtt ccgttcacct ggctggagtt tgataacggc
    15361 ggcgatggtg tgtttatgct caccaaagag cagctgctgg cggcccgtga atatttcgcg
    15421 atttataaag attaagtaaa cacgcaaaca caacaataac ggagccgtga tggctggaaa
    15481 cacaattgga caactctttc gcgtaaccac cttcggcgaa tcgcatgggc tggcgctcgg
    15541 ctgcatcgtc gatggtgttc cgccaggcat tccgctgacg gaagcggacc tgcaacatga
    15601 cctcgaccgt cgtcgccctg ggacatcgcg ctataccacc cagcgccgcg agccggatca
    15661 ggtcaaaatt ctctccggtg tttttgaagg cgttactact ggcaccagca ttggcttgtt
    15721 gatcgaaaac actgaccagc gctctcagga ttacagcgca attaaagacg ttttccgccc
    15781 aggccatgct gattacacct acgaacaaaa atacggtctg cgcgattatc gcggcggtgg
    15841 acgttcttcc gcccgcgaaa ccgccatgcg cgtggcggca ggggcgattg ccaaaaaata
    15901 tctcgccgag aaatttggta ttgaaatccg cggctgcttg acccagatgg gcgacattcc
    15961 gctggaaatc aaagactggt cgctggttga acaaaatccg ttcttctgcc cggacccgga
    16021 caaaatcgac gcgttagatg aactgatgcg cgcgctgaaa aaagagggcg actccatcgg
    16081 cgcgaaagtc accgttgttg ccagtggtgt ccccgccgga cttggcgagc cggtctttga
    16141 ccgcctggat gccgacatcg cccatgcgct gatgagcatc aacgcggtga aaggcgtgga
    16201 aattggcgac ggttttgacg tagtggcgct gcgcggcagc cagaaccgcg acgaaatcac
    16261 caaagacggt ttccagagca accatgcggg cggcattctc ggcggtatca gcagcgggca
    16321 gcaaatcatt gctcatatgg cgctgaaacc gacctccagc attaccgtgc cggggcgcac
    16381 tattaaccgc tttggcgaag aagttgagat gatcaccaaa ggccgtcacg atccctgtgt
    16441 cgggatccgt gcagtgccga tcgctgaagc gatgctggcg atcgttttaa tggatcacct
    16501 gttacggcag cgggcgcaaa atgccgatgt gaagactgat attccacgct ggtaaaaaat
    16561 gaacaaaacc gcgattgcgc tgctggctct gcttgccagt agcgctagcc tggcagcgac
    16621 gccgtggcaa aaaataaccc aacctgtgcc gggtagcgca caatcgatag gcagtttttc
    16681 taatggctgt attgtcggcg ctgacacgtt gccgatacag tccgaacatt atcaggtcat
    16741 gcgtaccgat cagcgtcgct atttcggtca cccggatctg gtgatgttta tccagcgtct
    16801 gagtagccag gtgagcaatc tgggcatggg tacggtgctg attggcgata tggggatgcc
    16861 cgctggtggg cgtttcaacg gcggtcatgc cagccaccag accggactgg atgtcgatat
    16921 ctttctgcaa ctgccgaaaa ctcgctggac ctccgcgcag ctcttgcgcc cgcaagcact
    16981 ggacttagtt tcccgcgacg gtaaacacgt tgtctccacg ctgtggaagc cagaaatttt
    17041 cagcttgatc aaactcgctg cccaggacaa agacgtcacg cgcatttttg ttaatccggc
    17101 gattaaacaa caactttgcc ttgatgcggg caccgatcgc gactggttgc gcaaagtgcg
    17161 accctggttc cagcatcgcg cgcatatgca tgtacgatta cgttgccctg ccgatagtct
    17221 ggagtgtgaa gatcaacctt taccgccatc aggcgatggt tgcggggcag aactgcaaag
    17281 ctggtttgca cctctaaaac cgggaacaac aaagcctgag aagaagacac cgcctccgtt
    17341 gccgccttcc tgccaggcgc tactggatga gcacgtgatc taatggaaac gtttaatagc
    17401 ctgtttatgg tttccccgct gttgctggga gttctctttt ttgtcgccat gctggcggga
    17461 tttatcgact cgattgccgg tggcggtggg ttactcacca ttccggcatt gatggctgcg
    17521 ggaatgtctc ccgctaacgc gctggcaacc aataaactgc aagcctgcgg cggctctatt
    17581 tccgccacta tctactttat tcgccgcaaa gtggttagtt taagcgatca gaaactcaat
    17641 attgccatga cctttgtcgg ctcaatgagc ggcgcactgt tggtacagta cgttcaggct
    17701 gatgtcttac ggcagatttt gcccattctg gtgatttgta tcggcctcta tttcttgctg
    17761 atgccaaagc tgggtgaaga agatcgccag cgccggatgt acggtttacc cttcgcgtta
    17821 attgccggtg gctgcgtcgg tttttatgac ggattctttg gcccggctgc cggatcgttt
    17881 tacgcgctgg ccttcgttac gctgtgcgga ttcaacctcg ccaaagccac ggctcacgcc
    17941 aaattactca acgcaacgtc aaacattggc ggtctgctgc tgtttattct cggcggcaaa
    18001 gtgatttggg cgacgggctt tgtcatgctg gtggggcagt tcctcggcgc gcgcatgggt
    18061 tcacggctgg tgttgagcaa aggacaaaag ctgatccgcc cgatgattgt tatcgtctcg
    18121 gcggtgatga gtgccaaatt actttacgat agccacggac aggagatcct ccactggttg
    18181 gggatgaact aatgaacagt acacaccact atgagcagtt gattgaaatt tttaatagct
    18241 gctttgccga tgaatttaat acccgtctga ttaaaggcga cgacgaaccg atctatcttc
    18301 ctgctgatgc ggaagtaccg tataaccgaa ttgtctttgc ccacggcttt tatgccagcg
    18361 caattcatga gatttcgctc tggtgtattg ccggaaaagc gcgccgtgag ctggttgatt
    18421 tcggctactg gtattgcccg gatgggcgtg atgcccaaac gcaaagccag tttgaagatg
    18481 ttgaagtgaa gccgcaggca ctcgactggt tgttctgtgt ggcggcggga tatccgttta
    18541 acgtcagttg cgacaatctg gaaggcgact tcgaaccgga tcgtgttgtg tttcaacggc
    18601 gggtacatgc gcaggtgatg gattatctga caaatggcat tcccgagcgt ccggcacgct
    18661 tcattaaagc actacaaaat tattatcaca cgccggaact tacggcggaa cagttcccgt
    18721 ggccggaagc gctcaactga agccggtgcc acgttttaca atgaggaaag aagatgatcg
    18781 cggagtttga atcacgcatt ctggcattaa tcgacggcat ggttgaccat gccagtgatg
    18841 atgagttgtt tgccagtggg tatttgcgtg gccacctgac attagccatc gcagaactgg
    18901 aaagtggtga tgaccactcc gctcaggcgg tgcatacgac cgttagccag agtctggaaa
    18961 aagccattgg tgccggtgaa ttgtcgccgc gtgaccaggc gctggtgacc gatatgtggg
    19021 aaaacctgtt tcagcaggcg tcacagcagt aagttgcggc gtctggagca ccatgcctga
    19081 tgcgacgctt atcgcgtctt atcaggccta cacgtcacac gtaggtcgga taaggcgttc
    19141 acgccgcatc cggcaaacac tgcgagagca gattaccccg ccttaaccgc tttacccttc
    19201 aacaatttcc gcacccataa ccgattcggg tttaacgccg tcagcgtact ggcatccatc
    19261 ggaatcggtt cgtcgctcat ctgcgccgcc agaatctcgg cacacagcgg ggcagaacac
    19321 aaaccgcgag aacctaaagc cgcaaacata aagagatcgt caaaaaccgg cgcgcttacc
    19381 gcctcatctt tctgctccgc caacgacgca tattccacga gtgttgcctc ataatcggga
    19441 acattgccta ccattggcag atgatcgcgg gtggcacaac gcacaccgca gcgcgcctct
    19501 ttatcactga catcaacctc ttttgcccac tgtgcctgcg ggaaacaatc aatcaaccgc
    19561 tggcgattct gctgctgatc gtcctcactg tacgccgtat cttcgctgcc gcgatgataa
    19621 ctggcaccaa tacaatgatg ttgattcgcc ggattttgtg gcgtgagata accgtcatag
    19681 cacagcacct gcttcagctc tgccagttcc ggcgttgtcg gaatatggct gacctgcccg
    19741 gcaaccgaat acaccgggag agtcgacgtt tggctgaaac ggctgatttg atgcccgttc
    19801 gccagtacca ctacgctgtg tgttgcttgc tgatctcctg caaaattcaa caaccaacag
    19861 tcatccttac gggataaatt ctgtaactga tattgataat aaatctgcaa accctgctgt
    19921 tgcgccagtt ccagcacatt acgggtcagt tctgctgggc acagccaacc accttgcgga
    19981 taagtaatgc cgctgcaatt tgtcgcaacg cccgtaattt gttcaaccgc atttgcctca
    20041 acggctacag ccagttctgc gggtaaatcc attgacaaca tctgtgcgat tttatgctgg
    20101 cttttctcat cccagcctaa ctgcgtgacg ccgcaccagt catgatcaaa tttaacgggt
    20161 aattggtcgt aaaaccgacg agcaaaagta aacgcattag agaaaaagcg gtttagcgcc
    20221 tcatcgtgtt tgcttaataa cggatacagc gccccctggc gattgccgga agcacccagc
    20281 gcgggggctt catccgcgca ataaagcgct acctgccagc cgcgccgtaa taacgccagc
    20341 gacaacaacg cgctggcaat accaccgccg ataatcgcta cttcccgttt gctgctgccc
    20401 gtacgattaa accacggcgt ggagcagggg agtggtaatg tctgttccat caccccacaa
    20461 agcatttccc gtttgcgccc aaagccctta cgtttttgca tcgtgaatcc ggcttcctgc
    20521 aaaccgcggc ggacaaaacc ggcagacgta aatgtcgcca gcgtgccgcc tggacgcgcc
    20581 aaccttgcca tggcgttaaa cagattttgc gtccacatat ccgggttttt agctggcgca
    20641 aagccgtcca gaaaccaggc atctactttt tgatttagcg aatcgtccag ttggctggtc
    20701 agttcgttaa tatcgccaaa ccataaatcc agcgtcacgc ggccttcatt gagcaataaa
    20761 cgatggcaac cgggcaaggg cattggccac tgcgcctgaa gttgttctgc ccacggagcc
    20821 agttccggcc agtgttgatg cgctaaggct aaatccgcac gggtgagggg aaatttctca
    20881 aaactaatga aatgtaagcg ttgtaattgc gcttgcggat gcgcttcgcg aaactgatca
    20941 aatgcctgcc atagcgtcag gaagtttaat ccggtgccga agccgctctc tgctaccaca
    21001 aacagaggat gtggatgctc aggaaagcgt acctctaatt ggttgcctcc cagaaaaaca
    21061 taacgcgtct cttccagccc gttatcgttg gaaaaataga catcgtcaaa atctcgggaa
    21121 acaggtgtac cctcagcatt aaattcgagg ttggcaggtt gtatggagta gtgtttcacg
    21181 taagttactc gtcttacagg cggtggctcg atcttagcga tgtgtgtaag gctgcgcaaa
    21241 tttctctatt aaatggctga tcggacttgt tcggcgtaca agtgtacgct attgtgcatt
    21301 cgaaacttac tctatgtgcg acttacagag gtattgaatg aaacgtgcag tgattactgg
    21361 cctgggcatt gtttccagca tcggtaataa ccagcaggaa gtcctggcat ctctgcgtga
    21421 aggacgttca gggatcactt tctctcagga gctgaaggat tccggcatgc gtagccacgt
    21481 ctggggcaac gtaaaactgg ataccactgg cctcattgac cgcaaagttg tgcgctttat
    21541 gagcgacgca tccatttatg cattcctttc tatggagcag gcaatcgcag atgcgggcct
    21601 ttctccggaa gcttaccaga ataacccgcg cgttggcctg attgcaggtt ctggcggcgg
    21661 ttccccgcgt ttccaggtat tcggcgctga cgcaatgcgc ggcccgcgcg gcctgaaagc
    21721 ggttggcccg tatgtggtca ccaaagcgat ggcatccggc gtttctgcct gcctcgccac
    21781 gccgtttaaa attcatggcg ttaactactc catcagctcc gcgtgtgcga cttccgcaca
    21841 ctgtatcggt aacgcagtag agcagatcca actgggcaaa caggacatcg tgtttgctgg
    21901 cggcggcgaa gagctgtgct gggaaatggc ctgcgaattc gacgcgatgg gtgcgctgtc
    21961 tactaaatac aacgacaccc cggaaaaagc ctcccgtact tacgacgctc accgtgacgg
    22021 tttcgttatc gctggcggcg gcggtatggt agtggttgaa gagctggaac acgcgctggc
    22081 gcgtggtgct cacatctatg ctgaaatcgt tggctacggc gcaacctctg atggtgcaga
    22141 catggttgct ccgtctggcg aaggcgcagt acgttgtatg cagatggcga tgcatggcgt
    22201 tgatacccca atcgattacc tgaactctca cggtacttcg actccggttg gcgacgtgaa
    22261 agagctggca gctatccgtg aagtgttcgg cgataagagc ccggcgattt ctgcaaccaa
    22321 agccatgacc ggtcactcac tgggcgctgc gggtgtgcag gaagctatct actctctgct
    22381 gatgctggaa cacggcttta tcgccccgag catcaacatt gaagagctgg acgagcaggc
    22441 tgcgggtctg aacatcgtga ccgaaacgac cgatcgcgaa ctgaccactg ttatgtctaa
    22501 cagcttcggc ttcggcggca ccaacgccac gctggtaatg cgcaagctga aagattaatt
    22561 cgcagtaggc cggagtaggt cggataagac gcgccagcgt cgcatccgac gttacgcgcc
    22621 aatgcggcct ccggcactaa cgcaaaaggg aacctgatgg ttcccttttt cacatcattg
    22681 acaatcgcca ccagttccag gcaaacttcc cgctttgtcg atttccttct gaaaagacgt
    22741 acgcgttaaa tcctgccaac gcaccgtaac cctgaaacca gagagatgag acggggatac
    22801 tcctcgcctt gcgctgcatt ctggagtaat gcatgactgc tgtaagccaa accgaaacac
    22861 gatcttctgc caatttttcg ctcttccgca tcgcttttgc ggtttttctc acctacatga
    22921 ccgtagggct gccgttgccg gttatcccgc tgtttgttca tcatgaactg ggctatggca
    22981 ataccatggt cggcattgcc gtcgggattc agtttctggc tacggtgctg acgcgtggct
    23041 atgccgggcg actggccgat caatatggcg caaaacgttc ggcgcttcag gggatgttag
    23101 cttgtggtct ggctggcggc gcgttgctgc tggcggcgat tttgcctgtc tccgcaccgt
    23161 tcaaatttgc cctgttggtc atcgggcgtt tgattcttgg gtttggtgaa agccagttac
    23221 tgacaggcgc tctgacctgg gggttaggca tcgtagggcc aaaacactct ggcaaagtga
    23281 tgtcatggaa tggaatggcg atttacggtg ccctcgctgt tggtgctccg cttggcctgt
    23341 tgattcatag ccattacggt tttgccgcac tggcgatcac cacaatggta ttacccttac
    23401 tggcgtgggc ctgtaacggc acagtgcgca aagtaccggc cctggcggga gaacgtccat
    23461 cgctgtggag cgttgtcggg tttatctgga aaccagggtt agggctggca ctacaaggcg
    23521 ttggttttgc ggttatcggg actttcgttt cgctctactt tgccagcaaa ggatgggcga
    23581 tggcgggctt tactcttacc gagtttggcg gcgcatttgt cgtgatgcgc gtcatgtttg
    23641 gctggatgcc ggaccgtttt ggcggcgtga aagtggcgat tgtctctctg cttgtagaaa
    23701 cggtgggctt gttgctgctc tggcaagccc caggtgcgtg ggtcgcatta gcgggcgcgg
    23761 cgttaaccgg agccggatgt tcgcttatct ttcctgcgct gggcgtggag gtggttaaac
    23821 gcgtcccctc acaagttcgc ggcaccgcac tgggcggtta cgccgcgttt caggatatcg
    23881 ccctcggcgt ctccgggccg ctggcgggaa tgctggcgac cacgtttggt tactcttcgg
    23941 tatttcttgc cggggcgatc tctgcggtgc tgggaattat tgtcacgata ctgtcatttc
    24001 gtcggggcta acgaaccagc cagaccagca tcaggataat cgcgactaac agtatccaca
    24061 gcgcgggacg cgttgccata ttttgcaacg tgtccatcat cggtggaaaa ggattaagca
    24121 gcggttgcat atcacgcgga tcaatccctt taacccgccg ttgataaagc tgattaagaa
    24181 tatcctcggc ctgggcagaa gaaagcgatg attgcgcaga aagaccatat ttttgctgga
    24241 tatacgccga taacgctgcc agttcactgg catctaaagg ttgttttagc gccatctgta
    24301 gtgattccag cgtcggcgta ttttgctggc ttaacgtctg gcgcgcctgc aaccaggtca
    24361 ccagatggtt aaacagtttc gctggaatta actcgccatc gttcaccccg gaaagttcca
    24421 gcatcgattg ccagatctgt ttgctgggtt cccccgttgc cgccgcaagt ttggtcacca
    24481 gctgttttag cgcattgtgc tccgccggta ataaaggacg gtcggtcgcc tcgcgctgct
    24541 gtggttgcgg aataaccatc ttcccttcct gcaacagggt gagaatggtt tttaattgct
    24601 ccggtgagag ctgattcagc ggcgtctgac caaagttatg acggatataa tccgtgaccg
    24661 cctgacgatt attccccaga cgtaaatact cccctaattg cgctaaaagc tggcgggcag
    24721 aatggctttt ttgcgcggcc agcagacgtt gcgccagatt atgctcagcg gcagggaagt
    24781 gacgcgaaag cagcggtgaa tctcccgaca gaccaatatc gtgcctgata cccgcccaca
    24841 gttctgctct ttgttgttgc gtcagtgagg tcactttcgt cattaagctt tccagcgaag
    24901 tacgttgctg actggataaa ggctgattgc ccgcgccaga cggcaggtta tctccctgac
    24961 ctggtggttg cccaggagga gggccggaaa taggttgtat cattacgtat ccttatacct
    25021 gaaatcttcg caagtatgcc tggccgcgag attatggcac acttgtccgg ttaactctcg
    25081 tctcatacag gtaacacaaa cgtgaaaatc cttgttgatg aaaatatgcc ttatgcccgc
    25141 gacttattta gccgtttggg tgaggtgacc gcggttcccg ggcgtccaat ccccgtcgct
    25201 caactggcag acgcggatgc gctgatggtg cgttcggtca cgaaagtgaa tgaatctttg
    25261 ctggcaggaa aacccattaa atttgttggc actgccactg cggggaccga ccatgtcgat
    25321 gaagcatggt tgaagcaggc gggaattggt ttttccgctg cacctggctg taatgcgatt
    25381 gcggtggtgg aatatgtttt ctcctcacta ctgatgcttg ccgaacgcga tggattttca
    25441 ctgcacgacc gtacggtggg gatcgtgggc gttggtaacg ttggacgtcg attgcaggcg
    25501 cgactggaag cgttagggat taaaacattg ctttgcgatc cgcctcgcgc tgaccgtggg
    25561 gatgagggtg atttccgctc gctggatgaa ttagttcagc atgcggatat tctgactttc
    25621 catacgccac tcttcaaaga tgggccgtac aaaacgctgc atctggcgga tgaaaaactg
    25681 atccgtagcc tgaagccggg agcgattctg attaacgcct gccgtggcgc agtcgtcgat
    25741 aatactgcgt tgctgacctg cctgaatgaa ggccagaagt taagcgtagt gctggatgtc
    25801 tgggaaggcg aaccggaact taacgtggag ttgctgacaa aagtggatat cggcacgccg
    25861 catatcgcag gttataccct ggaaggtaaa gcacgcggta ctacgcaagt gtttgaagcc
    25921 tatagcaagt ttattgggca tgaacagcac gttgcgctgg atacattact gcccgcgcca
    25981 gagtttggtc gcattacgct gcatggcccg ctcgatcaac cgacgctgaa aaggctggtg
    26041 catttggtgt atgatgtgcg ccgcgatgac gcaccgctgc gtaaagtcgc cgggataccg
    26101 ggtgagttcg ataaactgcg caaaaactat cttgagcgcc gtgaatggtc atctctgtat
    26161 gtaatttgtg atgacgccag tgcggcatca ttgctgtgta aactgggttt taacgccgtt
    26221 catcatccgg cacgttaatc tcttcttcat gctctctgct gtaacattgg cagggagctt
    26281 tgctatttct ggagtaaacc accatgtctg aaggctggaa cattgccgtc ctgggcgcaa
    26341 ctggcgctgt gggcgaagcc ctgcttgaaa cgctggctga acgtcagttc ccggttgggg
    26401 aaatttatgc actggcacgt aacgaaagcg caggcgaaca actgcgcttt ggtggtaaaa
    26461 caatcactgt gcaggatgcc gctgaattcg actggacgca ggcgcagctg gcattttttg
    26521 tcgcaggcaa agaagctacc gctgcctggg ttgaagaagc gaccaactca ggttgcctgg
    26581 tgatcgacag cagcggactg tttgctctcg aacccgacgt accgctggtg gtgccggaag
    26641 taaacccgtt tgtactgacc gattaccgca accggaatgt catcgccgta ccagacagtc
    26701 tgaccagcca gctgctggcg gcactgaaac cgttaatcga tcagggcggt ttgtcgcgta
    26761 tcagtgttac cagcctgatt tcagcctccg cccagggcaa aaaagcggtc gatgcgttag
    26821 cggggcagag tgcgaaattg ctcaacggca ttccgattga cgaagaagat ttcttcggtc
    26881 gtcaactggc gttcaatatg ctgccgttgt tgccggatag cgaaggtagc gtgcgtgaag
    26941 aacgtcgtat cgttgacgaa gtacgcaaaa tcctgcagga cgaagggctg atgatttcgg
    27001 caagcgtcgt ccaggcaccg gtattctacg gtcatgccca gatggtcaac tttgaagcac
    27061 tgcgtccgct ggcggcagaa gaagcgcgtg atgcgtttgc tcagggcgaa gatattgtgc
    27121 tctctgaaga gaacgaattc ccgactcagg taggggatgc ttcgggtacg ccgcatcttt
    27181 ctgttggctg cgtgcgtaat gactacggta tgccggagca agtccagttc tggtcggtgg
    27241 ccgataacgt tcgctttggc ggcgcgctga tggcagtaaa aatcgccgag aaactggtgc
    27301 aggagtatct gtactaatgt ccgaccagca acaactgcca gtttataaaa ttgcgctggg
    27361 cattgagtac gacggcagta agtattacgg ctggcaacgg cagaatgaag tccgcagtgt
    27421 gcaggagaag ctggaaaagg cgctctccca ggtggcgaac gaacccatca ccgtcttctg
    27481 cgccgggcgt actgacgcag gggtacacgg taccgggcag gtggtgcatt tcgaaacaac
    27541 cgcgcagcgc aaagacgcgg cgtggacctt gggcgtaaat gcgaatttac ctggtgacat
    27601 cgccgtgcgt tgggttaaag ctgtacctga tgattttcat gcccgattta gcgccacggc
    27661 tcgccgttat cgctacatca tctacaatca tcggctgcgc ccggcggtat tgagtaaagg
    27721 ggtaacccat ttttacgaac cgctggacgc tgaacggatg catcgggctg cgcaatgctt
    27781 gctgggcgag aatgatttca cctcgttccg tgcggtgcag tgccagtccc gaaccccgtg
    27841 gcgcaacgtt atgcatatta atgtcacgcg tcacggtcct tatgtggtgg tagatatcaa
    27901 agcgaatgcc tttgtacatc atatggtcag gaatattgtc ggcagcctga tggaagtcgg
    27961 tgcccacaac cagccggaga gatggatagc agagttgctg gcggcaaagg acagaacgct
    28021 ggcggcagca acggcaaaag cggaagggct gtatctggtc gcggtggatt accctgaccg
    28081 gtatgatctt ccaaaaccgc caatgggccc gctatttctg gcggactaac gagaataatg
    28141 ctcggaacat ttcaggctgt agccaacgta gttacagcct gaaagatgac gagtacaagg
    28201 cataggcaaa tatggacctg atttatttcc tcatcgattt tatcctgcac attgacgtgc
    28261 atctggcaga gctggtcgcg gagtacggcg tctgggttta tgccattctg tttttgattc
    28321 tgttctgcga aacggggctg gtggtaacgc cattcctgcc tggtgattcg ctgctgtttg
    28381 ttgctggtgc attggcgtcg ctggaaacta acgatctcaa tgtccatatg atggtggtgc
    28441 ttatgttgat tgccgctatt gtcggtgatg cagtgaacta taccattgga cggttatttg
    28501 gtgacaaact gttcagtaac ccggattcga aaatctttcg tcgcagttat cttgataaga
    28561 ctcatcagtt ttacgagaga catggcggca aaacgattat tctcgcccgt tttgtaccga
    28621 tcgtcagaac gttcgcgccg tttgttgcgg gaatgggcca catgtcgtac cgtcatttcg
    28681 ccgcttataa cgtgatcggc gcactgttgt gggtactgat ttttacctac gcgggttatt
    28741 tcttcggcac gctccccttc attcagagca accttaagct gatgattgtt ggcattattt
    28801 ttgtttcaat attgcctggg gttttcgaat ttattcgtca caaacgcgct gcggcacgcg
    28861 ccgcaaaata aaaagtaact ccgcggttcg accacttttt tatccaaagt ttcgggctgt
    28921 tatgttttaa tgtgcaacat tcatggtctg ttgggggcaa aaatggcatt atgcgtcccc
    28981 aaagataaaa ctggcatcga accaggttca gacagaaagg tccctaatga gctggattga
    29041 acgaattaaa agcaacatta ctcccacccg caaggcgagc attcctgaag gggtgtggac
    29101 taagtgtgat agctgcggtc aggttttata ccgcgctgag ctggaacgta atcttgaggt
    29161 ctgtccgaag tgtgaccatc acatgcgtat gacagcgcgt aatcgcctgc atagcctgtt
    29221 agatgaagga agccttgtgg agctgggtag cgagcttgag ccgaaagatg tgctgaagtt
    29281 tcgtgactcc aagaagtata aagaccgtct ggcttctgcg cagaaagaaa ccggcgaaaa
    29341 agatgcgctg gtggtgatga aaggcactct gtatggaatg ccggttgtcg ctgcggcatt
    29401 cgagttcgcc tttatgggcg gttcaatggg gtctgttgtg ggtgcacgtt tcgtgcgtgc
    29461 cgttgagcag gcgctggaag ataactgccc gctgatctgc ttctccgcct ctggtggcgc
    29521 acgtatgcag gaagcactga tgtcgctgat gcagatggcg aaaacctctg cggcactggc
    29581 aaaaatgcag gagcgcggct tgccgtacat ctccgtgctg accgacccga cgatgggcgg
    29641 tgtttctgca agtttcgcca tgctgggcga tctcaacatc gctgaaccga aagcgttaat
    29701 tggctttgcc ggtccgcgtg ttatcgaaca gaccgttcgc gaaaaactgc cgccaggatt
    29761 ccagcgcagt gaattcctga tcgagaaagg cgccatcgac atgatcgtcc atcgtccgga
    29821 aatgcgcctg aaactggcga gcattctggc gaagttgatg aatctgccag cgccgaatcc
    29881 tgaagcgccg cgtgaaggcg tagtggtacc cccggtaccg gatcaggaac ctgaggcctg
    29941 ataactgata agggcagggc cactggctct gcccttttgc tattctcacc gtaacgaatc
    30001 agcggatacc atgattatca aacgcactcc tcaagccgcg tcgcctctgg cttcgtggct
    30061 ttcttatctg gaaaacctgc acagtaaaac tatcgatctc ggccttgagc gcgtgagcca
    30121 ggtcgcggcg cgtcttggcg tcctgaaacc agcgccattt gtgtttaccg ttgcgggtac
    30181 gaatggcaaa ggcaccacct gccgtacgct ggagtcgatt ctgatggcgg cagggtacaa
    30241 agtgggcgtc tacagttcac cgcatctggt gcgttatacc gagcgcgtac gtgtgcaggg
    30301 gcaggaattg ccggaatcgg cccacaccgc ctcttttgcg gagattgaat cggcacgcgg
    30361 tgatatttcc ctgacctatt tcgagtacgg tacgctgtcg gcgttgtggc tgttcaaaca
    30421 ggcacaactt gacgtagtga ttctggaagt agggctgggc ggtcgtctgg acgcaaccaa
    30481 tattgtcgat gccgatgtgg ctgtagtaac cagcattgcg ctggatcata ccgactggct
    30541 gggaccagat cgcgaaagta ttggtcgcga gaaagcaggc atctttcgca gcgcaaaacc
    30601 ggcaattgtc ggtgagccgg aaatgccttc taccattgct gatgtggcgc aggaaaaagg
    30661 tgcactgtta caacgtcggg gcgttgagtg gaactattcc gtcaccgatc atgactgggc
    30721 gtttagcgat gctcacggca cgctggaaaa tctgccgttg ccgcttgtcc cgcaaccgaa
    30781 tgccgcaaca gcgctggcgg cactgcgtgc cagcgggctg gaagtcagtg aaaatgccat
    30841 tcgcgacggg attgccagcg caattttgcc gggacgtttc cagattgtga gcgagtcgcc
    30901 acgcgttatt tttgatgtcg cgcataatcc acatgcggcg gaatatctca ccgggcgtat
    30961 gaaagcgcta ccgaaaaacg ggcgcgtgct ggcggttatc ggtatgctac atgataaaga
    31021 tattgccgga actctggcct ggttgaaaag cgtggttgat gactggtatt gtgcgccact
    31081 ggaagggccg cgcggtgcca cggcagaaca actgcttgag catttgggta acggcaaatc
    31141 atttgatagc gttgcgcagg catgggatgc cgcaatggcg gacgctaaag cggaagacac
    31201 cgtgctggtg tgtggttctt tccacacggt cgcacatgtc atggaagtga ttgacgcgag
    31261 gagaagcggt ggcaagtaag tttcagaatc ggttagtggg cacgatcgtg ctggtggcgc
    31321 tgggggtgat tgtacttcca gggctgctgg acgggcagaa aaaacattat caggatgagt
    31381 ttgcggctat cccgctggtt ccaaaagcgg gcgatcgcga tgagcctgat atgatgccag
    31441 ccgccaccca ggcgttgccg acgcagccgc cggaaggcgc agcggaagag gtgcgggcag
    31501 gtgatgccgc agcaccgtcg ctcgatccgg ccactattgc agccaataac accgagtttg
    31561 aaccggaacc tgcaccggtc gccccaccga agccgaaacc ggtggagccg cctaaaccga
    31621 aggttgaagc accacctgcg ccgaagccag aaccgaagcc ggtcgtggaa gaaaaagcag
    31681 cgccaacggg taaagcctat gttgtgcaac tgggtgcgct gaaaaatgcc gataaagtga
    31741 atgagattgt cggtaagctg cgcggtgccg gttatcgggt ttatacgtcg ccatccacgc
    31801 cagtgcaggg taaaattacc cgtattctgg ttgggccgga tgcctcgaaa gataagctga
    31861 aaggttcgct gggtgagttg aagcaacttt ctggcttaag tggcgtggta atgggctata
    31921 cgccgaatta atacagtctt gcctgatgcg acgctggcgc gtcttatcag gcctgggcag
    31981 tgttagaacc gtaggtcgga taaggcgttt acgccgcatc cgacacgcat tgcacgatgc
    32041 cgcaaaggca taaaaagtcg atggcgttga atattttttc agcgccattt ttattgatgc
    32101 gtgggaagga aatccctacg caaacgtttt ctttttctgt tagaatgcgc cccgaacagg
    32161 atgacagggc gtaaaatcgt gggacacata tggtctggat tgattacgcc ataatcgcgg
    32221 tgattgcttt ttcctctctg gttagcctga tccgcggctt tgttcgtgaa gcgttatcgc
    32281 tggtgacatg gggttgtgct ttctttgttg ccagtcatta ctacacttac ctgtcagtct
    32341 ggtttacggg ctttgaagac gaactggttc gaaatggaat tgccatcgcg gtactgttta
    32401 tcgctaccct gattgttggt gctatcgtga acttcgtgat aggccagttg gtggagaaaa
    32461 cggggttgtc aggcaccgat cgggtgctgg gcgtctgttt cggtgcgttg cgcggtgtgt
    32521 tgattgttgc cgccattctc ttctttctcg actcctttac cggggtgtcg aaaagcgaag
    32581 actggagcaa atcacagctg atcccgcagt tcagttttat catcagatgg ttttttgatt
    32641 atctgcaaag ctcgtcaagt ttcttgccca gagcgtaagt gctctgagat gtggcttaac
    32701 gaggaaaaag acgtatgtgc ggtattgtcg gtatcgccgg tgttatgccg gttaaccagt
    32761 cgatttatga tgccttaacg gtgcttcagc atcgcggtca ggatgccgcc ggcatcatca
    32821 ccatagatgc caataactgc ttccgtttgc gtaaagcgaa cgggctggtg agcgatgtat
    32881 ttgaagctcg ccatatgcag cgtttgcagg gcaatatggg cattggtcat gtgcgttacc
    32941 ctacggctgg cagctccagc gcctctgaag cgcagccgtt ttacgttaac tccccgtatg
    33001 gcattacgct tgcccacaac ggcaatctga ccaacgctca cgagttgcgt aaaaaactgt
    33061 ttgaagaaaa acgccgccac atcaacacca cttccgactc ggaaattctg cttaatatct
    33121 tcgctagcga gctggacaac ttccgccact acccgctgga agccgacaat attttcgctg
    33181 ccattgctgc cacaaaccgc ttaatccgcg gcgcatatgc ctgtgtggcg atgattatcg
    33241 gccacggtat ggttgctttc cgcgatccaa acgggattcg tccgctggta ctgggaaaac
    33301 gtgatattga cgagaaccgt acagaatata tggtcgcttc cgaaagcgta gcgctcgata
    33361 cgctgggctt tgatttcctg cgtgacgtcg cgccgggcga agcgatttac atcactgaag
    33421 aagggcagtt gtttacccgt caatgtgctg acaatccggt cagcaatccg tgcctgtttg
    33481 agtatgtata ctttgctcgc ccggactcgt tcatcgacaa aatttccgtt tacagcgcgc
    33541 gtgtgaatat gggcacgaaa ctgggcgaga aaattgcccg cgaatgggaa gatctggata
    33601 tcgacgtggt gatcccgatc ccagaaacct cgtgtgatat cgcgctggaa attgctcgta
    33661 ttctgggcaa accgtaccgc cagggcttcg ttaaaaaccg ctatgttggc cgcaccttta
    33721 tcatgccggg ccagcagctg cgtcgtaagt ccgtgcgccg taaactgaat gccaaccgcg
    33781 ccgagttccg cgataaaaac gtcctgctgg tcgacgactc catcgtccgt ggcaccactt
    33841 ctgagcagat tatcgagatg gcacgcgaag ccggagcgaa gaaagtgtac ctcgcttctg
    33901 cggcaccgga aattcgcttc ccgaacgttt atggtattga tatgccgagc gccacggaac
    33961 tgatcgctca cggtcgcgaa gttgatgaaa ttcgccagat catcggtgct gacgggttga
    34021 ttttccagga tctgaacgat ctgatcgacg ccgttcgcgc tgaaaatccg gatatccagc
    34081 agtttgaatg ctcggtgttc aacggcgtct acgtcaccaa agatgttgat cagggctacc
    34141 tcgatttcct cgatacgtta cgtaatgatg acgccaaagc agtgcaacgt cagaacgaag
    34201 tggaaaatct cgaaatgcat aacgaaggat gatgccttcg ctgagggtgc cggtctggca
    34261 ccctgacttg caactcccgc cgaaatacta caaagtctgc ctgcaagtct gacagggcaa
    34321 ctatttatga aacgactcat tgtaggcatc agcggtgcca gcggcgcgat ttatggcgtg
    34381 cgcttattac aggttctgcg cgatgtcaca gatatcgaaa cgcatctggt gatgagccag
    34441 gcggcgcgcc agaccttatc cctcgaaacg gatttttccc tgcgcgaagt gcaggcatta
    34501 gctgatgtca cgcacgatgc gcgcgatatt tccgccagca tctcttccgg ttctttccag
    34561 acgctgggga tggtgatttt accctgttca atcaaaaccc tttccggcat tgtccatagc
    34621 tatactgatg gcttactgac ccgtgcggca gatgtggtgc tgaaagagcg tcgcccgttg
    34681 gtgctctgcg tgcgtgaaac accattgcac ttaggccatc tgcgtttaat gactcaggcg
    34741 gcagaaatcg gtgcggtgat tatgcctccc gttccggcgt tttatcatcg cccgcaatcc
    34801 cttgatgatg tgataaatca gacggttaat cgtgttcttg accagtttgc aataaccctt
    34861 cctgaagatc tctttgcccg ctggcagggc gcataactcc gctgttgccc tgtttcaggg
    34921 caattttgca accgcgatca aatcctcgac attttgtttc tgccattcaa tcgaaacgct
    34981 gcgattcaac cgctatacct gctatcttca acttcaggac aataatgcaa cgtcttatta
    35041 acatatttaa cgttgaatgt tactgttgtc gtcaagatgg cataagacct gcatgaaaga
    35101 gcctgcaaac acacaacaca atacacaaca taaaaaagcc attttcactt gagggttatg
    35161 tatgaagaag tcgattctcg ctctgtcttt gttagtcggt ctctccgcag cggcttccag
    35221 ctatgcggcg ctaccggaga cggtacgtat cggaaccgat accacctatg cgccgttctc
    35281 gtcgaaagat gctaaaggtg attttgttgg ctttgatatc gatctcggta acgagatgtg
    35341 caaacggatg caggtgaaat gtacctgggt tgccagtgac tttgacgcgc tgatcccctc
    35401 actgaaagcg aaaaaaatcg acgccattat ttcgtcgctt tccattaccg ataaacgtca
    35461 gcaggagatt gccttctccg acaagctgta cgccgcagat tctcgtttga ttgctgccaa
    35521 aggttcaccg attcagccaa cgctggattc actgaaaggt aaacatgttg gtgtgctgca
    35581 gggatcaacc caggaagctt acgctaacga gacctggcgt agcaaaggcg tggatgtggt
    35641 ggcctatgcc aaccaggatt tggtctattc cgatctggct gcaggacgtc tggatgctgc
    35701 gttacaagat gaagttgctg ccagcgaagg attcctcaag caacctgctg gtaaagcttt
    35761 cgcctttgct ggctcatcag taaaagacaa aaaatacttc ggtgatggca ccggtgtagg
    35821 gctacgtaaa gatgatgctg aactgacggc tgccttcaat aaggcgcttg gcgagctgcg
    35881 tcaggacggc acctacgaca agatggcgaa aaagtatttc gactttaatg tctacggtga
    35941 ctgatacgtc gctgggaagc tgtacctgat ggaatgatca tcatggtgca cgccaggttt
    36001 gttgcactat cgtggtgcat tgaaatgcat acttaagcat ttttaatgaa aaataatacg
    36061 tctaacgggg cggaatattt tgccttgatg gtcaatttta tggcacgata agtgtaacaa
    36121 acctgtaaat attccctata aaaagactgt cagttgagga cattatgaaa aaactggtgc
    36181 tatcgctctc tctggttctg gccttctcca gcgcaactgc ggcatttgct gcgattccgc
    36241 aaaacatccg catcggtacc gacccgacct atgcgccatt tgaatcaaaa aattcacaag
    36301 gcgaactggt tggcttcgat atcgatctgg caaaggaatt atgcaaacgc atcaatacgc
    36361 aatgtacgtt tgtcgaaaat ccgctggatg cgttaatccc gtccttaaaa gcgaagaaga
    36421 ttgacgccat catgtcatcg ctttccatta cggaaaaacg tcagcaagaa atagccttca
    36481 ccgacaaact gtacgctgcc gattctcgtt tggtggtggc gaaaaattct gacattcagc
    36541 cgacagtcga gtcgctgaaa ggcaaacggg taggcgtatt gcagggcacc acccaggaga
    36601 cgttcggtaa tgaacattgg gcaccaaaag gcattgaaat cgtctcgtat caggggcagg
    36661 acaacattta ttctgacctg actgccggac gtattgatgc cgcgttccag gatgaggtcg
    36721 ctgccagcga aggtttcctc aaacaacctg tcggtaaaga ttacaaattc ggtggcccgt
    36781 ctgttaaaga tgaaaaactg tttggcgtag ggaccggcat gggcctgcgt aaagaagata
    36841 acgaactgcg cgaagcactg aacaaagcct ttgccgaaat gcgcgctgac ggtacttacg
    36901 agaaattagc gaaaaagtac ttcgattttg atgtttatgg tggctaatcg ccatcggtga
    36961 aagaaagccc gctcccttcg ggtaaccgga gggagacaag aggcggtaat tcaccacaca
    37021 cgacaggaca ggcagcatgt tgtatgggtt ttcaggtgtt attttacagg gtgcgctcgt
    37081 cacgctggag ctggctatca gctctgtagt gctcgctgta atcatcggtt taattggcgc
    37141 tggcggtaag ctctcgcaaa atcggctttc ggggctgatt ttcgaagggt acaccacgct
    37201 gattcgtggc gtgccggact tagtgttgat gctgctgatt ttctacggtt tgcagattgc
    37261 gctaaacacg gtgacggagg cgatgggcgt cgggcagatt gatatcgatc cgatggtcgc
    37321 tggtattatc actctcggtt ttatctacgg tgcttatttt accgaaacgt tccgtggtgc
    37381 ttttatggca gtgccgaaag gacatataga ggcggcgacg gcgttcggtt ttactcgtgg
    37441 gcaagtgttt cggcggatca tgtttccggc gatgatgcgt tacgccctgc caggcattgg
    37501 caacaactgg caggtgatcc tcaaatctac cgcactggtt tcgttactcg gcctggaaga
    37561 tgtggtcaaa gccacccaac tggcaggcaa aagtacctgg gaaccgttct atttcgccat
    37621 cgtctgtggc gtgatttacc tggttttcac cactgtttcc aatggtgtgc tgctgttcct
    37681 tgagcgccgc tactccgtgg gtgtgaagag ggctgacctg tgatcgaaat cttacatgaa
    37741 tactggaaac cgctgctgtg gaccgacggt tatcgcttta ctggtgtggc gatcactctg
    37801 tggctgctta ttttgtcggt agtgataggc ggagtcctgg cgctgtttct ggcgattggt
    37861 cgtgtctcca gtaataaata catccagttt ccaatctggt tatttaccta tatttttcgc
    37921 ggtacgccgc tgtatgttca gttgctggtg ttctattccg gcatgtacac gcttgagatt
    37981 gttaagggaa ccgaattcct taacgctttc ttccgcagtg gcctgaactg taccgtgctg
    38041 gcgctgacgc ttaacacctg cgcttacact accgagattt ttgctggggc aatccgttcg
    38101 gttccgcatg gggaaattga agccgccaga gcctatggct tctcgacttt taaaatgtat
    38161 cgttgcatta ttttgccttc tgcgctgcgt attgcgttac cggcatacag caacgaagtg
    38221 atcctgatgc tgcactctac tgcgctggcg tttacggcca ctgtgccgga tctgctgaaa
    38281 atagcccgcg atattaacgc cgccacgtat caacctttta ccgccttcgg cattgccgcg
    38341 gtgctctatt taatcatctc ttatgtcctg atcagcctct ttcgcagagc ggaaaaacgc
    38401 tggttgcagc atgtgaaacc ttcttcaacg cactgagaat acgatgtccg agaataaatt
    38461 aaacgttatc gatttgcaca aacgctacgg cgaacatgaa gtgctgaaag gggtctcact
    38521 gcaagcgaat gccggagatg taataagca
//
LOCUS       DAJTYZ010000026        37287 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000026 DAJTYZ010000000
VERSION     DAJTYZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.026"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..373
                     /locus_tag="OO596_001273"
     CDS             <1..373
                     /locus_tag="OO596_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6717176.1"
                     /translation="PAGYKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGA
                     MLAAVWLAVASTMKEPPYVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSA
                     YVKIDSKVTNRFEVEQAIRQA"
     gene            complement(501..992)
                     /gene="yajQ"
                     /locus_tag="OO596_001274"
     CDS             complement(501..992)
                     /gene="yajQ"
                     /locus_tag="OO596_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6717177.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1160..2071
                     /gene="panE"
                     /locus_tag="OO596_001275"
     CDS             1160..2071
                     /gene="panE"
                     /locus_tag="OO596_001275"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6717178.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2034..2624
                     /gene="yajL"
                     /locus_tag="OO596_001276"
     CDS             2034..2624
                     /gene="yajL"
                     /locus_tag="OO596_001276"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6717179.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(2678..4126)
                     /gene="thiI"
                     /locus_tag="OO596_001277"
     CDS             complement(2678..4126)
                     /gene="thiI"
                     /locus_tag="OO596_001277"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6717180.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4332..4574
                     /gene="xseB"
                     /locus_tag="OO596_001278"
     CDS             4332..4574
                     /gene="xseB"
                     /locus_tag="OO596_001278"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6717181.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            4574..5473
                     /gene="ispA"
                     /locus_tag="OO596_001279"
     CDS             4574..5473
                     /gene="ispA"
                     /locus_tag="OO596_001279"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6717182.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5498..7360
                     /gene="dxs"
                     /locus_tag="OO596_001280"
     CDS             5498..7360
                     /gene="dxs"
                     /locus_tag="OO596_001280"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6717183.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7415..8389
                     /gene="yajO"
                     /locus_tag="OO596_001281"
     CDS             7415..8389
                     /gene="yajO"
                     /locus_tag="OO596_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6717184.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(8443..8961)
                     /gene="pgpA"
                     /locus_tag="OO596_001282"
     CDS             complement(8443..8961)
                     /gene="pgpA"
                     /locus_tag="OO596_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6717185.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(8939..9916)
                     /gene="thiL"
                     /locus_tag="OO596_001283"
     CDS             complement(8939..9916)
                     /gene="thiL"
                     /locus_tag="OO596_001283"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6717186.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(9994..10413)
                     /gene="nusB"
                     /locus_tag="OO596_001284"
     CDS             complement(9994..10413)
                     /gene="nusB"
                     /locus_tag="OO596_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6717187.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10433..10903)
                     /gene="ribE"
                     /locus_tag="OO596_001285"
     CDS             complement(10433..10903)
                     /gene="ribE"
                     /locus_tag="OO596_001285"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6717188.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(10992..12095)
                     /gene="ribD"
                     /locus_tag="OO596_001286"
     CDS             complement(10992..12095)
                     /gene="ribD"
                     /locus_tag="OO596_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6717189.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12099..12548)
                     /gene="nrdR"
                     /locus_tag="OO596_001287"
     CDS             complement(12099..12548)
                     /gene="nrdR"
                     /locus_tag="OO596_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6717190.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            12699..13238
                     /locus_tag="OO596_001288"
     CDS             12699..13238
                     /locus_tag="OO596_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6717191.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            13537..14421
                     /gene="tsx"
                     /locus_tag="OO596_001289"
     CDS             13537..14421
                     /gene="tsx"
                     /locus_tag="OO596_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6717192.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14598..14945)
                     /locus_tag="OO596_001290"
     CDS             complement(14598..14945)
                     /locus_tag="OO596_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6717193.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15074..16045)
                     /gene="secF"
                     /locus_tag="OO596_001291"
     CDS             complement(15074..16045)
                     /gene="secF"
                     /locus_tag="OO596_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6717194.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16056..17903)
                     /gene="secD"
                     /locus_tag="OO596_001292"
     CDS             complement(16056..17903)
                     /gene="secD"
                     /locus_tag="OO596_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6717195.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(17931..18263)
                     /gene="yajC"
                     /locus_tag="OO596_001293"
     CDS             complement(17931..18263)
                     /gene="yajC"
                     /locus_tag="OO596_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6717196.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18286..19413)
                     /gene="tgt"
                     /locus_tag="OO596_001294"
     CDS             complement(18286..19413)
                     /gene="tgt"
                     /locus_tag="OO596_001294"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6717197.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19469..20539)
                     /gene="queA"
                     /locus_tag="OO596_001295"
     CDS             complement(19469..20539)
                     /gene="queA"
                     /locus_tag="OO596_001295"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6717198.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20632..21213
                     /gene="acpH"
                     /locus_tag="OO596_001296"
     CDS             20632..21213
                     /gene="acpH"
                     /locus_tag="OO596_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR6717199.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(21218..23035)
                     /gene="malZ"
                     /locus_tag="OO596_001297"
     CDS             complement(21218..23035)
                     /gene="malZ"
                     /locus_tag="OO596_001297"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6717200.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(23191..24564)
                     /gene="proY"
                     /locus_tag="OO596_001298"
     CDS             complement(23191..24564)
                     /gene="proY"
                     /locus_tag="OO596_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6717201.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24640..25959)
                     /gene="brnQ"
                     /locus_tag="OO596_001299"
     CDS             complement(24640..25959)
                     /gene="brnQ"
                     /locus_tag="OO596_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6717202.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26366..27661)
                     /gene="phoR"
                     /locus_tag="OO596_001300"
     CDS             complement(26366..27661)
                     /gene="phoR"
                     /locus_tag="OO596_001300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6717203.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(27719..28408)
                     /gene="phoB"
                     /locus_tag="OO596_001301"
     CDS             complement(27719..28408)
                     /gene="phoB"
                     /locus_tag="OO596_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6717204.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDCAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28598..29800
                     /gene="sbcD"
                     /locus_tag="OO596_001302"
     CDS             28598..29800
                     /gene="sbcD"
                     /locus_tag="OO596_001302"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6717205.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            29797..32940
                     /gene="sbcC"
                     /locus_tag="OO596_001303"
     CDS             29797..32940
                     /gene="sbcC"
                     /locus_tag="OO596_001303"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6717206.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33066..34250
                     /gene="araJ"
                     /locus_tag="OO596_001304"
     CDS             33066..34250
                     /gene="araJ"
                     /locus_tag="OO596_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6717207.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(34393..35301)
                     /gene="mak"
                     /locus_tag="OO596_001305"
     CDS             complement(34393..35301)
                     /gene="mak"
                     /locus_tag="OO596_001305"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6717208.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            35426..36337
                     /gene="rdgC"
                     /locus_tag="OO596_001306"
     CDS             35426..36337
                     /gene="rdgC"
                     /locus_tag="OO596_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6717209.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36415..36870)
                     /locus_tag="OO596_001307"
     CDS             complement(36415..36870)
                     /locus_tag="OO596_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717210.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(36867..37202)
                     /locus_tag="OO596_001308"
                     /pseudo
     CDS             complement(36867..37202)
                     /locus_tag="OO596_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9273 a        10058 c         9432 g         8524 t
ORIGIN      
        1 gccagcaggt tacaaaggta cggcgatggg tgtctactcc accagccagt ttcttggcgt
       61 ggcgattggc ggttcgctgg gcggctggat tgacggcatg tttgacggtc agggggtatt
      121 tctcgctggc gcaatgctgg ccgcagtgtg gctggcagtc gccagtacca tgaaagaacc
      181 gccgtatgtc agcagtttgc gcattgaaat cccggcggac attgccgcaa acgaagcgtt
      241 aaaagtgcgt ttgctggaaa ctgaaggcgt caaagaagtg ttgattgcag aagaagagca
      301 ttcagcttat gtgaaaatcg acagcaaagt gacaaatcgc tttgaggttg aacaggcaat
      361 tcgtcaggca taaaaaacgc ggcgaattgc ctgctgcgct acgcttacca ggcctacaaa
      421 gttcttgagt tacaaaccgt tgtaggccga atcaggcgtt catgccgcat ctgacatgaa
      481 caaaacgcac attgtcgcga ttaatcgcgg aagtttttga actggaacgg ctggccgaga
      541 tcgccaccac gtaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcaca
      601 cggatctcat cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt aatcatcttg
      661 acgattttct tctgggtcgc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag
      721 gttttaccgc tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc
      781 ttcagcagct tggcacgcag aatatccagc aactgattga cctggaaatc ggactcgctc
      841 aacactttga tggttttgct ggcgtcgttc agctcaaatg aggcttcaac attacggaag
      901 tcaaaacggg actccacttc gcggctcgcg ttatcgaccg cgttacgtgc ttcctgaaga
      961 tcaacttcag agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg
     1021 aagcataata cccgcaaagt taaataaccg acagtcctgc accaggacgt atcccttaat
     1081 tgtgcatagt cacaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg
     1141 ttgcgggtga ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt
     1201 atggcttaca gcactttgta aacagggtca cgaagttcag ggctggctgc gcgtaccgca
     1261 accttattgt agtgtgaatc tggttgagac tgatggttcg atatttaacg aatcgctgac
     1321 cgccaacgat cccgattttc ttgccaccag cgatctgctc ctggtgacgc tgaaagcatg
     1381 gcaggtttcc gatgccgtca aaagcctcgc gtccacactg cctgtaacca cgccaatact
     1441 gttaattcac aacggcatgg gcaccatcga agagttgcaa aacattcagc agccattact
     1501 gatgggcacc accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa
     1561 cggaatcacg catattggtc cggcacggca acaggacggc gattacagct atctggcgga
     1621 tattttgcaa accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg
     1681 gcgcaagctg gccgtcaact gtgtgattaa tccactgacc gccatctgga attgcccgaa
     1741 tggtgaatta cgtcatcatc cgcaagaaat tatgcagata tgcgaagaag tcgcggcagt
     1801 gatcgagcgc gaagggcatc atacttcagc agaagatttg cgtgattacg tgatgcaggt
     1861 gattgatgcc acagcggaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca
     1921 caccgaaatc gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc
     1981 cgtaccggaa aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg
     2041 catcggcact ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accactatcg
     2101 atctgctggt tcgcggcggt atcaaagtca ccactgccag cgtcgccagc gatggcaacc
     2161 tggccattac ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaggtgg
     2221 ctgatggcga atatgacgtg atcgtactgc ctggtggcat taaaggcgca gagtgttttc
     2281 gcgatagtac cctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg
     2341 cggctatttg cgccgcgcca gccaccgtgc tggtaccgca cgatatcttc ccgattggca
     2401 atatgaccgg cttcccgacg ctgaaagaca aaattcccgc cgaacaatgg caggacaagc
     2461 gcgtcgtctg ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acggctattg
     2521 attttggtct gaaaattatc gacctgctgg ttgggcgcga gaaaacccat gaagtggcat
     2581 cacaactggt gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa
     2641 cgtcgcatct gaccagatgc gacgtaaaaa ccacaaatta tgggcgatac accttcacat
     2701 tgttaaagcc ctgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac
     2761 accacagtag ccaggttctg ttctggtcga gatcgccaaa tttggtgctc agtttataga
     2821 acggcagaga aaccacgtcg ataccttcaa ccttcagcgg cttatcttcc tgctcatcga
     2881 cagaacggat atcgaggatc acgtcgttcg ggccaaagcc attgacggtt tccacttcca
     2941 ccacttcctg ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa
     3001 ccactttatc gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa
     3061 ccgctttcac cgtcgggctt ttggagatca cgccacaata ttctggcatc gtgcgagcaa
     3121 agtcttcagt gccaatctgg cgggccaggt tgatgatgtg ctctttgtcg taagaaatca
     3181 gcggacgcag gatcagcgta tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct
     3241 gactggacac ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag
     3301 ccactttaga cgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt
     3361 cgattttctc gagaatttcc ccgacgaccg gttcgaaatt aatggcgaca aaacgcacgc
     3421 ggtgggagct gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat
     3481 gcgctgcgcc gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca
     3541 tataactgga aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg
     3601 tgccaatcgg gaaaccgcca ataccttcgt agcggccttt aatcagcaga agacgatcgt
     3661 cttccacttc cagatggaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa
     3721 tatgctgatt taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt
     3781 tgccacggcg cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatattgaa
     3841 ccaacgcttt ctcgaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa
     3901 tatggtggat acccggaata cgggtcaggg cgtcgcgaat agccagacgc tgtttttcat
     3961 cttttgcgcg aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt
     4021 gctttaaaac gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt
     4081 ggcttttgat ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc
     4141 gttggcaagc cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc
     4201 atccaggcgc ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc
     4261 gcgttatttg ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc
     4321 agacattcat tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg
     4381 agctggaaca gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga
     4441 acgagttcga acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg
     4501 aacagcgcgt acaaattctg ctgtctgaca atgaagacac ctctctaccc ccttttacac
     4561 cggacaatga gtaatggact ttccgcagca acttgaagcc tgcgttaagc aggccaacca
     4621 ggcgctgagc cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat
     4681 gcagtatggc gcattattag gtggtaagcg cctgcgacct tttctggttt atgccaccgg
     4741 tcatatgttc ggcgttagca caaacacgct ggacgcaccc gctgccgccg ttgagtgtat
     4801 ccacgcttac tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg
     4861 cggtttgccg acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc
     4921 tttacaaacg ctggcgttct cgattttaag cgatgccgat atgccggaag tgtcggaccg
     4981 cgacagaatt tcgatgattt cagaactggc aagcgccagc ggtattgccg gaatgtgcgg
     5041 tggtcaggca ttagatttag atgcggaagg caaacacgta cctctggacg cactcgagcg
     5101 tattcatcgt cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag
     5161 cgccggagat aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg
     5221 ccttgccttc caggttcagg atgacatcct ggatgtggtg ggagatactg caacgttggg
     5281 aaaacgccag ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct
     5341 tgagcaagcc cggaagaaag cccgggatct gatcgacgat gcccgccagt cgctgaaaca
     5401 actggctgaa caatcactcg atacctcggc actggaagcg ctagcggact acatcatcca
     5461 gcgtaataaa taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata
     5521 cccgaccctg gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt
     5581 accgaaactc tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg
     5641 gcacttcgcc tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa
     5701 caccccgttt gaccaattga tttgggatgt ggggcatcag gcttatccgc ataaaatttt
     5761 gaccggacgc cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc atccgttccc
     5821 gtggcgcggc gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag
     5881 tgccggaatt ggtattgcgg ttactgccga gaaagaaggc aaaaatcgcc gcaccgtctg
     5941 tgtcattggc gatggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg
     6001 cgatatccgt cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa
     6061 tgtcggcgcg ctcaataacc atctggcaca gctgctttcc ggtaagcttt actcttcgct
     6121 gcgcgaaggc gggaaaaaag ttttctctgg cgttccgcca attaaagagc tgctcaaacg
     6181 taccgaagaa catattaaag gcatggtagt gcctggcacg ttgtttgaag agctgggctt
     6241 taactacatc ggcccggttg acggtcacga tgtgctgggg cttatcacca cgctgaagaa
     6301 catgcgcgac ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta
     6361 tgaaccggca gaaaaagacc ccatcacttt ccacgccgtg cctaaatttg atccctccag
     6421 cggttgtttg ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg
     6481 gttgtgcgaa acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga
     6541 aggttccggc atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggcaat
     6601 cgccgagcaa cacgcggtga cctttgccgc cggtctggcg attggtgggt acaaacccat
     6661 tgtcgcgatt tactccactt tcctgcaacg cgcctatgat caggtgctgc atgacgtggc
     6721 gattcaaaag ctcccggtcc tgttcgccat cgaccgcgcg ggcattgttg gtgctgacgg
     6781 tcaaacccat cagggcgctt ttgacctctc ttacctgcgc tgcataccgg aaatggtcat
     6841 tatgaccccg agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa
     6901 cgacggcccg tccgcggtgc gctacccgcg cggtaacgcg gttggcgtgg aactgacgcc
     6961 gctggaaaaa ctgccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggcgat
     7021 ccttaacttt ggtacgctga tgccagacgc ggcgaaagtc gctgaatcgc tgaacgctac
     7081 gctggtcgat atgcgttttg tgaaaccgct tgatgaagcg ttaattctgg aaatggccgc
     7141 cagccatgaa gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggcagcgg
     7201 cgtgaacgaa gtgctaatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc
     7261 tgacttcttt attccacaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc
     7321 cgccggtatg gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta
     7381 tgcttaagaa attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac
     7441 cgaccttcgc gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgtgg
     7501 taatcacgca tggacactac cggaagagag tagccgtccc atcattaaac gcgcgctgga
     7561 aggcggcata aatttctttg ataccgccaa tagctattcc gatggcagca gcgaagagat
     7621 cgttggtcgc gcactgcggg atttcgcccg tcgtgaagac gtggtcgttg cgacgaaagt
     7681 gttccatcgc gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat
     7741 caacgacagc ctgcgccgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg
     7801 ggattacaac acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg
     7861 gaaagcgcgt tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggccctgaa
     7921 gctacaaaaa cagcacggct gggcgcagtt tatcagtatg caggatcact acaatctgat
     7981 ttatcgcgaa gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat
     8041 tccgtggagc ccgctggcgc gggggcgact gacgcgtccg tggggagaaa ctaccgcacg
     8101 actggtgtct gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca
     8161 gatcgcagag cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc
     8221 gctggcctgg ttattgagta aaccgggcat tgccgcaccg attatcggta catcgcggga
     8281 agaacagctt gatgagctgc tgaacgcggt ggatatcact ttgaagccag aacagattgc
     8341 cgaactggaa acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc
     8401 tgatgagacg tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag
     8461 cggccagtga tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc
     8521 gtcgatcatg atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg
     8581 cttccacata tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt
     8641 ggtcggcagc gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc
     8701 gtgatcgtgc acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat
     8761 ccccaacatc accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa
     8821 cggaatcgct gccagcgatc ccatcgtgcc aggaacgatt gggcttaatc cactcccgaa
     8881 tccgacagca agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt
     8941 atggcgtggc aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa
     9001 taaaacaaag cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa
     9061 ggtgtccgag agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact
     9121 cgtaatcttc accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag
     9181 aaagcgcatc agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt
     9241 tcacgatatg cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca
     9301 gatcgcgcag tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca
     9361 agtagtcagc atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg
     9421 cggcgctatc gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc
     9481 gcgttaaggc tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc
     9541 cacgcgtggt atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac
     9601 tgtcgctgaa ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca
     9661 gccaggccgg atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat
     9721 aagccagatc agcaggatcg atatcaggga ggaaatggtt acccgccacc agcgtatcag
     9781 tgctgatcgc cagggtctgt ttctcgggga tattgagaag tgcgcaatcg tcgccgatgc
     9841 ccagttcgac atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga
     9901 actcgccaca tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc gacgatcatg
     9961 gaacggtctt ccgtgaatct accggcctgg atatcacttt ttgttagggc gaatcacagg
    10021 tgctgcttta tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt
    10081 cgccagttcg atcgcttcgt taatggccac tttgtatggc acatcgctac gtttagacag
    10141 ttcgtacagc gcaatgcgca gtactgcttt ttctacctgt cccagttctt ccagcaggcg
    10201 ggacaggtat ggcttcatca gtccgtcgag gtatgcggta ttagtcgcca ccccggccag
    10261 cagctcacgg aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta
    10321 ttcaacatca gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca
    10381 ctcacgagcg cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct
    10441 tgatggcttt caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt
    10501 tgccagcttt ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc
    10561 caaaagcaac cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac
    10621 cagcgacata ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg
    10681 cgtcgtattt accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag
    10741 gcacccaaac aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa
    10801 ttgcaccttc cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga
    10861 cgcgagcgtc cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc
    10921 gagtatggcc ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc
    10981 gagccgggag atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc
    11041 tctttgaatt taaattgggg ggcgtcggct aatttctcaa gccctggcag cgtgcataat
    11101 ccgcgggcgt cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa
    11161 cccgcctgca gcaatgcgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatt
    11221 tgctgtttac ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga
    11281 atcagcaagg tacgcaccgt ttccggccac tcactagaat cttcctgcgt acgcgcgaac
    11341 caggtttcgc cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca
    11401 atcacaatac gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca
    11461 tccagttcag accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg
    11521 gttaaaatgg catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa
    11581 gtgatccact ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt
    11641 tttaactgaa tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc
    11701 tcggcttcac tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa
    11761 agtccacgcc cagcgacctg cgggttagga tcctgcatcg ctgcgaccac gcgcgctacg
    11821 ccagcggcga tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg
    11881 agtgtcacat acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact
    11941 tcggcatgtg gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg
    12001 acaatgacgc ccccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc
    12061 tttagcgccc gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc
    12121 tcttcgccaa attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag
    12181 gcgactttat cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt
    12241 acttcgcgct caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg
    12301 tcatcggaac tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct
    12361 tcattaaacg gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc
    12421 acttcaaagg tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat
    12481 gaaccctcgc ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg
    12541 caatgcatac ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg
    12601 atacaacaaa ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct
    12661 ctaccattag gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac
    12721 tgctcctgct gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac
    12781 agatgaaaca cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag
    12841 tcaaaatcac tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc
    12901 ctggagcgaa aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg
    12961 tgaatattac gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga
    13021 atgatccctt gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag
    13081 acaactttca ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg
    13141 cccccagcga tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct
    13201 ttgtacgtat ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt
    13261 gcgtcccgca acatctttcc ccgtcatttt gttactctgc ttacatcacc tggattgata
    13321 gtaaaagttt gcaacaaggg cgaaagtcag tacaatcccc gcccgaatgt gtgtaaacgt
    13381 gaacgcaatc gattacgtaa atgatagaac tgtgaaacga aacatatttt tgtgagcaat
    13441 gatttttata ataggctcct ctgtatacga aatatttaga aacgcaattt gcgccttttt
    13501 cactcccgca agggattttc aaacagtggc atacatatga aaaaaacatt actggcagcc
    13561 ggtgcggtac tggcgctctc ttcgtctttt actgtcaacg cagctgaaaa cgacaaaccg
    13621 cagtatcttt ccgactggtg gcaccagagc gttaacgttg tcggaagcta tcacacccgt
    13681 ttcggaccgc agatccgcaa cgatacctac cttgagtacg aagcattcgc taaaaaagac
    13741 tggttcgact tctatggtta tgcggatgcg ccggtattct tcggcggtaa ctccgatgct
    13801 aaaggtatct ggaaccacgg ttctccgctg tttatggaaa tcgaaccacg tttctccatc
    13861 gacaagctga ccaatactga ccttagcttc ggtccgttca aagagtggta cttcgcgaac
    13921 aactacattt acgacatggg tcgtaataaa gatggtcgcc agagcacctg gtacatgggt
    13981 ctgggtaccg acatcgatac tggtctgccg atgagcctgt ccatgaacgt ctatgcgaaa
    14041 taccagtggc agaactatga cgcagcgaac gaaaacgagt gggacggtta ccgtttcaaa
    14101 attaaatact ttgtgccgat taccgatctg tggggcggtc agctgagcta catcggcttc
    14161 accaacttcg actggggttc cgatttaggg gatgacagcg gtaacgcaat caacggtatt
    14221 aagacccgta ctaataactc tatcgcttcc agccatattc tggctctgaa ctacgatcac
    14281 tggcactact ctgtcgtagc tcgttactgg cacgacggtg gtcagtggaa cgacgatgca
    14341 gaactgaact tcggcaacgg caacttcaac gttcgctcta ccggctgggg tggttacctg
    14401 gtagtaggtt acaacttctg attatgaaaa tgccgggatt tattcccggc atttctgatt
    14461 gttaacaacg tgtgctttgt tcatgccgga tgcggcgtaa acgctttgtc cggcccacaa
    14521 aagcacgtaa attcaatata ttgctgaaat aacgtaggcc tgataagcgt agcgcatcag
    14581 gcaattttac gttttaatca cttcttcttg ttcatcatcg ctttcaggtc agcgaatggg
    14641 ttgtacttcg cttcaccgac atctttctgc gcatcttccc ctgcgataac ggtcgtacca
    14701 tactgatccg cttcggtata tttcgaatgc tcatgatcgt ggcaatagag acaaagcaat
    14761 tcccagttac tgccatcttc cgggttattg gtatggtcgt gatcaatgtg gtgaacggta
    14821 agttcacgca ggttggaata aacaaactcg cgggaacagc gaccgcagac ccacggatag
    14881 atttttaatg ctttttcgcg atagccgctt tctaaccgcg catagttttt tgggatgata
    14941 gccatgaaaa gttacctgga tgaaatgtag ggttacgcgc agtttcccaa gatgcgggga
    15001 ttttcgcaat ctccatacat caaaaaaaat cccgatcttc tgaccgggat tttcaacatc
    15061 aacgggaact tgattacggc agaattgacg gctgatccgc cccttctttt tccactttct
    15121 gctgcaacat gtgttcgcgc ttcataccca gtttcagagc caacgcagat gctacgtaga
    15181 tggaagatgc agtgccgatg gaaacaccga taagcatggt cagcgagaag ccttccagta
    15241 ccggaccacc gaagagatac agcatcagga taaccatcaa ggtagtaccg gatgtgatca
    15301 aggtacggtg cagcgtctgg gtcaaggaca cgttaaagat ttcgtaaggc gtaccgcgac
    15361 ggatcttgcg gaagttttca cgaattcggt ccgataccac gatactgtcg ttaagcgagt
    15421 aaccgataac cgacatcaac gatgccacaa tggtcaggtc aatctcgata tggaataacg
    15481 acaaaatacc cagcgtaatg ataacgtcgt gcgccagcgc aataaccacc cctgccgcca
    15541 gtcgccactc aaagcggaaa cctacgtaca cgaggataga cagcagcgct gccatcaacg
    15601 ccatcgcacc ggtttgcgca aggtctgccc ccacgctcgg accgacgaac tcaatacgct
    15661 tcactgctgc attctgattg gtggattcgt taatcacctt cagaacctgg ctgcccagca
    15721 cctgaccgcc ggtttcgcct tcagcaggcg gcatacggac catgatgtcg tggctgctac
    15781 caaagttttg cagcattggc tcttcaaaac cggctttttg caatgcatca cgcattacgt
    15841 caatttcagc cggtttttcg agcgtaattt cgataaccgt accaccggtg aaatccagcc
    15901 cccagttaaa gccgcgcacg cccataataa cgatagcagc gattaacagc agaccagaga
    15961 tgccgaaagc ccagtagtcc cagcgcataa agtcatagac tttacggccg tggtttagtt
    16021 gttcaacagt atattcctgt gccacatcgc actcctcaga ttgacagctt cttgacgcgc
    16081 ttgccgccat atagcaggtt tacgatggca cgcgtaccga caatcgcggt aaacatcgac
    16141 gtcgccacac cgataccggt agtaatcgcg aaccctttaa ttgccccggt acccactgcg
    16201 tacaggatga tgactttaat cagcgtggtg atgttcgcat cgaagataga actgaatgcg
    16261 ccacgataac cttcatcaat tgcctgttga acagtacgtc cgttgctcaa ctcttcttta
    16321 atacgttcgt tgatcagtac gttcgcatcg accgctaccg caagggttaa gacgataccc
    16381 gcgatacctg gcatactcag tgttgcgcct ggcaacagcg acataatacc gacgatcaag
    16441 atcaagttgg caatcagagc actggtcgca atcagaccaa acttcttata gaagatgatc
    16501 atgaacagaa tagaaaccag cagaccggca aggcaagcct ccagcccctg ctcaatgttc
    16561 tgcataccca gggtcgggcc aattgtacgt tcttcaacaa tctgaatcgg cgcgatcaac
    16621 gcaccggcac gcagcagcag tgacagctga cgggcttcgt tcgggttgtt gatgccggtg
    16681 atacggaagc tgttacccag acgagactgg atgttggcga tgttaatcac ctcttcctgt
    16741 ttcaccagaa ccgcacgacc atttgcatct ttcttaccgc tgtctttgta ctccacaaac
    16801 agggttgcca tcggtttgcc gatgttgtcc ttggtgaagt tagacataat gttgccacca
    16861 gcgctatcga gcgagatgtt aacctgcggc tggttgtatt cgtcctggct ggaagtggag
    16921 tcggtgatat ggtcaccggt cagaattacg cgtttgtaca gcacaaccgg ctgaccttcg
    16981 cgggtctgtt tcacttcaga gtcgcccggt acgcgaccgg atgccgcagc ggcctgatca
    17041 acattggtgt ttaccagacg gaattccagc gttgccgtcg cgcccagaat ctctttcgca
    17101 cgcgcagtgt cctgaatacc aggcagttca acaacgatac ggtcagcacc ctgacgctga
    17161 accaccggct ccgccacgcc aagttggttt acacggttac gcaggatatt aatgttctgc
    17221 tgcaccgcat attcacgcgc ttcactcaga cgagcatcgc tcattaccgc acgcagctgg
    17281 ttgctgccct ggctgctaat caccaggtcc ggatggcgct tgctcagata cgcaatggct
    17341 tcatcacgag ctttagcatc gcggaaagtg atgctcaggc cgtagttgtt ttctttacga
    17401 acagtggtat acgggatgcc cttttcgcgc aggtcactgc gtaggctatc gatattttgt
    17461 tcctggagtt tgccaagcgc ggtgtccata tccacttcca tcaggaagtg aacgccgcca
    17521 cgcaggtcaa ggccgagctt catcggctca gcatgaatag ctgccagcca gcgcggcgtt
    17581 gccggggcaa ggttaagcgc cacgacgtat ttgtcaccca taacgcccat taatgcttca
    17641 cgagcgcgca actgggtgtc agtggagtcg aagcgcgcaa gaatagcgcc ctcttccagt
    17701 gccacagact tagcagttat tttttcttct tgtaacgttt tctggacctg gatcagcgtt
    17761 tgctcactgg cggcgactcc gcgcgcacca gtgatctgaa cagccggatc ctcaccaaac
    17821 aggttgggaa gcgcatacag cagaccgatg acaatcacca cgatcagcat gacgtacttc
    17881 cacaaaggat aacggtttaa cacggcaatt cccttaggga aaaattttaa ttacagcgcc
    17941 ttcatggtgc ctttcggcag gacggcagct acgaagtcac gtttaataac tacttcagtg
    18001 gtgtcattca gcgcgatagc aatgtagccg ttttccgcta ctttggttac gcgaccaacc
    18061 aggccaccgt tcgtcagaac ttcatcacct ttggcaatgg agtccatcag ctttttgtgt
    18121 tctttggtgc gcttctgctg tggacgcagg atcatgaaat agaaaatcag accgaatacc
    18181 accagcatca aaatcaaaga catcgggcta ccttgcgccg gtgcacccgt tgccgctacc
    18241 gcatcagaaa taaaaaagct cattaaattt ccctcattat taatattaat caacgttcaa
    18301 aggtggtact tctcgcccct gacgctggta aaaatcagtt acgaagctct ctaatttacc
    18361 ctcttcaata gccttgcgta aacccgccat caaacgctgg tagtaacgaa ggttatgaat
    18421 ggtgttgagt cgcgcgccta atatttcgtt gcaacggtca agatgatgca agtaagcgcg
    18481 tgaataattg cgacaggtgt agcaatcaca ctcaggatca agtgggccag tatcgctctt
    18541 atacttcgca ttgcggattt tcaccacgcc atcggtcacg aacaaatgac catttcgggc
    18601 gttgcgggtt ggcattacgc agtcaaacat atcgatacca cgacgtacgc cttcaaccag
    18661 gtcttctggt ttaccaacgc ccatcaggta acgcggtttg tctgccggaa tttgcgggca
    18721 tacatgctcc agaatgcggt gcatatctgc tttcggctca cccacagcca gaccgccgac
    18781 agcgtagcca tcaaaaccga tatctaccag acctttaaca gaaatatcac gtaaatcttc
    18841 gtaaacgctg ccctgaatga taccaaacag cgcatttttg tttccgagac tgtcaaaacg
    18901 ctcacggcta cgcttcgccc aacgcagaga catctccatg gagcgttttg cgtaatccca
    18961 gtcagcagga tacggcgtac actcatcaaa gatcatgacg atatccgaac caagatcgta
    19021 ctgaatctcc attgattttt caggatcgag gaaaatcgga tcgccgttga tcgggttacg
    19081 gaagtgcacg ccctgttcgg tgattttacg aatatcgcca aggctgaaga cctggaagcc
    19141 gccggagtcg gtgaggatcg gccccttcca ctgcataaaa tcgtgcagat cgccgtgcag
    19201 tttcatgatt tcctggcccg ggcgcagcca caggtggaag gtgttgccga ggataatttg
    19261 cgcgccagtg gcttcaactt cttccggcgt catccctttt acggtgccgt aggtgccaac
    19321 aggcataaaa caaggcgttt ccactacgcc acgatcaaag accaggcggc cacggcgtgc
    19381 gcgaccgtcg gtggtgtcca gttcaaattt cattttttct ccactacgtc agaaaaacag
    19441 tccaacgttt taaaccagcg ccacggaatt actccccgac gcgctcatta attgcctgcg
    19501 gattgtacgt gataaacatc gcatcaccgt aactaaaaaa gcgatatttc tcttctaccg
    19561 ctgctttata ggcgttcatg gtgtgttgat aaccggcaaa ggccgaaacc agcataatca
    19621 gcgtcgattc cggcaggtgg aagtttgtca ccagcgcatc gaccactttg tactggaagc
    19681 ccggatagat aaagatttgg gtatcgtcaa agaacggttc gataagatcg tttttcgccg
    19741 cctgagccgc gctttccagc gaacgtactg aggtggtgcc aaccgcaatc acccggttac
    19801 cgcgcgcttt cgccgccagt accgcgtcta ccacatcctg cggcacttca gcgtattccg
    19861 agtgcatgat gtgatcttca atggtgtcga cgcgcaccgg ctggaaggtg cccgcaccaa
    19921 cgtgcaacgt cacaaacgcc atctccacgc ctttggcgcg taatttttcc agcaaaggtt
    19981 cgtcaaaatg cagacctgcg gtcggggctg caaccgcgcc cggtttttcg ctataaacgg
    20041 tttgataaag ttcgcggtca gcgtcttcgt ccggacggtc gatatacggc ggcagcggca
    20101 tatggccgat gctgttgaga atatccagca ccgagcgttc atcattaaat tcgacttcaa
    20161 acagtgcgcc gtggcgcgcg gtcattgttg cgttaatact ttcgtcatcg cccagcagca
    20221 gttctgcgcc aggttttggc gctttcgagg cgcgaatatg cgcaagaatg cgtttgtcgt
    20281 cgagcatccg ttcaaccagc acttcaatct tgccgccgct ggctttacgc ccaaacaggc
    20341 gcgccgggat cacgcgggta ttattaaaaa ccagaagatc gccggggttg agcttatcaa
    20401 gtaaatcggt gaaagtaccg tgcgtcagcg cgcccgtcgg cccgtccagc gacagtaaac
    20461 gacagctact gcgttcaggc atgggatagt gggcaatcag ggattcgggc aattcaaagg
    20521 agaaatcggt aacgcgcatg acactgactc agactaaaat aagaggcggg tagtctagtg
    20581 ccggggcgct ctccctgcaa cctttacccc cttttggata gaatataatc gatgaatttt
    20641 ttagctcacc tgcatttagc ccatctcgcg gaaagctcgc tttccggcaa tttactggct
    20701 gatttcgtac gcggaaatcc tgaagaaagt tttccgcccg acgtcgtggc tggcattcat
    20761 atgcatcgac gtatcgacgt attaaccgac aatctgccgg aagtccgcga agcacgggaa
    20821 tggtttcgta gtgaaactcg ccgcgttgcg cctattacgc tggatgtcat gtgggatcac
    20881 tttctttccc gccactggtc gcagctgtcg ccggactttc cgctacagga atttgtctgt
    20941 tatgcccgcg agcaagtgat gacgattttg ccggactcac cgccacgttt tatcaatctg
    21001 aacaattact tgtggtcaga gcagtggctg gtgcgctatc gcgatatgga tttcatccag
    21061 aacgtgttaa acggcatggc aagccgccgc ccacgtctgg atgccctgcg tgactcctgg
    21121 tacgatttag acgctcatta tgacgccctc gaaacccgct tctggcagtt ttatccgcgg
    21181 atgatggcgc aggcgtcaca caaggcgtta taaaacgtta gttcatccat accgtagccg
    21241 aaatggcagg caaagccaga atcccgtcag tcagttgccc atgcctttct ttgcattgcc
    21301 attgcacggc attgagcaac ggtgacgcgg gtagcacgac ttcacaagct tcgccacggt
    21361 tgattgccac cagtacacgc tgctgattca gcacgcggac aaataccacc acgttatctt
    21421 ccgcatacag cacctgacag ccgccatgac gtagcgcctg acttttctta cgcagcgcaa
    21481 tcattcgctg gtacagcgcg aataacgccg tatcctgctt ttccacctgc caggggaacg
    21541 gtttacggca aaacggatcg tttttgccat ccagtcctac ttcatcaccg taataaatgc
    21601 acggtacacc aggccaggtg aacagccaga ccaccgccag cggcaggcgc gcaatatccc
    21661 gaccgagcag cgttttaaat cgcgcagtat cgtggctgtc gagctgatta aacatacgta
    21721 attgttgttg atgagaaagc cctgcgcggt aattatccat ccaggccata caggtttggg
    21781 catcaatttg ctgcggatcg taagagatat cggtattggc aagaaatccc cacaacggga
    21841 atgtgaagcc acgatagttc atggcggcat cttccacatc ggcctgtaac cattgccgtg
    21901 catcgccaaa atgttcgccg acaatatacg cttccggctg ggtttctttc gccgcttcgg
    21961 tgctcccagc aacgtgctgc atattattgc gcgccccacc cgcctcccct agcatatgca
    22021 ccacatccag ccgccagccg tccatactcc acggcgcttt cagccaatgg cggacaatac
    22081 tgtcttcccc gcgataaatt tcattcacca gactttccga ctgataatcc agcttcggca
    22141 agctggcata gccaagccag tcgagcgccg tgccatcatc actaaacgag taccagtcgc
    22201 gccagggcga ttcagggttg tgacaagctc cccccgtgcc acgattgtgc ctatcaaacc
    22261 aggcatggga atcgccactg tggttaaaca cgccatccag ccccagccgc attcccagct
    22321 gctgcgtatt gtggcgtaag cgcagcaacg ccccatcacc gccaaactgc ggatcgacat
    22381 ggcgataatc ctcggtatcg tatttatgta cgctgggagc tttaaacacc ggattgagat
    22441 acagcgccgt cacgccaagc tttttcagat acggcagttt ttcgcttatc ccgtccagat
    22501 cgccgccata gaacgttgat ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca
    22561 agatgatctc ttgtccggct gcatgatggt aatagacatg atcctgttca gcttcacgag
    22621 gaagactacg cgcaaaacga tcagggaaga tctgataaaa aatctgatcc gcagcccatt
    22681 gtgggccgat atccggtaca tcgacggcaa actgctccag tcgtgccggc ggcattcggc
    22741 tgaagccctg cggtgtaaac caacgctggc gatcgtgcca cagcagtttg aaactgtaac
    22801 gccgccgggg ttgtccgctg gagagatcaa tcgccgcacg ccatgcggtg acgccaggct
    22861 gctgctgact gcgatgctta tgcatcgata ctgacatttc ttcgttatcg tgttctgtac
    22921 gcagcataat gcgctgcggt gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat
    22981 ctttgctttg tttaacaaat gggggcaccg gcaggtgcca tgcatttaac atcataattc
    23041 ccctgtgatg aaaatctgat caccttgcca tagcctaatg caggatcact catcatctga
    23101 aggatatttg ggggaggagg acgggggcgg ataactttcg gaatagccgg atcaggattt
    23161 cctcctgaac cggcttacag aacgcaagga ttactggttt tcagccagct gacgatcgtg
    23221 gcgacgctta aacatccagc caatcaacag cacaacaatc cacgcgaaac cgacatacag
    23281 cgagatacgc gtatccgggt gataaccaat caacccgata ataaagagca ggaaaatcag
    23341 cccgccgatg gtcgttgcta ccccacccgg cactttaaat ttcagcgcct taacttcttc
    23401 tggcggcaaa cagcgacgga aggcaatttg cgacagcagg atcataatcc acacccacac
    23461 cgtggcgaag gttgccagcg aagcgatcac caggaagacg ttttccggca tgatgtagtt
    23521 cagatacacc gcaaacagca gcgcggtagt catcaccagc accgtaaccc acggaatacc
    23581 gcgacgcgac gttttgctga aaattttcgg cgcgctgccc tgctctgcca taccgtggag
    23641 catacggcct acgccaaaga cgtcactgtt aattgccgat agcgaagcgg tcagcacaac
    23701 aaagttaaga atgctagcgg caaaggtaat gcccatatgc tggaacgtca gcacgaacgg
    23761 gctaccggca gtgccaacct gattccacgg gtagatagac ataatgacga acagcgtacc
    23821 gacgtagaac accagaatac gcatcggcac ggagttaatc gcacgcggta tcgatttctc
    23881 aggatctttc gcttcaccgg cggtaatccc gataatttcg atcccaccgt aagcaaacat
    23941 caccatttgc aacgacatca ccatcccaag ccagccgtta ctgaagaagc cgccgttgct
    24001 ccacagatta tgaataccgg tcggttgccc gccgttgcca atcccccaga tgatgatgcc
    24061 gaaaccagcg acaatcatga tgatgatggt ggcgacttta aagaacgaga accagaattc
    24121 cagctccccg aagaccttca cgctcatcag gtttacggcg caaatgatca gcaccacgct
    24181 cagtacccaa atccagtgcg gcaccgtcgg gaaccagaca cccatataga taccaaaagc
    24241 ggtcacatcg gcgatggcga caataaggat ttcaaagcag taggtccagc cggtaatgta
    24301 acctgccagc gggccgaggt tttcctgcgc ataacgcgag aaagagctgg cggccgggtt
    24361 atgtaccgac atttccccca gcgcacgcat aatgatatac gccgcgatac caccgataat
    24421 ataggccaac aacacgctcg gaccggccat tttgatggcg tctgccgaac cgtaaaacag
    24481 cccggtacca attgctgaac ccagtgccat aaagcgtatg tggcgggtac ttagcccacg
    24541 ctttagcttg ttcttacttt ccatcaattc ctaacccatc aacacaataa aaaaccacgg
    24601 ggcatcgcag ccccgtggtt aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac
    24661 ctgacgacct gccgcgcgat cccagataat ggccagaacc accatcacca ctgttggcat
    24721 taaccacgcc agaccttgtt cggccagcgg taaacgctgc gcccaggacg gtaagatatc
    24781 gctgaatgca gatgctttga tcccgtcgag aataccaaaa agcaggctga taaacatcgg
    24841 cggagcaatc acgcgggacg aattatgcca ccatgagcgt gtaaaactta atacaaccag
    24901 tgcgatacac ggcggataaa tagcggtcag caccggtacg gagatctgga tcagctggct
    24961 taagccgagg ttagaaacca ccatcgagaa gccgccgagg ataaacacca gcgtacgata
    25021 agagagcggt acgtactggg caaagaattc tgcacaagca caggtcaggc caactgccgt
    25081 taccaagcag gcgatgaaga ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt
    25141 gtgctgaacg taagcatgca gaatagcagc gccgtttgca gactgatcga ccagcgacgc
    25201 gctgtctgaa cccagacgga acagcgccag gtacagcaga gtcagaccaa cacccgccat
    25261 caggccagcc cagacggtat aacgggtcag cagacgcgct tcggtaacgc cacgagaacg
    25321 cgccgcgtta acaataacga taccaaacac cattgcgccc agcgtatcca tggtcagata
    25381 gccgttaacg aagccgttag aaaacgcagc gttttgataa gcctcagtcg ccgtgctgat
    25441 agaacccgcc ggccagacaa tcgcggcaac agacaggatg accagcgcga taattttcag
    25501 cggcgcaagg aagttgccca cggtatccag cagcttgccc ggatagagcg aaaccagaat
    25561 aacgatagcg aaatagacca ggctgtagat aaacagcggc agcgcggaat caccagtcag
    25621 cggcgcaatc cccacttcaa aggaaacggt agctgtacgc ggcgtagcga aaagcggccc
    25681 caccgccagg taacaaaccg ttgccagcag cacgccagcg actttaccga ttggcgtgct
    25741 gaggctgtca acaccgccgc caacttttgc cagcgccact accgttaata ccggcaggcc
    25801 aacggcagta atgaggaagc cgaatgccgc agtccagacg tgttcgccag cctgcaagcc
    25861 gaccattgga gggaaaataa tgttacctgc gccgacgaac aacgcaaatg tcataaagcc
    25921 cagagcgatg atatcgcgcg atcttaattg atgggtcata aatcatactg cctgtggatg
    25981 tggtgttgta acgttaaatt tttgtgccaa tccctgggga tgatacagcg gtatatttaa
    26041 acaattttag cgggaaatac tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc
    26101 gcgttaatgc agtcagtatg acagcacctg aagcgcaatt taaacgctta taacgtttta
    26161 aagcaaggag ggatctaaaa accagaggta tatagcgata agaaatcacc aaccagtata
    26221 aaacactatt tccgtgaaaa agacatggct aatcatgcga acaaaaaatc accaaccgtt
    26281 gtaaaatcta acgcccgtat tttgactgca aagaaaataa gccagcatcg actggcttat
    26341 ggcaataaaa gatgacaaag gcggattaat cgctgttttt ggcaattaaa cgttccggga
    26401 taacaaaact gaaacgtgtt ccttttccta ctgtactctc aatattcagg cgactttcgt
    26461 gatgattcac agcatgtttc acgatcgcta accctaatcc gctaccgccg gtttgccggg
    26521 aacgcgcttt atcaacgcga taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa
    26581 tgcccggtcc gttatcttca acgctaaatt cggcaccgtg cggcactcgc tgccagcgta
    26641 cggtgatatg cgtgccttct ggagtatgat tcacggcgtt atagaccagg ttcgaaatcg
    26701 cactgcgtag ctgatcttcg ttgccagaca ctttgaggcc gttatctatc tcaaaggtaa
    26761 atgtctgttt tttctgactc agagtctgag cctcgcgctc aacaacgcgc agcatcatcg
    26821 gcacatcaac cttttcattg agcaattgcg tcggcgcggc ttcaattttc gacagcgtta
    26881 gcaactgctt caccagccct tccatccgct gggtctgctc gcgcatggtg tgcaacgctt
    26941 tttcgcgtac cgcgccttcc agcggctgct catccatcat ctccaggtaa ccctgtaaca
    27001 cggtcaatgg cgtacgtaac tcatggctca cgttggcaaa aaagttacgc cgcgcccctt
    27061 ccagttgatg catttgcgtg acatcacgcg ccaccatcag caactgtttg tgggtataag
    27121 gcatgacgcg aatttccaga tgccgcccgg tgttgagcac cagattgagc gggcgagaaa
    27181 aatcacgcgt tttcagatat tgcgtaaact ccgggtaacg cagtaggtta aggatgttct
    27241 gcccgttatc ttccggccag cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga
    27301 aaataccgct ctcttccgtg gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac
    27361 gaaagcgttt aatcagattg cccagttcac ggcggcgttt tttatttcgc agctgcatct
    27421 ggtgtaagcc gtatagtagc ggttcccagc taccacgccc cggtggcggg gtcatactgc
    27481 gatccaccca cagccaccat gaaaggcgca ataaattcca gaaatgccag ataagcagtc
    27541 ctgttaccga tgccagcaaa aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag
    27601 ccgggaggca gcaaagtagc agctccagca ccagcctttt ccacgacagc cgttccagca
    27661 cgtaagatac tcccgttaag aaatcataag ccctgctctg cgtccgatga gcaaggcgtt
    27721 aaaagcgggt tgaaaaacga tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc
    27781 cgccgggctc cagtgcttta cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca
    27841 catacacgtt agttccccag acgtggttta acagctgctc gcggctgtac acgcgctcag
    27901 gatgcgtcat aaaaaagtgc agcagtttaa attctgtcgg ccccatctcc agcggctctt
    27961 cgcccgccat cactcggtga gatgtcgggt cgagacttaa tccctgcatc tcaatcacct
    28021 cttccaccgc cattggcgaa atacggcgca ttaccgcttt gattcgcgcc accagctcct
    28081 tcggcgaaaa cggcttggtg atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat
    28141 cttcttcttc ccctctggcg gtcaacatca ccactggaat atcccgggtc atcgactcgc
    28201 gcttaaggtg tttgatgaac tggataccgg agccgccagg taacatccag tcaaggagaa
    28261 ttaaatccgg ccagggttca ttcagttgat tcacagcaca gtcataatct tccgcttcga
    28321 ccggctgaaa gccattttgt tcgagcacga agcagaccat ttcacgaatt ggagcttcat
    28381 cttctacgac cagaatacgt ctcgccatga tttgccctgt tgtaataaat aggttgcgat
    28441 cattaatgcg acgtcattat gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac
    28501 atatcttcag gttattgatt tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg
    28561 tataatcgcg cccatgcttt ttcgccaggg aaccgttatg cgcatccttc acacctcaga
    28621 ctggcatctc ggccagaact tctacagtaa aagccgcgaa gctgaacatc aggcttttct
    28681 tgactggctg ctggagacaa cacaaaccca tcaggtggat gcgattattg ttgccggtga
    28741 tgttttcgat accggctcgc cgcccagtta cgcccgcacg ttatacaacc gttttgttgt
    28801 caatttacag caaactggct gtcatctggt ggtactggca ggaaaccatg actcggtcgc
    28861 cacgctgaat gaatcgcgcg atatcatggc gttcctcaat actaccgtgg tcgccagcgc
    28921 cggacatgcg ccgcaaatct tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc
    28981 cattccgttt ttacgtccgc gtgacattat taccagccag gcggggctta acggtattga
    29041 aaaacagcag catttactgg cagcgattac cgattattac caacaacact atgccgatgc
    29101 ctgcaaactg cgcggcgatc agcctctgcc catcatcgcc acgggacatt taacgaccgt
    29161 gggggccagt aaaagtgacg ctgtgcgtga catttatatt ggcacgctgg acgcgtttcc
    29221 ggcacaaaac tttccaccag ccgactacat cgcgctcggg catattcacc gcgcacagat
    29281 tattggcggc atggaacatg ttcgctattg cggctccccc attccactga gttttgatga
    29341 atgcggtaag agtaaatatg tccatctggt gacattttca aacggcaaat tagagagcgt
    29401 ggaaaacctg aacgtaccgg taacgcaacc catggcagtg ctgaaaggcg atctggcgtc
    29461 gattaccgca cagctggaac agtggcgcga tgtatcgcag gagccaccag tctggctgga
    29521 tatcgaaatc actactgatg agtatctgca tgatattcag cgcaaaatcc aggcattaac
    29581 cgaatcattg cctgtcgaag tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt
    29641 gttagccagc caacagcgtg aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg
    29701 ccgtctggca ctggaagaac tggatgaatc gcagcagcaa cgtctgcagc atcttttcac
    29761 cacgacgttg cataccctcg ccggagaaca cgaagcatga aaattctcag cctgcgcctg
    29821 aaaaacctga actcattaaa aggcgaatgg aagattgatt tcacccgcga gccgttcgcc
    29881 agcaacgggc tgtttgctat taccggccca acaggtgcgg ggaaaaccac cctgctggac
    29941 gccatttgtc tggcgctgta tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa
    30001 aatgatctca tgacccgcga taccgccgaa tgtctggcgg aggtggagtt tgaagtgaaa
    30061 ggtgaagcgt accgtgcatt ctggagccag aatcgggcgc gtaaccaacc cgacggtaat
    30121 ttgcaggtgc cacgcgtaga gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa
    30181 gtgaaagata agctggaact gacagcgacg ttaaccgggc tggattacgg gcgcttcact
    30241 cgttcgatgc tgctttcgca ggggcaattt gctgccttcc tgaatgccaa acccaaagaa
    30301 cgcgcggaat tgctcgagga gttaaccggc actgaaatct acgggcaaat ctcggcgatg
    30361 gtttttgagc agcacaaatc ggcccgcaca gagctggaga agctgcaagc gcaggccagc
    30421 ggcgtcacgt tgctcacgcc ggaacaagtg caatcgctga cagcgagttt gcaggtactt
    30481 actgacgaag aaaaacagtt aattaccgcg cagcagcaag aacaacaatc gctaaactgg
    30541 ttaacgcgtc aggacgaatt gcagcaagaa gccagccgcc gtcagcaggc cttgcaacag
    30601 gcgttagccg aagaagaaaa agcgcaacct caactggcgg cgcttagtct ggcacaaccg
    30661 gcacgaaatc ttcgtccaca ctgggaacgc atcgcagaac acagcgcggc gctggcgcat
    30721 attcgccagc agattgaaga agtaaatact cgcttacaga gcacaatggc gcttcgcgcg
    30781 agcattcgcc accacgcggc gaagcagtca gcagaattac agcagcagca acaaagcctg
    30841 aatacctggt tacaggaaca cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg
    30901 cgtgcgcagt tctcccaaca aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag
    30961 ttaacccatg ctgagcaaaa acttaatgcg cttgcggcga tcacgttgac gttaaccgcc
    31021 gatgaagttg ctagcgccct ggcgcaacat tccgagcaac gcccactgcg tcagcgcctg
    31081 gtcgcgctgc atgggcagat tgttccccaa caaaaacgtc tggcgcagtt aatggtcact
    31141 atccagaatg tcactctgga acagacgcaa cgtaatgtcg cacttaacga aatgcgccag
    31201 cgttataaag aaaagacgca gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg
    31261 cgcatcaaaa cgctggaagc tcaacgtgca cagttacagg cgggtcaacc ttgtccactt
    31321 tgtggttcca ccagccaccc ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat
    31381 cagtctcgat tactggcgct ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg
    31441 ctgcgcgggc aactggatgc attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa
    31501 agcctccgac aagatgagca agcacttact caacaatggc aagccgtcac gaccagcctc
    31561 aatatcacct tgcagccaca ggacgatatt caaccgtggc tggatgcaca agatgagcac
    31621 gaacgccagc tgcggttact cagccaacga catgaattac aagggcagat tgccgcgcat
    31681 aatcagcaaa ttatccagta tcaacagcaa attgaacaac gccagcagca gctcttaacg
    31741 gcattgacgg gttatgcact gacattgcca caggaagatg aagaagagag ctggttggcg
    31801 acacgtcagc aagaagcgca gagctggcag caacgccaga acgaattaac cgcgctgcaa
    31861 aaccgtattc agcagctgac gccgattctg gaaacgttgc cgcaaagtga tgatctcccg
    31921 cacagcgaag aaactgtggc gctggataac tggcggcagg ttcatgaaca atgtctcgca
    31981 ttacacagcc agcagcagac gttacagcaa caggatgttc tggcggcgca aagtctgcaa
    32041 aaagcccagg cgcagtttga caccgcgcta caggccagcg tctttgacga tcagcaggcg
    32101 ttccttgcgg cgctaatgga tgaacaaaca ctaacgcagc tggaacagct caagcagaat
    32161 ctggaaaacc agcgccgtca ggcgcaaacg ctggtcacac agacagcaga aacgctggca
    32221 cagcatcaac aacaccgacc tgacgggttg gctctcactg tgacggtgga gcagattcag
    32281 caagagttag cgcaaactca ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt
    32341 cgccagcagc tgaagcagga tgcagataac cgtcagcaac aacaaacctt actgcagcaa
    32401 attgctcaaa tgacgcagca ggttgaggac tggggatatc tgaattcgct aataggttcc
    32461 aaagagggcg ataaattccg caagtttgcc caggggctga cgctggataa tttagtccat
    32521 ctcgctaatc agcaacttac ccggctgcac gggcgctatc tgttacagcg caaagccagc
    32581 gaggcgctgg aagtcgaggt tgttgatacc tggcaggcag atgcagtacg cgatacccgt
    32641 accctttccg gcggcgaaag tttcctcgtt agtctggcgc tggcgctggc gctttcggat
    32701 ctggtcagcc ataaaacacg tattgactcg ctgttccttg atgaaggttt tggcacgctg
    32761 gatagcgaaa cgctggatac cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa
    32821 accatcggtg tgattagcca cgttgaagcg atgaaagagc gtattcctgt gcagatcaaa
    32881 gtgaagaaga tcaacggcct gggctacagc aaactggaaa gtacgtttgc agtgaaataa
    32941 ctattcagca ggataatgaa tacagagggg cgaattatct cttggccttg ctggtcgtta
    33001 tcctgcaagc tatcacttta ttggctacgg tgattggtag ccgttctggt ggttgtgatg
    33061 gtggtatgaa aaaagtcatt ttatctttgg ctctgggcac gtttggtttg gggatggccg
    33121 aatttggcat tatgggcgtg ctcacggagc tggcgcataa cgtaggaatt tcgattcctg
    33181 ctgccgggca tatgatctcg tattatgcac tgggggtggt ggtcggtgcg ccaatcatcg
    33241 cactcttttc cagccgctac tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg
    33301 tcattggcaa cgccatgttc acgctctctt cgtcttacct gatgcttgcc attggtcgcc
    33361 tggtttccgg ctttccgcat ggcgcatttt ttggcgtcgg cgcgatcgtg ttatcaaaaa
    33421 ttatcaaacc cggaaaagtc accgccgccg tggcggggat ggtttccggg atgacagtcg
    33481 ccaatttgct gggcattccg ctgggaacgt atttaagtca ggaatttagc tggcgttaca
    33541 cctttttatt gatcgctgtt tttaatattg cggtgatggc atcggtctat ttttgggtgc
    33601 cagatattcg cgacgaggcg aaaggaaatc tgcgcgaaca atttcacttt ttgcgcagcc
    33661 cggccccgtg gttaattttc gccgccacga tgtttggcaa cgcaggtgtc tttgcctggt
    33721 tcagctacgt aaagccatac atgatgttta tttccgtttt ttcggaaacg gcgatgacct
    33781 ttattatgat gttagttggg ctagggatgg tgctgggaaa tatgctaagt ggcaggattt
    33841 caggacgtta ttcaccactg cgcattgcag cagtgactga ctttatcatt gtactggcac
    33901 tgctgatgct ctttttctgc ggcggcatga aaacaacgtc gcttattttt gcttttattt
    33961 gttgcgcggg attatttgcc ctttcagcac ctctgcaaat attgttactg caaaacgcca
    34021 aaggcggaga gttattaggt gccgcaggtg ggcaaatagc gtttaacctc ggtagcgccg
    34081 tcggcgcata ttgcggaggt atgatgctga cgctggggct ggcatataat tacgtggcgc
    34141 tgcctgccgc cctgctttcg tttgctgcga tgtcatcgtt gctgctgtat ggtcgctata
    34201 agcgccagca agcggcggat agtccggtgc tggcgaaacc actggggtag attactgtca
    34261 cggtgcgcgt tgcttattgc cggatgcggc gtgaacgcct tatccgccct acgcggttct
    34321 ggcacatttt gtaggcctga taagacgcgg caagcgtcgc atcaggcatg atgcgccaat
    34381 tgcctacgtt ttttactctt gtggccataa ccacgcagcg ccgcgtacgc cgctggaatc
    34441 accgtgcttc gccttacgca ccggcgtttc acattcgccg ccgaagacaa attgtttaat
    34501 caactgccca accgtttgat ataaacggtc tacattgctc atcccgcccc ccaggacaat
    34561 cacatccgga tcgagaatat tcacgacatg tgccagcgat tttgccagcc gcagctcgta
    34621 gcgacgcaat gccagttccg ctaccggatc gctttcttca accaggcgga taatttcact
    34681 gcctttcagc gcatgtccgc tcaaacgacg ataatccgtc gcgaatcccg tgcccgaaat
    34741 aaaggtttca atacaacctt gtttaccgca ataacaaggg acttcctcgc gataacgcag
    34801 ctcgtcttca tccatccacg gtagcggatt gtgtccccac tcgcctgccg tgccattgcc
    34861 gccgatatgc gcccgcccat ttaatgccac gcccgcgccg catcccgtgc cgataatcac
    34921 ggcaaatacc gtctgcgctc ccgctgccgc gccatctact gcttctgaaa ccgccagaca
    34981 gttggcatca tttgccagcc gcacttcccg ctgcaacctc gcgcttaagt ctttatcgaa
    35041 tggctgaccg ttgagccagg ttgaattggc attcttcacc acaccggtgt agggcgaaat
    35101 cgaacctgga atgcccatac ctaccgttcc gcgctgccct gtcgcctgct ccgccatatc
    35161 aaccaacgtg gcgatcgttt caatagtctg ccggtaatca tcacgcggcg tgggcagacg
    35221 atggcggtac aactgctccc ctgcatcgcc cagtgcaatc acttcagttt tggtgccgcc
    35281 taaatcgata cctatacgca ctgtactctc cttatttttt tcaatatcaa tagcgtagag
    35341 acggacaacc ggattggcaa tgcaaggccg ccgacaattc gttatcatgc ccgctaaatt
    35401 taacgacaag gccgtggaaa ttatcatgct gtggttcaaa aatttaatgg tttaccgtct
    35461 tagccgcgag atttcgctgc gtgcagaaga gatggaaaaa cagctagcct cgatggcatt
    35521 taccccatgc ggcagccagg acatggcgaa gatgggctgg gttcctccga tgggatcgca
    35581 cagcgatgcg ttaacgcacg ttgccaatgg tcaaattgtt atctgcgcgc gcaaagaaga
    35641 aaaaatcctc ccgtctccgg tgattaaaca ggcgctggaa gcgaaaatcg ccaaactgga
    35701 agcggaacag gcgcgtaagc tgaagaaaac cgaaaaagat tcgctgaaag acgaagtgct
    35761 gcactctctg ctgccgcgtg ctttcagccg ttttagccag acaatgatgt ggatcgacac
    35821 ggttaacggt ttgattatgg tggactgcgc cagtgccaaa aaagcggaag atacgctggc
    35881 attactgcgt aaaagcctgg ggtcgttacc ggttgtacca ttgagcatgg aaaacccgat
    35941 tgaactgacg ctgaccgaat gggttcgctc cggtagtgcg gcacagggct tccagctgct
    36001 tgatgaggcc gagctgaaat cgttgctgga agatggcggc gtgatccgcg cgaagaaaca
    36061 agatctgacc agcgaagaga tcaccaatca cattgaagcc ggaaaagtgg tgactaaact
    36121 ggcgctcgac tggcagcagc gcattcagtt tgtgatgtgc gacgatggtt cgctcaagcg
    36181 tcttaagttc tgcgacgagc tgcgcgatca aaacgaagat atcgaccgtg aagatttcgc
    36241 cctgcgtttt gatgccgatt tcatcctgat gactggtgaa ctggcagcgt taattcaaaa
    36301 cctgattgaa ggattaggtg gcgaagcaca acgttaattg ctgattttcc tttaatgccg
    36361 gatgcgacgc ctgccgcgtc ttatccggcg tacgaagcca caccaggcat ataattattc
    36421 gctacggcga gcaataattt ttagcgcagc aatattatgc gttttacgcc gtaactgatc
    36481 taatcgcctc atccctattc gcctgtcagc agcacgcaga atattcacta acccccattt
    36541 atcgctggta aattgttttg ttaacagctc cgcttttggg gtatcaatat attcacgcaa
    36601 taaatcagaa attgaacaaa tatccgtcag gtaaggatac cagcaagttt caccgtgaaa
    36661 attacgcact ccagcgttac ctgcaataaa gtctttcggg taatcccaga tcacgttttt
    36721 atccaacgtg atccaataac gcggtaaatc ggtacttcca ccattttggc tgcgcatgag
    36781 ataacgggta cagtgaattt gcaggttgat cgttggcgtc agtaagtcat agatctcccg
    36841 ctgtaacttg ctccatggat gttgtgtcat tgtttttcct caagccggtg acgcgcttca
    36901 ctgcgaattt tccgcgttgc cccggttttc actaaatcac gtaactgcga aagatcgggt
    36961 tgttcgacaa ataagagtaa tgatagatcc tcttttaacc acgccgtttt cacatcggca
    37021 gccagctgtg gattattaat agccagcgac cgatcctgag actcagtcaa tgtcgttaac
    37081 aacgacgccg gggtatgtgc gttgcgtaat gccgtttggc gcaaatgatc attgcgactt
    37141 tgcagcaatg cacgccacca ttcatcatct gatttatctt ttatcgtagc cagttccgca
    37201 aaaaatacct ggagatcttc actgatattt atatcaacct tgatgaatta tgaatatatc
    37261 ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAJTYZ010000027        36807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000027 DAJTYZ010000000
VERSION     DAJTYZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.027"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..192)
                     /locus_tag="OO596_001309"
     CDS             complement(<1..192)
                     /locus_tag="OO596_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717211.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSER"
     gene            complement(292..1203)
                     /gene="gsiD"
                     /locus_tag="OO596_001310"
     CDS             complement(292..1203)
                     /gene="gsiD"
                     /locus_tag="OO596_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6717212.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1206..2126)
                     /gene="gsiC"
                     /locus_tag="OO596_001311"
     CDS             complement(1206..2126)
                     /gene="gsiC"
                     /locus_tag="OO596_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6717213.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2144..3682)
                     /gene="gsiB"
                     /locus_tag="OO596_001312"
     CDS             complement(2144..3682)
                     /gene="gsiB"
                     /locus_tag="OO596_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6717214.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3702..5573)
                     /gene="gsiA"
                     /locus_tag="OO596_001313"
     CDS             complement(3702..5573)
                     /gene="gsiA"
                     /locus_tag="OO596_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6717215.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5560..6525)
                     /gene="iaaA"
                     /locus_tag="OO596_001314"
     CDS             complement(5560..6525)
                     /gene="iaaA"
                     /locus_tag="OO596_001314"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6717216.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6729..7964
                     /gene="moeA"
                     /locus_tag="OO596_001315"
     CDS             6729..7964
                     /gene="moeA"
                     /locus_tag="OO596_001315"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6717217.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7964..8713
                     /gene="moeB"
                     /locus_tag="OO596_001316"
     CDS             7964..8713
                     /gene="moeB"
                     /locus_tag="OO596_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6717218.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8892..9554)
                     /gene="fsa"
                     /locus_tag="OO596_001317"
     CDS             complement(8892..9554)
                     /gene="fsa"
                     /locus_tag="OO596_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6717219.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9685..10584
                     /locus_tag="OO596_001318"
     CDS             9685..10584
                     /locus_tag="OO596_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6717220.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10590..13022
                     /locus_tag="OO596_001319"
     CDS             10590..13022
                     /locus_tag="OO596_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6717221.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13168..13983
                     /gene="ybiV"
                     /locus_tag="OO596_001320"
     CDS             13168..13983
                     /gene="ybiV"
                     /locus_tag="OO596_001320"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6717222.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14135..15400
                     /locus_tag="OO596_001321"
     CDS             14135..15400
                     /locus_tag="OO596_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6717223.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15367..16527)
                     /locus_tag="OO596_001322"
     CDS             complement(15367..16527)
                     /locus_tag="OO596_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6717224.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            16765..18066
                     /locus_tag="OO596_001323"
     CDS             16765..18066
                     /locus_tag="OO596_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6717225.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18081..20444
                     /locus_tag="OO596_001324"
     CDS             18081..20444
                     /locus_tag="OO596_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCR6717226.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(20624..22219)
                     /locus_tag="OO596_001325"
     CDS             complement(20624..22219)
                     /locus_tag="OO596_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6717227.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22438..23358
                     /gene="ldtB"
                     /locus_tag="OO596_001326"
     CDS             22438..23358
                     /gene="ldtB"
                     /locus_tag="OO596_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6717228.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23417..24535)
                     /locus_tag="OO596_001327"
     CDS             complement(23417..24535)
                     /locus_tag="OO596_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6717229.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24532..24999)
                     /gene="mntR"
                     /locus_tag="OO596_001328"
     CDS             complement(24532..24999)
                     /gene="mntR"
                     /locus_tag="OO596_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6717230.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25185..25313
                     /gene="mntS"
                     /locus_tag="OO596_001329"
     CDS             25185..25313
                     /gene="mntS"
                     /locus_tag="OO596_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6717231.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25585..27168
                     /gene="opgE"
                     /locus_tag="OO596_001330"
     CDS             25585..27168
                     /gene="opgE"
                     /locus_tag="OO596_001330"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6717232.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27217..27732)
                     /gene="ompX"
                     /locus_tag="OO596_001331"
     CDS             complement(27217..27732)
                     /gene="ompX"
                     /locus_tag="OO596_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6717233.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(27785..27850)
                     /locus_tag="OO596_001332"
     CDS             complement(27785..27850)
                     /locus_tag="OO596_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717234.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28085..28972
                     /gene="rhtA"
                     /locus_tag="OO596_001333"
     CDS             28085..28972
                     /gene="rhtA"
                     /locus_tag="OO596_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6717235.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29271..29774
                     /gene="dps"
                     /locus_tag="OO596_001334"
                     /gene_synonym="pexB"
     CDS             29271..29774
                     /gene="dps"
                     /locus_tag="OO596_001334"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6717236.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30178..30924
                     /gene="glnH"
                     /locus_tag="OO596_001335"
     CDS             30178..30924
                     /gene="glnH"
                     /locus_tag="OO596_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6717237.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31063..31722
                     /gene="glnP"
                     /locus_tag="OO596_001336"
     CDS             31063..31722
                     /gene="glnP"
                     /locus_tag="OO596_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6717238.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            31719..32441
                     /gene="glnQ"
                     /locus_tag="OO596_001337"
     CDS             31719..32441
                     /gene="glnQ"
                     /locus_tag="OO596_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6717239.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            32558..34783
                     /gene="ybiO"
                     /locus_tag="OO596_001338"
     CDS             32558..34783
                     /gene="ybiO"
                     /locus_tag="OO596_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR6717240.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(34780..35706)
                     /gene="rlmF"
                     /locus_tag="OO596_001339"
     CDS             complement(34780..35706)
                     /gene="rlmF"
                     /locus_tag="OO596_001339"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6717241.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            35982..36242
                     /locus_tag="OO596_001340"
     CDS             35982..36242
                     /locus_tag="OO596_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6717242.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36507..>36807
                     /locus_tag="OO596_001341"
     CDS             36507..>36807
                     /locus_tag="OO596_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6717243.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         8903 a         9956 c         9253 g         8695 t
ORIGIN      
        1 gcgttccgat accagttgat agttaatgat gaaaaaaaga ccaataaatg acagtgctgc
       61 caataaaaat aaaattatca gccttatctt tatcttttcg tataaactca gcatgttaat
      121 acatttaaca aaataactat ctgaacgctg tgccgctgca aaaatagtcc cttgctgcgg
      181 ctttttaacc aaattacccg attctacaaa atcagcagaa agttacaaca cttgcttagg
      241 gcaataattt cctgtgaggt gattaccctt tcaagcaata ttcaaacgta actatccttt
      301 aattttcgga tccagcgcat cgcgtaaacc atcgcccaac aaattgaacg ccagtacggt
      361 cagaaaaata gccagggccg gaaaaacagc gacatgcggc gcgataacca tatccgctcg
      421 tgcctcattg agcattgctc cccactctgg tgtcggcggc tgcgcaccga ggccgagaaa
      481 tgacaggctg gcggcagaga taatcgaggt gccaatgcgc atggtgaaaa acaccacgat
      541 agacgagacg gtcccaggca agatatgacg caataaaatg gtcatatcgc tggcaccaat
      601 actgcgtgct gactcaataa aggtttgctg tttcaacacc agcgtgttgc cgcgcaccag
      661 tcgggcaaac gcggggatgg aaaaaatggc gactgcgata atcacgttag caatgccgct
      721 tcccaacacc gcaacaacag cgatcgccag taaaataccc gggaaggcaa acagcacatc
      781 gcaaatgcgc atgatcagcc gatcccacca gccttcataa tatccagcca acaagcccag
      841 caacgtcccg atcgccgcac cgataaacac ggcaaacacg cccgccgcca gcgagatttg
      901 cgcaccaacc aggacacggc tgaaaatgtc gcgccccagt gaatcgacgc caaaccagtg
      961 ctgcaaagaa ggtccgttat tcagattgtc ataatcaaaa taattttcgg cgtcataggg
     1021 agcgatccag cgtgcaaaaa tggccaccac aatcaataaa ataacgaata atgcggcggt
     1081 catcgccata tgctgacggc gaaatcgtcg ccagaattca tgccacggtg tacgcacctg
     1141 gtcaggtttg accagtggca tggcgtttaa caccgcctga cgtcgccagt taaatagtcg
     1201 catccttact tgtacctgat agccgggtta atggcggcgt aaagcacatc caccactaag
     1261 ttgataagaa taaattccag cgagaaaagc agaatttccg cctgaatcac cgggtaatcg
     1321 cgcatttcta cggagtcaac gagtaagcgt ccaagtccgg gccagttgaa aactttctcc
     1381 accacgatag aaccaccgag caaaaagcca aactgtaagc ccatcatggt cactaccggg
     1441 atcatcgcgt tacgtagccc gtgtttgagg acaacccagg tttcgctcac ccctttcgcc
     1501 ctcgcggtac gcatataatc ttcgcttaaa acatcgacaa acgacgcgcg ggtaaagcgc
     1561 gccatcacgg ctgccactgc cgcgccgagc gtcagggagg gtaaaatgta gtgctgccaa
     1621 ctgtctgctc ccacggtagg cagccagccc agttcaacgg agaatacctg aattaaaagc
     1681 atccccagcg caaatgccgg aaacgagatc cccgacaccg caatggtcat actcaatcga
     1741 tccggccaac gattacgcca gacggcggcg ataattcccg ccgccatacc aaatatcacc
     1801 gcccagacca tactggttat ggtcagccac agcgttggca taaagcggct ggcaatctca
     1861 tcggcaaccg gacgacgcga caccatcgac aggccaaaat ccccctgcac agcattgctg
     1921 atatagtgcc agaactggtg atacagcggc tgatccaacc ccagctgctg acgcaccagt
     1981 tctataacct gcgcatcagc ttcgagcccg gcaatcaatc gcgccggatc gccgggcagc
     2041 atatgcacaa ataaaaacac cagcaccgag acgataaaca gcgtcggaat caaccccagt
     2101 aagcgtttga taacgtaatt aagcattcca ctccctgcgt tgcttattgc aaatccgcgt
     2161 cttcaaagct gaagccggtg tctggcatga tccaaaaacc ggtcaggttt ttactgtgtg
     2221 ccgacaccag tttttctacc accagcggga tccacggcga ctcttgccag atgatatcct
     2281 gcgccgcctt atacaagcgg gtcttttccg ccggatcatt agttttcagt gcctgagcca
     2341 ggaagtcgtc cacctgttta ttgctgtaaa atgcggtatt aaacagcgtc ggtggccagt
     2401 tttgcgaggc gaacagtggc gatagcgccc agtccgcttc gccggttgaa gccgaccagc
     2461 cagtgtagaa catccgcacg ccgctctctt tttgcccttt accttcaact tctgccgccc
     2521 gctgtccggc atccatcgcg gtcacctggg ctttaatccc gacctgcgct aactgctgct
     2581 gggtaaattg cagcactttc tgcgcggtgc tgtggttatg tgacgaccac agcgtggtac
     2641 tgaaaccgtt gggatatccc gcctctttca gtaattcgcg cgctttcact ggatcgtaag
     2701 gccacggttt ataactttgc gcgtaggcga tacttggcgg taccacacca gtagctggcg
     2761 ttgcatagcc cgcaaaggca actttcacca gcgccggacg gttaatggcg taattcagcg
     2821 cctcacggac cttcgggtta tcgaacggct tttgcgtcac gttcatactg atataacgct
     2881 gcataattga cggactggcc atcaactcga tatttttgtt tttctccagc agtgtggcct
     2941 gctcgtaggg aatggggaaa gcaaactgcg cttcaccggt ttgcagcatt gccgcgcggg
     3001 tgttgttatc cgccaccgga cgccaggtta tgctgtccag tttgggcaat cctggctgcc
     3061 agtaacccgc gaattttttc accttcacaa aatcggtctg attccaggta tccagttcat
     3121 acggtccggt tcccaccgga tgaaaaccaa tctccttgcc atatttttcc agcgctgccg
     3181 gtgaaatcat cgcggtcgcc ggatgggcaa gaatattaat aaacgctgag aacggctgtt
     3241 tgagggtaat ctttaccgtt gtcggatcga tcgcctccgt tttggcaata ttcttataca
     3301 ggttatagcg tttaagatga ttcgccggat cgctggcccg gtccagattc gcttttaccg
     3361 ccactgcgtt gaaatcggtg ccatcctgga atttaatgcc ttcccgcaat ttcacggtgt
     3421 aagtaatgcc gtcatcggaa acggtataac tctccgccag cacgtttttc agtttcatct
     3481 ctttatccag accgaacagc ccctggtaaa acgatttcgc tacggcctga gataacgtat
     3541 cattcgcgtc atacggatcg agcgtggtga aattcgatcc taccgccacc accacatctt
     3601 tggcagcgaa tgcacaagat gccatcaacg ctgtcgcaat gcccagcgcc actaacccac
     3661 tacggtgtac agctcttgcc attttattct ccgcctgaat gttatctacg catgaatgcg
     3721 tattctgatt gtggttgtgc gacgtaatgc cccggcccga cgcattgcaa cgagacggct
     3781 gccacctctt cgccacgcag atgaatattg ctgggaagat cgtccgacag cagtacacgc
     3841 tgcggtcgtt gtcgggacgg ttcagcgacc ggaactgccg ccagtaattt acgcgtataa
     3901 ggatgctgcg ggttttcgaa gaccgcgcgc cgtggaccaa tttcaacaat ttgcccgaga
     3961 tacatcaccg ccacacgatg actaatccgc tctaccacgg ccatatcgtg ggagataaac
     4021 agatacgcaa tgccgaaatc acgctggaga tcgagcaaca agttgataat ctgcccgcga
     4081 atagaaacat ccagcgccga aacggcttcg tcggcaatga tcacttttgg attcaatgcc
     4141 aacgcgcgag caatgcagat gcgctggcgc tgaccgccgg aaaactcatg cgggtaacgc
     4201 caggcatgtt caggtaacag gcccacgcgc tccagcaacc acgcaacgcg tgcagccgcg
     4261 tctttacctg gcaataaacc gtgtacacgc agcggttcga taatcgaatc accgatggtc
     4321 tgacgtgggt ccagcgaagc gtaagggtcc tgaaaaataa actgaatatc ccggcgcaat
     4381 gcctgaagtt tgccgggtga caaggtatcg attctctgac cgttaaagat aatttcgccg
     4441 ccctgcgatt cgaccaggcg cagcaacgcc cgcccggtag tggatttacc gctgccagac
     4501 tcgcccacca gcgatagcgt ttcgccaggc cagagatcaa aactgacttt ctcaacggca
     4561 tgcacttccc gcgttacgcg attcaacaaa ccgctgcgca aagggaaacg ggtgacaaga
     4621 ttacgcactc gtaaaacagg ttcgccatcc accaccgttt tctgctcgat ggggggggcc
     4681 tgtttcgctg gatgttcaag cgatatcaac gggaaacgtc ggggataatc caaccctttc
     4741 atcgcaccaa gttgcggaac agcagctaac agcgcacggg tgtaaggatg ttgcggtgca
     4801 tgaaaaatct gttcgacggt acccgtttcc accgcctcgc cctgatacat caccagtacc
     4861 cgatcggcaa tctctgccac cacgcccata tcgtgagtga taaagataac gcccatcgac
     4921 atctcttttt gcaatacttt gattaattgc aggatctgcg cctgaatagt gacatccagc
     4981 gcggtggttg gctcatcggc aatcagcacc gccgggcggc atgacagcgc catcgcaatc
     5041 atcactcgct ggcgcatccc gccagagagt tgatgcggat aacgtgaaag aatggtttgt
     5101 gcctcaggaa tgcgtacctg atccagcatc cgcttcgcct cgaccatcgc ttcttcacga
     5161 ctggcgttct gatgcagacg aattgattcg gcaatctgtt cacccacagt aaataccggg
     5221 ttcagcgatg tcatcggctc ctgaaatatc atcgccatat ccgcaccgcg aacatggcgc
     5281 atttgtgcag cgctctgctc gctaagttca atcacttcgc gactgcgccg ctgcaacagc
     5341 attttatcgc actgtactaa accgcccgcc tgttccaaca ggcgcatcaa tgccaacgca
     5401 gtcactgact taccggagcc ggattcgcca acaattgcca gcgtctcacc gcgttgcaga
     5461 ctaaaagaga gattgcggac cgcagctatt ttctgctggt cctgcataaa ggcaatattc
     5521 agattttcaa ccgccagcac attaccggca tcaagttcat cactgtgtgg caacggtgtc
     5581 ccctttttca cggtagatac cggtggttgg cgtatcgcct gcgtagcccc aggcgcgata
     5641 cattccttcg gtgttaaacg gtagcgcgac attcccttca tggtcgatag cgattaagcc
     5701 accgctaccg ccaagcgcag ggagtttttc cattactacc cgctcgcagg cttccgcgag
     5761 acttaatccg ccgtaatcca ttaacgcggc gatgtcatat gccgccagcg cgcggatgaa
     5821 gacttcgccc gtgccgatac aagaaaccgc cacactggcg ttattggcgt agcatccggc
     5881 acccactaag ggactatcgc caactcgtcc gggtaattta ttggtcattc cgcctgtgga
     5941 cgtggctgcc gccaaattgc cgtctaaatc caacgccacg gcccccacgg tgcccatttt
     6001 ctgtttttca tccagtggcg caccgctatg gtcgaggacg gttgcccctt ccttgcgcgc
     6061 tgccagtagt tgttcataac gcaaaggcgt ggagaaaatc tccggcgaga cgcgctccat
     6121 gccacgagca aacgcaaaat tttctgcccc ttcgccaatc atcatcacat gcgggctttg
     6181 ctccatcacc agccgggcgg caagaaccgg attacgcaga tgactaacgc ccgccaccgc
     6241 accggctttc agggtgttac catccatcac acaggcgtcc agttcatggg tttcatcacg
     6301 cgtaaagaca gcgccaattc cggcgttaaa cagtggacac tcttccagca gacgcaccgc
     6361 ttccgtcacc acatccagcg cactttcgcc cgcttccagc attttctgcc cggtttcaac
     6421 aatggcagac aacgcctcaa tgtagcgtaa ttcctgttgc agactcatct gcgcgcggct
     6481 aattgcacct gcgccaccat gaattgcaat gactgctttg cccatagtat tcgtccatta
     6541 agggtggctc gccacttttg ctataaatat cagaattttt atgaattact cattcaattt
     6601 ttgaatatag aaagatggat atggcatgta aaggcaggag ccaacgctac gcccgaggtg
     6661 gcatacagtc tgtggcgttt ttctgacata ataggcaaat tcgattttgc ctccgcagga
     6721 gtgttttcat ggaatttacc accggattga tgtcgctcga caccgcgctt aatgagatgc
     6781 tttctcgcgt caccccactg accgcccagg aaacgctgcc actggtacag tgttttggtc
     6841 gtattctggc gagcgatgtc gtttcgccgc ttgatgttcc ggggtttgat aactccgcaa
     6901 tggacggcta cgcggtgcgt ttagccgata ttgcctccgg gcaaccgctg cccgttgccg
     6961 gtaaatcctt tgccggtcag ccgtatcatg gtgaatggcc tgcgggcacc tgcattcgta
     7021 ttatgaccgg tgcgccggtg ccggaaggct gcgaagcggt ggtgatgcag gagcagactg
     7081 aacaaacgga caatggcgtg cgttttactg ctgaagtgcg tagcgggcaa aatattcgcc
     7141 gtcgcggtga agatatctct gcaggtgcgg ttgttttccc ggcgggaact cgcctgacta
     7201 ccgcagagct gccagtaatt gcttcactgg ggattgccga agttccggtg attcgtaaag
     7261 tgcgtgtagc gcttttttct accggtgatg aactccagtt gcccggtcag ccgctgggcg
     7321 acggccaaat ctacgacacc aaccgtctcg ccgtacacct gatgttagaa cagctgggtt
     7381 gcgaggtaat caacttaggg attatccgcg acgatcccca tgccctgcgc gccgcattta
     7441 ttgaagccga cagccaggcg gatgtagtga tcagttccgg cggtgtttca gtgggtgaag
     7501 cggattacac caaaacgatt cttgaagagc tgggggagat cgccttctgg aagctggcga
     7561 ttaaaccagg taaaccgttc gcgttcggta aactcagcaa tagctggttc tgcggcctgc
     7621 cgggcaaccc ggtttcagcg acgctgacct tctatcaact ggtacagcct ttgctggcaa
     7681 aactaagcgg caacaccgcc agcggcctgc ccgcgcgcca gcgcgtacgc acagcgtccc
     7741 gcctgaaaaa aacgccagga cgtcttgatt tccagcgcgg cgtgctgcaa cgcaacgccg
     7801 acggtgaact tgaagtgacg accaccggac atcagggttc acatatattt agctccttta
     7861 gtctcggcaa ctgctttatc gtgctggaac gcgatcgcgg caatgtggac gtgggcgaat
     7921 gggtggaagt agaaccgttt aacgcgttgt tcggaggcct gtaatggcgg aactcagcga
     7981 tcaggagatg ctgcgctaca accggcaaat cattctgcgc ggttttgatt ttgacggtca
     8041 ggaggcgcta aaagattctc gtgtgctgat cgtcggcctc ggtggactcg gctgcgcagc
     8101 ttcgctgtat ctggcaagcg ccggtgtcgg caacctgacg ctgctcgact tcgacacggt
     8161 ttcgctctcg aatctgcaac gccagacact gcacagtgat gccacggtcg ggcaaccgaa
     8221 ggtggaatcc gcccgtgacg ccctgacgcg gatcaaccca catatcgcga ttacgccagt
     8281 caatgcactg ctagatgacg cagaacttgc agcattgatt gctgaacacg atctggtgct
     8341 cgactgtacg gataacgttg cggtacgtaa tcaactgaac gcaggctgtt ttgccgcgaa
     8401 ggtgccgctg gtttccggcg cggcaattcg tatggaaggg caaatcacgg tcttcactta
     8461 tcaggacggc gaaccgtgct atcgctgcct tagccgtttg tttggcgaga acgcattaac
     8521 ctgcgtagaa gcaggcgtga tggcaccgtt gatcggcgta attggttcgt tgcaggcaat
     8581 ggaagcgatc aaactgctgg caggttatgg caaaccgacc agcgggaaaa tcgtcatgta
     8641 cgatgcgatg acctgtcagt ttcgcgaaat gaaactgatg cgcaatccgg ggtgtgaggt
     8701 gtgcgggcag taattgccac ctccaggcct gataagacgc gacaagcgtc gcatcaggca
     8761 tttgctccag acgccagatg cggcgtgaac gccttatctg gcctacggtt cgggcacaga
     8821 tttccaagcg cggcaggcat cggtgcactc cgccacgtca tcagcaattt atgaggatgc
     8881 agaacgtccg gttaaatcga cgttctgcca aacgctccct gccagtcctg ctcaaacttc
     8941 gccacagcgg cttccaccgc cggatagcta atcatctgtt gtgccacatc cagtggcaga
     9001 gtaattgatt cacatcctgc cagtaagcag tccagcgcct gacgcggggt tttgaaactc
     9061 gctgccagca ctttcgcctg cggcgcatgc attttcaata actggtgtaa gtcggtcaca
     9121 gtctgaatgc cgctaccgcc ctgagcatca atacgattaa cgtaaggcgc aacatattcc
     9181 gcacctgcca gcgccgacag caacccttgt gctgcgccat ataccgcggt tcccagcgtt
     9241 ggaatccctt ccgcttttaa catcttaata gctgccagcc cctcggcggt caccggaact
     9301 ttcaccacga tatccgcaat aatagaacgc aatttacgtg cgtcattcac catcccttca
     9361 gcagtggtag ccattacctg ggcaaacaga cgcccctgac cgcccatcgc ttcatgaagt
     9421 tgcggaagca caacatccag cggtttttta cccgcggcga taatgcttgg gttagtggtc
     9481 acacccgcca gcggaaaaat acgtgacagc gccttcaccg caacaacgtc tgaagtatcc
     9541 agatacagtt ccataaccat cctcaaaatg tactttttgt ctgaatgtag actatcacgg
     9601 gaaaactctc tgaacagttg acgcacatca atctaacttt cattcgaaag tattttaatg
     9661 tttgaacgaa acagagaggc gactatgatt ttcaatattc agcgctactc gacccatgat
     9721 ggccccggta tccgcacggt cgtatttctt aaaggctgtt cgctgggctg ccgctggtgt
     9781 cagaacccgg aaagccgcgc ccgcacgcag gatctgctgt atgacgcacg actgtgtctg
     9841 gaaggctgcg agctgtgcgc taaggccgcg ccggaagtga ttgagcgcgc gctgaatggt
     9901 ttgcttattc atcgggaaaa gttaaccccg gagcatctga cggcgttaac cgactgctgt
     9961 ccgacacagg cattaaccgt gtgtggtgaa gtgaaaagcg ttgaggagat catgacgacc
    10021 gttctgcgcg ataaaccgtt ttacgatcgc agcggcggcg gtttaacgct ttcgggtggt
    10081 gagcccttta tgcagccgga aatggcgatg gcgctactgc aagccagcca cgaggcaggc
    10141 attcatactg cggtagaaac ctgtctgcat gtgccgtgga aatatatcgc cccttctctg
    10201 ccctatatcg atctgtttct tgccgattta aaacacgttg ccgacgcgcc gtttaaacag
    10261 tggaccgacg gtaacgccgc cagagtgctg gataacctga aaaaactcgc cgcagcgggc
    10321 aaaaaaatca ttatccgcgt gccgctgatt cagggcttta atgccgacga aacctctgta
    10381 aaagctatta ccgattttgc cgccgacgag ctgcacgttg gcgaaattca tttcctgccc
    10441 taccacacgc taggcatcaa caaatatcac ttacttaatc tgccctatga cgccccggaa
    10501 aaaccgcttg atgcgccaga actgctcgac tttgcccagc agtatgcctg ccagaaaggg
    10561 ttaaccgcga ccttacgagg ataacaacca tgaccacact gaaactggac acgctcagcg
    10621 accgcattaa agcgcacaaa aatgcgctgg tgcatattgt gaaaccgcca gtctgtaccg
    10681 agcgcgcgca gcactatacc gagatgtatc aacaacatct cgataagccg atcccggtac
    10741 gtcgcgcgct ggcactggcg catcacctgg cgaatcgcac catctggatc aaacacgatg
    10801 agttgatcat tggcaaccag gcaagcgaag ttcgcgccgc gccgatcttc ccggaatata
    10861 ctgtctcgtg gatcgaaaaa gagattgatg atctggcaga tcgtcccggt gctggctttg
    10921 cggtgagcga agagaacaaa cgcgttctgc atgaagtgtg cccgtggtgg cgcggtcaga
    10981 ccgtacagga tcgctgctac ggcatgttta ccgatgagca aaaaggtctg ctggcgaccg
    11041 gaatcattaa agcggaaggc aatatgacct ccggcgatgc gcacctggcg gtgaatttcc
    11101 cgctgctgct ggaaaaaggt cttgatggtc tgcgcgagaa agtagcggaa cgtcgctcgc
    11161 gcatcaatct gacggtactg gaagatttgc acggcgaaca gttcctgaaa gcgattgata
    11221 tcgtgctggt ggcagtcagc gaacacattg aacgttttgc agccctggcg cgtgaaatgg
    11281 ccgcgaccga aacccgcgaa agccgtcgcg atgaactgct ggcgatagca gaaaactgcg
    11341 atcttatcgc ccaccagccg ccgcagactt tctggcaggc gctgcaactg tgttacttca
    11401 tccaattgat attgcagatc gaatctaacg gtcactcggt atcgtttggt cgtatggacc
    11461 agtatctcta cccgtactat cgccgcgacg ttgaactcaa ccagacactg gatcgcgaac
    11521 acgccatcga attgctgcat agctgctggc tgaaactgct ggaagtgaac aagatccgct
    11581 ccggctcaca ctcaaaagcc tctgcgggaa gtcctctgta tcagaacgtc accattggcg
    11641 gacaaactct ggttgatggt caaccaatgg acgcggtgaa tccactctct tacgctatcc
    11701 tcgaatcctg cggtcgcctg cgttccactc agcctaacct cagcgtgcgt taccatgcag
    11761 gaatgagtaa tgatttcctc gacgcctgcg tacaggtgat ccgttgcggc ttcgggatgc
    11821 cggcgttcaa caacgacgaa atcgtgatcc cggaatttat taaactcggt attgaaccgc
    11881 aggacgctta cgactacgca gcgattggtt gtatcgaaac cgccgtcggt ggcaaatggg
    11941 gctatcgctg taccggcatg agctttatca acttcgcccg cgtgatgctg gcggcgctgg
    12001 aaggcgggcg tgatgccacc agcggcaaag tgttcctgcc acaagaaaaa gcgttgtcgg
    12061 caggcaactt taacaatttc gatgaagtaa tggacgcgtg ggatacgcaa atccgttatt
    12121 acacgcgtaa atcaatcgaa atcgaatatg tcgtcgacac catgctggaa gagaacgtgc
    12181 acgatattct ctgctcggcg ctggtggatg actgtattga gcgagcgaaa agtatcaagc
    12241 aaggcggcgc gaaatatgac tgggtttctg gcctgcaggt tggcattgcc aacctcggca
    12301 acagcctggc ggcagtgaag aaactggtat ttgaacaggg cgcgattggt cagcaacagc
    12361 tggctgccgc actggcggat gacttcgatg gcctgactca tgagcaactg cgtcagcggc
    12421 tgattaacgg tgcgccgaag tacggcaacg acgatgatac cgtcgatacg ctgctggctc
    12481 gcgcttatca gacctatatc gacgaactga aacagtacca taacccgcgc tacggtcgtg
    12541 gtccggttgg cggcaactat tacgcgggta cctcgtctat ctccgctaac gtaccgtttg
    12601 gcgcgcaaac tatggcaaca ccggacgggc gtaaagccca caccccgctg gcagaaggcg
    12661 caagcccggc ctccggtact gaccatcttg gccctactgc ggtcattggc tcagtgggta
    12721 aactgcctac ggcagcgatt ctcggcggcg tgttgctcaa ccagaaactg aatccggcaa
    12781 cgctggagaa cgaatctgac aagcagaaac tgatgatcct gctgcgtacc ttctttgaag
    12841 tgcataaagg ctggcatatt cagtacaaca tcgtttcccg cgaaacgctg ctggaggcga
    12901 aaaaacatcc ggatcagtac cgcgatctgg tagtgcgtgt cgcgggctat tccgccttct
    12961 tcaccgcgct ctctccagac gcacaggacg atatcatcgc ccgtactgaa catatgctgt
    13021 aatctcctcg gcccggcggc gtcgtcgccg ggctaaatca ttcacatcat caatttcatc
    13081 cttactttca ttcgaaatat aatttgtgct ctgcgtcaca ttgttattac actgttacgg
    13141 tttcattttc gagccaggag cacacctatg agcgtaaaag ttatcgtcac agacatggac
    13201 ggtacttttc ttaacgacgc caaaacgtac aaccaaccac gttttatggc gcaatatcag
    13261 gaactgaaaa agcgcggcat taagttcgtt gtcgccagcg gtaatcagta ttaccagctt
    13321 atttcgttct ttcctgagct aaaggatgag atctcttttg tcgcggaaaa cggcgcactg
    13381 gtttacgaac atggcaagca actgttccac ggcgaactga cccgacatga atcgcggatt
    13441 gttattggcg agttgctaaa agataagcaa ctcaattttg tcgcctgcgg tctgcaaagt
    13501 gcatatgtca gcgaaaatgc ccccgaagca tttgtcgcac tgatggcaaa acactaccat
    13561 cgcctgaaag ctgtaaaaga ttatcaggag attgacgacg tactgttcaa gttttcgctc
    13621 aacctgccag atgaacaaat cccgttagtg atcgacaaac tgcacatagc gctcgatggc
    13681 attatgaaac ccgtcaccag tggttttggc tttatcgacc tgattattcc cggtctacat
    13741 aaagcaaacg gtatttcgcg gttactgaaa cgctgggatc tgtcaccgca aaatgtggta
    13801 gcgattggcg acagcggtaa cgatgcggag atgctgaaaa tggcgcgtta ttcctttgcg
    13861 atgggcaatg ctgcggaaaa cattaaacaa atcgcccgtt acgctaccga tgataataat
    13921 catgaaggcg cgctgaatgt gattcaggcg gtgctggata acacatcccc ttttaacagc
    13981 tgacctctct accggagccg tgcgctccgg ttttcccttc tttttacatc ctgtacatca
    14041 catcaaatca ttaaacttgc attaacttat ttataactta tattgtcgca tgcaaatcag
    14101 aatggtttcg aatgaaagtt aatgagaggt ggttatggct tctactttta ccagcgacac
    14161 attgcctgcc gatcacaaag cagctatccg tcagatgaag cacgcgctgc gggcgcagct
    14221 tggcgacgtc cagcagatct ttaatcagct aagcgatgac attgccacgc gagtggctga
    14281 aatcaacgca ctcaaagcac agggcgatgc cgtctggccg gtgctgtctt atgccgatat
    14341 caaagcaggt catgttactg cagagcagcg cgaacagatt aaacgtcgcg gttgtgcggt
    14401 gataaaaggc catttccccc gcgaacaagc gctaggctgg gatcagtcga tgctggacta
    14461 tctggaccgc aaccgctttg acgaggtcta caaaggcccc ggcgataatt tcttcgggac
    14521 gctcagcgct tcacgtcccg agatttaccc catctactgg tcgcaggcgc aaatgcaggc
    14581 ccgccagagt gaagaaatgg cgaatgcgca gtcgtttctc aatcgtctgt ggacatttga
    14641 aagtgatgga aagcaatggt ttaacccgga tgtgagcgtc atctaccctg accgtatccg
    14701 ccgccgtccg cccggaacga cctccaaagg tcttggagcg cataccgact ccggggcgct
    14761 ggaacgctgg ctgcttccag cgtatcagcg cgttttcgcc aacgtcttta atggcaatct
    14821 ggcgcaatat gatccctggc atgcggcaca tcgtacggaa gttgaagagt acacggtgga
    14881 caacaccacc aaatgttccg tgtttcggac attccaaggc tggacagcgc tctctaatat
    14941 gctgcctggt caggggctgc tgcacgttgt gcccattcct gaagccatgg cgtacgtact
    15001 gttacgtccg ctgcttgatg atgtgccgga ggatgaactg tgcggcgtag cgcccggaag
    15061 agtgttgccg gtatcagagc aatggcatcc actgttaatt gaggcgttaa ccagcattcc
    15121 aaaactcgag gccggagact ccgtctggtg gcactgcgac gtcatccatt ccgttgcccc
    15181 cgttgaaaat caacagggtt ggggcaacgt gatgtacatt cctgcggcac caatgtgcga
    15241 gaaaaatctt gcctacgcgc acaaggtgaa ggccgcactg gaaaaaggcg catcgccggg
    15301 cgacttcccg cgcgaggact atgaaacaaa ctgggaagga cgctttacgc ttgccgacct
    15361 caacattcac ggtaagcgag cgttgggaat cgacagctga gtgtcgtaca gcccttcccg
    15421 ttccacggcg gtgttccctt ttcctgggcg aacccgccgc actgactccc gcaccgccaa
    15481 tcgtccgtgc agcagcagcg agcccaccgg agcatccggg cgaatcagcc gctgttgcaa
    15541 catttgcacc gcttcattgc ctagctcatc gcgtggcaca tgcacagccg tcaggggaac
    15601 atcctcgatt gccgcaaggt taaagccgtc gatactcatc accgagacat cctgtggtac
    15661 gcgcagacca cgacgatgta gcgcatttac cgttcccgcc gccataaaat ccccgccgac
    15721 cagaaacgcc gtcggcaacg tcttgccatt ggcggcatcc agccacgctc cgaccttttc
    15781 ttccgcttct ttagcgctaa aactgctgat attcagcaaa tgacggtcat ggtgaaaggc
    15841 cagattatgc tgacgccatg attctttaat ccctgcaaac cgctgctcca tagtgtagcg
    15901 gcgcaggcac atcacattca tcacctcccg gtgacccatt tcaaacagat agcgggcggc
    15961 gaacgcgcca attaaccgat gatccggcgc aatcgccggg aggcgcattc gttcatcacg
    16021 gcagttgatc agcacacaag gcttagcgag atcggcggcc agatcatgga tgtgcggatc
    16081 gtcaatcccc agcaggatcg ctgcctgcgt atctgcttca ttcatccgcg agagaaagag
    16141 cgcgggatcg ctgtcaaact cctccagcgc gcagtaacgc agccgcactt catgcggcga
    16201 taacgctttg ctgatgctct ggatcacacg gtaataaaag atatcagatc gttggtcaaa
    16261 agcgcgttgc ggcgcgaaaa taatcaggtt gttcagcaac atacgacctg ctgccatgcc
    16321 atccatcacg cccaactcac gcgcgcaggc cagcacctgt tcgcgagcgc gtgcactggt
    16381 attcgctttg ccagccagca cccgcgaaac cgtactcggc gatagcccgg tacgtgcggc
    16441 gatatcggcg attttcagct tcttgtccat tttgtgatct gcctctaatt cggaaatgac
    16501 agaattttca tgagcaaaaa agcccattct gtctggcatt aaatccattt cagtaatgta
    16561 tatcagtcac tcatggaaaa acgtgcacaa aacccgcttt ctttcgcttt tagggtctct
    16621 tactgtgaat tggtagaaca acttccatac aaaaataatg gaaaaacgaa caataaagac
    16681 tttatttatc aaaaagttaa aagtggtgta acaactccat tctgacgttg ccgcacaaac
    16741 cctataccat tctgtggagt gcatatgagt cagggtatca ataacgatat gactgccagt
    16801 aaaccgcgcc atatcgtcaa aaatctgcgt tggtggatac tggtcctttt tttgctcggt
    16861 gttactgtca actacatcac gcgtaactcg ctgggtattc tcgcccccga actgaaaagc
    16921 agcctaggga ttaccaccga acaatattcc tggattgtag gtgctttcca gctcgcctat
    16981 accctattcc aaccgttgtg cggatggctg attgacgtca ttggcctcaa actgggcttt
    17041 atgatctgcg cgggactgtg ggcactggcc tgtctgatgc atgcgggggc gggaaactgg
    17101 ggacatctgg cgctgttgcg cttctttatg ggcggtgcgg aagccgcggc aaccccagca
    17161 aacgccaaaa ccatcggtga atggtttcca aaatcagaac gcccgatcgc cgcaggctgg
    17221 gcaggcgtcg gtttttccat cggtgccatg ctggccccac cgattatcta ttttgcccac
    17281 gcttcattcg gctggcaggg tgcatttatg ttcactggca tcctggcgat gctgtgggtg
    17341 gtgctgtggt gggcgttcta tcacagcccg gataagcatc ctaacctcag caaagccgag
    17401 ctggacttta ttcgccagga taacgaagcg cccccggtca agttaccctt tctgaccgca
    17461 ctgaaaaccg tgggcaaaaa taaacgcttt tacggtatcg ctatcccggc ctttatggca
    17521 gaaccggcgt gggcggtgat gagtttctgg gtgcctctat acctgtccaa aacctatggc
    17581 atggacctga agcagatcgc attattcgcc tggttgccgt tcctcgccgc tgatcttggc
    17641 agtattgcca gcggctatct gaccaaactt tacacccgcc tgtttgggct gagtcgtgtg
    17701 aactccgttg tcgcatcatc ggtcagtggc gcattcttga tggtttcgct ggcggtgatg
    17761 gcctttacca ataacccgta tatcgccatc gtccttatct ccatcggcgg cttcggtcac
    17821 cagatcatct cctgcatgct gagcgcactg gtggtggaat ccttcgacaa aggtcagatg
    17881 gccactgtta acggcatgcg cggttctgcc gcgtggatcg caagtttcct gttctccctg
    17941 attatcgggg ttaccgctga caaaatcggc tttaaccccc tgtttatcgc catgggcttc
    18001 tttgacctga ttggcgcttt cttcctggtc acctttattg ctgaacgtcg cgcaaaacgc
    18061 gcctgagagt ggatgtaaac atgaaaacgc tgaaaaactg gaccctgcac aaacagtctg
    18121 cccaccacgt tgaactgctg gtcgatggcc agcatatcct gtgcctgtac gtgctggaag
    18181 aaaatctgtt ccgcgtgctg ttaaaacgca aaggcgagct ggcgctagat cgcacctgga
    18241 gcattgctcc ggcgcaggat gtgccatggg aagggcgcag ccgcgaagat ctcagcggct
    18301 ttagcctgcc cgcgtggaca gtgatccagc acgacgacac cgtggttatc gccaccgagt
    18361 cactgcgtgc aaccgttcac cagccgctgt ggctggagtg gcactatcgt aacaacgaag
    18421 gcgaatggct gccgttggtg aatgaccgcc cgaccagcgc ttatctgctg aatgcccacg
    18481 gcgatggcgt ggcacactat cagagccgtc gtaaagatga acgcttttat ggcctcggag
    18541 ataaagccgg cgatctgcaa cgcaacggta aacgttacga gatgcgtaac ctcgacgcga
    18601 tgggctacaa cgccgttagt actgacccgc tgtacaaaca cattccattc accatcaccc
    18661 gccgcgatga cattagcttc ggcctgtttt atgacaacct cagcagctgc tggctggatc
    18721 tgggcaacga gatcgacaac taccacaccg cctatcgccg ctggcaggcg gaagcgggtg
    18781 atatcgacta ctacctgttt accggcgaac gcgtacttga tatcaccaaa gccttcgtac
    18841 gcctgaccgg aaaaaccctg ttcggaccga aatggagcct gggctacagc ggttcaacta
    18901 tgcactacac cgatgcgccg gacgcacaaa atcagttgat gaactttatc cgcctgtgtg
    18961 aagaacatgc aattccctgc gattcgttcc agctctcttc cggttatacc tcaattaacg
    19021 gcaaacgcta tgtgttcaac tggaactacg acaaagtgcc gcagccgaaa gtgatgagcc
    19081 aggcatttca caatgctggt ttgcacctgg ccgccaatat caagccgtgc ctgttgcagg
    19141 atcacccacg ctataacgaa gtggcagaaa gaggactgtt catccgtgat tcagaaacag
    19201 atgaaccaga acgttccagc ttctgggatg acgaaggttc taacctcgag tttactaatc
    19261 cgcagaccat ccagtggtgg caggaaggcg tgaccacaca gcttctggag atgggtatcg
    19321 actccacctg gaacgacaac aacgaattcg aagtatggga cggtgaagca cgctgtcacg
    19381 gctttggtca gacgatcgcg atcaaacata ttcgcccagt gatgccactg ctgatgatgc
    19441 gagcctcgat ggaagcccag cagcgtttcg ctccggaaaa acgtccgtac ttgatctccc
    19501 gctccggctg cgcgggaatg cagcgctacg tgcagacctg gagtggcgat aaccgcacta
    19561 gctgggacac tctgcgctac aacacccgca tgggtctggg gatgagcctc tccgggctgt
    19621 ataacctcgg tcacgacgtc ggcggtttct ccggtgataa gccggacccg gaactgtttg
    19681 tacgctgggt acaaaacggc gtgatgcatc cgcgctttac gattcactca tggaacgatg
    19741 accataccgt aaacgaaccg tggatgtatc cgggcgtcac tccggcgatc cgcagcgcta
    19801 ttgagttgcg ctatcgcctg ctgccctact tttataccct gatgtggcag gcatacgccg
    19861 atgatgaacc aatgctacgt ccgactttcc ttgaccacga gcacgacgtt cagacttttg
    19921 aagagtgtga tgacttcctg cttggtcgcg atattttggt tgccagcgtc gtagagcaag
    19981 gcgagcgcca gcgccgtgta tggctgccag ataacgaaac tggctggtac gatttctaca
    20041 acggcgaatg gttcagtggc ggtcagtgga tcaccctcga cgcaccgctg gagaaactgc
    20101 cgctgatggt tcgtgccggg gctggtctac cgctcagcaa acgcatcacc tatgtgagcg
    20161 cagaacaaga cgatacccgc gaactgaaac tgttcccgct gaaaggtgtg ggcacaacgt
    20221 ccggcctgct gtttgaagac gatggcgaaa gctggggcta ccaaactggt aatgcgcttt
    20281 gggtggaatg ggaaatggtt tgcgatgggg cgaccatcaa cctgaaagtc aacgcgcgcg
    20341 gggattatcg tcctgcatgg agtgcactga aagtgtcatt accattggaa gaaaaacgca
    20401 cgctgctggt caatggtgtt gaagggagtg aatggatgcg ttaaatcatt accgggaccg
    20461 ggaggcgact gcaccttacc cggtccagcc atcgaatcct ttacctcagt atttcacgct
    20521 gcaggaaggc agcaaacctg taaaccccgg gagcttaaat aagtaagtga ccggggtgaa
    20581 caggtgaagc caatgcatct gccgcatgaa agacggcggg gaattaagcg tcgatccctt
    20641 tactacgcag gtaatcttcg taattaccgc taaagtcgat cgcgcgttcc ggggtgattt
    20701 ccagaatgcg ggtcgccagg gagcttacga actcacggtc gtgtgaaaca aagatcagcg
    20761 tgccctgata cagttccagc gccatgttca gtgactcgat ggattccata tccaggtggt
    20821 tggtcggttc gtccatgatc agaatgttcg gcttctgcat cattaactta ccaaacagca
    20881 tccgcccttt ttccccaccg gaaagcactt tagctggctt tttgatgtcg tcctggctga
    20941 acagcaaacg accgagaatg ctgcgtaccg cctgctcgtc atcgccttcc tgcttccact
    21001 gactcatcca ttcgaacacg gtcagatcat tttcaaactc atattcgtgg tcctgggcgt
    21061 agtaaccaat gcgcgcgttt tcagaccatt ttacggtgcc gctgtccggt tgcagatcgc
    21121 ccaccagcgt tttcagcagc gttgatttac cgacgccgtt ggtacccagt accgcaagtt
    21181 tttcaccgac ctccagcagc aggttgagat ttttaaacag tggaccgtta tcaaaccctt
    21241 tggtcagacc ttccacttcc agcgcgttac ggaacagttt cttatcctgt tcaaaacgga
    21301 tgaacgggtt ctgacggctg gaggctttca cctcttccag tttgatttta tcaatctggc
    21361 gcgcgcgcga agttgcctgg cgagatttcg aggcgttggc gctaaagcgg ctaacgaaag
    21421 attgcaactc agcaatctgc gctttcttct tggcgttatc ggccagcaga cgttcacgcg
    21481 cctgggtcgc cgccgtcatg tactcatcgt agttacccgg ataaacgcgc agctcgccgt
    21541 aatccagatc cgccatgtgg gtacagacca tgttaaggaa gtgacggtcg tgcgagatga
    21601 tgatcatggt gctgtcacgc tcgttcagca cctgttccag ccagcgaatg gtgtcgatgt
    21661 cgaggttgtt ggtcggttcg tcgagcagga gaatctccgg atcagcaaac agcgcctgcg
    21721 ccagaagcac acgcagtttc cagccaggag caacttcact catcgggccg tagtgctgtt
    21781 ccactggaat tcccacgcca agcaacagtt caccggcgcg agcttccgca gagtaaccgt
    21841 ccatttcgcc gtatttaact tccagatcgg ccactttata gccgtcttcc tcactcattt
    21901 ccggcaaagc atagatgcgg tcacgctcct gcttcacttc ccacaactct ttatgcccca
    21961 tgatcaccgt atccagcaca gtgaactctt caaaggcaaa ctgatcctga cgcagcttac
    22021 caatgcgctc gttgggatcg agggaaacgt tacccaaagt cggctctaag tcgccgccga
    22081 ggatcttcat aaaggtggat ttaccactac cgttcgcgcc aatcaggccg taacggttgc
    22141 cgccgccgaa tttgacggaa atgttttcaa acaacggctt actgccgaac tgcatggtga
    22201 cgttactgga aactaacaca ggcgtatcct gaaaagagat atgacaaacc gcgcattatg
    22261 ccataagtct gaaagaagat cctgctcctc gcttaaggtc tacactcaag ccaaaggcaa
    22321 aaaatgtgat ttcgtacaca tctgatttca ctgtgagctg gaatgaactt ataatgcgct
    22381 tccaatactc taatattctc aacccaatgg cctgccaggc acaaaatctc gcttaacatg
    22441 aatatgaaat tgaaaacatt attcgcagcg gccttcgctg ttgtcggctt ttgcagtacc
    22501 gcctctgcgg taacttatcc tctgccaacc gacgggagtc gcctggttgg tcagaatcag
    22561 gtgatcacca ttcctgaagg taacactcag ccgctggagt attttgccgc ggagtaccag
    22621 atggggcttt ccaatatgat ggaagcgaac ccgggtgtgg ataccttcct gccgaaaggc
    22681 ggtactgtac tgaacattcc gcagcagctg atcctgccgg ataccgttca tgaaggcatc
    22741 gtcattaaca gtgcagagat gcgtctgtat tactatccga aagggaccaa caccgttatc
    22801 gtgctgccga tcggcattgg tcagttaggc aaagatacgc ctatcaactg gaccaccaaa
    22861 gttgagcgta agaaagcagg cccgacctgg acgccgaccg ccaaaatgca cgcagagtac
    22921 cgcgctgcgg gcgaaccgct tccggctgtc gttccggcag gtccggataa cccgatgggg
    22981 ctgtatgcac tctacatcgg tcgcctgtac gctatccatg gcaccaacgc caacttcggt
    23041 atcggcctgc gtgtaagtca tggttgtgtg cgtctgcgta acgaagacat caaattcctg
    23101 ttcgagaaag taccggtcgg tacccgcgta cagtttattg atgagccggt aaaagcgacc
    23161 accgagccag acggcagccg ttatattgaa gtccataacc cgctgtctac caccgaagcc
    23221 cagtttgaag gtcaggaaat tgtgccaatt accctgacga agagcgtgca aacagtgacc
    23281 ggtcagccag atgttgacca ggttgttctt gatgaagcga ttaaaaaccg ctccgggatg
    23341 ccggttcgtc tgaattaatc ttcaaaactt aaagcaaaag gcggactcat aatccgcctt
    23401 ttttatttgc cagaccctag ttggccggga gtataactaa caaaacatat cccaccaacg
    23461 ccgcccacaa cagcatcggt attgaataga gatggaaacg ccaccagatg cggcgatcgt
    23521 tcgccatacg tagcgcaatc aaatttgcca gcgatccggg taataaccca aagccaccta
    23581 cgtttaccgc ccataccagt aataaagacg gcggcacata gttcagcaac aatatggtac
    23641 tcggcacgtt actgatcacc tgcgataaac cgattgccgt taaccataac ccgggttcag
    23701 atagatgact cacgttaccc aacacgcctt gcaacgctgg aagctgggtc agtaaatgga
    23761 cgtcgataaa catcgccata aacaccagca gcagcgtcca gtccacactg agcaccacgc
    23821 gacgtgccag cagcgcaaag cctgccgcca caatcaccag tccccacagc tcttgtttga
    23881 actccagcgc cgtcagaaag acgatataca gccccagaca actccacacc agccgcggtt
    23941 tccactccgg tgtttgcacc cccgtatggt attgcagcgc ctttccaggg aaacaacacc
    24001 agcacaggag caggagcgtc agcatcattg cgccagccag cggtgccatt tgggcaataa
    24061 atccggcaaa cgaaagacca gaacgtcccc agataagaat attttgcggg ttgccaattg
    24121 gcgtcagtag cgaaccagcg ttgactgcca gcgcctcaaa aataatcagc cgattaaccg
    24181 ggatctcaca cagtcttttt agcgtgatag tcagcggaac aacaataaac agcgcgacat
    24241 cgttggtcag aaaggtagaa agcagcgccg ccgccagcac cataaacatc gccagccgac
    24301 gctccgtagc aaagcggcgc accattttgc gccccagcac atcaaaataa ccgcttaact
    24361 ccacaccttt ggtcagcagc atcaggccgc ttaaggtgat gatggtgtgc cagtcgatag
    24421 cagcaggcca ggatttcggt gcaaagggca cgaaaaagct taatccgata ccaacaagaa
    24481 ttaataactg aaaaaaacga tcgccttgca gcgtgcgtaa aaaaggcagg ctcatttggc
    24541 accgtgtttc tgggtaaaca aacgaaaagc gtcgagcgtc tcttcactaa catggtgctc
    24601 catgccttcc gcgtcgcgac gggcgatttc cggactgacg cccaacacca acaagaaatt
    24661 ttcgactatc tgatgtcgct cgcggctttc ctgcgccagc ttctctcctt ctgccgttaa
    24721 aaacacgcca cgccagggga tcatttcaat cagccccatg gttgccagcc gcttaagcat
    24781 tttagccacc gtcggttgcg aaactcccag acgagcagcc atgtccacct gacgagcttc
    24841 ccccacttcc ctgatcaagt cagaaatcag ctcaacgtaa tcatcaataa gctcgcgccg
    24901 atgcgcctct ctgacctggc ggaacccttc aacgtgctct tccacgttca ctaactgcgt
    24961 cacttttttt gctgttggcg tacctgcgcg acgactcatt gtgcttcctc attatggtga
    25021 cgcatacata gcgttctcat ttcgtgcgca cattgtaaac cagagttgcg aaggtacaaa
    25081 aaattaacgt tttagcaata gctacataat atagcctgtg ctatatctgt atgtaatgca
    25141 atcatccctc aaggatcgac gggattagca agtcaggagg tcttatgaat gagttcaaga
    25201 agtgtatgcg cgtgtttagt cattctccct ttaaagtacg gttaatgctg ctctctatgt
    25261 tgtgcgatat ggtcaacaac aaaccgcagc aagataaacc ttccgataaa tagcggcgtc
    25321 gcggtacgcc gcttcactcc tgcttttatg caggcataac gcgttttggt ctgaaaaacc
    25381 ccactttttg tcggatttgc aatccccttc gcaaaagatt tgttcgtcag tagttgacct
    25441 gaacggcggc tcgctctatc ttcttgcagc cctacgtata ttgcggctcg cggatgcgga
    25501 cccctttccc ctcttcacgc actcttgcag gtattgaccc ttgacgccag ggtaagcaca
    25561 tggcgtttgt tacgatagtg gcatatgaat ttaaccctca aagaatcgct tgttacccgt
    25621 agccgggtat ttagcccgtg gactgcgttc tactttttac agtcgctatt aattaacctc
    25681 ggcttaggtt accccttcag tttgctctac accgctgcgt ttacggctat tttgcttttg
    25741 ctatggcgaa cattgcctcg cgtacaaaaa gttctggtcg gtgtcagttc gctggtggcg
    25801 gcttgttatt tcccttttgc tcaggcctac ggcgcgccta atttcaatac attgctggca
    25861 ttgcactcca ccaatatgga agagtcgacc gaaatcctga cgatttttcc gtggtacagc
    25921 tacctggtcg gcttatttat ttttgcgctc ggcgtaatag caatcaggcg aaaaaaagag
    25981 aatgaaaaag cgcgctggaa taccttcgac agcctgtgtc tggtattcag tgtggcgaca
    26041 ttttttgttg ctcccgtgca aaacctggcc tggggtggcg tatttaaact gaaagatact
    26101 ggctatccgg tatttcgttt tgctaaggat gtcatcgtca ataataacga ggtgattgaa
    26161 gagcaagaac ggatggcaaa actttccgga atgaaagata cctggacggt cactgccgtt
    26221 aagccgaagt atcagaccta tgtggtggtg atcggcgaaa gcgcgcgtcg cgatgccctc
    26281 ggtgcctttg gcggtcactg ggacaatacc ccgtttgcca gcagcgttaa cggtttgata
    26341 tttgctgact acattgccgc cagtggctcc acgcagaaat cacttggctt aacgctcaat
    26401 cgcgtagtcg atggcaaacc acagtttcag gataactttg tcaccctggc aaatcgcgcg
    26461 ggcttccaga cctggtggtt ttccaaccag ggccaaatcg gcgaatacga tactgctatc
    26521 gccagcatcg ccaaacgtgc agatgaagtg tacttcctga aagaaggtaa tttcgaagca
    26581 gataaaaaca cgaaagacga agcgttactg gatatgaccg ctcaagtgct ggcgcaagag
    26641 cactcgcaac cgcagctgat tgttctgcat ctgatgggct cgcatccgca ggcctgcgac
    26701 aggacacaag gcaaatacga aacctttgtg caatcgaaag aaacgtcgtg ctatctctat
    26761 accatgacgc aaacggacga tttactgcgc aagctgtacg atcagttacg caacagcggc
    26821 agcagcttct ctctggttta cttttctgac cacggtctgg cctttaaaga gcgcggcaaa
    26881 gacgtgcaat accttgccca tgatgataaa tatcagcaaa atttccaggt gccttttatg
    26941 gtcatttcca gcgacgataa agcgcatcgg gtgattaaag cccgccgctc agccaatgac
    27001 ttcttaggct ttttctcgca gtggacggga attaaagcga aggaaataaa tatcaaatac
    27061 ccgtttatat ctgagaagaa agccgggccg atatacatca ccaacttcca gttacagaag
    27121 gtggattaca accatctcgg aaccgatatt ttcgacccga aaccttaaaa caaaaaatcc
    27181 gccccgagag gcggattttt tatatcacca aagtgattag aagcggtaac caacaccggc
    27241 aatccaggtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc
    27301 aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt
    27361 gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca cccccactac
    27421 accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta
    27481 ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga
    27541 accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc
    27601 cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt
    27661 cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc
    27721 aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca
    27781 aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga
    27841 acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag
    27901 tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt
    27961 ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac
    28021 cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga
    28081 aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt
    28141 tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtggg
    28201 cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt
    28261 taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt
    28321 ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat
    28381 tgcggtggcg ctggagttca ccggaccact ggcggtggcg ctgttctctt ctcgtcgccc
    28441 ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg
    28501 gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg
    28561 ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt
    28621 ggcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg
    28681 tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac
    28741 cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg
    28801 tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga
    28861 aacactgaca cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc
    28921 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat
    28981 ttctgcatgg ttatgcataa ccatgcagaa tttctcgcta cttttcctct acaccgtctt
    29041 tatatatcga attatgcaaa agcatattta ttccgaaaat tcctggcgag cagataaata
    29101 agaattgttc ttatcaatat atctaactca ttgaatcttt attagttttg tttttcacgc
    29161 ttgttaccac tattagtgtg ataggaacag ccagaatagc ggaacacata gccggtgcta
    29221 tacttagtct cgttaattac tgggacataa catcaagagg atatgaaatt atgagtaccg
    29281 ctaaattagt taaatcaaaa gcgaccaatc tgctttatac ccgcaacgat gtctccgaca
    29341 gcgagaaaaa agcaacagta gagttgctga atcgccaggt tatccagttt attgatcttt
    29401 ctttgattac caaacaagcg cactggaaca tgcgcggcgc taacttcatt gccgtacatg
    29461 aaatgctgga tggcttccgc accgcactga tcgatcatct ggataccatg gcagaacgtg
    29521 cagtgcagct gggcggtgta gctctgggga ccactcaagt tatcaacagc aaaaccccgc
    29581 tgaaaagtta cccgctggac atccacaacg ttcaggatca cctgaaagaa ctggctgacc
    29641 gttacgcaat cgtcgctaat gacgtacgca aagcgattgg cgaagcgaaa gatgacgaca
    29701 ccgcagatat cctgaccgcc gcgtctcgcg acctggataa attcctgtgg tttatcgagt
    29761 ctaacatcga ataaatccat cgctgatggt gcagaacttt agtacccgat aaaagcggct
    29821 tcctgacagg aggccgtttt gttttgcagc ccacctcaac gcacttattt agtgcatcca
    29881 tctgctatct ccagctgatt aagtaaattt tttgtatcca catcatcaca caatcgttac
    29941 ataaagattg ttttttcatc aggttttacg ctaaataatc actgtgttga gtgcacaatt
    30001 ttagcgcacc agattggtgc cccagaatgg tgcatcttca gggtattgcc ctataaatcg
    30061 tgcatcacgt ttttgccgca tctcgaaaaa tcaaggagtt gcaaaactgg cacgattttt
    30121 tcatatatgt gaatgtcacg caggggatcg tcccgtggat agaaaaaagg aaatgctatg
    30181 aagtctgtat taaaagtttc actggctgca ctgaccctgg cttttgcggt ttcttctcat
    30241 gccgcggata aaaaattagt tgtcgcgacg gataccgcct tcgttccgtt tgaatttaaa
    30301 cagggcgata aatatgtggg ctttgacgtt gatctgtggg ctgccatcgc taaagagctg
    30361 aagctggatt acgaactgaa gccgatggat ttcagtggga tcattccggc actgcaaacc
    30421 aaaaacgtcg atctggcgct ggcgggcatt accatcaccg acgagcgtaa aaaagcgatc
    30481 gatttctctg acggctacta caaaagcggc ctgttagtga tggtgaaagc taacaataac
    30541 gatgtgaaaa gcgtgaaaga tctcgacggg aaagtggttg ctgttaagag cggtactggc
    30601 tccgttgatt acgcgaaagc aaacatcaaa actaaagatc tgcgtcagtt cccgaacatc
    30661 gataacgcct atatggaact gggcaccaac cgcgcagacg ccgttctgca cgatacgcca
    30721 aacattctgt acttcatcaa aaccgccggt aacggtcagt tcaaagcggt aggtgactct
    30781 ctggaagcgc agcaatacgg tattgcgttc ccgaaaggta gcgacgagct gcgtgacaaa
    30841 gtcaacggcg cgttgaaaac cctgcgcgag aacggaactt acaacgaaat ctacaaaaaa
    30901 tggttcggta ctgaaccgaa ataataacgc tacacctgta aagcgcactg gcagttccct
    30961 ctcccctctg gggagaggat tagggtgagg ggcgcaaacc cgctccgggg ccattaatta
    31021 ccctgaattt gattatttac accacggtaa caggaacaac atatgcagtt tgactggagt
    31081 gccatctggc ctgccattcc gcttctgatt gaaggtgcca aaatgaccct gtggatttcg
    31141 gtcctcggtc tggctggcgg tctggtaatc ggattgctgg caggttttgc acgcaccttc
    31201 ggtggttgga tagccaacca cgtcgcgctg gtctttattg aggtgatccg cggcacacct
    31261 atcgtcgtcc aggtgatgtt tatttatttc gccctgccga tggcgtttaa cgacttacgc
    31321 atcgacccat ttactgcggc ggtggtcacc atcatgatca actccggcgc gtacattgcg
    31381 gaaatcacgc gtggtgcggt gctgtctatt cacaaaggtt ttcgtgaagc aggactggcg
    31441 ctcggtcttt cacgttggga aaccattcgc tacgtcattt taccgctggc actgcgtcgt
    31501 atgctgccgc cgctgggtaa ccagtggatc atcagcatta aagacacctc gctgtttatt
    31561 gtgatcggcg tggcggaact gacccgtcag gggcaagaaa ttattgccgg taacttccgc
    31621 gcccttgaga tctggagcgc cgtggcggtg ttctatctga ttattaccct ggtgctaagc
    31681 tttattttgc gtcgtctgga aagaaggatg aaaatcctgt gattgaattt aaaaacgtct
    31741 ccaagcactt tggcccaacc caggtgcttc acaatatcga tttgaacatt gcccagggcg
    31801 aagtcgtggt gattatcggg ccgtccggtt ccggtaaatc gaccctgctg cgctgcatca
    31861 acaaactgga agaaatcacc tccggcgatc tgattgtcga tggcctgaag gttaacgatc
    31921 cgaaagttga cgagcgcctg attcgccagg aagcaggtat ggtgttccag cagttttacc
    31981 tcttcccgca tctgacggcg ctggaaaacg tcatgtttgg tccgctacgc gtgcgtggcg
    32041 cgaacaaaga agaggcggaa aaactggcgc gtgagctgct ggcgaaagtc ggtctggcag
    32101 aacgtgcaca tcactaccct tccgaacttt ctggtggtca acagcagcgt gtggcgattg
    32161 cccgcgcgct ggcggtgaag ccgaaaatga tgctgtttga tgaaccgact tccgctcttg
    32221 acccggaact gcgccatgaa gtgctgaagg ttatgcagga tctggctgaa gaagggatga
    32281 cgatggtgat cgtgacccac gaaatcggtt ttgccgagaa agtagcttcg cggctgatct
    32341 ttatcgacaa aggccggatt gcggaagatg gcgatccgca ggtattgatc aagaacccgc
    32401 cgagccagcg cttgcaggaa tttttgcagc acgtctctta acttctctgc ctccctttca
    32461 cccgaaaggg aggcacacca gattcctctc atttaaaatc gcccctcccc cagcatctat
    32521 acttatcttt ttgctctgtt ttctcactgg aggagtcatg cggtggatcc tgttcatcct
    32581 cttctgcctg ctgggcgcac ctgcccacgc ggtatccata cccggcgtta caaccacaac
    32641 gacaacggac tcaacgactg aaccggcccc ggaaccggat atcgaacaaa aaaaagcggc
    32701 ctatggcgca ctggcggatg tgctggataa cgacacctcg cgtaaagaat tgatcgacca
    32761 gttgcgtact gtcgccgcga cgccccctgc ggaaccggtc ccgaagatcg tgccgccgac
    32821 gctggttgaa gagcaaaccg tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga
    32881 agccctttcc gcccgcttcg ggcaacttta tcgcaatatc accggctccc cgcataagcc
    32941 atttaatcca caaaccttca gcaatgcgct gagccatttt tcaatgttag cggtattagt
    33001 gtttggtttt tactggctga ttcgcctgtg cgcactgccg ctgtatcgca aaatgggtca
    33061 gtgggcgagg caaaaaaatc gtgagcgcag caactggttg cagcttccgg cgatgattat
    33121 cggggcgttt attatcgacc tgctgttact ggccctgaca ttatttgtcg gccaggtatt
    33181 aagcgacaac ctgaatgcag gcagtcgcac catcgctttc caacaaagtt tgtttctcaa
    33241 cgcctttgcc ctgattgaat ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt
    33301 ggcggagctg cgcccgttca cgattcagga cgagagcgcc cgttactgga gtcgtcgcct
    33361 gagctggtta agcagtttga taggttatgg cctgattgtg gccgtgccga ttatctctaa
    33421 tcaggtgaat gtacagatag gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt
    33481 ctgggcgttg tacctgatct ttcgtaataa aaaagagatt acccagcatt tgctcaactt
    33541 tgcggagcat tcgctggcct ttttcagcct gtttatccgc gcctttgcgc tggtgtggca
    33601 ctggctggca agcgcctatt ttatcgtgct gtttttcttt tcgttgttcg atccgggcaa
    33661 cagcctgaaa tttatgatgg gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc
    33721 gtttgtttcc ggtatgtttt cccgctggct ggcaaaaacc atcactctct cgccacatac
    33781 tcagcgtaac tatccagagt tgcaaaaacg gctgaatggc tggctttcgg cggcgctgaa
    33841 aacggcgcgt attctgaccg tctgcgtggc ggtaatgttg ctgttgagcg catggggatt
    33901 gttcgatttc tggaactggc tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat
    33961 ccgtatcgca cttattcttt ttttctcggc ggttggctgg acggtgctcg ccagtttgat
    34021 cgaaaaccgg ctggcttcgg atattcatgg ccgcccgcta cccagcgccc gtacgcgtac
    34081 cctgctgacg ctgttccgta acgcgctggc ggtgattatc agtaccatca ccatcatgat
    34141 tgtgttgtcg gaaatcggcg tcaatatcgc gccattgctg gcaggtgccg gggcattagg
    34201 tctggctatc tcgtttggtt cgcaaacgct ggtgaaagat atcattaccg gggtgtttat
    34261 tcagtttgaa aacggcatga acactggaga tttggtgact atcgggccgt tgaccggcac
    34321 agtggaacgg atgtcgattc gctccgtggg cgtgcgccag gataccgggg cgtatcacat
    34381 cattccgtgg tcttcgataa ccacctttgc taacttcgtc cgcggtattg gttcggtagt
    34441 ggcaaactac gatgttgatc gccatgaaga tgctgataaa gccaatcagg cactgaaaga
    34501 tgcggtagcg gaattaatgg aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa
    34561 ttttgccggg attgtcggct taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac
    34621 gctgccactc aaacagtgga cggtacgctt tgccctcgac agccaggtga aaaaacattt
    34681 cgacctggcg ggcgttcgcg cgccagtgca gacttatcag gtgctgccta ctccgggcgc
    34741 gaccccggct gaaccgttac cgccggggga gccaacgctt taacgctggc gattgacaaa
    34801 acggcggcgc tgctcgtcgt tcataaaggt ccaggcaata aagcgactct gcttttgccc
    34861 ctgggccatc tcttttttta ccaccttcac cgcgcccacg tcggtcagag cacgatacaa
    34921 cggcggtaag ttttcaccac gagataccag cgaggtaaac cacatcacct gcttcgcgaa
    34981 gcctttgctc tcttcaatca tctttttgat aaaggcgact tcaccacctt cacaccacaa
    35041 ctcctgttgc tgaccaccaa agttcagtgc atcgtctttg ttcagcccca ggttacggcg
    35101 tttacgctca ctacctgccc gcgctgcagc ggcggaatcg tggaatggcg ggttacacaa
    35161 ggtcgcgtcg tattgctcgt ttttatggat gatgccgtta aaaattgccc cactctcttt
    35221 ttgccgacgc agacgaatgg cgcggttaag ccccggatta gcactgataa tcgcctgcgc
    35281 actgcttaac gcctggctgc tggtttcact accggtaaaa cgccagccat attcatgtac
    35341 gccaatcagc ggataaatac agttcgcacc aacgccgata tccagaatgc tggcattcgc
    35401 tggaattgtt ccgcttgctt cagccagtaa atcggcaagg tgatgaatat aatccgcccg
    35461 gcccggtact ggtgggcaga gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa
    35521 atgggccagc aacgccttat tgagcgcctt caccgccagc ggattggcaa agtctacgct
    35581 ttgctccccg gcgggtgtaa gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag
    35641 cgtggcgaga tcgtagcggc tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc
    35701 ggacatagca ttctccttta agcagcggcg taagataccc gcataacgcg acagggtaaa
    35761 tatctggctg cgtttatcat tccccaaaac cttaacatgg ctatggttta tggcaattca
    35821 tcataaaaat cagcattatg catgtcatta atttgtctta ttttgtaaaa atatatcgtt
    35881 caaaaaataa gcacatctct gtcaattttt tgcgtcattt gcatcgcgca ggcgacctac
    35941 acttatcagg cactacccgt tgtatttatg aggatgtcat tatgaaaaag tgcctcacac
    36001 tactgattgc caccgtcctg agcggaatct ctctcacggc ttatgccgcg caaccgatga
    36061 gtaacctgga cagcggtcag ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa
    36121 acctaagcga tctggaggat aaactggcag aaaaagcgcg cgaacaaggc gcgaaaggtt
    36181 atgtcattaa ttccgcaggc ggaaatgacc agatgttcgg tactgcaacc atctacaaat
    36241 aaccccacca acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa
    36301 aaaacatcaa atatgaactc aatgtaaata aatgtatttc tttttcgcgc aatgggtgat
    36361 agaaaatcgc gccaagtgat aatgcttatc aaaattatta tcacttgcat gaacactatc
    36421 acgggattaa cagtggcatc gcatccgcag agatgctttc tcgtggcagt gaaaatttca
    36481 acatataaga aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct gccagacaat
    36541 ttcattcgct cacgttcttt gccggtcttt gtattggcat cacgcctgtg gctcaggcac
    36601 tcgccgccga agggcaaact aacgcggatg acacgctggt tgtcgaagca tcaacgcctt
    36661 cgctttatgc gccacaacaa tctgccgatc cgaaattctc gcgtccggta gcggatacta
    36721 cccgcacgat gacggtgatt tctgaacaag tgattaaaga tcagggcgca accaacctta
    36781 ccgacgcgct caaaaacgcc cccggcg
//
LOCUS       DAJTYZ010000028        36710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000028 DAJTYZ010000000
VERSION     DAJTYZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.028"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1092
                     /gene="dacC"
                     /locus_tag="OO596_001342"
                     /pseudo
     CDS             1..1092
                     /gene="dacC"
                     /locus_tag="OO596_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="OO596_001343"
     CDS             complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="OO596_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6717244.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="OO596_001344"
     CDS             complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="OO596_001344"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6717245.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2836..4063
                     /gene="mdfA"
                     /locus_tag="OO596_001345"
                     /pseudo
     CDS             2836..4063
                     /gene="mdfA"
                     /locus_tag="OO596_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4104..4388)
                     /locus_tag="OO596_001346"
     CDS             complement(4104..4388)
                     /locus_tag="OO596_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717246.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="OO596_001347"
     CDS             complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="OO596_001347"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6717247.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5289..6497)
                     /locus_tag="OO596_001348"
     CDS             complement(5289..6497)
                     /locus_tag="OO596_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6717248.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6581..7117
                     /gene="rcdA"
                     /locus_tag="OO596_001349"
     CDS             6581..7117
                     /gene="rcdA"
                     /locus_tag="OO596_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6717249.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7292..8977)
                     /locus_tag="OO596_001350"
     CDS             complement(7292..8977)
                     /locus_tag="OO596_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6717250.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9247..9624
                     /locus_tag="OO596_001351"
     CDS             9247..9624
                     /locus_tag="OO596_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6717251.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="OO596_001352"
     CDS             complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="OO596_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6717252.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10071..10358
                     /locus_tag="OO596_001353"
     CDS             10071..10358
                     /locus_tag="OO596_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6717253.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10342..11064
                     /gene="nfsA"
                     /locus_tag="OO596_001354"
     CDS             10342..11064
                     /gene="nfsA"
                     /locus_tag="OO596_001354"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6717254.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            11125..12027
                     /gene="rimK"
                     /locus_tag="OO596_001355"
     CDS             11125..12027
                     /gene="rimK"
                     /locus_tag="OO596_001355"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6717255.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            12115..12591
                     /locus_tag="OO596_001356"
     CDS             12115..12591
                     /locus_tag="OO596_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6717256.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12942..14054
                     /gene="potF"
                     /locus_tag="OO596_001357"
     CDS             12942..14054
                     /gene="potF"
                     /locus_tag="OO596_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6717257.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            14149..15282
                     /gene="potG"
                     /locus_tag="OO596_001358"
     CDS             14149..15282
                     /gene="potG"
                     /locus_tag="OO596_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6717258.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            15292..16245
                     /gene="potH"
                     /locus_tag="OO596_001359"
     CDS             15292..16245
                     /gene="potH"
                     /locus_tag="OO596_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6717259.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            16242..17087
                     /gene="potI"
                     /locus_tag="OO596_001360"
     CDS             16242..17087
                     /gene="potI"
                     /locus_tag="OO596_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6717260.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMVRAEKQRIRDIQ
                     RARHG"
     gene            17147..17635
                     /locus_tag="OO596_001361"
     CDS             17147..17635
                     /locus_tag="OO596_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6717261.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            17676..18803
                     /gene="rlmC"
                     /locus_tag="OO596_001362"
     CDS             17676..18803
                     /gene="rlmC"
                     /locus_tag="OO596_001362"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6717262.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(18978..19709)
                     /gene="artJ"
                     /locus_tag="OO596_001363"
     CDS             complement(18978..19709)
                     /gene="artJ"
                     /locus_tag="OO596_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6717263.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(20001..20669)
                     /gene="artM"
                     /locus_tag="OO596_001364"
     CDS             complement(20001..20669)
                     /gene="artM"
                     /locus_tag="OO596_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6717264.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(20669..21385)
                     /gene="artQ"
                     /locus_tag="OO596_001365"
     CDS             complement(20669..21385)
                     /gene="artQ"
                     /locus_tag="OO596_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6717265.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(21392..22123)
                     /gene="artJ"
                     /locus_tag="OO596_001366"
     CDS             complement(21392..22123)
                     /gene="artJ"
                     /locus_tag="OO596_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6717266.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(22141..22869)
                     /gene="artP"
                     /locus_tag="OO596_001367"
     CDS             complement(22141..22869)
                     /gene="artP"
                     /locus_tag="OO596_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6717267.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(23087..23602)
                     /locus_tag="OO596_001368"
     CDS             complement(23087..23602)
                     /locus_tag="OO596_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6717268.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            24251..26020
                     /locus_tag="OO596_001369"
     CDS             24251..26020
                     /locus_tag="OO596_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6717269.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            26231..26554
                     /locus_tag="OO596_001370"
     CDS             26231..26554
                     /locus_tag="OO596_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6717270.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            26551..27381
                     /gene="amiD"
                     /locus_tag="OO596_001371"
     CDS             26551..27381
                     /gene="amiD"
                     /locus_tag="OO596_001371"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6717271.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(27378..28391)
                     /locus_tag="OO596_001372"
     CDS             complement(27378..28391)
                     /locus_tag="OO596_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6717272.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(28490..29920)
                     /locus_tag="OO596_001373"
     CDS             complement(28490..29920)
                     /locus_tag="OO596_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6717273.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29931..30932)
                     /gene="ltaE"
                     /locus_tag="OO596_001374"
     CDS             complement(29931..30932)
                     /gene="ltaE"
                     /locus_tag="OO596_001374"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6717274.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30969..32687)
                     /gene="poxB"
                     /locus_tag="OO596_001375"
     CDS             complement(30969..32687)
                     /gene="poxB"
                     /locus_tag="OO596_001375"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6717275.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(32820..33788)
                     /gene="hcr"
                     /locus_tag="OO596_001376"
     CDS             complement(32820..33788)
                     /gene="hcr"
                     /locus_tag="OO596_001376"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6717276.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(33800..35452)
                     /gene="hcp"
                     /locus_tag="OO596_001377"
                     /gene_synonym="priS"
     CDS             complement(33800..35452)
                     /gene="hcp"
                     /locus_tag="OO596_001377"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6717277.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYTQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(35596..36495)
                     /gene="lysO"
                     /locus_tag="OO596_001378"
     CDS             complement(35596..36495)
                     /gene="lysO"
                     /locus_tag="OO596_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6717278.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         9141 a         9057 c         9473 g         9039 t
ORIGIN      
        1 atgcgcgtgc atggatttta atggattacg ccagcggtaa agtgctggca gaaggcaacg
       61 cggatgagaa actggatccc gcgagcctga ctaaaatcat gaccagctat gtggttgggc
      121 aggcgcttaa ggccgataag attaaactca ccgatatggt gacggtcggt aaagatgcct
      181 gggcgacggg aaatccggca ctgcgtggtt catcggtaat gttcctcaaa ccgggcgatc
      241 aggtttcggt ggcagacttg aacaaaggtg tgattatcca gtccggtaat gacgcctgta
      301 ttgcgctggc cgattacgtt gccgggagcc aggagtcatt tattggtttg atgaatggtt
      361 atgccaaaaa aactgggtct gaccaacact accttccaga cggtgcacgg tctggatgcg
      421 ccggggcagt tcagtaccgc gcgcgatatg gcattgctgg gtaaagcatt gatccacgat
      481 gtgccggaag agtacgccat tcataaagag aaagagttca ccttcaacaa aattcgtcag
      541 cctaaccgta accgtctgct gtggagcagc aatctgaatg ttgatggcat gaagacagga
      601 accacggcag gtgcgggata taatctggtt gcttcggcta cccagggtga tatgcgttta
      661 atctccgtag tgctgggggc gaaaactgac cgtatccgtt ttaatgagtc tgagaaatta
      721 ttgacctggg gtttccgctt ctttgaaact gtgacgccaa ttaaacctga tgccaccttt
      781 gtgactcagc gcgtctggtt tggtgataag agcgaagtga atctcggggc aggcgaagcg
      841 ggctccgtga ccataccgcg tgggcagctg aaaaacctga aggcgagcta cacgttaacg
      901 gaaccgcagc ttaccgcacc gctgaaaaaa ggtcaggttg tcgggaccat tgatttccag
      961 cttaacggta aatccattga gcagcgtccg ctgatcgtga tggaaaatgt ggaagagggc
     1021 ggattctttg gtcggatgtg ggatttcgtg atgatgaaat tccatcagtg gttcggcagc
     1081 tggttctctt aatcttctga taaccggatg gcgcgaaacg tcatccggtt atacgtcatt
     1141 aatacatcaa cttaatacgg tgcgtctgcg cgtacttcac atactcatct tccgggcaac
     1201 aatcactcac cacaatatca aagcgtttca ggtcacccat gcgcgccgga cgcaccttgc
     1261 caaatttact gtggtcgaca accagcacat gcttttgcgc catcgacatg gcccagtgtt
     1321 ttaccggcaa ctcttcaaga ttaaaacagg tagcgccttt actgacatgc acgcccgccg
     1381 cggaataaaa agcgatatcc gggcaaaaat tattcaacgt ttgctgaaaa tcgatggggt
     1441 tgaaaatggc gttgctggcg tgaaattcac caccgcacag aaatgcgcgg caatggggtt
     1501 tctctttcag cgccagaaag gtatttagcg aataacaaac ggcggtaaaa gggatttcat
     1561 tatcaatcgc ttcaataatc cacggcgtgg tggtgccaca gtcaaaaaag agggtctgat
     1621 cgggttctac cagtgtcgca gccagttttg ccgcccggcg tttttcttcc accaggcggg
     1681 atttttgatc gcttaacagg taatggctgg cactgcgcgg ttccagaaca atatagccgc
     1741 cgagcaaaac gacgggcgca ctgtggttgt tcagatcgcg acgaatcgtc atctccgaaa
     1801 ccccaagcag ggcggcggcg tctttaagat gtaacttatc gctgcgtttt aattcttgca
     1861 gcagctgccc gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa
     1921 gcgagccgct caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga
     1981 tcggtaatgc aaaacagacg cgataccacg attgcagacg ttgatagagt ttatgcccca
     2041 tcgcttgcca gataatctgg gcactaaggc agccaatcat acctgccagc aatccaccga
     2101 gcatatccag cggccagtgg acgccaagat aaacgcgcga ccaggcaatg acgacggcca
     2161 gaaccattaa aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg
     2221 tgaaaatcac cgtaccgtga tcgctcggga atgagtcatc cgccgcatga tgcaggaagt
     2281 tatagccgat attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg
     2341 acacaaacag gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg
     2401 taagccccca caaccaaagt accgcggcca gcaacggcac cacggtaatc aaatctttag
     2461 caataaaaat cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag
     2521 agaatagaga gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg
     2581 agccaataca ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg
     2641 tcttatttcc ctcaagcggc ctgtttacgg tgggtgattg taacgggcat aggttaaata
     2701 aaacttaaag aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt
     2761 cagctacaaa attcgctttc tcgttagctg cgcttttatt aaactctgcg cgattattat
     2821 tggcgaagaa attgcatgca aaataaatta gctttcggtg ccaggcttgg acgtcaggcg
     2881 ttacttttcc ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg
     2941 atccaacccg gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct
     3001 acttcgatga ccgcgtatct ggcgggcggg atgtttttac aatggctcct ggggccgctg
     3061 tcggatcgta ttggtcgccg tccggtgatg ctggcgggtg tggtgtggtt tatcgtcacc
     3121 tgtctggcaa tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag
     3181 ggcataagcc tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa
     3241 gaggcggttt gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta
     3301 cttggtccgc tggtgggcgc ggcgtggatc catgtgctgc cctgggaggg gatgtttgtt
     3361 ttgtttgccg cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc
     3421 gccacgcgta taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg
     3481 ctgaagaacg gccgctttgt ggcgggggcg ctggcgctgg gattcgttag cctgccattg
     3541 ctggcgtgga tcgcccagtc gccgattatc atcattaccg gggagcagtt gagcagctat
     3601 gaatatggct tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa cttgctgtta
     3661 gcgcgtctga cctcgcgccg caccgtacgt gataattatg ggcggctggc cgattatgat
     3721 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
     3781 tgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
     3841 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg gaatgctgca
     3901 aatgctgatc tttaccgttg gtattgaaat cagcaaacat gcctggctga acgggggcaa
     3961 cggacagttt aatctcttca accttgtcaa cggaattttg tggctgtcgc tgatggttat
     4021 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgactgct
     4081 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
     4141 atcccttcac ctacgctggc aagatggcga gcataaggat gcgggcggta agccggagct
     4201 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
     4261 ctgactgaat caggaactac aaccactttg tatccactcg gcacatcgac ggtgacactt
     4321 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
     4381 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
     4441 tcaggtattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt
     4501 aagcacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
     4561 tgccgctgcg acgaccgcgc tgccggcatt ttccattgca aaactaaagc ctgcctgacg
     4621 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
     4681 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
     4741 gatgctgccg ttgccggtgt ggactggcac cataatatcg ccgatggcct catgtaatgc
     4801 tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa agaaaatatc
     4861 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ctgccaccgt
     4921 tttcatggca tcgtcatatt ttttgagcgt ataggcgcta ttttttccgc aggcaataat
     4981 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gtaaaggcat ccttcgacag
     5041 ctcgccatta aaaacatctt tgccttcact cactacccag ccgccgtttt cagccacaaa
     5101 ggcaatttca ttagcaattt cagggaagaa agagagcaac tgataatatt gattcccgct
     5161 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
     5221 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
     5281 aatgctcatc aactattctc catcgccgtc cgcgttttgg tatcgggttt ggcgacggct
     5341 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
     5401 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
     5461 gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
     5521 atggtcagcg ggaagcccag cgaggcaccc agtccccaga gtacaacaga caccccagcg
     5581 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagtgcactg
     5641 gcccgaacca cggcaacgcg actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca
     5701 acggtcatcc ctagggtaaa accggcataa atcagcgagc cggaagtagg gctaaaaccg
     5761 tgaccgtcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
     5821 gccagcacta caacaccaat caacagcaac tggatatcgc gataaaaagg aatgcctttt
     5881 tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
     5941 gcgatataaa taggcgcgat acctaccagc gccgccaata aaatgtgcac cgttgccgga
     6001 acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc taggctataa
     6061 aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc ggcaccttca
     6121 acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca
     6181 aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc gatcaatgcg
     6241 caggacatcg tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag
     6301 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
     6361 atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
     6421 aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
     6481 gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg
     6541 ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc
     6601 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
     6661 tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta
     6721 ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagattat
     6781 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc
     6841 tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataatatgg agctgatgta
     6901 ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat
     6961 gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
     7021 tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga
     7081 ggagattttg aggatggttg agagggttgc agggtagtag ataaatttta gataacaaaa
     7141 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
     7201 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
     7261 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
     7321 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca
     7381 gcgcagggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
     7441 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
     7501 acaagaaaca gataaccacg ggcacaagac tgacaattaa tccggcaatc aacatctgac
     7561 cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca
     7621 taaacaccat caagccgaac tctttcacca tgcttaatgc accttgcgga atgtaaccga
     7681 aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag
     7741 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
     7801 caataacaaa gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga atcgagataa
     7861 agccgatgcg atcggcgatg gtttttacgc gacgggcatc accgctgact tgtaaaacgt
     7921 cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc
     7981 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt
     8041 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
     8101 aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta
     8161 ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc
     8221 cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca
     8281 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg
     8341 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
     8401 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
     8461 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
     8521 agagctgcct gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg
     8581 gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca
     8641 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
     8701 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
     8761 cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt
     8821 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
     8881 caagtcgtaa ctttccgaga catagcccaa gcgtgaggac cacaaataat aacagaatgt
     8941 aattcccatt taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta
     9001 gtaagctgtt gaaagaaatg gtaatttacg ataatgtttt ttaccagaat tcagggcgca
     9061 gattcattca gcgcacctaa acgatagtaa agtaacaata tattttacta gtgtaatcac
     9121 attaggtatc aacggctata tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg
     9181 ctttatctta ctgaacgcca cactggcgaa aaatgtgttc gatagacgca gtgtcaggag
     9241 gaacgtgtga aacataaaca acgttgggcg ggggcaatct gctgttttgt cctcttcatt
     9301 gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct
     9361 gaaatcggct tgctgttttt cattcttcct ggagcagttg ccagtttctt ttcacagcgt
     9421 agagaagtcc tgaaacctct atttggcgca atgctggcgg caccctgttc aatgctcatt
     9481 atgcggctgt ttttttcacc gacgcgctca ttctggcaag agctggcatg gttactaagc
     9541 gcggtgttct ggtgtgcgct gggggcactg tgtttcttat ttatcagtag tttgtttaaa
     9601 ccacagcaca gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg
     9661 tccagatttt ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg
     9721 acaaaaatct gcggcacggt ttctacgggt ttacctgcct tttgttgtag atcttcttta
     9781 gtgatccctt ccgcacgaat atctacatac tgatactgaa aatcatcgcg ttcattgctc
     9841 aatttctcag ccagatcttt tgcacgcaca cagtaagggc aacccgaacg accaaaaata
     9901 acggtttgca ttatttctct cctcatagat ttatgcctgt aatgatcacg ctaaaatgta
     9961 ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa aagctataac tgttaaacac
    10021 aatacagtga aaagttttag actgaaggct cactttgcag agggaagcgt atgcgcgcga
    10081 tcggtaaatt gcctaaaggc gtgttgatac tggaatttat cggaatgatg ctactggcgg
    10141 tggcgctgct gtcggtaagc gactccctgt cgctgcctga gccattttct cggccagaag
    10201 tgcagattct gatgattttt ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta
    10261 ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa ccgtccaccg caatattcac
    10321 gttcagaaag agaaaaagat aatgacgcca accattgaac ttatttgtgg ccatcgctcc
    10381 attcgccatt tcactgatga acccatttcc gaagcgcagt gtgaggcgat tattaacagc
    10441 gcccgtgcga cgtccagttc cagttttttg cagtgtagta gcattattcg cattaccgac
    10501 aaagcattac gtgaagaact ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg
    10561 gcggagttct gggtgttctg tgccgacttt aaccgccatt tacagatctg tccggatgct
    10621 cagctcggcc tggcggaaca actgttgctc ggtgtcgttg atacggcaat gatggcgcag
    10681 aatgcattaa ccgcagcgga atcgctggga ttgggcgggg tatatatcgg cggcctgcgc
    10741 aataatattg aagcggtgac gaaactgctg aaattaccgc agcatgttct gccgctgttt
    10801 gggctgtgcc ttggctggcc tgcggataat ccggatctca agccgcgttt accggcctcc
    10861 attttggtgc atgaaaacag ctatcaaccg ctggataaag acgcactggc gcagtatgac
    10921 gagcaactgg cggaatatta cctcacccgt ggcagcaata atcgccggga tacctggagc
    10981 gatcatatcc gccgaacaat cattaaagaa agccgcccat ttattctgga ttatttgcac
    11041 aaacagggtt gggcgacgcg ctaaaaccgc cacgtcgatg tatgatacgc gggcttttga
    11101 ccaggtctga cagagaggtg cagggtgaaa attgccatat tgtcccggga tggaacgctc
    11161 tattcgtgta agcggctgcg tgaagccgct atacagcgcg gtcacctggt tgaaattctt
    11221 gatccgcttt cttgctacat gaacataaat cctgcggcgt cttctattca ctacaaaggc
    11281 cgcaagttac cccattttga cgcagtgatc ccgcgtattg gcaccgccat taccttttat
    11341 gggacggcgg cactgcgcca gttcgagatg ctggggagct atccgctcaa tgagtcggtc
    11401 gccattgccc gggcgcgtga caaattgcgt tccatgcaac tgctggcgcg tcagggcatc
    11461 gacctgcctg tcacgggcat tgcgcattcg ccggatgata ccagcgattt aatcgacatg
    11521 gtcggtggtg cgccgctggt ggtcaagttg gttgaaggca cgcagggaat tggcgtcgtg
    11581 ctggcggaga cgcgtcaggc ggcggaaagc gtgattgacg ctttccgcgg tctgaacgcg
    11641 catattctgg tgcaggaata tatcaaagag gcgcaagggt gcgatatccg ctgtctggtt
    11701 gttggcgatg aagtggtcgc tgcgattgaa cggcgggcga aagagggcga ttttcgttcc
    11761 aatttgcatc gtggcggcgc ggcaagcgtc gccagtatca caccacagga gcgtgaaatc
    11821 gcgataaaag ccgcgcgaac gatggcgctg gacgttgctg gtgtggatat tctgcgtgct
    11881 aatcgcgggc cgttggtgat ggaagtgaat gcgtcgccgg ggctggaagg aatagaaaaa
    11941 accactggta tcgacatcgc gggtaaaatg atccgctgga tcgaacgcca cgctacgaca
    12001 gaatattgcc tgaaaacggg tggttagtcg caatcacatt actgatcatg gttttgcctg
    12061 cgctttttgc gtaagctgtg ccggtctttt tatcgaaaga ggttgtacaa aattatgaca
    12121 tcgctggtcg ttcctggtct ggatacgctg cgtcaatggc tcgatgacct ggggatgagt
    12181 ttttttgaat gtgataactg tcaggctctg catctgcccc atatgcagaa tttcgacggt
    12241 gtctttgatg ccaaaatcga tctgatcgat aacacgatcc tgttttctgc catggcggaa
    12301 gtccgacctt cagccgtatt gccgctggcg gcggatttat ctgccatcaa tgccagttcg
    12361 ctgaccgtga aagcatttct tgatatgcag gatgataatc tgccaaagct ggtggtttgc
    12421 cagtctttat ccgttatgca gggcgtaacc tatgagcagt ttgcatggtt cgtgcgtcag
    12481 agcgaagagc agatttcgat ggtcattctt gaagctaatg cccatcaact gctgttaccg
    12541 actgacgatg aagggcaaaa caacgttacc gaaaactatt tcctccactg ataactcctt
    12601 tcgagcacgc agtcgctggt gcagtggctg cgcgctgcaa aattatctgc tgtttttaac
    12661 cttttcttaa agattatttc acttctcttg tgtcgatttg gctttatcac atagagcaaa
    12721 tacgcataaa aatttgttaa ataccgtttt ttaatccgag ctatagtctc aaaccctggc
    12781 taaagttatt cttgcgatgc ttttatatag cgagcagtgc tggccgggag agagttctct
    12841 tttcttacac cccgccgata aaaaatatgc acgtttattg catatctttt agtgtgacaa
    12901 cttttgttcg tttgttaacg aactttcaga aggaaagaga tatgaccgcc ttaaataaaa
    12961 aatggctttc gggtctggct gcgggtgctc tgatggccgt ctctgtcggc acgctcgcgg
    13021 ctgaacaaaa aacactccac atttataact ggtctgatta tatcgccccg gacacggtgg
    13081 ccaattttga aaaagaaacc ggtattaaag tcgtctacga tgttttcgac tctaacgaag
    13141 tactggaagg caaattaatg gcgggtagca ccggctttga cttggtggtt ccatctgcca
    13201 gctttcttga acgccagttg actgcgggag ttttccagcc gctggacaaa agcaaattgc
    13261 cggagtggaa gaatctcgac ccggagctgc tgaagctggt cgccaaacac gatcccgaca
    13321 ataaatttgc tatgccctat atgtgggcga cgaccgggat tggctataac gttgataaag
    13381 ttaaagctgt gctgggcgaa aacgcgcccg tcgatagctg ggacttgatc ctcaaacctg
    13441 aaaatctgga aaaactgaaa agctgcggtg tctctttcct ggatgcgcca gaagaagttt
    13501 ttgctaccgt gttgaattat ctcggcaaag atcccaacag cactaaagcg gatgattaca
    13561 ccggaccggc aaccgatctg ctgttaaagc tgcgcccgaa cattcgttat ttccattcat
    13621 ctcaatacat taacgacctg gcaaacggcg atatttgcgt cgctatcggc tgggcaggtg
    13681 atgtctggca ggcgtcaaac cgcgcgaagg aagcgaagaa tggagtgaat gtctcgttct
    13741 cgattccaaa agaaggggcg atggcgttct ttgatgtatt cgccatgcct gcggatgcca
    13801 aaaacaaaga cgaagcctat cagttcctga attacctgct gcgcccggat gtagtagcgc
    13861 atatttccga ccatgtgttc tatgccaacg ccaataaagc agccacgccg ctggtgagtg
    13921 cggaagtccg tgagaacccg ggtatttatc ctccggcgga cgttcgtgcg aagctattca
    13981 ctctgaaagt acaggatccg aaaatcgacc gtgtgcgcac ccgcgcgtgg accaaagtga
    14041 agagcggaaa ataatccgca gccttagatg ccgggcgggc gcaccacacc agccggcaat
    14101 tcgcaccatc atggtgcgct tgcacacatt caatgccgga gagcagccgt gaatgacgct
    14161 atccctcgcc cgcaggcgaa aacccgtaag gcgctgacgc cgctattaga aatccgcaac
    14221 ctcaccaaat cctacgatgg tcagcatgcg gtcgatgatg tcagcctgac catctacaaa
    14281 ggtgaaatct tcgcgctgct gggcgcatcc ggctgtggca agtccacgct gctgcgtatg
    14341 ctggcaggtt ttgaacaacc ttctgccggg cagataatgc tcgatggcgt cgatttgtca
    14401 caggtgccgc cttacctgcg ccccatcaat atgatgtttc agtcttacgc gctgtttccg
    14461 catatgaccg tggaacagaa catcgctttt ggcctgaaac aggacaaact accgaaagcg
    14521 gaaattgcca gtcgggtcca tgagatgctc ggcctggtgc acatgcagga gttcgccaaa
    14581 cgcaaaccgc atcagctttc cggtggtcag cgacagcgtg tggccctggc ccgaagcctt
    14641 gccaaacgcc cgaaactatt actgctcgat gagccgatgg gcgcgctgga taaaaagctg
    14701 cgtgaccgga tgcagcttga agtggtggat attctggagc gcgtcggtgt gacttgtgtg
    14761 atggtcaccc acgatcagga agaggcgatg accatggcgg ggcgtatcgc cattatgaat
    14821 cgcgggaaat ttgtccagat tggcgagccg gaagagatct acgagcatcc aactacccgc
    14881 tacagcgctg aatttatcgg ttcggttaat gtctttgaag gtgtactcaa agagcgtcag
    14941 gaagatggcc tggtgcttga ttcgccgggg ctggtgcatc cactgaaagt cgatgcggat
    15001 gcttcagtgg tcgataacgt gccggtacac gtagcgctgc gcccggaaaa aatcatgctt
    15061 tgcgaagagc cgcccgccaa tggttgtaac ttcgcggtgg gggaggtgat acacattgcc
    15121 tatctcggcg atctttcggt gtatcacgtt cgactgaaaa gtgggcagat gatcagcgcc
    15181 cagctacaaa acgcccatcg tcatcgtaaa gggttaccga cctggggcga cgaagtgcgt
    15241 ttgtgctggg aagtggacag ctgtgtggtg ctgacggttt aaggagcaaa gatgagtaca
    15301 cttgaacctg ctgcccagtc gaaaccgccg ggcggattta agctgtggtt gtcgcagctg
    15361 caaatgaagc atgggcgcaa actggtcatt gcgttgctat atatctggtt gatcttgctg
    15421 tttctgctgc catttctgat tgtctttaaa ataagcctgg gagagatggc gcgcgctatt
    15481 ccaccttata ccgaactgat ggagtgggct gacgggcaac tttccatcac tcttaatctc
    15541 ggtaattttc tgcaactgac cgacgatccg ctctattttg atgcttatct ccagtcgtta
    15601 caggtggcgg cgatttcgac attttgctgt ttactgatcg gctatccgct ggcgtgggcg
    15661 gtggcgcaca gtaagccttc gacccgtaat attttattac tactggtgat cctgccgtcg
    15721 tggacctcgt ttctgatccg cgtttatgcc tggatgggaa tattaaaaaa caacggtgtg
    15781 ctgaataatt ttctgctgtg gctgggggtt atcgatcaac cgctgaccat tctgcatacc
    15841 aatctggccg tttatatcgg cattgtttac gcttacgtgc cgtttatggt actgccgatt
    15901 tataccgcgt tgattcgtat tgattattcg ctggtggaag cagcgctgga tctcggtgca
    15961 cgaccgctga aaacgttctt tactgtgatc gtgccgctga ctaaaggtgg gattattgcc
    16021 ggatcgatgc tggtgtttat cccggctgtg ggcgagtttg tgatcccgga actgctcggt
    16081 ggcccggaca gcatcatgat cgggcgcgtg ttatggcaag agtttttcaa taaccgcgac
    16141 tggccggtgg cctcggcggt agcgatcatc atgttgctgc tgctaattgt gccgataatg
    16201 tggttccaca aacaccagca aaaaagcgtg ggagaacacg gatgaataat ttaccggtag
    16261 ttcgttcgcc ctggcggatt gtgattttgc tgctgggctt tacctttctc tacgcgccaa
    16321 tgctgatgct ggttatctat tcgtttaaca gctcgaagct ggtgacggtg tgggccggct
    16381 ggtcaacgcg ctggtatggt gagttattgc gcgatgacgc gatgatgagt gcggttggtt
    16441 taagcctgac aattgctgcc tgtgcggcaa cggcggcggc gatcctcggg actattgcgg
    16501 cggtggtgct ggtgcgtttt ggcaggtttc gcggatcaaa tggttttgcc tttatgatca
    16561 ccgcgccgct ggtgatgcca gatggcatca cgggcttgtc gctgttgtta ttattcgtcg
    16621 cgcttgctca tgccattggc tggcctgcgg accgcggtat gctcaccatc tggctggcgc
    16681 atgtcacttt ttgtacggct tatgtggcgg tcgttatttc ttcaagattg cgggaactgg
    16741 atcgctcgat agaagaagca gcgatggatc tcggtgcgac gccgctgaaa gtgtttttcg
    16801 tcattacgct accgatgatc atgcccgcga tcatttctgg ctggttactg gcttttactt
    16861 tgtcgcttga tgatctggtg atcgccagct ttgtttccgg gccgggagcc accacgttac
    16921 cgatgctggt cttttccagc gtgcggatgg gagtgaatcc ggaaatcaac gcccttgcaa
    16981 cgttaattct cggtgcggtc ggaattgtcg gatttatcgc ctggtatctg atggttcgcg
    17041 cagaaaaaca gcggatacgc gatatccagc gtgcaagaca tggctgaaga cactaaaatt
    17101 tgccaacctg gctacataat gccgcgcatg ccgcggcatt gttttcatgg aagacgaaac
    17161 gttgggattt tttaagaaaa catcttcatc tcatgctcgc ctgaatgtgc ctgcgctggt
    17221 gcaggtggcg gcgctcgcca ttattatgat ccgtggcctc gacgtgctga tgattttcaa
    17281 tacgctgggc gtgcgcggta ttggcgagtt cattcatcgc agcgtacaaa cctggagttt
    17341 aacgctggtc tttttaagca gtctggtgct ggttttcatt gagatctggt gtgcgttttc
    17401 actggtgaaa gggcgtcgct gggcgcgctg gctatatctg ctgacacaaa tcaccgccgc
    17461 aagttacttg tgggcggctt cgctggggta cggttatccg gagctgttca gcattcccgg
    17521 tgaatcaaaa cgtgaaatct tccatagcct gatgctgcag aagctgccgg atatgctcat
    17581 cctgatgctg ctgttcgttc cctcgaccag tcggcggttc ttccagttgc aataatgtgt
    17641 ataatcgtcg cccctgatga tgtgaaggtc aatgtatgca gtgcgcactt tacgacgcgg
    17701 gtcgctgtcg ttcctgtcag tggataacgc agccgattcc agagcaactc tccgctaaaa
    17761 ccgccgatct taaaaatctg ctcgccgatt ttccggttga ggaatggtgc gcgccggtgt
    17821 caggcccgga acaagggttt cgtaataaag ccaaaatggt ggtgagtggt agcgttgaaa
    17881 aaccactgct cggtatgctg catcgcgatg gcacgccgga agacctttgt gactgcccgc
    17941 tttatcctgc ctcttttgcg cccgtttttg cggcgctaaa accgtttatc gcccgtgcgg
    18001 ggttaacgcc ctacaacgtg gcgcgtaagc gtggcgaact gaaatacatt ctgctgactg
    18061 aaagccagag cgatggcggc atgatgctgc gctttgtact gcgttctgaa accaaactgg
    18121 cacaactgcg taaggcgctg ccgtggttac aggaacaact accgcaactg aaagttatta
    18181 ccgtcaatat tcagccggta catatggcga ttatggaagg ggagacggag atctacctga
    18241 ctgaacaaca ggcgctggca gagcgtttta atgacgtacc gctgtggatc cgtccgcaaa
    18301 gtttcttcca gactaatccg gcggtcgcca gccagctgta cgctaccgcg cgcgactggg
    18361 tacggcagct gccagttaaa catatgtggg atctgttctg cggtgtgggt ggctttggtt
    18421 tacactgcgc gacgcctgac atgcagttaa ccgggatcga aattgcatca gaggccattg
    18481 cctgtgcaaa gcagtcagcc gctgaactgg gcttaacgcg tttgcaattt caggcgctgg
    18541 actccactca gtttgccacc gctcaggggg atgtgccgga gctggtgctg gttaacccgc
    18601 cgcgccgcgg cattggtaaa ccgctgtgtg attatctctc aacgatggca ccgcgtttta
    18661 ttatctactc cagctgtaac gcccaaacca tggcgaaaga tatccgcgaa ctgccaggtt
    18721 accgcatcga acgggtacag ctttttgata tgttcccgca caccgcgcac tatgaagtga
    18781 tgacgctgct ggtgaagcaa taaaaaagcc gcaggtgcgg cttcagattg ctgatcaggt
    18841 gcgctttgtt catgccggat gcggcgtgta cgccttatcc ggcctacaaa atcatgctaa
    18901 atcaatacat tgcagagttg atgtgggcca gataagcgca gcgcatcagg caatcttact
    18961 tttgtcatca gtctcaatta ctgcgggaac cactggtcat tgattttctg atatgtaccg
    19021 tcagctttaa ttgctgccag cgcgttattc agtttttcca gcagagcttt gttatccgga
    19081 cgtacagcga tgcccagacc ggtgccgaag tattgcggat cggtcacttt ttcagtagca
    19141 acgcccagtt gcggattggt tttcagccat tcgtttacca ccgctgtgtc accaaatacc
    19201 ccatcaatac gaccattttt cagatcgata aaggcattct gataactgtc ataggagaca
    19261 gttttcactt cggggtgctg atcctgaata tatttctggt gcgtggtgcc gttttccatc
    19321 ccaatacgtt tgcctttcag atcggcaaac gttttgtagg tatctttttt ggcaatcacg
    19381 acggctgagt tttcatagta gggcgtggta aacgatacct gcttgctacg ctcgggcgtg
    19441 atatccatac cggagattac ggagtcatat tttctgaatt tcagtgacgg gatcaggctg
    19501 tcgaacgcgt gattagtaaa agtacattct gcctgcattt gtttgcacaa ggcttttgcc
    19561 agatcgatat caaagccgac aatctcatta ttagcaccta tagattcaaa gggtggatag
    19621 gtggctgaaa cgccaaaatt gattttctct gcggcagaag caccgaaagt aaaggaagca
    19681 agtaaagcgg caagaactaa ctttttcatg atggaactcc cgtctgtcaa tcttatgatt
    19741 tttggccgtg tctgcggcat gggataacaa tgccatcaag tgaatttata tgcaataaat
    19801 atgattaaat aatttaaatg aaataaaaaa agacggataa cttagtgagt tgtccgtctt
    19861 cattataaga atttatgcac tatgtaggcc ggataaggcg tccacgccgc atccggcaca
    19921 ggcaccgtgc tgatgtctga tgcgacgctg gcgcgtctta tcagacctac aaaccccccc
    19981 ggcgaatgta cgcagctaca ttaattccgc cgttcgaatg ccagtgcttt gcgctcgatc
    20041 agacgcatca tcagcgtcag caggccgttg acgaccaggt aaataatccc tgccgcaccg
    20101 aacaccatta catcgtaggt gcgtccgtac aacaactggc tgtatcccat cacttccatc
    20161 agcgtaatgg tgtaagccag agaggtactt ttgaacacca gcaccacttc gttggaataa
    20221 gaagagagcg agcgtttaaa ggcatacggc agcaggagcg ccagcgtatc ttttttgctc
    20281 attcccaggg cgctacagga ctgccactga ccttctggga tcgcacgaat tgcaccgtaa
    20341 aacagctgcg tggtatacgc cgcactgttc agcgataacg caatcagcgc acataaccac
    20401 ggttctgaca acaaatgcca cagtgccgga tactcctgca acgtcggaaa ctggcccggc
    20461 ccgtaataaa tcaggaagat ctgcaccagc agcggcgtac cggtaaacag cgtgatataa
    20521 ccccgcacca gccacaccag caccggcgtt ttcagcgtca ggatgatggt aaaaatcaat
    20581 gccagaatca gtgccacaat cagcgaggca acggttagcg tcaggctggt gtgtagccct
    20641 ttcatcagtt cgggtaaata ctcaaacatt agctgggcct ccgctcaaaa cgtgtcgcgc
    20701 gcaggtcaat gcgtttgaga atgtactgac tgagcagggt gatcaccagg taaatcgccg
    20761 ccgccacaat gtaccaggta aatggttcct gggtacgagt agcgatgctt tttgtttgca
    20821 gcattaaatc attcacacta atcaaactga ccagcgcggt atctttcagc agcaccagcc
    20881 actggttacc gaggccaggc agcgcatgac gccacatctg cggcatcacc agacggaaaa
    20941 agatagccga tttcgacagc cccagcgcct gaccagactc ccactgaccc accggcaccg
    21001 ctttcaacgc gccccgaagc gtttgcgagg cataggcggc atacagcagt gacagagcga
    21061 tgacaccaca aaggaacggg ctaacgtcga agttctcaat gtccatctgc actgggatct
    21121 gcacgaaccc aagattgata gtgaagccat ccgaaagcgt cagcagcagc tgcgaggagc
    21181 caaaatagat aaacagcacc accagaattt ctggcaggcc acgcagaatg gttaccagcg
    21241 ctgaacctgc ccacgcgaca ggacgccatt ttgccgactc ccataccgca aagaacatcg
    21301 ccagcgccag cccgacaatc aatgcacaaa cggcaaggcc gacggtcatc ccggcggcgc
    21361 ttgctaaagg aaaaaattca ttcatcagga attacttctg gaaccatttg ttgtagatgg
    21421 tttcgtaagt gccatctttc ttcacttttt ccagcgcagt gttgagtttc tgctgcagct
    21481 cagtgttgcc ctgacgtacc gcgatgccga ggccagtgcc gaagtaatct ttatcggtca
    21541 ctttgtcgcc caccgccgcc agtttcgggt tatctttcag ccactcagtg accactgcgg
    21601 tgtcaccgaa gacgccgtcg atacgcccgt tttgcagatc cagttttgcg ttctggtagc
    21661 tgtcatacgg aacggtagtg atttccgggt gcttatccat aatgaatttc tggtgtgtcg
    21721 tcccgttctg tacgccgact tttttgcctt tcagctgatc aacactggtg tatttgcctt
    21781 gctgacccac aaacagggca gagttgtcat agtacggggt ggtaaacagc acctgctttt
    21841 cacgctccgg agtgatatcc atgcccgcca tcacggcttc tacgcgacgg aatttcaggc
    21901 ttgggatcag gctgtcaaac gcctggttag agaaagtgca ggttgcatca atctctttac
    21961 acagcgcttg tgccaggtcg acgtcaaaac caacgatctg gttgtttgca tcaatcgatt
    22021 caaacggagg ataggatgct tcggtagcaa aacgaatggt ttcggcagct gtggcggaaa
    22081 gactaaaacc tgcaattaac gcggcaatca gaactttttt cattgttgtt atcccgaatc
    22141 ttagtgagag agatagtttt taaatgcttc ggtttgcggc tcggtaaagc agctcgcgtc
    22201 gccttgttct acgatatgac cattttccat atacaccact cggctggcgg ttttacgcgc
    22261 cacttcaact tcgtgggtga cgatcacctg ggtaatattc gtttctgcca gctcacgaat
    22321 gatgctgacg atttgtgccg taatttccgg gtccagtgcg gcggtcggtt catcgaacag
    22381 cagtacctgc ggttccatca tcaacgcacg ggcaatagca acacgctgct gctgaccacc
    22441 agaaagatgc agcgggtaac gatcgctata aggtttgaga cgcagacgtt ccagcagttt
    22501 ttctgcacgg gccagcgcct gatctttact caaccccagt acacggcagg gcgcttcaat
    22561 caggttttgc tgcacggtca gatgcggcca caggttgtat tgctgaaaca ccatgccaac
    22621 gttacgacgc aaatcgcgaa tcgctttgtc agagggtgtt ttggtgaaat cgaaatggtt
    22681 gcctgcaatg ttgagcgtac cggagcgtgg catctcaagc agattgagta cacgcagcag
    22741 cgagctttta cccgcgccgc tggggccaag taacaccagc gtttcgccct gtgggcaatc
    22801 cagcgtgata tcgaacagcg cctgatgcgc gccgtagaag caattaatgc cgtttaattg
    22861 aatactcatt gacactcgta tactggcagt ctgatagcta ttgaggtcga agatagtacc
    22921 tttgacagaa taattatgca atatttctgc tttaaaagtt aaaagcaaag cgcattattc
    22981 aataaacata gcacaaaata acgggggcgg tggtcggcga gcataaatgt cggcattcct
    23041 cacgaaatgc cggacaattt acggggttta ttggttgatc aaggcgttag cgattctcga
    23101 tggactgacg gagcgtaccc gccgtggcat gaacgctgcc gcctaagtag cgcacatcgt
    23161 cgatgaccca acactggcct tcctgaatca ttaacacttc atcctgccaa ccctggtcac
    23221 cctgtttgag atctacgcgc aatggaatgt tacgggcatc acgattaggg atagtcgatg
    23281 cactggcaac gtgggcgcta tctggcaagg tggttcgact ggagaacgga tcgctgctta
    23341 acaattcacg atgactatta tcgcggctgg catcgctaag aagggttgcc agtttgtcac
    23401 tcaaataggg gcgcagggcg gtaatgtcat tgctgcgatg ttgaatgcgg tagtcataga
    23461 attgttgagc cacgctgtca ggccccccct ggacacagga accggtgcgg ggaccgttat
    23521 ctttataagc tggcgtgacg gtggtacagg cactcagcag cagtgcacat ggaataagta
    23581 gagatagtga acggtagcgc ataatcattt ccttattagc ttgctgtctg cgtcacacta
    23641 cgaccaaacg ctatcagatt atttaataca ttgtttttaa tacaattctg aaacaatctg
    23701 aacacctgat tgggtttgag tccattgctg gctatccact caaggttgct cgtagctgac
    23761 ttacttaaat tacccaaaca atagacagtg taaaccattt gtgaggttga gcgtgttata
    23821 tggctgaaat gatcatttaa ggtaaatatg atagttctga aatggtggta taaatcctcc
    23881 tgtagaggtt aagagcatct gcaagaagcc agtttcacat ttataacgcg tgataaatga
    23941 acgcggttat tgtctaatca ttacaaaatt tttcacatgc gataagagat cctgcgatta
    24001 agatgattgc caaaattacg ttgtattttt gaaaatgagc gaaatttgat agtcccagct
    24061 ttgactctcg ataatattat ttatgctaca attgtggcta aagaatttaa gctaatgaat
    24121 tttagcgaag aataaattaa gttgtttcag tatctcgtta attaatatga atataaaagg
    24181 cgttgtgctt ttataattat aaagcacgag gagttcggtg gattatcgtt tttcttgggt
    24241 gatttttaat atgataaaca atatatctga tcaagcatca tcatttccag gcacacaact
    24301 aaatcaaagt gataatttcc tggattcact gagggaattt ttcgcgatat taaattccag
    24361 tcgtaagggt gagttgtcta catgggatac catctacttg catctgatat tagcaataaa
    24421 tgctgacagt gatctcataa aaaatgatgt gttgctcgcg gaaaatatgc catcagcgaa
    24481 ttaccaattt aatacttttt ttagtaatgc tttcgagact gatgttaaaa aatatctggg
    24541 taaatcagaa gataacgaag ttgaaataaa ggcgggtaat gaaagaatct ctattggaat
    24601 acgtaatgta tcgaatggga aacttgaaag acaacagttt cttttccctc tggactatga
    24661 gaataaatta caagatcaat tagacaagca ttttacgatt gaatcgcacc cattattata
    24721 caggcatacc attggtagta aaattgcgaa tgtgattttt gaaaaattat acagccgtat
    24781 tgactttaac aaagaacaat atatatcctt tataaagggc gcgtttattc atttttatga
    24841 ttatagcagg cgatatgcaa ttagtgagaa tatcgaaaaa gacgctgtaa caaacaatat
    24901 cgcattgatg aacacttttt atgacagtga taatacgtca ggtgaggttt taaataatga
    24961 ttttacagaa gaagagccat ttgaaactgc gttagatgta gagcatacaa tagtgcttgg
    25021 atttgctgat gataattttg aaacaaaacc tgttcattat caggatttat taacacgatt
    25081 tagtgccttt caagataccg tgtttaatct gtttcctgaa atgcgctctt cgcattacca
    25141 cgatatttgt agcgtatgtg tggatatgac aaagggaacg caatgcatga ttcacttgat
    25201 ggtgaatgaa gaagttttca tgtctctgcc tgttccagtt gccacgatgg tacgtgaaga
    25261 tgcaagtaat ttagtcaatc ttaaaacact actcaatgat ggttttttca ttaaatatag
    25321 tcattttaat gacgtggcat taataaagca aaacataagt aatctctact tatcacacac
    25381 tgttgtgaat gaaagcatat tgaaaaaatg ttgttttgaa aacggcagtt taggtgatgt
    25441 taaaataaca aatagcaata taataaataa tgtattcaaa aatgtttcgt ttagatctgt
    25501 taaaattaac aacgtaaata ctcattcttt aaaatttatt aattgccatt tttttaatgt
    25561 tgatatgata agagttaatt tcagcaaatg tctttttcat gactgtagta tgcatggtgt
    25621 caagataaaa ccatggttgc cagtaaaatg gacaaaagag ttaataaatg attatttgta
    25681 tggctgttta ctatctctgt actctatctg tgcaagggat atatataata tgagcgccgg
    25741 gaataacgtg aaggttgctg cggatgcttt ccttgaaatt attttctctc ttaagaataa
    25801 atattgcatt aaactattaa gcgctcagga acgggcgttt atttacgaat ttgcaagaat
    25861 gatcttcgct tatattaatg acaaaagcat tgagatctta ttactttctt gttttgcggc
    25921 gtctgaccaa aaagcaatcc agaggtatat acctcagtca caagatgggg aagactttag
    25981 gagtcattta caatataagt tgcctctttc cgctcattaa tgttgttggt gcaacataat
    26041 aagacaggta agcggtaccc tataatgaaa ccaatatcgg gttatgcaac ggataatcgt
    26101 aaagacttaa gtgggtgttt ttgacacaat gagatgattt cctaaactga tcaaaacctt
    26161 cttcccaaca tggcatatca gttcgataat gctttagaga tcgaaggctt aacaaacaag
    26221 gagatcgatc atgcaatttt ctacaacccc aactctggaa ggccagacca tcgttgaata
    26281 ttgcggtgtg gtgaccggcg aagcgatttt aggtgccaat attttccgtg atttctttgc
    26341 cggtatccgc gatatcgttg gcggacgttc cggtgcgtat gaaaaagaac tgcgtaaagc
    26401 acgggagatc gcctttgagg aattaggctc ccaggcgcgg gcgctggggg ccgatgccgt
    26461 cgtcggtatt gatatcgact acgaaacggt cgggcaaaac ggcagtatgc tgatggttag
    26521 cgtcagcggt acggcggtga aaacgcgtcg atgagaagag tcttctggct ggtcgctgcc
    26581 gctctgttat tggcagggtg tgcaggcgaa aaaggcattg tcgagaaaga gggatatcag
    26641 cttgataccc gacgccaggc gcaggcggcg tatccgcgca ttaaagtgct ggtgatccac
    26701 tacaccgcag atgattttga tagctcgctg gcgacactga ccgataagca ggtcagctcg
    26761 cattatctgg tccctgcggt accaccgcga tacaacggta aaccgcgcat ctggcaactg
    26821 gtgccggaac aagaactggc ctggcatgcg gggattagcg cctggcgcgg ggcaacgcgc
    26881 cttaacgaca cctctattgg cattgagctg gaaaaccgtg gctggcaaaa atcggccgga
    26941 gtgaaatatt ttgccccgtt tgaaccgaca cagattcagg cgcttattcc actggcgaaa
    27001 gatattattg cccgttatca catcaagccg gaaaacgtag tggcacatgc ggatatcgca
    27061 ccgcagcgca aagacgatcc ggggccatta tttccctggc agcaactggc gcagcagggg
    27121 attggtgcct ggccggatgc gcagcgggtt aacttttacc ttgccgggcg cgcgccgcac
    27181 acaccggtag ataccgcgtc attgctggag cttttggcgc gctacggtta tgacgttaaa
    27241 cctgatatga caccgcgcga gcagcggcgc gtgattatgg cattccagat gcatttccgc
    27301 ccgacgttat ataacggcga agcggatgca gaaactcagg cgattgccga agcattgctg
    27361 gagaaatacg ggcaggatta gcgcggcagt tttccgtggt cgcgtagcca ggcggcagtt
    27421 ttctcgatac cttcatctag ggtgatgatc ggctgataac ctaactcttc ctgcgcacgc
    27481 gtaatatcca gcgtaaagtc aaaattcaac ttggagacgc cgtagtgggt cagcggcggc
    27541 tcttttgctg acttgcggcc taaacgctcc atgctgcggg cgatcatatc cagcatcggg
    27601 taggggacgg aacgaatacg acagtcaata ttcaactcgt cgatcagctt ctgcacgatg
    27661 ctgcgcagtg tgcgatgctc gccgttggtg atattgtaca cacgcccgga aggtagctta
    27721 tcgcaggctt cctggcttgc cagccacatt gcgtgcacgg tattttcata gtaggtcata
    27781 tcgaccagcg cactgccgcc atgcggtaac agaatactgc cgtagtggtg catcatatgc
    27841 gccagacggg gaataaagac tttatcgtgc ggtccgaaca gactttgtgg gcgcagaata
    27901 gtaaagcgcg tttgtggatt cgcctgcgaa agcatattga tcacttcttc gctggctgct
    27961 ttgctgcggg caaactcgtt ggcgaagcgg tgagggcgaa aatcttcttt aatatcgcga
    28021 tggtggtgat aatcgaagta cagggagggg gaagagatat gaataaagtt acgcacaccc
    28081 caggcgacag cccattcacc caggcggcga gtggcgcgaa cgttagccag atcgaaagcc
    28141 tgttgtgtcc cccagggtga ggtaaagctg gagcagtgcc acagcgtatc aatgcccgcg
    28201 agcatcactt tagcttgtga tgaaaccagc tcggtcagat ccgccggaac aaactctgcg
    28261 cccatttttt ccagcaattt gcccattgcc tcgttgcgac cggtcgctcg cacgctgatg
    28321 cctttctggc ataaaaactc taccgcgttt cgacctaagc cgctggtggc gccggtaacc
    28381 agtaccttca tatcaatcca ctgttgttga gaaaatgacg tgcgcattct tccgtgattt
    28441 cccccatgat gcaatgggaa acatgaaaga ataacgcagg ttttgtcgat taatctgtgc
    28501 tttgttctgc cagtctggcg atttgttttg ccattccgcg aaaaataaac agatgcgcgg
    28561 ggatcatcaa taaccagtaa aacagccccg gcataccgtg cggatgccag aaagcgcgga
    28621 catcgatagt acgatagtcg cctttatctt ccaggctaaa acacagtcgt cccagccccg
    28681 acgctttcat gccaaataac aacgtaagtt gtttttccgg ttcaacgaca atcactttcc
    28741 agctatccac cgcatcgcca gtctgtaaat attcgcgctc cgggcggcct ttcgccagct
    28801 tatgaccgat cgcgcggtcc atcaacgccc gtgtctgcca caaaatattg ccaaagaaat
    28861 aacgctcttt accgccgatt tggttcacta cctgccataa agcagcaagg ctggcggacg
    28921 ttttaacggt aaaccccgcc tgtttggcaa aataaccgta ctccggtcgc cagcgggcaa
    28981 aggcctgagc gtcgtagccc cagtcgctgg agttgaccag tttttcctcc tctttcaacg
    29041 tgctacgtac cgcgtcatcg aaagcgatca gccgttgtgg gatgagtgca cgtagcgcgg
    29101 tatcatccgc cagcagatcg tgtttcagcc cctgaatcaa cgccctggcg gtggtgggcg
    29161 gtacggaagt aatcacattg agaaaccaca ccgaaatcca gcgggtgggg agggggatgg
    29221 ggatcaacca gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt tgctgataac
    29281 tgagcacctc tggtccggcg gcttcgaaga tgcggtgttc gctggccgga tgatctaaca
    29341 acgccaccag atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt gaacgtaccc
    29401 agcgtggcgg cgttaacact ggcaggttgt agaccatatc gcgcatgact tcgaacgccg
    29461 ctgaacctgc gccaacgata attccggccc gaagttcggt cacaggtaca ttcgcttcac
    29521 gaagaatgtc cgccgtagcc tgacgagcac gcagatgatc cgactgctca tgtggcgggg
    29581 cctgcaacga actgagaaag attaattgct taactggtac ttcacgtagc gcatcgcgga
    29641 cgttgagagc cacctggcgc tcctgagcga taaaatcgcc gccttcgccc atgctgtgca
    29701 ccagaaaata gacggtatcg atatcctgca acagggccgg aaggttatcc ggccagctga
    29761 gatcgacttt atggcaactg acgtttgcca gttgcagctt tgcaagcctg tcgacatgac
    29821 gtgccgccgc caggatctga tgcccttgct ggctgagtgt gcgcaccaga tgctgaccaa
    29881 tgtagccact ggcaccgaga actaaaatgc gttgcggcac gtctctctcc ttaacgcgcc
    29941 aggaatgcgc gccagtgggc ggcgacttcc gccagttgtt cgcgcgagac gtcaagatgc
    30001 gtcaccaggc ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt catgtattcg
    30061 cctaacgcgg cagcattttc ttccccgacg cgaacaaaca gcatattagt gtcctgacgc
    30121 atcacatccg cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt gtcgtgatct
    30181 tcctgcaacc gtgcgacgtt atttttcagg gcatacatcc cggcggcagc cagaatcccg
    30241 gactgacgca tcccgccacc ggccattttc cgccagcgga tggcacgttt aatgtaatca
    30301 cgattaccga cgagtaatga accgactggc gtcccaagac ctttcgacag gcaaatggtg
    30361 aacgaatcac aatattgcgt gatctctttc agttcgcagc cgtaagccac cacggcatta
    30421 aagatgcgcg caccgtcaac atgcagcgcc agattgcgct tgcgggtaaa ttcccatgct
    30481 tctttcaggt attcacgcgg cagcactttg ccgttgtggg tgttttctag actgagtaat
    30541 ttggtgcggg cgaaatggat atcgtcgggt ttgattttca tcgccacttt atccagcggt
    30601 agcgtgccgt cggcagccgc gtcgatgggt tgcggttgaa tactgcccag caccgctgcg
    30661 ccaccggctt caaacagata attatgcgcg gcctgaccga caatatactc ttcgccgcgt
    30721 tcgcagtgac tgagcagagc gaccaggtta gcctgggtgc cggtcggcag aaaaatggcg
    30781 gcttctttac cggaaagctc tgcggcgtag tcctgcagag cattaacggt agggtcgtct
    30841 ccgtaaacgt cgtccccaac cggggcggcc atcattgctt cgagcatggc gcggctcggt
    30901 cgggtaacgg tatcactgcg taaatcaatc atggcatgtc cttattatga cgggaaatgc
    30961 cacccttttt accttagcca gttcgttttc gccagttcga tcacttcatc gccgcgtccg
    31021 ctgatgattg cgcgcaacat atacaagcta aatcctttgg cctgttcgag tttgatctgc
    31081 ggtggaatgg ctaactcttc tttggcgacc accacatcca ccagcaccgg accgtcgatg
    31141 gagaaggcgc gttgcagggc ttcatcaact tcagacgctt tttctacacg gatacccgta
    31201 atgccgcacg cttcggcaat gcgggcaaag tttgtgtcgt gtagttcggt gccgtcagtc
    31261 aaatagccac cagctttcat ctccatcgcc acaaagccca gcacgctgtt gttaaagacg
    31321 acaattttca ctggcagttt catctgcact actgagagga aatcgcccat caacatgcta
    31381 aaaccgccat cgccgcacat ggcgaccacc tgacgttctg gctctgtcgc ctgcgcaccc
    31441 agcgcctgcg gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa caggcgacgc
    31501 ttgccgttca tttttagata acgtgccgcc cacaccgttg gcgtaccaac gtcacaggtg
    31561 aaaatagcgt catcggcggc aaaatgacta atttgctgcg ccagatattg cgggtgaatg
    31621 gctttctcgc tcggtttagc taaatcgtcc agccctttgc gggcgtcgcg gtaatcttcc
    31681 agcgctttat ccagaaactt gcgatcggct ttttcttcca ccaatggaag caatgcacgc
    31741 agagtcgact tgatatcgcc gaccagtgcc atatccacct tgctgtgagc gccgatgctg
    31801 gctgggttga tatcaatctg aatgattttg gcatcggtcg ggtagaaggc gcggtaggga
    31861 aattgcgtgc cgagtagcac taacgtgtcg gcgttcatca tggtatggaa acctgacgag
    31921 aagccgatta acccggtcat tccaacatca tacggattat cgtattcgac atgttcttta
    31981 ccgcgcaggg catgaacaat aggcgcttta attttcccgg caaactcaac taactcttta
    32041 tgcgcccccg cgcagccgct gccacacatc agggcgatat tgctggaata acgcagcagt
    32101 tgcgccagtt tgcgtaactc ttcttcttcc ggcgtcacga ctggttgtgg cgcatgatac
    32161 cagtgcgtgg ttgccccttc tggcgcaggt tttaacgcca cgtcgcctgg taacacgaca
    32221 accgaaacgc cacggttaag caccgctttg cgcatggcaa tcgccagtac ttgtgggatc
    32281 tgctccgggc tggaaaccag ctcgcaatag tgactacatt cgcggaatag ctcttgtggg
    32341 tgggtttcct ggaaatagcc gctgccaatt tcgctggagg gaatatgagc ggcaatcgcc
    32401 agtaccggaa cgtgattgcg gtggcaatcg aacaggccgt tgattaagtg caggttgccg
    32461 gggccgcacg atccggcgca gaccgccagt tctccgctaa gttgtgcttc agcgccagcg
    32521 gcaaaggccg ccacttcttc gtggcgggtg gacatccact cgatggtgcc catgcgatta
    32581 agactgtcac taagaccgtt cagagagtcg cctgtgactc cccagatgcg tttcacccct
    32641 gccgattcga gtgttttggc gatataagct gcaaccgttt gtttcatggt tctccatctc
    32701 ctgaatgtga taacggtaac aagtttagtt catctgacgg agggggaagg gatgggagag
    32761 aaaggaggca ctaacggtta aatagcccga tgaaaggaat atcatcgggc ataaggcgat
    32821 tatgcgagaa ccaaatcccc ctgcggatgg caggagcagg ccagtacgta accttcagcg
    32881 atttcggcgt cggtcagcgt cattgtactg ctcaccgtat attcaccgga aaccactttt
    32941 gtcttacagc agccgcaaac acccgcacgg caggcagcga caaccggaac gttattgctt
    33001 tccagcgcct ccagtagcgt ggtgccaacc ggggcgtaaa attctcgtgc cggttgcagt
    33061 ttggtgaatt tcagaccgct ggtcgccgct tctgctactg gagtgaagaa tttctcttta
    33121 aagaaacgcg tcacgccgag cgctttcact tcctgctcta cccaatccat atacggagcc
    33181 gggccgcagg tcatcacggt acgtgaagct aagtcaggta cacctgccag cagttcgcga
    33241 gtgagacgac cagcgataaa gccttcggta acgttatttt ctgccaccag cgttaccgga
    33301 tagttacgcc actcatcggc gaaaataaca tcctgcggcg tacgcacgtt gtagatcacc
    33361 cgcacatcgg cctgtggacg gttcttcgca agccagcgac gcatcgacat aatcggcgtg
    33421 acgccgcagc ctgccgccag caacaggaat ttatcttctg ctttatcgtc gcaggtaaat
    33481 tcccccatcg cgtccgaaag ccagagataa tcaccgcgtt ttacatcgcg cgtcagccac
    33541 tgggagccga caccgtcatc aatccgccgc acggtcaggg tgatatattc actcacgcct
    33601 ggcgtggagg aaatggtgta agcacgcagc gtttccgctg agtttcgcac gctgaccagt
    33661 gcatattgcc cggcgcgata tgggtagtaa tcgtggcaaa tcagggaaat cgtccacaca
    33721 tccggcgttt cttgcgtaat gtgatgaacc tgcatccgcc acgggcattg attcgttggc
    33781 atcgtcatcg acaaactcct tacgcgctca acagttgctt catgtcttct tcaacagtgg
    33841 tgatagaacg caggccgaat ttctcgttca gcaccgccag caggtctggt gtcaggaaac
    33901 caggtgcagt cgggccggtg acgatatttt tcacgcccag agaaagcagc gtcagcagaa
    33961 tgacgatcgc tttctgttca aaccaggaga gcaccagcga cagcggcaga tcgttcacac
    34021 cacagcccag tttctctgcc agagtgacag ccagaataat cgctgagtaa gcatcgttac
    34081 attgacctgc atctaccaga cgcggcagac cttcgatatc gccaaactca agtttgttaa
    34141 agcgatattt accacaggcg agggtcagga tcaggcagtc atccggcacg ctggtggcga
    34201 aatcggtgaa gtagtggcgc tcgccgcgtg cgccgtcaca gccaccaagc aggaagatat
    34261 gacgcagttt ttcacggctc accagatcaa tcagcgtatc agcagcgcca agcagcgtct
    34321 ggcgaccaaa acccacggtg ataaggtgcg gaatttcgct gtacgggaag cctgccatct
    34381 gttgcgcctg ggtgataacc gcagagaaat catcaccgtc cagatgacgc acgccaggcc
    34441 agccaacaat gctgcgggtc cagatacgat cgtcataagc gcctacggtt gggtcgatga
    34501 tgcagttcga ggtcatcacg atggggccag ggaaacgagc gaactccact tgctgattct
    34561 gccagccgct gccgtagtta ccgaccagat gcttgaattt acgcagctcc gggtagccat
    34621 gcgcaggcag catttcgccg tgggtgtaga cattaacgcc cgtgccttcg gtctgttcca
    34681 gcaggttgta gagatctttg agatcgtgac cggaaatcag aatgcattta cccgccgtcg
    34741 ctttgacgtt gacctgggtt ggcgtcgggt gaccgtattt accggtttcg cctgcatcca
    34801 gaatgctcat cactttgaag ttcatctggc cgatttccat tgaacactca agaagcgcgt
    34861 tcatatcggc aggccaggtc cccagccacg ccatgatttt atggtactgg gtataaatat
    34921 cgttgtcgta ttgaccgaga acatgcgcgt gttccatata ggccgccgca cctttcaggc
    34981 catacaggca cagcagacgc aggccgagaa tgttttcgcc aatcgccgct ttatctttgt
    35041 taggggtaaa ttctgctgcc tgacgttgca gctcgccgag atcatcgctc accagttgca
    35101 ggtcagccat cgggttatcg acgcgcgcgt tggcatctac agccaggcat tgtgctttca
    35161 gcgcctcgcg caaggcaatc gcttcacgag cgtagccgac aatacgcgga gaatcgaagt
    35221 taacgttggt cagggttgag aaaaaggcac gtggcgcgaa gctgtcaaca tcgtggttga
    35281 tgatgccgta ttcacacgct tttaccgccc aggcagaaag cccttgcagc gccgcgatga
    35341 gtaaatcctg aaggtcagaa gtttccgccg ttttaccaca catcccctgc gcgtatgagc
    35401 agccgtttcc tgccggagta cggatagttt gttcacattg cacacaaaac atgatcacac
    35461 cttttaaagt tatatttaat atacatgttt aaggttaaga cgcttaacgc ggggataaaa
    35521 gggatttttc atgcaacttt aagggagatt gatttagcgc aattttggcg gcagggatct
    35581 accgccagag aggtattacg cagagaaaaa ggcgatgagg atcggcacta acaggctaag
    35641 aataaaaccg tgaacaattg ccgccgggac catatccagc ccgccagtac gttgaagaac
    35701 gggcagggtg aaatccattg atgtggcacc gcataagccc agtgcagtag agcggctgcg
    35761 gcgaatcagc ccagggatca acataatagc aatcagttca cgggccagat cattaaaaaa
    35821 cgccgcgctc ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg aaagagaata
    35881 ccagccgaaa ccggaggcca ttgccagcgc ggtattgatg gggagatcaa gaataaaggc
    35941 gttaattaaa ccaccaatta atgaactgac aaccaccacc acggcgacaa tcattccccg
    36001 gcgattaagg acaatctgct ttaaggtcat gccattattg cgcaactgaa taccaacgag
    36061 gaaaagtagc aaaattaacg tgtattcact ggcttcggtc gcgtgttgta agaaagccag
    36121 tccactcaga ccaatggcaa aaccaatcac tactacgccg cacagtttta gcgactccag
    36181 cgccatcgca atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc acggcaggcc
    36241 tcgctccagc cacatcaggg cggcaatatt acacagtaaa ataacggtaa tactgacggc
    36301 agaataatgc agaatcgcca acaggttact ggcgaggtta tcgagaaacg ccagactgat
    36361 acccataaaa aagagaataa ggtaaaccat ccagcttaat agctgattaa taacttttaa
    36421 cgcagcttgt tggcgaagcg gaatgaggta acctacaatc aggggaacca gaatgattaa
    36481 cagcccagaa aacatgaaaa cccagtcctt gcaaagatga agtcgaaatg cgcgatgaca
    36541 cactactgaa agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt atgcataagg
    36601 agcagtagag tattcgtttt catttaaaga tattcttgcg ctttaattac aaactgcacc
    36661 gatgttggtg gcgtcaaaat cgccgaggcg ttccctgaag gccggggcag
//
LOCUS       DAJTYZ010000029        36012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000029 DAJTYZ010000000
VERSION     DAJTYZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36012
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.029"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            407..1855
                     /gene="uacT"
                     /locus_tag="OO596_001379"
     CDS             407..1855
                     /gene="uacT"
                     /locus_tag="OO596_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6717279.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1857..1982
                     /locus_tag="OO596_001380"
     CDS             1857..1982
                     /locus_tag="OO596_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717280.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1979..2050)
                     /locus_tag="OO596_001381"
     CDS             complement(1979..2050)
                     /locus_tag="OO596_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717281.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2105..2653
                     /gene="idi"
                     /locus_tag="OO596_001382"
     CDS             2105..2653
                     /gene="idi"
                     /locus_tag="OO596_001382"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6717282.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2696..4213)
                     /gene="lysS"
                     /locus_tag="OO596_001383"
     CDS             complement(2696..4213)
                     /gene="lysS"
                     /locus_tag="OO596_001383"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6717283.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4223..5321)
                     /gene="prfB"
                     /locus_tag="OO596_001384"
     CDS             complement(join(4223..5245,5247..5321))
                     /gene="prfB"
                     /locus_tag="OO596_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6717284.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5412..7145)
                     /gene="recJ"
                     /locus_tag="OO596_001385"
     CDS             complement(5412..7145)
                     /gene="recJ"
                     /locus_tag="OO596_001385"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6717285.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPVAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7151..7861)
                     /gene="dsbC"
                     /locus_tag="OO596_001386"
     CDS             complement(7151..7861)
                     /gene="dsbC"
                     /locus_tag="OO596_001386"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6717286.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7886..8782)
                     /gene="xerD"
                     /locus_tag="OO596_001387"
     CDS             complement(7886..8782)
                     /gene="xerD"
                     /locus_tag="OO596_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6717287.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8894..9415
                     /gene="fldB"
                     /locus_tag="OO596_001388"
     CDS             8894..9415
                     /gene="fldB"
                     /locus_tag="OO596_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6717288.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9455..9862)
                     /locus_tag="OO596_001389"
     CDS             complement(9455..9862)
                     /locus_tag="OO596_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR6717289.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9843..10109)
                     /gene="sdhE"
                     /locus_tag="OO596_001390"
     CDS             complement(9843..10109)
                     /gene="sdhE"
                     /locus_tag="OO596_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6717290.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10352..11332
                     /gene="ygfZ"
                     /locus_tag="OO596_001391"
     CDS             10352..11332
                     /gene="ygfZ"
                     /locus_tag="OO596_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6717291.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11528..12187)
                     /locus_tag="OO596_001392"
     CDS             complement(11528..12187)
                     /locus_tag="OO596_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6717292.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12351..12662)
                     /locus_tag="OO596_001393"
     CDS             complement(12351..12662)
                     /locus_tag="OO596_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR6717293.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12707..14140
                     /gene="bglA"
                     /locus_tag="OO596_001394"
     CDS             12707..14140
                     /gene="bglA"
                     /locus_tag="OO596_001394"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6717294.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14197..14940)
                     /locus_tag="OO596_001395"
     CDS             complement(14197..14940)
                     /locus_tag="OO596_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6717295.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15206..18079)
                     /gene="gcvP"
                     /locus_tag="OO596_001396"
     CDS             complement(15206..18079)
                     /gene="gcvP"
                     /locus_tag="OO596_001396"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6717296.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCSMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18198..18587)
                     /gene="gcvH"
                     /locus_tag="OO596_001397"
     CDS             complement(18198..18587)
                     /gene="gcvH"
                     /locus_tag="OO596_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6717297.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18611..19705)
                     /gene="gcvT"
                     /locus_tag="OO596_001398"
     CDS             complement(18611..19705)
                     /gene="gcvT"
                     /locus_tag="OO596_001398"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6717298.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            19995..20078
                     /locus_tag="OO596_001399"
     CDS             19995..20078
                     /locus_tag="OO596_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717299.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20152..21354)
                     /gene="ubiI"
                     /locus_tag="OO596_001400"
                     /gene_synonym="visC"
     CDS             complement(20152..21354)
                     /gene="ubiI"
                     /locus_tag="OO596_001400"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6717300.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21377..22555)
                     /gene="ubiH"
                     /locus_tag="OO596_001401"
                     /gene_synonym="visB"
     CDS             complement(21377..22555)
                     /gene="ubiH"
                     /locus_tag="OO596_001401"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6717301.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22552..23877)
                     /gene="pepP"
                     /locus_tag="OO596_001402"
     CDS             complement(22552..23877)
                     /gene="pepP"
                     /locus_tag="OO596_001402"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6717302.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23903..24481)
                     /gene="ygfB"
                     /locus_tag="OO596_001403"
     CDS             complement(23903..24481)
                     /gene="ygfB"
                     /locus_tag="OO596_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6717303.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24649..24978
                     /gene="zapA"
                     /locus_tag="OO596_001404"
     CDS             24649..24978
                     /gene="zapA"
                     /locus_tag="OO596_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6717304.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25020..25203
                     /gene="ssrS"
                     /locus_tag="OO596_001405"
     ncRNA           25020..25203
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO596_001405"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25224..25826
                     /locus_tag="OO596_001406"
     CDS             25224..25826
                     /locus_tag="OO596_001406"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6717305.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25927..25986)
                     /locus_tag="OO596_001407"
     CDS             complement(25927..25986)
                     /locus_tag="OO596_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6717306.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26318..26377)
                     /gene="ibsC"
                     /locus_tag="OO596_001408"
     CDS             complement(26318..26377)
                     /gene="ibsC"
                     /locus_tag="OO596_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6717307.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26606..27838)
                     /gene="serA"
                     /locus_tag="OO596_001409"
     CDS             complement(26606..27838)
                     /gene="serA"
                     /locus_tag="OO596_001409"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6717308.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28094..28753)
                     /gene="rpiA"
                     /locus_tag="OO596_001410"
     CDS             complement(28094..28753)
                     /gene="rpiA"
                     /locus_tag="OO596_001410"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6717309.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29183..30076
                     /gene="argP"
                     /locus_tag="OO596_001411"
     CDS             29183..30076
                     /gene="argP"
                     /locus_tag="OO596_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6717310.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30279..32423
                     /gene="scpA"
                     /locus_tag="OO596_001412"
     CDS             30279..32423
                     /gene="scpA"
                     /locus_tag="OO596_001412"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6717311.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32416..33411
                     /gene="meaB"
                     /locus_tag="OO596_001413"
     CDS             32416..33411
                     /gene="meaB"
                     /locus_tag="OO596_001413"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6717312.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33422..34207
                     /gene="scpB"
                     /locus_tag="OO596_001414"
     CDS             33422..34207
                     /gene="scpB"
                     /locus_tag="OO596_001414"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6717313.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34231..35709
                     /gene="scpC"
                     /locus_tag="OO596_001415"
     CDS             34231..35709
                     /gene="scpC"
                     /locus_tag="OO596_001415"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR6717314.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35706..>36012)
                     /locus_tag="OO596_001416"
     CDS             complement(35706..>36012)
                     /locus_tag="OO596_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6717315.1"
                     /translation="FQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFL
                     TVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8615 a         9670 c         9098 g         8629 t
ORIGIN      
        1 cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac cgttttaatt
       61 ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt cattatttca
      121 acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag agtgcgacat
      181 gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca ttgtcaggtt
      241 ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat tcctgtctct
      301 aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca gtttcattaa
      361 cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga gcgccataga
      421 ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg accgcatatt
      481 atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt acgcaggtgc
      541 agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg ctattgcgat
      601 gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat gtatcggtat
      661 cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg ctgctgtaac
      721 accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat ttggtgccac
      781 tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct tgatgccttt
      841 attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca tcattcaggt
      901 gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc ccgtttattt
      961 aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg cgaaaggatt
     1021 tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt cgtggatgat
     1081 gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg tcacgccgat
     1141 gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg ccgtgttaat
     1201 catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag tcggtcgtaa
     1261 actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga caatgatagg
     1321 cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc tggttagcgt
     1381 gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa tcctgtttgg
     1441 catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc tgggcggtgc
     1501 tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt cgcgctgtaa
     1561 ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg ttggcatgac
     1621 tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac cgttgctgca
     1681 tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct ttaatggcta
     1741 ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa aagtcaggac
     1801 agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag aataatatga
     1861 attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct attcctgtca
     1921 tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg ctactttttt
     1981 aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa acgctcactt
     2041 ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt acatgtgaga
     2101 aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca cgggtacgct
     2161 ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct ccagttggct
     2221 gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa aagcgtggcc
     2281 tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca acgaagacgc
     2341 ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg aatctatcta
     2401 tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg aagtgtgtcc
     2461 ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag tgatggatta
     2521 tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt gggcgttcag
     2581 tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt ctgctttcgc
     2641 gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt aatgcttatt
     2701 ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg ttggtgaaca
     2761 gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag ccatgttcca
     2821 gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg tctttcgcgg
     2881 caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg ctaaagccgt
     2941 taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc gggttaatgt
     3001 cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag gtcggctgaa
     3061 tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc agaccccagc
     3121 tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg aagttatcca
     3181 gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg gtcagttttt
     3241 cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc ttaccgagaa
     3301 tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct ttgtaatctg
     3361 cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg gaaatacctt
     3421 cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc accagacgct
     3481 tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg ttatggtggg
     3541 tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc gtttcaactt
     3601 ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc gagcgtactt
     3661 taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga cgatagcgcg
     3721 cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct ttggtcagca
     3781 gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac agtttacctt
     3841 tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa acgccttccg
     3901 gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc agggtaacga
     3961 aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg acttcgatgt
     4021 tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat tggtcagagg
     4081 tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg ttcgccagct
     4141 tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg ccctgtgcgt
     4201 gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg ataaattgat
     4261 ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta cgcagatctt
     4321 taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg atgtcagatt
     4381 tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt tcataaagct
     4441 tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg tcgttctggc
     4501 actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg gtacggttaa
     4561 cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc gccgggttga
     4621 tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg aacgacgtgt
     4681 ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg ccagtttctg
     4741 tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat ttaatacccg
     4801 ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat tctgcccagc
     4861 gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg ccagaccccg
     4921 cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata cggcggaact
     4981 caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg ttaaaggttt
     5041 cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc agcccctgtt
     5101 tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta cccagcgcct
     5161 gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt acttcttcca
     5221 gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga gcgttccgtg
     5281 aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt tcttttattg
     5341 agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc ttttttatag
     5401 agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct gcggttgccg
     5461 cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc gttatccggc
     5521 cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc gccgaccggt
     5581 tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag ccggaaatga
     5641 ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg cagcagctgc
     5701 gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac ttcgccttgc
     5761 aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg aaagagttcg
     5821 aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa cttcagcatc
     5881 atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag cccctgaatg
     5941 gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat aaccggacgg
     6001 tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg ccattcgggg
     6061 tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt ctcgcacagg
     6121 gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt tagcgcatcg
     6181 agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag cgccacaccg
     6241 acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc aaaacctaaa
     6301 tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc tttaatcccc
     6361 ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat gcgattatta
     6421 gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag cagttctgcc
     6481 aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa aaaggtgcgc
     6541 agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga cgggaaatta
     6601 cagtcgcgca agttagggtt aatgatcgct tccgccacgg gtaatgtttc gcccggcaaa
     6661 tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac ccccgcatgg
     6721 gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg ggcctgatcg
     6781 accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag gtagtcgata
     6841 ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt ggcaccgtcg
     6901 gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt gtaaaggatc
     6961 tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt aacactgcgt
     7021 tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag caagggaggc
     7081 aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg tatctgttgt
     7141 ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat tctttcatct
     7201 ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact gccggagtac
     7261 cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg tcgcagctgg
     7321 ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta tctttcgcac
     7381 accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc gggaaagcaa
     7441 gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc agtttgtggc
     7501 agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc agcgctttat
     7561 aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta ttggtgacat
     7621 tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt ttaccatcat
     7681 cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct acgggcgcgg
     7741 gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt gccgcgtcat
     7801 cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa cctttcttca
     7861 taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga tgaagttgcc
     7921 gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg ctgtggccca
     7981 gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg gcaaaagcgt
     8041 gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg gcataatgtt
     8101 taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg ggaaacaaca
     8161 cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag gtttccagcc
     8221 agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta ccaatgaccc
     8281 gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt tcagagacac
     8341 gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc agtggctgat
     8401 caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct tttggcaaac
     8461 gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga aacttttcgc
     8521 gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag ctggtggctt
     8581 tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt tgcgccgtcg
     8641 ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg cgacggtaag
     8701 cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga aactgctcga
     8761 tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat ccattatgcc
     8821 ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac agcagaatcg
     8881 agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt acaccgaaat
     8941 ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac ataacctcaa
     9001 ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta tcccgacctg
     9061 ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg acgacctgaa
     9121 ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat acggcgagtg
     9181 gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg tgaagttcgt
     9241 cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga ttgctgacgg
     9301 gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta gcgacgagcg
     9361 tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg cctgatctca
     9421 ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct tgttgcaaca
     9481 aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc agccataaat
     9541 gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta atcatccacg
     9601 gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc atcaacaagc
     9661 gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca aacaccacca
     9721 gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg agtaaaataa
     9781 cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag gagacgcgca
     9841 aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg gaccatcatt
     9901 tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag gtccggatcg
     9961 tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct gtcgtactca
    10021 tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg gcatgcccaa
    10081 tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa tccagtaccc
    10141 cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca ccatcgcgat
    10201 cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc ctcgcagaag
    10261 gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc attagtcatt
    10321 aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc ctccccgtca
    10381 gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact gggcgcttgc
    10441 caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag cagatgtcag
    10501 ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag gcaaaatgtg
    10561 gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac ggcgcagcgt
    10621 gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag tgaccatcgc
    10681 gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg ccgcgctggc
    10741 aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag gcgcgaccac
    10801 tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg aagctactgc
    10861 taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc aacagtggct
    10921 ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg ggcagtttat
    10981 cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag gctgttatac
    11041 cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg cgctctggtt
    11101 gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc tgaaaatggg
    11161 cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag atggtcaggt
    11221 cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc gcgtacgcga
    11281 cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt aaatctcttt
    11341 atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg tctgggattt
    11401 gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc acgccgaatc
    11461 cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc ctacaaaggc
    11521 atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac acactgccgc
    11581 cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag acatagaaaa
    11641 tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg cccgccgcga
    11701 gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc agataggtca
    11761 ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata cccagcaatg
    11821 ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc cccaccagca
    11881 aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca aatttcttca
    11941 gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg aggaacagca
    12001 ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg gcattgagat
    12061 ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac ccaatgccgt
    12121 gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg aggggcttct
    12181 gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca gcgtaactga
    12241 gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac taatctccta
    12301 aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat ttaaagacat
    12361 ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat gacctttatc
    12421 agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt atccagcgtt
    12481 acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc aaaacgcccg
    12541 acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat ggtgatggtt
    12601 ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc gtttggctgc
    12661 atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga aaaaactcac
    12721 cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg aaggcggctg
    12781 gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg cacacggcgt
    12841 gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc atgaagccgt
    12901 tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg gcttcaaatg
    12961 ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag ctcagccaaa
    13021 cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat acaacatcga
    13081 accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc aatacggtag
    13141 ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg tgtttgaacg
    13201 ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc agcgtaactg
    13261 gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc atgagaaccc
    13321 ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc tggcggtgaa
    13381 agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga tggtgccgct
    13441 ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga tgcgcgaacg
    13501 ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt tgaacgagtg
    13561 ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac tgcgcgaagg
    13621 cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg ccgaaggcgg
    13681 caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg ttaaagcgtc
    13741 tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg aactgtatga
    13801 gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg acaaagtgga
    13861 agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc atatcgaaga
    13921 gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac cgtggggctg
    13981 catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct ttatctatgt
    14041 gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga gctttaactg
    14101 gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag taccggatgc
    14161 aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg ccaaatcgat
    14221 aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg cctgcgcgac
    14281 ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat cgacgcgtcc
    14341 aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca cgcagttaac
    14401 gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat caatcgcccc
    14461 tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg ccaccgaaaa
    14521 gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt ttaccgcctc
    14581 gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga ttcgctctgc
    14641 ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga ccagcgctgc
    14701 tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat tttcgtcgct
    14761 gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt tcaccacttc
    14821 ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt ctttcgccaa
    14881 cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg ctatagccat
    14941 ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa caaactggct
    15001 caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc cggggtgagg
    15061 gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc ccaaaggggc
    15121 gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg ccgaagcgcc
    15181 tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg caggagcaga
    15241 acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag tatttgtctg
    15301 ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac tcggcgacca
    15361 gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc cagacagcgg
    15421 ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag cgatccagtt
    15481 ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc gggaacgaca
    15541 tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc tcgctgatgc
    15601 cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg cgaccgtcgc
    15661 gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag ttggcgttga
    15721 ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg atgtacatcc
    15781 agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg ccctgacggg
    15841 ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc agatgcgctt
    15901 tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag aaagttttat
    15961 gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg ccaacctggg
    16021 cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg acttcacaca
    16081 cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc atgatacagg
    16141 agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg atgttgccgt
    16201 ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa gcggggttag
    16261 ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg cggctttcat
    16321 gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag ttcggctgca
    16381 tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc gcaatcatct
    16441 gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca aattccggcc
    16501 aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggagcaggaa cccagcggga
    16561 tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag cgcatcattt
    16621 cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg tcgcgcagca
    16681 tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg tcgatctcca
    16741 gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca cgcgtggtgg
    16801 tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg atttcagccg
    16861 cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag gtgtcgaaat
    16921 agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga atatcggtca
    16981 gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa acggcataca
    17041 ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc gctttctcac
    17101 gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg ccagctgcat
    17161 ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct ttcgccgcaa
    17221 agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc gccgaaccaa
    17281 aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc ataatatcgg
    17341 cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca gtgtagtcgt
    17401 gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc tgatggtcga
    17461 gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca cgagtacgga
    17521 ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg gcatttttca
    17581 gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc tcgtccagaa
    17641 gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg aagttgagca
    17701 gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg gtataccagc
    17761 ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg gtgtaaccca
    17821 tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt tctgccagtg
    17881 cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga atatctttcg
    17941 gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc agcatttctt
    18001 gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg ttttcaagct
    18061 ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt gtgaacctct
    18121 ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc ctctcccgca
    18181 gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt cgcatccagc
    18241 agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc acctgcatac
    18301 ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc cacgatttca
    18361 ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac cgcgcagtca
    18421 tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat atcgcctaac
    18481 agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc tttacgcagc
    18541 cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa tcaatctcca
    18601 aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt gtcactttaa
    18661 ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct tccagcacgc
    18721 gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg ataatgcctt
    18781 catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc agcacgcctt
    18841 tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc acttccaggg
    18901 cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag cccatgttgg
    18961 cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg cccgcttcca
    19021 gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc accagcgcac
    19081 gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct tcaccggtat
    19141 aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt ttcatccctt
    19201 ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc gcattcggcc
    19261 cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg aaaggttcag
    19321 cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca acgaggcgga
    19381 agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca gaggcattca
    19441 acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg ttcgccagca
    19501 gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc atatgtgaca
    19561 catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc gaaccgtaat
    19621 gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc gtgtgttgtt
    19681 cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc tgacaacttt
    19741 tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc cccaacgcaa
    19801 tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag gtaaaattga
    19861 tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa aaaccaaaaa
    19921 acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat aaggcaatat
    19981 taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt agaaaatata
    20041 atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc ggcgaaaaaa
    20101 ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt tttaacgcag
    20161 ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt taacgccagg
    20221 aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg gattggtacc
    20281 ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac tgtgcttgcg
    20341 gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct gacgatgcaa
    20401 ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta ctccctgccc
    20461 cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt gcgcggcaaa
    20521 ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa ccttgcataa
    20581 gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt cacttgcctg
    20641 ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa gatgcggatc
    20701 gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg ccaccgcatc
    20761 atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat cccagaaagt
    20821 cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc caatcaccag
    20881 acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt ctccccaggc
    20941 gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct gcgctttgtt
    21001 ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc tatagcccat
    21061 gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt ccataccgtg
    21121 ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg tgagtaattt
    21181 ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg ccgccagagg
    21241 ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta agccgcaggc
    21301 aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt gcattgttta
    21361 ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc agcacatcgc
    21421 gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca accagcggtg
    21481 cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt gcttcgcgat
    21541 cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg tcttctccgc
    21601 gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata ccgaggttaa
    21661 acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc acggtacgat
    21721 gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt ttaccggcgt
    21781 gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa aacttctcgt
    21841 cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc agcgaacagc
    21901 gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt tcaaaagcgc
    21961 ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt tcgtaaggct
    22021 cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca tcagctgcta
    22081 ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg tgactctgag
    22141 tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt gctttacgca
    22201 gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc agcgccgcca
    22261 gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg ctgacatgca
    22321 cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg cgcgccagtt
    22381 gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga tgagcatgtg
    22441 actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt aaccgggaaa
    22501 tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg ctcattgctt
    22561 tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc tggcggtgag
    22621 gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc cgataccgcg
    22681 atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta cggtcagtac
    22741 catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt catggacatc
    22801 cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt tctgagcgat
    22861 cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa ccatgatgcg
    22921 caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc gcaggctggt
    22981 ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga atttgccgtt
    23041 gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac ccgcgtcaat
    23101 caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca gaatgcagcc
    23161 gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt ggcggttaaa
    23221 ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc atttttccat
    23281 cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg caatctcttc
    23341 tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca tcgttgccgg
    23401 tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt tcatgatttc
    23461 atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt taagtagttg
    23521 ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca gtttctctgg
    23581 cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt cgcgaacgcg
    23641 gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca gcaccgcttc
    23701 caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg ggtattcgct
    23761 gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc cgggttgcat
    23821 ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct cactcataac
    23881 actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct ggcgcggtcg
    23941 gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg tattcgatga
    24001 tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa cccagttgcg
    24061 caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta tccagcttcg
    24121 gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat gcatcagccc
    24181 gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg aagccgtcat
    24241 cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc agctcatgac
    24301 cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag ctgtcatcgt
    24361 taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc agccccgtcc
    24421 cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc tgtatagaca
    24481 taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg ggccctggtg
    24541 gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc cttcgcagac
    24601 catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat gtctgcacaa
    24661 cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc tgaccaaagg
    24721 gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct gaaagaacgc
    24781 actagagtca caaatactga acagttggtc ttcattgccg cattgaatat cagctatgag
    24841 ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca gcgtattcgg
    24901 atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac cgaaaaaact
    24961 aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt gaagacaaaa
    25021 tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat accacaagaa
    25081 tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta aaactgcgac
    25141 gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca tctcggagat
    25201 tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg acattatccc
    25261 gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg gaacaacagc
    25321 aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg gtgatggcgc
    25381 atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg ctaatagaac
    25441 aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt agtgccggta
    25501 atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg ttgaagatcc
    25561 atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac gtgctgatca
    25621 caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt ggtttttatg
    25681 atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat gcgcatgatt
    25741 gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct gcggtggtta
    25801 caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg attgacatcg
    25861 ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct cccctgtgac
    25921 tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga tggcaagtcg
    25981 catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt tacagcccca
    26041 tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc tacctttaaa
    26101 tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt tgggaaaaag
    26161 gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc ggttcagggc
    26221 gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt tagtgagtaa
    26281 gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg aaacttatca
    26341 gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt taagccctcg
    26401 cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa atccccatta
    26461 aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact cgcaaagtag
    26521 aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt cagtgacctg
    26581 cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg gtacccggaa
    26641 tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca atatcaataa
    26701 ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg acgccctgct
    26761 cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg atgtgcatca
    26821 gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca gagagcgttg
    26881 agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata ttctcctgcg
    26941 cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat tcacacagtg
    27001 gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg attgccgccc
    27061 ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc acagtaccgc
    27121 gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc gcgcccatca
    27181 tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc atattcagca
    27241 ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt tcaatatcat
    27301 aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta ccaatatgac
    27361 cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc gccagtttgt
    27421 tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat agcagcagca
    27481 gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg ttaaataccg
    27541 ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata cagaaacagc
    27601 caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg gtacgggatc
    27661 gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca tccagcgcgc
    27721 ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc gccttttggt
    27781 gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc tttgccattt
    27841 acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc aacatatcaa
    27901 aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat atcacgcaaa
    27961 ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc gacaatagcc
    28021 aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta acgggggagg
    28081 ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca atcagcgcaa
    28141 cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc gcatttatgg
    28201 cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg atcacgttgc
    28261 cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc accagctggc
    28321 gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat ttgcccagaa
    28381 tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc gaagcaatga
    28441 ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg ccgttgattt
    28501 catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca aaaacgtgaa
    28561 tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc ccttcaatct
    28621 ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct gtacctacac
    28681 caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct tttttcaatt
    28741 catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt atatacaaaa
    28801 aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa atatcgcgca
    28861 atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg tatgccgctc
    28921 cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta caggcaaatt
    28981 gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga gcgaaatgca
    29041 ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa atgttacccg
    29101 ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag gaaaaagcat
    29161 ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac aggcactgga
    29221 tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca ttacacaatc
    29281 agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc tgttggtgcg
    29341 taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc tgcgccaggt
    29401 ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc cgctgctgct
    29461 ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac tggctcctgt
    29521 gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc gtactcagga
    29581 acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg cgctgccgag
    29641 ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa aaccctttgc
    29701 cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac ctgtggtcgc
    29761 ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg atctgcctcc
    29821 aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac ttgctcgcca
    29881 gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag ccagcggtga
    29941 actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc accgctttgc
    30001 tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg gtcacaaagt
    30061 ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc gttatcctca
    30121 cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc ggcaaaaaac
    30181 gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca ccgtaagcgc
    30241 aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag caggagtggc
    30301 aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg ctggttcagc
    30361 aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc gataatctgg
    30421 aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt gccactatgt
    30481 ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca aaagagtcca
    30541 acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt gcgtttgacc
    30601 ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac gtcggcaaag
    30661 cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag atcccgctgg
    30721 ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg gcgttttata
    30781 tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc attcaaaacg
    30841 atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa ccgtcaatgc
    30901 gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt aataccatca
    30961 gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag gtagcattta
    31021 cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg aaaattgatg
    31081 acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt atgaacgtcg
    31141 ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt ggcgcacagg
    31201 atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc ctgactgaac
    31261 aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg acgctgggcg
    31321 gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct accgatttct
    31381 cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa ctctgccgca
    31441 ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa atcgtcaaac
    31501 aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa gcgatcgaag
    31561 caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg ctgatcgacc
    31621 aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa gacgaaaccg
    31681 atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg ctggaacgca
    31741 ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg actcacgccg
    31801 cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt cgcgccaccc
    31861 tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg ccaagccagt
    31921 gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc tccgagttcg
    31981 atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc ccgcgcattc
    32041 tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc gccagcgcct
    32101 attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct gaagagatcg
    32161 cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg gctgccgatc
    32221 ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc gaagatatct
    32281 gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa gagcgcggcg
    32341 tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac gtactgaatc
    32401 tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg ccgcttacgt
    32461 cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg tcacccacgt
    32521 catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg taacgccctg
    32581 cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga agcctttggc
    32641 atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc cagcagcccg
    32701 gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc gcgtgccgag
    32761 gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag tcagcgagca
    32821 cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt cgaaacggtt
    32881 ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat ctcgttgcaa
    32941 attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga agtggctgat
    33001 ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat tgcccggcat
    33061 atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca gccacgggtt
    33121 ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc catcatcgac
    33181 ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca acaatcggtg
    33241 gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc gaatgaagat
    33301 ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct ctcaccgcgc
    33361 accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta aaggaatttt
    33421 tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca ttgagtttaa
    33481 ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta tgcaggcgtt
    33541 aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa gtggatccaa
    33601 agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg atccgctctc
    33661 ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga aaccgatcat
    33721 ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga gttccgatct
    33781 gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg gcgtcccgta
    33841 taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg tcaaagagct
    33901 gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca tcctcaacca
    33961 tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc acatctctga
    34021 gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg gcgaagcgca
    34081 caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg tttatgacag
    34141 cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta atttcgttgg
    34201 tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag gatgaccgcc
    34261 gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg ctttacccca
    34321 gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga acagcatgag
    34381 gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc cgccgctgac
    34441 gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac atcgtccggt
    34501 ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt gagcgaagtg
    34561 gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat cgaagcatcg
    34621 gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc gccgacatgg
    34681 ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc gcgcgttgca
    34741 gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt gtcgatcttc
    34801 catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa gattgtcgcc
    34861 gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa tcccatgtgc
    34921 cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca tgggcgtatt
    34981 ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc ggtaatggcg
    35041 cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt gctacaggaa
    35101 tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc cagcctgaca
    35161 atctcggccg attccctgcg caagatttac gacaatatgg attactttgc cagccgcatt
    35221 gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct gggcgtcatc
    35281 gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac gcacgtagcc
    35341 ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa cgcgtatctg
    35401 tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt cgtgccaatg
    35461 tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga acaagggatc
    35521 gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga taattgtgca
    35581 catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg cggacatatt
    35641 caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac cggctcgatg
    35701 ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat cgtggcggta
    35761 taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct gtgcggcgca
    35821 tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg gatcaccgtc
    35881 agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg acgataaata
    35941 atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc gaagttagcc
    36001 cggttttgaa ag
//
LOCUS       DAJTYZ010000030        35061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000030 DAJTYZ010000000
VERSION     DAJTYZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.030"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..581)
                     /locus_tag="OO596_001417"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="OO596_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="OO596_001418"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="OO596_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6717316.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OO596_001419"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OO596_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6717317.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OO596_001420"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OO596_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6717318.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="OO596_001421"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="OO596_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6717319.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="OO596_001422"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="OO596_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6717320.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="OO596_001423"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="OO596_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6717321.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="OO596_001424"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="OO596_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6717322.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OO596_001425"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OO596_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6717323.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="OO596_001426"
     CDS             complement(10994..11137)
                     /locus_tag="OO596_001426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717324.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="OO596_001427"
     CDS             11138..11416
                     /locus_tag="OO596_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717325.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="OO596_001428"
     CDS             complement(11463..13559)
                     /locus_tag="OO596_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6717326.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="OO596_001429"
     CDS             complement(13559..14296)
                     /locus_tag="OO596_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6717327.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="OO596_001430"
     CDS             complement(14293..14931)
                     /locus_tag="OO596_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6717328.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="OO596_001431"
     CDS             complement(15045..15287)
                     /locus_tag="OO596_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717329.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OO596_001432"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OO596_001432"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6717330.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="OO596_001433"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="OO596_001433"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6717331.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="OO596_001434"
     CDS             19524..19796
                     /locus_tag="OO596_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6717332.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OO596_001435"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OO596_001435"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6717333.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="OO596_001436"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="OO596_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="OO596_001437"
     CDS             22056..22862
                     /locus_tag="OO596_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6717334.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="OO596_001438"
     CDS             22872..23279
                     /locus_tag="OO596_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6717335.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="OO596_001439"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="OO596_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="OO596_001440"
     CDS             23839..24636
                     /locus_tag="OO596_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6717336.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="OO596_001441"
     CDS             24647..25471
                     /locus_tag="OO596_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6717337.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="OO596_001442"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="OO596_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27823
                     /gene="pepE"
                     /locus_tag="OO596_001443"
     CDS             27134..27823
                     /gene="pepE"
                     /locus_tag="OO596_001443"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6717338.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TCDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27914..29545)
                     /locus_tag="OO596_001444"
     CDS             complement(27914..29545)
                     /locus_tag="OO596_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6717339.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OO596_001445"
     CDS             complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OO596_001445"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6717340.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33648..34472
                     /gene="iclR"
                     /locus_tag="OO596_001446"
     CDS             33648..34472
                     /gene="iclR"
                     /locus_tag="OO596_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6717341.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34789..>35061
                     /locus_tag="OO596_001447"
     CDS             34789..>35061
                     /locus_tag="OO596_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717342.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQADKKEITIINKTK"
BASE COUNT         8346 a         9092 c         8971 g         8652 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagttaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attattgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtctg tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg gggcgggaat actttccagt tgctgaaaca
    27421 gtgccgcgag cgcgggctgc tggcaccaat tactgacgtg gttaaacgtg gtgcgctgta
    27481 tattggctgg agcgcaggcg ctaaccttgc ttgcccaacc attcgtacca ccaacgatat
    27541 gccgattgtc gatccgcaag gtttcgatgc gctaaatctg ttcccgctgc aaatcaaccc
    27601 gcacttcacc aacgcgctgc cggaaggcca taaaggtgaa acccgtgagc agcgtattcg
    27661 cgaactgctg gtcgtcgcgc cagaactgac gattattggt ctaccggaag gtaactggat
    27721 cacggtgagt aaaggtcacg ctacgctggg tggcccgaac accacttatg tgtttaaggc
    27781 cggtgaagaa gcggtgccgc tggaagcggg tcaccgtttt taagtgaatt gccggatgca
    27841 tgatccggca tcttgttcat agtgcctgat gcgacgcttg acgcgtctta tcaggcctac
    27901 aggattcaca atattaatac tcgtcccgtc cttcatcttc atccggctgt tccagcacac
    27961 tgtaagccac cgaacaaaac agcgagttca gacgctgcat atctcccagt aagcctaaat
    28021 gtagcgaact ggtttcaatg ctctgcacgt tttgctgatg caggcgatcg acgtgggcgt
    28081 gggaatagcg gcgattaaga atgcgaaagc gatgcttgct gcggcgcaaa cgacgagcgc
    28141 tggtgacatc gccagagaag aacaccgaca ttgccagctt taaattactg agcaattgct
    28201 catagagcgc atccagttcc ttcaacccat caagcgaaaa tgcccgccgt gctgccagcg
    28261 atttatcagc aatttcgctg cccatgcgct cgacgatatc ggaagcctgt tcaaggttga
    28321 gcgacatttc aataatctcc gcccagcgac gcgactcttc ttctgccagt tcctcttttg
    28381 gcattcgcgc cagatacagc ttaatggcgg tgtagagaac gttgatatca tctgccagct
    28441 tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac tttattcagc ccttccatca
    28501 tctgttccat ggcgtcgcca atgcgcaggg tttcgcgcgc ggcgttcgcc agagcaagcg
    28561 tgggcgtatc cagcgcgctg acatccagat gtttaggccg tagctgggta tccagttccg
    28621 gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg gtcaacaaat ggcagcatga
    28681 ccaggcaacg tacaaggttg tagaagacgt ggaaatagat caccagttcc gcttttggca
    28741 atgacaactt ccccattgtc tctgccagca aatggacaaa cggcaggata atcaggctac
    28801 ccaccagctt aaacagcaaa ctacccagcg cgacacggcg ggctgcggca ttggcggcac
    28861 tgttgttgag catcgccagc aggccggaac cgaggttagc accaatcacc agacagagcg
    28921 ccacggggaa ggagataatg cctgcggcgg tcagagtcgc ggtcagcagt acagcagcaa
    28981 ggctggagta gctgataatg gcgaacatcg cgccaatcag cgcatccagc agaatatcgc
    29041 cggtcagcga ggcaaagatc acctgaacgc cgtttgcctg ggtgatcggc gttacggcct
    29101 gcacaatcaa ctccagcgcc agcaaaatca gcccaagacc aataccgacg cggcccagtt
    29161 gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac gccgataaaa ataagtaacg
    29221 gtgacagcca ggataagtcg aaggtgagaa tacgcgccat tagcgccgtc ccgacatctg
    29281 cacccagcac aatcaccaga gccggtgcga gggctaccag atcctgagcg acgaacgagg
    29341 tcaccagcat ggtggtggca ttactgctct gtaccagtgc ggtaacgccg atccccgcgc
    29401 aaaaggcgag cggctttttt tcgacgctcc ggctaaggac agtacgcaaa cgcgcgccga
    29461 agacgcgcat tacgccggtt cgaacaatat gagtccccca gaccagcagg gcgacggcag
    29521 aaagcaggtg aagcagcgtt aacacgagtt caggttctcc ttatcgttat atgttcctga
    29581 agcaattgaa tgaccaaagg accggaaaca cgctgactgg catctttact gtgccttttg
    29641 acattcgttt ttccggctaa gattattcca gcctgtttcc agtataagtg tttcatatta
    29701 ataaagagac agggcactca ttgagcgccc tgtttgttgt aaggaaaagt gacagatttg
    29761 tgaatcagtc cgcgtcatac cccagattcg gtgccagcca gcgctcaaca tcggagacgc
    29821 tcataccttt acggcgggca tagtcttcaa cctgatcgcg ctgaatttgc gccaccgcgt
    29881 agtacttgct gtccgggtgg ctgaaatacc aacccgaaac cgatgcacca ggccacatgg
    29941 cgaaagattc tgtgagtttc atgccagtat gtttttccac ttccagcagc tcccagatgg
    30001 tggctttttc cgtatgttcc gggcaggccg ggtagcccgg tgccggacgg atgccctggt
    30061 agttttcgcg gatcagttct tcgttgctga ggttctcgtt cggcgcatag ccccagtaga
    30121 ctttacgcac acgctcatgg agatactccg caaaggcttc ggctaaacgg tcggcaagcg
    30181 ctttcaccat gattttgttg taatcatcgt gctgcgcttc aaaggcatca gccagtgcgt
    30241 cctcttccag cccgccagtc acggcaaatg cgccgatgta atctgcttta ccagaaagct
    30301 tcggcgcaac gaagtcagcg agacagtagt tagcgaatcc cgttttttcg gtctgttgac
    30361 gcagatggtg gctgacgttg atcacatggg tgcgcgtttc gtcacggtag atttcaatgt
    30421 catcgcccac acggtttgcc gggaacaagc ccaccacgcc acgcggattc agcgttttct
    30481 cggcgcttaa tttatccagc atgtcgttgg cgtctttaaa cagccgctgc gcctcaacgc
    30541 ccaccacttc atcttccaga atgcgcggat acttcccggc cagcgaccag gtcataaaga
    30601 acggtgtcca gtcgatgtaa ttacgcagag tttcgatgct ggcttcaact tcctgcacgc
    30661 cgagacggtg cgccaccggc ggcgtgtaag cctgccagtc aaaagcgaag tcgttatcgc
    30721 gcgccgcttc cagcgtgacc ggtggtgtgc gcggtttctt gcgcccgtgc tgaatacgta
    30781 cggtttcata ctccttgcgg gtacgagcga caaaatcatc gcgctgagta tcggaaagca
    30841 gcgccgccac cacaccaacg gtgcgcgagg cattctgcac atacaccgtc gggccgctgt
    30901 agttctgctc aattttcacc gccgtgtgcg cttttgaggt cgtcgcgcca ccgatcagta
    30961 acggaatggt gaagccctga cgctccatct ctttcgccac gttaaccatc tcgtccagcg
    31021 acggcgtgat aagccccgaa agtccaatca gatcagcatt cacttcttta gcggtacgga
    31081 gaattttttc cgcaggcacc ataacgccga gatcgacaat ttcgtagttg ttacattgca
    31141 gcaccacacc aacgatattt ttaccgatgt cgtggacgtc gcccttcacg gtggcgatca
    31201 ccatcttgcc gttggttttg ccctgctctt tgctggcttc aataaacggt tcgaggtagg
    31261 ccactgcctg tttcatgacg cgcgccgatt tgaccacctg tggcaggaac attttcccct
    31321 cgccaaacag atcgccgacc acgttcatgc cgtccatcaa cgggccttca atcacttcaa
    31381 tcgggcgcgt agcctgctgg cgggcttctt cggtatcctg ctcgataaac tcagtaatgc
    31441 ctttgaccag cgagtattcc agacgtttat tcacttccca cgagcgccac tccgcctgct
    31501 gggcgttagc ggtgtcgtcg gttttgctgc cgcgatattt ctcggcaagc tccagtaaac
    31561 gttcagtgcc atcatcgcga cgattaagaa tcacatcttc caccgcgtcg cgcagttcag
    31621 cgggcaggtc gtcgtaaatc gccagttgcc cggcgttgac gatccccata tccatgccat
    31681 tgcgaatagc gtagtagagg aacaccgcgt gaatggcttc gcgcaccggt tcgttgccac
    31741 ggaatgagaa agaaacgtta gatacgccgc cggaaatcag cgcgtgcggc agttcgcgtt
    31801 tgatgtcttc gcaggcgccg ataaagtcct gcgcatagtt gttgtgctct tcaatgccgg
    31861 tggcgaccgc gaagatgttc gggtcgaaga tgatatcttc tggcgggaag ccaacctctt
    31921 cggtgaggat tttgtacgcc cgacggcaaa tctcgatttt ccgtgcgcgg gtatcggcct
    31981 gtccctgttc gtcaaaggcc attaccacca ccgctgcacc gtaacgacgc aacagtttcg
    32041 catgatggat aaaggcgtcg acgccctctt tcattgagat agagttaaca atgcctttgc
    32101 cctgaataca cttcagcccc ttttcgatga cctcccattt tgaggagtcg atcataatcg
    32161 gcacgcgagc gatatccggt tcaccggcaa tcagattgag aaaacgcacc atcgccgctt
    32221 cggcgtcgag catcccttca tccatattga tatcgataat ctgcgcgccg ttttccacct
    32281 gctggcgggc cacatccagt gcctcgctgt atttctcttc tttgatcagg cgcttgaact
    32341 tagcggaacc ggtgacgttg gtgcgttcac ccacgttcac aaacaggcta tcttcgccaa
    32401 tgttcagcgg ctccaggccg gacaaacggc aggctacggg gatttccggc agtttgcgcg
    32461 gcgctaatcc ttctactgca cgactcatcg ctgcaatatg ttgtggcgtg gtgccacagc
    32521 agccgccgac gatattgaga aagcccgctt ccgcccattc acgtatttgt tttgccatcg
    32581 tgtcggcgtc gagatcgtac tcaccaaagg cgttgggtag cccggcgttc gggtgcgcgg
    32641 tgacgtagca ttccgcaatc cgtgacagct cctgcacgta ctggcgcagt tcatcgggcc
    32701 ccagcgcaca gttcaggcca aaggtcagcg cttcggcgtg gcgcaatgag ttgtaaaatg
    32761 cttcggtggt ctgcccggag agcgtgcgcc cggaggcgtc ggtgatggtg ccagagatca
    32821 taatcggcag ctcaacgccc agcgtttcaa actccgtttt taccgcaaat accgccgctt
    32881 tggcgttaag ggtgtcgaaa acggtttcaa tcaggatcag atccgcacca ccttccacca
    32941 gcgctttggt ggactctcga taagccacca ccagcccgtc aaaagtgata ttacgaaatg
    33001 ccggatcgtt gacgtccgga gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg
    33061 caacgtagcg cggtttctcc ggcgtgcgcg cggtccactc gtcagcacag gcgcgcgcca
    33121 gtttcgccgc cgcaaagttg atttccgccg acagggattc catctggtaa tccgccatcg
    33181 caatggtcgt ggagttgaag gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg
    33241 cgttgtggat agcggcgatc acttccggtt tactgagtac cagcaggtcg ttgttgcctt
    33301 tgaggtcgca tggccagtcg gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat
    33361 agctctggat catggtgccc ataccgccgt ccagcaccag aatacgttca tttaactgcg
    33421 cacgcagttg ttccactttg ctgctcacac ttgctcccga cacgctcaac ccagatttaa
    33481 taaaaattca acaaaccata ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca
    33541 acatgagatt tgttcaacat taactcatcg gatcagttca gcaactattg cattaactaa
    33601 caataaaaat gaaaatgatt tccacgatac agaaaaagga gactgtcatg gtcgcaccca
    33661 ttcccgcgaa acgcggcaga aaacccgccg ttgccaccgc accagcgact ggacaggttc
    33721 agtctttaac gcgtggcctg aaattactgg agtggattgc cgaatccaat ggcagtgtgg
    33781 cactcacgga actggcgcaa caagccgggt tacccaattc cacgacccac cgcctgctaa
    33841 ccacgatgca acagcagggt ttcgtgcgtc aggttggcga actgggacac tgggcaatcg
    33901 gcgcacatgc ctttatggtc ggcagcagct ttctccagag ccgtaatttg ttagcgattg
    33961 ttcaccctat cctgcgcaat ctaatggaag agtctggcga aacggtcaat atggcggtgc
    34021 ttgatcaaag cgatcacgaa gcgattatta tcgatcaggt acagtgtact cacctgatgc
    34081 gtatgtccgc acctatcggc ggtaaattgc cgatgcacgc ttccggtgca ggtaaagcct
    34141 ttttagccca actgagcgaa gaacaggtga cgaagctgct gcaccgcaaa gggttacatg
    34201 cctataccca cgcaacgctg gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc
    34261 gcaaacgggg ctattcattt gacgatgagg aacatgcact ggggctacgt tgccttgcag
    34321 cgtgtatttt cgatgagcac cgtgaaccgt ttgccgcaat ttctatttcc ggaccgattt
    34381 cacgtattac cgatgaccgc gtgaccgagt ttggcgcgat ggtgattaaa gcggcgaagg
    34441 aagtgacgct ggcgtacggt ggaatgcgct gactttttct ggcgggcaga ggcaatattc
    34501 tgcccatcat acctgagtgg caatagaata agggtgtctg ttaatcgcat tgacgccaaa
    34561 ataacttaat gtcatacact tcacacactt ttattaactc agcattattt ttaaacatca
    34621 aaccacttaa ttattaacaa aatcgcaaga tctcattcca ccgaaacttt tacggctgca
    34681 ttatgagatt gagcgattta tgatatttct ttagttgtaa tcatattaaa ttttttcact
    34741 gttctgatag ttaaaattta agacatcaat aaacaatgag atatttaaat gattactcgt
    34801 attcctcgta gttctttctc tgcaaatatt aataatacag cccagacaaa tgaacaccaa
    34861 accctgagtg aattgtttta taaagaactc gaggataaat tttctggcaa ggagctggcg
    34921 acgcctctat taaaaagctt ctcagagaac tgtagacaaa atggtcgtca tatctttagc
    34981 aacaaggatt ttgtcattaa attttccacg tccgtcttac aagctgataa gaaagaaatt
    35041 acgataatta ataaaacgaa a
//
LOCUS       DAJTYZ010000031        35003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000031 DAJTYZ010000000
VERSION     DAJTYZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.031"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..835)
                     /locus_tag="OO596_001448"
     CDS             complement(<1..835)
                     /locus_tag="OO596_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6717343.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GP"
     gene            927..1013
                     /locus_tag="OO596_001449"
                     /pseudo
     CDS             927..1013
                     /locus_tag="OO596_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1417..1893)
                     /locus_tag="OO596_001450"
     CDS             complement(1417..1893)
                     /locus_tag="OO596_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6717344.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1952..3241)
                     /gene="bioA"
                     /locus_tag="OO596_001451"
     CDS             complement(1952..3241)
                     /gene="bioA"
                     /locus_tag="OO596_001451"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6717345.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3328..4368
                     /gene="bioB"
                     /locus_tag="OO596_001452"
     CDS             3328..4368
                     /gene="bioB"
                     /locus_tag="OO596_001452"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6717346.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4365..5519
                     /gene="bioF"
                     /locus_tag="OO596_001453"
     CDS             4365..5519
                     /gene="bioF"
                     /locus_tag="OO596_001453"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6717347.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5506..6261
                     /gene="bioC"
                     /locus_tag="OO596_001454"
     CDS             5506..6261
                     /gene="bioC"
                     /locus_tag="OO596_001454"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6717348.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6254..6931
                     /gene="bioD"
                     /locus_tag="OO596_001455"
     CDS             6254..6931
                     /gene="bioD"
                     /locus_tag="OO596_001455"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6717349.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7510..9531
                     /gene="uvrB"
                     /locus_tag="OO596_001456"
     CDS             7510..9531
                     /gene="uvrB"
                     /locus_tag="OO596_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6717350.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9723..10631)
                     /gene="yvcK"
                     /locus_tag="OO596_001457"
     CDS             complement(9723..10631)
                     /gene="yvcK"
                     /locus_tag="OO596_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6717351.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10896..11040
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11028..12017
                     /gene="moaA"
                     /locus_tag="OO596_001458"
     CDS             11028..12017
                     /gene="moaA"
                     /locus_tag="OO596_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6717352.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12039..12551
                     /gene="moaB"
                     /locus_tag="OO596_001459"
     CDS             12039..12551
                     /gene="moaB"
                     /locus_tag="OO596_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6717353.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12554..13039
                     /gene="moaC"
                     /locus_tag="OO596_001460"
     CDS             12554..13039
                     /gene="moaC"
                     /locus_tag="OO596_001460"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6717354.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13032..13277
                     /gene="moaD"
                     /locus_tag="OO596_001461"
     CDS             13032..13277
                     /gene="moaD"
                     /locus_tag="OO596_001461"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6717355.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13279..13731
                     /gene="moaE"
                     /locus_tag="OO596_001462"
     CDS             13279..13731
                     /gene="moaE"
                     /locus_tag="OO596_001462"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6717356.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13868..14572
                     /locus_tag="OO596_001463"
     CDS             13868..14572
                     /locus_tag="OO596_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6717357.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14777..15490
                     /locus_tag="OO596_001464"
                     /pseudo
     CDS             14777..15490
                     /locus_tag="OO596_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15555..16266)
                     /locus_tag="OO596_001465"
                     /pseudo
     CDS             complement(15555..16266)
                     /locus_tag="OO596_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16266..17507)
                     /gene="clsB"
                     /locus_tag="OO596_001466"
     CDS             complement(16266..17507)
                     /gene="clsB"
                     /locus_tag="OO596_001466"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6717358.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17504..18265)
                     /locus_tag="OO596_001467"
     CDS             complement(17504..18265)
                     /locus_tag="OO596_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6717359.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18398..18808
                     /locus_tag="OO596_001468"
     CDS             18398..18808
                     /locus_tag="OO596_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6717360.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18770..19876)
                     /locus_tag="OO596_001469"
     CDS             complement(18770..19876)
                     /locus_tag="OO596_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6717361.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19887..21020)
                     /locus_tag="OO596_001470"
     CDS             complement(19887..21020)
                     /locus_tag="OO596_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6717362.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21013..22749)
                     /locus_tag="OO596_001471"
     CDS             complement(21013..22749)
                     /locus_tag="OO596_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6717363.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22742..23737)
                     /gene="hlyD"
                     /locus_tag="OO596_001472"
     CDS             complement(22742..23737)
                     /gene="hlyD"
                     /locus_tag="OO596_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6717364.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23740..24411)
                     /gene="cecR"
                     /locus_tag="OO596_001473"
     CDS             complement(23740..24411)
                     /gene="cecR"
                     /locus_tag="OO596_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6717365.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWIAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24640..26004
                     /gene="rhlE"
                     /locus_tag="OO596_001474"
     CDS             24640..26004
                     /gene="rhlE"
                     /locus_tag="OO596_001474"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR6717366.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26236..26718)
                     /gene="ybiA"
                     /locus_tag="OO596_001475"
     CDS             complement(26236..26718)
                     /gene="ybiA"
                     /locus_tag="OO596_001475"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6717367.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26838..28988
                     /gene="dinG"
                     /locus_tag="OO596_001476"
     CDS             26838..28988
                     /gene="dinG"
                     /locus_tag="OO596_001476"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6717368.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29016..29978
                     /gene="ybiB"
                     /locus_tag="OO596_001477"
     CDS             29016..29978
                     /gene="ybiB"
                     /locus_tag="OO596_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6717369.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30119..31204
                     /gene="hcxB"
                     /locus_tag="OO596_001478"
     CDS             30119..31204
                     /gene="hcxB"
                     /locus_tag="OO596_001478"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6717370.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31433..31693)
                     /locus_tag="OO596_001479"
     CDS             complement(31433..31693)
                     /locus_tag="OO596_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6717371.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31958..32224)
                     /gene="ybiI"
                     /locus_tag="OO596_001480"
     CDS             complement(31958..32224)
                     /gene="ybiI"
                     /locus_tag="OO596_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6717372.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32298..32975)
                     /gene="ybiX"
                     /locus_tag="OO596_001481"
     CDS             complement(32298..32975)
                     /gene="ybiX"
                     /locus_tag="OO596_001481"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6717373.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33017..35003)
                     /locus_tag="OO596_001482"
                     /pseudo
     CDS             complement(33017..35003)
                     /locus_tag="OO596_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8276 a         8981 c         9498 g         8248 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg
       61 acaatggatt gttataaata ttaacatctg cataaatgtg cagattcatg atactgtctg
      121 gcaatgtgtt cagctgattg ttagggatat atagtgattg cagacgttgt ggaagttcgg
      181 gcagtctgtg tagttggttt tccccaacgt ttaaaagcct cagattttga ggaaggggcg
      241 gtagctcttg taatctgtta tcaaaagcac ttagttcctg tagttcacat ggtaattctg
      301 gtaaggcagt aagctggttg ttgccaacag aaagtttttg tagagccggg ggaagatcag
      361 gtagcgtctc caggctattg agaagggctg agagtgactg caaggaagat ggaagagaag
      421 gcaaacatgt taaaactctg ttaagtgaaa catcaagagc gaccaggtga ggcggaagag
      481 ccgggagcct gctcagtctg ttatcgctgg ctttaagtac agttaaggac ggagggagtt
      541 ctggcagaga gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt
      601 atgggagaga gcgtaatctg agtaaactta aattgagggc tggctcttga aaagccagac
      661 atattttcag taatcgaact gcctgtgttc ggtcttctgt tgcagcacct tccttggccc
      721 agttatccca gatgcggtga taatccgtga tattttgctc ttctacggat aaacgggata
      781 tacatcctaa accacaaaag gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt
      841 attgatcctc attgtttata tattaatatg ttcgtgcaaa tggagtgagg attctttaaa
      901 aaaacgcgcc tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa
      961 atgaaggggt gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc
     1021 cctacctttt catcaagcca gtagtctgcc caccactgca tcatttctct gcgcttatcg
     1081 agatactgag catggttgta aattcagcta acccagtgcg ttgacaaaaa attagcgcaa
     1141 gaggacaaaa aatcaccttg cgctaatgct ctgtatcagg tcactaatac catctaagtg
     1201 gttgattcat agtgactgga tatgttgtgt tttgcagtat tatgcagtct attttttaag
     1261 ctaaatgtat tataatgcat tgatattaat gatttttact gtttctcgtt cagcttttta
     1321 tactaacttg agcgaaacgg gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag
     1381 tgataccaga tggcattgcg ccatctggca gagtgattaa ctaaacatcg cagtaatcga
     1441 ggcgcttgcc agagagtgga aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc
     1501 atcgacatca atacgttcta tatccagcgc gtgaacggta aaaatgtagc gatgagtttc
     1561 gcctttcggc ggtgctgcgc catcgtaccc ggttttacca aagtcggtac gcgtctgcaa
     1621 aacgccgtct ggcattgcta ccagaccaga gccaaaccct tgcggtaata cgcgggtatc
     1681 agcgggtaag ttaacaacta cccagtgcca ccagccggag ccggttggcg catccgggtc
     1741 gtagcaggtg acaacaaaac ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg
     1801 tgaaatatta tcgccatcgt aacccatgcc gttaaagaca tgacgatgcg gcaatttatc
     1861 gccatcgcgc agatcgttac tgatgagttt catgaaccct cctttcttgt ttgcagaaag
     1921 tgtagccaga aaccctcacg cggacttctc gttattggca aaaaaatgtt tcatcctgta
     1981 ccgcgcggtt aaccgctgcg gtcagacgct gcaactgttg cgggagaata atatagggcg
     2041 gcatcaggta aatcagtttg ccaaaaggcc ggatccagac accctgttcg acaaagaatt
     2101 tttgcagcgc cgccatattc accggatgag tggtttcgac cacgccaatg gcccccagta
     2161 cgcgcacatc ggcaaccatt tcggcatcac gggcgggggc aagttgctcg cgcagctgta
     2221 cttcaatatc cgccacctgt tgctgccagt cgccagattc gagaatcgcc aggctggcgt
     2281 ttgctgccgc gcaggccagc ggattgccca taaaagttgg cccatgcata aagcaaccgg
     2341 cttcaccgtt actgatggtt tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca
     2401 ttgtgccgcc ggttaaggct ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt
     2461 cacaggcaaa cagtttcccg gtacgaccaa atccagtggc gatctcgtcg gcaatcagca
     2521 agataccttc gcgatcgcat attttgcgga ttcgttttaa ccattccgga tggtacatgc
     2581 gcatcccgcc tgcgccctgg acaatcggct caatgatcac cgccgcgatt tcatgacgat
     2641 gcgccgccat caggcgggca aagcccacca tatcgcgctc atcccattcg ccatccatac
     2701 ggctttgtgg ggcgggagca aacaagtttt ctggcagata gcctttccac aggctgtgca
     2761 ttgagttgtc cggatcgcac accgacatcg cgccaaaggt atcgccatga taaccattgc
     2821 ggaaggtcag aaaacgctgg cgcgcttcgc ctttggcttg ccagtactgc aacgccattt
     2881 tcatcgccac ttccaccgct acggaaccgg agtccgcgag aaaaacgcac tccagcggtt
     2941 gcggcgtcat cgccaccagt ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc
     3001 caaacatcac atgcgacatg gcatcaattt gcgacttcat cgccgcatta agctgcgggt
     3061 gattgtagcc gtggatcgcc gcccaccagg acgacatacc gtcaaccagg cgtctgccgt
     3121 cagacaaaat cagctcgcaa ccttcggcgc tcaccaccgg ataaaccggc agcggggagg
     3181 tcatggatgt gtatgggtgc cagatatggc gttggtcaaa ggcaagatcg tccgttgtca
     3241 taatcgactt gtaaaccaaa ttgaaaagat ttaggtttac aagtctacac cgaattaaca
     3301 acaaaaaaca cgttttggag aagccccatg gctcaccgcc cacgctggac cttgtcgcaa
     3361 gtcacagaat tatttgaaaa accgttgctg gatctgctgt ttgaagcgca gcaggtccat
     3421 cgccagcatt tcgatcctcg tcaggtccag gtcagcacgt tgctgtcgat taagaccgga
     3481 gcttgtccgg aagattgcaa atactgcccg caaagctcac gctacaaaac cgggctggaa
     3541 gccgagcggt tgatggaagt tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca
     3601 ggatcgacgc gcttctgtat gggcgcggcg tggaagaatc cccacgaacg cgatatgccg
     3661 tacctggaac aaatggtgca gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg
     3721 ggcacgttga gtgaatctca ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac
     3781 cacaacctgg acacctcgcc ggagttttac ggcaatatca tcaccacccg cacttatcag
     3841 gaacgcctcg atacgctgga aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc
     3901 attgttggct taggcgaaac ggtaaaagat cgcgccggat tattgctgca actggcaaac
     3961 ctgccgacgc cgccggaaag cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca
     4021 ctggcggata acgatgatgt cgatgccttt gattttattc gcactattgc ggtagcgcgg
     4081 atcatgatgc cgacctctta cgtgcgcctt tctgccggac gcgagcagat gaatgaacag
     4141 actcaggcga tgtgctttat ggctggcgca aactcgattt tctacggttg caaactgctg
     4201 accacgccga atccggaaga agataaagac ctgcaactgt tccgcaaact ggggctaaat
     4261 ccgcagcaaa ctgccgtgct ggcaggggat aacgaacaac agcaacgtct tgaacaggcg
     4321 ctgatgaccc cggacaccga cgaatattac aacgcggcag cattatgagc tggcaggaga
     4381 aaatcaacgc ggcgctcgat gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg
     4441 tggcgcaagg agccggacgc tggctggtgg cggatgatcg ccagtatctg aacttttcca
     4501 gtaacgatta tttaggttta agccatcatc cgcaaattat ccgcgcctgg cagcagggcg
     4561 cagaaaagtt tggtgtaggt agcggcggtt ccgggcatgt cagcggttat agcgtggtgc
     4621 atcaggcgct ggaagaagaa ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta
     4681 tctctggttt tgccgcgaat caggcagtca ttgccgcgat gatggcgaaa gaggaccgta
     4741 ttgttgccga ccggcttagc catgcctcct tgctggaggc tgccagttta agcccgtcgc
     4801 agcttcgccg ttttactcat aacgatgtcg ctcatctggc gcgactgctt gcttccccct
     4861 gtccggggca gcaactggtg gtgacagaag gcgtgttcag catggacggc gatagcgcgc
     4921 cactggcgga aatccagcag gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg
     4981 cccacggcac gggcgttatc ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa
     5041 aaccagaatt gctggtggtg acttttggca aaggatttgg cgtcagtggg gctgcggtgc
     5101 tttgctccag tacggtggcg gattatctgc tgcaattcgc ccgccacctt atctacagca
     5161 ccagtatgcc gcccgctcag gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg
     5221 atgaggggga tgcacggcgc gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag
     5281 tacaggatct gccgtttacg cttgctgatt catgcagcgc catccagcca ttgatcgtcg
     5341 gtgataacag ccgtgcgtta caactggcag aaaaactgcg ccagcaaggc tgctgggtca
     5401 cggcgattcg cccgccaacc gtacccgctg gtactgcgcg actgcgctta acgctaaccg
     5461 cagcgcatga aatgcaggat atcgaccgtc tgctggaggt gctgcatggc aacggttaat
     5521 aaacaagcca ttgcagcggc atttggtcgg gcagccgcgc actatgagca acacgcagag
     5581 ttacagcgcc ggagtgctga cgtcttactg gcaatgctcc cacagcgtaa atacacccac
     5641 gtactggacg cggggtgtgg gccaggctgg atgagccgcc actggcgaga gcgtcacgca
     5701 caggtgacgg cgttagatct ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct
     5761 gcagaccatt atctggcggg agatatcgaa tccctgccgt tagcgactgc gacgttcgat
     5821 cttgcatgga gcaatctcgc ggtgcagtgg tgcggtaatt tatccacggc actccgcgag
     5881 ctgtatcggg tggtgcgccc cggaggcgtg gtggcgttta ccacgctggt gcagggatcg
     5941 ttacccgaac tgcatcaggc gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt
     6001 ttaccgccag atgaaatcga acagtcgctg aacggcgtgc attatcaaca tcatattcag
     6061 cccatcacac tatggtttga tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt
     6121 gctacgcatc ttcatgaagg gcgcgacccg cgaatattaa cgcgttcgca attgcagcga
     6181 ttgcaactgg cctggccgca acaacagggg cgatatcctc tgacgtatca tctttttttg
     6241 ggagtgattg ctcgtgagta aacgttattt tgtcaccgga acggataccg aagtggggaa
     6301 aaccgtcgcc agttgtgcac ttttacaagc cgcgaaggca gtaggttatc ggacggcagg
     6361 ttataaaccg gtcgcctctg gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc
     6421 gctggcgtta cagcacaaca gcagcctgca gctggattac gcaacagtaa atccttacac
     6481 cttcgcagaa cccacttcgc cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc
     6541 attggtaatg agcgccggat tacgcgcgct tgaacaacag gctgactggg tgttagtgga
     6601 aggtgctggc ggctggttta cgccgctttc tgacactttc acttttgcag attgggtaac
     6661 acaggaacaa ctgccggtga tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc
     6721 gatgttgact gcgcaggcaa tacaacacgc cggactgact ctggcgggtt gggtggcgaa
     6781 cgatgttacg cctccgggaa aacgtcacgc tgaatatatg accacgctca cccgcatgat
     6841 tcccgcgccg ctgctgggag agatcccctg gcttgcagaa aatccagaaa atgcggcaac
     6901 cggaaagtac ataaaccttg ccttgttgta gccattctgt atttggttaa attgcgagcg
     6961 agatcgcgtc ttcgattgac tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa
     7021 aattttactt ctgcctcatg cggcgaatgt gggaattgcc caggcggcgg gggatagggg
     7081 ctggagacag ttatccacta ttcctgtgga taaccatgtg tattagagtt agaaaacacg
     7141 aggcaagcga gagaatacgc ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa
     7201 tatcttttat tttttaactg gttagataaa tgcaatggca gtcactgaac aggcatctct
     7261 tgccataaaa ctgtcatcac tcatcttgac aaatgttaaa aaagccgttg ctttggggat
     7321 aacccggtaa ggccggagtt ttatctcgcc acagagtaaa ttttgctcat gattgacagc
     7381 ggagtttacg ctgtatcaga aatattatgg tgatgaactg tttttttatc cagtataatt
     7441 tgttggcata attaagtacg acgagtaaaa ttacatacct gcccgcccaa ctccttcagg
     7501 tagcgactca tgagtaaacc gttcaaactg aattccgctt ttaaaccttc tggcgatcag
     7561 ccagaggcga ttcgacgtct cgaagagggg ctggaagatg gcctggcgca ccagacgtta
     7621 cttggcgtga ctggctcagg gaaaaccttc accattgcca atgtcattgc tgaccttcag
     7681 cgcccaacca tggtacttgc gcccaacaaa acgctggcgg cccagctgta tggcgaaatg
     7741 aaagagttct tcccggaaaa cgcggtggag tatttcgtct cctactacga ctactatcag
     7801 ccggaagcct atgtaccgag ttccgacact ttcattgaga aagatgcctc ggttaacgaa
     7861 catattgagc agatgcgttt atccgccacc aaagcgatgc tggagcggcg tgatgtggtt
     7921 gtagtggcct ctgtttccgc gatttatggt ctgggcgatc ctgatttata tctcaagatg
     7981 atgctccatc tcacggtcgg tatgattatc gatcagcgcg cgattctgcg ccgactggcg
     8041 gagctgcaat acgctcgtaa tgatcaagca ttccagcgtg gtactttccg cgttcgtggc
     8101 gaggtgatag atatcttccc ggcagaatcg gatgacattg cacttcgcgt ggaactgttt
     8161 gacgaggaag tggaacgatt gtcgttattt gacccgctga ccgggcagat tgtttccact
     8221 attccacgtt ttaccatcta cccgaaaacg cactacgtca caccgcgcga gcgcatcgta
     8281 caggcgatgg aggagatcaa agaagaactg gccgccagac gcaaagtgct gttggaaaac
     8341 aacaaactgc tggaagagca gcggctgacc cagcgtaccc agtttgatct ggagatgatg
     8401 aacgagctgg gctactgttc ggggattgaa aactactcgc gcttcctctc cggtcgtgga
     8461 ccgggtgagc caccgccgac gctgtttgat tacctgcctg ccgatgggct gctggtggtc
     8521 gatgaatctc acgtcaccat tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt
     8581 aaagagacac tggtggagta cggcttccgc ctgccatcag cgctggataa ccgtccgctt
     8641 aagtttgaag agttcgaagc attagcgccg caaaccatct atgtttcggc gacgccgggt
     8701 aattacgagc tggaaaaatc cggcggcgat gtggtggatc aggtggtgcg tccaaccgga
     8761 ttgcttgacc cgattatcga agtgcggccg gtggcgacac aggttgatga tcttctttcg
     8821 gagattcgtc agcgagcggc aattaacgaa cgcgtactgg tcaccacact gaccaagcgg
     8881 atggcggaag atcttaccga atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat
     8941 tcagatatcg acaccgtcga acgtatggag attatccgcg acttgcgtct gggtgagttc
     9001 gacgtgctgg tagggatcaa cttactgcgc gaaggtctgg atatgccgga agtgtcgctg
     9061 gtggcgatcc tcgacgctga caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag
     9121 actattggtc gtgcggcacg taacgttaac ggtaaagcga ttctctacgg cgataagatc
     9181 accccatcaa tggcgaaagc gattggcgaa accgaacgtc gccgtgagaa acagcagaag
     9241 tacaacgagg aacacggaat tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg
     9301 gcgctggggc agaacattgc caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt
     9361 gttgagccgg ataatgtgcc gatggatatg tcgcctaaag cgttgcaaca gaaaatccat
     9421 gagctggaag ggttaatgat gcaacacgcg cagaatctgg agttcgaaga agcggcacaa
     9481 attcgtgacc agttgcatca gctgcgtgag ctgtttatcg cggcttcgtg acaggataac
     9541 gaagaagaca gatgacaaaa gcaaaattgc ctgatgtgct acgcttatca ggcctacatg
     9601 atctctgcaa tacattgaat tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat
     9661 ccggcataaa caacgcgcac tttgtcttca ataggaagcc ggaatttttc ttcggatttc
     9721 cgttaaccta aagcctgtaa cgccttttcc agcgcgttat gtaacaactg gcggtcatga
     9781 cgatacggaa tatcgctggc ctccagtacc tcctgaatca caatccgctc tttcaccgcc
     9841 gagacatcca cttttggccc gacgatgacc gcatcaatga cctttttacc aacatactgc
     9901 tccataattg ccagcttgct ttccagcttc aaattagccg caggtaaact caactcacgc
     9961 cccagattgc cgatatagac catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc
    10021 ttcagcagca gaattggcat caggctggta taaaaactgc caggcccaat aatgatgaga
    10081 tccgcttcat tgatagcgtg aaccgcctca cgcgttgcgg gtacattcgg cgttaataat
    10141 aactcttgaa tcggcgtagt taactggtcg atattgacct cgccgtaaac ttcatgcccc
    10201 tgatcgtcaa tcgccatcag atcgacggga tgctctgaca ttggaatcaa atgcgtatcc
    10261 actttcagca gattacgaat taaattgatg gcttccagag gccgcacgct aaggtgatcc
    10321 agcgccttta acatcaagtt tccgagatta tgaccggaaa gttcgccatt gccgccaaaa
    10381 cggtattcaa acatcgcgga ggcgacgctc ggttccgtta tcagttggtt gaggcagttg
    10441 cgcatatcgc cccaggcaat gccgccttct gaacggcgaa tacgccccgt cgagccacca
    10501 ttatccgtgg tggtgacgat acccgttaaa cgagaaccca aagacgaaag tgatgagaga
    10561 acgcgtccca gtccatgccc tccgccgaga gcaacgacac gatcaagatc agccagcgta
    10621 cgattgcgca tatagtttcc tggagtcaga ttatccgcgc tacagtagcg caaattgcgg
    10681 ggaaacagca attaacctgc caatattgag gatacaaact attttcttct acctctaaag
    10741 gacgatgcac gctatgcctc cctgatgatg tatatcaaag taaaactgcc attttccctt
    10801 attctgtagc gaaatagcac gatcatgacg ctatatacat gattacatag cgaaagtgtg
    10861 gatggtaaaa atctcattta cacgctagta tcggcttaac cactaaacac tctagcctct
    10921 gcacctgggt caactgatac ggtgctttgg ccgtgacaat gctcgtaaag attgccacca
    10981 gggcgaagga agaaatgact tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac
    11041 tgactgatgc atttgcgcgt aagttttact acttgcgcct gtcgattacc gatgtgtgta
    11101 actttcgttg cacctactgc ctgccggatg gctacaaacc gagcggcgtc accaataaag
    11161 gctttcttac cgtcgatgaa attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg
    11221 aaaaagtgcg cctgacagga ggagagccgt ctttacgccg cgactttacc gatatcatcg
    11281 ccgctgtgcg ggaaaacgac gctatccgcc agattgcggt caccaccaat ggttaccgtc
    11341 tggaacgcga tgtggcgaac tggcgcgatg cgggacttac tggcattaac gtcagtgtcg
    11401 acagtctgga cgcccgccag tttcacgcta ttaccgggca ggataaattc aaccaggtca
    11461 tggcagggat cgatgctgca tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc
    11521 tgatgcgtga tgttaatcat caccagctcg acacctttct gaactggatc cagcatcgcc
    11581 ctatccagct gcgtttcatc gaactgatgg aaacgggcga gggcagtgag ctcttccgta
    11641 agcatcacat ctctggtcag gttctgcgtg acgagctact gcgtcgcggc tggatccacc
    11701 aattacgtca acgcagcgac ggtcctgcgc aagtcttttg ccatccagat tacgccggag
    11761 agattggcct tatcatgccg tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg
    11821 tttcctccat tggtaaactc catctctgcc tgtttggtga aggcggcgtt aacctgcgcg
    11881 atctgctgga agacgatacc cagcaacagg cgctggaagc gcgtatttca gcggcgctgc
    11941 gggagaagaa acagacccat ttcctgcatc aaaacaacac cggtattacg caaaacttat
    12001 cgtacattgg cggctaaaac gtcaaaagga gagatcagat gagtcaggta agcactgaat
    12061 ttatcccgac ccgtattgct attcttacgg tttctaatcg tcgcggtgaa gaagacgata
    12121 cctccggtca ctatttgcgc gattcggcgc aagaagcggg ccatcacgtt gtcgataaag
    12181 ccattgtgaa agaaaaccgc tacgctattc gcgctcaggt atctgcgtgg atcgccagcg
    12241 acgatgtaca agtggtgttg attacggggg ggactggcct gacggaaggt gatcaggctc
    12301 ccgaagcatt gctgccgttg ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga
    12361 tgttgtcgtt tgaagagatt ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg
    12421 ccaacaaaac gctgattttc gccatgccgg gttcgaccaa agcgtgccgt accgcatggg
    12481 aaaatatcat cgcgccgcag ctggatgccc gtacgcgtcc gtgtaatttc catccacatt
    12541 tgaagaaata agtatgtcgc aactgaccca tatcaacgcc gctggcgaag cgcacatggt
    12601 ggatgtctcc gccaaagcgg aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat
    12661 gcgcagcgag acgctggcga tgattattga tggtcgccac cacaaaggcg acgtatttgc
    12721 cactgcgcgt attgtcggta ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg
    12781 tcacccgctg atgctcagca aagttgaagt caatttacag gccgagccgg agcacaatcg
    12841 ggtgcgtata gaaaccttat gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt
    12901 aaccgcggcc tccgtggcgg cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga
    12961 tatggtgatt ggtccggtac gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa
    13021 ggtggaagcg gatgattaaa gttctttttt tcgcccaggt gcgcgagttg gtgggaacag
    13081 atgcaaccga agtggctgcg gattacccaa ccgttgaagc gttacgccag cacctggctg
    13141 cgcagagcga tcgctgggcg ctggcgctgg aagatggcaa attgctggct gccgtcaacc
    13201 agacgctggt gagttttgac catccgctga ctgacggcga cgaagtagct ttcttcccgc
    13261 cggtaaccgg aggttaagat ggcagaaacc aaaattgttg tcggcccgca gccgttcagc
    13321 gtaggagaag agtacccgtg gctggctgag cgtgacgaag acggtgcagt ggtcacgttt
    13381 accggtaagg tgcgcaacca taacctgggt gacagcgtca aagcattaac cctcgaacac
    13441 tatccgggga tgactgaaaa agcactggca gaaatcgttg atgaagcgcg taaccgctgg
    13501 ccgctggggc gcgtcacggt gattcaccgc atcggggaat tatggccggg cgatgaaatc
    13561 gtttttgtcg gtgtcaccag tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt
    13621 atggattatc tcaaaacccg cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac
    13681 cgctgggttg aagcccggga gagcgatcag caggcggcaa aacgctggta gttttttgtt
    13741 agccggataa ggcaccgctg cgtccggcaa tttcaccgct cagtacatgc gtttacgctg
    13801 cgtcgatgca cagcctcatc attttgcagt atccttaaga tattccttat atcttcagga
    13861 gatcgtcatg gacagattcc cacgttctga ttcaatcgta caaccccggg ctggcttgca
    13921 aacttatatg gcgcaagtct atggctggat gaccgttggc ttgttgctga ccgcatttgt
    13981 tgcctggtat gcggctaatt ccgcggccgt gatggagctg ttgttcacta accgtgtctt
    14041 tttaatcggt ctgatcatcg cgcaattagc attggttatt gtgttatcag cgatgattca
    14101 aaagctgagc gcaggtgtaa cgacgatgct ctttatgctt tattcggcgc tgacgggtct
    14161 tacgctttcc agtatattca ttgtctatac cgctgcttct atcgccagta ctttcgtcgt
    14221 tactgccggg atgttcggcg caatgagcct gtacggttac accacgaagc gcgatttaag
    14281 tggctttggc aatatgctgt ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa
    14341 cttctggctg aaaagcgaag cattgatgtg ggcagttacc tacatcggcg tgattgtctt
    14401 tgtcggattg acggcgtatg acactcagaa attgaaaaat atgggtgagc agattgatac
    14461 ccgcgacacg tcgaacctgc gcaaatattc cattcttggc gcgttaacct tgtatctgga
    14521 cttcatcaac ctgttcctga tgttgttacg gatcttcggc aaccgccgtt aattcttttt
    14581 caccatcact atcccggcca tgtgtcggga tttttttctt actaaaaacg acacttaaca
    14641 agtaccaggt ataaaatatg ccttatgagc gagcggaatc gttttccgta tccataaata
    14701 caaaagtatt ttgtatgcgt ggttttaaat ttattgatgg tgaatattaa tattttttct
    14761 aaggacaact aaaagaatgg aatcatactc gcaaaacagc aataaattag attttcagca
    14821 cgaggccagg atataaaacg gtatatggct cattaccgct ttaggtttgg tggcaaccgc
    14881 aggactagcc tggggagcta agtatctcga aattacggca accaaatatg attcaccacc
    14941 aatgtatgtc gctatagggt tattattact ttgtatgtat ggcttaagta aggatatcaa
    15001 caagataaat gccgccatcg cgggcgtaat atatctgttt ttactctctt tggtggcgat
    15061 tgtcgttgca agtttagttc ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc
    15121 gatgttttta atcagtatgc tggccggttt attatttaat gttgagcctg gttctcaccg
    15181 ttttatcatt atgatgacgt tgacagggtt ggccctggta atcatcgtga atgcggcatt
    15241 aatgagtgaa cggcccattt gggtaataag ttgcttaatg attgtgttat ggccaggcat
    15301 tatctcgcat ggacgaaata agctccttga actggcagga aaatgccata gtgaagagtt
    15361 gtggagtccg gttcgttgcg cttttacagg tgcattaaca ctctattact attttatcgg
    15421 cttctttggg atacttgccg cgatagctat aacgcttgtc tggcaaaggc atacgcgttt
    15481 ttttcattag ccgtaaatgc acatactaaa gaacttaact aaacttcaca tcgccgcttc
    15541 atttttcgcc cgtagcccag tacaaagaaa tagttcacgc tttgaccaag taacagccag
    15601 ataatcgccc ccattaccat ccagttaacg ctggaaatca gcatctgagc gagggcgaat
    15661 ttccatgaag gcagcaccag cttttgccct ttgatggtca tatggcggtg cttcgcgaaa
    15721 gcgcaaaacc acaaataaac cgcgataatc atcagtaagc caatgccgag aatacgcagc
    15781 gtagtttgat cgacatacca gtgatccggc aactccacca cgcctgcggt aaagataatc
    15841 cctgccagta aaatatagcc cagccagttg gtggtaatac tgagcgagaa aatccgcgta
    15901 atagtgctac cgggcaaccc cagacgagag tacaaacgat agcgcatacc aatgccgccg
    15961 acccaggtac tgagcgtcag gttgaaggcg tagcagataa acgacaccag catcacctgg
    16021 cgcttcgcca gtttgtgacc gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc
    16081 agatagctga cgaccaccag cgaccgcact aagcagcgca acgcgattgt agtcgcggat
    16141 gaccttccag acctcttccc agtccacttt tttggcgtag accaccagca acacaatcac
    16201 cgcgataaaa aacagccagg tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt
    16261 actcatcagg gttttacccc cgtgttttca gtttctaccc gatcctgcgt ttccattgtc
    16321 ggttgtgcgg gcggatcaac ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc
    16381 agcgccggga agtggcgtaa aaagtggaac gccagcacgc ttttggtcag gttccaccag
    16441 gtgcgtttgg gcagcatggt ttcatccacc tgctgacaat ctgcggcgat aatgccgttc
    16501 agattatcgc gcagcgtctg gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga
    16561 ttcagtgaca aactgagcgg atcgagatta ctggacccta ctgtcgccca gtgatcgtcc
    16621 atcaatgcta ctttgccgtg gagcgggcgg cggcggtact caaacacctg aacgccgcct
    16681 ttaaccagat agttatacag caagcgcgcg ccgactctga caatcggcat atccggttcg
    16741 ccctgaatga tcagtttgat ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa
    16801 aatcgatagc cggggaagaa gtaggcgttg gcgataatga cttcccgccg cgcctgagtg
    16861 agcattttca gatagtggcg ttctatatca tcgcggtgtt cttcgttatc gcgccagacc
    16921 agcaatacct gcgcgtctcc cggctggcgg ttctcttccg ctttgtgatg acgtcgccac
    16981 cagcgtcgtg ccgcgctctg tccaggcagg ttttccagct caaactggag aatatcttcg
    17041 acaatcggcc cttcaaggcg taccgcgtaa tcctgtttag cctctggacc gtagctggac
    17101 atatgctcgg cggagtaatt cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca
    17161 attttgcgat gcatccggcg aaacacattg gtgcgcatac caaaaaggcg agggcgggga
    17221 tcgtagtagc ggaacactac gccagctgcc gtcagttcat tgacaaactc atcgctgaga
    17281 tccggcgaac cgtagccatc cagcaagact tccgctttaa ccccgcgttg cgctgctgcc
    17341 agtagtgccg catgcagctg tttgccgacg ttatcctcaa accagataaa cgtttcaaga
    17401 atgatgcgtt cttgtgcctc gccaatcgcc ttaaacaccg cgggataata ttgctcgccg
    17461 ttttccagca actggatctt attgccttcg cgccagctac atttcataaa tgaatctccg
    17521 cacttaaagg ggcatgatca gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg
    17581 cgctggcgct ggcatttttg acgtagatcc tgtccagtcg tagtagagga aattgcaccg
    17641 gaaacgtgcg cgccgggcgt ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca
    17701 cttttaacgg atgattagct ttttgccgcc agtcattgaa atcacccgcc accaataccg
    17761 gttcgccgtc cggtagctca ttcacccatt cggcgagcat cgcaagctgc gcctgacggt
    17821 gcgcctcacg caggcccaga tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg
    17881 gcacaatgcg gcagtagagc acgccgcgct tttccgcacc atcgaccgaa acatcgcgat
    17941 tctcataatg ttcaatggga taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt
    18001 atacggcatt gcgaccgtag gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt
    18061 gcgaggtatc gggccagttt tccacatgca gcggatgaac ttcgtgcgcg cccatcactt
    18121 cctgcaggca aacaatatcg gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa
    18181 tgaagcgtcg gttaaacgcg gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg
    18241 aaaattgttg tgtttgatcg ggcataattt tcctgtcttt gcctcttatc tcattgaaat
    18301 agtgtagtcg gcgtcacaaa aaggtgcggt cttacggaat tttccgtaaa gttcggtact
    18361 ctgagtaagt agagataaat tcttcaggag agaagccatg aagtggcaac aacgtgttcg
    18421 tgtcgcaacg ggtctaagtt gctggcagat tatgttgcat ttactggtag tggcgctgct
    18481 ggtggtgggc tggatgagta agactctggt tcacgtcggc gtgggattat gcgcactgta
    18541 ttgtgtcacg gtagtgatga tgctggtgtt tcagcgccac cccgagcaac gctggcgtga
    18601 ggtggcagac gtgctggaag agctgaccac gacctggtat tttggcgcag cgctgattgt
    18661 gctgtggctg ttgtcccgcg tactggaaaa caactttttg ctggcaattg cagggctggc
    18721 gatccttgct ggcccggcgg tagtgtcttt gctggcgaaa gataagaagt tacatcacct
    18781 tacgtctaaa catcgcgtac gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc
    18841 acaaactatt ccacacaata tccagactcg catccttcaa ataaatctgc ttggtaatgt
    18901 ccgtaaagtg gcgaataggg ttaatccacg tcaggttttg cagccatact ggcatgtttt
    18961 ccaccggaga aacgtaaccg gaaaggagaa tggcgggcat cataaaaaca aacacgccga
    19021 taaacgcctg ctgttgtgtc gagcagagtg atgaaatcaa cagaccgaat cccaccagcg
    19081 ataaaccgta aatcaccatc gtaaagtaga acagcgccag cgatccggcg aaggggattt
    19141 gatacgccca gataccaatc gccagcacaa tggtggcctg gaaggtggcg acaattaacg
    19201 ccggtacggc tttgccgatg aagatctgcc aggtggtgag cggcgaaacc agtagctgat
    19261 cgagcgtacc ttgttcacgt tcgcgggcga cggaaagtga agtgacgatc attacgccga
    19321 tagtggtgat catggcgatc agcgacggca ccacaaacca tttgtagtca agattcgggt
    19381 tataccagtt gcgtaccacc agctcgctgt tgttaggttt cggttttcct tccagcagct
    19441 cctgctgata atttttgacg atctgttgca ggtagttggc ggcaatttgc gcactgttgg
    19501 agttacgccc gtcgaggatc aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg
    19561 agaagtcagc cgggaaacgc accagtagta acgccttttg tgtgtcgatg gttgggcgga
    19621 tctcctgtgg gcttttcagc agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt
    19681 gggtcagctc caccgaatgc tcgccgttat cttcatcgta gatggcgatg gtggcgttag
    19741 tcacttccag cgtggcggcg aacgggaaca ggatcacctg aataagcaca ggtaaaatca
    19801 gaatcgcgcg ggtttgtggt tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc
    19861 ataagcgatg aaacatgctc ttctccctaa tccagccgac gtttggtttt cagccacgtc
    19921 aggccgataa acatcaccgc cgaagcgatc aaaaacagca cgtttaccac cagcaccact
    19981 ggaatattcc cggcgaggaa caggctttgc agggtgctga cgaaataacg agcgggaata
    20041 atgtacgtca ccgcgcggat caccgcgggc atactgtcga tctgaaaaat aaagccggaa
    20101 agcataatcg acggcaggaa cgcggcgttc agggcgacct gagcggcatt gaactggttg
    20161 cgggtaatcg tggaaatcag cagccccatc cccagggtac tgagtaaaaa caggctggag
    20221 ataaaaaaca gaataagcag cgaaccgcga tacggcacgc cgagaataaa cactgacacc
    20281 agcatacaca gcagcatcgc cagcatcccg agaaagtaat aagggatcag cttacacagc
    20341 agcagttccg tgcgggtaat ctccgtagag agcagagcct ccatggtgcc gcgttcccat
    20401 tctcgcgcca ccaccagcga ggtgagaatc gcgccgatga ccgtcatgat gatggtcact
    20461 gcaccgggga taatgaagtg ctggctaatc gccgccgggt taaaccagta gcgggtttgt
    20521 acatcaataa gcggttcaaa agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc
    20581 tgccagatcc cttcgacata cccctgtaca aagttagcgg tattcggctc actgccgtcg
    20641 gtgatcacct gaatcggtgc ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc
    20701 ggaataacca ccagaccgcg aattttcccc gcctgcattt tggcgatcag ttcctgacgg
    20761 ttatcgctga tggtggcgtc gatgtagggc gaaccggtca tggtgtgggt gaaatccagc
    20821 gccgcttcgc tacgctgttc cagcaagatc ccgacccgca gcttgctgga gtcgaggtta
    20881 atgccataac caaaaataaa cagcagtagc agtgggatca ctaccgcaat cagccagcta
    20941 ctcggatcgc gaacgatctg ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag
    21001 gaaaggatcg ggttactcat tgctatgctc cttatcccag tcgtggatca actgaataaa
    21061 ggcttgctcc atggtgggat cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc
    21121 cggcgtaccg ctggcgatta atttcccacg gtacaccagg ccgatgcggt cgcaatattc
    21181 cgcttcatcc ataaagtggg tggtgaccat caccgtgacg cctttctcta ccatgctgtt
    21241 gatatgtagc caaaattcac ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag
    21301 aaacagaatg tccggttcat gcatcagcga acaggccagc gccagccgct gtttaaaacc
    21361 taatggcagt tcatcggtgg cgtgggaggc gatacttttc aggccgaacg cctcgctcat
    21421 gcgggagatt ttttcgttct gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg
    21481 taaattctgt tcgaccgtca ggttaccgta gagcgaaaac ttttgcgcca tatagccgag
    21541 atgctggcgc gctttaccag aactctcttt cagatccatc cccagtacca gcgcctgacc
    21601 agaggtcggc accagcaaac cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt
    21661 tggccccagc aaaccaaaaa tctccccgtg tttaacggca aagttgacgt gatcggtggc
    21721 ggcaaaatca ccaaatttct tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt
    21781 gccttctacc gtatgtaata ttgcgcccag cggcgattcc gaggttccgg caccgcccag
    21841 caaatcaata aacgcatctt caaaacgcgg cgtagtttcg ttgatgttga tttccggcat
    21901 cccgtcggca tggcgaatat cgtctggtgt ggtctctttt ttgaggatca gacgtaccga
    21961 tttcccctga atcatgccgt cgctgacctg cggcagtttc aatgcccgtt gcaacagttt
    22021 gcggttgccc tcgtgtggac ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag
    22081 ggcttttggt tccccctgat acagcaactc gccttcattc atcagtaaca cgtcacggca
    22141 ctgctcggct tcgtcgagat acgaggtact ccagaggatt aacatccctt cgcctgccag
    22201 ctcatgcacc atctgccaca gttcgcgccg tgagataggg tcaacgccga cgccgggttc
    22261 atcgagcagc aacactttcg gttcgcccac cagggtacag gccaggccga gtttttgttt
    22321 catcccaccg gagagcttgc ccgccaggcg tccggtaaac ggcccaagag acgtaaactc
    22381 cagcaggcga gcaaaagttt gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta
    22441 cagattgagg ttctccatca ccgtgagatc ttcatacaga ccaaatttct gcggcatata
    22501 gccgagcacg gcgtgcagcg cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc
    22561 attgccgctg tcgggtttca gtaatcccgc cagcatccgc attagcgtgg ttttacctgc
    22621 accatccggc cccaccagcc ccgtcacata accggcgtga atggtacaat cgagcggcgc
    22681 gacggcgggc ttgtccatgc caggaaagcg tttttccagg ccgttcagcg tgataacggc
    22741 atcattcatg tcctgcctcg ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg
    22801 catcatcggc gtcggtcacc acaatacgca ggcgatagac gaggtcggta cgcagatccg
    22861 gtgtttcgac ggttttcggg gtaaattcag cagtcggcga aacgaaacca atctgcccgt
    22921 gatacggctt gtccgggcga ccatcggtat aaagcagcac tttgcgcccc ggctgggcct
    22981 ggtcaagata ccgttcatca acataagcgc gcacccacac cggacgcgtt agtgaaacgg
    23041 taaacaccgt gccaccttca ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg
    23101 tgccatcaga cggggctatc aacgttgagt cctgtaaatt caactccgcc tgcgccagtt
    23161 gcgcctgcgc ctgttcgagg ctggctttcg cctgggcgat gtcctgttca cggttaccgg
    23221 aacggtactg acgcaattta tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg
    23281 aggagcgggc attttccagg tcatttgccg aaatagtgcg gcttttccac agtccttgct
    23341 ggcggttata gaagttctgc gcatagtcat aggcggcttg cgcctgtttc accgctgcgg
    23401 cggcctgagc gatttcttca tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg
    23461 ccaccgagac gcccgctttc gcctgcatca gggcaatctc atacggcttg tgatccagtt
    23521 cgcccagcac ctggcccgct ttgatagcat caccttcgtc caccgccagc gattcaacgc
    23581 gccccccaac acggaaacta agattcaccg tacgaatatc cacgttgcca tacagcgtca
    23641 ggccgttatc ctggcggctt tgataccacc agtagcctcc ggcaaccacg gcggcaagta
    23701 ccactaccgc cagttcgatc acgacaggtt ttttcatcac tacaaactcc tttgcgataa
    23761 tccttgcaaa atcaggtcga tatgacaggt caccgtctgg ttgatcagtt cggtcttttc
    23821 ttcatcgaac gcgatccagc cggtacgtaa cagaattgtt tctttgccaa gacggaacgc
    23881 cagaatctca ccaatcaacg catgggtatg aaggatcatg cgggtgtcat tggcgtcgct
    23941 gccggtccag gcggcaatca ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc
    24001 gtgcaccagg tggtaggctg ccgtgggaga gagctgctca cgggagataa acttgctgag
    24061 gttgacggtg tcatcctggg tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag
    24121 ttcacggatg gcagcccgat caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc
    24181 atgcggacgg aactgctcgc caataaaatc ggcaatccac tgggcgcagg cgaggtacaa
    24241 atcttctttc gaaccgaagt agtaggtgat ggcagcgata ttctgcccgg cttgggcggc
    24301 tatctcgcgg gtggtggcgt tcattccata ttcaccaaac tgcgccagtg cggcagcaat
    24361 cagctgtttt ttcgcctgtt cacccttgat tgtcatggca ggattattca tcgcacagtc
    24421 cattcttaat caaatgattg attaagatta tgactccata tgggagttgt ccagtatggc
    24481 taagaatttt agctacgcca gtcacaggga taatttatgc gctgcgtcac aaaaactgct
    24541 acactccgct cgctcatgac attgtggttt ttgtcatttt ccttttcagt atctccctga
    24601 aaactacacc ggtaacggtc ggggcggttc ggagtagtca tgtctttcga ttatttgggt
    24661 ttaagccctg atatcctgcg cgccgttgcc gagcagggtt accgtgaacc cacccctatt
    24721 cagcagcagg cgatccctgc ggtgctggaa ggccgcgacc tgatggctag cgcccagacc
    24781 ggcaccggca aaacagcggg ctttacgctg ccgctgttgc aacacctgat cactcgccag
    24841 ccgcacgcca aagggcgtcg tccggtacgt gcgctcattc ttaccccgac ccgtgaactg
    24901 gcggcgcaga ttggcgaaaa cgtccgtgat tacagcaaat acctgaacat tcgttcgctg
    24961 gtggtgtttg gtggtgtgag tattaacccg cagatgatga aactgcgtgg cggcgttgat
    25021 gtgctggtgg caacgccggg acgtttgctg gacctggaac atcagaatgc agtgaagctg
    25081 gatcaggttg aaatcctcgt cctcgatgaa gctgaccgca tgctcgacat gggctttatc
    25141 cacgatatcc gtcgcgtgtt aacaaaacta cctgcgaagc gccagaacct gttattctcc
    25201 gcgaccttct ctgacgatat taaagccctg gcggaaaaac tgttgcacaa cccgctggaa
    25261 atcgaagtgg cacgccgcaa taccgcgtct gatcaggtga cccagcacgt tcactttgtc
    25321 gataagaaac gcaaacgcga attgctgtcg cacatgattg ggaaagggaa ctggcagcag
    25381 gtgctggtgt ttacccgtac caaacacggc gctaaccatc tggctgaaca gctcaataaa
    25441 gatggcatcc gtagtgcggc gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg
    25501 ctggctgatt ttaaatcggg cgatattcgt gtactggtgg caactgacat cgctgcgcgc
    25561 ggcctggata ttgaagagct gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa
    25621 gattatgtcc atcgtatcgg gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg
    25681 ctggtgtgtg ttgatgaaca caaactgctg cgtgatatcg aaaaactgct gaaaaaagag
    25741 atcccgcgca ttgcgattcc gggctatgag ccggacccgt caatcaaagc cgaaccgatc
    25801 cagaacggtc gccagcaacg tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa
    25861 cagcaaccac gccgtgggga aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa
    25921 ccgtctcgcc gcctcggcga tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg
    25981 cgtaaacctg ccgctgcgca gtaatctttt atgccgggct atgcccggca tcaggctgat
    26041 gaacaaacgc aaaactgcct gatgcgctac gcttatcagg cctacgtgaa ctctgcaata
    26101 tattgaattt gcatgctttt gtaggccgga taaggcgttt acgccgcatc cggcatttca
    26161 caacaagcac ttgtcagcaa tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca
    26221 taagccaccg agacgttact tctctatagc caattgttct cgcaactcca ttaaaaggta
    26281 gcccagtcga ttcttgccct taccattacc accgtctccc cagtaagcat cgttttccgt
    26341 atgctcaacc agttttgcgg gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg
    26401 ttcgaattta gcacgaagtg ctttacgcat cacctgttct ttaaccgact cccagttttt
    26461 acgaagtggc ttggaacgat tacgccccat gcgagcggcg accatggggg aagaaacccg
    26521 acggatctct tcgcggtatt tttcatcaag gaatttttgt gcctgaaaat agtgttcgga
    26581 ggtgggccag gttttcccgt caaccttgat gggccaggcg gcaaagttag aaaaatcacc
    26641 gtagtcatcg ctggtgctgt agaaatttat gatggtgtct tgcatgacgt gttggatcct
    26701 ttgtgctcga acgggcatta aaccgcatta tgttggtggt tattgcgagc cgctttccag
    26761 aaacagaaaa accattaccc ctgaaaaccg aaaaatgcca caatattggc tgtttataca
    26821 gtatttcagg ttttctcatg gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt
    26881 ataaggcgct tcaggaacag atccccgact ttattccccg tgtgccgcag cggcagatga
    26941 ttgcggacgt cgccaaaacg ctggccggag aagaaggccg gcatctggcg attgaagccc
    27001 ccaccggcgt tgggaaaacg ctctcctatt tgatccccgg catcgccatt gcccgcgaag
    27061 agcaaaaaac gctggtggtg agtaccgcca acgtggcgtt gcaggatcag atttacagca
    27121 aagatttacc gctgctgaaa aagatcattc ccgatcttaa attcactgcc gctttcgggc
    27181 gtgggcgcta cgtttgtccg cgtaatctga cggcgctcgc cagcactgaa cccacgcaac
    27241 aggatctgct ggcgtttctt gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac
    27301 gttgtgcgaa gctgaagggc gatctcgaca cttatagatg ggatggtctg cgcgatcata
    27361 cggatatcgt tatagatgat gatctctggc gtcgtttgag taccgacaaa gccagctgcc
    27421 tcaaccgtaa ctgttactac tatcgtgaat gcccgttttt tgttgctcgt cgggagattc
    27481 aggaagcgga agtggtggtg gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag
    27541 ccgtactgcc tgacccgaaa aatctgctgc tggtgctgga cgaaggtcat cacctgccag
    27601 atgtggcccg ggatgcgctg gagatgagcg ctgaaatcac cgcgccctgg tatcgactac
    27661 agctggactt gttcacgaaa ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca
    27721 tcccaccgct ggcgatccct gaacgtttga atgcgcattg tgaagagttg tatgagctta
    27781 tcgcctcgtt aaacaacatt ctcaatctct acatgcctgc cgggcaggag gcagagcacc
    27841 gttttgcgat gggcgaactg cctgatgaag tgctggagat ctgccagcgg ctggcaaaac
    27901 tcaccgagat gctgcgtggt ctggcggagt tatttcttaa cgatttaagt gagaaaaccg
    27961 gcagccatga cattgtacgc ctgcatcggt tgattttgca gatgaaccgc gcgttgggga
    28021 tgttcgaggc gcaaagcaaa ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc
    28081 cggtgaccaa atgggcgacg cgggaagagc gcgaagggca actgcatctc tggtttcact
    28141 gcgtggggat acgtgtcagc gatcagctgg aaaggctgct gtggcgcagt attccgcaca
    28201 ttattgtcac ctccgcaacc ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga
    28261 gtggtctgaa agagaaagcg ggcgaccgtt ttgtggcgct ggattccccc tttaaccact
    28321 gcgaacaggg caaaattgtt attccccgga tgcgcgttga gccttccatc gacaacgaag
    28381 agcagcatat tgccgaaatg gcggcctttt tccgtaagca ggtggagagc aaaaaacatc
    28441 tcggtatgtt ggtactgttt gccagcggac gggcgatgca gcgctttctc gactatgtga
    28501 cggatttacg tctgatgttg ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac
    28561 tgcaccgcaa acgcgtcgcc aacggtgagc gcagcgtgct ggtgggctta cagtcatttg
    28621 ccgaagggct tgatttgaaa ggtgatctgc tcagccaggt gcatatccac aaaatcgctt
    28681 ttccgcccat cgacagcccc gtggtgatca ccgaagggga atggctgaaa agcctcaacc
    28741 gctatccgtt tgaggtgcaa agcctgccga gcgcctcgtt taacctgatt cagcaggttg
    28801 ggcgactgat tcgaagccac ggttgctggg gcgaagtggt tatctacgac aaacgcttgc
    28861 tgaccaaaaa ttatggcaag cgactactgg atgcattacc ggtatttccg atagagcaac
    28921 cggaagtccc tgaaggtata gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc
    28981 ggcgttaatg atgtgagtca ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag
    29041 agatcgggcg cgggaaaaac cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt
    29101 atgctcatat gctcaatggt gaagtccctg accttgagtt gggcggcgta ttgattgcgc
    29161 tgcgtatcaa aggagaaggg gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc
    29221 acaccatcaa gctgacgccg ccagcaggca agccgatgcc gattgtcatc cccagttaca
    29281 acggcgcgcg taaacaggcc aacctgacgc cgttgttggc gattctcctg cacaaactcg
    29341 gttttcctgt ggtggttcac ggggttagcg aagatccaac ccgcgtgctg actgaaacca
    29401 tttttgaatt gatgggtatt acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg
    29461 acgaacatca accggtgttt atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac
    29521 tggcgatgcg ctggcggatg ggcgtgcgta acagtgcaca taccctggcg aaactggcga
    29581 cgccatttgc cgaaggtgcg gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg
    29641 gacgcgtcgc gaagttcttt agcgatatcg gcgggcgggc gctactaatg catggtactg
    29701 aaggtgaagt gtatgctaat ccgcagcgct gcccgcagat caatctcatt gaccgtgaag
    29761 ggatgcgggt gctgtatgaa aaacaggaca ctgctggtag cgagttactg ccacaagcaa
    29821 aagatccgga aaccacggcg cagtggattg agcgttgcct tgctggcagc gaaccgattc
    29881 ccgaatcgct gaaaatccag atggtttgct gcctggtggc tacgggtgaa gcggcaacta
    29941 tcagcgacgg cctggcgcgc gttaatcagg cattttaatt cttttctcag ccggatgagc
    30001 catgtcatcc ggcttttacc ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc
    30061 cacgcccggc gtgcagcata aaaatacaac aaacacataa cataaacagg agttaaccat
    30121 ggaaagtggt catcgctttg atgctcagac gctgcacagt tttattcagg ctgtatttcg
    30181 tcagatgggt agcgaggaac aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa
    30241 cctggcaggg catgattcac atggtattgg catgatccca agctatgtac gctcctggag
    30301 tcaggggcac ctgcaaatta accatcatgc caaaaccgtt aaagaggcgg gggcggcggt
    30361 cacgctcgat ggcgatcgcg catttggtca ggtcgcggca catgaagcga tggcgctggg
    30421 gattgagaaa gcgcatcagc acggtattgc cgccgtggcg ctacataact cgcatcatat
    30481 cggccgtatc ggttactggg cggagcagtg tgcagcggcg gggtttgtct ctatccactt
    30541 tgttagcgtg gtcggtattc caatggtcgc gccgttccac ggtcgcgaca gccgctttgg
    30601 caccaatccg ttctgtgtgg ttttccctcg taaagataat ttcccgctgt tgcttgatta
    30661 cgccaccagc gccattgcat ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt
    30721 gccgccaggt tgcctgattg acgttaacgg cgtgccgacg accaatccgg cggtaatgca
    30781 ggagtcgccg ttgggttcgc tgttgacctt tgccgaacat aaaggctacg cccttgcagc
    30841 gatgtgtgaa attcttggcg gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt
    30901 acaaaccagt cccgatgcca ttcttaactg catgaccact atcatcatca acccggaact
    30961 cttcggcgcg ccggattgta acgcgcagac cgaagccttt gccgagtggg tgaaagcctc
    31021 gccacatgat gatgataagc cgattttgct accgggcgag tgggaagtga acacgcgtcg
    31081 cgaacggcag aagcagggga ttccactgga tgcgggaagc tggcaggcca tttgtgatgc
    31141 agcgcggcag attggtatgc cggaagagac gttgcaggct ttctgtcagc agttagccag
    31201 ctaaaaaaaa gcccgtccag tggcggacgg gcaaacaagg gtaacatagg atcaatgagg
    31261 gttagagcat atgcgtctgt cggcaaacag acagggaaat acttgtgctg gacgtagcgt
    31321 aaacgcctga tccgtccaac ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg
    31381 catcaggcat ctgttgccgg atgcggcgta aacgccttat ccgtcctacg aattacttat
    31441 aaattaccgc agtaccgctt aatttattgt tgttggtggc ggaagtgatg ctgtatccac
    31501 tggcaccggc tgccgcggct ttctcagcca gtttcgcttc cagggcatcg agggtggatg
    31561 cgccatcggc agaaaccacg ccgattttat tcatgttctg tgcctgggat gccgttatcg
    31621 gttccgccgc gaacacgcca aatgacagag ttgaaagagc catagcagca acaacagtat
    31681 tgatagtttt cataattaat ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc
    31741 gatgtgatga gtatcacgtt tttttacgag agataaaatc gaagagaatt gaccgccttg
    31801 ttcaaataaa ttgattgata tctaacatac tgataaacca tttatttgtt gtacttatga
    31861 acttgtttta taccgcgtgg cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa
    31921 taaaaattat actcgctatg catctcgcca gttgtagtta acgtaactgg ctgtctttcg
    31981 aacctctgcg attatatcct gtataagctg gtttttgtaa atctttctcc tgctgacaat
    32041 gaatacataa gcgcacgcca ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc
    32101 actcttcaca ttcatccagg ctttcgccgc gcggaatttc accccgagcg cgggcaatcg
    32161 catcttcaat tgtactgttg atctgttcgt tgacggcgtc atcgttagcc caaccggatg
    32221 ccatatcgac ctccccatat caatacttgt acagttaagt gtagctaatc cagggacgaa
    32281 ctcgggcagt tcaagcatca gatctccgac cattcccgca gcagattatg ataaagatta
    32341 agcagcgaca ggatctcttc actttcgccg tagcggcttt tcagcgactg aatattgttg
    32401 tccagttcaa acagcatggc gcgcttttta tcatcgcgga tcatcgactg gatccacata
    32461 aatgatgcca ctcgtacgcc gcgggttacg ggtggcacgc aatgctggct gctggaggga
    32521 tacaacacga gatcgcctgc cgggagtttt acccgatgtt gtccgaaggt gtcattaacg
    32581 accagttcgc cgccgtcgta gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca
    32641 gtacgcatcc agccgttttg tggatggctg cgtactgcgc catccacatg gaaaccatag
    32701 gtttcattgt tctgatagcg attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg
    32761 gcaaagaaca aagcatgttg gttaaccgcg ttcagcacct cattttgcag ggcggcgtat
    32821 aacgtgctgc gggtgtcgac ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg
    32881 gtgacgcgtc catccaccca ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc
    32941 tgtggcgata acacgccggg aatgtggtac atcatacaag ttctccaaaa agtggggcct
    33001 gcgccccaca tctgaatcag aaatgcatat tggctgtgag caagaaggtt cttggctcgc
    33061 ccgggtgata acggtagccg ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt
    33121 tgtagacgtt tagctggaag tcgagattgc gattaacccg ataccccagt ttggcatcgg
    33181 cgacccagta accttcggta aatgctggcg ttcccaccgc gccgtctgaa cctttatgca
    33241 tactgccgat atagcgtgcg cccgcgccaa cagagatatc gtcggttgcc tgatattggc
    33301 tccataaggt gaaggcgtgc tccggggtat acggcagcga tgaggaacca tcctgggcaa
    33361 catctttgcc gtttttgatg gttgcttttt gctgggtata gccgccaatc acctgccacg
    33421 cgggagtgat attcccggcc acggatatct catagccttc gacgcgtttc ttaccgtatt
    33481 gcgagtaagt tccgtcatca ttttgctcaa cttcattttc gatatcagta cggaacagcg
    33541 cggcggtgag caacagacgt ttatccagaa cctgccattt ggtgccaatc tcgctggtgt
    33601 tggctttttg cggtttaaaa tcggtgcggt tggcactgtt accgctgcca gactgcgcaa
    33661 gggcgaagtt gttgccgccc ggaggctgct gggaaacggc atagttaata tagacattgc
    33721 cgttttccgt caggtgatac agcgccccgg ctttccagtt catcagattg cccgacttgg
    33781 cggtgtcgac ggtggtgacc ggagaacctt ttgccacacc agttgggcag gtgatggcac
    33841 cgcgtccgct gccgccgcag gcggtggcac tgtcatattc agtatgataa ttatccagac
    33901 ggatcccgcc gttcagctca aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg
    33961 cgaaggtatc cgtctgacca ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa
    34021 tgctgctgtc aggatgataa atatttaccg cgggtaacgt caccggatta acgccgtagt
    34081 tagtctgcgt ttcacgggta aattccacgc cggtactgac atcatgaccg atagaaccgg
    34141 tatagaacgt cgaggtcagg ttggtctggt tggtgagaat tttattactc acatctttgg
    34201 tattcgccgt gcgtgaccag gttcagctat tcacatcgct ggtgggctga gtaatattcg
    34261 acgccccgcc cataatcgcc gtcatcaggt aatcctgctt tacgcgcgac caacgggtag
    34321 tattgcgaat ggtggtgtta tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg
    34381 tggtcgaatc gtcgtaatcg gaatccgtgc cgtaaaagtt atgagtatca acttttccgg
    34441 aatgattcag ggccgccgtt cccgcagatg gggcagaata gcccggcaaa ccgatggtcg
    34501 gaatgccgcc gtctggcgtg ttgtgctggg tgacatgcag ataattaaga tacaaacgat
    34561 tcgctgtacc aaggccaaaa gcgacagaag gggcgacgcc gtaacgctca tttttgactt
    34621 tgtcgcgtcc ggcatcgtgc gttttttcgc ccattacatt caggcgcacc gcggtggtat
    34681 caccaatgac ctgattgacg tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg
    34741 cggaggcgtc aatgccggaa tcattgcgcg gctgtttgct gatcatattg atcgagcccg
    34801 tcggtgcgct gcgcccgtag tcggtgccgg acggcccttt aatcacttcg acctgctcgg
    34861 tattgaaggt gtcgcgcgag acgctgccga tatcgcgaat gccatcaata taaatactgt
    34921 tagaggtatc ggcaccacgc atataaatgg cgtcgccagt ggtggagtta ccgttctcac
    34981 ccgcaaaaaa cgcacccacg ccg
//
LOCUS       DAJTYZ010000032        33999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000032 DAJTYZ010000000
VERSION     DAJTYZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.032"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            12..514
                     /locus_tag="OO596_001483"
                     /pseudo
     CDS             12..514
                     /locus_tag="OO596_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="OO596_001484"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="OO596_001484"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6717374.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="OO596_001485"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="OO596_001485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6717375.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="OO596_001486"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="OO596_001486"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6717376.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="OO596_001487"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="OO596_001487"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6717377.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="OO596_001488"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="OO596_001488"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6717378.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="OO596_001489"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="OO596_001489"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6717379.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="OO596_001490"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="OO596_001490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6717380.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="OO596_001491"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="OO596_001491"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6717381.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="OO596_001492"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="OO596_001492"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6717382.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="OO596_001493"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="OO596_001493"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6717383.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="OO596_001494"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="OO596_001494"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6717384.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="OO596_001495"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="OO596_001495"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6717385.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="OO596_001496"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="OO596_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6717386.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="OO596_001497"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="OO596_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6717387.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18087..19304
                     /gene="alaA"
                     /locus_tag="OO596_001498"
     CDS             18087..19304
                     /gene="alaA"
                     /locus_tag="OO596_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6717388.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19388..19987
                     /gene="yfbR"
                     /locus_tag="OO596_001499"
     CDS             19388..19987
                     /gene="yfbR"
                     /locus_tag="OO596_001499"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6717389.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMLAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20046..21878)
                     /locus_tag="OO596_001500"
     CDS             complement(20046..21878)
                     /locus_tag="OO596_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6717390.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="OO596_001501"
                     /pseudo
     CDS             complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="OO596_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22624..23118)
                     /locus_tag="OO596_001502"
     CDS             complement(22624..23118)
                     /locus_tag="OO596_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6717391.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23201..23656)
                     /locus_tag="OO596_001503"
     CDS             complement(23201..23656)
                     /locus_tag="OO596_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6717392.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23994..25196
                     /gene="ackA"
                     /locus_tag="OO596_001504"
     CDS             23994..25196
                     /gene="ackA"
                     /locus_tag="OO596_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6717393.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25271..27415
                     /gene="pta"
                     /locus_tag="OO596_001505"
     CDS             25271..27415
                     /gene="pta"
                     /locus_tag="OO596_001505"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6717394.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27605..29125
                     /gene="yfcC"
                     /locus_tag="OO596_001506"
     CDS             27605..29125
                     /gene="yfcC"
                     /locus_tag="OO596_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6717395.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="OO596_001507"
     CDS             complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="OO596_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6717396.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="OO596_001508"
     CDS             complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="OO596_001508"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6717397.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="OO596_001509"
     CDS             complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="OO596_001509"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6717398.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31145..31792
                     /gene="yfcG"
                     /locus_tag="OO596_001510"
     CDS             31145..31792
                     /gene="yfcG"
                     /locus_tag="OO596_001510"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6717399.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31849..32211
                     /gene="folX"
                     /locus_tag="OO596_001511"
     CDS             31849..32211
                     /gene="folX"
                     /locus_tag="OO596_001511"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6717400.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32232..33125
                     /locus_tag="OO596_001512"
     CDS             32232..33125
                     /locus_tag="OO596_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6717401.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="OO596_001513"
     CDS             complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="OO596_001513"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6717402.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8479 a         9279 c         8563 g         7678 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agtacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctggtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 gtcattgcga attatctgat gcaaagttat gttaggcaaa gtaatcttct attattaata
    17641 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
    17701 aattccgctt aacaatagtt tcacaccttg cccccattgc gacacctcgg aaatcatcac
    17761 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
    17821 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatc
    17881 agaacaattc actatataac attgcatgca aagcatatac gcctcattat tttgtcatta
    17941 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
    18001 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
    18061 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
    18121 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
    18181 gtaacaaggt actgaaactg aacatcggca acccagcccc attcggtttt gacgcgccag
    18241 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
    18301 aagggcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
    18361 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
    18421 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactct
    18481 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
    18541 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
    18601 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
    18661 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
    18721 aaattctcta cgacgatgct gagcatcact caattgcgcc actggcacct gacctgctga
    18781 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
    18841 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
    18901 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
    18961 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
    19021 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
    19081 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
    19141 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
    19201 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
    19261 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
    19321 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
    19381 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
    19441 ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
    19501 gatgctcgcc catgcgctgg cagctatcaa aaatcgaaaa tttggcggta atgtcaacgc
    19561 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
    19621 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
    19681 aatcgctcag caaaaactgg tcaatatggt tccggaagag ctgcgggata tctttgcgcc
    19741 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
    19801 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
    19861 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
    19921 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
    19981 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
    20041 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
    20101 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
    20161 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
    20221 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
    20281 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
    20341 aacaaacacc ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
    20401 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
    20461 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
    20521 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
    20581 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
    20641 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
    20701 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
    20761 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
    20821 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
    20881 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
    20941 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
    21001 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
    21061 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
    21121 tcacgtaatt tgagatcatc cagccgttga ccaatcattg gcgatccggg gcgaatagcc
    21181 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
    21241 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
    21301 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
    21361 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
    21421 accagcgtca tcatgccgct tatcagcccg gcaaaactca acggcatcat cagacgtgac
    21481 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
    21541 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
    21601 ttgatttcac tgttgcccgc aactttgacc agccatgttc ccattacggt ggcaacaccg
    21661 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
    21721 gaaaagccgg aaaatacttc ggggactgtc agcgttccgc ttaatgcaaa cgcgacaata
    21781 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac aatggcaacg
    21841 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
    21901 gagatgtctt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
    21961 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
    22021 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
    22081 tgacatgaca acccgccgcc aggccagaaa gcacgccagc ggagcatctt ccaccaccac
    22141 acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat caggttctgg
    22201 ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa gcccagctat
    22261 tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc
    22321 tgctttattc aaatgactga gtaaggcgat tgcccccgga agcgggtaat accttcggtt
    22381 tccgtggcct cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
    22441 cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
    22501 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
    22561 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
    22621 tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
    22681 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
    22741 accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
    22801 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
    22861 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
    22921 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa
    22981 ttcacgcatc tgtaatccgt aacctcgctc aataattgtt tgcaggcgac ggtaacgttc
    23041 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
    23101 cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag
    23161 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
    23221 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
    23281 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
    23341 accagttaag gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc
    23401 cctgcatggg taaacttaag gcgaacagtg cagtggcaac tgccggccca agctgaccgc
    23461 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
    23521 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagtg
    23581 gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat
    23641 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
    23701 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
    23761 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
    23821 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
    23881 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
    23941 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
    24001 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
    24061 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cctgaagcac
    24121 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
    24181 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
    24241 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
    24301 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
    24361 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
    24421 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
    24481 tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
    24541 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
    24601 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
    24661 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
    24721 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
    24781 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
    24841 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
    24901 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
    24961 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
    25021 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
    25081 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
    25141 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
    25201 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
    25261 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
    25321 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
    25381 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
    25441 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
    25501 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
    25561 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
    25621 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
    25681 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
    25741 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
    25801 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
    25861 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc ctctgccggt
    25921 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
    25981 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
    26041 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
    26101 ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg
    26161 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
    26221 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
    26281 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
    26341 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
    26401 gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac
    26461 tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac
    26521 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
    26581 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga
    26641 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa
    26701 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
    26761 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
    26821 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
    26881 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
    26941 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
    27001 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
    27061 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
    27121 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
    27181 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgtttatct tcccggatct
    27241 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
    27301 gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
    27361 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaacctcg
    27421 tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
    27481 ttttattctc ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
    27541 atgaattgcg ccatttcact ttacatactt accactttgt tttgtgcaag ggaatatttg
    27601 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
    27661 ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg
    27721 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    27781 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    27841 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
    27901 attaacctca ggatcgaaat acgggacagc tgttggcatc atcatgttta tgctggtgat
    27961 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    28021 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
    28081 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
    28141 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc
    28201 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg
    28261 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    28321 cactatgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    28381 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    28441 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
    28501 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    28561 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    28621 taataccatg gcttcatcct ttaccgaagg gacgcgaatg atgatcgccc ctgccctgct
    28681 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
    28741 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
    28801 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc
    28861 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
    28921 ccgtcaggtt accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc
    28981 aacctcagct tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct
    29041 gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg
    29101 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    29161 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    29221 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    29281 ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct
    29341 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    29401 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    29461 ttgctcatcg gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    29521 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    29581 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    29641 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    29701 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    29761 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    29821 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
    29881 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    29941 caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg
    30001 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    30061 atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg
    30121 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    30181 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc
    30241 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    30301 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    30361 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    30421 actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    30481 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    30541 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    30601 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    30661 ggatgggcat cagatcgctg cgcagccagg cctgaatccg tcgtgcacgc gcacgatttt
    30721 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    30781 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    30841 gtgtctgacc gtaaccttgc cacgtcggct tcaaatgttc accgctgtcg aggtcgatgg
    30901 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    30961 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    31021 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    31081 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc
    31141 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    31201 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    31261 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    31321 tcctgccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    31381 gactgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    31441 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    31501 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    31561 tctttaccat gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    31621 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    31681 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    31741 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    31801 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    31861 gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa
    31921 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    31981 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    32041 attcagcatg tagagaataa ccgtttctct ttgttggaaa aattaactca ggatgtgctc
    32101 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    32161 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat
    32221 cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga
    32281 ttccgcgttt gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag
    32341 ccagttccgt tctcggccct cgggtgacgc tatggcaagg gctagccgat caaagcaacc
    32401 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    32461 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    32521 atttgattaa tgccagcgac acgccgccgt cggtattcat ttccggctcg gcaacgggct
    32581 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    32641 ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc
    32701 gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa
    32761 tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    32821 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgacctgc
    32881 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    32941 tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt
    33001 taatgggcga atcttcaata ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    33061 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    33121 gctgatgtgg tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc
    33181 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    33241 aacctctatc cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg
    33301 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    33361 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    33421 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    33481 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    33541 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    33601 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    33661 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    33721 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    33781 tgcgaaaagc cattaattct tccagcttac tttggtgctc tatcactaca taaatataac
    33841 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    33901 catcaataaa actgtttgat tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    33961 gcgcgggaag atgaatatca ataaaatccc gcgcggtgt
//
LOCUS       DAJTYZ010000033        33996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000033 DAJTYZ010000000
VERSION     DAJTYZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.033"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..2602)
                     /gene="acrF"
                     /locus_tag="OO596_001514"
                     /pseudo
     CDS             complement(1..2602)
                     /gene="acrF"
                     /locus_tag="OO596_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2614..3771)
                     /gene="acrE"
                     /locus_tag="OO596_001515"
     CDS             complement(2614..3771)
                     /gene="acrE"
                     /locus_tag="OO596_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR6717403.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4170..4832
                     /gene="acrS"
                     /locus_tag="OO596_001516"
     CDS             4170..4832
                     /gene="acrS"
                     /locus_tag="OO596_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR6717404.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4835..5014)
                     /locus_tag="OO596_001517"
     CDS             complement(4835..5014)
                     /locus_tag="OO596_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6717405.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5098..5982)
                     /gene="yhdJ"
                     /locus_tag="OO596_001518"
     CDS             complement(5098..5982)
                     /gene="yhdJ"
                     /locus_tag="OO596_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6717406.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6068..6364)
                     /gene="fis"
                     /locus_tag="OO596_001519"
     CDS             complement(6068..6364)
                     /gene="fis"
                     /locus_tag="OO596_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6717407.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6390..7355)
                     /gene="dusB"
                     /locus_tag="OO596_001520"
     CDS             complement(6390..7355)
                     /gene="dusB"
                     /locus_tag="OO596_001520"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6717408.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7684..8565)
                     /gene="prmA"
                     /locus_tag="OO596_001521"
     CDS             complement(7684..8565)
                     /gene="prmA"
                     /locus_tag="OO596_001521"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6717409.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8577..10028)
                     /gene="panF"
                     /locus_tag="OO596_001522"
     CDS             complement(8577..10028)
                     /gene="panF"
                     /locus_tag="OO596_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6717410.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10018..10260)
                     /locus_tag="OO596_001523"
     CDS             complement(10018..10260)
                     /locus_tag="OO596_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6717411.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10369..11718)
                     /gene="accC"
                     /locus_tag="OO596_001524"
     CDS             complement(10369..11718)
                     /gene="accC"
                     /locus_tag="OO596_001524"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6717412.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11729..12199)
                     /gene="accB"
                     /locus_tag="OO596_001525"
                     /gene_synonym="fabE"
     CDS             complement(11729..12199)
                     /gene="accB"
                     /locus_tag="OO596_001525"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6717413.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13177..14151)
                     /locus_tag="OO596_001526"
     CDS             complement(13177..14151)
                     /locus_tag="OO596_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6717414.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14303..16243
                     /gene="csrD"
                     /locus_tag="OO596_001527"
     CDS             14303..16243
                     /gene="csrD"
                     /locus_tag="OO596_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6717415.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16548..17591
                     /gene="mreB"
                     /locus_tag="OO596_001528"
     CDS             16548..17591
                     /gene="mreB"
                     /locus_tag="OO596_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6717416.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17657..18760
                     /gene="mreC"
                     /locus_tag="OO596_001529"
     CDS             17657..18760
                     /gene="mreC"
                     /locus_tag="OO596_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6717417.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18760..19248
                     /gene="mreD"
                     /locus_tag="OO596_001530"
     CDS             18760..19248
                     /gene="mreD"
                     /locus_tag="OO596_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6717418.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19257..19850
                     /gene="yhdE"
                     /locus_tag="OO596_001531"
     CDS             19257..19850
                     /gene="yhdE"
                     /locus_tag="OO596_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6717419.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19840..21309
                     /gene="rng"
                     /locus_tag="OO596_001532"
     CDS             19840..21309
                     /gene="rng"
                     /locus_tag="OO596_001532"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6717420.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21377..25177
                     /gene="yhdP"
                     /locus_tag="OO596_001533"
     CDS             21377..25177
                     /gene="yhdP"
                     /locus_tag="OO596_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6717421.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25333..26778
                     /gene="tldD"
                     /locus_tag="OO596_001534"
     CDS             25333..26778
                     /gene="tldD"
                     /locus_tag="OO596_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6717422.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFRSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26906..27771)
                     /locus_tag="OO596_001535"
                     /pseudo
     CDS             complement(26906..27771)
                     /locus_tag="OO596_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27954..28157
                     /locus_tag="OO596_001536"
     CDS             27954..28157
                     /locus_tag="OO596_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6717423.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28165..29097
                     /gene="aaeA"
                     /locus_tag="OO596_001537"
     CDS             28165..29097
                     /gene="aaeA"
                     /locus_tag="OO596_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6717424.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29103..31070
                     /gene="aaeB"
                     /locus_tag="OO596_001538"
     CDS             29103..31070
                     /gene="aaeB"
                     /locus_tag="OO596_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6717425.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31162..31434
                     /locus_tag="OO596_001539"
     CDS             31162..31434
                     /locus_tag="OO596_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6717426.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31490..31753)
                     /gene="yhcN"
                     /locus_tag="OO596_001540"
     CDS             complement(31490..31753)
                     /gene="yhcN"
                     /locus_tag="OO596_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6717427.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32118..32588)
                     /gene="argR"
                     /locus_tag="OO596_001541"
     CDS             complement(32118..32588)
                     /gene="argR"
                     /locus_tag="OO596_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6717428.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33023..33961
                     /gene="mdh"
                     /locus_tag="OO596_001542"
     CDS             33023..33961
                     /gene="mdh"
                     /locus_tag="OO596_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6717429.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8253 a         8453 c         8901 g         8389 t
ORIGIN      
        1 ttcctgatac gacatacctg tccagtcata accaatgccc gcaggtaatt ttgacgcaag
       61 gttttccatc aatgccatgg catcgccgga actggttcct ggcgcggctt ccccctgaat
      121 ctccattgac ggcagaccgt tgtagcgttc cagtcgcgga gagccataca cccaatgtga
      181 agtggtaaag gcagagaatg gcaccatttc gccgttggcg ctgcggacat aaagtttatc
      241 gacatcttct ggcagcatac ggaatttggc atccgcctga acatacacct ttttcacgcg
      301 accacggtcg atgaagtcgt taacgtaagt cccacccagc gccgttgaaa tggtctgatt
      361 gatgtcagaa agtgaaacac ctaatgcctg cgccttttcc tggtcaactt ccagtttaaa
      421 ctgcgcggtg tcttccagac cattaggacg cacgctgact aagctggcag gatgttgcgc
      481 cgccatgcca agcaactggt tacgggcctg ggtcagggca tcgtgaccca gcccagcctg
      541 atcaattaac tcaaagtcga aacccgttgc cgtgcccagt tcaacaatgg ctggcatatt
      601 gaatggaatg acaaaaccgt cgcggatctt gcccaattcc attttggcac gatggattac
      661 cgcttccgca ctgttttcgt caccactacg ctcttcccac ggtttcagac tgacgaaagc
      721 cataccggcg ttttgtgcct ggccgctgaa gctaaagccg ttaaccgtaa agacactttc
      781 tacgttcgct ttctcgttat tcagatagta atccgtcact tgatccaaca ctttttgcgt
      841 ccgctcttgc gtcgcgccag cgggtaactg aatcatggtc agaaagacac cctgatcctc
      901 ttcaggtaag aaggaagaag gaagacggaa gacgtaaaaa caacaccacc attcctgcaa
      961 caatcagcgc atagatcagt aaatatcgtc ctgtggaacc gaggattttg ccgacgctgt
     1021 tggtgtagtg gttaacgcta tgatcgaagg tggtattaaa ccagccgaag aaaccgccct
     1081 tattttcgtg atgctcagca gagacgggtt taagcagcgt tgcacataac gcaggggtaa
     1141 gaatcaatgc caccagaaca gaaagcgcca ttgccgaaac gatggtgata gagaactggc
     1201 gataaattgc cccagtagaa ccgccgaaga atgccatcgg aataaatacc gctgacagca
     1261 ccatcgcgat acccaccagt gcgccctgaa tttgcgacat cgatttttcc gtcgcttctt
     1321 ttggcgggag cttatcctcc atcatcacgc gctcgacgtt ctccaccacc actatcgcat
     1381 catcgacgag cagcccgatg gcaagcacca tcccgaacat cgttagtgtg ttgatggagt
     1441 aaccaaaagc ggcgaggatg gcaaacgtcc ctaacaacac cacgggtacc gcaatggtgg
     1501 ggatcagcgt tgctcgcata ttctgcaaga acagatacat caccaggaac accagcataa
     1561 tggcttcgaa cagcgttttt accacttcgt gaatagaaag ctggacgaat ggcgtggtgt
     1621 cataagggta gagaaccttc attccctgcg ggaagaatgg ctgtaattcc gccagttttg
     1681 ccttaatggc tttcgcggta ttgagagcgt tcgcgccggt tgctagctta atccccaggc
     1741 ccgccgccgg ttttccgttg atacgagcga taacgttata gttttcaccg ccaagttcaa
     1801 ctcgtgcgac atctttcagg cgtaccaccg agccgtcact gtttacgcgc agggttactt
     1861 tgccgaattc ttccggattt tttaaccgcg tctgagcaat aatcgaggcg ttcaactgtt
     1921 gccctggtaa cgctggcgtt ccgcccaact gcccggcagc gatctgatcg ttctgtacct
     1981 tcaactggtt aatcacatca accggtgtca gtttatattt gtttagcaga tcggcgtcca
     2041 gccagatacg catcgcatac tgtgcgccga aaagctgtac gtcaccgacg ccattcagac
     2101 ggctaagcgt atctttaacg ttagaggcca catagtccga gatatcgtcc tgtgtggtgt
     2161 ctgggttatc agagacaaag cccgccacca tcaaatagct gctactggac ttttcaacac
     2221 tgatcccctg ctgctgaacc tcctgcggca gcaacggcgt ggcgagctgg agtttgttct
     2281 gcacctgcac ttgcgcgata tcaggatcgg tcccggactg gaaggtaagg gtaattgtca
     2341 cgctaccggc ggaatcgctg gtggaggaca tatacatcag gttatcgata ccattcatat
     2401 tctgttcgat aacctgcgtc accgtatcct gcacggtctg cgcatcagca cctggatagt
     2461 ttgcctaaac agaaaccgca ggcggtgcaa tggttggata ctgagcgacg ggcaattgta
     2521 ggatcgccag tgcgcccgcc atcatcagaa taatggccag cacccatgca aatatcggtc
     2581 gtcgaataaa aaagtttgcc atgtcagatt accttacttc gatgcagtat ctgcgggggt
     2641 atcggtagtg gctttaacct gctctcccgg acgcgctttt tgcaggccgc tgacaatgac
     2701 ttgatcgcca gatttcagac cttcgctaat caaccattta tcgccaatcg cctgactggc
     2761 aacgacaggg cgcgcttcca cctgactttt atcgttaaca atcagcacgg ttgcatcacc
     2821 acgcggtgtg cggctaacgc cttgttgcgg gataagaatg gcgtcaggtt ggacgccttc
     2881 atcaatccgt gcacgcacaa acatacccgg caaaagcgta tgttgcgggt tagggaagac
     2941 agcacgtagg gttatggagc cggtgcattc atcaacggtc acatcagaga attgcagcgt
     3001 acctttcagg ggataggttt gaccgttttc catgaccaac tctacgttgc tggtggcgtt
     3061 ttccttatgc aaatttcctt gctctacgga ttgcttcagc ctcataaaat cgttgctgga
     3121 ttgggtcaca tcaacgtaga taggatcgag ctgctggaca gtcgccagtt cagtcgtttg
     3181 cccattagtg acaagagcgc cttcggtcac ggtcgatttg ccgatacgtc cgctaattgg
     3241 cgcagtcact ttggtataag cgagattgat gcgagcgctt tcgactgtgg cttttgcggc
     3301 aatcacggcg gcatcggcct ggcgagcatc agcaatggcc tggtcgtact cctgctggct
     3361 gatgtatttc gtacccacga gcggaacgta acgttttacc gtcaaatgcg cgatggcggc
     3421 ggcggcttca cttttcgcca gttcgccttt cgcgctgtca taatttgcct gataggtcgc
     3481 gggatcgatc tggtacaggg actggcctgc ttgtacatcg ctgccttcag tgaaattacg
     3541 attcagtacg atcccgctaa cctgtgggcg aacttcggct atacggtaag cattggtgcg
     3601 gcctggtaat tcagtcttaa cttctaacgg ggccgttttc acaatatgaa cggtaacctg
     3661 cggttcaccg acgtgagctt tctcttctcc cttatcgtta caaccgacga ttaaagccgc
     3721 ggagatcaga ataaaggagg gcaggaggaa aaacctggca tgtttcgtca ttactattcc
     3781 tcaaaaaaac aaaagcgcgt tatttaccca gaaaggcaac gcgttaactc gcagaaagaa
     3841 aaatacagtt tgctatccta caaattatca ttcgtcgatg taaggaatag ttatgaatac
     3901 tggcgtctca aggcacataa acacaaaaaa agattaatat tctactgttt tattttgacg
     3961 cgggttgaaa gaggcagaat taaaacctcg taaattgaaa tatatattca tgtggcgaat
     4021 gtattttagg taaataatat atattattta tttgacgatt tatagcaact caatgttacc
     4081 ctctcgtata aatacacatt agatgataga ttaaccttcg ctattttctc accctgtgtc
     4141 gaatatattt atttcctgaa taattaatca tggcaaaaag aaccaaagcc gaagctctga
     4201 agacccggca agaactgatt gaaactgcca tcgcccagtt tgcgcaacat ggcgtaagca
     4261 agacgacgtt caacgacatt gccgatgccg ctaacgttac gcgtggcgct atctactggc
     4321 acttcgagaa caagacccaa ctgtttaatg agatgtggtt gcaacagcct tcattgcggg
     4381 agttaatcca ggaacacttg acggctggat tagagcatga cccgtttcaa caattgcgtg
     4441 aaaaattgat tgtcggcttg caatatattg ccaaaattcc ccgccagcag gcgttgctga
     4501 aaatcttata tcacaaatgt gaatttaatg atgggatgct ggccgaggga gtgatacgcg
     4561 aaaagatggg ctttaatccg cagactctcc gcgaagtatt gcaggcgtgt cagcaacaag
     4621 gttgtgtagc aaataacctc gatttagatg ttgtgatgat tattattgat ggtgccttca
     4681 gcggaattgt tcaaaactgg ttaatgaata tggcgggtta tgatctttat aaacaagcac
     4741 ccgcgctggt cgataacgta ttaagaatgt tcatgccaga tgaaaacata acgaaattaa
     4801 ttcatcaaac aaatgaatta agtgtcatgt aaattcacat tctgtcctga aaattcagtt
     4861 cgtcacagtt aaccagtttt agtggataaa ctgagttcat attacggcat ttgtctgttt
     4921 ctgttgtcag cagttcaatc cactgcatca gtaaagtatc aaacagaaaa acgaagacag
     4981 cgaaaacgac gagccaccaa tacttgcgaa tcattacctg agcctgtgca tgtttaacca
     5041 agtctggcag gaaatataca cgaaatgaat aatctgaaaa ccaggcttac acttccttta
     5101 ctttgcaatg agatcggggt caacttcgct taaccggctt cgttttgaca ggttgccggt
     5161 ctttcttttt ttcactttcg ccagttcttc cgcggagtaa tgcgacgcga catccagccg
     5221 tcgaagcccc attttgatgt actcgctgtt gatctcaata ccaatgaatt ttcgtccgct
     5281 ggcgatggct acggcaccgg tagtaaagct accggcaaac gggtcgagaa cgatatcgcc
     5341 tgggttggaa gaggcgagaa taatgcgttt cagtaaggct tccggttttt gcgtcgggtg
     5401 gttctcatat tcatccatca aataacgcac gcgcggaaaa tcccagacgt tacccggtac
     5461 tttttgatga ttgtatggct gtggaggatt tttgcgataa tcgatcaacg cgcgctgcga
     5521 tccggttttg gcttctacca gaatagcatc accgttgaat gtgtagttct ttgcgtcttt
     5581 caccatcatc aggatgggtt cgtacatgga gccgtagtgt tttttcgcct gtactccaga
     5641 actgtcatat gaccagacga tgcgactttt gatggtaaaa agcttgcggc actggagatc
     5701 gataaagggc atgttttccg tactgttcat aatgtacatg ctgccctgct ttttcagaac
     5761 gcggtggcac tctacaatca cttcaaacag ccagtcgata aacagatctt ctttccaggc
     5821 ttcgatcaga ccatcaaaat ttttaccgat gttatacggt gggtcggcaa agatcagatc
     5881 gacgctttca gtaggtagct ttttaagttc ggcaagggca tcaccgtgaa taatggtctt
     5941 agcttcatta ccaaaccggg tcggttcaca tcctgttctc atggtcactc cctttgtgac
     6001 acctataaaa aaggcgcttc cccatgccga gtagcgcctt tttaatcaag catttagcta
     6061 acctgaatta gttcatgccg tattttttca attttttacg cagcgtacca cggttgatgc
     6121 ccatcatcag cgcagcacgg gtctggttac cacgggtgta ttgcatcacc atgtccaaca
     6181 ggggctgttc tacttcagcc agtaccagct catagaggtc attcacatcc tgaccattca
     6241 gttgagcaaa atagttcttc agtgcctgtt taaccgagtc acgcaggggt ttttgggtta
     6301 cctgatcctg agagttaacg gtagaaacgg tcagtacgtc agaatttacg cgttgttcga
     6361 acatagttct gtcagctctt tatttctgtt tacgcaaaat tttcgaagta tgcctccaac
     6421 gcctccagct gttcgctggc atcctcaatg gcgttgaatg tgcgccgaaa ctggtcattt
     6481 ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg caattcggta cccttttgcc
     6541 ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa gcaagcgctt aacctctgcc
     6601 aaaggcagcg ggggcagcaa ctccccagtg tccagataat gctggatttc ccgaaagatc
     6661 cagggtcttc cctgagctgc gcggcctatc atcagggcat ccgcccctgt atagtcgagc
     6721 acagctctgg ctttaagcgg gtcagtaatg tcgccattcg cgataaccgg aatggaaact
     6781 ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc cattgaacaa acaggcgcgt
     6841 gtacggccat gaatggtcag agcctgaatg ccacagtctt cagccagttg ggcaatctct
     6901 tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct tcagggtaac aggaacgtcc
     6961 actgcattga cgacctcggt aaggatcgat ttaacgacat ccgggtactg caagagggct
     7021 gaacctgcga gcttgcgatt cactttttta gccgggcaac ccatattgat atcaataatc
     7081 tgggcaccgc tttccacgtt aatacgtgct gcatctgcca tttctttcgg atcgctacca
     7141 gcaatttgca cggtgcgaat accgggttca tcaatgtgca ccatccgtaa acgagatttg
     7201 tcgctttccc aaacctgtgg gttagaagac atcatctcgg atactgtcaa tccggctccc
     7261 atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc cagccatggg cgctgcgatc
     7321 aggcgatttc tgagctgata ttgtccgatg cgcatgagtt aagaaatgac catactgtga
     7381 ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg aaaggccaaa ctttgaccaa
     7441 tcctctgaga tggatcaaag aattgcattt aaaatgagcg tggtgagata attactcata
     7501 aaaatcatca tattagaaaa tagtgactaa aatttacact caaagaaatt tgagtaagtt
     7561 ctcaattttt ctttatgaat gaaaattttg gcacgcaaat ttgcgtaaat aaccggcaaa
     7621 tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc gtcaccctac ggcgatgcga
     7681 aggttaattc ttacgaccgg taatgcggca ccactcttct ttttccacga ccgggtccag
     7741 tgcgaagctg tcgacataag cttcacaaac gctctctgcc tggcttgcca gaataccgga
     7801 aaggcccagc aaaccgcctg aaaccggcag gacgctgatt aacggtgcca gttcacgtaa
     7861 tgggcctgca aggatgttag cgaccaccac gtcggctttc atttcttctg gctgatcttt
     7921 cggtaagtag agttccagac ggtcagaaac gccattacgt tcggcgttgt cgcggctagc
     7981 ctgaatcgcc tgcggatcga tatcaatacc aatggctttt gctgcaccca gtttcagcgc
     8041 cgcgatagcc agaatgccgg aaccacagcc aaagtcgatg actattttac cggttaaatc
     8101 gaggctgtcg agccactgca ggcacagaga ggtagttgga tgggtacccg taccaaacgc
     8161 cagccctgga tctaacatca cgttgacggc gttttcgtcc ggcacatcac gccagctagg
     8221 gcagatccac agtcgttcac caaagcgcat cgggtggaaa ttatccatcc attcgcgctc
     8281 ccagtcttta tcttccagtt gttcgatttt atgcgcgaag cctgcgccga gcagcggatg
     8341 gttttccaga atcgccacca cgtcgttcat atcggtttca gcgtcgaaca gaccaatcac
     8401 atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc tcaaatactg gcgtatcgtg
     8461 ggtatcctga aaagtgatag aaacggcacc cgcttccatc agcgcatcgc taagatcttc
     8521 agcgttcgcg ccggtggtgt tcagtttcag ttggatccaa ggcatggcaa aactctttat
     8581 ttatcagtag tcaaaacggt agcttgcggg acggatgtac cgaaacggtt tccgaccagg
     8641 aaagccagca aacttagtag taacgagggc acgatagggt ggaagcccag gtactgaata
     8701 ttcagcgtcg cgagtacggc atacagcacg ccgccaacga tcatcgcact tagcgcgcct
     8761 ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg gccacaggaa aacggcttcc
     8821 agcccaccga aggccagcaa attcagccag atgatcattt ctggcggctt ccaggcggca
     8881 agcagcagca acgcgccgag aactaacgta attaccgccg acatccgctt cagacgcgtc
     8941 tcgttttgca tttgatccgg acggatattc agatagagat ctttaatgat cgtagcggaa
     9001 ctttgtagca attgggcgtt aattgtcgac atgatcgcag ccataggtgc agccagaaag
     9061 atcccggcag caaacggtgg cagcactttt accattaacg tcgggatcac cagatccggt
     9121 acggtgagat cggggatcac tgcacgacct aacgctccgg ctaggtgcat accgaacatc
     9181 aggattgcga ctacaatcgt accgatgatg atcccccggt gtaccgcttt gctgtcttta
     9241 taagagatac agcgcaccgc agtatgcggc aggccaatca cgccaaagca caccagtacc
     9301 cagaacgacg tcataaaggc aggcgacaga atatcgtcag cgccttgtgg cgtaaccagt
     9361 tgcggatcga tggtttgcaa ggtctgtact gcgttgctta agccgccagc ggcatgtacc
     9421 acgccaataa gcagcacaac ggtgccaatc agcatcacaa gcccttgcat ggtgtcgttc
     9481 agcacgctgg cgcgaaagcc gccaaaggcg gtatacaacg caatgctgat accgaaaatc
     9541 agcagcccgg tttcataagg aatacccgcc gcggtttcca gcaggcgcgc accgccgata
     9601 aactgcacgg tcattgcccc aacgaacgca accagcaaac tcaaactcgc cagccacacc
     9661 agaagacgac tctggtagcg ggcaaacagc atgtcgttca gcgtcactgc attgtagcgg
     9721 cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg aaagccagac tgcaggaagc
     9781 tgaatcatcg ccagcaatac ccagcccagc ccgtatttat aagcagctcc tggcccgccg
     9841 ataaacgaac tggcactgat ataggtcgcg gtgagcgtca tcgccagcac aataccgccc
     9901 atagagcggc tgccgaggaa atactcatta aggaaggtgc cggtgctccg tttacgcatc
     9961 gcataaaccg agataccgaa caccaccacc agataggcga ccagcggtag aattacttca
    10021 agctgcatcg tcatcctcca gtgggatatc gcgatagata aatttcacca tcgcccagca
    10081 cagtccaata aacagtagcg gcgtcaggat gcaggccatc tcaaaccagc gcggaaagcc
    10141 ggtaaaaccg ggggcaacgc caggtaagta agcggctact aaccaaactg ccagatacaa
    10201 aagggtcagc cccagcgccc agcgcgcctc tttatgggcc tgaacaaaac gagtgtccat
    10261 tttttgtccc tgatgggtga agaaagcgag gattgtacct tatgggggtt gtcgatcccc
    10321 agtaataaaa aaggccggaa aatccggcct tttggcgctt ttgcagtctt atttttcctg
    10381 aagaccgagt tttttctcca gatagtggat gttagtgcca ccatgctgga agttctcgtc
    10441 attcatgatg cggatctgca gatcaacgtt ggttttgata ccgtcgatga tcagctcctg
    10501 cagcgcattc ttcatgcggg caatcgccac gtcacggttt tcaccgtagc aaatcagctt
    10561 accgatcatt gagtcatagt acggcggtac ggtgtagccc gcgtagatat gagactccca
    10621 acgtacgcca aaaccgccag gtgcgtggaa acgggtgatt ttgcctgggc ttggcaggaa
    10681 ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca tggccgcgaa cgtgaacttc
    10741 ttcttgcttg atcgacagcg gttgaccggc agcgatacgg agctgttctt tgatcaggtc
    10801 aacgccagtg atcatttcgg taaccgggtg ttctacctga atacgggtgt tcatttcgat
    10861 gaaatagaac tcgccgtttt cgaacagaaa ctcgaaagta cctgcaccgc gatagccgat
    10921 atcaacacac gctttagcgc aacgttcgcc gatgtagcga cgcagttccg gggtaatgcc
    10981 cggtgctggc gcttcttcga ccactttctg gtggcggcgc tgcatggagc agtcacgttc
    11041 cgccagatag atagcgttgc cccgaccgtc agccagtacc tgaatctcga cgtggcgagg
    11101 attttccagg tatttctcca tgtaaaccat atcgttgctg aaagcagctt tcgcttccgc
    11161 acgggtcatg gagatggatt gtgccagttc agcgtcgccg cgcactacgc gcataccgcg
    11221 accaccgccg ccgccggagg ctttgataat caccggataa ccaatgcgtt tagcaatggc
    11281 acggttttta tccatatcgt cgcccaacgg gccgtcagaa cccggtacgc aagggacgcc
    11341 cgcttttttc atcgccgcga ttgcggatac tttgtcgccc atcaggcgaa tggtttctgc
    11401 tttcgggccg atgaagataa agccggagcg ttcaacctgc tcggcaaagt tggcgttctc
    11461 ggagaggaag ccgtaacccg gatggattgc tactgcgccg gtgatttcag cggcgctgat
    11521 gattgccggg atgttcagat aactttttac tgacggagca gggccaatac agaccgtttc
    11581 atctgccagt aatacgtgtt ttagatcgcg atccgcgctg gagtgcacag cgacagtctt
    11641 gatgcccagt tctttacagg cacgaagaat acgcaatgca atctcgccgc ggttggcaat
    11701 aacaatttta tccagcatgt tcgcctcgtt actcgatgac gaccagcggc tcgtcaaatt
    11761 ctaccggttg tccactttcg accagaattg ctttcacggt accggatttg tccgcttcga
    11821 tctggttcat cattttcatg gcttcaacga tgcacagggt atcgcccacg ttgactttct
    11881 gacccacttc gatgaacgct tttgcgtccg ggcttggggt gcggtagaaa gtaccaacca
    11941 tcggggaacg tacgatgtga ccactgattt ccgctgctgc tggcgcttcc atggaaggaa
    12001 cggtcgccgg agcggctgcg ttagattgag ctggctgctg catcattggt gcagcgtaag
    12061 cttgttgcat cacagggaaa cttgcggcag gagctgcacg gctaatgcgt actgactctt
    12121 cgccttcaga aatttccagt tcggagatgc ctgattcttc aaccagctcg atcagttttt
    12181 taatcttacg aatatccatg agtgggttcc gtactctttg tttagtgtga ttgtgacagg
    12241 cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg tatgcatagc gataaattcc
    12301 agcaggccgg gacgccggtc tattttgccc cagagtatca atgttagact tgacggacat
    12361 tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa tataccttcg ttgcgccaat
    12421 gtcacctttt ccgcagcgca aaaactgcgt cagggaggac gaggtcgcac attataacga
    12481 tttcgtagca attggcagct aaatactggt ctcatcaggg aagataatca acagctaaca
    12541 tgtaaataac cttcaacacc gtgtaatttg caacaagccg cacaattcac gaaattagcg
    12601 ccatcatcga cggaacttct tataacgtaa ggtgaaaagc tgtaaagcca gccctgcgaa
    12661 gatgaggggc tgcaatgaga taatcttcac agaccacaaa taatgtaggg atgccaggat
    12721 cgcgacaaga tagacgacat tacgcagcgg ttgctgggga ttacccaatt ttcactgcat
    12781 cgtctggata gatgtgaatg ctaaagcacg caaaatcaac cgactgacaa ttctcagcat
    12841 taaataggtt cgagtggtta atttgtagtc aaataaaatc agattattca ccccatggta
    12901 ccagaaatca gtaacaggtt aagcgtagtg tcgcccaggc aaagcaccat aaacctaaca
    12961 ggcgacggag gcgtatcaat aatggctgtt tagaggagta tcaatgcgca tcaatttgtg
    13021 gtcagtcgaa atcatgactt tccagtttgg atgatgttgt acaacttcct gaccaaggtg
    13081 ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac cgattgaata atgatgtcaa
    13141 aatgagttcc ctctcttatt attccttgct aaatggttag ttaaccttca ccagcgtgcg
    13201 accctggatc tggttattaa tgatggcctc ggcaaagttc ggtgcctctg acagagatat
    13261 ctcttttgcc gcctgggtat agaatgattc cggtaaatcg gcgaccagtc gctgccaggc
    13321 ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc ccttgcaaac ggacattacg
    13381 cagaataaat ggcatgaccg tggttggcag agtaaaacca cccgccagac cacaggccgc
    13441 cacgcagccg ccgtaattca tttgcgccag cacttttgcc agcactttgt cgccaacagt
    13501 gtcaattgcc ccagcccaga cttgtttttc cagaggacgg gattcggcaa actcatcacg
    13561 agggagaata cggctggcac ctaaactttt cagatattca tgggtacttt cgcgaccgga
    13621 aacggcaacg acctgataac ccaacttatg cagcagcgcc acggcggtac tgccgacgcc
    13681 accactggca cccgtcacga caatctcccc gtcctgcggg cgaacaccgg catcttccag
    13741 cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg ataatcattg ctttacgcgc
    13801 gtccagccct tgcggcatgg caaccagcca gtcacctttc actcgcgcct gctccgccag
    13861 cccaccccag tggttttcac caacgcccca gccagtgagt aacacctcct gaccggcatg
    13921 aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa tcgatcccag gaatcatcgg
    13981 aaaattacgg atgatttttc ccttaccggt aatcgccagc gcatctttat agttcaggct
    14041 cgaccagtga acatcgaccg tgacatcgcc ctccggcagg cgactttcgt ccagagtctg
    14101 tactgatgcg agagttttgc cgtcctgctg ttctaaaagt aacgcctgca taagtggtcc
    14161 tcatgtgcat gatggattgg aaaataattt ctgaagatta tactcgctaa tggaaaagca
    14221 aagccgatga agcgcaataa attgcgagat aaatctgatt tgctagtatg cccgcttcct
    14281 cactatcgga gttaacacaa ggatgagatt aacgacgaaa ttttcggcct ttgttacgct
    14341 gctcaccggg ttaacaattt ttgtgacttt gctgggctgt tcgctaagtt tctacaacgc
    14401 aattcagtat aagtttagtc atcgcgttca ggcggtggcg acggcgattg atacccacct
    14461 tgtgtcgaat gacttcagca cattaaggcc acaaattacc gaattaatga tgtcggcaga
    14521 tatcgttcgt gtagacctgc tccatggtga taaacaggtt tataccctgg ccagaaatgg
    14581 tagttatcgt ccggttggct ccagcgatct gtttcgcgaa ctgagtgttc cgttgataaa
    14641 gcatccgggg atgtcgctgc gtctggttta tcaggatccg atgggcaact atttccattc
    14701 tttaatgact accgcgccgc tcacgggggc gattggcttt atcattgtta tgctcttcct
    14761 ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa ttgctggaaa cccgggctac
    14821 tcgtatctta aacggtgagc gtggctctaa tgtgttggga accatctatg aatggccgcc
    14881 cagaaccagc agtgcgctag atacgctgct tcgtgaaatt cagaacgcac gcgaacaaca
    14941 cagccgtctt gatacgctga tccgctctta tgccgcccag gacgtgaaaa ccggcctcaa
    15001 taaccgactc tttttcgata atcagttagc aacgttactg gaagatcagg agaaagtagg
    15061 tacccacggg atcgtgatga tgattcgtct gccggatttc aatatgttga gcgatacttg
    15121 ggggcacagc caggttgaag aacagttctt cactctgact aatctgctgt cgacatttat
    15181 gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc agtgattttg ctgcgctgtt
    15241 accgcaccgg acgttaaaag aggcagagag catcgccggt cagttaatca aagccgtcga
    15301 taccttgccg aacaataaaa tgctcgatcg cgacgatatg atccacattg gtatctgcgc
    15361 ctggcgtagt ggtcaggata ccgagcaggt aatggaacat gcagagtctg ccacgcgtaa
    15421 tgcgggattg cagggcggca atagctgggc tatttacgat gactcgttgc ctgaaaaagg
    15481 acgcggtaat gttcgctggc gtacgcttat cgagcaaatg ctcagtcgcg gcggcccgcg
    15541 cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt catcatcgcg aactcatgtg
    15601 ccgcatcttc gatggtaatg aagaggttag ctcggcggag tatatgccga tggtcttgca
    15661 gtttggctta tcggaagagt atgaccgtct gcaaatcagc cgtcttattc cactattgcg
    15721 ttactggcca gaggaaaatc tggcgattca ggttaccgtt gagtcgctga ttcgcccgcg
    15781 ttttcagcgt tggctgcgcg atacgttaat gcaatgtgaa aaatcacaac gaaaacgcat
    15841 aattattgaa cttgcagagg ccgatgtagg tcaacatatc agtcgtttac aacctgttat
    15901 tcgtttagtg aatgctttag gggtacgggt agccgtcaac caggctggtt tgacgctggt
    15961 aagcaccagt tggatcaaag aacttaatgt tgagttactc aagctccatc cggggctggt
    16021 cagaaacatt gagaagcgaa cggagaacca gctgctggtt caaagcctgg tggaagcctg
    16081 ctccgggacc agcacccacg tttacgccac cggcgtgcgt tcgcgaagcg agtggcagac
    16141 cctgattcag cgcggtgtta caggcgggca aggggatttt ttcgcgtcct cacagccact
    16201 tgatactaac gtgaaaaaat attcacaaag atactcggtt taacctgccg tttaatccgt
    16261 tttcacgtag aataacgcgc gctgcgtctc atgggagtgt gcttgtctgc tcgccagatt
    16321 gttgcagcac atatgcagat gaatgacctt acgcggttgc aaacaggcga ggaatgctgc
    16381 tgatgcatta agcctttctg gactcaggca gagatttgta acaaaggaaa cgaactgcac
    16441 taattttcac cgtagcagat gatttttgcg ccttgtcgct gctgcgtgtg gttggtaaag
    16501 taagcggatt ttcttttccg ccccagcttt caggattatc ccttagtatg ttgaaaaaat
    16561 ttcgtggcat gttttccaat gacttgtcca ttgacctggg tactgcgaat accctcattt
    16621 atgtaaaagg acaaggcatc gtattgaatg agccttccgt ggtggccatt cgtcaggatc
    16681 gtgccggttc accgaaaagc gtagctgcag taggtcatga cgcgaagcag atgctgggcc
    16741 gtacgccggg caatattgct gccattcgcc caatgaaaga cggcgttatc gccgacttct
    16801 tcgtgactga aaaaatgctc cagcacttca tcaaacaagt gcacagcaac agctttatgc
    16861 gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc gacccaggtt gaacgccgcg
    16921 caattcgtga atccgcgcag ggcgctggtg cccgtgaagt cttcctgatt gaagaaccga
    16981 tggctgccgc aattggtgct ggcctgccgg tttctgaagc gaccggttct atggtggttg
    17041 atatcggtgg tggtaccact gaagttgctg ttatctcctt gaacggcgtg gtttactcct
    17101 cttccgtgcg cattggtggt gatcgtttcg acgaagctat catcaactat gtgcgtcgta
    17161 attacggttc tctgatcggt gaagccaccg cagaacgtat caagcacgaa atcggttcgg
    17221 cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg ccgtaacctg gcagaaggtg
    17281 ttccacgcgg ttttaccctg aactccaacg aaatcctcga agcactgcag gaaccgctga
    17341 ccggtattgt gagcgcggta atggttgcac tggaacagtg cccgccggaa ctggcttccg
    17401 acatctccga gcgcggcatg gtgctcaccg gtggtggcgc actgctgcgt aaccttgacc
    17461 gtttgttaat ggaagaaacc ggcattccag tagttgttgc tgaagacccg ctgacctgtg
    17521 tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat gcacggcggc gacctgttca
    17581 gcgaagagta atcggatgca ggcaggggaa atgtctgttt accctgcctg gtctgatacg
    17641 agaatacgca taacttatga agccaatttt tagccgtggc ccgtcgctac agattcgcct
    17701 tattctggcg gtgctggtgg cgctcggcat tattattgcc gacagccgcc tggggacgtt
    17761 cagtcaaatc cgtacttata tggataccgc cgtcagtcct ttctactttg tttccaatgc
    17821 tcctcgtgaa ttgctggatg gcgtatcgca gacgctggcc tcgcgtgacc aactagaact
    17881 tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac agtgaactgc tgatgcttgg
    17941 acaatacaaa caggagaacg cgcgtctgcg cgagctgctg ggttccccgc tgcgtcagga
    18001 tgagcagaaa atggtgactc aggttatctc cacggttaac gatccttata gcgatcaagt
    18061 tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc cagccggtca tcagcgacaa
    18121 aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc agtcgcgtgc tgctgatttg
    18181 tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac gatatccgcg taattgcagc
    18241 cggtaacggt tgtacggatg atttgcagct tgagcatctg ccagcgaata cggatattcg
    18301 tgttggtgat gtgctggtga cctccggtct gggcggtcgt ttcccggaag gctatccggt
    18361 cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct tatactgtga ttcaggcgcg
    18421 tccgactgca ggactgcaac gtttgcgtta tctgctgctg ctgtggggcg cggatcgtaa
    18481 cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt gctaatgaac gtctgatgca
    18541 gatgatgccg caggtattgc cttcgccaga cgcgatgggg ccaaagttac ctgaaccggc
    18601 aacggggatc gctcagccga ctccgcagca accgacgaca ggaaatgcag ctactgcgcc
    18661 tgctgcgccc acacagcctg ctgctaatcg ctctccacaa agggctacgc cgccgcaaag
    18721 tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag tggcgagcta tcgtagccag
    18781 ggacgttggg taatctggct ctctttcctc attgcgctgt tgctgcaaat catgccctgg
    18841 ccggataacc tgattgtttt ccggccaaac tgggtgttac tcatcttgtt gtattggatc
    18901 ctggccttgc ctcatcgcgt aaatgtgggc acaggttttg tgatgggtgc catactggat
    18961 ctgatcagcg gctcgacgct tggcgtacgc gtattggcga tgagcatcat tgcttacctg
    19021 gtggcgctga aataccagct tttccgcaac ctcgcattat ggcagcaggc gctggtcgtc
    19081 atgttgcttt cgctggtggt ggatattatt gttttctggg cagagttttt agtgattaac
    19141 gtctctttca gaccggaagt gttctggagt agtgtagtca atggggtgct ctggccgtgg
    19201 attttcttgc tgatgcgtaa agtccgtcag cagtttgcag tgcaataaag gtttctatga
    19261 cttctctgta tttagcttcc ggttctccgc gtcgtcagga gttacttgcg caacttggtg
    19321 tgacctttga acgtattgtt acgggcattg aggagcagcg tcagccgcag gagagcgcgc
    19381 agcagtatgt tgtgcgtctg gcgcgcgaga aagcacaggc gggtgtcgcg caaacggcgc
    19441 aggatctccc ggtgctgggt gcggatacta tcgttatcct gaatggagaa gtgctggaga
    19501 aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa attatcgggt cagacccatc
    19561 aggtgatgac ggcagtggcg ttggccgaca gccagcacat tctcgattgc ctggtggtca
    19621 ccgatgtgac tttcagaacg ttaacagacg aagacatcgc gggctatgtc gccagcggtg
    19681 aaccgttaga taaagcaggt gcatacggta ttcaggggct gggtggctgt tttgtcagga
    19741 agataaatgg cagctatcac gccgtagtcg gcttacctct ggttgaaacg tatgaattat
    19801 taagtaattt taacgcactg cgtgagaaaa gggataaaca tgacggctga attgttagta
    19861 aacgtaacgc cttcggaaac gcgagtggcg tatattgatg gcggtattct gcaggaaatt
    19921 catattgaac gtgaggcgcg acgcggaata gtaggcaata tctacaaggg tcgtgtaagt
    19981 cgtgtacttc cgggtatgca ggcggctttt gtagatattg ggctggataa agccgcgttt
    20041 cttcatgcat ccgatatcat gccgcacacc gaatgtgtgg cgggtgaaga acaaaagcaa
    20101 ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc aagatctgat ggtgcaggtg
    20161 gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca ccgatatcac gctgccttct
    20221 cgctatctgg tctttatgcc aggggcttct cacgttgggg tttcccaacg tattgaaagc
    20281 gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt attgcgacga gcagggcggg
    20341 tttatcatcc gtaccgcagc ggaaggggtt ggcgaggctg aactggcctc cgatgccgct
    20401 tatctgaaac gcgtctggac caaagttatg gagcgtaaaa agcgcccgca gacccgttat
    20461 cagctgtacg gcgaactggc gctggctcag cgtgttctgc gtgatttcgc cgacgccgaa
    20521 ctggaccgca ttcgcgttga ctcacgcctg acttacgaag cattacttga gttcacctcg
    20581 gagtacattc ccgagatgac cagcaagctg gagcattaca ccggacgcca gccgattttc
    20641 gatctctttg atgtcgaaaa cgaaatccag cgagcgctgg aacgcaaagt agaactgaaa
    20701 tccggtggtt atctcattat cgaccagacc gaagcgatga ccaccgtgga catcaatacc
    20761 ggagcgtttg tcggtcatcg caatctggac gacaccattt tcaataccaa tattgaagcg
    20821 acgcaggcta tcgctcgcca gttacggttg cgtaatctgg gcgggattat cattattgat
    20881 ttcatcgata tgaataatga agatcaccgc cgccgcgtgc tgcactcgct ggagcaggcg
    20941 ttgagcaaag accgggtgaa aaccagcgtt aatggttttt cggcgctggg gctggtggag
    21001 atgacgcgta aacgcacccg cgaaagcatt gagcacgtac tgtgtaacga atgcccaacc
    21061 tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat gctatgaaat catgcgcgag
    21121 attgttcgtg tccaccatgc ttacgactcc gaccgtttcc tggtctatgc ttctccggca
    21181 gtagccgaag ccttgaaagg cgaagagtca cactcgctgg cggaagtgga aattttcgtt
    21241 ggcaaacagg ttaaagtaca aattgaaccg ctctataacc aggagcagtt tgacgtcgta
    21301 atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt ttttgagtca catttttagc
    21361 agacaaggag tgacgggtga ggcgattgcc ggggatttta ctgcttactg gagccgcgct
    21421 cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt gctttaccgc atcttgacgc
    21481 ctggcgtccg gaaatcctca acaaaataga atccgcgact ggcatgccgg tagaagccag
    21541 tcagctctca gccagctggc agaattttgg cccgacgctt gaagcacacg acatccgtgc
    21601 agaactaaaa gatggcggcg aattttcggt taaacgcgtt actctggcgc tggatgtctg
    21661 gcagagcctg ttacatatgc gctggcagtt tcgcgacctc actttctggc agctgcgctt
    21721 tcgcaccaac actcctatca ccagcggtgg tagcgatgac agtctggaag ccagtcacat
    21781 cagcgatctg tttcttcgtc aatttgacca tttcgatctc cgcgacagtg aagtcagttt
    21841 cctgacgcca tccggtcagc gtgccgagct ggcgatccca caactcacct ggctgaacga
    21901 tccacgtcga caccgtgcgg aaggcctggt aagcctctcc agccttaccg gacagcacgg
    21961 cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg ttgttaagca atggtcgcgt
    22021 ctggctacag gcggatgaca tcgacctgaa gccgtggctc ggtaaatgga tgcaggacaa
    22081 tattgctctg gaaacggcac agttctccct tgaaggctgg atgacgatcg acaaaggcga
    22141 tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc agctggttgg gcgagaagca
    22201 aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt acgcgtgaaa atccgggctg
    22261 gcagttctct attcccgata cacggatcac gatggacggc aaaccctggc cgagcggagc
    22321 attgacgctg gcctggatac cggaacagga cgttggcggc aaagacaata aacgcagtga
    22381 cgaactccgg attcgcgcca gtaatctgga gctggcaggc ctggagggca tacgcccgct
    22441 ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc tccacacaac cgagcggcaa
    22501 gattaacact ctggcgctgg atatcccgct tcaggcggca gacaagaccc gttttcaggc
    22561 atcgtggagc gatctggcct ggaagcaatg gaaattatta ccgggtgcgg aacacttctc
    22621 cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc gcgtcgatga agcaggcaaa
    22681 gatgccttac gaaacggtat tccgtgcgcc actggaaatc gccgacggcc aggcaactat
    22741 aagctggctg aacaatgaca aaggtttcca gctggatggg cgtaatattg acgttaaagc
    22801 caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa cctgctaacg atgaaccctg
    22861 gctgggtatt ctggctggca tcagtaccga tgatggttca caagcctggc actatttccc
    22921 ggaaaacttg atgggtaaag acctggttga ttacttaagt ggcgcgattc agggcggtga
    22981 agcggataac gcgacgctgg tttatggtgg caatccgcaa ctcttcccct ataaacacaa
    23041 cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg aagtttgcct tccagccgga
    23101 ctggcctgca ttaactaacc ttgatattga actggacttt attaacgacg gtttatggat
    23161 gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt aatctcaccg cagtgatccc
    23221 tgactactcg aaagaaaaac tgctgattga cgctgacatt aaaggtccgg gtaaagccgt
    23281 tggcccttac tttgatgaga caccgctgaa agattctctg ggtgcgaccc tgcaagaact
    23341 ccagctcgac ggcgatgtga atgctcgctt acatcttgat atcccgctga acggcgagct
    23401 ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt ctgtttatca aaccactcga
    23461 cagcaccctg aaaaatttga gcggtaaatt cagctttatc aatggcgatc tgcaaagtga
    23521 accactgaca gcaagctggt ttaatcagcc gttgaacgtg gatttttcca ccaaagaagg
    23581 ggcaaaagcc taccaggttg cggtgaatct caacggtaac tggcaaccgg cgaaaaccgg
    23641 cgttctgcct gaagcggtga acgaagcatt gagtggcagc gtggcgtggg atggtaaagt
    23701 gggcattgat ctgccttatc atgctggcgc gacctataac gtagagctga acggcgatct
    23761 gaataatgtg agtagtcact taccttcacc gttagccaaa cctgcgggtg aaccactagc
    23821 ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa ttaaccggac aggctggtgc
    23881 ggataaccat ttcaatagcc gctggttgct tggtcaaaag ctgacgctcg atcgtgctat
    23941 ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa caaagtggcg ttgaactcaa
    24001 tatgccgccg atgaatggtg ccgagtggct ggccctgttc cagaaaggcg ctgcggagag
    24061 tgtcggtggt gcagcgagtt tcccacaata cataacgtta cgtacgccta tgttgtcact
    24121 gggaaatcag caatggaata acctgagtat tgtttcgcaa ccgacggcaa atggcaccct
    24181 ggttgaggcg caagggcgtg aaatcaacgc cacgctagcg atgcgtaata acgcgccgtg
    24241 gctggcgaat atcaaatatc tttattacaa cccgagcgtg gcgaaaactc gtggtgattc
    24301 aacgccgtca tcacctttcc cgacaacgga gcgcattaac ttccgtggct ggccggacgc
    24361 acaaatacga tgcacagagt gctggttctg ggggcaaaaa ttcggtcgca ttgacagtga
    24421 tatcaccatt tctggcgata cgttaacgct gaccaatgga ctgattgata ctggtttctc
    24481 gcggcttact gccgatggtg aatgggttaa taatccgggg aatgaacgta cctcgctgaa
    24541 aggaaaactg cgcgggcaga aaattgatgc cgccgcagaa ttttttggtg tcacgacgcc
    24601 catacgccag tcgtcattta atgtggatta cgatttacac tggcgcaaag caccctggca
    24661 gccagatgaa gcgacgttga atggcatcat tcatactcaa ctgggtaaag gcgaaattac
    24721 cgaaatcaat accggacatg ccgggcaatt gctgcgctta ttgagcgtag atgccctgat
    24781 gcgtaagctg cgttttgatt tcagagacac ttttggcgaa gggttctatt ttgactccat
    24841 tcgcagcacc gcgtggatta aagacggggt tatgcacacc gacgacacgc tggtggatgg
    24901 cctggaggcg gatatcgcca tgaaagggtc ggtaaatctg gtacgtcgcg acctgaatat
    24961 ggaagcggtt gtcgcaccag agatttctgc gacggtgggc gtggctgcgg cttttgcggt
    25021 taaccccatt gttggcgcgg cagtttttgc cgctagtaaa gtgctggggc cgctgtggag
    25081 caaagtctcc attttgcgct atcacatttc gggtccgctg gacgatccgc aaatcaacga
    25141 agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt gacgagggcg cgtaattgcc
    25201 ccaatctcat atgataatcg ttgccaaagg ccaacgagcc agaacataac cgtaggccgg
    25261 atagggcgtt catgccgcat ccggcagccg taaaaaatcc tctactgcag taactaacga
    25321 gtagcaaaaa cgatgagtct gaacctggta agtgaacaat tgctagcggc gaacagcctg
    25381 aaacatcagg acttgttcgc gatcctcggt caactggccg aacgtcgcct tgattatggc
    25441 gatctctatt ttcggtcgag ctatcacgaa tcctgggttt tagaagaccg cattattaaa
    25501 gatggttctt acaacatcga tcagggcgtt ggcgtgcgtg caatcagcgg tgaaaaaacc
    25561 ggatttgctt acgctgacca aatcagcctg ctggcgctgg aacagagtgc gcaagcggcg
    25621 cgcaccatcg tccgtgatag tggtgatggc aaagtacaga cgctgggcgc ggtagagcat
    25681 agcccgttgt atacctcggt agatccgctg caaagcatga gccgtgaaga gaagctggat
    25741 atcctgcgtc gcgtcgataa ggttgctcgc gaagcggaca agcgcgtaca ggaagtgact
    25801 gccagcctca gtggtgtcta tgaattaatt ctggttgcgg ccaccgacgg cacgctagcg
    25861 gcggatgtcc gtccgctggt gcgtctttcc gtgagcgttc tggtcgaaga agatggcaaa
    25921 cgcgaacgcg gtgccagtgg cggcggcggt cgttttggtt atgagttctt ccttgccgat
    25981 cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag cagtgcgtat ggcgctggtc
    26041 aatctttctg ccgttgctgc accagcgggc accatgccgg tagtacttgg cgcaggttgg
    26101 ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg aaggcgactt caaccgccgc
    26161 ggcacttcag tatttagtgg gcaggtcggg gagctggtgg cttcagaact gtgtaccgtg
    26221 gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg cgattgatga cgaaggtacg
    26281 ccaggccagt acaacgtgct gattgagaac ggcattctga aaggctacat gcaggataaa
    26341 ctcaacgcgc gtttgatggg gatgacgccg actggcaacg gtcgccgtga atcctacgcc
    26401 catctgccca tgccgcgtat gactaacacc tatatgctgc cgggtaaatc gaccccgcag
    26461 gaaattattg aatccgttga gtacggtatc tatgcaccga actttggtgg cggtcaggtg
    26521 gatatcacct ccggcaaatt cgttttctcc acttcagaag catatctgat tgaaaacggt
    26581 aaagtaacga agccggtgaa aggcgcaacg ttgattggtt ccggtatcga aaccatgcag
    26641 cagatttcga tggttggcaa cgacctgaaa ctggataacg gcgtgggtgt ctgcggtaaa
    26701 gaagggcaaa gtttgccggt tggcgtgggc cagccaacgc tgaaagttga taacctgact
    26761 gttggcggta ctgcgtaata atttcattat tttcagaagg ataattaatc tttctacgtg
    26821 cacgaacggt cccctcgctc ctctggggtt agggtgaggg gaacccgttg gcacaggttt
    26881 gtagaacgta acagtacaat atgaattact tctctttccc gcgcccgtgc atctcctgaa
    26941 acaatttacc gacctcaaca aaataatccg tcagcgagtt gatcacgacc tgtaccttca
    27001 gcggcagctt atctttttcg gtatataacg cataaaccgg gcgtggatct gactgataac
    27061 gcggcagcag gatctccagc tcaccgcgat tgatctcgtt gatcacccac atcagcggca
    27121 cgtaggcgat cccggcaccc gccgtcagcc agcgcaccag cgtcatcgga tcattagtca
    27181 caaatcttcc ttgtgggatc aggcgagtcg agatcccttc cggtgcgatc agttcaaatt
    27241 cattgtcggg ccgcacgctg tattcaagcc atgaatgact actcaaatcg gcgggttttt
    27301 ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca caccaccatc ggcatcgcgc
    27361 ccagacggcg ggaaaacagg ctggaattcc ggtaaccaga ttgacgctca aacctgggta
    27421 ttctttcagc atttttgctg tcagcccggc gagaacattt tgtgccatag ttgaagaaca
    27481 gccaatgcgc agcgtcccga tgggggtgtt attgaaggca tacagttgct catgaacatc
    27541 ctgcacttca tgaagcatcc gacggcagcc ctggtagtaa attctgccgg cttcggtcag
    27601 gccaatgctg cgtgtgctac ggtttaacag ctttacctgc aactcatctt ccagttttga
    27661 caccgtctga ctgatggacg aaacgctcat ctgtagctgt ctggcggcgg cggtaaaaga
    27721 gccaaattca actactttgg caaacaccga catgcgtttt agtcgttcca ttattcactc
    27781 tgacttaaaa gtgatttaga tcacataata tagataacag cataacagtt acgctaatat
    27841 attaaatatc aatctacagc aatgttgctc tcgcccggct ttccatgccc ttctctgcgg
    27901 cgacagatgc tgaaaataat aacgcctgct ctctctttac aaccaaggtc aacatgagtc
    27961 tgtttcccgt tatcgtggtg tttggactgt ccttcccacc gatatttttt gaattgcttt
    28021 tatcactggc gattttctgg ctggtgcgcc gggtacttgt gccaacaggt atctacgact
    28081 ttgtctggca tccggcgttg ttcaacaccg cgctctattg ctgcttgttt tatttgatat
    28141 cgcgactgtt cgtttgaggt tgaagtgaaa acactaataa gaaaattctc ccgtacggcc
    28201 atcacggtcg tattagtcat tctagccttc atcgcaattt ttaatgcctg ggtctattac
    28261 accgaatccc cctggacgcg tgacgcgcgc tttagcgctg acgtcgttgc gatcgcgccg
    28321 gacgtttctg gactcattac ccaggtgaat gttcatgata accagctggt gaaaaaagga
    28381 cagatactgt tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga agcgcaagcc
    28441 gatgttgctt attatcaggt actggcacag gagaaacgcc aggaggccgg acgtcgtaac
    28501 cgtctcggtg tgcaggcgat gtctcgcgaa gagatcgacc aggccaacaa cgtactacaa
    28561 acggttctgc atcagttagc gaaagcgcag gcgacccgcg atctggcaaa actggatctt
    28621 gaacgcacgg tgatccgcgc gccagcagat ggctgggtga ccaacctcaa cgtctatacc
    28681 ggtgagttta ttactcgagg atcaacggcg gttgcgctgg tgaaacagaa ctccttctat
    28741 gtactggcct atatggaaga aactaagctg gaaggggggc gtccggggta tcgtgcagag
    28801 atcacgccgc ttggcagtaa caaagtgctg aaagggactg ttgatagtgt tgccgcaggg
    28861 gtcaccaacg ccagcagcac gcgtgacgac aaagggatgg cgactataga ctctaatctt
    28921 gaatgggtgc gtcttgcgca acgtgttccg gttcgtattc gtctcgacaa ccagcaagag
    28981 aacatctggc ctgcgggcac cactgctaca gtggtggtca ctggcaaaca agatcgcgac
    29041 gaaagccagg attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt tggttaatca
    29101 cgatgggtat tttctccatt gctaaccaac atattcgctt tgcggtaaaa ctggcgaccg
    29161 ccattgtact ggcgctgttt gttggctttc acttccagct ggaaacgcca cgctgggcgg
    29221 tactgacagc ggcgattgtt gccgccggtc cggcctttgc tgcgggaggt gaaccgtatt
    29281 ctggcgctat tcgctatcgt ggctttttgc gcatcatcgg cacatttatt ggctgtattg
    29341 ccggactggt gatcatcatt gcgatgatcc gcgcaccatt attgatgatt ctggtgtgct
    29401 gtatctgggc cggtttttgt acctggatat cctcgctggt acgaatagaa aactcgtacg
    29461 cgtgggggct ggccggttat accgcgctga tcattgtgat caccattcag ccggaaccat
    29521 tgcttacgcc gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt attgtgtgtg
    29581 cgattatggc ggatttgctc ttttctccgc gatcgatcaa acaagaagta gatcgagagc
    29641 tggaaagttt gctggtcgcg caatatcaat taatgcaact ctgtatcaag catggcgatg
    29701 gtgaagttgt cgataaagcc tggggcgacc tggtgcgacg caccacggca ctacaaggca
    29761 tgcgcagcaa cctgaatatg gaatcttccc gctgggcgcg ggccaatcga cgtttaaaag
    29821 cgatcaatac gctatcgctg acgctgatta cccaatcctg cgaaacttat cttattcaga
    29881 atacgcgccc ggaattgatc actgatactt tccgcgaatt ttttgacacg ccggtagaaa
    29941 ccgcgcagga cgtccacaag cagctcaaac gcctgcggag agttatcgcc tggaccgggg
    30001 aacgggaaac gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt tatcagcttc
    30061 tcaagcgcgg cgttatcagt aacacaaaaa tcaacgccac cgaagaagag atcctgcaag
    30121 gcgaaccgga agtaaaagta gagtcagccg aacgtcatca tgcaatggtt aacttctggc
    30181 gaaccacact ttcctgcatt ctgggcacgc ttttctggct gtggacgggc tggacttccg
    30241 gcagtggtgc aatggtgatg attgcggtcg tgacgtcact ggcaatgcgt ttgccgaatc
    30301 cacgcatggt ggcgatcgac tttatctatg ggacgctggc cgcgctgccg ttagggctgc
    30361 tctacttttt ggtgattatc cctaataccc aacagagcat gttgctgctg tgtattagcc
    30421 tggcagtgct gggattcttc ctcggtatag aagtacagaa acggcgactg ggctcgatgg
    30481 gggcactggc cagcaccata aatattatcg tgctggataa cccgatgact ttccatttca
    30541 gtcagtttct cgacagcgca ttagggcaaa tcgtcggctg tgtgctcgcg ttcaccgtta
    30601 ttttgctggt gcgggataaa tcgcgcgaca ggactggacg tgtactgctt aatcagtttg
    30661 tttttgccgc tgtttccgcg atgactacca atgtggcacg tcgtaaagag aaccacctcc
    30721 cggcacttta tcagcagctg tttttgctga tgaataagtt cccaggggat ttgccgaaat
    30781 ttcgcctggc gctgacgatg attatcgcgc accagcgcct gcgtgatgcg ccgatcccgg
    30841 ttaacgagga tttatcggcg tttcaccgac aaatgcgccg cacagcagac catgtgatat
    30901 ctgcccgtag cgatgataaa cgtcgtcggt actttggcca gttgctggaa gaactggaaa
    30961 tctaccagga aaagctacgc atctggcaag cgccaccgca ggtgacggaa ccggttcatc
    31021 ggctggcggg gatgctccat aagtatcaac atgcgttgac cgatagttaa gtcaaaaccg
    31081 acgccaaaag cgtcggtttt ttcatggcta tacttagcga tgcacggcag aactcgccgc
    31141 gaaacgtgac ggtggcaaca gatgaatatt tatacctttg attttgatga gattgagagt
    31201 caggaggatt tttatcgtga ctttagccaa acctttggtc tggctaaaga taaggtacgc
    31261 gatctcgact cactatggga tgtgttaatg aacgatgtcc tgccgctacc acttgagatt
    31321 gaatttgttc atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat attgctgttt
    31381 gatgaggcag aggaagagct ggaagggcat ttgcgtttta atgttcgtca ttagcgcaaa
    31441 aaaaagcccc cgaaccgggg gcaatatcgt cggacaagac gatgagggtt tatttgtaca
    31501 gttcagccgt agcgtgccag gtgtcaccgc tacgagcttc agtaatctgg taggccgttg
    31561 cgcctttctc ttccgctttt ttgttcagca tttcacgcat atccattggc gaagacgcca
    31621 caccacttac ggatacggtc ccgattgctt cacgattttg tgcttgtgca gtatcaatgg
    31681 agtcggcagc gaatgcaccg aaagagagaa cagaaagtac gcttaatgca gctacagtgg
    31741 ttttgatttt catgattttt acctcgacat aatcttttag ctgggccttt gtttcgtgat
    31801 ccatatcaca aaatcaagca tacactaatt attgaactaa ttaataccac actaactgtt
    31861 tcagttgata ctaagtgtta aaaatgtgaa ttttaataac aaaaaagaat taaaaacacg
    31921 gttatttatt gttaattcta gtattttcaa ttagataatg aattttacat aatgtgcttt
    31981 ttttaccgta tgtattcatt gtgtgaatga tatgtcgcgg taaaacgcac tattcgttaa
    32041 attaatcgca ggggaaagca gggggttgag aggaataagc aacattttcc ccgccgccag
    32101 aagcgacggg gcagagatta aagctcctgg tcgaacagct ctaaaatcgc ttcgtacagg
    32161 tctttgacgg tgaaaccgtt agcaggggtg gtaaagatgg tgtcatcgcc agcgatggtg
    32221 cccagaatac cttctgcttt gcccagtgag tccagcaggc gagcaattaa ctgcgccgcg
    32281 ccagggctgg tatgaatcac gacaactgca tcgttgtagt cgatatccag caccagattc
    32341 ttcaatggac tggaggtggt tggtacaccc agttcagctg gcaggcagta aaccatttcc
    32401 attttggcat tgcgtgtacg tacagcacca aacttggtca acatccgcga gactttagac
    32461 tgattaatat tgtcaaagcc ttgctcctgc aacgcggcga cgatttcgcc ctgggagcta
    32521 aatttctctt ctttaagtaa tgctttaaat gctttaacta gttcttcttg cttagccgag
    32581 cttcgcataa gtcacccgat atggtggttg atacaacatt attgtgcata cagatgaatt
    32641 tttatgcaaa cagtcagccc tgaagaaggc tgaaataatg ttatgaaaga gcgggatttt
    32701 atcaaatttc gttattgaga aacatgcctg cgtcacggca tgcaaattct gtttaaaagt
    32761 aaattaattg ttatcaaatt gatgttgttt tggctgaacg gtagggtata ttgtcaccac
    32821 ctgttggaat gttgcgctaa tgcataagcg actgttaatt acgtaagtta ggttcctgat
    32881 tacggcaatt aaatgcataa acgctaaact tgcgtgacta cacattcttg agatgtggtc
    32941 attgtaaacg gcaattttgt ggattaaggt cgcggcagcg gagcaacata tcttagttta
    33001 tcaatataat aaggagttta ggatgaaagt cgcagtcctc ggcgctgctg gcggtattgg
    33061 ccaggcgctt gcactactgt taaaaaccca actgccttca ggttcagaac tctctctgta
    33121 tgatatcgct ccagtgactc ccggtgtggc tgtcgatctg agccatatcc ctactgctgt
    33181 gaaaatcaaa ggtttttctg gtgaagatgc gactccggcg ctggaaggcg cagatgtcgt
    33241 tcttatctct gcaggtgtag cgcgtaaacc gggtatggat cgttccgacc tgtttaacgt
    33301 taacgccggc atcgtgaaaa acctggtaca gcaagttgcg aaaacctgcc cgaaagcgtg
    33361 cattggtatt atcactaacc cggttaacac cacagttgcg attgctgctg aagtgctgaa
    33421 aaaagccggt gtttatgaca aaaacaaact gttcggcgtt accacgctgg acatcattcg
    33481 ttccaacact tttgttgcgg aactgaaagg caaacagcca ggcgaagttg aagtgccggt
    33541 tattggcggt cactctggtg ttaccattct gccgctgctg tcacaggttc ctggcgttag
    33601 ttttaccgag caggaagtgg ctgatctgac caaacgcatc cagaacgcgg gtactgaagt
    33661 ggttgaagcg aaagccggtg gcgggtctgc aaccctgtct atgggccagg cagctgcacg
    33721 ttttggtctg tctctggttc gtgcactgca gggcgaacaa ggcgttgtcg aatgtgccta
    33781 cgttgaaggc gatggtcagt acgcccgttt cttctctcaa ccgctgctgc tgggtaaaaa
    33841 cggcgtggaa gagcgtaaat ctatcggtac cctgagcgca tttgaacaga acgcgctgga
    33901 aggtatgctg gatacactca agaaagatat cgccttgggc gaagagttcg ttaataagta
    33961 attgattagc gaataataaa aaaccggagc acagac
//
LOCUS       DAJTYZ010000034        33247 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000034 DAJTYZ010000000
VERSION     DAJTYZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33247
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.034"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..664
                     /locus_tag="OO596_001543"
     CDS             <1..664
                     /locus_tag="OO596_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCR6717430.1"
                     /translation="SQAITNGFQRSMFSNEAGMGSTPNAAAAAASWPPHPAAQGIVQM
                     IGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQLIQKAMRVLMGSWGAEFVTLVVIL
                     FAFSSIVANYIYAENNLFFLRLNNPKAIWCLRICTFATVIGGTLLSLPLMWQLADIIM
                     ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPDIGRQLSPDAWDDVS
                     QE"
     gene            734..1510
                     /gene="yaaA"
                     /locus_tag="OO596_001544"
     CDS             734..1510
                     /gene="yaaA"
                     /locus_tag="OO596_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6717431.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1663..1959)
                     /locus_tag="OO596_001545"
     CDS             complement(1663..1959)
                     /locus_tag="OO596_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6717432.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(2173..3459)
                     /gene="thrC"
                     /locus_tag="OO596_001546"
     CDS             complement(2173..3459)
                     /gene="thrC"
                     /locus_tag="OO596_001546"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6717433.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(3460..4392)
                     /gene="thrB"
                     /locus_tag="OO596_001547"
     CDS             complement(3460..4392)
                     /gene="thrB"
                     /locus_tag="OO596_001547"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6717434.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(4394..6856)
                     /gene="thrA"
                     /locus_tag="OO596_001548"
     CDS             complement(4394..6856)
                     /gene="thrA"
                     /locus_tag="OO596_001548"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6717435.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6883..7002)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6938..7003)
                     /gene="thrL"
                     /locus_tag="OO596_001549"
     CDS             complement(6938..7003)
                     /gene="thrL"
                     /locus_tag="OO596_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6717436.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(7217..7903)
                     /locus_tag="OO596_001550"
     CDS             complement(7217..7903)
                     /locus_tag="OO596_001550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6717437.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(8303..8443)
                     /locus_tag="OO596_001551"
     CDS             complement(8303..8443)
                     /locus_tag="OO596_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717438.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8539..9255
                     /gene="arcA"
                     /locus_tag="OO596_001552"
     CDS             8539..9255
                     /gene="arcA"
                     /locus_tag="OO596_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6717439.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(9315..10667)
                     /gene="creD"
                     /locus_tag="OO596_001553"
     CDS             complement(9315..10667)
                     /gene="creD"
                     /locus_tag="OO596_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6717440.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10725..12149)
                     /gene="creC"
                     /locus_tag="OO596_001554"
     CDS             complement(10725..12149)
                     /gene="creC"
                     /locus_tag="OO596_001554"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6717441.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(12149..12838)
                     /gene="creB"
                     /locus_tag="OO596_001555"
     CDS             complement(12149..12838)
                     /gene="creB"
                     /locus_tag="OO596_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6717442.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12851..13324)
                     /gene="creA"
                     /locus_tag="OO596_001556"
     CDS             complement(12851..13324)
                     /gene="creA"
                     /locus_tag="OO596_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6717443.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13535..14404
                     /gene="robA"
                     /locus_tag="OO596_001557"
     CDS             13535..14404
                     /gene="robA"
                     /locus_tag="OO596_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6717444.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(14401..15048)
                     /gene="gpmB"
                     /locus_tag="OO596_001558"
     CDS             complement(14401..15048)
                     /gene="gpmB"
                     /locus_tag="OO596_001558"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6717445.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            15100..15621
                     /gene="yjjX"
                     /locus_tag="OO596_001559"
     CDS             15100..15621
                     /gene="yjjX"
                     /locus_tag="OO596_001559"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR6717446.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15706..16032)
                     /gene="trpR"
                     /locus_tag="OO596_001560"
     CDS             complement(15706..16032)
                     /gene="trpR"
                     /locus_tag="OO596_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6717447.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(16122..18059)
                     /gene="sltY"
                     /locus_tag="OO596_001561"
     CDS             complement(16122..18059)
                     /gene="sltY"
                     /locus_tag="OO596_001561"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6717448.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18270..19937
                     /gene="ettA"
                     /locus_tag="OO596_001562"
     CDS             18270..19937
                     /gene="ettA"
                     /locus_tag="OO596_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6717449.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(20244..21476)
                     /gene="nadR"
                     /locus_tag="OO596_001563"
     CDS             complement(20244..21476)
                     /gene="nadR"
                     /locus_tag="OO596_001563"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6717450.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21497..22879)
                     /gene="radA"
                     /locus_tag="OO596_001564"
     CDS             complement(21497..22879)
                     /gene="radA"
                     /locus_tag="OO596_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6717451.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22928..23896)
                     /gene="serB"
                     /locus_tag="OO596_001565"
     CDS             complement(22928..23896)
                     /gene="serB"
                     /locus_tag="OO596_001565"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6717452.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            24002..24646
                     /locus_tag="OO596_001566"
     CDS             24002..24646
                     /locus_tag="OO596_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6717453.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24674..25690
                     /gene="lplA"
                     /locus_tag="OO596_001567"
     CDS             24674..25690
                     /gene="lplA"
                     /locus_tag="OO596_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR6717454.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            25722..25871
                     /locus_tag="OO596_001568"
     CDS             25722..25871
                     /locus_tag="OO596_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717455.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(26146..26865)
                     /gene="deoD"
                     /locus_tag="OO596_001569"
     CDS             complement(26146..26865)
                     /gene="deoD"
                     /locus_tag="OO596_001569"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6717456.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(26945..28168)
                     /gene="deoB"
                     /locus_tag="OO596_001570"
     CDS             complement(26945..28168)
                     /gene="deoB"
                     /locus_tag="OO596_001570"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6717457.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(28220..29542)
                     /gene="deoA"
                     /locus_tag="OO596_001571"
     CDS             complement(28220..29542)
                     /gene="deoA"
                     /locus_tag="OO596_001571"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6717458.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(29669..30448)
                     /gene="deoC"
                     /locus_tag="OO596_001572"
     CDS             complement(29669..30448)
                     /gene="deoC"
                     /locus_tag="OO596_001572"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6717459.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            30706..32256
                     /locus_tag="OO596_001573"
     CDS             30706..32256
                     /locus_tag="OO596_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6717460.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            32228..33091
                     /locus_tag="OO596_001574"
     CDS             32228..33091
                     /locus_tag="OO596_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6717461.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         7906 a         9123 c         8376 g         7842 t
ORIGIN      
        1 aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg
       61 ttcgacgcca aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg
      121 gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat
      181 gctgatatta ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat
      241 ccagaaggcg atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt
      301 tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt
      361 ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac
      421 ggtcatcggc ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat
      481 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
      541 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
      601 gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga
      661 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
      721 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
      781 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca
      841 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
      901 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc
      961 ccgccaggct attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt
     1021 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
     1081 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
     1141 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
     1201 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
     1261 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
     1321 gaagaacggc aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag
     1381 tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
     1441 aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga
     1501 gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     1561 agcatgcaga ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg
     1621 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
     1681 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
     1741 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
     1801 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa
     1861 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
     1921 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
     1981 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
     2041 atcctaatct ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc
     2101 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
     2161 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
     2221 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
     2281 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
     2341 gaacaagcca tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc
     2401 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
     2461 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
     2521 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
     2581 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
     2641 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
     2701 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg
     2761 cagctgcgca actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
     2821 cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt
     2881 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
     2941 caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
     3001 cactttcaca ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
     3061 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
     3121 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
     3181 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
     3241 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
     3301 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
     3361 atgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
     3421 gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
     3481 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
     3541 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt
     3601 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc
     3661 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
     3721 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
     3781 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc
     3841 cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac
     3901 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
     3961 tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc
     4021 cagcaaacgg gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc
     4081 gaccaccgaa caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt
     4141 catcgccact ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac
     4201 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact
     4261 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt
     4321 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
     4381 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
     4441 caccagcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
     4501 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
     4561 cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa
     4621 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
     4681 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg
     4741 gcagcacagg ttcaatttca atatccgcca gttccagttc acgtcccgtt tcacgagcga
     4801 gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg
     4861 tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact
     4921 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac
     4981 ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
     5041 catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca
     5101 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt
     5161 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
     5221 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg
     5281 caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg
     5341 agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac
     5401 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
     5461 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
     5521 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
     5581 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
     5641 taccatcacc taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta
     5701 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt
     5761 cgctttgcgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
     5821 cggaaatacg ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt
     5881 tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga
     5941 ccggtaattc gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc
     6001 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
     6061 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
     6121 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
     6181 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
     6241 cgttgcgtcc aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca
     6301 gcaccatgtg atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat
     6361 cgacggtaga ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg
     6421 taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc
     6481 cacggcaaat cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat
     6541 gcaggacatg ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg
     6601 ggaatcccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
     6661 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
     6721 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
     6781 tttccagaat atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga
     6841 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact
     6901 gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg
     6961 taatggtgat ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg
     7021 taatttttat ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc
     7081 aataaaattt taatttactc atggcaggta accagttcag aagctgctat cagacactct
     7141 ttttttaatc cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata
     7201 cttactaagg cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca
     7261 acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt
     7321 ttatgtcatc tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt
     7381 gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta
     7441 attgatagca atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca
     7501 tcggcacacc agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt
     7561 cgcgaccaaa caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca
     7621 ccagttctac tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga
     7681 cagtgaaatc gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa
     7741 taacatcacc agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat
     7801 cgacaatccg cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat
     7861 tttctgctct ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga
     7921 ccaaataacc gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat
     7981 tcttaacgta agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc
     8041 tgactgtact aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat
     8101 aattttataa aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc
     8161 aactagttgg attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat
     8221 caaactgtta acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg
     8281 gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt
     8341 ttgacactgt cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt
     8401 tgatggaaag tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt
     8461 aatttgcagc atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg
     8521 gcaatttagg tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta
     8581 acacgcaaca cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca
     8641 gatggcgcgg aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat
     8701 atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat
     8761 gttgcgttga tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa
     8821 atcggtgcag atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca
     8881 cgcaacctgc tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt
     8941 gaaagctaca agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct
     9001 gatggcgagc agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa
     9061 aacccaggca aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg
     9121 aaaccacacg accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct
     9181 acgccggata cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt
     9241 gatctggaag attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt
     9301 tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg
     9361 gctttcattt tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg
     9421 ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc gacagagttg
     9481 agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg
     9541 cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg
     9601 gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa
     9661 aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg
     9721 agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta
     9781 gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg
     9841 aggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca
     9901 aaccagctgc tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc
     9961 aaaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta
    10021 cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc
    10081 ttcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct
    10141 tgctctaacc cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc
    10201 accacaccaa taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg
    10261 atatttggcg cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa
    10321 tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg
    10381 ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg
    10441 gttttatcct cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca
    10501 acaagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa
    10561 tcggcacgtt cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact
    10621 gcaccaaaca gggtggtcat tttccagaac agggaggatt tcaacatagt cttctccttt
    10681 gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg
    10741 tgaagtcgaa gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg
    10801 ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca
    10861 tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata
    10921 ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc
    10981 gttatacgac cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc
    11041 tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg
    11101 atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc
    11161 acatcaacag cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac
    11221 gtttccacca gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc
    11281 actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt
    11341 gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc
    11401 agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga
    11461 tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca
    11521 atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa
    11581 atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg
    11641 ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca
    11701 acatacatca ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac
    11761 tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta
    11821 tttgccgaat cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg
    11881 cgcactttgt taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac
    11941 gcctgagcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc
    12001 gccagcaacg ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta
    12061 acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag
    12121 cccagcagca accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc
    12181 gatgagtatt aatcggagaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt
    12241 taatgtgggt atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca
    12301 ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa
    12361 actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat
    12421 tcaattcaaa atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac
    12481 gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt
    12541 cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga
    12601 acagtacagg taacgccgga tggagcgcca gtaactggcg gcacaattca aagccgctga
    12661 tatcaggcaa accaacatcg agaatcatga cgtcgggaac ctgctggcga gctttatcca
    12721 gcaccggcaa gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga
    12781 ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa
    12841 atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg
    12901 aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat
    12961 aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct
    13021 cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac
    13081 aagaaatagc cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg
    13141 ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca
    13201 caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt
    13261 ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt
    13321 tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg taaataacat
    13381 attgattaag aacaaaatct caatactttt atttccgtca agccctaaaa catactctac
    13441 taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt
    13501 caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga
    13561 ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc
    13621 gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc
    13681 tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac
    13741 tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac
    13801 ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg
    13861 gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa
    13921 atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct
    13981 ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg
    14041 caacgcgccg accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga
    14101 taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg
    14161 ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta
    14221 tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc
    14281 aatgctcaac ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga
    14341 cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg
    14401 ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc
    14461 aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca
    14521 gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag
    14581 acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg
    14641 gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg
    14701 aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt
    14761 cagagaatcg atatgtcttt tctccagcac acccatgttt aattcacgca ggcgagaatc
    14821 aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc
    14881 taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat
    14941 cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc
    15001 ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg
    15061 tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt tatctgtgcg
    15121 accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa
    15181 ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc
    15241 agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca
    15301 gaggctgatt tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg
    15361 gtggtgattg aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct
    15421 gcggtgattc tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat
    15481 accggtattg atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa
    15541 ctcactcgcg ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat
    15601 gccgtgtatc aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc
    15661 acgatgcctg atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag
    15721 caacacctct tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc
    15781 acgcgtaatc gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc
    15841 gccacgcaac agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg
    15901 cgtcagcatc aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag
    15961 gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga
    16021 ttgttgggcc ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg
    16081 tactcgctaa agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat
    16141 tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa
    16201 gccagcacgt tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc
    16261 acagcgtcga tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg
    16321 ttataagctg ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg
    16381 taactggtgc caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa
    16441 ccaggaatag agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg
    16501 ccgctggccc ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc
    16561 gccatcgcat agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg
    16621 taagccagcg ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga
    16681 acgctaagat cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt
    16741 tttgacttgc tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag
    16801 tacatcaact cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc
    16861 tgcggcgctt tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc
    16921 gggtagaaac cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt
    16981 ccgcgttcca gcaataaatc cacctgccag taacgccatt catctttctc ttttgcttcc
    17041 atcggcagac gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt
    17101 cgcacacggc gttcaatcaa cgacgtggat tgcgagcgca taatgacgtc atcgcgccat
    17161 ttcgcctgct cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc
    17221 tcctgaatct gatcttcatt aagctgctgc acctgggcaa gcgacgggat catcagccgt
    17281 gcattttcag catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta
    17341 aaatcggtcg ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt
    17401 gaaatgattg ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt
    17461 accaggccag tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga
    17521 tcttgtttac ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc
    17581 ttgccggtta gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc
    17641 catttcgcat agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag
    17701 gctaacaagc cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc
    17761 gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt
    17821 tgattcatca gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc
    17881 ttcagtccag gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg
    17941 atttgcgcgt aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc
    18001 gtcagcagac agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca
    18061 agcgcatcct ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc
    18121 taaccagtca ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt
    18181 gatcgaccaa ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca
    18241 tcacgataaa catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg
    18301 tcggcaaagt tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc
    18361 ctggggcaaa aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca
    18421 ttatggcggg cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga
    18481 ttggttacct gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg
    18541 aagaagcggt ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt
    18601 acgccgatcc ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga
    18661 tcattcaggc tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc
    18721 tacgtctgcc ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg
    18781 tagcgttgtg ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca
    18841 accacctgga tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgagggca
    18901 ccgtggtggc gattacccac gaccgttact tcctcgataa cgttgcaggc tggatcctcg
    18961 aacttgaccg cggtgaaggt attccgtggg aaggcaacta ctcctcctgg ctggagcaga
    19021 aagatcagcg cctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga
    19081 aagagctgga gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc
    19141 tggcacgctt tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac
    19201 tgtttattcc acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta
    19261 aatcctacgg tgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcaa
    19321 tcgtcgggat catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctctg
    19381 gtcaggaaca gccggacagc ggcaccatca ctttaggtga aacggtgaaa ctggcgtcgg
    19441 ttgatcagtt ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg
    19501 ggctggatat tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct
    19561 ttaactttaa aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg
    19621 gtcgtctgca tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac
    19681 caaccaacga cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc
    19741 cgggctgtgc gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca
    19801 tcctggacta ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg
    19861 aagagtacaa gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca
    19921 agcgtattgc gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta
    19981 caaaccaaga aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc
    20041 gcatcaggca ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac
    20101 agccttagta tccgcaattt cgtagcgggt cacccgtgca ggttttttca aaccttgttt
    20161 aatctcgaca gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc
    20221 gcctttgagc cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc
    20281 acgcagcgca ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg
    20341 ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg
    20401 aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg
    20461 aaacggtatt catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg
    20521 cagaacgcct gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc
    20581 actgcaaaat caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc
    20641 aatgcgatct catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca
    20701 ctggtggtat tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg
    20761 ccaccaagga tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc
    20821 cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc
    20881 ggatcgacca gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct
    20941 tccgaggtgt agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg
    21001 ttgctccaca catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga
    21061 atgcgaatat ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc
    21121 gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa
    21181 cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt
    21241 ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga
    21301 aattcaagcc ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt
    21361 ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc
    21421 tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct
    21481 ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa
    21541 cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg
    21601 cccggcgaaa accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc
    21661 ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt
    21721 cacgcaggct ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct
    21781 tcacgccgcc gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac
    21841 ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc
    21901 gcggattcgc catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg
    21961 ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga
    22021 aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca
    22081 gctcattcac cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg
    22141 catcaccatc cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca
    22201 gcgagccatc tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga
    22261 agcgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta
    22321 catccgccat atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt
    22381 cggcaatcag gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg
    22441 gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat
    22501 acagcgtttt catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc
    22561 ccgcacctgg gttaccgcca atcagaatgg cgcttcccgg caccacgccg ccgcctagta
    22621 cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg
    22681 agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac
    22741 gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg
    22801 cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc
    22861 gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg
    22921 cgagcaatta cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag
    22981 cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc
    23041 ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct
    23101 gcgccagcgg gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg
    23161 cgtctacgat gtcgccaatc acattgccgg taaatttacc gtccatgatc tccagttcat
    23221 tggctaccac ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc
    23281 cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta
    23341 agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt
    23401 tcagcgtcgc cacacggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc
    23461 gttcggttac ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac
    23521 attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt
    23581 gcgggatttt ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc
    23641 gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa
    23701 caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc
    23761 cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat
    23821 caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc
    23881 aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg
    23941 aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa
    24001 gatggctcgc acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct
    24061 tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca
    24121 gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt
    24181 tgcaccgctg atgcgtaccg actcaccaga tgaaaaacgc attcaggcga tcctcgatca
    24241 gttaacggat gaaagccgca tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat
    24301 cgcccgttca ggcgaaagcg tcgaagtgcg cgaccggctg gcgctcgacg gtaaaaaagc
    24361 aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg
    24421 ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa
    24481 caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac
    24541 ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc
    24601 cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag
    24661 aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt
    24721 taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt
    24781 tctctggcgc aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg
    24841 taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg
    24901 cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta
    24961 cgataaaacc atctctacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc
    25021 cgaagcgtcc ggacgtaacg atctggtggt gaaaacagtc gaaggcgacc gcaaagtctc
    25081 aggctcggcc tatcgcgaaa ccaaagatcg tggcttccac cacggcactt tgctgctcaa
    25141 tgccgacctt agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa
    25201 aggcattacc tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac
    25261 ccatgagcag gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt
    25321 agaagcggaa atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt
    25381 tgcccgtcag agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct
    25441 ggatgaacgc tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat
    25501 cacccgcgcc caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg
    25561 gcgactgcaa ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt
    25621 ggttgacttc ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc
    25681 ggtaaggtaa ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc
    25741 aggagcggga gattatcaat ttaatgttaa gccagcgtat ttcctggcta gccgccgtgc
    25801 gtatcaggca taaattatca cttgctgaag taagtaaaat gttaggtatc tcgattaatt
    25861 ctctaaaata gatcgaaaag acggaacggc ttagctcaaa catcaaaagc aaaatgggag
    25921 aaatttatgg ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata
    25981 aatgaataga ttcgtgaata gatacttgaa tgtattttat tttttatcca tatgaaaaat
    26041 aatagtaaat ctataaatca gttgctattg atgaatagat actaaaaagc cggagcagtc
    26101 ccccactccg gcttaacacg acaatcgcct ttcagcgaaa cacaattact ctttatcgcc
    26161 cagcagaacg gattccagtg cgattttgat catgtcgttg aaggtggtct gacgctcagc
    26221 ggcagtggtc tgctcgtgag tgcggatgtg gtcagacaca gtgcagatgg tcagggcttt
    26281 cgcgccaaat tctgcagcga cgccgtagat accagccgct tccatttcca cgccgaggat
    26341 gccgtatttt tccatcacgt cgaacatttc gccgtccgga gagtagaaca ggtcagcgga
    26401 gaacaggtta ccaacgcgag catcaatgcc cagtgcttta gccgcgtcta ccgcgttacg
    26461 caccatgtca aagtcagcga tagcggcaaa gtcatgatct ttaaaacgga tgcggttaac
    26521 tttggaatcg gtgcaggcac ccataccgat aacgacgtcg cgcagtttta cgtgcggcag
    26581 aactgcgcca caggaaccca cgcggataat tttcttcacg ccgaaatcgg tgatcagttc
    26641 tttggtgtag atggagcagg acgggatacc cataccgtga cccattacgg aaattttgcg
    26701 gcctttgtaa gtaccggtga agcccagcat accgcgaacg ttgttcactt cacgggcatc
    26761 ttcaaggaaa gtttcagcaa tatacttcgc acgcagcggg tcgcctggca tcaaaactac
    26821 gtcagcgaaa tcgcccattt ctgcattaat gtgtggggta gccattgttt tatccttatc
    26881 aacatgtttt caccctctcc ctgagggaga gggccggggt gagggcatca agccgcaccc
    26941 attatcagaa catggctttg ccatattcca tatcagaagt accaaaatat tttgccagag
    27001 tctggccgat atccgcgaag gtttcacggt gacccagtga gcccggtttt actttcgggc
    27061 cgtaaaccag taccggaatg tgttcacgcg tgtggtcagt accggtccag gtcggatcac
    27121 aaccgtggtc agcagtgagg atcaggatgt cgtcatcgcg cagcagagac atcagctccg
    27181 gcagacggcg gtcgaacagc tccagaccca cagcataacc ggcgacgtcg cgacggtggc
    27241 cccaggaaga gtcgaagtca acgaagttgg tgaagacgat ggtgttatca cccgcttctt
    27301 tcatctcttt gatggtggcg tcaaacagcg cgtccaggcc agttgctttc actttcttgg
    27361 tgataccgca gttggcgtag atgtctgcaa ttttaccgac agaaaccacc tgaccgtgtt
    27421 tttcatcaac cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc aggtcgtgac
    27481 ggttaccggt acgctggaag ttaccggctt tgtcaccgat aaacggacga gcgataacac
    27541 gaccgatgtt gtagccgccg ttggtcagct cttcacgggc gatttcgcac agttcgtaga
    27601 gtttatccag gccaaaggtt tcttcgtggc aggcaatctg gaacacagag tcagcggagg
    27661 tatagaaaat cggcttgccg gttttcatgt gctcttcgcc cagctgatcc agaatgaccg
    27721 taccggaaga gtggcagtta ccgaggtaac ccggcagatt agcgcgttca accagtttat
    27781 ccagcagctc ttgcgggaag ctgttttcgt gatcggagaa atatccccac tcaaatagaa
    27841 ccgggacacc ggctatttcc cagtgaccag acggggtatc tttaccggat gacatttcgt
    27901 gcgcccatgc gtacgcgccg ataacttcag cgttgccgtc cattcccgcc ggaatgaaac
    27961 cggtagaacc ttcgtgtgct ttcgccagcc ccagacgggt cagatttggc agattgagcg
    28021 gacctttacg accgttatca gcttcgcctt tggcacaagc ttctgcgata tgacccaggg
    28081 tgtcagcccc gacgtcacca aagcgttctg catcttctgt agcgccgatg ccgaatgagt
    28141 ccagcaccat aataaatgca cgtttcatat gttctccgta ctcagtgctt caaatttaaa
    28201 tgatcagatc agtataccgt tattcgctga tacggcgata gacagttggt gtgctttccg
    28261 gtgctttatc ggcaagttta attgccgctt tcaccgcttt cgccgcttcc tgccagctgt
    28321 tttcgtcttt cgcgtggata accgccagcg gacgctgacc gtctacctgg tcgcccaaac
    28381 gcgccatatc agtaaagccg acgctgtaat caatggtgtc agatgcctga cgacgaccgc
    28441 cgcccattgc aaccactgcc atccccagcg cgcgggtatc catttcactg acaaaacctt
    28501 cggtatcagc atagactgct ttcgtcagca tcgctgtcgg cagatacttc gcgtagttct
    28561 caacgaagtc ggttgggcct ttttgtgccg ctaccatacg accaaagact tctgccgctt
    28621 taccgttgtc cagcactgct tgcaatttcg cgcgcgcttc ggcgtcatct ttcgccagtt
    28681 tgccggagat aagcatctcc acgcacagcg ccatcgtgac atcaaacaga cgcgggttac
    28741 gatactcacc cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta cctgcactgg
    28801 aggccagtac ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca gcgccgttag
    28861 ccacgccaac aatcgcttcg gcaagggctt cagagagttc gtaggtcggc ataaacgcgc
    28921 cgctacccac tttcacgtcc atcaccagcg cgtccagacc ttccgcaagt ttcttcgcca
    28981 gaatagaggc ggtgatcagc gggatggagt ccacggttgc ggtaatatca cgggtcgcgt
    29041 agaaacgttt atccgccgga gccagtgagc tggtctgacc gataatcgcc acgccgacgt
    29101 ctttaataat ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca gggatggatt
    29161 ccagtttgtc gagcgtaccg ccagtatgac cgaggccgcg accagagatc atcggaatat
    29221 agccgccgca ggctgcgacc atcggcccca acatcagcga agtcacatcg ccgacgccac
    29281 cagtggaatg tttatcaaca atcgggccat tcagatgcag gcttttccag tcgagaacgg
    29341 ttcctgaatc tcgcatcgcc atggtcagcg agacacgctc aggcattgtc atatcgtgga
    29401 agaaaatggt catcgcgagg gcggcaatct gcccttcgga gatagtgttg tcgcgaatgc
    29461 cgttgataaa gaaacgaatt tcttcatcgc tcagtgcatg accatcacgt tttttacgaa
    29521 taatttcttg tgcgagaaac aaggtaacct cccgaggaaa agagtatgag gcggcttttg
    29581 ccggatgcgt ctacgcctta tccggcctac gggtagatac gtctcgtagg cctgataagc
    29641 gcagcgcatc aggcgtaaag catcttactt agtagctgct ggcgctctta ccgtcaccgt
    29701 ggcccagcgc tttcaacagg cttgccagca ggctggaagc accaaagcgg tagtgacgcg
    29761 catctgccca gtcagcaccg aacagttcat ctgcgatggc gagatatttc tgcgcatctt
    29821 ccgcagtacg cacgccgccc gctggtttga aaccaacggt tttttctacg cccatatcac
    29881 ggatcacttc catcatgatg cgcgcgcttt ccggcgtcgc gttcacagcc actttaccgg
    29941 tagaggtttt gatgaagtcc gcacccgctt tgatggagat ttcagacgct ttacggatca
    30001 gcgcttcgtc tttcagttcg ccggtttcga tgatcacttt cagcagtaca ttcgctgccg
    30061 cgcaagcctc tttacaggct ttcaccaggt caaaaccaac ctgctcgtta cccgccatca
    30121 gcgcgcggta cgggaacaca acgtcaactt catcagcacc gtaggcgatt gccgcacggg
    30181 tttctgccag cgcgatttcg atgtcatcgt taccgtgtgg gaagttggtt accgtagcga
    30241 ttcggatttc cggggtgccc tgctctttca gcgttttgcg agcaatcggg ataaagcgag
    30301 gatagataca gatagcggcg gtattgccga ccggggtttt ggcctgatga cacagagcaa
    30361 ttactttctc gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac
    30421 gcaggctgct tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc
    30481 accttgcgag tttgttagta ttctaacatc tactccgcaa cacacttcga tatacatcac
    30541 aattaaggaa atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc
    30601 actttcaaat gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa
    30661 acttcttaca atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat
    30721 gaaaatgcac tgcaacaacg ttgccagcaa attgtcacca gcccagtgct tagcccggag
    30781 cagaagcgcc attttctggc actggaagca gaaaacaatc tgccttatcc gcagcttcct
    30841 gccgaagccc gccgcgcgct ggatgagggt gtaatctgcg atatgtttga aggtcatgcg
    30901 ccgtacaaac cgcgctatgt cttacccgat tacgcccgtt ttctggcgaa cggttccgaa
    30961 tggctggagc tggaaggcgc gaaagatctt gatgacgcac tctctctgct gaccattctt
    31021 taccaccacg taccgtcggt cacatcgatg ccggtctacc tggggcaact ggatgcgttg
    31081 ttgcaaccat atgttagaat tctaacacaa gacgagatcg atgttcgaat aaaacgtttc
    31141 tggcgttacc tcgacagaac cctgccagac gcctttatgc acgccaatat cggcccgtct
    31201 gattcgccca ttacccgcgc gatcttacgt gcagacgcag agttgaagca ggtttcaccg
    31261 aacctgacct ttatctacga tcctgaaatc acccctgatg acctgctgct ggaagtggcg
    31321 aagaacatct gtgaatgtag caaaccgcac atcgccaacg gtccggtgca tgataaaatt
    31381 ttcacaaaag ggggctacgg gattgtgagc tgttacaact cactgccgct ggcgggtggt
    31441 ggcagcacgc tggtacgcct taacctgaaa gccattgccg agcgcagcga atcgctggat
    31501 gacttcttta cgcgcactct accgcactac tgccagcagc agatcgccat catcgatgcg
    31561 cggtgtgaat tcctctatca acaatcacac ttctttgaga atagcttcct ggtgaaagaa
    31621 gggctgatta accctgaacg ttttgtgcca atgtttggca tgtatgggct ggcggaagcg
    31681 gttaacttgc tgtgtaagaa agaagggatt gccgcgcgct acggtaaaga agccgccgca
    31741 aatgaagtgg gttatcgcat cagcgcgcaa ctggcggagt ttgtcgccaa tacccccgtg
    31801 aaatatggct ggcaaaaacg cgccatgtta cacgcacagt cggggagcag ttccgatatc
    31861 ggcaccacgc cgggcgcgcg tttgccgtat ggtgatgagc cagatccgat cacccatctg
    31921 caaactgtcg ccccgcatca tgcttattat tattccggca tcagcgacat tctgacgctc
    31981 gacgaaacca tcaaacgtaa tccgcaggcg ctggtacagc tttgcctcgg tgcctttaaa
    32041 gccggaatgc gtgaatttac tgccaatgtc agcggtaacg atctggttcg cgttaccggt
    32101 tatatggtgc gtttgtcgga tttagaaaaa tatcgcgccg aaggttcacg caccaacacc
    32161 acctggctgg gcgaagaagc cgcacgcaac actcgtattc tggaacgcca gccgcgcgtg
    32221 ataagccatg aacagcagat gcgctttagt cagtaagatt atccccttct cctgcgttga
    32281 cgggccaggc agtcgtctgg ctctgttttt gcagggctgc aatctgcgct gcaaaaactg
    32341 tcacaatccg tggacgatgg gacgttgcaa tgactgtggg gagtgcgtgc cacagtgtcc
    32401 gcatcaggcg ttgcagattg ttgacggcaa agtggtgtgg aacgctgtgg tttgcgagca
    32461 gtgtgatacc tgcctgaaga ggtgtccgca acatgccacg ccaatggcgc aatccatgag
    32521 cgtggacgaa gtgcttagcc atgtccgcaa agcggtgctg tttattgaag ggataacggt
    32581 aagcggcggc gaagccacga cccaactgcc gtttgtagtg gcgctgttta ctgctatcaa
    32641 aaacgatccg caactgcgcc acctcacctg cctggtggac agtaacggca tgttgagcga
    32701 aaccggctgg gaaaaattac tcccggtgtg tgacggcgca atgctcgatc tcaaagcgtg
    32761 ggggagcgaa tgtcatcaac acttaaccgg acgcgataat cagcagatta agcgcagcat
    32821 ctgtttgctt gcagagcgcg gcaagctggc ggaactgcgt ttgctggtaa ttccagacca
    32881 ggtggattat ttgcatcaca tcgatgagct ggcgacgttt atcaagagac ttggcgatgt
    32941 tccggttcgc ctgaatgcgt ttcatgccca cggcgtgtat ggcgaggcgc aaagctgggc
    33001 gagcgccacg ccggaagacg ttgagccgtt ggctgatgcg ttaaaggtgc gcggggtgag
    33061 ccggttgata tttccggcgc tctatttgtg acatgccgga tacggcctac ggttcggcac
    33121 agacttgtag gcatgataag acgcgacaag cgtcgcatca ggcattatgc gccgactgcc
    33181 ggatgcggcg tgaacgcctt atccggccta cggttcggca cagacttgta ggcatgataa
    33241 gacgcgt
//
LOCUS       DAJTYZ010000035        32533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000035 DAJTYZ010000000
VERSION     DAJTYZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.035"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..94
                     /locus_tag="OO596_001575"
     CDS             <1..94
                     /locus_tag="OO596_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6717462.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="OO596_001576"
     CDS             complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="OO596_001576"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6717463.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="OO596_001577"
     CDS             complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="OO596_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6717464.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2848..4476
                     /gene="glpA"
                     /locus_tag="OO596_001578"
     CDS             2848..4476
                     /gene="glpA"
                     /locus_tag="OO596_001578"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6717465.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4466..5725
                     /gene="glpB"
                     /locus_tag="OO596_001579"
     CDS             4466..5725
                     /gene="glpB"
                     /locus_tag="OO596_001579"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6717466.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGPESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5722..6912
                     /gene="glpC"
                     /locus_tag="OO596_001580"
     CDS             5722..6912
                     /gene="glpC"
                     /locus_tag="OO596_001580"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6717467.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7105..8007
                     /locus_tag="OO596_001581"
     CDS             7105..8007
                     /locus_tag="OO596_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6717468.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="OO596_001582"
     CDS             complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="OO596_001582"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR6717469.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8869..10158)
                     /locus_tag="OO596_001583"
     CDS             complement(8869..10158)
                     /locus_tag="OO596_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6717470.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="OO596_001584"
                     /pseudo
     CDS             complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="OO596_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11434..12216)
                     /locus_tag="OO596_001585"
     CDS             complement(11434..12216)
                     /locus_tag="OO596_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR6717471.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12436..13638)
                     /locus_tag="OO596_001586"
     CDS             complement(12436..13638)
                     /locus_tag="OO596_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6717472.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13738..14280)
                     /locus_tag="OO596_001587"
     CDS             complement(13738..14280)
                     /locus_tag="OO596_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717473.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14559..14984
                     /gene="nudI"
                     /locus_tag="OO596_001588"
     CDS             14559..14984
                     /gene="nudI"
                     /locus_tag="OO596_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6717474.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15023..15625)
                     /locus_tag="OO596_001589"
     CDS             complement(15023..15625)
                     /locus_tag="OO596_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6717475.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15933..17072
                     /gene="arnB"
                     /locus_tag="OO596_001590"
     CDS             15933..17072
                     /gene="arnB"
                     /locus_tag="OO596_001590"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6717476.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17076..18044
                     /gene="arnC"
                     /locus_tag="OO596_001591"
     CDS             17076..18044
                     /gene="arnC"
                     /locus_tag="OO596_001591"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6717477.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18044..20026
                     /gene="arnA"
                     /locus_tag="OO596_001592"
     CDS             18044..20026
                     /gene="arnA"
                     /locus_tag="OO596_001592"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6717478.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20023..20913
                     /locus_tag="OO596_001593"
     CDS             20023..20913
                     /locus_tag="OO596_001593"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6717479.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20913..22565
                     /gene="arnT"
                     /locus_tag="OO596_001594"
     CDS             20913..22565
                     /gene="arnT"
                     /locus_tag="OO596_001594"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6717480.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22562..22897
                     /gene="arnE"
                     /locus_tag="OO596_001595"
     CDS             22562..22897
                     /gene="arnE"
                     /locus_tag="OO596_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6717481.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22897..23283
                     /gene="arnF"
                     /locus_tag="OO596_001596"
     CDS             22897..23283
                     /gene="arnF"
                     /locus_tag="OO596_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6717482.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="OO596_001597"
     CDS             complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="OO596_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6717483.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23587..25008)
                     /gene="menE"
                     /locus_tag="OO596_001598"
     CDS             complement(23587..25008)
                     /gene="menE"
                     /locus_tag="OO596_001598"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6717484.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25005..25967)
                     /gene="menC"
                     /locus_tag="OO596_001599"
     CDS             complement(25005..25967)
                     /gene="menC"
                     /locus_tag="OO596_001599"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6717485.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25967..26824)
                     /gene="menB"
                     /locus_tag="OO596_001600"
     CDS             complement(25967..26824)
                     /gene="menB"
                     /locus_tag="OO596_001600"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6717486.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26839..27597)
                     /gene="menH"
                     /locus_tag="OO596_001601"
     CDS             complement(26839..27597)
                     /gene="menH"
                     /locus_tag="OO596_001601"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6717487.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27594..29264)
                     /gene="menD"
                     /locus_tag="OO596_001602"
     CDS             complement(27594..29264)
                     /gene="menD"
                     /locus_tag="OO596_001602"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6717488.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29353..30648)
                     /gene="menF"
                     /locus_tag="OO596_001603"
     CDS             complement(29353..30648)
                     /gene="menF"
                     /locus_tag="OO596_001603"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6717489.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="OO596_001604"
     CDS             complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="OO596_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6717490.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31087..31548)
                     /locus_tag="OO596_001605"
                     /pseudo
     CDS             complement(31087..31548)
                     /locus_tag="OO596_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31613..32530
                     /gene="rbn"
                     /locus_tag="OO596_001606"
     CDS             31613..32530
                     /gene="rbn"
                     /locus_tag="OO596_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6717491.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7896 a         8806 c         8139 g         7692 t
ORIGIN      
        1 ttcgttgttg gctaaagaaa agccggtaga aggcagtcca ctgtttttac tacaaatgtg
       61 catggaacaa ttagtggcga ttggaaaaat ttgatatgag acaggatggc gcatgaacgc
      121 catcctgcag tagcattact ctttattaag gaattttact gccttatcag ggaaatcagt
      181 aaacagccca tttacacccg ctttgttata cagagcatca tataactgat tcacatcagt
      241 agtgtattca ggcagtttat ccgaccgcac ggtataagga tgcactacca gtttattctg
      301 ctgagcatct tgcaccatgc cagtgagttt gatattaccc ggctgcgatg tttcctcaat
      361 caacatatgg taatccggcc cgataccgtc ggcatattcc gcgacttgct tcatggcacc
      421 tggcttaaac atccagtcat agctgtaatt aacccagctt ccgtccggct gtttctgctg
      481 cgtttcattc cagtcggtat aggcaatcag ctgtaccaga ttgagatcca tgcccatttt
      541 aggttccagc tcattcttaa tacgcttcag ctcatcagca tcaaaacatt gcaaataaac
      601 tttatcgtcc ttaccggtgt aaccatattt cttcagcact tccagcgttt ttgccgcaat
      661 atccttccct tcctgatgat ggaaccacgg cgctttgatt tctggataga taccgatatt
      721 tttcccggta gagtgattta acccctgaac aaattcaatc tcttcttcaa aggtgtgccc
      781 ccggaagtcg gacttaccca ttgggaaacg ccccggataa gtctgtactt ttttaccgtt
      841 ttcaatatcg aaaccttcgg taaatttcag cgacttaatt tcatccagcg tgaaatctat
      901 cgcgtagtaa cgaccgtctt tgcgcgcccg atccgggaaa cgatcggcaa catcagtaac
      961 acgatcgaga taatggtcat gcagaacaac cagatggtcg tctttggtca tcaccaaatc
     1021 ctgttccaga taatccgctc cctgcgcata cgccatcgct tttgctggca gcgtatgctc
     1081 cggcaaatat ccactggcac cgcgatgggc gatgactatt ttttcgttgc tgtccgccgc
     1141 cattgcactg cttcccatga ctatagtgct catcatgatc gccatgctaa ggtttttcag
     1201 cttcaatttc atgccattag cctccgttgc gtttttgcag taattgttca tggcgacgtt
     1261 tttcgccaat catcacaaca atcaacaaga taaccgccag aatgctgccg ccaatcatta
     1321 ccataaagcc gccatcccag ccgaagaagt ccacggtgta gccaacaatc gcgctcgccg
     1381 ccaccgaacc gcccaggtaa ccaaacagcc cggtaaagcc cgctgccgta cctgccgctt
     1441 ttttcggtgc cagttccagc gcatgcagac cgatcagcat cacaggaccg tagatcagga
     1501 agccgataac aatcatacaa atcatatcga cggttgggtt acctgccggg ttcatccagt
     1561 aaacgatagt cgcgatggtc accagtgtca taaagaaaac gccggttgcc ccacggttgc
     1621 cacggaagac tttatccgac atccagccgc acagcagagt gcccggaata cctgcatatt
     1681 cataaaggaa gtaggcccag gaggatttat ccagcgcgaa atgcttaacc tctttcagat
     1741 aagtcggtga ccagtcgagg atgccgtaac gcagcagata aacgaacacg ttggcgatgg
     1801 cgatatacca cagcagtttg ttcggcagta cgtactgcat gaagatttgc ttcgccgtca
     1861 gctcctgttc cgctttttcg ttatagtcgt ccggataatc atttttgtac tcttcgatcg
     1921 gcggcaagcc acaggattgc ggggtatcgc gcatcatcgc aaaggcgaat aatgccacca
     1981 gaatggcgca gaaagcaggc atatagagcg ccgcatgcca gtcattgaac caggccatcc
     2041 ccagcaggaa cagcagcggc ggaataccac caccgacgtt gtgcgcacag ttccacactg
     2101 acacaatgcc gccacgttct ttctgcgacc accagtgcac catagtacga ccacacggcg
     2161 gccaccccat cccctggaac caaccgcaga ggaacaacag tacaaacatc accgcaatgc
     2221 tcgacgtcgc ccatggcaca aagcccataa acaacatcac tgccgccgcc agaatcaaac
     2281 ctgcgggcag gaaaacgcgc ggattcgagc gatccgatac cgaacccatg atgaatttcg
     2341 aaaatccata agcaatcgag atccccgaaa gggcaaaacc taaatcaccg cgtgagaatc
     2401 cctgctcaac cagataaggc atagcaagcg caaagttctt acgaaccaaa tagtaagccg
     2461 catagccaaa gaatatcccc aggaaaattt gccagcgcaa tcgacgataa gtcggatcga
     2521 tctccgcggc aggtaagcgc gctttgtgtg gcgctggttt aaaaatactc aacattgata
     2581 gcctccgtgg cccgtggtct tatttatgat taacagcctg attcagtgag agaacctgcc
     2641 gtttcttgag ttgccgcgat gttaagaaaa cattcataaa ttaaatgtga attgccgcac
     2701 acattattaa ataagattta caaaatgttc aaaatgacgc atgaaatcac gcttcacttt
     2761 cgaatcatga gcgaatatgc gcgaaatcaa acaattcatg tttttactat ggctaaatgg
     2821 taaaaaacga acttcagagg gataacaatg aaaactcgcg actcgcaatc aagtgacgtg
     2881 attatcattg gcggcggcgc aacgggagcc gggattgccc gcgactgtgc cctgcgcggg
     2941 ctgcgcgtga ttttggttga gcgccacgac atcgcaaccg gtgccaccgg gcgtaaccac
     3001 ggcctgctac acagcggtgc gcgctatgcg gtaaccgatg cggaatcggc ccgcgaatgc
     3061 attagtgaaa accagatcct gaaacgcatt gcacgtcact gcgttgaacc aaccaacggc
     3121 ctgtttatta ccctgccgga agatgacctc tccttccagg ccacttttat tcgcgcctgc
     3181 gaagaagcag ggatcagcgc agaagctata gacccgcagc aagcgcgcat tatcgaacct
     3241 gccgttaacc cggcattgat tggcgcggtg aaagttccgg atggcaccgt tgatccattt
     3301 cgtctgaccg cagcaaacat gctggatgcc aaagaacacg gtgccgttat ccttaccgct
     3361 catgaagtca cgggactgat tcgtgaaggc gcgacggtgt gcggtgttcg tgtacgtaac
     3421 catctcaccg gcgaaactca ggcccttcat gcgcctgtcg tggttaatgc cgccgggatc
     3481 tgggggcaac acattgccga atatgccgat ctgcgcattc gcatgttccc ggcgaaagga
     3541 tcgctgctga tcatggatca ccgcattaac cagcatgtga tcaaccgctg ccgtaaacct
     3601 tccgacgccg atattctggt gcctggcgat accatttcgc tgattggcac cacctcttta
     3661 cgtattgatt acaacgagat tgacgataat cgagtgacgg cagaagaggt tgatattctg
     3721 ctgcgtgaag gggaaaaact ggcccccgtg atggcgaaaa cgcgcatttt gcgggcctat
     3781 tctggcgtgc gcccgctggt tgccagcgat gacgacccga gcggacgtaa cgtcagccgt
     3841 ggcatcgtgc tgctcgacca tgctgaacgc gatggtctgg acggatttat caccatcacc
     3901 ggtggcaaac tgatgaccta tcggctgatg gctgaatggg ctaccgacgc cgtgtgccgc
     3961 aaactgggca acacgcgccc ctgtacgacc gccgatctgg cacttcctgg ttcacaggaa
     4021 cccgctgaag ttaccttgcg taaagtcatc tccctgcctg ccccgctgcg cggttctgcg
     4081 gtttatcgtc atggcgatcg cacgcctgcc tggctgagcg aaggtcgtct gcaccgtagc
     4141 ctggtatgtg agtgcgaagc ggtaacagcg ggtgaagtgc agtacgcggt agaaaattta
     4201 aacgttaata gcctgctgga tttacgccgt cgtacccgtg tggggatggg tacctgccag
     4261 ggcgaactct gcgcctgccg cgctgccgga ctgctgcaac gttttaacgt cacgacgtcc
     4321 gcgcaatcta tcgagcaact ttccaccttc cttaacgaac gctggaaagg cgtgcaaccc
     4381 atcgcctggg gagatgcact gcgcgaaagc gaatttaccc gctgggttta tcagggactg
     4441 tgtggtctgg agaaggagca gaaagatgcg ctttgatact gtcattatgg gcggcggcct
     4501 cgccggatta ctctgtggcc tgcaactgca aaaacacggc ctgcgctgtg ccattgtcac
     4561 gcgtggtcaa agcgcgctgc atttctcatc cggttcgctg gatttgctga gccatctgcc
     4621 tgatggtcaa ccggtgacag acattcacag tggaccggaa tctttgcgtc agcaggcacc
     4681 agcccatcct tacagtcttc tcgaaccaca acgcgtgctc gatctcgctt gccaggcgca
     4741 ggcattgatc gctgaaagcg gtgcgcaatt gcagggcagc gtggaactgg cccatcagcg
     4801 cgttaccccg ctcggcactt tgcgtgcaac ctggttaagt tcgccagaag ttcccgtctg
     4861 gccgctaccc gcgaagaaga tatgtgtagt ggggataagc gggctgatgg atttccaggc
     4921 gcatcttgcg gcggcttcgt tgcgcgaact ggatctttcg gttgaaaccg cagaaataga
     4981 gctgccggaa ctggatgtcc tgcgcaataa cgccaccgaa tttcgcgcgg tgaatatcgc
     5041 ccgtttcctt gataatgaag aaaactggcc gctgttactt gatgcgctta ttcctgtcgc
     5101 caatacctgc gaaatgatcc tgatgcccgc ctgcttcggc ctggccgatg acaaactgtg
     5161 gcgttggttg aatgaaaaac taccttgttc actgatgctt ttgccaacgc tgccgccttc
     5221 cgtgctgggc attcgtctgc aaaaccagtt acagcgccag tttgtgcgcc agggtggcgt
     5281 gtggatgccg ggcgatgaag tgaaaaaagt gacctgtaaa aatggcgtag tgaatgaaat
     5341 ctggacccgc aatcacgccg atattccgct acgtccacgt ttcgcggttc tcgccagcgg
     5401 cagtttcttt agtggcggac tggtagcgga acgtaacggc attcgcgagc cgattctcgg
     5461 ccttgatgtg ctacaaaccg ccacgcgggg tgaatggtat aagggcgatt tttttgcgcc
     5521 gcaaccgtgg cagcagttcg gtgtaaccac tgatgagacg ctacgcccgt cgcaggcagg
     5581 gcaaaccatt gagaacctgt ttgccatcgg ttcggtgctg ggcggatttg atcccatcgc
     5641 ccaaggatgc ggcggcggtg tttgtgccgt cagtgcttta catgccgctc aacagattgc
     5701 ccaacgcgca ggaggccaac aatgaacgac accagcttcg aaaactgcat taagtgcacc
     5761 gtctgcacca ccgcctgccc ggtgagccgg gtgaatcccg gttatccagg gccaaaacaa
     5821 gccgggccgg atggcgagcg tctgcgtttg aaagatggcg cactgtatga cgaggcgctg
     5881 aaatattgca tcaactgcaa acgttgtgaa gtcgcctgcc cgtccgatgt gaagattggc
     5941 gatattatcc agcgcgcgcg ggcaaaatat gacaccacgc gcccgtcgct gcgtaatttt
     6001 gtgttgagtc ataccgacct gatgggtagc gtttccacgc cgttcgcacc aatcgtcaac
     6061 accgctacct cgctgaaacc ggtgcggcag ctgcttgatg cggcgttaaa aatcgatcat
     6121 cgtcgcacgc taccgaaata ctccttcggc acgttccgtc gctggtatcg cagcatagcg
     6181 gctcagcaag cacaatataa agaccaggtc gctttctttc acggctgctt cgttaactac
     6241 aaccatccgc agttaggtaa agatttaatt aaagtgctca acgcaatggg tatcggtgta
     6301 caactgctca gcaaagaaaa atgctgcggc gtaccgctaa tcgccaacgg ctttaccgat
     6361 aaagcacgca aacaggcaat tacgaatgta gagtcgatcc gcgaagctgt gggagtaaaa
     6421 ggcattccgg tgattgccac ctcctcaacc tgtacatttg ccctgcgcga cgaatacccg
     6481 gaagtgctga atgtcgacaa caaaggcttg cgcgatcata tcgaactggc aacccgctgg
     6541 ctgtggcgca agctggacga aggcaaaacg ttaccgctga aaccgctgcc gctgaaagtg
     6601 gtttatcaca ctccgtgcca tatggaaaaa atgggctgga cgctctacac cctggagctg
     6661 ttgcgtaaaa tcccggggct tgagttaacg gtgctggatt cccagtgctg cggtattgcg
     6721 ggtacttacg gtttcaaaaa agagaactac cccacctcac aagccatcgg cgcaccactg
     6781 ttccgccaga tagaagaaag cggcgcagat ctggtggtca ccgactgcga aacctgtaaa
     6841 tggcagattg agatgtccac aagtcttcgc tgcgaacatc cgattacgct actggcccag
     6901 gcgctggctt aaactccttt ctgatgcccg gtaagcatgt ggttaccggg catttttgcg
     6961 tacacgattc cgtgcccaat gtatgcgttg caacgcagtg aaaattcctc tgaaaacgtc
     7021 tcgcaaaggc tgaaactggc agatgtcaaa ggcctgggat aaccgtaatg tcgcgtcatc
     7081 ataaatatca ggtgacggac aaccatgacc gaatcaacaa cctcctcccc gcatgatgcg
     7141 gtatttaaaa cctttatgtt cacacccgaa accgcacggg attttctcga aatacatttg
     7201 ccagaaccac tgcgcaagct ttgcaacctg caaaccttac gcctggaacc cactagtttt
     7261 attgaaaaaa gtttacgcgc ttactactcg gatgttttgt ggtccgtgga aaccagcgac
     7321 ggtgacggct atatctactg cgtgattgaa catcaaagct ctgcagaaaa gaatatggct
     7381 tttcggctaa tgcgctatgc cactgccgcc atgcagcgtc acctggataa aggctatgac
     7441 agagttccgc tggtggtgcc attgctgttt tatcatggcg aaacatcgcc ctacccgtac
     7501 tcacttaact ggctggatga gtttgacgat ccgcaacttg cccggcagtt gtacaccgaa
     7561 gcttttccgt tggtggatat caccatcgta cctgacgatg agatcatgca acatcggcgt
     7621 atagctctgc ttgaactgat tcaaaagcat attcgcgacc gcgatttaat cggtatggtc
     7681 gacaggatca ccacgctttt ggttaaaggc ttcactaatg acagccagct acaaacactg
     7741 tttaattatc tgctgcaatg cggcgatacc tcccgtttca cccgttttat tgaggagatt
     7801 tccgaacgtt caccactaca aaaggagaga ttgatgacta ttgctgagcg gctacggcag
     7861 gaaggacacc aaattggctg gcagaaaggt atgcatgaac aagccattaa aattgctttg
     7921 cgcatgctgg aacagggcat tgatcgtgac caggtgctcg cggccaccca gctaagcgaa
     7981 gccgatctgg cagcgaataa ccactaatta acacaggccc acagccgatc cccatgggcc
     8041 tttgatatca ataactacct tttatgcgtg gcccattttt gcctgattta aacatcgcca
     8101 gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac gccaacagcg acaaagttcg
     8161 ctccccacgc caggcattgc tgcgccatat caggagccac agccagaaaa ccagccgctt
     8221 tacccgcagc acggatccgc cgaatgctgg tttcaataat tcgctgcact tccgggtgcc
     8281 cggcgttatc cgggtagccc aacgacgcag aaagatccgc tggtccaata aacacgccat
     8341 caatcccttc gacgtcgagg atctcgtcca ggttatccag tgccgtttta ctttccacct
     8401 gcactaacag acaaagcgaa tcgttgactt gcgccatgta attctcaatg cgtccccagc
     8461 gcgcagcccg tgccacactg gccccaacac cacgctcacc gtagggagga tagcgcgtgg
     8521 cagacgccac ctgacgagcc tgatcggcag tatcgaccat cgggatcaat agagtttgcg
     8581 cgccaatatc cagaacttgt ttaatcagcg atttactgcc ttccaccgga cggatcacgg
     8641 gttggctggc atagggcgct accgcctgta gctggtgata aagatcctga atggtgtttg
     8701 gcgcgtgctc cccgtcaatc agcaaccagt cataaccgga agtagcggca atttctgcca
     8761 tataagaagt agtcgagctt aaccacagac caatttgcac ttcgcccttg cgtaaacgtt
     8821 ctttaaaggg attggttaat aatgcgttca tcgtgttcct taacctattt aatgatgtgc
     8881 cacgtcggtc tgcgcaacag tacgatttac acgcagagtg aaaataatca gcgaaccgat
     8941 gaccgccacc gctgccagtg tcagtaatcc tgccgcatcg ctggcaaata acgtttctgc
     9001 tttcacgcgc aggatcgggg caataaaccc gcccactgca ccaaacaggt ttacaaaacc
     9061 aatccccgca gccagcgccg taccggaaag cagttgtgtc ggcatcgtcc agaacaccgg
     9121 ctgcacggca ataaacccga tagctgcaac acacagcgct acgatcgcca ttactggaga
     9181 aagcagcccg gacagaccaa taccaatgcc cgccgccagt aatgtcagcg ctgcgacatt
     9241 acgccgttcg ccggttttat cggaatagcg cggaataagc caggtcccaa acaaggccgc
     9301 aacccacgga atggcggtga ccaccgacgc tgtaaagccc acttttgttc ccagcaatgc
     9361 cgcaacctgg gtcggcagga agaaaattaa tccgtaaacc gccacctgaa tggtcaggta
     9421 gataatcgct agttgccaga ctcggccatt acgcagcgca tcgctcagcc gagaagtcac
     9481 tttctgttgt tcttcacttg ccagttgatt gataagcaac gttttttctt gtttactcag
     9541 aaaacgtgcc tgctccggtg tgtcatcaag ccaaaagaat gtgaataccc cagcaccgac
     9601 tgccaacaat ccttcaatca caaacatcca gaaccagccg ggatgcccca taaatccatg
     9661 catctccaac agcgcgccgg aaagcggtga tcccagtgtt aacgccagcg gtgcgcccat
     9721 atagaacagc cccataatgc tggcgcgatt acgctgcgga aaccattgcg aggtgagata
     9781 aatcatacca gggaaaaatc cggcctccgc agcaccaagc agagtgcgaa ctatcagaaa
     9841 tttcgcttca gtatcggccc atgccatggc tgccgaaaga aatccccaca gcagtgttgt
     9901 cgtaccaatc caggttctgg cccccagttt gcgcatcaaa agattcgccg gaacacccag
     9961 aaacgcatat accacaaaga aaatgcctgc tcccagcgca taagcttcat tactcaaccc
    10021 ggtatcaatc tggtaggtct gtttggcaaa accgatattc gaacggtcga gaaacgccag
    10081 cacatacagc gccaacataa acggaattaa acgcacacgg tttttcttca ccacggcgtc
    10141 aagcaaagcg gtgctcataa aaagctcctt agaatatgtg ggcgttaccg ttgtcacagc
    10201 aacgcccgga gtgattagtg gctgtagggg cgtttcaaat tgcagtcacg attgagttcg
    10261 acgccaaaac cgggtttatc aagcactgat ttatgaatac gaccattcac cggaaccggc
    10321 tcattgagca gaatcgggtc aaactgcgga cgcatcgttg aacaatccgg gctggtcatc
    10381 aggaattcgc tgaatggcgt attggtgaag gtgatcaccg catggtgaga gtaaacagac
    10441 gaaccgtgcg gcaccaccag ttgccccctg gatttggcga ttgcggcaat ttccaccagc
    10501 gtggttaaac caccgcacca gccaacatcc ggctgcataa tgtcgatacc ggtttctgaa
    10561 agcgtacgaa aagattgcag tgtgccgtgg tgctcaccgc tggtgaccat cattccgact
    10621 ggcgcgttgc gtttcagttc gcgataacct tcatactgct gtggcggcag gcactcttcg
    10681 atccatttca ggttataggg cgcgcaagcg tgggccagtt tggtggcata gttcacgtcc
    10741 tgactcatcc agcagtcgag cattaaccag aaatcctcac cgcatttttc acgcatatcc
    10801 gcgaccataa cggcatcttt gcggatcccc gcatcgccat catgtggccc ccagtgcgtc
    10861 ggcattttgc caccgataaa gcccatctct tttgccagat ccggacgcgc gcctgtggcg
    10921 tagaactgaa tctcatcacg aactgcgccg cctaaaagtt tataaaccgg aagcccgacc
    10981 actttgccga acagatccca cagagccaga tcgacacagg aaatcgtgtt catcaccagg
    11041 ccaccagagc cggagtaata cagggtggca ttgagcattt gatcatggat cagtttgata
    11101 tcactgacac atttaccctc aatgaaacgg ttaagatgtt tttcgacaat aaaacagccc
    11161 atttcaccgg ctgtcgaaac agcgaatccg gtctggccgt tttctgcttc gacttcaaca
    11221 atcaacgtgc caagaacgtt aatgccaaat gactggcgtg actgctcgta atcgcggtat
    11281 ttactcatcg gggtggcaat atgatcgtca atccagtgat tcgccccctg gtcgtgataa
    11341 tcaccgcgcc agcacctttt tctgctgtcg caccgccagt aaaccaggcg cgaacctgtt
    11401 tgatttttgg tagggtcatg atgttctcca ttgttatgag gcctgtaagt caaagggact
    11461 tttccatccc aacagacgtg aaatatccct ggcgcaggca atggccttgc ccgccagata
    11521 atcacggtat tcttcattga tttgtaagcg ggtaccgacc accgagatcg cagcggtaag
    11581 ctcgttattg gcgttaaaca ccggcgcagc gacacaacgg acatcggggt aatcttcgcc
    11641 gttgtcatag ctccagccct gacggcgaat acgcgccagt tcttcgtgaa gttgctgtgg
    11701 atgagtaatc gttgtgggtg tcgcctgctc ccagaccagc ccttcgataa tactttgctg
    11761 taccgctgca ggttgccagg caagcaggca tttacctatt ccggagcgat aaagcgaaag
    11821 gctttttcct tcatgggaac gcacgctgat agtggctgat gactccactt ttaaaatgta
    11881 ataagcgctg ccgttatcaa taatccccag gtgacataac agcccggtgg tatccatcag
    11941 ttgcgtcaaa cgcggtcgag ccagttcccg gagatccatc ttgcttaacg catgaccaga
    12001 aagctccacc agcctggtcc acaaacaaaa attttcctgg tgatcaaggc taagaaaacg
    12061 ctgacgcctg agttcattaa gcagcaaata ggccgtgctt ttagggattc ccaatgtgtc
    12121 aatgatcgtc gcagcactac aggggccaat gcgggcaatc aaattgagaa tatcaatagc
    12181 ccgtgtcagg gcgggaactt tgcttgattc caacatactg gactccagtc ttagatactg
    12241 gaatcagtct cgttgctcag ggggagtaaa attgtgaagc gcatcaaatc ccgctcctcg
    12301 tccagcggac tggagaaatt acgcacaaat ttgtgaatca gtgcacggat tttttcttat
    12361 tgagtacaac aggttggaca agttttgccg gagggggaat gtgccggtgc agtaaaccgg
    12421 cacagagtgg cggaatcaga cagataaggt catggactca acaacctcaa tccagccatg
    12481 ctcactggcg atatcctggc cgtttaacca acggcgcagc atattcagtg ccatcattgc
    12541 gcacacttcc tgacggatag ccaggctgta gcgtgtagtg ctgaaacgca cacgcagagc
    12601 gaaagtgccg tctggcgtgg ctagcgcaaa gttgagatgc tcgttctcga aacccgaaac
    12661 agccagtgcc agcccggcaa aatggttggc ccgccgttct gtaatccagt gcgcagtttg
    12721 cgccagggtt tcttcctgtg aaggaaccac ttcacacgcc agcaatggag cacctgcgcg
    12781 agaaagttgc aaagccaata aaccaccggt gaattgctcg ctcaacgtca ggctgaactg
    12841 gcggttttgc aattcgcgac tgatctgcgc gggcagtcct tcggtgcctt cgaaaatcac
    12901 gctctgtccg gcaacacgtt taacgtccag ccacagtttt tccatcgcct gttgctcgct
    12961 tgccggtccg gtgagtttca gttcgatgat cggcattgag gagcgatagc ccattgttac
    13021 gcccggcggc agttgtagag tgtccaggct ttgtgccaga tcgctttccg aacgaccaaa
    13081 ggtagttaaa cgcagacaaa ccggcgactg tggtaaagaa aaacgctcgc gcaggcgcgg
    13141 caggatttcg tgctcgacca tcaccttaaa ttctgacggt acgccgggag taaagaacat
    13201 caggcaacga ttaagccgca cggcaaaacc acaggcggtg cctaccgggt tattgataaa
    13261 ttcagcactg gcaggcagct ccgcttgttt acggttgctc ggtgccatta ctcgtccacg
    13321 ttcgtggaaa tagcgttcca tctctttgag ccaggcttca tgcagcacca ggccttcacc
    13381 ttttgctgtc gcagcggcga gtgcacttaa atcatcactg gtcggtccca gcccgccgtt
    13441 aacgatcagc acatcggcgt gctgactacg ttcgcgaaga atggtgacta agtcatcaag
    13501 gttatccccc accgtattgc ggcgagataa tggcaacccc tgatgaaaga aaaaatcggc
    13561 cagccaggca gcgttagtgt caacgatttg cccgtgtaac acttcatccc cggtggataa
    13621 catttccact tttaacatgg ttatctcctg cttttgttat cgaatcacta taacgcaaag
    13681 ggagataagg cgtagtaaga aactgaatgg cgcgacagaa tgccgcgccg gaagggatca
    13741 gaaactggcg ttaaccccaa catacgggcc gtcagccacg gcgttgtcgc ggttaccgtc
    13801 tttacccgac agattcaggt agcgataacc cgcctcaata ctgactggat gcataatggt
    13861 gtaacgcgcg ccagcattcg cttcctcata acttttaata ccgctggaga gtgaatccgg
    13921 agagtagtaa tactcgccaa acaaacggaa gctgttgcca attttccact gcaaaccacc
    13981 tcctaccgct gcggcataac cttcatcgcc gtaattcggg ttggtgtaaa cgccttttcc
    14041 gccaacggtc gccattaacg gcccgagagg cagattcaac cccagcccca cgccagcgac
    14101 gtcaccgtcg tcgtcgttat gtgtccagtt accgcttaac gccaggcccg tcgattcagt
    14161 cccaaaaccg acaccaatat tggtgtattc tttacccgcc tgaccgctga tgctcattgc
    14221 attgaccgat gcagaaacca acagcattcc ggccagacct gctagcgcaa tttttttcat
    14281 tatccccttt cccacaagca attaatcaaa attcccaaag ctgcgggatt gtactgcctg
    14341 gcacagagga ttcaatgagc gaaaaaaggg atctagtcac ttattgtaac taaatacttc
    14401 ttaaataatt aaacgccgaa aaaataaaca cactttaccg atacacacaa aaataataat
    14461 aagtgcagtt atcatcgcaa aagagattac tgtcactttc acatatttct gagttaccgt
    14521 attctcatgg tttcttttta tttataagga atacccctgt gcgacaacgg actattgtat
    14581 gccctttgat tcaaaatgat ggtgcttatt tgctgtgcaa aatggccgac gatcgcggcg
    14641 ttttccccgg tcaatgggcg ctttcgggtg gcggcgtgga gtctggcgaa cgaattgaag
    14701 aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg acagaaatca
    14761 cgccgtggac cttcagcgat gatattcgca ccaagacgta tgcagatggt cgcaaggaag
    14821 agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa gtgaaaataa
    14881 acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggtgcat tatgatttaa
    14941 atgttgccac ccgaaaaacg ttacgtttga aaggtcttct ttaacaatga tgacagccat
    15001 cactggctgt cagattgaaa atttagtggt taacgaattc cccatccaga taaactttgc
    15061 ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatgtc gcatcacgct
    15121 tatttttagc aatatatgtc aggcaatgat tatgggtgaa aataacgata tttttatcag
    15181 gcgctttgct ttgtaagtcc ttaattgcac tataaatctc attaccgcac tgaagaagtc
    15241 gtttatctac cgtcagtttt ttacccgcgg aaaaccaggt cgccgactga atggtccgga
    15301 cggtattact ggaataaaga tcgaaatcag ggatgtcagc gctaaaagcg ttgcccagtt
    15361 cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt atctgacaag cattgattgg
    15421 ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccgggtgc tgctgtgcca
    15481 gtctggccaa tgttttattg tcgatacgtg gcaaaccatt gctactccag gcggcatgag
    15541 tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gaaatatttt tttgacttca
    15601 atgatgagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc aaccagacaa
    15661 ttaccgtgaa attgagcgac atttctggcg ataattagca gttggtgtaa tattaaaaat
    15721 cctacgatgt cggcaaaatg cctcaaaatt ttgccaaatg caaagcctaa ataagaaaaa
    15781 atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt taatattcta
    15841 tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag caaatataag
    15901 tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct ttttcgcgac
    15961 cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca
    16021 caaccggtcc gaagaatcag gcgcttgagc aagccttttg ccagttgacg ggaaatcagc
    16081 atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa
    16141 ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga
    16201 tttccttgtt gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca
    16261 cgcctgaagc gatcgagtca gccattacgc cacgcactaa agccatcatt ccggtgcatt
    16321 atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt tacggcatcg
    16381 cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga catattggcg
    16441 caaaaggtac cgctattttt tcatttcatg ccattaaaaa tattacctgt gcggaaggtg
    16501 gcctgattgt aactgataat gaaaaccttg cccgccagct acggatgctg aaatttcacg
    16561 gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag gctgaagtct
    16621 taacaccggg ctataagtac aatctgaccg atattaacgc cgcgattgcc ctgacacagt
    16681 tagcaaaatt agagcacctc aacacccatc ggcgcgaaat tgcccagcaa tatcagcaag
    16741 cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac gttcacgcct
    16801 ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga
    16861 tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcgcc gctcacacac
    16921 aaaaatatta tcgcgagcgt tttcccacgt tgtcgttacc gaataccgaa tggaatagcg
    16981 aacgcatctg ttctttgccg ctgttcccgg atatgactac cgccgatgcc gaccgtgtca
    17041 tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac cctgttaaga
    17101 aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc
    17161 gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg attgatgacg
    17221 gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag aacagccata
    17281 ttgtgtctat tttgcttaac cgcaattacg ggcaacattc agcgattatg gcgggattca
    17341 gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat ccgccagaag
    17401 aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg actgtacgcc
    17461 agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa gatgattaac aggcttattc
    17521 agcgcaccac cggcaaagcg atgggtgact acggttgtat gctgcgcgcc tatcgccgtc
    17581 atattgtcga tgcgatgttg cactgccatg aacgtagcac ctttatcccg attctggcga
    17641 atatcttcgc ccgccgtgcc attgaaattc cagtacatca tgccgagcgt gagtttggtg
    17701 aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg acctgcctta
    17761 ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt ggaggtttta
    17821 gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag
    17881 gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt atcggcatgg
    17941 gattgctcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt
    18001 ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt
    18061 tgcctaccac gatatgggat gcctcggtat tgaagctctg ctggctgccg gttacgaaat
    18121 tagcgccatt tttacccata ccgataatcc cgctgaaaaa gccttttatg gttcggtggc
    18181 tcgtctggcg gcggaaagag gcattccagt ttatgcgccg gataacgtta atcatccgct
    18241 gtgggtggaa cgcattgccc aactctcacc agaggtgatt ttctcttttt attatcgcca
    18301 tcttatttgc gacgagattt tgcagctcgc tcccgcaggt gcatttaatc tgcacggttc
    18361 gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac
    18421 ggaaacgggc gttacattgc accgaatggt aaaacgtgcc gatgctgggg ccattgtggc
    18481 gcaactgcgc gttgccattg cgccagacga tatcgctatt acgctgcatc ataaattgtg
    18541 ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg gtaatattct
    18601 ggaaattgcc cagcgcgaaa acgaagccac ctgttttggt cacagaacgc cagatgacag
    18661 tttccttgaa tggcataaac cggcatccgt actgcacaac atggtacgtg ccgttgccga
    18721 tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg
    18781 tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg cgccactgct
    18841 gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg acggcattac
    18901 tatgcagggc tcgcaattag cgcagatgct gggcctggtg caaggttcac gcttgaatag
    18961 ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt
    19021 tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct
    19081 ggatattggc agcgatgcga taagccgttt tctgaatcat ccgcattttc actttgtcga
    19141 aggcgatatc agtattcatt ccgaatggat tgagtatcac gtcaaaaaat gtgatgtcgt
    19201 cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt
    19261 tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgttaagt accgtaagcg
    19321 aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat acttcgatga
    19381 ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtgtc
    19441 aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt tacagttcac
    19501 cctcttccgc ccgtttaact ggatgggacc acgactggat aaccttaatg cagcgcgaat
    19561 tggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt caccaattaa
    19621 gctaattgat ggcggaaaac aaaaacgctg ctttactgat atccgggacg gtatcgaggc
    19681 gttgtaccgc attatcgaaa atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg
    19741 caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg cgagcttcga
    19801 aaaacatccg ctgcgccatc acttcccacc gtttgcgggc tttcgtgttg tcgaaagtag
    19861 cagctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc
    19921 ccgccgctgc ctggactggg aaccgaaaat tgatatgcag gaaaccatcg acgaaacgct
    19981 ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtcggctta
    20041 cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccacgtct gctggaaatc
    20101 ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc
    20161 cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaacgcg
    20221 gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg taaagagatt
    20281 ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt cggcctgcac
    20341 gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca aacaatgatc
    20401 gacgatattg cacgcggtct tcgcactctg gaagagatta tcggtcaacc ggtaacctgt
    20461 tctgccgctg cgggctggcg tgccgaccag aaggtgatcg aagcaaaaga agcgttccat
    20521 ttgcgctaca acagcgattg tcgtggggcc atgccgttcc gtccactgct cgaatcagga
    20581 aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg
    20641 gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg agataaaggc
    20701 acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca taattttgtg
    20761 gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag cgaactgtta
    20821 tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg
    20881 ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac cttatcggcc
    20941 tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg ctctggcaac
    21001 ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc gactggattg
    21061 tgccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca
    21121 gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc gttatctttg
    21181 cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcgataaac
    21241 gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat gccatcggca
    21301 cttatgccgt gctcgatccg tttattgcat tctggctggt ggcgggaatg tgcagcttct
    21361 ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta ctgctgggaa
    21421 tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa
    21481 gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt tacggctggc
    21541 tggcggttat cagttgcgta ctgacggttc tcccctgggg actggcgata gcgcagcggg
    21601 agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt gcactggatg
    21661 atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc ggtagcctgc
    21721 cgtggctggg attactcccc ggtgcactgt acacaggctg gaaaaaccgc aagcattccg
    21781 caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc gtcgctaaag
    21841 gtaaattgcc cacctatatt ctttcctgct ttgcatctct ggcaatgctg atggcgcatt
    21901 acgctttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc tggattaaca
    21961 tcgcttttgg cttcactggc attattgcca catttgtggt ctccccgtgg ggaccaatga
    22021 acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt
    22081 cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc tggtcttttg
    22141 ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtca
    22201 tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc
    22261 gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg
    22321 atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat tatccggatg
    22381 cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg
    22441 aggggattat tacactcgtg ctttcggttg accgcgatga agatatcaac agtctcgcca
    22501 ttccgcccgc agatgccatc gatcgtcagg agcgtctggt gctgattcag tatcgtccca
    22561 aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc agttgtgtca
    22621 gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg tgctgtggct
    22681 gggactggcg ctggcttgtc ttggtcttgc catggtgctc tggctgctgg tcttgcagaa
    22741 cgtaccggta ggcattgcct acccgatgtt aagtctgaat tttgtctggg tgacgctggc
    22801 tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat
    22861 tattggcggc attgtgatcc tcgggagtac ggtgtaatgg gcctgatgtg gggattattc
    22921 agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt ttgcggcgag tcatctgccg
    22981 ccgatgacgc acttatggga ttttatcgcg gctctgttag cttttggcct ggatgcccgg
    23041 atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta taaaacgcta
    23101 cataaacttg cgctaagcaa agcctatgcc ttgctcagta tgagttatgt gctggtatgg
    23161 attgcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc actactggga
    23221 gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccacgacaaa acaacgttac
    23281 tgagttttcc ctgccacttt acactgccgt tcccactcat cggggctata actgcttgcc
    23341 gagaggactt tcaccgtatg cagcttttca cgattaatac aataaagcgc attctgcaaa
    23401 ggcgagagta aatcccccac tttcacggcg aagtcactct tcacttcggc gatcattttt
    23461 atcgcgccgc cagcatcgca gagcataagt acatgtctgt tatcttgttt attgcagcac
    23521 gattttttga ccagccattc cattgcatta tcctgtttgc taagagtttt cacatcaatt
    23581 tacagattaa cagataaacc tattatcaac ggcaatggaa tggcaaaatt gcgaagtgaa
    23641 actaatgatt tcgtaatcct ggcgttgcac ccactctttt aacgcctgac gtgaaatttt
    23701 aataccgccg tttttcagct ccggcggtaa agttagccag cgcaccggtt gttgaaaacg
    23761 agccagctta tctttcaccc attcgccaag atcaacggtt tgctggtcat actccaccac
    23821 cgctaccggt cgatgaccaa actccttgtc ggcaacgggg acgataaaca cctgcaaaac
    23881 cgcaggatgt gcagcaatta cgcgctcgac ttcttccggc tgaataccct ctccgccact
    23941 gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg ccattatgca tctcaccgcg
    24001 atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa accagttgcc cgttacgcca
    24061 gtaaccttct gccatactgg cagcccgcag ccacacttca ttattaacga ttttcacttc
    24121 ccgacccggc agcggcgaac caacgtctgc caggccgtcg gcttctttcg cacacaccgt
    24181 ggaggcaaac tcggtcagac catagccgca aaagcaacga atcccctgct cgcgcgcctg
    24241 ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac accgctttca gggaaacgga
    24301 actgcggtta accagcaaac gccagagttg tgttggcacc agtgaagcgt gagtacagcc
    24361 tgccagcatt tgctccaatg gctgtttatc acgcaccgtc atccgcgcac cagcgtataa
    24421 ccagcgccac ataattccct gaccggagac gtgaaacagc ggtaaagaga gcaaccagtc
    24481 atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc gcactggcaa gatgggcctg
    24541 ataagtatgg acagcggctt tcggcaaacc ggtagaaccc gaggtcaacg tcattgagca
    24601 cagacgcgtc ggctgccacg tagcggcatg tgcgccttca acccgctgaa tgtgcagcga
    24661 cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc gcaaattgca gcgtcagatt
    24721 gggcagcaat tcttcaagca acggttgcgg cagctgaggg ttcacgggca acacccgcgc
    24781 cccgcattgc agtaacgcca gccaggcgag cagcgtttgc ggcgtattcc acgcccgcaa
    24841 catcacgccg ctgccctcaa ccacaccctg cgccgcaaag cccgtagcta actcatcgac
    24901 gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta agacgtaagg cgatggcttc
    24961 tccccgcact tgccgccagt gacgccacgg ccagtcagag aagatcataa caactgctcc
    25021 agtacatcag cgtccactaa cggcagtggg ctccccggcc agcgacgaac ctgctgcgcc
    25081 tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg gcgttaacca ggcagcaatc
    25141 cgcgccagtt gcgttagacc taagctcgac tcaatggaag aactgatcac cgccgtcagc
    25201 cccagcgcat gcgccgcctg tacctgctcg cgtacttttt ccagactgcc cgtgagcgtg
    25261 ggtttgataa ctaccgcgcg cacgccctct tcagccacaa aggcaaaatc cggctcgcgc
    25321 aggctttcat cccaggcaat ggcaatgccg gtttcacggg caaacgctcg cgaatcatcg
    25381 cgggttttgc acggctcttc gagaaacgcg atgcggtggc gataatccgg gttaacatat
    25441 ttggcaaact gctgaccttt cagcggtgtc caggcgcgat ttgcgtcaag acgcaaatgc
    25501 agatccggaa ttgcctccag caacagatta accaccatgc cgtcgcgcac ggcttcgtac
    25561 aatcccactt tgaccttcgc cactttctcg cctggcatat cagcaagttt gaggatcaga
    25621 tcgtcaggat cgccattaca cagtggtgcc gcacggtaat tggccgcctg cgggagcgta
    25681 tctgccagct ccgccaatgc acagcttacg ccaaaggcaa cggaaggcat ctgcggtagc
    25741 tcgccatcgc ctgccagcca gttatttacc caagcaagca gcacaatttg cgcctcttcc
    25801 caggtttcct gactgaagcc cggcagtggg gagatctccc cccacccttc gcgctcgcct
    25861 tcacgcaggc aaacatacag cccgtcgcgg gtttttaacc gcctgtcgcg cagaaccatc
    25921 cccgcgtcca tggggatctg ccagcggtat acctgcgcgc tacgcattac ggattccgtt
    25981 taaatttgct gaaatcaggc tgacgtttct ggttgaaagc gttgcgacct tcctgacctt
    26041 cttccgtcat gtagaacagc atggtggcgt tgcccgccag ctcctgcaga cccgcctgac
    26101 cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag cgccatcggg ctgttttgca
    26161 gcatttcgcg gcaccaacgg acggtttctt tttccagatc cgccagcggt accacggtgt
    26221 tgacaaggcc catatccagc gcctgttttg catcgtactg acggcacagg aaccagattt
    26281 cacgcgcttt tttctgtccg acgatgcgag ccatgtagga agcgccccag ccgccgtcga
    26341 aggaaccgac tttcgggcca gtctgaccga agatggcatt atctgccgcg atagtcaggt
    26401 cgcacatcat gtgcagaacg tgaccaccgc cgatggagta gccagccacc atcgcgacaa
    26461 ccggtttcgg acaggtacgg atctgacgct ggaagtccag cacgttcagg tgatgtacgc
    26521 cggaatcatc tttatagccg ccgtaatcac cacgcacttt ctggtcacca ccggagcaga
    26581 acgctttatc gcctgcgcca gtcagaatga tcacgccgac gttgtcgtca taacgcgcat
    26641 ctgccagtgc ctggatcatc tcttttaccg tcagaggacg gaaggcattg cgcacctgcg
    26701 gacgattaat ggtgattttt gcgataccgt cggtggattt ttcataacga atgtcctcaa
    26761 aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaaag cattgcttca tcaggataaa
    26821 tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc agacttgcga taacgccagc
    26881 gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg acatggcagt cggcagccag
    26941 ttccgccgcc aaggcgcgga atttgctgtc acgttcacca cataaataat aaaacgcaaa
    27001 tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg gcgagagaag tcgcctccag
    27061 catggcggca agggttgcgc cattattgtt actgcgcagc gccaccagct cccggcgttg
    27121 atcgtcattg agcgaggcaa aaacaggctg ttgataccag tcggcaaata ccgccgttaa
    27181 cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa cgctgacgtt ccgcgcgttg
    27241 ttcagcattt tgcaacccag gatgcccgcc ttcgacaaca accccacaaa gccccggcat
    27301 cccctggcaa gccgccatca tcgccacccg tcccccaagc gagtacccca ccaaccagaa
    27361 gttaaggatg ttgtaactaa ccagggtttt acgcagtaag tcggtgacat catcaaatcc
    27421 atcgacgcta atcgccgccg aaccaccgtg acctgggaga taaacataca accgtgagta
    27481 gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa tcgccggaaa aaccgtgcaa
    27541 aaacaccagc cagggtaaac ctggtttgcc gtgttttgcc tgcgcgtgca ggatcataaa
    27601 tggcttacct gcgccagaag ttgctggagc gtttgcgcac catcggtgtc gttaaccacc
    27661 atttcaatta ccgtggtggt tggcgtgcgc caggcatcgg caagtgccgt ttcaagttcc
    27721 tgccagtttt gcggacgatg atatttcagc tcgaacatcg cggcggcgtg ctcaaaatgg
    27781 acgttttgcg gcatcagata gaaacgctcg cgctcgcttt tcggcgttgg cagcagcgag
    27841 aaaatttgcc cgccgttgtt gttcaccaca attaatatca gcggcgcgga aacctgacgc
    27901 aataacgcca gcgcgttgag atcgtaaagt gcggagagat cgcccacaat cgccagcgtc
    27961 ggtttgccgc ttgcccgctg aacgccggcg gcagtcgaga gcagcccgtc gataccgctg
    28021 gccccacggt tgctgtacac cgggtaacct gccggaagtt gcgaaagcgc atcaatcaga
    28081 cgtaccacca ggctgttacc gacaaacaat tgcccctgtt caggcagata gtcgctgatg
    28141 cgatgcgcca gttgcgcttc gccaaacgca tcgcgacggg caataaccgc ctgcattgcc
    28201 tgttccgcca ggcgcgggat ttcaacgcac cagggctggc gtttttctgc cggatgcagc
    28261 tccagccagt cggcaatatt ggcaattaag cggcgtccac gatggtgcgc cggatcaagc
    28321 cgcccttcga tgtcatccac aatccagtac tcttcgggtt cacagcttgc ctgccattgc
    28381 aggagccgtt tgcccgtcag gctgcttccc agttgcacca caatttgcgc ctgttgcagt
    28441 tcgctggtcg ctttggcatt gcctaaccag agatcggcac acggcagcgg ctgtccggtt
    28501 tgcgacagca cgtcgccaat cagcggccag ccaagagttt gcgcccacag tgcaactttt
    28561 ttgccctctt ccgcactcat gcgcccggca accaccacgc cgcgtttttg tcgccagaag
    28621 aaccagtcgc gctgtttttc actttccaga cgaggcgctt cacgcagcca cggtttgtcg
    28681 tcctgccacc agtcgcccag ccgctgttgc cagctaagcc cggtgtcgtc catttcgcca
    28741 tacagcggtt cggcaaacgg gcagttgata tggactccac cagaatgaag cgtaccgaga
    28801 gcatggtcga tggtagaaac cagccaacgt gcggggatat cctgggtcgg gcgcggcaat
    28861 gaaatactgt gcgtggggtg agaggcgaac attcccggct gacgaatcgc ctgattcgcg
    28921 ccgcagtcaa tcagctccgg cgggcgatcg gcggttaaca ggatcagttt ttctccggtt
    28981 aacccagctt caatcagtgc cggatagaga tttgctaccg ccgtaccgga ggtcacaatc
    29041 accgccaccg gctgcttgct gactttcgcc agccccagcg ccagatgccc caacccacgc
    29101 tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg ccgccgctaa cgttaacggt
    29161 gtagaacgcg agccaggggc gatacagatg tgtctgacgc cgtgacgcgt taatgcttcc
    29221 agaatgaccg ccgcccagcg tcggttaaat gcgcttactg acatgagttt gtccggtatc
    29281 aatattgcgg ctaagtataa ggagctacaa aaatagatta ttgatatgaa tcggtaatga
    29341 tgcgactcat tactattcca tctgtaataa agtacgcagc cctgccgctt tgttgtcgat
    29401 ttcctgccac tcttgctcgg ggtcggaacc acggacaatg cccgcgccag catataatcg
    29461 cacgacattg ccgctaattt ttgctgagcg cagggaaacg cagaattcgc tttgctgtaa
    29521 tgagagatag cccgcagaac cggcgtacca ttctcgggtg aacggttcgt gacgggcgat
    29581 aaactgtcgc gccagatcgc gcggtaagcc agcaactgct gccgttggct gcaactgatg
    29641 taaacagatc acattatccg ctttgttgag tgaagtccag atacagcggc gaagatgctg
    29701 cactttacgc agacgcagta cctgcggcgg taaaacatcc agcgtctggg tatcggcctg
    29761 taaccgttgg cagatatctt ccaccaccag catattctcg cgctggtttt tatcatccgc
    29821 catcagccac tcacctaact gctgcgcctg cttatcatca gggtgatttg ctaccgttcc
    29881 cgccagcgct tcagtacgca gcgctttgtc acgccgccgc cataaccgtt ccggtgaaga
    29941 gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa tggtagcaat tcagattcag
    30001 tcgacgactg gcagccatca tcgccgccgc gttgaccgga cttgcgaaat gcaggtcagt
    30061 tgcccgagcg agcaccactt tgtcgagctc accttcggcg atggttttcg ttgccagttc
    30121 gattaattgc gtccagcccg ttttgtccgg ccagtgttgt tctcgcgtgg tggttaaatg
    30181 taacccaggc aagggcttga tactcaccag tgtggcgata aattctcttg ccttaatcgc
    30241 atcgtgctga agggagcttt cgctgaatag cgtcagccgc agcgtggctt taccgccaca
    30301 gcgtcgccat tccaggcgtg gtaaaagtaa attgccctgc gacgggtcaa acgcattcag
    30361 cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga agaaaacgtt gtgcctggtc
    30421 caacgacgta aaacgggtaa tcgcgcccag gacggcagct tcttcatcac cattacgttg
    30481 ttgccagtag aattgcgggt atgtttgctg actggccagc cagctcaagg catcaaaagc
    30541 gtctttgaga gggaaaggaa tatcgataac ccgaatgccg ggtgtcgccg gaatctcttg
    30601 cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt gattgcacgc ggacctctcc
    30661 ctgttaaaag cctcacatta tacggggtac tacaaaaaaa atgcagtacc ccggtgtagg
    30721 gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc ccaacggccg cgcccacacc
    30781 aattccttgc cagggttttt cgtggacgta gtcatcagca cgataaactg cctgcttcgc
    30841 ccgatagtaa taactgtctg aagcctggct aacccgtttt ttcacatcat ccagcgcttt
    30901 ttctgcacgc gctttcagct caacatattt ctgatcggcg ggatcgccag aggagcggag
    30961 cacctcttcc agtgtttcac taagcagcgt caggtcgtca tcgatacgtg tatcaccaaa
    31021 ctgattagac attctcgttc tccatttgcg taaaacctgt cactaactat agacaagggt
    31081 tgttgattac gcctgaatta cctcgcgtgc catcccaatg tgtgggatac catcttcttc
    31141 atagacctcc gtcacaggga taaaaccgaa actctggtag aagttttgca gatgcgcctg
    31201 cgccccgagg taaacaggct tatcaggcca gtgatgcgtg caggtgtcga gggttttact
    31261 catcagttgc tgccccactt tttcgccacg caacgcttca ctgacaatca cccgaccaat
    31321 aacgaccggc tcaagatcat catcactttt cagaatcctc gcatatgcca ccagttcatc
    31381 atttttccaa ccgagaatat ggcgattatc ccccgtcaaa tcatcaccat cgatatcctg
    31441 ataagggcaa ttctgctcga caacaaacac cgcgcaacgc agttgtaata aggcataaag
    31501 ctgggaaacc gataattcag agtgatgcag atcttaccat tcaatcataa tgctctcctt
    31561 ttagatgtat gccttatact agccggaata ttttttgaaa cgtgatgaac tcatggaatt
    31621 aattttttta ggtacttcag ccggtgtgcc aacccgcacg cgcaatgtca cggcaatatt
    31681 gcttaacctg caacatccga cccagagcgg actttggttg tttgactgcg gtgaaggcac
    31741 ccagcatcag ctactgcata ccgcctttaa ccctgggaaa ctggacaaga tttttatcag
    31801 tcaccttcat ggcgatcatc tctttggttt acccggcttg ctgtgcagtc gttctatgtc
    31861 aggcattatc caacccttaa cgatttatgg tccgcaaggt atccgtgaat ttgtggaaac
    31921 cgcgctgcgg attagcggct catggaccga ttatccgctg gaaattgtcg aaattggcgc
    31981 tggcgaaatt ctcgatgatg gcctgcgcaa agtgaccgct tatccgatgg aacacccact
    32041 ggaatgttat ggctatcgta ttgaagaaca tgataaaccg ggtgcattaa atgcccaggc
    32101 attaaaagct gctggcgtgc cgcctggccc actgtttcag gaattaaaag cgggcaaaac
    32161 aatcacgctg gaagatggaa ggcagattaa cggcgcagat tacttagctg ctccagtgcc
    32221 aggtaaagcg ctcgctattt tcggcgatac cggcccctgc gatgccgcac ttgacctggc
    32281 taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat ataaccatgg aagccaaagc
    32341 caatagtcgc ggccatagct ctacacgcca ggctgcgaca ctagcccgtg aggctggagt
    32401 cggcaagcta atcattaccc acgtcagctc gcgctatgat gacaaaggtt gtcaacacct
    32461 gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg gcgaatgatt tcaccgtgtt
    32521 taacgtttaa ctc
//
LOCUS       DAJTYZ010000036        31793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000036 DAJTYZ010000000
VERSION     DAJTYZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31793
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.036"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1132
                     /gene="ypdA"
                     /locus_tag="OO596_001607"
     CDS             <1..1132
                     /gene="ypdA"
                     /locus_tag="OO596_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6717492.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1147..1881
                     /gene="ypdB"
                     /locus_tag="OO596_001608"
     CDS             1147..1881
                     /gene="ypdB"
                     /locus_tag="OO596_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6717493.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1894..2751
                     /locus_tag="OO596_001609"
     CDS             1894..2751
                     /locus_tag="OO596_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6717494.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OO596_001610"
                     /pseudo
     CDS             complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OO596_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OO596_001611"
     CDS             complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OO596_001611"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6717495.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OO596_001612"
                     /pseudo
     CDS             complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OO596_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7411..8658)
                     /locus_tag="OO596_001613"
     CDS             complement(7411..8658)
                     /locus_tag="OO596_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6717496.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8680..9006)
                     /locus_tag="OO596_001614"
     CDS             complement(8680..9006)
                     /locus_tag="OO596_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6717497.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OO596_001615"
     CDS             complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OO596_001615"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6717498.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10395..11651
                     /locus_tag="OO596_001616"
     CDS             10395..11651
                     /locus_tag="OO596_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6717499.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11766..12092
                     /locus_tag="OO596_001617"
     CDS             11766..12092
                     /locus_tag="OO596_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6717500.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12233..13471)
                     /locus_tag="OO596_001618"
     CDS             complement(12233..13471)
                     /locus_tag="OO596_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6717501.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13807..15009
                     /gene="nupC"
                     /locus_tag="OO596_001619"
     CDS             13807..15009
                     /gene="nupC"
                     /locus_tag="OO596_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6717502.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15059..17247)
                     /locus_tag="OO596_001620"
                     /pseudo
     CDS             complement(15059..17247)
                     /locus_tag="OO596_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17456..17531)
                     /locus_tag="OO596_001621"
     tRNA            complement(17456..17531)
                     /locus_tag="OO596_001621"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17496..17498),aa:Ala,seq:ggc)
     gene            complement(17571..17646)
                     /locus_tag="OO596_001622"
     tRNA            complement(17571..17646)
                     /locus_tag="OO596_001622"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17611..17613),aa:Ala,seq:ggc)
     gene            17867..18226
                     /locus_tag="OO596_001623"
     CDS             17867..18226
                     /locus_tag="OO596_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6717503.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18228..18620
                     /locus_tag="OO596_001624"
     CDS             18228..18620
                     /locus_tag="OO596_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6717504.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="OO596_001625"
     CDS             complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="OO596_001625"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6717505.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20346..20421
                     /locus_tag="OO596_001626"
     tRNA            20346..20421
                     /locus_tag="OO596_001626"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20379..20381,aa:Val,seq:tac)
     gene            20467..20542
                     /locus_tag="OO596_001627"
     tRNA            20467..20542
                     /locus_tag="OO596_001627"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20500..20502,aa:Val,seq:tac)
     gene            20589..20664
                     /locus_tag="OO596_001628"
     tRNA            20589..20664
                     /locus_tag="OO596_001628"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20622..20624,aa:Val,seq:tac)
     gene            20669..20744
                     /locus_tag="OO596_001629"
     tRNA            20669..20744
                     /locus_tag="OO596_001629"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20702..20704,aa:Lys,seq:ttt)
     gene            20775..20900
                     /locus_tag="OO596_001630"
     ncRNA           20775..20900
                     /ncRNA_class="other"
                     /locus_tag="OO596_001630"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21020..21361
                     /locus_tag="OO596_001631"
     CDS             21020..21361
                     /locus_tag="OO596_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6717506.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(21352..22278)
                     /locus_tag="OO596_001632"
     CDS             complement(21352..22278)
                     /locus_tag="OO596_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6717507.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            22368..23366
                     /locus_tag="OO596_001633"
     CDS             22368..23366
                     /locus_tag="OO596_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6717508.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23363..23581)
                     /locus_tag="OO596_001634"
     CDS             complement(23363..23581)
                     /locus_tag="OO596_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6717509.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23583..25598)
                     /gene="ligA"
                     /locus_tag="OO596_001635"
     CDS             complement(23583..25598)
                     /gene="ligA"
                     /locus_tag="OO596_001635"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6717510.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25669..26655)
                     /gene="zipA"
                     /locus_tag="OO596_001636"
     CDS             complement(25669..26655)
                     /gene="zipA"
                     /locus_tag="OO596_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6717511.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            26885..27646
                     /gene="cysZ"
                     /locus_tag="OO596_001637"
     CDS             26885..27646
                     /gene="cysZ"
                     /locus_tag="OO596_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6717512.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            27831..28802
                     /gene="cysK"
                     /locus_tag="OO596_001638"
     CDS             27831..28802
                     /gene="cysK"
                     /locus_tag="OO596_001638"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6717513.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29186..29443
                     /gene="ptsH"
                     /locus_tag="OO596_001639"
     CDS             29186..29443
                     /gene="ptsH"
                     /locus_tag="OO596_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6717514.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29488..31215
                     /gene="ptsI"
                     /locus_tag="OO596_001640"
     CDS             29488..31215
                     /gene="ptsI"
                     /locus_tag="OO596_001640"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6717515.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31256..31765
                     /gene="crr"
                     /locus_tag="OO596_001641"
     CDS             31256..31765
                     /gene="crr"
                     /locus_tag="OO596_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6717516.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         7843 a         8383 c         7906 g         7661 t
ORIGIN      
        1 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcaga ccaagctggc
       61 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
      121 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
      181 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
      241 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
      301 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
      361 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
      421 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
      481 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
      541 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
      601 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
      661 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
      721 tgagcaggcc cgctttggtg acaagctgac ggttatctat gatattgatg aagaggtgaa
      781 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
      841 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
      901 acgcattgcg gtgcgagata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
      961 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
     1021 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
     1081 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
     1141 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
     1201 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
     1261 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
     1321 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
     1381 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
     1441 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
     1501 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
     1561 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
     1621 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
     1681 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
     1741 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
     1801 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
     1861 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
     1921 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
     1981 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
     2041 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
     2101 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
     2161 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
     2221 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
     2281 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
     2341 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
     2401 atgccgccgg gtaattcgca accgcgcggc gattttctct atcacagcat ttgtaactgg
     2461 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
     2521 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
     2581 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
     2641 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
     2701 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
     2761 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
     2821 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
     2881 atcccttcgt tagcacccag cgtcagcatg atcaccagtt caacttcgcc catttctgac
     2941 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
     3001 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
     3061 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
     3121 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
     3181 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
     3241 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
     3301 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
     3361 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
     3421 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
     3481 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
     3541 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
     3601 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
     3661 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
     3721 cataagatct gatcgaggct gttaaccatc gggatcatca actgggcgtt gccgaaactg
     3781 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
     3841 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
     3901 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
     3961 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
     4021 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
     4081 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
     4141 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
     4201 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
     4261 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
     4321 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
     4381 agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
     4441 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
     4501 taggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
     4561 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
     4621 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
     4681 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
     4741 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
     4801 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
     4861 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
     4921 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
     4981 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
     5041 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
     5101 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
     5161 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
     5221 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
     5281 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
     5341 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
     5401 gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg ttaagtgcac cgctccaccg
     5461 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgacacg
     5521 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
     5581 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
     5641 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
     5701 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
     5761 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg gtcatcaaag
     5821 gctttcccca tcacccgctg gtgagggaga acctgaaaag ctgtatcaaa ggtgacgcga
     5881 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
     5941 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
     6001 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
     6061 accggcagca catcaattgc cccttcgcgg gagatgctgc gcaccataaa tcccacttcg
     6121 tccatatgcg cacagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
     6181 gatcccagac catcaaatcg tacttctttt tgcaggcgat ccgcttcttc cagtaggatc
     6241 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
     6301 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
     6361 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
     6421 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtatcccgcg
     6481 gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc atgaccggtg ttgtgaccaa
     6541 aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc ggcgtcgtca acctgttagc
     6601 agcgtacgcc agggcgaatc gcggagattg ccgccagctg tgcttgcagg acaatctgat
     6661 agacgttaaa cagcgggtga gattcggcaa ttaccccttc gccattcacc agcaaggtgc
     6721 gcgtcatatc agagcagtac ccctgataga gcgcaccaaa atcaagagtg acaaactcgc
     6781 ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccacgc cagccgctgg
     6841 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
     6901 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
     6961 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
     7021 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
     7081 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
     7141 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
     7201 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
     7261 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
     7321 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
     7381 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttaccttt
     7441 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
     7501 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
     7561 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tcgcgccgac
     7621 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
     7681 aatccccata aaggcaagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
     7741 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agacccagac ctatcggcgg
     7801 aataacgata gcgatagaac gtgcagtgac cggtaatacg tggtcagtgg taaagctgaa
     7861 ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgcgg tcgccgcggc
     7921 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
     7981 accgccgttg atccagccgc caaagggggt aatgacgtag tacatcgcca gcatgacaaa
     8041 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
     8101 ctggattttc tggttcatcc acttcacaag gtaacccgcg acgatagaaa taatcagcgc
     8161 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
     8221 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
     8281 cagtttgcca ccgatagagt tcgccacaaa agcagcaaac atcggaatgg caaaaccaaa
     8341 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
     8401 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
     8461 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
     8521 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
     8581 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
     8641 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
     8701 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
     8761 ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc ggggtaactg ccacggagtg
     8821 gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg cgattctgga taccgtccgc
     8881 gccctgggtt tcaattttca cttcataacc tgcttctacc gccgcttctt ccagcgcctg
     8941 agcggccata aaggtgtgag ccaggcccat tgggcaggca cataaggcaa ttagtttctt
     9001 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
     9061 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
     9121 tgtgacgcag gtcggccttg tgagggggac acaaagaaaa attgaattaa ctgtatgatt
     9181 taaaagatta tcgggagagt tacctcccga tataacagaa aggatttaca gaatgtgccc
     9241 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag
     9301 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
     9361 gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgccag caatataaac
     9421 cccaccaaag gttcccagcg ttaatgccag attgccgcca aaacggccca taatgacgca
     9481 aaacagcgac aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     9541 atcttttggc ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa
     9601 attcaccagc ccaggaccag aaagcacgcg ttccgccgaa acatgaccaa tttccgcacg
     9661 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
     9721 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
     9781 tgttccggca ccgtaaaccg caataggttt accttcgacc ggttctgcgc caccaaactg
     9841 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
     9901 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
     9961 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    10021 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    10081 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    10141 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    10201 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    10261 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt
    10321 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    10381 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    10441 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    10501 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
    10561 ctatctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    10621 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc
    10681 cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    10741 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    10801 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    10861 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    10921 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    10981 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    11041 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    11101 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    11161 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    11221 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    11281 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    11341 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    11401 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    11461 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    11521 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    11581 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    11641 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    11701 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    11761 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    11821 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    11881 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    11941 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    12001 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    12061 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    12121 gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga
    12181 taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc
    12241 ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc
    12301 agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg
    12361 tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca
    12421 taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg
    12481 tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct
    12541 gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa
    12601 agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat
    12661 cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca
    12721 gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac
    12781 gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat
    12841 gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta
    12901 aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact
    12961 cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagtggtt
    13021 tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc
    13081 cctgcaacaa cgagacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg
    13141 ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac
    13201 gcggatagtg atcacgaatc tgctccgcca gattttttcc ggtggcaatc cctagtttgg
    13261 cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct
    13321 gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac
    13381 caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc
    13441 cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta
    13501 tgtttcatgc tatgataaac gagaatgatt atcaaattca tttaaatgga ttgtggtgat
    13561 ttctctgata gaccaggatt atgactacga aaagattgat gacttgaatg ttatggttat
    13621 gtttaatgtt agcacattta cataactttc agcttccata cacaacatag cagaaatgta
    13681 tgacagatca ctatttttga agcctgtcac aggacgtcat tatagtgtgt gtcagatctc
    13741 gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga
    13801 gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgc gattctcgca
    13861 ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt
    13921 atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa
    13981 ggcttctccg aaatgttcga aaaactgctc agatttgcca acgaagggac taacttcgtc
    14041 tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc
    14101 gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc
    14161 cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac
    14221 gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctc
    14281 ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg
    14341 atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg
    14401 gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat
    14461 gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg
    14521 ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc
    14581 ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt
    14641 ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg
    14701 atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt
    14761 tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt
    14821 gctgaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt
    14881 atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt
    14941 ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg
    15001 gtgctgtaaa accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt
    15061 aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct
    15121 tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact
    15181 tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa
    15241 tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt
    15301 cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca
    15361 gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct
    15421 caagaatgac cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg
    15481 caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt
    15541 tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac
    15601 gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc
    15661 cgtcatattt cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg
    15721 gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata
    15781 gcacctgttt agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat
    15841 gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg
    15901 tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat
    15961 tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg
    16021 tttccggccc gctcagcact aacagcagtt cactacccgg caattgatat aacttttcgt
    16081 tttcctgcat caacggcagc agcgtacggt aaattgatcg gatacagtga acgcgcatca
    16141 ttaagccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaactctt
    16201 gcccgcctct tgctccggcg cttgttccaa cgcacggaag ttgggcagga gcgttaacgg
    16261 atcggtcagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    16321 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcaagaataa atgccagcga
    16381 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    16441 aacagccctg gtgagattta aaaacggata gcgaagctta ccgaccccga gggtaaagat
    16501 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    16561 cagaagtaga agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    16621 gcccaacgac ggcgcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    16681 catgcgagtg agatagtaga aaagcatgtt gtaaataagc acagcggtga aaaggcttag
    16741 caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    16801 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    16861 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    16921 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctaccgag
    16981 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    17041 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    17101 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    17161 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    17221 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    17281 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca gggggccagc ctgttgccga
    17341 tttttcatcc aatgatgctt tcaccaggcg gcactaaccc ggacgtagac tgtattacaa
    17401 aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg
    17461 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    17521 gctatagccc cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct
    17581 aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat
    17641 agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat
    17701 gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa
    17761 ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt
    17821 tataaaatga actactaata gacccacata cattcaggga attgttatgt tcaaggagag
    17881 gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg
    17941 ggtacgcaag gagggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg
    18001 actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac gtccagaagg
    18061 tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact
    18121 ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg
    18181 gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac
    18241 gcaataaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta
    18301 accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc
    18361 gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct
    18421 tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac gctcctggag
    18481 cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca gacattgagc
    18541 agggaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg
    18601 atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc gtttatcttt
    18661 ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc tttgttgata
    18721 cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc tggagactgc
    18781 cccgccccgg ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc
    18841 tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg
    18901 gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc
    18961 gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc
    19021 tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg
    19081 ttacgggtat cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc
    19141 ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca
    19201 ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag
    19261 atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc
    19321 aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt
    19381 ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg
    19441 gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg
    19501 tgggtgattt ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca
    19561 tcggtacggc ggataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg
    19621 atctgatcgt caaaaacaac agaaccttcc tgcgggttag caaaacgtac aacacacggt
    19681 tcatcatcag catgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca
    19741 cctttcgcca tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta
    19801 taagcagtgc cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg
    19861 gtctggtagt acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg
    19921 gcttcgatag cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac
    19981 tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg
    20041 tgcagatagc ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac
    20101 gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc
    20161 actcccgcct gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt
    20221 gacaaattgc gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa
    20281 caatttcttt agaaaatgcg ttgactcatt ttgaactctc cctataatgc ggctccacac
    20341 agcgggggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    20401 cgatcccgtc atcacccacc aacatacttt atgtagtctc cgccgtgtag caagaaattg
    20461 agaagtgggt gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt
    20521 tcgatcccgt catcacccac caatttctcg ccagctaaat ttcttgtaaa aatgtgaagt
    20581 accgaagtgg gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg
    20641 gttcgatccc gtcatcaccc accacttcgg gtcgttagct cagttggtag agcagttgac
    20701 ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc
    20761 taaaggcgct tttttgctat ctgcgatact caaagattcg aacctgcagc aggtttgagt
    20821 tgagcgcagc gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga gcgaagcgag
    20881 tcatcctgca cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctatctgc
    20941 gatttgtaag attgattttt cttctcaagt tccttgttct tctgccgata ttacctcgta
    21001 cagtgacgag gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac
    21061 acagagtagt aacagggcat cacaaggtag cgatgttgct tcaaaaatcg cgcggataag
    21121 tcagcaaatc atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc
    21181 tgaagacaaa caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca
    21241 actggcgcag ttgcagagac agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc
    21301 tctgaatgta tcccttctaa atcctgttga aaatacgact catatcgata tctatatctg
    21361 atacaacgga gccccttccc gaccagccat tagcgcattg ccctggtctc gtaccacgtc
    21421 ccataacgct tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg
    21481 gcgttcaaca accggcgtaa tgcgctttac caccagtggg ctaccttcag gcaatggtag
    21541 cgccagtgcg ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc
    21601 cggatgtcca atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc
    21661 aatcagcggt cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc
    21721 ctgccagggt acataatctt ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa
    21781 aggctcggaa agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat
    21841 gcaaaaatcg acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg
    21901 caggacaaac tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag
    21961 atgcgcggaa atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat
    22021 acgcccggta tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc
    22081 caactgctgc cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac
    22141 accagtatga ttttccagct ccttcacact gtgacttact gccgactggc tcaagccgat
    22201 acgctctcct gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg
    22261 ttttaaagaa taattcatct gttttcttca tgaatggatg caataaatca attttatttc
    22321 tcaaactgga agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc
    22381 gtatcctcga tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc
    22441 cggccagagg cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc
    22501 tgttctttat gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact
    22561 ggcgactgca tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac
    22621 tgtttgcctg gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact
    22681 tgtgcattct cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta
    22741 acgtcgcggc ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac
    22801 cattgctagt tggtctggtg atgaatgttc acggtgcagg gggcagcctt gagcaggtcg
    22861 gtaaaattat gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga
    22921 ttggtgactg ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc
    22981 tgttggtggt ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg
    23041 gctggggatc attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg
    23101 tagttaacgt ctttatggca cgccgactgg gcttcaataa ggcagatgaa attactatcg
    23161 tcttttgtgg ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc
    23221 ccacatcggt gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga
    23281 tggtctgtgc ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcgcagc
    23341 aggaaagcag cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca
    23401 gcccctcggt tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga
    23461 actcatcttt gcgggcaaac cacagcagat actcctccgg caggtcgatt aagcgacgcc
    23521 ctttgtattt gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca
    23581 tctcagctac ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc
    23641 tgcgccttcg ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc
    23701 gacacgctgc ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc
    23761 gacatctggc ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc
    23821 tcttccgcgt tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg
    23881 atgacattgc ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg
    23941 ccaacatcag gcaccttttg cagctcttca atcgaagcgg cttccagcac ttccagcgtg
    24001 ccgaaatatg ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca
    24061 tagaggaagc gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt
    24121 gccgattttg gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga
    24181 tctgccggag tgtggacata ttctttttca accagctgat cgatgatttt gtcgcccatt
    24241 ccgtcaacat ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca
    24301 ccgcaaatca ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca
    24361 gaaccacata ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca
    24421 gaaagcacga cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta
    24481 tcgccaatgc gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc
    24541 accagcacgc cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta
    24601 cgcccaacct gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta
    24661 aacgctaccg cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt
    24721 gagttgacct taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc
    24781 actttgtgat agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg
    24841 accggcaacc cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg
    24901 ccaccttcca gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata
    24961 cgcggatcaa gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc
    25021 ccgcccgtgc gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact
    25081 tcaccacgca cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatggcg
    25141 cgaatagtac gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg
    25201 gcactgacta aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc
    25261 tcacagcacc aggtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa
    25321 gcaagaaagc tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt
    25381 atctggctga aagccgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta
    25441 atcagttctg gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca
    25501 gcgtcgggaa tttccggcgc atccatcaca tgataaagat attcatgatg gcgaagcgtc
    25561 gttcgcagtt ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa
    25621 cccccgacaa gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc
    25681 gtctttgact tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg
    25741 gcgctgatcg tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag
    25801 catcagcttg aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac
    25861 acccggagta gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc
    25921 caggctgaat aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat
    25981 atcgccaaaa atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc
    26041 gctaccgtga tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc
    26101 cataactgga gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg
    26161 ctggaaagcc tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac
    26221 ctgtggcgaa actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg
    26281 catcggctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg
    26341 gatcggttga cgcggctgcg cagacgcata aggcggttgg tactggtgtt gcggcgacgg
    26401 acgagcagcc tcatgctcct gagcgttagc cggggcatga ttcacgcggt gaacacgaac
    26461 ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga
    26521 cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca
    26581 gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg
    26641 caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt
    26701 actcactaag agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca
    26761 caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg
    26821 cgcaattacc cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg agctcatcct
    26881 gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg
    26941 ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa
    27001 tattttgttg atggggggcg cattctggtg gctctttaca cagctcgatg tctggatccc
    27061 gactctcatg agttacgttc cggactggct acaatggctg agttatctgt tgtggcctct
    27121 ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat
    27181 tgccgctccg tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac
    27241 accgccagat accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga
    27301 atggcaaaaa tttgcctggt atctgccgcg cgcaattgta ttactaattc tttacttcat
    27361 ccctggtatt gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt
    27421 agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg
    27481 caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt
    27541 tacgatgatc ccgctgctta atctgttcat catgcccgtt gccgtttgtg gcgcgacggc
    27601 gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta
    27661 ttttgtaaac cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt ccatcttgca
    27721 tgtcattatt tcccttctgt atatagatat gctaaatcct tacttccgca tattctctga
    27781 gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga
    27841 tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg
    27901 gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc
    27961 gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg
    28021 aactggttga accgaccagc ggtaataccg ggattgcact ggcctatgta gctgccgctc
    28081 gcggttacaa actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc
    28141 tgaaagcgtt aggtgcaaac ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa
    28201 tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat
    28261 tcagcaatcc ggcaaaccct gaaattcacg aaaaaaccac cggtccggag atctgggaag
    28321 ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg
    28381 gcgtcagccg ctacattaaa ggcaccaaag gcaagaccga tcttatctct gtcgccgttg
    28441 agccaaccga ttctccagtt atcgcccagg cgctggcagg tgaagagatt aaacctggcc
    28501 cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taacctcgat ctcaagctgg
    28561 tcgataaagt cattggcatc accaatgaag aagccatttc taccgcccgt cgtctaatgg
    28621 aagaagaagg tattcttgca ggtatctctt ctggagcagc tgttgccgcg gcgttgaaac
    28681 tacaagaaga tgaaagcttt accaataaga atattgtggt tattctacca tcatcgggtg
    28741 agcgttattt aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt
    28801 aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg
    28861 cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga
    28921 ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt
    28981 atgatttggt tcaattcttc ctttaacggc ataatgttta atgacgtacg aaacgtcagc
    29041 ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa
    29101 ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac
    29161 taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa
    29221 cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga
    29281 aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac
    29341 tctgggcctg actcaaggta ccgttgtgac tatctccgca gaaggcgaag acgagcagaa
    29401 agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta
    29461 aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc
    29521 gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt
    29581 tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca
    29641 gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc
    29701 atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc
    29761 ctgattaaag ataaacacat gacagctgac gcagctgctc atgaagttat cgaaggtcag
    29821 gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt
    29881 gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc
    29941 attcaggatg aagtcattct ggttgccgct gacctgacgc cgtccgaaac cgcacagctg
    30001 aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct
    30061 atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag
    30121 gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca
    30181 accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca
    30241 gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca ggtagaagta
    30301 tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc
    30361 gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa
    30421 gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt
    30481 cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag
    30541 aacccgttcc tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc
    30601 gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg
    30661 atgatcatct ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag
    30721 gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa
    30781 acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc
    30841 ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca
    30901 cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct
    30961 tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct
    31021 acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg
    31081 cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag
    31141 gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa
    31201 aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg
    31261 tttgttcgat aaactgaaat ctctggtttc cgacgacaag aaggataccg gaactattga
    31321 gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt
    31381 tgcggaaaaa atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc
    31441 gcctgtagac ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc
    31501 tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga
    31561 aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga
    31621 atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg ttgttatctc
    31681 caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtaaccg tgggtgaaac
    31741 cccggttatc cgcatcaaga agtaattctt gccgcagtga aaaatggcgc cca
//
LOCUS       DAJTYZ010000037        31085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000037 DAJTYZ010000000
VERSION     DAJTYZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.037"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..278
                     /locus_tag="OO596_001642"
     CDS             <1..278
                     /locus_tag="OO596_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6717517.1"
                     /translation="SVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTR
                     FGKDMPGAMAEYGREYMKRKTKHGLRSNVTLTEDSEDWMPSCAIGHK"
     gene            582..827
                     /locus_tag="OO596_001643"
     CDS             582..827
                     /locus_tag="OO596_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717518.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            824..1246
                     /locus_tag="OO596_001644"
     CDS             824..1246
                     /locus_tag="OO596_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6717519.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATIWLSVLAFGRQADALAKHQKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            1906..3773
                     /locus_tag="OO596_001645"
                     /pseudo
     CDS             1906..3773
                     /locus_tag="OO596_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
     gene            4032..4313
                     /locus_tag="OO596_001646"
     CDS             4032..4313
                     /locus_tag="OO596_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717520.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQAMQPDSVTAENYARVVDEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            4953..5273
                     /gene="clpS"
                     /locus_tag="OO596_001647"
     CDS             4953..5273
                     /gene="clpS"
                     /locus_tag="OO596_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6717521.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            5304..7580
                     /gene="clpA"
                     /locus_tag="OO596_001648"
     CDS             5304..7580
                     /gene="clpA"
                     /locus_tag="OO596_001648"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6717522.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(7986..8073)
                     /locus_tag="OO596_001649"
     tRNA            complement(7986..8073)
                     /locus_tag="OO596_001649"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8037..8039),aa:Ser,seq:gga)
     gene            complement(8327..8545)
                     /gene="infA"
                     /locus_tag="OO596_001650"
     CDS             complement(8327..8545)
                     /gene="infA"
                     /locus_tag="OO596_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6717523.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(8830..9534)
                     /gene="aat"
                     /locus_tag="OO596_001651"
     CDS             complement(8830..9534)
                     /gene="aat"
                     /locus_tag="OO596_001651"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6717524.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(9576..11297)
                     /gene="cydC"
                     /locus_tag="OO596_001652"
     CDS             complement(9576..11297)
                     /gene="cydC"
                     /locus_tag="OO596_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6717525.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(11298..13064)
                     /gene="cydD"
                     /locus_tag="OO596_001653"
     CDS             complement(11298..13064)
                     /gene="cydD"
                     /locus_tag="OO596_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6717526.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(13187..14152)
                     /gene="trxB"
                     /locus_tag="OO596_001654"
     CDS             complement(13187..14152)
                     /gene="trxB"
                     /locus_tag="OO596_001654"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6717527.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            14697..15191
                     /gene="lrp"
                     /locus_tag="OO596_001655"
     CDS             14697..15191
                     /gene="lrp"
                     /locus_tag="OO596_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6717528.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            15326..19393
                     /gene="ftsK"
                     /locus_tag="OO596_001656"
     CDS             15326..19393
                     /gene="ftsK"
                     /locus_tag="OO596_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6717529.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            19548..20159
                     /gene="lolA"
                     /locus_tag="OO596_001657"
     CDS             19548..20159
                     /gene="lolA"
                     /locus_tag="OO596_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6717530.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            20170..21513
                     /gene="rarA"
                     /locus_tag="OO596_001658"
                     /gene_synonym="mgsA"
     CDS             20170..21513
                     /gene="rarA"
                     /locus_tag="OO596_001658"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6717531.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            21604..22896
                     /gene="serS"
                     /locus_tag="OO596_001659"
     CDS             21604..22896
                     /gene="serS"
                     /locus_tag="OO596_001659"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6717532.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            23135..25579
                     /gene="dmsA"
                     /locus_tag="OO596_001660"
     CDS             23135..25579
                     /gene="dmsA"
                     /locus_tag="OO596_001660"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCR6717533.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            25590..26207
                     /gene="dmsB"
                     /locus_tag="OO596_001661"
     CDS             25590..26207
                     /gene="dmsB"
                     /locus_tag="OO596_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6717534.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            26209..27072
                     /gene="dmsC"
                     /locus_tag="OO596_001662"
     CDS             26209..27072
                     /gene="dmsC"
                     /locus_tag="OO596_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR6717535.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(27108..27734)
                     /locus_tag="OO596_001663"
     CDS             complement(27108..27734)
                     /locus_tag="OO596_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6717536.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            28049..29197
                     /locus_tag="OO596_001664"
     CDS             28049..29197
                     /locus_tag="OO596_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6717537.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            29407..30837
                     /locus_tag="OO596_001665"
                     /pseudo
     CDS             29407..30837
                     /locus_tag="OO596_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(30838..>31085)
                     /locus_tag="OO596_001666"
     CDS             complement(30838..>31085)
                     /locus_tag="OO596_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6717538.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         7870 a         7502 c         8287 g         7426 t
ORIGIN      
        1 cgtcggtaat gtgtgatggt ctgttagtgg gtaatgccga aattattccg ttcagtccgc
       61 gcaggtatct ttatcacgcc tatctggcat atatgagggc acacggattc ggtaaacctg
      121 taacactgac gcgcttcggt aaagatatgc cgggggcaat ggcggaatat ggcagggagt
      181 atatgaaacg gaaaacgaag cacggtttgc gttcaaatgt gaccctgaca gaggattcag
      241 aagactggat gccatcatgt gcaataggtc acaaatgacg atggaaaaaa ttaaacttat
      301 ggaataactg ttcaccactg ttcaccctgc aataaatgtc ttttatatca gtatattata
      361 gggtgaacag ttatttatga actgttcacc aaactattca ctgttcacct ttttgattct
      421 ttattgagct ttaagggtga acagtggtga acagttggtg aatagttttt gtgaaactgt
      481 taacccatta acatcatgaa taaaaaggga aaattgcaaa aggtgaacag gtgaagggtt
      541 aaaatgcaaa aattttattt tattgctgtg aggtaaagcc tgtgacaacg aagcacgcca
      601 aaaaaccaca atctcacgcc cttgatttga cagaacactg gctgagggtg gcgataaaaa
      661 tcatcgaccg caacgccggg gaaggatacg cgaaagcaca tcccgaactg ataagcgcat
      721 tcatgaccac gacggcggca aactttgcca cgctgacaga acgggagatt gccgaagcgg
      781 aacaggtgac aaccatcaac gttaaaaccg gagagcagac agcatgacag cacagatagc
      841 ggcttacggg cggctggtgg ctgacccgca gttaaagacc accagcaagg gtacgcaaat
      901 gacgatggcg agtatggcgg ttcccctgcc gtgcagccag gcagatgacg gaacggcgac
      961 gatatggtta tccgtcctgg cgtttggcag acaggctgac gcactggcaa agcaccagaa
     1021 aggcgagctg gtgagcgtgg cgggtaacat gcaggtaagc cagtggacag gccagaacgg
     1081 agaaacgcgg cagggctggc aggttatcgc agacagtgta atcagtgcca gaacggcgcg
     1141 accgggcggt aaaaaaggcc aacaggggca ggccactgat gcactgaaca gggccaaaca
     1201 acaggcgggg aatgatgatc cgtacgggga taacataccg ttttaagcaa cgagtgacag
     1261 aagccggagc aatccggctt ttttgtaggt actcctggtg ggggtggcct gtccacgggg
     1321 cgggcggttc gcggaaaaag gcgtattttt tgatttttat gtcaccatca ccaccagttt
     1381 aagttattga tatatggcaa aataaaaatt tttagtgtcg aattaggttg ttttttgttc
     1441 gacgccggaa cgttcccgaa agcatttaca aaaaacaggc gcaaaaaaaa gcgccccgat
     1501 ggtgccaccg gaggcgcttt gtcagcgaca aaggatactg ccactaccga aatgatgagt
     1561 cttaattgtg ctggcgtggc aataattagt caattctttt gctggtatga caaaaatcag
     1621 aaaacacata atctgatttt tagatagaaa atgatttatt taccagtttt atcgatcaat
     1681 aatgatgccc gttaatcata acggaggcag acttatgcca gagaacaaca gcagaaaacc
     1741 ggataaaagt gcaacagtgc acatagacgc cggaactatg gagaagctcg aacgctatca
     1801 gcagttcatt aaaagtcatc accctggaat gccagtaccc acgaaagggc agattgcgag
     1861 aagcgcggtt gaatactggt acagggcaac gttaggagcc tggctatgaa aacatggttt
     1921 tccattaagg ctatggctga tgttgtacat gtacgcattt atgacgagat cggcgggtac
     1981 ggtgtaaaag catcggcact tactgacgag atcaacgcgt gcggtaatgt gcctgaaatc
     2041 catcttcgca tccattcacc tggtggcgac atctttgaag ggctggctat ctataacgcc
     2101 ctgaaaaatc atccggcaaa gaaaattgta cacattgaag gcatggcggc ttctatggcc
     2161 tcgtttattg ccatgtgtgg cgatcacatc gttatgcctg aaaacgcgat gatgatgata
     2221 catgcccccc gtggtgttac tgccggagtg tcgggcgacg ttcgccgctt tgctgacctg
     2281 atggacaagc tgggcgacac gatggcggaa acctacgccg gaagaacggg caggagcaaa
     2341 caggaaatca ccgccatgat ggaggcggaa acctggatgg atggcaatga gtgtaaggct
     2401 aatggcttcg cagatgaggt tatacccgcg attacagcaa tggccagaat tgaatcaaaa
     2461 cgaatcggag atttttcgaa tatgccggaa aatattaaaa gcatgatcag ccagaaaact
     2521 ggcagcggag aacaggaacg actcaacggg atacgtgaat tgtttggcac gttcaatgga
     2581 agatataacg acctggctat aagttgtctt gctgattcag gctgtagcgt tgaggatgca
     2641 cgcgaacgcc ttttactcac tatgggtaaa gaatcgacac cgacaaacaa aaccaccccc
     2701 gcaaatcttt actacgcgta cacggataac ggcaacataa ccggcgatgc aatgcgtcag
     2761 gggcttaatg cgcgtctggg acatgaacag gcagaacgcg gcaaccctta cgccatgatg
     2821 agccttttcg atatggcaca ggcatcatta acccatcgcg gcataagcac aggcagctac
     2881 agcacacgct cgcagatagt aaacgcggca ttcacccaca gcagcagcga ttttacttat
     2941 atccttgctg gtggtgctga aaaatccgtg aacacagcgg cgaaacgttc cgccagtgga
     3001 cgaaaaaagg ttcattgtca aacttccatg aggcacgtcg tgttggcctg aatggcttct
     3061 ctacattaaa caaagtgccg gaaggcgcgg aatataaata catcaccacc agtgaccgtg
     3121 gcgaaccaat cgcgctggca acttacggaa atattttcag cattacccga caggcgatta
     3181 ttaacgatga cctgaatcag ttaacagtta tcccacaggc aatggggcga gcagccgcca
     3241 gaacggtggg taatctggtt catctggtgc tgacaggaaa cacaaagctt tctgacggta
     3301 taccgctgtt cagcaaaaaa cacggaaatg atattgataa cggagtcagc actacagggc
     3361 ttggcattgc acgccgtgca atgcgcacac agagggatga aaacggtgag gtgctgaata
     3421 ttgtgccaaa attccttctt gttcctgcgg ctctggaaga cagagcgttg caaatggtta
     3481 actcaaccac gcctttcggt gccgatacaa acagtggttc atataacccg taccacaaac
     3541 aactggatat tattgtcgat ccccgccttg atgaagtcag tgataagcaa tggtacatgc
     3601 tttcagcaca gggaacggac acaattgagg tggcttatct tgacggtaat gacgagcctt
     3661 accttgagca gcaggagggc tttattgttg acggtgtggc ctggaaggtc cgcattgatg
     3721 ctggcgttgc tgctctggat tatcgcggaa tggtcagagc gatttcttca tgagaacaag
     3781 gcggcagcag ccgccttttt tgcgggtcct cctggtgggg ggggcctgtc cacggggcgg
     3841 gtcggcgcgg aaaaaagcgc atttttgtgg ttttatcgtc atcatcatca tgtgtgtaac
     3901 ctgttgtttt taatgtagtt gatgcaaaaa agatgatgat tgtggttaat ttttgttcga
     3961 catcttttag cgtgacagat tctttacaaa aaatctgagc ttgttttctt aaccagcgcg
     4021 atgggggcac aatgacagaa gccgaaatac tgggattaat ccgccgcgtc gccggaatca
     4081 gccagcaggt tgacgaacag gccatgcaac cggacagcgt gaccgctgaa aattatgcgc
     4141 gtgtggtgga tgaggtaatg cgccgtgacg gtattgagct taacggcgtg gatatgcgca
     4201 acatacgaac cagagttctt gagttgctgg cataccgtcg ccgttctcaa caacggaggg
     4261 agagcgcgaa aaatacttac cagtggagga agccggagca cttacggcgg taactggttg
     4321 atattaccga aagtgcaaaa atgcacttag caactggtgg cgagttgcag atctgcaact
     4381 cgactatgaa actacggaaa ctacccgtag tttgggtagt aagagtaaca cccagatttt
     4441 ggggcttact cgcgataccc aaataaaggg tatcggtgga agaaatatcg tttctcatat
     4501 gtgagtaccg agggcggaat tccgccttcg gttacttatt gtgcgcatgc acagggaggg
     4561 gcgggtcaaa tctctgtacc ctgacgtctt ccggactgcc agccccatcg attttttata
     4621 cccgcgaaaa atgaaaaacg cttcacgctg gtgggcctga tgccgataac tggtttcatg
     4681 actgatagcc ggaaaatgat aacgattaaa gcgtgttgat ctttgttcgt ggttgttcga
     4741 tattgttcgt ggaacggtgt agttaatggt gtagttaatt ttactgtttt tgacgagctt
     4801 attgtttgca actcaatgaa aaataaaggc taataagcca aatgctataa atgatagtta
     4861 tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca gggcgttctg
     4921 ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac tggctggact
     4981 ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct atgtataaag
     5041 tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg ttacaaaaat
     5101 tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac taccagggga
     5161 aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg atggtgaaca
     5221 agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc tgaatgcagg
     5281 cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt ttaaatatgg
     5341 ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac ttgttactgg
     5401 cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg gatttggttg
     5461 cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg cctgccagtg
     5521 aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa cgtgcggtct
     5581 tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg gtcgctatct
     5641 ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc agccgtctcg
     5701 atgtggtgaa ttttatctct catggcacgc gtaaagacga gccgacacag tcttctgatc
     5761 ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg gagaatttca
     5821 cgacgaacct gaatcagctt gcgcgcgtgg gcggaatcga cccactgatt ggtcgtgaga
     5881 aggagctgga gcgtgctatt caggttctct gccgtcgccg taaaaacaac ccgctgctgg
     5941 tgggggaatc tggtgtcggt aaaaccgcga ttgcggaagg tcttgcctgg cgaattgttc
     6001 agggcgatgt gccggaagtg atggctgact gtacgattta ctctctcgat atcggttctc
     6061 tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg ttgctcaagc
     6121 agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc attatcggtg
     6181 cgggggcagc gtctggtggt caggttgatg cggctaacct aatcaaaccg ttgctctcca
     6241 gcggtaaaat tcgtgtaatt ggttcgacaa cctatcagga gttcagcaac attttcgaga
     6301 aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg tcaatcgaag
     6361 aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac gacgtgcgtt
     6421 ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatc aatgatcgtc
     6481 atctgccgga taaagcgatt gatgttatcg acgaagcggg cgctcgcgca cgcctgatgc
     6541 cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg gtggcccgta
     6601 ttgcacgcat tccagagaag agtgtttctc agagtgaccg cgataccctg aaaaacctcg
     6661 gcgatcgcct gaaaatgctg gtcttcggtc aggataaagc cattgaggcg ctgactgaag
     6721 ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt tcgttcctgt
     6781 ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg aaagcgttgg
     6841 gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc gtcagccgtc
     6901 tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg accgatgcgg
     6961 taatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg catccggacg
     7021 tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac aacggacgca
     7081 aagcggactt ccgtaacgtg gtgctggtga tgaccaccaa cgccggggta cgggaaactg
     7141 agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg gaagagatca
     7201 agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg tttgatcatc
     7261 tgtcaaccga cgtgatccat caggtggtgg ataaattcat cgtcgagttg caggttcagc
     7321 tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg ctggccgaga
     7381 aaggttacga ccgggcaatg ggcgctcgtc cgatggcgcg tgtcatccag gacaacctga
     7441 aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt caggtcaccg
     7501 tcgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca caaaagcaca
     7561 aggcggaagc agcgcattaa tctaattgtt aggtaggttg gtgaagtccg taatctcgaa
     7621 agaggttacg gactttttgt ttatggggtg gaacggtgaa aaccctattt ttggaggtga
     7681 aggtaatttg ttgataatta gtgctgctgg aaaaggtaag gataaaaaag ggtgacagca
     7741 ggaaaatgag atggttttgc tttattaaca acggactaaa cgtgtagtat ttgagttcac
     7801 tgccgtacag gcagcttaga aatcgacggg gtgcggtaaa acctttgcga acgcgttcac
     7861 tgccgtacag gcagcttaga aattcacagg taacatactc cacccgccca ccatgttcac
     7921 tgccgtacag aaagataaaa tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt
     7981 aaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccgtatac acactttcca
     8041 ggcgtgctcc ttcagccact cggacacctc accaaattgt tttgctacca aacctcatgg
     8101 gtggcaacgg ggcgctacta tagggagttg gagtaaaacg gtcaagaaga attttaatga
     8161 taattattgt ttgctcatac tgtaaacaag ttgtgcagta tatctacatc gagacaagtt
     8221 acggacttat acttccaaag tacttcatac atatcacaaa ataaaaaggc cggttaaacc
     8281 gaccttttac tcgttctttc tcttcgccca tcaggcggta aaacaatcag cgactacgga
     8341 agacaatgcg gcctttgctc aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg
     8401 tcaggatgcg gatgtagttt ttgcgcattt taccggagat gtgtgcagta accacgtgac
     8461 cgttttctaa ctctacgcgg aacatggtat taggcaacgt ttcaagaacg gtaccttgca
     8521 tttcaatatt gtcttctttg gccatctaat cctctggggt atcactaccg taatttgaac
     8581 cggcaagata atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa
     8641 acaggttcgg cacattactc cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat
     8701 aagggagcgg tgagataaac gatgggcgtt acctgacgcg aaaaattcct tatcggcagc
     8761 ggggtaatga gcataaccaa ctctgcgacc gcaattataa cactctgggg agaaatgtgc
     8821 cgaaaacatt catttttgtg gtgaaaacaa gcatcgtggc acccagaaat tattcggcaa
     8881 tcgtccgagg cgcatttgat tgagataatt cagataatcc cgacggggaa tttcgcaggc
     8941 accaagcgat gctgtgtgat cgttaaggac ctggcagtcg ataagcttac cgccatgact
     9001 gataaattcc tcacagaata ccagaagcgc cgttttagac gcattttcca tccggctgaa
     9061 catggactcg ccacaaaata gcgttccctg ggccacgccg tacataccgc cgacaagctc
     9121 atcttcatgc cagacttcaa tggagtgggc atgaccgagt tcgtgaaggc gatggtaggc
     9181 ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac aaccttcaat
     9241 gacctgacca aaagcgtaat tcatcgtgac acgatagggc gagcgtttat gaaatcgctt
     9301 catactacgg ctgatatgca gtgattcagg ccaaagcacc gcgcgaggat cgggcgacca
     9361 ccagaggatg ggggcgcctg gagaaaacca cggaaaaata ccacgctggt aagccattaa
     9421 caggcgcgca gggctaagat cgccccccag agccagcagg ccgttaggct cacgtaatgc
     9481 gccttccggg gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc
     9541 aaaactccac gcaagtcgga tcgttcaata atagcttaca aaccctgctt aaactggtaa
     9601 taacgcccct gtctggcaag cagttctgcg tgagtacctt gctcaataat ttgcccgttg
     9661 tccatcacta ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt gaccattaac
     9721 accgttttct cacgcatcat ttctgcaagc aattcaagga tctggctttc ggttgtggca
     9781 tctaagcctt cggtaggttc atccagcaac accagtggcg catcatgtaa cagcgcacgg
     9841 gcgatagcca gacggcgcag ttcaccaccg gagagctggc gtccgccttc acctaaccaa
     9901 ctgttgagac ctgcgtcctc gagcagcttc tccagaccaa cgcgacgcaa gatctccgac
     9961 agagcctcat cactactgcc aggcgaggcg agtaacagat tatcacgcag cgtggcgcta
    10021 aacagatgca ctcgctgagg aacaacgctg atggtctgtc gtagagccgc ttcattcagg
    10081 ctggctatgg ggctatcgtt aagcaaaatc tcgccctgtt gcgggtccca agcgcgggtc
    10141 agcagttgta acagtgttga tttgccgcat ccggttcgcc cgagaatcgc aatatgttcc
    10201 ccggcgttta cctgaagaga aatcccttta agtgcctgtt gggattgctc cggataagtg
    10261 aactgaacat cccgtaatgt cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg
    10321 aaggtgactt ccggtttttg atccgttaaa tcagtgatcc gaacggcaga ggcaatgact
    10381 tgccccagat gctgaaatgc acccgttact ggtgccagtg cttcaaacgc ggctaacgcg
    10441 cagaagacaa acagggcaat taacgcgccg ggttgagcat tgccgccaac gccgccagac
    10501 gccatccaca gcatcaggat caccgctaac gcgccaatga gcagcattat cgcttgcgac
    10561 aatgcggtca gttcagattg acggcgttgc gcttccagcc attgaatttc tgtattctct
    10621 agttgcgtgc gataacgatc gctggcacca aaaatggtca gctcagcttg cccttgcagc
    10681 caggccgtca gttgttggcg atactgtccg cgaagatgag tcagattttg cccggtgctt
    10741 tttcccgcac gataaaacag cggtggcatc aggaaaagcg tcagtaacat aatgccgccc
    10801 agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga ctgtcaccac cataatcacc
    10861 acaaaagcgc ccaccagcgg cgagataacg cgcaggtaaa gatgatcgag cgtatcaaca
    10921 tccgccacca cgcgattgag caattcgccc tgacgatagc gcgccagtcc ggcaggggag
    10981 aggggcaaca atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg gaaagtcgcg
    11041 tcgtgactta ccagacgttc aaaataacgc ccggcagtac gggtgattgc ggcaccacgc
    11101 acgcccgcag cgggtagcat atagttgaag ctgtacagtc cggcaccccc cgcaaccgct
    11161 gaggccgaga ggaaccagcc ggaaagtgtc aacagaccga tactggcgag cagcgtcaca
    11221 attgccagca caataccaag acttaacatc catttatgac gtttatacag tgccagatag
    11281 ggtagcaaag cgcgcattta aatctcctcc tgacgatggg ccagtaatgt ggcgaatggg
    11341 ccaccagcca cacttaattc cgcgtaacgt ccttgctcaa taatctgacc atcctgcatt
    11401 acccaaatga catcccagtc agcaagatct tctaactggt gggtgaccat taacgttgtc
    11461 tggcgcagag aggcggcatt cagcgcctcc attacgcgct gttcactgtg agcatcaagg
    11521 ctggcagcgg gttcatccaa cagtaatagc gaacagggat ttagtaacgc acgggccacc
    11581 gccacgcgct gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac aggcgtatca
    11641 acgccttgtg gcaggagcgg tagaaactcg ctgacccagg cgttatccag cgctgcttgt
    11701 aattcatgtt cgctggcatc aggtcgcgcc agtagtacgt tatcccgtaa tgttgctgcc
    11761 ggtaattgtg gattttgccc aacccaggag agatgtttac gccatgattc tggtgataaa
    11821 tcgcgtaatt ctatcccgtt gattcgtagc gatccctgat atgagagaaa accagaaagc
    11881 gcgttcaaca gtgaactttt acctgaaccg ctgcgaccaa ccaacacggc acgttggcct
    11941 gctggcaaag taaagttcag cggtccggcc agcgttttac cttccggcga cgtgataaac
    12001 agatcctcgg cctcaatggt caccggatcg gtcgatgcta attccacctc gccacgctgc
    12061 ggatgggcga gcggggtttc cataaacgtt ttcagactgt cagctgcacc aacagcctgg
    12121 gctttagcat gataaaacgt accgagatcg cgtaatggct ggaaaaactc tggcgcaagg
    12181 atcagggcca gaaaacccgc agccagcgtc acgccagtat cgtagtgacc aaaatccagc
    12241 tcgccgagat aggaaaaacc aaagtagacc gccaccagag caattgacag cgaggtaaaa
    12301 aattcgagaa tgccggagga taaaaatgcc agccgtagca cttccattgt ccgttggcgg
    12361 aaatcttccg aagcagaacg aatactttca atttcagctt caccacgacc aaaaatacgc
    12421 aatgtttcca tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga
    12481 aagttacgtc ggttagcatc ggcagccccc attccaacca gcgccataaa caacggaatt
    12541 aacggtgcag tgcccagcag aatgagcgcc gcagcccagt ttgaggggaa gattgccacc
    12601 acaatcagca acggcaccga cactgccagc gccatttgcg gcaggtagcg tgcatagtaa
    12661 tcatgcatat cgtcaatttg ctcgagtacc agcgtcgccc agctccccgc aggtttaccc
    12721 tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag
    12781 cggatatgct gcccggcgtg ataacccacc cgttcgcgta accagaccgc ccatgcgcgc
    12841 agtacaaagg tcagaaccag taacgtaaag ggaagcagca gggcttcacg gggaatattc
    12901 tccataatca tatgttgcag aattcgcgcc atgaaccagg cctgggcaat gatcaatatg
    12961 ccgcttacaa agcccagcag acgagaaata ttcagccaac gttgggagat gacgctttgc
    13021 tgttttaacc agcgggttaa ctctttttga cgagatttat tcattgcacg cttagcaggt
    13081 gagttatcag aattatttgc agagcaatgt tacaacgggg aaaaaataaa ggcgacccat
    13141 agtcgcaagg tgtcgccttc tttacttttg ttactgattt gtaaaattat tttgcgtcag
    13201 ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat gcagccagta ccggccgaag
    13261 taatggcctg gcgataaatg tgatccatca cgtcgcctgc ggcaaagacg ccaggaatgc
    13321 tggtctgggt ggcattacca tgaatacccg actgtacttt gatgtagccg ttttccagtt
    13381 ccagctgccc ttcgaaaatc gcagtattcg ggctgtgacc gatagcaaca aacagaccgg
    13441 caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga acgccagtga
    13501 cacccatttg atcgccggtc acttcttcca gcgtacggtt ggtgtgcaga atgatgttgc
    13561 cgttctccac tttatccatc agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac
    13621 ggcggtgaat cagatgcact tccgaagcga tgttagacag atacagcgcc tcttcaaccg
    13681 cggtattgcc gccgccgatg accgcaactt tctggttgcg atagaagaaa ccgtcgcagg
    13741 ttgcacaagc agaaacccca cggcctttaa aggcttcttc agagggcagg ccgagatagc
    13801 gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt gtattcgccg ttatcgccat
    13861 tcagacggaa cggacggttt tgcagatcca ccttgttgat atgatcaaaa atgatctcag
    13921 tttcaaactt ggtggcatgt tcgtgcatgc gctccattaa taacggaccg gtcagatcgt
    13981 ttggatcacc aggccagttt tccacttccg tggtggtggt cagttggccg cctttttcca
    14041 tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc gtagacagca gcggtgtatc
    14101 ccgccgggcc tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc atgagatccc
    14161 catagttgtt ggcagacaat gggcaggatt gtagggaatt tacagacgta aaaaaagagt
    14221 atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc
    14281 cgtcattatc acctctgcta cttaaatttc ccgctttata agccgattaa atgatgaata
    14341 aacgcccctg ttaatgaata tctggcatgt tgtactaaaa atcgatgttt tgctttgaca
    14401 atcccctggt gttttgcgaa aacattcgag gaagaaaaaa aacagtattc ttatatgcgc
    14461 ataaccatgc atgtaaatac catgtttacc gtgctagtga aatctacgta tggcgtggac
    14521 agacgccatt cgtgatgtcg atagctgcca caaggcaacg gtcttctcac cgtagaccca
    14581 ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg tgacgggtag cgactctgaa
    14641 cagtgatgtt tcaggggcag acaggagtag ggaaggaata cagagagaca ataataatgg
    14701 tagatagcaa gaagcgcctt ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg
    14761 agttgcaaaa ggatgggcgt atttctaacg tcgagctttc taaacgtgtg ggactttccc
    14821 caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca agggtttatt cagggctata
    14881 cggcgctgct taacccccat tatctggatg catcacttct ggtattcgtt gagattactc
    14941 tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac cgctgtacaa aaacttgaag
    15001 aaattcagga gtgtcattta gtatctggtg atttcgacta cctgttgaaa acacgcgtgc
    15061 cggatatgtc agcttaccgt aagttgctgg gggaaaccct gctgcgtctg cctggcgtca
    15121 atgacacacg gacatacgtt gttatggaag aagtcaagca gagtaatcgt ctggttatta
    15181 agacgcgcta acacggaaca ggtgcaaaat cggcgtattt tgattacact cctgttaatc
    15241 catacagcaa cagtactggg gtaacctggt actgttgtcc gttttagcat cgggcaggaa
    15301 aagcctgtaa cctggagagc ctttcttgag ccaggaatac actgaagaca aagaagtcac
    15361 attgacaaag ttaagtagcg gccgccgcct tctggaggcg ttgctgatcc ttattgtcct
    15421 gtttgccgtc tggttgatgg ctgccttact aagctttaac ccttcggacc ccagctggtc
    15481 gcaaacggcc tggcatgaac ctatccataa tttaggtggg atgcccggtg cgtggttggc
    15541 agatacgctg ttctttattt ttggcgtgat ggcttacacc attcccgtca ttattgtcgg
    15601 cggttgttgg tttgcctggc gtcatcagtc cagcgacgaa tacattgatt attttgccgt
    15661 ttcgctacgc atcattggcg ttttggcgct catccttacc tcctgtggtc tggcggcaat
    15721 caacgctgac gatatctggt attttgcctc cggtggcgtc attggcagct tactaagcac
    15781 tacgctacaa ccactgctac acagtagcgg gggaactatt gcgctgctct gcgtttgggc
    15841 agcgggcctg acgttgttca ccggttggtc atgggtgacc attgctgaaa aactcggcgg
    15901 ctggatttta aacattctca ccttcgccag taatcgtacc cgtcgcgatg atacctgggt
    15961 cgatgaagat gagtatgaag acgacgaaga gtatgaagat gaaaatcacg gcaaacagca
    16021 tgaatcacgc cgtgcccgta ttcttcgcgg cgcgctagcg cgtcgtaaac ggttggcgga
    16081 aaaattcatt aatccgatgg ggcggcaaac agacgctgcg ttgttctccg gtaagcggat
    16141 ggatgatgac gaagagatta cctacactgc acgcggtgtg gctgccgacc ctgacgacgt
    16201 cctattttcg ggcaatcgtg caacgcagcc agaatatgac gaatacgatc cattattaaa
    16261 cggtgcgcca attaccgaac ctgtcgctgt agcagctgct gctaccacgg cggcacaaag
    16321 ctgggctgcg ccggttgaac ctgtgactca gacgccgcct gttgcctctg ttgatgttcc
    16381 accttcgcaa cctacagtag cctggcagcc tgtaccgggt ccacaaacgg gagagccggt
    16441 tattgctcct gcaccggaag gttacccaca gcagccacaa tatgcgcagc ctgcagtgca
    16501 atataatgag ccgctgcaac aaccagtaca gccacagcag ccgtattatg cacctgcagc
    16561 tgaacaacct gtgcaacaac cttactacgc tcctgcggcg gaacaacctg ttcagcaacc
    16621 gtattacgcc cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca
    16681 gcaatccact tttgctccac agtctacata ccagactgag caaacttatc agcaaccagc
    16741 cgctcaggag ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc
    16801 tgaacccgtt gtagaagaga caaaacccgc gcgtccgcca ctttactatt ttgaagaagt
    16861 ggaagagaag cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga
    16921 accggttaaa gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt
    16981 acctccagta gaaaccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac
    17041 actggcgacg ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg
    17101 tggaccgcgt ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat
    17161 ccgcgtgcca actcgccgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc
    17221 ggcggaagaa aaagcccgtg aagcccagcg caatcagtac gattctggcg atcagtacaa
    17281 cgatgatgaa atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca
    17341 gcagcaacgc tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga
    17401 tgctgcggca gaggctgaac tggctcgtca gtttgcgcaa actcaacaac aacgttattc
    17461 cggcgaacaa ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat
    17521 gaaagcgttg ctggatgatg gtccacacga accgttgttt acgccaattg ttgaacctgt
    17581 acagcagccg caacaaccgg ttgcaccgca gcagcagtat cagcagccac aacagccggt
    17641 tgcgccacag cagcaatatc agcagccgca acagccggtt gcgccgcagc cacaatatca
    17701 gcagccgcaa caaccggttg cgccacagca gcaatatcag cagccgcaac aaccagttgc
    17761 gccgcagccg cagtatcaac agccgcaaca accggttgcg ccgcagcagc agtatcagca
    17821 gccacaacag ccagttgcgc cacaaccgca ggataccctg cttcatccgc tgttgatgcg
    17881 taatggcgac agccgtccgt tgcataaacc gacgacgccg ctgccttctc tggatttgct
    17941 gacaccgccg ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggctcg
    18001 cctggtggaa gcgcgtctgg ctgatttccg tattaaagcc gatgttgtca attactctcc
    18061 ggggccggtt atcactcgct ttgaattgaa cctggcaccg ggcgtaaaag cggcgcgcat
    18121 ttctaacttg tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga
    18181 agttattcct ggcaaaccct atgtaggtct ggagttaccg aataaaaaac gacaaaccgt
    18241 ttatctgcgc gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt
    18301 ggtgctgggt aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca
    18361 cttgttggtt gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct
    18421 gagcatgctt tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat
    18481 gctggagctt tcggtttatg aaggcattcc gcatctgtta acggaagtcg ttactgatat
    18541 gaaagatgcc gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc gttataaact
    18601 gatgtctgcg ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga
    18661 tcgcatgatg cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca
    18721 gcatccggtg ctgaaaaaag aaccatacat tgtggtgttg gttgacgaat ttgccgacct
    18781 gatgatgacg gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg
    18841 tgccgcgggt atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg
    18901 tctgattaaa gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc
    18961 acgtaccatt cttgatcagg ctggcgcgga atcactgctg gggatggggg atatgctcta
    19021 ctctgggccg aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga
    19081 agttcatgcc gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat
    19141 cacttccgac agtgaaagcg aaggtggcgc gggcggtttc gatggcgctg aagaactgga
    19201 tccgttgttc gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg
    19261 cgtacagcgt cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga
    19321 agcgcagggg attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggctccacc
    19381 gccgtttgac taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct
    19441 atcaactgaa aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag
    19501 agtagaggga actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg
    19561 ccatcacctg tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa
    19621 gcgatctgaa aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag
    19681 tgactgacgg tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc
    19741 caaacttatt caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta
    19801 aaacactgtg gttctataac ccgttcgttg agcaagctac ggcaacttgg ctgaaagatg
    19861 ccaccggtaa tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt
    19921 acaatatcaa acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc
    19981 tgaagcagtt caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg
    20041 agcaggacga tcagcgcagc agttatcaac tgaaatccca gcaaaatggg gctgtggatg
    20101 cagcgaaatt taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga
    20161 ggcacctgag tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc
    20221 gcgcgtatgc ggccagaaaa tttagcacag tatatcggcc agcaacattt gctggctgcg
    20281 gggaagccgt tgccgcgcgc tatcgaagcc gggcatctgc attctatgat cctctggggg
    20341 ccaccgggta ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat
    20401 gtggaacgta tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc
    20461 gcccggcaaa accgcaatgc aggtcgccgc actattcttt ttgttgacga agtgcaccga
    20521 ttcaacaaaa gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt
    20581 attggcgcaa ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc
    20641 cgtgtctatc tgttgaaatc cctgggtaca gaggatattg agcaagtact aactcaggcg
    20701 atggaagaca agacccgtgg ttatggtggt caggatattg ttctgccaga tgaaacacga
    20761 cgcgccattg ctgaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg
    20821 atggcggata tggccgaagt caatgatagc ggtaagcggg tcctgaagcc tgaattattg
    20881 accgaaatcg ccggtgagcg aagcgcccgc tttgataaca aaggcgatcg cttttacgat
    20941 ctcatttccg cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctctactgg
    21001 tatgcgcgaa ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ttgcctggca
    21061 attgcttccg aagatgtcgg caatgccgac ccacgggcga tgcaggtggc aattgcggcc
    21121 tgggattgct ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt
    21181 gtttacctgg cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agctgcgctg
    21241 gcggatgctc gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg
    21301 aaattaatga aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac
    21361 gcttatgctg ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc
    21421 ccgacaaaca ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag
    21481 gatcaaaata gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact
    21541 gtatccctgt ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata
    21601 agcatgctcg atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca
    21661 cgccggggct ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta
    21721 ttgcaggtca aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc
    21781 caggcgaaag cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc
    21841 gaagagctgg atgcagcaaa agccgagctg gatgctttac aggctgaaat tcgcgatatc
    21901 gcgctgacca tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac
    21961 aacgttgaag tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac
    22021 gtgacgctgg gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt
    22081 tcccgctttg tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt
    22141 atgctggatc tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt
    22201 aaccaggaca cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat
    22261 actcgtccgc tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa
    22321 gttccgctga ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag
    22381 atgaccgccc acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt
    22441 ggtctgatcc gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccggaa
    22501 gactcaatgg cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg
    22561 ggcctgccgt accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa
    22621 acttacgacc tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc
    22681 tccaacgttt gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac
    22741 aagaaaaccc gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg
    22801 gttgcagtaa tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg
    22861 cgtccgtata tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa
    22921 agcgcctgcg ggcgcttttt ttgtctccct ttgataccga acaataatta ctcttcactt
    22981 acacgtaata ctactttcga gtgaaaatct acctatctct ttgattttca aattattcga
    23041 tgtatacaag cctatatagc gaactgctat agaaataatt acacaatacg gtttgttact
    23101 ggaatcaatc gtgagcaagc ttgagtgagc cattatgaaa acgaaaatcc ctgatgcggt
    23161 attggctgct gaggtgagtc gccgtggttt ggtaaaaacg acagcgatcg gcggcctggc
    23221 aatggccagc agcgcattaa cattaccttt tagtcggatt gcgcacgctg tcgatagcgc
    23281 cattccaaca aaatcagacg aaaaggttat ctggagcgcc tgtacagtta actgtggtag
    23341 tcgctgcccg ctacgtatgc acgtcgtgga cggtgaaatc aaatatgtcg aaacggacaa
    23401 taccggcgat gacaattacg acggcctgca ccaggttcgc gcctgcctgc gtgggcgttc
    23461 catgcgtcgc cgtgtctaca atccggaccg cctgaaatat ccgatgaaac gagtcggggc
    23521 gcgcggtgaa ggcaaattcg agcgcattag ctgggaagaa gcctacgaca tcatcgcgac
    23581 caatatgcag cgcctgatca aagagtacgg caacgagtct atctatctga actatggcac
    23641 cggtacgctg ggcggcacca tgacccgctc ctggccgccg ggaaataccc tggtcgcgcg
    23701 gctgatgaac tgctgcggcg gctatctgaa ccattacggc gactactcct ccgcgcaaat
    23761 tgcggaaggt ttgaactata cctacggcgg ctgggcagat ggcaacagcc cgtcggatat
    23821 cgaaaacagt aagctggtag tgctgtttgg taataaccct ggcgaaacgc gaatgagtgg
    23881 cggtggggtg acttactatc ttgaacaggc acgccagaaa tctaatgccc gcatgatcat
    23941 catcgatccg cgctataccg acaccggtgc cgggcgcgaa gatgagtgga tccctattcg
    24001 tccgggaaca gatgccgcac tggttaacgg tctggcgtac gtcatgatca ctgaaaacct
    24061 ggtggatcag gcattcctcg ataaatattg cgttggctac gatgagaaaa ccctgccagc
    24121 cagtgcgccg aaaaatggcc actataaagc ttatattctg ggtgaagggc cagatggcgt
    24181 ggctaaaaca ccggaatggg cctcacaaat caccggtgtt ccggcagaca aaatcatcaa
    24241 actggctcgt gaaatcggca gtaccaaacc ggcgtttatc agccagggat ggggcccgca
    24301 gcgtcacgct aacggtgaaa tcgcaacccg tgctatctcg atgctggcga ttctgaccgg
    24361 taacgttggt attaatggag ggaacagcgg cgcgcgtgaa ggttcataca gcttaccgtt
    24421 tgtccgtatg ccgaccttgg aaaacccgat ccagaccagc atttcgatgt ttatgtggac
    24481 cgatgccatt gaacgtggcc cggaaatgac ggcgctgcgt gatggtgtac gcgggaaaga
    24541 taagctggat gtgccgatca aaatgatctg gaactatgcc ggtaactgcc tggttaacca
    24601 gcattctgaa atcaaccgta cccatgaaat ccttcaggat gataagaagt gcgagctgat
    24661 tgtggttatc gactgccaca tgacctcatc ggcgaaatat gctgacatcc tgctgcctga
    24721 ctgcaccgct tccgaacaga tggactttgc gctggatgca tcctgcggga atatgtctta
    24781 cgtgattttc aacgatcagg tgattaaacc gcgctttgaa tgtaagacca tctatgaaat
    24841 gaccagcgaa ctggcaaaac gtcttggcgt tgagcaacag tttactgaag gccgtaccca
    24901 ggaagagtgg atgcggcatc tgtatgccca gtcgcgggaa gcgattcctg aactgccaac
    24961 gtttgaagag ttccgcaagc aggggatctt taaaaagcgc gacccacaag ggcatcacgt
    25021 tgcttataaa gccttccgtg aagatccgca ggcaaaccca ctgactacgc catcgggcaa
    25081 aattgagatt tattcgcagg cgctggctga cattgccgct acctgggaat tgccagaagg
    25141 cgatgtgatc gatccactgc cgatctacac gccgggcttt gaaagttatc aggatccgct
    25201 gaacaaacag tatccgctgc agcttacggg tttccactat aaatctcgcg ttcactcaac
    25261 ttacggcaac gttgatgtgc tgaaagcagc ttgccgtcag gaaatgtgga tcaacccgct
    25321 tgatgcgcaa aaacgcggta tcaacaacgg cgataaagtc aggatcttta acgatcgtgg
    25381 tgaagttcat attgaggcga aagtgacgcc acgaatgatg ccaggtgtgg tcgcactggg
    25441 tgaaggtgcc tggtatgacc cggatgcaaa acgtgtcgat aagggtggtt gtattaacgt
    25501 actgaccact caacgtccgt ctcctctcgc taaggggaat ccgtcacata caaaccttgt
    25561 tcaggttgaa aaggtgtaag gagtaaccga tgacaaccca gtatggattt tttattgatt
    25621 ccagccgttg caccggttgc aaaacctgcg agctggcttg taaagactac aaagatttga
    25681 cgccagaagt cagcttccgc cgcatttatg aatatgctgg cggcgactgg caggaagata
    25741 acggtgtctg gcaccagaac gtgtttgcct actatctgtc gatttcgtgc aaccactgcg
    25801 aagatccggc ttgtactaaa gtctgcccga gcggtgcgat gcataaacgt gaagatggtt
    25861 ttgttgtggt cgatgaagat gtgtgcattg gctgccgcta ctgccatatg gcttgcccgt
    25921 atggcgcacc gcaatataac gaaacgaaag gccatatgac caaatgcgat ggttgttatg
    25981 accgtgttgc ggagggtaaa aagccgatct gtgttgaatc ttgtccgctg cgggcgctgg
    26041 atttcggccc tatcgacgag ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt
    26101 tgccgcgagc tcactttacc aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc
    26161 cgaccgggga taccactggc tatctggcaa acccgaagga ggtgtgagat gggaagtgga
    26221 tggcatgaat ggccgctgat gatcttcacg gtcttcgggc aatgtgtggc gggtggtttt
    26281 atcgttctgg ctttggcgct gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt
    26341 atcgcctgca tgtttggctt atgggtgctg atgggcattg gctttatcgc ttctatgctc
    26401 catcttggtt caccgatgcg cgcttttaac tcgctcaacc gggtaggggc ttcagcactc
    26461 agtaacgaaa tcgccagcgg ttcgatcttt tttgccgtag gcggcatcgg ctggttgctg
    26521 gcaatgctga aaaagctgtc accggcattg cgtacgcttt ggctggtggt gacgatggtt
    26581 cttggcgtcg tctttgtctg gatgatggtg cgtgtgtata acagcattga taccgttccg
    26641 acctggtaca gcatctggac gccgatgggc ttcttcctga cgatgttcat gggcggcccg
    26701 ttactggggt acctgttgct gagtctggct ggcgtcgata gctgggcgat gcgtctgctg
    26761 ccagccattt ccgtactggc actggtggtg agtggcgtgg tgtcagtgat gcagggcgca
    26821 gagctggcga ctattcatag ttctgtgcag caggccgcag cgctggtgcc ggactatggt
    26881 gcgctgatgt cctggcggat cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca
    26941 cagctaaaag gttatcagcc tgcggttccg ctactatccg tctcattcat tctgcttctg
    27001 gcaggggaat taatcggtcg cggcgtattc tatggtttgc atatgaccgt ggggatggcc
    27061 gtcgcaagct aatcagtaac aaccggggtt tcggccccgg ttctctttta tttctgcttc
    27121 gttaacgtgt cataactggt catcaaatta cgataatcag gaatatggtt agagaacaac
    27181 gtcgccagtc cttcaatatc attacgccag tcacggtgca gctcgcaagc cacgccgaac
    27241 catgtcatca attgcgcacc tgcttgcgac atgcgatccc atgccgaatg ccgggtaatt
    27301 tcattaaagg tccctgacgc gtcagtcaca acaaacacat caaacccttc ttcaatggct
    27361 gaaagcgccg ggaatgccac gcaaacttcg gttaccacac cggcaataat taactgtttt
    27421 ttacctgtcg ctttgactgc ttttacaaaa tcttcgttat cccaggcgtt aatattgccc
    27481 gggcgagcaa tgtaaggtgc atcggggaat tgtgctttca gttctggaac taatgggccg
    27541 ttaggaccgg tttcaaaact ggtggtaaga atagttggta agttgaaata cttagccagg
    27601 tcacccagcg ccagcacgtt atttttaaac ttatcgggtt cgatatcccg tacaagggaa
    27661 agtaaaccag cctggtgatc aacaagcaaa acagcagcat catttttatc aagacgaaca
    27721 tacggtttgg tcatcctctt ctcctttcga gagtcagtgg tacgcctgaa atcggtcaga
    27781 cgtgatgcat tgctctgaaa gcatagacgg gaaatatgag tttgctgtga ccatgaaatt
    27841 tttcgactga acgaagcgta tgaaatttgt gttagatcaa taaaaacaat cagatgagat
    27901 taatcatctc ttcagtatta gcgttatgaa tgagtaatat aaatcgataa taccgccgtt
    27961 gcgatatgcg cgtattttca cccattgaca atgtttttgg cggtggcatg atgcgcatga
    28021 aatttgaact tcctcacggt tttaattcat gtccacgtat acccggcctg tcatgctttt
    28081 gctgtctggc ctgcttttgt tgactctggc gatcgcggtg ttaaatacac tcgtgccgct
    28141 ttggctcgcc caggaacaca tgtccacatg gcaggtaggc gttgtcagct catcctattt
    28201 taccggcaac cttgtcggta cattgctgac agggtatgtc attaaacgca ttggttttaa
    28261 ccgcagctat tatctggcct ccttcatttt tgccgctggc tgtgccggcc ttggcctgat
    28321 gattggattc tggagttggt tggcgtggcg ttttgtcgcg ggcgtcggct gtgccatgat
    28381 ttgggtagtc gttgagagcg cgctgatgtg cagtgggacg tcacgtaacc gtgggcgttt
    28441 gcttgctgca tacatgatgg tttattacgt gggaacgttt ttaggccagt tactggtcag
    28501 caaggtctca accgagctga tgagcgtatt gccgtgggtt acaggtttga cattggcagg
    28561 gatcttaccg ctgttgttta cgcgtgtcct gaatcagcag gctgaaaacc atgattcgac
    28621 gtcaattacg gcaatgctaa aactccgtca ggcgcggctt ggcgtgaatg gctgcattat
    28681 ctcaggaatc gttctgggat ctctatatgg cctgatgcct ctgtacctca atcacaaagg
    28741 ggtgagcaat gccagtattg gtttctggat ggcggtactg gtcagtgcgg gtatcctcgg
    28801 acaatggccg attggacgtc tggcggataa gtttggtcga ctgctggttt tgcgtgttca
    28861 ggtctttgtc gtcattctcg gcagtattgc gatgcttagc caggcggcga tggccccagc
    28921 gttattcatc ctcggtgccg ctggctttac gctatatccg gtggcgatgg catgggcttg
    28981 cgagaaagtt gaacatcatc aactggtggc gatgaaccaa gccttactgt tgagctatac
    29041 tgtgggaagt ctgcttggcc cgtcatttac cgctatgcta atgcagaatt tctccgataa
    29101 tttattgttt atcatgatcg ccagcgtatc gtttatctat ttgctgatgc tgctgcgcaa
    29161 cgccggtcat acgccgaaac ccgttgctca cgtgtaaatg aattcaagca gagtgtgaac
    29221 ttactgtttc acactctgct tttttgtttc ttctatctga cttgctttat tccaaatttt
    29281 attcgtttaa aaataaaatg tgtagcaggt tataattttg catttcgcta tttccgcact
    29341 tcttatttgc cgcgcataat ccctcgtttt acagatgccc ctataatttt ggcgaaggat
    29401 ttgtctatgg ctgggaatgt tcaggaaaaa cagctgcgat ggtacaacat tgcgctgatg
    29461 tcttttatca ctgtctgggg ttttggcaac gttgttaata actacgccaa ccaggggctg
    29521 gtggttgttt tttcatgggt gtttatcttt gcgctctatt tcacacctta tgcgctaatt
    29581 gttggtcagt taggctcgac cttcaaagat gggaagggcg gggtcagtac ctggattaaa
    29641 cacacgatgg gacccggact ggcttatctc gccgcgtgga cctactgggt ggtgcatatt
    29701 ccctatctgg cacaaaaacc ctaggcaatt ctgattgcgc tcggttgggc gatgaaaggc
    29761 gacggttcgc taatcaaaga atattcagtc gtagcgttac aggggttaac gctggtgctg
    29821 tttatcttct ttatgtgggt tgcttcacgc ggtatgaaat cgctgaaaat cgtcggttct
    29881 gtggcaggga ttgctatgtt tgttatgtcg ctcctgtatg tggcgatggc ggtaaccgcg
    29941 cctgcaatta ctgaagtgca tattgcgacc acaaacatta cctgggaaac gttcattcct
    30001 catatcgact ttacctacat taccactatt tcaatgctgg ttttcgcggt tggcggagca
    30061 gagaagattt ctccttacgt taatcaaacg cgcaacccag gaaaagaatt tccaaaaggg
    30121 atgttatgcc tggcggtgat ggttgcggtt tgtgccattc tgggctcgct ggcgatgggg
    30181 atgatgtttg attcgcgtaa tatcccggat gacttaatga ccaacggtca gtattacgcc
    30241 tttcagaagc tgggcgagta ttacaacatg ggtaatactt taatggtgat ttacgccatt
    30301 gcgaataccc tgggacaagt agcggcgctg gtattctcga ttgatgcccc gcttaaagtg
    30361 ctattaggtg atgctgacag caaatatatt ccagccagtt tatgtcgtac caacgcttct
    30421 ggtacgcccg ttaatggcta ttttctgacc ctggtactgg tggcgattct gattatgctg
    30481 ccgactctcg gcattggtga tatgaacaat ctctataaat ggctgttgaa ccttaattcg
    30541 gtagtgatgc cgctgcgtta tttatgggta tttgttgcat ttattgcagt cgttcgcctg
    30601 gcgcagaaat ataaaccaga atatgtcttt attcgtaata agcctctggc aatgaccgtc
    30661 gggatttggt gttttgcctt taccgccttt gcctgtttga cggggatctt cccgaaaatg
    30721 gaagccttca ctgcagagtg gaccttccag ttggcgctga atgttgcaac gccgtttgtg
    30781 ctggtaggat taggactgat attcccgctg ctggcgcgta aagcgaatag taaataatta
    30841 ttgtggtcgg tgacgctgac tctggcagat ctgacccgtt tttacataat cgagaaatgc
    30901 gcgcaagccg caggacatat actggcggtt tgggtaatag atctggaagc ctggacgctc
    30961 tgtgatccag tcttccagta cacattctaa acgaccggta tcaaggtact ccttgatctg
    31021 ttcatagagc agatacccga taccagcacc catcaaaacg gcatccagtt cagcatcaac
    31081 atcat
//
LOCUS       DAJTYZ010000038        30871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000038 DAJTYZ010000000
VERSION     DAJTYZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.038"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..948
                     /gene="tuf"
                     /locus_tag="OO596_001667"
     CDS             <1..948
                     /gene="tuf"
                     /locus_tag="OO596_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6717539.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGD
                     NIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            1178..1561
                     /gene="secE"
                     /locus_tag="OO596_001668"
     CDS             1178..1561
                     /gene="secE"
                     /locus_tag="OO596_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6717540.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1563..2108
                     /gene="nusG"
                     /locus_tag="OO596_001669"
     CDS             1563..2108
                     /gene="nusG"
                     /locus_tag="OO596_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6717541.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2267..2695
                     /gene="rplK"
                     /locus_tag="OO596_001670"
     CDS             2267..2695
                     /gene="rplK"
                     /locus_tag="OO596_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6717542.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2699..3403
                     /gene="rplA"
                     /locus_tag="OO596_001671"
     CDS             2699..3403
                     /gene="rplA"
                     /locus_tag="OO596_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6717543.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3816..4313
                     /gene="rplJ"
                     /locus_tag="OO596_001672"
     CDS             3816..4313
                     /gene="rplJ"
                     /locus_tag="OO596_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6717544.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4380..4745
                     /gene="rplL"
                     /locus_tag="OO596_001673"
     CDS             4380..4745
                     /gene="rplL"
                     /locus_tag="OO596_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6717545.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            5065..9093
                     /gene="rpoB"
                     /locus_tag="OO596_001674"
     CDS             5065..9093
                     /gene="rpoB"
                     /locus_tag="OO596_001674"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6717546.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            9170..13393
                     /gene="rpoC"
                     /locus_tag="OO596_001675"
     CDS             9170..13393
                     /gene="rpoC"
                     /locus_tag="OO596_001675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6717547.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13635..14768)
                     /gene="thiH"
                     /locus_tag="OO596_001676"
     CDS             complement(13635..14768)
                     /gene="thiH"
                     /locus_tag="OO596_001676"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6717548.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14765..15535)
                     /gene="thiG"
                     /locus_tag="OO596_001677"
     CDS             complement(14765..15535)
                     /gene="thiG"
                     /locus_tag="OO596_001677"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6717549.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15537..15737)
                     /gene="thiS"
                     /locus_tag="OO596_001678"
     CDS             complement(15537..15737)
                     /gene="thiS"
                     /locus_tag="OO596_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6717550.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15721..16476)
                     /gene="thiF"
                     /locus_tag="OO596_001679"
     CDS             complement(15721..16476)
                     /gene="thiF"
                     /locus_tag="OO596_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6717551.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16469..17104)
                     /gene="thiE"
                     /locus_tag="OO596_001680"
     CDS             complement(16469..17104)
                     /gene="thiE"
                     /locus_tag="OO596_001680"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6717552.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17104..18999)
                     /gene="thiC"
                     /locus_tag="OO596_001681"
     CDS             complement(17104..18999)
                     /gene="thiC"
                     /locus_tag="OO596_001681"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6717553.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19080..19180)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19233..19709)
                     /gene="rsd"
                     /locus_tag="OO596_001682"
     CDS             complement(19233..19709)
                     /gene="rsd"
                     /locus_tag="OO596_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6717554.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19804..20577
                     /gene="nudC"
                     /locus_tag="OO596_001683"
     CDS             19804..20577
                     /gene="nudC"
                     /locus_tag="OO596_001683"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6717555.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMTEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20617..21681
                     /gene="hemE"
                     /locus_tag="OO596_001684"
     CDS             20617..21681
                     /gene="hemE"
                     /locus_tag="OO596_001684"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6717556.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21691..22362
                     /gene="nfi"
                     /locus_tag="OO596_001685"
     CDS             21691..22362
                     /gene="nfi"
                     /locus_tag="OO596_001685"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6717557.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22405..22995
                     /locus_tag="OO596_001686"
     CDS             22405..22995
                     /locus_tag="OO596_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6717558.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23182..23454
                     /gene="hupA"
                     /locus_tag="OO596_001687"
     CDS             23182..23454
                     /gene="hupA"
                     /locus_tag="OO596_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6717559.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23467..24162
                     /locus_tag="OO596_001688"
     CDS             23467..24162
                     /locus_tag="OO596_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6717560.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24164..24589)
                     /gene="zraP"
                     /locus_tag="OO596_001689"
     CDS             complement(24164..24589)
                     /gene="zraP"
                     /locus_tag="OO596_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6717561.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24827..26203
                     /gene="zraS"
                     /locus_tag="OO596_001690"
     CDS             24827..26203
                     /gene="zraS"
                     /locus_tag="OO596_001690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6717562.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            26200..27525
                     /gene="zraR"
                     /locus_tag="OO596_001691"
     CDS             26200..27525
                     /gene="zraR"
                     /locus_tag="OO596_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6717563.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27522..28811)
                     /gene="purD"
                     /locus_tag="OO596_001692"
     CDS             complement(27522..28811)
                     /gene="purD"
                     /locus_tag="OO596_001692"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6717564.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28823..30412)
                     /gene="purH"
                     /locus_tag="OO596_001693"
     CDS             complement(28823..30412)
                     /gene="purH"
                     /locus_tag="OO596_001693"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6717565.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7227 a         8035 c         8518 g         7091 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      721 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      781 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      841 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      901 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      961 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
     1021 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
     1081 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
     1141 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
     1201 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggt
     1261 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
     1321 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1381 cgttgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1441 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1501 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1561 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1621 gccgcgtagc aacgtcgcta cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1681 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1741 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1801 ggcacctggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1861 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1921 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1981 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     2041 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     2101 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     2161 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     2221 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     2281 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     2341 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2401 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2461 ttctttcact ttcgttacca agactccgcc ggcagcagtt ctgctgaaaa aagcggctgg
     2521 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2581 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2641 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2701 ggctaaactg accaagcgca tgcgtgttat ccgcgagaaa gttgatgcaa ccaaacagta
     2761 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2821 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2881 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2941 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     3001 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     3061 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgc ggcctgatgc caaacccgaa
     3121 agtgggtact gtaacgccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     3181 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     3241 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     3301 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3361 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3421 tgattttgtc tacaatctta cccccacata taatgcttaa tgcagacgta tatccgagat
     3481 attcgggttg tggcaaggcg gcaactgagt gagtcgccag gagcatagct aactatgtga
     3541 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3601 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3661 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3721 tgtttctgct caccgtaatt aagacgctct ctccgcttgg aggagtgaag tgagttccag
     3781 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3841 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3901 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3961 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     4021 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     4081 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     4141 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     4201 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     4261 gtaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     4321 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4381 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4441 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4501 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4561 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4621 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4681 aagacgacgc agaagcactg aaaaaatccc tggaagaagc tggcgctgaa gttgaagtta
     4741 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgactttttg
     4801 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4861 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4921 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4981 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     5041 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     5101 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     5161 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     5221 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     5281 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     5341 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5401 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5461 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5521 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5581 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5641 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5701 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5761 cgtgataaca agctgcagat ggagctggtg ccggaacgcc tgcgtggtga aactgcatct
     5821 ttcgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5881 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5941 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     6001 gcgaacatgg agctgagcct ggatctgttg gctaagctga gccagtctgg tcacaagcgt
     6061 attgaaacgc tgttcaccaa cgacctggat cacggcccgt atatctctga aaccttacgt
     6121 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     6181 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     6241 gaccgttatg acctgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     6301 gaaatcgaag gttccggtat cctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6361 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6421 atccgttctg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6481 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggacatg
     6541 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6601 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6661 ctcggcccgg gtggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6721 actcattacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6781 aactctttgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6841 aaggtgaccg acggtgttgt aactgacgaa attcactacc tttctgctat cgaagaaggc
     6901 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6961 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgtgacca ggttgactac
     7021 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     7081 cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc cgttccgact
     7141 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     7201 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     7261 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     7321 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7381 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7441 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7501 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7561 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg ggccggaaga gatcaccgct
     7621 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7681 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7741 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7801 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7861 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7921 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtttgttc
     7981 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     8041 ccgcgcgatc gctggctgga gctgggcctg accgacgaag agaaacaaaa tcagctggaa
     8101 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     8161 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggtg
     8221 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     8281 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     8341 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8401 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8461 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8521 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8581 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8641 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8701 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8761 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8821 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8881 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcacc
     8941 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     9001 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     9061 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     9121 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     9181 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     9241 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     9301 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9361 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9421 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9481 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9541 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9601 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9661 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9721 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9781 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9841 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9901 actgccgcca gatctgcgtc cactggttcc gctggatggt ggtcgtttcg cgacttctga
     9961 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
    10021 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
    10081 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
    10141 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
    10201 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
    10261 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
    10321 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10381 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10441 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10501 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10561 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10621 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10681 ggacgttgtt ctgggtctgt actatatgac ccgtgactgt gttaacgcca aaggcgaagg
    10741 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10801 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgcaaacg gtgaattagt
    10861 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10921 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10981 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    11041 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggta tcgatgacat
    11101 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    11161 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    11221 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    11281 aaccgtgatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    11341 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11401 tggtctgatg gcgaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11461 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11521 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11581 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11641 ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11701 tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca acacgctgct
    11761 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11821 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11881 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11941 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    12001 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    12061 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    12121 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    12181 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    12241 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    12301 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12361 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12421 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12481 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacaccc tggcgcgtat
    12541 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12601 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12661 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12721 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12781 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12841 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12901 gggcgttaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12961 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    13021 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    13081 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    13141 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    13201 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    13261 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    13321 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13381 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13441 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13501 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13561 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13621 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13681 cataccggct gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg
    13741 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13801 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13861 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13921 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13981 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    14041 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    14101 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    14161 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    14221 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    14281 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    14341 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14401 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14461 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14521 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14581 cgctgcgccc gttgggccag tggttccaga tagccactgg cggctggcga taacagcgcc
    14641 atcatatcgt cgcgggtgag ttgcgaggca tttagcgccc gttctacgtc agcagccgtt
    14701 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag
    14761 gttttcatgc cgatgcctcc agaaatccag tcagcgggct ggtggcatga gcaaaatgac
    14821 tgcggctgcc cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg
    14881 ccttcgccat gttgacggga tcgtccgcta cggcaatcgc cgtattcact aacaccgcgt
    14941 cggcccccat ttccagggcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa
    15001 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    15061 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    15121 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    15181 gcatttcggc ggctttcaga gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    15241 gaatctctaa ttttaaccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    15301 tggcttcttc cgctgttttc gccccggaag tatttggcag cagggtcaca cccgccgcga
    15361 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15421 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15481 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15541 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15601 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15661 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15721 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15781 caggctgcgc cactggctcg atttaccgtc gaacagtcgg agttctcccg caggtgtctc
    15841 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac
    15901 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag
    15961 gcagcggtaa cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc
    16021 gaccgcgctg gcggtgataa gcggcgtgtt gagtaccacg caggcggcat taatctcctg
    16081 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt
    16141 taacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatatcgg gattcaactg
    16201 tgtcagtcgc tgatggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag
    16261 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    16321 gacgccagcc cccgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16381 cagcacctgg ctgtcgagca gtttttgctg cccgtccaga gcgatatcgt cgagcagaat
    16441 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16501 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16561 tgccgacacc cgttgctatc accgcaggcg cgcgcgccag actgataccg ccaatcgcca
    16621 cggtgggata atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg
    16681 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16741 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16801 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16861 catacgcctg atgcttgatc gccagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16921 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca ttgcgccgat
    16981 ctttgatgcg tagctggagg gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    17041 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    17101 acatcacgct tcctccttac gcaggtagat ttctccgcct ctggcgcgga agttctccga
    17161 catatccgcc attcccactt caatggtttg cgcggcggcg taatcacgta cttcctggct
    17221 gattttcatc gagcagaatt tcggcccaca catggagcaa aaatgggcga ctttacctga
    17281 ctcttgcggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    17341 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17401 cgcgcctgga tgccctttcg ccaggtcagc ggcgtggacg gcaatcttat aggtgataag
    17461 cccctgctta acatcttctt tattaggcag acccagatgc tcttttggcg ttacgtaaca
    17521 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17581 atagcccggc gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17641 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc
    17701 ttcaatcatc acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg
    17761 cagctcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17821 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17881 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17941 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    18001 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    18061 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    18121 tgtaccgatc ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    18181 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    18241 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    18301 caggaaattg cgaccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18361 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18421 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18481 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18541 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18601 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18661 gcgcacggta agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc
    18721 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18781 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18841 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18901 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18961 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagtcaag
    19021 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    19081 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    19141 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    19201 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    19261 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    19321 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19381 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19441 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19501 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19561 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19621 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19681 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19741 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19801 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19861 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19921 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt agtacaacag
    19981 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    20041 caactggccg gacgaggcgt acaactggcg gagttttacc gatcgcataa atactgtggt
    20101 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg cagccattgc
    20161 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    20221 tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    20281 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    20341 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20401 ttaatgaccg cgtttatgac ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20461 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20521 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20581 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20641 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20701 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20761 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20821 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20881 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20941 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    21001 cgtaccattc gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    21061 tggacgctgg cgacctacat ggtggaaggc ggcagcagca aagcgttcac cgtgatcaaa
    21121 aaaatgatgt atgccgatcc gcaggcgctg cacgctctac tcgataaact ggcgaaaagc
    21181 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    21241 acctggggcg gtgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    21301 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21361 accaaaggcg gcggacagtg gctggaagcg atggcagaaa ccggttgcga tgcgttgggc
    21421 ctcgactgga caacggatat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21481 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21541 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21601 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21661 tctgaacaat atcaccgcta aggagtgatt atggatctcg cgtcattacg cgctcaacaa
    21721 atcgaactgg cttcttctgt gatccgcgag gatcgactcg ataaagatcc accggatctg
    21781 atcgccggag tcgatgtcgg gtttgagcag ggcggagaag tgacgcgagc ggcgatggtg
    21841 ctgctgaaat atccctcgct tgagctggtg gagtataaag ttgcccgcat cgccaccacc
    21901 atgccttaca ttccaggttt tctttccttc cgcgaatatc ctgcgctgct ggcagcgtgg
    21961 gagatgctgt cgcaaaagcc ggatttagtg tttgtcgatg gtcatgggat ctcgcatcct
    22021 cgccgtcttg gcgtcgctag ccattttggt ttgatggtgg atgtgccaac gatcggcgtg
    22081 gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc
    22141 ccactgatgg ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac
    22201 ccgttgttta tcgctaccgg ccatcgggtc agcgtggaca gcgcgctggc gtgggtacaa
    22261 cgctgcatga aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg
    22321 gaacgtccgg cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat
    22381 aatttccgta tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc
    22441 ctggaaagct ggcagcatgt cactttcatg gcttgcttat gcgaacgcat gtaccccaat
    22501 tacgccatgt tctgccagca aaccggtttt ggtgatgggc aaatttaccg tcgtattctc
    22561 gatctcatct gggaaacgct gaccgttaaa gacgcaaaag taaatttcga cagccaactg
    22621 gagaaatttg aagaagcgat tccttcagcc gacgatttcg atctgtacgg cgtttatccg
    22681 gcaatcgatg cctgcgtggc gttaagtgaa ctggtccatt cgcgtttgag tggtgaaacg
    22741 ctcgaacacg cggtggaagt gagtaagacc tccatcacga ccgttgcgat gctggaaatg
    22801 actcaggctg gtcgcgaaat gagcgatgaa gagcttaaag aaaacccagc tgtagagcaa
    22861 gaatgggaca ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc
    22921 gagctgataa aaggccttag ggcagacctg cgtgaggcgg gtgagagcaa tattggtata
    22981 atttttcagc aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc
    23041 ttccctttcg ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc
    23101 gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata
    23161 aacacattgt aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa
    23221 gcagaactgt ccaaaactca ggctaaagct gctctggagt ccactctggc tgcaattact
    23281 gagtctctga aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac
    23341 caccgcgctg agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca
    23401 gctaacgtac cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc
    23461 gtggcagtga acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga
    23521 tcattgatgc tggctggcgc tctgcttctc actgcctgta gtcataactc ttcacttcct
    23581 ccctttaccg ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac
    23641 agcggcgata atgtgcatct gcttgccgtg tttagcccgt ggcgcagtgg cgataccacg
    23701 acgcgagagt atcgctggca gggcgataac ctcacgctca tcaatatcaa tgtttacagc
    23761 aaaccgccgg tgaatattcg cgcgcgtttt gacgatcgcg gtgatctgag ctttatgcaa
    23821 cgtgaatccg atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat
    23881 cgtgctgatc agatccgcca gattagcgat gccttacgtc aggggagagt cgtgctgcgc
    23941 caggggcgct ggcatgcgat ggaacagacc gtgaccacct gcgaagggca aaccattaaa
    24001 cctgatttag attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt
    24061 gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc aattactgtt agtggcaaac
    24121 tctgatttct gtcgctggca acccaacgag aaaacgttct gatttaccag tggcccatac
    24181 ccatatgacc gccaccaccg cagccgccgt agcccatgcc cattccggct ccgcgcggaa
    24241 tacccgcttc agccatcgcg atatctcgtt tcacccgtaa ctcatctaac gactgacgca
    24301 aattctccat ctctttggcg accgcgttaa ttttgctgct atccggtggg ttcgcggcta
    24361 acagggcatt gtattcataa cgcttcgtca ccagttgctg ttgcagtgcg ctgctttgag
    24421 cgtaaaagtc attatggatt ttctgccacg ctgtctgttg ttcgctggtc aaaggcgcgg
    24481 cattttgctg ccacataccg tgtccgccgt gagcaaatgc agatgtcgat cccatcgcca
    24541 ttgctgaaag cgccatcatt accagggcaa ttttcgtgtt ccgtttcatg gttaatcctc
    24601 cagtggttgt cttacttcgg gtattgcatc ttccgtgcca acgatgaaac gctgatatga
    24661 cgggtaatct ggcatgataa acgagtaaaa atgactcgcc tgctgcgggt agcgagtcat
    24721 ttttactcat tgaaacttaa gcctttgtgt tacagcgcag ggtaagcgct gataaaagat
    24781 ggcatgattt ctgctgtcag aaagggatga gcaggcaaag aagaagatgc gttttatgca
    24841 acgttctaaa gactccttag ctaaatggtt aagcgcgatc ctccccgtgg tcattgttgg
    24901 gctggtaggg ttgtttgcgg tgacggtgat tcgcgattat gggcgcgaga ctgccgccgc
    24961 cagacaaacg ctgctggaaa aaggcagtgt acttattcgc gctcttgaat ccggctcgcg
    25021 cgtcggcatg gggatgcgca tgcatcatgc gcagcagcag gccttactgg aagaaatggc
    25081 cgggcagcct ggagtacgtt ggtttgcggt cacggatgaa caaggaacaa tcgtgatgca
    25141 tagcaactcc ggcatggtgg gaaaacagct ttattccccg caggaaatgc agcagttaca
    25201 tccgggagat gaagaagcgt ggcggcggat cgatagcgca gacggcgagc ctgttctgga
    25261 aatttatcgc cagtttcaac cgatgtttgc tgctggaatg caccggatgc gccatatgca
    25321 gcagtatgcc gcgacaccac aagcaatttt catcgctttc gacgccagta atattgtgag
    25381 tgccgaagat cgtgagcaga gaaacaccct gattatcctc ttcgccctgg cgacggtctt
    25441 gctggcaagc gtattgtcat tcttctggta tcgccgctat ttgcgctcgc gccagcttct
    25501 acaagatgaa atgaagcgca aagagaagct ggtggcgctg gggcatcttg cggcaggcgt
    25561 tgcccacgaa atccgtaacc cactttcctc gattaaagga ctggcgaaat actttgccga
    25621 gcgcgcgcct gcagggggag aagcgcatca actggcgcag gtgatggcga aagaggccga
    25681 ccgtttaaac cgcgtggtaa gcgagctgct ggaactggtt aagccaacgc atctggcttt
    25741 gcaggcggtg gatctcaaca cgctgattaa ccactcatta cagctggtaa gccaggatgc
    25801 gaacagccgg gagatccagt tacgctttac cgccaacgac acattaccgg aaattcaggc
    25861 cgatccggac aggctgactc aggtcctgtt gaatctctat ctcaatgcta ttcaggcgat
    25921 tggtcagcat ggcgtgatta gcgtgacggc cagcgaaagc ggcgcgggcg tgaaaatcag
    25981 cgttaccgac agcggtaagg gaattgcggc agatcagctt gatgccatct tcactccgta
    26041 cttcaccact aaagccgaag gcaccggact ggggctggcg gtcgtgcata atattgttga
    26101 acaacacggt ggtacaattc aggtcgcaag ccaggaggga aaaggcgcaa cgttcaccct
    26161 ctggcttccg gtcactatta cgcgtaaggg cccacaagga tgacgcacga taatatcgat
    26221 attctggtgg tggatgatga cattagccac tgcactattt tgcaggcttt actgcgcggc
    26281 tggggctata acgtcgcgct ggcgaacagc gggcgacagg cgctggagca ggtgcgggaa
    26341 caggtttttg atcttgtgct ttgcgatgtg cgaatggcgg agatggacgg cattgccacg
    26401 ctaaaagaga tcaaagcgtt aaatccggcg attccggtgc tgattatgac cgcatactcc
    26461 agcgtcgaga cggcggtaga agcgttgaaa accggggcgc tggattatct catcaagccg
    26521 ctggatttcg ataacctgca ggcgacgctg gaaaaggcgc tcgcgcatac gcacagtatt
    26581 gacgctgaaa cgcctgcggt gtctgccagc cagttcggta tggtcggtaa aagcccggcg
    26641 atgcaacacc tgctcagtga aatcgccctc gtcgcgccat cggaagccac ggtgctgatc
    26701 cacggcgatt ccggcaccgg taaagagctg gtcgccaggg cgattcacgc cagtagcgca
    26761 cgtagcgaaa aaccgctggt aacgctcaac tgtgcggcac tcaacgaatc cttgctggaa
    26821 tctgaattgt tcggtcacga aaaaggggcg ttcactggag ccgatagacg ccgggaaggg
    26881 cgctttgttg aggcggacgg cggcacgctg tttctcgatg aaattggcga tatctcgccg
    26941 atgatgcagg tgcgtctgct gcgtgcgatt caggagcgcg aagttcagcg tgtcggtagc
    27001 aaccagacta tctcggttga tgtccggttg attgcggcga cccatcgcga tcttgccgca
    27061 gaggtgaatg ccgggcgttt tcgccaggat ctctactatc gcctgaatgt ggtggcgatt
    27121 gaagtaccat cgctgcggca acggcgggaa gatatccctc tgctggctgt ccattttctt
    27181 cagcgctttg ccgagcgtaa tcgcaaggcg gtaaaaggct ttacgccaca ggcgatggat
    27241 ttgctgatcc actacgactg gccgggaaat attcgtgaac tggaaaacgc ggtggaacgg
    27301 gcggtggtgc tgctgaccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc
    27361 ggtacgccga tcccactggg gcaaagtcag gatattcagc cgctggtgga agtcgaaaaa
    27421 gaagtgattc tggcggcact ggagaaaacg ggcggcaaca aaaccgaagc cgcccgtcag
    27481 ttagggatca cgcgcaaaac gctattggca aaactgtcgc gttagttctg ctcgcgttcg
    27541 atagcgcgcc agccgatatc tttccggcag aagcagtcgt cccagtgaat atcggtcatt
    27601 aaggcataag cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt
    27661 acgcgcccgc cgctggtcac tacctgctcg ttatccgcca gtttcgtgcc cgcgtggaac
    27721 actttgccgc ctgccacttc ttccagcggc aggccgtgga tcacatcgcc ggtgcggtaa
    27781 tcgcccggat atccacccgc agccatcacc acgccgagag aagcacgctc atcccactct
    27841 gacgttttct cgtccagttt gccttcacag gccgccaggc agagctcaac cagatcggac
    27901 ttcatgcgca gcataatcgg ctgggtttct ggatcgccaa agcggcagtt aaattcgata
    27961 accttcggat tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg
    28021 ttgccttccg ccgccatgcc tttcacggtt ggccagatga tacgttccat ggtgcgctga
    28081 tgaacttcat cggttactac cggcgcgggg gagtaagcgc ccatcccgcc cgtgtttggt
    28141 ccggtatctt tatcgcctac gcgtttgtga tcctggctgg tggccatcgg cagcacatgc
    28201 tcgccgtcta ccatcacgat aaagctcgct tcttcgccgt cgaggaactc ttcgataacg
    28261 atgcgatgac ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcg
    28321 gcttcttcca gcgtcatcgc cacgataacg cctttcccgg ctgccagacc gtccgcttta
    28381 atgacgatcg gcgcaccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag
    28441 ttctggtatt ccgctgtagg gatcttatgg cgggccagga aatctttggt aaacgctttc
    28501 gaaccttcca gttgcgcggc acctgcggtt gggccgaaga ttttcatccc ggcggcgcgg
    28561 aaggtatcga ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc
    28621 ttttcgtttt gtgcgaaatc caacagcgcc gggatatcgg tcacgccgat agcaacgttt
    28681 tgcagcgcgg gttccagcgc agtgcctgca ttacccggag caacaaaaac agtttcaacc
    28741 agcggcgact gcgccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact
    28801 aatactttca tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg
    28861 caataccgtg ctcgtcggcg gcggcaatca cttcgtcatc acggatagaa ccgccaggct
    28921 ggattacgca ggtcacgcct gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga
    28981 acgcgtcaga agccatcgag gaacctttca cttccaagcc ttcatcggca gctttaatac
    29041 cggcgatttt cgcggagtac acgcggctca tctggcccgc gccaatcccg atggtcatat
    29101 tgtttttggc atagacgata gcgttggatt tcacgaactt cgccactttc cagcagaaca
    29161 gcgcatcacg cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg
    29221 caccgaccat gcccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat
    29281 caagacccgg aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg
    29341 cggcggtgat tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact
    29401 gacgagaaat gatggcctgt gcggtttccg catccagctc gcggttaaag gcaatgatgc
    29461 cgccgaatgc ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat
    29521 tgccgatagc cacgccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga
    29581 actctttcac gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt
    29641 taccctgaac ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga
    29701 aggcagcctg ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca
    29761 ggttcagcgt gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg taagccggaa
    29821 ccatgctgcc gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt
    29881 tgatggcgag gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt
    29941 taataatggc gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag
    30001 cggcggagcg caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg
    30061 agcagccttc acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga
    30121 taggctggat ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc
    30181 caccatgtac tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg
    30241 tgtaatcgga aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc
    30301 ctgtagacag cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg
    30361 ctttgtcaga aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc
    30421 ccctggattt gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac
    30481 gcccctaacg gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc
    30541 tcgcgaattt ttctctttca atggtgatca caattttgac agtggttacc gtgggaaaaa
    30601 tacagaaatt acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt
    30661 tttgctgggg aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga
    30721 gaaatcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca cacaaacagc
    30781 cggttcggtt gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta
    30841 atatacgcca cctcgcgaca gtgcgctaaa g
//
LOCUS       DAJTYZ010000039        30004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000039 DAJTYZ010000000
VERSION     DAJTYZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.039"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            40..582
                     /locus_tag="OO596_001694"
     CDS             40..582
                     /locus_tag="OO596_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6717566.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            665..1366
                     /locus_tag="OO596_001695"
     CDS             665..1366
                     /locus_tag="OO596_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6717567.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1391..3990
                     /locus_tag="OO596_001696"
                     /pseudo
     CDS             1391..3990
                     /locus_tag="OO596_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3981..5051
                     /locus_tag="OO596_001697"
     CDS             3981..5051
                     /locus_tag="OO596_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6717568.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5063..5605
                     /locus_tag="OO596_001698"
     CDS             5063..5605
                     /locus_tag="OO596_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6717569.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5613..6128
                     /locus_tag="OO596_001699"
     CDS             5613..6128
                     /locus_tag="OO596_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6717570.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6094..6831
                     /locus_tag="OO596_001700"
     CDS             6094..6831
                     /locus_tag="OO596_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6717571.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6942..7952
                     /gene="pyrD"
                     /locus_tag="OO596_001701"
     CDS             6942..7952
                     /gene="pyrD"
                     /locus_tag="OO596_001701"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6717572.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8126..8668
                     /gene="zapC"
                     /locus_tag="OO596_001702"
     CDS             8126..8668
                     /gene="zapC"
                     /locus_tag="OO596_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6717573.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8665..9774)
                     /gene="ycbX"
                     /locus_tag="OO596_001703"
     CDS             complement(8665..9774)
                     /gene="ycbX"
                     /locus_tag="OO596_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6717574.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10018..12126
                     /gene="rlmKL"
                     /locus_tag="OO596_001704"
     CDS             10018..12126
                     /gene="rlmKL"
                     /locus_tag="OO596_001704"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6717575.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12138..14045
                     /locus_tag="OO596_001705"
     CDS             12138..14045
                     /locus_tag="OO596_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6717576.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14175..15428
                     /gene="pqiA"
                     /locus_tag="OO596_001706"
     CDS             14175..15428
                     /gene="pqiA"
                     /locus_tag="OO596_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6717577.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKATCPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15433..17073
                     /gene="pqiB"
                     /locus_tag="OO596_001707"
     CDS             15433..17073
                     /gene="pqiB"
                     /locus_tag="OO596_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6717578.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17070..17633
                     /gene="pqiC"
                     /locus_tag="OO596_001708"
     CDS             17070..17633
                     /gene="pqiC"
                     /locus_tag="OO596_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6717579.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17889..18056
                     /gene="rmf"
                     /locus_tag="OO596_001709"
     CDS             17889..18056
                     /gene="rmf"
                     /locus_tag="OO596_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6717580.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18126..18644)
                     /gene="fabA"
                     /locus_tag="OO596_001710"
     CDS             complement(18126..18644)
                     /gene="fabA"
                     /locus_tag="OO596_001710"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6717581.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18713..20473)
                     /locus_tag="OO596_001711"
     CDS             complement(18713..20473)
                     /locus_tag="OO596_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6717582.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20659..21111
                     /gene="matP"
                     /locus_tag="OO596_001712"
     CDS             20659..21111
                     /gene="matP"
                     /locus_tag="OO596_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6717583.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21187..22227)
                     /gene="ompA"
                     /locus_tag="OO596_001713"
     CDS             complement(21187..22227)
                     /gene="ompA"
                     /locus_tag="OO596_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6717584.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22582..23091)
                     /gene="sulA"
                     /locus_tag="OO596_001714"
     CDS             complement(22582..23091)
                     /gene="sulA"
                     /locus_tag="OO596_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6717585.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23310..23939
                     /gene="sxy"
                     /locus_tag="OO596_001715"
     CDS             23310..23939
                     /gene="sxy"
                     /locus_tag="OO596_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6717586.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23902..26064)
                     /gene="yccS"
                     /locus_tag="OO596_001716"
     CDS             complement(23902..26064)
                     /gene="yccS"
                     /locus_tag="OO596_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6717587.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26074..26520)
                     /locus_tag="OO596_001717"
     CDS             complement(26074..26520)
                     /locus_tag="OO596_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6717588.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26643..28697
                     /gene="helD"
                     /locus_tag="OO596_001718"
     CDS             26643..28697
                     /gene="helD"
                     /locus_tag="OO596_001718"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6717589.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28729..29187)
                     /gene="mgsA"
                     /locus_tag="OO596_001719"
     CDS             complement(28729..29187)
                     /gene="mgsA"
                     /locus_tag="OO596_001719"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6717590.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29283..29945)
                     /locus_tag="OO596_001720"
     CDS             complement(29283..29945)
                     /locus_tag="OO596_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6717591.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7734 a         7085 c         7880 g         7305 t
ORIGIN      
        1 aggcattatc atatttacga agagatcagg atgatgacga tgaaaaaaag tgtattgacg
       61 gcgtttataa ctgtggtatg tgcaacgtcc agcgttatgg cagctgatga taatgctatc
      121 acggatggca aagtaacatt taatggtaaa gttattgctc cagcttgtac cctggtagct
      181 gcgacgaaag attcagtagt cacattgcca aatgtcagcg ccaccaagtt gcaaactaat
      241 ggagcagtct ctggcgttaa aactgatgta ccaattgcct tagaaggctg tgatgttact
      301 gtaactaaaa atgcgacgtt tacctttagt ggtacggctg acggcgtgca acctacagca
      361 tttgccaacc aggctaccac cgatgcagcg acgaatgttg ctctacaaat gtatctgcca
      421 gatggttcta catcggtaac tcccggtacc gaaacgtcga atattcagtt agcggattct
      481 gctgagcaaa cagtgacctt taaagttgat tatatcgcta cggggaaagc gacttctggt
      541 aatgtgaatg cggtaacaaa tttccatatt aactattatt aatagaactc attaattgtt
      601 ttattaatta gtacccctct agtgttctgg aggggatatt catatttttt aagagtgact
      661 atttatgaaa acttgcataa caaagggaat tgtgaccgta agtttaacgg caatattact
      721 cagctgctca tccgcatggg cggcaggtaa aggcgggatt ggacttgcag caacacgtct
      781 tgtttattca gaaggtgaag agcaaatttc actgggtgta cgtaacacca gtccggatgt
      841 tccttatctt attcagtcat gggtgatgac cccagataat aaaaaatcag cagattttat
      901 tattacacca ccgctatttg tgctgaatcc ggctaatgag aatctgttac gcattatgta
      961 cattggtgcg ccgttggcga aagacagaga aacccttttc ttcactagcg tacgggcagt
     1021 cccttcaaca acgaagcggg aagagggaaa taccctgaag attgccacac aaagcgtcat
     1081 caaacttttc tggcgaccaa aaggtttagc gtatccctta ggcgaggctc cggcgaaact
     1141 gcgttgcact tcgtcagctg acatggttac ggtcagtaac ccaacacctt atttcattac
     1201 cctgacagac ctgaaaatag ggggaaaggt agttaaaaat caaatgattt ccccctttga
     1261 taaataccaa ttctctctgc caaagggggc caaaaatagc agcgtaacgt atcgaaccat
     1321 caatgattac ggggcggaaa cgccacaact caactgtaaa tcgtaagccg tcttcagtta
     1381 agagagcgag atgtatagaa ctcatcgaca acacagcctg ttaagctctg gtggagtgcc
     1441 atcgtttatt ggtgggctgg tggtgtttgt gtcggcagcg ttcaatgcac aagctgaaac
     1501 ctggttcgat cctgcttttt tcaaagatga tccctcaatg gtggccgatt tgtctcgttt
     1561 cgaaaaagga caaaaaataa cgccaggggt ttatcgtgtc gatattgttc tgaatcagac
     1621 aattgtagat acgcgcaacg tcaattttgt tgagataacg ccagagaagg ggattgccgc
     1681 ctgtttgacg actgaaagcc tggatgcaat gggtgttaat actgatgcgt ttccggcttt
     1741 taaacaactg gacaaacaag cgtgtgtgcc attggcggag attattccgg atgccagcgt
     1801 aacgtttaat gtgaataaac tccgtctgga aatttcagta ccgcaaatcg ccatcaaaag
     1861 taatgcccgt ggttatgtcc cccctgaacg ttgggatgaa gggatcaacg cgctattact
     1921 gggatattcg tttagcgggg ctaacagtat tcatagcagc gcagacagtg attctggcga
     1981 cagctatttt ctgaatttaa acagtggcgt taatttaggc ccatggagat tgcgcaacaa
     2041 ttcaacatgg agtcgcagta gtggccaaac cgcagaatgg aagaatctca gcagctattt
     2101 gcagcgggcg gttattccac taaaaggcga actgaccgta ggtgatgatt atactgcagg
     2161 cgattttttc gatagtgtca gctttcgtgg tgtgcagctg gcgtcagatg acaacatgct
     2221 gccagacagc ctgaaagggt ttgcgcctgt ggtgcgtggt atcgccaaaa gcaatgccca
     2281 gataacgatt aagcaaaatg gttacaccat ttaccaaact tatgtatcgc ctggtgcttt
     2341 tgaaattagt gatctctatt ccacgtcgtc gagcggtgat ttgttagttg aaatcaaaga
     2401 agcggacggc agcgtcaata gctacagcgt accgttttcc agcgtgccat tactccagcg
     2461 tcaggggcga atcaaatacg cggtgacact ggcgaaatac agaaccaata gtaatgaaca
     2521 gcaggagagc aaatttgcct aggccacgtt gcagtggggc ggaccgtggg gaacgacatg
     2581 gtatggtggt ggacaatatg ctgaatatta ccgtgccgcc atgtttggtc tgggatttaa
     2641 ccttggcgat ttcggagcaa tttcgttcga tgcgacccag gcgaagagta cgctggcaga
     2701 ccaaagcgaa cataaaggtc agtcatatcg ttttctgtat gccaaaacgc tcaaccaatt
     2761 gggcactaat tttcaattga tgggctatcg ctattcgacg tcgggtttct acaccctttc
     2821 cgacaccatg tataaacata tggatggcta cgaatttaat gacggtgatg atgaagatac
     2881 gccgatgtgg tcgcgttatt acaatttgtt ttacaccaaa cgtggcaaac tgcaggtcaa
     2941 tatctcccag caattaggcg agtacggttc gttttattta agtggtagcc agcaaactta
     3001 ctggcatacc gatcaacagg atcggctatt acagtttggc tacaacacgc aaattaaaga
     3061 tctctcgctg gggggttcct ggaactacag taagtcccgt ggtcaacctg atgctgatca
     3121 ggtgtttgca ctaaattttt ccctgccgct caatctgttg ctccccagaa gtaatgatag
     3181 ctataccagg aaaaaaatta cgcctggatg acctctaaca ccagcatcga taacgaaggg
     3241 cacattacac aaaacctggg tttaacggag acactactcg atgacggtaa cctgagctac
     3301 agcgtgcaac agggatataa cagcgagggg aaaacggcta atggtagcgc cagcatggac
     3361 tacaaagggg cgtttgcaga tgcccgagtg ggctacaact acagcgataa cggcagtcaa
     3421 caacaactga actacgctct ttcaggcagt ttagttgccc attcacaggg cattaccctg
     3481 gggcaatcgc tgggggaaac taacgtgctg attgcagcac caggcgcaga gaatactcgt
     3541 gtggcgaaca gcaccgggct gaaaactgac tggcgcggat ataccgttgt cccctatgcc
     3601 acttcttatc gggaaaatcg aatcgcactt gatgcggcat cgttaaaacg taacgtggat
     3661 cttgaaaatg cagtagtcaa cgtggttccc accaaagggg cgttggttct ggcggagttc
     3721 aatgcccatg cgggagcaag ggtattaatg aaaacatcaa agcagggtat accgctgcgt
     3781 tttggcgcga tagcgacgct ggacggcgta cagactaata gcggcattat tgatgatgat
     3841 ggctcgctct atatgtccgg tttgcccgct cagggagcga taactgtacg ctggggcgaa
     3901 gctcccgatc aaatttgtca tatcagttac cagcttaccg aacaacaaat taactctgcg
     3961 attacgcgaa tggatgccat atgcagataa tctttggaga aaaatgcgtg ttattactac
     4021 gactattttt tgccgccgtc ttaatgctat ggtgcgctca aactgctgct tatagcgggc
     4081 agtgtcatac cactcagggg aatccgtata ttggcgtcaa ttttggcgtt aaaaccctgg
     4141 aggaagaaga aaatacggct ggagtagtta aagacaaatt ttatcagtgg aacgaatcga
     4201 atgattatta tgtttcctgt gattgcgata aagacaatgt cagaagtggc cgatgggcat
     4261 tcgccgcgga ttcaccgtta gtctatttag gcgacaactg gtacaaaatt aatgactatc
     4321 ttgccgccaa agttttattg caggttaaag gcagttctcc tactgcggtt cctttcgaaa
     4381 acgtgggcac aggggcagat acacgatggc atatttgcga tcccggcggt caacgtttag
     4441 gtggccaggg ggctagcggt aatagcggta gcttttccct gaaaatattg cagccgttcg
     4501 ttggttcggt cgtcattcct cctatggcgc tggcgcgatt atttgaatgc tacaacatac
     4561 ccgcaggtga ttcctgcacg actacaggta caccggtttt agtgtattac ctgtctggta
     4621 cgatcaattc acttggctca tgttccgtca atgccggaga aacaatcgag gtcgatctgg
     4681 gcgacgcatt tgcggctaac tttcgtgttg tagggcataa gcctcttggg gccagaacgg
     4741 cagaacttgc aattccagtc aggtgtaaca cgggaaacgc ggggttagtt aacgtcaacc
     4801 tgagtctgac ggcaaccaca gaccccagct atccccaggc gattaagacg tcacgtcctg
     4861 gcgtgggcgt ggtggtgacc gatagccaga acaacattat ttcccctgct ggtggaacat
     4921 taccgctctc tattcctgat gatgcagaca gtatcgcgcg aatgaatgtc tatccagtca
     4981 gcacgacagg tgtaccacca gaaaccgggc gatttgaagc cacggcaacg gtgagaataa
     5041 attttgatta acgcgtgaac gtatgaagaa agaaacgata tttcagtgcg ttatcttgtt
     5101 ctttagcatt cttaacatcc atgtcgggat ggctgggcct gaacaagtta gtatgcatat
     5161 ttatgggaat gtggtcgatc agggctgtga tgtcgccacc aaaagtgcat tacaaaatat
     5221 tcatattggt gattttaata tcagtgattt tcaggccgcg aataccgtaa gcactgctgc
     5281 tgatttgaat atcgatatca ccggttgtgc cgctagtatt actggcgcgg acgtcctttt
     5341 tagcggcgag gctgacaccc ttacgccgac actgctcaaa ctaactgaca caggcggaag
     5401 cggtggtatg gcaacgggga ttgccgtgca aattcttgat gcgcaaagtc agcaagaaat
     5461 cccgctcaat caggtccagc ctcttacgcc cttaaaagcc ggggataaca cactcaaata
     5521 tcaacttcgt tataagtcca caaaggcggg agcaacgggc ggtaatgcga cggcggttct
     5581 ctattttgat ctggtttacc agtgaaggag tgatgttgaa acggataatc tggattctgt
     5641 tcttactggg attaacgggg ggctgtgagc tatttgccca tgatggcacg gtcaacatta
     5701 gcggatcgtt tcgccgtaat acatgcgtgc tggcacagga tagcaagcaa atcaatgtgc
     5761 agttaggcga tgtctcgctg acacgttttt ttcatgggaa ttatggcccg gagaaatctt
     5821 tcatcattaa tttgcaggat tgcggaacag atgtcagcac ggttgacgtc accttttcag
     5881 gaacccccga cggagtgcag agcgaaatgc tgtccattga aagtggtacg gatgcagcca
     5941 gcgggctggc aattgcgatt ctggatgatg cgaagatatt gattccgctt aaccaggcga
     6001 gtaaagacta cagcctacat agcggtaagg tgccattaac tttttatgcg caattgcgac
     6061 ctgtcaatag cgatgtgcag tcgggtaaag tgaatgccag cgcgacattt gtacttcatt
     6121 atgactaata cgtggaacag attggcgctc ttgattttcg ccgttttatc gctgctggtg
     6181 gcgggtgaat tgcgggcagg agtggtggtc ggcggaacgc gatttatctt tccggcagac
     6241 agagaatcga tatctatttt actgactaat accagtcagg aatcctggct tattaacagt
     6301 aaaatcaacc gcccaacgcg ttgggccggg ggggaagcgt cgacagtgcc agcaccatta
     6361 ctggccgctc cgccacttat tctcctgaag cccggtacta caggcacgtt gcgcttgctg
     6421 agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc tatttgagtt aagcattgcc
     6481 agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag tggcgatgcg ctcggtattt
     6541 aaactgttct ggcgacccga aggtttgccg ggcgacccgc tggaagctta tcaacaatta
     6601 cgctggacac ggaattcgca gggtgtacaa ctcactaacc caacgcctta ctacattaac
     6661 ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg cgggagttgt gccgcctaaa
     6721 agccagcgtc agacaagctg gtgtcaggca atcgcaccgt gtcatgtcgc ctggcgggcg
     6781 ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa atctgccctg aaacaggttc
     6841 ggaaaacgtt tgcgtttttt ttgccgcagg tcaattccct tttggtccga actcgcacat
     6901 aatacgcccc cggtttgcac accgggaatc caggagagtt catgtactac cccttcgttc
     6961 gtaaagccct tttccagctc gatccagagc gcgctcatga gtttactttt cagcaattac
     7021 gccgtattac aggaacgccg tttgaagcac tggtgcggca gaaagtgcct gcgaaacctg
     7081 ttaactgcat gggcctgacg tttaaaaatc cgcttggtct ggcagccggt cttgataaag
     7141 acggggagtg cattgacgcg ttaggcgcga tgggatttgg atcgatcgag atcggtaccg
     7201 tcacgccacg tccacagcca ggtaatgaca agccgcgtct ctttcgtctg gtagatgccg
     7261 aaggtttgat caaccgtatg ggctttaata atcttggcgt tgataacctc gtagagaacg
     7321 taaaaaaggc ccattatgac ggcgtcctgg gtattaacat cggcaaaaat aaagatacgc
     7381 cagtggagca gggcaaagat gactatctga tttgtatgga aaaaatctat gcctatgcgg
     7441 gatatatcgc catcaatatt tcatcgccga ataccccagg attacgcacg ctgcaatatg
     7501 gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa gcaaaatgat ttgcaagcga
     7561 tgcaccataa atatgtgccg atcgcagtga agatcgcgcc ggatctttct gaagaagaat
     7621 tgatccaggt tgccgatagt ttagttcgcc ataatattga tggcgttatt gcaaccaata
     7681 ccacactcga tcgttctctt gttcagggaa tgaaaaattg cgatcaaacc ggtggcttaa
     7741 gtggtcgtcc gcttcagtta aaaagcaccg aaattattcg ccgcttgtca ctggaattaa
     7801 acggtcgctt accgatcatc ggtgttggcg gcatcgactc ggttatcgct gcgcgtgaaa
     7861 agattgctgc gggtgcctca ctggtgcaaa tttattctgg ttttattttt aaaggtccgc
     7921 cgctgattaa agaaatcgtt acccatatct aattatttct tcgaattcct ctttataaga
     7981 taatcagggc tttattttca gccctggttg ttttatattc atcgctgttg cttatttaga
     8041 cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg tgttgctgcg
     8101 gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact ggcgttggta
     8161 ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc tatttcgctc
     8221 acgttttgcg cgcaagatgt tgactccaga tgccttttct cccgcaggct tttgcgttga
     8281 cgacgccgcg ctctatttct cttttgaaga aaagtgccgc gattttaatt tatctaaaga
     8341 acaaaaagcc gaactggtgc tgaatgcact ggtagcaatt cgctatctca aaccgcaaat
     8401 gccgaaaagc tggcattttg tttcccatgg tgaaatgtgg gtgcccatgc cgggcgatgc
     8461 ggcctgcgtt tggttgagcg atactcacga gcaggtcaat ttgctggtcg ttgaatctgg
     8521 cgaaaatgcc gcactatgcc tgctggcaca accctgcgtt gttattgcgg gtcgcgcgat
     8581 gcagttgggc gacgccatta aaatcatgaa cgacaggctg aaaccgcagg ttaatgttga
     8641 cagcttcagc ctcgaacagg cagtctaacg cgccaactta agtgcagtct tcggtacaca
     8701 gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg gcatgacttc
     8761 gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag aataagggat
     8821 acgaattccc tgattttcta attgctccag caacacctgt tggttatttc cacgaaatgc
     8881 ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt tggcggtatc
     8941 atcagcggca gctgcgccgt aaatttttgc cggagccgtt gccagaattt ccacctcatc
     9001 gccaacgcga atcacgccgc tattacgggc aattaaattc tgaccaaaat cgacatcgcc
     9061 gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg ccggatgttt
     9121 ttgccctttt tctgggctga cggtggtgaa aatacagcgg ctacaaggtt taaccacatc
     9181 aaacaccaca tcaccaatgc gaatcacttt ccagcgatct tcttcccagg ctgacgcgcc
     9241 ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac aacgttgttg
     9301 gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag caaatgacag
     9361 aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt ggccccaccc agcgtaattg
     9421 cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggcg caattcgcgc
     9481 ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg
     9541 aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca caggtgaagg
     9601 agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt caggttccgt
     9661 gatcataaag atgcgatcga aggccagacc actgacatca gccagagcat gtgtaagacc
     9721 aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg ccacggtgcg
     9781 gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta gctataatgc
     9841 gcaacaattt tcttagcgcc tgtcccatta ggctatttta tttgtcagtt tggccctggg
     9901 ttgtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct gtccatgtcc
     9961 agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag tgacgatatg
    10021 aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa
    10081 aacctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa gggcgacaca
    10141 cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat gttgccgctg
    10201 ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc gatcaactgg
    10261 acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt gaatgacacc
    10321 atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcacg
    10381 cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc
    10441 tggctgcata aagaaaccgc cagtatcgct cttgatctga gtggtgatgg tttacatctg
    10501 cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc agccgctatt
    10561 gtgatgcgaa ccggctggca gccaggaaca ccgctgctcg atccgatgtg tggttccggt
    10621 acgttgctga ttgaagcggc gatgctagcg accgatcgcg caccaggctt gcaccgtggg
    10681 cgttggggct ttagcggctg ggtgcagcat gatgaagcta tctggcagga agtgaaagcg
    10741 gaagcgcaaa ctcgtgcccg taaaggcctg gctgagtata gctctcattt ttacggttcg
    10801 gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc ggggattggt
    10861 gaactgatca cctttgaggt gaaagatgtc gcgcaactga ccaatccgct gccgaaaggg
    10921 ccgtacggca cagtgttgag caacccgcca tacggggaac gtctggacag cgaaccggcg
    10981 ctgattgcgc tgcatagcct gctgggtcgg atcatgaaaa accagtttgg tggctggaat
    11041 ctctctttgt ttagtgcctc gccggatctg ctaagctgct tgcagctgcg tgcagacaaa
    11101 cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga aaaattacca cgttgccgaa
    11161 agcactccag acagcaaacc ggcgatggta gcggaagact acgccaaccg cctgcgtaag
    11221 aacctcaaaa aattcgagaa gtgggctcgc caggaaggaa ttgaatgtta ccgcctgtat
    11281 gacgccgatc taccggaata taacgttgcc gttgaccgtt atgccgactg ggtggtggtg
    11341 caggagtatg cgccgccaaa aactattgat gctcacaaag cgcgtcagcg tctgttcgat
    11401 attatcgctg caaccatttc ggtactgggg attgcgccaa acaaactggt gctgaaaacc
    11461 cgtgaacgcc agaagggcaa aaaccagtac cagaaactgg gcgagaaggg cgagtttctt
    11521 gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattatct cgatactggt
    11581 ctgttcctcg atcaccgtat cgcccgccgt atgctcggtc agatgagcaa aggcaaagat
    11641 ttcctcaacc tgttctctta taccggtagc gccaccgtgc atgcgggatt aggcggcgcg
    11701 cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg agtgggcaga acgcaacctg
    11761 cgtctgaatg gcctgaccgg gcgtgcgcat cgcctgattc aggccgattg cctggcgtgg
    11821 ctgcgtgagg caaatgaaca gtttgatctg atctttatcg atccgccaac cttctctaac
    11881 tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atatggcgct gatgaaagat
    11941 ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct cgaacaacaa acgtggcttc
    12001 cgtatggatc tcgacggcct ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa
    12061 acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct gattaccgca
    12121 gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg tcgttcagcg
    12181 acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt gtttgtctgg
    12241 tgggtcgcaa cggcgcgggc aaatcgacgt taatgaaaat cctcaaccgt gaacaagggc
    12301 tggatgacgg tcgcattatt tacgagcaag atttgattgt agctcgtctg caacaggagc
    12361 cgccgcgtaa cgttgagggt agcgtttatg atttcgttgc cgaaggcatt gaagaacaag
    12421 cggaatatct gaaacgctat cacgatattt cgcgcctggt gatgaacgac ccgagcgaga
    12481 aaaatctcaa cgaactggcg aaggttcagg aacagttgga tcaccacaac ctgtggcagc
    12541 tggaaaaccg catcaacgaa gtgctggcgc aactggggtt agatcctaat gttgcgctgt
    12601 cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg gtgagtaatc
    12661 cgcgcgtgct gttgcttgat gaaccgacaa accacctgga tattgaaacc atcgactggc
    12721 tggaagggtt ccttaaaacc ttcaatggga cgattatttt catctcccac gaccgttcgt
    12781 ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctg gtgacctatc
    12841 cagggaatta cgaccagtac ctgcttgaaa aagaagaagc cctgcgcgtg gaagaactac
    12901 aaaatgccga gttcgatcgc aaactggcgc aggaagaggt gtggatccgc caggggatca
    12961 aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcaatg cgtcgcgagc
    13021 gtggtgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag gccagccgct
    13081 ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttgacggt aagcaactgg
    13141 tgaaagattt ttcggcccag gttctacgtg gcgacaaaat tgccctgatt ggtccgaatg
    13201 ggtgcggcaa aaccacgctg ctgaaactga tgctcgatca gcttcaagcg gacagcgggc
    13261 gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgc gcggaactgg
    13321 atcccgataa aacggtgatg gataaccttg ccgaaggtaa gcaagaggtg atggttaacg
    13381 gcaagccacg ccacgtattg ggctatttgc aggactttct gttccatccg aaacgggcga
    13441 tgacgccggt acgtgcgctt tctggcggtg agcggaaccg cttgctgctg gcgcgtttgt
    13501 tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt gatgtcgaaa
    13561 cgctggaact gctggaagaa ctgatcgaca gctatcaggg cacggtattg ctggttagcc
    13621 acgatcgtca gtttgtcgat aacaccgtta cagaatgttg gatcttcgaa ggcggcggta
    13681 aaattggtcg ttatgtcggc ggttatcatg atgcccgtgg tcagcaagag cagtatgtgg
    13741 cgctcaaaca gcctgcggtg aaaaaaaccg aagaagccgc cgcggcaaaa gcagaaactg
    13801 taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag cagctaccgc
    13861 aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaggtg gcggatgctt
    13921 ccttcttcag tcagccacat gagcagacac aaaaagtgct cgccgatatg gctgctgcag
    13981 agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta aaaaatggtg
    14041 gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa ttgcctgatg
    14101 aactgggttt atcaggcatg aaaaccaaca ctttgttagc catctgtaaa atagcgcatc
    14161 attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg tgctcgcagt
    14221 gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcgaca tgtccccggt
    14281 gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtccgacc gcctatgcgt
    14341 tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat atgaacgttg
    14401 caggagttac cagtgaaatt acattactgg aaattcccgg cgtgcttttt tctgaggact
    14461 acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg ttttgtctga
    14521 taaccattct gttactggtg aatcgcgcgg aattaccggt ccgtttaaaa gagcaactgg
    14581 cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc gctggtgtgc
    14641 tggtcagttt cgttaaactg atggcttacg gcagcattgg ggtaggcagc agctttctcc
    14701 cctggtgttt attttgtgtc ctgcaactgc gcgcttttca gtgcgttgat cgtcgctggt
    14761 tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacca ggcgtcacgg
    14821 ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc aatccttcct gctgatgaac
    14881 ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg tagaaacagc cagcagtgga
    14941 cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc ggctaatatt ttgcccatca
    15001 tggtgacgga tttattaggc tcgaagatgc cgtcgacgat tctcgctggg gtcattctgt
    15061 tatggagcga aggctcttat cccgtcgctg cggtgatctt tctggccagt attatggtgc
    15121 caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga tgccaaaggg catggcaagc
    15181 gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc cgctggtcga
    15241 tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga ggtttaatga
    15301 gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg acaatgtttt
    15361 ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca gagcatgagg
    15421 agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt gaagaactgg
    15481 tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt tcttttttat
    15541 cattacagcc atcagggact ggaagtgacc ctgatcaccg cgaatgcgga aggaattgaa
    15601 ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga aagcgccaca
    15661 ctggctgatg atttgacgca cgttgaaatc aaagtgcggc tgaattctgg tatggaaaaa
    15721 ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc agattggtcg cgaagggatt
    15781 agcggcctgg gaacgctgct gtctggagtt tatatcgaac tgcagccagg cgcgaaaggc
    15841 agcaaaatgg ataaatacga tttgctggac tcgccaccgt tggccccgcc tgatgcgaaa
    15901 ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg agatccggtg
    15961 ctgttccgtg gctatcgggt aggttcggtt gaaaccagca ccttcgatac gcaaaaacgc
    16021 aatatcagtt atcaactgtt catcaatgca ccttatgacc gactggtgac cagcaatgtt
    16081 cgcttctgga aagatagtgg cattgcggtt gatctgacgt cagcggggat gcgtgtggag
    16141 atgggctcat tgacaacgct gcttagtggc ggtgttagct ttgatgtgcc ggaaggtctg
    16201 gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga tcagaagagc
    16261 attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa agattcggta
    16321 cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg taccgtaagc
    16381 aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta ccgtattccg
    16441 gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa tgcggatgtt
    16501 gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa aaccggaaac
    16561 ctggtcactg gtgcactgta tgttgatctt gatttctatc caaatacgcc tgcaataacc
    16621 ggtattcgtg aatttaatgg ttatcagatt atcccgactg ttagcggcgg cctggcgcaa
    16681 atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt gaatccgatg
    16741 attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa cctgcaaacg
    16801 acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagtt gccgacggat
    16861 atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc tggctccgca
    16921 gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg agaactgcaa
    16981 ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc gaaggacaaa
    17041 aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg attgcagcac
    17101 tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag ttacctgtgg
    17161 tgcagagcgg tacacaaagt accgccagcc agggcaatcg tctgttatgg gtagagcagg
    17221 tcactgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt gatgtgaagt
    17281 atgtgattgc caacaacaac ttgtgggcca gcccgttgga tcaacaattg cgcaacaccc
    17341 tggttgccaa cctgagtacg caactgcccg gctgggtggt tgcctcccag cctctgggaa
    17401 gcgcccagga cacgctcaat gttaccgtaa cggagtttaa cggtcgctat gatggcaagg
    17461 tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa cgtccgttcc
    17521 gtctggaagg agtgcaaact caggatggtt acgatgagat ggttaaagta ctggccagtg
    17581 tctggagtca ggaagccgct tctattgcgc aagagataaa gcgtctacct taattataaa
    17641 gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg tttctaacgt
    17701 gcggaaaaag ttgttcctct ttacattttt tgtacaaccg acatacccgt gtagctcaca
    17761 aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg gtattgctta
    17821 actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca gtatgaggga
    17881 aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa cgtggttatc
    17941 aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg aatcaaaggt
    18001 cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg gcctgattct
    18061 gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac tctgtctgat
    18121 tataatcaga aggcagaagt atcctggaac agaccgactt tcaggtcgct ggcggtatag
    18181 atcaaacgac catcaaccag cacttctcca tccgccaggc ccataatcag acgacggtta
    18241 acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag tacctgacca
    18301 gtgaatttca cttcgccaac gcccagtgcg cggcctttac cttcgccgcc cagccagccg
    18361 aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg cataaccgga
    18421 tcgccaataa agtggcatcc gaagaaccac agatccggat tgatatccag ttctgcttca
    18481 acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg gtccatcatc
    18541 agcatattcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc gcgaccagag
    18601 gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc tgtaagcctt
    18661 attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc gatcagttcg
    18721 gaataaacca gttcagccaa cgtaatggcc atggaaaacg gtgacgtcct tcctgttgcg
    18781 atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct tcgccatccc
    18841 acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc cagatggtga
    18901 atttgccttc ttctaccgct ttcactagtt cactgtgaag acttaaatgg cgaacgttag
    18961 ctgtggggat aatgacacct tgtttcccgg ttaactcacg ttgctggcaa atagcaaaga
    19021 agccttcgat tttctcattt aaaccaccga ccggctgggc gcgaccgaac tgatcgactg
    19081 aacctgtgat agcgatactc tgattcaccg gcacatcggc gagggcgctt atcagggcgc
    19141 agagttcagc catcgaggca ctatctccat caacttcact gtatgactgc tcaaatgtca
    19201 gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg aacgcttgca
    19261 tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt tcgatgtcgg
    19321 tgaattcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg ccaaaagcgc
    19381 gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg cgttcgcctt
    19441 cggtttcaat caggatttgc tcctgaagga tctcatcctg catacgttca gcgaggaaac
    19501 cttcgcgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag aaggtgtcgc
    19561 catcacacag ggaggcgacc tctttacact ggcggaggat ccactgcggg ctaagcggaa
    19621 gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat aagtaccggc caggcatccg
    19681 ctcccggtgc aggtaagtga ttatgtctgg cggtaaatgt cacccagcga caccactggg
    19741 ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa atagcctgct
    19801 ctgaaagctc tggctccatc tcctggaaat cagccaatga ttcgcgttcg cctaccagaa
    19861 tgactttcag cttcaatggc atcgaaggca cagagacggg gagagggcgc gactcatcaa
    19921 acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc cacagcagag
    19981 gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc tgatgcacca
    20041 gaccaggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag agttgctccg
    20101 cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca tctgcactca
    20161 ctgcgtgacg taagcggatg gagtggccgg aaacctcata gtgaccgccc accagttggc
    20221 ctgcatcgct ttgtagcgta cgcgcggcat tggcaataag attcagatac tcagactctt
    20281 ccggggcctt cgccagcata aaagaggagg atgctcgcgt atgcagcaac tgctccagcg
    20341 caaattgcag ccgcggttga gtatcactga ataaaggatc gttttcgtca atcaaatgtg
    20401 gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc catgcaagtt
    20461 tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt ataccgtaac
    20521 cggcactaca gcacacgtag aaagcaccga caatactcca ggcatgggcg ttaaagctca
    20581 caggatggag attcttttct tcactggcct aaaaagctga tattctgtaa agagttacac
    20641 ggtaacattg agatcgctat gaaatatcaa caacttgaaa atcttgaaag cggttggaaa
    20701 tggaagtatc tggtgaagaa gcatcgcgaa ggggagttaa tcacccgtta catagaagcc
    20761 agtgccgccc aggaagccgt cgatgtgttg ctctcgctgg aaaatgaacc cgtgctggta
    20821 aatggctgga ttgacaagca tatgaatccg gaactggtca atcgcatgaa gcaaaccatt
    20881 cgggcaagac gaaagcgcca ttttaatgcg gaacaccagc atacgcgcaa aaaatcgatc
    20941 gatctggaat ttattgtctg gcaacgtctg gctggtcttg cgcagcgtcg cggtaaaacg
    21001 ctttctgaaa cgattgttca gctgattgaa gatgcggaaa acaaagagaa atacgcgaat
    21061 aaaatgtctt ctctgaagca ggatctgcag gcattgctgg gtaaggaata actgacgaaa
    21121 gtcagttcaa tttactaaag gcaaaaaaaa ccccgcagca gcggggtttt tctaccagac
    21181 gagaacttaa gcctgcggct gagttacaac gtctttgata cctttaactt cgatctctac
    21241 gcgacgatcc ggagccaggc agtcgatcag tgcagcacgc tgtttcacgt tgtcacaggt
    21301 gttgccagta accgggttgg attcgcccat accacgtgcg gagatcttgt ctgccgggat
    21361 acctttggag atcaggtaat caacaacaga ctgagcacgg cgctcggaca gaccctggtt
    21421 gtaagcgtca gaaccgatgc ggtcggtgta acccagaaca actacggaac cgtctttcgg
    21481 atccaggttg ctcagctggc tgtacagctg atccagagca gcctgacctt ccggtttcag
    21541 ggttgctttg ttgaagttga acagaacgtc agacttcaga gtgaagtgct tggtctgtac
    21601 ttccggtgcc ggagctggag ccggagcaac tactggagct gcttcgccct gaccgaaacg
    21661 gtaggaaacc cccaggctca gcatgccgtt gtccggacga gtgccgatgg tgtgtgcgtc
    21721 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    21781 ctcaacaccg ccagcgaaga ccggagaaac gccggtgtcg tggtttttac cataaacgtt
    21841 ggatttagtg tctgcacgcc ataccatacc acccagacga gtgtagatgt ccaggtcgtc
    21901 agtgattggg taacccagtt tagcggtcag ttgaacgccc tgagctttgt atgcaccgtt
    21961 ttcaacgctg cctttgtacg gcatacgacc taaccagtcg taacccattt caaagccaac
    22021 atacgggtta acctggtaac caccaaaagc accagcgccc agttggtttt catgggtcgg
    22081 gccattgttg ttgatgaaac cagtgtcatg gtactgggac cagcccagtt tagcaccagt
    22141 gtaccaggtg ttatctttcg gagcggcctg cgctacggta gcgaaaccag ccagtgccac
    22201 tgcaatcgcg atagctgtct ttttcatttt ttgcgcctcg ttatcatcca aaatacgcca
    22261 tgaatatctc caacgagata acacggttaa atccttcacc gggggatctg ctcaattaac
    22321 tctaccgata tcttcggctt atgccgagca cccctggcga tgtaaagtct acaacgtagt
    22381 tgaaaactta caagtgtgaa ctccgtcagg catatgaaaa aaaagtcttg tataaggtat
    22441 gtttaatctt ttttgtcagc gacaatttac agaagaaaat cgcggaaacc gcttcagaca
    22501 agcctccgca aggaaaatta gtcacgactg aaagcattgg ctgggcgaca aaaaaagttc
    22561 caggattaat cctaaattta cttaatgata caaattagag tgaattttta gcccggaaag
    22621 ttgtctcgtg gcgtgagagg atgcgcttac cggacgcata ataaacccca tagcgttacc
    22681 ttcatttgcc gcatcaacaa gttcagcatg ctcttcttca gtcaaatcat ctgccaacca
    22741 accgatcacc acactgtaat tgcccgtgcg taaagcgcga accattgact ccacagtgtg
    22801 gcaaggggag agctggctaa tctgcattac tttcgttaag ggtagcccag atgcctgaac
    22861 ccattcccga ctcagttttt gttgcggtgt taaccagagt tgccagcgcg attgctgacc
    22921 gagttgctgt aacaatggca acagtagaag ttgcgtcatc atgggctgat cttcgcgata
    22981 gacaacttca ctgataagcc cggctgtagt gttttccgta gagacacgcg caattttact
    23041 tgctgcggat gagaacgacg aagaacgatg tgcatagcct gaagtgtaca taatcaatcc
    23101 agcccctatg agttactgta tggatgtaca gtacatccag tgacaacaaa gatcaaccct
    23161 attttcggaa agagcctcgc aaattttgtc gttggtgacg ggaaaacata aattaatctt
    23221 gccccttaag aataagttgc ctattttcgt agttaacgga tccgttaatg tgaatcattc
    23281 ttttatgtta tgattttaaa aggaatttta tgaaaagcct ctcctataag cggatctata
    23341 aatcacaaga atacctggca acgttgggca caattgaata ccgatcattg tttggcagtt
    23401 acagcctgac cgttgacgac acggtgtttg cgatggtttc tgatggtgag ttgtatcttc
    23461 gggcttgtga gcaaagtgca cagtactgtg taaaacatcc gcctgtctgg ctgacatata
    23521 aaaagtgtgg ccgatccgtt accctcaatt actatcgggt tgatgaaagt ctatggcgaa
    23581 atcaactgaa gctggtgcgt ctgtcgaaat attctctcga tgcagcgctg aaagagaaaa
    23641 gcacgcgcaa tacccgggaa agactgaaag atttgcccaa tatgtctttt catctggaag
    23701 cgattctcgg ggaggtgggg attaaggatg tacgggcgtt acgtatactt ggggcaaaaa
    23761 tgtgttggtt gcgactgcgg cagcaaaaca gtctggtgac agaaaagatt ctgtttatgc
    23821 ttgaaggtgc cattatcggc attcatgaag ctgcgctccc ggtggcacgc cgccaggagc
    23881 ttgcagaatg ggctgactct cttacgccga aacaggagtt tcctgcggaa cttgagtaat
    23941 ctggcgttgc agacgaccaa tctcaggcag taatgcaatc aataatccaa cttgttgtac
    24001 gaccagaggt tctttgctgt ctgcccgtgg ttcaagttgc tgcatccgct gtttcaggct
    24061 agctaatgcc tcattgacgc gttcttcatc agcaggttga tgatgtaacg cgtcatcaac
    24121 atagcaaact gcgtcatcaa gaaacgccag aatttcagga ttagttaact gctcccggtg
    24181 agcaccgagg gctgagatat agctggtaaa cgtatggtta aggcacagca accgaaacgc
    24241 ggcttcgcga atttgcgggg taacgttcgg ctcgctggac atatttgata ccaccgacgc
    24301 cagctcagca tcacggttgt gtgcatcgcg gcgggcaata cgatacgcca gacggttatc
    24361 acgcccctga tggtattgct ccagtatggc atcgagatac cgacagttgg cctctgtggc
    24421 gcgttcgagc atgcgcggca gattgcgaaa ctgccagtca ggccagatgt agctcactgc
    24481 cgcccacgca atggcacaac caatcagcgt atcgattacg cgaggtaacg ctacttcaaa
    24541 accttcaccc agtaagttaa aacacagtag caccaaaagt gtgatgaaca tcgttgcatg
    24601 agcgtattgc acgttacgga aggcaaaaaa gagcacgccg gtaataacca gcagcaccag
    24661 ctgcccttcc agtgatggca caaaccacag cacaggaatg ccaatggcga tacctaccag
    24721 cgtaccaata atccttaact tcaggcggtg gcgcgtggcg ttatagtttg gctggcagac
    24781 aaacaaactt gtcagcaaga tccaataccc gtgatgcatt ccggttatct gaatgatggc
    24841 gtagccgaag cacaatacca gcgacattct taccgcatga cggaagaggg cggattccgg
    24901 cgtgaagtga cggctaagac gcagccagat atcactcaac ccgtgcgggc tgtcatcagc
    24961 gagctcattt tcgtcattat tatggggtag tgcctgggcc tgttctgatt caattgttgc
    25021 cagttgggca tcaatggcgc gtaaattgtt cagcaaaaat cccagtgttt tgagtaaatc
    25081 ggcgggtgcg ccgttatcgc gcatccgctc cagcgcagca tcaatatgcg taaaagcgcg
    25141 ctcaaaatgc ggatcatgtt gataaggctg acgcaacaaa atacagcgtg acagttgctg
    25201 gcacgcctgg ccctgcatcg acatcagccg ctgaaaacgg aacagcacgt cgctgtggcg
    25261 aaaatgttca cgcaatgttt gatactgaat atgagaagag ctggcacgct cgtgaatatc
    25321 ctgtgcgaca aagtaataat gcagcgtgcg acgcgttccc cgttgaccac gatcgccacg
    25381 taagcgggtc agcagcgaga gtttcgtctg attcaatgtc gccatcagca gaccgttggc
    25441 gagagccaaa tcgtacagcg gtgcctggct ttcatcttca atatcaggat caaacatgcg
    25501 cgacttgagc tcaagataac gcgccagttg ttcatagcaa cgcgccaggt tgtcctgcag
    25561 cgggcggacc gggaacagca gatgaccaat aagtgtcagg acgttgtacc agacggcacc
    25621 ggccagcaga tacatcggct gctgatacca gtgctcatac agtgatgttc ccaacatagt
    25681 gtaaatggcg atcagcaatg caccgaaggc aattgttgca tagcgttgac ccagaccgcc
    25741 gagcaaaatg aagccgctgg tagagagcgt taagccaatc gcaaatagcc agggccaggg
    25801 aaacagcaat tctactgagg ccgaggcgat aaaaaagcag aacagcgtaa tgatgaggtt
    25861 acgcaaacgt cccgccagtc ggtcatcgag atcggtcagc gccgctgcca ccatccccag
    25921 cgttagcgga atcgtcagtt ttacatcacc cagccaccac ggaaacgctg tggttccaca
    25981 aagcgcaata aaaatacgcg cgtaatacag ccaggcgctg ttccaggtat agcgtttgag
    26041 caaaggactt agcataaagg ccatattagt ggattattca aaacgacgac gtgcattggc
    26101 ttcacgcgca gcttgcgctg tttctaccga taccacgcga cgaccaaccg gccatagtgc
    26161 aatagcggca attttaaagt tcgcaatgcc gacaggaatg ccaatgattg aaatacattg
    26221 tgcgatgccc gttgcaatgt gcatcaggca taaccaccag ccaaagaaaa tcagccagaa
    26281 aatattcaat accgtaccgc cagtattcag cagcacattt ttgccagccg ggttcagttc
    26341 atcgacatga atagcttcat tgccataagg caccagagat agtttagtga tctcccagca
    26401 ggatcgtgtc agcggtaagg taaaaatcag cacaatactg accagagtcg ccaacagcca
    26461 gcccagagtg gtggcaaatc cgccaagcac aaagttcaga atgttcaaaa cggtacgcat
    26521 aaaacctcgc tttactgtgg ttttcagtaa tgggcggcaa ttgtaacgtt tttttgggct
    26581 ggagcacgtt ttctctgacg gttacactga taagaaataa tttcgtgtgg atctacagag
    26641 tcatggaact gaaagcgaca acgcttggaa aacgtctggc acagcaccct tacgatcggg
    26701 cggtgatcct caatgccggg attaaagtct ccggcgatcg ccacgaatac cttattcctt
    26761 tcaatcaatt actggcgatt cactgtaagc gcggtctggt atggggcgag ctggaatttg
    26821 tactgccgga cgaaaaagtg gtgcgtctgc acggcaccga atggggcgag acgcagcgtt
    26881 tttaccatca tcttgatgct cactggcggc ggtggagtgg cgagatgagc gaaattgcgt
    26941 ctggtgtttt acgccagcaa ctggatttga ttgccacgcg cactggagaa aataaatggc
    27001 tgacgcgtga gcaaacctct ggcgttcagc aacaaatccg ccaggctttg tcggcgttgc
    27061 cgttgccggt taaccgactg gaagaattcg ataactgccg tgaggcgtgg cgtaaatgtc
    27121 aggcctggtt gaaagatatt gaaagcgctc ggttgcagca taaccaggcg tataccgaag
    27181 ccatgcttac cgagtatgcg gattttttcc gccaggtcga gtcttcaccg ctgaatccgg
    27241 cgcaggcccg ggcagtcgtt aatggcgagc attctctgtt agtgctggca ggtgcaggaa
    27301 gcggaaaaac gtcggtgctg gtggcccgtg caggctggtt gctggcgcgt ggtgaagcgt
    27361 cccctgagca aattttattg ctggcgtttg gtcgcaaagc cgctgaagag atggacgagc
    27421 ggattcgcga acggctacat accgaagaca ttaccgcacg cacgtttcat gcgctggcgc
    27481 tgcatattat tcagcagggc agcaaaaaag ttccgatagt cagcaaactg gaaaatgata
    27541 ccgctgcccg tcatgaactc tttattgctg agtggcgcaa gcaatgcagc gaaaagaaag
    27601 cgcaggcgaa gggctggcgg caatggctga cggaagaaat gcagtggtca gtgccagaag
    27661 gtaacttctg ggatgatgaa aaattacagc gtcgccttgc ctctcgcctc gatcgctggg
    27721 taagtctgat gcggatgcac ggtggtgcac aggcagaaat gattgccagt gcacccgaag
    27781 agattcgcga tctgttcagt aaacgtatca agttgatggc cccgttatta aaagcctgga
    27841 aaggtgcgct gaaggcagaa aacgctgtcg atttttcggg ccttattcat caggcgattg
    27901 tgattctgga gaaaggtcgc tttatcagcc cgtggaagca tattctggtt gatgaatttc
    27961 aggatatctc gccgcagcgg gcagcgttgt tagcggcatt acgcaagcaa aacagtcaga
    28021 cgacgttgtt cgctgttggt gatgactggc aggcgattta ccgattcagc ggtgcgcaaa
    28081 tgtcgctcac caccgctttc catgaaaact ttggtgaagg cgaacgttgt gatttagaca
    28141 cgacttaccg ttttaacagt cgtatcggta aggtggcaaa ccggtttatt cagcagaacc
    28201 caggccagct gaaaaagccg ctaaacagct taaccaatgg agacaaaaaa gccgtcacgt
    28261 tattggatga gagtcaactt gacgctttgc tggataagct ctctggttat gccaaaccgg
    28321 aagagcgcat tctgatcctg gcgcgttacc atcacatgag gcctgccagc ctggaaaaag
    28381 cggcaacacg ctggccgaag ttgcaaatcg actttatgac cattcatgcc agcaaagggc
    28441 aacaggcgga ttacgtcatc atcgttggct tgcaggaggg aagtgatggt tttccggctg
    28501 cggcgcggga gtcgattatg gaagaggcgc tactgccacc ggttgaggat ttcccggacg
    28561 ctgaagaacg gcggttaatg tacgtggcgc tgacccgggc acgccatcgg gtatgggcac
    28621 tgtttaacaa agagaatccc tctccctttg tggaaatact gaaaaatctg gatgtgccgg
    28681 tggcgagaaa accgtaagaa acaggtggcg tttgccacct gtgcaatatt acttcagacg
    28741 gtccgcgaga taacgctgat aatcggggat tagaatatcg accgcgtcgt tgaaatgcgg
    28801 cgactggatt atgaagtctg ccgttgccac gttggtggcg accggaatgt tccataccgt
    28861 cgccagacgc agcaaggctt tcacgtcagg atcgtgcggc acggcattta gtggatccca
    28921 gaagaaaatc aatacatcaa ttttcccttc tgagatcaat gcgccaacct gctggtcacc
    28981 ccccattggg ccactcaaca tcgcgttgac gttcatgccg gtcgcgcggg aaattaagtt
    29041 accggtagtg cctgttgcat acagtacgtg ttgttccagt aacggttgat gccgttccac
    29101 ccagctcatc agcatttgtt tgcagtgatc gtgtgccacc agcgcaatat gtttccgcgc
    29161 aggtaaagtg cgagtcgtca gttccataat gtacatccgt agttaacttt cctacagatt
    29221 actgtaagca cttatcgctg caagataaag accgaaaaag cctgcgcaca ggcacaaaaa
    29281 tctcaggaag atggttgttt ttccgcccac tgcaggaaag tatttcgcgt ttgtgggtca
    29341 gccagtttaa accaatactt cagccgttgt tctgtgagca cctgagactg cggtggaata
    29401 gcatccagct cggaaacacc cgaaagcagc gtactgtcat ctgccatcat cgtggcaaat
    29461 tgcggtagtg aagcgcgttt cgcggatttg ttatagcgtt caacctcgac ctcataatca
    29521 atcgtttttg ccgttgaggt aatggcgaga atatccagct ttaccggaat cggcgtcgca
    29581 tcgccatcca acaattcaag gcgcggcgcg gcatcaaaat ggttagcttc ccgctcattc
    29641 tccaggcgag gcaggcgaaa attgacctgg ttgatgagct gggtattaaa actgaccacc
    29701 agcggtgggg agatatacag ccgttcttca ctattggaaa gatgaatcgt cttctcaacg
    29761 cgaaacacta actgatgcgg tccattatcc agttcaatgc tgtccgcacc gcgcagcaga
    29821 gagctggaaa ccttcttgcc gtcgagtacc agaagatcga catcggttga aagccgcagc
    29881 gtggtggcaa aaacggatac cggcaaacat aatgcaatta aggtggtcac gatgccggtt
    29941 ttcataggta gctcctgtca aaagaccgcc cgaataataa tgtgtcaaaa tttttcttta
    30001 atca
//
LOCUS       DAJTYZ010000040        29549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000040 DAJTYZ010000000
VERSION     DAJTYZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.040"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..690
                     /gene="dgt"
                     /locus_tag="OO596_001721"
     CDS             <1..690
                     /gene="dgt"
                     /locus_tag="OO596_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCR6717592.1"
                     /translation="LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSR
                     SNSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDL
                     LKLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKR
                     FPIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAW
                     DEYRRLMAVEQ"
     gene            820..2244
                     /gene="degP"
                     /locus_tag="OO596_001722"
     CDS             820..2244
                     /gene="degP"
                     /locus_tag="OO596_001722"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6717593.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2399..3556
                     /gene="cdaR"
                     /locus_tag="OO596_001723"
     CDS             2399..3556
                     /gene="cdaR"
                     /locus_tag="OO596_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR6717594.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3645..4031)
                     /locus_tag="OO596_001724"
     CDS             complement(3645..4031)
                     /locus_tag="OO596_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6717595.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4193..5005)
                     /gene="yaeI"
                     /locus_tag="OO596_001725"
     CDS             complement(4193..5005)
                     /gene="yaeI"
                     /locus_tag="OO596_001725"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR6717596.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5060..5884)
                     /gene="dapD"
                     /locus_tag="OO596_001726"
     CDS             complement(5060..5884)
                     /gene="dapD"
                     /locus_tag="OO596_001726"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6717597.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5915..8587)
                     /gene="glnD"
                     /locus_tag="OO596_001727"
     CDS             complement(5915..8587)
                     /gene="glnD"
                     /locus_tag="OO596_001727"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6717598.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8649..9443)
                     /gene="map"
                     /locus_tag="OO596_001728"
     CDS             complement(8649..9443)
                     /gene="map"
                     /locus_tag="OO596_001728"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6717599.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9811..10536
                     /gene="rpsB"
                     /locus_tag="OO596_001729"
     CDS             9811..10536
                     /gene="rpsB"
                     /locus_tag="OO596_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6717600.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10794..11645
                     /gene="tsf"
                     /locus_tag="OO596_001730"
     CDS             10794..11645
                     /gene="tsf"
                     /locus_tag="OO596_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6717601.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11792..12517
                     /gene="pyrH"
                     /locus_tag="OO596_001731"
     CDS             11792..12517
                     /gene="pyrH"
                     /locus_tag="OO596_001731"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6717602.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12809..13366
                     /gene="frr"
                     /locus_tag="OO596_001732"
     CDS             12809..13366
                     /gene="frr"
                     /locus_tag="OO596_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6717603.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13458..14654
                     /gene="ispC"
                     /locus_tag="OO596_001733"
     CDS             13458..14654
                     /gene="ispC"
                     /locus_tag="OO596_001733"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6717604.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14843..15601
                     /gene="ispU"
                     /locus_tag="OO596_001734"
                     /gene_synonym="uppS"
     CDS             14843..15601
                     /gene="ispU"
                     /locus_tag="OO596_001734"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6717605.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15614..16471
                     /gene="cdsA"
                     /locus_tag="OO596_001735"
     CDS             15614..16471
                     /gene="cdsA"
                     /locus_tag="OO596_001735"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6717606.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16483..17835
                     /gene="rseP"
                     /locus_tag="OO596_001736"
     CDS             16483..17835
                     /gene="rseP"
                     /locus_tag="OO596_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6717607.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17865..20297
                     /gene="bamA"
                     /locus_tag="OO596_001737"
     CDS             17865..20297
                     /gene="bamA"
                     /locus_tag="OO596_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6717608.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20419..20904
                     /gene="skp"
                     /locus_tag="OO596_001738"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20419..20904
                     /gene="skp"
                     /locus_tag="OO596_001738"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6717609.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20908..21933
                     /gene="lpxD"
                     /locus_tag="OO596_001739"
     CDS             20908..21933
                     /gene="lpxD"
                     /locus_tag="OO596_001739"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6717610.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22038..22493
                     /gene="fabZ"
                     /locus_tag="OO596_001740"
     CDS             22038..22493
                     /gene="fabZ"
                     /locus_tag="OO596_001740"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6717611.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22497..23285
                     /gene="lpxA"
                     /locus_tag="OO596_001741"
     CDS             22497..23285
                     /gene="lpxA"
                     /locus_tag="OO596_001741"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6717612.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23285..24433
                     /gene="lpxB"
                     /locus_tag="OO596_001742"
     CDS             23285..24433
                     /gene="lpxB"
                     /locus_tag="OO596_001742"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6717613.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24430..25026
                     /gene="rnhB"
                     /locus_tag="OO596_001743"
     CDS             24430..25026
                     /gene="rnhB"
                     /locus_tag="OO596_001743"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6717614.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25063..28545
                     /gene="dnaE"
                     /locus_tag="OO596_001744"
     CDS             25063..28545
                     /gene="dnaE"
                     /locus_tag="OO596_001744"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6717615.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEDMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28558..29517
                     /gene="accA"
                     /locus_tag="OO596_001745"
     CDS             28558..29517
                     /gene="accA"
                     /locus_tag="OO596_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6717616.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7121 c         8159 g         7110 t
ORIGIN      
        1 cttgaagatg cggtagagaa aagaatattt accgttgagc agctttatca tcatttgcac
       61 gaggcgtggg gtcagcatga gaaaggttcg ctcttttcgc tagtggttga aaatgcctgg
      121 gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt tatgtattta
      181 cgggtaaaca ccctaaataa actggtaccc tacgcggcac aacgatttat tgataatctg
      241 cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag cgaatgcagc
      301 gatcttctta agctatataa aaatgtcgct gtaaaacatg tgtttagcca tccggatgtc
      361 gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat ttatcgtcct
      421 ttattaagcc tgtcgttatc agactttact gaactggtag aaaaagaacg ggtgaaacgt
      481 ttccctattg agtcgcgctt attccacaaa ctctcgacgc gccatcggct ggcctatgtc
      541 gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga atattattac
      601 cgttgccgcc tactgcagga ttatatcagc ggtatgaccg acctctatgc gtgggatgaa
      661 taccgacgtc tgatggccgt agaacaataa ccaggctttt gtaaagacga acaataaatt
      721 tttacctttt gcagaaactt tagttcggaa cttcgcgcta taaaacgaat ctgaagaaca
      781 cagcaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac cacattagca
      841 ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc aacggcggct
      901 gagacttctt cagcaacgac agcccagcag atgccaagcc ttgcaccgat gctcgaaaag
      961 gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa tacgccgcgt
     1021 atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca ggaaggttca
     1081 ccgtttcaga gctctccgtt ctgccagggg ggccagggcg gtaatggcgg cggccagcaa
     1141 cagaaattca tggcgctggg ttccggcgtc atcattgatg ccgataaagg ctatgtcgtc
     1201 accaacaacc atgttgttga taacgcgacg gtgattaaag tccagctgag cgatggtcgt
     1261 aagttcgacg cgaagatggt tggcaaagat ccgcgctctg atatcgcgct gatccaaatc
     1321 cagaacccga aaaacctgac cgcaattaag atggcggatt ctgatgcact gcgcgtgggt
     1381 gattacaccg tagcgattgg taacccgttt ggtctgggcg agacggtaac ttccgggatt
     1441 gtctctgcgc tggggcgtag cggcctgaat gctgaaaact acgaaaactt catccagacc
     1501 gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg ttaacctgaa cggcgaactg
     1561 atcggtatca acaccgcgat cctcgcaccg gacggcggca acatcggtat cggttttgct
     1621 atcccgagta acatggtgaa aaacctgacc tcgcagatgg tggaatacgg ccaggtgaaa
     1681 cgcggtgagc tggggattat ggggactgag ctgaactccg aactggccaa agcgatgaaa
     1741 gttgacgccc agcgcggtgc tttcgtaagc caggttctgc ctaattcctc cgctgcaaaa
     1801 gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccgat cagcagcttt
     1861 gccgcactgc gtgctcaggt gggtactatg ccggtgggca gcaaactgac cctgggctta
     1921 ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc agcagagcag ccagaatcag
     1981 gttgattcca gctccatctt caacggcatt gaaggcgctg agatgagcaa caaaggcaaa
     2041 gatcagggcg tggtagtgaa caacgtgaaa acgggcactc cggctgcgca gatcggcctg
     2101 aagaaaggtg atgtgattat tggcgcgaac cagcaggcag tgaaaaacat cgctgaactg
     2161 cgtaaagtgc tcgacagcaa accgtctgtg ctggcactga acattcagcg cggcgacagc
     2221 accatctacc tgttaatgca gtaatctccc tcaacccctt cctgaatacg ggaaggggtt
     2281 ctccttacaa tctgtgaact tcgccacaac tccatacatc ttcatcatcc tttgggcatt
     2341 tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataacg gaggacttat
     2401 ggctggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta ccatgcgcat
     2461 catcgatacc aatatcaacg taatggatgc ccgcgggcga attatcggca gcggcgatcg
     2521 tgagcgtatt ggtgaattgc acgaaggtgc attgctggta ctttcacagg gacgagtcgt
     2581 cgatatcgat gacgcggtag cacgtcatct gcacggtgtg cggcagggga ttaatctacc
     2641 gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac cagagaatct
     2701 gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac agtcgcggtt
     2761 gatgcacctg ttggcgcagg atagccgttt gcgggaagaa ctggtgatga acctgattca
     2821 ggctgaggag aatactcccg cacttactga atgggcgcaa cggctgggga ttgatctcaa
     2881 tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggcg tggacagcgc
     2941 aatggcggag ttacaacaac tgcaaaatgc gctgactacg cccgagcgta ataatctggt
     3001 ggcaattgtt tcgctgaccg aaatggtggt gttgaaaccg gcgttgaact cttttgggcg
     3061 ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca tgaaagagta
     3121 cggccagctg cgttttcgcg tttcactggg caactatttt accggtcctg gcagtattgc
     3181 ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga tgccagaaag
     3241 tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga
     3301 ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg acaataatgg
     3361 cttgctgcga cgaacgctgg cggcgtggtt tcgccacaat gtgcaaccgc tggctacgtc
     3421 aaaggcgctg tttattcatc gtaatactct ggagtatcgg cttaatcgta tatcggaact
     3481 gaccgggctt gatttgggta attttgatga caggttgctg ctgtatgtgg cgttacaact
     3541 ggatgaagag cggtaggtta tgcgttaagg tcgaggcggc gctggctcat tcccctcagc
     3601 ccgaagggta gaggggaatg tccgagttaa ccgtcagttc ggcattattt acgcgttagt
     3661 ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc caggtgacgt
     3721 aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg gcagatttga
     3781 tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc tttataaccc
     3841 tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa cttcacgctc
     3901 ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc gttgttggct
     3961 tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag acttttcaga
     4021 ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt tagcatttgg
     4081 catggcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga cttctatcat
     4141 ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggtgat aaaattcacg ttctacacca
     4201 gttccagcat cgttacttcc gggcggcagt tcagacgcaa accatacaaa ctgcccacgc
     4261 cacgggttgt gtagatgtgt cgttcgccaa aggcatttaa tccggcgacg taacgtttat
     4321 cttcgacagg ggcaaaaggt tcaccaacca acggtacgcg cagttgcccg ccgtgagtat
     4381 ggccgcacag catcagatcc cagggttcat cacgcatgac ttctttgctg tcgggattat
     4441 gtgccagcat cagtcttggc agatttgctt cgctggcggg aggtggtttg cattgtccgg
     4501 cccataagtc accggtgcct accagttcga attgcctgtt cggcgtggcg atcaccgtag
     4561 cctggttaaa caacaccgtg atgcccactg atttcaacgt ctcgccaatt aagtgatttt
     4621 tttctgtacc aacagggcga tcgtggttgc cgaagcaggc aaacgtcggc gcacattcgg
     4681 caagggggga gaggacatca ctaaacgccg aaaaattcag cggcatatca aataatacgt
     4741 aatcgccgcc cagtaatatc aaatcgggct tttgttctat gccaagagca atcgcgtcag
     4801 aaatcaggct taaaggaaca aaacgagagt aatggagatc ggccagaaaa agaattttga
     4861 atggtgctgc gttgtcttta aaaaaggcga ggcggtgacg gattaattca aaccagccta
     4921 gctcccagta atgcatataa ccgaaacctg agctcgtggc tatcgtcgcg gcagtagcct
     4981 gcaacaatcg gcggcgtgaa atcatcgctc atccctgcaa aaaaaatcgg gcagcgtcgt
     5041 gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag ttcgttaatg ccgactttgc
     5101 cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg tatttgccat
     5161 cttttgacgg cagattacct gaaacaacca cagaccccgc cggaacgcga ccgtagtgga
     5221 tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg cccatggaaa
     5281 tgacggaacc ttcttcgaca atcacccctt caaccacttc agagcgtgcg ccaatgaagc
     5341 agttatcttc aatgatggtt gggttagcct gcagcggttc cagcacgccg ccgatgccca
     5401 cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg gtcgcccagg
     5461 tatcaaccat ggtgccttca tcaacatatg cgccgatgtt gacgtaagac ggcatcagca
     5521 cggtgttacg ggcaataaac gcaccctgac gtaccgccgc tggtggcaca acgcggaagc
     5581 cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt catcggcact ttgtcgaagt
     5641 agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag agcagcaccg
     5701 cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct acacgcagtg
     5761 caccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg gtgtctgcgt
     5821 tggctggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc tgtaactgct
     5881 gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt ggattgaggg
     5941 cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagtgca cgccggtcag
     6001 cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt cgggcaccat
     6061 gaagcgaaat tcccagatcg gcaaaaattt tcccgactct cgccagcagt cctggctggt
     6121 cgagcgcgat aagctcaagg aacgatttgc ggtcggtatg ggttggcaaa aacgtcactt
     6181 cggtttcaac agtaaagtga cgtagtttag ccggttgacg acgcggctgt ggtggctgcc
     6241 agctactttg tgtcagtacc tgctccagac caaaccgaat aacctcatga cgatctgcgg
     6301 atagcgggct accatcgggt tccagcacga tgaaggtatc catcgccata ccgtcgcgag
     6361 tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg gcacagacgg
     6421 cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct ccacgcgtag
     6481 cctgcgggct aagcaatacc agcggtttgc ttaaatcatg ctgtaataaa tggcgggcat
     6541 gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag cgtgaccaaa
     6601 tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt gcctggagtt
     6661 ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt cgtagctgct
     6721 tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc cacagcgttt
     6781 cgttggtggc gcaaatgtca gccactgtca ggcataccag atagtgcaga cgattttccg
     6841 tttgaacttc ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg cggcgttgtg
     6901 ctgtcaccga catcagcaag tgctggcgaa ccagccaggc aaccagctgt gtttcgcgtg
     6961 agttcagccc gtggagttct gcaaaatgca ctacatcctg agcaccgaga atggagtggt
     7021 cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc agctcagttg
     7081 acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt tcttcactgg
     7141 caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg taggcgtgga
     7201 acagatcaaa ctgcatctgc ccgacgatat gcgaccattg cggcatatac gcgccgagca
     7261 cgctatggcg atgcattggc aatagcccgc gccgcaccgc tccggggtga cgcagaatgc
     7321 tcaaaaacag ttttcgtgct tccggaatat tacacagcgg ttgttgcaga tggcgacggg
     7381 catggcgtaa ctggcgcagc gtggtggagt aaatgccggt gatcgcactg ttgcgcacca
     7441 tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt tcatcacgca
     7501 ggtcgattag cgtaccgcgt agctgaaact catcgtcgat tggacgtggt ttttcgtcgg
     7561 cgggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg agttcactga
     7621 cgcggcgtgt aacgcggaag taatccttca tcatccgctc gaccggttcg ttgccttcac
     7681 cactgtaatt cagacgctga gcgacgctaa gttggcgatc gaataacagg cgattatcgt
     7741 aacggctgac gaccagatgc agggcaaagc gaatacgcca cagtatatgc agacattcgt
     7801 ttaattccgc ccgctccgct gaggttaaga agccaaaccc aaccatttca tccagcgatg
     7861 ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg gatatcgcgc aagccgccag
     7921 ggctactttt gatgtctggt tcaagattgt agctggtgcc gtggtaacgc tgatggcgct
     7981 ggttctgttc ttcaactttc gccgcgtaga acttgtcgga aggccagaat ccttcgctaa
     8041 aaatatgttt ttgcagttcg aggaagagcg caacatcgcc aattaataag cgggattcga
     8101 ttaaattggt ggcgacggtt aaatccgata acccttccag catgcactct tcaagcgtgc
     8161 gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc tcgcccactt
     8221 tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg atgtcagaaa
     8281 gtggatgcag ctcgccacga ccgtagccac cgacggcgac caatgccagg tcggcaatct
     8341 ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtcgataaac tcggtgcgcg
     8401 cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc caacgctgga
     8461 aagtatcgat atgggctttt atcccaccga cggttaattc atcacggggc cagacgcatg
     8521 gattttgcgg ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt tctggaaggg
     8581 tattcattgt gcgccaccca taagattaaa ttatcgcatt aaaaaagccg gcattcgccg
     8641 gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt gcgtagcgtc
     8701 agaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc agacaagctg
     8761 cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt tttacccgcg
     8821 ttgaccattg gctcgatggt gaacgtcatc ccaggtttca gtacgacgtt ggtttcacgg
     8881 gagtcatagt gcagcacctg cggttcttca tggaagccgc gaccaatacc gtgtccgcaa
     8941 tattcacgaa cgacggagaa gccttctgct tcgataaact tctgaatcgc cgcaccgatt
     9001 tcgcgcaggt taatgcctgg tttgaccatg cgcagcgcca ggtacaggct ttcttgcgtg
     9061 atgcggcaca gacgttcgcc catgatggtc ggcttaccga cgataaacat tttcgaggta
     9121 tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc gccatctttc
     9181 agcagcttcg catcgtccgg gataccgtgg cacaccactt cattaataga gatgcaaacg
     9241 gatttcggat agccgtgata gccgaggcag gcagaaaccg cgtgttgttc attaacaatg
     9301 taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac atacggttcg
     9361 atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc gatatcttct
     9421 ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggtgata ccgacaatat
     9481 atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt ctgaatttcg
     9541 ccaaacgtgc cactgaacgt tttctataat agaaaattca acgtctgatg ctgtacacag
     9601 cgccaacaat tattggtgtc catgacgtat ttgtggtata aagcgcgccg gacttccgat
     9661 ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa caacacacac
     9721 gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac gtggaggcat
     9781 aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg cgacatgctc
     9841 aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat gaagccgttc
     9901 atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt accgatgttc
     9961 aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat ccttttcgtt
    10021 ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg cgaccagttc
    10081 ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt tcgtcagtcc
    10141 atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga aaagctgacc
    10201 aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag cctgggcggt
    10261 atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga ccacgaacac
    10321 attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt tgataccaac
    10381 tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat ccgtgctgtg
    10441 accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag gccgttctca ggatctggct
    10501 tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcttgataac tcccccaaaa
    10561 tagttcgagt tgcagaaagg cggcaagctc gagaattccc gggagcttac atcagtaagt
    10621 gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg aaatatgaag ggggagagcc
    10681 cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc ctttttcact
    10741 tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt agaatggctg
    10801 aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc atgatggatt
    10861 gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa aacatgcgta
    10921 agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac ggcgtgatca
    10981 aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag actgacttcg
    11041 ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca gctgttgctg
    11101 gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt gttgcgctgg
    11161 tagcgaaaat tggtgagaac atcaacattc gccgcgttgc tgcgctggaa ggcgacgttc
    11221 tgggttctta tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa ggcgctgacg
    11281 aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa ttcatcaaac
    11341 cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg gatatcgcga
    11401 tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc atgaagaaat
    11461 tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc aaaactgttg
    11521 gtcagctgct gaaagagcat aacgctgaag tgactggctt catccgcttc gaagtggggg
    11581 aaggcatcga gaaagttgag actgactttg cagcagaagt tgctgcgatg tccaagcagt
    11641 cttaattatc gaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat cttgtaaatt
    11701 cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag tattaattca
    11761 tttcaatcgt tgacagtctc aggaaagaaa catggctacc aatgcaaaac ccgtctataa
    11821 acgcattctg cttaagttga gtggcgaagc tctgcagggc actgaaggct tcggtattga
    11881 tgcaagcata ctggatcgta tggctcagga aatcaaagaa ctggttgaac tgggtattca
    11941 ggttggtgtg gtgattggtg ggggtaacct gttccgtggc gctggtctgg cgaaagcggg
    12001 tatgaaccgc gttgtgggcg accacatggg gatgctggcg accgtaatga acggcctggc
    12061 aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg ctattccatt
    12121 gaatggcgtg tgcgacagct acagctgggc agaagctatc agcctgttgc gcaacaaccg
    12181 tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt accaccgact cagcagcttg
    12241 cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag ttgacggcgt
    12301 gtttaccgct gatccggcga aagatccaac cgcaaccatg tacgagcaac tgacttacag
    12361 cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg gccttcacgc tggctcgtga
    12421 ccataaatta ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc gccgtgtggt
    12481 aatgggtgaa aaagaaggga ctttaatcac ggaataattc ccgtgatgga taaataaggg
    12541 taagattccg cgtaagtatc gcgggggcgt aagtctggtt ataaggcgtt attgttgcag
    12601 gcagtttggt cacggccagc gcgcagcaac cggagcgtac aaaagtacgt gaggatggcg
    12661 agcactgctc ggggccaaaa tggcaaataa aatagcctaa taatccagac gattacccgt
    12721 aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg tctggtctga
    12781 ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag atgctgaagt
    12841 acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac gcacgggtcg
    12901 tgcttctccc agcctgctgg atggcattgt cgtggaatat tacggcacgc cgacgccgct
    12961 gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca acgtgtttga
    13021 tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg gcctgaaccc
    13081 gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag aacgtcgtaa
    13141 agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag tacgtaacgt
    13201 gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga tcagcgaaga
    13261 cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa tcaagaaaat
    13321 tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc ttgaacgaca
    13381 aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt ttattctgtc
    13441 tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc tcgattggtt
    13501 gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt gcgctggtgg
    13561 caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc cgctatgccg
    13621 taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa cagggtagcc
    13681 gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt gaggatgttg
    13741 atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt gctgcgatcc
    13801 gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc ggacgtctgt
    13861 ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc gaacataacg
    13921 ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct gaccttgagc
    13981 aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt gagacgccat
    14041 tgcgcgattt ggcaacaatg acgcccgatc aagcctgccg tcatccgaac tggtcgatgg
    14101 gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa tacattgaag
    14161 cgcgttggct gtttaacgcc agcgccagcc agatggaagt gctgattcac ccgcagtcag
    14221 tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg ggggaaccgg
    14281 atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac tctggcgtga
    14341 agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat tatgatcgtt
    14401 atccatgcct gaaactggcg atggaggcgt tcgaacaagg tcaggcagcg acgacagcat
    14461 tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc cgctttacgg
    14521 atatcgctgc gttgaattta tctgtactgg aaaaaatgga tatgcgcgaa ccacaatgtg
    14581 tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa gaggtgatgc
    14641 gtctcgcaag ctgaggataa tccggctaca gagagtcgcg ctatttgtta gcgtagggct
    14701 tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg tcaggtatgc
    14761 cgtggtttta cacggctttt ttttgtatag gcttcagtat tcctgagtac cgtaaaccct
    14821 gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa ccacttagcg aaaaattgcc
    14881 agcgcatggc tgccgtcatg ttgcgatcat tatggacggc aatggtcgct gggcaaaaaa
    14941 gcaagggagg attcgtgcct ttgggcataa agccggggca aaatccgtcc gccgggctgt
    15001 ctcttttgcg gccaacaacg gtattgaggc gttaacgctg tatgccttta gtagtgaaaa
    15061 ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa ctgtttgtgt gggcgctcga
    15121 tagcgaagta aaaagtctgc accgacataa cgtgcgtctg cgtattattg gcgataccag
    15181 tcgctttaac tcgcgtttgc aagaacgtat tcgtaaatct gaagcgctaa cagctgggaa
    15241 taccggtctg acgctgaata ttgcggcgaa ctacggtgga cgttgggata tagtccaggg
    15301 agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg caaccagatc agatagatga
    15361 agagatgcta aaccagcatg tctgtatgca tgaactggcc cctgtagatt tagtaattag
    15421 gactgggggg gagcatcgca ttagtaactt tttgctttgg caaattgcct atgccgaact
    15481 ttactttaca gatgttctct ggcctgattt cgatgaacaa gactttgaag gggcgttaaa
    15541 tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag cccggtgatg aaacagcctg
    15601 atggggggcg cttttgctga agtatcgcct gatatctgct tttgtgttaa tacccgtcgt
    15661 catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc atcgtaacgc tggtggtctg
    15721 catgctggca gcgtgggaat ggggacagct tagcggtttt accactcgtt cgcagcgagt
    15781 atggttggcg gtgttatgcg ggttattgtt ggcgctgatg ctttttctgt tgccggaata
    15841 tcaccgaaat attcatcaac cgctggttga aatctcactt tgggcttcgt tgggttggtg
    15901 gattgtcgcg ctattgctgg tgctgtttta cccaggttcc gcagcaatct ggcgtaactc
    15961 taaaacattg cgccttattt ttggcgtgct aaccattgtt cccttcttct ggggcatgct
    16021 ggcgttacgg gcctggcact atgacgagaa tcattacagt ggcgcaatat ggctgctcta
    16081 tgtcatgatc ctggtatggg gcgctgactc cggcgcatat atgtttggca aattgtttgg
    16141 taaacataag ctggcaccga aggtttctcc gggtaaaacc tggcaaggct ttataggtgg
    16201 actagcgacc gcagcggtaa tctcatgggg ttatggcatg tgggcgaatc tcgacgtcgc
    16261 tcccgtcacc ttactcattt gctctattgt cgcagcgtta gcctcagtgc tcggcgatct
    16321 gaccgagagt atgtttaagc gtgaagcagg aattaaggac agcggtcatt taattccagg
    16381 acacggtggt attttagatc gtattgatag cctgacggct gcggtaccgg tctttgcttg
    16441 cttgttgtta ctggtattca ggacgcttta acggaaggta atatgctgag ttttctctgg
    16501 gatttggctt cgttcatcgt tgcactgggt gtacttatca ccgtgcatga atttggtcat
    16561 ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt tctcaatagg gtttggtaag
    16621 gcgctctggc ggcgaactga taagctcggc accgaatatg ttatggccct gattccgctg
    16681 ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg tcgttccgga actccgccac
    16741 catgccttca ataataaatc tgttggccaa cgagcggcga ttattgccgc aggtccggtt
    16801 gcaaacttca tttttgctat ctttgcctac tggctggttt ttattattgg tgtgcctggc
    16861 gtacgtccgg tggttggtga aatagcagcc aattcgatag ctgcggaagc acaaattgca
    16921 ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc ctgattggga tgccgtgcgt
    16981 ttgcagttgg tcgataaaat tggcgatgaa agcaccacca ttacagtagc gccatttggc
    17041 agcgaccaac ggcgggatgt aaagctcgat ttacgtcact gggcgtttga gcctgataaa
    17101 gaagatccgg tatcttcgct ggggattcgt cctcgtgggc cgcaaattga acctgtactg
    17161 gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt tgcaagcagg cgacaggatc
    17221 gttaaagtcg atggtcagcc cttaacgcag tgggtgacct ttgtgatgct tgtccgggat
    17281 aacccgggta aatccttagc gttagaaatc gaaaggcagg ggagtccctt gtctttgaca
    17341 ttaatcccgg agagtaaacc gggtaatggt aaagcgattg gttttgtcgg tattgagccg
    17401 aaagtcattc ctttgccaga tgagtataaa gttgtacgcc agtatgggcc gttcaacgcc
    17461 atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc tgacggtcag tatgctggga
    17521 aaattgatca ccggtgatgt gaaactgaac aacctcagtg ggccgatctc tatcgccaag
    17581 ggggctggga tgacagcgga actcggggta gtttattacc tgccgtttct tgcgcttatt
    17641 agcgtgaact tagggataat taacctgttt ccgttgcccg tacttgacgg ggggcatctg
    17701 ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat ccgagcgggt tcaagacttt
    17761 tgttatcgca ttggctcgat tctgctggtg ctgttaatgg ggcttgcact tttcaatgat
    17821 ttctctcggt tatgagagtt agttaggaag aacgcataat aacgatggcg atgaaaaagt
    17881 tgctcatagc gtcgctgctg tttagcagcg ccaccgtata cggtgctgaa gggttcgtag
    17941 tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt tggtgcggca ctcctcagta
    18001 tgccggtgcg cacaggcgac acggttaatg atgaagatat cagtaatacc attcgcgctc
    18061 tgtttgctac cggcaacttt gaggatgttc gcgtccttcg tgatggtgat acccttctgg
    18121 ttcaggtaaa agaacgtccg accattgcca gcattacttt ctccggtaac aaatcggtga
    18181 aagatgacat gctgaagcaa aacctcgagg cttctggtgt gcgtgtgggc gaatccctcg
    18241 atcgcaccac cattgccgat atcgagaaag gtctggaaga cttctactac agcgtcggta
    18301 aatatagcgc cagcgtaaaa gctgtcgtga ccccgctgcc gcgcaaccgt gttgacctaa
    18361 aactggtgtt ccaggaaggt gtgtcagctg aaatccagca aattaacatt gttggtaacc
    18421 atgctttcac caccgacgaa ctgatctctc atttccaact gcgtgacgaa gtgccgtggt
    18481 ggaacgtggt aggcgatcgt aaataccaga aacagaaact ggcgggcgac cttgaaaccc
    18541 tgcgcagcta ctatctggat cgcggttatg cccgtttcaa catcgactct acccaggtca
    18601 gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa cattaccgaa ggcgatcagt
    18661 acaagctttc tggcgttgaa gtgagcggca accttgccgg gcactccgct gaaattgagc
    18721 agctgactaa gatcgagccg ggcgagctgt ataacggcac caaagtgacc aagatggaag
    18781 atgacatcaa aaagcttctc ggtcgctatg gttatgccta tccgcgcgta cagtcgatgc
    18841 ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt gaacgttgat gcgggtaacc
    18901 gtttctacgt gcgtaagatc cgttttgaag gtaacgatac ctcgaaagat gccgtcctgc
    18961 gtcgcgaaat gcgtcagatg gaaggtgcat ggctgggaag cgatctggtc gatcagggta
    19021 aggagcgtct gaatcgtctg ggcttctttg aaactgtcga taccgatacc caacgtgttc
    19081 cgggtagccc ggaccaggta gatgtagtct acaaggtgaa agaacgtaac accggtagct
    19141 tcaacttcgg gattggttac ggtactgaaa gtggcgtgag cttccaggct ggcgtgcaac
    19201 aggataactg gttaggtaca ggttatgctg ttggtatcaa cgggaccaaa aacgattacc
    19261 agacctatgc tgaattgtcg gtaaccaacc cgtacttcac tgtagatggt gtaagcctcg
    19321 gtggtcgtct cttctataat gacttccagg cagatgacgc cgacctgtcc gactatacca
    19381 acaagagtta tggtacagac gtgacgttgg gcttcccgat caacgaatat aactcgctgc
    19441 gtgcaggtct gggttatgta cataactccc tgtccaacat gcagcctcag gttgcgatgt
    19501 ggcgttatct gtactctatg ggtgaacatc cgagcacctc tgatcaggat aacagcttca
    19561 aaacggacga cttcacgttc aactatggtt ggacctataa caagcttgac cgtggttact
    19621 tcccgacaga tggttcacgt gtcaacctga ccggtaaagt gaccattcct ggatcggata
    19681 acgaatacta caaagtgacg ttagacacgg cgacttatgt gccgatcgat gacgatcaca
    19741 aatgggttgt tctggggcgt acccgctggg gttatggtga tggtttaggc ggcaaagaga
    19801 tgccgttcta cgagaacttc tatgccggtg gttccagcac cgtgcgtggc ttccagtcca
    19861 atacaattgg tccgaaagca gtttacttcc cgcatcaggc cagtaattat gatccggact
    19921 atgattacga atgtgcgact caggacggcg cgaaagacct gtgtaaatcg gatgatgctg
    19981 taggcggtaa cgccatggcg gttgccagcc tcgagttcat caccccgacg ccgtttatta
    20041 gcgataagta tgctaactcg gttcgtactt ccttcttctg ggatatgggt accgtttggg
    20101 atacaaactg ggattccagc caatattctg gatatccgga ctatagtgat ccaagcaata
    20161 tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc attggggccg ttggtgttct
    20221 cctacgccca gccgttcaaa aagtacgatg gagacaaggc agaacagttc cagtttaaca
    20281 tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag tggtagtgta gcgatgactt
    20341 taggcgatca atataagatc gccgggccac gcaaagaact gcaccctccg gtgcaaatgg
    20401 gatggtaagg agtttattgt gaaaaagtgg ttattagcgg caggtctcgg tttagcactg
    20461 gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca atatgggcag cctgttccag
    20521 caggtagcgc agaaaaccgg tgtttctaac acgctggaaa atgagttcaa aggccgtgcc
    20581 agcgaactgc agcgtatgga aaccgatctg caggctaaaa tgaaaaagct gcagtccatg
    20641 aaagcgggca gcgatcgcac taagctggaa aaagacgtga tggctcagcg ccagactttt
    20701 gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc gttccaacga agaacgcggc
    20761 aaactggtta ctcgtatcca gactgctgtg aaatccgttg ccaacagcca ggatatcgat
    20821 ctggttgttg atgcaaacgc cgttgcttac aacagcagcg atgtaaaaga catcactgcc
    20881 gacgtactga aacaggttaa ataagtaatg ccttcaattc gactggctga tttagcgcag
    20941 cagttggatg cagaactaca cggtgatggc gatatcgtca tcaccggcgt tgcgtccatg
    21001 caatctgcac aaacaggtca cattacgttc atggttaacc caaaataccg tgagcattta
    21061 ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg atcttccttt cgcgaaaagt
    21121 gccgcgctgg tagtgaagaa tccctacctg acttacgcgc gcatggcgca aattttagat
    21181 accacgccgc agcccgcgca gaacattgca cccagtgcgg tgatcgacgc gacggcgaag
    21241 ctgggtaaca acgtatcgat tggcgctaac gcggtgattg agtccggcgt tgaactgggc
    21301 gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa acagcaaaat cggtgcaggt
    21361 tcgcgtctct gggcgaacgt aaccatttac catgagatcc agatcggtca gaattgcctg
    21421 atccagtccg gaacagtggt aggcgcagac ggctttggtt atgccaacga tcgtggtaac
    21481 tgggtgaaga tcccacagat tggtcgcgta attattggcg atcgcgtgga gatcggtgcc
    21541 tgcacaacca tcgatcgcgg cgcgctggat gacactgtta ttggcaatgg cgtgatcatt
    21601 gataaccagt gccagattgc acataacgtc gtgattggcg acaatacggc ggttgccggt
    21661 ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact gcatgatcgg cggagccagc
    21721 gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg ttacgggcat gggtatggtg
    21781 atgcgtccca tcactgaacc aggcgtctat tcctcaggca ttccgctgca acccaacaaa
    21841 gtctggcgca aaaccgctgc actggtgatg aacattgatg acatgagcaa gcgtctgaaa
    21901 tcgcttgagc gcaaggttaa tcaacaagac taacgttcca tcttttgttc gccaaacttt
    21961 acggcctgtc tcattcttac gattgcggca ggccgtgtta ttattgtcgt ttcttatatt
    22021 ttgacaggaa gagtatcttg actactaaca ctcatactct gcagattgaa gagattttag
    22081 aacttctgcc gcaccgtttc ccgttcttac tggtggatcg cgtgctggat tttgaagaag
    22141 gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga gccattcttc cagggccatt
    22201 tccctggaaa accgattttc ccgggtgtgc tgattctgga agcaatggca caggcaacag
    22261 gtattctggc gtttaaaagc gtaggaaaac tggaaccggg tgagctgtac tacttcgctg
    22321 gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg cgatcaaatg atcatggaag
    22381 tcactttcga aaaaacgcgc cgcggcctga cccgttttaa aggggttgct ctggtcgatg
    22441 gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag ccgggaggcc tgatacgtga
    22501 ttgataaatc cgcctttgtg catccaaccg ccattgtgga agagggcgcg tcaattggcg
    22561 cgaacgcaca cattggtcct ttttgtatcg ttggacccca tgtcgaaatt ggtgagggta
    22621 ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa aattggccgc gataatgaga
    22681 tttatcagtt cgcctccatc ggcgaagtta accaggatct gaaatatgct ggcgaaccga
    22741 cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag cgtcaccatt catcgtggca
    22801 cagtccaggg cggtggattg acgaaggtgg gcagcgacaa cttactgatg atcaacgcgc
    22861 acattgcgca cgattgtacg gtaggtaacc gctgtattct cgccaacaac gcaacgctgg
    22921 cgggtcacgt atcggttgac gacttcgcga tcatcggcgg catgaccgca gtccatcagt
    22981 tctgcatcat tggtgcgcac gtgatggttg gcggctgctc cggtgtggcg caggacgtcc
    23041 ctccttatgt cattgcgcag ggtaaccacg caacgccgtt cggtgtcaat atcgaagggc
    23101 tgaagcgccg cggattcagc cgtgaggcga ttaccgctat ccgcaatgcg tataagctga
    23161 tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga aattgctgaa ctggcggaaa
    23221 catatccgga agtgaaagcc tttaccgatt tctttgcacg ctcaacgcgc ggtctgattc
    23281 gttaatgact gaacagcgtc cattaacgat tgccctggtc gccggagaaa cctccggcga
    23341 tatcctgggg gccggtttaa tccgcgctct gaaagaacat gtgcccaacg cccgctttgt
    23401 tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa gcttggtacg aaatggaaga
    23461 actggcggta atgggcattg ttgaagtgct cggtcgtctg cgtcgcttac tgcatattcg
    23521 tgccgatctg acaaagcgtt ttggcgaact gaagccagat gtttttgttg gtattgatgc
    23581 gcccgacttc aatattactc ttgaaggtaa cctcaaaaag cagggtatca aaaccattca
    23641 ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc gttttcaaaa taggcagagc
    23701 caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg ttttatgaca aatacaatgt
    23761 accgtgccgc tttatcggtc ataccatggc tgatgccatg ccgttagatc cagataaaaa
    23821 tgccgcccgt gatgtgctgg ggatccctca cgatgcccac tgcctggcgt tgctaccggg
    23881 gagccgtggt gcagaagttg aaatgcttag tgccgatttc ctgaaaacgg cccagctttt
    23941 gcgccagaca tatccggatc tcgaaatcgt ggtgccactg gtgaatgcca aacgccgcga
    24001 gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctt tcagttcatt tgctggatgg
    24061 gatgggccgt gaggcgatgg tcgccagcga tgcggcgcta ctggcgtcgg gtacggcagc
    24121 cctggagtgt atgctggcga aatgcccgat ggtggtggga tatcgcatga agccttttac
    24181 cttctggttg gcgaagcggc tggtgaaaac tgattatgtc tcgctgccaa atctgctggc
    24241 gggcagagag ttagttaaag aattattgca ggaagagtgt gagccgcaaa aactggctgc
    24301 ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac gcgatgcacg ataccttccg
    24361 tgaactgcat cagcagatcc gctgcaatgc cgatgagcag gcggcacaag ccgttctgga
    24421 gttagcacaa tgatcgaatt tgtttatccg cacacgcagc tggttgcggg tgtggatgaa
    24481 gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg cggtgatcct tgacccggcg
    24541 cgcccgattg ccgggctgaa tgattccaaa aagctgagcg aaaaacgccg tctggcgctc
    24601 tatgaagaga tcaaagagaa agcgttgagc tggagtctgg gccgcgcgga accccacgaa
    24661 atcgacgagc tgaacattct tcatgcgacc atgctggcga tgcagcgtgc cgtcgctggg
    24721 ctgcatattg cgccggaata tgtgttgatt gatggtaacc gctgcccgaa attaccgatg
    24781 cctgcgatgg ctgtggtgaa aggcgatagc cgcgtaccgg aaatcagtgc cgcgtctatc
    24841 ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg atattgtttt cccgcaatat
    24901 ggttttgccc aacacaaagg gtacccaacc gcttttcatc tggaaaaact ggctgaacac
    24961 ggcgcgaccg aacaccatcg gcgcagcttt gggcctgtca aacgcgcact gggacttgcg
    25021 tcctgattct tgtgtcgaga ttaagtaaac cggaatctga agatgtctga accacgtttc
    25081 gtacacctgc gggtgcacag cgactactcg atgatcgatg gcctggccaa aaccgcaccg
    25141 ttggtaaaaa aggcggcggc gttgggtatg ccagcactgg cgatcaccga tttcaccaac
    25201 ctttgtggtc tggtgaagtt ctacggagcg ggacatggcg cagggattaa gcctatcgtc
    25261 ggggcagatt ttaacgtcca gtgcgacctg ctgggtgatg agttaaccca cctgacggta
    25321 ctggcggcga acaataccgg ctatcagaat ctgacgttgc tgatctcaaa agcgtatcag
    25381 cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact ggcttatcga attaaacgaa
    25441 gggttgatcc ttctttccgg cggacgcatg ggcgacgtcg gacgcagtct tttgcgtggt
    25501 aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag aacacttccc ggatcgctat
    25561 tttctcgagc tgatccgcac cggcaggccg gacgaagaaa gctatctgca cgcggcggtg
    25621 gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacca acgacgtgcg ctttatcgac
    25681 agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc acgacggctt taccctcgac
    25741 gatcctaaac gcccgcgtaa ctattcgccg cagcaatata tgcgtagcga agaggatatg
    25801 tgcgagctgt ttgccgacat ccccgaagct cttgccaaca ccgttgagat cgccaaacgt
    25861 tgtaacgtaa ccgtgcgtct tggtgaatac ttcctgccgc agttcccgac cggggacatg
    25921 agcaccgaag attatctggt caagcgtgca aaagagggcc tggaagagcg tctggccttt
    25981 ttattccctg acgaggaaga acgtgttaag cgccgcccgg aatatgacga acgtctggag
    26041 actgaacttc aggttatcaa ccagatgggc ttcccgggct acttcctcat cgttatggaa
    26101 tttatccagt ggtcgaaaga taacggcgta ccggtagggc caggccgtgg ctccggtgcg
    26161 ggttcactgg tggcctacgc gctgaaaatc accgacctcg atccgctgga atttgacctg
    26221 ctgttcgaac gtttccttaa cccggaacgt gtctccatgc ctgacttcga cgttgacttc
    26281 tgtatggaga aacgcgatca ggttatcgag cacgtagcgg acatgtacgg tcgtgatgcg
    26341 gtatcgcaga tcatcacctt cggtacaatg gcggcgaaag cggtgatccg cgacgtaggc
    26401 cgcgtgctgg ggcatccgta cggctttgtc gatcgtatct cgaaactgat cccgcccgat
    26461 ccggggatga cgctggcgaa agcgtttgaa gccgagccgc agctgccgga aatctacgaa
    26521 gcggatgaag aagttaaggc gctgatcgac atggcgcgca aactggaagg ggtcacccgt
    26581 aacgccggta agcacgccgg tggggtggtt atcgcgccga ccaaaattac cgattttgcg
    26641 ccgctttact gcgatgaaga gggcaaacat ccggtcaccc agtttgataa aagcgacgtt
    26701 gaatacgccg gactggtgaa gttcgacttc cttggtttgc gtacgctcac catcatcaac
    26761 tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg gcgagccgcc gctggatatc
    26821 gctgcgatcc cgctggatga taagaaaagc ttcgacatgc tgcaacgctc ggaaaccacg
    26881 gcggtattcc agcttgaatc gcgcggcatg aaggacctga tcaagcgtct gcaacctgac
    26941 tgcttcgaag atatgatcgc actggtggca ctgttccgcc ccggcccgtt gcagtcaggg
    27001 atggtggata actttatcga ccgtaaacat ggtcgcgaag agatttccta tccggacgta
    27061 cagtggcagc atgaaagcct gaaaccggta ctggagccaa cctacggcat catcctgtat
    27121 caggaacagg tcatgcagat tgcgcaggtg ctttctggtt ataccctcgg tggcgcggat
    27181 atgctgcgtc gtgcgatggg taagaaaaag ccggaagaga tggctaagca gcgttctgta
    27241 tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac tggcgatgaa aatcttcgac
    27301 ctggtggaga aattcgcggg ttacggattt aacaaatcgc actctgcggc ctatgctttg
    27361 gtgtcatatc aaacgttatg gctgaaagcg cactatccgg cggagtttat ggcggcggta
    27421 atgaccgccg atatggacaa caccgagaag gtggtgggcc tggtggatga gtgctggcgg
    27481 atggggctga aaatcctgcc accagatata aactccggtc tttaccattt ccacgtcaac
    27541 gacgacggcg aaatcgtgta tggtattggc gccatcaaag gggtaggtga aggtccgatt
    27601 gaggccatca tcgaagcccg taataaaggc ggctacttcc gcgaactgtt tgatctctgc
    27661 gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg aaaaactgat catgtccggg
    27721 gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga actcgctggg tgatgcgtta
    27781 aaagcggcag atcaacacgc gaaagcggaa gctatcggtc aggccgatat gttcggcgtg
    27841 ctggccgaag agccggaaca aattgaacaa tcctacgcca gctgccaacc gtggccggag
    27901 caggtggtgt tagatgggga acgtgaaacg ttaggtctgt accttacggg acaccctatc
    27961 aaccagtatt taaaagagat tgagcgttat gtcggaggcg taaggctgaa agacatgcac
    28021 ccgacagaac gtggtaaagt catcacggct gcggggctcg ttgttgccgc gcgggttatg
    28081 gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg atgaccgttc cgggcggctg
    28141 gaagtgatgt tgtttactga cgccctggat aaataccagc aattgctgga aaaagaccgc
    28201 atacttatcg tcagcggaca ggtcagcttt gatgacttca gcggtgggct taaaatgacc
    28261 gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat atgctcgcgg gcttgctatc
    28321 tcgctgacgg acaggcaaat tgatgaccag cttttaaacc gactccgtca gtctctggaa
    28381 gcccaccgct ctgggacaat tccagtacat ctctactatc agagggcgga tgcacgcgcg
    28441 cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg atcgtttatt aaacgatctc
    28501 cgtggcctca ttggttcgga gcaggtggaa ctggagtttg actaatacag gaatactatg
    28561 agtctgaatt tccttgattt tgaacagccg attgcagagc tggaagcgaa aatcgattct
    28621 ctgactgcgg ttagccgtca ggatgagaaa ctggatatta acatcgatga agaagtgcat
    28681 cgtctgcgtg aaaaaagcgt agaactgaca cgtaaaatct tcgccgatct cggtgcatgg
    28741 cagattgcgc aactggcacg ccatccacag cgtccttata ccctggatta cgttcgcctg
    28801 gcatttgatg aatttgacga actggctggc gaccgcgcgt atgcagacga taaagctatc
    28861 gtcggtggta tcgcccgtct tgacggtcgt ccggtgatga tcattggtca tcaaaaaggt
    28921 cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc cagcgccaga aggttaccgc
    28981 aaagcactgc gtctgatgca aatggctgaa cgctttaaga tgcctatcat cacctttatc
    29041 gacaccccgg gggcttatcc tggcgtgggc gcagaagagc gtggtcagtc tgaagccatt
    29101 gcacgcaacc tgcgtgaaat gtctcgcctc ggcgtaccgg tagtttgtac ggttatcggt
    29161 gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg ataaagtgaa tatgctgcaa
    29221 tacagcacct attccgttat ctcgccggaa ggttgtgcgt ccattctgtg gaagagcgcc
    29281 gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg ctccgcgtct gaaagagctg
    29341 aaactgatcg actccatcat cccggaaccg ctgggcggcg ctcaccgtaa cccggaagcg
    29401 atggcggtat cgttgaaagc gcaactgctg gcggatctgg ccgatctcga tgtgttaagc
    29461 actgaagatt taaaaaatcg tcgttatcag cgcctgatga gctacggtta cgcgtaattc
    29521 gcaaaagttc tgaaaaaggg tcacttcgg
//
LOCUS       DAJTYZ010000041        29242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000041 DAJTYZ010000000
VERSION     DAJTYZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.041"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..739)
                     /locus_tag="OO596_001746"
     CDS             complement(<1..739)
                     /locus_tag="OO596_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR6717617.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFR"
     gene            complement(981..1187)
                     /locus_tag="OO596_001747"
     CDS             complement(981..1187)
                     /locus_tag="OO596_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6717618.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            1500..1589
                     /gene="kdpF"
                     /locus_tag="OO596_001748"
     CDS             1500..1589
                     /gene="kdpF"
                     /locus_tag="OO596_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6717619.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1589..3262
                     /gene="kdpA"
                     /locus_tag="OO596_001749"
     CDS             1589..3262
                     /gene="kdpA"
                     /locus_tag="OO596_001749"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6717620.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            3285..5333
                     /gene="kdpB"
                     /locus_tag="OO596_001750"
     CDS             3285..5333
                     /gene="kdpB"
                     /locus_tag="OO596_001750"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR6717621.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            5342..5914
                     /gene="kdpC"
                     /locus_tag="OO596_001751"
     CDS             5342..5914
                     /gene="kdpC"
                     /locus_tag="OO596_001751"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6717622.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5907..8591
                     /gene="kdpD"
                     /locus_tag="OO596_001752"
     CDS             5907..8591
                     /gene="kdpD"
                     /locus_tag="OO596_001752"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR6717623.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            8588..9265
                     /gene="kdpE"
                     /locus_tag="OO596_001753"
     CDS             8588..9265
                     /gene="kdpE"
                     /locus_tag="OO596_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR6717624.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            9540..9644
                     /locus_tag="OO596_001754"
     CDS             9540..9644
                     /locus_tag="OO596_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6717625.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9955..12153
                     /gene="speF"
                     /locus_tag="OO596_001755"
     CDS             9955..12153
                     /gene="speF"
                     /locus_tag="OO596_001755"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR6717626.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            12150..13469
                     /gene="potE"
                     /locus_tag="OO596_001756"
     CDS             12150..13469
                     /gene="potE"
                     /locus_tag="OO596_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6717627.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(13525..15165)
                     /gene="pgm"
                     /locus_tag="OO596_001757"
     CDS             complement(13525..15165)
                     /gene="pgm"
                     /locus_tag="OO596_001757"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6717628.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(15191..15736)
                     /gene="seqA"
                     /locus_tag="OO596_001758"
     CDS             complement(15191..15736)
                     /gene="seqA"
                     /locus_tag="OO596_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6717629.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15921..16685
                     /gene="ybfF"
                     /locus_tag="OO596_001759"
     CDS             15921..16685
                     /gene="ybfF"
                     /locus_tag="OO596_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6717630.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16825..17118
                     /gene="ybfE"
                     /locus_tag="OO596_001760"
     CDS             16825..17118
                     /gene="ybfE"
                     /locus_tag="OO596_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6717631.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            17258..17788
                     /gene="fldA"
                     /locus_tag="OO596_001761"
     CDS             17258..17788
                     /gene="fldA"
                     /locus_tag="OO596_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6717632.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17998..18084
                     /gene="uof"
                     /locus_tag="OO596_001762"
     CDS             17998..18084
                     /gene="uof"
                     /locus_tag="OO596_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6717633.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            18077..18523
                     /gene="fur"
                     /locus_tag="OO596_001763"
     CDS             18077..18523
                     /gene="fur"
                     /locus_tag="OO596_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6717634.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18608..18934)
                     /locus_tag="OO596_001764"
     CDS             complement(18608..18934)
                     /locus_tag="OO596_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717635.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18984..20390)
                     /gene="chiP"
                     /locus_tag="OO596_001765"
                     /pseudo
     CDS             complement(18984..20390)
                     /gene="chiP"
                     /locus_tag="OO596_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20968..22632)
                     /gene="glnS"
                     /locus_tag="OO596_001766"
     CDS             complement(20968..22632)
                     /gene="glnS"
                     /locus_tag="OO596_001766"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6717636.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22891..24837)
                     /gene="nagE"
                     /locus_tag="OO596_001767"
     CDS             complement(22891..24837)
                     /gene="nagE"
                     /locus_tag="OO596_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6717637.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            25170..25970
                     /gene="nagB"
                     /locus_tag="OO596_001768"
     CDS             25170..25970
                     /gene="nagB"
                     /locus_tag="OO596_001768"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6717638.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            26030..27178
                     /gene="nagA"
                     /locus_tag="OO596_001769"
     CDS             26030..27178
                     /gene="nagA"
                     /locus_tag="OO596_001769"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6717639.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            27187..28407
                     /gene="nagC"
                     /locus_tag="OO596_001770"
                     /gene_synonym="nagR"
     CDS             27187..28407
                     /gene="nagC"
                     /locus_tag="OO596_001770"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6717640.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            28455..29207
                     /gene="nagD"
                     /locus_tag="OO596_001771"
     CDS             28455..29207
                     /gene="nagD"
                     /locus_tag="OO596_001771"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR6717641.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         6813 a         7501 c         7713 g         7215 t
ORIGIN      
        1 gccggaagtg acgcccggca ccatccgtca cgccggtgat ttcgccgctg aattcaccgg
       61 cggcttcgcg gtggaacgtc tgtgtgcgcc cgaagcggtc caccagcccg gtcagtaccc
      121 ggtacggcgg cagcggcgca ggcagcacct catccgcttc cggcacccgc tcacaccagc
      181 cgagcagcca ccacggcccc tgcggactgt ttgtcgccag ataacgatgc ggacttaagc
      241 ggagttcttc cggcagcgcc tgccagagtg cggccagccg gtgaccttca tccagtttcg
      301 ccacgccgcc gcgcaccagc cagagtgatt cgctgcggct gtaaccgtcc tcaccgggaa
      361 acaggtgctc aaaatacagg cttctgccgc cgttatcact gagtatcagt gtgttatcgc
      421 gcagctgtaa gcggatatcc gcaggcattt tccagccggg gccgaggctc cccaccggcg
      481 cgggcgtttt tgtccggtaa ctgctgtagg tgcgggagag gatgaacggc agcggggcgg
      541 gcagggcgat gtcggtttca ccgggaagga cctttgcacc gagcagggga ttgaccggat
      601 ggccggacgt cactccgccg gggcacaccg aacaggccac accggtgggg gcaccgatgc
      661 gcaccccggc tgaaccctgg acaatgctac cgccatactg cgtcatgtcg ccctgacgcg
      721 ccgccggttt tccgctcatt tctcatcctt gttttatcgg gttatttgtc cgctgacagg
      781 caagacccta cagatttata agaaagcctt aagtgagata tccgtcagat agaaacaatt
      841 aggaaaatat ttatatatat ttcatgaata tatgtttcga ttggtataac ggtgtctaaa
      901 tattcctctg aacatcttcc tgatacaaaa aaccgccagc gtttccgctg gcggttttca
      961 atgtattcag aactcagcaa tcagtaaaaa tcaccggttg ccttttcggc ctgatccatc
     1021 cacaccggtt tatcgctggt tttcgaccag acgcggtgca ggtagctgta aaagcgggcg
     1081 cggtctttcc agaaaagcat gaaaggcagc gccagaacgc ccgctgcaac cgcatatgta
     1141 cggcgtaaaa agataagcca tgcaggatat tctctgtaga gttccatact tttctccccc
     1201 ttttgtatct acccggtgaa tagcaccgga aaaatgaatt tgtttatctg atgaaaatag
     1261 taccgccttt tgtgtaattt tactactcat ccgaccactt atttttgctt attgatggtt
     1321 tatttacatt cactctgtaa ttaagttaca caaaagttaa attaatacta aacattagtt
     1381 aaatcatggc ttttgccatt tttatacttt ttttacaccc cgcccgcaga tttttgcgaa
     1441 atctttgcag ccagaattct actcttccgg tatcactttt aggccactgg aggtgcactg
     1501 tgagtgcagg cgtgatagcc ggcgtgttgc tggtgttttt attactgggt tatttggttt
     1561 atgccctgat caatgcggag gcgttctgat ggctgcgcaa gggttcttac tgattgccac
     1621 gtttttactg gtattaatgg tgctggcgcg tcctttaggc agcgggctgg cgcggctgat
     1681 taatgacatt cctcttccag gtacagcggg cgttgaacgt atactctttc gtttacctgg
     1741 cgtctctgac catgagatga actggaagca atatctttgt gccattctcg gcctgaacat
     1801 gctggggctg gcggtgctgt tttttatgtt gctcggtcag cactatctgc cgcttaatcc
     1861 acagcagttg ccggggctgt cgtgggatct ggcgctgaat accgccgtca gctttgtcac
     1921 gaataccaac tggcaatctt atagcggtga aaccacgttg agctatttca gccagatggc
     1981 aggcttaacg gtacaaaact ttctttctgc cgccagcggg attgcggtga tttttgcctt
     2041 catccgtgcg tttactcgcc agagcatgag cacactcggg aatgcctggg tcgatctgct
     2101 acgcatcacg ttatgggtgc tagtccctgt ggcgttgttg atcgcactgt tttttattca
     2161 acaaggtgcg ctgcaaaact ttctgcccta tcaggcagtg aataccgttg aaggagcgca
     2221 acagctgtta cccatgggac cagtagcttc tcaggaagcg atcaagatgc tcggtactaa
     2281 cggcggtggc ttctttaatg ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac
     2341 caacttcgtg cagatgctgg cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg
     2401 tgaagtgacg ggcgatcgcc gccaggggcg catgttgctg tgggcgatgt cagtgatttt
     2461 tgtcatctgc gtaggcgtgg ttatgtgggc agaagttcag ggtaatcctc atctgctggc
     2521 gctcggcgca gacagcagca tcaatatgga aggtaaagag agccgtttcg gcgtgctggt
     2581 cagtagcctg tttgcggtcg tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca
     2641 tgattcgttt accgctctcg gcggcatggt gccgatgtgg ctaatgcaaa ttggtgaagt
     2701 ggtgttcggc ggtgtcggtt ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt
     2761 gtttattgcc gggctgatga ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt
     2821 acgcgagatg aaactgactg cactggcgat tctggttact ccaacactgg tactgatggg
     2881 cgcggcgttg gcgatgatga ccgacgccgg acgtagcgcc atgcttaacc ctggcccgca
     2941 tggttttagc gaagtgctgt acgccgtgtc gtccgccgct aacaacaacg gcagcgcctt
     3001 tgccggatta agcgccaact ctccgttctg gaactgttta ctggcgttct gcatgtttgt
     3061 cggtcgcttc ggggtgatta tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa
     3121 gagccaaccc gccagctccg gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt
     3181 aatcggcacc gtgttgctgg ttggcgcact gacctttatc cctgccctgg cgcttggtcc
     3241 ggtggcggaa tatctctcct gatgatattg agcgagcact gaatatgagt cgtaaacaac
     3301 tggcgctatt cgaaccaaca cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa
     3361 acccgcaggc gcaatggcgc aatccggtga tgtttatcgt ctggatcggc agtctgctga
     3421 ccacctgtat tagcatcgcg atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg
     3481 cggcaattag cggctggctg tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg
     3541 cagaaggccg cagtaaagcg caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg
     3601 cccgcaagct gcgtgagccg aaatatggcg ctgcggcgga caaagttcct gccgatcaac
     3661 tgcgtaaagg tgacatcgtg ctggtagaag ctggcgatat tatcccctgc gatggtgaag
     3721 tcattgaagg cggcgcatcg gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga
     3781 tccgtgaatc cggcggcgat tttgcctccg tcaccggcgg cacgcgtatt ctttctgact
     3841 ggctggtgat tgagtgtagc gttaaccccg gtgagacatt tctggatcgg atgatcgcga
     3901 tggtggaagg cgcacagcga cgcaaaacac cgaacgagat tgccctgact atcctgctga
     3961 ttgctctgac catcgtcttt ttactggcaa ccgccacgct gtggccgttt tccgcgtggg
     4021 gcggtaatgc cgtgagtgta acggtactgg tggcgctgct ggtctgtctg atcccaacca
     4081 ctattggcgg cctgttgtca gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga
     4141 atgtgattgc caccagcgga cgtgcagttg aagcggcagg tgacgttgac gttctgctac
     4201 tggataaaac cggcaccatc acactcggta accgtcaggc gtcggagttt atccccgcgc
     4261 agggcgtgga tgaaaaaacg ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa
     4321 cgccggaagg tcgcagtatt gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg
     4381 atgtgcagtc gctccatgcc acctttgtac cgtttactgc gcaaagccgg atgagcggga
     4441 tcaacatcga caaccgcatg atccgtaaag gttctgtcga tgccattcgc cgccatattg
     4501 aggctaacgg cggtcacttc cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc
     4561 agggagccac gccgctggtg gtggtggaag gttctcgtgt gctgggcgtt attgcgctga
     4621 aagatatcgt caaaggcggt attaaagagc gcttcgccca gctgcgcaaa atgggcatta
     4681 aaacggtgat gattaccggc gataaccgtc tgactgccgc cgcgatagct gcggaagcgg
     4741 gtgtcgatga ttttctcgcc gaagcaacac cggaggccaa gctggcattg atccgtcagt
     4801 atcaggcgga aggtcgtttg gtagcgatga ccggcgacgg caccaacgat gctccggcgc
     4861 tggcgcaggc agatgtcgcg gtggcgatga actccggcac ccaggcggcg aaagaggcgg
     4921 gcaatatggt ggatctcgac tctaacccga ccaagttgat cgaggtggtg cacattggca
     4981 aacagatgct gatgacccgt ggctcgctga ccaccttcag cattgccaac gatgtggcga
     5041 aatacttcgc cattattccg gcggcgttcg cggcaacata tccgcagtta aatgcgctga
     5101 acatcatgcg cctgcattcg cccgactccg ccattctcag tgcggtcatt ttcaacgcct
     5161 tgattatcgt ctttttgatt cctctggcgt taaaaggcgt gagttataaa ccgcttaccg
     5221 cttctgccat gttgcgccgt aacttatgga tttacggtct gggtgggctg ctggtgccgt
     5281 ttattggtat caaagtcatt gatttactgc tgaccgtttg cggtctggtg tgaggtttac
     5341 catgagtgga ttacgtccgg cattatcgac atttctcttt ctgttattga ttactggcgg
     5401 cgtttacccg ctgctgacca ccgcactggg gcaatggtgg tttccctggc aggccaacgg
     5461 ttcgttgatc cgtgaaggtg atacggtgcg tggctcggca ttaatcgggc agaattttac
     5521 cggcaacggc tattttcatg gtcgcccgtc agcaacggct gaaatgccct ataatccaca
     5581 ggcttctggc gggagcaatc tggcggtcag taaccctgag ctggataaac taatagccgc
     5641 acgcgttgct gcattacggg ccgctaaccc ggatgccagc gcgagcattc cggttgaact
     5701 ggtgacggca tcggcaagcg ggctggacaa taatatcacc ccgcaagcgg cggcctggca
     5761 gatcccacgc atcgcgaaag cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc
     5821 aaaatacagc caacaaccgc tggtgaaata tatcggccag ccggtcgtca acattgtcga
     5881 actcaatctg gcgctggata agcttgatga ataacgaacc cttacgtccc gaccccgatc
     5941 gtctgctgga acaaactgcc gcgccgcatc gggggaagct gaaagttttc ttcggtgcct
     6001 gtgcaggcgt cgggaagacc tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag
     6061 ggctggatat tgtggttggc gtggtagaaa cccacgggcg aaaagatacc gccgcaatgc
     6121 tggaagggct ggctgttctg ccgccaaaac gcctggcgta ccgtgggcgg cacatctgcg
     6181 agtttgacct cgatgccgcc ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg
     6241 cgcacagtaa tgcgccaggt tctcgtcacc ctaaacgctg gcaggacatc gaagagctgc
     6301 tggaagcagg cattgatgtt ttcactaccg tcaacgttca gcatctggaa agtctgaatg
     6361 atgtggtcag cggcgtcacc ggaattcagg tacgggaaac cgtgcccgat ccttttttcg
     6421 atgccgccga cgacgtggtt ctggtggact tgcccccgga cgatctgcgc cagcggctga
     6481 aagaaggcaa agtctatatt gccgggcagg cggagcgcgc cattgaacat tttttccgca
     6541 aaggcaatct gatcgccctg cgcgaactgg cactgcgccg tactgccgat cgcgttgatg
     6601 agcaaatgcg cgcctggcgg gggcatccta gcgaagagaa agtctggcac acgcgcgacg
     6661 cgatcctttt atgcatcggc cataacaccg gtagtgagaa gctggtccgc gcggcggcgc
     6721 ggctggcgtc acggctgggt agcgtctggc acgcggtgta tgttgaaacc cctgccctgc
     6781 atcgcttacc ggagaaaaaa cgtcgtgcaa ttctcagcgc cttacgtctg gcgcaggaac
     6841 tgggcgcgga gacggcaaca ctttctgatc cagcggaaga gaaagcggta gtgcgttatg
     6901 cccgtgaaca taatctcggc aagattattc tcggtcgccc ggcctcgcgc cgctggtggc
     6961 gtcgggaaac gtttgctgac cgactggcgc gcatcgcccc cgatctcgat caggtgctgg
     7021 tcgcgcttga tgaaccaccc gcccgcacga ttaacaacgc gccggatagc cgctctttta
     7081 aagacaagtg gcgggtacag attcagggat gcgtggttgc cgccgcgtta tgcgccgtta
     7141 tcaccttaat tgccatgcag tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt
     7201 atctgcttgg cgtggtggtg gtggcgctat tttatggacg ctggccttcg gtggttgcca
     7261 ccgtcattaa tgtggtgagt ttcgatctct tttttatcgc cccacgcggc acgctcgccg
     7321 tctctgatgt gcaatatctg ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg
     7381 ggaaccttac tgctggcgtg cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca
     7441 cacggcactt atatgaaatg tcgaaagctc tggcggtggg ccgcagtccg caggacatcg
     7501 ccgccaccag cgaacaattt attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc
     7561 ccgatgacaa cggtaaattg cagccgttaa cacatccgca aggaatgacg ccatgggacg
     7621 atgccatcgc gcagtggagt tttgataaag gcctgcctgc gggcgcgggc accgacacgt
     7681 tacccggtgt accgtaccag attttgccgc taaaaagcgg cgagaaaacc tacgggctgg
     7741 tggtggtgga gccggggaat ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg
     7801 agacgtttac gctgttagtc gccaatgccc ttgagcggct gacgctaacc gccagcgaag
     7861 aacaggcgcg gatggcaagc gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt
     7921 cgcatgattt acgcacgccg cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg
     7981 atctggcaag cgaaggatca ccccacgccc gccaggccag cgagattcgt cagcatgtgc
     8041 tgaacactac ccgactggtg aataatctac tggatatggc gcgaattcag tccggcggct
     8101 ttaatttgaa gaaagagtgg ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc
     8161 tggaaccggg tttatcgtcg cccatcaatc tttctctgcc agaaccgctg accttaatcc
     8221 acgttgacgg gccactcttt gaacgggtgc tgattaatct gctggagaac gcggtgaaat
     8281 atgcgggtgc gcaggccgaa attggtatcg atgcccactt tgagggcgaa aatctacaac
     8341 tggatgtctg ggataacggc cccggtcttc cgccaggcca ggagcagacg atatttgata
     8401 agtttgctcg cgggaataaa gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt
     8461 gtcgggcgat tgtggatgta cacggcggca ccattaccgc gttcaaccga ccggaaggtg
     8521 gtgcctgttt tcgtgttaca cttccccagc aaaccgctcc tgaacttgaa gactttcatg
     8581 aggatatgtg acaaacgttc tgattgttga agatgaacag gctattcgtc gctttctgcg
     8641 cacggcgctg gagggcgacg ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt
     8701 gctggaagcg gcaacccgta agccagattt gattattctc gatctcggcc tgcccgatgg
     8761 tgatgggatt gagtttatcc gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct
     8821 ttccgcacgc agcgaagaga gcgacaagat cgccgcgctg gatgccggag cggatgatta
     8881 tctgagtaag ccgtttggca ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg
     8941 ccactccgcc accgccgcgc ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt
     9001 agccgcccgc gtgattcacc ggggtgagga agaggtgcat ctcacaccaa ttgagttccg
     9061 cctgctggcg gtgctgctca acaatgccgg aaaagtactc acccagcgcc agctccttaa
     9121 ccaggtgtgg gggccaaacg cggtcgaaca cagtcactat ttgcgtattt atatgggaca
     9181 tctgcgacaa aaactggaac aggatccggc ccggcctcgc cacttcatta ctgaaaccgg
     9241 tattggatat cggtttatgc tttgaattgt tattcaataa attcgcttaa atagtgatta
     9301 atttattatt aaacctgcgc cgaatattat tatttggcag gtttaatttc ttaacgcaaa
     9361 tataaaacac gaaattacaa catttaagta acaaaacagc atctaatatg gcgatattca
     9421 taattaatga tttattatct ggggtatgat cgttttttgt tgatcttctt cacagattat
     9481 agccatttca tggatagaat aactctacct tcaactgaca cagcaagagg taaaggtaaa
     9541 tggaaaataa taaccgctta atgcctcata taaggcggac aacccatatc atgaagtttg
     9601 cccatcgtaa tagttttgac tttcatttct ttaatgcccg ctagtcttct gactaaaggg
     9661 caccccaacg tacaggtctc cctgacttta agcattacag gttaatacct gtattcctcg
     9721 gtgctcatat actgctaacc cttttaaact ctaaataatt cgagtcgcag cacttgcaac
     9781 ttgaggtatg acgagtatag ccagttaccg ggctggtctg ggttattgca tctgcaaaaa
     9841 gcaaactact gatttattta tcagcggtgg agctttgctt tttttccggc gtgatcgatt
     9901 tctcctttga gaaattgagg acctgctatt acctgaaata aagagatgaa taaaatgtca
     9961 gaattaaaaa ttgccgttag ccgttcttgc ccggattgtt tttccactca tcgtgcatgc
    10021 gtgaatattg acgaaagtaa ttatattgac gttgccgcca ttattttatc agtcaatgat
    10081 gttgaacgtg gaaaactcga tgaaatagac gctactggct atggcattcc tgtttttatt
    10141 gcaacggaaa atgaagaacg tgtaccagca gaatatctgc cacgtatttc tggtgtcttc
    10201 gagcattgcg aatcacggaa agagttttat ggacgtcagt tagaaaccgc tgctagccat
    10261 tatgaaacgc aactgcgccc gcccttcttc cgcgcactgg tcgattatgt caatcagggc
    10321 aatagcgctt ttgactgccc gggtcatcag ggaggtgagt ttttccgtcg tcatccggca
    10381 gggaatcagt tcgtggagta cttcggagaa acgctgttcc gttccgactt atgcaacgca
    10441 gacgtagcaa tgggcgatct gctgattcac gaaggcgcgc catgcattgc acagcaacat
    10501 gcggcaaaag tgtttaatgc cgataaaaca tacttcgttc taaatggcac ttcatcttct
    10561 aacaaagtgg ttttaaacgc cctgctaaca ccgggtgatc tggtgctgtt tgatcgcaat
    10621 aaccacaaat ctaaccacca cggagcgttg ctacaggctg gtgcaacacc ggtttatctg
    10681 gaaacggcac gtaacccgta tggctttatc ggtggcattg atgcgcactg ttttgaagaa
    10741 agttacctgc gtgagctgat cgcggaagtc gcaccgcagc gggcaaaaga ggctcgtcct
    10801 ttccgcctcg ctgtgattca gttaggcacc tacgacggta cgatttataa cgcccgccag
    10861 gtggtggata aaattggtca tctgtgtgac tacattctgt ttgactccgc atgggttggc
    10921 tatgagcaat ttatcccgat gatggctgac tgttcgccgc tgttgctgga tcttaatgag
    10981 aacgatccgg gtattctggt tacgcaatcc gtacataaac agcaggctgg tttttctcaa
    11041 acttcacaaa tccataaaaa agacagccac atcaaagggc aacagcgtta tgtaccgcac
    11101 aaacgcatga acaacgcctt tatgatgcac gcctccacca gcccgttcta tccgctgttt
    11161 gccgcactgg atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa tatgtggatg
    11221 gattgtgtgg taaatggcat taatgcccgc aaactgatcc tcgataactg tcagcatatt
    11281 cgtccgttcg tacctgaact ggtggatggt aaaccctggc agtcgtatga aacagcgcaa
    11341 attgcggttg atctgcgctt cttccagttt gtaccagggg aacactggca ttcttttgaa
    11401 ggctatgcag agaatcaata ctttgtcgat ccttgcaaac tgttgctgac aaccccaggt
    11461 attgatgcac gtaacggcga atatgaagcg ttcggtgtac ccgcgacgat tcttgctaac
    11521 ttcctgcgcg aaaatggcgt agtgccggaa aaatgcgatc ttaactccat cctcttcctg
    11581 ctgactccgg cagaagatat ggccaaactt cagcaacttg ttgccctgct ggtacgcttc
    11641 gaaaaactgc ttgagtccga cgcgccatta gcagaagtgc tgccttccat ctacaaacag
    11701 catgaagagc gctacgccgg ttataccctg cgtcagttgt gccaggaaat gcatgatttg
    11761 tatgcccgcc acaacgtgaa acaactgcaa aaagagatgt tccgcaaagc gcacttccca
    11821 cacgtcagca tgaatccgca agaagccaac tacgcctatt tacgcggtga agtggagctg
    11881 gttcgtctgc cggatgccga aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg
    11941 ggtgtgctgt gtgttgttcc gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc
    12001 gctctggaag aagggatcaa cctgctgcca ggttttgcac cggagctgca gggtgtctat
    12061 atcgaagaac atgatggtcg taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg
    12121 caaagcaccc tgttgaaagg ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg
    12181 tcgttcagtt aaccatactg acgatggtca acatgatggg ctccggtatc atcatgctgc
    12241 cgacaaagct tgccgaagtt gggacaattt caattatctc ctggctggtg acagccgtag
    12301 gctcaatggc actggcatgg gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg
    12361 gtatgggcgg ttatgccgag tatgctttcg gtaaatccgg taactttatg gcgaactata
    12421 cctacggcgt ctcactgctg attgctaacg tcgcgattgc tatttcggcg gttggttacg
    12481 gcaccgaact gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg accatcgggg
    12541 tgctgtggat ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg caaatcagta
    12601 gcattaccgt gtggggggtc attattccgg tggtaggcct gtgcattatc ggctggttct
    12661 ggtttagccc gacgctttac gttgattcct ggaatccgca tcatgcaccg ttcttcagtg
    12721 cagtaggttc ttccatcgct atgacgctgt gggcttttct cggtctggag tctgcgtgtg
    12781 cgaatactga tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta ctcggcggta
    12841 cgttaggtgc ggcggtgatt tatatcgtct ccaccaacgt gattgcaggg attgtgccta
    12901 atatggagct ggcaaattca acggcaccat ttggcctggc cttcgcacag atgttcacac
    12961 cagaagtggg taaagtcatt atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg
    13021 gctggcagtt caccattgcc caggtgttta agtcttcatc tgatgaaggc tacttcccta
    13081 aaattttctc ccgtgtaacc aaagtggatg caccggtgca gggaatgttg accattgtga
    13141 ttatccagag cgggttggcg ctgatgacga ttagcccgtc gctaaacagt cagttcaacg
    13201 tgctggttaa cctggccgtg gtgaccaata tcattccgta tattctgtca atggcggcat
    13261 tagtcattat tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg
    13321 ttgcttttgt tggcgcgatg tatagctttt atgcgctgta ctcatccggg gaagaagcca
    13381 tgctgtacgg ttccatcgtg accttccttg gttggacact gtatggtctg gtatcaccac
    13441 gctttgaact gaaaaataaa cacggttaaa aaagcaaagt aaaaaagggc gatcttgcga
    13501 ccgccctttt tttattaaat gtgtttacgc gtttttcaga acttcgctaa caatctcaac
    13561 cgcttctttc tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc agtagatctt
    13621 atatgcgtct tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt
    13681 cagaccgccg atagaagcac cattgcccgg tgccgccgtc aggcgcgcgg tgatcgggtc
    13741 gcctgccagg gtgctggcgc tcaccatttc cggagacagc ttagacagcg ccgctttttg
    13801 tgcggaagtc gcagctgcct gcaaacggtt gtagctcggc gcaccaaagc gttttgccag
    13861 ttcgttgtag tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg ccgccagcag
    13921 acacatgatg atgccgtctt tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga
    13981 agcccctgcg ctctcttcgc cgccaaagcc gaagctgccg tcgaacagac catcaacaaa
    14041 ccatttgaaa cctaccggga cttctaccag cttgcggccc aagtcattga ccacacggtc
    14101 gatcatcgca gaagaaacca gcgttttacc gacagcaaca tctttgcccc actgcggacg
    14161 atgctggaac aggtagttga tcgccaccgc caggtagtgg ttcggattca tcaaacctgc
    14221 cggagtgacg ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt
    14281 atcacgcaat gccagcaggc ccgccatcgc acactcggag gagcagtcca tacggatcgc
    14341 gccgtcttta tcgaggtgca taaagcggaa ggtttgatcg acctgatcgt taacgatggt
    14401 caggttgagg ttgtaatact cgccaattcg cttccagtat tcgataccgg aaccacccag
    14461 cggatcaacg cccagcgtca ggcccgtttt ctgaatcgcc gccatatcaa cgatatcggc
    14521 cagaccttcc acaaactgct gcaccagatc ctgctctttc acatgaccgg atgccatcgc
    14581 ttcgtcgagg gagatacgct tcacgccttt caggccatcg gccatcagtg cgttggcacg
    14641 atcttccacc actttagtga cgttggtatc agccgggcca ccatttggcg gattgtactt
    14701 gataccacca tcttccggcg ggttatggga gggtgtaatc acgataccgt ctgccagcgg
    14761 gccacctttt ttattatgaa ccaggatggc attggaaacg gcaggcgtcg gagtgaagcc
    14821 attgttttcc tgcacaatga catcaacgcc gttcgctgcc agcacttcca gtacggaaat
    14881 gaacgcaggc tcggacaggg cgtgagtatc tttacccaca tagcaagggc cagtgatgcc
    14941 gtttttcgca cgttcttcag caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa
    15001 gctgtggcgc gctgcactgc cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc
    15061 attccctgct tctggtttca gtacataata ttgcgccgtc agttgggcga cgttaatcaa
    15121 atcactctgt tgtgcaggtt ggcctgcacg attgtggatt gccattgctt tgtcctttgt
    15181 ctgcaacgtt ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg
    15241 actgcatgat gtgttcgatc atgctgcatt tacggccggt gttggtgttg gtgatcaccc
    15301 aatacggcgt gcctggcaca tgtttcggct tggtctgatt accatttttc agcagcgttt
    15361 gttcatctgc cgcaaagtaa acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa
    15421 acgcctgggc gtcaagagaa tatagtgtag acaacagcag cataaagcga ttgaccgctc
    15481 gcttttgctc tgcgtattcg tcagaaagca gaagttcacg cattgcgcga accttgtctt
    15541 taatcgtttt gaccggcttc gcttcgacga tagcaggtga cgcaacgcga acctctttcg
    15601 tcaccggagc agcaggctgt gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg
    15661 atgcgctctc gccgatatgc ttagtgtggc tggcaatata gctgtagagt tcatcatcaa
    15721 cttcaatcgt tttcatctta atccagtaca gtgtcttagc tgaatacaag tcgccaagag
    15781 tatatgaata aatcccggca gcggatagcg tcaagcctgg cttgcagaaa aactcggaat
    15841 aaaccttatt tgttgcagcc ctgtggcaca atggtcaaca taatacccta acccgacaac
    15901 gcgaaaaaaa gaactttgcc atgaaattga atatccgcgc gcaaactgca caaaaccagc
    15961 acaataattc tcccatcgtt cttgtccatg gtctgtttgg tagcctcgac aaccttggcg
    16021 tactggctcg cgatctggta aacgatcaca atatcatcca ggttgatatg cgtaaccacg
    16081 gtctttcacc gagagatccg gtaatgaatt acccggcgat ggcgcaggat cttgttgata
    16141 ctctggatgc acagcagatc gacaaagcaa catttatcgg tcactccatg ggcggtaaag
    16201 cggtaatggc acttacggca ctggcccgcg atcgcatcga taaactggtg gcgatcgata
    16261 tcgcaccggt cgactatcac gtacgccgtc atgatgagat tttcgcggct atcaacgcgg
    16321 tcagtgaatc ggacgcacaa actcgccagc aagcagcagc aataatgcac cagcatctta
    16381 atgaagaagg ggtgattcag tttctgttga aatcttttgt tgacggggag tggcgcttta
    16441 acgtgccggt attgtgggat cagtatccgc atattgtggg ttgggagaaa atcccggcat
    16501 gggatcaccc tgccctgttt atccctggcg gcaattctcc gtatgttagc gagcagtacc
    16561 gtgatgattt actggcacaa tttccacagg cacgggcgca tgtgattgca ggcgcgggtc
    16621 actgggtcca tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg
    16681 attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg
    16741 tgactcatcg cgctgatgta ttatggcgcg ctatccatcc gggcagaggc ctggctgatt
    16801 gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac gacattagat
    16861 ctgttcgcgc acgagcgtcg accgggacga ccgaaaacta atccgctttc gcgcgatgaa
    16921 cagctgcgta ttaataaacg caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt
    16981 gtcgaactga agctgaacgc ggaagctgtc gaggcgctga acgagctggc ggagtcgcgc
    17041 aatatgagcc gtagcgaact gatcgaagag atgttaatgc agcaactggc ggcgctgcgt
    17101 agtcagggca tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt
    17161 gctgattccg caagactgcc tgttctgcta tgattgcctt tatccgtggg caattttcca
    17221 cccccatttc aataagtttc aagaggttat ttcactcatg gctatcactg gcatcttttt
    17281 cggcagcgac accggtaata ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa
    17341 agacgttgcc gatgtccatg acattgcaaa aagcagcaaa gaagatctgg aagcttatga
    17401 cattctgctg ctgggcatcc caacctggta ttatggcgaa gcgcagtgtg actgggatga
    17461 cttcttcccg actctcgaag agattgattt caacggcaaa ctggttgcgc tgtttggttg
    17521 tggtgaccag gaagattacg ccgaatattt ctgcgacgca ttgggcacca tccgcgacat
    17581 cattgaaccg cgcggtgcaa cgatcgttgg tcactggcca actgcgggct atcatttcga
    17641 agcatcaaaa ggtctggctg atgacgacca ctttgtcggt ctggctatcg acgaagaccg
    17701 tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt
    17761 gcatctcgac gaaattctca atgcctgatg tgatgcggcg tagactcatg tctacgccgt
    17821 attaatagat aatgccaatc aaaataattg ctacaaattt gtaacttttg ctgttgtacc
    17881 tgtacaatgt cccggtgttc aagtggcctt gccgttgtaa atgtaagctg tgccacgttt
    17941 ttattaacaa tatttgccag ggacttgtgg ttttcattta ggcgtggcaa ttctataatg
    18001 atacgcatta tctcaagagc aaattctgtc acttcttcta atgaagtgaa ccgcttagta
    18061 acaggacaga ttccgcatga ctgataacaa taccgcccta aagaaagctg gcctgaaagt
    18121 aacgcttcct cgtttaaaaa tcctagaagt tcttcaggaa ccggacaacc atcacgtcag
    18181 tgcggaagat ttatacaaac gtctgatcga tatgggtgaa gaaattggtc tggctacggt
    18241 atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca attttgaagg
    18301 cggtaaatcc gtatttgaac tgacacagca acatcaccac gatcacctga tctgcctcga
    18361 ctgcggcaag gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc
    18421 cgcaaaacat ggcattcgcc tgactaacca cagtctctat ctttacggtc actgtgccga
    18481 aggcgattgc cgcgaagatg aacatgcgca cgaaggcaaa taagccagcc tgaatgagaa
    18541 aaagccaacc tgcgggttgg cttttttatg caagggaaat aagaaaagcg agagttacag
    18601 ctctcactta tttgttatta ctgcgaattt cctgccaaac tttatcacaa tcggctttca
    18661 ccgcctgatc atttccggtt tgcgttgcac gcagatactg ctgataatcc agtacgcgga
    18721 cactttcctg gtcggcaaaa tgctgatgtt gtttctcttt cttcagcacg tttaacacgc
    18781 tctggcaggc ttcaattttt tctggcgaac cttgtgcggt gttgatacag gcgctgtatg
    18841 cctctttcag acgactgtct tcctgtggcg cggttgattg cgcacaagct accagccccg
    18901 atgccattat ggcgatgaga atcagttttt tcatagcaaa ttcctcaaac gtgcggcagg
    18961 cacaaaacct gccgcgtcgg gcatcagaag atggtgaatg gtgcgattac cataaatttc
    19021 acgtcacgct catcctggaa gatgttgccg taaccaccgc cccagcttgg gatgtcggag
    19081 tggttgtcgt attcggtgaa gtgcagtttg aacatcgtgc ctttggcgcg accgtcctga
    19141 atggtgtaga ccgcatccag gctgtatgca gactcttcaa tagtccggtt tttgtcgtag
    19201 tacgcatccg gattgctctg ctaggtcgca ggtttagcat cccatgcgta aacgtaggaa
    19261 gcgccgatgg cgaagcctgg aagattccag tttttcaggt catacatcgc accgaagaag
    19321 accgcttttt cgccgttagc gttgaagtcg gaacggttat cccaccagat atccaggcgg
    19381 ccgttggagg aagcgtaggt tggagtcata cgttgcagga agtatccctg ctgaccgtca
    19441 gccttaaccc aggtgccttc gaggcgcaaa tccactacgt cagccgcccg gtaaccaaag
    19501 gtcaacgctt gcagccaggc ggtgccgtca taaaggtcgt tgacgctgcg atcgtcaact
    19561 ttatcgcgcg taccatagaa ctggtagctg gtggttaacg ggctaccggc gatatcaaat
    19621 ttgtagctgg ctttggcaaa atattgatcg atataccctt ccgcctgacc aaatgccgct
    19681 tccagtacga agttattttt gaagtcgtat ttcgccccaa gggagtgcag ataatcaact
    19741 ttggtggttt tatcgttctg ataaaactca tccatttcca gatgccacgg cgctttgtat
    19801 tcgttggtcc acatgtagga gaaactcaac gcaccagcat cgccgtaatc aaaattcgcc
    19861 ccggcttccg ccccctgata agtacctggc ataaagctcc agtgcggcgc taacagcgtt
    19921 tgaccagttg gctgaatgta acctgccctc gcccaaaccg gaccgtattt aaatttggcc
    19981 gcagctttat acaggcttat accgcttttg tcgccggacc agtcttcgtc ataggcttta
    20041 ttactttttg aaaacgcgat ttcgttcggg tgggagctgt cgccgttttc cgccatttca
    20101 atcgccgtaa acgcagcaat atcaaggccg aacatatcag cagcataacc ggactgaaaa
    20161 tcgaggttgg cattccaggt ggagtgagaa aggttggttt tgtatttgtc gccgtcggta
    20221 acatctttac ggtcgcgttc acgctgccag taatatatac cgccggttaa ggttgaatcg
    20281 tcgatgaatc cttcggcgcg agcctccggc attgccataa agcccgacat tgctgtaaca
    20341 ccggcgatag ccagcgccag cgtactacgt ttgccactaa acgtacgcat gggttaatcc
    20401 tctttgacgt ataaattgct gcaccaaagg tgaacagcga aaaaataaaa attaaaaaag
    20461 tagcgttgag aaaagtctcg ctgactacag gaacagacta tttttcgcga cgcgaattat
    20521 caatctcttt ctgtaaccag aatgtaagaa catgagcaac cgcacatatt ttgatgattt
    20581 ttgttacaag gcttaagttt gcggttgaaa ggcttgcaga agaataaaag cgtgaaattc
    20641 cagcaagatg aaagcggtta cattttattt aattatgttt tgttatttta aatttatttt
    20701 atggataaat tgcgaagcgg aattaattcg cgaagaaaag cagaaaaaac gccgctgaac
    20761 gcggcgtttt ggattgtttg catatttctt gccggatgcg gcgtgaacgc cttaaccggc
    20821 ctacaaaagc acgcaaactc aatatattgc agagatcatg taggcctgat aagcgtagtg
    20881 catcaggcaa tttagcgttt gaaactgttt gtctgttcct tgccggatgc gacgtaaacg
    20941 ccccatccgg catagcgaaa cttaaaatta ctcgcctact ttcgcccagg tatcacgcag
    21001 cccaacggtg cggttaaata ccggtttttc cgccgtagaa tggcggctat cgaggcagaa
    21061 gtaaccttca cgctcaaact ggaatgcttt acccgcaacc gcatctttca gcgacggttc
    21121 agcaaagccc tgtttgatca ccagcgattc cgggttaatc accgacagga aatcatccgc
    21181 agcaccaggg ttcggcacgc tgaacagacg gtcgtacaga cggatttcaa ccggcagcgc
    21241 atgtgccgcg ctcacccagt gaataacacc tttgacttta cgaccatctg ccggatcttt
    21301 gcttaaggtg tcggcgtcat aagtacagaa gatggtggtg atattgcctt cggcatcttt
    21361 ctccacgcgt tctgccttaa tgacgtaagc attacgcaga cgcacttctt tacccagcac
    21421 cagacgtttg tactgcttgt tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc
    21481 accgctaaac ggcacctggc ggctacccat ttccggtttg ttcggatggt tcggcatggt
    21541 aaccatttcg ccttcgccct gatagttttc gataaccagt ttcaccggat cgataaccgc
    21601 cattgcgcgc ggggcgtttt cgttgagatc ttcacggatg caggattcca gcgacgccat
    21661 ctcaatggtg ttgtcctgct tggtcacgcc gatgcgtttg cagaactcac gaatagaagc
    21721 agcagtgtaa ccacgacgac gcagaccgga aatggtcggc atacgcgggt catcccagcc
    21781 ttcaacgtgc ttgtcggtca ccagcaggtt cagcttacgc ttggacatca cagtgtattc
    21841 cagattcagg cgcgagaatt cgtactggcg cgggtgaaca ggaatggtga tgttgtccag
    21901 cacccagtcg tacaggcgac ggttgtcctg gaactcaagc gtacacagag agtgcgtaat
    21961 accttccagg gcatcgctga tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt
    22021 gttgccagtc tggtggtgtt cagcaaattt aatgcggtac agcaccggat cgcgcatcac
    22081 gataaacggt gaagccatgt cgattttcgc acgcaggcag gctttacctt cttcaaaacc
    22141 accggcacgc attttttcga acagcgccag gttctcttca acgctgcggt cgcggtacgg
    22201 gctgttttta cccggttgcg tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag
    22261 ttcatcaacg tacgccaggc ctttattgat cagttcgatc gcataggcgt ggagctgatc
    22321 aaaataatcg gaggagtaac ggacgttacc agaccagtga aaacctaacc actctacgtc
    22381 gtttttgatc gactcaacat actcgatatc ttcttttacc gggttagtgt cgtcgaaacg
    22441 caggttgcac tggcctttat agtcctgggc gatcccgaag ttcaggcaga tagatttcgc
    22501 atggccaata tgcagatagc cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg
    22561 cttaccactg gccagatctt catcgatgat ctgacggata aagttagtcg ggcgggcttc
    22621 tgcctcactc atcgtggatt cctcaaagcg taaacaacgt ataacggcgt atgatcttat
    22681 aagcgggaca ggctgacaac tgttagttag cgaaaatcga aaatctcact gagattatgg
    22741 ggatgattga tgaaaaaaaa agcggcagag aagatctcca ccgcttttgc cagtcccgcg
    22801 tcattgttgg atgcgcgtct tatccaacca ggactgagct tttgtaggcc tgataagacg
    22861 cgtcaagcgt cgcatcaggc ataaagcaga ttactttttg atttcataca gcggtgtttg
    22921 acccgccaca acattgccct gagctttaat gatcaagcca ctgaaatcgt cgatattgct
    22981 gcaaaccacc gggctaatca tcgaacgggc gttagcgttc aggtaatcca gatccatttc
    23041 cagaatcggt tgccctgcgc tcacctgcgc gccctcttcc accagacgtt taaagccttt
    23101 accttccagc gctacggtgt cgatacccat gtggacgaca atctccgcgc ctttttcggt
    23161 ttctaggcag aacgcgtggt tggtgttgaa gattttcacg attgtccctg cggctggtga
    23221 tacgacgatt ttatctgtcg gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa
    23281 tgcttcgtca gaaacctgat ccagtgccac gacatcaccg gtaatcggcg ataccagctc
    23341 cgcgatagat accgcgtttg gtacagcctg cggttttgct acaggagcgg cagttgccgg
    23401 agttgcttca gctgacgcag ctgctaccgg gccacgggca acgactttct tcatcgcatc
    23461 gccaatggat tctgctttcg cgccaacaat cacctgaatg gtctgtttgt tcagttttac
    23521 tacaccagaa gcacccagac gtttacacat cgcatcgttg acgcgagctg agtcagccac
    23581 ggtaaggcgc agacgggtaa tacaggcgtc aatcgctttc aggttgtcag taccgccaac
    23641 cgcagcaata tagttggttg ccagttgagt cagaccttct tcagtattgc tgttagcctc
    23701 ttcagttacg atctcgtctt ctttatcttc acgacccggc gttttcaggt tgaacatgcg
    23761 gataaccaaa ctgaacacca cgaagtagat agcgaagaag acaacaccca tcaccagcag
    23821 catccagacg ttctggctgg cggccggcag gttatacatc aacgcgtagt cgatagcccc
    23881 cgcagagaaa gagaagccag catggatacc cagcagcgtt gccacaaaca ggctgatacc
    23941 ggtcagcagt gcgtgcagga ggtacagcag cggagcaagg aacatgaaca ggaattccag
    24001 cggctcagtc acaccggtca ggaacgcagt aacagcaaca gaaagcagca tcccgccaac
    24061 catcggacga cgctctttcg gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc
    24121 gaacatcatg atcgggaaga agccggacat gaacatcccc gcggtgccgt caccagcgta
    24181 gaaacggttg atgtcgccgt ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg
    24241 gaaccaggcg atggtgttca gcacctgatg cagaccggtt gggatcagca gacggttgat
    24301 gaaaccaaag ataccggaac ccagcgcgcc tgcagaaacg atccactcgc cgcctgcatg
    24361 gatagcgtgc tgtactggcg gccagacgta accaaaaatg gccgccagca ccaggcagaa
    24421 aaagccggtg gcaatcggca caaagcgttt gccgccgaag aagctcagga agtccggcag
    24481 tttaatatcg gaccaacggt tataggctgc gccaccaacc agaccggtaa tgatacccgc
    24541 cagtacaccc atgttaattt ctgggttgat ggtcaccatc gctttggtta acacaaagta
    24601 acctaccgca cccgccagcg ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac
    24661 accgatggcg aagattaatg cgaggttatc aaaaatcgca ccgcccgcct gggcaataaa
    24721 cgcaacgtta agtaaatctg gctgaccgaa tcgcagcaac agtgccgcca ccggcagcac
    24781 cgcgataggg agctgtaacg ccctaccgag tcgctggaaa aaacctaaaa tattcatctt
    24841 attcccccta cgagaaccct atttggctcg tttaaagccg tatttttatt ttgctgcaaa
    24901 ttgtactgcc gatgttctgt aatcagattg ttagatcatc tgctacagag tgtgtgaaaa
    24961 tttaattcgt atcgcaaatt aaacgcgtgc cttttgtgag ttttgtcacc aaatatcgtt
    25021 atcatcactc ccttttactg gctaaagcag aaaacttatt ttatcattca aaaaatcagg
    25081 tcggattgac gcctgtctgc gcaaatccag gttacgctta aagatgccta atccgccaac
    25141 ggcttacatt ttacttattg aggtgaataa tgagactgat ccccctaact accgctgaac
    25201 aggtcggcaa atgggctgct cgccatatcg tcaatcgtat caatgcgttc aaaccgactg
    25261 ccgatcgtcc gtttgttctg ggcctgccga ctggcggcac gccgatgacc acctataaag
    25321 cgttagtcga aatgcataaa gcaggccagg tcagctttaa gcacgttgtc accttcaaca
    25381 tggacgaata tgtcggtctg ccgaaagagc atccggaaag ctactacagc tttatgcacc
    25441 gtaatttctt cgatcacgtt gatattccag cagaaaacat caaccttctc aacggcaacg
    25501 ccccggatat cgacgccgaa tgccgccagt atgaagaaaa aatccgttct tacggaaaaa
    25561 ttcatctgtt tatgggcggt gtaggtaacg acggtcatat tgcatttaac gaaccggcgt
    25621 cttctctggc ttctcgtact cgtatcaaaa ccctgactca tgacactcgc gtcgcgaact
    25681 ctcgtttctt tgataacgat gtcaatcagg tgccaaaata tgccctgact gtcggtgttg
    25741 gtacattgct ggatgccgaa gaagtgatga ttctggtgct gggtagccag aaagcactgg
    25801 cactgcaggc tgcagttgaa ggttgcgtga accacatgtg gaccatcagc tgtctgcaac
    25861 tgcatccaaa agcgatcatg gtgtgcgatg aaccttccac catggagctg aaagttaaga
    25921 ctttaagata tttcaatgaa ttagaagcag aaaatatcaa aggtctgtaa ttgttatccc
    25981 tgccctggct ccttgctcag ggcaatattt tttaaaatcg gggggcagaa tgtatgcatt
    26041 aacccagggc cggatcttta ccggccacga atttcttgat gaccacgcgg ttgttatcgc
    26101 tgatggcctg attaaaagcg tctgtccggt agcggaactg ccgccagaga tcgaacaacg
    26161 ttcactgaac ggggccattc tctcccccgg ttttatcgat gtgcagttaa acggctgcgg
    26221 cggcgtacag tttaacgaca ccgctgaagc ggtcagcgtg gaaacgctgg aaatcatgca
    26281 gaaagccaat gagaaatcag gctgtactaa ctatctgccg acgcttatca ccaccagcga
    26341 tgagctgatg aaacagggcg tgcgcgttat gcgcgagtac ctggcaagac atccgaatca
    26401 ggcgttaggt ctgcatctgg aaggtccgtg gctgaatctg gtaaaaaaag gcacccataa
    26461 tccgaatttt gtgcgtaagc ctgatgccgc gctggtcgat ttcctgtgtg aaaacgccga
    26521 cgtcattacc aaagtgaccc tggcaccgga aatggttcct gcggaagtca tcagcaaact
    26581 ggcaaatgcc gggattgtgg tttctgccgg tcactccaac gcgacgttga aagaagcgaa
    26641 agccggtttc cgcgcgggga ttacctttgc cacccatctg tacaacgcga tgccgtatat
    26701 taccggtcgt gaaccgggcc tggcgggcgc gatcctcgac gaagctgaca tttattgcgg
    26761 tattatcgct gatggcctgc atgttgatta cgccaacatt cgtaacgcta aacgccttaa
    26821 aggcgacaaa ctgtgtctgg ttaccgacgc caccgcgcca gcaggtgcca acattgaaca
    26881 gttcattttt gcgggtaaaa caatatacta ccgtaacgga ctttgtgtgg atgagaacgg
    26941 tacgttaagc ggttcatcct taaccatgat tgaaggcgtg cgtaatctgg tcgaacattg
    27001 cggtatcgca ctggatgaag tgctgcgtat ggcgacgctc tatccggcgc gtgcgattgg
    27061 cgttgagaaa cgtctcggca cactcgccgc aggtaaagta gccaacctga cctcattcac
    27121 acctgatttt aaaatcacca agaccatcgt taacggtaac gaggtcgtaa ctcaataaga
    27181 gaaagtatga caccaggcgg acaagctcag ataggtaatg ttgatctcgt aaaacagctt
    27241 aacagcgcgg cggtttatcg cctgattgac cagtacgggc caatctcgcg gattcagatt
    27301 gccgagcaaa gccagcttgc ccccgccagc gtaaccaaaa ttacgcgtca gcttatcgaa
    27361 cgcgggctga tcaaagaagt tgatcagcag gcctccaccg ggggccgccg cgctatctcc
    27421 atcgtcaccg aaacccgcaa tttccacgca atcggcgtac ggcttggtcg tcacgacgcc
    27481 accatcaccc tgtttgatct cagcagcaaa gtgctggcag aagaacatta cccgctgccg
    27541 gaacgtaccc agcaaacgct ggaacatgcc ctgttgaatg ccattgctca gtttattgat
    27601 agctaccagc gcaaactgcg cgagctgatc gcgatttcgg tgatcctgcc agggcttgtt
    27661 gacccggaca gcggcaaaat tcattacatg ccgcatattc aggtagaaaa ctgggggctg
    27721 gtagaagctc tggaagaacg ttttaaagtg acctgtttcg ttggtcacga tatccgtagt
    27781 ctggcgctgg cggagcacta cttcggtgca agtcaggatt gcgaagactc cattctggtg
    27841 cgtgtccatc gcggaaccgg ggccgggatt atctctaacg ggcgcatttt tattggccgc
    27901 aacggtaacg tcggtgaaat tggccatatt caggtcgaac cgctgggtga acgctgccac
    27961 tgcggcaact ttggctgcct ggaaactatc gccgccaacg ctgccattga acaacgggtg
    28021 ttgaatctgt taaagcaggg ctaccagagc cgcgtgccgc tggacgactg caccatcaaa
    28081 actatctgca aagccgcgaa caaaggcgat agtctggcgt cggaagtaat tgagtatgtc
    28141 ggtcgtcatc tgggtaaaac catcgccatt gctatcaact tatttaatcc gcaaaaaatt
    28201 gttattgccg gtgaaatcac cgaagccgat aaagtgctgc tccctgctat tgaaagctgc
    28261 attaataccc aggcgctgaa ggcgtttcgt actaatctgc cggtggtacg ttctgagctg
    28321 gatcaccgct cggcaatcgg cgcttttgcg ctggtaaaac gcgccatgct caacggtatt
    28381 ttgctccagc atttgctgga aaattaatgt gcttttatag tggcgcttat tgttgtcaat
    28441 attctgggta gtccatgacc attaaaaatg taatttgcga tatcgacggc gtgctgatgc
    28501 acgataacgt cgccgtaccg ggtgcagcgg aatttttgca cgggattatg gataaaggcc
    28561 tgccgctggt gttgctgacc aactatcctt cgcagaccgg gcaagatctg gcgaaccgct
    28621 ttgccactgc cggtgtcgat gtgccggata gcgtgtttta tacctctgcg atggcgaccg
    28681 ccgatttcct gcgtcgccag gaaggcaaga aagcatatgt ggtgggcgaa ggcgcactga
    28741 ttcatgaact gtacaaagcc ggtttcacta ttaccgatgt gaaccctgat ttcgtgattg
    28801 ttggcgaaac gcgttcctac aactgggaca tgatgcataa agcggcctat ttcgtcgcta
    28861 acggtgcacg ctttatcgcc accaacccgg acacccacgg gcgcggtttt tatcccgctt
    28921 gtggcgcgtt gtgtgcaggg attgagaaaa tctccgggcg caaaccgttc tatgttggta
    28981 agcccagccc gtggatcatc cgcgcagcat taaacaaaat gcaggcgcat tcggaagaaa
    29041 cggtgattgt cggcgataac ttgcgcaccg acattctggc gggcttccag gcaggtctgg
    29101 aaacgattct ggtgctttct ggtgtttcat cgctcgacga tatcgacagt atgccgttcc
    29161 gcccaagctg gatttacccg tcagtcgctg aaatcgacgt tatctgaata ccacccacgt
    29221 ctgcggacgt ggttggctct ga
//
LOCUS       DAJTYZ010000042        28793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000042 DAJTYZ010000000
VERSION     DAJTYZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28793
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.042"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1160)
                     /locus_tag="OO596_001772"
     CDS             complement(<1..1160)
                     /locus_tag="OO596_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6717642.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELR"
     gene            1651..2208
                     /gene="pinE"
                     /locus_tag="OO596_001773"
                     /pseudo
     CDS             1651..2208
                     /gene="pinE"
                     /locus_tag="OO596_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2547..3395)
                     /locus_tag="OO596_001774"
     CDS             complement(2547..3395)
                     /locus_tag="OO596_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6717643.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3604..4239
                     /gene="yfhb"
                     /locus_tag="OO596_001775"
     CDS             3604..4239
                     /gene="yfhb"
                     /locus_tag="OO596_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6717644.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4264..4800
                     /gene="tadA"
                     /locus_tag="OO596_001776"
     CDS             4264..4800
                     /gene="tadA"
                     /locus_tag="OO596_001776"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6717645.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4797..6353)
                     /gene="mltF"
                     /locus_tag="OO596_001777"
     CDS             complement(4797..6353)
                     /gene="mltF"
                     /locus_tag="OO596_001777"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6717646.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQCLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6611..10498
                     /gene="purL"
                     /locus_tag="OO596_001778"
                     /gene_synonym="purI"
     CDS             6611..10498
                     /gene="purL"
                     /locus_tag="OO596_001778"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6717647.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRSGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            11056..12483
                     /gene="qseE"
                     /locus_tag="OO596_001779"
     CDS             11056..12483
                     /gene="qseE"
                     /locus_tag="OO596_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6717648.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12648..13361
                     /gene="qseG"
                     /locus_tag="OO596_001780"
     CDS             12648..13361
                     /gene="qseG"
                     /locus_tag="OO596_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6717649.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13351..14685
                     /gene="glrR"
                     /locus_tag="OO596_001781"
     CDS             13351..14685
                     /gene="glrR"
                     /locus_tag="OO596_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6717650.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14746..15084
                     /gene="glnB"
                     /locus_tag="OO596_001782"
     CDS             14746..15084
                     /gene="glnB"
                     /locus_tag="OO596_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6717651.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15129..16319)
                     /gene="hmpA"
                     /locus_tag="OO596_001783"
     CDS             complement(15129..16319)
                     /gene="hmpA"
                     /locus_tag="OO596_001783"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6717652.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16647..17900
                     /gene="glyA"
                     /locus_tag="OO596_001784"
     CDS             16647..17900
                     /gene="glyA"
                     /locus_tag="OO596_001784"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6717653.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(18098..19291)
                     /locus_tag="OO596_001785"
                     /pseudo
     CDS             complement(18098..19291)
                     /locus_tag="OO596_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19409..22686
                     /locus_tag="OO596_001786"
                     /pseudo
     CDS             19409..22686
                     /locus_tag="OO596_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22783..23766
                     /locus_tag="OO596_001787"
     CDS             22783..23766
                     /locus_tag="OO596_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6717654.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYISAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23789..25300
                     /locus_tag="OO596_001788"
     CDS             23789..25300
                     /locus_tag="OO596_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6717655.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25325..26323
                     /locus_tag="OO596_001789"
     CDS             25325..26323
                     /locus_tag="OO596_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6717656.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26356..27450
                     /locus_tag="OO596_001790"
     CDS             26356..27450
                     /locus_tag="OO596_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6717657.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27462..28334
                     /locus_tag="OO596_001791"
     CDS             27462..28334
                     /locus_tag="OO596_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6717658.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28382..>28793)
                     /locus_tag="OO596_001792"
     CDS             complement(28382..>28793)
                     /locus_tag="OO596_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6717659.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6846 a         7118 c         7827 g         7002 t
ORIGIN      
        1 tgtcgaagct ccgcagaggc actgagtttt tccagccatg cagcgacctg ttcacggaat
       61 ccggaggtat tgcgtgcaga gacggtatcg gaaaggcggt caaggaacgc ggagaatgcc
      121 cgggaattct cctcggtgga aaaagaagtc cattgtgtag aaacctcccg acgatctgct
      181 tcaggaaacc aggctgccac agtttcggga agagggggga tctgaaagtt ctgcccccct
      241 tcagtgatga ctctgatatg gcgcgaatgt aaattttgcg gcaacatctg gcgtaaaaac
      301 agtctgtttt ggtctgacag ggaaatacga aactgaggcc cgttaaaatt tggaaccgaa
      361 gtttgctgta ttaagaatag cagagtttga tctgataagg ggttatttgc aatctcaata
      421 gttatatgag gatctaaacg aaaaagattt gtcgggattg tagttatttg gttgttttca
      481 aaataaaaaa atcttctcat gataatattt tcttggatgt ttggtaagtt gcttaatcta
      541 ttgctatatg caaaaagaag ttgcagtctc ctaggtaaag ggggcagttc agtaagatgg
      601 ttgtgcgcaa ccttaagtat cttaagatta gaaggcagag atggaagagt ttgcagttcg
      661 ttgttcctaa cctcaagttt ttttaaattc aagggtaatg aaggcagtgt ggataaccta
      721 ttatactctg cgcttagtga tttgatggtg tctggtaata acggcagatt ctctaattga
      781 ttttcggaca catccaaagt tattagataa tgtggtaatg cagatatatg tgttaagaaa
      841 ttatctcttg caataagttc tttaatattt tcaggtaaat cagtaaggat ggttaattta
      901 ttacagctca catcaagctt tttaatgcaa gcagggagta caggtaacga ggaaagcgcc
      961 aaggctcgca ggtccagttt atgcccctta gaggtaagac atgcacgaag tcttcttacc
     1021 gcctcagtac gctgttcttc cggtaacccc tgtttctccc aatcatccca ttcggacaaa
     1081 taatggttgg gggatgtaat tgtgctgata tgtggaaata aacaggatga aatatttctg
     1141 agcatattaa tttctcgcat atggatatgg aattagattg tgaataaatt gtatttaatg
     1201 tatctctcct aatgaggagg gtgattcaga atattcgcca taagggaatg agtgttatat
     1261 catgctttct accctggtaa gtatatttgc ttttttcgta tatttggttc atcttttttt
     1321 gatgttttac atattcacaa tgcagaggat ttataccaga agacattagt cttgagtaag
     1381 atactacaac atctccaact attttagcca gcctgactac ttgacttggt aatatttgtt
     1441 gctaataatc atgtataaaa taagcttata tacatagtaa cttcagcaaa ggatttagta
     1501 atgatccaaa tcatatctca atgtactgtt tttttcttta aaaaaacagt acattctact
     1561 ttataatgac tgtgtattcc catggaagga gatcacccaa ttctcccccc cgacttttga
     1621 cgcagtgtct tgttaacagg gggatttacc atgcttattg gctatgtacg cgtatcaaca
     1681 aatgaccaga ataccgatct acaacgtaat gcgttgaact gtgcaggatg cgagctgatt
     1741 tttgaagaca agataagtgg cacaaagtcc gaaaggccgg ggctgaaaaa actgctcagg
     1801 acattatcgg caggcgacac actggttgtc tggaagctgg atcggctggg gcgcagtatg
     1861 cggcatcttg tcgtgctggt ggaggagttg cgcgaacgag gcatcaactt tcgtagtctg
     1921 actgattcaa ttgataccag cacaccaatg ggacgctttt tcttccatgt gatgggtgcc
     1981 ctggctgaaa tggaacgtga actgattgtt gaacgaacaa aagcaggact ggaagctgct
     2041 cgcgcatagg gacgaattgg cggtcgccgt cccaaacttt caccagaata atgggcgcag
     2101 gccgggcgac taattgcatc aggagtttct cgccagaagg tggcgatcat ctatgatgtt
     2161 ggcgaatcga cttttttgta taagaggttt ccagccaggg ttatataaaa ccgcagacac
     2221 ggcgtataca agaacgtgct acggttgact gaaaagttaa tgtggtatgt cacaggcctt
     2281 ttgcgccgta aagccccgtc ctttagggcg cggaggacgt taaagaaagt ggtgttcttt
     2341 gtgttgctga acttatgcaa aatttttttg caccataaat ccaacaacac attcaataac
     2401 acattgctgt atcactatga aaataaatgt gtttttattt gatttcacac atatcacaat
     2461 tgatgcattt tttagtgatt aacaacgcca taaggaaact ctcaaaacaa cacaaaccgg
     2521 gcgggcattg tacgcgcatt ctttactcag accagttttt tcaccagcgc ttcactatga
     2581 cgaatacgtt ctggtgccag ttccagatcc tgctgaatca gcgcaatgaa cagcaaatcc
     2641 gttaacattc cctgagcgtg acaagcagag attgaagcac tgtttgtcgc ttgttcttcg
     2701 gcaatggtat acaggcaatg agaagcacgc tgttgcaggg cattcggagt aaagccggta
     2761 atcgccagca cttttccgcc cactcgcagc atctcatctg ccgccaggtt taactcgcgt
     2821 cgtacaccgg tgtaggaaat ggctaataac agatcgtcag gggacgacgc ctgtactgtt
     2881 gcgagtagcg catgcatatc gcgcactgcg gcagcattga agccaatctt catcagcttc
     2941 caggcaaagt tttgcgccac cagacccgaa gcgccaatac cggtcagaat tatccgccgc
     3001 gcagagcgca acattgttac gcattcatgc agtttctctt cactattaac gtttagcgtt
     3061 gcgtacatag cggcagtatt ttctttaatc agtttttcgc cgaccagccg taacggatca
     3121 tcaccgcgga tttggttatg aatgggcacg gagggcgatt ccggctggct tgccagcgct
     3181 tcactcaacg ccaacttaag cgccggaaaa cctttatagc cgagtttttg cgcgaacttc
     3241 acgacgctgg actgactgac tccggcttcg ttggccagtt gctgagagct taaatggcgc
     3301 gccgtatcag gttgtagcag cagataatcc gccagttttt tatcactctg ggcaagcccc
     3361 tggtaacgct gacggatacg aagtaagccg ttcatatctt tcctcgcgat gaaatagtgg
     3421 cgacaacagg caatatgaga cacacgcgag tgaattttat attccattgc cgctaattgc
     3481 catgaattaa aaattccagt tagccatgat gcggtcgatt gttgccggaa aagtgatgat
     3541 ctatctgtta ggctatgtag tagcgaaaat taatcaggat atttcagtcc agaggagtat
     3601 ggtttggcaa ctcacgagcg tcgtgtggtg ttttttgact tagatggaac attgcatcag
     3661 caggatatgt tcggcagttt tctgcgctat ttactgcgtc gccaaccgct gaatgcgtta
     3721 cttgtcctgc cgttgttacc gattatagcc attgcgttat tgataaaagg tcgtgcggca
     3781 cgctggccga tgagtctgct tctgtggggg tgcacttttg gtcacagcga agcacgttta
     3841 cagacgttgc aggccgattt cgtgcgctgg tttcgcgaca atgttaccgc ctttccgctg
     3901 gttcaggagc gattaaccac ctacctgtta agttccgatg ctgatatctg gttgattacc
     3961 ggctctccgc agccgctggt tgaagcggtt tatttcgata cgccctggct gccgcgggtt
     4021 aatcttatcg ccagccaaat tcagcgtggc tatggtggtt gggtattgac gatgcgttgt
     4081 ctgggacatg aaaaggtcgc acaactggag cgcaaaatcg gcactccgct gcggctgtac
     4141 agtggctata gcgacagtaa tcaggacaat ccgctgcttt atttctgtca gcatcgttgg
     4201 cgagtaaccc cgcgcggtga actccagcaa ctggaataga gtaaagcata gcgtccgtgt
     4261 ataatgcgcc gcgcttttat aaccggagtt ttctttttgt ctgaagtcga atttagccac
     4321 gaatactgga tgcgtcacgc gctgacgctg gcgaaacgtg cctgggatga gcgggaagtg
     4381 ccggtcggcg cggtattagt gcataacaat cgggtaatcg gcgaaggctg gaaccgcccg
     4441 attggtcgcc atgatcccac cgcacatgca gaaatcatgg ccctgcggca gggtggtctg
     4501 gtgatgcaaa attatcgtct gatcgacgcc acgttgtatg tcacgcttga accatgtgta
     4561 atgtgtgccg gagcgatgat ccacagtcgc attggtcgcg tggtctttgg tgcgcgtgac
     4621 gcgaaaactg gcgctgcggg atctttaatg gatgtgctgc atcatccggg tatgaatcac
     4681 cgagtggaaa ttacggaagg aatactggcg gatgagtgcg cggcgttgct cagtgacttc
     4741 tttcgcatgc gccgccagga aattaaagcg cagaaaaaag cgcaaccctc gacggattaa
     4801 ttttgtttct cttcactccc tttcctggaa aacagcagag agggagaatg ggtcaaatag
     4861 tttgatgacg actgggaaag aaacgagaga aaaggaaatt tctctttgcc caactccgta
     4921 ggcgataccg ccggataatc ctgcgccagt tgcatcgccg cttctgtagc ctgcttctct
     4981 ttctcttgca gataacccac caggctaatc tgatacttac gaatattttc gacataagcg
     5041 taggcttcat gtccacgagc gtagccgtaa gtcagcttgc tgtaataggg tttctggcta
     5101 agtaaaggca gacactgttt tacgtcagcc caactgtcag gattcccttt ggtttttgcc
     5161 gtcagggcgc gggcatccag catatgcgca tagcccatat tgtacgcagc gagggcaaac
     5221 cagatccgct cgttctccgg cacactttcc ggcactttac tcatcatatc ctgcaaataa
     5281 cgcacgccac cgctgatgct ctgttcggca tcggtacgat ccgtaatgcc gaggctttgc
     5341 gcggtatttt tggttaacat catcatgccg cgcacacccg tcggtgaagt ggcctgtgca
     5401 tcccagtgcg attcctgata agcaatagcg gccagcaaac gccagtcaat ttcttcggcg
     5461 tatttctcaa acaggggctt taactgcggc agtaccgcat cgacggcgcg taaaaatgtg
     5521 cgcgtatcga cgtaatcaaa atcatcgcca tgccccaggt atttctcttc aatgcgtgcc
     5581 agcgtaccgt cttcattcat ttcgttgaag aagtcgagca gggcggcgga aagggtatta
     5641 tcgccatcta acgggctaaa ccaagtcacc ggttgttcat cggtgatatc gagcgctacg
     5701 gcgagctccg ggtgaacgcg ctgaaacagg ctgatggcga cagaatcagc aatggtgtaa
     5761 tcgagttttc cttcgatgac atcttccatt aattccgcag agcctttttt gtcgtctacc
     5821 ttccagctta attccgggaa ttttgtgtct ttcagggttt ggagatcgtt aaccaccacg
     5881 tgacccggtg caacggtgag ttgctccgcc gtcaggttgc ccagtgtgcg cgggcgatac
     5941 tgactcactt tataaaccag ttgttgtgac acggaataat aggtagggcc aggctgataa
     6001 ttttttaccc gctcactgtt atagaccagt cctgccgcca gcaggtcggc gttaccatta
     6061 tcgaggtcgt caaacagctg gctgatattc tgccgcacgg tcactttcag ttttacaccg
     6121 aggtaatcgg caaactgttt cgccagttcg taatccaggc caaaaggttt cccgttgatt
     6181 tcgttataag tcaggggagt atgaatggtg ctcacacgca actctccccg cgcttgaatg
     6241 gcggcgatac ggttgtcggc tttaccaaac cagggaatgg atggccagag agcgaccgcg
     6301 agcagcagtg ccagaatgcc gatgaacaga taattaatct ttaatttttt caattagtta
     6361 attctctgtg tcgtgcgcgt cccagcttga aaaaacgtaa taatagtgaa aggtttactc
     6421 ataaatgagc ggcattttgc gtaaacctgc gccagatggc aacttattac agccattggc
     6481 ggcacgcgtt gctaattcac gatggtgatt ttatttccac gcaaacggtt tcgtcagcgc
     6541 atcagattct ttataatgac gcccgtttcc cccccttggg tacaccgaaa gcttagaaga
     6601 cgagagactt atgatggaaa ttctgcgtgg ttcgcctgca ctgtcggcat tccgaatcaa
     6661 caaactgctg gcacgttttc aggctgccag gctcccggtt cacaatattt acgccgagta
     6721 tgtccatttt gctgacctca atgcgccgtt aaacgatgat gagcacgcac aacttgaacg
     6781 cctgctgaaa tatggcccgg cactcgccag ccacgccccg caaggcaaac tcctgctggt
     6841 gaccccgcgt cctggcacca tctctccctg gtcttcgaaa gcgaccgata ttgcccataa
     6901 ctgcgggcta caacaggtaa accgccttga gcgcggcgtt gcttactata tagaagccgg
     6961 tacgctgacc aatgaacaat ggcagcaggt taccgctgaa ctgcacgacc gcatgatgga
     7021 aacggtcttt tttgctttag atgatgcaga gcagttgttt gcccaccatc aaccgactcc
     7081 ggttaccagc gttgatttgc tggggcaggg ccgtcaggcg ctgatcgacg ctaacctgcg
     7141 tcttggcctg gctctggcgg aagatgaaat tgactatctg caggatgctt tcacaaagct
     7201 tggtcgtaac ccgaacgaca tcgagctgta tatgttcgct caggcgaact ctgagcactg
     7261 ccgccacaaa atttttaatg ccgactggat tatcgacggt gaacagcagc cgaaatcgct
     7321 gttcaagatg atcaaaaata ctttcgaaac cacgccagat cacgttctct ctgcttataa
     7381 agataacgcc gccgtaatgg aaggttctga agtgggccgc tactttgctg accacgaaac
     7441 gggccgctac gatttccatc aggaaccggc gcatattctg atgaaagtcg aaactcacaa
     7501 ccacccgacg gcgatttctc cgtggccggg cgcggcgacg ggttccggcg gcgaaattcg
     7561 tgatgaaggc gccaccggac gcggtgcgaa gccgaaggct ggtctggttg gtttctccgt
     7621 ttccaacctg cgaattcctg gtttcgaaca gccgtgggaa gaagatttcg gtaagcctga
     7681 gcgcattgtc accgcgctgg acatcatgac cgaaggcccg ctgggcggcg cggcgtttaa
     7741 caacgaattt ggtcgtccgg cactgaatgg ctacttccgt acttatgaag aaaaagtgaa
     7801 cagccacaac ggcgaagagc tgcgcggtta tcacaaaccg atcatgctgg cgggcgggat
     7861 cggcaacatt cgcgccgatc acgtacaaaa aggcgagatc aacgtcggtg cgaagctggt
     7921 cgttctcggc ggcccggcaa tgaacatcgg tcttggcggc ggagcggcgt cttccatggc
     7981 gtcaggtcag tctgatgccg acctcgactt tgcttccgta cagcgcgaca acccggagat
     8041 ggagcgtcgc tgccaggaag tgatcgaccg ttgctggcag cttggtgatg ccaacccaat
     8101 cctgtttatc cacgacgtcg gcgctggcgg tctttctaac gcgatgccgg aactggtgag
     8161 cgacggcggg cgcagcggta aatttgaact gcgcgatatt ctaagcgacg aaccgggcat
     8221 gagcccgctg gaaatctggt gtaacgaatc ccaggaacgc tacgtgctgg cggttgctgc
     8281 cgatcaatta ccgctgtttg acgaactgtg taagcgtgag cgcgcaccct acgcggtgat
     8341 tggtgaagcg accgaagaac tgcatctttc tctgcacgat cgtcattttg ataatcagcc
     8401 gatcgatctg ccgctggacg tcctgcttgg taaaacgccg aagatgaccc gcgatgtaca
     8461 aacgctgaaa gcgaagggcg atgcgctggt ccgtgaaggg atcaccattg cagacgcggt
     8521 gaaacgcgta ctgcatctgc cgaccgtggc agagaaaacc ttcctggtga ccatcggcga
     8581 ccgcagcgta accggcatgg tagcgcgcga tcagatggtg gggccgtggc aggtgccggt
     8641 cgctaactgc gcggtcacta ccgccagcct cgacagctac tacggtgaag cgatggcgat
     8701 tggcgagcgt gcgccggttg cactgctgga tttcgccgcc tctgcccgtc tggcggtcgg
     8761 tgaagcgtta accaatattg ccgcaacaca gattggcgat atcaaacgca ttaaactttc
     8821 cgccaactgg atggcggcgg caggccaccc tggtgaagat gcgggcctgt atgaagccgt
     8881 taaagccgtg ggcgaagagc tttgtccggc gctgggcctg acgatcccgg tgggtaaaga
     8941 ctctatgtcg atgaaaaccc gctggcagga aggtaacgaa gagcgcgaaa tgacgtcgcc
     9001 gctgtcgctg gtgatttctg catttgcccg cgtggaagat gtgcgtcaca ccatcacgcc
     9061 acagctttct accgaagata acacactgct gctgattgat ttgggcaaag gcaataacgc
     9121 gctgggcgca acggcgctgg cgcaggttta tcgtcagctt ggcgacaaac cggcagatgt
     9181 acgcgacgtc gcgcaactga aaggcttcta tgacgcgatt caggcgctgg ttgcacagcg
     9241 taagctgctc gcataccacg accgctctga tggcggcctg ctggtaacgc tggcggaaat
     9301 ggcctttgct ggtcattgcg gcattaacgc ggatattgcc tctctgggcg acgatcgcct
     9361 ggcggcgttg tttaacgaag aactgggtgc ggtgattcag gttcgtgccg ctgaccgtga
     9421 agcggtcgag tccgtactgg cacagcatgg gcttgctgat tgtgtccatt atgtagggca
     9481 ggcggtttcc ggtgaccgtt ttgtgattac cgccaacggg cagactgtat tcagcgaaag
     9541 ccgcaccacg ttgcgtgtct ggtgggcaga aactacctgg cagatgcagc gcctgcgtga
     9601 caacccggag tgtgctgacc aggagcacca ggcgaaatca aacgacgccg atccgggcct
     9661 gaatgtgaaa ctgtcgttcg atatcaacga agatgtggca gcaccgtata ttgccactgg
     9721 cgcacgtccg aaagttgctg tgctgcgtga gcagggcgtg aactcgcatg ttgaaatggc
     9781 ggcagctttc caccgtgcag gctttgatgc tatcgacgtg catatgagtg acctgctgac
     9841 cggacgcacg ggcctggaag atttccacgc cctggtcgcg tgcggtggtt tctcctacgg
     9901 tgatgtgctg ggtgccggtg aaggttgggc gaagtcaatc ctgttcaatg accgtgtacg
     9961 cgatgagttt gcaaccttct tccaccgtcc gcaaacgctg gcgctggggg tatgtaacgg
    10021 ttgccagatg atgtctaacc tgcgtgaact gatcccgggg agcgagttgt ggccacgttt
    10081 tgtgcgtaac acctccgatc gctttgaagc gcgtttcagc ctggttgaag taacccaaag
    10141 cccgtctctg ctgttgcagg ggatggtggg ctcgcaaatg ccgattgctg tctctcatgg
    10201 tgaagggcgc gtggaagtgc gtgatgcggc gcatctggcg gcactggaaa gcaaagggct
    10261 ggtggcactg cgctatgtcg ataacttcgg caaagtcact gaaacctacc cggctaaccc
    10321 gaacggttca ccgaacggta ttacggcggt tacgactgaa agtggtcgag tcaccattat
    10381 gatgccgcac ccggaacgtg ttttccgtac tgtcagcaac tcctggcatc cggaaaactg
    10441 gggcgaggat ggcccatgga tgcgcatttt ccgcaatgcg cgtaagcagt tggggtaagt
    10501 catcagttca ttggttttgc agcccggatg cggcattaag caccaaccac tatcataagc
    10561 tcccaactcc gggagctttt ttgtgtctgt aaatcacgac aatgggtggt ttaccgtgtc
    10621 gctttctggc gacacttaaa tgattgattt tattgcaatc taataagttt atcttaagtt
    10681 gtctcttttt agcgacacag tggctgattt acattcaaat agcgaagaag ccataaatcc
    10741 ctcaaagcaa cgtataatca gtacgttacc aaactatttt ctttattggc acagttactg
    10801 cataatagta agcagtggct cattcaccga cttatgtcag ccccttcggg acgtgctaca
    10861 taaaattcga atgacgcaca acaaggtgcc tgccgtccaa cttctgatat cagcgtagct
    10921 atatcaacca tcgggcgaaa cgtcgagtta ggcaccgcct tattccataa caaagccggg
    10981 taattcccgg ctttgttgta tctgaacttc ccctcggtta gcatcaggct attcgcgtct
    11041 gacgagagta acaccttgaa acgctggccc gtttttcccc gctcattacg acaactggta
    11101 atgctggcat ttttgctaat tctactaccc ctgttggtgc tggcatggca agcctggcaa
    11161 agcctgaatg cgcttagcga tcaggcggcg ctggttaacc gcactacgct tattgatgcc
    11221 cggcgtagtg aagcgatgac caacgcggcg ctggagatgg agcgtagcta ccgtcagtat
    11281 tgcgtgctgg acgaccctac gcttgcgaag gtttatcaaa gccagcgcaa gcgttacagc
    11341 gaaatgctcg atgcccacgc aggcgtactg ccagacgata aactttacca ggcattgcgt
    11401 caggatttga acaatctggc ccaacttcag tgtaacaaca gtggtcccga tgctgccgcc
    11461 gccgcgcgtc ttgaagcctt tgccagtgcc aataccgaaa tggtgcaggc cacgcgcaca
    11521 gtggtgttct ctcgtgggca gcaacttcag cgtgaaatcg ccgaacgtgg gcaatatttt
    11581 ggttggcaat cgctggtgct atttctggtg agtctggtga tggtgctact tttcacgcgg
    11641 atgattatcg ggccggtgaa aaatatcgag cgcatgatca accgtctggg ggaagggcgt
    11701 tctctgggca atagcgtttc gttcagtgga ccgagcgagt tacgctcggt tgggcaacgt
    11761 attctttggt taagtgagcg cctgtcatgg ctggaatccc aacgccatca atttttaaga
    11821 catttatctc atgaattaaa aacgccactg gcgagtatgc gcgagggcac tgaattactg
    11881 gctgaccagg ttgtcgggcc gcttacgcca gagcaaaaag aggtggtgag cattcttgat
    11941 agcagcagcc gcaatttgca aaaactgatc gaacaactgc ttgattacaa ccgtaaacag
    12001 gcggacagtg cggtggaact ggagaatgtt gaattagcac cgctggtgga gacagtggtt
    12061 tctgctcata gcctgcccgc acgggctaaa atgatgcata ccgacgtcga tctcaaagca
    12121 acagcttgcc tggcggagcc aatgctgctg atgagcgtac tggataatct ttactccaat
    12181 gcggtgcact acggggctga atccggtaac atttgccttc gcagcagttc acatggtgcg
    12241 cgggtttata ttgatgtcat caatacaggc acgcccattc cgcaagagga acgcgccatg
    12301 atcttcgaac ccttttttca gggaagccac cagcgaaaag gggcggtgaa gggcagcggt
    12361 ctgggattaa gcattgccag ggattgtatt cgccgtatgc aaggggaact gtatctggtc
    12421 gacgagagcg ggcaagacgt ttgtttccgc attgaattac cgtcgtcgaa aaacacgaaa
    12481 taatgctaac agcctatcct atcaggctat tttatttgtc agttcggccc cgggcagtgc
    12541 tcgaaatctt cacgtacgat atgtacgctc cggtttctcc gcgctgtcca tgtccagact
    12601 gtctgcaaca attacgccca ctgggatagg ctttaagtct ggtgaatatg cgacacattt
    12661 ttcaacgatt attgccccga cggctgtggc tggcagggct gccatgtctg gcattgctgg
    12721 gttgcgtgca gaatcacaat aagccagcca ttgatacgcc agcagaagaa aaaattccgg
    12781 tttatcaact ggctgactat ctttctactg aatgtagcga catctgggcg ctgcaaggta
    12841 aatcaaccga aaccaatccg ctttactggc tgcgggcgat ggattgtgct gatcgtttaa
    12901 tgcctgcgca gtcgcgtcag caagctcgcc aatatgacga cggcagctgg caaaatacct
    12961 ttaaacaagg gattttgctc gccgacgcca aaattacgcc gtatgagcgc cgccagctgg
    13021 tcgcgcgtat tgaagcgtta agtaccgaga tcccggcaca ggttcgccca ctgtatcaac
    13081 tctggcgcga cggtcaggcg ctgcaactgc aactggcgga agagcgccaa cgttacagta
    13141 aactccagca gtccagcgac agcgagctgg atacattgcg ccagcaacat cacgttctac
    13201 agcagcaact ggaactcacc acccgcaagc tggaaaacct gaccgatatt gaacgccagc
    13261 tctcgacccg caaaccgact ggaaatttct cgccggatac gccgcacgag agtgaaaagc
    13321 ccgcgccatc cacccatgag gtcactcctg atgagccata aacctgcgca tttattattg
    13381 gtcgatgacg atccgggatt gctgaaactg cttggcctgc gcctgaccag cgaaggctac
    13441 agtgtggtca cggcggaaag tggcgctgaa ggattacggg tactgaatcg cgaaaaagta
    13501 gatttagtta tcagcgacct gcggatggat gaaatggacg gtatgcagct atttgctgaa
    13561 atccagaaag tgcagccggg aatgccagta attattctta ctgcgcatgg ttctatcccc
    13621 gatgccgttg ctgcaacaca gcagggcgtt tttagtttcc tcaccaagcc tgtcgataaa
    13681 gatgcgctat atcaggcaat tgacgatgcg ctggagcaat ccgcgccagc caccgatgaa
    13741 cgctggcgcg aggcaattgt cacccgcagc ccgctgatgc tgcgtttgct ggaacaggcg
    13801 cggctggtgg cgcaatcaga cgtcagcgtt ttgattaacg gtcagagcgg caccgggaaa
    13861 gagattttcg cccaggctat ccacaacgcc agcccgcgca acagcaaacc atttattgct
    13921 attaactgtg gcgcattgcc cgagcaattg ctggagtcgg agctgtttgg tcatgcgcgt
    13981 ggcgcgttta ctggcgctgt cagcaatcgc gaaggtttat tccaggcggc ggaaggcgga
    14041 acgctatttc tcgatgaaat tggcgatatg cctgcgccgt tacaggtcaa actgctgcgc
    14101 gtgttgcagg agcgtaaagt gcgcccgctg ggcagtaacc gcgatattga tatcaatgtg
    14161 cggattattt ctgccactca ccgtgatctg ccaaaagcga tggcgcgcgg ggaattccgc
    14221 gaagatctct attaccgcct caacgttgtc agcctgaaaa ttccggcact ggcggagcgc
    14281 acagaagaca ttccgctact ggcaaatcac ctgttgcgcc aggcggcaga gcgacataaa
    14341 ccgtttgtcc gcgcgttctc taccgatgcg atgaaacgcc tgatgaccgc gagctggccg
    14401 ggtaatgtgc gccagttggt caacgtgatt gaacagtgcg tggcgctgac ctcatctccg
    14461 gtgattagtg atgcgctggt ggagcaggcg ctggagggtg aaaatacggc gctgccaacc
    14521 tttgttgagg cacgtaatca gtttgaactc aactatttgc gtaagctgct gcaaatcacc
    14581 aaaggcaacg tcacccacgc ggcgagaatg gcggggcgca accggacaga attttataaa
    14641 ctgctttccc gacacgagct ggatgcaaac gatttcaagg aatgaattgg cgttatgtgt
    14701 tacgtttagc agatcaaaag acaggcgacc ttttcaagga atagcatgaa aaagattgat
    14761 gcgattataa aacccttcaa gctggacgat gtccgcgaag cactggccga agtcggtatt
    14821 accggcatga cggtgaccga agtgaaaggc tttggtcgcc agaaaggcca taccgagctg
    14881 taccgcggcg cggagtatat ggtggatttt ctgccgaaag tgaaaattga gattgtcgta
    14941 ccggacgaca ttgtcgatac ctgtgtcgat accattattc gcacggcgca aaccggcaaa
    15001 atcggtgacg gtaaaatctt cgtctttgac gtggcacggg tcattcgcat ccgtaccggt
    15061 gaagaggacg acgcggcaat ttaaaccgcg tcaagggttg ccggatgttt ccatccggca
    15121 acatcaaatt acagcacctt atgcgggcca aagcattcgt aatgaatgtt ttcctgcttc
    15181 acgcccagat ccactaactg tttcgcggta aactgcatga agccaaccgg gccgcataga
    15241 tagaactgca ttgtcggatc gctgaacgca ccttccagtt tgctcaaatc catcagacct
    15301 tcgctatcaa actgaccttt agcgcgatcg gcttcgctcg gctgacgatg ccaggtgtgc
    15361 gcggtaaagc gcggcagtga ctgccccagt tccttaactt catcggcaaa ggcgtgaaca
    15421 tcgccatttt ctgccgcatg gaaccagttc acttgtgctg tgtggcctgc ttttgccagc
    15481 gtgtcgagca ttgccagcat tggcgtttga ccaacaccgg cagagattaa cgtcactggt
    15541 gtgtcatctg cgacagccat aaagaaatca cctgccggag cgaccagttt cacgacatcg
    15601 ccaacattgg cgtgattgtg caaccagttg gatacctgcc caccctcttc gcgtttcacc
    15661 gcaatacgat agcctttgcc atccggtttg cgagtcaaag agtactgacg aatttcctga
    15721 tgcgggaaac cttccggctt cagccagacg ccgagatatt gccccggacg gtattctgcc
    15781 actgcgccac cgtcgaccgg ctccagttcg aagctggtga taagcgcgct gcgcggtgtt
    15841 ttagccacaa tgcggaaatc gcgagtacct tcccaaccac cggctttgct ggcgttttcg
    15901 ttatagattt ccgcctcgcg attgataaat acattagcca gtacaccata ggctttaccc
    15961 cacgcgtcca gcacttcctg ccccgggctg aacatttcgt ccagcgttgc caacaggtgt
    16021 tcaccgacga tgttgtactg ttccggtttg atctggaagc tggtgtgctt ctgcgcgatt
    16081 ttttctaccg ctggcagcag cgcaggcagg ttttcaatat tactggcgta ggcggcaata
    16141 gcgttaaaca gggcttcacg ttgatcgcca ttacgctggt tactcatgtt aaaaatttct
    16201 ttgagttctg ggttatgagt aaacatacgg tcgtagaaat gggcggttaa ctttggcccc
    16261 gtttccacca gtaaagggat ggtggctttt actgtagcga tggtttgagc gtcaagcata
    16321 tggtcttcct ttttttgcat cttaattgat gtatctcaaa tgcatcttat aaaaaatagc
    16381 cctgcaatgt aaatggttct ttggtgtttt tcagaaagaa tgtgatgaag tgaaaaattt
    16441 gcatcacaaa cctgaaaaga aatccgtttc cggttgcaag ctctttattc tccaaagcct
    16501 tgcgtagcct gaaggtaatc gtttgcgtaa attcctttgt caagacctgt tatcgcacaa
    16561 tgattcggtt atactgttcg ccgttgtcca acaggaccgc ctataaaggc caaaaatttt
    16621 attgttagct gagtcaggag atgcggatgt taaagcgtga aatgaacatt gccgattatg
    16681 atgccgaact gtggcaggct atggagcagg aaaaagtacg tcaggaagag cacatcgaac
    16741 tgatcgcctc cgaaaactac accagcccgc gcgtaatgca ggcgcagggt tctcagctga
    16801 ccaacaaata tgctgaaggt tatccgggca aacgctacta cggcggttgc gagtatgttg
    16861 atatcgttga acaactggcg atcgatcgtg cgaaagaact gttcggcgct gactacgcta
    16921 acgtccagcc gcactccggc tcccaggcta actttgcggt ctacaccgcg ctgctggaac
    16981 caggtgatac cgttctgggt atgaacctgg cgcatggcgg tcacctgact cacggttctc
    17041 cggttaactt ctccggtaaa ctgtacaaca tcgttcctta cggtatcgat gctaccggtc
    17101 atatcgacta cgccgatctg gaaaaacaag ccaaagaaca caagccgaaa atgattatcg
    17161 gtggtttctc tgcatattcc ggcgtggttg actgggcgaa aatgcgtgaa atcgctgaca
    17221 gcatcggtgc ttacctgttc gttgatatgg cgcacgttgc gggcctggtt gctgctggcg
    17281 tctacccgaa cccggttcct catgctcacg ttgttactac caccactcac aaaaccctgg
    17341 cgggtccgcg cggcggcctg atcctggcga aaggtggtag cgaagagctg tacaaaaaac
    17401 tgaactctgc cgttttccct ggtggtcagg gcggtccgtt gatgcacgta atcgccggta
    17461 aagcggttgc tctgaaagaa gcgatggagc ctgagttcaa aacttaccag cagcaggtcg
    17521 cgaaaaacgc taaagcgatg gtagaagtgt tcctcgagcg cggctacaaa gtggtttccg
    17581 gcggcactga taaccacctg ttcctggttg atctggttga taaaaacctg accggtaaag
    17641 aagcagacgc cgctctgggc cgtgctaaca tcaccgtcaa caaaaacagc gtaccgaacg
    17701 atccgaagag cccgtttgtg acctccggta ttcgcgtggg tactccggcg attacgcgtc
    17761 gcggcttcaa agaagcagaa gcgaaagaac tggctggctg gatgtgtgac gtgctggaca
    17821 gcatcaatga tgaagccgtt atcgagcgca tcaaaggtaa agttctcgac atctgcgcac
    17881 gtttcccggt ttacgcataa gcgaaacggt gatttgctgt caatgtgctc gttgttcatg
    17941 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaactt tgcaaattca atatattgca
    18001 atatccgtgt aggcctgata agcgtagcgc atcaggcaat cttaggtatg tgatcatcaa
    18061 ggcttccttc gggaagcctt tctacgttat tgcgccatca aatctgtcgt aactgcgcct
    18121 caacatacaa atagccaatc cccaacacct gttgtgcgcg gcttaattgc ccaaagccaa
    18181 tttgcgttgc tttcacgctc ggcccttcgt cgcggtcgaa cgggttaaat cccgtctggc
    18241 gaataatggt attaagccag tgctcaccaa aggcacaact gcgaccgtac aaccaaatct
    18301 gattaatatt gaggatgttg aggaagttat acagactcaa accaatggca ttggcactgc
    18361 gatcaaccca actggtgatc cacggttctc cactctacca ggcagcgatt aactgcgctg
    18421 tagtcagttt ttccggatca agctgagtat taaccggttg tgattttagc catacccgcg
    18481 cctgtttttt taatgcactt aacgaggcga cggtttccag gcagccgtaa cgtccacagt
    18541 cgcagacgac gccatccgga ttaacgatgg tgtgactaat ctgtccgcta ccatacaaac
    18601 tgccgcgata aatttgctcg ttaatcacga acgacgagcc aatgccgtaa tcaacgttga
    18661 tcacgcagaa atcccgtttc tgcgaattat tttgccattt ctccgccagc gccagcatca
    18721 cgcagtcatt atcgaccatc acccgaatgc cgagcttctc ttccagcaga tacttcacct
    18781 caaccggcgt tgtccacggc gcttgcggca tggtttgcga cacgccggtc accggatcaa
    18841 cctgaccgtg gattgccagc gccagattga tggtgcggtc cggccacaat ttacggtggc
    18901 gatgccagca tttttcgatt tcggacagca gcgcctgcgg cgtcggtgca tcaatctgta
    18961 aatactcaaa ttcacctttc ggacttaaac aagcattagc aacctgacac tcaatactgg
    19021 tgggcgtcac gttcaggcac agcgtccagt caccttccgg cgcaatcagc catgtaccgc
    19081 tactatgccc gcgcgtctgg ctttcatcgt cgatattcac cacccgtttt tcgctttcca
    19141 gttcctgcaa aatattactg actgccggaa tcgaaatttg cgccagccgg gccagcgttg
    19201 atttattggc gcgcttttgc cggtacagca gttccagaat cacgcatttg ttatggtggc
    19261 gaatctgttg attgttaatg caggctctca tcttcactaa accttgttta gttaattgcg
    19321 tgattattcc gcgaaaacat tgccagattg ttcaatacac tgccacaaat cttttaattc
    19381 agtatgtctt gttaatattg agggcaccat gactccagta aaagtgtggc aagagcgcgt
    19441 tgagatcccg acctatgaaa ccgggccgca ggatatacat cccatgttcc tggaaaatcg
    19501 cgtttatcag ggatcgtccg gcgcggttta tccctacggc gtgaccgata cgctgagcga
    19561 gcagaaaacc ctgaaatcct ggcaggcggt gtggctggaa aacgactaca tcaaagtgat
    19621 gatcctgccg gaactgggcg gtcgggtgca tcgcgcgtgg gataaagtga aacagcgcga
    19681 ttttgtttat cacaatgaag tcattaaacc tgcgctggtg gggctgctgg ggccgtggat
    19741 ttccggtggg attgagttta actggccgca acatcatcgc ccgaccacct ttatgcccgt
    19801 tgatttcacc ctcgaagccc atgaagacgg tgcacagacg gtgtgggtag gcgaaacgga
    19861 gccgatgcat ggtttacagg tgatgacagg tttcaccctg cgccctgggc ggcgctggaa
    19921 atcgccagcc gcgtctataa cggcaacgcc acgccgcgtc atttcttgtg gtgggccaac
    19981 ccggcagtga aagggggtga agggcatcag agcgttttcc caccggattt gactgcggtg
    20041 tttgatcacg gcaaacgggc cgtctccgct ttccccatcg ctaccggcac ttactacaaa
    20101 gtggactact ccgctggagt ggacatttct cgctataaaa atgtgcccgt tccaacctca
    20161 tatatggccg aaaaatcgca gtacgatttt gtcggcgcgt ggtgtcacga tgaagatggc
    20221 ggtttgctgc acgttgccaa ccaccatatt gcgccaggta aaaaacagtg gagttgggga
    20281 cacagtgaat ttggccaggc gtgggataag agtctgaccg acaataacgg cccgtatatc
    20341 gaactgatga ccggtatttt tgccgataac cagcccgatt ttacctggct tgatgcttac
    20401 gaagagaagc gttttgagca gtatttcctg ccttatcatt ctctgggcat ggtgcaaaat
    20461 gcctcccgcg atgcggtgat aaaactccag cgtagtgagc aggggattga gtgggggctg
    20521 tatgccatct ctccgttgaa cggataccgc ctggcgatcc acgaaatcgg caaatgcaac
    20581 gcgttacttg atgatgccgt ggcactgatg cctgcgaccg ccatccaggg cgtgttgcac
    20641 ggtatcaatc ctgataggct gaccattgag ctttccgatg ccgacggcaa tattgtactg
    20701 agttatcagg aacatcagcc gcaagcgttg ccgttaccgg acgtcgccaa agcgccactg
    20761 gcagcacaag acattaccag tacagatgaa gcctggttta tcggtcagca tctggagcaa
    20821 tatcatcacg ccagccgttc accgttcgat tactacctgc gcggcgtggc gctggacccg
    20881 ctggattatc gctgtaacct ggcgctggcg atgctggaat ataaccgcgc agatttcccg
    20941 caagcggtgg cgtatgccag tcaggcgctg aaacgcgcac atgcgctgaa caaaaacccg
    21001 cagtgtggac aagcaagttt gattcgcgcc agtgcttacg aacgtcaggg acaatatcaa
    21061 caagccgaag aggatttctg gcgtgcggtc tggagcggca acagcaaagc cggtggctat
    21121 tatggcctgg cacgactggc ggcgcgtaat ggtaacttcg acgctggtct ggatttttgc
    21181 caacaaagtc ttcgcgcctg cccaaccaat caggaagtgc tttgcctgca taatctgctg
    21241 ctggtgttaa gtggtcgtca ggacaacgcg cgtttgcagc gcgcgaaact gctgcgtgat
    21301 tatccgctga acgccattct gtggtggctg aactggttcg atggtcgtag cgaatcagcc
    21361 ctcgcgcagt ggcgcggtct gtgtcaggga cgcgacgtta acgccctgat gaccgccggg
    21421 caactgatta actggggaat gcccactctg gcgacagaga tgctgaacgc actggactgt
    21481 cagcgcacgt tatcgcttta tctacaagcc agcttgctgc cgaaagccga acgcggcgaa
    21541 ctggtcgcaa aagccattga tgtcttcccg cagtttgtcc gtttcccgaa tacgctggaa
    21601 gaagtggcgg cgctggagag tattgaagag tgctggtttg cccgtcattt actggcctgt
    21661 ttctactaca acaagcgcag ctacaacaaa gccattactt tatggcaacg ttgcgtagag
    21721 atgtcgccgg agtttgccga tggctggcgc gggttggcga tccatgcgtg gaataagcaa
    21781 cacgattatg aactggccgc gcgttatctt gataatgctt atcagcttgc gccgcaggat
    21841 gcacgtctgc ttttcgaacg ggatttgctg gataagttaa gtggagccac accggagaaa
    21901 cgactggcgc gtctggaaaa taatctggaa attgcgctga aacgcgacga catgaccgca
    21961 gaactgctca atttgtggca tctcacgggt caggcagaca aagcggcgga cattctcgcc
    22021 acgcgcaaat tccacccgtg ggaaggcggg gaagggaagg tcaccagtca gtttatcctc
    22081 aaccagttat tacgcgcctg gcagcatctt gatgccagac agccgcagca ggcctgcgaa
    22141 ctgcttcatg ccgcgctgca ttatccggag aatttaagcg aaggccgttt accggggcaa
    22201 actgataacg acatctggtt ctggcaggcg atatgcgcca acgcgcaggg cgatgaaact
    22261 gaagcgacgc gttgtttacg tctggcggcg accggcgatc gcaccattaa catccacagt
    22321 tattacaacg atcagccggt tgattatctc ttctggcaag gaatggcgct gcgactgctg
    22381 ggcgaacaac aaatcgcaca gcaactgttt agtgaaatga aacagtgggc gcaagagatg
    22441 gcgaaaacca gtattgaggc ggatttcttt gctgtttcac aacctgacct gttgtcgctg
    22501 tatggcgatt tacaacagca gcataaagaa aaatgcctga tggtggcgat gctggcgtcc
    22561 gcgggactcg gggaggttgc gcaatatgaa tctgctcgcg ctgaattgac ggcgattaat
    22621 ccggcctggc cgaaagcggc attattcacc accgtgatgc cttttatttt taactacgtt
    22681 cactaaaccc tattaactgt aatatttgag cggcacgcgc tgccgctcat cacaaaataa
    22741 ttagaataag ccgttgttaa taaaaactct acaggaggca ctatgcctaa aaaaatgaga
    22801 acaacccgta atctattatt aatggctacg ctattaggta gcgcactatt tgccagggct
    22861 gcggataaag aaatgaccat tggtgcaata taccttgata ccctgggata ttacgctgga
    22921 gtgcgtcagg gcgttcagga tgcggcaaaa gattcttcag tacaggtaca gttaattgaa
    22981 actaacgccc agggtgatat ttcgaaagaa agtacctttg ttgataccct cgtggcgcgt
    23041 aatgtcgatg ccattatttt atcggcagtg tctgaaaatg gcagtagccg taccgttcgt
    23101 cgcgccagtg aagcgggtat tccggtgatt tgctacaaca cctgtattaa tcaaaagggt
    23161 gtcgataaat atatctcggc gtatctggtc ggcgatccac tggaatttgg taaaaaactg
    23221 ggtaacgctg ccgccgatta ttttattgcc aataaaattg accagccgaa aattgccgtc
    23281 atcaattgcg aagcctttga agtttgtgtg cagcgacgta aaggatttga agaagtatta
    23341 aaatcccgcg ttcccggcgc gcaaattgtc gctaatcagg aagggactgt tttaaataaa
    23401 gcgatttccg ttggtgaaaa actgattatc tccacgccgg atctcaacgc cattatgggg
    23461 gagtcaggcg gtgcgacact cggcgcggta aaagcggtac gtaatcaaaa tcaggccgga
    23521 aaaattgctg ttttcggttc ggatatgaca accgaaattg ctcaggagct ggaaaacaat
    23581 caggtgctga aagcggtagt ggatatttcc ggtaagaaaa tgggcaatgc tgttttcgcg
    23641 caaacattga aggttatcaa taaacaagcc gacggtgaaa aagtgattca ggtgccaatc
    23701 gatctctata ccaaaacgga agatggtaaa cagtggctgg caacgcacgt tgatggtctg
    23761 ccctaatcgc tgattcgcag aggtccctat gttcacggca acagaggcag tcccggtagc
    23821 aaaagtggtg gcaggaaata agcgttaccc cggcgtcgtt gcgctggata acgttaactt
    23881 cacgctcaat aaaggcgaag ttcgtgcgct gttaggtaaa aacggcgcgg gcaaatcgac
    23941 tctcattcga atgcttaccg gcagcgaacg cccggatagc ggtgatatct ggattggcga
    24001 gacgcgactg gaaggtgacg aagctacgtt gactcgccgt gccgctgaac tgggggttcg
    24061 tgcggtttat caggaattaa gtctggtgga agggctgacg gtggcagaaa acctctgcct
    24121 tggtcaatgg cctcgccgca acggcatgat tgattacctg caaatggcgc aggatgccca
    24181 acgttgctta caggcgctgg gcgttgacgt tagtcctgaa caacttgttt caacgctaag
    24241 cccggcgcaa aagcaactgg tggaaattgc acgggtgatg aagggcgagc cgcgcgtggt
    24301 cattcttgat gaacccacca gctcgcttgc cagtgcagaa gttgaactgg tgatcagtgc
    24361 ggtgaaaaag atgtcggcac tgggcgtggc ggtgatttat gtcagccacc ggatggaaga
    24421 aatccgccgc attgcctcct gtgccaccgt tatgcgcgat ggtcaggtgg cgggcgatgt
    24481 gatgctcgaa aacacctcca cgcatcatat tgtgtcgcta atgctagggc gtgatcacgt
    24541 tgatattgcg ccggttgccc ctcaggaaat tgtggatcag gccgtactgg aagtccgtgc
    24601 gttacgccat aagcccaagc tggaggatat cagctttacg ttacgtcgcg gcgaagtgct
    24661 tggcattgct ggcctgctgg gggcagggcg cagtgaattg ctgaaggcga ttgttgggct
    24721 ggagacgtat gaacagggcg aaattgttat caacggcgag aaaatcacgc gccccgatta
    24781 cggcgacatg ctgaaacgcg gcattggcta tacgccagaa aaccgcaaag aagcggggat
    24841 cattccctgg ttgggcgttg acgaaaatac agtgctgacc aatcggcaaa aaatcagcgc
    24901 caacggagtg ctgcaatggt ccaccatccg ccgcctgacc gaagaggtga tgcagcggat
    24961 gacggtcaag gccgctagta gcgaaacgcc catcggcacg ctttctggtg gcaatcagca
    25021 aaaagtggtg atcggtcgtt gggtctatgc cgccagccag attttgttgc tcgacgagcc
    25081 aacgcgcggc gtcgatatcg aagccaaaca gcagatttac cgtattgtcc gtgagctggc
    25141 tgccgaagga aaaagcgtgg tgtttatctc cagtgaagtg gaggagttgc cgctggtgtg
    25201 tgaccgcatc ctgttattac agcacggcac gttctcgcag gagtttcact cgccggtcaa
    25261 tgtggatgag ctgatgtccg ccattctgtc tgtgcactga tgaattttac cgaggaacct
    25321 gaagatgtct gcttcgtcat taccattgcc gcagggcaag agcgtctcgc tcaaacaatt
    25381 tgtcagtcgc catattaatg agatcggttt gctggtggtg attgccattc tttatctggt
    25441 cttctccctg aacgcgcctg gctttatctc attgaataac cagatgaacg tgctgcgcga
    25501 tgccgccacc attgggattg ccgcctgggc gatgacgctg attattatct ccggtgaaat
    25561 tgatgtcagc gttgggccga tggtggcttt tgtctcggtg tgcctggcat ttttgctgca
    25621 atttgaagtt ccgttggcga ttgcttgtct gctggtgttg ctgttaggcg cgctgatggg
    25681 aacgctcgcc ggggtgctgc gcggcgtgtt taacgtacca agtttcgttg ccacactggg
    25741 gctgtggagc gccctgcgcg gaatggggct gtttatgacc aacgcactgc cagtgccgat
    25801 tgacgaaaac gaggtgctgg actggctggg cggacaattt ctcggtgtgc cagtatccgc
    25861 gctgatcatg atggtgttgt ttgcgctgtt tgtgttcatt agccgaaaaa ccgccttcgg
    25921 gcgctcggtt tttgccgttg gcggtaatgc cacggcggcg cagttgtgcg gtattaacgt
    25981 tcgtcgggtg cgcattctta tctttaccct ttcgggatta ttagcggcgg tgaccggcat
    26041 tttgctggcg gcacgcctcg gttccggtaa cgcaggtgcc gcaaacggtc tggagtttga
    26101 cgtcatcgcc gcggtcgtgg tcggcggtac ggcactttcc ggcggtcgcg gttccctgtt
    26161 cggtacattg cttggtgtgc tggtgattac gctaatcggt aacggtctgg tgctgctcgg
    26221 tattaactcc tttttccagc aggtggtacg cggcgtcatc atcgtggtgg cggtgctggc
    26281 gaatatcttg ctgacccagc gaagcagtaa agcgaaacgc taaaccttaa cgcccctggc
    26341 gcgggtgcgg cagggatgaa aattaatcgt attgttaatg agggatttat gaaaacgatg
    26401 ctggcagctt atttaccagg aaattcaacc gtcgatctgc gggaagttgc ggtgccgacg
    26461 ccggggatta accaggtact gatcaaaatg aaatcctccg ggatttgcgg aagcgatgtc
    26521 cactatatct atcatcaaca ccgtgccacg gctgcggcac ccgataaacc gttataccag
    26581 ggatttatca acggtcatga accgtgcggg cagattgtgg cgatggggca aggctgccgc
    26641 cattttaaag agggcgaccg cgtgctggtg tatcacattt ctggctgtgg tttttgcccg
    26701 aactgccgcc gcggctttcc tatctcttgt actggcaaag gaaaagcggc ttacggctgg
    26761 cagcgtgacg gcggtcatgc cgaatatctg ctggcggaag aaaaagatct gatcctcctg
    26821 ccggatgcgc tgagctacga agatggtgcg tttatcagtt gcggcgttgg cacggcctat
    26881 gaagggattt tgcgcggcga agtttccggc agcgataacg tgctggtggt cggtctgggg
    26941 ccggtcggca tgatggcgat gatgctggcg aaaggtcgcg gtgcaaaacg gatcatcggc
    27001 gttgatatgc tgccggaacg tctggcgatg gcaaaacagt taggggtgat ggatcacggc
    27061 tatttagcaa ccaccgaagg tctgccgcag attatcgccg aactcaccca cggtggcgcg
    27121 gatgttgcgc tcgattgttc tggtaatgcc gcaggtcgct tgctggcact gcaatccacc
    27181 gctgactggg gacgggtggt ttacattggt gaaaccggaa aagtggaatt cgaggtcagc
    27241 gccgatctga tgcaccatca acggcggatt atcggctcct gggtgaccag tctgttccat
    27301 atggaaaaat gcgcccacga tttgactgac tggaagctgt ggccgcgtaa cgccattacc
    27361 catcgcttct cgctggaaca ggcaggtgat gcctatgcgc tgatggcgag cggcaaatgc
    27421 gggaaagttg tgattaactt cccggattaa ggagttagcg gatgaccatc tataccttat
    27481 cccacgggtc gctaaagctg gacgtttctg accagggcgg cgtaatcgaa ggcttctggc
    27541 gcgatacgac gccgctctta cgtcctggta aaaaaagcgg tgtggcaacc gatgcctcat
    27601 gttttccgct ggtgccattt gcaaaccggg tgagcggcaa ccgttttgtc tggcaggggc
    27661 gtgagtatca actgcaaccg aatgttgagt gggatgcgca ctatctacac ggcgatggct
    27721 ggctgggcga gtggcaatgt gtctcgcaca gcgatgatag tttgtgtctg gtgtatgaac
    27781 atcgcagcgg tgtctatcac tatcgggtaa gtcaggcgtt tcatttaacg gcggatacgc
    27841 tgacggtgac gctctctgtc accaatcaag gggcagagac gctgccattt ggtaccggct
    27901 ggcatcctta ttttccgttg tcaccacaaa cgcggattca ggcgcaggcg agcggttact
    27961 ggctggaacg ggagcagtgg ctggcgggtg agttttgcga gcagctaccg caggaactgg
    28021 attttaacca gctcgcgccg ttgccgcgcc agtgggtgaa caatggtttt gccggatgga
    28081 atggtcaggc ccgcatcgag cagccgcagg aaggttatgc catcatcatg gaaacgacgc
    28141 cacctgcacc gtgttatttc atctttgttt ccgaccctgc gtttgataaa ggatatgcgt
    28201 ttgatttctt ctgtctggaa ccgatgagcc atgcgccgga tgatcatcat cgcccggaag
    28261 ggggtgatct cattgcgcta gcaccagggg aatcaacaac ttcagagatg tcgttgcggg
    28321 tagagtggct gtaatttatt aaatgccgga taaggcgttc acgccgcatc cggcatcttt
    28381 cctaacgccg atcgagagaa attgcccccg gcccggtaat tgccaatagc aagaacgcgc
    28441 cagcgatact gacattcttc cagaaattaa tcatatttgg cccaaccgca tcgccggtca
    28501 tatcccagta atggtgacca atcaccgccg tacccagcgt gtagaaaata aacagcaccg
    28561 ccagcggacg ggtgaaaaag ccaagcacga ttaatatcgc ggcgggcact tccataacta
    28621 ccgcaataat cgctgccagc atcggcattg gcgcgcccaa cgaggccata tattggaccg
    28681 taccgtcaaa gcccatcatt ttgggaaaac caaaaataat gaaaattaag accacggcga
    28741 tacgggcaat taataacaaa acggggcggg cagctccaaa atcaaaataa cgt
//
LOCUS       DAJTYZ010000043        28783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000043 DAJTYZ010000000
VERSION     DAJTYZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28783
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.043"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..205)
                     /gene="tuf"
                     /locus_tag="OO596_001793"
     CDS             complement(<1..205)
                     /gene="tuf"
                     /locus_tag="OO596_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6717660.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHV"
     gene            complement(276..2390)
                     /gene="fusA"
                     /locus_tag="OO596_001794"
     CDS             complement(276..2390)
                     /gene="fusA"
                     /locus_tag="OO596_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6717661.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2487..2957)
                     /gene="rpsG"
                     /locus_tag="OO596_001795"
     CDS             complement(2487..2957)
                     /gene="rpsG"
                     /locus_tag="OO596_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6717662.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3054..3428)
                     /gene="rpsL"
                     /locus_tag="OO596_001796"
     CDS             complement(3054..3428)
                     /gene="rpsL"
                     /locus_tag="OO596_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6717663.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3554..3841)
                     /gene="tusB"
                     /locus_tag="OO596_001797"
     CDS             complement(3554..3841)
                     /gene="tusB"
                     /locus_tag="OO596_001797"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6717664.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3849..4208)
                     /gene="tusC"
                     /locus_tag="OO596_001798"
     CDS             complement(3849..4208)
                     /gene="tusC"
                     /locus_tag="OO596_001798"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6717665.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4208..4594)
                     /gene="tusD"
                     /locus_tag="OO596_001799"
     CDS             complement(4208..4594)
                     /gene="tusD"
                     /locus_tag="OO596_001799"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6717666.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4594..5316)
                     /locus_tag="OO596_001800"
     CDS             complement(4594..5316)
                     /locus_tag="OO596_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6717667.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5483..6295)
                     /gene="fkpA"
                     /locus_tag="OO596_001801"
     CDS             complement(5483..6295)
                     /gene="fkpA"
                     /locus_tag="OO596_001801"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6717668.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6516..6734
                     /locus_tag="OO596_001802"
     CDS             6516..6734
                     /locus_tag="OO596_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6717669.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6783..7373)
                     /gene="slyD"
                     /locus_tag="OO596_001803"
     CDS             complement(6783..7373)
                     /gene="slyD"
                     /locus_tag="OO596_001803"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6717670.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7468..7668)
                     /locus_tag="OO596_001804"
     CDS             complement(7468..7668)
                     /locus_tag="OO596_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6717671.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7678..9483)
                     /gene="kefB"
                     /locus_tag="OO596_001805"
     CDS             complement(7678..9483)
                     /gene="kefB"
                     /locus_tag="OO596_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR6717672.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9483..10034)
                     /gene="kefG"
                     /locus_tag="OO596_001806"
     CDS             complement(9483..10034)
                     /gene="kefG"
                     /locus_tag="OO596_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6717673.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10165..12078
                     /locus_tag="OO596_001807"
     CDS             10165..12078
                     /locus_tag="OO596_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6717674.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12078..13100
                     /locus_tag="OO596_001808"
     CDS             12078..13100
                     /locus_tag="OO596_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6717675.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13094..13312
                     /locus_tag="OO596_001809"
     CDS             13094..13312
                     /locus_tag="OO596_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6717676.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13366..14235
                     /locus_tag="OO596_001810"
     CDS             13366..14235
                     /locus_tag="OO596_001810"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6717677.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14290..14694)
                     /locus_tag="OO596_001811"
     CDS             complement(14290..14694)
                     /locus_tag="OO596_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6717678.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14996..15628
                     /gene="crp"
                     /locus_tag="OO596_001812"
     CDS             14996..15628
                     /gene="crp"
                     /locus_tag="OO596_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6717679.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15679..17769
                     /locus_tag="OO596_001813"
     CDS             15679..17769
                     /locus_tag="OO596_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6717680.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17836..19056)
                     /gene="argD/dapC"
                     /locus_tag="OO596_001814"
     CDS             complement(17836..19056)
                     /gene="argD/dapC"
                     /locus_tag="OO596_001814"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6717681.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19142..19705)
                     /gene="pabA"
                     /locus_tag="OO596_001815"
     CDS             complement(19142..19705)
                     /gene="pabA"
                     /locus_tag="OO596_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6717682.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19737..20339)
                     /locus_tag="OO596_001816"
     CDS             complement(19737..20339)
                     /locus_tag="OO596_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6717683.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20329..20496)
                     /locus_tag="OO596_001817"
     CDS             complement(20329..20496)
                     /locus_tag="OO596_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6717684.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20601..21173)
                     /gene="ppiA"
                     /locus_tag="OO596_001818"
                     /gene_synonym="rotA"
     CDS             complement(20601..21173)
                     /gene="ppiA"
                     /locus_tag="OO596_001818"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6717685.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21444..22625
                     /gene="tsgA"
                     /locus_tag="OO596_001819"
     CDS             21444..22625
                     /gene="tsgA"
                     /locus_tag="OO596_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6717686.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22887..25430
                     /gene="nirB"
                     /locus_tag="OO596_001820"
     CDS             22887..25430
                     /gene="nirB"
                     /locus_tag="OO596_001820"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6717687.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25427..25753
                     /gene="nirD"
                     /locus_tag="OO596_001821"
     CDS             25427..25753
                     /gene="nirD"
                     /locus_tag="OO596_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6717688.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25879..26685
                     /gene="nirC"
                     /locus_tag="OO596_001822"
     CDS             25879..26685
                     /gene="nirC"
                     /locus_tag="OO596_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6717689.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26704..28077
                     /gene="cobA"
                     /locus_tag="OO596_001823"
     CDS             26704..28077
                     /gene="cobA"
                     /locus_tag="OO596_001823"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6717690.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28324..28491
                     /locus_tag="OO596_001824"
     CDS             28324..28491
                     /locus_tag="OO596_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6717691.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         6992 a         7708 c         7217 g         6866 t
ORIGIN      
        1 caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg ttatcgatct
       61 ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg attgcagcgg
      121 tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg tgcggttttg
      181 tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc tccccttcag
      241 gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc ttcaattacg
      301 gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt gtatgatgca
      361 cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga cagcggtact
      421 tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat accacgacga
      481 cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac ttcaaccttc
      541 atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa ggcgatagaa
      601 gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc gaagtgcaga
      661 cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag ctgttcctgg
      721 atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt aatgtcgttg
      781 atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc gataacaaca
      841 tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac atcggtaact
      901 ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc ttcaacgttg
      961 aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat acccgcgatg
     1021 atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc tttagccaga
     1081 cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc aactgcgatg
     1141 gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc cgggtcacac
     1201 agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc gcccgcgcga
     1261 acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc gaaacgctca
     1321 cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc ggagtaaaca
     1381 cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc cagtgcagag
     1441 aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc gtccaggata
     1501 ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc atccagcatc
     1561 gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat ttcgttgttc
     1621 agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc accacccagg
     1681 tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt ctggtgccat
     1741 tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt tacgccctgg
     1801 tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc ggtgaaatgt
     1861 tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg ggttttgatc
     1921 tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac gaacgcaatg
     1981 cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg ctgaacacca
     2041 ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg ttctacttcg
     2101 attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg ctcatactgc
     2161 ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat accacgttcc
     2221 tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc gattttatgg
     2281 tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc gtcgatgtgc
     2341 gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat ttgtttcctc
     2401 gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact ggaagcgccc
     2461 gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc cttgttggct
     2521 tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt ttctgcagca
     2581 tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt acgagcagct
     2641 tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc aactggtacc
     2701 tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg cacgttttcg
     2761 agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag ggtctccagc
     2821 gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag gatatttaca
     2881 aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg ctgaccaatg
     2941 acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca aaactctacg
     3001 agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa ccattaagcc
     3061 ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac gccggagcag
     3121 tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac acgaccgcca
     3181 cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat gtaggaagtc
     3241 acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga gttcggtttt
     3301 ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca tgcttccagc
     3361 gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag ctggttaact
     3421 gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa taaaacgtcc
     3481 tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca agaaatatac
     3541 aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt ctgacgaaat
     3601 cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg cgggcaataa
     3661 ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca aggtagcggt
     3721 taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg ccttcactga
     3781 gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat gtgtgcagca
     3841 taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg taaggcatct
     3901 gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc gcgttcgcgc
     3961 agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa tttaaaagtg
     4021 gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag ctgaaaaacg
     4081 ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc cagtaaagca
     4141 tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac aaacgcaatt
     4201 cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc agcgcgccaa
     4261 gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc cttccggcct
     4321 ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc agcgccacac
     4381 catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg cttgccggtg
     4441 aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg cttaactcat
     4501 ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt tgcgtaccgt
     4561 atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct ggaaatcacc
     4621 gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca ggcggtcagc
     4681 aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat tcagcacgat
     4741 ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt cgatggtgaa
     4801 ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc ttgaaccgac
     4861 atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa catcaagatt
     4921 catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg cgatagtcag
     4981 ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa cgctgctatc
     5041 cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg aaccaatctt
     5101 ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat cctgcaaaga
     5161 gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca ccaccgcttc
     5221 gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca gtaaatccaa
     5281 ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc cttttcagga
     5341 gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa attaatacag
     5401 cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa ccgccgcctg
     5461 gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc gcatcagctt
     5521 ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg tcaaacacca
     5581 gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt tctggtggaa
     5641 taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc caccccggga
     5701 taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg tcgaactctt
     5761 taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct ttcggtgctt
     5821 cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt ttcacacctt
     5881 tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca gccgcgtctt
     5941 tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc agagtctgtt
     6001 cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca ccagcgatca
     6061 gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag ttttccatgt
     6121 aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt ttgaacgctg
     6181 ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg attggtgcat
     6241 gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat ttcatccata
     6301 tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt actataaagc
     6361 gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca gactcttttt
     6421 gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa agttaagtag
     6481 aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca ttggaagcac
     6541 gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa gaactgaacg
     6601 tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg cgtctgctga
     6661 ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa gaaacgccac
     6721 cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc accttttcgg
     6781 tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc gccaccgtgt
     6841 tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg atcgtggtga
     6901 tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc ttcacgaatc
     6961 gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt accatcaacc
     7021 actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg gtcggtttca
     7081 gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc tttaggaaca
     7141 cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac agcgacatca
     7201 aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat cagggaaccg
     7261 tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa cacaccgtct
     7321 tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt catgatatct
     7381 cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc gtgtactcac
     7441 tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga tcacttgctc
     7501 atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat gtccgcactt
     7561 aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat cctgtgcctg
     7621 acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct ttaccctcta
     7681 ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac gctggaaaat
     7741 ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat cggcatgcat
     7801 tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt gcgcccgctg
     7861 cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct ctaacgcact
     7921 ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat gcgcttccac
     7981 acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc atatttccac
     8041 cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag cggcctctgc
     8101 acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt tgtagccata
     8161 tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc gcattttatt
     8221 tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga caatgacctg
     8281 gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt taaactggcg
     8341 ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg aaagcgtcac
     8401 cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag aagcggtaga
     8461 aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact gcatccgctc
     8521 tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt tcaccgccac
     8581 cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc cgaggttgag
     8641 cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg gatcgatagc
     8701 cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa taaatgtacc
     8761 gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca ccagcagcag
     8821 cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga ataccggacg
     8881 cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca tgccgatctt
     8941 catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg ccagtgctgg
     9001 gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg attcgctgcg
     9061 attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca tcgcaaggcc
     9121 aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata atcccgccag
     9181 caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac gccgcagttg
     9241 ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata ccacgccgag
     9301 ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc acgggccaat
     9361 tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg atgccaacgg
     9421 caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat cggaaccttc
     9481 catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc tctggcgtgg
     9541 ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg acttaaccaa
     9601 tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct catcgggtag
     9661 cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat cacgttacgc
     9721 cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg acttaatacc
     9781 cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg atgctgaaag
     9841 acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat aaaaaaatcg
     9901 ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc cggtttaagc
     9961 agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag caaaactttc
    10021 gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc tgttttgggc
    10081 taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat gataagttaa
    10141 catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat tcgtcgcggc
    10201 gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa agtcggcctg
    10261 gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa tgaaatcagc
    10321 gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt gaatcaggaa
    10381 acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg tgaatatcgt
    10441 caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc cattgcgacc
    10501 attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc cgccagcctg
    10561 ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga tttctccggt
    10621 ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga cttgctgctg
    10681 ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga aaaatggttg
    10741 aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct cgatccgatc
    10801 gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg caactacagt
    10861 tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta tgaaagccag
    10921 caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa agccaccaaa
    10981 gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat tgcccccgcg
    11041 cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc aaatccgtta
    11101 ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga ctcgattaaa
    11161 ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc gggtaaatcg
    11221 acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat tggtctggcg
    11281 aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg cgccgacgaa
    11341 tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa actgcgtgac
    11401 tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg ccgcttctcc
    11461 ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc gaatctgctg
    11521 ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact caccgaagca
    11581 ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt gctgcgttcc
    11641 accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga cggcgatctg
    11701 gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac cgacgaagcg
    11761 ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg ggaagcggag
    11821 ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa agagatggag
    11881 aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga actgtatgac
    11941 cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc caaatccggc
    12001 ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca gatgctgctg
    12061 gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa ttcagcagca
    12121 gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact atgctgccgc
    12181 gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag ttgcccgacg
    12241 gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat aaaccgcgtt
    12301 tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac ggtctggttg
    12361 aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga tgcagcggtg
    12421 aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc agttggtttt
    12481 tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc tattcgctcg
    12541 gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg gttgatgcgg
    12601 cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg gaaaagggct
    12661 tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc gcgcgcaagc
    12721 tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg gtacgtcgca
    12781 tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac gctatcgact
    12841 attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg acgctgatta
    12901 ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg gaaagtctcc
    12961 ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt attggcggta
    13021 cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta acaacgtatc
    13081 tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc tggaaaattt
    13141 aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc gtacacttga
    13201 acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc tggtatggtc
    13261 ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat agcctgcaac
    13321 cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc tgccaaacat
    13381 ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag cctcgcgttt
    13441 cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga cagttttcac
    13501 cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc cgggcggcat
    13561 atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac cttcattgaa
    13621 tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga cgaagccgta
    13681 ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga acccactgat
    13741 gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa cgttgcgcag
    13801 catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat tcaaaaactg
    13861 atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc agtagtgcgt
    13921 tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca tcttaacttc
    13981 cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat cccgtcgctc
    14041 gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt cccctggctg
    14101 ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt accgggcggc
    14161 aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt gatggaagga
    14221 aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct tcacaacggg
    14281 atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg ctttttccag
    14341 catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg caaccgctgc
    14401 gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa acaggcgtgg
    14461 tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat cctgacgccc
    14521 tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca tcaacaccat
    14581 ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct ggtgaccaga
    14641 ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt gcatttttgc
    14701 tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg cgcaacggaa
    14761 ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag ctatgctaaa
    14821 acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg acgtcacatt
    14881 accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt cggcaatcca
    14941 gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac cgcgcatggt
    15001 gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt gccacattca
    15061 taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa cgctgtacta
    15121 catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag aaatgatcct
    15181 ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag agggccagga
    15241 acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt cgtacaaaaa
    15301 atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg cacagatggc
    15361 gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg acgtgacggg
    15421 ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga ctcacccgga
    15481 cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct gttctcgtga
    15541 aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg cacacggtaa
    15601 aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac ctggtagcgc
    15661 gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat caactatgca
    15721 cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct tggcgaatta
    15781 cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg ccttgatacg
    15841 cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc cacctgtagc
    15901 ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct gaccggatta
    15961 acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt gcttcctgca
    16021 tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt ctgggaaccg
    16081 ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt ttggttctgg
    16141 atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga actggcagat
    16201 tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa agcgctgccg
    16261 ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta tcagcaaatg
    16321 catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat tttccaggag
    16381 gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga ggtgcaaaag
    16441 ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac cgtcgccgct
    16501 cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg ttttacgatg
    16561 gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa tccggttggg
    16621 caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca aaaaccgctc
    16681 tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc ggcgctgaaa
    16741 agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag tgtgatgtta
    16801 agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg gatcctgatg
    16861 acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat tgtgaatcgc
    16921 tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt taaaattccc
    16981 gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct gatattgcgc
    17041 aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac cctgcaacta
    17101 ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat tattggttgt
    17161 ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg gttattgcgt
    17221 aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat tcttagcgag
    17281 gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc acataacact
    17341 ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca ttatctggca
    17401 gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa tgccatgacc
    17461 acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta tttacagtct
    17521 tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc ggggagttct
    17581 ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc ggcaggtacg
    17641 ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac catttcgtcg
    17701 atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt gcgggattcg
    17761 aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg acatcttttg
    17821 cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga aacgttgcat
    17881 cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca tcacatccgg
    17941 tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat cacgcgcccg
    18001 acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc gaatatcgct
    18061 aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct ggcgtttcgc
    18121 ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc ccgctactgc
    18181 acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa acgcagaagc
    18241 aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg ctttcgcaga
    18301 ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc cggtccgccc
    18361 catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc acagctcgcg
    18421 caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct ggatcggctc
    18481 aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga gatcgttaaa
    18541 gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt ttggctgccc
    18601 acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg caataatttt
    18661 ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa aggcggtttc
    18721 gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg cctcaatcag
    18781 tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat gccacagaat
    18841 ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca acgccgtaac
    18901 tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga ttcggctgcc
    18961 ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca cttcatcgaa
    19021 agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct gttacgcata
    19081 aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg gcaatcagaa
    19141 atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga atactttctg
    19201 gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata atctcgcggg
    19261 tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc accaccagcg
    19321 aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc tcaccgctat
    19381 gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact ttgccgccaa
    19441 atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag cgcccggcat
    19501 agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg ccaggtgaga
    19561 tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca tcgttgcgct
    19621 taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag gtaaaagaat
    19681 cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc gctattttac
    19741 tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc ggtgagatcc
    19801 cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc ctgccagctc
    19861 agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc cagaccactt
    19921 cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag ccgctcgacg
    19981 aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg catcagggca
    20041 ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg ataaatatca
    20101 acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg atggatagtg
    20161 cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc acgcagcgcc
    20221 gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag ccggttacgc
    20281 atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt atcgctcata
    20341 gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag tgactaaagg
    20401 catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct gaagctccca
    20461 gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg aacgtgtcca
    20521 tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag cgcgggcaag
    20581 attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac taccggtttt
    20641 gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat cttatcggca
    20701 acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg ctgaccatgg
    20761 tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct gtctttgtca
    20821 gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc ggcttcattt
    20881 ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc gccctgaatc
    20941 ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct gttcacataa
    21001 tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag ttcgatgtta
    21061 ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc tgcgggagaa
    21121 agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa catgagattt
    21181 ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc ttcaagaaca
    21241 aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac gatcactttt
    21301 tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc aataaaacat
    21361 ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg ctatgccatt
    21421 ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc acatggatta
    21481 gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg gtgatgggaa
    21541 atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc accttcctca
    21601 acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc gtcccgttga
    21661 aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt ttgatgttca
    21721 gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc agcggcatta
    21781 ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag cgcggttccc
    21841 gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca atgatcgccg
    21901 cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc gggctggtgt
    21961 atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc aaacatgcac
    22021 caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg tttctctctg
    22081 ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg cctgagtatg
    22141 ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac ttctggatgt
    22201 catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat ttgcaacgca
    22261 ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac accggaacac
    22321 cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg atctatacca
    22381 ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg gttaactttg
    22441 tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc ccgattgttg
    22501 aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct gtcgtctttg
    22561 tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc ctgacctctc
    22621 attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc ttcccccgct
    22681 acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa acatgaaatg
    22741 tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca cttaaggagt
    22801 atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt aaggtaggcg
    22861 gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt atcggtaacg
    22921 gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg gccaactttg
    22981 atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac ctctcgtctt
    23041 acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc tacgagaaac
    23101 acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag gagaaggtga
    23161 ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca accggttcct
    23221 acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc tatcgcacca
    23281 ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt gccgttgttg
    23341 gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt attgaaaccc
    23401 acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg ggtggcgagc
    23461 agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa aacacccttg
    23521 agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac ggcagcgaac
    23581 tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag ctggcaaccc
    23641 agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc tgccagactt
    23701 ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt gtatttggtc
    23761 tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc ggtagcgaaa
    23821 acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta gacgtaggcg
    23881 gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac ctcgacgaaa
    23941 gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg ctcggtgcgg
    24001 tactggtggg cgataccagc gactacggta acctgctgca actggtgctg aacgctatcg
    24061 aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc ggcaagccgt
    24121 ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc gacgtcacta
    24181 aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg ctgaaagctg
    24241 aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag gtactgaacg
    24301 cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac tttgcttatt
    24361 cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc gaagaactgc
    24421 tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc ggttcgctgc
    24481 tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg caggattcta
    24541 acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc ccgcgttctc
    24601 cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg cgtgaattta
    24661 atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca cagaaagacg
    24721 atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt catgcctatg
    24781 cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc tacggtgttg
    24841 gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc cgtacgccgc
    24901 acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct cagggtaaag
    24961 acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt aacggcggca
    25021 tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg ctgatcaaat
    25081 atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg cgtaccgcgc
    25141 cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc attgacgaca
    25201 aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa gcggtagtgt
    25261 gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa cacttcatca
    25321 acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag caccgtccgg
    25381 caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga gccagtggaa
    25441 agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc tgttaggtga
    25501 cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga tcagcaacat
    25561 tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac accagggcga
    25621 gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg gcttgtgcat
    25681 ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg gcgtggtgca
    25741 gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca tggtcctgta
    25801 atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt ttgtttttat
    25861 tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc taatgctgcg
    25921 cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc cgccatggcg
    25981 ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt gctcgacccg
    26041 tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct ggtgattatc
    26101 gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt aaaagcgggc
    26161 agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg taacctggtc
    26221 ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct gccggtagat
    26281 accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat ggtactgttc
    26341 ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc gctgcgcact
    26401 gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat cgcgtccggc
    26461 tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg caaccacagc
    26521 gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct gggtaatact
    26581 ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa agcgaatcgt
    26641 ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact aaggggtttt
    26701 tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct gattgtcggc
    26761 ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc tcgcttaacg
    26821 gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg catgttaacc
    26881 ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc gattgcagcg
    26941 acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg tcgcatcttc
    27001 tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat tattgaccgc
    27061 tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc gcgcctgttg
    27121 cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa atatgccggg
    27181 caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg ccgtttctgg
    27241 gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga tcagaaagcc
    27301 attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg tgaagtggtg
    27361 ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact gcaacaaatt
    27421 cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat gaatctggta
    27481 cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg tgtaccgcag
    27541 gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt ggtgcggctg
    27601 aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac actgtgcaac
    27661 gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg ctctgcctat
    27721 tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat taccggacac
    27781 ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca gacgctggtg
    27841 ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga acacggtatg
    27901 cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca gcgcgtgatt
    27961 gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc atcgctaatt
    28021 attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa ccattaattt
    28081 aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc agcaaataaa
    28141 acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta cagcgactta
    28201 caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt tcttaacgag
    28261 cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac atagggaatg
    28321 taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac gttaactgca
    28381 tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct gctgcacccg
    28441 gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta agtctctgac
    28501 agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg agggcgtatt
    28561 tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa ttcaatttga
    28621 taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct tcctccacat
    28681 tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt ttaaatgact
    28741 gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcg
//
LOCUS       DAJTYZ010000044        28677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000044 DAJTYZ010000000
VERSION     DAJTYZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.044"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="OO596_001825"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="OO596_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6717692.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="OO596_001826"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="OO596_001826"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6717693.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="OO596_001827"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="OO596_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6717694.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="OO596_001828"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="OO596_001828"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6717695.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6289..7050
                     /gene="adiY"
                     /locus_tag="OO596_001829"
     CDS             6289..7050
                     /gene="adiY"
                     /locus_tag="OO596_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6717696.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7187..8524
                     /gene="adiC"
                     /locus_tag="OO596_001830"
     CDS             7187..8524
                     /gene="adiC"
                     /locus_tag="OO596_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6717697.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8628..10271
                     /gene="eptA"
                     /locus_tag="OO596_001831"
     CDS             8628..10271
                     /gene="eptA"
                     /locus_tag="OO596_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6717698.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10268..10936
                     /gene="basR"
                     /locus_tag="OO596_001832"
     CDS             10268..10936
                     /gene="basR"
                     /locus_tag="OO596_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6717699.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10937..12037
                     /gene="pmrB"
                     /locus_tag="OO596_001833"
     CDS             10937..12037
                     /gene="pmrB"
                     /locus_tag="OO596_001833"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6717700.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="OO596_001834"
     CDS             complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="OO596_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6717701.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12214..13716)
                     /gene="proP"
                     /locus_tag="OO596_001835"
     CDS             complement(12214..13716)
                     /gene="proP"
                     /locus_tag="OO596_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6717702.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13980..14846)
                     /locus_tag="OO596_001836"
     CDS             complement(13980..14846)
                     /locus_tag="OO596_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6717703.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="OO596_001837"
     CDS             complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="OO596_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6717704.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17484..17819
                     /locus_tag="OO596_001838"
     CDS             17484..17819
                     /locus_tag="OO596_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6717705.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17977..18420
                     /gene="yjdN"
                     /locus_tag="OO596_001839"
                     /pseudo
     CDS             17977..18420
                     /gene="yjdN"
                     /locus_tag="OO596_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18553..19341
                     /gene="phnC"
                     /locus_tag="OO596_001840"
     CDS             18553..19341
                     /gene="phnC"
                     /locus_tag="OO596_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6717706.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19366..20382
                     /gene="phnD"
                     /locus_tag="OO596_001841"
     CDS             19366..20382
                     /gene="phnD"
                     /locus_tag="OO596_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6717707.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20508..21287
                     /gene="phnE"
                     /locus_tag="OO596_001842"
     CDS             20508..21287
                     /gene="phnE"
                     /locus_tag="OO596_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR6717708.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21308..22033
                     /gene="phnF"
                     /locus_tag="OO596_001843"
     CDS             21308..22033
                     /gene="phnF"
                     /locus_tag="OO596_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR6717709.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22034..22486
                     /gene="phnG"
                     /locus_tag="OO596_001844"
     CDS             22034..22486
                     /gene="phnG"
                     /locus_tag="OO596_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR6717710.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22483..23067
                     /gene="phnH"
                     /locus_tag="OO596_001845"
     CDS             22483..23067
                     /gene="phnH"
                     /locus_tag="OO596_001845"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR6717711.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23067..24131
                     /gene="phnI"
                     /locus_tag="OO596_001846"
     CDS             23067..24131
                     /gene="phnI"
                     /locus_tag="OO596_001846"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR6717712.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24124..24969
                     /gene="phnJ"
                     /locus_tag="OO596_001847"
     CDS             24124..24969
                     /gene="phnJ"
                     /locus_tag="OO596_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR6717713.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24966..25724
                     /gene="phnK"
                     /locus_tag="OO596_001848"
     CDS             24966..25724
                     /gene="phnK"
                     /locus_tag="OO596_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR6717714.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25835..26515
                     /gene="phnL"
                     /locus_tag="OO596_001849"
     CDS             25835..26515
                     /gene="phnL"
                     /locus_tag="OO596_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR6717715.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26512..27648
                     /gene="phnM"
                     /locus_tag="OO596_001850"
     CDS             26512..27648
                     /gene="phnM"
                     /locus_tag="OO596_001850"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR6717716.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27648..28205
                     /gene="phnN"
                     /locus_tag="OO596_001851"
     CDS             27648..28205
                     /gene="phnN"
                     /locus_tag="OO596_001851"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR6717717.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28192..28626
                     /gene="phnO"
                     /locus_tag="OO596_001852"
     CDS             28192..28626
                     /gene="phnO"
                     /locus_tag="OO596_001852"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR6717718.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
BASE COUNT         6939 a         7963 c         7403 g         6372 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaacaat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta
     6121 tcctgtaaaa aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact
     6181 cgttattttt atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc
     6241 tttgctttag cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc
     6301 agcgaccaac cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg
     6361 aaagaaccta ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt
     6421 atcgacgtct cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa
     6481 gattacctcc ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct
     6541 acgcccatac tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac
     6601 agcatgatgc ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg
     6661 gtgctatccc gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt
     6721 aattgtgtaa gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat
     6781 cttagtatgg tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagctgaaa
     6841 agcgaaaaca ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat
     6901 gaattaatga tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt
     6961 acgtcgtact ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt
     7021 agtcagcaca gagaacgaac tgtcgcctga tttttaacct taacgaagag ctatattaat
     7081 aacggcatca gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata
     7141 tgacgtaatt tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc
     7201 tgatgctcac aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg
     7261 gtcaggtgtt tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg
     7321 atggttggtg acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt
     7381 cctcgatcca agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct
     7441 cggttatcaa accaacgtcc tctactggct ggcctgctgg atcggcaata tcgctatggt
     7501 ggtcattggc gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt
     7561 aaccatcacc tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa
     7621 aatgatcacc cgtgtgcagg ccgttgccac cgtgctggcg ctgatcccca tcgtcgggat
     7681 tgccgtattt ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag
     7741 cggcctgggc accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat
     7801 cggtgtggaa agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc
     7861 tatcgccacc attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc
     7921 gattatgggg atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc
     7981 cgcacggatg gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg
     8041 ttgcttaggt tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc
     8101 cgatgacgga ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc
     8161 ggggctgatt atcgtcggta ttttgatgac catcttccag ctcagtagca tttcgccaaa
     8221 cgcaaccaaa gagttcggtc tggtatcttc cgtgtcggtc atctttacac tggtgccata
     8281 tctttacacc tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc
     8341 ggcatatctg gctgttacta ccattgcctt cctctactgc atctgggccg tggtggggtc
     8401 cggggcgaaa gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc
     8461 gctgaattac aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga
     8521 ttaattcccc ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa
     8581 ttttgctttg cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc
     8641 tactaaaaag cccctctttg aatttactcg cctggctatt gttggccgct ttttatatct
     8701 ctatctgcct gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc
     8761 tgcataacgt actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg
     8821 tcctgacact aagctctttc ttatggctta atcgaccact ggcctgcctg tttattctgg
     8881 ttggcgcggc tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga
     8941 ttgccaatat tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt
     9001 tattaacgct gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca
     9061 aacctgccac ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg
     9121 tactgctgat tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca
     9181 ataacaaaga gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat
     9241 ggtactccca tcagcgactg gcaaatctac cgctggtgcg aattggtgaa gacgcccacc
     9301 gcaacccgtt aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa
     9361 cctcgcgggc ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg
     9421 cgaaagataa cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt
     9481 cagtaccgtg catgttctcg gatatgccgc gtgagcatta caaagaagag ctggcacagc
     9541 accaggaagg cgtgctggat atcattcagc aagcgggcat caacgtgctg tggaatgaca
     9601 acgatggcgg ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga
     9661 atctgcctgg tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg
     9721 aagagtacat caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc
     9781 acggtccgac ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg
     9841 acaccaatga gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc
     9901 tggtttacgt cgactatatt gttgataaag cgattaatct gctgaaagaa catcaggata
     9961 aatttaccac cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca
    10021 tctatctgca cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccgatgc
    10081 tgctgtggct gtcggaggat tatcaaaaac ggtatcaggt tgaccagaac tgcctgcaaa
    10141 aacaggcgca aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa
    10201 ctggcgttga gacgaagtat taccaggctg cggatgatgt tctgcaaact tgcaggagag
    10261 tgagtgaatg aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct
    10321 ggcggcgcaa accgaaggct acacgtgcga tggcgtaaca accgcgcgga tggcggaaca
    10381 aagccttgag gccggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga
    10441 tggactgcat tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct
    10501 caccgctcgc gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta
    10561 tctggtgaag ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg
    10621 ccataataat cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg
    10681 ccgtcaggta tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct
    10741 gtcacggtta atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat
    10801 ctataactgg gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg
    10861 cgacaaagtg ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc
    10921 gaatgaggaa aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca
    10981 acggctgata ttgaccatcg gggccatttt gttggtgttt gagctgatca gcatcttctg
    11041 gctatggcat gaaagtaccg agcagattca gctgtttgag caggcgctac gcgacaatcg
    11101 caacaacgat cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc
    11161 cggcgtcttt atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac
    11221 ccgcccgctg gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc
    11281 cattgccatt cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct
    11341 ggtcagtcgc ctgacgagca cgctggataa cgaaaggtta tttaccgctg acgtcgcgca
    11401 cgaactgcga acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca
    11461 tcacattgat gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca
    11521 gctgctgcaa ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa
    11581 actgctggaa gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg
    11641 acagcaaacc ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac
    11701 cctgctgcgg atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccacaagg
    11761 cagcaacatt atgattaagc tgcaagaaga tggcggagcg gtcatggccg ttgaagatga
    11821 aggaccgggt attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga
    11881 cagccgttat ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca
    11941 tcacgggcag tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg
    12001 gctgaagaaa gatcagtacg tagcaaacca gatataaaga aagctgctga ccaccagcac
    12061 actgaacacg gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa
    12121 cctggaggag agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat
    12181 aagacagcgt cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg
    12241 caccaggcgg gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat catcgatttt
    12301 ctgctcgata ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc
    12361 cgccggtgtc gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat
    12421 taaaccaatc accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct
    12481 ttcgaccagc caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa
    12541 tgccgccgcc agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc
    12601 cataacgccc gtaaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag
    12661 gccgatgacg ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagggcaac
    12721 actaccaagt agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg
    12781 ctggacaaac agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga
    12841 gtaatgcagg ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac
    12901 gttggtggca attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat
    12961 ctctttaaac gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt
    13021 atcaacatgc tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat
    13081 aatccctaac ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc
    13141 ttcgccgaca atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat
    13201 agaaccgaag tccagccagc tgcccataaa gccacgttta cggtcagggg agtattccgc
    13261 aacaaatatc gacgccccgg tatattcacc gccgaccgag aaaccttgtg ccatcttaca
    13321 gatcaacagc agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat
    13381 acagaacgta ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata
    13441 tttatcgccc aacataccaa agaagagccc gccaagcggt cgaatcagaa agggaacgga
    13501 gaaagtggca agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt
    13561 acctaatgcg taagcaacaa aaccataaac accaaaatcg aaccattcca ttgcattacc
    13621 cagtgatgct gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg
    13681 aagggtaatc ggttttactt ttttcctttt cagcatagct ttcctcgcag agttggccca
    13741 tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg
    13801 atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca
    13861 atcaaaccag ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta
    13921 aatttgtgat caacttcaca catggatttc gtttaggact cattgatgta actgattatt
    13981 tacgccaaat ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa
    14041 cagagtcgtg gcatccccag cgcgactgtt tgccagcgtt gggtcactga ccagcaaacg
    14101 ggctgtttat ctgccgtgga ccactttccc agccaactta aaaaatcagc atgctcccct
    14161 cccgaagagg caaaccactg atggtaatag tgacgcgtgg tcggtgccgc attgatactc
    14221 tctgccagat gttgtgtcgc tttctcgcgc aaatcaccca gtaacaacac caccgcttgc
    14281 gggcttccca gacggcaggc atcgccaaat gcaccccagc gcaactcctc cagaacgata
    14341 aaacagcgcc caggcaacga ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt
    14401 tgctcgctct gatcacctgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc
    14461 cagcgacaac gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctccggg
    14521 aaagcagagt gctggcggtg atccattttt tgcagcagcg ccagatgctg cgcgatctgc
    14581 gtcagcgcca gatgaccggg atccagcatc gacgccagtt tttccaccat tgtcagtcgc
    14641 aggctaagcg gcgcatttaa catgccgccg ttcgtccatg tctgaagctg cacacgcgaa
    14701 agaagttcct gcatcagtcg ttcacgaaac tgctgctgat gggcttgtgg aaggcgggca
    14761 tcatcgccct gcgccagatc cactaaaaag cggggataaa ccgactccag cacgcgaccg
    14821 gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat
    14881 cgcgtaacag ctgggcgtcg gtgtaaatcc agccgtgagt catcacagtc tgctgcaatt
    14941 gctgctgcat cagcctgacc accgattcat tttgttgacg cagagccagg ctttcgcgta
    15001 aacgcgtctg taattccgtc aaacatgaag cgaactcagc gcaaaaagta ttcatgcctg
    15061 ccgtaacaga ttcatcgacc tgctctgcca gaactttagc catttgttgg caataaagat
    15121 cgacttctgc gcttaaagct cgttgcaaca cactgtaatc aaccgtctct gtcggggatt
    15181 tctcatttcc ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa
    15241 cgcctaaacg cgtgctctgc tcgtccgttg catcctggcg cgaaatgact tcactgaaca
    15301 gctggcgagt gttgacgtgg ggaactactc cgtgaaaaac aggaaaatga accccaggac
    15361 gaaaccctga ctcgctcaat tccattttga cttgttgctc aatgggccga ataacatcgg
    15421 ttaacacccg gcaaagggtg gattcaagct cggcaaaacg cagcgtaaag tcgcgactga
    15481 tggtgttctg cgccgtctgt aacagtgtct cacagcaggt acgaatctcg cttaacggct
    15541 ccgaatcatc ctgaaacaag gcggctaact gcgcattcag cgcatcttgc tgttgacgca
    15601 gaaagtggtt ggcggaggtc agggccagct cgatttcatg tttaatctcg ccgctcacct
    15661 gcgcctgatt gagttgcaat agctgcaaac tttcttcgac ctgatggata ttttgccgca
    15721 attgttcaca agcgacgttt aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct
    15781 gcgcgtaatt caacagttta tgcgcagcag atcgcagagc atacaacgag gcgttagcgt
    15841 aagcggcatg aagcaacgcc tgaattggct gggcgaacag cgaatcttcc cacaactgat
    15901 cggcagcatg acgaatatgt tcgaggtccg ccagatcggc atgacgccag cgcctgccga
    15961 gcgcggcatg ggcaaaatct tccacccagc gttgttgctc tggcgctggt aacttaccgt
    16021 tgttggctaa ctcatggcgc gctcgatttg ccaagtagcc ccacatcgac gacaccggaa
    16081 atatctgctg tggcgtaata cagcctttca tcagcgtccc ggaaatcagt gcccgcacct
    16141 ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt attgaccagc acatacagtg
    16201 gcaccgattg ccccaccgcc aaaatcgcct cacggacctc ttcatcggag atcgatttca
    16261 gttgcgtata atccagcacc gccagtaccg ccgaggcgcg tgccagctgc tggttaagca
    16321 ttttttgcag atgcggttgc ccggcttcat ttggcccggg ggtatccagt aacgtcaact
    16381 gaccgggata actctccagc cccgccagat ggacaaactc cacttcaatc acgggaatat
    16441 gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca
    16501 ctaaatcatt caaacttttc agacaatgaa aaataggctg ggcacccaga taatattttt
    16561 cgaaagcgac gccattttcg atccgctgca taagcgcacg catatcttta tctatttcca
    16621 gcacatcggt cagatgctta atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta
    16681 aacaatcgat tggcgcgaca tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat
    16741 ggcgaataag cgtcggcagc gcagtcattg ggcgattacg attaggcaga acctccgtac
    16801 caacaatggc attaatggtg gttgatttcc ctgctttcat agtaccgaca atcgccagca
    16861 ccatttccag tcgggaaatt ttactcaact cattattcag catcgcgtga cgttcggcga
    16921 tattaggctg actccagggt aaagccagtt gtggcgcgtc gtctccgggt acagagagag
    16981 gcattttttc cagtaactgc aactgttggc gagaaagctg taacaggcgt tcagcctcct
    17041 gacttaactc atacagggtc tgtgtgtaca tagaaaattc ttccttaaag caaattttgt
    17101 tacttttatt tagccagatt gtttttgagt tctgtttgag ttctgttttt ggcttttata
    17161 attactgcaa gaaataattt tatatttagt gtgttgtttt ttatcagaat aaataacgtc
    17221 ttctgatgcg tttaaaacgt cagaaagata aaaatatcat gtgaataaaa aaaagaacaa
    17281 gtagagcatt aacattatct taaataataa atagaggcaa aaagattatt ttctttttgc
    17341 gtttcctttc aaatgaaaac gatcgtcgtc taaaatcagc agtacccccg acaaactcag
    17401 ggattttgtg tataattgcg gcctttttcg gcaatctgcc gttttttggc gcttttgccc
    17461 tgctgacttt tgaggaaatc cacatgtcat taccacactg cccaaaatgc aactccgaat
    17521 acacttacga agataacggc atgtacatct gcccggaatg tgcctacgaa tggaacgacg
    17581 cagaacctgc acaggaaagc gacgagctga tcgttaaaga tgctaacggc aatctgctgg
    17641 ctgacggcga cagcgttacc atcattaaag atctgaaggt gaaaggtagc tcttcgatgc
    17701 tgaaaattgg caccaaagtg aaaaacatcc gcctggttga aggcgaccat aacatcgatt
    17761 gcaaaatcga cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat
    17821 tgtattgtga tcggtaagcc ggataaggcg ctcgcgccgc atccggcaac gatgccgact
    17881 gcctgatgcg acgcttgcgc gtcttatcag gcagcctctc atctgtataa atttcgaact
    17941 acacttaatt ggcttctctt aactgaggtc accatcatgc cgttaagtcc ctacctctct
    18001 tttgccggta actgtgccga cgcaattgct tattatcaac gtacgttggg cgctgaactg
    18061 ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc cgagaactgc
    18121 ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaatgtgcg cattgccgga
    18181 agcgacatca tgatgagcga tgccatgccg tcaggaaaag ccagctactc cggctttacg
    18241 ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa tcttgccgct
    18301 aacggaaaaa tcgaaatggc ctggcaggaa actttctagg cgcatggctt tggcaaagtc
    18361 accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa
    18421 cccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt
    18481 agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag
    18541 ggaggctgca tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag
    18601 catcaggcgc tgcatgcggt tgctctgaac attcatcacg gtgaaatggt ggctctgctt
    18661 gggccgtcgg gttccggcaa atccaccctt ttacgtcact taagcggttt gattaccggc
    18721 gataaatccg ccggcagcca tatcgagctg ctgggccgca cagtccagcg cgaaggccgt
    18781 ctggcgcgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca acaattcaac
    18841 ctggtgaacc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg
    18901 ttctggcgca cctgttttag ctggtttacc cgcgagcaga aacaacgcgc gttacaggcg
    18961 ctgacccgcg ttggcatggt gcattttgcc catcaacgcg tttccaccct ctccggcgga
    19021 cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt gattctggcc
    19081 gatgaaccca tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga caccctgcgc
    19141 gacatcaatc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc
    19201 ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgttttcta cgacggcagc
    19261 agccaacagt ttgataacga acgttttgac catctctacc gcagcattaa tcgcatcgaa
    19321 gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa cgctaagata
    19381 attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag cccggcacac
    19441 gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac
    19501 ctgaaaccgc aatggacgcc attcttacag gatatggaga agaagctggg cgtgaaggtg
    19561 aacgccttct ttgccccaga ctacgcaggc attatccagg gaatgcgctt caataaagtg
    19621 gatatcgcct ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc caacggccag
    19681 gtcttcgccc agacggtcgc ggcggatgga tcgccaggtt actggagcgt gttgatcgtc
    19741 aacaaagata gtccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc
    19801 ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttt
    19861 gccaaaaaca atatctccgc cagcgacttt aagcgcaccg tcaacgccgg gcatgaaacc
    19921 aacgcgctgg ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc
    19981 gacaagctga aaacctccgc gccagagaag ctgaaagaac tgaaggtgat ctggaagtcg
    20041 ccgctgatcc caggcgatcc gatcgtctgg cgcaagaatc tctccgaaac caccaaagac
    20101 aagatctacg acttctttat gaactacgga aaaacgccgg aagagaaagc ggtgctggaa
    20161 cgcctgggct gggcaccgtt tcgcgcctcc agcgacctgc aactggtgcc gattcgccag
    20221 ctcgcactgt ttaaagagat tcagggcgta aaaagcaata acggactgaa tgagcaggac
    20281 aagctggcga aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac
    20341 gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt aatgtgctcg atcggtcccc
    20401 tctcccttga gggagagggt tagggtgagg gggaaatgcg cacgctgatg ccctcacccc
    20461 ggccctctcc cacagggaga gggagaaaac attaaacgga gccaaacatg caaaccatca
    20521 ccatcgcccc acccaagcgc agctggttct cgcttctgag ctgggccatt gttctcgccg
    20581 tgttggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatc aaagacggcg
    20641 gcaacatggc gaccttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt
    20701 acctcaccga aatggccgtc acgctgcaaa tcgccgtctg gggcaccgcg ctggcagtgg
    20761 ttctctccat ccccttcggc ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc
    20821 agcccgttcg ccgcctgatg gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc
    20881 tgttcgtggt cgccgtcggt ctcggaccgt tcgctggcgt gctggcgcta tttattcaca
    20941 ccaccggcgt gctctcgaaa ctgctttccg aagcggtgga agcgattgaa cccggcccgg
    21001 tggaaggcat tcgcgccacc ggcgccaaca agctggaaga gatcctttac ggcgtgctgc
    21061 cacaggtaat gccgctgctg atctcctact ccctctatcg cttcgaatcc aacgtccgct
    21121 cggcgaccgt cgtcggcatg gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga
    21181 ttcgcggttt ccagttccaa caaacctgcg ccctgatggt gcttatcatc gtcacggtca
    21241 gcctgctgga tttcctctct caacggttgc gtaagcactt tatctgataa gtgaggcatt
    21301 gatatctatg cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc
    21361 cgcaaaactt gagcaggagc ttcgtcaaca ctatcgctgc ggcgactatc ttcccgccga
    21421 gcagcaactg gcggcgcgct ttgaggtaaa ccgccacacc ctgcgccgcg ccatcgacca
    21481 actggtggaa aaaggctggg tacagcgccg tcagggcatc ggcgtgctgg tgctgatgcg
    21541 cccgttcgat tacccgctca acgcccaggc gcgttttagc cagaacctgc tggaccaggg
    21601 cagccatccc accagcgaaa aactgctgtc ggtattgcgc cccgcgtccg gtcacgttgc
    21661 tgacgcgctg gggattaccg agggtgagaa cgttatccat ttgcgcaccc tgcgtcgcgt
    21721 caacggcgtc gcgctctgtt taatcgacca ctactttgcg gacctcaccc tctggccgac
    21781 actgcaacgt ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa ccggaattgc
    21841 gctgcgccgc agccagacgc ggatcagcgc ccgccgcgcc caggccaaag agtgccagcg
    21901 ccttgaaatc ccgaatatgt cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg
    21961 tgaaagcagc ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac
    22021 tatggagcac tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtactggc
    22081 gcacagccaa ccggctgaac tggcagcccg cctgaacgcg ctgaacatca ccgccgacta
    22141 tgaagtgatc cgcactgccg aaactggcct ggtgcagatt caggcgcgaa tgggcggtac
    22201 cggcgaacgc ttttttgccg gcgacgccac gctgacccgt gccgccgtac gcctgactga
    22261 cggcaccctc ggctacagct gggtgctggg gcgtgataaa cagcacgccg aacgctgcgc
    22321 gctgattgat gcgctgatgc agcaatctcg ccactttcaa aacttatcag aaacccttat
    22381 tgccccgctg gacgctgacc gtatggcacg cattgccgca cgccaggccg aagtgaacgc
    22441 cagccgggtc gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct
    22501 tttatgcttc ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc
    22561 gagccgggcg tgattgtcgc cctgtatcag ctcaaacgcg gctggcagcc gctgaatatc
    22621 gccaccacca gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gcttgccacg
    22681 ccattaagta acgatatcgt caatcagagc ctgcgttttc ataccaacgc gccgctggtc
    22741 agccagccgg aacaggcgac cttcgcggtg acggatgagg cgatttccag cgaacagctc
    22801 aacgcccttt ccaccgacac cgccgttgcg ccggaagcgg gcgcgacgct gattttacag
    22861 gtcgccagcc tgagcggcgg acgcatgttg cgtctcaccg gcgcgggcat tgccgaagaa
    22921 cgaatgatcg ctccgcagct gccagagtgc attctgcacg aactcaccga gcgcccgcac
    22981 ccgttcccgc tcggcatcga cctaatcctg acctgcggcg agcgcctgct ggctatcccg
    23041 cgaaccactc atgtggaggt gtgctgatgt acgttgccgt gaaagggggc gaaaaggcga
    23101 tcgacgccgc ccacgccctg caagagagcc gacgccgggg cgataccgat ttgcccgaac
    23161 tgagcgtcgc ccagattgaa cagcagctta acctcgcggt ggatcgcgtg atgacagaag
    23221 gcggcattgc cgaccgcgaa ctggcggcgc tggcgctgaa acaggccagc ggcgataacg
    23281 tcgaagcgat tttcctgcta cgcgcctacc gcaccacgct gacgaaactg gcggtaagcg
    23341 agccgctcga caccacggag atgcgtctcg aacggcgtat ttcagcggtt tataaagata
    23401 ttcccggcgg ccagctgctt ggcccaacct acgactacac ccatcgcctg ctcgatttca
    23461 ctctgctggc aaacggcgaa gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt
    23521 cgccgcacgt tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaggatagcg
    23581 gagcacagcc ggatgacatc acccgcacgc cgccggttta cccctgttca cgttcttccc
    23641 gtttgcagca gttaatgcgc ggcgacgaag gctatttgct ggcgctggct tactccaccc
    23701 agcgcggtta cgggcgcaac cacccgttcg cgggcgagat ccgcagtggt tacatcgacg
    23761 tgtcgattgt gccggaagag ctgggatttg cggtaaacgt cggcgaactg ctgatgaccg
    23821 agtgtgaaat ggtcaacggt tttatcgacc cgccggatga gccgccgcac ttcacgcgcg
    23881 gctacgggct ggtattcggc atgagcgagc gcaaagcgat ggcaatggcg ctggttgacc
    23941 gtgcgttgca agccccggaa tacggcgagc acgcgacagg cccggcgcag gatgaagagt
    24001 tcgtgctggc gcatgccgac aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc
    24061 cgcactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga
    24121 accatggcta atctgagcgg ctacaacttt gcctacctcg acgagcagac caaacgcatg
    24181 atccgccgcg ccatcttaaa agcggtggcg atccccggtt atcaggtacc gtttggcggg
    24241 cgcgagatgc cgatgccgta cggctgggga actggtggta tccagctcac cgccagcgtg
    24301 attggcgaaa gcgacgtgtt gaaggtgatt gaccagggtg cggatgacac caccaacgcc
    24361 gtgtcgattc gcaacttctt taagcgcgtg accggggtaa acaccactga atgtacggac
    24421 gatgcgacgc ttatccagac gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag
    24481 atcattatct tccaggtgcc aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg
    24541 gagacccgca ctatgcacgc gctggaggag tacggcgtga tgcaggtgaa actgtatgaa
    24601 gatatcgccc gcttcggtca tatcgccacc acctatgctt atccggtgaa ggtgaacggg
    24661 cgctacgtga tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg
    24721 cccgccctgc agctgttcgg cgcagggcgc gagaagcgca tctatgcggt gccgccgttt
    24781 acccgcgtgg aaagtctcga tttcgacgat cacccgttca ccgttcagca gtgggatgag
    24841 ccatgcgcca tctgcggatc gacccacagc tatcttgatg aagtggtgct ggatgacgcc
    24901 ggaaaccgca tgtttgtctg ctccgatacc gattattgcc gccaacagag cgaggcgaaa
    24961 agccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttac gcgccgggca
    25021 aaggctttag cgatgtctct tttgatttat ggccggggga agtgctgggc attgtcgggg
    25081 aatccggctc cgggaagacc acgctgctga agtcgatctc cgcgcgtctg acgccgcagc
    25141 agggggagat tcactatgaa aaccgttccc tctatgcaat gagcgaggcc gaccgccgtc
    25201 gcctgctgcg caccgaatgg ggcgtggtgc atcagcatcc gctcgacggc ctgcgccgcc
    25261 aggtgtcggc aggcggcaat atcggcgagc ggctgatggc gaccggggcg cgacattacg
    25321 gcgatattcg cgctaccgcg cagaagtggc tggaagaggt ggagattccc gccaaccgta
    25381 tcgacgacct gccgaccacc ttttccggcg gtatgcagca gcgtttgcag attgcccgca
    25441 acctggtgac gcatccgaag ctggtgttta tggatgaacc gaccggcggg ctggatgtgt
    25501 cggtgcaggc ccgcctgctc gacctgctgc gcggactggt ggtggagctg aacctcgcgg
    25561 tagtgattgt cacccacgat ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga
    25621 tgaagcaggg gcaagtggtg gagagtgggt taaccgaccg cgtgctcgac gacccgcatc
    25681 atccgtatac acagctgctg gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg
    25741 gcgtaaacgc cttatccggc ctacaaatgc gctccccgta ggtcggataa gacgcgtcag
    25801 cgtcgcatcc gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa
    25861 aaccttcatc ctgcaccagc aaaacggcgt gcgcctgccc gtcctcaacc gcgcctcgct
    25921 catcgtcaac gcgggcgaat gcgtggtgct ccacggccat tccggcagcg gaaaatcaac
    25981 cctgctacgc tcgctgtacg ccaactatct gcccgacgaa gggcaaatcc agatcaaaca
    26041 cggtgacgaa tgggtcgatc tggtcaccgc gccagcgcgc aaagtggtgg agatccgcaa
    26101 aaccaccgtt ggctgggtga gccagtttct gcgcgtcatc ccgcgtattt cggcgctgga
    26161 agtggtgatg cagccgctac tcgataccgg cgtaccgcgt gaagcctgcg ccgctaaagc
    26221 cgcgcgtctg ctcacccgcc tgaacgtgcc ggaacgcctg tggcatctgg cgccatcgac
    26281 cttctccggc ggcgaacagc agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc
    26341 cattctgctg ctcgacgaac ctaccgcctc gctggacgcc aaaaacagcg ccgccgtagt
    26401 cgagctgatt cacgaagcca aagcccgtgg cgcggccatc gtcggcatct tccacgacga
    26461 agccgtacgt aatgacgttg ccgaccgcct gcacccaatg gaagcctctt catgattatc
    26521 aataacgtca aattggtgct ggaaaacgaa gtggtacacg gttcactgga ggtgcaggac
    26581 ggcgaaatcc gcgcctttgc cgaaagccag agccgcctgc cggaggcgat ggacggcgaa
    26641 ggcggctggc tgctgccggg gctgattgag ctgcataccg ataatctgga taaattcttc
    26701 accccgcgcc cgaaagttga ctggcctgcc cactcggcga tgagcagcca cgacgcgctg
    26761 atggtggcga gcggcatcac caccgtactg gatgccgtgg caattggcga cgtgcgcgac
    26821 ggcggcgatc ggctggtgaa tctggagaag atgatcaacg ccatcgaaga gacgcagaaa
    26881 cgcggcgtca accgcgccga gcaccgtctg catctgcgct gcgaactgcc gcatcacacc
    26941 acgctgccgc tgtttgaaaa actggtgcag cgcgagccgg tgacgctggt gtcgctgatg
    27001 gaccactcgc cgggccagcg ccagttcgcc aatcgcgaga agtatcgcga atattatcag
    27061 ggcaaatact ccctcactga tgcgcagatg cagcagtacg aagaagagca actggcgctc
    27121 gccgcacgct ggtcgcagcc gaatcgcgaa tccatcgccg ccctgtgccg cgcgcgaaaa
    27181 attgcgcttg ccagccacga tgacgccacc cacgcccacg ttgctgaatc tcaccagctt
    27241 ggcagcgtga tcgccgaatt tcccaccacg ttcgaagcgg cggaagcctc gcgcaagcat
    27301 ggcatgaacg tgctgatggg cgcgccgaat attgtgcgcg gcggctcgca ctccggcaac
    27361 gtggcggcca gtgaactggc gcagcttggc ctgctggata tcctctcttc cgactactac
    27421 cccgccagcc tgctcgatgc ggcatttcgc gtcgccgatg acgagagcaa ccgctttacg
    27481 ctgccgcagg cggtgaagct ggtgactaaa aatccagcgc aggcgcttaa tctccaggat
    27541 cgcggggtga ttggcgaggg caaacgcgcg gacttggtgc tggcgcatcg caagggcaat
    27601 cacattcata tcgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac
    27661 tgatttggtt aatggggccg tccggctccg ggaaagatag cctgctggcg gaactccgcc
    27721 tgcgggaaca aactcagcta ctggtggcgc atcgctacat cacgcgcgac gccagcgccg
    27781 gaagcgaaaa ccatatcgct ctgagcgagc aggagttttt tacccgcgcg gggcagaacc
    27841 tactggcctt aagctggcac gctaacggtc tgtattatgg cgtcggcgtc gagatcgacc
    27901 tctggctgca cgccggattc gacgtgctgg tcaacggctc acgcgcccat ctgccgcagg
    27961 cgcgggcgcg ctatcaatcg gcgctgctgc ccgtctgttt acaggtttcg ccggagatcc
    28021 tccgccagcg cctggaaaac cgtggccgtg aaaacgccag tgaaattaac gcccgcctgg
    28081 cgcgcgccgc ccgctatact ccacaggatt gccatacgct caacaatgac ggcagcctgc
    28141 gccagtcggt cgacacgctg ctgacgctga ttcatcagaa ggagaaacac catgcctgct
    28201 tgtgagcttc gcccggccac gcagtatgat accgacgcgg tttacgcgct gatttgtgag
    28261 ctaaaacagg cggagtttga ccaccacgca tttcgcgtgg gctttagcgc caatctgcgc
    28321 gacccgaaca tgcgctatca tctggcgctg ctggatggcg aggttgtcgg catgatcggc
    28381 ctgcatttgc agtttcatct gcatcatgtc aactggatcg gcgaaattca ggaactggta
    28441 gtgatgccgc aggcgcgcgg cctgaacgtc ggtagcaagt tgctggcgtg ggcagaagaa
    28501 gaagcccgcc aggccggggc cgaaatgacc gagctttcga ccaacgtgaa gcgccacgac
    28561 gcgcaccgtt tctatctgcg agaaggctac gagcagagtc acttccgctt caccaaggcg
    28621 ctgtaacatg agtctgaccc tcacgctcac cggcaccggc ggcgcacagg gcgttcc
//
LOCUS       DAJTYZ010000045        28086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000045 DAJTYZ010000000
VERSION     DAJTYZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.045"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            44..1264
                     /locus_tag="OO596_001853"
                     /pseudo
     CDS             44..1264
                     /locus_tag="OO596_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1237..1866
                     /locus_tag="OO596_001854"
     CDS             1237..1866
                     /locus_tag="OO596_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6717719.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1867..3027)
                     /gene="ybdL"
                     /locus_tag="OO596_001855"
     CDS             complement(1867..3027)
                     /gene="ybdL"
                     /locus_tag="OO596_001855"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6717720.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3136..4224
                     /gene="hcxA"
                     /locus_tag="OO596_001856"
     CDS             3136..4224
                     /gene="hcxA"
                     /locus_tag="OO596_001856"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6717721.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4234..4431)
                     /locus_tag="OO596_001857"
     CDS             complement(4234..4431)
                     /locus_tag="OO596_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6717722.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4613..6718)
                     /gene="cstA"
                     /locus_tag="OO596_001858"
     CDS             complement(4613..6718)
                     /gene="cstA"
                     /locus_tag="OO596_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6717723.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6899..7312)
                     /gene="entH"
                     /locus_tag="OO596_001859"
     CDS             complement(6899..7312)
                     /gene="entH"
                     /locus_tag="OO596_001859"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6717724.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7315..8061)
                     /gene="entA"
                     /locus_tag="OO596_001860"
     CDS             complement(7315..8061)
                     /gene="entA"
                     /locus_tag="OO596_001860"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6717725.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8061..8918)
                     /gene="entB"
                     /locus_tag="OO596_001861"
     CDS             complement(8061..8918)
                     /gene="entB"
                     /locus_tag="OO596_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6717726.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8932..10542)
                     /gene="entE"
                     /locus_tag="OO596_001862"
     CDS             complement(8932..10542)
                     /gene="entE"
                     /locus_tag="OO596_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6717727.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10552..11727)
                     /gene="entC"
                     /locus_tag="OO596_001863"
     CDS             complement(10552..11727)
                     /gene="entC"
                     /locus_tag="OO596_001863"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR6717728.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12102..13067
                     /gene="fepB"
                     /locus_tag="OO596_001864"
     CDS             12102..13067
                     /gene="fepB"
                     /locus_tag="OO596_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6717729.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(13148..14398)
                     /gene="entS"
                     /locus_tag="OO596_001865"
     CDS             complement(13148..14398)
                     /gene="entS"
                     /locus_tag="OO596_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6717730.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14509..15513
                     /gene="fepD"
                     /locus_tag="OO596_001866"
     CDS             14509..15513
                     /gene="fepD"
                     /locus_tag="OO596_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6717731.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15510..16502
                     /gene="fepG"
                     /locus_tag="OO596_001867"
     CDS             15510..16502
                     /gene="fepG"
                     /locus_tag="OO596_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6717732.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16499..17314
                     /gene="fepC"
                     /locus_tag="OO596_001868"
     CDS             16499..17314
                     /gene="fepC"
                     /locus_tag="OO596_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6717733.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17311..18444)
                     /gene="fepE"
                     /locus_tag="OO596_001869"
     CDS             complement(17311..18444)
                     /gene="fepE"
                     /locus_tag="OO596_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6717734.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18660..22541)
                     /gene="entF"
                     /locus_tag="OO596_001870"
     CDS             complement(18660..22541)
                     /gene="entF"
                     /locus_tag="OO596_001870"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6717735.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22538..22756)
                     /gene="ybdZ"
                     /locus_tag="OO596_001871"
     CDS             complement(22538..22756)
                     /gene="ybdZ"
                     /locus_tag="OO596_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6717736.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22759..23961)
                     /gene="fes"
                     /locus_tag="OO596_001872"
     CDS             complement(22759..23961)
                     /gene="fes"
                     /locus_tag="OO596_001872"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6717737.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24204..26444
                     /gene="fepA"
                     /locus_tag="OO596_001873"
     CDS             24204..26444
                     /gene="fepA"
                     /locus_tag="OO596_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6717738.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26619..27239
                     /gene="entD"
                     /locus_tag="OO596_001874"
     CDS             26619..27239
                     /gene="entD"
                     /locus_tag="OO596_001874"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6717739.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27361..27513)
                     /gene="hokE"
                     /locus_tag="OO596_001875"
     CDS             complement(27361..27513)
                     /gene="hokE"
                     /locus_tag="OO596_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6717740.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27864..28016)
                     /gene="hokE"
                     /locus_tag="OO596_001876"
     CDS             complement(27864..28016)
                     /gene="hokE"
                     /locus_tag="OO596_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6717741.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
BASE COUNT         6734 a         7862 c         7278 g         6212 t
ORIGIN      
        1 ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta tttataaaat
       61 tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga cactcaacac
      121 cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa tgctgcatct
      181 gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta tcgactggga
      241 ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt acaccgatgt
      301 catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc tttcacaata
      361 ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc ccccgcaaga
      421 tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct tcgaacaatt
      481 tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga tgatcggcat
      541 ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata aacaacgttt
      601 tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt acatttaccc
      661 catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc aaaatctctg
      721 caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata tgcgaatttg
      781 agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta tcgaaccgga
      841 tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca tttacgccgg
      901 acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt tagactggca
      961 agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc attaccgcaa
     1021 taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc cacaaaccca
     1081 gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca aagttttact
     1141 caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga agaactatca
     1201 gcgttataac gaacggataa aaggaaaacg tcaggaatgg gggatactat gcaacaacga
     1261 ttaacgcagg atttaacgca atttctcgcc agcttgccgg aagatgatcg catcaaagcg
     1321 attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga gccagtggat
     1381 tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc aaacaacgtg
     1441 gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt tacccaaccc
     1501 attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt tcaccgccat
     1561 gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt gccggtgacc
     1621 tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa tcgcgctcgt
     1681 ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca gttgggatgg
     1741 gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct gcgcctgaag
     1801 caaattaacg gcctgcaaga gctgtttgcc gaccgtcaat attcccgcgc ctggacggtt
     1861 aaataactaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt ccttcttggc
     1921 aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg acagcggaat
     1981 cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat ccagggtaga
     2041 aaccgcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa tctccagccg
     2101 gctttcattt aaggcattta ccagaatatc gcgcttctgg cgataaaagt ccggtaacgc
     2161 aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg ccggggtatt
     2221 caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg gcgctggcgc
     2281 aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg aagaaaccgc
     2341 cactgcccgc tcacgcagct gcgaatgcgc cagcacactg gcatggccct gttgtgaaaa
     2401 gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg cgatcgcctg
     2461 ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggagttat gcggagtgtt
     2521 gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc agtcaacgcg
     2581 aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg cgatggcggg
     2641 ggcgtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc gcaccagtgc
     2701 ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat cgctatcggc
     2761 atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc gcaaggcctg
     2821 cacgccggtc atgggcgcgt attggtttgc cccctgggca acgtggtacg ccagccgctc
     2881 ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa tcgcctggtg
     2941 ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg gaagtttgct
     3001 ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg cgtgttgaag
     3061 gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta aataaaacaa
     3121 taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc ggctaactac
     3181 ttttcacatc caggaagttt caatcacctg aacgattttt tcactgatga acaactttct
     3241 cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa acttccgcca
     3301 gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag cgaaagcgat
     3361 gtacagcaac tggcggctga gtccggtgac gatcgcagcg tggtgattgg cgtcggcggc
     3421 ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc atttgttgcc
     3481 gttccgacga tcgccgctac ctgcgccgcc tggacaccgc tctccgtctg gtataacgat
     3541 gccggacagg cgctgcatta tgagattttc gacgacgcca attttatggt gctggtggaa
     3601 ccagagatta tcctcaacgc gccgcaacaa tatctgctgg cggggatcgg tgacacgctg
     3661 gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc gctaaccgtg
     3721 cgactgggga tcaataatgc gcaggccatt cgcgacgtat tgttaaacag tagcgaacag
     3781 gcgctgagcg atcagcaaaa tcaacagtta acgcaatcat tttgcgatgt ggtggatgct
     3841 attattgctg gtggtgggat ggtcggtgat ctgggcgatc gttttacgcg tgtggcggcg
     3901 gctcatgccg tgcataacgg tctgactgtc ctgccgcaaa ccgagaaatt tctccacggc
     3961 accaaagtcg cttatggcat tctggtacaa agcgccttgc taggtcagga tgatgtgctg
     4021 gcgcaattaa ccggggcgta tcagcgtttt catctgccga ccacgctggc ggagctggaa
     4081 atggatatca ataatcatgc ggagatcgac aaagtgatta cccacaccct gcgtccggtg
     4141 gaatacattc attacctgcc agtcacgctg acaccagata cgttgcgtgc agcgttcgaa
     4201 aaagtggaat cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc cgccttttcc
     4261 accgtagcgc gcgtcctgcc gctcacggaa aaactcttcg taggtcatcg gcgtttgatc
     4321 gggatggtta acccgcatat gttcgacata gttgtcgtaa tcaggcatac caatcatcag
     4381 cttcgccgcc tgacctaaat attttccggc ttttgccagt gaatcaaaca tcttggcctc
     4441 cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga acgccttatc cggcctacaa
     4501 atgctatcaa cgcgctcggt cagtcccctc gccccatcgg ggagagggtt agggtgagga
     4561 ggaaccctcg gcactatgcc aacattcgcc aacatccccc ctcactctga ctttagtgtg
     4621 cgccttttgc ctgcgccacg atctcctcga cattttccgg cattggctca tacggcgttt
     4681 ctttcgccgt tggcttcgga tctttcaatg ccgccagtgc cgacttaatc gagaacagcg
     4741 ccagaaccac cacgaccacc ataaagaaga tggttaaccc ggcatccaga cggttgttga
     4801 acaccagttg cgccagctgt gactcagtat actgcgacgg aatattgccg ctgtctatca
     4861 ttgcctggaa cttattagca atggccagga agccgacttt cgcatccggg ctaaacgctt
     4921 tctgccagcc tgcggtcagg gtacaaatca gcagccaggc cgttggtacc agcgccaccc
     4981 aggcgtaacg ttgacgtttc atcttgaaca acaccacggc acagagcatc agcgccatcc
     5041 ctgccagcat ctggttggca ataccaaaca gcggccacag agtgttaatg ccgcccagcg
     5101 gatcgaccac gccctgatgg aggaagtagc cccacgccag tacgcacagc gctgttgcca
     5161 gcaggttagc aggcagtgaa tcggtccgct tcaggccagg agacaccacg cccagcagat
     5221 cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac cgccgtcaga ataaacagag
     5281 cttcaaacag aatggcgaag tgataccaga acgccacatc catcatacca cccagcgcac
     5341 cgtgcaggat gtaagccatc cccaccgcca gcgtcggcgc accgcccgca cgggaaatga
     5401 tcgactgttc acccacttcg ctggcaatct ggttgagcgt atccggagta atcgcaaagc
     5461 cccagctact caccacctgc gcggcagaag cgaccacatc cgccgtccct gccggagcca
     5521 gcaccgccat cgggctgttc atggcgaagt acacgcccgg atcgatgata caggcggaaa
     5581 ccagtgccat aatcgccacg aaggattcca ttaacattcc accgtagccg ataaagcacg
     5641 cctgcccttc gttcgccagc atcttcggcg tggtgccgga agagatcagc gcatggaagc
     5701 ctgacaccgc gccacaggcg atggtgataa acaggaacgg gaacagatta ccggtccata
     5761 ccgggccagt gccatcgaca aatttggtca gcgcaggcat ggtcagcgtc ggacgcataa
     5821 tcaaaatgcc taccgccaga ccaacgatag tcccgatttt caggaaggta gagaggtagt
     5881 cacgcggggc cagcagtaac cacaccggca gcaccgccgc cacaaaaccg tagcccacca
     5941 gcatccaggt cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc gggctttctg
     6001 ccacccagcc gccagagata atggcaaaaa taaggaacac caggccaatc actgacactt
     6061 cgccaatacg ccccggacgc agatagcgca ggtagattcc cataaaaatc gccagcggaa
     6121 tggtgaacgc aacggtatac gttccccacg ggctatgagt cagggctttc accacgatca
     6181 tcgccagtac tgcaaggata atgaccatga tcataaagca ggctaccagc gcaatcaccc
     6241 cggcagttgg ccccatctct tctttgacca gctcacccag cgagcgaccg tcacggcgcg
     6301 tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc gagaaccacc ccggcgagca
     6361 gccagatcat ccccggcagg tagcccattt gcgctgccag caccggcccc accagcggcc
     6421 ctgctccggc aatggccgca aaatggtgac cgaacagcac tttcttgtcc gtcggcacat
     6481 agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca accgccagca
     6541 catttttggc gatatacagc ccataaaaac ggtaagcgat cagatagata cagaccgacg
     6601 ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt taaagcaatg tatcccagag
     6661 caaatgctcc cattacagag agcactgtcc agacgaggta tttccctgat ttgttcatag
     6721 ttgttatccg tgtgcgtgtc catagagatg ttacattttg tttctataac atctttgtac
     6781 gatttaacaa cacggtaacc atgtaaatcg gagttgaaac caatatttaa cttaacaatg
     6841 ttaactcgat cactccgtta accgagtttt cctgcaatct catttaattc tgtcgggctc
     6901 atcccaaaac tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt tcatcgaaaa
     6961 cgatgatttc ccagctctga ttttgccgcc caagatgcag cggctggcag acgccgcgta
     7021 ccttgccttc agacatcggg cgatgatggg tggcgttaag ttccgtaccc accacacact
     7081 gcccgtcacg ggtcatcata aatccggcca tcgatcccag cgtttcagcc agcgccgccg
     7141 acgcgccgcc atgcagcagg ccaaacggct gatgagtacg ggtatcaacc ggcatttcgg
     7201 cttccagcac atcatctccc agacgggtat acacaattcc cagatgcgcc accattgtgt
     7261 tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc atgcttatgc
     7321 ccccagcgtt gagccgccat cgaccacaat atcctgtagg gtgatatggc tggcgaggtc
     7381 agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt tccccagcgg
     7441 aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg cgtcatcgct
     7501 cacccacagc gtgcgttgca tatcggtgtc ggtggagcca ggcgaaacca cattacagcg
     7561 cacgccgcta accgccagtt ccagcccgac gctcaacgcc aggcttttca gcgccgcttt
     7621 cgatgcgcca taagcactca taccaatacg cggcgtgtgt gcggcgtcgg acgccacagt
     7681 gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg gtttgctgaa acaggttaaa
     7741 cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc tctttactga gctgatcggt
     7801 cgcgcccatg cgcaaaattc ccgccgcatt gaccagcacg tccagtcgct ccgtttcagc
     7861 taacagtcgc tgacatacct gcgcgacctg cgcagcatcg gcaacatcca tcacttcggt
     7921 cgcaaaggga tattgctcct gagcgaacgc ttgatcaaaa ccggtaactt tcgctcccgc
     7981 ttcaacaaac gccagcgccg tggcgtagcc gataccttta cctgcgccgg ttacccagac
     8041 atttttaccg ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca ccaggcgtcg
     8101 atggtcgggt ttttcgccag catgacaaag tcgatatcgc catgcacttt gcgccagcgc
     8161 gccgccagcg ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt gtcgtcatcg
     8221 aacggttcat cggactcgtc cagcaacggc aggataacct cacgcagcgc cgctttcctg
     8281 gcggggacag gtgctggcag taatccttca gtcatcacca cccggccaga ccgcccggcc
     8341 acatatttca gcgacatcaa atgctcgtca cggctgaaat cggccagcgc atccgccacc
     8401 ataaacggtt taatatcgcg cataaatgcg tcggttgcgg tggtcataca gccaatgtgg
     8461 gcatataccc cggtaataat cagctggtta cgtccactct ctttgagcat ttgctccagc
     8521 ggagaacgat gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc ggcatctggc
     8581 gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg cccccacata
     8641 tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt gtaataaacc
     8701 gggatattgt gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat cacctgctcc
     8761 atcatcgggc agttctcgcc ccagaagctg acaaaatagt cctgcatatc atggattaac
     8821 aacgcggcac gttgcggttc aaaggcccag tcaactttat tctgcggaat atcgtgagac
     8881 tccggcagtg cgtaagcctg taattttgga atagccatcg tgttctctcc ttcaggctga
     8941 tgcgcgtgac gccagccact gacgtaattg ttttttatcg actttcccga ccgccgtcag
     9001 cggaagtgaa tccacacact ccacgcgatc cggtaattta aattcggcaa taccctgttc
     9061 acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct tttaccacca gataagcaca
     9121 gcttttttcg cccatcagct catcttccat gctcaccagt gcggcataga tcaccgccgg
     9181 atggcgcagc agcaggtttt cgatctcttc ggcagcgatc ttctcgccgc cacggttgat
     9241 ctgatctttc tcgcgcccct gcacggtgat gtaaccctct ggatcaatag agatcagatc
     9301 gccggaacag taaaaaccgt tggcatcaaa ggcactggca ttgtgctgcg gacttttgta
     9361 ataaccgcgg aaggtgtacg gcccgcgcgt catcaggcgt ccaacttccc cttgcggcag
     9421 tggatttcct tcagcatcag caacccatac ttcatcgtcc ggacacattg ggtaaccctg
     9481 ggtatggata attttctccg cgctatcatc aagtcgggtg tagttcacca gcccttccgc
     9541 catgccaaac acctgctgca actggcagcc aatctcagcg gaaatacgcg ccgcaagggt
     9601 ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc gaggcaagct gcgcccggct
     9661 ttcgccttca gccagcgcct gcaaccacag gctaactgcc ggcggcacca gcgcggtaac
     9721 gttaacctga tgttttgcaa tcaacgggaa gcaaagcgtg gcgctgggat cggccgccag
     9781 aacaaccgtt cctccggcaa gaaagacgcc cagcgatcct ggcgaactca tagcgtagtt
     9841 atgagccgcc gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac aaatctcgac
     9901 gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc gggatcagct tcggtgtacc
     9961 ggtggtgccg ccggaaagct ggaaataggc cacttcatca gcaggtgatg gcgtagcagt
    10021 aaaaccgtct gccggatggt taatcgcatc ctgcaagtta tgctcaccgc tgtcgttgag
    10081 cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa gtattgagga aatcatcccc
    10141 gctaaacagc gcatgttggc gatcggcaat cagtaacgcc ggttcaatct ggctggcata
    10201 ggcgttcagt tcactacgct gatggctgaa caacgccagc accggcgcaa cgcccagttt
    10261 cagcagtgcg aaaaaggtaa tgtaaagttc agcgacgtta cccagttgta ccagcgcggt
    10321 ttcaccgggt ttaatgccct gacggcgtaa actacacgcg aggttatccg ccgcctgatt
    10381 cagctcccgg taactcaact gtcgctcgcc gtcgataacc gcgatgctgt cactcgcagc
    10441 gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag tagccttttt cccgatagcg
    10501 acgggcaaac tcttccggcc agcgggtgaa tggaatgctc atcctcgctc cttaatgcaa
    10561 tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact cacccaacgg
    10621 tgacgacgca ggcacaatcc ccgctccggc aaacagacgc acctgatttt cccgcagctt
    10681 cgcgcagcgg atggtcacca cccattcgcc gttaccttcg ctgtcacacc aacccacaat
    10741 gccgccaaac agttcgcggt caaacggctc cagttcagca ataacctggg tcgcggcctg
    10801 atgcgggaaa ccgctcagcg cgggggtcgg atgcagcaga caggccagag tcagtgcgtt
    10861 ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga tgccacagcg tcggcgtggt
    10921 aatcaattgt ggagaggagg gaacgtgtaa ctcactactg cgttcgcgca gtacctcttt
    10981 catcgcctga gtcaccagtt catgttcatg gcgatctttt tctgacgcca gcagacgatt
    11041 acccgcttcg cgatcgagca cttcatccgg ctgacgacgc gcggaaccgg ctaacggaat
    11101 ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc gggctggccc ccagcaggac
    11161 gccaccatca gccagcggga catggaagtt gtaactaacc gggttttgcg caatcaaccg
    11221 ttccagcaat acgccactat caatggcggc gtcagtggtg atatcaatca atcgtgacaa
    11281 caccactttg tcgacctgcg gcgtggcggt aagtgcggcg gcgcgggcaa ccatctgttc
    11341 aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg ttcagcgact ggctgcgggt
    11401 aaaacagtgg gctgaggtct gtttttcctg acgggagaac gactgccagg attcgggaat
    11461 atacagcgac gaaggctgac gtggatcgaa gggaatcgcc ccgaccatca ccggattttt
    11521 gatgccctgc gctttggcat cggcaaacag cgcggcgagt ttttgctgga agggactgtc
    11581 gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg aaacatcctg acgtcgtaaa
    11641 actgcggtac ggcgacataa agaaaaagcg attgggcgca agtgttgcca tggtctgctg
    11701 tacttcctca gccagtgacg tatccatatc atcctccaca aaatgataaa ggctttaata
    11761 atgattatca tttatatttt cggtcgctaa cctaaaagca aacgcgcact atgtcaactc
    11821 ttgaggtaac gcaatctgca actcggggtt gcatctggtt gtggttacaa tgaaaatgag
    11881 aagcattatt gatgaattcg cataagcgca atgtgatgtc ctgcgccgtt ttgccccctc
    11941 tcccttccag ggtgagggct ggggtgaggg ttaatgttcg caccagtgct ggctgttccc
    12001 ctcaccctaa ccctctcccc aaaggggcga ggggacggat tgtgcgcttt gtcgaatttg
    12061 tcattacgcc cttaacctta ttaataacag gaagctgact tgtgagactc gccccgctct
    12121 accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgta gttcaggccg
    12181 ccgactggcc gcgtcagatt actgacagcc ggggcactca taccctggaa agccagccgc
    12241 agcgtattgt ttccaccagc gtcaccctga ccggatcact gctggcgatt gatgctccgg
    12301 tgatcgccag cggcgcgacc acgccgaata accgcgtcgc agatgaccag ggctttttac
    12361 gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc ggcgaaccga
    12421 gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca accggcgggg
    12481 attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta atcatcaatt
    12541 acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc gggcatgaga
    12601 aacaagcggc agagcggatt gcacagtttg ataagcaact ggcagcggcg aaagagcaaa
    12661 tcaaattacc gccgcagccg gtcactgcca ttgtctatac cgccgctgca cacagtgcca
    12721 atctctggac gccggaatca gcacaagggc agatgctgga acaactcggc tttacgctgg
    12781 cgaagttgcc cgcagggtta aacgccagcc aaagccaggg caaacgccat gacatcattc
    12841 agcttggtgg ggaaaatctg gctgctgggt taaacggtga atcgctattc ctgttcgccg
    12901 gtgatcagaa agacgccgat gcgatttatg ccaatccact gctcgcgcac ctgcctgcag
    12961 tacaaaacaa gcaggtttat acgctgggaa ccgagacgtt ccgcctggat tactacagcg
    13021 ccatgcaagt gctggacaga cttaattcac tgttaaaaaa agattgataa aacatacaat
    13081 tagagcacta ataatcccct cgcccctttg gggagagggt tagggtgagg ggaaaatgca
    13141 cctttagcta actgtcagac gctgtcacct gcggcggcgt ctggcgaaaa cgtcgcaact
    13201 ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg ctcgcgcttg
    13261 cggaagcaac cggtgtcatc atcgcgccca aaccacccaa cagcgccgcg cctatggcat
    13321 cgcccgtcac gttctgcgcc gtccacaaac cgttaatccg ccctaacatc gcttccgggg
    13381 tttgcgtttg cagcattgta tattgcagca acgaactaac cgcgctcagc cagccgaaca
    13441 gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga ccaatggcga
    13501 ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcacta tgtgccagct
    13561 tcccgctggt taacgcacca atagccgcac cgagcgggat cgccgcatag agaaaaccaa
    13621 tctgtgccgc cgacatctgc cagttgtcag ccagcgccgg atacagtacc cgcaccgcgc
    13681 tcgccatcgt caataaacca cccagcaacg caatccctcc taccagcggg ctggcgagca
    13741 gaaaacgaaa tcctgccagt aatgatttca acggatgctc gcgcggctgc ggtggcggtg
    13801 gcaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc gccgccagcc
    13861 cgtagttcca ggctacgccg ccggtcgcca gcaataaacc gccaatcatg ggcgaaatca
    13921 ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag ttttcacgcc
    13981 ctaccagtgc aggtgttgcc gccagtagcg ccgtaacacc aagtgatgcg aaaaaaccat
    14041 cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagc gcgttcaggc
    14101 acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact tttttgcgct
    14161 catagcgatc cgccagcaca ccgccaacca tcaggccaac aaacatcgcg ccgccggtca
    14221 gcgtcaccga aagccccacc tgccaggtag aatgcgtcat catctggatc tgcaccggca
    14281 ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg aatactgcgc
    14341 gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt ttattcatta
    14401 caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg ctaacatatc
    14461 caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat gtctggttct
    14521 gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat tatcgcgacg
    14581 gcattaagcc tgctcattgg ggcaaaatca ctccccgcgt cagtagtgct ggaggccttc
    14641 tcaggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcgact gccgcgcacc
    14701 cttgccggtt tacttgcagg cggtgcgctt gggcttgctg gggcgttaat gcaaaccctc
    14761 acccgaaatc cacttgccga ccccggcttg cttggcgtga acgccggagc cagctttgcc
    14821 attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact ggcgatggcc
    14881 ttcgccgggg cgctggtggc ctcattgatt gttgccttta tcggcagtca gggcggcggg
    14941 cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt gctggaaggg
    15001 ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg tttctggcaa
    15061 gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat cccggttctg
    15121 atcgccggag caactgcgct attactgagt cgcgcgctga acagtttgag cctcggcagt
    15181 gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttgattgg tctgctggcg
    15241 attaccgtgc tttgtggtag tgcgacggcg gtagttggcc cgattgcctt tatcggcctg
    15301 atgatgccgc acatggcgcg ctggctggta ggtgccgatc atcgctggtc gctgcccgtc
    15361 acgctacttg ctacccctgc cctgctgctg tttgccgata tcatcgggcg ggtgattgtg
    15421 cccggcgaac tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt gctgatcttc
    15481 ctcgtgcgac gtaaaacgcg aggtggtgca tgatttacgt ctctcgccga ttactcatca
    15541 cctgtttgct gctgatttcc gcctgtgtgg ttgcaggtat ctggggatta cgcagcggtg
    15601 ccgtcacgct ggaaatctcg caggtattcg ccgcgttgat gggcgatgcg ccgcgcagta
    15661 tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg ttgattggcg
    15721 cggcgctggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg ctcggcagcc
    15781 ctgacgtaat gggctttaac accggggcgt ggagcggcgt gctggtggcg atggtgctgt
    15841 ttggtcagga cctgacggct atcgcgctgg cagcaatggt gggcggcatt gtcacttctc
    15901 tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg attattatcg
    15961 gcatcggcgt tcgcgccatg ctggtggcct ttaatacttg gctgttgctg aaagcgtctt
    16021 tagaaacggc gctaacagcg ggtttgtgga atgccggatc gctcaatggc ctgacgtggg
    16081 caaaaacttc gccttccgca cccatcatca tattgatgct cattgccgcc gccttgctgg
    16141 tgcggcgaat gcgcttgctg gagatgggcg atgataccgc ctgcgcgctg ggcgtcagcg
    16201 tcgagcgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct gcggcaacgg
    16261 cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc cgacgcatta
    16321 gcggcaccgc tcgctggggg ctgacccagg cggcgctgtg cggagcgctg ttactgctgg
    16381 cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt ggcgtcgtta
    16441 ccgtcagcct cggcggtatt taccttatcg tcttgttaat tcaggagtct cgcaaaaaat
    16501 gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctgggatatg gcaaatatac
    16561 cgttgcggaa aatctgactg tagaaattcc tgatggtcac ttcacggcaa ttatcggacc
    16621 aaatggctgt ggtaaatcca cgttactgcg taccttaagc cgcctgatga cgcctgctca
    16681 cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag aagttgcacg
    16741 ccggattggt ctgttggcgc aaaatgctac cacgccgggc gatatcaccg tgcaggagct
    16801 ggtggcgcgt ggacgttatc cgcatcaacc gctgtttacc cgctggcgca aagaggatga
    16861 agaagcggta acgaaagcga tgcaggccac gagaataact catctggcag atcaaagcgt
    16921 ggataccctt tctggcggac aacgccagcg agcgtggatc gcgatggtgc tagcccagga
    16981 aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc atcagattga
    17041 tctactggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg cggcggtgct
    17101 gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc gggaagggaa
    17161 aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg agcgcattta
    17221 tggtctgcga tgcatgatca ttgacgatcc ggtagccgga acgccgcttg tggtgccgct
    17281 cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca tcatggcatc
    17341 ctgttttctg gatgccatcg catagcgcaa taacacacta ccacaagcca ccatcccgcc
    17401 gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt tcactggcaa
    17461 tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgacat ttgcttttgt
    17521 taattgctcg acaagatact gccgattacg taattcaccg ttcagttccg caacgtcagt
    17581 aaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag aaatagagaa
    17641 atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa ttcctgccgc
    17701 gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt gatttttcgt
    17761 tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgatct ccagtttatt
    17821 acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga tatacccgct
    17881 caaaacggtc tgcgcctctt tactggttgg cgcggtaaaa cttagcgtcc aggaggtata
    17941 cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca ttttttcgct
    18001 caatgcaaca atagcgcgat gcaaatccag ttcatcgatt ttcgcctctt ttaattggtc
    18061 catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaacagact gaaacttctt
    18121 gataaacagg ttaaatgcac cgccacggtc gatattaaca tcgagatcaa gaacgcggag
    18181 tttggtaaag gttttctcca ggtcctgcca ctgaatggct tcggcgggag taataacggc
    18241 tgagctggtc catttttgcg gcaggatgaa agagatcagt aaacctgcgc aggcaaatgc
    18301 aaaaacgacc gccatgaccg tttttttggc ccgccataaa acttcgatta gattaagtaa
    18361 atcaatttca ttattactgg gcgacgccag aggatactca gaaaaatgag cgtcacttcc
    18421 ctgtttaata ttcaatgatg acatggggta taacctgaaa atgagtccaa taggggaaaa
    18481 tctccacggc gctcacttta ccagttggtg agaattatcc gaagctgaag tttgtaaatt
    18541 ccttccactg aatgcaatat tcactctatt taagagaaat attcatcaaa cctataaatc
    18601 aaacaaataa aaaacccata attattttct gtaattatgg gtttatagat aatattaatt
    18661 tacctgttta gcgttgcgcg aataatcggc ccaattttta caaacgcccc tggagagata
    18721 atatccacat gcgcacaatc ctgacgatag atatccagct ccgcaatcca cggcgaccag
    18781 gcgcgttcag gactcatacc ttcctgaagc gtgcgttcag caacaaacaa cgtcgcttta
    18841 ccgtcaaacg gtacgctatg agcagtcgtc agcaggcgca cagcatcagc gtagttgcct
    18901 tcaatggtgg taaacaactc cgttgaagta cttccctgct gtgccgccag gaaggcctcg
    18961 cgctcgcggt taatctccgc cagcacttcc gggtccagac cattagcttc tttttcctgc
    19021 cagttttgcg tttctggcgg ccaggtatcc agcaagccaa gaaatgccac ctgttcgcca
    19081 cgggcacgca gtcgcgccgc aataccctgc gccagcgtac cgccaaggga ataccccagc
    19141 aggtaataag ggccgtgcgg ttgttgctca agtaacgttg ccagatgcgc ttcgcagact
    19201 tcatccaggt ttgtcgctgt ctgcatgggt ccatgagggc gcggcgactg aatgccaata
    19261 atcgaccatt gtggatcgag ataacgcgag agtacgctaa actgccaggc aaaaccggac
    19321 gccggatgga aacaaaacag cgtcgggcca ttaccttcac gcaacggcag aatggtttcg
    19381 aatcccatgc gccgggagct gtcctcttca ccatcaataa tcgttgccag tttggcgacg
    19441 gttgacgcga ccatcacctg ccccggcgtc acctgacgag caaactgtcg acttaactgc
    19501 gctgccagtt tcattgccag tagcgaatga ccgcccagcg caaagaaatc agcatcggca
    19561 tcctgcacgt cacaacccag caacgacgcg aatgccgcgg cgataatcgt ttcactgccc
    19621 gctttcggtg cacgtcccga cgcttgtgtc ttcagttcag gcaacggtaa ggctttgcga
    19681 tccagcttgc cgttggcgct aagaggtaac tgtggaagtt gcagcagaac caccggtacc
    19741 atatgtggtg gcaatgtttc acgaagctgc gcctgcaatg cgctggtatc caacggcagg
    19801 cccgattgcg acaccagata gcccaccaac tgacgcgcat caccaccagt ggctgccgcc
    19861 tggttaatca cacaggcgtg ggtaacggct tgttcgacat ccggcagcgc ctgcatcacg
    19921 cgatcgattt cgcccagttc gatacgctgc ccgcgaattt ttagctgatc gtcactgcgc
    19981 ccgaggtact ccaccgcgcc gttatccagc cagcgggcaa cgtctccggt acggtacatc
    20041 cgttcgccag gagcaaaagg atcggcaata aagcggctgg cggtcagatc ggggcgtccg
    20101 agatagccct gcgccagttg aatgccggta agatagagat cacccgccac acccggcggc
    20161 accggatgca tcatcgcatc aagaatacgc agccccatgt tccacaccgg ataaccaatc
    20221 ggcacactgc tgccgcgcac ctgtgccagt tcctcgccaa aagccggata ccagctgaca
    20281 tctaccgccg cttccgtcgg gccatacaga ttatgtaacg gcgcaccggt taactgttgc
    20341 cattcacggc ataaatcagc cggtaaggct tcaccactac agaaaacctg tttcaacgtc
    20401 gcgcaactct ggcgagcggt ttgcggagtc agcgaggcaa caaatgccgc cagcatcgac
    20461 ggcacaaagt gcgtggtcgt tacgccatat tcggcaaaga attgctgcat agcgagcggg
    20521 tcacggtgcg cttccggttc agccatcacc agttttgccc ctgcgataaa cggccagaaa
    20581 aactcccaca ccgagacatc aaaactgcac ggcgtttttt gggcaacgac atcttcgcct
    20641 gtaagcgggt agtgattttg catccataac aggcggttaa cgatagccgt ctgcccgacc
    20701 attacccctt tcggcctgcc ggtggagcca gaggtaaaga tgatataagc cgtgtggtgc
    20761 ggttgtgaaa gttgcagcgg cgcactgccc tggggggtaa gcggggcgtt atagcaaagg
    20821 cttgttaaat tgggaacatc gctaaagcgc ggcagttgat cgtcggtggt aattaacagc
    20881 gacggacgcg catcttccag catcattttc aggcgatcgt ccggatagcc ggtatccagc
    20941 ggtagccagg ccgcgccagc ttcaactatt gcatggagtg ccagggtcaa aaagaccgag
    21001 cgcggtagtg ccaccgccac gctgtcccct ggtttaacgc cgcgctcacg cagcagattc
    21061 gccagcgcca ccacctgctc gcgcatttcc cgatagctga acaggtaacg cgcatctgcc
    21121 agcgccggag catccggtgt ttttgccgct tgttctgcca ccagcgcgct cagcgtggtt
    21181 tctggaatct caacctgagt ggcgttgatc tgcgccagct gcgcatactc acctggcagc
    21241 ataatatcga catcgccgca caacagcgcc ggatccgcgg cgaactgggc aatcagcatt
    21301 ttcaggcgtt cagcatgctg gattaacgtt ggctcatcgt aacgctgttt attggcgagg
    21361 atctcaatac tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag gtcattaacc
    21421 ggaccggttg ccagggtatg ggtttgcgcc tgaacaccag gaatatccag ttggtaatca
    21481 aataccttaa tattgagtac cggaccaaac agcggttcat cacctgccgc tcgcccgctg
    21541 tcacggacaa tttgttcggc atcgtaacgt tgatggcgac gcattttttt cagttgagcc
    21601 gccagtcggg ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat acccaacggt
    21661 aaaacgttga gcacgggtcc gctagccgtc agcgccgccg agcccagtcg acgcataaag
    21721 ataaatccgg cggcatagtc catgcgattg cacaatcgcc ccagccacaa ggctgccagc
    21781 gcaagggcta aatcggtacg ctgcacacct gaaagttgcg tagccagctg gcggaattcc
    21841 ccgtcggtaa attccagttt caggcgcaga atatctgccg aagcgctgcg ccccggtaaa
    21901 ggtgccggag aaagtgacgc gggcggcggc agttgacgac gctgttctgc ccagaatgcc
    21961 gcatcacgct gccaggcttc gctttcgcgg tattgctggt actcttccac tacatcagcg
    22021 aaaggcgtaa acggcgaagc aggcgttggt tcgccacgca gccatgtgca gtaaatattg
    22081 gcgatctggc gagtaatggc cgggaaactg aagccatcga ccagcaaatg gtgataacgc
    22141 tgataccagt accagcggtt atccgcaaca tgtatcagct gatgaaagac cagtggttta
    22201 ccgctatcga cgcgcaaatc ttgttgcaaa tccgccagca ttaatgcctg cgcagtaccg
    22261 tgcggatcaa tattggttcg caggtcgata atttctggct gttcgaatat cagcgcatca
    22321 tcgacccact gccagacttc gccgttatct tccgtaaaac gcatccgcaa cgtatctgct
    22381 tgcgctagtc cggcaaccac cgcgcgggcc agtaatggcg catcaacatc tccagttaac
    22441 tcaacgtaat gcgccacgct ccaggcggag ggtaaatccg acagtttttc tgccatccag
    22501 atgccgggct gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc tgcaactggg
    22561 tgaaattggc cggtgtcaga gtacgccagt ggacttccag ccactgctgg caggacgcct
    22621 gtgactgcgg ctgacacaca atgtcccagc ctgccgataa gaggcattgt tgcggccaca
    22681 gactgaattg cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgcgga tcatcgaagg
    22741 gattactgaa tgccatactc aactcctgtc atggaaaagt ggttgccaga ggtcgattaa
    22801 cccctgcatc aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa cctgacgcca
    22861 gaaaatggat tcttttatag ggtgcaattg cgcatacagc gcctgattgg cccgcatgat
    22921 catcggctcg cgaatacccg cttccagcac aatgcgcaga ccttcggcgc taacttcacc
    22981 agcttttagc ttttcaagta acacgccctc ttgctgcccg tcccgatgcg gccaccagta
    23041 cgatcctgac tggcttaata cacagccaaa gcgttcaggc cagtgcagtc cggcatacag
    23101 cgcggacagc ccaccaaaac tctgcccggc gaccacggtg cgatcggcgc gatcgctaaa
    23161 aggggcaata gctttcacca ggggtaataa ctcttgctgt actgccagcc agaaatccgc
    23221 attacacggc agttcgtggg cgcggtgcgt ggtgtcgata gcgtcgatca acacatacac
    23281 ggcgggagga agttgctgac gatgggtcag cgaagtcagc acaggccaga cgggcatact
    23341 ttgcgcccaa aattcgccat cgagcaaaac tgccagcgga cgttcttctg ctgttacatc
    23401 gccggtggta aaaatccata cacgacgtga atttttcaac cgttcacttt tccagataat
    23461 ttctttggct gctatttctg gcacttgcgg acaatcccat cccggttgca gaggcgcttg
    23521 cggcatttcg agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt gtggattcag
    23581 cggatcggct attgcctggg gtaatagttt tcgccagcct tcacgtaatt cgaggcgatc
    23641 gggggatggt gcagaaaaaa tgtcatcgcg ttcggtggga ataaagcagt agctgccgcg
    23701 ccagttggca ttgagttgtg tcgtccactg ccagacatca gtacctgcaa ttcgctgcat
    23761 cgactggggc tggctgttct gatgatgatc ggtcacacca gtgatgtaga tccatacgcg
    23821 ctttatcgtc gagtattctt cagaaccttg gggatcacgc caccagaaag tgacctcaaa
    23881 catttcgtca ttcagacgct gccattccgg gccatgtttc gactgccacc agctctcact
    23941 tcctactttt aacgccgtca caccataacc ccatgtttac tgtgcaattt ttcattgatt
    24001 gcagaaatat attgataata ttattgataa ccatttgcat ttgcaatagc gtaatggcgc
    24061 gccgtgggaa gcgcggacat ttattaacca actgcactgc gtgtctttca ggatcaaagg
    24121 ttttcgcggt agcgggatgc gtcgtgttga ggacgaccat gcccgacagt tgcaattcgt
    24181 ggcaaaaatg caggaataaa acaatgaaca agaagattca ttccctggcc ttgttggtca
    24241 atctggggat ttatggggta gcgcaggcac aagagccgac cgatactcct gtttcacatg
    24301 acgataccat tgtcgttacc gccgccgagc agaacttaca ggcgcctggc gtttcgacca
    24361 tcaccgcaga tgaaatccgc aaaaaccccg ttgcccgcga tgtgtcggag atcatccgta
    24421 ccatgccagg cgttaacctg accggtaact ccaccagtgg tcagcgtggg aataaccgac
    24481 agattgatat tcgcggtatg ggtccggaaa acacgctgat tttgattgac ggcaagccgg
    24541 taagcagccg taactcggtg cgtcagggct ggcgtggcga gcgcgatacc cgtggtgata
    24601 cctcctgggt gccacctgaa atgattgaac gtattgaagt tctgcgtggt ccggcagctg
    24661 cgcgttatgg caacggcgcg gcgggcggcg tggttaacat cattaccaaa aaaggcagcg
    24721 gtgagtggca cggctcctgg gacgcttatt tcaatgcacc agaacataaa gaggaaggtg
    24781 ccactaaacg caccaacttc agcctgaccg gtccgctggg cgacgaattc agcttccgct
    24841 tgtatggcaa cctcgacaaa acccaggctg acgcgtggga tatcaaccag ggccatcagt
    24901 ccgcgcgtgc cggaacgtat gccacgacgt taccagccgg gcgcgaaggg gtgatcaaca
    24961 aagatattaa tggcgtggtg cgctgggatt tcgcgccatt gcaatcgctg gaactggaag
    25021 caggttacag ccgccagggc aacctgtatg cgggcgacac ccagaacacc aactccgatt
    25081 cctatacccg ctcgaaatat ggcgatgaaa ccaaccgtct gtatcgccag aactactcgc
    25141 tgacctggaa cggcggctgg gataacggag tgaccaccag caactgggtg cagtatgaac
    25201 acacccgtaa ctcgcgtatt ccggaaggtc tggcaggcgg taccgaaggg aaatttaacg
    25261 aaaaagcgac acaggatttc gtcgatatcg atcttgatga cgtgatgctg cacagcgaag
    25321 ttaacctgcc gattgatttc ctcgttaacc aaacgctgac gctgggtacg gagtggaatc
    25381 agcaacggat gaaggacttg agttccaata cccaggcgct gaccggggcg aacaccggcg
    25441 gcgctattga tggtgtgagt gccaccgacc gtagcccgta ttcaaaagca gaaattttct
    25501 cgctgtttgc cgaaaacaac atggagctga ctgacagcac catcgtaacg ccggggctgc
    25561 gtttcgatca tcacagtatt gtcggcaata actggagccc ggcgctgaac atatcgcaag
    25621 gtttaggcga tgacttcacg ctcaaaatgg gcattgcccg tgcttataaa gcaccgagcc
    25681 tgtaccagac taacccgaac tacattctct acagtaaagg tcagggctgc tatgccagcg
    25741 cgggcggctg ctatcagcaa ggtaacgatg acctgaaagc agaaaccagc atcaacaaag
    25801 agattggtct ggagttcaaa cgcgacggct ggctggcggg cgtcacctgg ttccgtaacg
    25861 attatcgcaa taagattgaa gcgggctatg tggctgtagg gcaaaacgca gtcggcaccg
    25921 atctctatca gtgggataac gtgccgaaag cagtggttga aggtctggaa ggatcgttaa
    25981 acgtaccagt tagcgaaatg gtgatgtgga ccaataacat cacttatatg ctgaagagtg
    26041 aaaacaaaac cacgggcgac cgtttgtcga tcatcccgga gtacacgttg aactcaacgc
    26101 tgagctggca ggcgcgggaa gatttgtcga tgcaaacgac cttcacctgg tacggtaagc
    26161 agcagccgaa aaaatacaac tacaaaggtc agccagcggt tggcccggaa accaaagaaa
    26221 tcagtcctta cagcattgtg ggtctgagcg cgacctggga tgtgacgaag aatgtcagtc
    26281 tgaccggcgg cgtggacaac ctgttcgaca aacgtttgtg gcgtgcgggt aatgcccaga
    26341 ccacgggcga tctggcaggg gccaactata tcgcgggtgc cggggcgtat acctataacg
    26401 agccgggacg tacgtggtac atgagcgtaa acactcactt ctgatgctaa cgtcagattg
    26461 ttgacaaagt gcgcgtcgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    26521 aatcttgcca attcaatata ttgcaggacc atgtaggcct gataagcgta gcgcatcagg
    26581 cagttttgcg tttgtcatca gtctcgaata tggtcgatat gaaaactacg catacctccc
    26641 tcccctttgc cggacatacg ctgcattttg ttgagttcga tccggcgaat ttttgtgagc
    26701 aggatttact ctggctgccg cactacgcac aattgcaaca cgctggacgt aaacgtaaaa
    26761 cagagcattt agccggacgg atcgctgctg tttatgcctt acgggaatat ggctataaat
    26821 gtgtgcccgc aatcggcgag ctacgccaac ctgtctggcc tgcggaggta tacggcagta
    26881 ttagccactg tgggactacg gcattagccg tggtatctcg tcaaccgatt ggcattgata
    26941 tagaagaaat tttttctgta caaaccgcaa gagaattgac agacaatatt attacaccag
    27001 cagaacacga gcgactcgcg gactgtggtt tagccttttc tctggcgctg acactggcat
    27061 tttccgccaa agagagcgca tttaaggcat cggagagaca aactgaagca ggttttctgg
    27121 attatcagat aattagctgg aataaacagc aggtcatcat tcatcgtgag aatgagatgt
    27181 ttgctgtgca ctggcagata aaagaaaaaa tagtcataac tctgtgccaa cacgattaat
    27241 tgacaacatc tggtacgatt cgcccgcagc catcactgac tgcgggcgaa agtgtaaagc
    27301 aggtgcctta ccactctgac ctgacaaccg gatatgcagg gattgctccc cgcacaacgg
    27361 ctacttcttc ggttcgtaag cgagaacagc cttaaactca atattacgtt cctttacggt
    27421 gaactcacac agcgagtctc cgaccagaag cgtaaatccc agcaccgtca aacacagcac
    27481 tatgactgcc acaagggcat atttcgtcag catgtttata ttgcctccat gcgaagaagg
    27541 gactaccatc cgagttgttg aggttcggag tggcagcccc aggattgata gaaatatctc
    27601 ctggggcttc atctttccag acctcagagt aaacctgaaa ccggaaagct tcaggcaccc
    27661 gccgttatct tacccttcct cagcaaaata tctgtttatt tatacagtaa gtcctgtttc
    27721 ggacttctgt tggcaacact cgttattgct cgtaaaacga aatcgcccgc agccatcact
    27781 gaccgcgggc gaaagtgtaa agcaggtgcc ctaccatcct gacctgacaa ccggatatgc
    27841 ggggattact ccccgcacaa cggctacttc ttcggttcgt aagcgagaac agccttaaac
    27901 tcaatattac gttccttcac cgtaaactca cacagcgagt ctccgaccag aagcgtaaat
    27961 cccagcaccg tcaaacacag cactatgact gccgcaaggg catatttcgt cagcatgttt
    28021 atattgcctc catgcgaaga agggactacc atccaaattg ttcgggtttg gagtggcagc
    28081 cccagg
//
LOCUS       DAJTYZ010000046        27785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000046 DAJTYZ010000000
VERSION     DAJTYZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27785
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.046"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(67..1359)
                     /gene="spaL"
                     /locus_tag="OO596_001877"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             complement(67..1359)
                     /gene="spaL"
                     /locus_tag="OO596_001877"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6717742.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKNRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            complement(1363..1764)
                     /gene="spa15"
                     /locus_tag="OO596_001878"
                     /gene_synonym="spaK"
     CDS             complement(1363..1764)
                     /gene="spa15"
                     /locus_tag="OO596_001878"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6717743.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            complement(1777..3837)
                     /gene="mxiA"
                     /locus_tag="OO596_001879"
                     /gene_synonym="virH"
     CDS             complement(1777..3837)
                     /gene="mxiA"
                     /locus_tag="OO596_001879"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6717744.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            complement(3850..4917)
                     /gene="mxiC"
                     /locus_tag="OO596_001880"
     CDS             complement(3850..4917)
                     /gene="mxiC"
                     /locus_tag="OO596_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6717745.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            complement(4936..6636)
                     /gene="mxiD"
                     /locus_tag="OO596_001881"
     CDS             complement(4936..6636)
                     /gene="mxiD"
                     /locus_tag="OO596_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6717746.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFDRFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            complement(6623..7255)
                     /gene="mxiE"
                     /locus_tag="OO596_001882"
     CDS             complement(6623..7255)
                     /gene="mxiE"
                     /locus_tag="OO596_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6717747.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            complement(7385..7813)
                     /gene="mxiM"
                     /locus_tag="OO596_001883"
     CDS             complement(7385..7813)
                     /gene="mxiM"
                     /locus_tag="OO596_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6717748.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(7797..8204)
                     /gene="mxiM"
                     /locus_tag="OO596_001884"
     CDS             complement(7797..8204)
                     /gene="mxiM"
                     /locus_tag="OO596_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6717749.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(8197..8892)
                     /gene="mxiN"
                     /locus_tag="OO596_001885"
     CDS             complement(8197..8892)
                     /gene="mxiN"
                     /locus_tag="OO596_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6717750.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(8834..9361)
                     /locus_tag="OO596_001886"
     CDS             complement(8834..9361)
                     /locus_tag="OO596_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6717751.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(9358..10083)
                     /gene="mxiJ"
                     /locus_tag="OO596_001887"
     CDS             complement(9358..10083)
                     /gene="mxiJ"
                     /locus_tag="OO596_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6717752.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(10089..10382)
                     /gene="mxiI"
                     /locus_tag="OO596_001888"
     CDS             complement(10089..10382)
                     /gene="mxiI"
                     /locus_tag="OO596_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6717753.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(10395..10646)
                     /gene="mxiH"
                     /locus_tag="OO596_001889"
     CDS             complement(10395..10646)
                     /gene="mxiH"
                     /locus_tag="OO596_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6717754.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(10654..11769)
                     /gene="mxiG"
                     /locus_tag="OO596_001890"
     CDS             complement(10654..11769)
                     /gene="mxiG"
                     /locus_tag="OO596_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6717755.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(11778..12236)
                     /gene="ipgF"
                     /locus_tag="OO596_001891"
     CDS             complement(11778..12236)
                     /gene="ipgF"
                     /locus_tag="OO596_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6717756.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(12236..12598)
                     /gene="ipgE"
                     /locus_tag="OO596_001892"
     CDS             complement(12236..12598)
                     /gene="ipgE"
                     /locus_tag="OO596_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6717757.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(12608..14224)
                     /locus_tag="OO596_001893"
     CDS             complement(12608..14224)
                     /locus_tag="OO596_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6717758.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEVIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVNKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDSKIWNMVKGYSSFV"
     gene            14538..16022
                     /gene="icsB"
                     /locus_tag="OO596_001894"
     CDS             14538..16022
                     /gene="icsB"
                     /locus_tag="OO596_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6717759.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEE
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            16035..16424
                     /gene="ipgA"
                     /locus_tag="OO596_001895"
     CDS             16035..16424
                     /gene="ipgA"
                     /locus_tag="OO596_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6717760.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            16440..17066
                     /gene="ipgB1"
                     /locus_tag="OO596_001896"
     CDS             16440..17066
                     /gene="ipgB1"
                     /locus_tag="OO596_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6717761.1"
                     /translation="MQILNKILPQVEFAIPRPSFNSLSYNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDNVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSNMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            17124..17591
                     /gene="ipgC"
                     /locus_tag="OO596_001897"
     CDS             17124..17591
                     /gene="ipgC"
                     /locus_tag="OO596_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6717762.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            17597..19339
                     /gene="ipaB"
                     /locus_tag="OO596_001898"
     CDS             17597..19339
                     /gene="ipaB"
                     /locus_tag="OO596_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6717763.1"
                     /translation="MHNVSTTTTGLSLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANKNINTTNSHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPDSPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            19359..20450
                     /gene="ipaC"
                     /locus_tag="OO596_001899"
     CDS             19359..20450
                     /gene="ipaC"
                     /locus_tag="OO596_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6717764.1"
                     /translation="MEIQNTKSTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMDTSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSTASQIAGNIRA"
     gene            20501..21499
                     /gene="ipaD"
                     /locus_tag="OO596_001900"
     CDS             20501..21499
                     /gene="ipaD"
                     /locus_tag="OO596_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6717765.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKKKYEDKPLYPATNTV
                     SQKEADKWLTELGGTIGKVSKKNGGYVVNINMTPIDNMLKSLNNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            21508..23409
                     /gene="ipaA"
                     /locus_tag="OO596_001901"
     CDS             21508..23409
                     /gene="ipaA"
                     /locus_tag="OO596_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6717766.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKESFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFFSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            23413..23649
                     /locus_tag="OO596_001902"
     CDS             23413..23649
                     /locus_tag="OO596_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6717767.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            23866..24795
                     /gene="virB"
                     /locus_tag="OO596_001903"
     CDS             23866..24795
                     /gene="virB"
                     /locus_tag="OO596_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6717768.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(25741..26520)
                     /gene="ipaJ"
                     /locus_tag="OO596_001904"
     CDS             complement(25741..26520)
                     /gene="ipaJ"
                     /locus_tag="OO596_001904"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6717769.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(27120..27257)
                     /locus_tag="OO596_001905"
     CDS             complement(27120..27257)
                     /locus_tag="OO596_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717770.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
BASE COUNT         9443 a         5650 c         4048 g         8644 t
ORIGIN      
        1 agtgttaata tgtgtgcttc aattaattta acagaccgtt gatatttatc tttgattttt
       61 aaaaccttat ctaattgttt caccaataag ctccattgtc tgctcataag taaaaccgag
      121 tcgataatct tgcttcagaa aactctcaac aacagatatt ttgttgtaga ttttatcctg
      181 agaggcattc tcccccggtt tgtattcacc aaagtcaata attgtcctta gctcttctat
      241 ttcagaaagt agctcccgaa atgcagcggc cataatacga tgtttttcat caacaacctg
      301 tgtaaatacc ctgcttatac tttttaagga atcaattgca ggaaattgtc ctttttgggc
      361 tagatttctg ctcaaatata tatgtccatc taaaatggat cttacctctt cagctaatgg
      421 atcagcaaaa tcatcatcct ccaaaagaac agtgtaaaat gcagtaatag agccacctgc
      481 ctttaacttt cctggccttt caagaagtct gggtaagcta tcaaaaaccg aaaccggata
      541 gcctcttctg gcaggtgatt ctccagcggc taaggccaca tcacgtaatg ctctggcata
      601 ccttgttaat gaatcaataa aaagcgctac tttatgtcct tcagtcctaa aaaattcggc
      661 tatagcagtg gctatatatg cagcattaca acgatcaacc gaagagtaat ccgaagttgc
      721 atatactaaa acacacctgt tttttttctc agagtttttc aaataatcaa ccgtttctgt
      781 aacctctcga cctcgctcac caattaaccc aataacatat atatcagcac cactatgttc
      841 aatgagcata ttcatgagaa aagttttgcc acaaccagct gacgcaaaaa tccccattcg
      901 ctgtccttca ccacacgtga gtaaagaatc aataacctta atacctgtta aaaaaggctt
      961 ctcaattgca gccctttcac tatatagcgg aggagcatta tctacaggtc gataaagaat
     1021 ttcactgtta tccgtaacag caaacttatc agtaacctcc cctaaaggat tgactactgc
     1081 tcccaataat ccacggccga cttgcgtatg aagaaactgg gcagtgggct taatcaacgt
     1141 ttgccgtgaa agtccacgag aatttccaat caagcttaat attgtttttt catcatgaaa
     1201 tcctacaacc tgagctcttg caacaatttc attactttca attccagcct gaatgttaca
     1261 aatctcacca atcgaaacat cgctaagact tgtttccaag attggccccg agattctatt
     1321 aggaaaagat aattgagtga gcaattttgt atagctcatt gattataaga cctcatttaa
     1381 gatttccatc ctctgataaa actcatgcag aatctctgca aaaacaatcc catcgtgaac
     1441 gtagtcatct ttaataacaa ctcttaactg taagtactca tcagaacgat gaagctctac
     1501 cagctcatta atgctataac taaagttcat taacattaaa tttaatatgt tataagcaga
     1561 tgactgaagt tttacatcac ttggtgcatc aaaattagcc cataacataa cctgctcatt
     1621 gacaagagct atattaatag caggcatact atccaaagaa atggtaatcg ctgaatggct
     1681 gtctaaatct gtaataattg atggaggaca accaatcgtg aaaagactat ctctaactaa
     1741 ttgaactaaa ttaatgttac tcatatttaa acctcactaa atagtcttta atacattaat
     1801 ggtatatgct tcatcaatct cagcatacga tataacgagt atactcttaa atctgttatc
     1861 tataagtctt ttaacaaacc tacgtatatc tactgatacc aaaagaacga aatttttttt
     1921 tgcatttctc aattctctca aagcatgtgc taaagtttcc attacctcat ccgaaacctc
     1981 tatatccata ttcaagaaag agccaccaga ggtttgcctt atcccttttc ttattgcatc
     2041 ctcaatatat ccggaaagca tcacaacttt aatctcacca gaaacagcta ttttactaca
     2101 aatataccta gaaagtgatg cacggacatg ttcaactaat gttatgacat ctttttctct
     2161 gggagcccaa agcgccaaag actccataat aagttttaaa ttgcgaacag aaatattttc
     2221 tccaagcaat ctttgtaata cctcagaaat tctctgtata gtcacatgtc ggaagacttc
     2281 ctttaataga tcaggatacc gattttcaaa ctgatctaac atattttttg tttcttgtat
     2341 accaaaaatc tcatttatgt tgtttaataa agcttgtgac aactggtgat aaaattcatc
     2401 ttgagcactt ttaattttag catcaatatt agttagattt tctgtatatg aaacgtctac
     2461 ccaagaaata acacgttcat tatatgaagt tgaaacaaca ggaataccta gagcatctat
     2521 atctccattt tcatctgtaa tacacacttt gtcaaaatat atattaaaac tatcggcacg
     2581 cacctcattt attaacaaaa caatatcatc gaccttaagc tcattgcttg ttctatataa
     2641 aatagttgga agcctgacac cataatcaat aaagaactga cttcttatcc tttcaataag
     2701 accttccata tcatttgcat ttatcttatt ttcggcaaat aataaaatta aaggaacggt
     2761 ttctgaacta atagcatcca gattttctat cattgccaaa gatgagtcat ggctattatc
     2821 aatatcaaat gtaccagtat agccactaga atcagactca gacagacttt tttctttttc
     2881 tacgaccttt ttataataga ataaagccgt caaagtaact gctatcagga aaaaaacaaa
     2941 aaacggaaaa cctggcaaca tccctatcgc caaggcaaga gctgatgtaa caatgagaac
     3001 aaaaggattc ccaaatatct gggacattat gttacgtccc atattatcgc tatcaccatt
     3061 aactcttgta acgataaatc ctgcacttat agaaattaac agagcaggaa tctgagagac
     3121 caatccgtca ccaatagtta gtatggtata agtagataat gcaccagata gggacattcc
     3181 atgttgactc ataccaacag aaatacctcc tattaaattg acaaaaataa taatgatgcc
     3241 agcaattgca tcacctttaa tgaacttcat tgctccatca aatgaaccat acaattgact
     3301 ctcacgttcc agaatactgc gtctttcttt agctcctgcg gcatcaataa ttccagcctt
     3361 taaatccgca tcaatgctca tttgtttccc cggcataccg tcaagagaaa agcgagcagc
     3421 tacctccgca accctttcag atccttttgt tataacaatg aactgcacaa cagttactat
     3481 tgaaaaaata acaaaaccta cagccagact atcaccaatg acaaactggc caaatgtagt
     3541 aataatttta cccgcatccg catcaactaa aattagtcga ctagtactta ttgataaagc
     3601 gagacgaaat aatgtcgtta tcaacaaaac cgaagggaat gtagaaaaac tcaatattct
     3661 ttcaatataa aatgatccca taaaaacgag aatagcaagt acaatattaa gccctattaa
     3721 aaaatcaaca aggtatgtag gtaatggtat gatcaacata gcaatgatca taaccataag
     3781 cactaatata attaattcag gcttagtact tacttgctta agaaaagact ggatcacttt
     3841 catctcctgt tatctagaaa gctctttctt gtatgcactt gtgacaagct ctcgtagaat
     3901 attgatcgca atttctcttt cactttcact tttaaatata tcatgaggac tttcattaac
     3961 agaagataga tatgtttgat acactacaga atcctcatga ctggctcgtg atacttctat
     4021 aacagaagta agaatttgct cactttcact tggatattga aaaatatata ataaagttag
     4081 caatagctgc tctgcactta aactgccatc ttttagcaag cttgaggatt ccagtttctt
     4141 cataaaaatt acgtctgaag ttctaatcat cttaattgca attaatttag ataacacaaa
     4201 accaaactca taagcattac agctcggttt ctcagaatcc atgtctacaa tcagtgactg
     4261 ctctacaaaa ttcacaattg tatgtctgtg attaaaacca aaattaccaa gccattctat
     4321 atactgatct gttgttgata tgttccccat gataaaacca cgataacatg cacgcaaaag
     4381 ctgtggttca agtttcattt tgttgccaaa taatttagcc tgtatagctg aatttattcc
     4441 agcattaaca cttttttcac tacctgatat tatctcatta ataattttag ttaatgattc
     4501 gacttgttca gcagtaagat ctttttcatt caaaagttcc cttaatgcta agacttgatc
     4561 acttggatct ttaaaatatc tctttagtct gtctataaaa tcccgatcta gaggaaggtt
     4621 atcttttaac gttctcttta aatcaaaaat cttgtgatta atttcatctt cttccccatc
     4681 taaaatacgt tcctgactat cactatttgt tcctttcagt ttctcaaggt ctcttctgtt
     4741 tataaatgac gaaagagcgg aagccatctc atcagatgag tcaatatact tgctattagc
     4801 caagccagaa tcaagctctg catcagcagt ctcatctcca tcatctgaat ttgtcattgc
     4861 attcaactta tcaatgaatg cagagctaaa aactcctgta tttttaacat caagcataca
     4921 tcacctactt tatagttagt aatttaagta tgaaaccaat gatttttcgt cttccagcaa
     4981 agtagtttca gatgggatta tttgtgtagt cttttgtatt tctctttcag atatgaggct
     5041 tttatattct gcagtattat aataacttga ctcctttatc tcacggggtt gaatcagaaa
     5101 aacccttaca atattactga tattacttgt tttatacttg aacacattac cgattacagg
     5161 aatagaggag agaaaaggaa tagatactat ttcgttagag ttcgtttcat gcgtataacc
     5221 acctatcaac aaacttttgc cttgtggaac tctcgctatt gtactgattt tggtccttcc
     5281 aacttctgga aggacagatg tattttcatt attataattg taatttgact gactatttcc
     5341 tgttccatcc tctatcgtta aagacatctc aatttgtccc cgagatgaaa agcgaggtat
     5401 aacatttatc aatgttccat acgtaacatg ctccaacgaa gaattgcgct caccaactaa
     5461 tgacacataa aaagtcctgt tattatcaaa tatagccgga atattctctt gtgtcaaaat
     5521 caccggcctt gaaactacat ttgctttctt tttctgattt aatgccataa ctgaagcaat
     5581 aaacttattg ccgtccaaag tgcttatcga tgctgatgag ctcatattaa atgacgcacc
     5641 aaacgaatca ccaaatgaag ccgttccttg ccaattaaca ccaaggttat ttaattcaga
     5701 tttatcaata tcgattatcc ataatgataa ttctatatgt cgtttggcta catctaactg
     5761 agtgataata tcgcgaataa tctgtatttg ctgatcatta cctttgacta atatagaatt
     5821 ggtttctgga tatgcaataa gtgatacatc ctctaatata gaactattgg taactgaact
     5881 aaatgaaaag tcattactat cctcagatac tttctgtgta atattaaagg gtggcatcgg
     5941 atcattttgg gcttgtctgt tactcagagc cttaccatta tttaataaac gttctacaac
     6001 agtagccacc cctgggatga caatatcctc ccctctcata ttataagttc tgtcactaac
     6061 aaatgtattt tttaatttta taaccccaaa attaacttta tctgtcccaa tagaagaaac
     6121 ctgcttatca agcaaagttg ctgtattagc taccagttca accaatgccg gaggaccaga
     6181 tatatagaat gttttatcac taatatttcc tcttatagga tatctgtgat cataaaggtt
     6241 agcatccttc agatactgaa taagataatt aagtgatata ttctccagca aaataacttt
     6301 gcttattaat tctccagagt catatatgta taatgcattt ccatctttat accatatgag
     6361 tcccacaagt aatgtcagtt tttcaagcat ttcttctgga ttagataaat caaactcccc
     6421 agatattctc ttttttgcag cttgtttgct aacaacaata ggataattta ataacgcaga
     6481 aaaacggtca aaaaaagatc ctaccgtatc actttgagca acatatttcg ctatgtcatt
     6541 ttgttctaat aaatgagagt caatattatt agcattaact atcaatggta acaatacaat
     6601 caagagagtc aaagatttaa tattaaattt tttcatttat ttttttcact aaaaaagtaa
     6661 tattcgataa ttctcttgca gaaaagccta gtcgtgtttt tatctcattt gaaaaatggg
     6721 atgttgatgc atacccatta ttcatggcag cagaagtgat tgtttgattg tgcaaaaata
     6781 catctaataa accatttact aaccgccaag tgttcagttg ttctttgact tttgccccta
     6841 aagcttttct acataaagat ctaaaataag cctccgacac accataatgc tctgtcagac
     6901 tctttattct tacctccttt tctttctcaa tagttctcag aagataaaga accaaatgat
     6961 atgactcagt cattcgtatc atagccagtg cattacttac attctggtca gatgaaaaga
     7021 tgaatcttaa cattaactta tcaagcatcg attcggtatt acaactatcc cgaagatcat
     7081 tctcatgata gaaatgaata catttttcag catggctatg ctctactaga gcaccactga
     7141 aatagtccca aaaagcaaga aattttgcac aactattaat gttaaagcga taatgatagt
     7201 tcacattatc actgaatttt atattttgat ttcggacaaa aaaaaaccct tccatgctgg
     7261 agccagctcg attatgttac gttctttatc ttttacaagc tcaacgttaa ctggttcatg
     7321 tccactagag taaatgtaaa acatattact tgtatttaga cctttatatt tactcacaat
     7381 acaattatat ccctcgaatt aattttaatt taacttcccc atccttctct ataaggatgg
     7441 tatcatttat cttattacct tttaaaacaa ccccgtcttt cagcatagaa aatagtttat
     7501 ctatagctat cttttgtgca tccatgaagc acccatctac ttccttaaca ttaaagttgc
     7561 ccatgaattt acctgcatta aaactataaa ccgccttacc actaatacat ttagagtaaa
     7621 catttatact tttattggtt gtattaaacg accataataa atcatttgga atagaaaaat
     7681 aatccttact tacaggaaca atatgccatt ctttttcaga attagatgat gactttaaag
     7741 cacacccact cagtgttaat agcaatatac ttaaaataaa tattttcaac ttattactac
     7801 catgtcgaat catctgcctc tctccctgct tcaaagacac taattaatga atcgaaggtt
     7861 gtagtcttaa tatagaaatc aatattgtta tatatggaca atgatgcata aaatccctca
     7921 tgcaaatcga catattgatt tgcatctatg agtttattaa gaagtgaact tttttgtgca
     7981 gctgtaagat aaggaaaacg tgaattgaat aattgttcaa tcttattgtg cagttcatca
     8041 ttatctatgg aatttaattc actctgtgag aaatattgca ttatttcata gattatatcc
     8101 tcatcaaatg attgcgaaga tgataatctc tcatttaaat taacaaattc ttcactattt
     8161 aatctttgct gcagagcatt gcttgcattt atttgattaa tcattaacag gattctcttt
     8221 taacaagtct tctgcaattt gtctcagttt cttatgtgct aactctttga aatatttttt
     8281 atcatttttt gttaattttt ccgcaagttc tattttaaca cctgatatta catcctgagg
     8341 actaaactcc actacttgat taccactaca gaatatataa ttattccctt cagaatattt
     8401 taaaataatc ttgacatctg agcgatattt atggagatca agttctaact ttttcctcag
     8461 tgctaaatta cacttgggca aaaccacttt taattctgtt actgtattac gcaacgaact
     8521 aagccactgc tccaataaag tgctaatgat cctctcatca tgtaaatttt ctgaaagcaa
     8581 actagttata tagtcctcaa tatttcttct gttttcattt tgctttaact tccattcaca
     8641 aatatagtca ataatatgct caaaagcagt ctgtatacca atttgataac catcacatac
     8701 agcatctata cgcagtgatt cagctttttt cgtagcatcc tttataacac aaattgcttt
     8761 cttcttactc tcagtgtctc tatcttttat ggttttacat aattcctttt caattctctt
     8821 tataacaaca ccatcatagg catgatgtct ggataccggt aaagtgcctt tttgcatatt
     8881 gcataccttc atttaatcgg gacaaatttc tttctacagg cgtataattt ccctccaaag
     8941 caattggatt aaataataag tttattcttt catatatacc aacagatata ttttccttcc
     9001 acgcaataag atgccaaaaa ccacttcgaa tagcggcctc cccttgctta ctgattttgc
     9061 ccccataata actaataacc atctcactat gattaattaa ccgagactct tgactaccga
     9121 taaaataagc aacagctgga agtaaattcc agttggcaac aaacatagcg gttaccccgc
     9181 ttaaaggttc aatatcgcaa tttagatcat atttattaat tatgagatta ttaatctcac
     9241 ttctgattac gccattttct aacagttcag aaggcaaatt caaccgattt ctatttatat
     9301 agaacgctgg atcaaaaatt attgaaagat attttttata aattccatcc attcttatca
     9361 tattttgttt ctcttgaacc accctgtttt gaatgcccat accaaaagaa tgacaactag
     9421 tacagccatc ccgagaaata aatatattac ttcatttgtt aaaaattccg atttaatttc
     9481 cttaacaggt tgtacatttg tataaacata ttcttctttc ggagttaata tgacagatat
     9541 attttcatac ttaacatcag aaaaggtgtt tttcaaaaat cgcttaatat tactaactaa
     9601 tagttcagac tctttcggtg agtcatatat agcgattact gatatatgca tcggttttga
     9661 agatatattt ttttcttcaa gatcatagct aacatgtatt tttgccgaaa taacaccacc
     9721 aatagaaact aaagactgtt ccagccgttg ctcaatagca ctatataaac gggccttttc
     9781 agctcttgga gaagacacta atgaatctgt aggaaacatt tgtgagatat caactctctc
     9841 cggatttggc aaatcgtaca tgcgcatcaa atcaactgcc gatgcaaatg tccccttttc
     9901 gacttgtacc gagatcccct gtttacctcc atcaactttt ctagcagtaa tattatggcg
     9961 ttctagcaca gatattattt catttgcctg tctttgagat aaattagaaa ttaactcttc
    10021 acgttgctca catccaatca acatcagcaa caagaataaa ataaaacctt tatacctaat
    10081 catataaatt aagactttaa taaagtttca acagccgaaa ctgtttttct tgctaacgtg
    10141 ccagctaatg atactcctat tgaataattt gagagcgtcg tctgtaactt ggccaaagat
    10201 tctgggttta atgattctgg attgcttacc atctccatta tttgtgatac ttgaatatct
    10261 gtatccatct taatatcact atatttagcc gacacgacat cttccagact tgatatctct
    10321 tgagattgaa aatcactggc tttgataata tcaacctgat tgactggata aatgtaattc
    10381 atgaatgctc cctattatct gaagttttga ataattgcag catcaacatc cttaatcact
    10441 ttcactgtat tggattgcgc gttcctatat aatgtatatt cagataattt actttggtat
    10501 tcagccagca actgtggatt cgaaggattt ttagctaatt tatctagtgc caatgttagt
    10561 tcaccttgta atgtttgagt tccatcatca aaagtttcag ataatgaact cagtgtccaa
    10621 tcatcattcg gtactgtaac actcatttta tcctcactta tttttatcta aaaaaaacca
    10681 gtgtttatca ttcaacatta cataactgtc tttgctgtta agatatgatt tacctttaaa
    10741 atcatcatct atcaaaagta cagaaaactc aatatactga tctccatata tatttttatg
    10801 ttcagaaatc agtctattaa tgagctcaag cttttcatct gtcagttctt ctctgacaga
    10861 atatgtaact ttattatttt cacaaatctc tttatactgg acatttacct ttgtgaaaat
    10921 acctttagca tccccccttg cattatgatc gctaagatag ttgaatttaa tatttttgat
    10981 atacggaaat tcaacaatca gagcattttt caatttatca agttcggtat cttttgaact
    11041 atttctttct ttacttaaaa acaccagtaa ttccgcttta tcactcacta aattcaacac
    11101 atacaaattg ataaatggga atcgttgatt gatatattgt tgaattctat ttatttcttt
    11161 gtttgacacc agaatatatc tgcatttggt aaatccggtt ttatttaaag ctacagatgc
    11221 ccataccgca tcattttgtg tttcggcaag aatataattg cactgtccag tgttgactat
    11281 tccatatctc ttatcatcta taatttcagc aatttctttt acttcatctt tctttaaaga
    11341 gagggaaaaa attataatta acacaacaat aacagcaaag aaaaaaaaca ccgagtggtt
    11401 cttatacatt ccgtttagta tatggcctct cgatttatcc tccctcatat ttttaaggtg
    11461 aaaagaaatc ccgtcatatt caaatgaatt aaattgtgaa ttgatatcaa tttgctcacc
    11521 tttttcatca ctaaattgac agatattatc tttgcttatg gtcaaataaa ttataccatc
    11581 cgatttacta tctgttacag gaataatatt ttctgggaaa ttgtcgtttc caaactcaag
    11641 tgtttctatc gtccgtccga gaacaattaa gttattccca taatataacg gaaactcatc
    11701 cccgacaccg tttgtgagtt tcactaataa cctgaatggt gcaagatttg agttctttgc
    11761 ctcagacata aatcctctta tatccttcga ttattctgct tgctctctgc tgctatccgc
    11821 aaatagcgtc tatagatatc ctctgcgtac tttaatcttt cttttttctt tttcggagac
    11881 gttcctgcat tataggctcc aacagcctcc caccctcttc catacatcat cataaactca
    11941 tttaataact ttgctgcata atgtacagaa agacatggat gactaattag catttcctca
    12001 gaatatccca tttctctaag tcttttagaa tgaaagtcat ttatttgcat tataccataa
    12061 tctttacttc cattgttatt aacattgact gcaaatttat taaatccgga ttctttttcc
    12121 gcaatcgctt ttaacaaact tgatggaata ttgtaccttt caccagcctt atcccaacaa
    12181 tcagctctcc ctaaatatgg gataaaacac agcaaaataa atacgaaacg ggacattaat
    12241 accccttcat tcttcgcgca aattcatcct tcagccaacg cacacgcgaa atataatatt
    12301 ctgtattaac atatacatcc tcgaattcat ttatcccggt aagatctaat cgcgcaatta
    12361 gatttccgcc ttcatcatca gtagctaagc atatcttttc tgagtaattt aggctcaacg
    12421 catatataag atcatttata ttttcaggca atgcacaaaa agggcataat aaatttattg
    12481 aatctccttc tttttcaata tatattagca catcatcatc aagcatgatt gcttgatcat
    12541 cgatatcaat taaagggata cccaaggctc ggcaaataac atctgctaaa tcttccatat
    12601 attcctctta tacaaatgac gaataccctt tcaccatatt ccatattttt gagtccccta
    12661 ttctttcaga ataagatagc tctaacgaag atagcggcaa ttttttcatg actttatttc
    12721 cgggcacacc agtattcatc tcttggattt ccatattacc actattcatt agaatagtag
    12781 aaaataatct tttctcttct gaggataatt tactatttaa ttgagaaaac tgacctgttt
    12841 catgttttct tattatttct cttttaattt cagcatcctg catacctgtt ctgtccttcc
    12901 cactcttaca attccagcaa ggtacagcac ctatagtata agccaataga gtcattcttt
    12961 gtgacaattt atacggctct ccattatcat ttttttgtaa cttcttgttt ataatctctt
    13021 ttatctggtt agccagatat atcacatcat tcttacatgg tggatttttt tctattgctt
    13081 ctgcagccca acctccaatc acaccatttt tgaggaaatt atcccccaac aaagaacata
    13141 ttgattcatc attaagcttg tcaacattcc tccagcctaa gcccattttt agcgctaatt
    13201 catttacacc aaagttaaat gtcaccactt ttagattaac gcttacttct ttcagaagac
    13261 catcactatt ccgaattaat aatttcgttg gctcccccct tttagagttt aaccctttta
    13321 atgcgttcac ctgatctttt aacatactct cctctccccc ggttaaactg gtcggcgtca
    13381 gaagagaagt cgaaacaatc tttaaatcta ctgttttacc agacaaagcc tgtgataata
    13441 actctggtct gctatataat gctgcgctta ctaactcctc agctttatta cgagcagcaa
    13501 cagctctttc agatgagttt tttttcaatc cataggctga tataactcca tgtctgatac
    13561 ccgaaaaaat ttccttcccc tcgtcatcca ccacttttga aagccacata ttcgcgatat
    13621 gatcactctc tctcgtagaa gcacaacaaa ttccttttcc attatactct ttgacaaata
    13681 tatttttatt ccctatcttc atatgagagg caggtgttaa ttgaaaacca taatttttcc
    13741 catgatgact aatattcttg ttgaccggtc cccaattctt attatttaaa taatcagtaa
    13801 atatttgttt tattttcgtt tgcaattcct ttttattttt aatgcctagc tgtgctctta
    13861 tctcttcact tattacttta ttaagctcat taatatgtct tttattaact tcaccaagtt
    13921 tataggtatc taatcgttcc atcatggctt taatttttga cgtatcacca cctgccaact
    13981 ttattacctc gtaaaaaact tcagctctgt cacgcgcaaa caaaaggctt tttcctgtta
    14041 agcttgtttg gttctgtaaa tataatcgat taagcgcttc gatcccctta tttctttctc
    14101 catttttaac tctttgatat gaaatcacgc ttacaccttt gtgctttccg gtttcttctg
    14161 cgcctttata ggaatctccg ctttgaaatg aaacctgatg caatcccaaa ttagttatgt
    14221 gcatttaatt atcctcagtc ataaatgggg cattgattgc agttttgtac ctcgttttac
    14281 aacaaaaaag atacttattc aacagggttc tctctttttc tataacactc cgagcctgat
    14341 atatcagaca ctttaacttg tgggatttca tgatgaaacg agcactacat atgctcacga
    14401 ggtacatatt atgtacaaca aaagaaaatt tgattatgtt aacaggctca agtttctaaa
    14461 tttattaatt gaatatatta tatatcaaat acattttaca tccccacaat caccacgtaa
    14521 tggagagtta ataaagtatg atcctcaaaa ttagcaattt cattgacgca agcaatacaa
    14581 aagggcctat acgcgttgaa gatacagagc atggacctat attggtagca cagaaattca
    14641 acctaaagga tctctttttc agaacattaa gcactattaa tgcaaagata aattcacaga
    14701 tattaaatga acaactaaaa aattatcgac tagcgaatca aaaatcctta ttgcttttcc
    14761 tcaagacatt agctagcgaa aaatctgcag aaagtgcgtt tgccgcctat gaagcagtca
    14821 aaaattcaat tcaacattct tttacaggta aagacatcaa attaatgttg aataccgcag
    14881 agcgttttca tggcatcggt acagccaaaa atcttgaaag acatttagtt tttcgttgct
    14941 ggggaaatag aggcataacc catttagggc ataccagtat cagtataaaa aacaatttac
    15001 tacaggaacc aactcatacc tatctcagct ggtatcccgg cgggaatgtt acaaaagata
    15061 cagaaataaa ctaccttttt gaaaaacgct caggttacag tgtcgatacc tataaacaag
    15121 acaaactaaa tatgatttca gaccaaacag ccgaaagact tgatgcaggc caagaggtta
    15181 gaaacttatt aaattcaaaa caagatcaaa ataacaacaa aaaaatattt tttcccagag
    15241 ctaatcaaaa aaaagacccc tatggttatt ggggggtcag tgctgataaa gtatacattc
    15301 cattatcagg tgataataaa acaaaggatg gtaaaatcag ctataacctt tttggccttg
    15361 atgaaacaaa catgtcaaaa tttatatgcc aaaagaaagc agatgctttc agacaattgg
    15421 caaattataa attaatcagt aaatctgaaa actgtgctgg tatggcactt aatgttctta
    15481 aagcaggaaa ttctgaaata tactttccac tccctgacgt taaattggtt gctacaccta
    15541 acaatgtata cgcgtatgca aataaagtta ggcaacgcat tgaaagtctc aatcagtctt
    15601 ataacgagat aatgaagtat attgaatctg attttgatct ttccagattg actcaattac
    15661 gacgtagcta tcttaaaagt ttcaataaaa ttaaccttat ccacacccca aaaacattca
    15721 agcctttatc aatttcactt tacaaacatc ctactgaaaa tgtatcttca gaagactttg
    15781 atgcggtcat caacgcttgc cactcatact tagtaaaatc agcacccagc aatatgtctc
    15841 gtgtattaaa cgaattaaaa accggagcaa cagataaaaa agaagaaatt atcgaaaagt
    15901 caataaaaac tattgattat tacaattctc ttaaatcgcc agatcttgga accaaacttt
    15961 acattcacga cttattacag gtcaataagc ttttattgaa taactctcat tctaatatat
    16021 agaaggccat agaaatgtgt cgcaaactat atgataaact ttatgaaata acaggtgcaa
    16081 agcttgattt taacgataaa aatcaagcct ttatattgct cgaagaacaa atacctgttt
    16141 gtataacaga taatgatgaa tacatctttt tgaccggact tcttaatgaa catgaacttt
    16201 ttacagaaaa tattattaat ccagaacaca tactcatcct taactactct ttgagtagag
    16261 attatggttc ttctatctgt cttctccctg atactcatca atgtgttttg accaagaaac
    16321 actacaaaaa atatctttca cctgatgaac tcattgaatc tttgtatgaa tttttatttt
    16381 gtattaaact aaccattgca aacatcactt cagaagtgaa ctaacatata gggggtatca
    16441 tgcaaattct aaacaaaata cttccacagg ttgaattcgc gattcctcgc ccatcattta
    16501 attcgttaag ttacaataag cttgtaaaga aaatattatc tgtttttaac ttaaaacaac
    16561 gctttcctca gaagaatttt ggatgcccgg tgaatataaa taaaatcagg gacaacgtca
    16621 tagacaaaat aaaagatagt aattcgggga atcaactatt ttgttggatg agccaagagc
    16681 gaacctctta tgtctcttca atgataaaca gaagtataga tgaaatggcc atacacaatg
    16741 gagttgtttt gacctcagat aacaaaaaaa acatatttgc agctattgaa aaaaaattcc
    16801 cggatataaa acttgatgaa aaatcagcac aaacatctat cagtcataca gcactaaacg
    16861 agattgcctc atctggcctc agagcaaaga ttttaaaacg ctatagcagt aacatggatt
    16921 tgtttaacac acaaatgaag gatcttacaa atctagtatc cagttccgtc tacgataaaa
    16981 tatttaatga atcaacaaaa gttttacaaa tagaaataag cgcagaggtt ctaaaggccg
    17041 tataccgtca aagcaataca aattaaatct gtttatatta aatcttatac ttattaaagt
    17101 aaagcagaca aaaaggagac cttatgtctt taaatatcac tgaaaatgaa agcatctcta
    17161 ctgcagtaat tgatgcaatt aactctggcg ctacactgaa agatattaat gcaattcctg
    17221 atgatatgat ggatgacatt tattcatatg cttatgactt ttacaacaaa ggaagaatag
    17281 aggaagctga agttttcttc agatttttat gtatatacga cttttacaat gtagactaca
    17341 ttatgggact cgcagctatt tatcagataa aagaacagtt ccaacaagca gcagaccttt
    17401 atgctgtcgc ttttgcatta ggaaaaaatg actatacacc ggtattccat actggacaat
    17461 gccagcttcg gttgaaagcc cccttgaaag ccaaagagtg cttcgaactc gtaattcaac
    17521 acagcaatga tgaaaaatta aaaataaaag cacaatcata cttggacgca attcaggata
    17581 tcaaggagta attattatgc ataatgtaag taccacaacc actggtttgt ctcttgccaa
    17641 aatattggct tccactgagc ttggagacaa tactatccag gctgcaaatg atgcagctaa
    17701 caaattattt tctcttacaa ttgctgatct tactgctaac aaaaatatta atacaactaa
    17761 ttcacactca acttcaaata tattaatccc tgaacttaaa gcaccaaagt cattaaatgc
    17821 aagttcccaa ctaacgcttt taattggaaa ccttattcaa atactcggtg aaaaatcttt
    17881 aactgcatta acaaataaaa ttactgcttg gaagtctcag caacaggcaa gacagcaaaa
    17941 aaacctagaa ttctccgata aaattaatac tcttctatct gaaactgaag gactaaccag
    18001 agactatgaa aaacaaatta ataaactaaa aaacgcagat tctaaaataa aagacctaga
    18061 aaataaaatt aaccaaattc aaacaagatt atccgaactc gacccagatt caccagaaaa
    18121 gaaaaaatta agccgggaag aaatacaact cactatcaaa aaagacgcag cagttaaaga
    18181 caggacattg attgagcaga aaaccctgtc aattcatagc aaacttacag ataaatcaat
    18241 gcaactcgaa aaagaaatag actctttttc tgcattttca aacacagcat ctgctgaaca
    18301 gctatcaacc cagcagaaat cattaaccgg acttgccagt gttactcaat tgatggcaac
    18361 ctttattcaa ctagttggaa aaaataatga agaatcttta aaaaatgatc tggctctatt
    18421 ccagtctctc caagaatcaa gaaaaactga aatggagaga aaatctgatg agtatgctgc
    18481 tgaagtacgt aaagcagaag aactcaacag agtaatgggt tgtgttggga aaatacttgg
    18541 ggcactttta actatcgtta gtgttgttgc agcagctttt tctggaggag cctctctagc
    18601 actggcagct gttggtttag ctcttatggt tacggatgct atagtacaag cagcgaccgg
    18661 caattccttc atggaacaag ccctgaatcc gatcatgaaa gcagtcattg aacccttaat
    18721 caaactcctt tcagatgcat ttacaaaaat gctcgaaggc ttgggcgtcg actcgaaaaa
    18781 agccaaaatg attggctcta ttctgggggc aatcgcaggc gctcttgtcc tggttgcagc
    18841 agtagttctc gtagccactg ttggtaaaca ggcagcagca aaacttgcag agaatattgg
    18901 caaaataata ggtaaaaccc tcacagacct tataccaaag tttctcaaga atttttcttc
    18961 tcaactggac gatttaatca ctaatgctgt tgccagatta aataaatttc ttggtgcagc
    19021 gggtgatgaa gtaatatcca aacaaattat ttccacccat ttaaaccaag cagttttatt
    19081 aggagaaagt gttaactctg ccacacaagc gggaggaagt gtcgcttctg ctgttttcca
    19141 gaacagcgcg tctacaaatc tagcagacct gacattatcg aaatatcaag ttgaacaact
    19201 gtcaaaatat atcagtgaag caatagaaaa attcggccaa ttgcaggaag taattgcaga
    19261 tctattagcc tcaatgtcca actctcaggc taatagaact gatgttgcaa aagcaatttt
    19321 gcaacaaact actgcttgat acaaataagg agaatgttat ggaaattcaa aacacaaaat
    19381 caacccagat tttatataca gatatatcca caaaacaaac tcaaagttct tccgaaacac
    19441 aaaaatcaca aaattatcag cagattgcag cgcatattcc acttaatgtc ggtaaaaatc
    19501 ccgtattaac aaccacatta aatgatgatc aacttttaaa gttatcagag caggttcagc
    19561 atgattcaga aatcattgct cgccttactg acaaaaagat gaaagatctt tcagagatga
    19621 gtcacaccct tactccagag aacactctgg atatttccag tctttcttct aatgctgttt
    19681 ctttaattat tagtgtagcc gttctacttt ctgctctccg cactgcagaa actaaattgg
    19741 gctctcaatt gtcattgatt gcgttcgatg ctacaaaatc agctgcagag aacattgttc
    19801 ggcaaggcct ggcagcccta tcatcaagca ttactggagc agtcacacaa gtaggtataa
    19861 cgggtatcgg tgccaaaaaa acgcattcag ggattagcga ccaaaaagga gccttaagaa
    19921 agaaccttgc cactgctcaa tctcttgaaa aagagcttgc aggttctaaa ttagggttaa
    19981 ataaacaaat agatacaaat atcacctcac cacaaactaa ctctagcaca aaatttttag
    20041 gtaaaaataa actggcgcca gataatatat ccctgtcaac tgaacataaa acttctctta
    20101 gttctcccga tatttctttg caggataaaa ttgacaccca gagaagaact tacgagctca
    20161 ataccctttc tgcgcagcaa aaacaaaata ttggccgtgc aacaatggac acatcagccg
    20221 ttgctggtaa tatatccaca tcaggagggc gttatgcatc tgctcttgaa gaagaagaac
    20281 aactaatcag tcaggccagc agtaaacaag cagaggaagc atcccaagta tctaaagaag
    20341 catcccaagc gacaaatcaa ttaatacaaa aattattgaa tataattgac agcatcaccc
    20401 aatcaaggaa ttcgacagcc agtcagattg ctggtaacat tcgagcttaa tatatccaag
    20461 agccataata atatatggct cttcctgtaa ggaaataacc atgaatataa caactctgac
    20521 taatagtatt tccacctcat cattcagtcc aaacaatacc aacggttcat caaccgaaac
    20581 agttaattct gatataaaaa caacgaccag ttctcatcct gtaagttccc ttactatgct
    20641 caacgacacc cttcataata tcagaacaac aaatcaggca ttaaagaaag agctttcaca
    20701 aaaaacgttg actaaaacat cgctagaaga aatagcatta cattcatctc agattagcat
    20761 ggatgtaaat aaatccgctc aactattgga tattctttcc aggaacgaat atccaattaa
    20821 taaagacgca agagaattat tacattcagc cccgaaagaa gccgagcttg atggagatca
    20881 aatgatatct catagagaac tgtgggctaa aattgcaaac tccatcaatg atattaatga
    20941 acagtatctg aaagtatatg aacatgccgt tagttcatat actcaaatgt atcaagattt
    21001 tagcgctgtt ctttccagtc ttgccggctg gatctctccc ggaggtaacg acggaaactc
    21061 cgtgaaatta caagtcaact cgcttaaaaa agcattggaa gaactcaaga aaaaatatga
    21121 agataaaccg ctatatccag caactaatac tgttagtcag aaagaagcag ataaatggct
    21181 tacagaatta ggtggaacaa tcggcaaggt atctaaaaaa aacgggggat atgttgtcaa
    21241 tataaacatg accccaatag acaatatgtt aaaaagctta aataatctag gtggaaatgg
    21301 cgaggttgtg ctagataatg caaaatatca ggcatggaat gccggattct ctgccgaaga
    21361 tgaaacaatg aaaaataatc ttcaaacttt agttcaaaaa tacagtaatg ccaatagtat
    21421 ttttgataat ttagtaaagg ttttgagtag tacaataagc tcatgtacag atacagataa
    21481 actttttctc catttctgag gtgcgccatg cataatgtaa ataatactca agcgccaaca
    21541 ttcttatata aggcaacttc accatcatca acagaataca gcgagttaaa aagcaaaata
    21601 tccgatatcc atagttcgca aacttctcta aaaacaccag catcagtgtc tgaaaaagaa
    21661 agctttgcaa cgtcttttaa tcagaaatgt cttgattttt tattttcttc ctcagggaaa
    21721 gaagatgtgt taagaagcat ttattccaac tcaatgaatg cgtatgccaa aagcgagatt
    21781 ctcgaatttt caaatgtttt gtactcctta gtacatcaaa atggtcttaa ttttgaaaac
    21841 gaaaagggac ttcaaaaaat tgtcgcacag tattcggaac taattataaa agataaatta
    21901 tcccaagatt ctgcctttgg accatggtcg gcaaagaata agaaactcca tcaattacga
    21961 caaaacattg agcacagact tgcactatta gcacaacaac acacatctgg tgaagcttta
    22021 tcattgggac aaaaactcct caatactgaa gtatcatcat ttatcaagaa taatattctt
    22081 gctgaattaa agttaagtaa tgaaactgtt tcatctctca aactagatga tttagttgac
    22141 gcacaggcaa aacttgcctt tgatagtttg cgcaatcaac gtaaaaatac tattgatagt
    22201 aaaggatttg gtataggtaa actgtcaaga gacttaaata cagtagccgt gtttcctgag
    22261 ctgttgagaa aagtccttaa tgatatttta gaagatataa aagattcgca tcctatccaa
    22321 gatggcctcc ctacacctcc cgaagatatg ccagatggcg gaccaacccc cggagccaat
    22381 gagaaaacat cccaacctgt aattcactat catataaata atgataatag aacttacgat
    22441 aatagagttt ttgacaacag agtatatgac aatagctatc acgagaaccc agaaaatgat
    22501 gcacagtctc ctacttctca gacaaacgat ctattatccc gtaacggaaa ctcattacta
    22561 aatccacaaa gagcactagt tcaaaaagta acttccgttc taccacactc tatatcagat
    22621 actgtccaga catttgcaaa taattcagct ttagaaaagg ttttcaacca tactccagat
    22681 aattcggatg gaataggttc agacctgtta actacgagta gtcaagaaag atctgcaaat
    22741 aactctcttt ctcggggaca caggcctctg aacatacaga actcttcaac caccccccct
    22801 ctccacccgg aaggagtgac aagcagtaat gataactcat cagatacaac taaaagtagc
    22861 gcttctcttt ctcatagagt agcttcgcaa atcaataaat tcaactcaaa cactgattca
    22921 aaagtacttc agactgattt tttctcaaga aatggagaca catatttaac acgggaaacg
    22981 atatttgaag cgtcaaaaaa agtaacaaac tccctaagta atcttatatc tctcattgga
    23041 actaaatcag gaacacaaga acgagagtta caggaaaaat caaaggacat tacaaaatcc
    23101 acaacagaac acagaataaa caacaaatta aaagttacag atgcaaatac aataaactac
    23161 gtaacagaaa ccaacgcaga tacaattgat aaaaatcatg cgatctatga aaaggcaaaa
    23221 gaagtatcta gcgccctcag caaggtattg tcaaaaattg acgatacctc tgcagaatta
    23281 cttacagatg atatatctga tttaaaaaat aacaatgata ttacagctga aaacaataat
    23341 atatataaag cagcaaaaga tgtaaccact tccctatcaa aagtattaaa gaatatcaat
    23401 aaggattaaa atatgataaa agaaaaaata ttatcaatag tggccttttg ctatggtata
    23461 gcgtacagca aactatctga agaaactaaa tttattgaag atctttctgc cgattctctt
    23521 tctctgattg aaatgctgga tatgattagt tttgagttca atctacgtat agatgaatct
    23581 gcattagaac acattattac tattggagat cttatttctg tagtcaaaaa tagtacaaaa
    23641 tcaatatagt aattgataag cattttttca tctatggagc tctcacatca gagctccaca
    23701 agaatattat tcttttatcc aataaagata aattgcatca atccagctat taaaatagta
    23761 atttttaaga ctaccgttga ctatcatcaa cagttacata caatcttgct cacactgcat
    23821 ttaacttttg tcaatataaa tcaatatgaa taaacagggt gtgatatggt ggatttgtgc
    23881 aacgacttgt taagtataaa ggaaggccaa aagaaagagt ttacactcca ttctggtaat
    23941 aaagtttcct ttatcaaagc caagattcct cataaaagga tccaagattt aaccttcgtc
    24001 aaccaaaaaa cgaatgtacg cgatcaagaa tccctaacag aagaatcatt agccgatatc
    24061 ataaaaacta taaagctaca acaattcttc cctgtaatag gaagggagat tgatggtaga
    24121 attgaaattc tggatggcac tcgtagaaga gcatctgcaa tatatgcagg agcagatctt
    24181 gaagttctat attcaaaaga atatatatct actcttgatg ccagaaaact agcaaacgat
    24241 atacaaacag caaaagagca tagcatccga gaacttggta tcggtcttaa ttttctgaaa
    24301 gtatcaggga tgtcctataa agacatagcc aaaaaagaga atctgtctcg cgcgaaagtc
    24361 actcgtgcct ttcaggcagc aagcgttcca caggaaataa tatctctatt tccaatcgcg
    24421 tcagaactta actttaatga ctacaagata ttattcaatt attataaagg acttgaaaag
    24481 gctaatgaat ctcttagttc tacactacca atattaaagg aagaaataaa agaccttgat
    24541 acaaatttgc ccccggacat atataaaaaa gaaattttaa acatcataaa gaaaagcaaa
    24601 aacagaaagc aaaacccttc gctaaaagtt gactcgttat ttatttctaa agacaaacgg
    24661 acttacataa aaagaaaaga gaataaaaca aacagaactc taatatttac attatctaaa
    24721 ataaacaaaa cagttcagag agaaatagat gaagctattc gggatataat ttctcgccat
    24781 ctatcgtctt cataaacatc gaacactgat gtaaactgcc caattcagat aataaaaatc
    24841 ttcgttgaac ttgactttca gccggtgggg taattccgtc ggctatctgt tattcaatcc
    24901 ccacgcactg tgtttcctta aaattacggt gaaaactcat accctgctga ccacgagaac
    24961 attatctggt gaaattagta atattcatat cattgaaaat gaattgtcaa ctaagggatg
    25021 cggcatgatg gtcaaaattt tatgtgaaga tgagtgactg gccgaaaata ttcagcgtat
    25081 ttttaattgt ttcaataaaa acagcgagga ttagcagtca acgcttttac tactcaattt
    25141 ttttacatgg tgataaacaa aaaatccacc attaacagac ttcgttcaca gtatcaaaaa
    25201 agctttaaga atatattagc agatttctca aatataatta ctgttttatt ctcctaatct
    25261 tcccataaaa tgaccgaatt acattatttt ataattataa acaagtttga ttttaaaatc
    25321 atattaatta ttaacaagag atttattttt ctaaccccaa aagttatgct tagtcaaaga
    25381 gtaacttata atatccatac ccctcatagg gcttacagtt taaattgcgg gtataaaata
    25441 catctatttt caaataaaat aaagcatact ccaattacaa cgagtgacta aaataaaaat
    25501 atcatccaag tcaatcactg tttgctctgc aaaatatgtc agccttctca aacttaccag
    25561 cacttaaata tttaagcaca caatcattcc ttgagggagt tcacttattt caagagaaac
    25621 atttcgtctt cccacacaac taatccgccc aatatagcta aatttgtact cagtaatatc
    25681 atttgttatg ccccccgccc ccaaaatatt caagctcctt cacagataaa aaacgacaat
    25741 ttacaaagcc tcattagtta taactatgga aatacctatt ttagtatatc ctataaagtt
    25801 agaattactt cttcttgcac ctaattccat agttgaaaaa caagaataat tttctcctac
    25861 agctggatcc atatagcttc catcaggtct acacaacacc cagtggaggc cgacaggaac
    25921 tccaataaag gatacagcaa cagcttccaa aactctttgg ttagaagata acacatctcg
    25981 ttgatgtact atattacatc ccatgccaat taataagtcc cttgcatcgg gataaataaa
    26041 actaattact tgcgaaaaaa tgttactttc ttcaacgaca taagcattca atccaagaag
    26101 cctcgtagcc atgactatct tatcaggcat agaataaccg gcatccgcga tattatcttt
    26161 gtgtattctc ggattataat taccactagt tattaagtat aaatcccgct cacaacgatt
    26221 atccaaatca agagaactct tccttgtcat ttcggacata gaccctttgt attgtggtat
    26281 tttatcaacc cctaattcct ttgcagcaca gagtaaactg gcagctccac aagaattgtc
    26341 atacatctgc ttcctcgcca aaaaacaacc ttgttcgttt actctattct catttaccct
    26401 cacatcagtc tgatgagata tcatatattc tcttggtata gccccctgca ataaaacccc
    26461 atataacata acacctgtat gaatacatcc ccgtttgcaa ggcttccgtt gttccgacat
    26521 cgacgagtaa ttgcagcaca gtattgcatc cagcaatgcg aactactatg agaattagct
    26581 gaccgggtcc ttattccccg aacgaacttt aaatccaaaa cctcatactc ttttccgtcg
    26641 tcatcacgtc cgtcaccata acaacctttc agcatccacc cttttattaa gagttaatgt
    26701 ccatcagcat cagcaattaa taccgcctga tactgacata tttttgtcgt atttacgtcc
    26761 ataatcggtt attgtgattc aggtcacacc agcttatttg ctattgttac aataaccacc
    26821 ttggttgaga gctgtttttt caactttgtt atcgactccc ccaactcctt ggtcagtaag
    26881 gctgccgacc gagcacagtg aatagatagt atccgcaggt accaccatgc ttacttaata
    26941 ctcagaaggc gaagtgctct ccaattaact ctgccgcact atgcttataa gctgcttttc
    27001 aatgccgtct atcaacatgt ccagttcatt caatctgttg tactggtaat ccaacgcagt
    27061 aatgatatat gcaggtattc tttcatccac tccagtaacg tttcatgcaa agcattaatt
    27121 tacgcgctac ggaacttcac cagttgactg agcaaacgat gtgggaccag tactgcctgc
    27181 agttcttcta ttgttacagc gatttcttta ccaaacagct gaaccaccat caagataaag
    27241 atagcctgaa cattcatcgc accattttgt taccaatggc aaaagattta acgaaccgtg
    27301 cctgcatcag gcggactttt tatccctgct ttgttagctc cctaaccttg tacagagagc
    27361 ctccataagc tcccatatca agcagacatg gttcgcagtt actgacatac accagaaaac
    27421 tctgacagca gactcactta tcctccactt cactcatatc cttattggtg agatgaacct
    27481 gaatgacatg ttttgcgaca taaacgccaa ccagtgtata tttcacctgt agagattcta
    27541 caatctgtag cgttatgcat ctaatgttag gctctatgat gtcagaaatc tgcgagactc
    27601 tacaccttct gcatttacct ttatttatca ccatgctcct cagttaacgg agaacgtgtt
    27661 cattgcattt tgtcacttac atttcgcctt tggtaccggg gggatatagg ttccaacgtt
    27721 ccgcatgaga acctgaaccg gcttcacgcc accttgatac cagatgccgc acaggcagtc
    27781 aacaa
//
LOCUS       DAJTYZ010000047        26979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000047 DAJTYZ010000000
VERSION     DAJTYZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26979
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.047"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1334)
                     /gene="rsmF"
                     /locus_tag="OO596_001906"
     CDS             complement(<1..1334)
                     /gene="rsmF"
                     /locus_tag="OO596_001906"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6717771.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVT"
     gene            complement(1415..4048)
                     /gene="yebT"
                     /locus_tag="OO596_001907"
     CDS             complement(1415..4048)
                     /gene="yebT"
                     /locus_tag="OO596_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR6717772.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4017..5300)
                     /gene="yebS"
                     /locus_tag="OO596_001908"
     CDS             complement(4017..5300)
                     /gene="yebS"
                     /locus_tag="OO596_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6717773.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5430..5927
                     /gene="msrC"
                     /locus_tag="OO596_001909"
     CDS             5430..5927
                     /gene="msrC"
                     /locus_tag="OO596_001909"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6717774.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6024..6722
                     /gene="proQ"
                     /locus_tag="OO596_001910"
     CDS             6024..6722
                     /gene="proQ"
                     /locus_tag="OO596_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6717775.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            6742..8790
                     /gene="prc"
                     /locus_tag="OO596_001911"
     CDS             6742..8790
                     /gene="prc"
                     /locus_tag="OO596_001911"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6717776.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            8982..9863
                     /gene="htpX"
                     /locus_tag="OO596_001912"
     CDS             8982..9863
                     /gene="htpX"
                     /locus_tag="OO596_001912"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6717777.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(9909..10972)
                     /locus_tag="OO596_001913"
                     /pseudo
     CDS             complement(9909..10972)
                     /locus_tag="OO596_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11149..11940
                     /gene="kdgR"
                     /locus_tag="OO596_001914"
     CDS             11149..11940
                     /gene="kdgR"
                     /locus_tag="OO596_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6717778.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12083..12322)
                     /locus_tag="OO596_001915"
     CDS             complement(12083..12322)
                     /locus_tag="OO596_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717779.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12481..12624
                     /gene="mgrB"
                     /locus_tag="OO596_001916"
     CDS             12481..12624
                     /gene="mgrB"
                     /locus_tag="OO596_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6717780.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            12699..12986
                     /locus_tag="OO596_001917"
     CDS             12699..12986
                     /locus_tag="OO596_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6717781.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13656..13799
                     /locus_tag="OO596_001918"
     CDS             13656..13799
                     /locus_tag="OO596_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6717782.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            13812..14021
                     /gene="cspE"
                     /locus_tag="OO596_001919"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             13812..14021
                     /gene="cspE"
                     /locus_tag="OO596_001919"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6717783.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14187..14996
                     /gene="rlmA"
                     /locus_tag="OO596_001920"
     CDS             14187..14996
                     /gene="rlmA"
                     /locus_tag="OO596_001920"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6717784.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(14993..15559)
                     /gene="mntP"
                     /locus_tag="OO596_001921"
     CDS             complement(14993..15559)
                     /gene="mntP"
                     /locus_tag="OO596_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6717785.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(15988..16446)
                     /locus_tag="OO596_001922"
     CDS             complement(15988..16446)
                     /locus_tag="OO596_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6717786.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16501..17352)
                     /gene="manZ"
                     /locus_tag="OO596_001923"
     CDS             complement(16501..17352)
                     /gene="manZ"
                     /locus_tag="OO596_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6717787.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17365..18165)
                     /gene="manY"
                     /locus_tag="OO596_001924"
     CDS             complement(17365..18165)
                     /gene="manY"
                     /locus_tag="OO596_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6717788.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18228..19199)
                     /gene="manX"
                     /locus_tag="OO596_001925"
     CDS             complement(18228..19199)
                     /gene="manX"
                     /locus_tag="OO596_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6717789.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19662..21212
                     /gene="yoaE"
                     /locus_tag="OO596_001926"
     CDS             19662..21212
                     /gene="yoaE"
                     /locus_tag="OO596_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6717790.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(21216..22814)
                     /locus_tag="OO596_001927"
     CDS             complement(21216..22814)
                     /locus_tag="OO596_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6717791.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(22945..24309)
                     /gene="sdaA"
                     /locus_tag="OO596_001928"
     CDS             complement(22945..24309)
                     /gene="sdaA"
                     /locus_tag="OO596_001928"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6717792.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24493..25071)
                     /locus_tag="OO596_001929"
     CDS             complement(24493..25071)
                     /locus_tag="OO596_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6717793.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25075..26436)
                     /gene="pabB"
                     /locus_tag="OO596_001930"
     CDS             complement(25075..26436)
                     /gene="pabB"
                     /locus_tag="OO596_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6717794.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26510..26689
                     /locus_tag="OO596_001931"
     CDS             26510..26689
                     /locus_tag="OO596_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6717795.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(26809..>26979)
                     /locus_tag="OO596_001932"
     CDS             complement(26809..>26979)
                     /locus_tag="OO596_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6717796.1"
                     /translation="ASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYF
                     STYMQEELKALV"
BASE COUNT         6954 a         6645 c         6836 g         6544 t
ORIGIN      
        1 aaagtaacca acacgtcatc cgccactggc gcggcttgcg ggtaaacatc gcgcccgcga
       61 taccactcct ccgcttcctg cggtgtcagt tcaaaagcgt tcacattgtc gggggtggca
      121 agggcaataa ctgcttcatg ctgccagcga taacctttgt tgtgcgtttc ggcaagttta
      181 atccccaagc gagaaaatcg gactttaccg atcagggctt caatgcccac cgggaacaac
      241 cacagttctt tgtcacgctg ccagaggcgc aggttttcat cccagtttaa gccaacacct
      301 gcagccgcct gacgaatttg tccagcttct cgatctttca ctgggctgaa cggaaaatta
      361 ccgactttgt atttgggggc gggtaaggcg ggaatcgcct gagttttacg cagacgagca
      421 acgaagaagc cttcgcagtc gtaaatttgt gggaaaacat gcaaaaagcc ttcctcggtc
      481 agcgccttgt ttgcaccagg gaagagatca ccaagcggta aaaactctac tgcgtcgggg
      541 taagtctctt tcagccacag gcaaacggct tcgttttctt cctggtttaa ggtacaggtc
      601 gagtaaacca gcgtaccgcc agggcgtaat gcatgaaagg cgctgtcgat cagttcccgt
      661 tgggtagctg cgatttcctg attgctttct ggtgaccagt tttttagcgc atcgggatct
      721 ttacgcacca cgccttcgcc ggagcaggga gcgtccagca aaatggcatc aaacatttct
      781 ggcactgccg caccaaacac gcggccatca aaatgtgtga gcgcaacatt actgatacca
      841 cagcggctga tattggcatg taacactttt acccgactgg cggaaaactc attggcaaga
      901 attgcccctt cgttattcat ccgcgcggaa atttgcgtcg ttttggagcc aggcgcggca
      961 gcgacatcca tcacccgctg tggtgcatta ccgtcagcaa acaaggcggc gacgggcaac
     1021 attgaactgg cttcctgaat ataaaacagg ccacttaaat gctcggcggt actacccaat
     1081 ggcaatgcat cttcattgtc gcgttcaatc cagaaacctt cttcacacca cggaattggc
     1141 gtaagcgtcc agccataagg agcggttaat tgcaggaaat cagcaacgga gattttcagc
     1201 gtattaacgc gaatgctgcg gcgcaacggg cgctgacagg cggcaagaaa atcatcaaaa
     1261 gagagcgtcg aaggcatcgc ttcgcgcatt tgcgtcagaa aggcgtccgg gaaataaacg
     1321 gtgtgttggg ccacgagcat ataccactgg caaaaaaaac aggcgcgcag tttagcataa
     1381 acgctccggc gcaggcacgc cggagcagtg ggcattattt gggaagcgca gtaccccatt
     1441 cacgccactc tttcggttca ctttcctgca acaggaagtg cttgccttcc tgggctttcg
     1501 gtgccagtgg cgtacccgga ggcgtggcga aggcgatgcc gccacggata aactggttaa
     1561 aggtgccggt tttcactacg ccgcccgtca gaccaaagtc cagactgtaa cccgatgcca
     1621 accagaagac ggaattgtta cgcaccaggt gttgatagcg tttactgatg cgcatcgcaa
     1681 tcatcacgcg atctgacaat gtccccagcg tcattccggt aaccgtacct acttccagac
     1741 cacggaacag cacaggcgta ccaataccta acgaaccggc ttccggtgct tcgacaataa
     1801 tgcttaagcc atccaggtaa cgcgaatcag taatagtagc ttcttgcaac tcaaagtcac
     1861 ggcgaggatt accccgacct ggttcgacat tgatatacgg ctggaggata gtatctagat
     1921 gctcaacgcc cgcggctgaa atttgcggtg tgaccaccga gaagcgcgta ccgccgcggg
     1981 cgaaggtctg gacatactcc ggatagagca ccgcctttgc ctgtacttca ttgcgcgcgg
     2041 taatcagatc cagcgtctgg atttgcccga tatcaatacc gagatagcga attggcatcc
     2101 cgaccgccag ttttccggca tcgaaagcgt gaagcgtaat ctgcccacca accgcacggg
     2161 ccgctgtttc ggaagcatac agaattcgtt tgtcgccttt acgctgactg gcgctggcac
     2221 cgctgaggtt atcgaagcta atggctccct ttaatgctct ggagagcggg gatgcctgta
     2281 cggtcaggcc actaccattc agctgaactt tcgccccgcc ttctgcccag aacacgctat
     2341 tgctggtcag aaggttgcga tactccggct taatatgcag atcgatatca aacgcgttag
     2401 ctcgcggacg cacggtaatc acttcaccaa cttcaaattt acggtacagc actaccgatc
     2461 ctgcctgcac atccggcagc gtctctgcac tcaaactcac ggttgtggtg ggtaaatcgc
     2521 taaggctgtt ctccagcgct ttttccagat tggcatacag tggatagctg gctttcatct
     2581 cacctttatc gcctggcaga atacgtatcc cgccgttaat ccattctgag gcgctggcac
     2641 caagaaactc aacgccatcc agccccacct tcacgtcgac acggctgttg acgacaaatt
     2701 tgctatcgcc ttttaccagt tcgcgatgct gaggctcgat ggcgacggta aaggtaacgc
     2761 ctttgctggt gagtttacga tcaataacct ggcctacctg cacgccgtga agaatgagcg
     2821 gctgaccagc atcaataccg taactttccg gcgcggtcag ggtcagcgtc agaacatcag
     2881 gttcatgcag cagtgctttt tcgcctggca caacaacgaa ctctttgcgt ggctcgccat
     2941 cgccgggtac caactcgaag gttttgccag tcagcagggc gctgagattg gcatcgctaa
     3001 gggataattt cggattaagt aattcgatgc gggtattttc acgaagcagg gtaacgacgc
     3061 tgggattaac tgtcatttcc ccagtgactt taccaccagg atttaaatcc agtttagtta
     3121 gctgtccgac ttccagtccc tgatacatta atggcgtcga gtcggcggtt aatccggccc
     3181 cactcggcag ttccagtttg attattacgc cacgctggct atgggcgagg tcttcataca
     3241 gaccaaaggt atcttccgcc tcggcaggtt tcgactcttc aggtgaatcg aaggcaatcg
     3301 caccgttaac cagtgccgcc agactttcca gtttcacctt cgcaccactg atactgacgt
     3361 tggcatcaac gccggaaacg ttccagaaac ggctaccttt tttcactagg tcggtaaaac
     3421 gccgctcgat caggacatca atcaccacgc cttgcttgtt gggattgatg gcatagtcgt
     3481 agacttttcc cacagggatc ttgcggaaat agaccaatga accgctgttc agcgaaccga
     3541 gatcgggggc ttgcaggtgg atcatcagat cgccattgtc cagtcgatat ttcggttggg
     3601 tatcgagtgc gacaaagtga tcctgctctt tacctttacc tggcatcatg ccgatatagt
     3661 taccaccgac gagggcgtcc agcccggaga cacctgccaa cgatgctttt ggcgtcacca
     3721 gccagaactg agtctcttcg cgcagcgcat ctttcatatc ggacttgatg ctgaccttga
     3781 cttcaatctt acgaagatcg tcgctgaggc tgatatcctg cactgttccg acttcaacgc
     3841 cctgataacg aacaggcgta cggcccggga caataccatc cgccgacata aagtcgatgg
     3901 tgacggtatt accccggtcc tgataactgt cccaaatcag ccaaccggca atcattagcg
     3961 cgatgaaagg cagcagccag aaaggtgaga tacggcgttt atttttaatc tgcgcttcag
     4021 tcgtcgaagc gggcgtttcc tgactcatgt gcatcccaaa gtaagcggct gtccagccat
     4081 tccacagcaa gaatagtcaa aattacggct gcgccaaaat aaaacgcagc cggtcccata
     4141 gtaaaagcga ggatctgatc gcgattaatc agcgacatgg ttaaagatat gacaaacagg
     4201 tcgagcatcg accagcgtcc aatccaggtc accatccgca gtaacagaat gcgtgtgcgt
     4261 aaaccttgct ggcatttaaa atgaatgctc aacagtaaag tgaacatgac gatcacttta
     4321 gtaaacggta ccagaatact ggcgataaac acgattccgg cgaccgcaat gttgctactt
     4381 gccagcgaca taatcccgga aagaatcgta tcttcctgcc ggccaccatt cagataaatg
     4441 atagaaatag gcaataggtt ggctggtaac aacaaaacga ttgacgctaa cagcgccgcc
     4501 cagcattttt gcaaactatg acgacgacgc aggcgtagcg ggatatggca acgcgggcag
     4561 cgaccacgct gatcaggata gccggtaaaa tggcacccaa gacagacacg aagtttctcg
     4621 tccctacgcg tagcggggcg ctgcggataa tatcgctccc acagctcctc aacattaaga
     4681 tgtgacaacg tcaccgtcgt taaaatcacc aacgccacaa aagagaacaa gccgacaccc
     4741 gcctggatat gggcataatc ctgtaccttt atagaagcaa cgccaatgcc gaccaggtag
     4801 atatccagca ttacccactc tttaagacgc tcaagcatca gcagtaccgg acgtaaattc
     4861 attcccagtc ggttaccaaa ccataaatat gctatggagg acaccagaat gaggggggca
     4921 ccaataacgc agaaaaagac catcgccccc gttatcgtat cgccctgttt ggtcatttgc
     4981 cagatgcctt gcatgacgtt ggcgtcgata cgaataccta acagccagat atgcaacagc
     5041 ggttcgcccc aggcaaacgg catcagcaac agcatggtga aagccattgc cgccaggcgc
     5101 gttagcgacc agtcgcgccc gtcgcgaatt tttgcctgac agcgcggaca ataggcactc
     5161 tgatgagaat ttatctcggg caggctaaac agcatgtcac attgcgggca acgttggtaa
     5221 tcaccacgcg gcagttcctc gccgattgcc ctcactgtta tcttttttgt cggcgtaatt
     5281 tgtggtgtgt taagagccat gacgtgccaa atgtaataag aattaacatt aatcttaacc
     5341 catgatggga atgatcttga gcattaacgc atttaatgct tattttaata ggctggcagt
     5401 tccaggtaag acaaacaagt gattatataa tgaacaaaac agaattttac gcggatttaa
     5461 atcgcgactt taacgcgctg atggcgggag aaaccagttt tctggcaacg cttgcgaaca
     5521 ccagtgcgtt gttatacgag cgtctcactg acgtaaactg ggcaggtttt tatttgcttg
     5581 aggacgatac actggtactc ggaccatttc agggcaaaat tgcctgtgtc cggatacccg
     5641 tcgggcgcgg ggtgtgcggc actgcggttg cccgcaatca agtgcagcgt atcgaggatg
     5701 ttcacgcgtt tgacgggcat attgcctgtg atgcggcgag taattctgaa attgttctgc
     5761 cgctggtggt gaaaaatcag attattggtg ttcttgacat cgatagtacc gtcttcggtc
     5821 gctttacaga cgaggacgag caaggcttac gtcagcttgt ggcacagctt gaaaaagtgc
     5881 ttgcaacgac ggattacaaa aaattctttg cgagcgtcgc aggataatca acggataacg
     5941 tagcatttac tggtagcgtc attataatga cgcctgttca tgcctgcgct tgttggctac
     6001 gtccgttgta atcaggaaat ttcatggaaa atcaacctaa gttgaatagc agtaaagaag
     6061 taatcgcgtt tctggccgaa cgttttcccc actgtttcag tgcggaaggt gaagcgcgtc
     6121 cgctgaaaat cggtattttt caggatttgg tcgatcgtgt tgccggggaa atgaacctga
     6181 gcaaaacgca attacgatcc gctttacgtc tctacacttc gagctggcgt tatctttacg
     6241 gtgttaaacc cggcgcaacg cgtgtcgatc ttgacggcaa cccatgcggt gagctggacg
     6301 agcaacatgt agagcatgct cgcaagcagc ttgaagaagc gaaagcgcgt gttcaggcac
     6361 agcgtgctga acagcaagcg aaaaaacgcg aagctgccgc agctgctggt gagaaagaag
     6421 acgcactgcg ccgcgaacgc aagccacgtc cgaccacgcc acgccgcaaa gaaggcgctg
     6481 aacgtaaacc tcgtgcgcaa aagccggtag agaaagcgcc aaaaacagta aaagcacctc
     6541 gcgaagaaca gcacaccccg gtttctgaca tttcagctct gactgtcgga caagccctga
     6601 aggtgaaagc gggtcaaaac gcgatggatg ccaccgtatt agaaatcacc aaagatggcg
     6661 tccgcgtcca gctgaattcg ggtatgtctt tgattgtgcg cgcagaacac ctggtgttct
     6721 gaaacggagg ccgggccagg catgaacatg ttttttaggc ttaccgcgtt agctggcctg
     6781 cttgcaatag caggccagac cttcgctgta gaagatatca cgcgtgctga tcaaattccg
     6841 gtattaaagg aagagacgca gcatgcgacg gtaagtgagc gcgtaacgtc gcgcttcacc
     6901 cgttctcatt atcgccagtt cgacctcgat caggcatttt cggccaaaat ctttgaccgc
     6961 tacctgaatc tgctcgatta cagccacaac gtgctgctgg caagcgatgt tgaacagttc
     7021 gcgaaaaaga aaaccgagtt aggcgatgaa ctgcgttcag gcaaactcga cgttttctac
     7081 gatctctaca atctggcgca aaagcgccgt tttgagcgtt accagtacgc tttgtcggta
     7141 ctggaaaagc cgatggattt caccggcaac gacacttata accttgaccg cagcaaagcg
     7201 ccctggccga aaaacgaggc tgagttgaac gcgctgtggg acagtaaagt caaattcgac
     7261 gagttaagcc tgaagctggc aggaaaaacg gataaagaaa ttcgtgaaac cctgacacgc
     7321 cgctacaaat ttgccattcg tcgtctggcg caaaccaaca gcgaagatgt tttctcgctg
     7381 gcaatgacgg cgtttgcgcg tgaaatcgac ccgcatacca actatctttc cccgcgtaat
     7441 accgaacagt tcaacactga aatgagtttg tcgctggaag gtattggcgc agtgctgcaa
     7501 atggatgatg actacaccgt tatcaattcg atggtggcag gtggtccggc agcgaagagt
     7561 aaagctatca gcgttggtga caaaattgtc ggtgttggtc aaacaggcaa gccgatggtt
     7621 gacgtgattg gctggcgtct tgatgatgtg gttgccttaa ttaaagggcc gaagggcagt
     7681 aaagttcgtc tggaaatttt acctgctggt aaagggacca agacccgcac tgtaacattg
     7741 acccgtgaac gtattcgtct cgaagaccgc gcggttaaaa tgtcggtgaa gaccgtcggt
     7801 aaagagaaag tcggcgtgct ggatattccg ggcttctatg tgggtttgac agacgatgtc
     7861 aaagttcaac tgcagaaact ggaaaaacag aatgtcagca gtgtgatcat cgacctgcgt
     7921 agcaatggcg gtggggcgtt gaccgaagcg gtatcgctct caggtctgtt tattcctgcg
     7981 ggtcccattg ttcaggtccg cgataacaac ggcaaggttc gtgaagacag cgataccgac
     8041 gggcaggtgt tctataaagg cccgctggtg gtactggttg accgtttcag tgcttcggct
     8101 tcagaaatct ttgccgcggc aatgcaggat tacggtcgtg cgctggttgt gggtgaaccg
     8161 acgtttggta aaggcaccgt tcagcaatac cgttcattga accgtattta cgatcagatg
     8221 ttacgtcctg aatggccagc gctgggttct gtgcagtaca cgatccagaa attctatcgc
     8281 gttaacggcg gcagtacgca acgtaaaggc gtaacgccag acatcatcat gccgacgggt
     8341 aatgaagaaa cggaaacggg tgagaaattc gaagataacg cgctgccgtg ggatagcatt
     8401 gatgccgcga cttatgtgaa atcaggcgat ttaacggcct ttgggccgga gctgctgaag
     8461 gaacataatg cgcgtatcgc gaaagatcct gagttccaga acatcatgaa ggatatcgcg
     8521 cgcttcaacg ctatgaggga caagcgcaat atcgtttctc tgaactacgc tgtgcgtgag
     8581 aaagagaata atgaagatga tgcgacgcgt ctggcgcgtt tgaacgaacg ctttaaacgc
     8641 gaaggtaaac cggagttgaa gaagctggat gatctaccga aagattacca ggagccggat
     8701 ccttatctgg atgagacggt gaatatcgca ctcgatctgg cgaagcttga aaaagccaga
     8761 cccgcggaac aacccgctcc cgtcaagtaa tatcaatcag gcacaagaaa ttgtgcctga
     8821 ttttttaaca gcggcaagat gccgtaaatc agatgctaca aaatgtaaag ttgtgtcttt
     8881 ctggtgactt acgcactatc cagacttgaa aatagtcgcg taacccatac gatgtgggta
     8941 tcgcatattg cgttttgtta aactgaggta aaaagaaaat tatgatgcga atcgcgctct
     9001 tcctgctaac gaacctggcc gtaatggtcg ttttcgggct ggtactgagc ctgacaggga
     9061 tacagtcgag cagcgttcag gggctgatga tcatggcctt gctgttcggt tttggtggtt
     9121 ccttcgtttc gcttctgatg tccaaatgga tggcattacg atctgttggc ggggaagtga
     9181 tcgagcaacc gcgtaacgaa agggaacgtt ggctggtcaa tactgtagca acccaggctc
     9241 gtcaggcagg gatcgctatg ccgcaagtgg ctatctacca tgcgccggac atcaacgctt
     9301 ttgcaaccgg tgcgcgccgt gatgcctctc tggttgctgt cagcaccggt ttgctgcaga
     9361 acatgagccc ggatgaagcc gaggcggtaa ttgctcacga aatcagccac atcgccaatg
     9421 gtgatatggt caccatgacg ctgattcagg gcgtggtgaa caccttcgtt atctttattt
     9481 cccgtattct ggcgcagctt gccgcgggtt ttatgggcgg aaatcgtgat gaaggtgaag
     9541 agagcaacgg caacccgctg atctactttg cggttgcaac ggttctggaa ctggtgtttg
     9601 gtattctggc gagcattatc accatgtggt tctcgcgtca tcgtgaattc catgctgatg
     9661 ccggttcggc aaaactggtt ggtcgcgaga aaatgattgc cgcgctgcag cgcctgaaaa
     9721 ccagctatga accgcaagaa gcaaccagca tgatggcttt ctgcattaac ggtaagtcga
     9781 aatcgctcag tgagttgttc atgactcacc cgccgctgga taaacgaatt gaagctctgc
     9841 gtacgggtga atacctgaag taatatgaag taaatactaa aaagcgcgtc ctgatcgacg
     9901 cgcttttttt atgccttgga tcgtggctga gtgatacgta aaccactgac acaggcagca
     9961 atcactgcca gaatagccgc agccatcagc gaaacgtgcg taccattatc accgaactga
    10021 tttagcatca gcgccaccag tgccgcgccg ctactctgac ccagtagacg agccgttcct
    10081 aacatgccac tggctccacc gctacgttcg cgaggagcgg aggtaataat ggtgtggtta
    10141 ttgggtgact ggaacaagcc aaatccagcg ccacataaga tcatcggcca gataatattg
    10201 atatccgcag gtgacgcggg cagcagaacc aggggaaaaa gccccgcagc catgatgaac
    10261 aaccctagag cacccagcaa tcctgcatgt acgcgttcaa tcaaatagcc tgccaacgga
    10321 gccatcacca tcgttgctaa cggccacggt gtcagaagta aacctgtttc gacttcacta
    10381 cgcccgagca cggtttgcag gtaaaagggc agggaaacca ttgccagcat ttgtgcgcag
    10441 aaagagcaaa cagatgtgca aatagaaagt gaaaacagcg ggatacgcag taaatccacc
    10501 ggtagcagcg gtacgggaag agaaagctgg cggcgaataa agaaaatacc aacaacaacc
    10561 attaccacca gttccgcagc aattaacgtc agcgattgcc cctgagcgaa accactcaac
    10621 gcagtgataa gcaggccgaa ggttaacgcg ttcatcacgg cgctgggcag gtcgaaacgg
    10681 ggtttactgg cgcgagaacc attgggtggc agaaaacgca tcgccagaag cagggcgata
    10741 atacctaacg gtacgttgat taaaaataac catttccagg atgcgatgga gaggattgct
    10801 gcagcaattg tcggcccggc agcagaagag acggcaacaa tatggcgatt gttggcaggg
    10861 cgacgttggc gattgcgccg tcaaggacag ccatcgaaat gccaatcaca atggttaata
    10921 tcgcaccgta tcgctggggc aatggcaggc cgtcggcctg aacttttggc ataggaattt
    10981 tatatctttg gtgaataatc aacaggggta tgattctagc actgatattc acggtggatg
    11041 tcgcagaatt gtaactaacc ggcaaaagat ggattgcgac caccctggtg cgaaattata
    11101 ataaaaaaca gttctgattt ttataaaaca ctcgcaatga ggtgataaat ggctaacgca
    11161 gatctggata aacagcctga ttctgtatct tccgtgctaa aagtttttgg cattttgcag
    11221 gcgctgggtg aagagcgcga aatagggata accgagctgt cgcagcgcgt catgatgtca
    11281 aaaagcaccg tttatcgctt tttacagacc atgaaaacct taggttatgt ggcgcaggaa
    11341 ggggagtcgg agaaatattc cctgaccctg aaattgtttg aactgggcgc tcgcgcgtta
    11401 caaaacgtcg atttaattcg tagcgcagat atccagatgc gtgagatctc ccgcctgacc
    11461 aaagaaacta tccacctcgg cgcactggac gaagacagta ttgtttacat tcacaaaatt
    11521 gactctatgt acaatttgcg catgtattca cggattgggc gtcgtaatcc gctgtacagc
    11581 accgcgattg gtaaggtact gctggcatgg cgcgatctcg atgaagtgaa gcaaattctt
    11641 gagggcgtgg agtataaacg cagtaccgag cggaccatca ccagtacaga agcgttatta
    11701 cccgttctgg accaggtgcg cgagcagggg tatggcgaag ataacgaaga gcaggaagaa
    11761 gggctgcgat gcattgcggt accggtattt gatcgctttg gcgtggtcat tgccggtttg
    11821 agcatctcct tcccgacgtt gcgtttctct gaagagcgtt tacaggaata tgtcgcaatg
    11881 ttgcataccg cagcgcgcaa aatttctgcc caaatgggtt atcacgacta tccgttctga
    11941 tgagagtaag aacctgtcgg aatatcaaac agacaggttc tttatttagc atgagaaaaa
    12001 taaagttgaa ggtgcgttat attaaacgcg cttgctataa gaatatttta ctcatgagtg
    12061 agaatctggt tgtttattac ccttaaccat tatcgaccac gatattgctt ttacgtaaca
    12121 gtgggcaatc tgttatcccc aagaaaccac ttttagtgtg caagtattgt accgtgctgg
    12181 tgcctctggc agtcagatag gtacattgca aacctaatcc tgcggcattc tctttgcttc
    12241 caatcaaaac gccatatccg ctgagtaata atcctatcca taccagtgct atcagcataa
    12301 ctgtgcgaat gatgaatcgc attacaacct cttctctttt tatgttcgct taatcgtagc
    12361 ggcaatatgc gctgaagcaa gctactcatt ccgaaaaagc acgaatatcg acatagttag
    12421 gcgctgttta actaacgcat gctagtttaa tgacataagg taggtgaagc ggagattgga
    12481 gtgaaaaagt ttcgatgggt cgttctggtt gtcgtggtgt tggcttgctt gctgctttgg
    12541 gcgcaggtat tcaacatgat gtgcgatcag gatgtacaat ttttcagcgg aatttgtgcc
    12601 attaaccagt ttatcccgtg gtgatatcat tttttcagca ctagattttc ttcctctcca
    12661 gcagtggtag aatagcgcct ctactcagag gtggtgaaat gaacgaagtt gtaaattcag
    12721 gcgtgatgaa cattgcgtct ttggttgtat cggtggtggt tcttcttatc gggctcatct
    12781 tgtggttttt tatcaatcgt gccagttcac ggactaacga acagattgaa ctgcttgagg
    12841 cgttgctgga tcagcaaaaa cgtcaaaatg cactgttacg tcgtttgtgc gaagcaaacg
    12901 aaccagagaa agcagataaa aagaccattg agagtcaaaa atcggttgat gacgaagata
    12961 ttattcgcct ggtcgccgaa cgataatcct tcctcaatct tgagtaaccc cgtcgttgaa
    13021 gggttactca attcatcctg attatacgca ctgtcatggt gcaactgctt caaattaaaa
    13081 ctctggtctg acttcaaata attatcatat tatttatttg cgtaacggga tatcagaaaa
    13141 agatattttc ctcattaatt tattaggatg tttacatcgg atttgtgatt aagcgtggta
    13201 ttatttatta gcgaaacgtt tctctcttga tttttttgct cattcatcaa tttttcttat
    13261 tttaaatttt caatcccttt ggggattgac ttctctttag ggtaattgat agccgttaac
    13321 cgactgtttt atgagaaaaa gtgatataac tttttattca ttgcatagca aaaaatgtga
    13381 tattgcacgc actatgtaat aacttctccc actggcctgg aacaactgaa cttattgaac
    13441 tatgttagaa aatacgccag tttaagtatc tgcctgaact ggcaaggtta agcacaatga
    13501 tatatcggcg cgtattccgt tgcataagtg tgcaaaaaaa gtggaagacg tatcgagatt
    13561 tgtgcgtctg atcgagacat gtttaaaaat ggcttgccat aattaacgtt gtatgtgata
    13621 acagatttcg ggttaaacga ggtacagttc tgtttatgtg tggcattttc agtaaagaag
    13681 tcctgagtaa acacgttgac gttgaatacc gcttctctgc cgagccttat attggtgcct
    13741 catgcagtaa tgtgtcagtt ttatctatgt tatgcctgcg ggcgaagaaa acaatctaag
    13801 gaatttttca aatggcaaag attaaaggtc aggttaagtg gttcaacgag tctaaaggtt
    13861 ttggcttcat tactccggct gatggcagca aagacgtgtt cgtacacttc tccgctatcc
    13921 agggtaatgg cttcaaaact ctggctgaag gtcagaacgt tgagttcgaa attcaggacg
    13981 gccagaaagg tccggcagct gttaacgtaa cagctatctg atcgaatcca ctgatctgaa
    14041 gtgtgaatac gcttcaatct cgctataaag cctcgtcgaa tgcgaggctt tttactatgc
    14101 tttatcttcg ctcctggcgt tcggatattt gcccgccgcg tgattcgcgt tacacttgcg
    14161 gcctttagta tcctgccgga gttgtcatgt ctttttcctg tccactttgc catcagcctc
    14221 tttcgcgtga aaaaaacagc tatatctgtc cccagcgaca tcagtttgat atggcgaaag
    14281 aagggtatgt caatctgctg tccgttcagc ataaacggtc tcgtgatccg ggcgacagcg
    14341 cggaaatgat gcaagcacgc cgcgcattct tagatgccgg acattatcag ccgctgcgtg
    14401 atgcaattgt cgcccaactg agggaacggc ttgatgataa ggccacggcg gtgctggata
    14461 ttggctgtgg tgaagggtat tacacacacg catttgccga tgcgttgccc gaaatcacca
    14521 cgtttggtct ggatgtttcg aaggtagcga taaaagcggc ggcgaaacgc tatccgcagg
    14581 tcactttttg tgtcgcttcc agccaccgtt tgccgttttc cgataccagt atggacgcca
    14641 taatacgtat ttacgcgccg tgtaaagcag aagaattagc acgagtagtg aagcccggcg
    14701 gctgggtcat tactgccacg ccgggaccgc gacatttgat ggagctgaag gggctgattt
    14761 acaatgaagt acatcttcat gcacctcatg cagaacaact ggaaggtttt acattacagc
    14821 agagtgcgga gttgtgttat ccgatgcgtc ttcgcggtga tgaagccgtc gcattattgc
    14881 agatgacgcc gtttacctgg cgtgcgaagc cagaagtctg gcaaacactg gcagcaaaag
    14941 aagtgttcga ctgccagacg gactttaata ttcacctctg gcagcgttct tattaaccgt
    15001 ggaagtgcgt ccagaggatc tggacgccga tgccgatcag taccagcccg ccgagaattt
    15061 ccgctttttt cccaataatt gagccgataa agcgaccaac catcatccct aatgttgaca
    15121 taatcaaggt tgcacaacca atggccaatg cggtcgcgat aatgttgacc tgcaggaaag
    15181 caagaccaac acccacagcc atggcatcca ggctggtggc aatcgcggtg gttaccagta
    15241 gccagaaacc gtgtcgacgg cgcggctctt catcttcatc atctgcgcca cgaaaaccct
    15301 caataatcat tcgcccgccg aggaatatca gcagcacaaa cgcaatccag tggttccatt
    15361 caaggacaaa ccggctggct aacatgccca ttccccagcc gatcagcggc gtcagggttt
    15421 cgacggcacc aaaaataagg ccggttcgca atgcttcaga aaatttcggt ttatggaggg
    15481 tggcaccttt accgattgat gcagcaaatg catccatcga cataccaaac gcaagaagaa
    15541 cagtagcagt gatattcatg acaatgtcct gaccggggta tccataaaac acatcatcgc
    15601 ccccagcaaa catgcataca tgaacaacaa gccgtgatgg ttttcgcgtt catgcaggca
    15661 tagcgattga tgtgcatatg gtctcgcctg actgaaagta ttcagtccgt acgtgccacg
    15721 ttttgcaacg agtatgttga cacgtacatt tccggaatct ggaaatcggc tactccccaa
    15781 atgagggcgc aaccttaaca tattttgaga atataaaaca actgttggaa tatattattt
    15841 cattgcttta ttgataacga ttttcatttg gatttatata tcaatgaaac gttaaaataa
    15901 ataacaaata agggaagagg taatatagcc taagctatgt ctgattatta aaaatacact
    15961 aaacatagct ttggctaaat tcataactta ttgagttgaa acgagaagct tataaatttt
    16021 ttccagatcg tcgatatttc gaacgcggat taacagccga cgctgttcta attgcatcac
    16081 caacacgcca tcttccgaca agttcatcgc tttgattcgg ctatattcaa tccagacatt
    16141 ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga acgcggatcc agaatatata
    16201 aaaacccatc agagccagtg cgcttaataa ccatgtcgtt attaacgcac catgattcgt
    16261 gacgttgtta tagataagaa tgacaatcaa tccgacgaag ataacgctat cgatgcgacc
    16321 acgccggagc aaaggaattg ccagcagggt ggggccgtta cggcggggca tgatgaactg
    16381 atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga atcagtacca ggtccgtgat
    16441 tgtcattaat cctccagata aaaaaacggg gccaaaaggc cccggtagtg tacaacagtc
    16501 ttacagtccc agcaggccgc aagcgtaacc agcgataccg atgacgaaga agccaacgat
    16561 gatccacagc gggttaactt ttttgcgcag tagccacata caagcaaagg tcagcagcag
    16621 tggtaccagg cctggcatta actggtccag aatagtctgg acagtagtaa cgtgttcttt
    16681 gcccgtctgg tcagtaatgc gagagacaac cagcgggatg ttgacatgtg tccacttgtt
    16741 aaccaatgcc cccatgacaa acaggccgag gatagacgcc ccttccgtca gtttttgcag
    16801 gaagccacca cccatatctt taacgatatc gatacctttg gagtaaccat acgctacgcc
    16861 gtagtaacgg gttgccagac gcaccaggtt aaacaggatg aagaacagca gcggacctaa
    16921 caggctgccg ctcatcgcga taccggcacc cagtgctgca aataccggac gtacggttcc
    16981 ccagaagatc gggtcgccta caccagccag cggccccatc aaaccgactt tgataccgtt
    17041 gatagcaccg tcgtcgatct ctgcgccatt agcacgctgt tcttccagcg ccagggttac
    17101 gccgagaatc ggcgcagcca cgaacggctg ggtgttaaag aactccaggt gacggcgaat
    17161 agcttgttta cgagcttcgt tgttctcagg gtagaggcga cgaattgccg gtaccataga
    17221 gaagcagaaa cccagtgcct gcatacgttc gaagttccat gaaccctgga agaggttaga
    17281 acgcaggaag acgccacgaa tatcactttg agtgagtttt ttctcggtgg tagtttgagt
    17341 tgtatcaacc atttcgctca cctgttagtc cagttcgtta tcgagatcgt tgttaccagc
    17401 tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg ctaagttgga tgtagagcac
    17461 tgccataaca gtaccaatca cacccagagc aaccaggtta aagttggtga atgctgcggt
    17521 tacgaagccg aggtagaaga acggcatcag gtagccagca cgcatcatgt tgataaccat
    17581 cgcataacca accaccacga tcatgccacc ggcgatattc agaccattgg tcaccacttc
    17641 cggaatcgca ttcagcatgt tctgtacttc gctggtacca acagacagcg caacgatgac
    17701 ggccggaata gccacacgca ttgcttgcag gaacagagaa gaaacgtgga tccaggaaat
    17761 cgctgtcagg ttgccgttat cagcagcctt atccgcagcg tgctggaaag caacggtaat
    17821 agtacgaacg atgatggtca gtacctggcc cgcagcggcc agagggattg ccagtgcgat
    17881 acctgcacca atgctctgat gacctgcgat aaccagaatg gtagaaatga tagaagccag
    17941 agcggcgtca ggcgcaactg cagcaccgat gttcatccag cccagcgcga tcatttccag
    18001 cgtaccaccg ataataatac cggttttcat atccccaaga acgataccca ccagggtaca
    18061 cgcgattagc ggacggtgaa actgaaattc atcgaggatt gatcccatac ctgcgataca
    18121 ggctacgata aataccagca caatttgaag agtggtaatc tccattgtac ttctcctgtt
    18181 tacgtaagac ttaagtgtga aaactgagtg ataatcaaca caatacgtta cttatcgatt
    18241 ttgctgatca gatccatcat tttcagtttc ggatcggtgg aaaccttacg gacttccagc
    18301 tcaataccgc gcgcattcag tttcttgaac gcctcgatat ctttttcatc aaccgaaacc
    18361 gcgttattca cctgggtttt accctgacgg aatgccatac caccgacgtt aacagaggtg
    18421 attttcacgc cgccttcaac gagacgctct acatctgttg ggttggtaaa taacagcatt
    18481 acgcgttcgc cagcatattt cgggttgttg tagacgcgaa tcattttggc aacatcaact
    18541 acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca gtgtcttacg aacggtatcc
    18601 gcagccactt catcactaac aacaataata cgggagacat tggtttcttt ggtccagcgg
    18661 gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg caaggccaat aaccatgtag
    18721 tcgtttggcc ccattggttt tgccggagtt ggagccgctt ttggtgctgc ggcagcgggt
    18781 gctggcgcgg ctttttcaac cggtttggct ttcagtgctt tcacgccttc acggcctgtt
    18841 tctactgcca gcgcaaccag ttcatcaaag cttgggtcat catcacgagc cattaacgtt
    18901 tccacgagca ttggaatgtt aacgcctgca atgacttcat aatgctcttt gtcgacgaca
    18961 atgcggctgg cagcattgaa cgggctgcct ccccatgtat caacgagaaa cagcacgcct
    19021 ttagtggtgt cgagttttgc caactgagcg ttgtactttt caatcagcgt ttcggcattt
    19081 tcacctggaa cgaaatcgat ccagccgacg ttttcctgct cgcctaacag catttctgcc
    19141 gttttaagca actgctctgc agcccaacca tgtgtgccta taacaatagc aatggtcact
    19201 tgctacctcc tttattatcg ttaacacctc aacgtgccag atgtattttt gaatcgtcgt
    19261 ccacaatcga atcgattcag ataagggcta caaccaaaaa cccctgcgtt tcgcgattta
    19321 ttttagatat cgaaaaaatt attttatgtg atgaagatcc gtaatttaac tttcgattag
    19381 cagaaatttc gaaaggtaaa atatccttgt cacattcgtt tgcaaaggaa ggtaaatctt
    19441 tgccaaatca gaggcgtctc tgatatgttt aactcccgtt taacaaccat ggagtatagg
    19501 gcagtagccc gcagtatgga tcgtcaccga cgtcatttca gcatcaggcc ttttaacgcc
    19561 tgcctttctg gcactctatg ccgcaccttt cgtttgcatt ttgtcgttac gcctgcatta
    19621 tttctggcgt cgaatagcta ttccttaagc aggagcttgt catggaattc ttaatggacc
    19681 cctcaatttg ggcggggcta ctcacgcttg ttgttctcga aattgtgctg ggtatcgata
    19741 acctggtctt catcgccatt cttgctgaca aactgccgcc aaaacaacgc gataaagcgc
    19801 gtttgctggg gttatcactg gcgctgatta tgcgtctggg gctgctgtcg ctgatttcat
    19861 ggatggtcac gctgaccaaa ccgctattta ccgtcatgga tttctccttc tccggacgcg
    19921 acctgattat gttgttcggg gggatattct tgctgttcaa agcaacaacc gaactgcatg
    19981 aacggctgga aaaccgcgat catgattccg gccacggtaa aggctacgcc agtttctggg
    20041 tggtcgtcac acagattgtc atccttgacg ccgtcttctc gttggatgcg gtaattactg
    20101 cagtagggat ggttaaccat ctgccggtga tgatggcggc ggtagtgatt gcgatggcgg
    20161 ttatgttgct ggcatccaaa ccgctgacgc gattcgttaa ccagcacccc acggtggtgg
    20221 tgctctgtct gagcttcctg ttaatgattg gtctgagtct ggtggcagaa ggtttcggtt
    20281 tccacattcc gaaaggttac ctgtatgccg cgattggctt ctcgatcatc atcgaagtgt
    20341 ttaaccagat tgcgcgtcgc aactttattc gccaccagtc gactttgccg ctgcgagcgc
    20401 gtactgccga tgccatcctg cgtttgatgg gcgggaaacg tcaggccaat gttcagcacg
    20461 atgccgataa cccgatgccg atcccggaag gtgcatttgc cgaagaagaa cgttacatga
    20521 ttaacggcgt actgacgctt gcgtcgcgtt ctctgcgcgg gatcatgacg ccgcgcggtg
    20581 aaataagctg ggttgacgct aatctcgggg tcgatgaaat ccgcgagcaa ctgctctctt
    20641 caccgcacag tctgttcccg gtatgtcgcg gtgaactgga tgaaatcatc ggtattgtac
    20701 gtgctaaaga actgctggtg gcgctggaag agggcgttga tgtggcggcg attgcttcgg
    20761 cgtctccggc gattatcgtc ccggaaaccc tcgatccgat caacctgttg ggcgtgctgc
    20821 gtcgtgctcg cgggagcttt gttatcgtga ccaacgagtt tggtgtggta caaggtctgg
    20881 tcacgccgct ggatgtgctg gaagccattg cgggtgaatt cccggacgct gacgaaacgc
    20941 cggaaatcat tactgatggt gacggctggc tggtaaaagg cggtacagat ttgcatgcct
    21001 tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga tgatatcgcg acggtcgcgg
    21061 gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg cgatgtgatt gatgtagggc
    21121 cactgcatat caccatcatt gaagccaatg attatcgtgt tgatctggtt cgcattgtta
    21181 aagagcaacc ggcgcacgat gaagatgagt aagcattaac gtaacggcat aatgggcgtg
    21241 atatgtccat tatgccgggc gggcggcggt tggctgcccg ccagccattt gggaaaatca
    21301 cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa tatgcacgcc atgacggcgc
    21361 aaatacttcg cctgttcttg cgtttccacg ccctccgcca ccagttcaat attcagtctt
    21421 tgccccagcg cgatgatgat atcggtcacc gtcgagttaa ccgcgtcaga acctatagct
    21481 gcggtaaatg acttatcaat tttcagcatg tcaggacgta atgtttcaag ccaggaaaat
    21541 gaactgttgc cggtgccgaa gtcatcaatc gccagtttga cgtttttacg atgtagctcg
    21601 cgggcaatcc gataatcaac atccagtaag gcatcgcgtt cggtgatttc gaggatcagt
    21661 tgctgaatcg ggtgagcgct aaaccagtac tgattgagat cttttatcag cacaccacgg
    21721 cgaaaatggc tgggtgcgac gttaatgcca acatgaaact gactactcat cgggaaaaca
    21781 tggcgctgac gaatggtttc tgccatcaca tagcgggtca gtggcacaat taaatgatgt
    21841 tcttccgcga taggaataaa cacatccggt gaaatccagc cctgacgcgg attgttccag
    21901 cgcagcaaaa tctctacacc aatacattgc tggctgcgcg cattaagcaa aggctgacag
    21961 aacaattcga actcatgttg cgccaggccc agattgattt cgcgggaaaa gctcatccgg
    22021 taagcggtcg ccagccaggc gatgtagccc accagtaaac ttagcagcac cgccagcggt
    22081 aattgtgttg gaagatagtg ccatgccagc gccgtagccc ccggaccatt aacgttaatg
    22141 gtaaaaggaa agcgttgcga agaaacctga tagttttcat tgtcttcagg ttgcggaagg
    22201 gaatctacca gcccattacc atagagcaaa tgccgtttgt ccaccgtcag gctggcgcta
    22261 ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca gtaagtcgat gttaatcatc
    22321 tccatgaccc cagcattgct actgcccgct gcaggcgtcc attgaatcaa aaccggactg
    22381 cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg gttgcggtgc aggaagttca
    22441 gccagaatgt ccacgacggg cacattgcga taaccaaaaa tgctggagca atagagtgtt
    22501 ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt ttgccgcctg tttacgtaat
    22561 ggcaaatggg cgacagagca gggcagacca atcagcggaa gcaagacttc gctacctgcc
    22621 tgtaggggaa gcagtacttt atctaattcc tctacagcgt gattggcgaa ttgtactacc
    22681 cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc tcagggtcga agcgagcgta
    22741 acgagtgcgc aaatcgtaca aacaatcatt cgattacggc gataggtttt aatgatccgt
    22801 tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg acaaacgcct gacgcaacag
    22861 tggaagtgta gtggggaata ttacagcaga cgagaaagcg ggtataaatt cgcccatccg
    22921 ttgcagatgg gcgagtaaga agtattagtc acactggact ttgattgcca gaccaccgcg
    22981 tgaggtttcg cggtatttgg cgttcatgtc cttaccagtt tcgtacatcg tttcgatgac
    23041 cttatccagc gagacgcgcg gtgcactggt gcggcgcaga gccatccgtg cggcgttaat
    23101 cgccttcaca gaggcaatgg cattacgctc aatgcacggc acctgaacct gccctgcaac
    23161 cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt tccgccgcca cgcaaacctg
    23221 ttccgggcta ccgcccagca gttctgcaag acccgcagca gccattgaac aggcaacacc
    23281 cacttcgccc tggcaaccaa cttccgcacc ggaaatagag gcgttcattt tatacaatgc
    23341 accaatcgcg cccgctgcca taaagtaacg ggtatagatg tccgggctga ccgattcaat
    23401 aaagtggtca tagtaagcca gcactgccgg aacgataccg caggcaccgt tggttggcgc
    23461 agttaccaca cgaccaccgg cggcgttttc ttcgttaact gccagcgcaa acatgtttac
    23521 ccagtcaatg acattcatcg gatcgttaga cagtttatcg ctggaaacta acatccggcg
    23581 cagggcagac gcacgacgtg gcacgcgcag cgggcctggc agtacacctt cggtgttcat
    23641 cccgcgatcg atacatgcct gcatggtttg ccagacatgc gcgaaatact cgtcgatctc
    23701 tttcttgctg tgcagcgcca gttcgttctg catagcgaga ccagacagcg aatagccggt
    23761 ttcattacag tacgcgagca gttcggtggc agatttgaac ggatacggca cgctcacttc
    23821 gttggcagca tcctgaccaa agtgttcttc atcgacgata aaaccgccgc cgatggaata
    23881 ataagttttg ctgtagacga cttcatcgcc gttataggcg tggatttgca taccgttttc
    23941 atgcagcggc aggttgccgt tatgaaaacg catcccgttg tcgcgcggga aatccacttc
    24001 atgccgtccc tgtgccagca gcagacgttc gcgctcttct acgtcgcgaa taaaaccggg
    24061 aatactgtcg atatccacgg tggcaggttc gttacctgca agacccataa taatggcgat
    24121 atcggtgtgg tggcctttac ccgtcagcga cagtgaacca taaacgtcca cggcaacgcg
    24181 agtaacgcta tccagtaagc ctttttcgac cagatcatcg acgaactgtt tacctgcctt
    24241 cataggccct acggtatggg aagatgaggg accaatcccc accttaaaca tgtcgaatag
    24301 actaatcacg ataatactcc tgacaaggtg actggacttc cagtaacgaa ctaataacag
    24361 cgcatagtgt aagagggaac gcggcgactg gcttaactat tcacatgaat taaactaatc
    24421 aaaaaccgcg atttaactaa tattgcgatg attgtctcaa gtagtgtttt gtagatgtaa
    24481 agataagtat agtcagggtt tcacaccaat ttgcagcgcc agctcacgaa ttatgcctgc
    24541 ggtcattccc catacaaaat actgttcgta ccaggacagc catacccgat gtgaatcgcc
    24601 acggcggtag atatctaaag ggtgataacg acccagatgt aatgcctggg cgagcggcat
    24661 ttcaaacacc gccgagactt catcttcact ggcgcgatac ggcagatcgg gcgggataat
    24721 gccgaccact ggggttacct gatagccagt tacgctatcg acgggcggca gcacgccgat
    24781 aacttcaacg gcggaaggcg gtatagcgac ctcttcttca gcttcgcgca gcgcggcggc
    24841 gataactgat gcgtccgtgt catcgactgc acctccaggg aatgccactt gtccagcgtg
    24901 tttacgcaga tgaattgaac gctgagtcag caacaacccc ggttgcggtc gacggacgat
    24961 ggggattaac acggcagcct gacgatgatt tagggtttcc cggttaattt gcgggcgcaa
    25021 aagttgaaag cgcgataaaa aatcatcaag catcaggcta cggtattcca cgtcttactt
    25081 ctccagttgg cgtaatatct tattaacttt atcaaaagtt tcctgatatt ccgcttcttc
    25141 ctggctatcg gcgacaattc caccgcccgc agagcagtat atttgtccgt taatggcagt
    25201 cagagtgcgg atagtaatgc tggtatccat gttgccgcaa aagctcaaat agccaatgct
    25261 gccgcaccag gcattacgtc gctgcggttc cagttcgtcg ataatttcca tagcccgtac
    25321 tttcggagcc ccggttattg agccaccagg aaaagctgcg cgcagcagat cgctggcgtg
    25381 taactgttct ggtagttgtg ccgttatggt gctgaccaga tgatgcacgg cagggaaggg
    25441 ttccaccacg aacagctctg gtacttttac cgaacctgct acggcaacac gaccgatatc
    25501 attacgcatt aaatcgacaa tcatcagatt ttcggcacga tctttcgctg agttcgccag
    25561 tttttctgct tgtttgctat cttcctgagg atcgggcagg cgtggtagcg tgcctttaat
    25621 agggcgggtc tggatttcac tattatcaca aagaataaac cgctctggcg aaaggcttaa
    25681 aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc gcgcggttgg cctgattaag
    25741 ctgaaggaat gcctgccatt catcgccaga ataggtcgca tgaaaacgct gggcgagatt
    25801 tacctgatag caatcaccgc tgtgcagata ttcctgtacc tggcgaaatt tttcgccgta
    25861 ctgctcgcgg gtcatattgg attgccagtc ggaagtaagc gtgaaatctt cctgcggcga
    25921 gaattgctgg ctttccagcc aggcccgacg ggcattgaca tcattatgac tcagcaaaga
    25981 aactgtatga cgctggtggt cgacaatgag cgcccaatcg tagataccca ctgccatatc
    26041 cggcagaacg atatcttgtt ccgcaatttc tggcagtgac tcaaaacggc ggcccagatc
    26101 gtagccaaac aaccccagtg cgccgccctg aaatggcaaa tcttcgttat gcgttgggcg
    26161 aatgtctgcg cgatccagca cctgctggag cacctgtagc gggtcatcag tggtcgttgt
    26221 gcgtttttcg ctttcactaa caacggtttc tttacctaaa gtggttaaag tgcaaatcgg
    26281 atcggcgacc acaatatcaa agcggctata cggatgatcg gcatagccgg agtgtaaaag
    26341 catcgcccac ggcaggtggc ttaagcggga gaaataaaat tcagcggcgt cctgacgcca
    26401 gggtaaagta atcacagcgg gagataacgt cttcatcagt cctgactcta ctggctatgt
    26461 gccgggtagc atgaaataat tagcgccaac aatttagcag gagttaacaa tgtttgcagg
    26521 tttaccttca ctcacccatg aacagcagca aaaagctgtc gagcggatcc aggaactgat
    26581 ggcacaaggg atgagcagcg gccaggcaat cgcgttggtg gcagaagagc tgcgtgccaa
    26641 ccattccggc gagcgaatcg tggcgcgttt tgaggatgaa gacgagtaac acactaatcc
    26701 gtattgattt cgttatacaa gccgatgatt atttgattgt gagaaaaagg acgcttttta
    26761 tatgtgatta aaagcgtcct gatccattta tgctgatgta aattttactc agaccagtgc
    26821 ttttagttct tcctgcatat atgttgaaaa gtattcagga tttttaatca cagagcgttc
    26881 acctgactcc attttttttg cccatccaac cattttttcc gtaaacccag ggttccagcc
    26941 ttcctggtca gctcgcgccg taatatcagc ggcactcgc
//
LOCUS       DAJTYZ010000048        26887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000048 DAJTYZ010000000
VERSION     DAJTYZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.048"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            110..382
                     /locus_tag="OO596_001933"
     CDS             110..382
                     /locus_tag="OO596_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6717797.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            475..558
                     /locus_tag="OO596_001934"
     CDS             475..558
                     /locus_tag="OO596_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717798.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            658..1479
                     /locus_tag="OO596_001935"
     CDS             658..1479
                     /locus_tag="OO596_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6717799.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1518..1847)
                     /gene="mdtI"
                     /locus_tag="OO596_001936"
     CDS             complement(1518..1847)
                     /gene="mdtI"
                     /locus_tag="OO596_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6717800.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1834..2199)
                     /gene="mdtJ"
                     /locus_tag="OO596_001937"
     CDS             complement(1834..2199)
                     /gene="mdtJ"
                     /locus_tag="OO596_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6717801.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2611..3645
                     /gene="tqsA"
                     /locus_tag="OO596_001938"
     CDS             2611..3645
                     /gene="tqsA"
                     /locus_tag="OO596_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6717802.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3670..5058)
                     /gene="pntB"
                     /locus_tag="OO596_001939"
     CDS             complement(3670..5058)
                     /gene="pntB"
                     /locus_tag="OO596_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6717803.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5069..6601)
                     /gene="pntA"
                     /locus_tag="OO596_001940"
     CDS             complement(5069..6601)
                     /gene="pntA"
                     /locus_tag="OO596_001940"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6717804.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7125..8069
                     /locus_tag="OO596_001941"
     CDS             7125..8069
                     /locus_tag="OO596_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6717805.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKTDAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8255..9637
                     /locus_tag="OO596_001942"
     CDS             8255..9637
                     /locus_tag="OO596_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6717806.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9674..10396
                     /gene="folM"
                     /locus_tag="OO596_001943"
     CDS             9674..10396
                     /gene="folM"
                     /locus_tag="OO596_001943"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6717807.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10393..10728)
                     /locus_tag="OO596_001944"
     CDS             complement(10393..10728)
                     /locus_tag="OO596_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6717808.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10857..11576
                     /gene="rstA"
                     /locus_tag="OO596_001945"
     CDS             10857..11576
                     /gene="rstA"
                     /locus_tag="OO596_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6717809.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11580..12973
                     /gene="rstB"
                     /locus_tag="OO596_001946"
                     /pseudo
     CDS             11580..12973
                     /gene="rstB"
                     /locus_tag="OO596_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="OO596_001947"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="OO596_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6717810.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="OO596_001948"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="OO596_001948"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6717811.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="OO596_001949"
     CDS             complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="OO596_001949"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6717812.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17366..18541
                     /gene="manA"
                     /locus_tag="OO596_001950"
     CDS             17366..18541
                     /gene="manA"
                     /locus_tag="OO596_001950"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6717813.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18642..20150
                     /locus_tag="OO596_001951"
     CDS             18642..20150
                     /locus_tag="OO596_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6717814.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="OO596_001952"
     CDS             complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="OO596_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6717815.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="OO596_001953"
     CDS             complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="OO596_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6717816.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="OO596_001954"
     CDS             complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="OO596_001954"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6717817.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="OO596_001955"
     CDS             complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="OO596_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6717818.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="OO596_001956"
     CDS             complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="OO596_001956"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6717819.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6743 a         6970 c         6470 g         6704 t
ORIGIN      
        1 ccattcccag ggatatagtt atttcaacgg ccccgcggtg gggttaaatg aaaaaacaaa
       61 ttgagggtat gacaatgaaa aagtattagc tctggttgtt gccgctgcta tgggtctgtc
      121 ttctgccgcc tttgctgcag agactgcgac cacacctgct ccgactgcga cgaccaccaa
      181 agcagcgccg gcgaaaacga cacatcataa aaaacagcat aaagcagcac ctgcccagaa
      241 agcgcaggcg gctaaaaagc atcataaaaa tacgaaagct gaacagaaag cccctgaaca
      301 aaaagcgcag gcagcgaaga aacacgccgg gaaacacagc catcagcaac cggcaaaacc
      361 tgctgcacaa cccgcagcgt aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg
      421 aaaattacgg tgctaagcgg gtaacgttta gcaccgcctt tagcggaggg tagtatggtg
      481 ggccgttatc gctttgagtt cattcttatc atccttattt tatgcgcact gattgccgcc
      541 cgtttttatc tttcctgact gtagttatct gattttactc ccactttcat cccgtcccgt
      601 ctatagtatt tatgagggtt tgcttttaat aatcataatt acccaccaga gtgtgatatg
      661 cgtacaacca ttgctgtagt gttgggtgca attagtttga cgtctgcttt tgtgtttgca
      721 gataaaccag acgttgccaa atcggcaaac gatgaggtca gcaccctgtt ttttggtcat
      781 gatgatcgtg tgccagtgaa tgacacgacc caatcaccgt gggatgcggt tgggcaactg
      841 gaaacggcca gcggcaattt atgtacggcg acgctgattg cacccaatct ggcattaacg
      901 gcaggacact gtttattgac acctccaaag ggtaaagccg ataaagcagt ggcgctgcgt
      961 tttgtgtcaa ataaaggtct ttggcgctat gagatccacg acatagaagg ccgcgttgat
     1021 ccgacactgg gaaagcggtt aaaagcagat ggggatggtt ggattgtacc tcccgcagcc
     1081 gcgccgtggg acttcggatt gattgtgcta cgtaatcccc cttctggcat tacaccgttg
     1141 ccgttatttg agggagataa agccgcgctt actgccgcat taaaatcggc tggccgtaaa
     1201 gtaactcagg cgggataccc tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt
     1261 gaagtgactg gctgggcgca aacgtcggtg atgtcacatc agtgcgatac cttgccgggt
     1321 gacagcggtt cgcctctgat gttgcatacc aatgacggct ggcaattaat tggggtgcaa
     1381 agttcggctc ctgccgcgaa agatcgctgg cgcgccgata accgggccat ttctgttacc
     1441 ggttttcgcg acaagctgga tcaactgtcg caaaaataat gttcaaacgc tgcccgactg
     1501 tgcgggcagc gtgttcatca ggcaagtttc accatgatca ttccagccaa cagcaagacc
     1561 aggccaatcc agcctttacg atttaaccgt tgaccaaaca agatccaacc tgcggctaac
     1621 gtggcggcaa taccaaaccc gccccacaat gcataagcga cagacaagtc gatcccttta
     1681 acggcctgag aaagcgcact aaaggcagcc agcaccgccg ccagggagag caagccaaat
     1741 attttgcgac gaaagccgtc agaaaatttc aaaaagacgt tagcaacgat ttccagcacg
     1801 attgccaatg ccagccaggc ggcgtgaacc cattcaaact gcgccatggt tcacctccag
     1861 ttcaggttta cgcgctttac gggtacctga ttttatcaac acaatcccgg cgaccagggt
     1921 ggttaacccg gcaattttca tcagcgataa actttcgtcg aataacaaaa cgctaaacaa
     1981 ggtaataaat aaaataccga taccttccca cagcgcataa gctacgccta aggcgatctt
     2041 tttaacggcg aaagagagaa atatatacga cagagaaatc atcaccagca ttaaaataaa
     2101 gccgccattt ccctcactga cgctcgccca tttcattgac agcgtaccgg taatttctgt
     2161 agcaatagcc aggcctaata aaatccaata aatatacatt gtccttctcc tgcaagagaa
     2221 ttattttaat tttcgcttaa ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgcata
     2281 gggcaagctt aggcagaaga aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga
     2341 gcaagatagt ggatgaggta cgaagatggg taaatgttga aaataacgtc ctgaacaaat
     2401 tgtccataat attacaatta tccgcagtgt tgcttctcgt catcgcggat gataattgtc
     2461 ctcggtagtt gaacacgcct gatttgtatc atagcttaag aattaactca aaatattttc
     2521 acttctttac ctgagcggtt tgattttcgt tatgatgacg gagcgaaaaa gacattatta
     2581 ttagcaaagg aagaaaaaac ggggacaagc atggcaaagc cgatcatcac gctcaatggc
     2641 ctaaaaatcg tcattatgtt gggaatgctg gtcattattc tctgcggtat ccgttttgcc
     2701 gccgagatca tcgtgccgtt tattctcgca ttatttattg ctgttattct taacccgctg
     2761 gtgcaacaca tggtccgctg gcgagtgccg cgtgtactgg cggtgtcgat tttgatgacc
     2821 atcatcgtga tggcgatggt gttgctatta gcttatctgg gttccgcgct caacgagttg
     2881 acgcggacgt taccgcaata tcgcaactct attatgacgc cgctgcaagc tcttgagccg
     2941 ttgttgcaac gcgtagggat tgacgtctca gttgaccagc tggcgcatta cattgatccg
     3001 aacgcggcga tgacgttgct caccaactta ttgacgcagt tatctaatgc catgtcatca
     3061 atatttttat tgctgctgac ggtgctgttt atgctgctcg aagtgccaca attgcccgga
     3121 aaatttcagc aaatgatggc gcgtccggtt gaagggatgg cggcgattca acgtgcaatt
     3181 gacagtgttt ctcattatct ggtgctgaaa acagccatca gcatcatcac cggcctggtc
     3241 gcctgggcga tgctcgccgc actcgatgtt cgcttcgctt ttgtctgggg attgctggcc
     3301 tttgcgctta attacatccc taatattggt tcagttctcg cggcaatccc ccctatcgct
     3361 caggtactgg tgtttaatgg cttctacgaa gcgttgctgg tgctggcggg atatctgctg
     3421 attaatctgg tcttcggcaa tattctggag ccgcgcatca tgggacgtgg gctggggctt
     3481 tccacattgg tggtattttt gtcgttgatt ttttggggat ggttgttagg accggtgggt
     3541 atgctgattt ccgtgccgtt aacaattatt gtcaaaattg cgcttgaaca aacagcggga
     3601 ggtcaaagca tcgccgttct gttaagcgat ctcaataaag agtgacggcc tccgcagagg
     3661 ccgtcagggt tacagagctt tcaggattgc atccacgcta gctttggcgt caccaaacag
     3721 catgtgggtg ttttccttga agaacagcgg gttttgcaca ccagcatagc cagtgttcat
     3781 cgaacgttta aagacaatca cgttctgcgc tttccacact tccagcacag gcataccagc
     3841 aataggactc ttcggatcat cctgcgccgc cgggttaacc gtatcgttag caccaatcac
     3901 cagtacggta tcggtatcag caaagtcatc gttgatctcg tccatttcca gcacgatgtc
     3961 atacggtact tttgcttcag ccagcaatac gttcatatgt ccaggcaaac gccccgcaac
     4021 cgggtggata ccgaaacgca cgttgatacc acgagcgcgc agtttctcgg taatttcagc
     4081 gacaggatat tgcgcctgcg cgactgccat gccgtacccc ggagtaatga tcactgaatg
     4141 ggagtttttc agcagttccg ctgtctcttc tgcggtgatt tcgcggtgct cgcccacttc
     4201 ctgatcatcg ccagtagaag agccgtcggt gccgaaacca cccgcaataa cgctgataaa
     4261 ggaacggttc atcgccttac acataatgta agaaaggata gcccccgaag aaccgaccag
     4321 cgcaccggtc acaatcagca ggtcgttgct gagcataaag cccgcagccg cagccgccca
     4381 gccggagtac gagttcagca tcgacaccac cactggcata tctgcaccac cgatggaggc
     4441 gactaaatgc cagccgaata ccagcgcaat ggcggtcatt attagcaatg ccagcacttg
     4501 caggccgacg ctgtccgtgc gaacaaatac aatcagcagc aggaaggaaa cgaccagagc
     4561 cgccaggttc attttgtgac ggtttggcag catcaacggt ttagacgaaa tcttgccaca
     4621 cagtttgccg aacgccacca ccgaacccgt gaacgttacc gccccgatga agataccgag
     4681 gaacacttcc gtcaggtgaa tattgaccag aatcggtgcc attcccgcgt catgatgcag
     4741 atagctgtta aagccaacca gcactgccgc cagacccacg aagctgtgca ggatcgccac
     4801 cagttctggc atttcggtca tttcaacttt cttcgccaga cggataccaa ttgccccacc
     4861 aatgaccatc gccagcaaga tccagccaac attacccgta tccggtccaa aaatggttgc
     4921 gattaacgca atcgccatcc cggcgatacc gaagttgtta ccctggcgag acgtttcatg
     4981 tttcgaaaga ccggccagac tgaagataaa caggatcgcg gcaacaatgt atgcagctgt
     5041 aactaatcct ccagacatat gttacccctt aatttttgcg gaacattttc agcatgcgct
     5101 gagtcacggt gaagccaccg aaaatattaa tgctggctat aagcaccgcg ataaaactaa
     5161 ggaagctaac ccagccgccc tggccaatct gcaacagtgc tccgacaaca ataatccctg
     5221 aaatcgcgtt ggtgaccgac atcaacggtg tatgcagcgc gtgcgataca ttccacacca
     5281 cgtaataacc gacaacgcag gccagcgcga aaacggtgaa gtgcccaaga aactctttcg
     5341 gcgcaacgct tgccagccaa ccgaaaagga tgattgccag cgccatcaac gcgtatttac
     5401 gccacggtga gcaggcacat ttttcctcag ttttcacttc cggtgccgct ttttgtgccg
     5461 cctgcggctg agctgatacc tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga
     5521 tcacggtcac gccgcgaatc accacatcat caaaatcaac agtgatattg ccgtctttct
     5581 ctttgcacaa cagtttcagc agattaacga ggttagtacc gtaaagctgc gaggattgcg
     5641 tcggcagacg gcccggaaga tcggtataac caatcacttt gacaccattt tccgtagtga
     5701 agatttcacc cggcacggtg tattcacagt tgccaccgtt ttgggctgcc aggtcgacaa
     5761 tcacactgcc cgccttcatg gagtcaacca tttcacgggt aattagcttc ggcgctggtt
     5821 tgcctggaat aagcgcggtg gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga
     5881 gttccatttc cgctttaatg aacgcgtccg acatcacttt ggcatagcca tcgccactgc
     5941 ccgcttcctc tttaaaatcc agctcgagga attccgcgcc catactttga acttgttctt
     6001 tcacttccgg acgggtgtcg aatgcacgca caatcgcgcc gagactgttt gctgcgccaa
     6061 tggcggccag acctgcaaca cccgcaccaa tcaccatcac ttttgccggt ggcactttcc
     6121 cggccgcagt aatttgcccg gtaaagaagc gcccaaattc atgtgccgct tcaacaatgg
     6181 cgcgataacc ggcgatgttc gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga
     6241 tacgcggcac agaatccatc gccatcacgg tcacgttacg ttccgcaagt ttttgcatta
     6301 attccggatt ctgcgcaggc cagataaaac tcaccagcgt tgtcccagga ttcagtaacg
     6361 caatttcatc atctaacggc gcattgacct tcagaatgat ctctgactgc cagacgctat
     6421 tcccttctac aatttcagcg cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt
     6481 gacccgcgcc gctctctacc gcgacggtaa aacccagttt cagcagctgt tccactgttt
     6541 ttggcgttgc tgcaacacgg gtttcattgg ttaaccgttc tcttggtatg ccaattcgca
     6601 tgatattccc ttccatcggt tttattgatg atggtttgcc tgtgtcagga gccacacaag
     6661 ccgctcatgt acgagctaaa tgttactccg ttaaaataaa ttagtaacaa acgccctata
     6721 acgtactgaa aattatgcct gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg
     6781 caaaatatta acgattcagc cgtgatagtg ggataaacac cttagaacgc cggataaaga
     6841 ctgataattg tcttcgacgg ccgggtaaaa cgagacatcg ccccggcacg attcactact
     6901 taacattaaa ttaacttata caattcagtt gcttcagtag taatgatgct gatacggctg
     6961 ttttttaagc atagacggtc atttgagcag gattaaaatt ggcttaagga atgtgatatg
     7021 aaaaatgacg cagacagtta caccgtttaa atgcaataat cagccacgtt tctcgttaat
     7081 aacaatacca gtacctggtt tgcgcaaggc gaaggattat ttttatgaag cttaagaaca
     7141 ccctcctggc gtcggcactg ctttctgcta cggcattctc cgttaacgca gcaacagaac
     7201 tgacaccgga gcaagcggca gcggttaaac cttttgaccg tgtagtggtt accggtcgtt
     7261 ttaatgctat tggcgaagcg gtgaaagccg tttctcgtcg cgcagataaa gaaggtgccg
     7321 cctcttttta tgttgtcgac acttctgatt ttggtaacag cggtaactgg cgtgtggtcg
     7381 ctgacctcta taaaaccgat gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg
     7441 gtgttgtcga actgccgaaa gatcaggctg ttctgattga accgtttgac acggtcaccg
     7501 tccagggctt ctatcgtagc cagccagaag tcaatgatgc catcaccaaa gcggcaaaag
     7561 cgaaaggtgc ctactctttc tacatcgttc gtcaaatcga tgccaaccaa ggcggcaacc
     7621 agcgtattac tgcattcatc tataaaaaag atgctaagaa acgtatcgtc cagagcccgg
     7681 atgtgatccc ggcagattcc gaagcaggac gtgcagctct ggctgccggt ggcgaagccg
     7741 cgaagaaagt tgagatccta ggtgttgcga ctaccgcatc accaagttct gaagtcggtc
     7801 gtttctttga aacccagtca tcaaaaggcg ggcgttacac cgtcatgctc ccggatggca
     7861 ctaaagtcga agaactgaac aaagcgaccg cagcgatgat ggtcccgttc gacagcatca
     7921 aattctctgg caactacggc aacatgaccg aagtctccta tcaggttgcg aaacgtgccg
     7981 cgaagaaagg tgctaagtat taccacatca cccgccagtg gcaggaacgt ggtaataacc
     8041 tgaccgtcag cgcagatctg tataaataac tattcagtta ctcctataag gcggcttgat
     8101 gccgcctttc gcatttttag tcacaaattt ccccgcgacc attgcatgca gacacatctc
     8161 ttcgtaaaat cccgcgcttc agtggcaaac tccattttta tgcgttttgc cctaatattt
     8221 attcttctta tcacgtttta atcactggat atcgatgaaa aagaaactgg gactgagcgc
     8281 actcaccgcg ctggtattaa gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa
     8341 tatggcggca gttgccagcc cggcagcact gctcatcggc tggggtatta ctggcgctgg
     8401 cattttattg ctggcctttg ccatgctgat cctcacgcgc attcgtcccg aacttgacgg
     8461 cggtatcttt acctatgccc gcgaagggtt tggcgagcta atcggctttt gttccgcatg
     8521 gggatactgg ctgtgcgcag tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc
     8581 gttaagcttt tttacggaca cgccggaatt gcgcctgttc ggcgatggta atacctggca
     8641 atcgatcgtc ggtgcatcgg ctttattgtg gattgttcac tttttgatcc tgcgcggggt
     8701 gcaaaccgct gccagcatta acctggtggc gacactggca aaattgttgc cgctgggtct
     8761 gtttgttgtg ctggcgatga tgatgttcaa actggatacc ttcaagctcg acttcaccgg
     8821 acttgcactt ggcgtacccg tttgggaaca ggtgaaaaac accatgctga tcaccctgtg
     8881 ggttttcatt ggtgtggaag gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga
     8941 tgttggcaaa gcgacactgc tggcggttct ctccgctctg ggcgtttact tgctggtaac
     9001 gctgctttcg ctgggtgtgg tggcacgtcc tgaactggct gaaattcgta acccgtcaat
     9061 ggccggtctg atggtggaaa tgatgggacc atggggcgaa atcatcatcg ctgccggttt
     9121 gatcgtttcc gtttgcggcg cgtacttaag ctggaccatc atggcggcag aagttccgtt
     9181 cctggctgcc actcataaag cattcccacg catttttgcg cgccagaacg cacaagctgc
     9241 gccatccgcc tcactgtggt tgaccaacat ctgcgtgcaa atttgtctgg tactcatctg
     9301 gctgaccggt tccgattaca acacgttgct gaccatcgcc tcagaaatga ttctggtgcc
     9361 ctatttcctg gtcggcgcgt tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt
     9421 aggtgtcggt gcctgcattt atggcttatg gttattgtac gcctctggtc caatgcactt
     9481 gctgttatcc gttgttctgt atgcgccggg gctgctggtt ttcctgtatg cgcgcaaaac
     9541 gcatacacat gataatgtac tgaaccgtca ggaaatggtg ctgattggca tgctgttaat
     9601 tgcctcagtt ccggcgacct ggatgctggt gggataacgt tcatcccatc gttggatagc
     9661 aaaggagata acgatgggta aagcccagcc cttgccaata ttaattactg gcggaggtcg
     9721 tcgcatcggc ctcgccctcg cctggcattt cattaatcaa aagcaaccgg tgattgtcag
     9781 ctatcggacg cactatccag ccattgatgg actgattaag gcaggtgcgc agtgtattca
     9841 ggctgatttt tcgaccaacg acggtgtgat ggcgtttgcc gatgaagtac taaaaagccc
     9901 ccatggtctg cgtgctattt tgcataacgc cagtgcgtgg atggcggaaa aaccgggtgc
     9961 gccactggcc gacgtactgg cttgcatgat gcagatccac gttaataccc catacctgct
    10021 caaccatgcg ctggaaagat tactgcgtgg gcacggacac gccgccagcg atatcattca
    10081 ctttaccgat tatgtggtgg agcgcggtag cgacaagcat attgcgtatg ctgcaagcaa
    10141 agcggcactg gataatatga cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt
    10201 gaactctatt gctccatcgc tgatcctgtt taatgaacat gatgatgccg aatatcgaca
    10261 acaggcgctg aataaatcac tgatgaaaac cgcgcctggc gagaaagaag tgatcgacct
    10321 ggtcgattac ttacttacca gttgctttgt caccggacgc agtttcccac ttgatggcgg
    10381 tcgtcatctg cgttaatgca gctttatcca gcatataatc aacacccatg cgcttatccc
    10441 ccaacacgaa acagaaccaa caaaagcggc gggcaagcgc atcattccgg tgaaatacca
    10501 cagcgacacc aggtaaacaa aatagggaat aatcgaccac atactaaaaa tgatggttgc
    10561 gcgtaaggct tcaatgccgc gttcgctggc aacaatataa tgcgcgataa gcgcaaaggt
    10621 cgggaaaagt ggaatcagcc cggcgatata ataatttttc gtttttgcta aaacaccaat
    10681 caacagcact accagcgcac cgagcgcggc tttgattacc agccccattt ttttacctta
    10741 acacttccat aacaagtcat cagtagaata cctgatgaaa acttgtttag aaacgattga
    10801 tagtaagtaa aaacagcgcg gtgtattgtg acgtttttat atctaccgtg aatgttatga
    10861 acactatcgt atttgtggaa gatgatgcgg aagtcggttc actgattgcc gcgtacctgg
    10921 caaaacatga tatgcaggtt accgtagagc cgcgcggcga ccaggccgaa gaaaccattt
    10981 tgcgagaaaa tccggatttg gtgttactcg acatcatgct accaggcaag gacggcatga
    11041 ccatttgtcg tgatttacgc gcaaagtggt ctggaccgat tgttcttcta acctctctcg
    11101 atagcgatat gaaccacatc ctggcactgg aaatgggtgc ctgcgactat attctcaaaa
    11161 cgacgccccc tgctgttttg ctagcgcgtt tacgtttgca tttgcgtcag aatgagcaag
    11221 ccacactgac caaaggtctt caggaaacgt ctctgactcc ctacaaagcc ctgcatttcg
    11281 gcacgttgac catcgatccc atcaaccgcg tagtcaccct ggctaacact gaaatctcgc
    11341 tctcgacagc tgatttcgaa ttattgtggg aattagctac ccatgccggg caaatcatgg
    11401 accgcgatgc attgctgaaa aatttacgcg gcgtcagtta tgacggactg gatcgtagcg
    11461 tggacgtggc tatttcgcgg ttaagaaaaa aactgctcga taacgccgca gaaccttatc
    11521 gcattaaaac tgtgcgtaac aaaggctatc tttttgcgcc tcatgcatgg gaataagcga
    11581 tgaaaaaact gtttatccag ttttacctgt tattgtttgt ctgcttcctt gtgatgtctc
    11641 tgctggttgg gctggtgtac aaatttaccg ccgaacgcgc gggcaaacag tcgctggatg
    11701 atttgatgaa cagttcactg tatctgatgc gcagcgaatt gcgtgagatc cccccacacg
    11761 actggggtaa gacgctgaaa gagatggatt taaatctctc tttcgatctg cgtgtcgagc
    11821 cactgagtaa ataccatctt gatgatattt ccatgcaccg actgcgtggc ggcgaaattg
    11881 tcgccctgga cgatcagtac acgtttttgc agcgtatccc gcgcagccac tacgtgctgg
    11941 cagttggtcc tgttccttat ctttattatc tccatcagat gcgattgctg gatatcgccc
    12001 tgatcacttt tattgctatt tccctcgcct ttccggtgtt tatctggatg cgtccgcact
    12061 ggcaggatat gttaaaactg gaagcagcgg cgcaacgatt tggcgatggg catctcaatg
    12121 aacgtatcca ctttgatgag ggttcgagct ttgaacgact tggtatcgca tttaaccaga
    12181 tggcggacaa tatcaacgcc ttgattgcca gcaaaaaaca gcttattgac ggtatcgctc
    12241 acgaactgcg aacaccgtta gtgcgcctgc gttatcgact ggagatgagc gataacctga
    12301 gcgccgccga atcccaggcg ttgaatcgtg atatcaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg gcggctctgt ttatttcaca cagcgagtgc
    15541 actgtgtgag ttgtatctgc tggaagaagt catttccttt atcatccaca aggataaacg
    15601 ccgggaaatc ttccacttca attttccaga tggcttccat tcccagttcc ggatattcaa
    15661 cacattccag gctcttaata cttccctgcg ccaatacagc ggccggacca ccgatactgc
    15721 caaggtagaa gccgccgtgt tttttacagg catccgtcac ctgctggctg cggttgcctt
    15781 tcgccagcat gatcatactt ccgccctgcg cttgcagttg atcgacataa gaatccatcc
    15841 gtccggcggt cgttgggcca agagaaccgg aggcataacc ttccggcgtt ttggccggac
    15901 ccgcgtagta aatcggatga tctttaatgt actgcggcag cccttcaccg ttatccatcc
    15961 gctctttcag tttggcgtga gcaatatcac gaccgacgat aatcgtgccg ttaagcgata
    16021 agcgtgtaga aacgggatac tgcgacaact gtgcgaggat ctctttcatc ggacggttaa
    16081 ggtcaacgcg caccgcttcg ccttctcccg ctttacgcag ctcttcaggg atatatttac
    16141 ctggattatg ttccagtttt tcgatccaga tcccctgacg gttgatcttc gctttgatat
    16201 tacggtcagc agagcaggaa acacccatac caaccggaca ggatgcgccg tgacgtggca
    16261 ggcgaatcac gcgtatgtcg tgagcgaagt atttaccacc aaactgcgca cccagaccga
    16321 gattttgcgc ttcgatcagc aattcttttt ccagttccac atcgcggaat gcctgaccgt
    16381 gctcattccc ttccgttggc agttcgtcat agtatttagc ggaagccagt ttcactgttt
    16441 taaggttagt ttctgcagaa gttccaccaa ttacgaacgc aatatgatac ggaggacagg
    16501 ccgccgtacc cagcgtgcgc atcttctcaa ccaggtaatt tttcagtttc cccggcgtta
    16561 gtaacgcttt ggtttcctga tagagatacg tcttgtttgc cgaaccacca cctttggcga
    16621 tacagaggaa tttgtactcg tcgccatcaa cggcataaag atcgatctgc gctggcagat
    16681 tggtgccggt attcacctct ttatacatat ccagcggcgc gttttgcgag tagcgcaaat
    16741 tatcttcgat ataagtgtta tagacaccgc gcgccagcgc cgcttcatca cagccaccgg
    16801 tccatacacg ctgccctttt ttaccaacaa taatcgcagt gccggtatcc tgacaggttg
    16861 gcagaacacc tttcgccgcg atgtcggagt tgcgcaggaa ttgcagcgcc acatatttat
    16921 cattttcgct ggcttccggg tcacgcagaa tgtcggccac ctgttgttgg tgagccggac
    16981 gcagcataaa tgacgcatca tgaaacgcct gacgcgccaa cagagttaac gcttcgggtg
    17041 cgactttcaa aatctcctgc ccttcaaatt cagatacgct aacgtgttcg ctggttagca
    17101 ggtaatactc agtatcatct tttttgagtg gaaaaggagc ctgataatga aagggtttgt
    17161 ttgacattgt tctctcactt actgcctggt ttggttatgc tctgggcggg tgttccgttg
    17221 ccctgttaaa agcgagtaac aatatcctac acactttttt aacaaaaact gagactagta
    17281 cgactttttg cggctccagg ttacttcccg taggattctt gctttaatag tgggattaat
    17341 ttccacatta aaacagggat tgatcatgca aaaactcatt aactcagtgc aaaactatgc
    17401 ctggggcagc aaaacggcgt tgactgaact ttatggtatg gaaaatccgt ccagccagcc
    17461 gatggccgag ctgtggatgg gcgcacatcc gaaaagcagt tcacgagtgc agaatgccgc
    17521 cggagatatc gtttcactgc gtgatgtgat tgagagtgat aaatcgactc tgctcggaga
    17581 ggccgttgcc aaacgctttg gcgaactgcc tttcctgttc aaagtattat gcgcagcaca
    17641 gccgctctcc attcaggttc atccaaacaa acgcaattct gaaatcggtt ttgccaaaga
    17701 aaatgccgca ggtatcccga tggatgccgc cgagcgtaac tataaagatc ctaaccacaa
    17761 gccggagctg gtttttgcgc tgacgccttt ccttgcgatg aacgcgtttc gtgagttttc
    17821 cgagattgtc tccctactcc agccagtcgc aggtgcacat ccggcaattg ctcacttttt
    17881 acaacagcct aatgccgaac gtttaagcga actgttcgcc agcctgttga atatgcaggg
    17941 tgaagaaaaa tcccgcgcgc tggcgatttt aaaatcggcg ctcgacagtc agcagggtga
    18001 accgtggcaa acgattcgtt taatttctga attttacccg gaagacagtg gcctgttctc
    18061 cccgctgtta ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga
    18121 aacaccgcac gcttacctgc aaggcgtggc gctggaagtg atggcgaact ccgataacgt
    18181 gctgcgtgcg ggtctgacgc ctaagtacat tgatattccg gaactggttg ccaatgtgaa
    18241 attcgaagcc aaaccggcta accagttgtt gacccagccg gtgaaacaag gtgcagaact
    18301 ggacttcccg attccagtgg atgattttgc cttctcgctg catgacctta gtgataaaga
    18361 aaccaccatt agccagcaga gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt
    18421 gtggaaaggt tctcagcagt tacagcttaa accgggtgaa tcagcgttta ttgccgccaa
    18481 cgaatcaccg gtgactgtca aaggccacgg ccgtttagcg cgtgtttaca acaagctgta
    18541 agagcttact gaaaaaatta acatctcttg ctaagcttat taaaggctta taacaccttc
    18601 aggcggccag tccgcctgat ttcattttat ggataatcat tatgaataaa tcgctggtag
    18661 cggtaggcgt cattgttgcg ctaggcgtag tctggacagg cggcgcatgg tatacaggca
    18721 agaagattga aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga
    18781 cagctcctga atccaacctg gaagtgagtt atcaaaacta tcatcgcggc gtattcagca
    18841 gccagttgca actgttggtg aaacccattg ccgggaaaga aaatccgtgg attaaaagcg
    18901 gtcagagcgt catcttcaac gaatcggttg atcatggtcc cttcccgctt gcccagctta
    18961 aaaaactgaa cctgatcccg tcgatggcat caattcaaac cacgctggtt aataacgaag
    19021 taagcaaacc actgtttgat atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca
    19081 ttggttacag cggtgattcc agttccgata tttcgctcaa gccactgaat tacgagcaaa
    19141 aggatgaaaa agtcgccttt agcggcggcg agttccagtt aaatgctgac agagacggca
    19201 aagccatctc cctttccggg gaggcgcaaa gtggtcggat agacgcagtt aacgaataca
    19261 accagaaagt gcagttgacc tttaataatc tgaaaaccga cggttccagc acgctggcaa
    19321 gttttggtga gcgcgtagga aaccaaaaac tgtcactgga aaaaatgacc atttcagtgg
    19381 aaggcaaaga actggcactg ctggaaggca tggagatcag cggtaaatcg gatctggtca
    19441 atgacggtaa aacgatcaat agccaactgg attactcgct aaacagcctg aaggtacaga
    19501 atcaggatct gggcagcggc aagctgactt taaaagtcgg ccagattgat ggtgaagcct
    19561 ggcatcagtt tagccagcaa tataacgcgc aaactcaggc gctgctggca cagccagaaa
    19621 ttgccaacaa tcccgaactt tatcaggaga aagtgacgga agccttcttt agcgccctgc
    19681 cgctgatgtt gaaaggcgat ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc
    19741 agggtgaaag tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag
    19801 cgccgcaaac gctggcgcag gaagtagatc gttcggttaa atctctggat gcgaaactga
    19861 ccattccggt ggatatggca actgagttta tgactcaggt agcgaagctg gaaggttatc
    19921 aggaagatca agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca gcaatggggc
    19981 agatgttccg tctgaccacc ttgcaggaca ataccatcac caccagcctg caatatacta
    20041 acggtcagat aacgttaaac gggcagaaaa tgccactgga agatttcgtt ggtatgtttg
    20101 cgatgccagc tcttaacgtt ccggctgtac cggcaattcc gcagcagtaa ttcacggcca
    20161 aaacgccgga gaattttcac ttcggcgttt tcatctgaaa acaaaccaga gattgcctga
    20221 tgcgctacgc ttatcaggcc taccttttcc tgcaacactt tgaatttatg agtttttgta
    20281 ggctggataa ggcgtttaca ccgcatccgg cataaaaaac gcgcactttg tcaacaatct
    20341 gaaacgccgg aggtttttct ctccggcgtt atttctgact tcagcataaa gtcatactga
    20401 atactcattt tcacatccca gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt
    20461 tcccgccctt tatcggcagc aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc
    20521 cgatagttca taccacctaa gatacccgcg ccttcaatcc gcacatctcg tttactggat
    20581 tcatagccgc gagtcacaat ctgtgcaaat gtcagtgtaa attgcggcgt taaacgatag
    20641 ttgacgccca attgccagga ctgcattcct tcacggttac gatcaagact taatgaaaag
    20701 ggatagccaa tgtcggtatc caccacggca ccattcattg catggcgcaa ataaccacca
    20761 ctgccaaata cgcttcccca cggcgcattc caggtcaatt gtccactaac caacgccgtt
    20821 tcattgggct ggctggtatt acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac
    20881 tcccctttca acgtataatt cgggtcgagc ggtatgcttt tctcgagaat actttgtatc
    20941 tgattggaaa catctttctg taccgatgcc cccagagaat agcgactggt atcatcatgc
    21001 caactggcgg ctaaaaaaga acgtgcgcga tgtttatcgt attttcccgt ctggctgtta
    21061 tacacccctt catcacgaat accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc
    21121 cacattcccc acagatcaat atttggtgtt aaagcccaat aacctaatgc tccttcagaa
    21181 actgtcggta atgtacgttg cgaactgcca gccattaacg gaatacgata aggtaagcta
    21241 ccataactat aatccgtccc gaagcgacct gatttaagca ggctattttc actgggtttt
    21301 atttttactg cccccagggc aaagccaaaa cttttatcac catcaagata ccaccgggta
    21361 ctcatatcag cacgagcacc taatttataa acataatatg ccccgccttc aacgccaata
    21421 atattggaat aataaccact attgaaatcg agcaatcctc cctgtaccca tgcgtaaata
    21481 tctcttcccg cgccagcact gggcttatcc gccctgcgca attcgttttt cagtctgatg
    21541 cgtagtcccg cctcatcgtc agccagttgc gccgcataag cagagataag gccagaggca
    21601 gccgtcaggc aaataacggc catggccact atttttctca tagttgcacc tttgatgttc
    21661 tgattatttt tattgattat taattagtga tatcgctgat taattgctgc tgcacttttt
    21721 tacgattatc aatttcaacc acgatttctt tgaatttttt atccgtaagc ggataaaacc
    21781 agataataac gaatgccagt agcataaatc cgcaaggtac taaggcaatt gatgtgcgga
    21841 tgcccataat aacttccggc gtttgcacct gattggcaat atatccgctt aaccccaaaa
    21901 taaaggcagg aattgaacct ccgattgcct gaccgcattt acgggtaaat gagaatagtg
    21961 aataggtgag cccttcaatt cgcacgccgg tcaggtattc accgtattct acggtatcag
    22021 cttccagtgc ccacatcact gtcatagtaa cgccctgacc aattgaagcg atggccaacg
    22081 caaccaacgc caccggcagt gaccagacgg aaacccagaa gaatagcaga taaccgcagg
    22141 ttcccagcaa agcgccaatc aggaaggtat tctttttacc gatcctcgcg accatccccg
    22201 gcacaagcgg tgccgatgcc acagtaccaa ccaggttttg caccagtacc agcacagtga
    22261 acagcccggt atcatttaac acatagcgca cgtagaacaa cgacgaggcg ctgaccgcaa
    22321 aggtcgaaat cagcacacac agcgcaccga tgcacaacat aaacagcggg cgattccgtt
    22381 tcagggtttg caaactgatc ttcaatgacg gctgcgcaac gatacgtacc acattctcac
    22441 gcgtcgattt gaagcagatg aagtaaagca ccattccggc aatcgccagc acaatcgtcc
    22501 agaaatggta taccgacacc atctcttccg ggctggagtt cttaatgctc ggccctatca
    22561 gaaatgccag gcagacaaag gtcaatgaag cggcaatccc acgagccgcg cccagacggg
    22621 cgcgggattg tggttgttgg gtcatcgcgg tagcaagtga accataagga atattcacca
    22681 ggctgtagca aagcccgagg cccatgtagg tcaaatatgc atacactact ttgcggctat
    22741 ggctccagtc ggtcggtacc cagaatacca gcacgctgaa gatcattaac ggcgcagtac
    22801 cgaagagtaa aaacgggcgg aattttcccc agcgggtatt cacactgtcc accactcgtc
    22861 cggcaaagac gtcggcgaag gcatcgaata cccgcaccag taacagcatg gtgcccgccg
    22921 cagcggcacc gacgccagcg acgtcggtgt agtaactcaa caggaagagc acccccattg
    22981 cgaaggcgaa gttattggcg acgtcaccga ggctgtagcc gacgatggtg cgccaggaga
    23041 gttgttgatt cattgtttgc ctccctgctg cggtttttca ccgaagttca tgccagtcca
    23101 gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg tcgcgggtga agatcccctt
    23161 cttgttaccg ccaacgcgca atatgccttg cgaggtcgca aaatcggcga aattccatac
    23221 ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg tgatacatat ccagccatgc
    23281 acactgatac tcttcactcc acatgtcggt gtacattgag tgcagcccgg ctaacgtatc
    23341 cacgccgtat tcggtgatga taatcggctg atgcagtttc tcctgccagg ccagaagttc
    23401 tttttccagt accttctctg ccgtttccaa atcgccgctt tggacatacc atccgtaata
    23461 acggttcagg cacagcacat caaagagatc gctgatggta tcggtgtgag cgtcgcagaa
    23521 cattacattg acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag
    23581 tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc ggttcgttgg caatactcca
    23641 catcaccacg cttgggtggt ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg
    23701 cgcttgctga gtttccccgt tgactgcctc ttcgctgtac agttctttcg gcttgttgcc
    23761 cgcttcgaaa ccaatgccta aagagaggtt aaagccgaca gcagcagttt catcaatcac
    23821 cacgatgcca tgttcatctg cccagtcgag catctcttca gcgtaagggt aatgcgaggt
    23881 acggtaggag ttggccccaa tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt
    23941 atcgaatcct ttgccacgca agtccgcatc ttcatgacga ccaaagccag taaagtagaa
    24001 cggtttgtgg ttaatcagga actgttcgcc cttcactgcc actgaccgga tgccgacgcg
    24061 aagcgggtag atatcacact ctgtctggct tttggctgtg acgcacagtt catagagata
    24121 accttcaccc ggttgccaga ggtgcggatt caccacttgc aaagtcccgc tagtgccttg
    24181 tccagttgca accacctgtt gatccgcatc acgcagttca acgctgacat caccattggc
    24241 caccacctgc cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt
    24301 gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggatcccggc
    24361 atagttaaag aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac
    24421 cattcccggc gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac
    24481 acttttcccg gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg
    24541 atgctccatc acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa
    24601 acgcagcacg atacgctggc ctgcccaacc tttcggtata aagacttcgc gctgatacca
    24661 gacgttgccc gcataattac gaatatctgc atcggcgaac tgatcgttaa aactgcctgg
    24721 cacagcaatt gcccggcttt cttgtaacgc gctttcccac caacgctgat caattccaca
    24781 gttttcgcga tccagactga atgcccacag gccgtcgagt tttttgattt cacgggttgg
    24841 ggtttctaca ggacgtaaca taagggactc ctcattaaga taaaaatact ggtcaacctt
    24901 taatctgatt agttaaccaa ttctgtgttg cacacagtta tctgtgagaa acccatggaa
    24961 gagaaatgga aataaaaaga actgacaccg cgatcaaaaa accaaaaact gcaataccca
    25021 cagcgtgata ttgatcgcat taatctttaa aacggcgttg acagaagcca taaaataagc
    25081 aaccgaacgt tcgttcgagc atattgggaa cgataaactc tcatgacata tggctacggt
    25141 gaatattttt gggggggaat tctccgatcc cggctagatt gtattttgta cagggaaatt
    25201 aaagtatgaa acgcgggcac agttatcacc agcccgcgaa atgagaggat caggatgcgg
    25261 ttaagatacc gccaatcatc gcgcgtaatc cctgagcaaa cgacgcttca cgcgccagat
    25321 cttccgcttc gatatcggcc agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca
    25381 ttgacgtgtt gatgtctggg cttatctcgc ctttttgctg ggcatcacgc atccgctggg
    25441 caacaaattc cgtgatcgtc atatgcttat ttttaagcat ggcggcgacc tgtgggttac
    25501 gcatcccttc cgccataatt tcgaccacca gcgcccgttg cccaaaggct tcatgggtga
    25561 gagagacaat agactcgacc atatagtcaa caaaatgaat ccctttaatc ggttcccgga
    25621 aacgagccag cgccctctcc tggtcctgta agataatcgc ctgaatcaag gcttctttgg
    25681 agatgaaatg gtgatagagc gtcccgggac taatggcgca agatttacag atggctttca
    25741 tcgaggcact gtgaaatcca ttttctgaaa aaatctctct ggcagcattg aggatccggg
    25801 tccgtgtcgg ttgtgcttca gtctgcatgt tatccatcat taaaccatta tagttagcgt
    25861 gaataactag tttaccgcgt tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa
    25921 ggcacactct attcgtatac ttttcactct gaaattggtt aaccaatagc cgtgatttgg
    25981 ctgccagtta tttagcagag acgttgatga gtttatcccg gtaaacaaca cgcacggttg
    26041 tttaccggtc cgttagtgta ttaattgagc tcctgtaccc caccaccgga gacggtgaga
    26101 atttgtccgc ttacccagct cgcagcaggc gagcaaagga acagcgctgc gttagcaata
    26161 tcttgcggtt ggcccagacg tctgatcggc gtgtgctgta acattttttg ttcaatttct
    26221 ggtgtaataa cggatttcag ggcatcggtt aatattgccc ccggcgcaat gccatttacc
    26281 cgaatatttt tttcacccag gtcaaacgcc atatttctga ccagatgact ggctgcagct
    26341 ttagatgatg cataggaagt catgtttata tttttatttt ctgccgccat agaagtgatg
    26401 gtcagaataa cgccaccgcc atttttttcc atttctggcg caacaagttg tgacagatgg
    26461 aaaaaagaaa acacattcag ttcataagca cggcgaaaat ccgccattgg catatcaaac
    26521 ggtttaggtc caccgccacc ggcgttgtta accagaatat caactttacc cagcttactg
    26581 atagcaaagt ctgccagtgc agagagttcc tgttcggaag taatatcaca acggcaggca
    26641 aatgcctgac cacccagttg ttgaatttcg tctacaacat ggttagctgc gtcggcgtta
    26701 atatcactga ccaccacaga tgcgccagct gtcgcgaatg taatggcgat ttctttacca
    26761 atacctgcac ccgcacctgt gatgatggcg catggcgcat ggcgcatggc gcatggcgca
    26821 tggcgcatgg cgcatggcgc atggcgcatg gcgcatggcg catggcgcat ggcgcatttt
    26881 ccgtcga
//
LOCUS       DAJTYZ010000049        26827 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000049 DAJTYZ010000000
VERSION     DAJTYZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.049"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..924
                     /locus_tag="OO596_001957"
     CDS             <1..924
                     /locus_tag="OO596_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6717820.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="OO596_001958"
     CDS             complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="OO596_001958"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6717821.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1891..3927
                     /gene="lpoA"
                     /locus_tag="OO596_001959"
     CDS             1891..3927
                     /gene="lpoA"
                     /locus_tag="OO596_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6717822.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3885..4280
                     /locus_tag="OO596_001960"
     CDS             3885..4280
                     /locus_tag="OO596_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6717823.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4300..4890
                     /gene="diaA"
                     /locus_tag="OO596_001961"
     CDS             4300..4890
                     /gene="diaA"
                     /locus_tag="OO596_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6717824.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4900..5475
                     /gene="yraP"
                     /locus_tag="OO596_001962"
     CDS             4900..5475
                     /gene="yraP"
                     /locus_tag="OO596_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6717825.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5589..6629)
                     /locus_tag="OO596_001963"
     CDS             complement(5589..6629)
                     /locus_tag="OO596_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6717826.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6702..7337)
                     /locus_tag="OO596_001964"
     CDS             complement(6702..7337)
                     /locus_tag="OO596_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6717827.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7465..7983
                     /gene="yhbO"
                     /locus_tag="OO596_001965"
     CDS             7465..7983
                     /gene="yhbO"
                     /locus_tag="OO596_001965"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6717828.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7963..8406)
                     /locus_tag="OO596_001966"
     CDS             complement(7963..8406)
                     /locus_tag="OO596_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6717829.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8457..8759
                     /gene="yhbQ"
                     /locus_tag="OO596_001967"
     CDS             8457..8759
                     /gene="yhbQ"
                     /locus_tag="OO596_001967"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6717830.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8746..9249)
                     /locus_tag="OO596_001968"
     CDS             complement(8746..9249)
                     /locus_tag="OO596_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6717831.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9243..9767)
                     /locus_tag="OO596_001969"
     CDS             complement(9243..9767)
                     /locus_tag="OO596_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6717832.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9976..10971
                     /locus_tag="OO596_001970"
     CDS             9976..10971
                     /locus_tag="OO596_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6717833.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10980..11858
                     /locus_tag="OO596_001971"
     CDS             10980..11858
                     /locus_tag="OO596_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6717834.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12064..13071
                     /locus_tag="OO596_001972"
     CDS             12064..13071
                     /locus_tag="OO596_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR6717835.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="OO596_001973"
     CDS             complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="OO596_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6717836.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="OO596_001974"
     CDS             complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="OO596_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6717837.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="OO596_001975"
     CDS             complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="OO596_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6717838.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="OO596_001976"
     CDS             complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="OO596_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6717839.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="OO596_001977"
     CDS             complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="OO596_001977"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6717840.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="OO596_001978"
     CDS             complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="OO596_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6717841.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20449..21393)
                     /gene="truB"
                     /locus_tag="OO596_001979"
     CDS             complement(20449..21393)
                     /gene="truB"
                     /locus_tag="OO596_001979"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6717842.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="OO596_001980"
     CDS             complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="OO596_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6717843.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21958..24630)
                     /gene="infB"
                     /locus_tag="OO596_001981"
     CDS             complement(21958..24630)
                     /gene="infB"
                     /locus_tag="OO596_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6717844.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="OO596_001982"
     CDS             complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="OO596_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6717845.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="OO596_001983"
     CDS             complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="OO596_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6717846.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         6356 a         7449 c         6746 g         6276 t
ORIGIN      
        1 acctctgcaa cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag
       61 gcagttaaac ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga
      121 tattacaaac tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca
      181 ggactcacaa ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc
      241 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
      301 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
      361 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg
      421 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
      481 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
      541 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
      601 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      661 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      721 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      781 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      841 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
      901 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      961 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
     1021 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     1081 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     1141 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     1201 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     1261 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     1321 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     1381 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     1441 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     1501 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1561 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1621 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1681 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1741 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1801 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1861 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1921 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     1981 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     2041 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     2101 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     2161 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     2221 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     2281 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     2341 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     2401 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     2461 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     2521 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     2581 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2641 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2701 gtatttggtc gcaccattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2761 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     2821 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     2881 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     2941 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     3001 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     3061 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagcca
     3121 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     3181 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     3241 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     3301 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     3361 ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc
     3421 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     3481 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     3541 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caatgctgta cgccagctcc
     3601 cgcagcgcac aaggcaccgc aggcccggat ttccgtctgg agatggaagg tttgcagtac
     3661 agcgaaatcc cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg
     3721 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     3781 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     3841 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     3901 caaggtcagg tagtccccgt cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     3961 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     4021 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     4081 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     4141 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     4201 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     4261 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     4321 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     4381 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     4441 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     4501 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     4561 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     4621 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     4681 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     4741 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     4801 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     4861 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     4921 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     4981 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     5041 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     5101 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     5161 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     5221 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     5281 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     5341 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     5401 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     5461 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     5521 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     5581 ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc
     5641 gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     5701 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     5761 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     5821 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     5881 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
     5941 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     6001 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
     6061 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     6121 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     6181 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     6241 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     6301 cgaaagcgcg attgtccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     6361 aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc
     6421 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     6481 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     6541 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     6601 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     6661 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     6721 aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     6781 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggcag taaacgaaac
     6841 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag caacatcgac
     6901 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     6961 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     7021 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     7081 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     7141 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     7201 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
     7261 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     7321 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
     7381 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgtgaaa ttcgcctcat
     7441 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cagtgatgaa
     7501 tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     7561 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     7621 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
     7681 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     7741 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc
     7801 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     7861 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     7921 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     7981 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat
     8041 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     8101 attgtacgcc tggcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     8161 aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     8221 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     8281 ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccacagtt ccccttcctg
     8341 ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg tgatgagtgt
     8401 ttccattgct gctctctttt atactgtggg cctgacctta acatgcgacg cagacgatga
     8461 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     8521 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     8581 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac
     8641 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     8701 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
     8761 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     8821 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     8881 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     8941 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     9001 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     9061 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     9121 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     9181 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     9241 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     9301 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     9361 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
     9421 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     9481 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
     9541 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     9601 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
     9661 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     9721 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     9781 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     9841 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     9901 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gaaagcgcaa
     9961 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    10021 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac taatgcccgt
    10081 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    10141 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    10201 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    10261 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca
    10321 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgatgtt
    10381 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtc
    10441 acacctgtcc cgctggaagt ctttgctttt ggcagcctgt gcattatgtc ggaaggtcgt
    10501 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg
    10561 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    10621 ctgatcgacc gttatcagga tggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
    10681 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    10741 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    10801 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    10861 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
    10921 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    10981 tgaaatattc cttagggcca gtgctgtggt actggccaaa agaggcgctg gaagaatttt
    11041 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    11101 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagcg
    11161 ggaagcagat agtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    11221 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    11281 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    11341 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    11401 tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagttgggg attcgcaacc
    11461 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    11521 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    11581 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    11641 agaccatgag cggctacgtt tacaacctcg gtaacgagct gacatccatg cagggattgg
    11701 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    11761 gcgctaatga aaatggcgct gcgccactgc cgttgacggc aaacagcgac tgtaacggct
    11821 actggcggcg gctggctggg ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    11881 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    11941 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    12001 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    12061 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    12121 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    12181 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    12241 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    12301 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    12361 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    12421 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    12481 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    12541 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    12601 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    12661 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    12721 gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctattt
    12781 acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccggaca actgccgccg
    12841 ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt gcagcaggcg
    12901 ctgagtatgt cgttggtggg tgataaagcg aaagtgcgtc acggcttgca gtcgatcctg
    12961 cgcgaaaccg acgccgatga aattatggtt aacgggcaga ttttcgacca ccaggcgcgc
    13021 ctgcattcgt ttgaactggc gatggatgtg aaggaagagt tgttgggata gtgtgtctta
    13081 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    13141 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    13201 cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaacaccag
    13261 aatcagcgta atcatcggct tgccgcccca gacacggaat ttcgggctgc caaagcgttt
    13321 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    13381 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    13441 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    13501 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    13561 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    13621 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    13681 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    13741 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg
    13801 cacgttaccg tgataaccaa acgatgccag acagaacggc agggtcatca gcaaatacgg
    13861 cgcataagat gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag
    13921 taggctaccg aaggtgagga agaaggtaat gactttcgcc cccagtacaa tcgccgtcat
    13981 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    14041 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    14101 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc
    14161 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc
    14221 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    14281 ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag
    14341 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    14401 cggtgacgtt tgggtggtgg ttagtgttgc catgagagct tctctccagt gaaaaatagt
    14461 gcgactgcgt tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    14521 gccccgatgg taaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag
    14581 cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc
    14641 gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacggccac cttcacggcg
    14701 ttcaccgctg aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc
    14761 acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt
    14821 gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac ctttcggcag
    14881 ttcgatggtg gagtgagaag caaacagctt gatgttacca atgtaacggc tactgatgtc
    14941 gccttcgtta gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac
    15001 ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg gacgatcttc
    15061 acggtcacca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg
    15121 gaattcacgt ttcggacgca tcggcgcatc tggcggtacg atcagagtac gttcaccctg
    15181 tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac cttctgcagt
    15241 cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg
    15301 ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag
    15361 ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc
    15421 gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat
    15481 acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca ggctgatacg
    15541 ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc
    15601 tttcaggcgt tccagtgtct gttcacgcag cgcctggttc atgtcaccgt tcagcgcggc
    15661 gctgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt ttttggtacg
    15721 aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt
    15781 tttgcgcata ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct
    15841 ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc
    15901 ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat
    15961 ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag
    16021 accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg
    16081 agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg
    16141 gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc
    16201 ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt
    16261 cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc
    16321 cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg
    16381 aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag
    16441 gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa
    16501 tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg
    16561 aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc
    16621 aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg
    16681 tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag
    16741 ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc
    16801 agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag
    16861 gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc
    16921 aggtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt
    16981 ttcaacactt ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga
    17041 ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac
    17101 ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt
    17161 ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc
    17221 gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct
    17281 tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc
    17341 tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc
    17401 atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca
    17461 cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac
    17521 cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt
    17581 cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa
    17641 tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac
    17701 tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac ttctagaact
    17761 ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca
    17821 gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca
    17881 aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg
    17941 atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc tgcgatcttc
    18001 acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag ggcacggata
    18061 acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt gatggtatgg
    18121 atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg
    18181 cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg
    18241 gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacctgca
    18301 actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg
    18361 tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc
    18421 acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaagaaccg
    18481 ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc catatccggc
    18541 atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga
    18601 gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag
    18661 gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc
    18721 gttgcagtaa ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc gtgagtacgc
    18781 ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata
    18841 cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt
    18901 tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca
    18961 gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatca
    19021 ctcagacgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc
    19081 ggctgccagt cccaacgcgg tttaccggct tctttcacta gttcattgat gttctgaata
    19141 acaacctgct gttgttcatg accgaacact actgcgccca gcatctgatc ttcgctcagc
    19201 agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac aaccagatcc
    19261 agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa
    19321 cctacgcggg cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg
    19381 gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg
    19441 gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca
    19501 atcagacgcg cgatcagggt ttcgccttcg cttggacgac cttcacgacg gaagaagcta
    19561 cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag
    19621 tcctgacctg gtttggcttt tttctgtcca acaacggtaa cgaataccgc ggtgtcatcc
    19681 atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg
    19741 gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc
    19801 tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg
    19861 actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc
    19921 tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc
    19981 agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc
    20041 agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg
    20101 agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt
    20161 ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga
    20221 acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt
    20281 gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc
    20341 tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa
    20401 gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta
    20461 ttcaaccacc aggcgacgag gcgcgacgcg gccttcatcg tcaatttcgc ccataccgat
    20521 aaacttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt
    20581 acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg
    20641 gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg
    20701 aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg
    20761 ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc
    20821 gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat
    20881 ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg
    20941 gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg
    21001 atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag
    21061 cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc
    21121 ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga
    21181 gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgcctgt
    21241 tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac
    21301 tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt
    21361 aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta
    21421 acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct
    21481 tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct
    21541 ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta
    21601 accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg
    21661 cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca
    21721 cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca
    21781 aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa
    21841 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    21901 ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta
    21961 agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac
    22021 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    22081 atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag
    22141 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatagc
    22201 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    22261 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat
    22321 gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc
    22381 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt
    22441 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    22501 tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat
    22561 gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    22621 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg caacttcacg
    22681 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag
    22741 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    22801 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    22861 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    22921 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    22981 cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag atacgtgtac
    23041 gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt
    23101 aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca ccaccggtac
    23161 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc
    23221 agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    23281 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    23341 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg ttgaacgaat
    23401 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc
    23461 gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    23521 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    23581 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    23641 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac
    23701 aacgtcacgg ttaacggcct gagctggctt ctggaagcct tgctgcaggg aagaaccttt
    23761 acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    23821 ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    23881 accgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt
    23941 gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    24001 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgatgcgc
    24061 ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    24121 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    24181 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcagcctcac gttcagcttt
    24241 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    24301 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    24361 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    24421 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    24481 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    24541 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    24601 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    24661 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ccttttcgtc
    24721 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    24781 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    24841 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    24901 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    24961 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    25021 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    25081 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    25141 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    25201 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    25261 agcaacgtct gccggtgcca ttgcgttaat cacgaactgc gccgggttat catcccacag
    25321 gacgatatcg atacgctcgc cacccagttc agtagacacc gcctgaacac gcgcgccacg
    25381 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    25441 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    25501 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    25561 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    25621 gcggaagttt tcacgcggca gcatatcttc acgcaggatc acggcttcag cgttgttgcc
    25681 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    25741 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    25801 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    25861 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    25921 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    25981 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    26041 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    26101 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    26161 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    26221 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    26281 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    26341 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    26401 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    26461 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    26521 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    26581 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    26641 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    26701 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    26761 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    26821 agggaaa
//
LOCUS       DAJTYZ010000050        25773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000050 DAJTYZ010000000
VERSION     DAJTYZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.050"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..827)
                     /gene="dgt"
                     /locus_tag="OO596_001984"
     CDS             complement(<1..827)
                     /gene="dgt"
                     /locus_tag="OO596_001984"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6717847.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
     gene            911..1609
                     /gene="mtnN"
                     /locus_tag="OO596_001985"
     CDS             911..1609
                     /gene="mtnN"
                     /locus_tag="OO596_001985"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6717848.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1602..2402
                     /gene="btuF"
                     /locus_tag="OO596_001986"
     CDS             1602..2402
                     /gene="btuF"
                     /locus_tag="OO596_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6717849.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2440..3063
                     /locus_tag="OO596_001987"
     CDS             2440..3063
                     /locus_tag="OO596_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6717850.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="OO596_001988"
     CDS             complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="OO596_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6717851.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3447..3512)
                     /locus_tag="OO596_001989"
     CDS             complement(3447..3512)
                     /locus_tag="OO596_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717852.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="OO596_001990"
     CDS             complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="OO596_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6717853.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5182..6462
                     /gene="hemL"
                     /locus_tag="OO596_001991"
     CDS             5182..6462
                     /gene="hemL"
                     /locus_tag="OO596_001991"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6717854.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="OO596_001992"
     CDS             complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="OO596_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6717855.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="OO596_001993"
     CDS             complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="OO596_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6717856.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="OO596_001994"
     CDS             complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="OO596_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6717857.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="OO596_001995"
     CDS             complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="OO596_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6717858.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="OO596_001996"
     CDS             complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="OO596_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6717859.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="OO596_001997"
     CDS             complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="OO596_001997"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6717860.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17979..18509
                     /gene="thpR"
                     /locus_tag="OO596_001998"
     CDS             17979..18509
                     /gene="thpR"
                     /locus_tag="OO596_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6717861.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18524..19228
                     /gene="sfsA"
                     /locus_tag="OO596_001999"
     CDS             18524..19228
                     /gene="sfsA"
                     /locus_tag="OO596_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6717862.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19406..19861
                     /gene="dksA"
                     /locus_tag="OO596_002000"
     CDS             19406..19861
                     /gene="dksA"
                     /locus_tag="OO596_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6717863.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19898..20824
                     /gene="gluQRS"
                     /locus_tag="OO596_002001"
     CDS             19898..20824
                     /gene="gluQRS"
                     /locus_tag="OO596_002001"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6717864.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20863..22281
                     /gene="pcnB"
                     /locus_tag="OO596_002002"
     CDS             20863..22281
                     /gene="pcnB"
                     /locus_tag="OO596_002002"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6717865.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            22278..22757
                     /gene="folK"
                     /locus_tag="OO596_002003"
     CDS             22278..22757
                     /gene="folK"
                     /locus_tag="OO596_002003"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6717866.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            23127..23711
                     /locus_tag="OO596_002004"
     CDS             23127..23711
                     /locus_tag="OO596_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6717867.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23816..24556
                     /locus_tag="OO596_002005"
     CDS             23816..24556
                     /locus_tag="OO596_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6717868.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24591..25773
                     /gene="htrE"
                     /locus_tag="OO596_002006"
                     /pseudo
     CDS             24591..25773
                     /gene="htrE"
                     /locus_tag="OO596_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6330 a         6984 c         6416 g         6043 t
ORIGIN      
        1 tctgccacac aataggagat gtcgtcggca gcttccataa tccacgttaa tggaaaacga
       61 ctgtaaagcg ccaaattaag ttctttacgc aaccgggcaa tataggcttc ttcagaaaga
      121 taataacccg gctttttcat taaatagtga tgtgtctcag gcgtttcgcc acgccaccac
      181 gccggacggg tatattttaa aataccgcca acctgtgccc aagtgagatt catccgcatc
      241 aatgtatgca ccagacgaat gccttgtgca ttcccctcaa aatgacataa gtcctgacga
      301 atcttgcgcc gcagctcgtt aagctgttct tccccgtccc gtaaacgtag tgccgccacg
      361 ctgcagcgat cgtcagtcag aggctggctt tcggcatctt ccgggtgcaa acgttggcga
      421 aaccagtcat ttatcgctgc ttcgccaaaa tgaccaaacg gcggattgcc gatatcgtgc
      481 atcaggcatg acatctcaac aatgctttca aaggggccgg tcagttcatc caggccgtat
      541 gcttccagca atttaagctc tttcagacgg cttaaaattt ctttggcgat gtagcgtccc
      601 acctgctgga cttccatcga gtgggtaaga cgcgtgcgca cggcggcatt gcgctccagt
      661 ggaaaaacct gggtcttttg ttgcagacga cgaattgccg gagagttgat gatacgcccg
      721 cgatcgctct cgaagatccg caggatctca tgttcggttt taacgccctg cggtgaacgg
      781 taacgacgat gccagtttat tttttttcgg aaatcaatct gtgccatgtc ctctcccgcg
      841 tgagaaatac gcttccccgt aagcgcatgg taaactatgc tttcaaatcg ggcttatcgc
      901 gagtaaatct atgaaaatcg gcatcattgg tgcaatggaa gaagaagtta cgctgctgcg
      961 tgacaaaatc gaaaaccgtc aaactatcag tctcggcggt tgcgaaatct ataccggcca
     1021 actgaatgga accgaggttg cgcttctgaa atcgggcatc ggtaaagtcg ctgcggcgct
     1081 gggtgccact ctgctgttgg aacactgcaa gccagatgtg attattaaca ccggttctgc
     1141 cggtggcctg gcaccaacgc tgaaagtggg cgatatcgtt gtctcggacg aagcgcgtta
     1201 tcacgacgcg gatgtcacgg catttggtta tgaatacggt cagttaccag gctgtccggc
     1261 aggctttaaa gctgacgata aactgatcgc tgccgctgag gcctgcattg ccgaactgaa
     1321 tcttaacgct gtacgtggcc tgattgttag cggcgacgct ttcatcaacg gttctgttgg
     1381 tctggcgaaa atccgccaca acttcccaca ggccattgct gtagagatgg aagcgacggc
     1441 aatcgcccat gtctgccaca atttcaacgt cccgtttgtt gtcgtacgcg ccatctccga
     1501 cgtggccgat caacagtctc atcttagctt cgatgagttc ctggctgttg ccgctaaaca
     1561 gtccagcctg atggttgagt cactggtgca gaaactggca catggctaag tcactgttca
     1621 gggcgctggt cgccctgtct tttcttgcgc cactgtggct caacgccgcg ccgcgcgtca
     1681 tcacgctttc tcccgccaac actgaacttg cctttgccgc cgggatcacg ccggttgggg
     1741 tcagcagcta ttccgactat cctccacaag cgcaaaagat tgagcaggtt tccacctggc
     1801 aggggatgaa tctggaacgc attgtcgcgc tgaaacccga tctggtgatt gcctggcgtg
     1861 gaggtaatgc cgagcggcag gttgaccagc tggcttcgct gggaataaaa gtgatgtggg
     1921 tcgatgcgac aagcattgaa caaattgcca atgcgttacg tcaattggcc ccctggagtc
     1981 cgcaaccgga taaggccgaa caagccgcgc aatccctgct ggatcagtac gcgcaattga
     2041 aagcgcaata tgctgataaa cctaaaaaac gtgtttttct gcaattcggc attaatccgc
     2101 catttaccag tggaaaagag tcgattcaaa accaggtact cgaagtttgt ggcggagaaa
     2161 acatctttaa agacagccgg gttccctggc cgcaagttag ccgcgaacag gtgttagcac
     2221 gctcgccaca ggcgattgtc attacaggcg gaccggacca aattcctaaa atcaaacaat
     2281 actggggtga acagctcaaa attcccgtta ttcctctcac gagtgactgg tttgaacgtg
     2341 caagcccacg tattatcctc gctgcacaac agctctgtaa tgcgctttca caggtagatt
     2401 agcgcctgac ctttttaggt gctcagtggg gattgaacaa tgctcgtcta ttggctggat
     2461 atagtcggca cagcggtatt tgccatctcc ggcgttttgt tagccggaaa attgcgtatg
     2521 gacccttttg gtgttctggt actgggcgtg gttaccgctg taggcggcgg gacaattcgc
     2581 gacatggcgc tggatcacgg cccggtattt tgggtgaaag atcccaccga tctggtcgtt
     2641 gcaatggtaa ccagcatgct gaccatcgtg ctggtgcgcc agccaagacg cttaccaaaa
     2701 tggatgttgc cggtgctgga cgccgttggt ctggcggtgt ttgtcggcat tggcgtgaat
     2761 aaagccttta atgcggaagt cggtccgtta atcgcggttt gtatggtcgt cattactggc
     2821 gttggcggcg ggatcattcg tgatgtactg gcccgcgaga tccccatgat tttacgtaca
     2881 gaaatctacg caactgcctg tattatcggc ggtattgtcc acgctacggc ttattacaca
     2941 ttttccgtac cactggaaac agccagtatg atgggcatgg tcgtgacgct attgattcgg
     3001 ctggcggcta ttcgttggca tcttaagcta ccgacgtttg cgctggatga gaatgggcgt
     3061 tgaagagaga agatgcgccg gatagtttat ccggcacatg aacaacagat tagatgctaa
     3121 aggaagaacc gcaaccgcag gtgcttttcg cgttcgggtt ggtcacgatg aaacgagaac
     3181 cttccagtcc ttcggtatag tcaacggaac cgccgaccag atattgcagg ctcatcggat
     3241 caaccaccaa gccaacgccc tgtttttcga tggtcatatc gccttcgttc acctgatcat
     3301 caaaggtgaa accatactgg aagccgctgc aaccgccacc ggtgatatac acgcgtaatt
     3361 tcagattcgg gttatcttcg tcagcgatca ggcttttaac tttgttggct gctgcgtcgg
     3421 taaactccag cggcagtgct acgtcatcac tcatattttg ctccaaacga catcggcaat
     3481 tgggcaaatt ctaacccaat aataatcgcc attatctaat accctggtat ttcgttcaag
     3541 tattctcgct ggctgatgcg gccttgcttc gcgccagttg ctcagcttcc tgttttgcca
     3601 gcgtgcgcgc aagaatcgcc gagtatagcg gtttcccgcc ggtaaattgc gctaatagtg
     3661 ttgcgccaag accggtaata atcattggca aaatgagctg gtagttatcg gtcatctcca
     3721 gaaccagaat gatccccgtt aacggcgcgc gaatagatgc cgccagtaat gcccccattc
     3781 cggcaatagc aaacgtcccc gcctcaaggt gatattgcgg aaacagctca acggcaacca
     3841 ttccgaaagc ggttcccagc acagtaccca gcgccagcat cggggcaaaa ataccgcccg
     3901 gcgcgccgga agagaagcag agtaaggtgg taatgacccg agcgacgaag ataaacacca
     3961 gcattcccat gctgaaattc cccgcggtag cgataggaat caggttaaaa ccgccgcccg
     4021 acgttgctgg tgccacaaac cccagcaatc cacacagacc gccaatcgca ccgcccatca
     4081 gtacccattt ggtaatattg ccgccgtgca cacggtgcag caaatcctgc atccccagca
     4141 cccatttatt aaaaataggg ccgaaaatgc caaaaataat accgaggatc agataaagcc
     4201 acagcgtatt aagcggcgca tcagaaagtt taccgacgtc aatcaacgca acttcatgat
     4261 taaaaatccg gtacataatg gtcgacataa tgacgccaat aaataccgct tttatcgaaa
     4321 ttaacgtata gcgaaactgc ggacgcatct cttcgataat aaacaaaata cccgccagcg
     4381 gcgcgttaaa ggccgcagcc agccccgcag cagcaccggt tgccagcagc gtatggcgag
     4441 cttcgtcacc tttcaggcgg aaaatatcaa gcaccatacg gccaatgtta ccgccgatct
     4501 gcacggttgg cccttcgcgc cccaacacca tgcctccgcc gagtgtcccc agcccgccaa
     4561 agaacttcac cggcaataca cgccaccagc gaacgggacg ttgatcttcc agcgcccctt
     4621 caatttccgg gatccccgaa ccacctgctt ccggcgcgta tttgcgcacc aaaaagtagc
     4681 caaacatcgc cagcaccgcc gaacagagaa aagcgacggt taacagaagc ggataattat
     4741 cagcagtatg taccagcgcc cccatacgtt ggttctgcaa ccaggcgaca cctttgtcaa
     4801 aagcaaccgc tgccagccca acaagcgtgc cgacgactgc cgccataaac aaaatggcta
     4861 acggggtttt atcgcgctca agaagttggc gaatcagttg tctgcgtcgc aggcgcgcgg
     4921 cctgcggtgt ttctaaagag ggagtatcag ttttcatcca atgatcactt attggtcata
     4981 caaataagat gacggcattt tactcgcacg ttccatgaaa atcctttgca aagcgtaatg
     5041 tttcaaatgc acaaaacttt tatactctaa gaaatcagga ttgcaccagt acaagcagcc
     5101 tgatgtttga cgagtattta acttgttatg aataacatag aatagcagcc attcactttt
     5161 tctacgaatc aggaaccctc catgagtaag tctgaaaatc tttacagcgc agcgcgcgag
     5221 ctgatccctg gcggtgtgaa ctcccctgtt cgcgccttta ctggcgtggg cggcactcca
     5281 ctgtttatcg aaaaagcgga cggcgcttat ctgtacgatg ttgatggcaa agcctatatc
     5341 gattatgtcg gttcctgggg gccgatggtg ctgggccata accatccggc aatccgcaat
     5401 gccgtgattg aagccgccga gcgtggttta agctttggtg caccaaccga aatggaagtg
     5461 aaaatggcgc aactggtgac cgaattggtc ccgaccatgg atatggtgcg catggtgaac
     5521 tccggcaccg aggcgacgat gagcgccatc cgtctggccc gtggttttac cggtcgcgac
     5581 aaaatcatta aatttgaagg gtgttaccac ggtcacgctg actgcctgct ggtgaaagcc
     5641 ggttctggcg cactcacgtt aggccagcca aactcgccgg gcgttccggc agatttcgcc
     5701 aaacatacct taacctgtac ttataacgat ctggcttctg tacgcgccgc gtttgagcaa
     5761 tacccgcaag agattgcctg tattatcatc gagccggtgg caggcaatat gaactgtgtt
     5821 ccaccgctgc cagacttcct gccaggtctg cgtgcgctgt gcgacgaatt tggcgcattg
     5881 ctgatcatcg atgaagtaat gaccggcttc cgcgtggcac tggctggcgc acaggattat
     5941 tacggtgtgg aaccggatct cacctgcctg ggcaaaatca tcggcggtgg aatgccggta
     6001 ggcgcattcg gtggtcgtcg tgatgtaatg gatgcgctgg ccccgacggg tccggtctat
     6061 caggcgggta cgctttccgg taacccaatt gcgatggcag ccggtttcgc ctgtctgaat
     6121 gaagtcgcgc aaccgggcgt tcacgaaacg ttggatgagc tgacaacacg tctggcagaa
     6181 ggtctgctgg aagcggcaga agaagccgga attccgctgg tcgttaacca cgttggcggc
     6241 atgttcggta ttttctttac cgacgccgag tccgtgacgt gctatcagga tgtgatggcc
     6301 tgtgacgtgg aacgctttaa gcgtttcttc catatgatgc tggacgaagg tgtttacctg
     6361 gcaccgtcag cgtttgaagc gggctttatg tccgtggcgc acagcatgga agatatcaat
     6421 aacaccatcg atgctgcacg tcgggtgttt gcgaagttgt gatccagcat ggtttgtcgg
     6481 atgcggcgtg aacgccttat ccgacctacg attcggggcc gtttgtaggc ccgataagac
     6541 gcgccagcgt cgcatcgggc atccgttgtc gcgtaaacgc tttatccgtc caggcgtaca
     6601 gggccgttat atggaaaaat taacggctct gctttctcag caaatagata aaatatggcg
     6661 cgccgataaa ggtcgacagc agccccgccg ggatctggaa tggaaacagc accatccgcc
     6721 cacaccagtc agcgaatacc agcagtaaac cacccaccag cgccgaaatt acgatgtgtg
     6781 gcatcgtccg tcgaaagccc atcatccgcg caatatgcgg tgccattaaa ccaacaaaac
     6841 tcaacggtcc aatagtcatc gtcgcggtcg ccgtcaggca agccgctaac agcagcagcg
     6901 caattcgcgt cggcgtcagc gccattccta cggctcgtgc ggtatcacca cccagcggta
     6961 aaatggtcag ccagcggcgg tacagcgggg taatcgccag caaaatcacc atcacaattc
     7021 cggtacgcca gacctgcgca tcggtcgcgt tgtaggtcga accggaaatc caggtcagca
     7081 cttgcgccat tcgcgggtca ccgctcgcct gcaacatcat caaaagcatg gtgaatgcgg
     7141 tacttaacgc catccccgcc agtaacatac ggtgtgggga aaatccaccg cggccggcgg
     7201 cgatcataat gatcaacagc gtcaccgccg ccccgatact gcccgcgggt aacagccagc
     7261 caaaggcatt acccggcacc agaaacagca ttaacaccac gccaaacgcc gcgccagagc
     7321 taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga ataatacagc
     7381 ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc cagcgccagg
     7441 gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca tcacgaccaa
     7501 acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg gcaaacgcca
     7561 gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc gcgctaatgc
     7621 tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca gtgaccgaac
     7681 cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg gaaagccaga
     7741 ggatcagcgc cccaatcagc gacgccagca tcaaccgtgg caacaaacgc cgcgccccca
     7801 gcattttcgc cagcagcggc gcgaacaaac cgataaagcc gataatcccc accgcattca
     7861 ccagcagcgc actgatgact atcgccagcg acagcgccgc cagacgcgcg agcgacaagg
     7921 ccagcccgag attgcgcgcc acgccatcat caagccccat cagggttaac gggcgaagta
     7981 gcaataacgt caacatcacg ccgcccagca actgcggcca taaacgctca acgccgcccc
     8041 agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagc tggtcgtgat
     8101 ggaagataac cagtagctga ttaattgccc cgcaataaag gctcaccacc agccccgcga
     8161 gaataagcgt aaccggcgaa agccgtttcc cccacgcgac gccaaagacg attaagccaa
     8221 cgacacaagc gcccaccagt gcggcaaact ggctcgccat agcacctggg atcgcccaaa
     8281 gtgtggtgac ggtaattccc agttgcgcgc ctgtagcaac gccaagcgtc gtcggctccg
     8341 ccagcgggtt acgcagcact tgctgaaaca gcacgcccac cagccccagg cccgcgccca
     8401 ccagcagcga aatcgccaga cgcggcaaca agctgtagtg aaaaatcatc tgctcgatga
     8461 catcgatgtc tggcgaccag gcagcctgcg cccactggct acgcggcagc gcctgcgaga
     8521 agttcatcca ggtgagcgcc gtagcgacaa tcactaacag cgccagcaat aacgccggaa
     8581 aaagcgcaat tcgtttactc acgctttacc tccgatggcg ttatccagaa cgcgcacaaa
     8641 gtgcattgcc gagagtgtcg caccataaaa ccagactgca ggtacgcgct gaaagcgtcc
     8701 ggcgcggaca aacggcatgg cctgccacag cggggttgcc attagcgcat ccatgtcttt
     8761 gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca gacgatcgat
     8821 actgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg ggatgccgta
     8881 ctcatcgaga atttcctgga acaagctgtt tggaccgaag accagcatat ggcgcggatc
     8941 gataagcgtc gtcagcagta acgggcgtgc accacgcttc acaaagcggg gtttcatgct
     9001 gcggataaag tcttcgtatt gcgctaaatg cgtttccgct gcgctttgca ggttaagtaa
     9061 atctgccatt tccgtcagcg atttacgcgc catcgccaac ggctgtttgc cgtcactgaa
     9121 gttaaatccg cgacccggcg caatacgtgc cagcatttct gatgaagggc catatcctgc
     9181 cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcaaggttag gttctgtacg
     9241 caaaccgacg tcaatcactg agtccggcaa tggtggttcg ctgacccaca ggcgatagtt
     9301 gatggtatcc gccacgccgt aaggcacgat gccgagcgcc agcagtaatt ccaccggcaa
     9361 ccactccagc gccacaatac gattggggtc aatagccgcc gcgtgggcgg tattcatctg
     9421 ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa taagaggtaa
     9481 gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg caaaataccc
     9541 atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc aggcgttccc
     9601 tgagcaatca tttcaccact gcgcagggcg accagataat cacagtagcg tgccgccata
     9661 ttgatatcgt gcaacacggc aatgaccgtc aggccacgtt cctgacttaa acggtgcacc
     9721 agcgccagca catcaacctg gtgggcgata tccagcgccg aagtcggttc gtcgagcaac
     9781 agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg ttcgccgcca
     9841 gagagactat cgaccagccg atgcgccagc ggttttaagc caaccagcga gatagcttcc
     9901 tcgacctttt cgcgatctgc cgcaccaaag cgccccagcg cgccatgcca cgggtaacga
     9961 ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg ctgcggcaaa
    10021 taagccactt tgcgggcaaa cgctttgctg ctccagcttt ccagcggctg ggcatcaaga
    10081 agaatctccc cttctgacgg aggctgatga cgaccaagca ttttgagcag agtggattta
    10141 ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt taacgacagc
    10201 ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc aaaagtggta
    10261 tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaatgggca cggaaatccg
    10321 tgccccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga cgttctgcgc
    10381 cccagaagca gccataagtc tggaagcaac tggcaacata ctcacgatcg agcaaattgt
    10441 tgacgttcac agcaaggctg gatcccgcca taccaaagcg tgccagatca tattttacaa
    10501 cagcatccat tacagctgtg ctacccactt taaaagtgtt tgccggatca ccatagctgg
    10561 aaccaataaa acgaccacct gttcccaatg ttaaaccaga aagcggccct tcattgaagg
    10621 tataatcccc ccagagagat gccatatgct ccggtacttg ttctggagtt ttacctttga
    10681 gattggtgtc tttggtgtat tccgcatcgg tgtaggtata agaagccgtc aagttaatat
    10741 tggcatttaa cgccgctttt gcttcaagtt ccacaccacg agcgcgaatc tcacctgctg
    10801 ggacttgcgc taatgggttt gttgggtctg ctgtcaggtt atttgttttc gtcaattgat
    10861 agactgcgcc cgtaacaacg accggcatat cattcggaac atattttacg ccagcttcat
    10921 actgttcacc tttcgatggc ttataggaaa tgcgcgggtt gctccacaga tcgaaagcac
    10981 tcggttcaaa ggactggcta tagctaaagt aaggtgaaat accattatcg aataagtaat
    11041 ttacaccacc gcgccaggtg aactgatgat cattgcgctc aatataacct tcagtcggcg
    11101 tataagagtt ttcacgaaca gtggtcgctt gcttagacca atcgtagcgt ccccccagag
    11161 tgaataccca tttattccat tccgcctgat cctgaacata aatacctgtt tgtttgcttt
    11221 cattcatctg gtatggctct gcgctaccaa atgcaaagta ttcagggtga tatttgttat
    11281 aaagatcgat ggatggtgcg gatccaaagc tggcattgat atcattacgc atccgcatga
    11341 agtctacccc tgtcagcaaa gtatgctcga cgtcacctgt agcaaactta ctttcaagtt
    11401 gggtatcaac gctaaagttt tgtagacgct cattatccac cactgtcccg cggtttaggg
    11461 tatgaccatc accagcaatc cctgtgccat aaacactttt ctgtgaggtt ttcatttcac
    11521 tgaaacgcag attctgacgc acggtgaagg tgtcattaaa accatgttca aaactatatc
    11581 ccaccatttt ctggttacgg gagtatgtat tattcgatgc accttcattg aagtcagtcg
    11641 gtagacgctt accattaggc aatggctcaa cggtcccctc tttcggcaac caaccgtaat
    11701 aacccgtttc tggttcattc tggaaatagg acaggaaagt aaaattggtt ttgtcgtctg
    11761 gacgccatga gaatgatgga gcaatggtat aacgctgaga ttcagagttt ttctgctgtt
    11821 cgtttgttga gcgagcgagg cccgtcaaac ggtaagagaa ctcaccgtta tcatccagtg
    11881 catcactgaa atcgaatccg gtctgaaaca gattatccgt ccccatttta aactgaattt
    11941 ctttcagggg ttcagtggtc ggacgcttac tcaccataga aataatccca ccaggattac
    12001 tttttccata gagaacagac gtcggcccac gcatcagttc aaccctctcg agcatgtaag
    12061 gatcaatcac agcatcgtta taaaagttgc cctgaagttt tagtccatca aggtaattat
    12121 tttgattcag accaacagat gagaaaccac gaatgataac aaaatcatat gtattagaag
    12181 caccacgact gctaaccgta acgccaggtg tataacctaa tgcttctttt acagactgaa
    12241 attgacgcat ctgcatctct tcatttgtca caaccgaaat agactgcggc gttttttcaa
    12301 ttggtgtatc cgttttagtc gcagtagcag agtgcttcgc cgcaatagtt gcagcaggcc
    12361 cccatgcgct ttcttgcggc gcaggtgcag cggtaacggt gatagtgtct tctttcggtt
    12421 caaccgctgc ctgtgcataa acagacatgc cgctaaccgc tgtggctact acaactgcga
    12481 ttttacgcag tgagtgtttt ggctgagcag ttttggaacg cgccattggt atatctctga
    12541 tgtaaagtga atgataacgt aaacgagaat gattattata aagataagat aatattcgaa
    12601 acgctggcgg gttcgcaagc ggtacgcaat ctgagcgttc ctgaagggtt aataaacaac
    12661 cagatgaaaa gaaagggtta atactttagg tgggatgtta ttttaccgga tggcaattcg
    12721 ccatccggta tttcacgctt agatgttaat tactaccaaa catatccttg atccaaccgg
    12781 ctacaccgtc gctgtctttc tgctcttgct gagcaggttg ctgttgcggc tgttgctgcg
    12841 gctgagaaga ctgatcaaac ggattgcctg acggctgctg ctgcatctcg ctctgctggc
    12901 acagcgattg cggatcgctg gtccagaccg gcaagacacg catgccaccg ctgcaaacaa
    12961 agttgccgtc gtagtccacg cccatatctg caatatcttc tggcggaaca agattcagcg
    13021 gcgttggcgt ctggttagcc agataacgct gataaataga cattgccccg ctggcaccat
    13081 acagtttggt cggctggttg ttatcacggc ctacccaggt gatggtcacc gtgctgccgt
    13141 caatgcccgc aaaccaggta tctacgttat tgttggtagt ccctgttttc cctgccagat
    13201 gcaggttcgg gtatttcgcc ccaagttgac gacccgtacc acgttgtacc acctgctgca
    13261 tggtccatag tgtcagatac gccgcctgcg ccggaacagc gcgttctgcc tgcgggaagc
    13321 tctgatacag caccttgcca tcttccgcga ttaccgaacg cagtgcagaa agcggtgcac
    13381 ggttaccgcc gctggcgatg gtctggaatg cctgcgccac ttcgattggc gttaagttca
    13441 acgcccccag cagcatcgcc ggaaccggat gcagctgatc tttcggcacg cccagtttga
    13501 tccaggtctc cgtaactgca ggcagcccca gcgccatccc cagatttacc gtcggcacgt
    13561 tcatcgaacg ggtcaacgca tccaccagca tcactctgcc gctttcgcta taacgacggt
    13621 catcgttctg cggtgaccag acctggccat tcggctgacg cagcgcgatt ggcgcatcgg
    13681 caatccacgt gttcaggcga tagattttcg gctggcttaa agccgtcaga taagtcgctg
    13741 gttttgcaag ggaaccaatc gaacgacgcg cctgcatcgc acggttgtag cccgcaaact
    13801 gcggctcaga acctcccacc atcgcacgaa cttcaccact aaagcggtcg acgaccacaa
    13861 ttgcagtttc aagatcgctc aacttacgct gtttcttcag tgccggaatg ccttccacgg
    13921 cggctttttc tgccgcgtcc tgggccaccg agtcaaaggt agtgaagatc ttcacgccgg
    13981 agagatcttt taccttatcg cctaatttcg cctgcagctc ctgacgcacc agttgcataa
    14041 aggctggctg tggagagatt accccaccgc gcggctgtac gcccagagga cgcgcgctga
    14101 gcatttcata cagttcacca tcgataatct gctgttgttg tagcaaacgc agcaccagat
    14161 tacgtcgctc cagcgccagt ttcgggttac gccacgggtt gtagatggac gcccctttca
    14221 ccataccgac taacagcgcc tgctggtcga ggctgagctc ttctaccggg cgaccaaagt
    14281 aatacaagct cgcaagcggg aagccgcgga tttcgttgtc gccgctctga ccgagataca
    14341 cctcgttcat atacagctca agaatacggt ctttgctgta acgcgcgtcc atgatcagcg
    14401 ccatgtaagc ttcgttcgct ttacgccagt aagaacgctc gctggagagg aacaggtttt
    14461 tcaccagctg ttgcgtcagc gtactcgctc cctgcaccgt gcgtccggcg gtcaggtttg
    14521 ccagcaccgc acgtccgatg gagtagagac tgatcccatc atgctcgtag aaatgacggt
    14581 cttctgtcgc cagcaaagta tccaccagca agtccgggaa accactgcgc ggcacaaaca
    14641 gacgctgctc accgtttggc gaagagatca tggtgatcag gcgcggatca agacggaaga
    14701 aaccgaactg acggttgttc tccatattga cgatcgtcgc cagatgatcg ccatcaaagg
    14761 tcagacgcgc acgcacctgc ccttctttgc tgtccgggaa atcaaacgga cggcggatca
    14821 tctcaatgct gttggcctgc acggtaaatt cgccaggacg ggtcattttc gacacctgac
    14881 gatactgtgt tgcctccagc agcttcacca tttcgttctt gctgatggtc atatctggtt
    14941 caaggttgac cattcggcca taaactgccg cgggcagttg ccagaccttg ccatcaatac
    15001 ggctacgaat tttttgatcg agataaacgc cgtaaatggc gatcagcacg gcaaaaacga
    15061 tagccagttt taacagcagc catagccagc cgcgtttgcc acgaggctta cgccctttgc
    15121 ctttgccctt acctttgcgc ggcatcggtt cttcatcctc atagtcatca taatcgtcgt
    15181 aatcatcgtc atcttcgtaa cgacgacggc ttaccttttg tttgaccgga cgcgtcggtt
    15241 tccctttgcg tccaattggc tcgcggtcat tcccggccat gctttttctc cgcaatattc
    15301 aggcgcaaaa gcccgatttt ctgttcttct gttacaagac agaagaagat atctctcaaa
    15361 atttgtgctc gatcggtccc ctctcccttg agggagaggg ttagggtgag ggtgaacgtg
    15421 cgcgctgatg ccctcacccc ggccctctcc cacagggaga gggagaaaac caaccttacg
    15481 aatacttttt cgtccgtcgc gtcggtgccg tgtttgccgg gtcgtccggc cagacgtgtt
    15541 tgggataacg ccctttcatc tctttttgca cctcacggta cgctcctttc cagaaggcgc
    15601 tcaaatctcg tgtgatttgt agtggccttt gggcgggtga aagcaactcc agcaccagcg
    15661 gcacgccccc ctgggcgata gtcggattag tggcctcacc aaacatctcc tgcattctca
    15721 ccgccagtgc gggcgggtta tcttcatgat aacgaatggc gatccggctt cccgtcggca
    15781 cagtgtaatg cgcaggcaat tcactatcca gacgttgctg cattccccaa tcaagtaatc
    15841 cgcgtagtgc ctgataaatg tccagtgatt tcaggccgcg tagtgaatgt acgcctgtca
    15901 tatgtggcag cagccacgtt tccagcgctg ccaataaact ttcatcatca accgctggcc
    15961 agtcatattc cggcaaccac tttgcggcgc ataacaaacg caagcgtagc tgttccgctt
    16021 ccgccgtcca gttgagcacg cttaaacctt tatcacggat gccattaagc atcgcctgat
    16081 gcaactcgtc ttctgacggt ttcgccagcg gctgcacttt caccgtcaac tgaccgattt
    16141 gtagccgacg ccaggctttc agcgtacctt gcgcgtcatc ccattccacg gtgtcagatt
    16201 gctgcaccag ctgcggacag cgttgtacta attcatcaat atcgaccggt agcgccagta
    16261 aaatccgcgc atccggcgag gcgctgccct gcaataataa cggtgcgatc aaccattcgt
    16321 ggcggcttag cgcgtcgtcg gcatcgagca tcgctcccat gccgtttgcc agttgatagc
    16381 gtccatcttg cccacggcga tgagcaatgc gatcggcaaa cgccccggca agtagcggcg
    16441 cgataagcga actgtctgcc tcaccgccgc gtacgtttaa gcgttttaac agttgctgac
    16501 tacgttgctg ccaggctggt tgattgcgcg aaaacgccac gcccaggtca ctattgccca
    16561 tcagtggcgg ctcttcgaga atggcggcaa tttttgccgc ggtagcagct tcgtcgtcgt
    16621 tcttcgcgct aaccagcatt gccgctaaac gcggatcgtt acccagcgct gccatttttt
    16681 gcccttgcgc actaagccgt tcaccgtcca gcgcccccag catttgtaac agacgtttcg
    16741 cggccattag attcactact ggcggttgat ccagccagct catctgcgcc ggatcgctgc
    16801 atccccattg cagtaattcc atcagcaaac cggaaagatc gctttgtaag atctccggtt
    16861 cactttgcgc ggcagcgcgt tctgcttgtt ctttggcgat taaatgcagg cagatacccg
    16921 gctccagacg cccggcgcgc ccggcacgct gcgtcatgga tgcctggcta acgcgttgag
    16981 taatcagtcg cgtaagcccc gtgcgcggct caaaacgcgc cacacgctcc tgggcacaat
    17041 ccaccaccag acgaatacct tcaatggtta aactggtttc agcaatattg gtcgccagca
    17101 ccactttgcg catcccttgc ggtgccggga ggatcgcttt tcgctgatcg ttcagcgaca
    17161 acgcgccata cagcgggcag agcaacacat cactgccgat gcgcgaagcc agttgttcct
    17221 gcacacgctg aatttctccg acgccaggta aaaataacag taatgatccg ctttcctgac
    17281 gcagcatttc ggcggtggct accgcaacgg cttcgtcaaa acgctgatgc gcgggcagcg
    17341 gtaaataatg gcgttcaacc ggaaacgagc gcccttctga gatgacgacg ggcgcttctg
    17401 gcagcatttg ctgcaagcgg tcgttatcca gcgtagccga cataatcagc agtttaaggt
    17461 catcacgcag accttgttgc acatcgagta acagcgccaa cgccaaatcc gcctgcaagc
    17521 tgcgctcatg aaattcatca aggatcacca atccaacacc gctcaactcc gggtcacgct
    17581 ggatcatccg cgtcagcacg ccttcggtaa ctacttccag acgggtattt ggcccgacgc
    17641 agttttgcgc acgcatccgg tagccaacgg tatcgcctgg cttttcgtta agcagctccg
    17701 ccagccgttg cgcgacgtta cgtgccgcca gacgacgcgg ctccagcagg ataattttcc
    17761 cgttaatgcc gggatgcgcc agcagttgca gcggcagcca ggttgatttc ccggccccgg
    17821 tcggcgcact taataatacc tgcggcgcac aatcgagagc ggtaagtaat tcaggtaaga
    17881 cggcagcaac gggcaacgac gacacaaaac gctccagagg gttaacattc ttcgcgccac
    17941 attgtagcat cgcggtaatt cataaccgag tgcctcacat gtctgaaccg caacgtctgt
    18001 tctttgctat cgacttacct gcagaaatcc gcgaacagat tatccactgg cgcgccacac
    18061 acttcccacc tgaggcggga cgtccggtcg ccgccgataa tttgcatctg actctggcat
    18121 ttttaggcga agtgagcgcc gagaaagaga aggcgctttc tcttttagcc ggacggattc
    18181 gtcaacctgg tttcacactc acgcttgatg acgccggaca atggctgcgt tcgcgtgtgg
    18241 tgtggttagg gatgcgtcag ccgccacgcg gcttaatcca gctggcgaat atgctccgtt
    18301 cacaggctgc ccgcagcggt tgttttcaaa gcaatcgtcc gtttcatcca catattacct
    18361 tattgcgcga cgccagcgag gcggtgacaa tcccgccgcc aggttttaac tggtcttatg
    18421 cggtgacgga gttcaccctt tacgcctcct cgtttgcccg tggacgcaca cgctacacgc
    18481 cgctaaaacg ctgggcgcta acgcaataac aaggattgtc gcaatggaat tctctccccc
    18541 tctacagcgc gcgacgctaa ttcagcgtta caaacgtttt ttagccgatg tgatcacacc
    18601 cgatggtcgc gaattaacgc tacactgccc gaatacgggt gcgatgaccg gttgtgcaac
    18661 gcgtggcgat accgtctggt attcgacttc agacaacacc aaacggaaat acccacacac
    18721 ctgggaacta actcaaagcc agagcggcgc atttatttgc gtcaatacgc tttgggctaa
    18781 caggttgacg aaagaggcta tccttaatga atcaatttca gaactgtcag gctatagctc
    18841 gctgaaaagc gaagtaaaat acggcgcaga acgcagccgt attgacttta tgttgcaggc
    18901 ggattcgcgt ccagactgct atattgaagt gaaatcggtt acgttagcgg agaacgaaca
    18961 gggatatttt cccgatgcgg tcactgaacg aggtcagaaa caccttcggg agttgatgaa
    19021 cgtagcggct gaaggccagc gtgcggttat ctttttcgcc gtgctgcatt cagccattac
    19081 acggttttca cccgcgcgcc acatcgatga gaaatacgcg caactattgt cagaagctca
    19141 acagaggggg gtagaaattc tggcttacaa agcggaaatt tctgctgaag gcatggctct
    19201 taaaaaatca ctgtcggtta cattgtagta aagtaagtaa ctggttaatt tacattctgg
    19261 tcgcgtgcgc aaatacgctt ttcctcacac agttgtcaag tgttacgttt agataattgc
    19321 tatccggaaa agcatctgct atttatagcg gcctcatttt tcccccgaac atggggatcg
    19381 atagtgcgtg ttaaggagaa gcaacatgca agaagggcaa aaccgtaaaa catcgtccct
    19441 gagtattctc gccatcgctg gggtggaacc atatcaggag aagccgggcg aagagtatat
    19501 gaatgaagcc cagctggcgc acttccgtcg tattctggaa gcatggcgta atcaactcag
    19561 ggatgaagtc gatcgcaccg ttacacatat gcaggatgaa gcagccaact tcccggaccc
    19621 ggtagaccgt gcagcccagg aagaagagtt cagcctcgaa ctgcgtaacc gcgatcgcga
    19681 gcgtaagctg atcaaaaaga tcgagaagac gctgaaaaaa gtggaagacg aagatttcgg
    19741 ctactgcgaa tcctgcggtg ttgaaattgg tattcgccgt ctggaagcgc gcccgacagc
    19801 cgatctgtgc atcgactgca aaacgctggc tgaaattcgc gaaaaacaga tggctggcta
    19861 attacagccg ttccatcacg tttaccacag gcgggaaatg ctcccgcctt attttttgtt
    19921 caaagagatg acagacacac agtatattgg ccgcttcgcc ccctctcctt ccggcgagct
    19981 tcattttggc tctctgatcg ccgcgctcgg cagctatttg caggctcgcg cccggcaagg
    20041 tcgctggctg gtacgcatag aagatatcga cccgcctcgt gaagttcccg gtgccgcaga
    20101 aactatcctg cgccagctgg aacattacgg tctgcactgg gacggcgatg ttctctggca
    20161 atcgcaacgt cacgacgcct atcgtgaagc actcgcctgg ttacatgaac aaggactaag
    20221 ttattactgc acctgtacgc gtgcgcgtat tcaaagcatt ggcggtattt acgacggtca
    20281 ttgccgggtg ttgcatcatg gaccagacaa tgccgcagtg cgtatccgcc agcagcatcc
    20341 ggtcacgcaa tttactgacc agctgcgcgg cattattcac gccgacgaaa aactggcacg
    20401 ggaagatttt atcattcatc gccgtgatgg gttgttcgcc tacaacctgg ctgttgtggt
    20461 tgatgatcat ttccagggcg ttacagaaat agtgcgtggg gctgatctga ttgaaccaac
    20521 agtaaggcaa atctcgctgt accagctttt tggctggaaa gtgccagatt acattcatct
    20581 gccgctggcg cttaatccac aaggcgctaa actttccaag cagaatcatg cgcctgcgtt
    20641 gccgaaaggc gatccacgtc cggtactaat cgcggcactt caatttctgg gtcagcaggc
    20701 agaagcacac tggcaggatt tcagcgtcga acaaatcctt cagtcagcag tcaaaaactg
    20761 gacgctgacc gccgtgcccg agtcggcaat tgtaaattca acattctcaa atgcgtcatg
    20821 ctgagctatg attagccgct atttttttgt cctgaatgat gtttgacact accgaggtgt
    20881 actattttta cccgagtcgc taatttttgc cgcaaggtgc taagccgcga ggaaagcgag
    20941 gctgaacagg cagtcgcccg tccacaggtg acggtgatcc cgcgtgagca gcatgctatt
    21001 tcccgcaaag atatcagtga aaatgccctg aaggtaatgt acaggctcaa caaagcggga
    21061 tacgaagcct ggctggttgg cggcggcgtg cgcgacctgt tacttggcaa aaagccgaaa
    21121 gattttgacg taaccactaa cgccacgcct gagcaggtgc gcaaactgtt ccgtaactgc
    21181 cgcctggtgg gtcgccgttt ccgtctggct catgtaatgt ttggcccgga gattatcgaa
    21241 gttgcgacct tccgtggaca ccacgaaggt aacgtcagcg accgcacgac ctcccaacgc
    21301 gggcaaaacg gcatgttgct gcgcgacaac attttcggct ccatcgaaga agacgcccag
    21361 cgccgcgatt tcactatcaa cagcctctat tacagcgtag cggattttac cgtccgtgat
    21421 tacgttggcg gtatgaagga tctgaaggac ggcgttatcc gtctgattgg taacccggaa
    21481 acgcgctacc gtgaagatcc ggtacggatg ctgcgcgcgg tacgttttgc cgccaaattg
    21541 ggtatgcgca tcagcccgga aaccgcagaa ccgatccctc gcctcgctac cctgctgaac
    21601 gatatcccac cggcacgcct gtttgaagaa tcgcttaaac tgctacaagc gggctacggt
    21661 tacgaaacct ataagctgtt gtgtgaatat catctgttcc agccgctgtt cccgaccatt
    21721 acccgctact tcacggaaaa tggcgacagc ccgatggagc ggatcattga acaggtgctg
    21781 aagaataccg atacgcgtat ccataacgat atgcgcgtga acccggcgtt cttgtttgcc
    21841 gccatgttct ggtacccact gctggagacg gcacagaaga tcgcccagga aagcggcctg
    21901 acctatcacg acgctttcgc gctggcgatg aacgacgtgc tggacgaagc ctgccgttca
    21961 ctggcaatcc cgaaacgcct gacgacgtta acccgcgata tctggcagtt gcagttgcgt
    22021 atgtcccgac gtcagggtaa acgcgcatgg aaactgctgg agcatcctaa gttccgtgcg
    22081 gcttatgacc tgttggcctt gcgagctgaa gttgaacgta acgctgaact gcagcgtctg
    22141 gtgaaatggt ggggtgagtt ccaggtttcc gcgccaccag atcaaaaagg gatgcttaac
    22201 gagttggatg aagagccgtc accgcgtcgc cgtactcgtc gtccacgcaa acgcgcaccg
    22261 cgtcgtgagg gtaccgcatg acagtggcgt atattgccat aggcagcaat ctggcctctc
    22321 cgctggagca ggtcaatgct gccctgaaag cattaggcga tatccctgaa agccacattc
    22381 ttgccgtttc ttcgttttac cgcactccac cgctggggcc gcaagatcaa cccgattacc
    22441 tcaacgccgc cgtggcgctt gaaacttcgc tcgccccgga agagttactc aaccatacac
    22501 agcgcataga actgcaacag ggtcgtgttc gtaaagctga acgctgggga ccacgcacgc
    22561 tggatctcga tatcatgctg tttggtaatg aagtgataaa taccgaacgc ctgaccgttc
    22621 cgcactacga tatgaagaat cgtggattta tgctgtggcc gctgtttgag attgcaccgg
    22681 agctggcgtt tcctgatggg gagacgttgc gtgaagtctt acatacaaga gcatttgacg
    22741 aattaagcaa atggtaattt atttatatta cgaatataaa tattttctga aaatcataca
    22801 tcctctcttt ccttaataaa aattttagtt taaacatttt tgcagaaaca taatataaat
    22861 tcccgcagat aaatatcaat cccatcgcca taccctaacc aattgcactt gtgaatttat
    22921 ttttgcttaa ttttgaatta agaaaattta atttgttcaa aagcccttaa agtgcacaat
    22981 aggcgtccta atgaacaatg gttgtaaatt ttggtaacaa atatcattgc tcattaatat
    23041 ctagcatctg cttataacat ttgtgtcatg aatagtcatt cttcaaacca gtagtctgac
    23101 taatggaatt ttattcaagg atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc
    23161 ttatgctggc aatggttgca ggtactgctt ctgctgatat ggatggtggt cagttaaata
    23221 tcagtggtct ggttgttgat aacacctgtg aaacccgcgt tgacggcggt aataaagatg
    23281 gtctgatcct gctgcaaact gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca
    23341 ccgttggcgc taaagccaaa cctttcagca tcaccgttga ctgcagcaaa gcaaatccga
    23401 atccaggtag tacagctaaa atgaccttcg gttctgtttt ctttggtaat agcaaaggaa
    23461 cgctgaacaa cgacatgtct attaacaacc cgtctgacgg cgtgaatatc gctctgcaca
    23521 atattgatgg ttccactatc aaacaggttc aaattaacaa ccctggcgat gtgtatacta
    23581 aagccctgga tgcaacgaca aaatctgctg tttatgattt taaagcgtct tacgttcgtg
    23641 ccgtcgcaga ccaaacagca actgctggtt atgtaaaaac taacactgca tacaccatta
    23701 cttatcagta agtattcacc aattaaattg attgaatgaa tatacaggga atagtgttaa
    23761 taatttctat ttttatatta ttccctgttt taattaactc tgtcagggat ggtttatgtt
    23821 ttttaacacc aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc
    23881 gtctattgcg gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag
    23941 tgtcaacgta cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga
    24001 tactggcgat aacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc
    24061 agtatcgcgt attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac
    24121 ctcactgcct aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa
    24181 accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat
    24241 aaaacttttc tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct
    24301 gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc
    24361 ttactacgtc tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga
    24421 tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa
    24481 agcaagttct gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg
    24541 taatgccagt ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa
    24601 aaccaactaa aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt
    24661 attgcaatag tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc
    24721 agaatgcctc aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta
    24781 tgtacgacgt cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta
    24841 ttgaaattga aggaaaaaaa acgcacaagc ctgtatcaca cttaagaatt tattgcagtt
    24901 tcatataaat tctcccgata taaataacga aaaagccgtt ctgcttgcca gggatgaaac
    24961 actcggcaat tgccttaatt tgacggaaat tatccctcag gcttctgttc gttatgacgt
    25021 taacgagcaa cgtctggata tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa
    25081 ctatgttgat ccatcgttat gggaaaatgg cattaatgcg gccatgttgt catacaacct
    25141 caacggatat catagtgaaa ctcctggtcg aagaaatgac agcatttatg ctgcttttaa
    25201 cggtgggatg aatttaggtg catggcgact gcgtgcctca ggcaactaca actggatgac
    25261 cgattctggc agtaattatg attttaagaa tcggtatata cagcgtgata tcgcctcgct
    25321 gcgttctcaa ctcattcttg gtgagtctta tacaacgggc gaaacctttg attctgtcag
    25381 tatccgcggc attcgtttat acagcgacag ccgcatgttg cctccgacat tagcgagttt
    25441 tgcgcctatc attcatggcg ttgctaatac caacgccaaa gtcactatta cgcaaggtgg
    25501 ctataagatt tatgaaacga ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc
    25561 gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa tccgatggct caaagcggac
    25621 attctcgcaa cctttttcat cggtcgttca aatgttacgc cctggcgttg gacgttggga
    25681 tattagcggc ggccaggtct taaaagatga tattcaggat gagcctaatt tatttcaggc
    25741 cagctactac tacggtctga ataactatct aac
//
LOCUS       DAJTYZ010000051        25494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000051 DAJTYZ010000000
VERSION     DAJTYZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.051"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            15..485
                     /locus_tag="OO596_002007"
     CDS             15..485
                     /locus_tag="OO596_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR6717869.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            487..906
                     /gene="gspB"
                     /locus_tag="OO596_002008"
     CDS             487..906
                     /gene="gspB"
                     /locus_tag="OO596_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCR6717870.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1144..1455
                     /gene="rpsJ"
                     /locus_tag="OO596_002009"
     CDS             1144..1455
                     /gene="rpsJ"
                     /locus_tag="OO596_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6717871.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1488..2117
                     /gene="rplC"
                     /locus_tag="OO596_002010"
     CDS             1488..2117
                     /gene="rplC"
                     /locus_tag="OO596_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6717872.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2128..2733
                     /gene="rplD"
                     /locus_tag="OO596_002011"
     CDS             2128..2733
                     /gene="rplD"
                     /locus_tag="OO596_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6717873.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2730..3032
                     /gene="rplW"
                     /locus_tag="OO596_002012"
     CDS             2730..3032
                     /gene="rplW"
                     /locus_tag="OO596_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6717874.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3050..3871
                     /gene="rplB"
                     /locus_tag="OO596_002013"
     CDS             3050..3871
                     /gene="rplB"
                     /locus_tag="OO596_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6717875.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3888..4166
                     /gene="rpsS"
                     /locus_tag="OO596_002014"
     CDS             3888..4166
                     /gene="rpsS"
                     /locus_tag="OO596_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6717876.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4181..4513
                     /gene="rplV"
                     /locus_tag="OO596_002015"
     CDS             4181..4513
                     /gene="rplV"
                     /locus_tag="OO596_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6717877.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4531..5232
                     /gene="rpsC"
                     /locus_tag="OO596_002016"
     CDS             4531..5232
                     /gene="rpsC"
                     /locus_tag="OO596_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6717878.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5245..5655
                     /gene="rplP"
                     /locus_tag="OO596_002017"
     CDS             5245..5655
                     /gene="rplP"
                     /locus_tag="OO596_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6717879.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5655..5846
                     /gene="rpmC"
                     /locus_tag="OO596_002018"
     CDS             5655..5846
                     /gene="rpmC"
                     /locus_tag="OO596_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6717880.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5846..6100
                     /gene="rpsQ"
                     /locus_tag="OO596_002019"
     CDS             5846..6100
                     /gene="rpsQ"
                     /locus_tag="OO596_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6717881.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6265..6636
                     /gene="rplN"
                     /locus_tag="OO596_002020"
     CDS             6265..6636
                     /gene="rplN"
                     /locus_tag="OO596_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6717882.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6647..6961
                     /gene="rplX"
                     /locus_tag="OO596_002021"
     CDS             6647..6961
                     /gene="rplX"
                     /locus_tag="OO596_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6717883.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6976..7515
                     /gene="rplE"
                     /locus_tag="OO596_002022"
     CDS             6976..7515
                     /gene="rplE"
                     /locus_tag="OO596_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6717884.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7530..7835
                     /gene="rpsN"
                     /locus_tag="OO596_002023"
     CDS             7530..7835
                     /gene="rpsN"
                     /locus_tag="OO596_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6717885.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7869..8261
                     /gene="rpsH"
                     /locus_tag="OO596_002024"
     CDS             7869..8261
                     /gene="rpsH"
                     /locus_tag="OO596_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6717886.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8274..8807
                     /gene="rplF"
                     /locus_tag="OO596_002025"
     CDS             8274..8807
                     /gene="rplF"
                     /locus_tag="OO596_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6717887.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8817..9170
                     /gene="rplR"
                     /locus_tag="OO596_002026"
     CDS             8817..9170
                     /gene="rplR"
                     /locus_tag="OO596_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6717888.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9185..9688
                     /gene="rpsE"
                     /locus_tag="OO596_002027"
     CDS             9185..9688
                     /gene="rpsE"
                     /locus_tag="OO596_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6717889.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9692..9871
                     /gene="rpmD"
                     /locus_tag="OO596_002028"
     CDS             9692..9871
                     /gene="rpmD"
                     /locus_tag="OO596_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6717890.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9875..10309
                     /gene="rplO"
                     /locus_tag="OO596_002029"
     CDS             9875..10309
                     /gene="rplO"
                     /locus_tag="OO596_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6717891.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10317..11648
                     /gene="secY"
                     /locus_tag="OO596_002030"
     CDS             10317..11648
                     /gene="secY"
                     /locus_tag="OO596_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6717892.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11680..11796
                     /gene="rpmJ"
                     /locus_tag="OO596_002031"
     CDS             11680..11796
                     /gene="rpmJ"
                     /locus_tag="OO596_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6717893.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11943..12299
                     /gene="rpsM"
                     /locus_tag="OO596_002032"
     CDS             11943..12299
                     /gene="rpsM"
                     /locus_tag="OO596_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6717894.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12316..12705
                     /gene="rpsK"
                     /locus_tag="OO596_002033"
     CDS             12316..12705
                     /gene="rpsK"
                     /locus_tag="OO596_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6717895.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12739..13359
                     /gene="rpsD"
                     /locus_tag="OO596_002034"
     CDS             12739..13359
                     /gene="rpsD"
                     /locus_tag="OO596_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6717896.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13385..14374
                     /gene="rpoA"
                     /locus_tag="OO596_002035"
     CDS             13385..14374
                     /gene="rpoA"
                     /locus_tag="OO596_002035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6717897.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14415..14798
                     /gene="rplQ"
                     /locus_tag="OO596_002036"
     CDS             14415..14798
                     /gene="rplQ"
                     /locus_tag="OO596_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6717898.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14905..15273
                     /locus_tag="OO596_002037"
     CDS             14905..15273
                     /locus_tag="OO596_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR6717899.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15284..15709
                     /gene="zntR"
                     /locus_tag="OO596_002038"
     CDS             15284..15709
                     /gene="zntR"
                     /locus_tag="OO596_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR6717900.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15765..15983
                     /gene="arfA"
                     /locus_tag="OO596_002039"
     CDS             15765..15983
                     /gene="arfA"
                     /locus_tag="OO596_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR6717901.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="OO596_002040"
     CDS             complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="OO596_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR6717902.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="OO596_002041"
     CDS             complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="OO596_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6717903.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="OO596_002042"
     CDS             complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="OO596_002042"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6717904.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="OO596_002043"
     CDS             complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="OO596_002043"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6717905.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20215..20724)
                     /gene="def"
                     /locus_tag="OO596_002044"
     CDS             complement(20215..20724)
                     /gene="def"
                     /locus_tag="OO596_002044"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6717906.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20854..21978
                     /gene="dprA"
                     /locus_tag="OO596_002045"
     CDS             20854..21978
                     /gene="dprA"
                     /locus_tag="OO596_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6717907.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21950..22423
                     /locus_tag="OO596_002046"
     CDS             21950..22423
                     /locus_tag="OO596_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6717908.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22452..22994
                     /locus_tag="OO596_002047"
     CDS             22452..22994
                     /locus_tag="OO596_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR6717909.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22999..23571
                     /gene="tsaC"
                     /locus_tag="OO596_002048"
     CDS             22999..23571
                     /gene="tsaC"
                     /locus_tag="OO596_002048"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6717910.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23576..24394
                     /gene="aroE"
                     /locus_tag="OO596_002049"
     CDS             23576..24394
                     /gene="aroE"
                     /locus_tag="OO596_002049"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6717911.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24391..24648
                     /locus_tag="OO596_002050"
     CDS             24391..24648
                     /locus_tag="OO596_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6717912.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24624..25178)
                     /locus_tag="OO596_002051"
     CDS             complement(24624..25178)
                     /locus_tag="OO596_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6717913.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6492 a         6022 c         6679 g         6301 t
ORIGIN      
        1 cttctttaca aacattggta gatcagaatt tgccctggag cgcatcgctt aaagtcgggg
       61 acaaaaaatt gcctgttgtg gtcgttcgcg tcggtgaggc tagcgttgat gtcctcgtag
      121 gtcagcaaac gtggacgctg acacataaat ggtttgagtc ggtatggacg ggtgattatc
      181 ttctgttgtg gaaaatgtcg ccggaagggg agagtaccat tacgcgtgat agcagcgagg
      241 aagagattct ctggctggaa acgatgttaa accgtgcatt gcatatttcg acagaacctt
      301 cggcggagtg gcgtcccttg ttggtagaaa aaataaagca atttcaaaaa agccaccact
      361 tgaaaactga cggtgttgtt ggcttttcaa cgttagtaca tctctggcag gtggcagggg
      421 agagtgccta cttatatcgg gatgaagcaa atatttcccc ggaaacaaca gtgaagggga
      481 aatgacatgt ttgaattcta tatcgccgcg cgtgagcaga aagaaacagg tcaccctggt
      541 atcttctccc gacaaaaaca cagcaccata atatatgtca tttgtttatt gctgatttgc
      601 ctgtggtttg caggaatggt gttggttggt gggtatgcca ggcagctatg ggcgctttgg
      661 atagtaaaag cagaagtcac tgtagaggct gaaacgcccg cttttaaaca atcaacacaa
      721 cactatttct tcaaaaaaca gccactcccc gtcgtcgaat ccgtcgagga ggaagatgac
      781 ccagacgtcg cggttgagaa tgctccctct tcttctgaag acgaagaaaa tactgtaaaa
      841 gaaagcgacg agaaagccgg tttaagagag cgtgtcaaaa atgcgctgaa cgaacttgag
      901 agataacccg aaggctgttt actttactag caatacgctt gcgttcggtg gttaagtatg
      961 tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa
     1021 tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca
     1081 tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt
     1141 ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat
     1201 caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg
     1261 atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa
     1321 gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca
     1381 accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag
     1441 atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag
     1501 tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa
     1561 ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct
     1621 accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag
     1681 ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg
     1741 ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgaggtta
     1801 aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct
     1861 ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt
     1921 ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga
     1981 tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca
     2041 acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac
     2101 cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg
     2161 ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt
     2221 gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa
     2281 gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt
     2341 tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac
     2401 cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa
     2461 ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact
     2521 aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt
     2581 gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat
     2641 gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat
     2701 gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc
     2761 tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg
     2821 tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt
     2881 ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg
     2941 gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc
     3001 agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt
     3061 taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct
     3121 gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa
     3181 caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat
     3241 tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga
     3301 tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat
     3361 cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat
     3421 caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt
     3481 agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat
     3541 cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt
     3601 agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt
     3661 tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc
     3721 gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc
     3781 ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga
     3841 taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc
     3901 tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa
     3961 gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga
     4021 tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt gtgaccgacg
     4081 aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg
     4141 ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta tcgctaaaca
     4201 tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa
     4261 gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtactggt
     4321 caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga
     4381 cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc
     4441 gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt
     4501 gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt
     4561 cgcttgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct
     4621 gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg
     4681 tctgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact
     4741 gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc
     4801 gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa
     4861 ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc
     4921 cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa
     4981 gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa
     5041 ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac
     5101 accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt
     5161 atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa
     5221 ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg
     5281 cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt
     5341 ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct
     5401 atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg
     5461 atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg
     5521 gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg
     5581 gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact
     5641 aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca
     5701 ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc
     5761 agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt
     5821 tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt
     5881 tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc
     5941 gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga
     6001 atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc
     6061 ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata
     6121 cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt
     6181 tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt
     6241 agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac
     6301 aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac
     6361 gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc
     6421 aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg
     6481 gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag
     6541 cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc
     6601 atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat
     6661 ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt
     6721 taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca
     6781 tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat
     6841 tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt
     6901 tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta
     6961 atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa
     7021 ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc
     7081 ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca
     7141 gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca
     7201 ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc
     7261 atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtattcg tgacttccgt
     7321 ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag
     7381 atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc
     7441 attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc
     7501 ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag
     7561 taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca
     7621 tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc
     7681 tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgcccgc
     7741 atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg
     7801 gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt
     7861 aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc
     7921 aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca
     7981 acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg
     8041 aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg
     8101 tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg
     8161 gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc
     8221 aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc
     8281 gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg
     8341 ttattacgat caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag
     8401 ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg
     8461 cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct
     8521 tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga
     8581 ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg
     8641 ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgt tgataagcag gtgatcggcc
     8701 aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc
     8761 gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg
     8821 ataagaaatt tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg
     8881 gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac
     8941 cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac
     9001 tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg
     9061 ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc
     9121 gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa ggtagaggtg
     9181 taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa
     9241 ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc tgactgtagt
     9301 tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc
     9361 gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg
     9421 cactctgcaa caccctgtta aaggtgttca cacgggttct cgcgtattca tgcagccggc
     9481 ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg
     9541 ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc
     9601 aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca agcgtggtaa
     9661 atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa ttactcaaac
     9721 ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg
     9781 tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta tgatcaacgc
     9841 ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc tgtctccggc
     9901 cgaaggctcc aaaaaggcgg gtaaacgctt gggtcgtggt atcggttctg gcctcggtaa
     9961 aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg
    10021 tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct tcacttctcg
    10081 taaagcagcg attacagccg aagttcgtct gtctgacctg gctaaagtag aaggcggtgt
    10141 tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg agttcgcgaa
    10201 agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc gtgttactaa
    10261 aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag tagcagatgg
    10321 ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca
    10381 gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt attccgatcc
    10441 ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc accatcattg
    10501 agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt gctctgggga
    10561 tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt cacccaacgt
    10621 tggcggaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag tacacccgct
    10681 acggtactct ggtgctggca atattccagt cgatcggtat tgctactggt ctgccgaata
    10741 tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc accgctgttg
    10801 taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatt actgaacgag
    10861 gtatcggcaa cggtatttca atcattatct tcgtcggtat tgtcgcggga ctcccgccag
    10921 ccattgccca tactatcgag caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt
    10981 tggttgcagt attagtattt gcagtgacgt tctttgttgt atttgttgag cgtggtcaac
    11041 gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtttat gctgcacaga
    11101 gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca
    11161 gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact ggttggaact
    11221 ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg ttactctatg
    11281 cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa
    11341 cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa
    11401 cggcgaagta tatcgataaa gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta
    11461 cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg
    11521 gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct caagtgcaaa
    11581 ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg aaaggctacg
    11641 gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc
    11701 aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc
    11761 agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa
    11821 agttgggttg agctggctag attagccagc caatcttttg tatgtctgtg cgtttccatt
    11881 tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag taggagtgca
    11941 tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta atcgcattaa
    12001 cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca gcgggtatcg
    12061 ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg cgtgacgaag
    12121 ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc
    12181 tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc
    12241 agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc aagaaataat
    12301 cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa
    12361 gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt gactatcact
    12421 gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt ccgtggttct
    12481 cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa
    12541 gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct
    12601 actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga tgtgactccg
    12661 atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct cgttttccag
    12721 gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct gagccgtcgt
    12781 gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt
    12841 gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag
    12901 ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac
    12961 tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg
    13021 gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg tgcagaagca
    13081 cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa catcgcttct
    13141 tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc
    13201 gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacttggct ggaagttgat
    13261 gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct gtctgcggac
    13321 attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc aaagagagga
    13381 cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata tcgagcaagt
    13441 gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg gccatactct
    13501 gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt
    13561 tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg aagatatcct
    13621 ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag atgaagttat
    13681 tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca cccacgacgg
    13741 tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc
    13801 tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat
    13861 tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc
    13921 tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta ccgacctgga
    13981 caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg
    14041 tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg atgtacgtca
    14101 gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga
    14161 tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc actatatcgg
    14221 tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc
    14281 tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca tgcgcctgga
    14341 aaactggcca ccggcaagca tcgctgacga gtaaccggat cacaggttaa ggttttactg
    14401 agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca actgaaccgc aacagcagcc
    14461 atcgccaggc tatgttccgc aatatggcag gttcactggt tcgtcatgaa atcatcaaga
    14521 cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga gccgctgatt actcttgcca
    14581 agactgatag cgttgctaat cgtcgtctgg catttgcccg tactcgtgat aacgagatcg
    14641 tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc
    14701 gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg
    14761 ttgatcgttc agagaaagca gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc
    14821 cgccccggcg ggttttttta tacccgtagt atccccactt atctacaata gctgtactct
    14881 ttttgttcat cccctggagt atttatgtgg ttacttgacc agtgggcaga gcgccatata
    14941 gcagaagcgc aagcgaaagg tgagtttgat aacctggcag gtagcggcga accattgata
    15001 ctggatgatg attctcacgt gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat
    15061 gccggttgct taccgccaga acttgagcaa cggagagaag caattcagct tctggatatt
    15121 ctcaaaggta tccgtcacga tgatccgcaa tatcaagagg ttagccgtcg attgtcatta
    15181 ctggaattga agctgcgaca agctggattg agtaccgatt ttttacgcgg cgattatgct
    15241 gacaagttgt tggacaaaat caacgataac tagtggagta tgtatgtatc gcattggtga
    15301 gctggcaaaa atggcggaag taacacctga cacgattcgt tattacgaaa aacagcagat
    15361 gatggagcat gaagtgcgta ctgaaggcgg gtttcgcctg tataccgaaa gcgatctcca
    15421 gcgattgaaa tttatccgcc atgccagaca actaggtttc agtctggagt cgatccgcga
    15481 gttgctgtcg atccgcatcg atcctgaaca ccatacctgt caggagtcaa aaggcattgt
    15541 gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag ttgcagagta tgcagcgttc
    15601 cttgcaacgc cttaacgatg cctgttgtgg gaccgcccat agcagtgttt attgctcgat
    15661 tcttgaagct cttgaacaag gggcgagtgg cgttaagagt ggctgttgat tttttgcact
    15721 gacaggatta cactcgcgcc gttaaataac caactggagt ttttatgagc cgatatcagc
    15781 atactaaagg gcagataaag gataatgcga tagaagcatt actacatgat cccttattcc
    15841 gacagcgagt agagaaaaat aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg
    15901 gcaatcgggg gaactgggag gccagtggca agaaagtaaa tcactttttt accactggtc
    15961 ttctgctttc aggcgcttgt taagagcggt tattctgctc tttcagcaaa tcacgaattt
    16021 ctgccagtaa tacttcttct ttagttggtg caggtgcggc agctggttct tcttttttcc
    16081 gattcagttt gttgattagc ttaatcgcca taaagatggc aaaggccaca atcagaaaat
    16141 caaagacgtt ttgaatgaag acaccgtaat gcatcacaac agcagggata tccccctgcg
    16201 catcgcgtag cgtgacagca aactgtttaa agtcgatccc gccaattaat aagcccagag
    16261 gtggcatgat gatatcggca accagtgaag atacaatctt cccgaatgcc gcaccgataa
    16321 tgacacccac cgccaaatcc accacgttcc cgcgcatcgc aaattcgcga aattctttaa
    16381 taatgctcat gttattctcc ctatgaagcc gacaaccata agtctaacaa atgttaagcc
    16441 attttcctgc caggaatgaa tttaattaat ccttgataat caaagggaaa taataaggcg
    16501 tcattagacg ccttattaat tacaagaaga aagggcttgg ctggaagagt ctttcgacgt
    16561 cggtaataaa ctttttatct gtgaggaaca taattacgtg atcgccttgc tcaatgcgca
    16621 gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac cgctccaata atcgttcctg
    16681 gcggtagctt gatttcgtca atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt
    16741 gagcaacggc ttcaatagct tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat
    16801 ctgcttttcg cacatggcta agcaacgcag aaatagttgc ttgttgtggt gaaattgcaa
    16861 tatcgatcac gctcccctga accagatcca cataagcgcg acgctggatc aataccatca
    16921 cctttttcgc acccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt
    16981 tggtgacggc aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat
    17041 ccgacgcatc accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc
    17101 gctgctgatt acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc
    17161 ctgcaccgat attaccgcca ccaaccagca tgatccgctt atacggtttt tccagtcgct
    17221 gtaattcact catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac
    17281 cagcttcaac aatggtcgaa ccttgtggac gaatggggcg atcgtggcgg aaaattgctg
    17341 ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca
    17401 gcgggccgcc ataataggct ttaactacag cgaggctgac tttaccctca gcgaagttca
    17461 ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat attatcgata accaactgct
    17521 ctggtgcgat cagatgatca atcggtacag catctgaatg aaatagctta tcggcatcgc
    17581 gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg ggtgttgaaa agtgagtagg
    17641 ctacctggca ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg
    17701 cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgactc
    17761 gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc gttggtatcg acaacagtaa
    17821 tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca
    17881 gaatgataat tttcatcagt ctcgacccgt tatctcatca ctttttgatt agcttagcgt
    17941 aaaagaagcc gtcgccctct tcggcaccag gcagattttg tttacccggt tgctctggtg
    18001 ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa aaaagcttta atctgcaggc
    18061 tattctcttc cggtaacacc gaacaggtgg catagaccag ggttccaccg gattttaaat
    18121 gcggccaaat ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat
    18181 cgcgacgtaa ccatttaata tccggatggc gacgaatcac ccccgttgct gaacaaggcg
    18241 catctaataa aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat
    18301 caccttgttt cacggtcgcc ttcataccaa ggcgttttaa attgtcgtga acgcgagaga
    18361 ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat
    18421 gcgttgtttt accgccgggg gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg
    18481 caagccaggt catgcaacct tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa
    18541 aaccaggtag cgcatgaaca ggtgcaggtg tttccagccg tacagcatca gggtaatccg
    18601 catgcgggaa acctttcatt cctgcttcat ccaacaatgc aagccagctg tcgcgggaat
    18661 gatgcgtacg attaacgcgc agccacattg gcggacgctg gttattggct tcgacgatgg
    18721 attgccactg ctctggatac gctttttgca gacgcttcag caaccaggaa ggatgcagat
    18781 aacgtgcatc actggtatta aactcggcta ataactcttc ttgctgacgc tggaactggc
    18841 gtaatacacc gttaatcaac cctttaagtt gcggacgctt aattgcgata gcgccttcaa
    18901 ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa
    18961 ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat cggacgggcc attaacttat
    19021 taatcagcca gtctaactgc gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa
    19081 gtgctttgtc tttatcagaa actttttgct ggagcggtgg cagaatgttg cttaatgatt
    19141 gcccttgctc gacgacttgt tcaacggcct gggccgccat gctacgtaaa ttacgttgtt
    19201 ttttcataag tataaaaatg cccggtaaga ccgggcttag aagagtggac tatcaggcca
    19261 gacggttgcc cggaacaaac cattcccgac gagaattcag gaggtcttgc gcgctcatcg
    19321 ctttcttacc cgcaggttgt aacgagagca ggttcaggat gccatcacca gtcgcaacct
    19381 gaatgccttg tttgttggct tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa
    19441 tgaccgacgc tttccagact ttaaccggtt gtccttcaat ttccagccag ctcattggcc
    19501 atgggttgaa agcgcgaatg cagcgttcaa gctgcgctgc cgaaagcgac cagtcaatac
    19561 gcgcttcttc tttactcaac ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg
    19621 gtttcgccgt gccgtctgct agttgtttca acgtggtgat tagcccttgt gggccaagct
    19681 ctgccagctt gtcgtacagc gtaccactgg tatcttctgc agtaatcggg caggagagct
    19741 tatagagcat atcaccggtg tctaaaccga catccatttg cataatggtc acaccagttt
    19801 ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc accgcgccag cgtggcagca
    19861 gtgaaccatg aacgttgata cagccaagac gcggcatctc cagcactgct ttaggcagaa
    19921 ttaagccata ggcgacgacg accataacat cagcctgcag atcggcgacc agttgctggc
    19981 tttcttgtgg acgcagggaa acaggttgaa aaacgggcag acctttttcc tcagccagaa
    20041 ctttaaccgg gctgggcatc agttttttac cgcgtcctgc cggtcggtct ggctgggtga
    20101 acacgccaac gacgttatga ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt
    20161 caggtgtacc cgcaaaaata atacgtagtg attctgacac gttagttctt atccttaagc
    20221 ccgggctttc agacgatcta gtttttcaac tttctgacga atacgttgtt gtttcagcgg
    20281 tgacagataa tccataaaca gtttgccgac caggtgatcc atctcatgct gaatacagat
    20341 ggctaacagc ccctctgctt ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat
    20401 tttaactttc tctgcgcgcg gcactaaagc acgttgttca gggatcgaca ggcaaccttc
    20461 ttcaatgcct gtttcgccgc ttttttctaa aagctccgga ttgattaaca ccagccgttc
    20521 gtcacggttt tccgaaacat caataacaat gatacgttga tggatatcaa cctgggttgc
    20581 cgccaggcca ataccttctt ctgcgtacat cgtctcgaac atatcatcga cgatacgctg
    20641 tatttctgca ttcacttctt ctaccggttt agcaactttg cgaagccgct cgtccggaat
    20701 atgtaacact tgcaaaactg acataaatct ccagagatgt gttcaggggt tagaaagatt
    20761 atttcttcta ttctagacaa atccccctct gattgacagc atcactgacc aatcgcaaag
    20821 attgctaagg ctgcttatgg cagggagata aggatggtcg atacagaaat ttggctgcgt
    20881 ttaatgagta tcagcagctt gtacggcgat gatatggtcc gtatagctca ctggctggca
    20941 aaacagtcgc aaattgatgc ggttggattg cagcaaacag ggcttacatt gcggcaggca
    21001 caacgctttc tttcatttcc gcgaaagagt atcgaaagct cactttgttg gttggagcaa
    21061 cccaaccatc atttaatccc tgcggacagc gaattttatc ctcctcaact tctggtgacg
    21121 acagattacc ccggcgcact gtttgttgaa ggagaactgc acgcgctgca ttcatttcag
    21181 cttgccgtag tggggagtcg ggcgcattca tggtatggcg agcgatgggg acggttattt
    21241 tgcgaaactc tggcgacgcg tggagtgaca attacgagtg gactggcgcg tggaatcgat
    21301 ggtgtggcgc ataaagctgc cttacaggta aatggcgtca gcattgctgt attggggaat
    21361 ggacttaata ccattcatcc ccgccgccat gcccgactgg ctgccagtct gcttgaacat
    21421 ggcggagctc tcgtctcgga atttcccctc gatgttccac cccttgctta caatttccca
    21481 cgaagaaatc gcattatcag tggtctaagt aaaggtgtac tggtggtgga agcggctttg
    21541 cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc agaggcgtga agtttttgcc
    21601 ttgccaggtc caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt
    21661 gcgattcttg tgacggaacc ggaagaaatt ctggaaaact tgcaatttgg attgcactgg
    21721 ttgccagacg cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca
    21781 tttcctgagc tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa
    21841 cgtgccggcc aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga
    21901 tggatcgcag ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt
    21961 actaatgtat ttgtttgaaa cctatattca cactgaagct gagttgcgtg tggatcaaga
    22021 caaacttgaa caggatctta ccgacgcagg ttttgagcga gaagatatct acaatgccct
    22081 gctatggctg gaaaaacttg ctgattatca ggaagggttg gcagaaccga tgcaactggc
    22141 ctcggatcct ctctccatgc gtatttatac accggaagag tgtgaacgac tggatgccag
    22201 ctgccgtggg tttctgcttt tccttgagca gattcaggtg ctcaaccttg aaactcgtga
    22261 aatggtgata gagcgagtgc tcgcgctgga taacgcagag ttcgaactgg atgatctgaa
    22321 atgggtgatc ctgatggtgt tgttcaacat cccggggtgc gaaaatgcgt accagcaaat
    22381 ggaagaatta ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag
    22441 cataagttgt tatggcaaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa
    22501 agtgcggggc tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac
    22561 agtatccggc gtgtgactac gtccgtcttc tgaaatcttc agcggatgga catattgtca
    22621 aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa tctggtatta cgccagggac
    22681 gctttggtat gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg
    22741 ataaaccgga cgaaacagca attacatgcc cccaatgtcg gacgggccat ctggtccagc
    22801 gccgctcccg ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg
    22861 ccattaactt caaaccaata gctggagaat gccctgagtg tcattatccg ctactcatcg
    22921 aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg
    22981 tttcggcgga ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata
    23041 gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg
    23101 tgcgatcctg atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt
    23161 gataaggggc tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac
    23221 accatgctga ctgatgcgca gcgtgaaacc attttttccc gctggccagg tcctgtcacc
    23281 tttgtctttc ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt
    23341 gctgtacgag tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg
    23401 ctggtttcta ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt
    23461 cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat
    23521 ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga
    23581 aacctatgct gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca
    23641 atttgctcag caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga
    23701 tttcatcaac acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt
    23761 gccttttaaa gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc
    23821 tggtgcagtt aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga
    23881 tggtgtaggc ttgttaagcg atctggaacg tctgtctttt atccgccctg gtttacgtat
    23941 tctgcttatc ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctaga
    24001 ctgtgcggtg acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt
    24061 tgcgcacact ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga
    24121 tctcattatt aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc
    24181 gctcattcat ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt
    24241 tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt
    24301 ggcacaggcg gctcatgcct tccttctttg gcatggcgtt ctgcctgatg tagaaccggt
    24361 catcaaacta ttgcaacagg aattgtcagc gtgaatcagg ccatccagtt tccggatagg
    24421 gaagagtggg acgagaataa aagatgtgta tgttttcctg ctctcgtgaa tggtatgcaa
    24481 ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct ttactggagg tacgccagaa
    24541 cagtggttag cgagttttcg tcagcatcgc tgggacctgg aagaagaagc ggaaaactta
    24601 attcaggaac aaagtgaaga tgatcaaggt tgggtctggt taccttgatc cagatattcg
    24661 tccttccatt tcacgtagtt atttgcggaa tagcgtaacc cagccttctc ttcatcactt
    24721 aacgggcgga tctgtttgac gggactaccg agatacagat atccgctctc cagccgttta
    24781 ttttgtggga ccaggctacc cgcaccaatc atcacatcat cttctactat tgcgccatca
    24841 agtaaaattg agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc
    24901 accttgtgac caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac
    24961 gaggatttat gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc
    25021 tgtacataat gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc
    25081 agacgaacgt caccaatcac gacgctgcta tcgtcgatca ttacgcgctg gccgatttgt
    25141 ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac tcacctcaac aataaatgat
    25201 ttcctaatga ctttgggtgc attattagcc ttgtgcaagt cttttagtat gcaaaaaagc
    25261 accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc
    25321 aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc gcctccgttg agacgacaac
    25381 gtgaaacact tcacaggatg gtcggaacaa cgaagagaaa aaattctgaa attcagggtt
    25441 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaag
//
LOCUS       DAJTYZ010000052        25382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000052 DAJTYZ010000000
VERSION     DAJTYZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.052"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..663)
                     /gene="rlmB"
                     /locus_tag="OO596_002052"
     CDS             complement(<1..663)
                     /gene="rlmB"
                     /locus_tag="OO596_002052"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6717914.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGS"
     gene            complement(843..3284)
                     /gene="rnr"
                     /locus_tag="OO596_002053"
     CDS             complement(843..3284)
                     /gene="rnr"
                     /locus_tag="OO596_002053"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6717915.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3323..3748)
                     /gene="nsrR"
                     /locus_tag="OO596_002054"
     CDS             complement(3323..3748)
                     /gene="nsrR"
                     /locus_tag="OO596_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6717916.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3953..5251)
                     /gene="purA"
                     /locus_tag="OO596_002055"
     CDS             complement(3953..5251)
                     /gene="purA"
                     /locus_tag="OO596_002055"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6717917.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5355..5552)
                     /locus_tag="OO596_002056"
     CDS             complement(5355..5552)
                     /locus_tag="OO596_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6717918.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5634..6638)
                     /gene="hflC"
                     /locus_tag="OO596_002057"
     CDS             complement(5634..6638)
                     /gene="hflC"
                     /locus_tag="OO596_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6717919.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6641..7900)
                     /gene="hflK"
                     /locus_tag="OO596_002058"
     CDS             complement(6641..7900)
                     /gene="hflK"
                     /locus_tag="OO596_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6717920.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7986..9266)
                     /gene="hflX"
                     /locus_tag="OO596_002059"
     CDS             complement(7986..9266)
                     /gene="hflX"
                     /locus_tag="OO596_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6717921.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9343..9651)
                     /gene="hfq"
                     /locus_tag="OO596_002060"
     CDS             complement(9343..9651)
                     /gene="hfq"
                     /locus_tag="OO596_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6717922.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9737..10687)
                     /gene="miaA"
                     /locus_tag="OO596_002061"
     CDS             complement(9737..10687)
                     /gene="miaA"
                     /locus_tag="OO596_002061"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6717923.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10680..12527)
                     /gene="mutL"
                     /locus_tag="OO596_002062"
     CDS             complement(10680..12527)
                     /gene="mutL"
                     /locus_tag="OO596_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6717924.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12537..13874)
                     /gene="amiB"
                     /locus_tag="OO596_002063"
     CDS             complement(12537..13874)
                     /gene="amiB"
                     /locus_tag="OO596_002063"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6717925.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13893..14354)
                     /gene="tsaE"
                     /locus_tag="OO596_002064"
     CDS             complement(13893..14354)
                     /gene="tsaE"
                     /locus_tag="OO596_002064"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6717926.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14326..15873)
                     /gene="nnr"
                     /locus_tag="OO596_002065"
     CDS             complement(14326..15873)
                     /gene="nnr"
                     /locus_tag="OO596_002065"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6717927.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15872..17011
                     /gene="queG"
                     /locus_tag="OO596_002066"
     CDS             15872..17011
                     /gene="queG"
                     /locus_tag="OO596_002066"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6717928.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16994..17047)
                     /locus_tag="OO596_002067"
     CDS             complement(16994..17047)
                     /locus_tag="OO596_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717929.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17281..17356)
                     /locus_tag="OO596_002068"
     tRNA            complement(17281..17356)
                     /locus_tag="OO596_002068"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17321..17323),aa:Gly,seq:gcc)
     gene            complement(17514..17589)
                     /locus_tag="OO596_002069"
     tRNA            complement(17514..17589)
                     /locus_tag="OO596_002069"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17554..17556),aa:Gly,seq:gcc)
     gene            complement(17800..18345)
                     /gene="orn"
                     /locus_tag="OO596_002070"
     CDS             complement(17800..18345)
                     /gene="orn"
                     /locus_tag="OO596_002070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6717930.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18440..19492
                     /gene="rsgA"
                     /locus_tag="OO596_002071"
     CDS             18440..19492
                     /gene="rsgA"
                     /locus_tag="OO596_002071"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6717931.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19589..20557
                     /gene="psd"
                     /locus_tag="OO596_002072"
     CDS             19589..20557
                     /gene="psd"
                     /locus_tag="OO596_002072"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6717932.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20579..23902
                     /gene="mscM"
                     /locus_tag="OO596_002073"
     CDS             20579..23902
                     /gene="mscM"
                     /locus_tag="OO596_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6717933.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24053..>25382)
                     /gene="yjeM"
                     /locus_tag="OO596_002074"
     CDS             complement(24053..>25382)
                     /gene="yjeM"
                     /locus_tag="OO596_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR6717934.1"
                     /translation="EMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5856 a         7124 c         6444 g         5958 t
ORIGIN      
        1 acttcctgcc attgggatgc tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat
       61 accttcacct tccgcgccca tcaccagcgc caagcgtccg gtcattttgc tctgatagag
      121 tgtatgatcc gcctcgcctg ccgtaccgac gatccagata ttctcttcct gcaacatacg
      181 catggtgcgc gcaaggttag tcacccgaat cagtggaacg ctttctgccg cgccgcaggc
      241 tacttttttc gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcgtg
      301 aacgccagca gcgtccgcgc tacgcaggca cgcgccgagg ttgtgcggat cggttacacc
      361 gtcgaggatc agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc
      421 ctgatactga cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct
      481 tttctcgtcg agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc
      541 gtgaatcagc ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa
      601 acgttcaggg gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct
      661 cattaatgta ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gcctacatga
      721 tctctgcaat atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat
      781 ccggcatgaa caaagcgcat tttgtcagca atctaaccct cttcttttaa agagggtatt
      841 gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc ggctattttc
      901 tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc
      961 gtttttttct caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga
     1021 cgacgcttgc cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta
     1081 ccgacgttgc gcggtgcgcg ttcactggag atcagactaa agtcgatttt gcgctcgtcc
     1141 atattaaccg cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg
     1201 gattccccca tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa
     1261 gagacatgga ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca
     1321 gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac
     1381 ttcagccagt cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag
     1441 tgctgaccca gttgcaacat ctcttccatc gaataatggt agccgccggt ttcagtggtg
     1501 ttgccctgat gcccctgctc tttcgccagc agatacttaa tggcgcggtg cagcgtcagg
     1561 tcaggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc caggccaaag
     1621 tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg agcgcagcag catggtttgc
     1681 agcatttctg catcaggacg gtcggcaacc gactccagca gttccgcgta gtcacgcggt
     1741 tccggcttgt taccacccgg cagctccagc cccagctccg ccagcactga acggaaagag
     1801 gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc
     1861 tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat taatttgtgc
     1921 gcgtcgttac gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac
     1981 ttcgcttctt cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact
     2041 ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg ggacgtactg ctcgcgcaga
     2101 tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc
     2161 atcaccgctt cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg
     2221 cacaccatac acaggcggtc tacctgcggg ttgagcgaac acaggccgtt agagagcact
     2281 tccggcagca tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct
     2341 tctctgtcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat
     2401 aaacgccagc cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg
     2461 tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct
     2521 tcttccggca cttcttcttt cagccccgca acctgttgct caacagcctg cggccagata
     2581 tacggaattt catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg
     2641 cccagcactt cgacgatttt acccaccgct ttggtgcggc gagtcggacg ctgagtcagt
     2701 tcgactacga ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata
     2761 tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag
     2821 cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga
     2881 cctttacggt cagcacccag cggctgcgcc agcacctgat cgccatgaat acaggttttc
     2941 atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag
     3001 ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag
     3061 cactgacgac gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc
     3121 aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg
     3181 gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc
     3241 gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg
     3301 tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct
     3361 cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct
     3421 tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat
     3481 tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg
     3541 gtttacccag gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac
     3601 taagttgatt gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt
     3661 cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc
     3721 cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata
     3781 catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga
     3841 aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca
     3901 atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt
     3961 cgaacggatc gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga
     4021 tcggcacacc ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcaggc
     4081 cgctacgatc tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg
     4141 gctctacacc tttccagtcg tcagctgcca gcggagtggt agccacttcg cgaccatccg
     4201 gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg tccagcttgg
     4261 tcaggcaaaa gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca
     4321 gccagccggt acgacgacga cgacccgtag ttgcgccgaa ttcgttaccc tgcttgcaga
     4381 ggaactcgcc agtttcatca aacagttcgg tcgggaacgg acctgcacct acacgagttg
     4441 agtaagcttt gaggataccc agaacataat caacataacg cgggcccagg ccggaaccgg
     4501 tcgccacgcc accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga
     4561 tatccagcag cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc tgacgcgcct
     4621 ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg
     4681 tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga
     4741 agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt
     4801 cgccaacacg cagaccgcga cgtgctactt tatcttcata agcaggcccg ataccacgac
     4861 cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agcgcaacgt
     4921 gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc
     4981 cacggtcttc cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt
     5041 taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg
     5101 ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa
     5161 catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt
     5221 gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg
     5281 gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc
     5341 agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca
     5401 accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag
     5461 atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc
     5521 agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca
     5581 ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc
     5641 gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc
     5701 acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag
     5761 aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca
     5821 ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg
     5881 gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc
     5941 gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg
     6001 atctgcttga tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg
     6061 atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg
     6121 ttatctgccg ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg
     6181 tcacgtactt cgagggtcag acgaccacgg gaatcggtga cgatgtcttt cacgtccagg
     6241 cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc
     6301 gaaatgtcgc caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg
     6361 atgtaagaat cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg
     6421 tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa
     6481 tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa
     6541 cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc
     6601 agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt
     6661 agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg
     6721 acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc
     6781 tggcaccgtt atcgctcttc gccgcagggg cgttaccgcc tttcagcatc tggtctaacg
     6841 gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca
     6901 ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt
     6961 ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct
     7021 tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt
     7081 cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg
     7141 catcgtcaaa cgccgctttt acttcttccg gcggacgagc tgcctggaag ttgacgtcca
     7201 gcagagtgat acccatgtca tacggacgga tagtctcttc cagttcgcgc tgagtatcgc
     7261 tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc
     7321 cacgcagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca
     7381 gatatttttc cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct
     7441 cgtccgacgt cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg
     7501 gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga
     7561 atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg
     7621 ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg
     7681 ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac
     7741 caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc
     7801 gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt
     7861 cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt
     7921 atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta
     7981 cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca
     8041 acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct
     8101 attgcctgaa gctgataaaa acgacttctc agacgtcctt cctgcggcgg caaacgcaat
     8161 gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc
     8221 cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca
     8281 atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc
     8341 tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgt
     8401 acatccgccg catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg
     8461 gctttaaatg ctgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca
     8521 agtacggttt caccgacatc cgcgacgtca atacgccgca acgtcgggtc gagggtggca
     8581 aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta
     8641 ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc
     8701 cgcccctgct cacgctgctt ttcaactctt tctaggcgcg actgtatctg cacgatgcga
     8761 ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc
     8821 ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc
     8881 agctgcgcta gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata
     8941 tctaaaataa ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc
     9001 tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt cgctttgaca
     9061 gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta
     9121 cggctaccgg taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac
     9181 tcctggaggt cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc
     9241 tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg
     9301 caggatcgct ggctccccgt gtaaaaaaaa cagcccgaaa ccttattcgg tttcttcgct
     9361 gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc
     9421 gccaccggcg ttgttactgt gatgagaaac cgggcgagac gggacaacag tagaaatcgc
     9481 gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga
     9541 ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc
     9601 ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt
     9661 tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg
     9721 tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg
     9781 cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta
     9841 tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg
     9901 agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca
     9961 agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag
    10021 ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg
    10081 cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg
    10141 ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat
    10201 ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact
    10261 cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg
    10321 gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca
    10381 gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat
    10441 cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga
    10501 gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataagggcag
    10561 aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt
    10621 taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat
    10681 cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc
    10741 ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc
    10801 tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata
    10861 ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt
    10921 tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct
    10981 gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa tgccgatttt
    11041 tcttcgccag aaacttttag ccgcaacgga ataagcagcg gctgagcgca aactggcgct
    11101 tcacccggcg tcagttgtac ctgacgcagc caacgttctg ccactggcaa ggctaaaagt
    11161 gaaatgttgc cgtcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga
    11221 ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttaatttt
    11281 tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag
    11341 cctggctgcg cattcggcca gggggcagcc ggacgactgc ctgatgctgg cgcaggagtg
    11401 tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc
    11461 acgcggtttt ccggaatgga acgcggtgca ggttggggtt catcgtccag cggtagcggc
    11521 gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga
    11581 cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgca cgttgacgtc cacctgatgc
    11641 ggatcgatct ccagatacag cacaaatgcc ggttgctgat cggcccccag tttgtcttcg
    11701 caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc gttcacgtag
    11761 caatactgaa tttctgccag tgcgggcgtg gtgtgatttg gatcggccac ccagccgcgt
    11821 agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcaagaaa agcggtgccg
    11881 caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc
    11941 acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg
    12001 cggatgatct catcaatgtg gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc
    12061 gccggggtgt tgtagaacag atccagcacc tccagcgtcg tccccacagg atgcgccgcc
    12121 ggttttaccg tcacgttcat atcgcgccct tcggcatagg cctgccaggc ttcctgctgt
    12181 tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca
    12241 ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg
    12301 gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga
    12361 atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca
    12421 tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac cacctcacct
    12481 gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag
    12541 tttggcagcg tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt tgccccctgc
    12601 ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca ggcctttgta aatggcttct
    12661 gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata
    12721 aaaccggttt cgaccagtac tgacgggata tccggcgaac gcagaacgcc gagactcgcg
    12781 tgttctggtc ggcgtttatg tatttcgcca atgcgttgca actgactgat catactggtc
    12841 gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt
    12901 aaataggggt cagactgact gttcgccagc acatcgcccg ccccacccag cagctccgac
    12961 tgtttctcgt gctgctccag ccagctggcc atttcgctgt tggcgcgacg gttagagagc
    13021 acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg aatcgacacg
    13081 aggaaattgg cgttttgctt acgtgccaca tcgctgcgcc ccatcaccga gataaagtaa
    13141 tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtgcgcaat
    13201 ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca
    13261 gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc
    13321 gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta
    13381 ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc
    13441 gtttcaacgc gtttcgcaac cacgggcggt ggaggaggcg gtggcggcac atcggcgtta
    13501 atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttaccg
    13561 ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga
    13621 atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat cacgcccgtt
    13681 tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg
    13741 ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag
    13801 agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg
    13861 atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat
    13921 tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg
    13981 tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg
    14041 cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg
    14101 gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata gggttcgacc
    14161 agcgtataag tggggctttt gacattaccc tgatgaccca gagcttgtaa aaaaccccgg
    14221 ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg
    14281 caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt
    14341 actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga
    14401 aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc
    14461 cgcaccgtgc gcgacacagc ccgcacaggc ggcatcatac ggcgacagtt tttgcccaag
    14521 caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt
    14581 tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt
    14641 cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact
    14701 ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat
    14761 cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca
    14821 caacatcggt ttgcgaaaat tctcaacttt ttgcagtgct tttttccccc actcttgctg
    14881 gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc
    14941 catcgtcagt tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg caatgttttc
    15001 actgcgggtc agtactcgga ctaaaccagc accagcacgc agcgccgctt cccccgtcat
    15061 acgaatagcc cccgccgtgc cgtgatcgcc accgataatc accagccgcc cgtgatcgcc
    15121 tttatgcgaa gtcgggcgac gcggtttcag ccagtgagaa agttgttctg ccgaaaaccg
    15181 ctgaattttc gtctcctgac ctgccagcca gctatccagc cccagtgagt caaaatgcag
    15241 ttgtcctgta acatcccgcg cttttccagt aagcaagcct ggtttcagcg caataaaagt
    15301 gatggtgtga tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag ccagcaggcc
    15361 ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg
    15421 gctaatggat tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat caacaatcag
    15481 atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca
    15541 tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggccaacaa
    15601 cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt
    15661 attaccatga ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac
    15721 ctggaatgcg gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc
    15781 tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact
    15841 tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt
    15901 agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga
    15961 taccgatctc agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg
    16021 cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgcccccatg agttattacc
    16081 aggtacgctg cgtgtaatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc
    16141 cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta
    16201 tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt
    16261 ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga
    16321 aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc
    16381 tttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga
    16441 ggaaggatgc ggtaaatgca tagcctgtat gacgatttgc ccgaccggtg ccatcgtcga
    16501 gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc
    16561 gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca
    16621 gcttatctgc ccgtggaatc gctattcgca actcactaca gaagacgatt tcagcccgcg
    16681 taagccgcta cacgcacagg aactcattga gttatttgcc tggagcgaag agaagttttt
    16741 aaaagtcacg gaaggatcgg cgattcgccg tataggtcac ctgcgctggc tgcgtaatat
    16801 cgccgtggca ttaggcaatg cgccctggga tgaaacgatt ctggcggcgc tggagagtcg
    16861 caaaggtgag cacccacttc ttgatgagca catagcgtgg gcgattgcgc agcaaatcga
    16921 gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt
    16981 gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa
    17041 aacgcattgc aattcaagac gcataaaatc gtcaagtgat cgactcgaca aaacggaatc
    17101 ttgattttta acttatttat caataggtta aaaaaatacc attcaccgtg cgaagtttct
    17161 ttgaaattaa gattacagcc tgaggatgtg gataactctg tttacaagag tttgtcagag
    17221 acaacaaaaa ccagcaataa cgcatcgcgt cgctgtggat attttattga gagaagaatt
    17281 tggagcggga aacgagactc gaactcgcga ccccgacctt ggcaaggtcg tgctctacca
    17341 actgagctat tcccgcttaa tcttcgtctt tcgaaccgct actgccttgg ctgccttcac
    17401 tcaccttagt cacatacttt tgtaagttcc caaggattcg ttcagttgcc gccttgtcgc
    17461 aattcgaaat actgcgattg ggtggtctgt gctttgcagc acttttcaaa ttttggagcg
    17521 ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta ccaactgagc
    17581 tattcccgcg tactacttaa ttttgctttc gtaattttcg catttctgcg tcgttacggg
    17641 aggcgcatta tacgagaaat ctttccctct gcaacccccc aaaaaagttt tttttgcgtt
    17701 tttatctcaa gtgctgaaat tatcagcaag acgttcaatt tagcgtcaaa atcacgccgc
    17761 cgcaagtgga ttccggcgct tacgtgacca ggaaaaattt tacagcttga taaaatgctc
    17821 gcggtagtaa gccagctccg ccaccgattc acggatatca tccattgcct gatgcgtccc
    17881 ctgcttggta aaaccatcca gaatttccgg cttccagcgg cgcgccagct ctttcagggt
    17941 gctgacatcg agataacggt agtggaagta ggcttccagc tccggcatgt atttaaacag
    18001 gaaacgacgg tcctgtccga tgctgttacc gcaaatcggc gattttcccg caggcaccca
    18061 ctgttttaag aattcgagcg ttgccagttc agcttcccga tcgcccatcg tgctcgcttt
    18121 cacgcgctct accagcccgc tggcggtatg ggtgcgcacg ttccagtcat ccatcagcgc
    18181 cagctgttca tcggactgat gtactgcaat ggtcggccct tctgccagaa tattcagatt
    18241 ggcatcggtc accagcgtgg caatctcaat aatgcgatcg cgctcgggat ccagaccggt
    18301 catctcaaga tcgatccaaa tcaggttgtt ttcattggca ctcatgctat tttccaccca
    18361 ttcacagtga ctatattgat ccacaattgc gtgtatcata gatgttttgc ccatcagggg
    18421 cgaccaggag tcagtacgat tgagtaaaaa taaactctcc aaaggccagc agcgccgcgt
    18481 gaacgccaat caccagcgtc gtcttaaaac gtctaaggag aagcccgact acgacgacaa
    18541 tctgtttggc gagcctgatg aaggtatcgt catcagccgc tttggtatgc acgctgatgt
    18601 ggaatccgcc gatggcgacg ttcaccgctg caatattcgc cgtaccatcc gttcgctggt
    18661 aaccggcgac cgcgtagtct ggcgtccggg taaaccggcg gcggaaggcg taaatgtcaa
    18721 agggatcgtg gaagcggtgc atgagcgtac ctcggtgttg acgcgtccgg atttctacga
    18781 cggcgtaaaa cctattgccg ccaacatcga ccagattgtc attgtctccg ccattttgcc
    18841 ggagctgtcg ctcaatatta tcgaccgtta cctagtggcc tgcgaaacct tgcagattga
    18901 gccgattatt gtgctcaaca agatagacct gctggacgac gaaggcatgg cgttcgtcaa
    18961 cgagcagatg gatatctacc gcaatatcgg ttatcgcgta ttgatggttt ccagccatac
    19021 tcaggatggg ctaaaaccgc tggaagaggc gttgaccggg cgcatcagca tttttgccgg
    19081 gcagtctggc gtcggcaaat ccagcctgct gaacgcgttg ctggggctgc aaaaagagat
    19141 cctgaccaac gatgtctctg acaactcggg gcttggtcag cacaccacga ccgccgctcg
    19201 gctgtatcac ttcccgcacg gcggtgatgt gattgattcc cctggtgtgc gtgagttcgg
    19261 cctctggcac ctggagccgg aacaaatcac tcagggcttt gtcgaattcc atgactattt
    19321 aggtctgtgt aaatatcgcg attgcaaaca cgataccgat ccgggctgcg ctatccggga
    19381 agcggttgag gaagggaaaa tcgcggaaac ccgtttcgaa aactatcacc gtattctgga
    19441 aagcatggcg caggtaaaaa cgcgtaaaaa cttttctgat acggatgact gacaattaag
    19501 ctaaccgtcg ttagaatcgt cccctttttt tcaggatccg gcatgtaggc cgaatcagga
    19561 acgacaaaac aatggcctgg aggctacctt gttaaattca tttaaacttt cgctacagta
    19621 cattctgccg aaactatggc ttactcgcct ggcgggttgg ggcgcaagca agcgggcagg
    19681 atggttgaca aaactggtta tcgatctgtt cgttaaatac tacaaggtcg acatgaaaga
    19741 ggcgcaaaag ccggacaccg ccagctaccg cacctttaac gaattctttg tccgtccgct
    19801 gcgtgacgaa gtacgcccaa tcgataccga tccgaatgta ctggtcatgc ctgccgatgg
    19861 cgttatcagc cagttgggta aaatcgaaga agataaaatc ctgcaagcca aaggccacaa
    19921 ctacagcctc gaagccctgc tggcaggcaa ctatctgatg gcggacctgt tccgcaacgg
    19981 tacgtttgtg accacttacc tctccccgcg tgactaccac cgcgtacaca tgccgtgcaa
    20041 cggtattctg cgtgagatga tctacgtgcc gggcgatctc ttctccgtta accatctcac
    20101 ggctcagaac gtgccgaatc tgtttgcccg taacgaacgc gtgatttgcc ttttcgatac
    20161 cgaatttggc ccaatggcgc agattctggt cggagcgacg attgttggca gcattgagac
    20221 ggtctgggcg ggcaccatta cgccgccgcg cgaaggtatc atcaagcgct ggacctggcc
    20281 tgccggggaa aacgacggtt ctgtggcact gctgaaaggc caggaaatgg gtcgctttaa
    20341 actcggttcc accgttatca acctgtttgc accgggtaaa gtgaatctgg ttgagcaact
    20401 ggaaagcctg tctgtcacga aaatttgcca gccgctggca gtatctaccg aaacctttgt
    20461 tacgccagac gctgaacctg ccccgcttcc tgctgaagag atcgaagcag aacacgacgc
    20521 cagcccattg gttgacgaca aaaaagacca ggtctaatcc atcaaaggaa acgctgacgt
    20581 gcgcctgatt atcacttttc tgatggcctg gtgcctcagt tggggggcgt acgccgcgac
    20641 ggcccccgat agcaaacaaa tcactcagga actggagcag gcaaaagcgg cgaaacccgc
    20701 acagccggaa gtcgtagagg cgcttcagtc tgccttaaat gcgcttgagg aacgaaaagg
    20761 ttcccttgag cgcatcaaac aatatcagca agttatcgat aattatccga aactctccgc
    20821 tactctgcgc gcacaattaa acaacatgcg tgacaagccg cgcagcgtgt cgccgggaat
    20881 gtctaccgac gcgctgaatc aggaaattct ccaggtcagc agccagttgc tggataaaag
    20941 ccgtcaggcc cagcaagagc aggagcgcgc ccgcgagatt gccgattcgc tgaatcaact
    21001 gccgcaacag caaaccgacg cccgccgtca gttaaatgag atcgagcgcc gcctgggaac
    21061 gcttaccggc aatactccgc tcaatcaggc acaaaatttc gcgttgcagt ctgactctgc
    21121 acgtcttaag gcgctcgttg atgaactgga gctggcgcag ctgtctgcca ataaccgcca
    21181 agaattagcg cgcttacgct cagagcttgc ggaaaaagag agccagcaac tggatgcgta
    21241 tttgcaggcc ttacgtaatc agttgaatag ccaacgtcag cttgaggcgg agcgggcgct
    21301 ggaaagtacc gaattactgg cagaaaacag tgccgatttg ccgaaagata tcgtcgcgca
    21361 attcaaaatt aaccgcgaac tatcggcagc gctgaatcaa caggcgcagc ggatggatct
    21421 cgttgcctcg caacagcgtc aggctgccag ccagacgtta caggtccggc aggcgttgaa
    21481 tacgctgcgt gaacagtcgc aatggctggg atcgtccaat ctgctcggtg aagcgttgcg
    21541 agcgcaggtg gcacggctac cagaaatgcc aaaaccacaa cagcttgata ccgaaatggc
    21601 gcagttgcgt gtgcaacggt tacgttatga ggatctgctt aataaacagc cgctgctacg
    21661 gcaaatccat caggccgacg gtcagccgct gaccgccgag caaaaccgta ttctggaagc
    21721 acaactgcgc actcagcgtg agttgctgaa ctcattgttg cagggtggcg acacgctact
    21781 attggaactg accaagctga aagtctccaa cgggcaactg gaggatgcgc tgaaagaggt
    21841 gaacgaagca acgcaccgct atctgttctg gacctctgac gtgcgtccga tgaccatcgc
    21901 ctggccgctg gaaatcgccc aggatctgcg tcgtctcatt tcgctggaca ccttcagtca
    21961 gttgggcaaa gccagtgtga tgatgctgac cagcaaagag acaattttgc cgctgtttgg
    22021 cgcgttgatt ctggtcggtt gcagtattta ctcgcgccgc tatttcaccc gttttcttga
    22081 acgttcggcg gcgaaagttg gcaaagtgac tcaggatcac ttctggctga cgttgcgcac
    22141 tcttttctgg tcgattctcg tcgcgtcacc gttaccggtg ctgtggatga cgctgggtta
    22201 cggcttgcgc gaggcgtggc cttatccgct ggcggtcgcg attggcgatg gtgtaacggc
    22261 caccgtgccg ctgctgtggg tagtgatgat ttgcgccacc tttgcccgcc cgaacggctt
    22321 gtttatcgct cattttggct ggccgcgcga acgcgtatct cgtgggatgc gctactacct
    22381 gatgagcatc gggcttattg tgccgctgat tatggcgctg atgatgttcg ataacctcga
    22441 cgaccgtgaa ttctccggtt cgctgggaag gctttgcttt atcctcattt gcggtgcgct
    22501 ggcggtggtc accctcagcc tgaaaaaggc cgggattccg ctgtatctca acaaagaagg
    22561 cagcggcgac aacattacca accatatgct gtggaacatg atgattggcg cgccgttggt
    22621 tgccattctg gcgtcggctg tgggttatct ggcaacggca caggcgctgt tagcgaggct
    22681 tgaaacctcg gttgccatct ggttcctgct actggtggtt tatcacgtta tccgccgctg
    22741 gatgctgatc cagcgccgca ggctggcgtt tgaccgggcg aagcatcgcc gggcagagat
    22801 gttagcgcaa cgtgcgcgtg gcgaagagga agcgcatcat cacagtagcc cggaaggagc
    22861 aattgaagtc gatgaaagcg aagtcgatct cgatgccatc agtgcgcaat ccttgcggct
    22921 ggtgcgctca attttgatgt tgatcgccct gctttctgtc attgtgctgt ggtcagaaat
    22981 ccattccgct ttcggcttcc tcgaaaatat ttcgctgtgg gatgtcacct ccacggtaca
    23041 gggcgtagaa agtctggagc caattaccct cggtgcggtg ctgattgcca ttctggtgtt
    23101 tatcatcacc acgcagctgg tgcgcaacct gcccgcgctg ctggaactgg cgattttgca
    23161 gcacctggat ttaacgccgg gtacgggcta cgccatcacc accatcacca aatatctgct
    23221 gatgctgatt ggcgggctgg tcggcttctc gatgattggt attgagtggt cgaaattgca
    23281 gtggctggtc gccgcgctcg gtgttggtct cggttttggt ttgcaggaaa ttttcgccaa
    23341 ctttatctct ggtctgatta tcctgttcga aaaaccgatt cgcattggcg atacggtgac
    23401 aattcgcgat ctcaccggta gcgtgacgaa aattaacacc cgcgccacca ccatcagcga
    23461 ctgggaccgt aaagagataa tcgtgccgaa caaggcgttt attaccgagc agtttatcaa
    23521 ctggtcgctc tctgactcgg tcacgcgcgt agtgttgacg attccggccc ctgctgatgc
    23581 caacagtgaa gaagtgacgg aaatcctgct caccgcagcg cgtcgctgct cgctggtgat
    23641 cgacaacccg gcaccggaag tcttcctggt ggatctgcaa caagggattc agattttcga
    23701 gctgcgtatt tatgccgctg agatgggtca ccgtatgccg ctacgccatg agatccacca
    23761 gctgattctg gctggcttcc atgcccacgg tatcgatatg ccattcccgc ccttccagat
    23821 gcgtctggaa agcctcaacg gtaaacaaac ggggagaacg ctgacgtctg cgggcaaagg
    23881 tcgtcaggcg ggaagtttgt aatgcatgaa gctgccaaat gatgtcaacg tggctcattt
    23941 agcagcttaa catcatcttc agttatcacc ggccctggag agtatcatcc atacgcatta
    24001 cttcctgaca actcatcgcc ggagagacga tgagttgcag gatttcatat gatcagtcga
    24061 gagctaactc aggtttattc gccatgcgac tgcaatagtt ctggtaaatc gccatcgcta
    24121 acagcgagaa gaagacaggg ccgccaatca tccacaatgt gctgtcccag tctccggctt
    24181 caaccacagg ctgaataatg gtgaagacgt tcgcaaatgt caccaccagt acgacaacca
    24241 ccatcgcaat cattgccgac atacgcgttt tgaaaatcac aaatggtctg tcgagatcct
    24301 gacgtgcttt aaagaatggg aaagccagcg cgaggaacag gtaaggaagc gtcatagaca
    24361 cgttcgccat cagcgtcagc ttgttaaaga acgcggatgc ggtaccgcca ccaaacgaaa
    24421 ccagcaggat gaagacagta accaacccgc actgcatcca catggcgata gacggcatcc
    24481 ccatcgcatt caggcgcgtc atcggttccg gccacaacgc tttcggcgtc ccctggatga
    24541 tggctttcag cggtgaatag caaagcgtaa agaacgcacc ggtataagcg aggaacatcg
    24601 aaagtccggt aatacgcgca aaccatacgc ccagcgacaa tgacgcttca ggtgacaaat
    24661 gcagtgcgtt acccagcgtc accccgaggc tcttcatcag cacataggta atattgccaa
    24721 ggttaacgga accattactt aatacctgct gccagtttgt gctgacgccc cataaaaata
    24781 ttgccagcga ataaccgatt gaaataacaa tagcggcaaa aacaatacct ttggcaaagt
    24841 tcttttctgg attttccgtt ttatcgacca gaccaccaac cgcttcaatt ccgccatagg
    24901 caaaaatagc aaataccaca aacgatagca ttgccagacc ggactgataa cccgggttcg
    24961 gtgatgcaag gaaattaata tcctgcgcga aatgcccacc atttaataac aaaatagtaa
    25021 tgcttactaa cagcaatact aaattcagac acattactgc aataccgccc accgcagtaa
    25081 tgcgggcaat tttattaatc ccctttgaag caacgacggt gaccagaatc atccatgcta
    25141 ctgccagcag accaaccacc tgcgtaggct ccagtccggc aatacgccag tgctgggtca
    25201 tgtcgctacc atagaggaat gttgagaacg gtacccaaac tttcgcggag gtactcacca
    25261 tccagatgat ataagaggaa aaccacataa acgtaccaat gaaggcaaaa cgtggtccga
    25321 cactattatt catccaggaa tagataccac cttcttcttt gcgataagca gctcccattt
    25381 ca
//
LOCUS       DAJTYZ010000053        25105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000053 DAJTYZ010000000
VERSION     DAJTYZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.053"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            196..384
                     /locus_tag="OO596_002075"
     CDS             196..384
                     /locus_tag="OO596_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6717935.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="OO596_002076"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="OO596_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6717936.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="OO596_002077"
     CDS             1145..1309
                     /locus_tag="OO596_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6717937.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1502..3763
                     /gene="bcsA"
                     /locus_tag="OO596_002078"
     CDS             1502..3763
                     /gene="bcsA"
                     /locus_tag="OO596_002078"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR6717938.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3774..6112
                     /gene="bcsB"
                     /locus_tag="OO596_002079"
                     /pseudo
     CDS             3774..6112
                     /gene="bcsB"
                     /locus_tag="OO596_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            6119..7225
                     /gene="bcsZ"
                     /locus_tag="OO596_002080"
     CDS             6119..7225
                     /gene="bcsZ"
                     /locus_tag="OO596_002080"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6717939.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7207..10699
                     /gene="bcsC"
                     /locus_tag="OO596_002081"
                     /pseudo
     CDS             7207..10699
                     /gene="bcsC"
                     /locus_tag="OO596_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10781..12769
                     /gene="hmsP"
                     /locus_tag="OO596_002082"
     CDS             10781..12769
                     /gene="hmsP"
                     /locus_tag="OO596_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6717940.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12952..14238
                     /gene="dctA"
                     /locus_tag="OO596_002083"
     CDS             12952..14238
                     /gene="dctA"
                     /locus_tag="OO596_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6717941.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14458..15954
                     /locus_tag="OO596_002084"
     CDS             14458..15954
                     /locus_tag="OO596_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6717942.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16050..16979)
                     /gene="kdgK"
                     /locus_tag="OO596_002085"
     CDS             complement(16050..16979)
                     /gene="kdgK"
                     /locus_tag="OO596_002085"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6717943.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17211..17978
                     /gene="pdeH"
                     /locus_tag="OO596_002086"
     CDS             17211..17978
                     /gene="pdeH"
                     /locus_tag="OO596_002086"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6717944.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18048..20108
                     /locus_tag="OO596_002087"
     CDS             18048..20108
                     /locus_tag="OO596_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6717945.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20290..21612)
                     /locus_tag="OO596_002088"
     CDS             complement(20290..21612)
                     /locus_tag="OO596_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6717946.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22023..23036)
                     /gene="yhjD"
                     /locus_tag="OO596_002089"
     CDS             complement(22023..23036)
                     /gene="yhjD"
                     /locus_tag="OO596_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6717947.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23085..23984)
                     /locus_tag="OO596_002090"
     CDS             complement(23085..23984)
                     /locus_tag="OO596_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6717948.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24504..>25105
                     /locus_tag="OO596_002091"
     CDS             24504..>25105
                     /locus_tag="OO596_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6717949.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5895 a         6365 c         6991 g         5854 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tgaggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcggctgcc
     4201 agtgaccaaa gaacagttgg gtaaaaaaac gctggcgcaa atgcccatta acccactgtt
     4261 tattaccgac ttcaaccgtg tgcggctgga gtttgtcggc cattatcagg acgtgtgcga
     4321 aaacccggcc agcaccacgc tttggctgga tgttgggcgg agcagtggac tggatctgac
     4381 ctatcagacc ctgaatgtga agagtgacct gtcacacttc ccggtgccat tctttgaccc
     4441 gcgcgataac cgtactaaca ccttgccgat ggtctttgcg ggtgcgccgg atgttgggct
     4501 gcaacaagca tctgccattg tcgcctcgtg gtttggttcg cgttctggct ggcgtgggca
     4561 gaacttcccg gtgctctata accaattgcc ggatcgcaat gccattgtct ttgcaaccaa
     4621 cgacaaacgg ccggacttcc tgcgcgatca tccggcggta aaagccccgg tgattgagat
     4681 gattaaccat ccgcagaatc cttacgtcaa actgctggtg gtgtttggtc gtgacgacaa
     4741 agacctgttg caggcagcga aaggtatcgc tcagggtaac attctgttcc gtggtgaaag
     4801 cgtggtagtg aatgaagtga aaccgctgct accgcgtaag ccgtacgatg cgccgaactg
     4861 ggtacgtacc gatcgtccgg tcacatttgg cgaactgaaa acctatgaag aacagttaca
     4921 atccagcggt cttgagccag cagcgattaa cgtttcgcta aacctgccgc cggatctcta
     4981 cctgatgcgc agtaccggca ttgatatgga tattaattac cgctacacca tgccgccggt
     5041 gaaagacagt tcgcggatgg atatcagcct gaataaccag ttcctgcaat ccttcaacct
     5101 gagcagtaaa caggaggcga accgcctgct gctgcggatt ccggtattac aaggtttgct
     5161 ggatggcaaa acggatgtct ctattccggc gctgaaactg ggcgcgacca accagctgcg
     5221 cttcgacttt gagtatatga acccgatgcc gggcggttcg gtggataact gtattacctt
     5281 ccagccggtg cagaatcatg tggtgattgg tgacgattcc accatcgact tctcgaagta
     5341 ttaccacttc atcccgatgc cggatctacg cgcctttgct aacgcgggct tcccgttcag
     5401 ccggatggcg gatctgtcgc aaaccatcac cgtgatgccg aaaacgccta acgaagcaca
     5461 gatggaaacg ttgctgaata ctgttggttt tatcggcgca cagacgggct tcccggcgat
     5521 taatctgacg gtgaccgatg atggcagcac cattcagggc aaagatgccg acatcatgat
     5581 catcggtggt atcccggaca aattaaaaga cgataagcag atcgacctgt tggtgcaggc
     5641 gaccgaaagc tgggtgaaaa caccgatgcg ccagaccccg ttccccggca ttgtgccgga
     5701 cgagagcgat cgtgcggcag aaacccagtc aacgctgacc tcttccggtg cgatggcggc
     5761 ggtgattggc ttccagtcgc cgtataacga ccagcgcagc gtgattgcgc tgctggcaga
     5821 tagcccacgc ggttatgaaa tgcttaacga tgcggtgaac gatagcggca aacgcgccac
     5881 catgttcggt tcggtcgcgg tgatccgcga gtccggtatc aacagcctac gtgttggcga
     5941 cgtttattac gtaggtcatc tgccgtggtt cgagcgcttg tggtatgcgc tggcaaacca
     6001 tccgattctg ctggcggtgc tggcggcaat cagtgtgata ttgctggcat gggtactgtg
     6061 gcgtctgctg cgaattatta gtcgtcgtcg tcttaacccg gataacgagt aattgaagat
     6121 gaatgtgttg cgtagtggaa tcgtgacgat gctgctgttg gctgccttta gtgttcaggc
     6181 agcctgtacc tggcctgcct gggagcagtt taaaaaggat tacatcagtc aggaagggcg
     6241 cgtcatcgcc cccagcgacg cgcgcaaaat caccacctcc gaagggcaaa gttacggtat
     6301 gttctttgcc ctggcggcta acgaccgtgt agctttcgat aatattctcg actggacgca
     6361 gaacaatctc gctcagggct ctttaaaaga acgtttgccc gcctggctgt ggggcaagaa
     6421 agagaacagt aagtgggaag tgctggacag caattcggcc tccgatggtg atgtctggat
     6481 ggcctggtcg ttgctggagg cggggcgttt gtggaaagag cagcgttata ccgacatcgg
     6541 cagcgcgttg ctaaaacgta tcgcgcggga ggaagtggtg acggtgcctg ggctgggttc
     6601 catgttgtta ccgggcaaag tgggttttgc tgaggataac agctggcgtt ttaaccccag
     6661 ctacctgccg ccgacgctgg cgcagtattt cacccgcttt ggcgcgccgt ggaccacgct
     6721 gcgcgaaacc aatcaacgtt tattgctgga aaccgccccg aaaggttttt cgccagactg
     6781 ggtgcgctat gagaaagaca aaggctggca gctaaaagcc gaaaaaacat tgatcagcag
     6841 ctacgacgct atccgcgttt acatgtgggt aggcatgatg cctgacagcg atccgcaaaa
     6901 agcgcggatg ctcaaccggt ttaaaccgat ggcgacattc actgagaaaa acggttatcc
     6961 gccggaaaaa gtggatgtgg ctacggggaa agcgcagggt aaaggaccga tcggtttttc
     7021 tgccgccatg ctgccctttt tacaaaaccg cgatgcgcag gccgttcagc gccagcgcgt
     7081 ggccgataac tttcccggca gcgatgccta ttacaactat gtgctgaccc tgtttggaca
     7141 aggctgggat caacaccgtt tccgcttctc gacaaaaggt gagttattac ctgactgggg
     7201 ccaggaatgc gcaaattcac actaaacata ttcacgcttt ccctcggtct ggccgtcatg
     7261 ccgatggtcg aggcagcacc aaccgctcag caacagttgc tggagcaagt tcggttaggc
     7321 gaagcgaccc atcgtgaaga tctggtgcaa cagtcgttat atcggctgga acttattgat
     7381 ccgaataacc cggacgtcgt tgccgcccgt ttccgttctt tgttacgtca gggcgatatt
     7441 gatggcgcgc aaaaacagct cgatcggctg tcgcagttag cgccgagttc aaatgcgtat
     7501 aaatcgtcgc ggactacgat gctactttcc acgccggatg gtcgtcaggc actgcaacag
     7561 gcacgattgc aggcgacgac cggtcatgca gaagaagctg tggcgagtta caacaaacta
     7621 ttcaacggtg cgccgccgga aggtgacatt gctgtcgagt actggagtac ggtggcgaaa
     7681 attccggctc gccgtggcga agcgattaat cagttaaaac gcatcaatgc ggatgcaccg
     7741 ggcaatacgg gcctgcaaaa caatctggcg ctattgctgt ttagtagcga tcgccgtgac
     7801 gaaggttttg ccgtcctgga acagatggca aaatcgaacg ccgggcgcga aggggcctct
     7861 aaaatctggt acgggcagat taaagacatg cccgtcagtg atgccagtgt gtcggcgctg
     7921 aaaaaatatc tctcgatctt tagtgatggc gatagcgtgg cggctgcgca atcgcaactg
     7981 gcagaacagc aaaaacagct ggccgatcct gctttccgcg ctcgtgcgca aggtttagcg
     8041 gcggtggact ctggtatggc gggtaaagcc attcccgaac tacaacaggc ggtgcgggcg
     8101 aacccgaaag acagtgaagc tctgggggcg ctgggccagg cgtattctca gaaaggcgat
     8161 cgcgccaatg cagtggcgaa tctggaaaaa gccctcgcac tggacccgca cagcagcaac
     8221 aacgacaaat ggaacagtct gctgaaagta aaccgctact ggctggcgat ccagcagggc
     8281 gatgctgcgc tgaaagccaa taatcctgac cgggcagaac gcctgttcca gcaggcgcgt
     8341 aatgtcgata acaccgacag ttatgcagtg ctggggctgg gcgatgtggc gatggcgcga
     8401 aaagattatc ccgccgccga acgttattat cagcagacct tgcgtatgga cagcggcaac
     8461 actaacgccg tgcgcgggct ggcaaatatt taccgccagc aatcgccaga aaaagctgaa
     8521 gcgtttatcg cctcgctctc tgccagtcag cggcgtagca ttgatgatat cgaacgcagc
     8581 ctgcaaaacg accgtctggc acagcaggca gaggcactgg aaaaccaggg caaatgggcg
     8641 caggcggcag cacttcagcg gcaacgactg gcgctggacc ccggcagcgt atggattact
     8701 taccgacttt cgcaggatct ctggcaggcc ggacaacgca gccaggccga tacgttaatg
     8761 cgcaatctgg cgcagcagaa gtcgaacgac ccagagcagg tttacgctta cgggctgtac
     8821 ctacgcttac gggctgtacc tctctggtca tgaccaggac agagcggcgc tggcgcatat
     8881 caacagcctg ccgcgtgcgc agtggaacag caatattcag gagctggtta atcgactgca
     8941 aagcgatcag gtgctggaaa ccgctaaccg cctgcgagaa agcggcaaag aggcagaagc
     9001 ggaagcgatg ctgcgccagc aaccaccttc cacgcgtatt gacctcacgc tggctgactg
     9061 ggcgcaacaa cgacgtgatt acaccgccgc ccgcgctgca tatcagaatg tcctgacgcg
     9121 ggagccagct aacgccgacg ccattcttgg tctgacggaa gtggatattg ctgccggtga
     9181 caaagcggcg gcacgtagcc agctggcgaa actgcccgct accgataacg cctcgctgaa
     9241 cacacagcgg cgcgtggcgc tggcacaggc gcagcttggc gataccgcag cagcgcagcg
     9301 gacgtttaat aagttgatcc cgcaggcaaa atctcagcca ccgtcgatgg aaagcgcgat
     9361 ggtgctgcgt gatggtgcga agtttgaagc gcaggcgggc gatccaacgc aggcgctgga
     9421 aacctacaaa gacgccatgg tcgcatccgg tgtgactacg acgcgtccgc aggataacga
     9481 cacctttacc cgactgaccc gtaacgacga gaaagatgac tggctgaaac gtggcgtgcg
     9541 cagcgatgcg gcggacctct atcgccagca ggatcttaac gtcacccttg agcacgatta
     9601 ctggggttcg agcggcaccg gtggttactc cgatctgaaa gcgcacacta ccatgttgca
     9661 ggtggatgcg ccgtattctg acgggcggat gttctttcgc agtgatttcg tcaatatgaa
     9721 cgtcggcagt ttctccacta atgccgatgg caaatgggat gacaactggg gcacctgtac
     9781 attacaggac tgtagcggca accgcagcca gtcggattcc ggtgccagcg tggcggtcgg
     9841 ctggcgaaat gacgtctgga gctgggatat cggtaccacg ccgatgggct tcaacgtggt
     9901 ggatgtggtc ggcggcatca gttacagcga tgatatcggg ccgctgggtt acaccgttaa
     9961 cgcccaccgt cggcccatct ccagttcttt gctggccttt ggtgggcaaa aagactcccc
    10021 gagcaatacc gggaaaaaat ggggtggcgt acgtgccgac ggtgtggggc taagtctgag
    10081 ctacgataaa ggtgaagcaa acggcgtctg ggcatcgctt agtggcgacc agttaaccgg
    10141 taaaaatgtc gaagataact ggcgcgtgcg ctggatgacg ggctattact ataaggtcat
    10201 taaccagaac aatcgccgcg tcacaatcgg cctgaacaac atgatctggc attacgacaa
    10261 agatctgagt ggctactcac tcggtcaggg cggttactac agtccgcagg aatacctgtc
    10321 gtttgccata ccggtgatgt ggcgggagcg cacggaaaac tggtcgtggg agctgggggc
    10381 gtctggctcg tggtcgcatt cacgcaccaa aaccatgccg cgttatccgc tgatgaatct
    10441 gatcccgacc gactggcagg aagaagctgc gcggcaatcc aacgatggcg gcagcagtca
    10501 gggcttcggc tacacggcgc gggcattact tgaacgacgt gttacttcca actggtttgt
    10561 tggcacggca attgatatcc agcaggcgaa agattacgca cccagccatt tcctgctcta
    10621 cgtacgttat tccgccgccg gatggcaggg tgacatggat ttaccgccgc agccgctgat
    10681 accttacgcc gactggtaag ttttcagata gcgcctctct taatgccgct gcgatcgggt
    10741 atactcgggc ggcaatctgg gatttccggg gggagacaat ttgcgcgtaa gtcgctcgtt
    10801 aacaatcaag cagatggcaa tggtggcagc cgttgtcctg gtgttcgttt ttattttttg
    10861 caccgttttg ctgttccatc tggtccagca gaatcgctat aacacggcta cgcaactgga
    10921 aagcattgct cgctctgtcc gcgaaccctt atcttcagct attttgaaag gcgatattcc
    10981 cgaagcggaa gctattcttg ccagcattaa accggcaggc gtggtcagcc gtgccgatgt
    11041 agtgctgcct aaccagttcc aggcgctgcg taaaagtttt attccagagc gcccggtgcc
    11101 gataatggtt actcgcctgt ttgagctacc ggttcaaatc tcgctgggcg tttactcgct
    11161 cgaacgtccg gcaaacccgc agccaattgc ctatctggta ctacaggcgg attccttccg
    11221 tatgtataag ttcgtgatga gcaccctctc aacgttagtg accatttact tacttttgtc
    11281 gcttatcctg accgtcgcca tcagctggtg cattaaccgc ctgattttgc atccgttacg
    11341 caatattgct cgcgaactta acgccatccc agcccaggag cttgttggtc accaactggc
    11401 attaccgcgt ctgcatcagg acgatgaaat cggtatgttg gtgcgcagtt acaacctcaa
    11461 ccagcaattg ctgcagcgcc attatgaaga acagaacgaa aatgcgatgc gcttcccggt
    11521 gtcggatttg ccgaacaaag ccttgctgat ggagatgctg gagcaggttg tcgcgcgtaa
    11581 acaaaccacc gcgctgatga tcatcacctg tgaaaccctg cgtgatactg cgggcgtgct
    11641 gaaagaggcg caacgagaaa ttctgctgct gacgctggtg gaaaaactca aatcggtact
    11701 gtcgccacgt atgatcctcg cgcagattag cggttatgac tttgctgtca ttgccaacgg
    11761 tgtacaggaa ccgtggcacg caatcacctt aggtcagcaa gtgctcacca tcatgagcga
    11821 gcgcctgccg attgaacgta ttcaactccg tccgcactgt agcattggcg tggcgatgtt
    11881 ctacggcgat ctcaccgccg aacagcttta cagtcgcgct atttctgcgg catttaccgc
    11941 tcgccataaa ggcaagaatc agattcagtt ctttgatccg cagcagatgg aagccgccca
    12001 gaagcggttg acggaagaga gcgatatcct taatgcactg gaaaatcatc agtttgctat
    12061 ttggttacag ccacaggtcg agatgaccag cggtaaactg gtcagtgcgg aagtgttact
    12121 gcgtatccag caaccggatg gcagttggga cctgccggat ggcttaatcg atcgcattga
    12181 gtgctgtggg ctgatggtta ccgtcggtca ctgggtgctg gaagagtcct gtcgattgct
    12241 tgcagcctgg caagagcgcg gcattatgct gcccttgtcg gtaaacctct ctgcgctgca
    12301 actgatgcac ccaaatatgg tggcggatat gctggaactg ttaacccgct atcgcattca
    12361 gccgggaaca ctgattctgg aagtgacaga aagccgacgt attgacgacc ctcatgctgc
    12421 ggtggcaatc ctccgtccgc tgcgcaatgc cggagttcgg gtggcgctgg atgatttcgg
    12481 catgggctac gcagggctgc gtcagctgca gcatatgaaa tcgttgccaa tcgacgtact
    12541 gaaaatcgac aaaatgtttg ttgaaggctt gccggaagat agcagcatga ttgctgcaat
    12601 tatcatgctg gcgcagagcc tgaacttaca aatgattgcc gaaggcgtgg agactgaagc
    12661 acaacgcgac tggctggcaa aagcgggcgt tggtattgcc cagggcttcc tttttgctca
    12721 cccactccct attgaaatct tcgaagagag ttacctggaa gaaaagtagc taccccaaac
    12781 tgattacaaa actttaaaaa gtgctggttt gtgcgagcca gctcaaactt tttaaccttt
    12841 ttgtttcaat tatgatccag gtacatttct gtgatgttgt ctgggtgtta ttttaaggcc
    12901 gcaggtaccc cataacctta caagacctgt ggttttacta aaggacaccc tatgaaaacc
    12961 tctctgttta aaagccttta ctttcaggtc ctgacagcga tagccattgg tattctcctt
    13021 ggccatttct atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg cttcgttaag
    13081 ctcattaaga tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg cattgcgggc
    13141 atggaaagca tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta ctttgaaatt
    13201 gtcagtacca tcgcgctgat tattggtctt atcatcgtta acgtcgtgca gcctggtgcc
    13261 ggaatgaacg tcgatccggc aacgcttgat gcgaaagcgg tagcggttta cgccgatcag
    13321 gcgaaagacc agggcattgt cgccttcatt atggatgtca tcccggcgag cgtcattggc
    13381 gcatttgcca gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt tggttttgcg
    13441 ctccaccgtc tgggcagcaa aggccaactg atttttaacg tcatcgaaag tttctcgcag
    13501 gtcatcttcg gcatcatcaa tatgatcatg cgtctggcac ctattggtgc gttcggggca
    13561 atggcgttta ccatcggtaa atacggcgtc ggcacactgg tgcaactggg gcagctgatt
    13621 atctgtttct atattacctg tatcctgttt gtggtgctgg tattgggttc aatcgctaaa
    13681 gcgactggtt tcagtatctt caaatttatc cgctacatcc gtgaagaact gctgattgta
    13741 ctggggactt catcttccga gtcggcgctg ccgcgtatgc tcgacaagat ggagaaactc
    13801 ggctgccgca aatcggtggt ggggctggtc atcccgacag gctactcgtt taaccttgat
    13861 ggcacatcga tatacctgac aatggcggcg gtgtttatcg cccaggccac taacagtcag
    13921 atggatatcg tccaccaaat cacgctgtta atcgtgttgc tgctttcttc taaaggggcg
    13981 gcaggggtaa cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc ggtgggccat
    14041 ttgccggtag cgggtctggc gctgatcctc ggtatcgacc gctttatgtc agaagctcgt
    14101 gcgctgacta acctggtcgg taacggcgta gcgaccattg tcgttgctaa gtgggtgaaa
    14161 gaactggacc acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga tggcaaaacg
    14221 cacgaattat cctcttaatc tcaccactta tgcccgcact cccttccccg agtgcgggtg
    14281 tttgcgcata attgacccct gtgcctgaac ttaccacctg ggtgagttca ggtttatttc
    14341 acttcttccg tgacattttc atgttcttgc ggtctaacac gaagtgtttt taacgtcatt
    14401 tagactgact gacaacgggc agttttttat taccaggata gttgatcagg ggttcacatg
    14461 cagggcacaa aaattcgact tttagcgggc ggtttgctga tgatggccac tgctggctat
    14521 gtgcaggcag atgcgctcca gcctgatcca gcatggcaac aggggacgct ttccaacggt
    14581 ttacagtggc aagtgctgac caccccccag cgtcccagcg atcgtgttga aattcgcctg
    14641 ctggttaata tcggttcgct cgccgaaagt acacaacaga gcggttacag tcacgccatc
    14701 cctcgtattg cgctaacgca aagcggtggc cttgacgcag cacaggcgcg ttcattgtgg
    14761 cagcagggga tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta tgacaccacg
    14821 ctgtttaatc tgagtttgcc caataaccgt aacgatttgc tgaaagaagc gctctcttat
    14881 ctggcaaatg ccactggcaa attgaccatc acaccagaaa ccatcaacca cgcgctgcaa
    14941 agtcaggaca tggtggcaac ctggcctgcc gatactaaag agggctggtg gcgctatcgt
    15001 ctgaaagggt caaccttgtt aggtcacgat cctgccgatc cgctgaaaca acccgttgaa
    15061 gcggaaaaaa ttaaagattt ctatcagaaa tggtacaccc cggatgcaat gacgctactg
    15121 gtggtgggaa acgtggatgc gcgctcggtt gtcgaccaaa tcaacaaaac gtttggcgaa
    15181 ctgaaaggca aacgtgaaac gccagctccg gtgccgacgc tttctccgct gcgtgcggaa
    15241 gcggtgagta ttatgactga cgcggtgcgt caggaccggt tatctatcat gtgggatacg
    15301 ccgtggcagc cgattcgtga atcagccgca ctgctgcgct actggcgtgc ggacctggcc
    15361 cgcgaggcgc tgttctggca tgttcagcaa gcgttaagcg ccagtaacag caaagacatc
    15421 ggtcttggat ttgactgccg tgtgctgtat ctgcgtgcgc agtgtgccat caacatcgaa
    15481 tcaccaaacg acaagctgaa cagcaacctt aatctggtgg cgcgtgaact ggcgaaggtt
    15541 cgcgataaag gtctgccgga agaagagttc aatgcgttag tggcgcaaaa gaaactggag
    15601 ctgcagaaac tgtttgccgc ctatgcgcgg gctgataccg atattctgat gggtcagcgg
    15661 atgcgttcgc tgcaaaatca ggtcgtcgat atcgcgccgg agcagtatca gaaactgcgg
    15721 caggatttcc ttaacagcct gacggtggag atgttaaatc aggatctgcg tcagcagttg
    15781 tcgaatgata tggcgttaat actgctgcag ccgaaaggcg agccggaatt taacatgaaa
    15841 gcgttgcagg cggcctggga tcaaatcatg gccccatcga ctgctgctgc cgccacctct
    15901 gtcgccacgg atgacgtaca tcctgaagtg acggatattc cacctgcaca gtaatgcctg
    15961 atggtgatga gttagcacaa ccatcaggta tttatggatg agctggatag aggggtaaca
    16021 ctttatccag ccttttgcat atgctgcgtt tacgctggca tcgcctcacg cgggataatc
    16081 gcgccgcgat actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc cgcgttttcc
    16141 gcactgccgc ccgtcaaaca taccgccaga taaccggcgc tgaaagagtc gccagctgcg
    16201 gtggtatcga tcactttttc tttcggcagt ttcaccgccg gaacatccac taacccttcg
    16261 ccagcaatgg acaccaggca agaatccgcc ccgcgtttca ccaccacttc tttcacgccc
    16321 gcgttatggg tgcgcgcaat gacgtcttcc accggctgtt gaccccacag cgcgtcttca
    16381 tcgtccagcg tcaggaaggc gatatccgtg cattccagca tttgttggta cacctgctgt
    16441 gtctcttctt tgctggccca caggcgcgga cgatagttat tgtcgaaaat cacttttccg
    16501 ccgttggcgc ggcattcgcg cagcagggaa agcagctttt cgcggctggt cgggcttaag
    16561 atcgccaggc taatcccgct caggtagaga taatcgaaat tcgccagctc ttcgcaaatc
    16621 gccgcagact gctcactctc cagccagaat ttggcggcgg cttcgttccg ccagtagtag
    16681 aacgtacgct cgccggtgct gtcggtttca atgtagtaaa ggcccggcag acggttttcc
    16741 atccgttggg tcagggaagt atcaacgttc tcgccgtgcc aggcgtccag catttgctgg
    16801 ctaaaactgt ccgttcccag cgccgttacg taatgaacgg ttaatgccgc aggatcgacc
    16861 tgacgggcga tatagacgga agtgttcagg gtatcgccgc cgaaaccgcg cttaacgtcc
    16921 gcgcctttct cggaaagctc aatcatgcat tcgccaatca cggcaatctt tttggacata
    16981 gtcgtgaacc tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt ttgctggtca
    17041 accatattaa aacagtgttc cattattttt ttgagccaga acgtattcct gaaagattcc
    17101 gttgtggagt gaggaaattt accccggcca ggcggtaaag ttctgccctt gcgcgccgat
    17161 aatctttgtc gagtccgggc agcatcactt ttaaacacag gacatctttg atgataaggc
    17221 aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag gaacggcgtt
    17281 tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca tgcggacggt
    17341 taatggccgt ggagctatta acggtggtca cgcatccctt gaacccttcg caacgcctgc
    17401 cgccggatcg ctattttact gaaatcaccg tcagccatcg gatggaggtt gtgaaagagc
    17461 agattgattt gctggcgcaa aaagccgact tctttgtaga gcacgacctg ctggcatcgg
    17521 tcaatattga tggccctacg ctcatcgccc tgcgtcagca accaaaaatc ctgcgccaaa
    17581 ttgagcgtct tccctggctg cgtttcgaac tggtggaaca catccgcctg ccgaaagatt
    17641 caacctttgc ctcgatgtgt gaatttggcc cgctgtggct ggatgatttt ggcaccggga
    17701 tggcaaattt ctctgcgcta agtgaagtgc gttatgacta catcaaaatc gcgcgagagc
    17761 tgttcgtgat gctgcgtcag tcgccggaag gccgcacact cttttctcag cttttacatc
    17821 taatgaatcg ctattgtcgc ggggtgattg tcgagggcgt agaaacaccg gaagagtggc
    17881 gtgatgttca gaactcgccc gcattcgccg cacaaggctg gtttctttca cgcccggcac
    17941 cgatggaaac gctgaatacg gcggttctgg cgctataagc tgcctcattt tccgctgggc
    18001 tggactatct ttaggactgg cacaggaaga atatgaggaa gcgaacgatg agcaaggcag
    18061 gcaaaataac cgctgcgatt tcaggggctt tcttgttgtt gattgtcgtg gcgatcattt
    18121 tgattgcaac ttttgactgg aatcgactca aaccgaccat caaccagaaa gtctctgcgg
    18181 agttgaatcg tccgttcgct atccgtggcg atctgggcgt ggtgtgggag cggcaaaaac
    18241 aagaaactgg ctggcgcagc tgggtgccgt ggcctcatgt acatgcagaa gacatcattc
    18301 ttggtaatcc accggatatt cccgaagtca cgatggtgca tttgccgcga gtagaggcaa
    18361 cgctggcacc gctggcgctg ctgaccaaaa cggtctggct gccgtggttc aagctcgaaa
    18421 agcccgacgc gcgcctgatt cgcctctccg aaaagaacaa taactggacg tttaatcttg
    18481 ccaacgatga caacaaagac gcgaatgcaa agccgtcggc atggtcgttt cggctggata
    18541 atattctttt cgatcaaggg cggatcgcca ttgatgacaa agtaagcaaa gcggatctgg
    18601 aaatttttgt tgatccgtta ggcaagccgc tgccgttcag cgaagttact ggatcgaaag
    18661 gtaaagcgga taaagaaaag gtgggcgatt acgtttttgg cctgaaggcg cagggacgtt
    18721 ataacggtga accgctcacg ggtacgggaa aaataggcgg tatgctggcg ctgcgtggcg
    18781 aagggacgcc gtttccggta caggctgatt tccgttcagg taatacccgt gttgcttttg
    18841 atggcgtcgt gaatgaccca atgaagatgg gcggtgtcga tttacggctt aaattttctg
    18901 gcgattcact gggtgatctc tatgaactga cgggcgttct gctgcccgat accccgccgt
    18961 ttgaaacgga tggtcggctg gtagcgaaaa tcgacactga aaaatcgtcg gtctttgatt
    19021 atcgcggttt taatgggcga attggcgata gcgatatcca cggttctctg gtctacacca
    19081 ccggaaagcc acgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg cggctggcgg
    19141 acctgggacc gttgattggc gttgattccg ggaaaggggc agaaaagtcg aaacggtctg
    19201 aacagaagaa gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct tatgaccgct
    19261 tcgaaaccga taaatgggac gttatggatg ccgatgttcg cttcaaaggg cggcgcattg
    19321 agcatggcag tagcctgccg attagcgatc tttctactca tatcatcctc aaaaatgctg
    19381 acctgcgcct gcaaccgctg aaatttggca tggcgggcgg cagcattgcg acgaatattc
    19441 atctggaagg cgataaaaag ccgatgcagg ggcgggcaga tattcaggct cgtcgactga
    19501 aactgaaaga actgatgccc gatgtggaac tgatgcagaa gacgctgggg gaaatgaacg
    19561 gtgacgcgga actacgcggt agcggtaact cggtggcggc gcttttaggc aacagtaacg
    19621 gcaacctgaa actgttgatg aatgacgggc tggtgagccg caacctgatg gagattgttg
    19681 ggctgaatgt cggcaactac attgtcggtg cgatatttgg tgacgatgag gtgcgggtga
    19741 actgcgcggc ggcgaatctg aatattgcca acggcgtggc gcgcccgcag atttttgctt
    19801 tcgatactga gaacgcgttg attaatgtta ccggcacggc aagttttgct tcagaacagc
    19861 tggatttgac tattgatccg gagagtaaag ggattcggat tatcacactg cgttcgccgc
    19921 tgtatgtgcg tgggacgttt aaaaatccgc aggctggggt gaaagccgga ccgctgattg
    19981 cccgtggtgc tgttgctgcg gcactggcaa cgctggtaac accgacggcg gcgttactgg
    20041 cactgatctc accttccgaa ggggaggcta atcagtgtcg gacgatattg tcgcagatga
    20101 agaagtgatg ataattgccg gatgtctgac atccggcaat taaacagatt tagatttttg
    20161 gcaaagtgtg ctttgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaatcc
    20221 ttgtgaattc aatatattgt atggaaattc agtcctgata agcgtagcgc atcaggcttt
    20281 tcacacttat tacaacgact gatgtcgcgt ctcatgggtc agcagcaggg cgattaacgt
    20341 caagccagcc atcgccgcca gatataaccc caccgcacct aacccgtagt tagtctgcaa
    20401 ccaggctgcg atatacggtg caacggaagc cccgagaatc gacgctacgt tgtaagagaa
    20461 tgatgctccg gtgtaacgca cttctgttgg gaacagctct ggtaacagcg cgcccatcgg
    20521 cccgaaggtc agccccatca gacttaaccc cagcagcagg aaggcaaaaa ccagaatcgg
    20581 gttgccagaa ccgagcagcg ggttaaaggc gaacagcgcg aacaggatga tcagcgtggt
    20641 gataattacc atgcttttac ggcgaccaaa ggcatcagcc agtaatccgg cgactggcac
    20701 catcacgcca aaaccaataa ctgccatcat cagcatccac aacacttcgt tacgcggcag
    20761 gccaagccca actggcgcgg cggcggtact gaaggtcata gagtagaccg tcatgatgta
    20821 aaacagcgta taggttgcca gcatgatgaa cgtacccagt acggttacgc gaacatgttt
    20881 ggtcagcagc gtacccagcg ggatcttcac ctgttttttc gctttagcga ctttctcaaa
    20941 caccggcgac tcatgcagcg acacgcgaac atacaggccg ataatgacca gtaccgccga
    21001 gaagataaat ggcacacgcc agccccagct cataaactgc tcgtcggtca gtagccagga
    21061 aagcagcaag aaagtgccgt tggcaaagaa gaagccaatc ggtgcgccca gctgtgggaa
    21121 ggagccatac agtgcacgtt tgcgcggtgg ggcgttttca gtcgccagca gcgccgcgcc
    21181 gccccattca ccgcctaagc ccagaccctg accaaatcga gccagcgcca gcagcagcgg
    21241 ggcgaaaata ccaatcgtgg catagcccgg cagcagacca atcaccacgg ttgaaatccc
    21301 catcgttagc aacgaggcga ccagcgtcgc tttacgccca acgcgatcgc caaaatgacc
    21361 aaaaacggca gagccaatgg ggcgcgcgac gaaggcgatg gcgaaggtgg cgagcgactg
    21421 tagcgttgct gccgttggat cgccctgcgg gaagaagata tgcggaaaca caataacggc
    21481 cgcagtggcg taaatgtaaa agtcgaagaa ctcaatggcc gtgccaatga gagaggcgac
    21541 aaggacttta ttacgcgagt tgatcggcgt gtattcttgc tcgtggtcga gtgttgtggc
    21601 tgttgcttgc ataatctttt cttatttttt ggctaacgaa tagccatatt acgcacagca
    21661 aaagtgatat ttcaatgtgt aacaaatcgc ggaaatggtc aaaaaccagg caaaaagggg
    21721 ataatgttta ggccagtgat ttttcttcta tgaaatgctt cacataattt tataaatagt
    21781 ggataaaact ggtttcaggt taaataaaag ttaccaactg gatttatatg ctggggttat
    21841 gagttggaat gaaattgttt gctcccaaat agatggctga agaggctctg gggaagagtt
    21901 tcaagaggtt atgtgcgcat tatagccgga tgcgatgctg gcgcatctta tccggcctac
    21961 ggggagccag gccgaataaa atcgtatgcc ggataaggcg tttacgccgc atccggccta
    22021 ttttaaggct gcgttttccc tggcattcgc gggtcgtctt tatattcggc ggtggcaatc
    22081 cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga aaaatgccat cagccccagc
    22141 acggaaccaa atgctgcgcc agagggggat ttcatcagcg atggcagggt gtaggtcatc
    22201 acgattttaa tcacttcaaa accaatagca gccagaaatg ttccgcgaat cagggctttt
    22261 ttgcgcggac ggtggcgcgg taaccgccag aagatccaga agaaaagcag atagttggcg
    22321 aagatggaaa tcgccaggcc aatcaaccgc cacgtcggtt tcagccactc aatgctgttc
    22381 aggtgcaggg cgctaataat catttgctgc gccgaaccgg caaccgaggt gatcgaaagc
    22441 gtcacaatca gcgcaatcaa caaaccaatc agcgaaataa aatcacgtag atattttacc
    22501 cagaacttct cctgatcttg cggcgagcgt tcccagacat cgcgcgactg ggcgcgaatc
    22561 gcttcacgca ggttacccat ccagttgatg ccggaataaa gcgccacagc caggccgaca
    22621 agccctacag tcgtacgctg ctgaacggcg gtgttgatgg tgtttttcaa cgtggcggct
    22681 agcgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga tatcctgtag caacatcgga
    22741 tgggaggcca gcacaaagcc ccctgcggca aacgacacca tcaaaatcgg gatcatcgac
    22801 aaaaatgaga aataggtgat agccgcacca aactggttac ccagccgatc gttaaagcgt
    22861 tctgtcgcgc gaatcaggtg cgcaataacc ggttggcgtt ggactttttc ggcagtggtg
    22921 gtgacggttt ccagcgcctg gctaactttg ccgcctttct cgctattatc caactgctta
    22981 atcggctcgt gctccagatc ctgggtggga cgtttgatct cgttttcctg cgtcatccgc
    23041 tcttttgtcc ttctgttgtg gtcattctta aaaagtatag tcggtcagtc caggtactct
    23101 ttcattacgc cagccagcca ctgcataaac aggtttacac gccgggaaag ctcccgacgc
    23161 tgcggataaa ccagcgaaag ggagagcggc tcggcacggt agcctggcaa cacttcaata
    23221 agctgcccgg cacgcagggc ttcgcgcacg gcaatgcgcg ggatctgaat aatccccaga
    23281 ccggcaagac cggcggcgag atagttttcg ctactgttta ccgtcaacat gccgccagac
    23341 ttaaaccact ggacgccatt aacgctggca acctcaaaac ctaacggatg tacacccagg
    23401 tgcggtgtat aacgcaccat tgcgtgtgaa gtcagatcat cggggctttg cggataacca
    23461 aagcgcgtca gatagtgcgg actggcacag ttgaccatgg tcagtttgcc gaggggacgg
    23521 gcgataacgc catcttccgg taacgcgcca gtgcgtatca cgcaatcaaa accatcatga
    23581 agaatatcta ccggacggtt atggctactc agttccagct caattcccgg atgctgatag
    23641 agaaattccg acagacgcgg cagtaacagg tttttcgcga ttcctgacgg gatgtcgatg
    23701 cgtaatttac cgctgatact ggtggcatcc tgttgaaaca gaccgtccag ttcgctgaga
    23761 ttactcaaca catcttttgc ccgttgataa taggtcatgc cttctggcgt gagtttgacc
    23821 cgtcgcgtag tgcgctggag aagctgggtg ccaagttgat gttccagtgc ctgtatctgg
    23881 cgcgaaacac ttccctttgg caaagcaaag aaatccgccg cgcgggaaaa actttccagc
    23941 tccgcgactt tgatgaacaa ctgcattgcg tgaattttat ccatttcttt gaccgattgt
    24001 tgttcacagc gaaacagtaa cgcgcatatc gcattgttta ttgttttttc taacatctaa
    24061 tgccgggttt cacaatgttg tcatgacgaa tgtggcgatt tctcagcagg aaaagggcgg
    24121 aatagcttta tgagaaaggt gaatcccctt aaacagagcg tatttttcag tgattccctt
    24181 taaagtgaat agaggacgta ttggctattt gtgcttattt ttgtctcatt gccatttgtt
    24241 attttggctt gtagtcgcgg aatttcattt ttaattgcat gcgtactaac caccaatata
    24301 aggaaatcct caaaattgtt ttaaatagtg ccctcgacat gtaatttcag gcgtttagct
    24361 gtaactattt atatcggtaa ctattttcta cgcgtcatct ctgtagaatg gccaccgcca
    24421 aatggcgaat tcttattttt tctcgcgggt aatacccttc cactacttaa gtctttttaa
    24481 cgtattcagg tggagattca gttatgcaaa tagtcatgtt tgacaggcag tcaatattta
    24541 ttcatggaat gaaaatcagt ttacagcagc gtattccagg agtgagtatt cagggggcca
    24601 gtcaggcaga cgagttatgg caaaagctgg aaagttaccc tgaagcctta gttatgctcg
    24661 atggcgatca ggatggtgag ttttgctact ggttgctgca aaaaaccgtg gtgcagtttc
    24721 ctgaggttaa ggtgttaatc acggcgacgg attgcaataa acggtggtta caggaagtta
    24781 ttcattttaa tgtgctggcc attgtgcctc gtgattcaac cgtcgagaca tttgcgttgg
    24841 cggtaaatag tgcggcgatg gggatgatgt ttctgcctgg agactggcgg acgacgccgg
    24901 aaaaggacat taaagatctc aaatcgttaa gcgcccgcca gcgggagatt ttaaccatgt
    24961 tagcggcggg cgaatcaaat aaggagatcg gcagagcgct gaatatcagt accggaacgg
    25021 taaaagcgca tctggaatcc ctgtatcgcc gtctggaagt gaaaaatcgc acccaggcgg
    25081 caatgatgtt aaatatctcc tcctg
//
LOCUS       DAJTYZ010000054        24789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000054 DAJTYZ010000000
VERSION     DAJTYZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.054"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1395)
                     /gene="pdeC"
                     /locus_tag="OO596_002092"
     CDS             complement(<1..1395)
                     /gene="pdeC"
                     /locus_tag="OO596_002092"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR6717950.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHI"
     gene            1825..2105
                     /locus_tag="OO596_002093"
                     /pseudo
     CDS             1825..2105
                     /locus_tag="OO596_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2204..2740)
                     /gene="ssb1"
                     /locus_tag="OO596_002094"
     CDS             complement(2204..2740)
                     /gene="ssb1"
                     /locus_tag="OO596_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6717951.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            2994..5816
                     /gene="uvrA"
                     /locus_tag="OO596_002095"
     CDS             2994..5816
                     /gene="uvrA"
                     /locus_tag="OO596_002095"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6717952.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(5851..6207)
                     /locus_tag="OO596_002096"
     CDS             complement(5851..6207)
                     /locus_tag="OO596_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6717953.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6211..6627)
                     /locus_tag="OO596_002097"
     CDS             complement(6211..6627)
                     /locus_tag="OO596_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6717954.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(6738..7451)
                     /gene="aphA"
                     /locus_tag="OO596_002098"
     CDS             complement(6738..7451)
                     /gene="aphA"
                     /locus_tag="OO596_002098"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6717955.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            7853..8075
                     /locus_tag="OO596_002099"
                     /pseudo
     CDS             7853..8075
                     /locus_tag="OO596_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8579..9772)
                     /gene="tyrB"
                     /locus_tag="OO596_002100"
     CDS             complement(8579..9772)
                     /gene="tyrB"
                     /locus_tag="OO596_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6717956.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10025..11104)
                     /gene="alr"
                     /locus_tag="OO596_002101"
     CDS             complement(10025..11104)
                     /gene="alr"
                     /locus_tag="OO596_002101"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6717957.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11157..12572)
                     /gene="dnaB"
                     /locus_tag="OO596_002102"
     CDS             complement(11157..12572)
                     /gene="dnaB"
                     /locus_tag="OO596_002102"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6717958.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            12655..13638
                     /locus_tag="OO596_002103"
     CDS             12655..13638
                     /locus_tag="OO596_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6717959.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(13804..14046)
                     /gene="pspG"
                     /locus_tag="OO596_002104"
     CDS             complement(13804..14046)
                     /gene="pspG"
                     /locus_tag="OO596_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6717960.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14180..15217)
                     /gene="dusA"
                     /locus_tag="OO596_002105"
     CDS             complement(14180..15217)
                     /gene="dusA"
                     /locus_tag="OO596_002105"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6717961.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(15580..16565)
                     /locus_tag="OO596_002106"
                     /pseudo
     CDS             complement(15580..16565)
                     /locus_tag="OO596_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            16883..17398
                     /gene="zur"
                     /locus_tag="OO596_002107"
     CDS             16883..17398
                     /gene="zur"
                     /locus_tag="OO596_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6717962.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(17440..17649)
                     /locus_tag="OO596_002108"
     CDS             complement(17440..17649)
                     /locus_tag="OO596_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6717963.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(17765..19090)
                     /gene="dinF"
                     /locus_tag="OO596_002109"
     CDS             complement(17765..19090)
                     /gene="dinF"
                     /locus_tag="OO596_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6717964.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19163..19771)
                     /gene="lexA"
                     /locus_tag="OO596_002110"
     CDS             complement(19163..19771)
                     /gene="lexA"
                     /locus_tag="OO596_002110"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6717965.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(19881..20249)
                     /gene="dgkA"
                     /locus_tag="OO596_002111"
     CDS             complement(19881..20249)
                     /gene="dgkA"
                     /locus_tag="OO596_002111"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6717966.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            20420..22843
                     /gene="plsB"
                     /locus_tag="OO596_002112"
     CDS             20420..22843
                     /gene="plsB"
                     /locus_tag="OO596_002112"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6717967.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(22998..23870)
                     /gene="ubiA"
                     /locus_tag="OO596_002113"
     CDS             complement(22998..23870)
                     /gene="ubiA"
                     /locus_tag="OO596_002113"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6717968.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(23882..24379)
                     /gene="ubiC"
                     /locus_tag="OO596_002114"
     CDS             complement(23882..24379)
                     /gene="ubiC"
                     /locus_tag="OO596_002114"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6717969.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(24602..>24789)
                     /locus_tag="OO596_002115"
     CDS             complement(24602..>24789)
                     /locus_tag="OO596_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717970.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
BASE COUNT         6429 a         6362 c         5947 g         6051 t
ORIGIN      
        1 gatgtgttcc gcaatcaaat gactggtttt gtgggtggtc agcgtttcaa caaacgattt
       61 atcgattttc aaaatatcga cattcaatga tttaaggtta tgcaagttag agtagccaat
      121 accaaaatca tcaattgcca cttcgtaacc agcctggcgg aaagccagaa taatcggcgt
      181 cattttttcg acatcaagaa atgcatgctc agtcacttca aatttaattt gctgcggacg
      241 caccgcgtat tgctctgttt tctgattgat tcgcgctatc aaccgtgacg tatgaaaatc
      301 ggaggccgac aggttaatag aaacatagcg atcggcatgt gttgccaagt acgcgcccag
      361 atcgcggaag acattatcaa taacataatc agttatctgt tctatcatcc cctccttttc
      421 tgccagcgga ataaactctg ccggattcat tatttgcccc tgctcaccaa gccaacgtaa
      481 caacgcttca gcgccgatac atttttctgt tttgatatcg attattggct ggtaataaag
      541 acaaagttga tgtttttcga gggcgcgttg caatttacgt ttgggagata aatagttttg
      601 tcgaatacgt agccagagta atagcagaac aagactcccc aaaataccgg cgggcaacgc
      661 aaaaataaga tgattataaa aatgggttat aagacgttga tatgaagtcg caacaatggc
      721 tgcaattggg cgttttgtcg aataaactgt cgcatataaa tagccatttc tttgtacggt
      781 taaatccttc aaatgaatca gcggagaaaa cgttgctgcc gaggcctctt tgcttaacga
      841 gaaaaaggtt ttcgtcaccg tatcatacac accccattgc aatgtcgggt catcagacat
      901 cacttcactc cagaagagag ggttgataac cgccacataa tttccccgct gcatataggt
      961 cattttatag ccagagaaaa aaggcgtatc gcggtaataa tagatagaaa cgttaggttc
     1021 acgcttataa tcggccggtg caatcgtata gccgtttaca ggcgctatca gcgatgagca
     1081 taaaaaatgg ttatcacggg catagatcaa ttcattaata taaagatagc cacgaataat
     1141 attcaacatt cgcttttgat gggctggagt gcaaacttgc ccctgatagc gttcagctgc
     1201 atcgctcact aaatctgcct ggcgaatgac cagctcggat ttatccagtg ccagttgagc
     1261 aaaggttcgg agctgattat tcacttctga ttttgcccaa aggaaggcaa tccatagcga
     1321 aagaatgatg ggaaagagaa ctaaaaagat aatgcccggc aacgccagta attggtgtcg
     1381 tgcacgatga ctcattgcta tcaatttcct gcctgtttat acccggccat tgcaaggtga
     1441 aaaccctggg taccgacgtc tttagataaa tcttacggta taaatagcac ttttgcctga
     1501 tttgaaattg atacctttct aatcgtcacc tggatgaaaa taattcattg atattcaaaa
     1561 ttaatttcct acaggaatgc agaggcggcg ggaagatatg cccttaatgc cactgaaaaa
     1621 ccacataatc tgtacagcaa taaaagtcac ggcctaatat ggctaacaaa caatcaattg
     1681 tgatatagct cacaaaatta atgaaacaaa cagagtgttt catttttgtg ttctgcaagc
     1741 cccgcgatag ccagagtgta ccacgcctcc cgtgaacaac gccgcgctgt ccagggcatc
     1801 ggctcttttc agaggaattg tttgatggct accctcacca ctggcgtggt tcttcttcgc
     1861 tggcagcttc ttagtgccgt aatgatgttt ctggccagca catcaacatc cgttttcgtc
     1921 ggtctgatta tgtcgggctt gcggtgatca gcagcggtct gggcgttgtt tccgcctgtt
     1981 ggttcgcaat ggggttgctt ggcatcacaa tggcggacat caccgctatc tggcacaaca
     2041 tcgagtcggt gatgatagaa gagatgaatc agacaccgcc acaatggcca atgattttga
     2101 cctgatacgt agaagcctcc ataacggagg cttctttttt atggctggca gatgctttaa
     2161 tcatccacct taaaacaata taacctattg ttttaatgac aaatcagaac ggaatgtcat
     2221 catcaaagtc catcggcggc tcgttagacg gcgctgccgg agcggactgc tgcgggcgag
     2281 actgcgcgcc gccgctgaac tgattgccac cctgcggctg ctgaggctgc ccccaaccgc
     2341 cctgcggctg accaccaccg atattaccac ctgccggagc gccaccaccc tgacgaccac
     2401 ccagcatctg catggtgccg ccaacgttca ccacgacttc tgtggtgtag cgatcctgac
     2461 cggattgatc ggtccattta cgggtacgca gctgaccttc gatataaacc tgagaacctt
     2521 tacgcagata ttcgctggcc acttctgcca gtttgccgaa cagcacaacg cggtgccatt
     2581 cagtctgttc tttcatctcg ccggtcgctt tatcacgcca ggattcggaa gtagccagcg
     2641 taatgttggc aactgcgcca ccatttggca tgtagcgtac ttccgggtcc tgacccagat
     2701 taccaacgag aataaccttg tttacgcctc tgctggccat gttcgtgtct cctgaaaaaa
     2761 atcgttctga ataagtgtaa acgcgcgatt gtaccattac caatagcgct tttactatgt
     2821 tgtgacctcg gttccgggaa acaaacctgg ccagacattg ttacacaaca ctccggataa
     2881 tgcattccaa tactgtatat tcattcaggt caatttgtgt cataattaac cgtttgtgat
     2941 cgccggtagc accatgccac cgggcaaaaa agcgtttaat ccgggaaagg tgaatggata
     3001 agatcgaagt tcggggcgcc cgcacccata atctcaaaaa catcaacctc gttatccccc
     3061 gcgacaagct cattgtcgtg accgggcttt cgggttctgg caaatcctcg ctcgctttcg
     3121 acaccttata tgccgaaggg cagcgccgtt acgttgaatc cctttccgcc tacgcgcggc
     3181 agtttctgtc actgatggaa aagccggacg tcgatcatat tgaggggctt tctcctgcca
     3241 tctcaattga gcagaaatcg acgtctcata acccgcgttc tacggtgggg acaatcaccg
     3301 aaatccacga ctatttgcgt ttgttgttcg cccgcgtcgg cgaaccgcgc tgtccggacc
     3361 acgacgtccc gctggcggcg caaaccgtca gccagatggt ggataacgtg ctgtcgcagc
     3421 cggaaggcaa gcgtctgatg ctgctcgcgc caatcattaa agagcgcaaa ggcgaacaca
     3481 ccaaaacgct ggagaacctg gcaagccagg gttacatccg tgctcgtatt gatggcgaag
     3541 tctgcgatct ttccgatccg ccgaaactgg aactgcaaaa gaaacacact attgaagtgg
     3601 tggttgatcg cttcaaggtg cgtgacgatc ttacccaacg tcttgcggag tcgtttgaaa
     3661 ccgcgctgga gctttccggt ggtacagcgg tagtggcgga tatggacgac ccgaaagcgg
     3721 aagagctgct gttctccgcc aacttcgcct gcccaatttg cggctacagt atgcgtgaac
     3781 tggagccgcg actgttttcg tttaacaacc cggcaggtgc ctgcccgacc tgtgacggcc
     3841 ttggcgtaca gcaatatttc gatcctgacc gcgtgatcca aaaccccgag ctgtcactgg
     3901 ctggcggtgc gatccgtggc tgggatcgcc gcaacttcta ttatttccag atgctgaaat
     3961 cgctggcaga tcactataag ttcgacgtcg aagcgccgtg gggcagcctg agcgcgaacg
     4021 tgcataaagt ggtgttgtac ggttctggca aagaaaacat tgaattcaaa tacatgaacg
     4081 atcgtggcga tacctccatt cgtcgtcatc cgttcgaagg cgtgctgcac aatatggagc
     4141 gccgttataa agagacggaa tccagtgcgg tacgtgaaga attagccaag tttatcagca
     4201 atcgcccatg cgccagctgc gaaggaacgc gtctgcgtcg ggaagcgcgc cacgtgtatg
     4261 tcgagaatac gccgctgcct gctatctccg acatgagcat cggtcatgcg atggaattct
     4321 tcaacaatct caaactcgca ggtcagcggg cgaagattgc agaaaaaatc cttaaagaga
     4381 tcggcgatcg tctgaaattc ctcgttaacg tcggcctgaa ttacctgaca ctttcccgct
     4441 cggcagaaac actttccggc ggtgaagccc agcgtatccg tctggcgagc cagattggtg
     4501 cgggcctggt tggcgttatg tatgtactgg acgagccgtc tatcggcctg caccagcgcg
     4561 ataacgagcg cctgttgggt acgcttatcc atctgcgcga tctcggtaat accgtgattg
     4621 tggtggagca tgacgaagac gcaattcgcg ccgctgacca tgtgatcgat atcggtcctg
     4681 gtgcgggtgt tcacggcggt gaagtggtcg cagaaggtcc gctggaagcg attatggcgg
     4741 tgcctgaatc gttgaccggg cagtacatga gcggtaaacg caagattgaa gtgccgaaga
     4801 aacgcgttcc ggcgaatcca gaaaaagtgc tgaagctgac aggtgcacgc ggcaacaacc
     4861 tgaaggacgt gacgctcacg ctgccagtcg gtctgtttac ctgcatcaca ggggtttcag
     4921 gttccggtaa atcgacgctg attaacgaca cactgttccc gattgcccaa cgccagttga
     4981 atggtgcgac catcgccgaa ccggcaccgt atcgcgatat tcaggggctg gagcatttcg
     5041 ataaagtgat cgatatcgac caaagcccaa ttggtcgtac tccacgttct aacccggcga
     5101 cctataccgg cgtgtttaca cctgtgcgcg aactgtttgc gggcgtaccg gaatcccgtg
     5161 cgcgtggtta tacgccagga cgtttcagct ttaacgtccg tggcgggcgc tgcgaagcct
     5221 gtcagggcga cggtgtgatc aaagtggaga tgcacttcct gccggatatc tacgtgccgt
     5281 gcgatcagtg caaaggtaaa cgctataacc gtgaaacgct ggaaattaag tacaaaggca
     5341 aaaccatcca cgaagtgctg gatatgacca tcgaagaggc gcgtgagttc tttgatgcgg
     5401 tgccagctct ggcgcgtaag ctgcaaacgt tgatggacgt tggtctgacg tacattcgcc
     5461 tggggcagtc cgcaaccaca ctttctggtg gtgaagccca gcgcgtgaag ctggcgcgtg
     5521 agctgtcaaa acgcggcacc gggcagacgc tgtatattct cgacgagccg accaccggtc
     5581 tgcacttcgc cgatattcag caactcctcg acgtgctgca taaactgcgc gatcagggca
     5641 ataccattgt ggtgattgag cacaatctcg acgtgatcaa aaccgctgac tggattgtcg
     5701 acctgggacc agaaggcggc agtggcggcg gcgaaatcct cgtctccggt acgccagaaa
     5761 ccgtcgcgga gtgcgaagct tcgcatacgg cacgcttcct caagccgatg ctgtaatcgt
     5821 taaggccgct ttctgagcgg ccttttcctt tcagagttgt accagcaatt tacgcttttc
     5881 ttccggtagt aaattcactg cctgctgata agacgcatcc accagataat agatttgcga
     5941 atccggcagc gaaccgtcga gatacacggt gctccagtgc gctttattca gatggcggct
     6001 aggacgcaca tcgctgtgct gctgacgtag cagctccgcc agctccgggc tggttttcag
     6061 cgaaacagct gggcgatttt ctacttcttt caccatcgca aacagtacat cttccacttt
     6121 gatctgcgtc gctttccagt cgttatgcac gctctgttct gcgcctggtt ttgccatgca
     6181 atattgtagc aactccgaaa tggtcatttt ttactcccct tgtagtgtcg cgatgatgcg
     6241 acgcgatccg ccgtggatgc gatgttcccc cagccagatg ccttgccagg tgccggtctg
     6301 aatacgccct ttatgtaacg gcaatacaag cgatgttccc agcattgagg atttgatatg
     6361 agaaggcata tcgtctgctc cctcatagtc atgctcataa tttccgttgt cgggaacggt
     6421 gcggaggaaa aaacgctcca tgtcgtggcg tacggtggga tcgcagttct cattaagtgt
     6481 cagagaggcg gaggtatgtt gcagcaacag atgcagtaag ccgatgttaa cgcgcggcat
     6541 atcagccagc tgattcagaa tttcatccgt taccagatga aacccacgag atttggcgct
     6601 aagcgtgagc gtcttttgat accacatgtg ctgctccttg ataaaactct cttaatcagt
     6661 ttgcagcaag acagcgaaag gataaaggtg tgattaaaaa aacagcattc gggagagcat
     6721 cacgctctcc cgctctgtca gtattctgaa ttgacgatca cctcttcacc aaacgcaccc
     6781 gcttgtggca agggtttgta ggtagagttg gaggcgcgca gaatgcggat accacgagcg
     6841 ccgacatcgc gtgcggcggt aatatcgtta tcggaatcgc cataaaaaat tcggatattt
     6901 ttatcctgca gccattgcga ttttgtattt tgccctggtt tatcgcccgc aaagattacc
     6961 ggattcatgt tggtggcagg aatatgaaaa ttatccgcca gcgtttttga aaccgtttct
     7021 gttttcgtcg ggctacgacc agtcacaaag aagatcgcgt caccgcggcg tacatgcata
     7081 tcaatcagct ggcgagcgac ctcttttgga atgctgaatt catcccagcc attattcatt
     7141 ttttcccaga acacaggatt tttcagataa tcttcgcttt ctggcgagaa ggtttttttg
     7201 ccgcgccaga accccggact ggaaaaaagt accgtgtcat cgatatcaaa ccccaccgcc
     7261 attggcggac gccctgcgag gctattttca atttgtgcga ccgaaaccca atgaattggt
     7321 gcctgttcag caagcctggc aacgttagtc ccagggttaa gcggtgaagg agatgaggcc
     7381 agggcaacag cggaactgtt tagagcgaac aataagcaaa cggcactgat tgcctgtgtg
     7441 atcttgcgca tatttttccc taaatagtca gtatgttgaa actttttatt gtgagatttg
     7501 ttgcaaaaac catctgacca taacgccagc tgccggggaa aggaaggatt atctgcggtt
     7561 tttgtgatga aaagcaggat gattatattt aacccagtaa atacgttcct gtcttttttt
     7621 gtataaaaaa tgtttattac tcgatgtaag gatacattaa ttttacgttt attatgatga
     7681 atcctctgca aatgagcgaa atttgaaaga acatttatct gctgtacgat ttaatgaact
     7741 taatgataca gcattacaaa aacaatcgaa gtttataaag atgattcctg attgaccaga
     7801 accctatcct acaatggtga ctaattttat tactcccgat ggagatgatg atatgaatat
     7861 atcatatgtg aatagcaaca aaacgacatc tttacctgtt gagctagatg cactaaataa
     7921 taaagacatc tcttatgcca aagatttttc ttatgctaaa gatttctttt tatacataga
     7981 aacacagtta aaaattgcaa aagatttttg tagacctgga gaagaagtat caagttctat
     8041 tgcaagtaaa gtttttcacg catttatcga tttagttaac aaaatcaggg gtaagaaaga
     8101 ctttatgtat atttgcacgc tttgctgttt tgccgaggag gttaaaggtg attattctca
     8161 ttacaggacc tttttatttg atattggtaa tcaatataag gttaaactta cacaaagcgg
     8221 aaaaaaagag ttctctttaa cattagaatt taacgatact ataattgaat ctcagaaagt
     8281 cacaggcaat aaggcaaagt atattcttga ggatatagaa atattctatc gtaacaaacc
     8341 cgatacttat tattaaattt aataatggtt agatgtgcag aaattattac tccacacgaa
     8401 aaaataggga aaggaattcc agataattgg gataaactta acaataaaag aactgacaac
     8461 gttttattat ctctcacgta gaacaacggc atcaaaaaaa tcatctacaa agaaatagtg
     8521 aaagcgacga cagtttaatt tcactgcagg ctgggtaact ccagcctgct ttcctgcatt
     8581 acatcaccgc agcaaacgcc tttgccacac gttgtacatt tgccgtattt aacccggcga
     8641 cacacatgcg accgctggcg atgagataga caccaaattc ttcacgtagt cggtcaacct
     8701 gagcggcact taaaccggta taactgaaca tgccgcgctg attaagcaga taatcgaaat
     8761 tgcgttctgg catctctgtg cttaatacct tcaccaattc ctgacgcatt gccagaatgc
     8821 gagtacgcat ctcttctact tccgccagcc agctggcttt caatgcctcg tcattcagca
     8881 ctgcagccac cacctgcgca ccaaaattcg gcgggctgga gtagttgcgg cgaactgttg
     8941 ctttcaattg ccccagtacg cggccagcgg cttcggcatc ttcacacaga acagaaagtc
     9001 cgccgacgcg ctcgccgtaa agggagaaaa ttttcgagaa cgaattgctc accagagcgg
     9061 gtaatccagc gctggcaatg gcgcgaatag cgtaggcatc ctcttccata ccggcactaa
     9121 atccttgata ggcaatatcg aggaatggga taagctcgcg ggctttgaga atttcaatca
     9181 ccgcatccca ctgatcatta gtgagatcgg cacccgttgg gttgtggcaa catggatgca
     9241 gcaacacaat actgcgggca ggtaatgttt tcagcgtcgc caacaggtca ttaaagcgca
     9301 cgctgttagt cgcttcgtca taccaggggt aagtactcac ttcgaatcca gccccggcga
     9361 atattgctac gtggttttcc caggtaggat cgctgaccca gacgcctgat tccgggaagt
     9421 agcgtttcag gaaatccgcg cccactttca atgcccctga gccgccaagg gtttgaatgg
     9481 ttgctacgcg ctgttgttgc agtaccggat ggtccgcacc aaacagcagc ggcgcaatgg
     9541 catggcgata gctgttaagc ccttccatcg gtaaataaag cgaagcgcca tggggctgcg
     9601 cattcaggcg cgcttccgcc tccgccacgg ctttcagttg tggaataatt ccgtcttcgt
     9661 tgtagtacag accgatactt aaattcactt tgtcgctgcg agggtcttct ttaaaacgct
     9721 ccataagcgt aagaatcgga tcgccagcgt aggcgtcaac tttttgaaac acgcgatggt
     9781 tctccaggtt tacgggcagg tggttaaaac acaataaacc ggaagaaggc gaagatcgag
     9841 tggatgttca ggagcgaacg gcaattagca acagagtgag actcatgaca aacgaacatc
     9901 cgccagaggc acgaccttta taagagcatg aaagaacatc aacttattga atttttagga
     9961 ttttattggc cggataaggc attcacgccg catccggcac agacaatcaa atattacaga
    10021 acgattaatc cacgtatttc atcgcgaccc ttgaagtcag gcgcgtaata agttcgtaag
    10081 cgctcacttt tgtcatttca gcgatacgtt ctacgggcaa accttcgccc cataaaatga
    10141 ccggatcccc ggctttgtcc tgcgcctgtg gacctaagtc tacgcagatc atatccatcg
    10201 ccacgcgtcc gacaatcggt acttcgcgac cgttcaccag cactggcgta ccggatggag
    10261 cggcgcgcgg ataaccatcg ccatagccca tcgcgactac gccaagacgg gtatcacgtt
    10321 cgcttaccca ggttccacca taaccaacag gctctccggc tttatgctca cgcacggcaa
    10381 tcaggctgga ggttagtgac atcactggct gacagccaaa atcggcaccg atggagcgat
    10441 cttccagcgg cgagacgcca taaagaatga tgcccgggcg cacccagtca aaatgcgatt
    10501 gtggccacag cagaatgcca cccgacgcgg caatggaacg ttgaccaggt ttgccttcgc
    10561 aaaaggtatt aaagatagcg agttgtttct cggttgcgcc acattttggt tcatccgcgc
    10621 gcgcaaaatg gctgacgata ttcaccggct gacgaacgtt tttgcactgt gtcaggcgat
    10681 gataaaacgc ctcagcctgt tccggcctta cgcccagacg gtgcataccg gtatcgagtt
    10741 tcatccagac ggtaaccggc tcgtccaggc tagcctcttc cagcgcagcc agctgttctt
    10801 cgttatgcac ggcggtatga aaatgttgcg cagaaatcgt cggcagatct ctggcatcaa
    10861 aaaagccttc gagtaacagt acaggtttgg tgattccccc cgcacgcagt cgcagagctt
    10921 cttcgagacg ggctacgcca aaggcgtcag catcggggag cgttcgcgcg gtctcaagaa
    10981 gaccgtgacc ataagcgttc gctttcacca ccgcaaccat tttactggca ggggccagtt
    11041 cacgaagacg ttgcaggttg tgtcgcagag cgcggcggtt aatcacaaca gttgccgctt
    11101 gcatttgtgt tccttgataa gtgtttgctt taattaccta attcataaaa taattattat
    11161 tcgtcgtcgt actgcggccc cgcatagttg tcgaagcgcg accattgacc gttaaaggtc
    11221 aggcgtaccg tcccgattgg gccgttacgt tgtttaccga taataatttc cgcgatgcct
    11281 tttaaatcac tgttttcgtg atacacctca tcacgataga taaacataat caagtccgca
    11341 tcctgctcga tagagccaga ttcacgcagg tcggagttga ccgggcgttt gtcggcacgt
    11401 tgttccagag aacggttcaa ctgggacagc gccaccaccg gcacgttcag ttctttcgcc
    11461 agtgctttca gcgagcgaga gatttctgca atttccagcg tacggttatc ggaaagcgcc
    11521 ggtacgcgca tcagttgcag gtagtcgatc atgataagcc cgatgccgcc gtgttcacgg
    11581 gcaatacggc gtgcgcggga acgcacttcc gttggcgtca ggccggagga gtcatcgata
    11641 tagatattgc gtttttcgag caaaataccc atggtgccgg aaatgcgcgc ccagtcttca
    11701 tcatcgagct gcccggtacg gattttagtc tggtcaacgc gcgacagcga cgccagagaa
    11761 cgcatcataa tctgttctga aggcatctcc agcgagaaga taagtactgg tttatcctgc
    11821 aacatcgccg cgttttcgac gaggttcatc gcaaatgttg ttttacccat cgacggacgc
    11881 gcggcgacga tgatcaaatc cgacggctgc aagccagcgg tttttttgtt gagatcgtca
    11941 taaccggtgt ttactccggt aacgccatcg tgtggctgct gaaacaactg ctcaatacgc
    12001 gccacggttg cgtcgagcac atcggcgatg ttcttcggcc cttcgtcttt gtttgcacga
    12061 ctttcggcaa ttttaaagac gcgggattca gcaaggtcca gcagatcttc gctggtacgc
    12121 ccctgcggat caaaaccggc ttcagcaatc tcattcgcaa ccgagatcat ctcacggaca
    12181 acggcacgtt cacgcacgat gtcagcataa gcactgatgt tcgccgcact tggcgtattt
    12241 tttgacagct ctgccagata agcaaaacca ccgacgctat cgagttgccc ctggcgttcc
    12301 agcgattccg caagggtaat cagatcgata ggactaccgc tttcctgcaa acgcgccatt
    12361 tcagtaaaga tatggcggtg tgggcgggtg taaaaatcgt ctgccaccac acgctcggct
    12421 acatcgtccc agcgttcgtt atccagcatt aaaccgccca acaccgactg ctccgcttcg
    12481 atcgagtgcg gaggcacttt cagcccggca acttgtggat cgcgttcgcg gggttcagcc
    12541 tgctgtttgt tgaagggttt atttcctgcc atagtgaatg gagttaccga gataaagaat
    12601 gggtcgaaac tttaccatat gaagcagacc ctgacgatac gttctggagg acacatggca
    12661 acacgaattg aatttcacaa gcacggtggc ccggaagtac ttcaagccgt agagttcact
    12721 cctgccgatc cggcagagaa tgaaatccag gtcgaaaata aagccatcgg catcaatttt
    12781 atcgacacgt atatccgcag cggcctttac ccgccgccat cactacccag cggattaggt
    12841 accgaagcgg caggcatcgt gagtaaagtc ggcagtggtg taaagcatat taaggcaggc
    12901 gatcgtgtag tctatgcgca gtcggcgtta ggcgcttaca gctctgtgca taacattaat
    12961 gcggataaag cggcgattct gcctgcggca atttcttttg agcaagctgc ggcatccttc
    13021 ctgaaaggct taacggttta ttatctgctg cgcaaaacct atgaaattaa acccgatgaa
    13081 cagttcctgt tccacgcagc ggctggcggc gttggcttaa ttgcctgcca gtgggcaaaa
    13141 gccctgggcg cgaaacttat cggcaccgta ggaaccgcgc aaaaagcgca gagcgcgcta
    13201 aaagcgggcg cgtggcaggt tattaactat cgtgaagagg atctggtcga gcggttaaaa
    13261 gagatcaccg gcggtaagaa agtgcgcgtg gtgtacgatt ccgtgggcag agacacctgg
    13321 gaacggtcgc tggattgcct gcaacgccgc ggcttaatgg tcagttttgg caactcatca
    13381 ggtgcggtta ccggtgtgaa cttaggcatt ctcaatcaaa aaggctcgtt gtatgtgaca
    13441 cgcccttccc tgcaaggcta tatcaccacg cgggaggaat taaccgaggc cagtaatgaa
    13501 ctgttctctt tgattgccag cggtgtgatt aaggtcgatg tcgccgagca gcagaaatat
    13561 ccgctgaagg atgcgcagcg tgcgcatgag attctggaaa gccgggcgac gcaaggttcc
    13621 agcctgttga ttccataaaa gaaatagggc ttccacctgg gaagcccttt ctttttatag
    13681 ttcggctgta tgtagggtac agcacgatga atctgttaga ggcgcaatag tgacagattt
    13741 gattatcaat tcctattttg ttctaaggat aaaaccttag gttgtgatca tccgcacaat
    13801 cccttagtaa cgccagcggt cataacgctg atatttcggc acttttggtg ctttaatcgc
    13861 cttaataacc cacaccaccg caatcgccag tagtaaccac ggcagcaact taatcatcaa
    13921 tgccagcata ccgccgagga acataatggc cgtcgccaca accagcgcgg cgataatgcc
    13981 cagcaacgaa acgccggtga ccatcagcat gacaaaaaag ccaatcacaa aaagtagttc
    14041 cagcatgatg ctctcccaaa tatgaaatct cttgctggca ttacaagaat catgccaaaa
    14101 ataatctatt gatttaacag caaaacgccc cgcgacggtg cgcagggcgt ggtgaatttg
    14161 actacttttt ggtgaaaagt taacgcttat ccgccaccag tttgagcgcg tgttccagca
    14221 cattaatgtc tgcacccgct ttatgggcat tttcacttaa ataacgccgc cactgccgcg
    14281 cgccaggaat accctggaac aagcccaaca tatgccgggt aatatggccg agatacgtcc
    14341 cctggctgag ttcacgctca atgtacggat acatggcgcg cactaccgcc accggatcgg
    14401 catcggtatc cgaggaacca aagatctccc ggtctaccgc cgccagaata cccggattct
    14461 gatacgcctc gcgcccgacc atcacgccat ccatatgttg caggtgtgct ttggcctctt
    14521 ccagcgactt gataccaccg ttaatcgaca ttgtcagatg cggaaagtca cgcttcagtt
    14581 gatacacacg cggataatcg agcggcggga tttcacggtt ttctttcggg cttaaccccg
    14641 aaagccaggc tttacgtgcg tggatgataa acatctcaca ctcgcctttg ccggaaacgg
    14701 tgttgatgaa atcgcagaga aattcatagc tgtcctggtc atcgatgcca atacgcgttt
    14761 tcaccgtcac tggaatcgac accacatcgc gcatcgcttt cacgcagtcg gcaaccagct
    14821 gcgcattacc catcagacac gcaccaaaca tgccgttctg cacccggtca gacgggcagc
    14881 cgacattcag gttgatctca tcatatccac gcgcttctgc cagcttcgca cactgtgcca
    14941 gcgccgccgg atcgctaccg ccgagttgca acgctaccgg atgttcttct tcactgtacg
    15001 ccaggtaatc acctttaccg tgaataatcg cccctgtggt caccatttcg gtatacagca
    15061 acgtattgcg ggaaagcaga cgcaagaaat agcggcaatg tctgtccgtc cagtcgagca
    15121 ttggtgcaac gctaaaccga ccactccagt gaacgtcagt tttttcaggc attgcgctgg
    15181 tttggttgat tttttgcatt tcagaattac cgtgcatttt caaatgtaga gattatttta
    15241 tcgatatatc atggggttat gttattcagc atcactattc aggaggctca atagcggggt
    15301 actataccat aacaacagga agcgcctgtc tcattgcaaa agaaaattga gatcctctca
    15361 aggcatgaag ctctcacgaa gtgatggaaa taatcttatt agccgttaac tttgttaagg
    15421 ccaatgataa acaatccagg ttcgacgata aataaaaaat cacacattaa actctggtga
    15481 tatatctccc tgctaatagc attgatagag aaaaaagaac ccaataaata ttgggtcctt
    15541 ttatataatg ccttccatac tatcgaagaa cttcacatat tatttctccg atttaacccc
    15601 gaacaaatca taaattaatt tagaagtgtc tgtaagtatt ttaatctctt cctttgaggt
    15661 tgggtcaaac gacttcgcga agctaattaa ttgtggtgca gcatctcgca ttttgcttaa
    15721 aatatcagga tcgagcgttc cttcattcac cagatgtgac atcttatcca gatagccatc
    15781 aaaattcatt tctcccccat cagccagacg cttcatctct cccaggtact tcttcatatc
    15841 acgttgagat aatttttcaa actgagcttt caggtagctc tcattatttt cattaatata
    15901 cactgtttct gataaatctg cgtaggcact agaatatgaa agtgaaatta acagtgaagc
    15961 aaataatttt aagctgttgt catcatgttc ctggcaggca ttaacaaacg ttaaaaaacc
    16021 agaacctatt gcttgaaaat gtatattcgc taaaggttca ttatccttag attcctcata
    16081 aaatagctga accgtggagg gaaggccatt cttagtttct acatcaaaag tacattgttc
    16141 aatgggagtc gcatctttat tatccccagg attacatata ctattaatcg cgaaatgaaa
    16201 aatagcccgg tcaacaactg atgcaataaa gtcacgatcg tttaaatcct catcagtgca
    16261 atcgtcaata tacttattga cccacatttt gagatcattt atttgctcaa ttaaaaacac
    16321 atttttttac gtgcatgcca taaattatgc gtgattcgtt gaataaacaa atctattttt
    16381 gcaataatta tatcccgatg acgcagcgct gcataatcaa tagatttacc atatatatta
    16441 tttaatttac ctttgattga acctctatta tctaatttat ctttgattgc atcttcatta
    16501 tcgaattgat ttgaattgag tgttttatta aagtatccag ttgcaccggg tattatcttc
    16561 aacatgattc atctaccttt tttgtcgtac cgtgtcctcc ttataattct tttgccatct
    16621 ggtcactatc aaaaatcgct cataatttct gaactacagg tatgcgttta aacccagtta
    16681 aataaatagc gagttaactt gaagcaattt atttaaaaat gaattattaa ttattatcgt
    16741 gttgataatg gtacgacaag gcaacataac accttaccta caaaacagaa aatcaacatt
    16801 acggcaacaa taagggttct cggtgttttt cctggaacat ggtaaagtaa ggacattctt
    16861 aacccccact ttgaggtgcc cgatggaaaa gaccacaacg caggagttat tagcgcaggc
    16921 tgaaaaaatc tgcgcgcagc gtaatgtgcg cctgacccca cagcgcctgg aagtgttgcg
    16981 cctgatgagt ctgcaggatg gcgctatcag cgcttatgat ctgcttgatt tactgcgcga
    17041 agctgaaccg caagccaagc cgccaacggt ttatcgcgcg ctggattttc tgcttgagca
    17101 aggttttgtg cataaggtgg aatccaccaa cagttatgtg ctctgtcatc tgttcgacca
    17161 gcccacgcat acgtcagcca tgtttatatg cgatcgctgc ggcgcggtga aagaagagtg
    17221 tgcagaaggc gtggaagaca ttatgcatac gctggcggca aaaatggggt ttgccctgcg
    17281 gcataatgtg attgaagcac atgggctctg tgcggcatgc gtggaagtgg aagcgtgtcg
    17341 tcatcctgaa cagtgccagc atgatcactc tgtgcaggtg aaaaagaaac cgcgttaaga
    17401 gggcgtacat ccttgtacac gtcgggcagg agggattaat taccagcgat attcattgcg
    17461 ggtttcccaa tccacgacct ctttttctgc ctgatctttc tgataaccat aacgttcctg
    17521 gattttaccg accagttgat cacgtttacc ttcaatgatc gtcatatcat catcggtcag
    17581 tttgccccat tgctctttca ctttaccttt aaactgtttc cagttaccgc cggcttcatc
    17641 tttattcata atcaagacct catcgttagg ttgtgaatga gagtacgttc acttttcttc
    17701 tgaacgtgag attaagtgta gtcagcaatt tggcttagga taatttattc agaattttta
    17761 accgtcacgt tgcggcaaac caggtaccgt tacgccagtg acgccgccag atagccgcca
    17821 gagaaagccc gcgcaacgcc agaaagacgg ttagtgccag ccacaaacca tgattaccca
    17881 gccacggcag cgtaaggagc gtcagcgcaa aacctgcggc ggccaccgcc atactgttac
    17941 gcatttcggc ggcacgcgtt gcgcctataa acatgccgtc cagcagataa caccagacgc
    18001 caaccaacgg caaaatcacc tgccagataa gatagcggtc agccagctgc tgaatctggg
    18061 ttaacgacgt cagcaacgca atgatgtgtt cccctgccag caaataaacc accgaaaata
    18121 gtaacgctac gatccccgac tggcggcacg ctgcccgcca gacatccagc aactggctac
    18181 cgtcgcgcgc accgtatgcc tgaccggaat gtgcttcaac cgcgtaggca aaaccatcca
    18241 gcgcataggc ggtaaaggtg agtagcgtca tcagaaccgc gtttacagcg ataatgtcac
    18301 tccccagtcg cgcgccaagt acggtgatcg cgccgaaaca gagttgcaac aacagcgaac
    18361 gcagcatgat atcgcggtta agcgccagca agcgacggaa gtttcctcgc caggcagttt
    18421 tcgacatttc gccggaaatt ccgcgtagtt tgaggatttt acgcaccatt agcagaccaa
    18481 tcagcaatgt tgcatattcc gcaataaccg tcgccagagc cgcgccctgc acgttcatat
    18541 gcagccccat cactagccag acatccagca caatgttgag gatattgccg accactaaca
    18601 aaattactgg cgcacgggca tattgcacgc cgagtaacca accgagtaat accagattcg
    18661 ccagcgacgc cggtgcgctt aaccagcgga tttcaagaaa gcgccgcgcc tgttccagga
    18721 ctgcttcact accgccaaca atatgcagag ccagatcgat aatcggtgta cgcagcagcg
    18781 caattaacgc cccagccccc aacgccaaca gcaacggttg caccagcgca cgggctaatg
    18841 cctgaggatt tttggcacca taagcctgcg cagtcagccc ggtggtgctc atgcgtaaaa
    18901 acagcaacag cataaagaga aagctggtcg ccgttgcacc aaccgccacg ccgcccaaat
    18961 aaaccgggct atcaagatga ccaattaccg ccgtatcgac cagtcccagc aacggaacgg
    19021 tgatattgga gaaaatcatg ggtaaggcga gatgccagag tgctttatca gatgaagtga
    19081 ggaatgccat gcagacaagc ctgatgaaga gagatgaaaa acaaaccgcg acgcctggcg
    19141 gcatcgcggt ctcagagata tgttacagcc agtcgccgtt gcgaataacc ccaaccgcca
    19201 gcccttcaat ggtgaagctc tgctgacgaa ggtcaacgac aattggttta aactcgctat
    19261 tttctggcaa cagttcgact ttattaccct gttttttcag gcgcttaacg gtaacttcgt
    19321 catcaatacg tgcgacaacg acctgaccgt tacgtacatc ctgagtttta tgcactgcca
    19381 gcaagtcacc atccataatg ccgatatctt tcatcgacat cccgctgacg cgcagcagga
    19441 aatcagcatt cggcttgaac aaggaaggat cgacctgata atgaccttca atatgctgct
    19501 gcgccagaag cggttcaccg gcagccacac gacctaccag cggcaaccct tcttcctctt
    19561 cctgcaacag acgaatcccg cgtgatgcgc cggaaacaat ttcaataacg cctttgcgtg
    19621 ccagcgcctt cagatgttct tcagccgcgt ttggggaacg gaaccccaaa cgctgcgcga
    19681 tttccgcacg cgtcggcggc atacctgtct ggctgatgtg atcacggatg agatcaaaca
    19741 cctcttgttg cctggccgtt aacgctttca ttccgccccc tgggtgtata tacagttatg
    19801 ctgtgagtat atacagcaaa aggcgatttt ggaaccataa actgcacaat aaaccagaga
    19861 tttatcgaaa tctggaaggg ttatccaaaa tgcgaccata acaggatgca ccaggttatc
    19921 acggcgacga taatcgcaat cagcaccgcg gcggatccca tatctttagc gcgtccggaa
    19981 agctcatggt attcagagcc aattcggtca accactgctt cgatggcgct attgagaatt
    20041 tccacaatca tcaccagcat cacggagctg ataagcagca cgcgggtaat tgcgtccaca
    20101 tccagccagc aggcgatgac caccgccaac aataccgcta cgccttcctg acggaatgcc
    20161 gcttcgttga tccatgcagc gcgtaaacct ttccaggaat agccagcagc tttgataatt
    20221 cgggtgaatc cagtggtatt attggccatt gaaagaacct ttttacatta tgagcgtcaa
    20281 tatcagtgta ccgttaaatc cgatgattcg ggtttgcgta acgccgcgcg aaacatgagc
    20341 ggataccaca gaatttccca tgactttctg ctatccttgc cgcgcatttg cattattaac
    20401 cagaggcttt acatcgttta tgtccggctg gccacgaatt tactacaaat tactgaattt
    20461 accattaagc atcctggtaa aaagcaagtc tattccggca gatcctgccc cggaactggg
    20521 gctggatacc tctcgtccaa ttatgtacgt tttaccgtac aactcgaaag cagatttgct
    20581 gacgttacgc gcccagtgtc tggctcatga cttgcctgac ccgttagagc cgctagaaat
    20641 cgacggcacg ctactgccgc gctatgtgtt cattcacggc gggccgcgtg tattcaccta
    20701 ttacacgccg aaagaagagt ctattaagct gttccacgac tatctcgatt tgcaccgtag
    20761 caacccaaat ctggatgtgc agatggtgcc agtatcggtg atgtttggtc gcgcgccggg
    20821 gcgtgaaaaa ggcgaagtaa acccgccgct gcgtatgctt aacggcgtac agaaattttt
    20881 cgccgtactg tggctcggtc gcgacagttt tgtgcgtttc tcgccgtcag tttcgctgcg
    20941 ccgtatggcg gatgaacacg gcacggataa aactatcgca cagaaactgg cgcgcgtggc
    21001 gcgtatgcac tttgcccgtc aacgtctggc tgccgtaggc ccacgtctgc ctgctcgtca
    21061 ggatctgttt aataagctgc tcgcctcccg cgccattgcc aaagcggtag aagatgaagc
    21121 gcgcagcaaa aaaatctccc atgaaaaagc gcagcagaac gcgattgcgc tgatggaaga
    21181 gattgcggcg aatttctctt acgagatgat ccgcctgact gaccgcattc tgggcttcac
    21241 ctggaaccga ctttatcagg gcatcaacgt ccataacgcc gagcgcgttc gccagctggc
    21301 ccacgacggt catgagctgg tgtatgtgcc ttgccaccgc agtcacatgg actacctgct
    21361 actttcttac gtgctgtacc accaggggct ggtgccgccg catatcgccg ccgggatcaa
    21421 cctgaacttc tggccggccg ggccgatttt ccgccgtctg ggggcgttct ttattcgccg
    21481 tacttttaaa ggcaataaac tttattccac cgttttccgt gagtatctcg gcgaactgtt
    21541 cagccgtggt tattccgtcg agtacttcgt ggaaggcggt cgttcccgca cggggcgttt
    21601 gctggatccg aaaaccggta cgctgtcgat gaccattcag gcgatgctgc gtggcggcac
    21661 gcgtccgatt acgctgattc cgatctatat cggttatgag cacgtcatgg aagtgggtac
    21721 ttacgccaaa gaactgcgcg gcgcgacgaa agagaaagag agcctgccgc agatgctgcg
    21781 cggtttaagc aagctgcgta atctcggtca gggttacgtc aacttcggtg aaccaatgcc
    21841 gttgatgacc taccttaacc agcacgtacc agactggcgt gaatctatcg atcccatcga
    21901 agcggtgcgt ccggcctggt taacgccgac ggtcaataat attgctgccg atctgatggt
    21961 acgcattaac aacgcaggcg cggcaaacgc catgaacctg tgttgtactg cgcttctggc
    22021 atcacgtcag cgctcactca cccgcgagca gttaaccgag caactcaact gctacctgga
    22081 tctgatgcgc aacgtacctt actccacgga ctctaccgtt ccttcagcca gcgccagcga
    22141 gcttatcgat cacgcgctgc aaatgaacaa gtttgaagtc gagaaagaca ctatcggcga
    22201 catcatcatt ctgccgcgcg agcaagcggt gctgatgacc tactatcgca acaacattgc
    22261 gcatatgttg gtgctgcctt cgctgatggc ggcaattgtc acccagcatc gccacatctc
    22321 ccgcgacgta ttgatggagc acgtcaatgt gctttaccca atgctgaaag cggagctgtt
    22381 cctgcgctgg gatcgcgacg agttgccgga cgttattgat gcgctggcaa atgagatgca
    22441 acgtcagggg ctgattaccc tgcaagatga tgagttgcat atcaacccgg cgcattctcg
    22501 ccccctacag ctgctggccg caggcgcgcg cgaaacgctg caacgttatg ccatcacctt
    22561 ctggttgttg agtgccaacc cgtcgatcaa ccgcggtacg ctggagaaag agagccgcac
    22621 cgttgcgcaa cgtctctccg tgctgcacgg catcaacgcg ccggagttct tcgacaaggc
    22681 ggtgttcagt tctctggtgc tgacgctgcg tgatgaaggg tatatcagcg atagcggcga
    22741 cgccgaaccg gcagaaacga tgaaggttta tcagttgctg gcggagttga ttacatcaga
    22801 cgtgcgtttg acgattgaga gtgcgacgca gggcgaaggg taatcagaga gaattgccgg
    22861 atgcggcgaa aacgccttat ccggccttcc ataatctgca aattcaataa attgcgatgt
    22921 gttgtgtagg cctgagaagc gcagcgcatc aggcaaccca gaagaaagcc ggatgatcat
    22981 ccggcttttt tacatcatca gaaatgccag taactcattg ccagtcctaa aaacagtacc
    23041 agaccaacat agttattatt cataaatgct ttaaagcagg cttcacgctc gcggttggca
    23101 atcagttttt gttgataaac aaacagcgcg ccagccacca gaattgacca gtaatatccc
    23161 cagcctaagc catttaactc accgatgatc gccatcagtg ccagtacgcc aatctgcaaa
    23221 ataccaataa tcaatttatc gtattggccg aacaggattg cggtggattt aataccaatc
    23281 ttcacatcat cgtcacggtc aaccattgca tactgcgtgt cgtaagccac cgcccagaga
    23341 atattggcga ggaacattaa ccagcaactc aatggcaccg actcactcac agcggcaaaa
    23401 gccattggaa tcgaccagcc aaacgccgcg cccagcacca cttgcgggag atgggtatac
    23461 cgcttcataa acgggtacac ccacgccaac gccagagcgg caatcgacaa cagaatggtc
    23521 atcgtattca gcgtcagcac cagtaaaaat gaaatcaata ccagcactac aaacagcgcg
    23581 cgcgcctctt tctccgttac cgcgccgctg ggaagcggtc ggttcgccgt gcgcttaaca
    23641 tgaccatcaa acttgcggtc agcataatca ttcaccacac atccggcagc gcgcatcagc
    23701 cagacacccg cgacaaacac cgccaggatc cagagctggg gaacgcccgg tgtcgccacc
    23761 cacaacgccc ataatgttgg ccagagcagc agtaacgcgc caattggctt atccgtacgc
    23821 attaagcgat gaaacgccag cagcttattc tgcgtcagac tccactccat attttttcct
    23881 cttagtacaa cggtgacgcc ggtaaaaaca gttctgttag caacagcggt ttaccgctta
    23941 atcgcaggcg ggaacgtcgc ccccacagtc cggcatcacg gcctatctca ataaagtccc
    24001 gggttaatgt cgatgatgtg aacagatagc gtcctagcgg cgttttaccc aatttttgta
    24061 acgccagctc cggcccgctt aacgttgaca caggaacgac ggtacgaccg gcaagccacg
    24121 gttcaccatc ggcacataac aaaatttcac gtaaccagta acgagactct ttcggcagca
    24181 gcggcagttc ttcggggatt tcattctgct cgacaaaccc ttcgcggatc atcgtcacgc
    24241 tcaccgtttt tccctgctgt tcaaaacgtt ttgtcatgga atcttccagc aacagccagt
    24301 cgagcagttg cgaatccagg gcagggatct ctttaaaata gcgcagcgca cgcagttgcg
    24361 ttaacgcggg gtgtgacatg ccgaactctc cgttacataa cgtaaaggca ttgtatcgca
    24421 gaaaagtgtt tacgggagat gtaaagcaaa ttaagtgatc taatgcgcaa caaaaacgca
    24481 acaatcgcat gaagtggtaa aacgctgttg aaccttacgc tttgccgatg cccgtgctgg
    24541 cgagggcaac cgtaggggca tcaccgccca tgatgccagg aacggtgatg ttttccttta
    24601 cttaaatacc tatattcttt atacgagtag ctcgccaact cgcagtaaaa attgggaaaa
    24661 gcgcatattt accatgagat ttggagctaa aaagtgtttc ctgagtaaga ttaataggca
    24721 acatcttatg tctggattta tagatacacg tggaattcca attggtatct tttgtaggat
    24781 tcaacatat
//
LOCUS       DAJTYZ010000055        24681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000055 DAJTYZ010000000
VERSION     DAJTYZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24681
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.055"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(130..1779)
                     /gene="actP"
                     /locus_tag="OO596_002116"
     CDS             complement(130..1779)
                     /gene="actP"
                     /locus_tag="OO596_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6717971.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1776..2089)
                     /locus_tag="OO596_002117"
                     /pseudo
     CDS             complement(1776..2089)
                     /locus_tag="OO596_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2289..4247)
                     /gene="acs"
                     /locus_tag="OO596_002118"
     CDS             complement(2289..4247)
                     /gene="acs"
                     /locus_tag="OO596_002118"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR6717972.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4641..6077
                     /gene="nrfA"
                     /locus_tag="OO596_002119"
     CDS             4641..6077
                     /gene="nrfA"
                     /locus_tag="OO596_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6717973.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6122..6688
                     /gene="nrfB"
                     /locus_tag="OO596_002120"
     CDS             6122..6688
                     /gene="nrfB"
                     /locus_tag="OO596_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6717974.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6685..7356
                     /gene="nrfC"
                     /locus_tag="OO596_002121"
     CDS             6685..7356
                     /gene="nrfC"
                     /locus_tag="OO596_002121"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6717975.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7353..8309
                     /gene="nrfD"
                     /locus_tag="OO596_002122"
     CDS             7353..8309
                     /gene="nrfD"
                     /locus_tag="OO596_002122"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6717976.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8329..10047
                     /locus_tag="OO596_002123"
     CDS             8329..10047
                     /locus_tag="OO596_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6717977.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10040..10423
                     /gene="nrfF"
                     /locus_tag="OO596_002124"
     CDS             10040..10423
                     /gene="nrfF"
                     /locus_tag="OO596_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6717978.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10420..11016
                     /gene="nrfG"
                     /locus_tag="OO596_002125"
     CDS             10420..11016
                     /gene="nrfG"
                     /locus_tag="OO596_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6717979.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11358..12671
                     /gene="gltP"
                     /locus_tag="OO596_002126"
     CDS             11358..12671
                     /gene="gltP"
                     /locus_tag="OO596_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6717980.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12750..13439)
                     /locus_tag="OO596_002127"
     CDS             complement(12750..13439)
                     /locus_tag="OO596_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6717981.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13533..15680)
                     /gene="fdhF"
                     /locus_tag="OO596_002128"
     CDS             complement(13533..15680)
                     /gene="fdhF"
                     /locus_tag="OO596_002128"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15261..15263),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6717982.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15878..17344)
                     /gene="mdtP"
                     /locus_tag="OO596_002129"
     CDS             complement(15878..17344)
                     /gene="mdtP"
                     /locus_tag="OO596_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6717983.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17341..19392)
                     /gene="mdtO"
                     /locus_tag="OO596_002130"
     CDS             complement(17341..19392)
                     /gene="mdtO"
                     /locus_tag="OO596_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6717984.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19392..20423)
                     /gene="mdtN"
                     /locus_tag="OO596_002131"
     CDS             complement(19392..20423)
                     /gene="mdtN"
                     /locus_tag="OO596_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6717985.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20442..20717)
                     /locus_tag="OO596_002132"
     CDS             complement(20442..20717)
                     /locus_tag="OO596_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6717986.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20926..22911)
                     /gene="yjcS"
                     /locus_tag="OO596_002133"
     CDS             complement(20926..22911)
                     /gene="yjcS"
                     /locus_tag="OO596_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR6717987.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23164..23481
                     /locus_tag="OO596_002134"
     CDS             23164..23481
                     /locus_tag="OO596_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR6717988.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23550..23879
                     /gene="yjdP"
                     /locus_tag="OO596_002135"
     CDS             23550..23879
                     /gene="yjdP"
                     /locus_tag="OO596_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR6717989.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(24026..>24681)
                     /gene="phnP"
                     /locus_tag="OO596_002136"
     CDS             complement(24026..>24681)
                     /gene="phnP"
                     /locus_tag="OO596_002136"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR6717990.1"
                     /translation="RRQPCSGVVKFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHM
                     DHVQGLFPLRWGVGDPIPIYGPPDEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVT
                     PLPLNHSKLTFGYLLETAHSRVAWLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRAD
                     APRNHCDLNTVLALNQVIRSPRVILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
BASE COUNT         5597 a         6467 c         6727 g         5890 t
ORIGIN      
        1 atcattttct acgactgtca aaaatcgctc attttttaat gagtttattt gtttaatatt
       61 ttgggaaaag gtgatgcatg ctggagagga agagtattcc ccggtcaggc gaccggggaa
      121 ggggagagat taatgtgcgc ggccttgctc aacgccaaag ccggtctggg agcggataaa
      181 ctgcgcgcgg aacagttcac gctcgcgcgc cccttccgct gagttatcgg ttgccgagaa
      241 gaaccagatg ccgaggaatg ccacggtgat agagaacagc gccgggtatt catacgggaa
      301 gatggctttt tcgtgaccaa ggatctgtac ccaaatcgtc gggccgagga tcatcagtac
      361 tactgcggta atcagcccca gccagccacc catcatcgcg ccacgcgtgg tcagtttcga
      421 ccagtacata gaaagcagaa tgatcgggaa gttacagctc gccgcgatgg caaacgccag
      481 ccccaccata aaggcgatgt tctgattctc aaacagcacg ccgaggataa tcgcaatcac
      541 gccgaggatc agtacggtga tttttgatac ccgcagctct tcacgttcgg tcgcgccttt
      601 tttgaagacg ttagcgtaca agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc
      661 cgcaaccacc gcgaggatag tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt
      721 gccgcccact gcattcgcca ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc
      781 cgccgcgtct ttatattccg gattcgcacc aaccagcatg atcgcgccga agccgataat
      841 aaaggtcaga atatagaagt agcccataaa cccggtggcg tagaacacgc tcttacgtgc
      901 ttcgcgggca tcgctgactg taaagaagcg catcagaatg tgcggcaagc ccgccgtacc
      961 aaacatcagt cccagaccca gagagagtgc ggagatcgga tctttcacca gcccgcccgg
     1021 cttcatgatg tcgacacctt tcgggtgtac cgccatcgct tcactgaaca gattgttgaa
     1081 gctaaagccg acgtgtttca tcaccataaa ggccataaag ctggcaccga acagcaacag
     1141 cacggctttg ataatctgta cccaagtggt cgccagcatg ccgccgaaca ggacgtacat
     1201 catcatcagc acgccgacca gcaccaccgc aatgtgatag ttaaggccaa acagcagctc
     1261 gatcagttta cctgcgccca ccatctgggc gataaggtaa agcgccacca ccaccagaga
     1321 accacaggcc gaaagaatac gaatcggccc ttgtttcaga cggtaagagg ccacatcggc
     1381 aaaggtgtag cgccccaggt tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca
     1441 gcccaccagg aagcccagcg agtaaattaa gccgtcatag ccggaggtaa acaccagcgc
     1501 ggagatcccc aagaatgagg cggcggacat atagtccccg gcaatcgcca gcccgttctg
     1561 gaagccagtg atattgccgc ctgcggtgta gtagtcgcta cgagaacgta cgagttttga
     1621 tgcccagtag gtaatgccga gcgtaaacac gacgaaaatc aggaacataa taatcgcctg
     1681 ccagttcgtt ggctggcgct ctacggcccc gctaatagca tccgcggcgt tagctgcgaa
     1741 agggagtgtg gcggcaagcg ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg
     1801 acttcattat taagacggtc gaattcgccg ttcgcccgcc agatgtagat accggtgaga
     1861 acaaaggaga tcacaatcac tccaacacca attggaatac cgcgtgtgac gctggtgttc
     1921 ggattcagcg gcgtgccagc cagccgggcg cgaaggcgat cagtaaaata aagccgatat
     1981 aaactgccag cataataatc gacaggatgg tggcaaaccg ttgccgtttt tcgactaact
     2041 ccctgaaatg cgcattgtct tctatccgct gataaatagt gccattcatc acagattctc
     2101 cagaggtaat gtagggattg ttttaattcc cgctccctta tgggagaagg ttaacgctcg
     2161 ggtaaccctt gccgaatgta ggccggataa ggcgtttacg ccgcatccgg caatcaatgc
     2221 ctgatgcgac gctgtcgcgt cttatcaggc ctacaaaccg ttaccgactc gcatccggca
     2281 attgtgggtt acgatggcat cgcgatagcc tgcttctctt caagcagctt ctcgactacg
     2341 ccaggatcgg caagcgtcga ggtatcgccc aggttgctgg tatcgcccgc cgcaattttg
     2401 cgcagaatac ggcgcataat tttgccggag cgggttttag gcagggagtc ggtccagtgc
     2461 agcacgtctg gcgtcgccag cgggccaatc tctttacgca cccagttgcg gacttctgcg
     2521 tacagttctg gtgacggttc ctccccgtga ttaagcgtga cgtaggcgta gatcgcctga
     2581 cctttaatat tgtgcggaat acctactacg gcggcttcgg caatcttcgg atgcgccacc
     2641 agcgccgact caatctctgc cgtccccaga cggtgaccgg agacgttcag cacgtcgtcc
     2701 acacgcccgg tgatccagta atagccatct tcatcgcgac gcgcgccgtc gccgctgaaa
     2761 tacatatttt tgaaggtgga gaagtaggtc tgttcaaaac gttcgtgatc gccaaacagc
     2821 gtacgcgcct gacccggcca ggagtcggtg atcaccaggc tgccttcggt agcgccttcc
     2881 agcgggttac cttcgttatc gaccagcgcc ggttgcacgc cgaagaacgg acgtgttgcc
     2941 gaaccggctt tcagctcggt agcgccaggc agcggcgtga tcatgaaacc gccagtttcg
     3001 gtctgccacc aggtatcgac caccggacat ttctcgttgc cgattttttt ccagtaccac
     3061 tcccacgctt ccgggttaat tggctcgccc acggaaccga gaatgcgcag cgacgaacgg
     3121 tcggtaccct cgatcgcttt atcgccttcc gccatcagcg cgcggatcgc cgtgggtgcg
     3181 gtatagagaa tattgacctg atgcttgtcc accacctgcg ccatacgggc aggcgtcggc
     3241 cagttgggta cgccttcaaa catcagcgtg gtcgcaccgc aggccagcgg gccgtacagc
     3301 aagtaactgt gtccggtcac ccagcccaca tcggcggtgc accagtagat atcacccgga
     3361 tgataatcaa agacatattt aaaggtcagc gccgcgtaca ccagataacc gccggtagta
     3421 tgcagcacac cttttggctt accggtagaa ccggaggtgt agagaataaa cagcggatct
     3481 tcggcgttca tcttttccgc ctggtgctga tcgctcgctt gctcaaccag gtcgtgccac
     3541 cacaggtcgc gcccttcctg ccagtcaatt ttcccgccag tacgcttcag taccaccaca
     3601 tgctctacgc tggtgacgtt cgggtttttc agcgcgtcat caacgttttt cttcagcgga
     3661 atactgcgcc cggcacgcac accttcgtcg gaagtgatca ccagtcgtga gttggaatca
     3721 ataatgcgcc cggcaacggc ttccggcgag aagccgccga aaatcaccga atgcaccgcg
     3781 ccaatgcggg cgcaggccag catcgcaacc gcggcttccg gcaccatcgg catataaatc
     3841 gccaccacat cacctttttt aatgcccagc tcgagcaggg tattggcgaa gcggcagacg
     3901 tcgcggtgca gctctttata gctgatatgt ttgctctggc tggcgtcgtc gccttcccag
     3961 atgatggcgg tacgatcgcc gttttcttgc agatggcggt caaggcagtt tgccgccaga
     4021 ttcagcgtgc cgtcctcgta ccatttaatg gacacattac cgggggcaaa ggaggtgttt
     4081 ttcaccttct ggtaaggttt gatccagtca agaatttttc cctgttcgcc ccagaaggta
     4141 tcaggtacgt taatagattg ttgatacatc gcctcgtact gctgagggtt tatcaggcaa
     4201 cggtctgcga tgttggcagg aatggtgtgt ttgtgaattt ggctcatgct tttgttctcc
     4261 ttgtagggtg ttaataatat gtggcataag cgttaaatgt aggggtattg gcagttttgt
     4321 ttagtatttg ggcgacagat cacgcaaaag tagaattgtg caaataaacg gcagggtaat
     4381 ttttgaaggt cagcaacaaa agttgattaa ttctttgagg aacatgcagt tatgcattgc
     4441 tgttgaaaaa gaggaagata ctaactaact ctaaagtggt attttacatg cacttacaat
     4501 tgattaaaga caacattcac agtgtggtta tttgttacac ataggggcga gcaatgtcat
     4561 gacagtgtag gtgcggttac tgtcgtgaaa aacaataaaa accgccattg taacaatggc
     4621 gcaattcgga tgaagcccct atgacaagga taaaaataaa cgcacgccgt atcttcagct
     4681 tattgattcc ttttttcttt ttcacttctg ttcacgctga acaaacggca gctcccgcaa
     4741 aacctgtaac tgtggaagcg aagaatgaaa cctttgcccc gcagcatccc gatcaatatc
     4801 tctcctggaa agccacctcg gaacagtcag agcgtgttga cgccctggcg gaagatccac
     4861 ggctggtgat cctgtgggcg gggtatccct tctcgcgcga ttacaacaag ccgcgtggac
     4921 atgcctttgc tgtgaccgat gtgcgtgaaa ccctgcgtac cggtgcgccg aaaaacgctg
     4981 aagatggtcc gctaccgatg gcgtgctgga gttgtaaaag cccggatgtg gcgcgtctga
     5041 tccagaaaga cggcgaagat ggctacttcc acggtaagtg ggcgcgcggc ggcccggaaa
     5101 tcgtcaacaa cttaggttgt gccgactgcc ataacaccgc ctcaccagag ttcgccaaag
     5161 gcaaaccgga gttaaccctt tcccgtccgt atgcggctcg cgcgatggaa gccattggta
     5221 aaccttttga gaaagccgga cgtttcgacc agcaatcgat ggtttgcggt cagtgccatg
     5281 tggagtatta cttcgacggc aaaaacaaag cggttaaatt cccgtgggat gacggcatga
     5341 aagtcgaaaa tatggagcag tattacgaca aaattgcctt ctctgactgg actaactccc
     5401 tgtcgaaaac gccaatgctg aaagcgcagc acccggaata tgaaacctgg acagcgggca
     5461 ttcacggtaa aaacaacgtg acctgtatcg actgccatat gccaaaagtg cagaacgccg
     5521 aaggcaaact ctataccgac cataaaattg gtaatccgtt tgataacttc gcccagactt
     5581 gtgcgaactg ccatacccag gacaaagctg ccttgcaaaa agtggtcgcg gaacgtaagc
     5641 agtcgattaa cgacctgaaa atcaaggttg aagatcaact ggttcacgct cacttcgaag
     5701 cgaaagcagc gctggatgca ggcgcgacgg aagctgaaat gaagccaatt caggacgata
     5761 tccgtcatgc ccagtggcgc tgggatctgg cgatcgcttc ccacggcatt catatgcacg
     5821 caccggaaga aggtttacgg atgctcggta cggcgatgga taaagcggcg gatgcacgca
     5881 ccaaactggc gcgcctgctg gcgaccaaag gcatcaccca tgaaatccag atcccggata
     5941 tctcaaccaa agagaaagcc cagcaggcca ttggcctgaa catggaacaa atcaaggccg
     6001 agaagcagga cttcatcaaa acggtgatcc cgcagtggga agaacaggca cgtaaaaacg
     6061 gtctgttaag ccaataaccc cgttccgcct cgcaaggggc ggaaaacaca atggagtgaa
     6121 tatgagcgta ttacgttcgt tattaactgc cggggtgctg gcgtcgggcc tgttgtggag
     6181 cctgaacgga attaccgcta cccctgcggc gcaggcatct gatgatcgct acgaagttac
     6241 ccagcagcgt aacccggatg ccgcctgtct ggactgtcat aaaccagata ccgaaggtat
     6301 gcatggcaaa catgcttccg ttatcaaccc gaataacaaa ctgccggtca cctgcaccaa
     6361 ctgtcacggc cagccatcac cgcaacaccg cgaaggggtg aaagatgtga tgcgctttaa
     6421 cgagccgatg tacaaggttg gagagcagaa cagcgtctgt atgtcctgtc atctgccgga
     6481 acagttgcaa aaagcgttct ggccgcacga tgtccacgta accaaagtgg cgtgcgccag
     6541 ctgccattcc ctgcatccac agcaagatac gatgcagacg ttaagtgaca aaggacggat
     6601 taagatttgc gtcgattgcc acagcgatca gcgcaccaat ccgaacttta acccggcgtc
     6661 tgttccgttg cttaaggagc agccatgacc tggtctcgtc gccagtttct caccggcgtc
     6721 ggcgtgctgg cagccgtcag cggcaccgca gggcgcgtgg tggcgaaaac gttgaatatc
     6781 aatggggtgc gttacggcat ggtgcatgac gagtcgttat gcatcggctg taccgcctgt
     6841 atggatgctt gtcgggaagt gaacaaggtg ccggaaggcg tctcgcgcct gacgattatt
     6901 cgtagcgagc cgcagggcga atttcctgat gtgaaatatc gcttcttccg taagtcttgc
     6961 cagcactgcg atcatgcgcc gtgcgttgac gtctgcccga ccggtgcgtc ttttcgtgat
     7021 gccgctagcg ggattgtcga tgttaatcct gatctctgcg tcggttgtca gtactgcatc
     7081 gccgcctgtc cgtaccgcgt gcgctttatc catccggtca cgaagacggc ggacaaatgc
     7141 gatttctgcc gtaaaaccaa tttgcaggcc ggtaagctgc ccgcgtgtgt tgaggcttgc
     7201 ccgacgaagg cgctgacgtt tggcaatctg gacgatccta acagtgagat ttcgcaactg
     7261 ctgcgccaga agcctactta ccgctacaag ctggcgctgg gaaccaaacc gaagctgtac
     7321 cgcgtaccgt ttaaatacgg ggaggtgagc caatgacgca gacttccgca tttcattttg
     7381 aatcgctggt gtgggactgg ccgattgcca tctacctgtt tttgattggt atttctgccg
     7441 gtctggtgac gctggccgtg ctgttacgtc gcttctaccc gcaggcgggc ggtgcagaca
     7501 gtacgttgct gcgcaccacg ctgattgtcg ggccgggcgc ggtgatcctc ggtctgttga
     7561 tcctcgtctt ccacctgaca agaccgtgga ccttctggaa gctgatgttc cactacagtt
     7621 ttacctcggt gatgtcgatg ggggtgatgc tgtttcagct ctacatggtg gtgctggtgc
     7681 tgtggctggc gaaaatcttt gaacatgatt tgcttgccct gcaacaacgc tggttgccga
     7741 agctggggat cgtgcaaaag gttctgagcc tgctgacgcc cgttcatcgc ggactggaaa
     7801 cgctgatgct ggtgttggcg gtgctgctgg gggcttatac cggctttctg ctgtcggcgc
     7861 tgaaatcgta tccgttcctc aataacccga tcctgccggt gctgttcctc ttctccggca
     7921 tctcgtccgg tgcggcggtg gcgctgatcg ccatggcgat acgccaacgc agtaacccgc
     7981 attccacgga agcgcacttt gttcaccgga tggaaatccc cgtggtatgg ggtgaaatct
     8041 tcctgctggt ggcgtttttt gtcggtctgg cgctgggcga tgacggtaaa gttcgtgcgc
     8101 tggtggcggc attaggtggc ggtttctgga cgtggtggtt ctggcttggt gtcgccgggc
     8161 tggggctgat tgtgccaatg ttgctcaaac cgtgggtcaa tcgcagttcc ggcattcctg
     8221 ccgtgctggc ggcgtgtggg gccagtctgg tcggcgtgtt gatgctgcgc tttttcattc
     8281 tctacgccgg acagttgacg gtggcgtaag ccagaaaaga ggtggtttct ggacgtattc
     8341 cttcctgaag tcggttttct ggcgttgttg ttaagtctcg gggtcaacgt gttgaccccg
     8401 ttgacggcct ttgcgggagt gcggttgcgc tggcctgcca tgatgcgact cacttgcatc
     8461 ggcattctgg cgcagttcgc gctcctgctg ctcgcctttg gcgtactgac gtattgtttt
     8521 ctcatcagcg atttctcggt catttatgtc gcgcaacata gctatagcct gctgtcgtgg
     8581 gaattcaagc tggcagcggt gtggggcggc catgaaggtt cgctgctgct ttgggtgctg
     8641 ctgctttccg cctggagcgc gctgtttgcc tggcattatc ggcagcaaac cgatccgcta
     8701 tttccgctga cgttagccgt tttatctctc atgctcgccg cactgctact gtttgtggtg
     8761 ctgtggtccg atcccttcgt gcggatattt ccaccagcaa tcgaaggccg cgatctcaat
     8821 ccgatgctgc aacatcccgg tcttatcttt catccaccgc tgctttacct tggctatggc
     8881 ggtttgatgg tagcggcgag cgtggcgctg gcgagtctac tgcgcggcga gtttgatgcg
     8941 gccagcgccc gaatttgctg gcgctgggcg ttacctggct ggagtgcatt aacggcgggg
     9001 atcatcctcg gttcctggtg ggcctactgc gaactcggct ggggcggctg gtggttctgg
     9061 gatccggtgg aaaatgcctc tttattaccc tggctttctg ccactgcgct gctgcacagt
     9121 ttatccctga cacgccagcg ggggattttt tgccactggt cgctgttgct ggcgatagtt
     9181 actctgatgc tgtcgctgct gggcacctta attgtccgtt ctggcattct ggtttcggtt
     9241 catgcgttcg cactggataa cgtccgcgcc gtgccgttgt tcagcctgtt tgcactgatt
     9301 agccttgcgt ctttggctct gtatggctgg cgagcgcggg acggtggccc ggcggtgcgt
     9361 ttttcggggt tatcgcggga aatgttaatc ctcgctacgc tgttgctgtt ttgcgcagtg
     9421 ctactgatcg tgctggtggg aacgctttat ccgatgattt acggtctgct aggctgggga
     9481 cgcctctccg ttggcgcgcc gtattttaac cgcgcgacgt taccgtttgg tctgttgatg
     9541 ctggtggtga ttgtgctggc gacgtttgtc tctggcaaac gcgcgcagct tccggcgctg
     9601 gtcgctcatg cgggcgtgct gttatttgcc gcagggatcg tggtttccag cgtcagccgt
     9661 caggagatca gcctgaattt acagccgggt cagcaggtga cgctggcagg atacaccttc
     9721 cgttttgagc gcctcgatct gcaagccaaa ggcaattaca ccagcgaaaa agcgatagtg
     9781 gcgctgtttg accatcagca acgcattggt gaattaacgc cggagcggcg tttttatgaa
     9841 gcacgccgtc agcaaatgat ggaaccgtca attcgctgga acggcatcca tgactggtat
     9901 gcggtcatgg gtgaaaaaac cggagcggat cgttacgctt ttcgtttgta tgtacaaagc
     9961 ggtgtgcgct ggatctgggg tggaggattg ttgatgattg cgggcgcatt gttaagcgga
    10021 tggcggggga agaagcgcga tgaataaagg gcttctcacg ctactgctgt tatttacctg
    10081 ttttgcgcgc gctcaggtcg tagacacctg gcaattcgcc aatccgcaac aacagcaaca
    10141 ggcgttaaat attgccagcc agttacgttg tccgcagtgc cagaatcaaa acttactcga
    10201 atccaatgcg ccggtggcgg tcagtatgcg ccatcaggtt tatagcatgg tgtcggaggg
    10261 gaagaacgaa gtcgaaatca ttggctggat gaccgaacgc tacggagatt ttgttcgcta
    10321 taacccaccg ttaacgggtc agacgctggt gttgtgggcg ctgccagtgg tgttgttatt
    10381 gctgatggca ctgatcctct ggcgagtgag ggctaagcga tgaaacagcc tcaaataccg
    10441 gtgaaaatgc tgacaacact cacgatattg atggtattcc tctgtgtcgg cagttatctg
    10501 ttaagcccaa aatggcaggc ggtacgtgcg gagtatcagc gtcagcgcga tccgctacat
    10561 cagtttgcca gccagcaaac cccggaagcg cagcttcagg cattgcagga taaaatccgc
    10621 gctaatccac aaaacagcga acaatgggcg ttactgggcg agtactatct gtggcaaaac
    10681 gattacagca attcgctgct ggcgtatcgt caggcgttgc aactacgtgg tgagaacgct
    10741 gaactgtatg cggcgctggc gacggtgctt tattaccagg ccagccagca tatgaccgcc
    10801 cagacttgcg caatgatcga caaagccctc gcgctggact ctaacgaaat caccgctctg
    10861 atgctgctgg cttccgatgc gtttatgcag gcgaattacg cgcaagccat cgaactatgg
    10921 caaaaagtga tggatctcaa ctcaccccgg atcaaccgga cacagctggt cgagtcgatt
    10981 aatatggcaa aactgttgca gcggagatcg gattaatcat ctggtgatga agattatgtc
    11041 attgagctgc ataaaaaata atcgaatgaa catatgccaa aaataatcac taatcagtat
    11101 tattgcagat taaacaaata aaaatctttc cataacaaat ggttattcat taatcctgcc
    11161 ataaactcgc gttattatgc attaatgcag cgaaaagctc tgttgttaaa gggttgcgca
    11221 acataccgcg caaatgatac tgatcataag cgttaaaaaa atctacaaac caacgcaaca
    11281 caattcatgc cctggcagta tgtcacgttc tcgcgtttct gaacggggaa cggcgctcca
    11341 ttgaggaagt tattctgatg aaaaatataa aattcagcct ggcctggcag attctgtttg
    11401 ctatggtgct gggcattctc ctgggaagct acctgcacta ccatagcgac agccgcgact
    11461 ggctggtcgt caatttgctc tctccggcgg gtgatatctt catccatctg atcaaaatga
    11521 ttgttgtgcc gattgtgatc tccacgctgg tggtgggtat cgcgggtgtt ggtgatgcca
    11581 aacagctcgg gcgtattggc gcgaaaacca ttatttactt cgaggtgatc accaccgtcg
    11641 ccatcatttt ggggatcact ctggcgaacg tcttccagcc cggtgccggg gtggatatgt
    11701 cgcagctggc gaccgtcgat atctcgaaat atcagagcac tacggaagcg gtacaaagca
    11761 gttcccacgg cattatgggc acgattttgt cgctggtgcc gacgaacatt gtggcgtcga
    11821 tggcgaaagg cgaaatgctg ccgatcatct ttttctcggt gctgtttggt ctggggcttt
    11881 cttccctgcc cgcgacgcat cgtgaaccgc tggtgaccgt gttccgctcc atctctgaaa
    11941 ccatgtttaa agtgactcac atggtgatgc gttatgcacc ggtgggtgtg tttgcgctga
    12001 ttgcggtgac ggtggctaac tttggtttct cttctctgtg gccactggcg aaactggtgc
    12061 tgctggtgca tttcgccatt ctgttcttcg cgctggtagt gctgggaatt gtggcgcgcc
    12121 tgtgcgggtt aagcgtctgg atcctgattc gtattctgaa agatgagctg attctggcgt
    12181 actccactgc cagctctgaa agcgtactgc cgcgaattat tgagaagatg gaagcctacg
    12241 gagcaccggc gtcgatcacc agcttcgtgg tgccgaccgg ttactctttt aaccttgatg
    12301 gttcgacgct gtatcaaagt attgccgcta ttttcatcgc tcagctgtat ggcattgacc
    12361 tgtccatctg gcaggaaatc attctggtgc tgacgctgat ggtgacctcg aaagggattg
    12421 ctggcgtgcc gggcgtgtcg tttgtggtgc tgctggcaac gctgggtagc gtaggtattc
    12481 cgctggaagg tctggcgttt attgctggtg ttgaccgtat cctcgacatg gcgcgtactg
    12541 cgctgaacgt ggtgggtaat gcgctggcgg tgctggtgat tgccaagtgg gaacacaaat
    12601 ttgaccgcaa aaaggcgctg gcttatgagc gtgaagtgct gggcaaattt gataaaactg
    12661 cggatcaata attgaagatt gccggggata tccacccggc aatgtgtgaa tgcctgatgc
    12721 gacgcttgcc gcgtcttatc aggcctacct taaccgttgg ttaatttttc aaactcttca
    12781 cacccggtat caaacccttc catacagctc aggttcaacc agtgcagcgc cttttgcttg
    12841 ttcttctcga taaaaccttc ttcaccgttt aagaacatca ttcccgccca gtactcggaa
    12901 taaccggttc gggaaattgc agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg
    12961 gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggca
    13021 gagtcgttct ccagatcttc cgaggcgttt tctaacagcg aaatagcctt tggataatcc
    13081 ggtttacccg cttgagtatt taccagaata tgcgccagcg ttacttcacc ttctttactg
    13141 cccgcttgcg ccgctttctc tgcaagaact tttgcttgtg gatagtccag actgaccgga
    13201 ttggtgattt tgatctgcgc cagcagcgcg caggcatcag catcaccgct gtcggcggct
    13261 ttctgtgccc aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga
    13321 aaatattgtg cgcgtcggtc cccggcctct gctgccttta aatactggct gccaggctcg
    13381 gagtcgttgg cgtgggcaaa gaatgtcaaa aacaacatca atgcgataat ttttttcatt
    13441 ttgataatac gattgggtag ggagtaacca gtataaagag ttcgaatgga taaaaaaaca
    13501 gcctccgaaa ggaggctgta gaaaggactg tattacgcca gtgccgcttc gcgcaggcga
    13561 gttttcaact tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg atcggcgatc
    13621 ggctcgacgc gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt
    13681 tcggtaacca gctcgttaca ggcaccaatc caccactggt aggtcatgta aatcgcccct
    13741 ttgttcggac gatcgctgac ctgcgcacgg gtgataattt tgcctttacg cgagtgcacc
    13801 caaaccaatg cctcatcttc aatacccaga cgtttggcgt cttcggtatt gatttgtgcg
    13861 tagccaggtt catcagccag cgccgccagt gccgcacagt taccggtcat cgaacggcaa
    13921 gagtagtgac caacttcacg caccgttgac agtaccatcg ggtactcgtc ggtgagtttg
    13981 tcgattggcg ctacccagtc gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac
    14041 ttctctttaa acagataaga agtcccctga tcggcatctg aagtatcgcg gcaaggccac
    14101 tgaatgaagc ccagttcgcc cattttctcg taagtcgcac cgtagaaatc cgggcacaga
    14161 tgacgcaact catcccagat ctcctgggtg ttgttgtagt gcatcggata acccatacgg
    14221 gtggcgattt cactgatgat ttgccagtcc gttttcagat cccatttcgg ttcaactgct
    14281 ttgaagaaac gctggaagcc acggtcagcc gcagtaaaca cgccttcatg ctcgccccac
    14341 gacgttgacg gcaaaatgac atccgccgcc gacgcggttt tggtcataaa gatgtcctga
    14401 acgataacca gttccagatc ttcaaaggct ttacgtactg ccgacagctc cgcgtcagtt
    14461 tgcagcggat cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc
    14521 ggcagctcgc tgatgcgata accggtatga gcaggcaggc tttccacgcc ccaggctttg
    14581 gcgaatttct cgcggttagc cggatctttc acgtactggt atcccggata cgtatccggc
    14641 agcgcgccca tatcgcaggc accctgcacg ttgttctgac cacgaaccgg gtttacaccc
    14701 gcatgcggct taccgaggtt accagtcagc atcgcgaggc tggtcagaga acgcacggtt
    14761 tccacgccct ggtagaactg ggttacaccc atgccccaca ggatggcggc gcttttcgcc
    14821 tgggcataca tccgtgccgc ctgacgaatc tcactggcgc tgacgccggt gatatcttca
    14881 accgactccg gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg
    14941 gaagcgacga acgctttgtc gtacagattt tcttcaataa tgacatggcc catcgcattc
    15001 aacagcgcga tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt
    15061 tcaattttgc gcggatcgca gacgataatt ttcgccccgt tacgtttagc gttaattacg
    15121 tgattcgcca cgattgggtg ggaatcagcc gggttgtacc cgaaaacgaa cactaaatcg
    15181 gtattatcaa tttcgttaat agcattgctc attgcgccat taccgaccga ttggtgcaga
    15241 cctgcaaccg atgggccgtg tcagacacga gcgcagcagt caacgttatt ggtaccaata
    15301 acggcgcgcg caaatttttg cattacatag ttggtttcgt tacccgtacc acgcgaggag
    15361 ccggtcgtct ggatggcatc cggaccgtac ttctctttga tggcgctcag gcgctcggta
    15421 acgtaattca gtgcctcatc ccaggaaaca ggttcgagtt tgccgccacg ctggcgacgg
    15481 atcatggggg ttttcaggcg cggggtcagg atctgggtat cgttaatgaa atcccagcca
    15541 taataaccct tcagacacag ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg
    15601 acgattttgc cgttatcgac gaccaggttg attttgcaac ctgatgcgca ataggggcaa
    15661 accgtgacga cttttttcat cggtctcgct ccagttaatc aaatcacgca tacgcgctct
    15721 cgactacagt atgcatcttt tatgccacat tttatttggg gtcattccct gatattacgg
    15781 gcactattta ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa
    15841 cgaaataccg cgtgacagcc atcacgcggc agacatttta ttttttctcg acgacgggag
    15901 ccgcctgata cccgccgccc agcgatttca tcaactgaat gctttggatc acccggcggc
    15961 tgtccagcat cagtaatgac atctcttcgg caagtactgg caaccgggct tcagtggcct
    16021 gtaagcggct ggttaagccg cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg
    16081 ttgcttccac gcgttcagcc tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc
    16141 cgttgacggc aacgtcacgc accgcgttca gtactgactg gttgtaacgt tcaatcatca
    16201 tgttgctggc ggcgcgcgtg ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg
    16261 gcaatttcag acccgggatg aagttgaact ggcgactggt ttttttgaat aaggtatcca
    16321 ggtggatggc atcaaggccg aaaaacgctt tgatatcaaa gctcgggtag aacagcgccc
    16381 gcgcggaatc cacctgatct aatgacgcct gaacatacca gcgcatggct tgcagatccg
    16441 ggcgtctggc gagcaactca taagagagtg tcgccggaat gccggtctgg actcgtggta
    16501 atgccaccgg tttgatctcc ggcatatcgc tggcccccgc gccaatcaat gcacgcagag
    16561 attctcgcgt ttcggtgatt tgccctttga cggcagcaat ttgtttatcg accgccagaa
    16621 tctgtgcccg cgcgccgtgg aaaggcactt gtgcttccag accgtgcgcc actttactct
    16681 ggtgcgcttt caccgcgtaa tcaatcacat cgcgagtttg ttctaacaga tcgagcatct
    16741 gatagctggc ctgcatactg taataaagct gcgctacgcc cgtggtcagc gatagctcta
    16801 ctgctgcggt ttctgccagc gcggcattat gcgcgccaat ggcggcggca accgctgagc
    16861 gatgcacacc ccacaaatca agatccagtc cggcaaacaa acctactgtg gcttccgtat
    16921 agtacggccc gtccataccc agtgcgggcg catccatcgc ataagggctt aaaaagccgt
    16981 tcgccgagac gcgttggcgg ttgagcatcc ctaacgctgc gacctgtaat tgtgaaccgg
    17041 catctaacaa atcggcctgg gactgcgctt tttcttcccg cagtttcgct tcggcgaggg
    17101 tgtgtgaacc acttagcgtc cgttggatca gcgcatccag ctgcgggtca ttgagttgtt
    17161 tccaccactg cgcctgcggc cagccggagc tggcaagatg aatatcatcg gccagtttga
    17221 tttgttccgg tttgagctgt tgatgaggcg cagaatcctt gcgtaccaag gcacagccgg
    17281 aaatcagcgt cgtgctgccg agaatgctgc acaacagcag acgtgaaagt tgacgattga
    17341 tcattgcgtg gctccttgtg cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc
    17401 tggcaagcgg gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg tgggcaggat
    17461 ggcctgcgag gcgggtgtct cttcaagcgt tatttgagga gaacggctga gtgcggttgc
    17521 cagaccggca gcgtattttt ccagggcgtc ggcaaactga cctgctctgt ccggctgtag
    17581 tgcgttatcc agcgcctgcg ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg
    17641 gccctgacga ataaccgttt gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag
    17701 ttgacgctcc agcgccacgc gttggcacat ctcttcacag gcattaaacg ccgcatgcag
    17761 accgatacga atttgcagat aagtgcgcag agccgtgact tcctgctggc gtgggatccg
    17821 cattacttta cttaacatac ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc
    17881 actttcaggc cagacaaagg tgtaaatcac cgcggacacc acggtaccaa tgatgatacc
    17941 cagggcgcga tcgcgaattt ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt
    18001 ggcgagcgcg aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt
    18061 ggcaatccat gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt
    18121 gtccagccag ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc
    18181 cccgccaaaa cgcagcacca tcttctggta cgacgaaccg acatttggat tagagacgat
    18241 cacgcaggtc agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta
    18301 acagatcaaa caggcgagca atgtttttac cgcgtagcgt atatagtctg gattggtaaa
    18361 agcatcggcg accattgatg gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat
    18421 ctggcccagt tgtaacaacg tctggcagat attctccagg ttacattccc gtgccgccac
    18481 cgcttcactt tcactgatcc gccagtcgct ttgccagcac tgaccttcag caacggcatg
    18541 ctgcagcttg ttgatttctg aggctaattt ttgccgaaat tcaataattg cctgagaatc
    18601 agcaaaagag gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac
    18661 gcagctttgc caccatgcgc tttgagttcg ccagttggca tcgtccgcga gacaaaagac
    18721 attcagtttt tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag
    18781 gctgtctgtc aggtgactaa tggcatcatc aagccgatca ttaagcgcct gatgcatttg
    18841 cgaaatggca cgactgggaa accacagcac gccgattaac gtcatcagca aggttggata
    18901 gaggccaaca acgatacacc acagcgttaa gcgcacgacc acttccggat agtcgagcat
    18961 ggcggggaag gtttgcccgt aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag
    19021 ccgatgggtg cgcatcaaaa acatgcggcc catcaggatc ggcccggcga tgatcaatcg
    19081 gatcaacggt tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac
    19141 cgtggcaacc acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc
    19201 gtaaaacagc actgccagcg ataacgccac aaaagggatc tcaaaagtca tcgagatcag
    19261 aatcaccagc aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg
    19321 ctcgcttaac tcttcatgaa agaacgccag cagcctgacc atcggtaatg gcagggagtt
    19381 gagcgcgctc attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt
    19441 tccgggtcag gtttatcgac catgattttg accggaaaac gctgggcaac gcggacccag
    19501 ttaatagagc gagaaacttt cggcaggccg cccagcacca ggccgccgtc atccggtagc
    19561 acgccgtagc caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg
    19621 cgaatcgttg cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg
    19681 atcacgtacc agtgacgagt gtcgattagg gtaaaaatag ggcgcatggc agaagcaaat
    19741 tgcccgacgg aggttttgag ggaaatgacc cggccatcaa acggcgcgcg aacggtcgcc
    19801 atttccagat gcagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc
    19861 agcgcatcga cgccgctgac ggcgctggcg gctgactgcg cctgtaacaa tacggcatta
    19921 agatcggctt ctgcggcgcg ctgcgcagtt cttgcacggt caacgtcttc cgctgagaca
    19981 aaaccctctt tcagtaatgg ctcggtacgg cgtaatgtat ctgtggcctg tttcgcggcg
    20041 gcacgggctt tttctaccgt ggcattaatc gagtcggcac caaactgttg cgcatcaacg
    20101 ctacgctggg tgagcataat ttgcttatcc agcgccgcga gggaggcttc agctttcgcc
    20161 agattggctt cgtacgggcg cgggtcaatg cggaacagca aatcgccctg tttgactgcc
    20221 tggttgtcgg tgaccgccag ttctacaatg cggccgctga cttccggcac cacatcaatg
    20281 gtatctgctg acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg ccagataacg
    20341 aaaacaaggg caaccagcgc caatgcaacc actaacagag cagggaattt actgcgagga
    20401 gcttttttcg gcgtactttc catgagcgcg tctcaaatag attagaaaaa tgccagccag
    20461 aacagcatgg cgtagagagc aaaaagggcg gtataaataa ttccgacaaa tgccagattg
    20521 atatttgccc gctgaataac tcgcctcgtg atgagcatca aaataaggct ggcaatggcg
    20581 cagaaaaacc agctgggata ataagcaccg atcaccggga ttgccggaga taatgagcat
    20641 cccgaaacca tgaagacggg aattatccag gctgtttttt tgagttgcat cgccatgcga
    20701 gagagaactg ttggcatatt aaatacatcc tgtctgctgc gaacaatatc tgtagtgaat
    20761 atcaatatcc attgaaggcg tcagtatggc tattttctcg atgaataaaa atagctttat
    20821 tcagtcaccg cattcataac ttatatattt tatttattaa ttggctgtat atatttttaa
    20881 aataaatgct tttgggggct tcagcgcccc caaaagcgag tgacattaat ttggggttac
    20941 gatattcacc cacaaatcga aattatccag acaggcgata atttcttcca gtttcgcgcc
    21001 attgccaata attttggctt tcttcgcttt taccagatcc gccattttgg cttgcccggt
    21061 cagcacggcg tgcagatctt cacggctgat gtagaaagag gcgtcagctt gggattgcag
    21121 tgttttacgg tagttaagca cgctatcgtt cagcgtcagg ttgaggttat cgccattgct
    21181 catattgaag ttcaggctga tatttttacc cgcggctttc gcgctatcga ggcgaacgga
    21241 cataaagtcg aacagcattt cgaccgacat cccgcgaatg gtgtccgggg aaccgatggt
    21301 gccgtggctg aacttatgca ccccttcgcg cagctctttc gcgccggtca ggtagaaacc
    21361 gcgccaggtg gcggactcgg cctgatagcc cagctgttca aagttattcg cttgcagatt
    21421 cttcgcgacc tggtcacccg ggttggcggc aatcacctgt ttcagcagtt ctgccgacca
    21481 gcggtaatca ccttgcttgt tcgcttcttg cgccaggttg atgacacggg cagaaccgcc
    21541 cagcgcctgc acgtaacgtt tacccatctc cacctgacca tacggatgca ggttagccgg
    21601 gttaccgtcg taatagccaa gatagaagtt atacaccgcg cgggcgttgt ggctgacaga
    21661 accgtaatag ccacggctgg cccagttatt ggcaagtgca ggcggcagtt taatcatgtc
    21721 gccgatttca ttcatggtgt agccctggtt cgccaggtgc agggtctggt cgtgaatgta
    21781 cttgatagta tcgcgatatt taccaatata atcattgata tgcttattgc cccagaccgg
    21841 ccaggtgtgc ggcataaaca ggacttccgc gtcgttaccc cacatatcca gcgtttcgtt
    21901 cagatactcg gtccacttgc tggtgtcgcg ggttttcgcg ccgcgcagag tgtagaagtt
    21961 gtgcagggta tgcgtggcgt tctcggcggt acacagcgct ttcagggccg gaatatagaa
    22021 gtgcatttcg gctggtgctt cgctacctgg ggtcatcagg aagtcaaact ccaggccgtc
    22081 gataatcatc ttctcaccgg ttttggtgat ggttttgttc ggtgcgatga tgctcgggta
    22141 gcccgatgcc agcgttacgc caagaccatt gccgatgtta ccctgagggt tatgcgccag
    22201 cagcaggccg taggagtaaa gtgcacggcg gctcataata ttacccgcca gtacgttttc
    22261 gctgatggct tcgtccataa agccagcagg ggcgataacc tgcactttac cggatttcac
    22321 atcagcttca gagataatgc ctttcacgcc gccatagtgg tcggtatggc tgtgggtata
    22381 aataaccgca acaatcggtt tttgcgggcg attttggaag taaaggtcaa gggcggcttt
    22441 tgcgctcggt ggcgtcacca gcgggtcgat gacaataatc ccggtatcac cttcgataaa
    22501 ggtgatgttg gagatatcct gaccgcgaac ctggtacatt ctgtcggtga ctttaaacag
    22561 gccggaaata ccgtttaact gcgactgacg ccacaggcta gggttaacgg tttcgggcgc
    22621 gggcgcattg atatcaaatt tataatcttc cccgcgataa tatggtttgc cattagcatc
    22681 gcgcagaata ccttcatcca gcagcggagc gataaatcca cgctgagcgt cgtcaaaatc
    22741 ctgacgatcg ctaaacggta atgatttggc gtaattctga ttaacctgtt gggtatattg
    22801 agtggcggct ttcgcttcct ctttcgcgtt agcggtattt accatcatgc cgctggcggc
    22861 agttaacgca ataacaattc tgctcaaacg gaataaccga gagttattca tgtaaatctc
    22921 catttatcat ttatgtcgta aatatgtaat ccatgcaaaa tacgtcattt aaagctgggg
    22981 ttatcataat tgggaatttg cgactttaat aagtggaagt gtgagctgaa cgatccattt
    23041 tattaggcat ttgtgctgaa gagggaaaat tacgcgaatt tattatctct gtaaataaca
    23101 tgaggtgttt tatatattta ttatatatat tgtacttcgg caaatactga gcacaattta
    23161 tcaatgcaac cggtgtttct ttttaaaaaa cgtaccggag caatgggcgg tcatgtcgct
    23221 ggcaccgcag gattctgcgt tgctgtgcca gccttttatt catggtcaat gagtgaggtg
    23281 attgataaag acaccaggtc gtcagagcgc actgattatc cgcattatgc cagtgaagtt
    23341 gcactggctt ttggttcacg agtggttggc ctcgaactgg caaaaatgat tgtggatgcg
    23401 tggctgggcg cacagtacga aggcggtcgt catcaacaac gcgtggaggc gattacggca
    23461 atagagcagc ggagaaattg agattcatcc actacttgca tggatgagta atgactaatg
    23521 tggatagagt ttctttttga ggttggctaa tgaaacgctt tccgctattt cttttattca
    23581 ccctgctcac gctgtccacc gttccggccc aggctgatat tatcgacgat actattggta
    23641 atattcagca ggcgattaat gacgcttata accctgaccg tgggcgagac tatgaagatt
    23701 cgcgcgacga cggctggcag cgtgaagtga gcgacgatcg gcgcagacaa tatgacgacc
    23761 gccgccgcca gtttgaagat cgccgcaggc agctggacga tcgccagcgc cagcttgatc
    23821 aggagcgtcg gcaactggag gatgaagagc ggagaatgga agatgagtat gggcgatgag
    23881 gttgggtatg gagcaggcat cgcccctcac cccaatgggg agagggagaa aacgagcgca
    23941 atattcaata ttgagcacaa tcggtcccct cgcccctctg gggagagggt tagggtgagg
    24001 gggccagcgc gcacattccc tctcatcacg ccaccccaat ctccatccca tcaaacccca
    24061 cctcaaaccc tgacggtagt gcgttttcca tcagccacgc atcaaactgg tggctgatat
    24121 gggtcagaat cacccgtggc gagcggataa cctgattcag cgcaagcacg gtatttaaat
    24181 cacagtgatt acgcggtgca tccgcgcgcg gcgggtgact gcaatccatc accattacct
    24241 gcggctgatt attgcgtaaa aatttcagcg ttttttccgg caagccagcg gtgtcagaca
    24301 gccacgccac ccggctgtgc gccgtttcca gtaaatagcc gaaagtgagt tttgagtggt
    24361 taagcggcag cggcgtgacc tgcaacccct gcaaatcaaa caccacaaac ggttccaccg
    24421 tgtggctgaa atcaagcagc ccgggatgtt taaacagatc gtcgcagccc tgttcatccg
    24481 gcgggccgta gatcgggatc ggatcgccca cgccccagcg cagcggaaac agcccctgga
    24541 cgtgatccat atgataatgc gtcagcaaaa actgctggaa cgatccggga gaccagcgat
    24601 cggtgagatc gtgcagcccg gcgtcgatca gggtgatcgc ctcgttaaac ttcactacgc
    24661 cgctgcacgg ttggcggcga t
//
LOCUS       DAJTYZ010000056        24664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000056 DAJTYZ010000000
VERSION     DAJTYZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24664
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.056"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..121)
                     /locus_tag="OO596_002137"
     CDS             complement(<1..121)
                     /locus_tag="OO596_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCR6717991.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLA"
     gene            complement(179..412)
                     /locus_tag="OO596_002138"
     CDS             complement(179..412)
                     /locus_tag="OO596_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR6717992.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            801..1346
                     /locus_tag="OO596_002139"
     CDS             801..1346
                     /locus_tag="OO596_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR6717993.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1321..3246
                     /locus_tag="OO596_002140"
     CDS             1321..3246
                     /locus_tag="OO596_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6717994.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3243..3449
                     /locus_tag="OO596_002141"
     CDS             3243..3449
                     /locus_tag="OO596_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCR6717995.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3446..5047
                     /locus_tag="OO596_002142"
     CDS             3446..5047
                     /locus_tag="OO596_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6717996.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5028..6347
                     /locus_tag="OO596_002143"
     CDS             5028..6347
                     /locus_tag="OO596_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6717997.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6357..6689
                     /locus_tag="OO596_002144"
     CDS             6357..6689
                     /locus_tag="OO596_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6717998.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            6745..7770
                     /locus_tag="OO596_002145"
     CDS             6745..7770
                     /locus_tag="OO596_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6717999.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7812..8210
                     /locus_tag="OO596_002146"
     CDS             7812..8210
                     /locus_tag="OO596_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6718000.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8222..8575
                     /locus_tag="OO596_002147"
     CDS             8222..8575
                     /locus_tag="OO596_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR6718001.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8587..9165
                     /locus_tag="OO596_002148"
     CDS             8587..9165
                     /locus_tag="OO596_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6718002.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9162..9557
                     /locus_tag="OO596_002149"
     CDS             9162..9557
                     /locus_tag="OO596_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6718003.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            9565..10305
                     /locus_tag="OO596_002150"
     CDS             9565..10305
                     /locus_tag="OO596_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6718004.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10321..10743
                     /locus_tag="OO596_002151"
     CDS             10321..10743
                     /locus_tag="OO596_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6718005.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            10725..11159
                     /locus_tag="OO596_002152"
     CDS             10725..11159
                     /locus_tag="OO596_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6718006.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11152..13713
                     /locus_tag="OO596_002153"
     CDS             11152..13713
                     /locus_tag="OO596_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6718007.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            13710..14039
                     /locus_tag="OO596_002154"
     CDS             13710..14039
                     /locus_tag="OO596_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6718008.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14039..14737
                     /locus_tag="OO596_002155"
     CDS             14039..14737
                     /locus_tag="OO596_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6718009.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14743..15486
                     /locus_tag="OO596_002156"
     CDS             14743..15486
                     /locus_tag="OO596_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6718010.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15423..16055
                     /locus_tag="OO596_002157"
     CDS             15423..16055
                     /locus_tag="OO596_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6718011.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16116..19613
                     /locus_tag="OO596_002158"
     CDS             16116..19613
                     /locus_tag="OO596_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6718012.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            19684..20200
                     /locus_tag="OO596_002159"
                     /pseudo
     CDS             19684..20200
                     /locus_tag="OO596_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20265..21669
                     /locus_tag="OO596_002160"
                     /pseudo
     CDS             20265..21669
                     /locus_tag="OO596_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            21618..21770
                     /locus_tag="OO596_002161"
                     /pseudo
     CDS             21618..21770
                     /locus_tag="OO596_002161"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            21747..21926
                     /locus_tag="OO596_002162"
                     /pseudo
     CDS             21747..21926
                     /locus_tag="OO596_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            22641..23348
                     /locus_tag="OO596_002163"
                     /pseudo
     CDS             22641..23348
                     /locus_tag="OO596_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23348..23929
                     /locus_tag="OO596_002164"
     CDS             23348..23929
                     /locus_tag="OO596_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6718013.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         5911 a         6104 c         7460 g         5189 t
ORIGIN      
        1 tggctaaata gtaaatgtaa tgtgagacat tgtgacgttt tagttcagaa taaaaccagt
       61 gatagtttaa attatttcgc actttatcga atatttgttt aaaaatggca acctgagcca
      121 ttgtagtacc ttccatgtga tatgaggggg cgtagtctgc acgattatct aaattgcttc
      181 aatctggtct aacctgtttt ctgagcaatt cagtaatgtc actcttttct ttgtttgctt
      241 caggcgaaac tcttttttct gagcacagtc tccggcggca ggcttcaatg acccaggctg
      301 agaaattccc ggaccctttt tgaacaagag cgatgttaat ttgttcaatc atttggttag
      361 gaaagcggat gttgcgggtt gttgttctgc gggttctgtt cttcgttgac atgaggttgc
      421 cctgtattca gtgtcgctga tttgtattgt ctgaagttgt ttttacgtta agttgatgca
      481 gatcaattaa tacgatacct gcgtcataat tgattatttg acgtggtttg atggcgtaga
      541 tgcacgttgt gacatgtaga tgataattat tatcattttg cgggtccttt ccggcgatcc
      601 gacaggttac ggggcggaag gcgcgcgggt tttcgctatt tatgacaatt ttccggttta
      661 aggcgtttcc gttcttcttc gccgtaacct aatgttttta tttaaaacac cccctgaaaa
      721 gaaaggaaac gacaggtgct gaaaacgggc tttttggcct ctgtcgtttc ctttctctgt
      781 ttttgtccgt ggaatgaaca atggaagtca acaaaaagca gctggctgac attttcggtg
      841 cgagtatccg taccattcag aactggcagg aacagggaat gcccgttctg cgaggcggtg
      901 gcaagggtaa tgaggtgctt tatgactctg ccgccgttat aagatggtat gccgaaaggg
      961 atgctgaaat tgagaacgaa aagctgcgcc gggaagttga agaactgcgg caggccagcg
     1021 agacagatct ccagccaggg actattgagt acgaacgcca tcgacttacg cgtgcgcagg
     1081 ccgacgcaca ggagctgaaa aatgccagag actccgctga agtggtggaa accgcattct
     1141 gtactttcgt gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggatccccc
     1201 tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg
     1261 atatcatcaa agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga
     1321 gtgaatatat cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc
     1381 tcactgttca ggccggagcc acagaccgcc gttgaatggg cggatgctaa ttactatctc
     1441 ccgaaagaat ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc
     1501 atgaatgcga tgggcagcga ctacatccgt gaggtgaatg tggtgaagtc tgcccgtgtc
     1561 ggttattcca aaatgctgct gggtgtttat gcctacttta tagagcataa gcagcgcaac
     1621 acacttatct ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag
     1681 ccgaccatcc gcgatattcc gtcgctgctg gcgctggctc cgtggtatgg caaaaagcac
     1741 cgggataaca cgctcactat gaagcgtttt tccaatggtc gtggcttctg gtgcctgggc
     1801 ggtaaagcgg caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta tgatgaactt
     1861 gctgcctttg atgaggatat tgaacaggaa ggctctccga cgttccttgg cgacaaacgt
     1921 attgaaggct cggtctggcc aaagtccatc cgtggctcca cgcccaaagt gagaggcacc
     1981 tgccagattg agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc
     2041 ccgcactgcg gggaggagca gtatcttaaa tttggcgaca aagagacgcc gtttggcctc
     2101 aaatggacgc cggatgaccc ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc
     2161 atccgccagc aggagctgga ctttactgat gcccgttata tctgcgaaaa gaccgggatc
     2221 tggacccgtg atggcattct ctggttttcg tcatccggtg aagagattga gccacctgac
     2281 agtgtgacct ttcacatctg gacagcgtac agcccgttca ccacctgggt gcagattgtc
     2341 aaagactgga tgaaaacgaa aggggatacg ggaaaacgta aaaccttcgt aaacaccacg
     2401 ctcggtgaga cgtgggaggc gaaaattggc gaacgtccgg atgctgaagt gatggcagag
     2461 cggaaagagc attattcagc gtccgttcct gaccgtgtgg cttacctgac cgccggtatc
     2521 gactcccagc gggatcgcta cgaaatgcgc gtatggggat gggggccggg tgaggaaagc
     2581 tggctgattg atcggcagat tattatgggc cgccacgacg atgaacagac gctgctgcgt
     2641 gtggatgagg ccatcaataa aacctatacc cgccggaatg gtgcagaaat gtcgatatcc
     2701 cgtatctgct gggatattgg cgggattgac ccgaccattg tgtatgaacg ctcgaaaaag
     2761 catgggctgt tccgggtgat ccccattaaa ggggcatccg tctacggtaa gccggtggcc
     2821 agcatgcctc gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg tacggatacc
     2881 gcgaaagagc agatttataa ccgcttcaca ctgacgccgg aaggggatga accgcttccc
     2941 ggtgccgttc acttcccgaa taacccggat atttttgatc tgaccgaagc gcagcagctg
     3001 actgctgaag agcaggtcga aaaatgggtg gatggcagga aaaaaatact gtgggacagc
     3061 aaaaagcgac gcaatgaggc gctcgactgc ttcgtttatg cgctggcggc gctgcgcatc
     3121 agtatttccc gctggcagct ggatctcagt gcactgctgg cgagcctgca ggaagaggat
     3181 ggtgcagcaa ccaacaagaa aacactggca gattacgccc gtgccttatc cggagaggat
     3241 gaatgacgcg acaggaagaa cttgccgctg cccgtgcggc actgcatgac ctgatgacag
     3301 gtaaacgggt ggcaacggta cagaaagacg gacggcgagt ggagtttacg gccacttccg
     3361 tgtctgacct gaaaaaatac attgcggagc tggaggtgca gaccggcatg acacagcgac
     3421 gcaggggacc tgcaggattt tatgtatgaa aacgcccacc attcccaccc ttctgggacc
     3481 ggacggcatg acatcgctgc gtgaatatgc cggttatcac ggcggtggca gcggatttgg
     3541 tgggcagttg cgggcgtgga acccaccggg tgaaagtgtg gatgcagccc tgctgcccaa
     3601 ctttacccgt ggcaatgccc gcgcagacga tctggtacgc aataacggct atgctgccaa
     3661 cgccatccag ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc
     3721 aagctggcgc tatctgggca tcggggagga agacgcccgt gccttttccc gcgaggttga
     3781 agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg
     3841 cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt
     3901 cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt
     3961 cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg
     4021 tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg acggctatcc
     4081 tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcctc
     4141 gttcattcac gtttttgaac ccgtggagga cgggcagacc cgcggtgcaa atgtgtttta
     4201 cagcgtaatg gagcagatga agatgctcga cacgctgcag aacacgcagc tgcagagcgc
     4261 cattgtgaag gcgatgtatg ccgccaccat tgaaagtgag ctggatacgc agtcagcgat
     4321 ggattttatt ctgggcgcga acagtaagga gcagcgggaa aggctgacgg gctggattgg
     4381 tgaaattgcc gcgtattacg ccgcagcacc ggtccgtctg ggaggcgcaa aagtgccgca
     4441 cctgatgccg ggggactcac tgaacctgca gacggctcag gacacggata acggctactc
     4501 cgtgtttgag cagtcactgt tgcggtatat cgctgccggg ctgggtgtct cgtatgagca
     4561 actttcccgg aattacgccc agatgagcta ctccacggca cgggccagtg cgaacgagtc
     4621 gtgggcgtac tttatggggc ggcgaaaatt cgtcgcatcc cgtcaggcga gccagatgtt
     4681 tctgtgctgg ctggaagagg ccatcgttcg ccgcgtggtg acgttacctt caaaagcgcg
     4741 cttcagcttt caggaagccc gcagtgcctg ggggaactgc gactggatag gctccggtcg
     4801 tatggccatc gatggtctga aagaagttca ggaagcggtg atgctgatag aagccggact
     4861 gagcacctac gagaaagagt gcgcgaaacg cggtgacgac tatcaggaaa tttttgccca
     4921 gcaggtccgt gaaacgatgg agcgccgcgc agctggtctt aaaccgcccg cctgggcggc
     4981 tgcggcattt gaatccgggc tgcgacaatc aacagaggag gagaagagtg acagcagagc
     5041 tgcgtaatct cccgcatatt gccagcatgg cttttaatga gccgctgatg cttgaacccg
     5101 cctatgcgcg ggttttcttt tgtgcgcttg caggccagct tgggatcagc tgcctgacgg
     5161 atgcagtatc cggcgacagc ctgactgccg gagaggcacc cgcggcgctg gcgttatccg
     5221 gtgatgatga cggaccacga caggcccgca gttatcaggt aatgaacggc atcgccgtgc
     5281 tgccggtgtc cggcacgctg gtcagccgga cgcgggcgct gcagccgtac tcggggatga
     5341 ccggttacaa cggcattatc gcccgtctgc aacaggctgc cagcgacccg atggtggacg
     5401 gcattctgct cgatatggac acaccgggcg gaatggtggc gggagcattt gactgcgctg
     5461 acatcatcgc ccgtgtgcgt gacataaagc cggtatgggc gctggccaat gacatgaact
     5521 gcagcgcagg tcagctgctt gccagtgccg cctcccggcg tctggtcacg cagaccgccc
     5581 ggacaggctc catcggcgtc atgatggctc acagtaatta cggcgctgca ctggagaaac
     5641 agggcgtgga aatcacgctg atttacagcg gcagccataa ggtggatggc aacccctaca
     5701 gccatctacc ggatgatgtc cgggaaacat tgcagtcccg gatggatgca acccgccgga
     5761 tgtttgcaca gaaggtgtcg gcatataccg gcctgtccgt gcaggctgtg ctggataccg
     5821 aggctgcagt gtacagcggt caggaggcca ttgatgccgg actggctgat gaacttgtta
     5881 acagtaccga tgcgatcacc gtcatgcgtg atgcactgga tgcacgtaaa tcccgtctct
     5941 caggagggcg aatgaccaaa gagactcaat caacaactgt ttcagccact gcttcgcagg
     6001 ctgacgttac tggcgtggtg caagcgacgg agggcgagaa cgccagcgcg gcgcagccgg
     6061 acgtgaacgc gcagatcacc gcagcggttg cggcagaaaa cagtcgcatt atggggatcc
     6121 tcaactgtga ggaggctcac ggacgcgaag aacaggcacg cgtgctggcc gaaacccccg
     6181 gtatgaccgt ggaaacggcc cgccgtattc tggccgcagc accacagagt gcacagacgc
     6241 gcagtgacac tgcgctggat cgtctgatgc agggggcacc ggcaccgctg gctgcaggta
     6301 acccggcatc tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg atgtttatga
     6361 cgagcaaaga aacctttacc cattaccagc cgctgggcaa tagtgacccg gctcataccg
     6421 caaccgcgcc cggcggattg agtgcgaaag cgcctgcaat gaccccgctg atgctggaca
     6481 cctccacccg taagctggtt gcgtgggatg gcaccaccga cggtgctgcc gttggcattc
     6541 ttgcggttgc tgctgaccag accagcacca cactgacgtt ctacaagtcc ggcacgttcc
     6601 gttatgagga tgtgctctgg ccggaggctg ccagcgacga gacgaaaaaa cggaccgtgt
     6661 ttgccggaac ggcaatcagc atcgtttaac tttacccttc atcactaaag gccgcctgtg
     6721 tggctttttt tacgggattt ttttatgtcg atgtacacaa ccgcccagct gctggcggca
     6781 aatgagcaga aatttaagtt tgatccgctg tttctgcgtc tctttttccg tgagagctat
     6841 cccttcacta cggagaaagt ctatctctca caaattccgg gactggtaaa catggcgctg
     6901 tacgtttcgc cgattgtttc cggtgaggtt atccgttccc gtggcggctc cacctctgaa
     6961 tttacgccgg gatatgtcaa acccaagcat gaggtgaatc cgcagatgac cctgcgtcgc
     7021 ctgccggatg aagatccgca gaatctggcg gacccggctt accgccgccg tcgcatcatc
     7081 atgcagaaca tgcgtgacga agagctggcc attgctcagg tcgaagagat gcaggcagtt
     7141 tctgccgtgc tcaagggcaa atacaccatg accggtgaag ccttcgatcc ggttgaggtg
     7201 gatatgggcc gcagtgcggc gaacaacatc acacagtccg gcgtcacgga gtggagcaag
     7261 cgtgacaagt ccacgtatga cccgaccgac gatatcgaag cctacgcgct gaacgccagc
     7321 ggtgtggtga atatcatcgt gtttgacccg aaaggctggg cgctgttccg ttccttcaaa
     7381 gccgtcaagg agaagctgga tacccgtcgc ggctctcatt ccgagctgga gacagcggta
     7441 aaagacctgg gcaaagcggt gtcctataag ggaatgtatg gcgatgtggc catcgtcgtg
     7501 tattccggac agtacgtgga aaacggcgtc aaaaagaact tcctgccgga caacacgatg
     7561 gtgctgggga acactcaggc tcgcggtctg cgcacctatg gctgcattca ggatgcggac
     7621 gcacagcgcg aaggcattaa cgcttctgcc cgttacccga aaaactgggt gaccaccggc
     7681 gatccggcgc gtgagttcac catgattcag tcagcaccgc tgatgctgct ggctgatcct
     7741 gatgagttcg tgtccgttca actggcgtaa tcatggccct tcggggccat tttctctctg
     7801 tggaggagtt catgacgaaa gatgaactga ttgcccgtct tcaggagctg ggtgagcaat
     7861 tgaaccgcga tgtcagtttg acgggaacga aagaagaact ggcgcttcgt gtggcagagc
     7921 tggaagaaga gcttgatgac acggatgaaa ctgccggtca ggacacccct ctcagccggg
     7981 aaaatgtgct gaccgggcat gaaaatgagg tggtatcagc gcagccggat accgtgattc
     8041 aggatacggc tgaactggtc acggtcgtgg cactggtgac gctgcatact gatgcacttc
     8101 acgccacgcg ggataaacct gtggcatttg tgctgccggg aacggcgttt cgtgtctctg
     8161 ccggtgtggc agccgaaatg acagagcgcg gcctggccag aatgcaataa cgggaggcgc
     8221 tgtggctgat ttcgataacc tgttcgatgc tgccattgcc cgcgccgatg aaacgatacg
     8281 cgggtacatg ggaacgtcag ccaccatgac atccggtgag cagtcaggtg cggtgatacg
     8341 tggtgttttt gatgaccctg aaaatatcag ctatgccgga cagggcgtgc gcgttgaagg
     8401 ctccagcccg tccctgtttg tccggactga tgaggtgcgg cagctgcggc gtggagacac
     8461 gctgaccatc ggtgaggaaa atttctgggt agatcgggtt tcgccggatg atggcggaag
     8521 ttgtcatctc tggcttggac ggggcgtacc gcctgccgtt aaccgtcgcc gctgaaaggg
     8581 ggatgtatgg ccataaaagg tcttgagcag gccgttgaaa acctcagccg tatcagcaaa
     8641 acggcggtgc ccggtgccgc cgcaatggcc attaaccgcg ttgcttcatc cgcgatatcg
     8701 cagtctgcgt cacaggttgc ccgtgagaca aaggtacgcc ggaaactggt aaaggaaagg
     8761 gccaggctga aaagggccac ggtcaaaaat ccgcaggcca gaatcaaagt taaccggggg
     8821 gatttgcccg taatcaagct gggtaatgcg cgggttgtcc tgtcccgccg caggcgtcat
     8881 aaaaaggggc agcgttcatc cctgaaaggt ggcggcagcg tgcttgtggt gggtaaccgt
     8941 cgtattcccg gcgcgtttat tcagcaactg aaaaacggcc ggtggcatgt tatgcagcgt
     9001 gtggccggga aaaaccgtta ccccattgat gtggtgaaaa tcccgatggc ggtgccgctg
     9061 accacggcgt ttaaacagaa tattgaacgg atacggcgtg aacgtcttcc gaaagagctg
     9121 ggctatgcgc tgcagcatca actgagaatg gtaataaagc gatgaaacat actgaactcc
     9181 gtgcagccgt actggatgca ctggagaagc atgacaccgg ggcgacgctt tttgatggtc
     9241 gccccgctgt ttttgatgag gaagattttc cggcaattgc cgtttatctc accggcgctg
     9301 aatacacggg cgaagagctg gacagcgata cctggcaggc ggagctgcat attgaagttt
     9361 tcctgcctgc tcaggtgccg gattcagagc tggattcgtg gatggagtcc cggatttatc
     9421 ctgtgatgag cgatatcccg gcactgtcag atttgatcac cagtatggtg gccagcggct
     9481 atgactaccg gcgcgacgat gatgcgggcc tgtggagttc agccgatctg acttatgtca
     9541 ttatctatga aatgtgagga cgctatgcct gtaccaaatc ctacaatgcc ggtgaaaggt
     9601 gccgggacca ccctgtgggt ttataagggg agcggtgacc cttatgcgaa cccgctttca
     9661 gacgttgact ggtcgcgtct ggcaaaagtt aaagacctga cgcccggcga actgaccgct
     9721 gagtcctatg acgacagcta tctcgatgat gaagatgcag actggactgc gaccgggcag
     9781 gggcagaaat ctgccggaga taccagcttc acgctggcgt ggatgcccgg agagcagggg
     9841 cagcaggcgc tgctggcgtg gtttaatgaa ggtgataccc gtgcctataa aatccgcttc
     9901 ccgaacggca cggtcgatgt gttccgtggc tgggtcagca gtatcggtaa ggcggtgacg
     9961 gcgaaggagg tgattacccg cacggtgaag gtcaccaatg tgggacgtcc gtcgatggca
    10021 gaagatcgca gcacggtgac ggcgacgacc ggcatgacgg tgacacccgc cagtgcttcc
    10081 gtagtgaaag ggcagagcac cacgctgacc gtggcattcc agccggatgg cgcaaccgac
    10141 aagagcttcc gtgcggtgtc tgcggataaa acaaaagcca ccgtgtcggt cagtggtatg
    10201 accatcaccg tgaaaggtgt tgctgcaggc aaggtcaaca ttccggtcgt atctggtaat
    10261 ggtgagtttg ctgcggttgc agaaatcaac gtcaccgcca gttaatccgg agagtcagcg
    10321 atgttcctga aaaccgaatc atttgaacat aacggtgtga ccgtcacgct ttctgaactg
    10381 tcagccctgc agcgtattga gcatctcgac ctgatgaaac ggcaggcaga acaggcggag
    10441 tcagacagca accggaagtt tactgtggaa gacgccatca gaaccggcgc ttttgtggtt
    10501 gcgatgtccc tgtggcataa ccatccgaag aagacgcaga tgccgtccat gaatgaagcc
    10561 gttaaacaga ttgagcagga agtgcttacc acctggccca cagaggcaat ttctcatgct
    10621 gaaaacgtgg tgtaccggct gtccggtatg tatgagtttg tggtgaatga tgctcctgaa
    10681 caggcagagg acgccgggcc tgcagagcct gtttctgcgg gaaagtgttc gacggtgagc
    10741 tgagttttgc cctgaaactg gcgcgtgaga tggggcgacc cgactggcgc gccatgcttg
    10801 ccgggatgtc atccacggag tatgccgact ggcaccgctt ttacagtacc cattattttc
    10861 atgatgttct gctggatatg cacttttccg ggctgacgta caccgtgctc agcctgtttt
    10921 tcagcgatcc ggagatgcat ccgctggatt tcagtctgct gaaccggcgc gaggctgacg
    10981 aagagcctga agatgatgtg ctgatgcaga aagcggcagg gcttgccgga ggtgtccgct
    11041 ttggcccgga cgggaatgaa gttatccccg cttccccgga tgtggcggac atgacggagg
    11101 atgacgtaat gctgatgaca gtatcagaag ggatcgcagg aggagtccgg tatggctgaa
    11161 ccggtaggcg atctggtcgt tgatttgagt ctggatgcgg ccagatttga cgagcagatg
    11221 gccagagtca ggcgtcattt ttccggtacg gaaagtgatg cgaaaaaaac agcggcagtc
    11281 gttgaacagt cgctgagccg acaggcgctg gctgcacaga aagcggggat ttccgtcggg
    11341 cagtataaag ccgccatgcg tatgctgcct gcacagttca ccgacgtggc cacgcagctt
    11401 gcaggggggc agaatccctg gctgatcctg ctgcaacagg gtggtcaggt taaggactcc
    11461 ttcggcggga tgatccccat gttcaggggg cttgccggcg cgatcaccct gccgatggtg
    11521 ggggccacct cgctggcggt ggcgaccggt gcgctggcgt atgcctggta tcagggcaac
    11581 tcaaccctgt ccgatttcaa caaaacgctg gtcctttccg gtaatcaggc gggactgacg
    11641 gcagatcgta tgctggtcct gtccagagcc gggcaggcgg cagggctgac gtttaaccag
    11701 accagcgagt cactcagcgc actggttaag gcgggggtaa gcggtgaggc tcagattgcg
    11761 tccatcagcc agagtgtggc gcgtttctcc tatgcatccg gcgtggaggt ggacaaggtc
    11821 gctgaagcct tcgggaagct gaccacagac ccgacgtcgg ggctgacggc gatggcacgc
    11881 cagttccata acgtgacggc ggagcagatt gcgtatgttg ctcagttgca gcgttccggc
    11941 gatgaagccg gggcattgca ggcggcgaac gaggccgcaa cgaaagggtt tgatgaccag
    12001 acccgccgcc tgaaagagaa catgggcacg ctggagacct gggcagacag gactgcacgt
    12061 gcattcaaat ccatgtggga tgcggtgctg gatattggtc gtcctgatac cgcgcaggag
    12121 atgctgatta aggcagaggc tgcgtttaag aaagcagacg acatctggaa tctgcgcaag
    12181 gatgattatt tcgttaacga tgaagcacgg gcgcgttact gggatgatcg tgaaaaaaaa
    12241 cgcctggagc gggatgcagc ccaaaagagg gtagatcaac agcgtcaaca ggacaaaaat
    12301 gcgcagcagc agagcgatac cgaagcgtca cggctgaaat ataccgaaga ggcgcagaag
    12361 gcttacgaac ggctgcagac gccgctggag aaatataccg cccgtcagga agaactgaac
    12421 aaggcactga aagacggaaa aatcctgcag gcagattaca acacgctgat ggcggcggcg
    12481 aaaaaggatt atgaagcgac gctgaaaaag ccgaaacagt ccggcgtgaa ggtgtatgcg
    12541 ggcgatcgtc aggaagacag tgctcatgcg gccctgctga cgcttcaggc agaactccgg
    12601 acgctggaga agcatgccgg agcgaatgag aaaatcagcc agcagcgccg ggatttgtgg
    12661 aaggcagaaa gtcagttcgc ggtactggag gaggcggcac aacgtcgcca gctgtccgca
    12721 caggagaaat ccctgctggc gcataaagac gagacgctgg agtacaaacg ccagctggct
    12781 gcacttggtg acaaggttac gtatcaggag cacctgaatg cgctggcgca gcaggcggat
    12841 aaattcgcac agcagcaacg ggcaaaacgg gcagccattg atgcgaaaaa ccgggggctg
    12901 actgaccggc aggcagcgcg ggaagccacg gaacagcgcc tgaaggaaca gtatggcgat
    12961 aatcctctgg cgctgaataa cgtcatgtca gagcagaaaa agacctgggc ggctgaagac
    13021 cagcttcgcg ggagctggat ggcaggcctg aagtccggct ggagtgagtg ggaagagagc
    13081 gccacggaca gtatgtcaca ggttaaaagt gcagccacgc agacctttga tggtattgca
    13141 cagaatatgg cggcgatgct gaccggcagt gaacagaact ggcgcagctt cacccgctcc
    13201 gtgctgtcca tgatgacaga aattctgctt aagcaggcaa tggtggggat tgtcgggagt
    13261 atcggcagcg ccattggcgg ggctgttggt ggcggcgcat ccgcgtcagg tggtacagcc
    13321 attcaggccg ctgcggtgaa attccatttt gcaaccgggg gatttacggg aaccggcggc
    13381 aaatatgagc cagcggggat tgttcaccgt ggtgaatttg tcttcacgaa ggaggcaacc
    13441 agccggattg gcgtggggaa tctctaccgg ctgatgcgcg gctatgcgga aggtggttat
    13501 gtgggcggtg ccggaagtcc ggcgcagatg cggcgggctg aaggcattaa ttttaatcag
    13561 aacaatcacg tggtgattca gaacgacggt acgaatggtc tgccaggtcc acagatgatg
    13621 aaggccgtgt atgacatggc ccgcaagggt gcccgtgatg aaattcagac acagatgcgt
    13681 gatggtggcc tgttctccgg aggtggacga tgaaaacctt ccgctggaaa gtgaaacccg
    13741 gtatggatgt ggcttcggcc ccttctgtaa gaaaggtgcg ctttggtgat ggctattccc
    13801 agcgagcgcc tgccgggctg aatgccaacc tgaaaacgta cagcgtgacg ctttctgtcc
    13861 cccgtgagga ggccatggta ctggagtcgt ttctggaaga gcacgggggc tggaaagcct
    13921 ttctgtggac gccgccttat gagtggcggc agataaaggt gacctgcgca aaatggtcgt
    13981 cgcgggtcag tatgctgcgt gttgagttca gcgcagagtt tgaacaggtg gtgaactgat
    14041 gcaggatatc cggcaggaaa cactgaatga atgcacccgt gcggagcagt cggccagcgt
    14101 ggtgctctgg gaaatcgatc tgacagaggt cggtggagaa cgttattttt tctgtaatga
    14161 gcagaacgaa aaaggtgagc cggtcacctg gcaggggcga cagtatcagc cgtatcccat
    14221 tcaggggagt ggttttgaac tgaatggcaa aggcaccagt acgcgcccca cgctgatggt
    14281 ttctaacctg tacggtatgg tcaccgggat ggcggaagat ctacagagtc tggtcggcgg
    14341 aacggtggtc cggcgtaagg tttacgcccg ttttctggat gcggtgaact tcgtcaacgg
    14401 aaacagtgac gccgatccgg agcaggaggt gatcagccgc tggcgcattg agcagtgcag
    14461 cgaactgagc gcggtgagtg cctcttttgt actgtccacg ccgacggaaa cagatggcgc
    14521 tgtttttccg ggacgtatca tgctggccaa cacctgcacc tggacctatc gcggcgatga
    14581 gtgcggttat cacggtccgg cggtcgcgga tgaatatgac cagccaacgt ccgacatcac
    14641 gaaggataaa tgcagcaaat gcctgagtgg ctgtaagttt cgcaataacg tcggcaactt
    14701 tggcggcttc ctttccatta acaaactttc gcagtaaatc ccatgacaga gacagaatca
    14761 gcgattctgg cgcacgcccg gcgatgtgct ccagcggagt cgtgcggctt cgtggtaaga
    14821 acgccggagg gggaaagata tttcccctgc gtgaatatct ccggtgagcc ggaggcgtat
    14881 ttccgtatgt cgccggaaga ctggctgcag gcagagatgc agggtgagat tgtggcactg
    14941 gtccacagcc accccggtgg tctgccctgg ctgagtgagg ccgaccggcg gctgcaggtg
    15001 cagagtgatt tgccgtggtg gctggtctgc cggggggcga ttcacaagtt ccgctgtgtg
    15061 ccacatctta ccgggcggcg ctttgagcac ggggtgacgg actgttacac gctgttccgg
    15121 gatgcttacc atctggcggg gattgagatg ccggattttc atcgtgagga tgactggtgg
    15181 cgtcacggtc agaatctcta tctggataat ctggaggcca cagggctgta tcaggtgccg
    15241 ttgtcagcgg cgcagccggg cgatgtgctg ctgtgctgtt ttggttcatc ggtgccgaat
    15301 catgccgcca tttactgtgg tgacggcgag ctgctgcacc atattcctga acaactgagc
    15361 aaacgagaga ggtataccga caaatggcag cgacgcacac actccctctg gcgtcaccgg
    15421 gcatggcacg catctgcctt tacggggatt tgcaacgatt tggccgccgc atcgaccttc
    15481 gtgtgaaaac gggtgccgaa gccatccggg cgatggccac acagctccct gcgtttcgtc
    15541 agaagctgag cgacggctgg tatcaggtgc gtattgccgg gcaggatgtt agcacgtccg
    15601 gattaacggc acagttacat gaggctctgc ctgacggcgc tgtgattcat attgttccca
    15661 gagtcgccgg ggccaagtca ggtggtgtat tccagattgt cctgggagca gccgccattg
    15721 ccggatcatt ctttaccgcc ggagccaccc ttgcagcatg gggggctgcc attggtgccg
    15781 gtggtatgac cggcattctg ttttctctcg gtgccagtat ggtgctcggc ggtgtggctc
    15841 agatgctggc accgaaagcc agaactcccc gcacacagac aacggataac ggtaagcaga
    15901 acacctattt ctcctcactg gataacatgg ttgcccaggg caatgttatg cctgttctgt
    15961 atggtgaaat gcgcgtgggg tcacgtgtgg tatctcagga gatcagcacg gcagacgaag
    16021 gggacggtgg tcaggttgtg gtgattggtc gctgatgcaa aatgttttat gtgaaatcgc
    16081 ctccgggcgg ttttatcgtt tatggagcat gacgaatggg taaaggcagc agtaaggggc
    16141 ataccccgcg cgaagcgaag gacaacctga agtccacgca gctgctgagt gtgatcgatg
    16201 ccatcagcga agggccggtt gaaggtccgg tggatggatt aaaaagcgtg ctgctgaaca
    16261 gtacgccggt gctggacagt gaggggaata ccaacatctc cggcgtcacg gtggtgttcc
    16321 gggcaggtga gcaggagcag tcaccgccgg agggatttga atcctccggc tccgagacgg
    16381 tgctgggtac ggaagtgaaa tatgacacgc cgatcacccg gaccatcacg tcggcaaaca
    16441 ttgaccgact gcgttttacc ttcggtgtac aggcactggt ggaaaccacc tcaaagggag
    16501 acaggaatcc gtctgaagtt cgcctgctgg ttcagataca gcgtaacggt ggctgggtga
    16561 cggaaaaaga catcaccatt aagggcaaaa ccacctcgca gtatctggcc tcggtggtgg
    16621 tggataacct gccgccgcgc ccgtttaata tccggatgcg cagaatgacg ccggacagca
    16681 ccacagacca gctgcagaac aaaacgctct ggtcgtcata caccgaaatc atcgatgtga
    16741 aacagtgcta cccgaacacg gcactggtcg gcgtacaggt ggattcggag cagttcggca
    16801 gccagcaggt gagccgtaat tatcatcttc gcgggcgcat tctgcaggtg ccgtcgaact
    16861 ataatccgca gacgcggcaa tacagcggta tctgggacgg aacgttaaaa ccggcataca
    16921 gcaacaacat ggcctggtgt ctgtgggata tgctgaccca tccgcgctat ggcatgggga
    16981 aacgtcttgg tgcggcagat gtggataaat gggcgctgta tgtcatcggc cagtactgtg
    17041 accagtcggt gccggacggc tttggcagca cggagccgcg catcacctgt aatgcctggc
    17101 tgaccacaca gcgcaaggcg tgggatgtgc tcagtgattt ctgctcggcg atgcgctgta
    17161 tgccggtatg gaacgggcag acgctgacgt tcgtgcagga ccgacagtcg gataaggtgt
    17221 gggcctataa ccgcagtaat gtggtgatgc cggatgatgg cgcgccgttc cgctacagtt
    17281 tcagcgccct gaaagaccgc cataatgccg ttgaggtgaa ctggattgac ccggataacg
    17341 gctgggagac ggcgacagag cttgtggagg atacgcaggc cattgtccgt tacggtcgta
    17401 acgtcacgaa gatggatgcc tttggctgta ccagccgggg gcaggcacac cgcgccgggc
    17461 tgtggctgat taaaacggaa ctgctggaga cgcagaccgt ggatttcagc gtgggtgctg
    17521 aagggcttcg tcatgtaccg ggcgatgtca ttgaaatctg cgatgatgac tatgccggta
    17581 tccgcaccgg cgggcgcgtg ctggcggtga acagccagac ccggacgctg acgctcgacc
    17641 gtgaaatcac gctgccatcc tccggcacca cgctgataag cctggttgac gggcagggga
    17701 atccggtcag cgtggaggtc cagtccgtca ccgacggcgt gaaggtaaaa gtgagccgtg
    17761 ttcctgacgg tgttgctgaa tacagcgtgt gggggctgaa gctgccgacg ctgcaccagc
    17821 gattgttccg ctgcgtgagt atccgtgaga acgacgacgg cacgtatgcc atcaccgccg
    17881 tgcagcatgt gccggaaaaa gaagccatcg tggataacgg ggcgcacttt gacggcgacc
    17941 agagcggcac ggtgaatggt gtcacgccgc cagcggtgca gcacctgact gccgaagtca
    18001 ccgcagacag cggggaatat caggtgctgg cgcgctggga cacgccgaag gtggtgaagg
    18061 gcgtgagctt cctgctccgt ctgaccgtaa cagcggatga cggcagtgag cggctggtca
    18121 gcacggcccg gacgacggaa accacttacc gcttcacaca actggcgctg gggaactaca
    18181 ggctgacagt ccgggcagta aatgcgtggg ggcagcaggg cgatccggcg tcggtatcgt
    18241 tccggattgc cgcaccggca gcgctgtcgc ggattgagct gacgccgggc tattttcaga
    18301 taaccgccac gccgcatctt gcggtttatg atccgacggt acagtttgag ttctggttct
    18361 cggaaaagcg gattgcggat atcaggcagg ttgaaaccac agcacgctat cttggcacgg
    18421 cgctgtactg gatagccgcc agtatcaata tcaaaccggg ccatgattat tacttttata
    18481 tccgcagtgt gaacaccgtt ggcaaatcgg cattcgtgga ggctgttggt cagccgagtg
    18541 atgatgcatc cggttatctg gattttttca aaggcgagat agggaaaacc catctggctc
    18601 aggagctgtg gacgcagatt gataacggtc agcttgcgcc tgacctggct gaaatcagga
    18661 cgtccattac ggatgtcagc aatgaaatca cgcagaccgt caataagaaa ctggaagacc
    18721 agagtgcggc aattcagcag atacagaagg ttcaggttga tacaaataat aacctgaaca
    18781 gcatgtgggc tgtgaagctg cagcagatgc aggacggacg cctttatatc gcgggtattg
    18841 gtgccggtat tgagaacacc cctgacggca tgcagagtca ggtgctgctg gcggcggaca
    18901 ggattgcgat ggttaatcct gcgaatggca acacaaaacc gatgtttgtt ggtcagggcg
    18961 atcagatatt catgaacgac gtgttcctga aacgcctgac ggcccccacc attaccagcg
    19021 gtggaaatcc atcggcattt tccctgactc cggacggaaa gctgaccgct aaaaatgcgg
    19081 atatcagtgg cagtgtgaat gcgaacgccg ggacgctcaa caatgtcacg gtaaatgaaa
    19141 actgtacgat taagggcatg ctggaggcga ctcaggtcag aggtgacttc gttaaagctg
    19201 tatccaaatc atttccgaaa caggctggta cgtggggtaa cacggaaaca ccaaacggga
    19261 cggttacagt caccataagc gatgatcata actttgaccg ccagattatt attccgccca
    19321 ttatttttaa cggtatagcg tatgacgatc cggggagcgg aaataaccca ggaggcacgc
    19381 gatacacggg ttatggtttt gaagttcgca aaaacggcgt attaatcgca tccagagaaa
    19441 ctaaaggtgc cattcccggt agttacagtg cagttattga tatgcctagt ggtggtggta
    19501 gcgtcactct ggagtttaag attttccaga aaggcaatca gggggcaggc aatatcaccg
    19561 actgtacggt gattgtgacc aaaaaagcgg cttccggcat cagtattcgt tgaaatattt
    19621 ataaccccaa taaatggcgt caggaatgac gcctttttta ttgcagaaaa gcgagaggta
    19681 attatgcgta aagtttgtgc agccattttg tccgcagcca tttgcctggc cgtatccggt
    19741 gcgcctgcat gggcgtctga acatcagtcc acgctgagcg cggggtatct tcatgcccgg
    19801 accaacgttc ccggcagtga tgatctgaac gggattaacg tgaaataccg ttatgagttt
    19861 acggatacgc tggggctggt gacgtcattc agctatgcag gagacaagaa tcgccagctg
    19921 acccgttaca gcgatacccg ctggcatgaa gattccgtgc gtaaccgctg gttcagcgtg
    19981 atggcggggc cgtctgtgcg cgtgaatgaa tggttcagcg cgtatgcgat ggcgggtgtg
    20041 gcttacagcc gtgtgtcgac tttttccggg gattatatcc gcgtaactga caataagggg
    20101 aaaacgcacg atgtgctgac cggaagtgat gacggtcgcc acagcaacac gtctctggcg
    20161 cactgacggt ttcatcgtgg gtgtcggtta taaattctga ttagccaggt aacacagtgt
    20221 tatgacagcc cgccggttca ggcgggcttt tttgtggggt gaatatggca gtaaagattt
    20281 caggtgtact gaaagacggc acaggaaaac cggtacagaa ctgcacaatc cagctgaaag
    20341 caaaacgtaa cagcaccacg gtggtggtga acacgctggc ctcagaaaat ccggatgaag
    20401 ccgggcgtta cagcatggac gttgagtacg gtcagtacag cgttattctg ttggtggaag
    20461 gattcccgcc gtcacatgcc gggaccatca ccgtgtatga agattcccga cccggtacgc
    20521 tgaatgattt tctcggtgcc atgacggagg atgatgcccg tccggaggca ctgcgccgtt
    20581 ttgagctgat ggtggaagag gtggcgcgta acgcgtccgc ggtggcacag aacacggcag
    20641 ccgcgaagaa atcagccagc gatgccagca catcagcccg tgaggcggca acccatgcga
    20701 ctgatgctgc agattcagca cgtgcagcca gcacgtcagc cggacaggcc gcttcgtcgg
    20761 ctcagtcagc gtcttccagc gcaggaacgg catcaacaaa ggccactgaa gcctcaaaaa
    20821 gtgctgccgc tgcagagtcc tcaaaaagcg cggcggctac cagtgccggt gcggcgaaaa
    20881 cgttagaaac gaatgcggca gcgtcacaac aatcagcagc cacttctgca tccaccgcga
    20941 ccacgaaagc gtcagaagct gccacctcag cccgggatgc gtcggcttca aaagaggcgg
    21001 caaaatcatc agaaacgaac gcatcatcaa gcgccagtag cgcagcttcc tcggcgacgg
    21061 cggctgcaaa ttctgcgaag gcggccaaaa cgtccgagac gaacgccagg tcttctgaaa
    21121 cggcagcggg acagagtgcc tcagctgcgg caggctcaaa aacagcggct gcgtcgtctg
    21181 ccagcgccgc gtcaacaagt gccgggcagg cctcagccag tgccaccgcc gccggaaaat
    21241 cggcagaaag cgccgcatca tccgcttcaa cagccacaac gaaggctggc gaagccactg
    21301 agcaggccac ggcagcagcg aggtcagctt ccgcagcgaa gacatccgaa acgaacgcga
    21361 aagcgtcgga aacccgtgca gaatcctcaa aaacggctgc cgcatcgtcc gccagttcgg
    21421 cggcgtcatc ggcatcatcg gcgtctgctt caaaagatga ggcgaccaga caggcgtcag
    21481 cagcgaaggg cagcgccacg acggcatcca cgaaggcgac agaggctgct ggtagtgcga
    21541 cggcagcagc tcagagcaaa agtacggcgg aatctgcagc aacgcgcgct gagacagcgg
    21601 caaaacgggc agaggatatt gcatccgccg tggcgcttga ggatgcgagc acgacgaaaa
    21661 aggggatagt acagctcagt agtgcgacta acagcacttc cgagtcactg gcggcaacac
    21721 caaaagccgt taaggccgcg tatgacctgg ctaacgggaa atacaccgca caggatgcaa
    21781 cgacagcaca gaaagggata atccagctaa gcagcgcgac caacagcacg tctgaaacgc
    21841 tggcggcaac gccaaaggca gtaaaagcag ccaatgacaa tgctgagaaa cgtctgcaga
    21901 aagatcagaa cggtgcggat atccctggca aagacacctt tacgaaaaat attggtgcct
    21961 gccgtgcctt cggtgggtca gtaagcacaa caacaggaaa ctggacgact gcacagttta
    22021 tcgagtggct ggattctcag ggagcattta accatccata ctggatgtgc aagggttcct
    22081 ggtcttatgg caataataaa atcattactg atactggctg cggtaatatt catctcgccg
    22141 gagctgtcat tgaagtaatg gggataaagt cagcgatgac gatccgcatt accactccga
    22201 ccacatccag cggtggcgga acaaccaacg cccagtttac ctatattaat cacggaacag
    22261 attattcacc tggctggcga agggactata actccagaaa taagccaacg gcatcagaga
    22321 tcggggcgct accgtcaggt ggaacagcag tatcatcagt taatctggct tcaaaaggtc
    22381 gggtagccgc gctgacagac aatacgcagg gggcagcagg tcttgagtta tacgaggtgt
    22441 ataacaacgg atatccaaca gcgtatggaa atatcattca cctgaaaggg atgacagccg
    22501 ttggcgaagg tgagttactc atcggctgga gtggtacaag cggtgctcat gctccggcat
    22561 ttattcgttc acgacgggat acgaccgacg caaactggtc gccgtgggcg cagctttaca
    22621 cctcggctca tcctcctgca gagttttatc cagtcggtgc accaatcccg tggccatcag
    22681 ataccgttcc gtctggttat gccctgatgc aggggcagac ttttgacaaa tctgcttacc
    22741 cgaaacttgc agccgcttat ccgtcaggcg tgatccctga tatgcgtggc tggacgatta
    22801 agggcaagcc cgccagtggt cgtgccgtat tgtctcagga acaggatggc attaaatcgc
    22861 acacccacag cgccagcgca tccagtacgg atttggggac gaaaaccaca tcgtcgtttg
    22921 attacggcac taaatccacg aataacaccg gggcgcatac gcacagtctg agcggctcta
    22981 caagttcagc tggggcccac caacatagtc agactgggcc cagaacgaat agcgggagtc
    23041 aacctactgg aatgtttccg gcagggagta ctcaagttag cgggactaat caggttggta
    23101 tcagtggctc attaaccagt ggcacctccc aatgggtagg taaatcatct tcagaaggaa
    23161 accatacaca ctcactgtcc ggcacagcag catctgcggg tgcacacgcg catactgtcg
    23221 gtattggtgc tcatacgcac tctgttgcga ttggttcaca tggacacacc atcaccgtta
    23281 acgctgctgg taacgcggaa aacaccgtca aaaacatcgc atttaactat attgtgaggc
    23341 tcgcataatg gcattcagaa tgagtgaaca agcacggacc ataaaaattt ataacctgct
    23401 ggccggaact aatgaattta ttggtgaagg tgacgcatat attccgcctc atacaggtct
    23461 gcctgcaaac agtaccgata ttgcaccgcc agatattccg gctggtttcg tggctgtttt
    23521 caacagtgat gagtcatcgt ggcatctcgt tgaagatcat cggggtaaaa cggtttatga
    23581 cgtggcttcc ggcgacgcgt tatttatttc tgaactcggc tcattaccgg aaaatgtcac
    23641 ctggttatcc ccggaagggg agtttcagaa gtggaacggc acagcctggg tgaaggatac
    23701 ggaagcagaa aaactgttcc ggatccggga ggcggaagaa acaaaaaaca gcctgatgca
    23761 ggtagccagt gagcatatag cgccgcttca ggatgctgtg gatctggaga tcgcaacgga
    23821 gaaagaaacc ttgttgctgg aagcttggaa aaagtatcgg gtgttgctga accgtgttga
    23881 tacatcaact gcaccggata ttgaatggcc agtagcacct atagggtaaa ttcgtaatga
    23941 ttacctaaat acgttattct tttgttaaaa aagtgatttt gctataggta gggagtatgt
    24001 tcttggatta agaataaaaa atcgccatga aagagcttca tggcgagtac ataaacgtac
    24061 aaaatgtgat gaaagtagag tggcgtaagc cactatatta agatatgaca atagcgaaga
    24121 aaagtaaata acattttatg gacgtttaat tgttaattat aaaaactaga gtgcccgata
    24181 ttgtttatct tgctttaaaa taatgtactc aaaccaaatt tttgagctaa aagctcaaat
    24241 aatataaatg caataataat gtttaataag acaattagct tgctttgtat tttcatttcg
    24301 gaatactgtt aagtaacttt gatgacccta acaatagatc ggaattttct tagagtcaat
    24361 ggtcactgtt tttgcttgca aagcaaatgt tttggtggtt ataagtcgtc ttaatatagt
    24421 cacgcatgca acttgttcca tgcggagatt aaagattgga atataacgcg aagatggaat
    24481 taaattacat ttgcaataat taatttatta ttactcaata attaccgata ttattattgt
    24541 aaattaatca ttagaatgca aaaagaaatg taacggcaaa ataagaggac gatagctgag
    24601 aaaaaagcgt gctttcccaa tctggggcaa atcacgtctg aatgacacct gaaaacaggt
    24661 ggta
//
LOCUS       DAJTYZ010000057        24576 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000057 DAJTYZ010000000
VERSION     DAJTYZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.057"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..588)
                     /locus_tag="OO596_002165"
     CDS             complement(<1..588)
                     /locus_tag="OO596_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6718014.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRF"
     gene            1155..1520
                     /locus_tag="OO596_002166"
     CDS             1155..1520
                     /locus_tag="OO596_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6718015.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1560..2216
                     /locus_tag="OO596_002167"
     CDS             1560..2216
                     /locus_tag="OO596_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR6718016.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2283..3701
                     /gene="dcm"
                     /locus_tag="OO596_002168"
     CDS             2283..3701
                     /gene="dcm"
                     /locus_tag="OO596_002168"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6718017.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3682..4152
                     /gene="vsr"
                     /locus_tag="OO596_002169"
     CDS             3682..4152
                     /gene="vsr"
                     /locus_tag="OO596_002169"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6718018.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4141..5062)
                     /gene="yedA"
                     /locus_tag="OO596_002170"
                     /pseudo
     CDS             complement(4141..5062)
                     /gene="yedA"
                     /locus_tag="OO596_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5235..6152
                     /locus_tag="OO596_002171"
     CDS             5235..6152
                     /locus_tag="OO596_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6718019.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6231..6413
                     /locus_tag="OO596_002172"
     CDS             6231..6413
                     /locus_tag="OO596_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6718020.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6584..8278
                     /gene="yedQ"
                     /locus_tag="OO596_002173"
     CDS             6584..8278
                     /gene="yedQ"
                     /locus_tag="OO596_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR6718021.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8275..9090)
                     /locus_tag="OO596_002174"
     CDS             complement(8275..9090)
                     /locus_tag="OO596_002174"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCR6718022.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9388..9615)
                     /gene="yodD"
                     /locus_tag="OO596_002175"
     CDS             complement(9388..9615)
                     /gene="yodD"
                     /locus_tag="OO596_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6718023.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9778..9966
                     /gene="dsrB"
                     /locus_tag="OO596_002176"
     CDS             9778..9966
                     /gene="dsrB"
                     /locus_tag="OO596_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6718024.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10010..10633)
                     /gene="rcsA"
                     /locus_tag="OO596_002177"
     CDS             complement(10010..10633)
                     /gene="rcsA"
                     /locus_tag="OO596_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6718025.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10918..11703)
                     /gene="fliR"
                     /locus_tag="OO596_002178"
     CDS             complement(10918..11703)
                     /gene="fliR"
                     /locus_tag="OO596_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6718026.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11712..11981)
                     /gene="fliQ"
                     /locus_tag="OO596_002179"
     CDS             complement(11712..11981)
                     /gene="fliQ"
                     /locus_tag="OO596_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6718027.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11991..12728)
                     /gene="fliP"
                     /locus_tag="OO596_002180"
     CDS             complement(11991..12728)
                     /gene="fliP"
                     /locus_tag="OO596_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6718028.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12728..13093)
                     /gene="fliO"
                     /locus_tag="OO596_002181"
     CDS             complement(12728..13093)
                     /gene="fliO"
                     /locus_tag="OO596_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6718029.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13096..13509)
                     /gene="fliN"
                     /locus_tag="OO596_002182"
     CDS             complement(13096..13509)
                     /gene="fliN"
                     /locus_tag="OO596_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6718030.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13506..14510)
                     /gene="fliM"
                     /locus_tag="OO596_002183"
     CDS             complement(13506..14510)
                     /gene="fliM"
                     /locus_tag="OO596_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6718031.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14515..14979)
                     /gene="fliL"
                     /locus_tag="OO596_002184"
     CDS             complement(14515..14979)
                     /gene="fliL"
                     /locus_tag="OO596_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6718032.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15084..16211)
                     /gene="fliK"
                     /locus_tag="OO596_002185"
     CDS             complement(15084..16211)
                     /gene="fliK"
                     /locus_tag="OO596_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6718033.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16208..16651)
                     /gene="fliJ"
                     /locus_tag="OO596_002186"
     CDS             complement(16208..16651)
                     /gene="fliJ"
                     /locus_tag="OO596_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR6718034.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16670..18043)
                     /gene="fliI"
                     /locus_tag="OO596_002187"
     CDS             complement(16670..18043)
                     /gene="fliI"
                     /locus_tag="OO596_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6718035.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18043..18729)
                     /gene="fliH"
                     /locus_tag="OO596_002188"
     CDS             complement(18043..18729)
                     /gene="fliH"
                     /locus_tag="OO596_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6718036.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18722..19717)
                     /gene="fliG"
                     /locus_tag="OO596_002189"
     CDS             complement(18722..19717)
                     /gene="fliG"
                     /locus_tag="OO596_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6718037.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19710..21368)
                     /gene="fliF"
                     /locus_tag="OO596_002190"
     CDS             complement(19710..21368)
                     /gene="fliF"
                     /locus_tag="OO596_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR6718038.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21584..21898
                     /gene="fliE"
                     /locus_tag="OO596_002191"
     CDS             21584..21898
                     /gene="fliE"
                     /locus_tag="OO596_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6718039.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22232..22564
                     /gene="emrE"
                     /locus_tag="OO596_002192"
     CDS             22232..22564
                     /gene="emrE"
                     /locus_tag="OO596_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6718040.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22733..23284
                     /gene="ybcL"
                     /locus_tag="OO596_002193"
     CDS             22733..23284
                     /gene="ybcL"
                     /locus_tag="OO596_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6718041.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23294..24091
                     /locus_tag="OO596_002194"
     CDS             23294..24091
                     /locus_tag="OO596_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6718042.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         6030 a         6080 c         6300 g         6166 t
ORIGIN      
        1 gaagcgaacg tcttcgtgac catttttagt accttcttga tcttctcctg cattgctgcc
       61 ttcgttgtta ccttgatact gcaatgcaaa gttcagacct tccaccagac cgaagaaatc
      121 actattgcgg taagtagcta ccccatttgc acggcctgcc atgaagttat cagtccaggt
      181 ataggagtcg ccgccgaata caggaagcat atctgtccag gcttcaacgt cgtaaatcac
      241 accatagtta cgaccatagt caaaggagcc catctcgccg aacttcagac ctgcaaaagc
      301 aagtttgaca taagtattat cagaaccttc agtgtcgcta gcctttgttt tggtcagtgc
      361 ttgaccataa ccagttaact gatctgtaat ttgggtctca cctttgaagc ctaactcgat
      421 gatagtttca tcgccgtcat cgccagggtt atcggaaaaa gtatgacgag catcaacttt
      481 tccgtacaga tccagtttat tgccattttt attataaatt tcagccgcat ttgctgcgcc
      541 agcaactaat aacgctggga ccagcattgc cagaactttt cttttcatta tgtattccct
      601 tgtgattata atcttcatga atatatcaat aagtgccgtt atccaaaaaa aaagcacatt
      661 tggatactat tctatgaagt tcattttatt ttaaagattg cgtgtaacaa atgtatttaa
      721 aagatttcaa aatgtttctt atctatttga tataattcag aattatgaat tctaaatata
      781 aacatccatg aaaattataa atgtatatca atatgttatg agttgaatgg aaagataggg
      841 agtatcaatg attgttgcgc caaacggcag gcgtgatcac gcgcacatta atgaaggtat
      901 aaaaaaagcc ggcatcatgc cggcccaaaa aatataaaca agacgataaa aggctaataa
      961 cggggttatc atgacacaga aatcaaaaaa ggtataattg tattcagtgc taatatattg
     1021 tttgggtgaa ttaataaaac tattaccaag gatataaata aatctttatt gctgtttttc
     1081 cagtaattat tgccgttgcg caaaatttat ctatttgttc aaaaaatgat tgagtcttga
     1141 ctggctcatc caatgtggaa aaatgtgact tttatcacat aatagtacta agtctgaatt
     1201 ttccgggtta tctcaaaatg gaatacggtt cgacaaagat ggaagagaga ctctctcgca
     1261 gccctggcgg caaattggct ttgtgggctt tctatacatg gtgtggctat ttcgtctggg
     1321 caatggcgcg ttacatctgg gtgatgagcc ggatccctga tgccccggtg tcgggatttg
     1381 aaagcgattt aggctctacc gcgggaaagt ggttaggggc actggtcgga tttttattta
     1441 tggctctggt cggggcattg ttaggcagca ttgcctggta cacccgacct cgtcccgcac
     1501 gtagcagacg ctatgaataa aaactgctaa ggtggcgact ctttggcaac ggagcgcgta
     1561 tggacttaca acactggcag gcacagttcg aaaactggtt gaaaaaccat catcagcacc
     1621 aggacgcggc acagaagctg gcggcagatg acgatgtcga tatgctggtg attttaaccg
     1681 cctgttattt tcacgatatt gtgagcctgg cgaaaaatca tccgcaaagg cagcgttctt
     1741 caatcctggc ggcagaagag acgcgtcgcc tgctccgtga agagttcgag caatttccgg
     1801 cggagaaaat cgaggccgtt tgtcatgcca ttgccgctca cagtttcagc gcgcaaatcg
     1861 cccccttaac aacggaagct aaaatcgtcc aggatgcgga tcggctggaa gccctggggg
     1921 cgattggcct ggcgcgcgtg tttgccgttt caggggcatt gggcgtggcg ctgtttgatg
     1981 gtgaagatcc gttcgcacag catcgcccgc ttgatgataa acgctacgcg ctggatcatt
     2041 tccagactaa gttgctgaag ctaccgcaaa ccatgcaaac cgcaagaggc aagcagctgg
     2101 cgcagcacaa cgcgcatttt ttagtcgagt ttatggcgaa gctcagtgcc gaactggcgg
     2161 gggagaatga aggtgtcgat cacaaggtga tagatgcgtt ttcacccgcg ggataagcgc
     2221 gtggccctaa atggctgtaa ttatgttaac ctgtcggcca tctcagatgg ccggttaaat
     2281 ctatgcagga aaatatatca gtaaccgatt catacagcac cgggaatgcc gcacaggcaa
     2341 tgctggagaa actgctgcaa atttatgatg ttaaaacgct ggtggcgcag cttaatggtg
     2401 tgggtgagaa tcactggagc gcggcaattt taaaacgtgc gctggcgaat gactcggcat
     2461 ggcaccgttt aagtgagaaa gagttcgccc atctgcaaac gttgttaccc aaaccatcgg
     2521 cacatcatcc gcattatgcg tttcgcttta tcgatctatt tgccggaatt ggcggcatcc
     2581 gtcgcggttt tgaatcgatt ggcggacaat gcgtgtttac cagcgaatgg aacaaacatg
     2641 cggtacgcac ttataaagcc aaccattatt gcgatccggc gacgcatcat tttaatgaag
     2701 atatccgcga catcaccctc agccataaag aaggcgtgag tgatgaggcg gcggcggaac
     2761 atattcgtca acacattcct gaacacgatg ttttactggc cggtttccct tgtcagccat
     2821 tttcgctggc tggcgtatcg aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg
     2881 atacccaggg cacgctgttt tttgatgtgg tacgcattat cgacgcgcgt cgtccggcga
     2941 tgtttgtgct cgaaaacgtc aaaaacctga aaagtcacga ccagggtaaa acgttccgca
     3001 tcatcatgca gacgctggac gaactgggct atgacgtggc tgatgcagaa gataacgggc
     3061 cggacgatcc gaaaatcatc gatggtaaac attttctgcc gcagcaccgt gaacgcatcg
     3121 tgctggtggg ttttcgtcgc gatcttaatc tgaaagccga ttttactctg cgtgatatca
     3181 gcgaatgttt ccctgcacag cgagtgacgc tggcgcagct gttggacccg atggtcgagg
     3241 cgaaatatat cctgacgccg gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc
     3301 aggcgcgcgg taacggcttc ggttatggaa tggtttatcc gaacaatccg caaagcgtca
     3361 cgcgtacgct gtctgcgcgt tattacaaag atggcgcgga aattttaatc gatcgcggct
     3421 gggatatggc cacgggtgag aaagattttg acgatccgct gaatcagcaa catcgtccac
     3481 gtcggttaac gcctcgggaa tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga
     3541 aattccgcat tccggtttcg gacactcagg cctatcgcca gttcggtaac tcggtggtcg
     3601 tgccggtctt tgccgcggtg gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt
     3661 tgcgtcagca agaggcacaa catggccgac gttcacgata aggccactcg cagcaaaaat
     3721 atgcgcgcga ttgccacgcg tgatacggcg attgagaagc gcctcgccag tctgttaacc
     3781 gggcagggcc tggcgtttcg cgttcaggac gccagtctgc ccggaagccc ggattttgtc
     3841 gttgatgaat atcgctgcgt gatatttacc catggctgct tctggcatca tcatcactgc
     3901 tatctgttta aagtgcctgc gactcgaacc gagttctggc tggagaagat aggtaaaaat
     3961 gttgaacgcg atcgccgcga tatcagtcgt ttgcaggaac tcggctggcg cgtactgatt
     4021 gtctgggagt gtgcattacg tgggcgcgag aagctgacgg atgaagcgct taccgagcgt
     4081 ctggaagagt ggatctgcgg cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat
     4141 ttactcgctt gatgcgtcct gaataactgg cgcaactacg ggttttgccg ggaagagata
     4201 ttttcccaac gtgaccagta ccaccgcgaa gacaattacg ccgagcgcca gccattcaat
     4261 cttcgacagc gtctctccgc ccagtcccgt acccagcaag accgcgacca ccgggttaac
     4321 gtaggcgtag ctggtggcga gagccggact gacattacgg attaaataca tataagcgtt
     4381 gatggcgata atcgaaccaa acagcgccag atagccgacc gcaaggaagc ctgaaaggga
     4441 agggagcgcc gtcagttttt cacctgcaat catcgacgcg atcattaaca ccacgcctgc
     4501 cgccagcatc tcaatcgcac ccgccatcat ccctacaggt aaggtaatgc gcgagccata
     4561 aaccgagcca aacgcccagc taatcgagcc gattaaaatc agaatcgcgc cccacggatt
     4621 gccgcttaaa tttccaccgc tattgagcat gatgattccg gcaagcccaa tggcgatacc
     4681 cacccattcc agtttgcgcg ttttaatgcc aaacaggcgg ctgaagcacc agggtaaaga
     4741 ggggcacggt tgcaaccaca acggcggcga tgccggaagg aacattttga tgctcggcaa
     4801 ccgtcaccat gccattaccg acagccagca ataacaggcc aatcagcgcg gcattgagca
     4861 gcggacgtag cggggggagc ttgtgtccgc gcagtagcaa aaatgccatc aataaaatac
     4921 cggctgccag gaatcgaacg cctgccatca ttaacggagg ccagctttcc acgccaatcc
     4981 gaatgacaaa ataggttgag ccccaaatga tatacaacgc aaacagcgcg ccaaaaagcg
     5041 gtaacaactg gcggaaacgc ataatccctc acggtggaaa taaaaaggtg gttcatagta
     5101 aacgtgaaaa tcattctgct ggcgagagat ataattgcac ttgattgtaa aaaaaatgtt
     5161 gacctgtgaa gcaagtatca gagctgccgt ttttgcttca tacttacacc cttcaacaat
     5221 aaaaatgaga gggaatgctt ttggccggga gtagtttact gacgttgctc gatgatatcg
     5281 ccacactact ggacgatatc tccgtgatgg gcaaactggc ggcgaagaaa accgccggtg
     5341 tattagggga tgacttatcg ctcaatgcgc aacaagtttc aggcgtgcgg gccaaccggg
     5401 aacttcccgt ggtctggggc gtggcgaaag gatcgctgat taataaagtg attctggtgc
     5461 cgctggcgct gatcatcagt gcgtttatcc cgtgggcgat tacgcctctg ttgatgattg
     5521 gtggcgcgtt tctctgcttt gaaggagtag agaaagtgct gcatatgctg gaggcgcgta
     5581 aacataaaga agatccggcg cagagccagc agcgtctgga gaagctggcg gcgcaggatc
     5641 cgctgaagtt tgaaaaggac aaaataaaag gggcgattcg taccgatttt atattgtctg
     5701 ccgaaatcgt cgccatcacg ctggggattg tggccgaagc gccgttgctt aatcaggtgc
     5761 tggtgctttc aggcatcgcg ctggtagtga ccgtgggcgt ctacggtctg gtaggggtta
     5821 tcgttaagat tgatgacctg gggtattggc tggcggaaaa atccagcgcg ctgatgcagg
     5881 cattaggtaa agggttattg atcattgcgc cctggttgat gaaggcgtta tcgattgtcg
     5941 gcacgctggc gatgttcctc gtcggcggcg gtattgtggt acatggtatt gcgccgctgc
     6001 atcacgccat tgaacatttc gccgggcagc aaagtgcagt ggtggcgatg atattaccga
     6061 ctgttttaaa tctgattctt ggatttatca tcggcggcat cgtggtgctg ggagtgaaag
     6121 ccgtagcgaa aatgcgcggt caggcacatt aatactcgta caggctatta tgcaaaattc
     6181 gccatcttca actaaggtga acaagtttca ctcgaaaaaa ggaggcgagt atgagcttta
     6241 tggttagtga ggaagttacg gttaaagagg gcggcccgcg gatgattgtc actggatact
     6301 ccagcggtat ggttgagtgt cgttggtatg acggttacgg ggtcaagcgg gaagcttttc
     6361 atgaaaccga gcttgttccg ggggagggga gtcgttctgc ggaagaagtt tgaaagggta
     6421 tcattaacac caggccggat cacttccggc ctggtgtgcc aatgcctgca atgaatgata
     6481 gtcgcgtctt atcatgccac cattataaaa ttatcgctgt tttgcggtac gctatcgcca
     6541 ggttatgcca gaatcataaa aaagcaggtt gggagtcgtc agggtgcagc acgagacaaa
     6601 aatggaaaac cagagctggt tgaaaaaact cgcacgccgc ctggggcctg gtcatgtcgt
     6661 taatctctgc tttatcgtgg tattgctttt ttccaccttg ctgacctggc gtgaagtggt
     6721 agtgctggaa gatgcctata tctccagcca gcgtaatcat ctggaaaacg tagccaacgc
     6781 gctcgataag catttgcagt ataacgtcga caaactgatc tttttgcgta atggcatgcg
     6841 cgaagctctc gtagcgccac tggatttcac ttcactgcgt aatgctgtaa ccgagttcga
     6901 acagcatcgc gacgagcacg cctggcaaat tgaactcaac cgacgacgca ccctgtcagt
     6961 caatggcgta tcggatgcat tagtcagcga ggggaatctc ctgtctcgcg aaaatgaaag
     7021 cctcgacaat gaaattaccg ctgcactgga agttggttac ttgctgcgac tggcgcacaa
     7081 cacctcgtcg atggttgaac aggcgatgta tgtctcgcgt gccggatttt acgtttcgac
     7141 gcagccgacc ttgtttacgc gcaatgtacc aacgcgttat tacggctatg tcacccaacc
     7201 ctggtttatc ggccattcgc aacgagaaaa tcgtcaccgc gcggtacgct ggtttacttc
     7261 gcaaccggag cacgccagca atactgaacc gcaggttacc gtcagtgttc cggtagacag
     7321 taataactac tggtatggcg tgctggggat gagtattccc gtgcgtacca tgcagcaatt
     7381 tttaagaaac gccatcgata aaaacctcga tggtgagtat cagctctatg acagtaagct
     7441 gagatttttg acctcttcca atcctgacca tccaacaggg aatatttttg atcctcgtga
     7501 actggccttg ctggcgcagg cgatggaaca tgacacgcgg ggcggcattc gtatggacag
     7561 tcgctatgtt agctgggaac gtctggacca tttcgacggt gtgctggtgc gcgtccatac
     7621 gctaagcgaa ggcgtgcgcg gcgatttcgg cagtatcagc attgcattaa ccctgctgtg
     7681 ggcgctcttt accaccatgt tactcatctc ctggtatgtg attcgccgga tggttagcaa
     7741 catgtatgtt ctgcaaagct cgttgcagtg gcaggcgtgg cacgacacct taacccgttt
     7801 atataatcgt ggcgcactgt ttgaaaaagc ccgtccgctc gcgaaattgt gtcagacgca
     7861 ccaacatcct ttttctgtca ttcaggtcga ccttgaccat tttaaagcga ttaatgaccg
     7921 ctttggtcat caggcgggcg accgtgttct ttctcatgct gccggattaa ttagcagttc
     7981 cttgcgtgcg caggacgttg ccgggcgggt cggtggtgag gagttttgtg tgattctgcc
     8041 aggcgcgagt ctgacggagg ctgcggaagt cgcagaacgt attcgcctga agttaaatga
     8101 aaaagagatg ttgattgcta agagtacgac gatacgcatc agtgcctcgt tgggggtaag
     8161 tagcagcgag gaaaccggtg attatgattt tgaacaactc cagtcactgg ctgaccgtcg
     8221 gctttatctc gctaaacagg ccgggcgtaa tcgggtatgc gcgagcgata acgcttaacg
     8281 agcggagaaa aaatggtcca gcccttcacg ccagccttcc ggtccttcac gctgcgttcg
     8341 ccagacgcgg gtcggatcct catcatgcag atgcacccct tcacggttta gccctttcac
     8401 aatcaccgcg taatccatta cctccagtaa gggcgcatcg tttggcccat cgcccaggcc
     8461 aagtgtggtt gggcgtttgc ctgataattg ttgataggtc gcgataatcc agttggcggc
     8521 ctgatctttt ccggcagagg catccaggac gtgccagaag cgcgcaccct gcataaactg
     8581 taagcccagt tcgttcagac gagcggtaaa ttgtgccata cgctcgtcac tgtcgcgcca
     8641 gattagcgtt accgacgcct catgcagctg cgtcagcgcc gcctggctac ggcttaatcc
     8701 cgtccattcg gcgatggttg catcgtcgac atcatcgaaa gtcgtgaatt taaaatgttc
     8761 tttctcgcgt agcgtattta aaacctggct gatttcgcca tggctaatac ctgagatgat
     8821 gcgtggaaaa ccgtctatat cctgccattg ttcagcaagc tggatcactg cgccattttc
     8881 ggcaatcagt ggtaaccctt gtagccccaa cgttttttgc aagtacagca tttccgctga
     8941 agttttactg ctacagagaa tgacgggaac attcgcttcg cgtaaacggc tgagccagag
     9001 ggcagtcggt tgccagtcat aactatgact gtccagcagg gtgccatcaa gatcgctaaa
     9061 aaccagtagt ggttgttgaa ttgaaaacat ggtcgtgcag ttctcccctt accaggatga
     9121 ttctcaaaac agcacaaata atcaaagatg attttttcgg gagcgatccc gttgacgcca
     9181 gagccaccaa ggggtgacgc acgtcacatt tctattcata agtagcgtta attattcata
     9241 tcgcgaatat tttcttgtca gcgaaaaaaa ttgcggataa ggtgatgaac acatcagatt
     9301 tcctggtgta acgaattttt taagtgcttc ttgcttaagc aagtttcatc ccgaccccct
     9361 cagggtcggg atttttttat tgtgcattca acgattcact tcagagacgc tgaagtcatg
     9421 aatatccagt tcgaaggcat ccgccaattc acgccatgta tgatattcac gtccatcgac
     9481 gctaacgtgt tcagaatcgt tctggctgcg atgaacttcg ctttcgctaa tttcattgat
     9541 cgccgccagc agggcatcga catcgacgct gacctcacgt tttgcggtat cgctgtactc
     9601 ttttgcggtt ttcatcatct ttcctcgcaa ccgttactct gttgttgccg ccagtcgtta
     9661 agtccgcctg gcatctctta agtatagacc gtcgagaaaa tcagcactcg cgcgcgcctg
     9721 gagctgccag aaaaacgaaa ttgttctaca ctggcacaaa gccacaggag gaaaacgatg
     9781 aaggtgaatg atcgggtaac agtcaaaacg gatggcggtc cgcgtcgtcc tggcgtggta
     9841 ctggcagttg aggagtttag tgaaggcaca atgtacctgg tttcgctgga agactacccg
     9901 ctcggcatct ggttctttaa tgaagcaggg catcaggacg gtatctttgt ggagaaagca
     9961 gagtaatctt attcagcctg actggtggga aaccaccagt cagaatgtgt tagcgcatgt
    10021 tgacaaaaat accattagtc acattatccg tcagtcggac gacatggtag ataacctgtt
    10081 tattatgtgt tttgatctta cgtttaatat tacctttatg cgatgaaacg gtcttggctt
    10141 tgatattcat ttggtcagag atttgaatgg ttccctgacc tgccatccac attcgcaaca
    10201 tactcgattc ggttcggctc aatgataacg tcggcatatt taaaaaagag gttatcgttg
    10261 tctctttttt cagaatatcg ccaaggatat cgtcgagaga ttccggttta atcgatttag
    10321 aacttatcaa taattttttt ctgaccaata gatattcatc aaaatgaaca ttggcaattg
    10381 ccataaaaac gataaataac gtattgggat gttgattaat gatgtgcttg atgtgctgac
    10441 tgttagaagc atcatggatg aaacagtcct cattaataaa caccactgaa gggcgctgtg
    10501 aatcacaagc tatggcaagg tcatcaacgg tttcaatgtc gttgatttct ctttttttaa
    10561 cccctctact caacagatac ccggttaaac ctagtcgggt gtaactacat aaatccataa
    10621 taatcgttga catggcatac cctcactcaa tgcgtaacga taattcccct tacctgaata
    10681 tttcatcatg actaaacgga acaacatggg tcacctaatg cgccactctc gcgatttttc
    10741 aggcggactt actatcccgt aaagtgttgt ataatttgcc tggaattgtc ttaaagtaaa
    10801 tgttgcgata tgtgagtgag cttaaaacaa atatttcgcc gcaggagtat cctggaagat
    10861 gttcgtaggt attatttttc ggataatcct taggataaca tgataaacgt tacggaatta
    10921 tattaatggc aattcactaa taatatcagc cagcaaatta aacatctcac tgaataaatg
    10981 ttcgcaaaaa ggtgcaatta acggcattaa tgccgccatt aaagagatac cgacagtcag
    11041 agttaatgga aatccaataa caaaaatgga taattgcggg gccatacgat tcagtaaacc
    11101 taatgccaga ttcagggtca gcagcagagt aatgagcggt aacgccagca tcagcccgtt
    11161 aaggaaaatc aaactccccg ctttggtgag tgccagaaac gcattgctgt tcaacggttc
    11221 gccaccaatt ggcagggtgt gaaaggtatc gaccagcagt gaaatcaacc ataaatgacc
    11281 gttaaatgtc aggaacagca gtaacgccag catatccatg atacgcgcta aaacgggcat
    11341 attgagatgg ctggccggat cgacaaacgt cgcaaatgac agccccattt gcagaccgat
    11401 aatttcgcca gcggttcgca cagcggcaaa ggcaaattgc atggtaaaac caagcgcaat
    11461 gccgatcagg atctgctgca cggccagcca cagagcaaag aacgaaaaaa caggaacatc
    11521 gttggcaggt aatgatgggg caatgacgaa cgtgatcatc attgccagcc ccagttttac
    11581 ccgtttcggc acgctgcgtt cgctcagaat tggtgcggta gaaatgagcg ccagcacgcg
    11641 cagtaacggc cagaagtaca ggcttaacca ggaaagccat tgatcgcttg tcacctgcat
    11701 cattgtacgg cctacccgat gatatacggc aggttagtga acaaggtgcg gacgtaatcc
    11761 agcaacagat tgagcatcca cggtccggca ataataatgg caataaatac ggcgatgatt
    11821 tttggaataa acgacagcgt catttcgttg atctgcgtgg cggcctgcaa aatactgatg
    11881 ataaggcccg tgaccagcgc caccagcaac agcggggcag ccagtgccag cgcgactttc
    11941 atcgcttcag tccccatcat catgaccgat tcaggtgtca ttttgcctct ctagctgtaa
    12001 aagctctgcg ccagcgaacc gaccagcaat tgccagccat ccaccagtac aaacagcatc
    12061 agtttaaagg gcagagcaat ggtggcgggg gggaccatca tcatccccaa tgccatcaac
    12121 acgctggcaa tcaccaggtc gataatcaaa aaaggaatga aaatcgtgaa gcctatctga
    12181 aatgcggttt tcaattcgct ggtcacgtag gccgggagca aaatgcgcat cggcacggct
    12241 tcaggtccct gcaacgggcc ggtattcgct agtctggcaa acaaccctaa atctgcctca
    12301 cgggtctgac gcagcataaa ctcacgtagc ggctgcgccc ctttttccag cgcctcctgc
    12361 attgatattt tctcttcgct gaatggctgg tacgcatcga cataaatttt gtcgatcacc
    12421 gatgacataa taaaaaaggt caaaaatagt gccagcccca gcaatacctg gttaggtggc
    12481 gcggagggcg ttcccagcgc gttacgcaat aaaccaaaaa caatgatgat gcgggtgaaa
    12541 ctggtcatca tcagtaaaat tgccggaata aacgtcaacg aggtgatgaa caccagcgtc
    12601 tgcaccggga gcgaccagct ttgtccaccg ccaggcagcg gctggctggt gatacccggc
    12661 agttgcgcga aggcgagggg cgtaaccagc cagagaagga caggtgcgac agacaacaaa
    12721 cgacgcatca ggatctccca ctacgcttaa gcaaattttt catgaccgac tgaaaatcgg
    12781 tctgcggtat ctcttctgtt ggtgctgaag ggggaagttt atgcagcaga ttgatttgcc
    12841 ctgcggtaac gccgagcacc agccgtgcat cttccacatc gaccaccaca acccgttcac
    12901 gcgcgcccag cgaagcactg gcgctaattt tcagaccgtt aacgccagtg cgtttcgggg
    12961 caaatcccaa ccgttttacc agccaggcag cagcgaggat cagggcaata atggcgatga
    13021 gtgcgccgct gacctgcagc agtggcgcag cagaaaccgg cgtggaagat tgcacagtag
    13081 cgtggttatt catcactaac ggctcaggcg acgcattcgc tcagacggag taatgatatc
    13141 ggtgatccgc acgccatatt tatcggcaac gaccaccact tcgccctggg agattaaata
    13201 accgttgatc agaatatcca gtggttcgcc cgccagaccg tccagcgcca cgacggaccc
    13261 ttgcgtcaga cgcaacagct ctttgatggt catccgcgta cggcccagct cgacggtcag
    13321 cttgaccgga atatccatta tcaggtcgat atcctgcaac gttccgctga catcaccacc
    13381 gccaaattgc tggaacaccg cgtcggcagc gcttttgctg ctggttgatt tttgttcgct
    13441 caacgcttca gcccacagat cgtccattgc gccgttgttg tcatcggccg gattattcat
    13501 gtcactcatt tgggctgttc ctcgttcaga gaatttaaaa tcgggttaat caaatgttct
    13561 atccgtaacg catactgacc gttgagagtg ccgtactgac tggtcagcac cgggacgccg
    13621 tcaacatggg cgatgatgcc atcgggtttt tctatcggca ggacgtcgcc gggtttcagt
    13681 tttaaaatct gcgacaggcg tagcgagata tcggcaaagt tggcgaccag ctccagctgt
    13741 gaatgctgca cctggcgcac caggttatcg cgccagttct gatcttcatt acgcgagttt
    13801 tccagcggcg ggttaaccaa caattcccgt agcggctcga tcatgctgaa tggcagacag
    13861 atattaaatt cgccggtcag gttgccaatc tccacatgga acggcgtgtt aaccacaatg
    13921 tcgttcggcg aggtggtgat attggtaaat ttcacctgca tttccgaacg cacgtactca
    13981 acttccagcg gattaatcgc cttccaggcg tcgctatagc cttcaagcgc cagctttaac
    14041 atgcggttga tgacgcgctg ttcggtatgg gtaaactcgc gaccttccac tttggtcggg
    14101 aagcgtccat tgccgccaaa caggttatcc acggcgataa acaccagact cggtgaaaac
    14161 accaccagcc ctgtgccgcg cagcggtttc agatggataa ggttcaggtt ggtcggcacc
    14221 ggcaggttgc gggcaaattc atggtacggc tgaatgcgga tggccccgac ggttatatcc
    14281 gggctacgac gcagcaggtt gaacagcccc atacgaaaat ggcgggcaaa gcgctcatta
    14341 atgatttcca gcgcctgcaa acgttcgcgc acaacccgtc gttgggtatt cggatcgtac
    14401 ggacgaatgt cactttcgcc gctaacactg gctgtcggtt cgtctttgac ttcgctgtca
    14461 ccattcaaca gcgcatcaat ttcagcttga gaaagaatac tatcgcccat gtcgttatcg
    14521 cagaataaaa gcggtataca tcacgtcggt gacatcctgt ttcggttgcc cggcaacaag
    14581 cggggtggaa agcgtggttt taatctcggc aatcaggttt ttcttgcctt cttctgtcgc
    14641 cagtacggca gcatcctgac gcgaaaacag taacagcaaa cggctacgga cttccggcaa
    14701 atactcactc agccgcgagc gggtagcttc atctttgagt cgcagggtta taccgatata
    14761 aagcacgcga tccgcatcgc ccaaattgac cgtaaaggta tccagcgcat agaagaccgg
    14821 tgagggcacg acgcgatgct gcgctttgtc ttcagcggca acctgatgcg aatgccagta
    14881 gctgtaacct gcgctggcac aggccgcgag ggtaatgaat accagaatcg ggagccaaag
    14941 cgatcgcttg cttttcttgc ttatcgcgta atcagtcatg tgttgcggtc ttcctgtgtc
    15001 gctactgctt atcggctaat tatcctgcgt cttgagcgca acaaagcggg gaaaagacgc
    15061 ggattacggt gctacctctg acgttaggcg aaaatatcaa cgccgctgtt gcctgttacg
    15121 cgcccttgta aagagacggg aaccggaagc gtatcgtctt cttcccccgc cagaggttca
    15181 tggtttactg tgcgttggct ttgctgttgt tgggaagcgg cctgctgctg accactaaag
    15241 ctttcgccac tgatgttgct ttgccctaac tgaatgccac tctcggccag ctgcgtacgc
    15301 agtaccggca gcgctgcttc cagggcggcg cgtacgtgct gatgcggcga aaccatctgg
    15361 atttgcgcct ggttatcatc cactttgagg gagatttgca cttcacctaa atcctgcggg
    15421 tgcagacgca actctgcact ttgttgtccc tggcgggtga acagcgaaat atgctggctt
    15481 aatgattgtt gccattcgtg agaacccagc ggtgcactca agacaggcgc ggcgacggtg
    15541 agcagtggct gtgtctggtg tggagtgatt agcgggctgg cggcagcggt caccggtgaa
    15601 ggtgtgctga tgacttccgc tttactctgg gcttctgcta ccagcggtgt taatggctga
    15661 gctggcgtgc cgggggcatc atcaggctgt gctgttgtga gttgcgcaga agtcagtttt
    15721 gtgaagagcg ttggtttctc tgccggtaac acggttgacg gcgcatcagt cactttgggc
    15781 gtattgtcaa aacccggcaa catcgcaaaa agggcgctca ggcttgcggt gaggtcttca
    15841 ttcagatcat ccgctttttc gtcttttgtc gtatttttgt cagccaccgc agccaacgtc
    15901 atcgtatgag cggtggttaa cggtgtcgat gtggattgtt cgacgttgat gacaggcagt
    15961 gtttcatcca cagggatcag taaatccgct tgttgcgcgt cggaaacaat atcgctgatc
    16021 agcagctcgc cttttgtcgt gggcttatct gttgccacca gcacctgggg ggccgctttg
    16081 tcggtagttg tctcgcttgc taatgcttcg ctcaacaacg cgagaaaatc ttgtgcagca
    16141 tcgctggctt tgccgccagg caatgtggtg gtgtcaacgt cggcggtaat caagggcgct
    16201 aagcgaatca ttcaggtttc ctcatggcgg cgcgctgggc gaactcatcc atctttttct
    16261 gatcgaggcg gttttctgca agcagtgccg ccgtggattg ccgttcctgc agtgtctgcc
    16321 aggcctgcaa acgttgtttc ttttctcgcc agctgttcag ggcaatgtca actttctgcg
    16381 tccactgatt aagttgctgg cgatgctgag taatggcttt ttccagcgtc tggataaact
    16441 gctgatagtt gatccagcgg ttgctggtca tcccggcact catatcgctg ttgaggttat
    16501 tgcgatattc attctgataa tcgatcagca ttttgagctg ttcttccgcc tgctgacacc
    16561 cgcgacgcat ttcacccagc aggcgcgcgg catcctctac ctctttttct gccagatctt
    16621 tcagggtcgc cagcgcacca tgttctgcca tctaccgtta tctcctgggt tatgacactg
    16681 tcgggaaaat acgctccagc ccctggagag acgcttccca gtccgcacgt tcaaaaatgc
    16741 cttgttgtaa atagccctcc agctgcggcc acagggcgat ggctttatcg agcatcggat
    16801 cgctgccttt ggcatacgcg ccgacgctaa ccagatcgcg gttacgctga aaactcgaca
    16861 acagctgttt gaaggtgcgc actcgcgcgt aatgttgctc actgatcaac gccgtcattg
    16921 cgcggctgat cgacgcttca atatcgatag ccggatagtg cccggcttcc gccagtcggc
    16981 gagacagcac aatgtgaccg tcgaggatcg cccgtgcgga gtctgcaatg gggtcctgct
    17041 ggtcatcgcc ttcagtaagc acggtataaa acgcggtaat cgagccgccg ccgctaatgc
    17101 catttccggc acgctcgacc agtgccggta atttggcaaa caccgacggt ggataacctt
    17161 tggtggcggg tggttcgcca atcgccagcg caatttcacg ctgggccatc gcgtagcggg
    17221 tgagggagtc cataatcagc aacacatgct gaccacgatc gcgaaaatct tcggcaatgc
    17281 gcgtggcata ggcggcacct tgcattcgca gaagcggaga gacatccgcc ggagcggcaa
    17341 tcaccacgga gcgtgcacgc ccttcagcac cgaggatatt ctcaataaaa tcttttactt
    17401 cgcgcccacg ttcaccaatc aaacccacga caatgacatc ggcgcgggtg taacgtgcca
    17461 tcatccccag cagcacactt ttaccaacgc cggacccggc aaacagcccc atacgctgcc
    17521 cacgcccaac ggtaagcagg gcattgattg ggcgcacgcc ggtgtccagc acatgttcaa
    17581 tcggtgtacg ttgcaacggg ttaaatggcg gggtaatcag cgcaccggtt tccgtcgtat
    17641 cgggggaggg caggccatcg agcggcttac cgctgccgtc cagaacgcga cctaataacg
    17701 ccggaccgag cggcaactgc ttgccgcttt gcagtccttc agccgaaatg tttttggcat
    17761 aaacccgcgc gccgggcagg acaccttcca cttcctccag cggcattaaa aacagccgtt
    17821 gaccgttaaa gccaacgact tcgctttcta cttcgtgcgt ttcgctgccg ttctggcgtt
    17881 caatgacaca ggttgcgccg agcggcaatt gtaatccggt ggcttccagc accagcccgg
    17941 tagcgcgggt taatcgccca tagcgacgta ccgcaggcaa ctgcgccatt ttggcttcaa
    18001 agttatccag cgtggttagc cagcgagtca ggcgcgtggt cattacacca ctcctggtgc
    18061 tgccagacgg cagagttctt gccagcgagt ggcgacactg gcgtcgagat cgccttcatc
    18121 ggcggagact ttacagccgc caggatggag tgtgggatcg ccccgcaagc gccagccatg
    18181 caaacttaag gttgcgccga gcatatcatc cacacgttgc agatcatccg ggtgcacgcg
    18241 cagctgtggt ttaccgctga ataacggttc ttgctgcaac aactgttgga tctgtttgat
    18301 cagtgccgag ttatccactg ttggcgtctg accgatcacc tgacgtgccg cctccagcgc
    18361 catctgcatc aggcgcgaag caatcacact atcaagcgca tcaagggtag tttgaaattc
    18421 gctgaccagt tgctgcatcc gggcatgaat tggcgcttgt tgagacttcg cctctgccag
    18481 accttgctcc agcccctggg ccagtccttc ctgatagccc tgctcatgac cttgctggcg
    18541 accttcggca atccccgcct gataaccttg ctcatgggcc tgcatttgca gttgcgccag
    18601 ttgctgctca aggctaggtt cggcctcttc aatgatggtt tcttccggct cgaccatggg
    18661 cacaaactct gcctgtggtg gcgcgagatc gtccggcgtc caggttttcc acggcagatt
    18721 atcagacata ggtatcctcg ccgctgccaa ttaccatctc gccagtttcg gcaaggcggc
    18781 gcacaatcag cagaatcgct ttctgttcgt tttccacctg cgacagacgc accggaccac
    18841 ggttggcgag atcgtcgcgc agaatatcgg cggcacgctg cgacatattg cgcaggaatt
    18901 tctcgcgcag tggctgctcg gcccctttca gcgcgatcaa cagcgattcg gaatccactt
    18961 cctgcaacag acgctgaatg ctgcgatcgt cgacatccac cagattttcg aacaggaaca
    19021 tctcgtcgat aattttttgc gccagctcgc cgtcgaattc gcgcacggcg gtaataacgg
    19081 cttcttcctg ctgagttttc atcaggttga taatttcggc tgccgttctc acgccgccca
    19141 ttttgctgcg cttgagattc tgaccgtcga gcaagccatt cagtacttcg gtcagttccg
    19201 ccagcgcggc tggctgcacg ccgccaaagg tggcgatacg caacatcacg tcgtggcgca
    19261 gacgttcatc gaacaacgcc agaatatcgg cggcctgcgc gcgcttcaga tgcaccagaa
    19321 tggtggcgat aatttgcgga tgctcatcgc gaatcagatc ggcggcactc tgcggctcca
    19381 taaagttgag cgtttcaata ccgctggcgg tatcgcgagt ttcgagaata tcttccagca
    19441 ggctggcggc acgttcctca cccagagctt tgaccaatac cgaacgcaga taatcgttgg
    19501 cgttgatatt cagtgcggca aactgttcag cttcttgctc aaactccgcc agcacatagg
    19561 ttagctgctt gttggagatc tgcgtgacgt tcgccattgc agcgctcagg gtttgcactt
    19621 cgcgctggga gaggtgcttg aacacctctg ccgcccggtc ttcgccaatg gtcatcagca
    19681 ggatgacgct tttatcggtg cctgtcaggt tactcatgat cgttatttat ccactggcga
    19741 atgaccagcg ccaccacgcg cggatcgtta tcagacattt cacggatacg ctggctcatg
    19801 acttctgccc ccagacgttg gttagcgcgt cgttgttgga gttgttcgtc tttgctcagg
    19861 cggacttcca ccgcatcttc cacttcctca cgggcctgcg cctgttgctg tacagctttc
    19921 atcgcctcgg cgcgacgtgt taactgcggg cgtaccgctt tccgccacag cagccacgcc
    19981 accagcagta ccagcaacca gcgaccggca gccagcaact gatcgataaa cgcttgctgt
    20041 tgccagaacg gcagttctcc gccgctttcg tcactgctat tgaacggcga gttaacgaca
    20101 ttgagcgagt caccgcgttt ttcagaaaag cccatcgctt cgcgggtcag atcttcaatt
    20161 tgcttcatct gttcgttgct gagaggcaat ggtttaccgt ctggcaaggt tttgtaattc
    20221 accactaccg cgactgacag acgttgcaca tcgcccacgt tcattttggt atgacgaatg
    20281 gtgcgatcga cttcgtagtt actggtttca ttccgctgtg tgctacgcgg cccgctattg
    20341 ctggtggtgc tcgcctgctg ctggcggtta ttttgatttg ccggaggcgt gctgattggc
    20401 gcgttattcg caggtgccgg ttgattcgac aacgcccccg gtacgccgcc cggataaccg
    20461 gaaccgcttt gctcgctctc attaagctgg cgtgaacgaa gcgccgcatg agattcatca
    20521 ccgttagggc gatactgttc ttccgtttgt tctttactgg cgaagtccag ttgcgccgtg
    20581 acctgggcgt gaatattacc gttaccaaca ataggcgaca ggattgcttc aatacgccgc
    20641 tgaatacggc cttcgacatc gctggcatat ttcaactgag cgtcattaag atcgcgcccg
    20701 ctggtattgg actgggttaa cagatgtccg ccctgatcca ccagcgtgac gtttcccggc
    20761 ggcagaccag caacggcgct ggaaaccagg tgcacaatgg cgctaatttg cccttcatcg
    20821 agtgcgcggc cgggtaacag atttaccgtc accgatgcag aaggggattt ttgttcacgg
    20881 acgaataaag acggtttcgg cattgccaga tgtacgcgcg ccccttttac cgggccgata
    20941 gtttcgatgg tacgagaaag ctcgccttcc agcgcccgct gataattcac ctgttcgctg
    21001 aactggctga taccaaactt ttcctgatca agcagttcga aaccgaccgc gccgcctttg
    21061 ggcaaacctt gttgtgccag gcgcagacgc agttcgtgaa ctttatctgc cggaacttca
    21121 atagcgccgc tggcttcgct gaagcagtaa ggaatattca tttgcgtcag ttggctgaca
    21181 attgcgccac catcctgatc ggaaagattg ctgaataatg tgcggtagtc gggggctttc
    21241 gcccacagga tcagtgcgac catgaccgcc actgccgcgg aaccggcaac aatcaatgga
    21301 attttcggat tcgcacgcag gcgattaagc cactcaagag attttgtctg ggctgcagtc
    21361 gcattcatcg cgcacctcgt ggctgattat ggacggcgtt attggcaaag tggaacaaga
    21421 ctcactcccg ggtcagcaaa ctcgaaaaat tgacgctgcc attatttgtg ctttcgtttt
    21481 tttctatccg ccaataaacc cgtttttttt gttgctattt agcgcctttg tcttattgac
    21541 ttactggtaa gctttgctac cagaaattat ccgggagatg aaaatgtcag cgatacaggg
    21601 gattgaaggg gttatcagcc agttacaggc tacggcgatg agtgcgcgtg cgcaggaatc
    21661 actgccacaa ccgaccatta gttttgccgg gcagctgcac gccgcgctcg atcgcataag
    21721 cgatacacaa acagccgccc gcacgcaggc agaaaaattc actctcggtg aacccggcgt
    21781 ggcgttaaac gatgtgatga ccgatatgca aaaagcctca gtttctatgc aaatggggat
    21841 tcaggtgcgt aataagctgg tggcggctta tcaggaagtg atgtccatgc aggtgtaggt
    21901 tttgtaacct gttgttaatt acatccgatc tcaccgcgtg gggcatggat ggggcaaact
    21961 cactcaattt ctggtttaaa atggatacct gatcctgaaa gttttctgcc atccacgatc
    22021 cgtaaacgcg ataacatgtt ttgtcttcgc tttgctcata ggaagagtga actcttttgt
    22081 tagcctgggg ttccttctga tcattattgt tcctgccttt atctcttgcc acgccagagc
    22141 cccctggata cacatacacc aggatgaaaa cggtgagttg tgttcaaact gtagtacaat
    22201 tctctccagt atgaacagga aagaatatgc tatgaaccct tatatttatc ttggtggcgc
    22261 aatacttgca gaggtcattg gtacaacctt aatgaagttt tcagaggggt ttacacggtt
    22321 atggccctct gttggcacaa ttatttgcta ttgtgcatca ttttggttat tagctcagac
    22381 actggcttat atacctacag ggattgctta tgctatctgg tcaggagtcg gtatcgttct
    22441 gattagtttg ctttcctggg gattttttgg ccaacgactg gacctgccag ccatcatagg
    22501 catgatgttg atttgtgccg gtgtgttagt cattaacttg ttgtcacgaa gcgcaccgca
    22561 ttaaaaaata atcatccttg agataacagg aatctgattt gtgcatatta tggctaatgt
    22621 tcggggcaac tttataatta tgattgcatt tgcgcgatca ttgcgccttt ttgcataatg
    22681 agatcaatgc acatcaaata aaataacgat aggtcacaac aaagaggttt ttatgaaaaa
    22741 acttatcgtt tcaagtgtat tggcattcat aacattttct gcgcaggccg cagcatttca
    22801 ggtcactagt aatgaaataa aaacaggaga gcaacttaca acgtctcatg tattttctgg
    22861 agttgggtgt gaggggggta atacatcgcc ctcattaacc tggtctggtg ctccagaagg
    22921 caccaaaagc tttgccgtaa ctgtgtatga tccagatgcg cctacaggca gcggatggtg
    22981 gcactggact gttgctaata ttccagcaac ggtaacatat ttgcccgctg atgcaggaag
    23041 acgtgatggt acaaaacttc cgactggtgc tgtccaaggc cgaaatgatt ttggctatgc
    23101 gggatttggt ggagcatgtc ctcccaaagg agataaaccc catcattacc agtttaaaat
    23161 atgggctcta aaaactgata agattcctgt ggattctaat tccagcggag cgttagttgg
    23221 ttatatgctt aatgccaata aaattgcaac cgctgagata atacccgttt atgaggtaaa
    23281 gtaggataag agtatgctgg caaaagatag gactaaccta aagattgaag aaatacggat
    23341 gcataaacat catgagattc acagggttaa gcctcttatg ccagcttcat gtcgtatccg
    23401 tcagggaaaa aaagttatca attgggagac gcatagtcta actgttgata ataatcagat
    23461 aatattattt ccctgtggat atgaatttta tattgcgaat tacccagaag caggtcttta
    23521 tcttgcagaa atgctttatt atcccattga tctaattgaa aagttccaaa atctttatgc
    23581 gataaccgat caaattcgta acacgacagg tttctgctta cctcagaatt ccgagttaat
    23641 atattgttgg gagcaactaa aaacatctat ttcccgaggc ttctcaacgc aaattcagga
    23701 acacttagca atgggcgttc tactttcatt aggagcacat catgttaatt gtttactttt
    23761 atcagatagt aaacaatcat taataagtcg ttgttataac ctaatgctat ccgaacctgg
    23821 aacaaaatgg acagcaaaca aggtagcgag atatctctac atttctgttt ccacattgca
    23881 tcgccgtctg gcaagcgagg gagtaagttt tcaaagtata ttggacgacg tgaggttaaa
    23941 taatgcgttg tctgctatac aaacgacagt aaaacccatc agcgagattg ccagggaaaa
    24001 tggttacaag tgtccttctc gttttactga aaggttccat aatcgtttta agataacacc
    24061 aagagagcta agaaaagcgt ccagagagta aaagtatttt atgaaggagc aacagcatcg
    24121 atttttattt tggaaaataa atacggcata ctcaacctat tggagcctga acaatccggt
    24181 gacttctgcg ctaatcgggg acgtttataa gcacatataa ttgatactat cttcctccat
    24241 cacaatttca gaaatgcacg tgtgattttt ttgcatccgg cgtttggact atgtggagtt
    24301 caagagtggg aaagttacaa gaggttgagg ggataattaa agggatgctt atgacattag
    24361 atatccggat aggtacggat atcgttgtta ttaaatctag tttatacgtt aagatgcagc
    24421 tacagcagaa attgccactg ttaatttttt catcgtgagc ccttttattt gaactattat
    24481 ttaaaaatga tgtcactgca ctacaaatat tcatccaatc aatgagatta tttcaagatg
    24541 taagttttag tttctcattt aaattgtgaa gtagat
//
LOCUS       DAJTYZ010000058        24279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000058 DAJTYZ010000000
VERSION     DAJTYZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24279
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.058"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..201)
                     /locus_tag="OO596_002195"
     CDS             complement(<1..201)
                     /locus_tag="OO596_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6718043.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAV"
     gene            complement(204..1871)
                     /locus_tag="OO596_002196"
     CDS             complement(204..1871)
                     /locus_tag="OO596_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6718044.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(1868..3976)
                     /locus_tag="OO596_002197"
     CDS             complement(1868..3976)
                     /locus_tag="OO596_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6718045.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(3963..4784)
                     /locus_tag="OO596_002198"
     CDS             complement(3963..4784)
                     /locus_tag="OO596_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6718046.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(4943..6772)
                     /gene="glmS"
                     /locus_tag="OO596_002199"
     CDS             complement(4943..6772)
                     /gene="glmS"
                     /locus_tag="OO596_002199"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6718047.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6934..8304)
                     /gene="glmU"
                     /locus_tag="OO596_002200"
     CDS             complement(6934..8304)
                     /gene="glmU"
                     /locus_tag="OO596_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6718048.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8655..9074)
                     /gene="atpC"
                     /locus_tag="OO596_002201"
     CDS             complement(8655..9074)
                     /gene="atpC"
                     /locus_tag="OO596_002201"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6718049.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9095..10477)
                     /gene="atpD"
                     /locus_tag="OO596_002202"
     CDS             complement(9095..10477)
                     /gene="atpD"
                     /locus_tag="OO596_002202"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6718050.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10504..11367)
                     /gene="atpG"
                     /locus_tag="OO596_002203"
     CDS             complement(10504..11367)
                     /gene="atpG"
                     /locus_tag="OO596_002203"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6718051.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11418..12959)
                     /gene="atpA"
                     /locus_tag="OO596_002204"
     CDS             complement(11418..12959)
                     /gene="atpA"
                     /locus_tag="OO596_002204"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6718052.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12972..13505)
                     /gene="atpH"
                     /locus_tag="OO596_002205"
     CDS             complement(12972..13505)
                     /gene="atpH"
                     /locus_tag="OO596_002205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6718053.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13520..13990)
                     /gene="atpF"
                     /locus_tag="OO596_002206"
     CDS             complement(13520..13990)
                     /gene="atpF"
                     /locus_tag="OO596_002206"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6718054.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14052..14291)
                     /gene="atpE"
                     /locus_tag="OO596_002207"
     CDS             complement(14052..14291)
                     /gene="atpE"
                     /locus_tag="OO596_002207"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6718055.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14338..15153)
                     /gene="atpB"
                     /locus_tag="OO596_002208"
     CDS             complement(14338..15153)
                     /gene="atpB"
                     /locus_tag="OO596_002208"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6718056.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15162..15542)
                     /gene="atpI"
                     /locus_tag="OO596_002209"
     CDS             complement(15162..15542)
                     /gene="atpI"
                     /locus_tag="OO596_002209"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6718057.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(16159..16782)
                     /gene="rsmG"
                     /locus_tag="OO596_002210"
     CDS             complement(16159..16782)
                     /gene="rsmG"
                     /locus_tag="OO596_002210"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6718058.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(16846..18735)
                     /gene="mnmG"
                     /locus_tag="OO596_002211"
     CDS             complement(16846..18735)
                     /gene="mnmG"
                     /locus_tag="OO596_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6718059.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSV"
     gene            complement(19114..19557)
                     /gene="mioC"
                     /locus_tag="OO596_002212"
     CDS             complement(19114..19557)
                     /gene="mioC"
                     /locus_tag="OO596_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6718060.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(19647..20105)
                     /gene="asnC"
                     /locus_tag="OO596_002213"
     CDS             complement(19647..20105)
                     /gene="asnC"
                     /locus_tag="OO596_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6718061.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            20257..21249
                     /gene="asnA"
                     /locus_tag="OO596_002214"
     CDS             20257..21249
                     /gene="asnA"
                     /locus_tag="OO596_002214"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6718062.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(21254..22705)
                     /gene="viaA"
                     /locus_tag="OO596_002215"
     CDS             complement(21254..22705)
                     /gene="viaA"
                     /locus_tag="OO596_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6718063.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(22699..24195)
                     /gene="ravA"
                     /locus_tag="OO596_002216"
     CDS             complement(22699..24195)
                     /gene="ravA"
                     /locus_tag="OO596_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6718064.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         6007 a         6312 c         5773 g         6187 t
ORIGIN      
        1 aacagcgtaa cgtcctgttt gatgtagatg tcttgctatc acacctaaat gcgagggcag
       61 acctaaggta gcgaccacct tgcggttgcc atacacctca tccaacagct gcttaggact
      121 aacaatccct tgataaacgc ctgcccgtgc aatagtgcta taaatcagct cattcgagta
      181 cggaacagga aagtttctca tggctaacct gcttgtttaa ataagctcgc catatccact
      241 attacccctt tcccttttaa ccgttcatgc atggtttttt caggttggcg ttgtgaatag
      301 atataacgta aatccgtatc aggcaagcta tcccagtcgc tgggcttgac tacctttata
      361 gccgaaacct ttttactctt ggagggcttt tctagttctg acactaccgt ttcgccttcc
      421 atcaaccact gcaaaacaag aggcagtaac ttttgtcttg tcatcgttgg attctggcta
      481 aacgcttttt taatagtggg aattaacagg cttgaatcgt aatcctcttt cagcatcaga
      541 tataaatgac gctgatcttc ggtatctaac tcttgaaggg ctttttcttc tggtgtttgt
      601 tcttgtatcg ctgcgatatc tagctgaagt tggattaacc gtttatcaat ctcgggaacg
      661 actagatcag aataacgagc aatgcgttct gggatacccg agcgtaatgc ctctagcatg
      721 gggtgcacag gctttaactc atcttgatac acttgccgca ataaaccagc ggtaatacgc
      781 tcattgccta aagctagcgc acggagctga gcgagtacaa aaagttttac tacaatgtcc
      841 atcactcctt ggcttagctc ataccacaca tcacggacct catccgataa cagcgcatct
      901 ttgcgttgta aaagctgtaa ttgccaaaga ttatccgtaa aagcgatcca ctcttgattg
      961 ggctttccac gttgcgtttg ttgtatagga tcccagaata tagctccaaa ccctgcccct
     1021 ctacgtgcag accgcaaatc agcctcaaaa atctctcgtg ctttaggggt accaatcaac
     1081 atcactggta cgccaataat attcaccatc gtcacaaaaa agttcagcat ctcttgagat
     1141 ccacccgaac gagagcggct taaatgctga atttcatcaa taaccaacaa ccctaaagca
     1201 tgtgcattgg ctatttgcga catcaaagcc aacatggttt ctataccatg acgttttaag
     1261 ccataacgac gctcatagtt cgagcccaag gctcgatcca acgctctgaa aaaattcaag
     1321 cagatttctt ttagcgaacc attatgcgag cagtctattt tcaaatacac cacctgctct
     1381 acattgagtt cacgatggta aatcacctga ggatacgtgg ctagaatacg atgaagagag
     1441 gtcgtcttcc cactaccaga acaaccaatt aacaataagc tttgtgccgt agatcgtgcc
     1501 tcctcaaagc gaaatgtctc caactctccc gtttgaacac gctcataacc attttgtaaa
     1561 tgcttttgta aatctcctgt tttaggattt ctgcctacgt agccacctcg aatcatgacc
     1621 gaaatacgct cacttagtag caaatgcgta cctaatggct gaaaatagtc atctggaata
     1681 cgacaaatgg tatgagctct gataacacgg gacttttgca agtcagagga agtaagctgt
     1741 aaagaggatt tcagtgatgc agcactattc actgactctt gtaatggtgg taaggcctcg
     1801 ataaaaggat tatcacgata agcctctacc cccgtatcac gataaactgc ttgaatccgg
     1861 gtagcactca tgactcatcc ttttctggtg gatcctgaaa taattcaggc acgtatgtgg
     1921 gtaggctgta atcttcttga tcatccgctt ccactgcgtt gaaaggaata actttagcct
     1981 catcacctga agagcttggc ttcaaatgct ccgcacgttt tttacgctcc gaggtcacgg
     2041 cttctttttt attagtttta atctgcttaa tgcgtgttga tttgggctca gtagtactgg
     2101 gcgttaactt attcgctttc tgaatggttt gctgaataaa cgcctcaagc tccctgcgtt
     2161 tagttaactc atcctgcttc gcattggctt tattgtgttt ttcttgtgct tgtatatccc
     2221 aaacctccca aaatgagaga cctttaaact gccgactacg ttccgtcaga ttacagcgcc
     2281 aaaatacacg gctgccaact tgcggaaaca aataaatcgt atcaaccagc actgggtcat
     2341 aagccgcttc taaatgttga ggtctagcta tatcagtgct ccgctgcaac caaccctcac
     2401 gcagaatctc tgaccccgag taatacaaac cccacaaatt aacgccaaat gaagaaatag
     2461 agacctttcg gcgaggcagt aacgctactc gcaactgctc ttgctccaca gcccttaaac
     2521 tacctgtacg atgctgcata ccccattgcc atagctggac aggaatagac ggtaaatctg
     2581 taggaaaatc agcatctcga tcgtatttat ccatcaccag atggttattt ctgaataaga
     2641 tcgtacgcaa aataatttgt gtgaactcaa ataccgacag agatgcatct aacctatagt
     2701 ctgtttcacc atggcttttg atccgactgc cctctacaat gccaggtgca aaggacttaa
     2761 actcggcttg tagtgttcta aaagtgcttt ccactatgcc tttagcatcg ccacgtctag
     2821 gtggagcact ttccactcgc acattaaaac tagaaactaa ggcttcgacc tgatgactca
     2881 ttaattcgcc acggtccgct agcaacacat ctggcaaacc tacacacggc cagtctgagc
     2941 tactaatctc aatatcatgc tgggcacaaa tggccgtttt gtcagagcaa gcatttacaa
     3001 aagcctgcat cgccaccaca taagacggat tttcaaagcc gatataaaag cccgtgatca
     3061 tccgactaaa cacatcaatc acaatgtaaa gcgttggtct tcctatgatt ttttggcgat
     3121 catgatgatc cactaaataa atatcagcaa tcgtggcatc aatctcataa cgactcccag
     3181 ggcctaacgc ctgagaagtg gctgtactac ttaagggtcg tacgtctttt ttatataccc
     3241 ctgctttaac tctagacttt aagcgctgag ctttagggta ttctcgatca taaaaataac
     3301 gaaactgacg tagtgttggg taatcctctt ggggaatgcg aggaaaatac tgagcaaaca
     3361 agtccacaaa tcgtctatag gcaacggtgg tctttgtacc tttttgattt aacaggtgct
     3421 tttctatggt caacctaaaa aggcgttcaa tctcgggcgt taccttggtt ccttcaccct
     3481 taccatattc tctggctcgg ccaatctttg ctgttcctgt cgctgaacgt ctttcccctg
     3541 gtgcaccgct gtttttgtag tcaggaatta atgcattagg cgtttgacca cgctgccagt
     3601 aacggcgtaa caacttataa actgtagcct tagtaacctt atgttcttgg accacatgct
     3661 caacgagttc gcttctgact ttagggtcga aacgatcctt actattaata ataggaagaa
     3721 tttttcgata atcctcctcg cgcttctgga agctgacaga atctacagac ggctcttcta
     3781 aatcaagatg tacataaggg tcatcagttc taactaagcg gccttcatct aaatactgca
     3841 ttagcaacaa ctcagcccta gcttgtggaa ctcctttatc agcgcttatt tgcatccaga
     3901 caacttggcc atcctctata gccaaaatgc gatacggatc attatcaaat agcacaacct
     3961 cattaatttg ccacatagcg caactcctcc atattcacta cttggctaat acagagatct
     4021 gcacacttat ttgccctgaa agacttataa atattgaact taataaaacc atttgcggtt
     4081 aaggctcgta tctcactcaa tgttttgcct aactccaaat cataagcaat atcaacctgc
     4141 ttacagacat tgataatgtt ttcatctcct ttttcttgca ggatatgggc caatggggat
     4201 agttgtgcta aaagctccgc agaaacttct tctgttttca ctgaataaag ccattcaata
     4261 ttttctttta ctacgggatt tatttcttta tcagtaaaaa tgaaccaagg aatttgcttt
     4321 tgctgccaat agcgacgctc tagttctagt ttttctaagg tacgctcgtc ttgtaaggct
     4381 gctgcaggtt tgacttgaat agcaaactgc tcaaaaggac catctttgca gtccactaaa
     4441 aaatcagtag acataacctg atctacacca cgaataacag gatgcttaat accactatct
     4501 attgcaatct gcctggtatc actaggtaat aaggggaact gctcgcgtat atctagcacg
     4561 ctgctctccc actcaagact gagaaaaaca gcaagctcta agtcagatag caaatgatgg
     4621 actcgtcccg tcttatgaga ataaatacgg tgggaacgac ctgaagaagg aacttcttgt
     4681 actgttagcc atggaatata gtctttacca tgcccttggc cacgcccctc tttaatacga
     4741 cgggcaattt gcacttcaga aaatgaagag tttgctttag ccataacaaa agtccagtat
     4801 gctttttcac agcataactg gactgatttc agtttacaac tattctgtct agtttaagac
     4861 tttattgtca tagtttagat ctattttgtt cagtttaaga ctttattgtc cgcccacagc
     4921 ccttttttat ctatgatagt gattactcaa ccgtaaccga ttttgccagg ttacgcggct
     4981 ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc cagcagctgc agcggaacgg
     5041 tgtagaagat cggtgcaatc acctcttcca catgcggcat ctcgatgatg tgcatgttat
     5101 cgctacttac aaaacccgca tcctgatcgg cgaagacata caattggccg ccacgcgcgc
     5161 gaacttcttc aatgttggat ttcagttttt ccagcaattc gttgttcggt gcaacgacga
     5221 taaccggcat atcggcatca ataagcgcca gcggaccgtg tttcagttca ccagcagcgt
     5281 aggcttcagc gtgaatgtag gagatctctt tcagcttcaa tgcgccttcc agtgcgattg
     5341 ggtactgatc gccacggccc aggaacagtg cgtgatgttt gtcagagaaa tcttctgcca
     5401 gagcttcaat gcgtttgtcc tgagatagca tctgctcaat acggctcggc agcgcctgca
     5461 gaccatgcac aatgtcatgt tcaatggagg catccagacc tttcaggcga gacagcttcg
     5521 ccaccagcat caacagcaca gttaactgag tggtgaatgc tttagtggac gccacgccga
     5581 tttccgtacc cgcgttggtc atcagcgcca gatcggattc gcgcaccaga gaagaacccg
     5641 gaacgttaca gattgccagc gaaccaaggt aacccagctc tttcgacaga cgcaggcccg
     5701 ccagggtatc cgcggtttcg ccagactgtg acaaggtgat catcaggctg ttacgacgca
     5761 cggcagattt acgatagcgg aattcagagg cgatttcgac gtcgcacgga atacctgcca
     5821 gcgattcaaa ccagtagcgg gaaaccatac cggagttata agaagtacca caggcgagga
     5881 tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt cggtcccagc tcgcttaaat
     5941 caacctgacc gtggctgatg cgcccggtaa gggtgttttt tatcgcgttc ggctgttcgt
     6001 agatctcttt ctgcatgtag tgacggtaaa tacctttatc gcccgcgtca tattgcagat
     6061 tggattcgat atcctgacgt tttacttccg cgccagtttt atcgaagatg tttaccgaac
     6121 ggcgagtgat ttccgcaata tcgccctctt caaggaagat aaagcgacgg gtcaccggca
     6181 acagcgccag ctggtcagaa gcgataaagt tttcgcccat acccaggcca atcaccagcg
     6241 gactaccaga acgtgccgcc agcagggtat ccgggtgacg ggagtccatg atcactgtac
     6301 cgtacgcacc gcgcagctgc gggatagcac gcagaacagc ctcacgcaga gtcccgcctt
     6361 gtttcagctc ccagttcacc agatgggcaa tcacttcggt gtcggtttca gaaacgaagg
     6421 tatagccacg cgcttttaac tcttcacgca gcggttcatg gttttcgatg atgccgttat
     6481 gcaccaccac aatgtgttca gaaacatgcg gatgcgcgtt agcttctgaa ggttcaccgt
     6541 gggtcgccca gcgagtgtga gcaataccgg tgccgccatc cagaggatgt tcttccgctg
     6601 cctgagccag catctggact ttaccgaggc gacgcaggcg ggtcatatga ccttccgcat
     6661 caacaacggc cagaccggca gagtcatatc cgcggtattc cagacgacgt aaaccttcaa
     6721 gaaggatttc tgctacatca cgttgcgcga tcgcgccaac aattccacac atagtttttg
     6781 attccgattt atatcgttgt cggtcaacct gtatgcccgt tttttcgggc gccccgagcc
     6841 ttgtagagag tggggttatt tttatagtta ctgcttgtgg gagggagatt atgttatctc
     6901 ctcatcccag atgaccggat tacccggcca gaattacttt ttctttaccg gacgacgcca
     6961 gccttctttc tgagtctgcg gcacacggct gatagctaat gcattttcgc cgacattacg
     7021 cgtcacagtt gtacccgcag caatggtcgc gcctttgcct actgttaccg gggccaccag
     7081 ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg gtcttaaatt tattcgcacc
     7141 atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg ttatcgccaa tttccgcatc
     7201 gcccaggtaa gtcagatgcc cgactttcga gcctttaccc agacgcgctt ttttcatctc
     7261 aacgaagtta ccaacgtgtg caccttccag caactcagca ccaggacgca aacgagcaaa
     7321 cgggccaata gtacaggccg ctgccagatt cgcgtcttcc acaacggtat acggactgat
     7381 ttcgcaatca tcgccaatca cgctgttttt aatcacgcaa ccggtgccga ttttcacgcg
     7441 atgaccgaga gtcacgttgc cctcgatgat aacgttagta tcaatttcaa catcgcgccc
     7501 gtgagtaagc gtaccgcgca gatcaaaacg cgccggatcg cgcagcataa cgcctgctaa
     7561 cagcagtttt tcagcctgtt cggactggta aacgcgctcc agacgggaga gttgcaggcg
     7621 gttattcaca ccttcaactt cgcttaaacg ttgcggatga acggcgacga tttcacgccc
     7681 ttcctgatac gccagcgcaa taatgtcggt gatgtagtat tcgccctgag cattattgtt
     7741 ggtcagcttc gccagccagc gtttcatatc tgcgccgtta gcgatcagaa tgccggtgtt
     7801 gatctcctga atctgacgct gctcgtcggt ggcatctttg tgctcaacaa tgccggtaac
     7861 tttgccattt tcacgggtga tacgtccata accggtcgga tcatccagtt tcaccgtcag
     7921 cagaccaatg ccaccctgcg gtttagcatc acgcaggcgc tggagtgttt cgacagagat
     7981 cagcggcacg tcgccgtaga gcattaaaat gtcttcatca tcggcaaaga aaggtgcggc
     8041 ctgctgcatt gcatgacccg tacccagctg ctctgcctga agcacccagt taaggttgtc
     8101 gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg tacaccaagt gaacgtgcgc
     8161 tgcgcctaat tcattcgcag catcaatgac atgctgaacc atcgctttcc cggcaagggt
     8221 atgcagcact ttcggaagat cggaatacat gcgcgtgcct ttgcctgcgg caaggatcac
     8281 tacgctcata gcattattca acatacgcgt cctgactgta atttgagaac gaatttaaac
     8341 cgcttcacct tgaaaaaact acattttttt catcgtgaaa tggacagagg ataaattgtt
     8401 caataacgga ttatccccgc tgacgaaagt gccattttcg accatcgtca cgccttttgt
     8461 ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg tggatgtttt tgctcttatt
     8521 tcgatcaatg aataaacggg aaaacagggg tgttttatat cagcaggatc tatgtgaacg
     8581 ctcttcagga cggatcacgc gtaaaaaaaa gccagcctgt ttccagactg gcttttgtgc
     8641 ttttcaagcc ggtgttacat cgcttttttg gtcaactcga taacgcgcag ctgcgcgatc
     8701 gctttggcca gttccgcaga cgcctgagcg taatctacgt cgccgtgaga gctgctaatg
     8761 tgctcttcag ccttacgttt cgcttccatg gctcgcgctt cgtcgagatc ctggccgcga
     8821 attgcggtgt cggccagaac ggtcacgttg ccaggctgca cttcaagaat gccgccagac
     8881 agatagataa actcttcgtg accgtgctgt ttcacgatgc gaatcatacc aggcttaatg
     8941 gcggtgagca gcggtgcgtg gccagggtag atccccagtt caccttcgct acccgttacc
     9001 tggattttct cgaccagacc agagaacatt tgttgctctg cgctgacgac gtccaggtgg
     9061 taagtcattg ccatatcacc ctccgattaa ggcgttaaag ttttttggct ttttccacag
     9121 cttcttcgat ggaaccgacc atgtagaacg cctgctccgg caggtgatcg tattcgcctt
     9181 ccatgatgcc tttaaagcca cggatggtgt ctttcaggga gacgtattta cccggagaac
     9241 cggtgaatac ttctgccacg aagaacggct gggacaggaa gcgctggatc ttacgagcac
     9301 gcgctaccac cagtttgtct tcttcagaca gttcatccat acccaagatg gcgatgatgt
     9361 ctttcagttc ctgataacgt tgcaggatgg actgaacgcc acgcgcggtg tcgtagtgtt
     9421 cctgaccaac caccagcggg tccagctgac ggctggtgga gtccagcggg tcaacggccg
     9481 ggtagatacc cagagacgcg atctgacggc tcagtaccac ggttgcgtca aggtgcgcaa
     9541 aggtggttgc cggagacggg tcagtcaagt catccgcagg tacgtatacc gcctgtacgg
     9601 aggtgataga accagttttg gtggaggtga tacgttcctg cagaacgccc atctcttccg
     9661 ccagggtcgg ctgataacct accgctgaag gcatacggcc cagcagtgcg gatacttccg
     9721 taccggccag ggtgtaacga tagatgttgt caacgaacag cagaacgtca cgaccttcgt
     9781 cacggaattt ctcagccatg gtcagaccgg tcagagcaac gcgcagacgg tttcccggcg
     9841 gctcgttcat ctggccatac accagggata ctttgtcgat aacgttggag tcggtcattt
     9901 cgtggtagaa gtcgttaccc tcacgagtac gttcacctac gcccgcaaac acagagtaac
     9961 cggagtgctc gatcgcgatg ttacgaatga gctccatcat gtttacggtt ttacctacac
    10021 ccgcaccacc gaacagacca actttaccgc ccttagcgaa cggacacatc aggtcgataa
    10081 ctttgatacc ggtttccagc agttcctgag agtttgacag ctcttcgtag gaaggtgctg
    10141 cgcggtgaat cgcccaacgc tcttcttcac cgatctcgcc tttcatgtcg accggttcac
    10201 ccagtacgtt catgatacgg cccagagtcg ctttacctac cgggacttcg atcgggtgtt
    10261 cgaggtcttt tacatccaga ccgcgacgca gaccgtcgga ggaacccatt gcgatggtac
    10321 gcacgatacc gccgccgagc tgctgctgaa cttccagcac cagacgctca ttaccatttt
    10381 gcacctcaag agcatcgtac acgcgcggta cggcatcctg agggaattcg acgtcaacta
    10441 cggcgccgat tacctggaca atctttccag tagccatctt aaatcctcta cgaaataacc
    10501 tgtttaaacc gcggcggccc ccgagacgat ctcggtgagt tcctgagtaa tgctggcctg
    10561 acgagctttg ttgtatacca actgcagctc tttaatcagg ctgccgccat tgtcggtcgc
    10621 ggctttcatc gccaccatac gggcggcctg ctcgctggcc aggttttcaa ccacgccctg
    10681 ataaacctga gattcgacat aacgacgcag cagggtatcc agcaacgcct tcggatcggg
    10741 ttcgtacagg taatcccagg atttatgttt cagatcatca tcatctgatg ccggtaacgg
    10801 cagcagctgg ctgatggtcg gaacctgaga catggtgtta ataaatttgt tgctgacaat
    10861 gtaaagcttg tccagacggc cttcgtcgta agcctgcaac atcactttta ccggaccgat
    10921 cagttcggac agggaagggt tatcccccat gccggtgacc tgggcaacaa cattgccgcc
    10981 cacggagttg aagaacgaca cgcctttcga gccgatcatt gcgaggtcgc attgaacgct
    11041 tttgtcggtc caggtcttca tttccgccag tagttttttg aacaggttaa tgttcaaacc
    11101 accgcacaaa ccacggtcgg tcgacaccac caggtagccc acgcgtttaa cgtcgcggtc
    11161 ttccaggtaa gggtgcttat attccagatt accgtgtgca aggtgaccaa tcactttgcg
    11221 catggtttct gcataaggac ggctggccgc catgcgatcc tgcgatttac gcattttgga
    11281 agcggcgacc atctccatcg ctttagtgat cttttgcgtg ttctggacgc ttgcgatctt
    11341 actacgtatc tcttttgcgc cggccatgag cttctcctca atgccttgcg gcctgcccta
    11401 aggcaagccg ccagacgtta ccaggattgg gttgctttga aggaatcgag gatgcctttc
    11461 agcttgcctt cgatttcgtc gttgtagcca ccggtctggt tgatctcttg catcaacgga
    11521 gcgtgatcac ggtcgacgta agccagcaga gcggcttcga agctgccaat tttcgacagt
    11581 tcaacatccg ccaggtaacc acgttctgct gcgaacagaa ccagagactg ctgcgcaacg
    11641 gacatcggcg catactgttt ctgtttcagc agttcggtca ctttctgacc gtggtcaagc
    11701 tgcttacgtg ttgcatcgtc aaggtcggat gcaaactgag agaacgctgc cagttcacga
    11761 tactgtgcca gagcggtacg gataccaccg gacagttttt tcatgatctt ggtctgtgct
    11821 gcaccaccaa cacgggatac ggaaataccc gggttaaccg caggacgaat accggcgttg
    11881 aacaggttgg tttccaggaa gatctgacca tcggtaatgg agattacgtt ggtcggaacg
    11941 aacgcagaaa cgtcacccgc ctgagtttcg ataatcggca gtgcggtcag agaaccggtt
    12001 ttccctttca cttcaccttt ggtgaaggct tcaacgtatt cggcgttaac acgtgcagca
    12061 cgctccagca gacgagagtg gaggtagaaa acgtcgcccg ggaatgcttc acgtcctggc
    12121 ggacgacgga gcagcaggga gatctgacgg taagcaacag cctgtttaga caggtcatcg
    12181 taaatgatca gcgcatcttc accgcggtca cggaagtatt cgcccattgc gcaaccggca
    12241 tacggtgcca ggtattgcag tgcagcggat tcagacgcgg ttgctaccac aacgatagtg
    12301 ttagccagtg cgccgtgctc ttccagttta cgtaccacgt tagaaatggt ggacgctttc
    12361 tggccgatag cgacatagat acatttgata ccggaatcgc gctggttgat gatggcatcg
    12421 atagccagtg cggttttacc tgtctgacgg tcaccgatga tcaattcacg ctgaccacga
    12481 ccgattggga tcatggagtc aacggcttta taaccggtct gtaccggctg atctacggac
    12541 tgacgttcga taacgcccgg agcgattgct tctacagcag agaagccgtc gtgatccagc
    12601 ggacctttac cgtcgattgg tgcacccaga gtgttaacca cacggcccag caggccacgg
    12661 ccaaccggaa cttccaggat acggccagta cacttaactt tcatgccttc ggcaaggtca
    12721 gcgtacggac ccataacaac cgcacctaca gagtcgcgct caaggttcag tgcgatagcg
    12781 taacggttac ccggcaggga gatcatttca ccctgcatac aatcggccag gccgtgaatg
    12841 cggataacac cgtcacttac agaaacaata gtaccttcgt tgtgagcttc actcacaaca
    12901 ttgaactgag caatgcgctg cttgatcagt tcgctgattt cggtggaatt cagttgcatg
    12961 ctccagtccc cttaagactg caagacgtct gcaaggcgct caagacgacc gcgtacgctg
    13021 ccatcaatga ccatatcacc cgctcggatg ataacgcctg ccattacaga cttatcgatt
    13081 ttgcaattca gcttaacttt gcgtgacaga cgtttttcca tcgcagcaga aattttcgcg
    13141 agctgttgtt cactcagtgc ggcagcggaa atgacgtcta cctcagcggt agcctcactc
    13201 acggcacgaa ggtgaataaa ctgctccaga acatccggga gcgcgttaag acgaccattt
    13261 tcagccatta cccgaatcag gttctgaccg ttttcgtcca gttgctcacc acaaactgcg
    13321 ataaacgact cggcgagcgt ttctggcgca agcgcgccag agagaagctc tgccatttgt
    13381 tcgtttttgg ttacctcggc ggcaaacgcc agcatgtcct gccagcgttc tacactttgg
    13441 tgttcgacgg caaagtcaaa agctgctttg gcgtaggggc gagctaccgt aataaattca
    13501 gacatcagcc cctccctcct tacagttcag cgacaagttt atccacgatg tcgctgttag
    13561 cagcttcatc cacggaacgt tcgatgatct tctcggcgcc agcaacagcc aggatagcaa
    13621 cttgcttacg cagctcttca cgggcacgtt tacgctcggc ttcaatttct gcctgcgcct
    13681 gggccacgat tttagtacgt tcctgttctg cctcagcttt cgcttcgtcc agaatctgcg
    13741 agcggcgttt gttcgcctgc tcgatgatta cctgggcttc cgctttcgct tttttcagct
    13801 ggtcggtcgc gctggccttt gcaaggtcaa ggtccttatg tgctcgttct gcggaagcaa
    13861 ggccgtcagc aatttctttt tgacgttttt cgatggctgc cattaatggc ggccatacgt
    13921 acttcatgca gaacagaacg aacaggacaa acgcgatggc ctggccgagg attgttgcgt
    13981 taagattcac agcacaatgc ctctatttag ttaacgttct gatattgctc tttaaataaa
    14041 agcaacgctt actacgcgac agcgaacatc acgtacagac ccagacctac agcgatcatc
    14101 gggatagcat ccaccagacc cataacgata aagaactgag tacgcagcag aggaatcaga
    14161 tcaggttgac gcgctgcgcc ttccaggaat ttacccccga ggatgccgat accgatcgca
    14221 gcaccgattg ccgccagacc catcatcaca gcggcagcca tgtacagcag atccatattc
    14281 aggttttcca tgacagtctc cagtttgttt cagttaaaac gtagtagtgt tggtaaatta
    14341 atgttcttca gacgccatcg acagatagac gatcgtcaga accatgaaga tgaaggcttg
    14401 cagcgtaatg atcaggatgt ggaaaatggc ccacggcaca ttcaggatcc actgtgacca
    14461 ccacggcaac agaccagcaa tcagaatgaa aatcagctca ccggcataca tgttaccgaa
    14521 cagtcgcaaa ccgagtgaaa ctggtttgga cagcaggctt accccttcaa ggattaagtt
    14581 gacaggaatg aacgcccagt gattgaacgg ctgcagcgtc aactctttcg tgaagccgcc
    14641 gatgcctttc attttgatgc tgtagaacag aatcaggata aatacgccca gtgccataga
    14701 cagcgttacg ttcacgtccg cagacggaac cacacgcagt gcaggcagac ccagtacatg
    14761 ttcagcaatg tacggcagca ggtcgatagg cagtaaatcc atcaggttca tcaggaatac
    14821 ccagacgaag atcgtcaggg ccagcggagc aatcagcttg cttttgccat ggtacatgtc
    14881 tttcacgcta ccattaacaa agccgatcac cagctcaatc gcggtctgaa acttacctgg
    14941 cacaccgctg gtcgcctttt tggctacgct acggaataaa accaggaaca acagacccag
    15001 caccaccgag aagaacatgg agtcaatatt gattgtccag aaggtggctg gggggttttg
    15061 tggatccacc agcgagaatg tacgcaggtc cagctgaagg ttattcaggt ggtgtcctat
    15121 gtaatcctgc ggcgtcatat tttctgaagc catgatgcct tttacccttt gttgttaatt
    15181 acagccggtg ccagtatctg aaccaccagc accaaaaccc acgtaacgat cagcggcaag
    15241 aataccgcct ttaaaaccgc caacgccacc accagtaaca ccaacatcgc cagaactttg
    15301 aaagcttcgc caaatgcgaa tgtccaggcc acccggcctt tcgctggtgt atacgcctgg
    15361 tgacgccagg caaatatcat aaacaaaacg ttaggcagaa agactgccag gcccccgctt
    15421 attgcagaga cgccccagaa ggggtctttg aggctgaaca gcaatccact tgctatcacc
    15481 accagtaact gaacgagcag aagcttccga gcaacgtttc gactcacgag cgacacagac
    15541 atcacgtttt tcactcctgc tcccttcgag gtatgccgcg tgtcgtataa aactttcttt
    15601 aaggcttaga gtcaagcatc aaaaagcggt caaattatac ggtgcgcccc cgtgatttca
    15661 aacaataagt agccaaaagg tgaataaatg tttaaatatt tttccagtgc atacaattgc
    15721 gacttttccg ctaaccctgt tcgatcatga aaaactgtaa ataacgcgta aacactagtg
    15781 ataaagcgtg cttcagatca catattgcgc atgttcgcgc acagtatatt tatttacttg
    15841 gcaaatgatg cctttgcaag tttatgatat ttcagtctaa aaacagatac tgttttaata
    15901 aatgacattt acacaacaaa aaccacccat tgacattttt aataatgttt taacagccaa
    15961 tgatggttct tagcgccgat ttttagcaga ctgatatttt cactaatgac ttattttctg
    16021 cttaccaaaa aaagccacgt tatcttgttg atacaaaaga gtgaacgtgg cgttaaatgt
    16081 aaccagttat atcagtagaa aacttggttg ttgttaacag tctaaccggt caatttttta
    16141 tgattttttt gataaaaatt aaattttatt tgctttaatc accaccagat gacgttcgcc
    16201 atccagagct ggaacctgaa gtttaaccac tgattcgacc tgatattctt cgggcaacaa
    16261 agcgatttca tcttccggca tttgcccttt cagcgcgtag aaacggcctt gctcaccagg
    16321 aagatggtgg caccagctca ccatatcgtt cagagaggca aaagcgcggc taattacgcc
    16381 atcaaatggc ggctctgaag gaaactcttc taccctgctc tgtactggct caatattctc
    16441 cagtttaagc tcatgttgca cctgacgaag gaaacgcacg cgtttaccaa ggctatccaa
    16501 cagagtgaaa tgggcttcag gacgcacgat agagagtgga atgcctggca gtcctggtcc
    16561 ggtgccgaca tcgataaacc gttcaccttg cagatacggt gccaccacaa tgctatcgag
    16621 aatatggcgt accagcatct cattaggatc gcggaccgaa gtcaggttgt acgctttgtt
    16681 ccatttatgc agcatattca cgtaggcaat aagctggttt ttctggtgat cggtaagcga
    16741 aatacctgcg tctttcagca gtaaggagag tttgttgagc acggtgatta cctgttcttg
    16801 atgcgttgcc tggtaagcgg gtgcttacca ggcattttta atgcgttata cgctacgacg
    16861 cagcataccc tgttttttca gccacaccag cagaatggag atagccgcag gcgtgacgcc
    16921 agaaatacgc gaagcctggc cgatagaggc cggtttgtga tcgttaagtt tggcgatcac
    16981 ttcgttagaa agaccggata cctggcggta atccagtgtc gcgggtagca gggtgttctc
    17041 gttacgcagc tgcttttcga tctcatcttg ctggcgcgcg atataacctt cgtatttaac
    17101 ctgaatctca acctgttccg ccgcctgttc gtctgtcaac gcaggggcaa acggcgtcag
    17161 cgtggttaat ttttcataag tcatttccgg acgacgcagc agatcttcac cactggcttc
    17221 acgggaaagc ggcgcagtca ggtgagcatt cacttcggct gcagcttccg ccgacggggt
    17281 tacccaggtc gatttcagac gctgacgctc acgctcgata ttctcaagtt tctcgttaaa
    17341 gcgcgcccaa cgttcgtcat ccaccaggcc cagttcacga ccgatttcag tcaaacgcag
    17401 atccgcatta tcttcgcgca gcatcagacg atattctgcg cgcgaagtaa acatacgata
    17461 cggttctttg gttcctaaag tgcacaggtc atcaaccagt acgccgagat acgcctgaga
    17521 acgcgccgga gcccaacctt ctttgtcagc agacagacgg gcagcgttaa gaccgaccag
    17581 caaaccttgc gcagcggctt cttcgtaacc ggtagtgccg ttaatctgac cagcaaagaa
    17641 cagcccctgg ataaacttgc tctccagcgt cggtttcagg tcgcgtggat cgaagaagtc
    17701 atactcaatg gcataacccg gacgcacgat cttcgcgttt tccatcccct gcatggagcg
    17761 gacgatttgc atctgcacat cgaacggcag gctggtggag ataccgttcg gataaatttc
    17821 gttagaggtc agcccttccg gttcaaggaa gatctgatgc tgatttctgt cggcgaagcg
    17881 catgactttg tcttcgatcg atgggcagta gcgtgggccg acaccttcga tcacccctgc
    17941 gtacattggg ctacgatcga ggttactgcg gatcacatca tgggttttct cgttggtatg
    18001 agtgatataa cacggcacct gctggggatg ctgggacgca ttgcccataa acgagaatac
    18061 cggcattggg ttatcgccat gctgttgcgc cagcacacta aagtcgatag ttcgagcatc
    18121 aatacgcggt ggtgtcccgg ttttcagacg accaacgcgc agcggcagtt cacgcaaacg
    18181 gcgagaaagc ggaatggacg gcggatcacc agcacgacca ccgctgtaat tatccagacc
    18241 gatatgaatt ttaccgtcga ggaacgtccc aacggtgagc acgacggctt tggcacggaa
    18301 cttcagtccc atttgggtaa cagcaccgac cacgcgatcg ttttcgacaa taagatcttc
    18361 aaccgcctgc tggaagatca tcaggttcgg ttggttctcc agcgccgtac gtaccgcctg
    18421 acggtagagc acacgatccg cctgagctcg ggtagcgcga accgccggtc ctttgcttgc
    18481 gtttagtatc ctaaactgga tacccgcttg atcgatcgct ttcgccatca gaccgccgag
    18541 tgcatccact tcttttacca gatgtccctt cccaataccg ccgatcgccg ggttgcagct
    18601 catctgcccc agagtgtcga tattgtgtgt caaaagcaga gtctgttgac ccatacgcgc
    18661 cgcggccatc gcggcctcgg tgcctgcatg acccccgcca atgatgatga cgtcaaaagg
    18721 atccggataa aacatggtga ttgcctcgca taacgcggta tgaaaatgga ttgaagcccg
    18781 ggccgtggat tctactcaac tttgtcggct tgagaaagac ctgggatcct gggtattaaa
    18841 aagaagatct atttatttag agatctgttc tattgtgatc tcttattagg atcgcactac
    18901 cctgtggata acaaggatcc ggcttttaag atcaacaacc tggaaaggat cattaactgt
    18961 gaatgatcgg tgatcctgga ccgtataagc tgggatcaga atgaggggtt atacacaact
    19021 caaaaactga acaaccgttg ttccttggat aactaccggt tgatccaagc ttcctgacag
    19081 agttatccac agtagatcgc acgatctgta tacttatttg agtaaattaa tccacgatcc
    19141 cagccattct tctgccggat cttccggaat gtcgtgatca agaatgttga tcttcagtgt
    19201 ttcgcctgtc tgttttgcac cggaattttt gagttctgcc tcgagtttat cgatagcccc
    19261 acaaaaggtg tcatattcac gactaccaat accgattgcg ccaaagcgga ccgcagaaag
    19321 atcgggcttc tgttcctgca atgcttcata gaaaggagaa aggttgtccg gaatatctcc
    19381 ggcaccgtgg gtggagctga taatcagcca gatccccgag gcagataaat cttccaacag
    19441 cggaccgtgc agcgtttcgg tggtaaaacc cgcctcttcc agcttttcag ccaggtgttc
    19501 tgctacatat tcggcaccgc cgagggtgct gccgctgata agagtgatat ctgccataaa
    19561 ccgccacctt tattaagagt ggcgtattgt acgctgtgaa cgcgttggga tctacctgtg
    19621 gaaaagtatg ggattaaaaa agccgattat ggcttgatgg tacgcatgat cgggttttgc
    19681 aggacgatca atgtctcggt ggactgaatt tcatcaattg tttggatctt gttgataagt
    19741 acatgctgga gagcgtcgat cgaacggcac attactttta taaagatgct gtagtggcct
    19801 gttgtgtaat aggcttcagt gacttcagca aggctttcca gctttgccag cgcggaaggg
    19861 tagtctttgg cgctctttaa tataatgccg ataaagcagc ctacgtcata accgagctgc
    19921 ttcgggctga catcaatacg cgccccggta atgatccccg cctgcttcat tttctctact
    19981 cgaacgtgaa tcgtccccgg actgacgcca aattgtttcg ccagttcggc gtaagcggtg
    20041 cgcgcattgc ccattaatgc ttccaggatg ccacggtcca gattgtcgat cagataattt
    20101 tccataggat tttcttatgc agattgatga ttcattctat tttagccttc ttttttaatg
    20161 aatcaaaagt gtgttaggct ttttattgaa tgattattgc atgtttgtcg gtttttgttg
    20221 cttaatcata agcaacagga cgcaggagta taaaaaatga aaaccgctta cattgccaaa
    20281 caacgccaaa ttagcttcgt gaaatctcac ttttctcgcc aactggaaga acgtctgggg
    20341 ctgatcgaag tccaggcgcc gattcttagc cgtgtggggg atggcacgca ggataacttg
    20401 tcgggctgtg aaaaagcggt gcaggtaaaa gtgaaagctc tgcctgatgc ccagttcgaa
    20461 gtggttcatt cactggcgaa gtggaaacgt cagaccttag ggcaacacga cttcagcgcg
    20521 ggcgaagggc tgtacacgca catgaaagcc cttcgccccg atgaagaccg tctttctccg
    20581 ttgcactcgg tctatgttga ccagtgggac tgggaacgcg taatgggcga cggtgagcgt
    20641 caattctcga ctctgaaaag cacggtagag gcgatctggg cgggaattaa agcaaccgaa
    20701 gctgcggtta gcgaagagtt tggcctggca ccgttcctgc cggatcagat ccacttcgta
    20761 cacagccagg agttactgtc tcgttatccg gatctcgatg ccaaagggcg tgagcgggcg
    20821 atagcgaaag atcttggcgc ggtattcctt gtcgggattg gcggcaagct gagcgatggt
    20881 catcgccacg acgtgcgcgc accggattat gatgactgga gcaccccgtc agagctgggt
    20941 catgcgggtc tgaacggcga tattctggtg tggaaccctg tactggaaga tgcgtttgag
    21001 ctttcttcca tggggatccg cgtggatgcc gacacgttga agcatcagtt ggcgctgacc
    21061 ggtgatgaag atcgtctgca gctggaatgg catcaggcgc tgctgcgcgg tgaaatgccg
    21121 cagaccatcg gcggcggtat cggccagtct cgtttgacca tgctgctgct gcaactgccg
    21181 catatcggcc aggttcagtg tggtgtatgg ccagctgctg ttcgcgagaa cgtcccttct
    21241 ctgctgtaat aatttatcgc cgccagcgtc tgagcaggcg gcttcgcatc ccggtatcaa
    21301 agcgccagat atgatcgaaa atgcgcatga tgccgggttt gccgtgtgcc gacatcgcca
    21361 cggcatgaaa gcgatgctga tgtacccgct gcagctcttt cactttactc gtcacgtcgt
    21421 caggcaaccg ctgagcgata aaatcagaaa tcaccaccgc atcggcatca aaccattccc
    21481 tgctttgcaa gcgttccata atggcgcgaa aacaactggc aagatcggtg ccgccacgaa
    21541 actgctggct taaaaaacgg attgcttgtt cgatgccttg ggggcctgaa agctcataac
    21601 ggacgatctc ggtggaaaat agcataatat agcagcgccg gttttcagcg agagcaatgc
    21661 gcatcaaggc caggcagaac gctttcgcac actgttcatt aaagccgccc attgagccgg
    21721 aagtatccac acagacaata aacggcccgc gcggctgttc atcgtaatct ttatgtacca
    21781 ccggacgttc gatcactttt tcacgccacg actcaccgtg caggcgatag gtgagcaact
    21841 gtttttccac cagccgacgg taaaactcat actccagttc cgttatccct agtgtcgcca
    21901 gttctggcgg cagaagacgt aaaatatcat cgctttgttg cagaccatca acctgctcag
    21961 gaaccgtcgc cggttcgcgc accatggtgc ggaaggtttc catctgcgca tcgttgcgcg
    22021 gtattgattt ggcttcccga gaacgcccca gctgctctgc caggcgtttc agttccggct
    22081 gttcgttaag aaattcaccg tatttcacaa tcaactgata gtcgccacgt ttaagctgac
    22141 cggcgctcat atcccacaga cgaccagctg cagtattgtt atctgcgaga atcggttcaa
    22201 gttgtccgct cagcgtcatg cgttcttgaa cttcactcaa cagttgttcg cgttcttctt
    22261 ctaatagctg ttgatttaac gtcgttgctt gcacgatcag acttaaacgc caacgctgga
    22321 gaaaaagcgt gtgtaacgct gaagttattg tgctgttagc atcaaccaac tgccgggctt
    22381 gttctgccca tggggaattc agacgatgca gtaagtccag gatctgtggt agctgcacaa
    22441 taaactgcgg cgtggagagg agctggcttt gctgatagca catcacctct tcggtgagtt
    22501 ccggcgggac tcgggcatct ttcagccgac tgcgcagcgc ctcacgccag cggggaacat
    22561 catcagtgat tgccgccttc aggcgtggga atttttcaaa gaagaccgcc agctgcggtg
    22621 aggccagcag tgcgatgatc atctcttcga tcaatccctc ttcgctgacg gccagcatca
    22681 cattaagcgt atccagcgtt agcattgttg tgcctggtga atctgtgcac cgacatcctg
    22741 taggctggct tcaatgcgac ctaaccagtc gccagggata aacagacatt tttgctgttc
    22801 gctgaataaa gcgtgttgct tgcgccagtc gctttcaagc tcttccagtt gttgttttat
    22861 ttcaccaggc agaccttcag ccgatgaacc gggaagtgcc agcgtactgc cttgtaaact
    22921 tacatcgcga acgacaagat gttgggcgct atcaacttcc agattcagtt tctgggcaaa
    22981 gccgataccg ttcagtttac cgcgaatttc accacctttg ctcagccact gttccagcgc
    23041 gctacgctca aaggagatat gaaccacttc catatcatgc agttttaacg gtttttgcag
    23101 cagtagagtc agagtggaag cagtaacgtt aacaggaagt tgatactgct gacgacggct
    23161 gaaaatgccg cccagacgaa ttaccgttaa ggctgtttta tcgctttgtt gctgctggag
    23221 ttgcaggtga cgttgcacaa tcgcgccaag gcgagtcaac atcccttgct gttgccaggc
    23281 atgaccggtc atcaatacat caatttgttg ttgtatcaaa ttcaggcttt gcgcgtcata
    23341 ccacaggcaa tctttcagca aaatgagatc taccggggca acagcactgc gaccgctaaa
    23401 aaaggcgctg gcctgcaata atcggatcgc ttttttccag cgacgatccg agacataagg
    23461 cgcatccggt aatttatcca gttgcaggcg cagcataaaa atcagctcaa atacatgatc
    23521 gggcagcgta atttcaccaa tctctttctg ccagcgttca tattcttcat ctgtgacctg
    23581 caaggcatca ggaaccggat tgtcgttttc atcctgttga ctggtcagca tggagcggaa
    23641 attcgcttta tcctgcactt tatctaacca cagacgaatc agcatgcggt catataacgc
    23701 ttccagactg ctgtctgctt ccggcagctc gttggaggcc gccaccagca gacgcatcgg
    23761 gattttttct acgtgtgcac cgttgcggaa ctggcgctcg ttaatggcgg tgagcaaggt
    23821 attaagaatt gccgggcccg ctttccagat ctcatccaga aagacaattt ctgcttccgg
    23881 caggtaaccg ctggttaaac gttcatagcg cccttcatct tttagcgcct gaatagaaag
    23941 gggaccaaaa acttcttccg gcgtggagaa gcgggtcatc agatattcaa acgcgcgggc
    24001 attctgaaag gcgaatttta agcgccgggc gatcaaactt ttggcaatac ctggcgggcc
    24061 aaggaggaac acactttcac cacttaatgc cgctaataaa cacaagcgga tggcgtggct
    24121 acgttcataa agcccctttt ccaacgaact gctcaggcgg gaaattcttt ccgctaataa
    24181 atgagggtga gccataatga agtggcgtcc tttcgtcaaa agttctgcgt aaattgcgag
    24241 tatagacgtt tcttgctggt ggctaaaata gtctcaaag
//
LOCUS       DAJTYZ010000059        23951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000059 DAJTYZ010000000
VERSION     DAJTYZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.059"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(38..400)
                     /locus_tag="OO596_002217"
     CDS             complement(38..400)
                     /locus_tag="OO596_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6718065.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="OO596_002218"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="OO596_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6718066.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="OO596_002219"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="OO596_002219"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6718067.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="OO596_002220"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="OO596_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6718068.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /locus_tag="OO596_002221"
     CDS             complement(4827..5036)
                     /locus_tag="OO596_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718069.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="OO596_002222"
     CDS             5321..5683
                     /locus_tag="OO596_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6718070.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6226..6904
                     /locus_tag="OO596_002223"
                     /pseudo
     CDS             6226..6904
                     /locus_tag="OO596_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6948..11798
                     /locus_tag="OO596_002224"
     CDS             6948..11798
                     /locus_tag="OO596_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR6718071.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12166..13821
                     /gene="lldP"
                     /locus_tag="OO596_002225"
     CDS             12166..13821
                     /gene="lldP"
                     /locus_tag="OO596_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6718072.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13821..14597
                     /gene="lldR"
                     /locus_tag="OO596_002226"
     CDS             13821..14597
                     /gene="lldR"
                     /locus_tag="OO596_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6718073.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            14594..15784
                     /gene="lldD"
                     /locus_tag="OO596_002227"
     CDS             14594..15784
                     /gene="lldD"
                     /locus_tag="OO596_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6718074.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            15980..16453
                     /gene="trmL"
                     /locus_tag="OO596_002228"
     CDS             15980..16453
                     /gene="trmL"
                     /locus_tag="OO596_002228"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6718075.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16506..17327)
                     /gene="cysE"
                     /locus_tag="OO596_002229"
     CDS             complement(16506..17327)
                     /gene="cysE"
                     /locus_tag="OO596_002229"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6718076.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17407..18426)
                     /gene="gpsA"
                     /locus_tag="OO596_002230"
     CDS             complement(17407..18426)
                     /gene="gpsA"
                     /locus_tag="OO596_002230"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6718077.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18426..18893)
                     /gene="secB"
                     /locus_tag="OO596_002231"
     CDS             complement(18426..18893)
                     /gene="secB"
                     /locus_tag="OO596_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6718078.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18956..19207)
                     /gene="grxC"
                     /locus_tag="OO596_002232"
     CDS             complement(18956..19207)
                     /gene="grxC"
                     /locus_tag="OO596_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6718079.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19349..19780)
                     /locus_tag="OO596_002233"
     CDS             complement(19349..19780)
                     /locus_tag="OO596_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6718080.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20025..21569
                     /gene="gpmM"
                     /locus_tag="OO596_002234"
                     /gene_synonym="pgmI"
     CDS             20025..21569
                     /gene="gpmM"
                     /locus_tag="OO596_002234"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6718081.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21579..22862
                     /gene="envC"
                     /locus_tag="OO596_002235"
     CDS             21579..22862
                     /gene="envC"
                     /locus_tag="OO596_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6718082.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22866..23825
                     /locus_tag="OO596_002236"
     CDS             22866..23825
                     /locus_tag="OO596_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6718083.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         6044 a         6108 c         6212 g         5587 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgatat
     5821 tctgaaaatg catttttacc gaaatagcca cctataaaca ataaaaatat atagaagata
     5881 tcatttatta attaacaagc agttacgtct ctttttttcg gatttaattg tgaaatttta
     5941 atcatcatat tcatacaata aattatctgt aaattttttc ccagacatgg tttctttcat
     6001 atttgacatt tattaataat catcattaga atccacgaaa ataatatatt catacaaaca
     6061 cagacatctt ttgaaacgaa tgatacattt ttatgatgaa tgcttaaaac aatatttaat
     6121 cgacacacat cactgtcatt aatgtaaagc aacatagatc accaataata caatggacaa
     6181 ctctaccggc attgatcgct ggtcggagga aataaggatt tttacatgaa cttgaagaag
     6241 atttttttca gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg tgactacatt
     6301 gaagaaggca agccagaaag tagtttattg aaacaacaag aagaacatta aaattgatct
     6361 acttgaaaaa caacaagcac agctaaagag tcaactggaa actattcaaa aacaacaaac
     6421 tggcataata agcagtacaa agactttaac tcacgtaatt aaatccgtta aagatcaaca
     6481 aaacactttt atttttaccg agtttaaccc agcaaaaacg aagtatttca tcctgaacaa
     6541 tggttcggtg gctttagcgg gtcgagtgtt atccattgac gccacagaga atggcagtgt
     6601 tattcatatt tcactggtca acttattaag tacacctatc tcaaatattg gttttaatgc
     6661 gacatggggt ggtgaaaaac ctgtcgatgc caaagagttt gcccggtggc aacaattact
     6721 tttcaacaca tcaatgaaat ccacattgaa attattacca ggtcaatggc aagacattaa
     6781 tttgaccctg aagggtgtat cgcccaataa cctgggatat ctgaagttag ccatcaacat
     6841 ggaaaatatt cagttcgaca atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa
     6901 ataaatgcag ttagtacgct tataacaagg aatagcgagc atcattaatg aacaaaatat
     6961 ttaaagttat ctggaaccct gcgacaggga attatactgt taccagcgaa acggcaaaaa
     7021 gccgtggcaa gaaatctggg cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa
     7081 tgttgtcgtc gtttggggca ttggcgaatg ccgggaatga caacggccag ggtgttgatt
     7141 acggtagtgg atcagctggc gacagctggg ttgctatagg caaaggggcg aaagcaaata
     7201 cttttatgaa caccagtggt tccagtactg ctgtgggtta tgacgctata gctgaaggcc
     7261 aatatagctc tgccatcggg tcaaaaaccc atgcgattgg tggtgcatca atggcctttg
     7321 gggttagtgc aatatcagaa ggcgatagaa gtatagcact gggtgcctct tcgtattcat
     7381 tgggccaata ctcaatggcc ctcggccgtt attcaaaagc attgggtaaa ttgtctattg
     7441 ctatggggga ctcttccaaa gcggaaggag caaacgccat tgccctggga aatgccacta
     7501 aagctactga gattatgagt attgctcttg gcgacaccgc caatgcgtca aaagcgtatt
     7561 caatggcgct gggagcaagt agcgtcgcat ctgaagaaaa cgcaattgcc ctggggcgta
     7621 gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt gccagcgcag
     7681 cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact gctattggca
     7741 ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc cttgccgata
     7801 aagtcaatac tatcgcatta ggaaatggca gccaggctct ggcagataat gcaatcgcca
     7861 taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat ggtagccagt
     7921 cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt gataagagtc
     7981 ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt
     8041 ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa cgcaagatcg
     8101 ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat ggttcacagc
     8161 tttatgccat tagcgactcg gtagcaaaaa ggcttggagg aggggctgca gtagatgttg
     8221 atgacggtac tgttacagca ccaacctaca atttaaaaaa tggtagcaaa aataacgtag
     8281 gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat
     8341 acagcgctgc tcatggtact agtagcccaa ctgccagcgt aatcaccgat gttgcggatg
     8401 gcacgatttc agcctccagt aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg
     8461 acgatgtcga agccaacacc gccaatatcg ctactaatac cagcaacatt gccacgaata
     8521 cagcaagtat tgccaccaat accaccaata tcaccaacct gacggattcc gttggtgacc
     8581 ttcaggctga tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc
     8641 aggataccac cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg
     8701 atgctgttaa cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca
     8761 atatcgccaa taacacttcc aatattgcca ctaacaccac caacatctct aatctgactg
     8821 agacggtgac taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca
     8881 cggcagctca tggcaccgag accaccagca aaatcaccaa cgttaaagat ggcgacctga
     8941 cgactggcag caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg
     9001 cgacgaatac caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg
     9061 tgactaatct tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag
     9121 ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa
     9181 ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct
     9241 acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa
     9301 tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta
     9361 gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac
     9421 acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact
     9481 ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg
     9541 cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg acgtacacca
     9601 ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat accactcttg
     9661 acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc gccgctcacg
     9721 gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc tctgctgcca
     9781 gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc gctgatgcgc
     9841 tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact tacaccattg
     9901 cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat aacgccctgc
     9961 tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat gacggtaaag
    10021 ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc gatgcagtga
    10081 acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa attatcaatc
    10141 agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt atcaactacg
    10201 ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt gtcggcgcta
    10261 cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt ggtcagaaca
    10321 gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc agccgtgtga
    10381 ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cgtggtgatt ggttatgaca
    10441 ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa taccgccaaa
    10501 tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc cagttgcaga
    10561 acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac tcaacggcag
    10621 aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact gtcgttaatg
    10681 gtaatgcagg tattggtatc ggtttgaaca ctctggttct agctgatgcg atcaacggca
    10741 ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg ggtaacggtt
    10801 ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac gcaccgcaga
    10861 actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc acaaacgtcg
    10921 cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc actgatgagc
    10981 gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact aatctggata
    11041 ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt agcaccaagt
    11101 acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat agcgttgcta
    11161 ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc ggttccgttg
    11221 cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt atcaccaacg
    11281 tagctgccgg tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa tcttctgaag
    11341 caggcggcgt gcgctacgac accaaagctg atggttctat cgactatagc aatatcaccc
    11401 tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc gtcaacaaca
    11461 acgacgcggt gaactacgcg cagttgaagc aaagcgtgca ggaaacgaag caatacaccg
    11521 atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag ttgagcggtg
    11581 gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg ggtgccagca
    11641 tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta ggtgtatcga
    11701 tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat agccagggtg
    11761 aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag ttagtgtgac
    11821 taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata aggcgccccg
    11881 ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac cagacatctc
    11941 cccccacaag aattggccct accaattctt cgcttatctg acctctggtt cacaattccc
    12001 caattaaaac tcacatcaat attgccaata cataacattt agttaaccat tcattgtcat
    12061 tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag
    12121 caacagactc attacacgat gtgcgtggac tccaggagac ctgcaatgaa tctctggcaa
    12181 caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc gcttcccatc
    12241 ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg
    12301 acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg
    12361 ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg gatcattatt
    12421 gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat cattcgctcg
    12481 tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg tttctgtttc
    12541 ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg
    12601 ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat tgttaacacc
    12661 gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca agtaacaggc
    12721 atcgacagct ttgagattgg ccagatggtg gggcggcagc taccgtttat gaccattatc
    12781 gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaagga gacgtggcct
    12841 gcggtcgtgg ttgcgggcgg ctcgtttgcc atcgctcagt accttagctc taacttcatt
    12901 gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc
    12961 ctcaaacgct ggcagccagt gcgcgtattc cgttttggtg atttgggggc gtcaaagatt
    13021 gatatgacgc tggcccacac cggttacact gcgggtcagg tgttacgtgc ctggacaccg
    13081 ttcctgttcc tgacagctac cgtaacactg tggagcatcc cgccgtttaa agccctgttc
    13141 gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa
    13201 ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc cgtgtttaag
    13261 tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct ctcgattgtc
    13321 tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct gaaagaactg
    13381 gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc gaactattcc
    13441 ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt caccttcttc
    13501 tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc atctaacgcc
    13561 ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga tctgttgctg
    13621 gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca atctatcgct
    13681 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
    13741 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc
    13801 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
    13861 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
    13921 agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actgcgcgag gcgctggcaa
    13981 aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt attcgctggc
    14041 gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg atggccgatg
    14101 atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcat
    14161 ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt tgctttgaag
    14221 caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt catctggcga
    14281 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
    14341 tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg gttttttcac
    14401 aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat gccgacgggg
    14461 cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa cgattcgatg
    14521 aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat gagcattcga
    14581 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat
    14641 tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat acacgctgcg
    14701 ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga aaaacatgtc
    14761 cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tagcactggc
    14821 accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag ccaaagcggc
    14881 ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
    14941 cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg atcgcggctt
    15001 tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt
    15061 ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga gcggcccaaa
    15121 tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
    15181 cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca aaccgaccgg
    15241 actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga
    15301 ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga tcctcgatcc
    15361 ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt ctaaccacgg
    15421 tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc
    15481 ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt
    15541 gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct
    15601 ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa aagagatgaa
    15661 agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag attcgctggt
    15721 gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag ggaatgcggc
    15781 atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga ccgttcggcg
    15841 ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc tcgcccctcc
    15901 ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg ctatcctgcc
    15961 cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa ttccgccaaa
    16021 tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctgcata tcatcgaacc
    16081 gatgggattt gcctgggacg ataagcgcct gcgccgcgcg gggctggact atcacgagtt
    16141 taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa atccccagcg
    16201 cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct atcaggatgg
    16261 cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc ttgatgccct
    16321 gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca tgaatctgtc
    16381 caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc cgggagcgtt
    16441 gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg cacgatcaca
    16501 ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa atgctggtcc
    16561 atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg aacgccagcg
    16621 gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat cttcgcgccg
    16681 cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac accttcacga
    16741 attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac agattgcaga
    16801 atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt ggcgtggtcg
    16861 agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa cgtcacagaa
    16921 acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa ccagtgaccg
    16981 atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt tgagtattta
    17041 tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga ggcgatcatt
    17101 tccgggtcag cggcgtaggc ttcttccacc acttcacgga tagcaatagc aggcataatt
    17161 ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac taccaaggtt ttcgtgcttg
    17221 agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag cgttctggct
    17281 tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc ttactccaca
    17341 cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa caaaggttcc
    17401 ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataacg tcaatgctgc
    17461 ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta ttggcatttc
    17521 tacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc cttccaccac
    17581 ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca tcatgccaaa
    17641 acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca gccccgccat
    17701 gcccataaag gtggcaggat cggcacccag cgccgcgcca agacgcgaca tttcagccag
    17761 cccacgggtg atcagtgccg tacgcgcatt cgcaccaaaa ccgataccgt cggacatccc
    17821 cgcgccaatg gcaatgacgt ttttcaccgc accgccaagc tgcacaccga tgaaatccgg
    17881 gttgctgtaa acgcggaaac ttttgccaca gtgcagcaat tgttggagat catcggcaaa
    17941 ggtctggtcg gtagaggcca acgaaatagc cgtcggtaaa cctgccgcca gttctttcgc
    18001 aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct cacgcgccac
    18061 gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca ccagacgcgc
    18121 atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat ggctgggtac
    18181 gacgacgaga atattacggc tggctgccag cgcagtggcg agatcgcttt caagatggag
    18241 cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc gttcaagcgt
    18301 tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc ttgccagggt
    18361 aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag cattacgttg
    18421 gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg caaatagttc
    18481 atgaacagcg catcgaagtt aatcggcgca aggttcagtt gcgggaatgt accgcgggat
    18541 accacgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca gtatgcgccc
    18601 aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc gccctgctga
    18661 acttcacaca ggaacgcggt ttcttcgccc agggaggccg ttacggtaac acgcagtacc
    18721 acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag tttaacttct
    18781 ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga gatatccttg
    18841 gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga catgtgtaga
    18901 aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc acacgttatt
    18961 tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca cagccgccaa
    19021 tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt ttgatcatct
    19081 cttcacgctt ggcggcattg ccatcgatcg gcaactcctg aaaactcacg cccttgctgc
    19141 tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag atttcaacat
    19201 tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg gcggctgcaa
    19261 aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt gcagctaacg
    19321 gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc aagttttcgc
    19381 cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca gctttcgtca
    19441 gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata accggtttgt
    19501 ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc aacagattga
    19561 tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc acaaccacag
    19621 cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc actttcgagg
    19681 tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag gcgatactca
    19741 gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac aactcccgac
    19801 gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa tacagccagc
    19861 gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa tctggcatcg
    19921 gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga ttcagctgta
    19981 gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg gtttctaaaa
    20041 aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag caggataacg
    20101 ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc
    20161 tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt aactccgaag
    20221 taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact cgtctggacg
    20281 ttgaaatcaa agatcgcgct ttctttgcta atccagtgct gactggcgcg gtagataaag
    20341 cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc ggcgtacaca
    20401 gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa
    20461 tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct gaatcctcgc
    20521 tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg tccatcattg
    20581 gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa gcctatgacc
    20641 tgctgactct ggcgcagggt gagtttcagg ccgatactgc cgttgctggt ttgcaggcag
    20701 cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc
    20761 agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc cgtgctgacc
    20821 gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc gcgcgtaaga
    20881 aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc aaaactgcgg
    20941 ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg aaaaacgaca
    21001 aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc ttcttcaacg
    21061 gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca ccgaaagtgg
    21121 ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg
    21181 ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc gacatggtag
    21241 gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat cactgcgtgg
    21301 aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc gctgaccacg
    21361 gtaacgctga acagatgcgc gatccggcaa cgggtcaggc acacacggca cacaccaacc
    21421 tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca ggcggcaaac
    21481 tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg caagagatga
    21541 ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag gcgattaata
    21601 ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg
    21661 ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag cgtgaccaac
    21721 tcaaatctat tcaggccgat atcgccacaa aagagcgcgc ggtacgccaa aagcaacaac
    21781 aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct gaagccaccc
    21841 gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt gatgagatga
    21901 acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc ctcgccgcac
    21961 aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt ctcagcggtg
    22021 aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac caggcgcgac
    22081 aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt cgccatgcag cgtgctgaac
    22141 tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc caacaggcga
    22201 aactgactca ggctctgaac gagcgtaaaa agacgctggc ggggctggaa tcttccatcc
    22261 agcaaggtca gcagcagcta agtgaactgc gtgccaacga atcccgtctg cgtaacagta
    22321 ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt gaggctcagg
    22381 cagttcgcga caggcagaaa gaagcgacgc gcaaaggcac cacctacaaa ccgaccgaaa
    22441 gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt caggcattct
    22501 ggccagttcg tggccctacg ctgcatcgct atggcgagca gctacagggt gaattacgct
    22561 ggaaaggaat ggttatcggt gcctctgaag gtactgaagt taaagcaatt gccgacggtc
    22621 gggtgattct ggctgactgg ctgcaaggct acggtctggt ggtggtggtt gagcacggta
    22681 aaggcgatat gagtctttac ggctataatc aaagcgcgct ggtgagcgtt ggttcgcagg
    22741 ttcgtgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggc agaccttcac
    22801 tctatttcga aattcgccgc cagggtcagg ccgtcaatcc acagccgtgg ttgggaagat
    22861 aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct
    22921 cccctgtaat tgctggcaaa cttgccatcg tcattgatga ttttgggtat cgcccgaaca
    22981 acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta cccgattcac
    23041 cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg ttgattcatc
    23101 tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctgcgc ccggagatga
    23161 gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc tatgccgtgg
    23221 ggatcaataa ccacatgggc agcaagatga cctctaacct gtttggtatg cagaaagtga
    23281 tgcaggcgct ggagcgttat aatctttact tcctcgacag cgtaaccatt ggtaataccc
    23341 aggcgatgcg cgccgcgcaa ggcactggcg tgaaggtgat caaacggaag gtgttcctcg
    23401 acgattcaca gaacgaagcg gacatccgtg tgcaattcaa tcgcgcaatt gacctagcgc
    23461 gtcgcaacgg atcaaccatt gccattgggc atcctcaccc ttcaacggta cgcgtgttgc
    23521 aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc agcttgctga
    23581 atgaaccgca ggttgatacg tctacaccgc cgaaaaacac tgtgcccgac acaccgcgta
    23641 atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct gtttatgcta
    23701 atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc gtttacttcc
    23761 agcatcagtg gcaaggctgg ggcaaacagc ctgaagcggc gaagcttaac gctagcgtaa
    23821 attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa
    23881 gcggaaagac tacagtaacg cctgataacc cacctttacg ctgcgcacat tggtaatcag
    23941 gcgcttatac a
//
LOCUS       DAJTYZ010000060        23870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000060 DAJTYZ010000000
VERSION     DAJTYZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.060"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(96..1349)
                     /gene="amiC"
                     /locus_tag="OO596_002237"
     CDS             complement(96..1349)
                     /gene="amiC"
                     /locus_tag="OO596_002237"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6718084.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1581..2912
                     /gene="argA"
                     /locus_tag="OO596_002238"
     CDS             1581..2912
                     /gene="argA"
                     /locus_tag="OO596_002238"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6718085.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2974..4800)
                     /gene="recD"
                     /locus_tag="OO596_002239"
     CDS             complement(2974..4800)
                     /gene="recD"
                     /locus_tag="OO596_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6718086.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(4800..8342)
                     /gene="recB"
                     /locus_tag="OO596_002240"
     CDS             complement(4800..8342)
                     /gene="recB"
                     /locus_tag="OO596_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6718087.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8335..11223)
                     /gene="ptrA"
                     /locus_tag="OO596_002241"
     CDS             complement(8335..11223)
                     /gene="ptrA"
                     /locus_tag="OO596_002241"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6718088.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALLGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11399..14767)
                     /gene="recC"
                     /locus_tag="OO596_002242"
     CDS             complement(11399..14767)
                     /gene="recC"
                     /locus_tag="OO596_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6718089.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14780..15103)
                     /locus_tag="OO596_002243"
     CDS             complement(14780..15103)
                     /locus_tag="OO596_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6718090.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15088..15495)
                     /locus_tag="OO596_002244"
     CDS             complement(15088..15495)
                     /locus_tag="OO596_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6718091.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15492..16055)
                     /locus_tag="OO596_002245"
     CDS             complement(15492..16055)
                     /locus_tag="OO596_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6718092.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16046..16516)
                     /locus_tag="OO596_002246"
     CDS             complement(16046..16516)
                     /locus_tag="OO596_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6718093.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16701..17495)
                     /gene="thyA"
                     /locus_tag="OO596_002247"
     CDS             complement(16701..17495)
                     /gene="thyA"
                     /locus_tag="OO596_002247"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6718094.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17502..18377)
                     /gene="lgt"
                     /locus_tag="OO596_002248"
     CDS             complement(17502..18377)
                     /gene="lgt"
                     /locus_tag="OO596_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6718095.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18528..20774)
                     /gene="ptsP"
                     /locus_tag="OO596_002249"
     CDS             complement(18528..20774)
                     /gene="ptsP"
                     /locus_tag="OO596_002249"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6718096.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20787..21317)
                     /gene="rppH"
                     /locus_tag="OO596_002250"
     CDS             complement(20787..21317)
                     /gene="rppH"
                     /locus_tag="OO596_002250"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6718097.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            22002..22691
                     /gene="mutH"
                     /locus_tag="OO596_002251"
     CDS             22002..22691
                     /gene="mutH"
                     /locus_tag="OO596_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6718098.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22760..23473
                     /locus_tag="OO596_002252"
     CDS             22760..23473
                     /locus_tag="OO596_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6718099.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23611..23829
                     /gene="ygdR"
                     /locus_tag="OO596_002253"
     CDS             23611..23829
                     /gene="ygdR"
                     /locus_tag="OO596_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6718100.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         5707 a         6537 c         5962 g         5664 t
ORIGIN      
        1 caaatccggc ccccgcaacc aattattgaa caccctaacg ggtgtttttt tgtttctact
       61 ctcccataaa aaaagcgcca ttcagcgcct ttttatcatc cccttctcgc cagcgttgcc
      121 ccatcggcaa aatacgcttt aatccccgca agaatagact ccgcaacttc ctgctggaaa
      181 gtcgccgttt tcagtttacg ctcttcctca acgttactga taaacgccgt ttcgaccaga
      241 atggagggaa tatctggtgc ctttagtacg gcaaacccgg cctgttcaac ttgattttta
      301 tgcagcttgt tgattttacc gagcttattc agcactgctt taccaaactt aaggctgtca
      361 gcaatggtca gcgactgcac catatcgaac atggtgtggt cgacatagcg gtcaccgctt
      421 ttgctcacgc caccaatcaa gtccgaggcg ttctgggttt gtgccagata ttttgccgca
      481 gtactggttg cacctttggt tgagagcgca aacaccgagg aaccgctcgg ctggcgactg
      541 gtaaaggcgt cggcatggat agagacaaac aggtcagcac gctgtttctg ggcttttgct
      601 acgcgcactt gcaacggaat gaagatgtct tcattgcgcg tcatgtacac cttcatattg
      661 ccctctttct cgatcagaga gcgcaaacgg cgagctattt gcaataccac atctttttcg
      721 cgcgttttgt atttccccac cgcaccggag tcttcgccac cgtggccagg gtcaagcata
      781 atgacaatcg gacgatcgcg ccctgcttta cctggttgtg gaccactttg cgctggcggc
      841 acctgctttt cgaggtcgcc tttgttgtaa tcctccagca gcgccagcag cgggtcctgc
      901 atatcctgtg cattggccgg atagaggtcc atcaccagac gctctttaaa cccggcgacc
      961 ggcgcaaggg caaacagctg cggttttacg ttttgcttta attcaaaaac catccgtacg
     1021 gtttgcgggt caaattgccc gacgcgcgcc gacttgatga acgggtcgtc tgcacgaatt
     1081 tgtgccgcca tccccttgag taccgagttc aggtttacat cttcgatatc caccaccacg
     1141 cgttcagggt tactcaacgc gaactgctta tatttcagct gacgatttga ttctaccgtc
     1201 acgcgggtgt agctggacgc aggccagacg cgcaccgcca cgacctggct aaccgcagcc
     1261 aggctgacct gacttacgct caatagccac atagcgcccg cgccttgcag taaacgacga
     1321 cggctgattg cagtgttgga tcctgacatg cctctcccga gcaaaagaaa tctaagctgt
     1381 gtaacaagta aacgactaat ttgaccggaa actttagcga aagacgcata atctgtcatc
     1441 taataaacgg taaacattct ttttatattc acggcattac tgataaaaaa gtcgctctcg
     1501 cataaaattt acacttgcac cctgcgaaaa aacagaataa aaatacaata atttcgaata
     1561 atcatgcaaa ggggtgtgcc gtggtaaagg aacgtaaaac cgagctggtc gagggattcc
     1621 gccattcggt tccctatatc aatacccacc ggggaaaaac gtttgtcatc atgctcggcg
     1681 gtgaagccat tgagcatgag aatttctcca gtatcgttaa tgatatcggg ttgttgcaca
     1741 gcctcggcat ccgtctggtg gtggtctatg gcgcacgtcc gcagatcgac gcaaatctgg
     1801 ctgcgcatca ccacgaaccg ctgtatcaca agaatatacg tgtgaccgac gccaaaacac
     1861 tggaactggt gaagcaggct gcgggaacat tgcaactgga tattactgct cgcctgtcga
     1921 tgagtctcaa taacacgccg ctgcagggcg cgcatatcaa cgtcgtcagt ggcaatttta
     1981 ttattgccca gccgctgggc gtcgatgacg gcgtggatta ctgccatagc gggcgtatcc
     2041 ggcggattga tgaagacgcg atccgtcgtc aactggacag cggtgcaata gtgctaatgg
     2101 ggccggtcgc tgtttcagtc actggcgaga gctttaatct gacctcggaa gagattgcca
     2161 ctcaactggc catcaaactg aaagctgaga agatgattgg tttttgctct tcacagggcg
     2221 tcactaatga cgacggtgat attgtctcag aacttttccc taacgaagcg caagcgcggg
     2281 tagaagccca ggaagagaaa ggcgattaca actccggtac ggtgcgcttt ttgcgtggcg
     2341 cagtgaaagc ctgccgcagc ggcgtgcgtc gctgtcattt aatcagttat caggaagatg
     2401 gcgcgctgtt gcaagagttg ttctcacgcg acggtatcgg tacgcagatt gtgatggaaa
     2461 gcgccgagca gattcgtcgc gcaacaatca acgatattgg cggtattctg gagttgattc
     2521 gcccgctgga gcagcaaggt attctggtgc gccgttctcg cgagcagctg gagatggaaa
     2581 tcgacaaatt caccattatt cagctcgata acacgactat tgcctgcgcc gcgctctatc
     2641 cgttcccgga agagaagatt ggggaaatgg cctgtgtggc agttcacccg gattaccgca
     2701 gttcatcacg gggcgaggtt ctgctggaac gcattgccgc tcaggcgaag cagagcggct
     2761 taagcaaatt gtttgtgctg accacgcgca gtattcactg gttccaggaa cgtggattta
     2821 ccccggtgga tattgattta ctgcccgaga gcaaaaagca gttgtacaac taccagcgta
     2881 aatccaaagt gttgatggcg gatttagggt aagcgatgaa aatcgccgga tgcggcaggc
     2941 gtaacactcg tgcgtcgcat ccggcaatta cgtttatccc cgcgaactaa acaacgccgc
     3001 cagaccactg cgccgctcag tacgagtggc gattgccgca cttaatatac gctcatcggc
     3061 atacagcgac agacggcgac gcgcgcgggt caccgcggtg tataccagct ctcgcgttac
     3121 tatcggcgtg cgttggctcg gtaaaatcaa cgccgcatgg tcgaactccg atccctgcga
     3181 tttatgtacc gtcatcgccc acgtagtttc gtgctctggc aagcgactcg gttgcacaga
     3241 cttaatattg ccgtccggca tcgcaaacca gacgcgcgtc ccctgcccgc gatccagcgc
     3301 aataccgata tcgccattaa acaacccaag cgcgctgtca ttacgggcaa tcatcaccgg
     3361 tcgaccttcg taccaacgag agtgcgaatg acgatgaatt ttgcgcttct gttgcataaa
     3421 ctgctcaatt cgctcattca gtccagccac gccaaatggc ccttcccgca gggcgcacaa
     3481 aagttggtac tcattgaacg cctgaatgat taaatccggc tcggcacgcg cttgcagcag
     3541 atccagataa cgtccataac ccgcaagagc ttcctcaagc atcgcaatat aatcttcgcc
     3601 gctctgtaaa agccgttttt caatatcagt aaaatcctgc tgaaaaacgg ttttcactgc
     3661 cgttttatca ccacggttga tcgccgcagc taactgacca atgccagaat cgctgccgaa
     3721 acgatagctt ttttgcagca ggcagagact gtcgcgcaaa gatgccgctt ctgtgccggt
     3781 tcctgccgga acgtgagttc ccgtcaggcg gcttagctgc ccggcacgct cggcggtaaa
     3841 gccggcattg gcataagcgc agatatcgcc cagcacagcc ccagcctcaa ccgaggccag
     3901 ttgatcacga tcgccgagaa agatcactcg cgcatgatcg ggcaaggcgt cgatcagtct
     3961 cgacatcata ggcagatcga tcattgacgc ttcatctacc accagcacat caagatgcag
     4021 cgggttaccg gcatgatgac gtaaacgctg gctacccggc tgcgcgccca gcaatcggtg
     4081 caaagtgctg gcatcttccg gaatgcgttt cttttgttca tcggtcagcg gtaactgtcg
     4141 caaagccttg ccgagagatt cggttaagcg cgcggcagct ttacccgttg gtgcagccag
     4201 acggatacgg cagcgttcgc cgtcggccat ttgaattaac gctgccagca acttcgccac
     4261 ggtggtcgtt ttaccggtgc ctgggccgcc ggaaatcacc gagatccgcc gcgtcagcgc
     4321 cactgctgcc gccacttttt gccagttaat ttcatcgctt actggaaaaa gtttgtccag
     4381 ggtttgcgcc agtagagctt catcaacctc aatggcatga ttcacctcat tgaagaagcg
     4441 cgctaccgtc cgctcgttac accacatgcg attcaaataa agacgatcgc cacagaggat
     4501 catcggcgtg ggttcatctc ccctgctgac cgcttgagaa gccagcaagg attcttccca
     4561 attttgtagc tcaccgattt cactgacaca ggtcgccaac agcggatgcg acgcctcgtt
     4621 attttccagt cgtgaaagcg gcaaacagac gtgtccctct ccggcatcat gacttaacag
     4681 tgccgccgcg agggtgacgg caggatgttc atctcccgcc acggtcaggg caaattgcac
     4741 atccagcggg cgtagctgtt tgtgctccac agcttccagt aattgctttt gcaatttcat
     4801 tacgcctcct ccagggtcat accggcaaac atctcatcca tcagggcaat caacccggcg
     4861 ttgggtcggg ttgtgtaaat cccttgttgc ggatgttctt tatcaacgcc acgcaggaac
     4921 agatagataa cgccgccaaa gtgacgatca tagtcgtaat cagcaatgcg atggcgcaga
     4981 tagcgatgca gcgccagggt ataaagctga tattgcagat catagcggtg tgcctgcatc
     5041 gctgctgcca tagcctgttg ggtgtaagcc gaactgtctt cacccagcca gttggattta
     5101 tagtcgagca ggtaataacg cccttcgtgg cggaatacca ggtcgataaa gccttttaac
     5161 atgccgcgta cctgcatgaa ctccagcggc gggcagccag cggatagcgg gtcaaactgg
     5221 cgaattagtg catcaagctg gctggcgatg agcggttcgc taatcggcag gtaaaactcc
     5281 atctccacct gtttatcgcg gtcggaaagc tgactcaggc taacacccgt ttcattgaga
     5341 ggtgcctgga ggacagccgt gatccactcg gtcaacaccg gttcccactg cgattcaaag
     5401 ccgccgagtt ccagtttttc ctgtacccag ttcgggtcaa ccggctgggt aaaatcgagg
     5461 tcttcaaaca aactgtgcaa gaatgtcccc ggtgacgcac cgcgcgggaa ctgatgcggt
     5521 gttaacgtcg gttcttcaac gacgctgacc acgcctgcgg catcaacatc cagccgtggc
     5581 atcagatcct gagcgatacc gtgaccacgc tgctgcaagc cggagtagct ggtgacacgc
     5641 cagttatcgc cgggcaatcg ttgtagtgtc ctcgcattca gttctgcagt taaagcatca
     5701 ttaacctgcc agggctggtt atcaccagtt tgtgccgttt gccaggcaat atcatcatcg
     5761 cataacgctt caatacaggt gcgaagccct gccgcatctt gcggttcccc tttttgcagc
     5821 aaacgcccga gcgcactttg gtggacgtcg gtgtcacctt ttttatcgcc acgacggcgc
     5881 accagcggtg caacgccgag actgcaatgc caaaccgaac gcgtcagcgc cacgtaaagc
     5941 aaacgcagat cttccgccag acgttcgacc tccgcgaggt cgacgctttc tggcgcagca
     6001 ttaagatcca aaactgcctc aaacgagtgg cgatcgtgat aaaacgcctg atcctggacg
     6061 cggaaattgg taataaatgg caaccagacc agcggatatt ccagcccttt cgatttatgg
     6121 atcgtgacaa tctgcaccag atgtttatca ctttcgaggc gcatttgttg gctggaggca
     6181 ttactgtctg gctcgaggat atgttgcgat aaccagcgta ccagcgcatg ttcactttcc
     6241 agctgcgttc cggcttcttg aagcagttcg ctgatatgca agatatcggt aagacgccgc
     6301 tcaccgcctg ccgttgccag caagttttct gcaatgttac gcgccgacat cagcgcccgc
     6361 agcatcggca taacgccacg tttgcgccag atttgccgat aaccatcgaa ctcttcgact
     6421 accgcatccc acgcatgttc gtcattattc agcgtttcaa tatccagcgc attcagcccc
     6481 atcattgacg ttgccagcgc actgcgcagg gtgttctcac gttcgggcgt catcaccgcc
     6541 tgcaacagcc aaagcatttc ctgcgcttcc agagtttcaa aaacactatc gcggttcgaa
     6601 aggtaaacgg aagggatttc cagcaacgtt aaggcatcgc gcacctgggc ggcctcctgg
     6661 cggctgcgca ccagcacgct gatgtccgaa gcacgcaccg gacgcgcgtc gtcgccgttc
     6721 atcagcaacg cttcgccccg ctgtccggct tgtagccagt cgcggatttg cgcagcacat
     6781 acctgcgcca tggtactttg atagtcgcca acgccgcagc tttcgccttc catcagccac
     6841 attttcatcg caggctgcgt ttcaccttta aagacaaaac gtaacgcctg atttttcccg
     6901 gctgatttca ctggaataaa cggtatttcg cgaaacatga acgcgtcatc agtctggctg
     6961 aaaagcttgt tcacactgtt caccattcca ggtgcggagc gccagttggt gtctaaagtg
     7021 tagtgggcgt gaacttcact acgcgccttc atataagtga agatatccgc accccggaat
     7081 gcatatatgg cctgcttcgg gtcgccaatc agcaacaatg cggtttctgg ctgatggtgc
     7141 cagatacggc gaaaaattcg gtactgctgg gggtcggtat cctgaaattc atcgatcatt
     7201 gccaccggga atcgcgtacg gatcgccgct gccaacacct cgccgctttc gctacgcagc
     7261 gcggaatcga gccgacttaa catgtcatca aaacccaatt cgccacggcg gcgtttttca
     7321 cgcgctactg tttcgcggat ctcagccaat gcgcgggtga tcaccagatc acggatcgac
     7381 aatggttctg caagcagttg atcgatcgcc tcaaacagtg gatgtcgcgg ggtttccccc
     7441 ccggctttcg tgcgatcttc taagaaacgc tgggagaatt tttccagcga ctccggcaac
     7501 tgataactgt ttgtctcttc ttctgcccag gcgctgatct tgtcgatcca tttagcctga
     7561 ttgctacggt taaacttgcg tcgatcaata ccagaagatt cgatcagcgc atccagttca
     7621 cccactgcgt cgcgccactg ctgttttacc gtatcaatac gcgccacaat ttgcgcgtga
     7681 cgggaagcta gcgtttcatc atcgggcggc ggtgctttga taaccggcgc ttcgccttgc
     7741 agataacgat taatatcgcg cagcaacgcc tgcggccctt tccaggtttc aaagacgacc
     7801 tgggctattt cacgcggcag cgggtagcag tggcgacgcc agaaatcggc gcaggcctgg
     7861 tagcgtagca gagactcatc ttcaatcagc tgctgctcaa acagcatgcc ggattcaaag
     7921 gcattcaggt tgagcatgcg ctggcaaaag ccgtgaatag taaagactgc cgcttcatcc
     7981 atctgccgtt cggctaacaa caaccactgc gcggcttgcg ctttatcgtc gacctcttcc
     8041 agcaggtgtt cgtacaatgg attgtcggtg gtttcacgca gacaggcgat gcgcaactcg
     8101 tggatattgc tacggatacg accgcgcaat tctgccgtgg cagcctcggt aaaggtgacc
     8161 accagcagtt cttcaacggt cagcgggcgg ggaaaggcgg cggaaccgcc tagtccaagt
     8221 aacaggcgca aataaagcgc cgcaatcgta aaggttttgc ctgtgccggc agaggcttca
     8281 atcaggcgct cgccctgtaa gggcaagcgc aaaggatcta gtgtctcggc gacatcactc
     8341 attcttttca ctcatcaggg gcattgtttg ctgcaacgcg ctgacgttct cccacacttt
     8401 ccagccttca ggatgcacat attcggcttt cccgttttgg ctgccggaaa tctgcgacag
     8461 gatagccatg ccttgcggct cgactaccgc ctgatggaag aaatcggcca gtttttgcgg
     8521 cgtcagcagt ttaatctggg ccacgatttt atcacgcgaa tcgaagcgca tattgccgcg
     8581 atcgaaatct ttacttaact tcgatgcttc ttcgccgagc gtttgcggtg cctgcagcat
     8641 ctgggtaatt accgcctgct ggatttgcgc aaactcatct ggcttcatcg ctcgcaattt
     8701 tgcctctgcg gttgggaaaa acgccttgta acgctcccac aagaatgaag gctgtttatc
     8761 attgctttgc aaaaggaagc ccatgcccca ctgacgcccc acgctcattg gaaacgcaaa
     8821 cacggcatag cccaattgtt cttcggtacg caactgattg tagaaccacg gctgtacgat
     8881 ctgccccaac agagagctat aggctgagct ggtgtattca tcgtagccag tcggtacaaa
     8941 taccgctgcc agtgcggagt cggtgctgtt accggctttt tcaaagatga cggattgttt
     9001 tttatcgacc actacatctt tgtttcgaca ccactctgaa ccatcagcgc ccaactgttt
     9061 ttgcacatcg cgtgccagcg ttgttgcctg ggcctcggtc atgttgccga taaccataaa
     9121 ctctggtcga gcccctgatt ttaaggcgtc gcgataagcc agcacctctt tcaacgtaat
     9181 ggagggcaaa attttacgcc gttcatctcg cgagaagtac ggcacttgcg agagcatctg
     9241 cgcgggcata atcgcctgct caaacgcttt acccttttct gcggaatcca tcatctggtt
     9301 ataccaggac ttcgcctgct caagctgatc ttccgtagcg gtatagctaa agtacccctc
     9361 gagcaatgcc tggaacagct gcggcagacg ctgggtgtaa ccattagcat taaccataag
     9421 gccgttgtta gcgttggtgg aaaaacttat gccaccaacc gacgcctggt tgcttaactg
     9481 atcaagcgcc agccctgcga gataatcatt gagcgcaaac atcacctgat tgcgggcgct
     9541 gtccatggct ttcggattac gcaaaatcag gctgacatca gctttgggtt cgctggaaaa
     9601 ataacggctt ggcgcataca ccacgcgcag attcgactca tcaacaatca gctctggatg
     9661 gtcatatttc ttctctgact taatcagcga gaaatcgtca ggaatatagg ggttaagctc
     9721 cggtaaggag agcgcaatat tggcagcttt ttgctgccag tcagcgaaag tttgttcgct
     9781 gattttatcg acctgatacg gcgcatcgac aaaataagcc gttttattgt gcggctcttt
     9841 cgggctgata taccagatac gcgcattctg cggcgtcatc atcgccagac gttcttttac
     9901 tgctttagca tcgtaccgat cggcaatatt gactgcatcc agcgtatgct caaccggaac
     9961 gcgaatcatg gtatctgcca gccattcgac gtaatccata tcacgggtga tcgacggata
    10021 acggaagtcg atatccagca cattcgccag ttcatcgaag tattgtttat caatgccttt
    10081 ttcacgtaac agattgagat agctaaaaat tgccgccaca acctgatcgc gattagccag
    10141 gcctttatcg gttaaagacg cagagatcgc taatacgccg ctgttgccgt tgacgatagg
    10201 atcggagttg gcgctaatgc cctcaactaa tccctgcttt tgcagccagt cggaaagtgt
    10261 acctgggctg cgattaccaa tcagataggt aatcaattca tccgttttac tacggaactt
    10321 cgctgagttg ttatcgatgc gaaactcaac gcgcagaact ttacgcggca ttgccgggac
    10381 gtaatgaatg ataatgccct tttgcgcgtc ggtgactacc ggcacggtga tttccggttt
    10441 tttgctctct ttgttcggca cgcgaccaaa ggtgtccgcc gccatttttg ccaactccgg
    10501 cagcggttta ttgctgtaaa taaccgcctt catcagattg gcggaatagt acttctcgtg
    10561 gaaatctttc agcgcctgct gcaccggatt accaggttta tcgcttaaag tttcgaggtt
    10621 accaccagaa aactttgaac cggggtgtgc cgggttaatg gtttctgcgc tgacctgtgc
    10681 catgcgcatc ccgtcacgcg tacgcgccat ggttaattca gcgttcaccg cattacgctc
    10741 acgttcggca tatttcttgt cgagcaaagg ttcagcaata gcatcggcca ggcggtctac
    10801 cgcaccaagc aaggcgtcgt tctcaacttc cagatagaaa gccgtgcgat acggcgcagt
    10861 gctggcattg tgactaccgc cgtgcatttt gagatattcg gccagactgt cagcctgcgg
    10921 gtactttttc gaccccatca gactcatatg ttcaaggtaa tgtgccagcc cctggtacgc
    10981 ctcgggatct tccagcgacc caacgggcac caccagcgcc gagagcgatt taactgcctg
    11041 cggatcagaa accagcaaga ccaccatacc gttatccaga cgtatagcct gatactggcg
    11101 gttatcttta tcacttttac ggatggtttc ctgaatcggc tgccatcccg tttctgcctg
    11161 acttaagggt gcccaaaggg caactaacaa caataatgct ttgaaccagg tgctgcgggg
    11221 cattctcgga cctcataatc aacttaattt tctgtccaga ttcaacacgt taacgcaaat
    11281 catctgccag aatttaatct tgtgctgcac gagtcagcct atgtttatat aaccatcagt
    11341 ccgtgactgg tgcgcatcat aaagtaagcg gatagattgc gcaattttta tacagcactc
    11401 atgactgatt aaagcgaaac agcggtaaca ggaaacgttg cgactgttca acgatggcct
    11461 ccattgtctc tggtgttaat tgccgccaga gcctttgata ccagatatca tcaccttcgc
    11521 cacgcaccat catgttacct tcgtaagcct gaaggaattt cgtacgggct ttttgcaacg
    11581 tggaatcgtc atccagcatg gcatcgtttt gcgcgtcata acaggttttt agccacgcgc
    11641 cgccactttc aggtaacacc agcaatggcg cggacatccc ttcacgatac ccctcaatca
    11701 gttgtgagag gtaatgcaaa gcctgttcgg ctgcaagcgg cggaaaacgc cactcgccgt
    11761 ctttgcgtag aaaaaggcga ctttcaccat taccaccgct ggcacagtag acaaggtgtt
    11821 ccagccaaag ttgcattccc tgcgccacac ttaataaaga gggacgccag cgcaacaggc
    11881 catccggctg cacctgcggc aaccagccag ttatctgcac accgttgcag gcgagatcaa
    11941 tttccatact ctgccccggc tggcgacagg caatgactct gtcggcaagc tgctgcatct
    12001 cctggcactg agtttcccag aaaatttcac caaaagcgcc atacggtaaa tcccctgccg
    12061 cccggaagcg acggaacaag cgttcggcat catcctgctc aaccagtgca ttcaataact
    12121 gctgattgat ttgataacgg ctaagccctt ccagaataaa tggctcggtg tcggggattt
    12181 cgctgtcttc agtacggaag ttcacctgca aacgcatctg gaagaacgcc cgcaccggat
    12241 gtgcccagaa tcgttgtagc gtttccagcg gcacggtttc cggtaaggta aacggcagcg
    12301 gctgaacaaa ttcagaatgt gctttaccag cctggctggc cgcaggtagc cattcacgag
    12361 catagctttg tcgttcgcct ggctgatagt tttgtggatc aaacggcatt cgggtatgga
    12421 ggcaagtaag atgcgctttt acccttgcct cgctttcatc acagttgagc gcttcatcgc
    12481 ccggtagata atgactttgc ccgatgtagt cgatcagttc ctgcaccagt accgacggga
    12541 aacgttcact gttatcctga atggaacgcc cgatatagct gatatagagt ttttgctgcg
    12601 cggaaattaa cgcttccagg aacagatagc ggtcgtcatc gcgacggcta cggtcgccac
    12661 gcttcggttt ctggctcatc aggtcaaagc ccaatggcgc aagctgacgt ggataaacgc
    12721 cgtcgttcat tcccagcagg caaaccactt tgaacggaat tgaacgcatt ggcatcagag
    12781 tacaaatgtt aaccggtccg gcgagaaaac gctggctgat acgttcttga tccagacgct
    12841 gtgccagttc atcacgcaat agtgacagcg gcaccgcgtc gccatactgc gcacctaaac
    12901 cttcggcgat aatcgcctgc cattgttgtt cgatcagcgt catcgccgct tcggtttccg
    12961 catccggcag gaagaaggcg ctgagcatat cgcgacaaac cggcaaccac tcttctagcg
    13021 gacgctcctg tgccagcccg cgacgccaga tgtttagctg cattagcagt gaagccagat
    13081 gccccaccag ttctgcaatt aagccgctcg attcatcata aggtagaacc gattgccact
    13141 cgccctgcgc gctctccatc gcgtagccca gcaacatacg cgtcaggcca aatcgccagg
    13201 tgtgttgtcc ggtggcgggg agttccagct cgcgaacgtt gtcgtcatct atcccccaac
    13261 gaatgccgga ttcgttgacc cactggcgta aataacgcag cccttcttcg gtgatgtcaa
    13321 accgcgccgc cagcaccggc acatccagta atgccagcac gtcttccgac acaaagcggc
    13381 tgtctggcaa cgataacagg ctgataaacg cttccagtac cggatgcgac tgccgcgccc
    13441 gacggtcgga aatggcgtaa ggcaggtaac gatccgcagg tgcgctacca aacacagccc
    13501 gaataaacgg actgtagctg tcgatatcag ccaccatcac gatgatgtcg cgcggagtaa
    13561 gtgtcgggtc ttcttccagc atcgccagca ggcgatcgtg caaaacttca acttcacgct
    13621 gcgggctatg gcaaacgtgg aaggtgatac tgctatccag tggatcaagc gggcgtttgt
    13681 tatcgctacg ggaaaactct tcgatgttca caccagcaac ggcacggttt tccagttcca
    13741 gaatgtcaga ctgaatgtta tgcagcaggt tatctggcgt gacatcgaca aaggcgtcca
    13801 gctcctggct gctctccagg tcagaaagga gataaatgta gtcgcgccca agcttacccc
    13861 atgaagccag cagcgggttg ccgacatcct gttcaccatc gctgttaaag agctgcccgg
    13921 cattttcgct atcacgaaat agcggcaatt cgcgatcttc aaaactgtgt cgacgctgac
    13981 gggtcagcag tttcgccaga taagcaggat ctttaatatc gccccagtaa taacggcagg
    14041 ggttggtaaa caggagatgg atttcaatat gtttacccag cgcctgtagc gcctggagat
    14101 aaacaggcgg taacgcggaa ataccgcata taaagacgcg cgaaggtaac cccggcgggc
    14161 aggtcgtcgc ggactccagc gtttcgataa agcgctgata gagattggcg cggtgccagc
    14221 gcggttgccc gagttgatgg gtatattcca ccagcgcctt ccacaacggg gcttgccagg
    14281 cctgtgcttc tcccagccct tcaaccaaat gtcctgtttc ccactgtgcc agccagtccg
    14341 gacgatagac cagatactgg tcaaacaggt ccgccgcttt tgaggaaagc tggaacagtt
    14401 ttcgcttgtc gctatcgtca gtcagataat gccgcaacag ggtaaagtct tcgcgctcca
    14461 gcaattgcgg cagcagagtc atcagtttcc agctcatgct ctgtttgtta aaggcgctct
    14521 ctttggggat ttccggtaac acccggacga acatatccca gataaagctc gctggcagcg
    14581 gaaaatcaat gtttgccgca atactaaact tttgcgacag ggtcatttgc agccactgcg
    14641 ccataccggt actttgcacc agaatcatct ctggttcgaa aggatcgtcc agccgttcgc
    14701 gttcgacaat aaactccatc aacgcttcca gcacgtccag acgattggaa tggtagaccc
    14761 ttaacatagc ggctcctgac tactgacgat tcgggcaatg caggcgggtc atctcgcctt
    14821 ctctgccccc gggtgaaact agcgtaacgc tgatgctgac acatcccgcc tgcgatgtct
    14881 gcattcggtt gacctgccag ttggcaggtg gcgaaatcgc gcgcagttgc gtttgctgcc
    14941 aaccatgccg ccagagctgt tggtactggt ttctgctggc gagactgttc attaatgttc
    15001 gctggatacc cgataacgca gtgacaatca tcaccatcag caccatcgca accattacct
    15061 ccggcaggct aaagccttgt tgattcttca gggaagctga cataacgccc tctctttcag
    15121 cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatg tttccatcaa tgacttcgcc
    15181 tgttcgccac aacgaaacgc cttcataacc ggcaaccaat aaggcttcat tatctgccag
    15241 taaacgcaaa caaacccggg catcggttcc agcgtactgc gagcactgaa ctgcgggctg
    15301 cgtctgccag gagtgcattt tcccccacgc cagcgccgac tgaacgatgg cctggcggcg
    15361 caatgactga ctttctatgc tcacgcgaga ggcaaaactg cgatcctgct gactcattcc
    15421 ttgcaatagc aagctaccca aaaccagcag catcaggacc agagccagtg acgaaacacc
    15481 tttttcgcgg ttcacaggtt gaatcctgtc acgctatagc tggcatccac cacggtttgc
    15541 ggttcagact tactggcagc acgcatatta accgtcaaca ccggcgagaa gccgctgaca
    15601 tcctgacgta cgacctgaaa agtgtcgata atgatggcat ccggattagt gactttatcc
    15661 cagcccttac cttcacagga tgtcgcaccg cgtagcgttt ccagcacatg ctccttcaga
    15721 cgaaatccaa tctggtcaga ctcttttacc ggttcgcgat cccagatacc gttactgttc
    15781 gcatcccact gcacaatgat acagtcaccc tgtccgacaa tttccagccc ttcgccggta
    15841 cagctgccat gacaataacc cgccctctgg agatgcttcg cgacggtaaa tacccgcagc
    15901 cagatttcat cttccagcgc cagcttacgg gtgctcgtta aactttcacg ctgtaacgca
    15961 ggcagaaagc gtgcagcccc cagcaacaat acgctactga tcgccatagc aatcaacact
    16021 tccagcagag aaaaaccttg ctcttttaca ggcatccttc tgtttctcct tgctgacaaa
    16081 gccggagtct tccccacggc gaaaccacca gccaccactc gcccgttgag tttttgaagc
    16141 gaatatgccc ggcccatgcg gtattgcgca ggccaaagaa agcaagcgaa ggtgtcaggt
    16201 cgctcatttc gacttcgggc cagcgtggca caaagaccaa tggtgaactg ccatgacagg
    16261 tattggcccc agcagcggaa ctcacaaggc accataacgt cccctccctg ataacgctga
    16321 tactgtggtc gcggttatgc cagttggcat cttcacgtaa atagagcaaa tagtcccgcg
    16381 cctggctggc ggtttgccat agccgttgcg actgctgcca gtattgccag ccatagagtc
    16441 cacttgcgct tagcatgacc aaaatcagca tcgcgaccag cgtttcaatc agcgtataac
    16501 cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa aaaaagacga gggccagttt
    16561 ctatttcttc ggcgcatctt ccggactatt tacgccgttg caggacgttg caaaatttcg
    16621 ggaaggcgtc tcgaagaatt taacggaggg taaaaaaaac cgacgcacac tggcgtcggc
    16681 tctggcagga tgtttcgtaa ttagatagcc accggcgctt taatgcccgg atgtggatcg
    16741 tagccttcaa tctcaaagtc ttcgaaacgg tagtcgaaga tggattcggg tttacgtttg
    16801 ataatcaact tcggcagcgg acgcggttcg cggcttaatt gcagatgagt ttgatccata
    16861 tgattgctgt acaggtgcgt atcgccaccg gtccagacaa aatcacccac ttccagatcg
    16921 cactgctgcg ccatcatatg caccagtaac gcgtagctgg caatgttgaa cggcaggccg
    16981 aggaagacgt cacaggagcg ctgataaagc tggcaagaga gtttgccgtc tgccacatag
    17041 aactggaaga atgcatggca cggtgccagc gccattttat ccagttcgcc tacgttccac
    17101 gctgaaacaa taatgcggcg ggaatccggg tcgtttttca gctggttcag taccgtagtg
    17161 atctggtcaa tatgacgacc atctggcgtt ggccaggcgc gccactgttt accatacact
    17221 ggtccgaggt cgccgttttc atcggcccat tcgtcccaga tggtgacatt gttttcgtgt
    17281 agataagcaa tgttagtgtc gccctgcaga aaccacagca gttcgtggat gatggaacgc
    17341 aggtggcaac gtttagttgt caccagcggg aatccatcct gcaggttaaa acgcatctga
    17401 tgaccaaaaa tggaaagcgt tccggttccg gtacggtcgt ttttctgtgt gccttcgtcg
    17461 agcacttttt gcatcagttc taaatactgt ttcatggttc ctcaggaaac gtgttgctgt
    17521 gggctgcgac gatatgccca gaccatcatg atcacacccg cgacaatcat cgggatggaa
    17581 agaatttgcc ccatgctgat gtactgcacc caggcaccgg taaactgcgc gtcgggctgg
    17641 cggaaaaact caacaatgat gcgaaacgcg ccgtagccaa tcaggaacaa gccagagaca
    17701 gctcccattg ggcgcggttt acgaatatac aggttgagga taataaacag caccacacct
    17761 tccagcagca gctcgtaaag ctgtgatggg tggcgcggca gcacaccgta agtgtcgaaa
    17821 atggattgcc actgcgggtt ggtttgcagc agcaaaatat cttctgtacg ggagcctggg
    17881 aacagcatgg caaacgggaa gttcgggtca acgcggcccc acaattcacc gttaataaag
    17941 ttgcccagac gcccggcacc aagaccaaac ggaatgagtg gtgcgataaa atcagaaacc
    18001 tggaagaagg aacgtttagt acggcgggca aagataatca tcaccacgat aacgccaatc
    18061 aggccgccgt ggaaagacat gccgccgtcc cagacacgga acagatacag cggatcggcc
    18121 ataaactgcg ggaaattgta gaacagaaca taaccaatac gtcccccgag gaagacgccg
    18181 aggaagcccg catagagtaa gttttcaact tcatttttgg tccagccgct gcccggacga
    18241 ttcgcccgtc gtgttgccag ccacattgca aaaatgaaac ccaccagata catcaggccg
    18301 taccagtgaa gcgccacggg tcctattgag aaaatgaccg gatcaaactc cggaaaatgc
    18361 agatagctac tggtcatctg tcaccacaag ttcttgttat ttcgctgaaa gagaacagcg
    18421 attgaaatgc gcgccgcagg tttcaggcgc tccaaaggtg cgaataatag cacaagggga
    18481 cctggctggt tgccggatac cgttaaaaga tatgtatatg atccgcgcta taaccctccg
    18541 cgaatcagcc cgcccatgcc gcgacgctcc ataaaggctg caacctgatg gcgaacttcg
    18601 gtcgccagtt gcgcttccag actacgctgc gcaagatttt ctgcttcggc ataatcaatg
    18661 cgccgcagca ggtattttac ccgcgctaca gaacgtccgt tcatagacag atggcgatac
    18721 ccaagcccaa tgaggattgc cacgcacatg ggatcgcccg ccatttcacc gcacaaacgg
    18781 agatcgattc catgtatttc cgcttcccgg gcgatcatcg ccagagctcg taacattgca
    18841 ggatgaagac tgtcataaat gttcgccacc cgggtattgt tgcgatcaac ggccagaatg
    18901 tattgagtca gatcgttggt gccaacagag atgaaatcga cccgctttgc cagatgcggc
    18961 agcataaata ccattgacgg cacttccagc atgatgccga tacgtggttt gggaatttcg
    19021 taaccgatca tctcctcgac ttcacgtccg gcacgttcaa tcaggcggcg tgcttcatca
    19081 acttcatcga ggcttgtgac catcggcaac agaatattca ggttgcccgt agcggcatta
    19141 gcacgcagca tcgcccgcac ctggatcaag aagatctccg gctgatcgag cgtaatgcga
    19201 atcccacgcc aacccaggca tggattctct tcgctgatcg gcatgtaagg cagctgctta
    19261 tctgctccga catccagcgt acgcaaggtg acgggtttat cattgaacat ttgcagcatc
    19321 ccctgatact gcgccacctg ttcttcttcc gacgggaaac cactttgcag catgaatggg
    19381 atttcagtgc gataaagacc gatgccatca atacggctgc ccagtttttc ttcatgttcc
    19441 gggcttaaac cagcattgag catgactttt atacgctcac cgctttttaa ctgcgcgggt
    19501 aaattgacgt catcttccgc cagacggcta agctcaatct cttcactaat tagccgctga
    19561 tattcttgca gcagtaccgg ctccggatcg accagcaatt caccgcgata gccatcaacg
    19621 atcagcgtcc tacgatgcag caccgaaggc tgaatatccg cgcccatcac ggtagggatc
    19681 cccagcgcac gtaccatgat cgcagcatgg gagttggctg cgccatctcg cacgacaaca
    19741 ccgactaagc gatcctgggg cagctcagca agagttgtcg ctgacagttc atctgccacc
    19801 agaatgaaac gttccggcca ggcgttcggc ccttgattag cgtcatcaag atgaaacagc
    19861 aatcgctgac ccagcgcacg taaatcgcca gcccgctctt tgagatagtt atcgcttagc
    19921 gcggcaaact gttcggcaaa tttttcaatg accgttttta ccgcccactc tgccaccgag
    19981 cctttatcaa cctcggcaaa caattcgcga cgcagccggg tgtccgaaag taggtgcgag
    20041 taaagatcga aaatagccgc cgtttctttt tgtgctccgg cggcaaagcg tttgctgtag
    20101 cggcgaaact cgtttgccgc ttcttccagc gccccggtca gtcgttcgcg ttccagagcc
    20161 ggatccagcg ttgatgcctg atacacctgt tccattaaag gtaacgtggc atcctgccag
    20221 ccttcggcaa tcgccacacc aggtgctgcc ggtaatgcgc ggattcgcgt ctggcgatat
    20281 tgcccaaaca aggcagtcaa ctgcgactga gaaagaatag ctgccatctg ggtggcaagc
    20341 gtcaccagga aggattcttc actttcgtca tactggcgca actctcgttg ctgtaccacc
    20401 agtacaccaa gcaactggcg acgttgaata attggtacgc ctaaaaacgc gcggaaacgt
    20461 tcttctttta cggaggggat gtatttgaag ctggggtgct tttgcgcatc tgcaaggttt
    20521 atcggttccg ccagcctgcc aaccaggccg acgatccctt catcaaacgc gagcgttaca
    20581 gtgcgaccgc gtggtttttt cagcccccgg gtcgccatca ggtagtaaca acgtcgatca
    20641 tgatcggcca ggtagaccga acagacctcg gtatccatcg caagacagat gtcggtaacc
    20701 agaatattta acgcctcatt caggcgtggt gcgctggcta ccttttcgac tatttcgcgc
    20761 aggcgagtga gcataattgg cgtgacttaa cctcttttac gtcgataagc agatgtgttt
    20821 tgtggttttg gcgtattttc ctgcagtgac atcaccacac tcgcgaactc tttcattacc
    20881 ctacggtaga catcacgttt aaatgacacc acctgtctga ccggatacca gtaacttacc
    20941 catcgccagc cgtcaaactc tggtgtactg ctggtttgca tattgatttc tgcatcgccg
    21001 ctcaccagct gcaagagaaa ccatttttgt ttttggccga tacaaaccgg cttcgtgtcc
    21061 caacgcacca aacgtttcgg taatttgtag cgcaaccagt tacgcgttga agcaaggatt
    21121 cgaacgtctt tgcggcttaa tcctacttct tcaaacaatt cacggtacat cgcctgctct
    21181 gcggattctc cggggttgat tccgccttgc ggaaattgcc aggagtgctg accaaatcgc
    21241 cgggcccaca ttacctgccc ctggcgatta caaatcacga tacctacgtt tgggcggtag
    21301 ccatcgtcat caatcaccgg actacctcaa aataaagctt tatatacgaa tgattgtttc
    21361 atactccaag aagacggtaa accactctct gcagggcatt acacactaat aacaattgaa
    21421 taactcacag ttatgtgcag agttataaac agaggaaggg gtggatagcc gtttttattc
    21481 accttttctg tggatagagt tgtgaagaac tacggaatta ctacgggaaa acccggagca
    21541 ttctgaataa gcccgatata gacattttaa aatattcatt attagtcaat gcattacatg
    21601 taatttgagt aatgaaaaag agtaattcgt gacccaggtc acacctctca tttacgggtt
    21661 ggcgaaagat caaccaatgc cgtatttatc cacagaatgt gccactaagt taagcactga
    21721 accactaaaa actggagttt cgtcgcacgt caaggctgta aatggaaaca gtagtggagg
    21781 tttttcacag ttatcccagc tttctgtgga taacatggtg taagatcctg tttattttca
    21841 gtgaccagat ttggaaaacc tattgcagtg ttgcgcaact cgattaccgg cagcctaaaa
    21901 agcaatataa atcagagaat tgaacaacac atgtggaaaa agttacactg cgaatattcg
    21961 gcacataatt gctgtttgtt ttttaaccaa ggtatcatga catgtcccaa cctcgcccac
    22021 tcctctctcc tcccgaaact gaagaacagt tgttagcgca agcacagcaa ctttctggtt
    22081 atacattggg agaactggcg gcacttgccg ggctggttac gccagagaat ttaaaacgcg
    22141 ataagggctg gattggcgtg ttactggaga tctggctagg tgccagcgca gggagtaaac
    22201 ctgagcaaga ttttgctgct ctgggcgtgg aacttaaaac tatccctgtg gatagtcttg
    22261 gtcgtccgct ggaaacaaca ttcgtttgtg ttgccccgtt aacgggtaat agcggggtga
    22321 cctgggaaac cagccatgtg cgccacaagc tcaaacgcgt actgtggata ccggttgaag
    22381 gtgagcgcag catcccgctg gcgcagcgtc gcgtaggatc accgttgctg tggagcccga
    22441 atgaagagga agaccgacag ctacgcgaag actgggaaga attaatggat atgattgttc
    22501 tcggtcaggt tgagcggatc accgctcgtc acggggagta tttacagata cgaccgaaag
    22561 cagcgaatgc gaaagcgctt accgaagcca ttggtgcccg gggcgaacgg attctgacgc
    22621 tgccacgcgg cttttatttg aagaagaatt tcaccagtgc actactggcc cgtcattttc
    22681 tgatccagta gccatcgctt tgacctgccg ctttccgggc atataattac cgcttcattt
    22741 ttttggcagg gctttttaga tgttatttgc atggataacc gatcctaacg cctggcttgc
    22801 gctcggtacg ctgacgctgc tggagatcgt tcttgggatc gacaatatta ttttcctttc
    22861 tctggtggtg gcaaagctcc ccacagcaca acgtgctcat gcgcgccgtc tggggttggc
    22921 gggagccatg gttatgcgtc tggcgctgct ggcatcaatc gcctgggtta cgcgcctgac
    22981 aaatccgctt tttacaatat tcagtcagga aatttccgcc cgtgatttga ttctgcttct
    23041 gggtggcttg ttccttatct ggaaagccag caaggaaatc cacgaatcca ttgaaggtga
    23101 agaagaaggg ctgaaaacac gcgtttcatc attcctcggc gctatcgtgc agattatgct
    23161 gctggatatt atcttcagcc tcgactcggt gattaccgct gtgggtctgt cagatcacct
    23221 gtttattatg atggccgccg tggtgattgc cgtaggcgtg atgatgttcg ccgcgcgctc
    23281 gattggtgat tttgtcgaac gccatccttc ggtaaaaatg ctggcgctct ctttcctgat
    23341 tctggtcggc tttaccctga ttctggaaag tttcgacatc cacgtaccga aaggttacat
    23401 ctacttcgcg atgttcttct ctattgcggt tgaaagcctc aacctgattc gcaacaaaaa
    23461 gaacccgctc tgatactccg tacgctctcc tgcgggaggg cgtactcact cctgttaccc
    23521 tcacagttta agattttcct gctttcaaga tatataacgt cggtttataa acagactatt
    23581 atcataggtg ggccaaatga ggataaccgg atgaaaaaat gggcagtaat aatttctgca
    23641 gtcggactgg cgtttgctgt ttccgggtgt tccagtgatt acgtcatggc gaccaaagat
    23701 ggccgtatga ttttgaccga tggaaaacct gaaattgatg atgataccgg gctggtgagt
    23761 tatcacgatc agcaaggtaa cgcgatgcaa attaaccgtg atgatgtttc gcaaattatt
    23821 gaacgttaac aaataaggtc agcatccggc tgtaatgccg atcagttaag
//
LOCUS       DAJTYZ010000061        23377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000061 DAJTYZ010000000
VERSION     DAJTYZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.061"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="OO596_002254"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="OO596_002254"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6718101.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="OO596_002255"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="OO596_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6718102.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="OO596_002256"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="OO596_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6718103.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="OO596_002257"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="OO596_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6718104.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="OO596_002258"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="OO596_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6718105.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="OO596_002259"
     CDS             5280..5738
                     /locus_tag="OO596_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6718106.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="OO596_002260"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="OO596_002260"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6718107.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="OO596_002261"
     tRNA            6790..6874
                     /locus_tag="OO596_002261"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="OO596_002262"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="OO596_002262"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OO596_002263"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OO596_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6718108.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OO596_002264"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OO596_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6718109.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OO596_002265"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OO596_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6718110.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="OO596_002266"
     CDS             complement(10156..13899)
                     /locus_tag="OO596_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6718111.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OO596_002267"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OO596_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6718112.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="OO596_002268"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="OO596_002268"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6718113.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="OO596_002269"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="OO596_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6718114.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OO596_002270"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OO596_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6718115.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="OO596_002271"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="OO596_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6718116.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OO596_002272"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OO596_002272"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6718117.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OO596_002273"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OO596_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6718118.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="OO596_002274"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="OO596_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6718119.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..>23377
                     /locus_tag="OO596_002275"
     CDS             23294..>23377
                     /locus_tag="OO596_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6718120.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIV"
BASE COUNT         5859 a         6116 c         5627 g         5775 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcgacc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc aatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtc
//
LOCUS       DAJTYZ010000062        23215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000062 DAJTYZ010000000
VERSION     DAJTYZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.062"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(28..228)
                     /gene="glgS"
                     /locus_tag="OO596_002276"
     CDS             complement(28..228)
                     /gene="glgS"
                     /locus_tag="OO596_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6718121.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            497..1126
                     /locus_tag="OO596_002277"
     CDS             497..1126
                     /locus_tag="OO596_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCR6718122.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1153..2814
                     /locus_tag="OO596_002278"
     CDS             1153..2814
                     /locus_tag="OO596_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR6718123.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3053..3112
                     /locus_tag="OO596_002279"
     CDS             3053..3112
                     /locus_tag="OO596_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6718124.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3429..3488
                     /gene="ibsE"
                     /locus_tag="OO596_002280"
     CDS             3429..3488
                     /gene="ibsE"
                     /locus_tag="OO596_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR6718125.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3608..5041)
                     /gene="hldE"
                     /locus_tag="OO596_002281"
     CDS             complement(3608..5041)
                     /gene="hldE"
                     /locus_tag="OO596_002281"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6718126.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5089..7929)
                     /gene="glnE"
                     /locus_tag="OO596_002282"
     CDS             complement(5089..7929)
                     /gene="glnE"
                     /locus_tag="OO596_002282"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6718127.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7952..9253)
                     /gene="ygiF"
                     /locus_tag="OO596_002283"
     CDS             complement(7952..9253)
                     /gene="ygiF"
                     /locus_tag="OO596_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6718128.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9495..10115
                     /locus_tag="OO596_002284"
     CDS             9495..10115
                     /locus_tag="OO596_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6718129.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10179..11417
                     /gene="cca"
                     /locus_tag="OO596_002285"
     CDS             10179..11417
                     /gene="cca"
                     /locus_tag="OO596_002285"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6718130.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11580..12401)
                     /gene="bacA"
                     /locus_tag="OO596_002286"
     CDS             complement(11580..12401)
                     /gene="bacA"
                     /locus_tag="OO596_002286"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6718131.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12492..12860)
                     /gene="folB"
                     /locus_tag="OO596_002287"
     CDS             complement(12492..12860)
                     /gene="folB"
                     /locus_tag="OO596_002287"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6718132.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12965..13582
                     /gene="plsY"
                     /locus_tag="OO596_002288"
     CDS             12965..13582
                     /gene="plsY"
                     /locus_tag="OO596_002288"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6718133.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13595..14527)
                     /gene="ttdR"
                     /locus_tag="OO596_002289"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13595..14527)
                     /gene="ttdR"
                     /locus_tag="OO596_002289"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14734..15645
                     /gene="ttdA"
                     /locus_tag="OO596_002290"
     CDS             14734..15645
                     /gene="ttdA"
                     /locus_tag="OO596_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR6718134.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15642..16247
                     /gene="ttdB"
                     /locus_tag="OO596_002291"
     CDS             15642..16247
                     /gene="ttdB"
                     /locus_tag="OO596_002291"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6718135.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16296..17759
                     /gene="ttdT"
                     /locus_tag="OO596_002292"
     CDS             16296..17759
                     /gene="ttdT"
                     /locus_tag="OO596_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6718136.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17802..18815)
                     /gene="tsaD"
                     /locus_tag="OO596_002293"
     CDS             complement(17802..18815)
                     /gene="tsaD"
                     /locus_tag="OO596_002293"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6718137.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19052..19267
                     /gene="rpsU"
                     /locus_tag="OO596_002294"
     CDS             19052..19267
                     /gene="rpsU"
                     /locus_tag="OO596_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6718138.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19378..21123
                     /gene="dnaG"
                     /locus_tag="OO596_002295"
     CDS             19378..21123
                     /gene="dnaG"
                     /locus_tag="OO596_002295"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6718139.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21318..23159
                     /gene="rpoD"
                     /locus_tag="OO596_002296"
     CDS             21318..23159
                     /gene="rpoD"
                     /locus_tag="OO596_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6718140.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5645 a         6325 c         5905 g         5340 t
ORIGIN      
        1 ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc ttgcctgcat
       61 catctgttgg caataccagg cataacgtgc gcaaaaccag gttctatgtt cttcatccat
      121 gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc tggcaaaact
      181 acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa gcacatcctc
      241 cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt ccccaatcag
      301 tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc ccgatcacac tttttagtga
      361 aaaataaaaa actatattaa aaaactttct cttccagaac ttctctaaca tgtgttatta
      421 ttaacaaaga taaatgtaag caaggaaact atcccaaagt aaatattgcg tcactaaatg
      481 gacattggag tgacatatga ttttattcgc cgactataat accccttatc ttttcgctat
      541 ttcctttgta ctgttgatcg gccttttaga aattttagcg cttatctgcg gtcacatgct
      601 ctccggtgct cttgatgcac atcttgacca ttataattcc ataaccacag gtcatattag
      661 ccaggcactt cattatctca atatcggaag attgccagct ctcgtcgttc tctgcttgct
      721 ggctggtttt tttggtctta ttggcatcct gttgcagcat gcctgcatca tggtctggca
      781 atcaccactg tcgaacctgt tcgtggttcc tgtcagttta ctgtttacga ttattgcggt
      841 gcattatacc ggtaaaatag ttgcgccctg gattccacgc gaccatagtt cagctattac
      901 agaagaagaa tatattggta gcatggcatt aattaccggt catcaagcca cttcaggtaa
      961 tccctgcgaa ggaaaactca ccgatcaatt tggccaaata cattatttac tactggagcc
     1021 tgaagaagga aaaattttca ctaaaggagt caaagtgtta attatttgcc gactttctgc
     1081 aacgcgatat ttggcggaaa acaatccctg gccacaaata ttgtaattta ttaatcaaaa
     1141 ggaaatttaa taatggatga tattgttaat tctgtgccct cctggatgtt taccgcgatt
     1201 attgccgtat gcattctgtt tattattgga attattttcg ccaggctcta tcgtcgcgct
     1261 tcggcagagc aagcttttgt tcgtactggt ttaggtgggc aaaaagtggt gatgagcggt
     1321 ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca tcaatatgaa tactctgaag
     1381 ctggaagtca gccgctcaac cattgatagc ctgattacga aagatcgtat gcgtgtcgat
     1441 gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag aaggcatcgc cactgcggcg
     1501 cagacgttgg ggcaacgcac gctatcacca gaagacttac gtatgttggt tgaagataaa
     1561 tttgtcgatg ccctccgtgc aacagctgca caaatgacca tgcatgagtt acaggatacc
     1621 cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag aagacctatc gaaaaacggt
     1681 cttgagctgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga acatttcaat
     1741 ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac ggagcgccgt
     1801 cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg cggtgcgtga gaaaaaccgt
     1861 gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac gcttgagcag
     1921 gagcagcagg ttaaaacccg tactgccgaa cagaatgcac gtattgcggc ttttgaagct
     1981 gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacaaat tcaggaaacg
     2041 gagatcgagc gcgagcaggc cgtccgctca agaaaggttg aggctgaacg tgaagttcgt
     2101 attaaagaga tcgaacagca gcaggtcacc gaaatcgcca accagacgaa atcgatcgct
     2161 attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct tgcactcgca
     2221 gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc agaccgcaga agccgatcgt
     2281 gctaaacaag ttgccctaat cgctgccgcg caggatgcag aaaccaaagc ggttgaactg
     2341 accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc aggcggcggc tatcgttgag
     2401 ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg tgcgctgaac
     2461 gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact ggccttgttg
     2521 caggcgctac ctgcggtaat agagaaatcc gttgagccga tgaagtccat tgacggtatc
     2581 aagattattc aggtcgatgg tctgaatcgt ggcagcgctg cgggtgatgc aaacacgggt
     2641 aatgtggggg gcggaaacct agcggaacaa gcattatcag ccgctctctc ttaccgcaca
     2701 caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg ctcactggcg
     2761 gcattgactt cacccttaac ctcaacaact cccgtcgccg aaaacgtaga ataaattttc
     2821 agctatgaac gtccctggca caaaaagccg gggatttttt atatctgcgt tccgctaaaa
     2881 actgcaaatg atcaggccgt tgcaacgttt tgcgtgaccg ctcggggaag gcaaaactgc
     2941 ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca atcggggagt cagcaaaggg
     3001 ctgaaacggg aaagcccctc ccgaggaagg ggccttacag aaggaaaggg ttatgatgaa
     3061 gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt aacagccaat
     3121 cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga aaaaaaatca
     3181 gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa aagccgggga ttttttatat
     3241 ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca gcgttttgcg tgatcgctcg
     3301 gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca tcaacaatcg
     3361 gggagtcagc aaggggctga aacgggaaag cccctcccga ggaaggggcc ttatagaagg
     3421 aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta agtttcccga
     3481 cttactaaca actgatcaga gggggaagaa atcctccctt acccttgttc ctttacttta
     3541 ggttgaaaaa acaacagcgt caataggcct gcctgctaca aagcgagatc tgtgaaccgc
     3601 tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttagtg gtcgagcaac
     3661 cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc ccagacttct ttactcccgg
     3721 cgatctcttc tggtttatag tcgccgccct tcaccagtaa atctggcagg atcccggcga
     3781 tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca gtcgaccgct tccagtgcgc
     3841 ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct ttcagccgtt
     3901 tggtggaggc atcgctgttc acagcgacaa tcagacggtc accctgtttg cgagcatttg
     3961 ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc attaccacct
     4021 tttcgccgcg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg gtcatcacgc
     4081 caaagccggt atctgcgcgc ccacgcactg cattttccag ctcgatcggc gaaaccgtgg
     4141 aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag caggcttctt
     4201 ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg tcgcccgcac
     4261 cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct ttacccggtt
     4321 gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt tcgtaatcgg
     4381 caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta ccgacaacag
     4441 cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc tcaaaatcgg
     4501 taccttttgg atcaatcagc accggaacgc ccgctttacg cgccagttgg atcatctgct
     4561 gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca atcgaactca
     4621 gcgcctgatt aatccgctcg tgcagcggct gcggatcaac gccttcgaaa ccttcttcga
     4681 aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggtg atggtcggat
     4741 gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc ggccagagat ttactcagcg
     4801 cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca ccgagagaag
     4861 cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta ttcactttaa
     4921 ccacgggcac cggcgcttcc ggcgagatac ggctggaggg accgtaccag taacgatcca
     4981 gcattacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc gttactttca
     5041 ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc attcttccac
     5101 cagccacttc tgccagcttg cccgcaccag atcacgctct gcggtgaagc aatcccccga
     5161 cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgca gcgtcgtgta
     5221 agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg ccagcagctc
     5281 cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag cgtagcgcaa
     5341 caccagatat tgggtaataa attcgatatc ggtaattccg ccttcatcag ctttgatatc
     5401 aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca tttcccgcac
     5461 ttccgtttgc agagttctac cttcacgcgg cagcgtcata atctcgcgac gcactgcgtc
     5521 aaagtgcgcg gtgagctgcg gatcgccgta cactacccgc gcacgcacca gcgcctgatg
     5581 ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgtgg aggtaaccag
     5641 cattcccgcc gccccggacg ggcgcagccg cgcatccact tcgtacaaaa tgccggaaga
     5701 ggttcgcgtg ctgaacaggt gcataatgcg ttgcgccaga cgaagataaa actgtcgtcc
     5761 gtcgatttcc cgctcgccgt cagtcatcgc atccattggg caatcatgga ggaagatcag
     5821 gtcaagatcg gaactgtagc ctaactccca gccgcccagc ttgccgtagc cgaccactgc
     5881 aaaaccacgc ccttcgcgat cgttgaggtg attcggctta ccgtagcggg caaccatttg
     5941 aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg ttaagtgatc
     6001 gctcactttc atcaccggta gcgtaccagc gatatccgct gcggcgatgc gtaacagctg
     6061 cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt ccggcacgcg
     6121 cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct ggtaaagagt
     6181 gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc tggcaatcat
     6241 cggcgatgcg gcacacagag aaatcaaatg gttaagcgcc gcagggaatt cactgagcaa
     6301 ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc gcgacagcgt
     6361 gacagcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca gatggtcgag
     6421 cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga aatcggaaat
     6481 cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg gcgtagtgtc
     6541 atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg actcttcctg
     6601 agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat tgttcatatg
     6661 tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc acgccagccg
     6721 cgcacggtta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa tgctttgcag
     6781 caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag catcgttttc
     6841 agaaagcaga tgcagcgcag caatggcgct gagcgttggc agtaaagagc acgattgcag
     6901 cgacggttcg cgcccgccgc gaatgagctg gaatacctgg acgataaatt caatttcgcg
     6961 aataccaccc gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca cttcacgggc
     7021 aatcattcct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt aacgacggaa
     7081 aacaaacggg cgtagcatcg cacgcaactc gttagcatag acgccttcgc tatcgcccat
     7141 aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct ggtaataatc
     7201 ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac gcagtcgcat
     7261 atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa tcagccgctg
     7321 ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct gcgtacaacc
     7381 atgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt ccccgccgcc
     7441 cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat tgcacggcgt
     7501 tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa tcagcgtctc
     7561 cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca gcgtttgcgc
     7621 ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca ttaacccggc
     7681 ttcgtcactc acattactga gcgcctcctg caaccatgca gcgtaatgct gccattcatc
     7741 ggcctgcggc ggttggcttt ccagttccgt caaccactct cgatgcgcaa ttatgctgtc
     7801 ctgcacaaaa tcactgaaag taagtactga cttcgcctgt gcacttagtg gttcctcggc
     7861 taaaggctct ggcagccgct caacaacggt ctgcctgtac tgctgtaacg gtgaagagag
     7921 cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc aaccagaacg
     7981 gttcctgatt gtttgcctca ttacggaaat gctcaatttc aatgcgttgc ccggtcgcaa
     8041 tagcgtgacg cagcccctgc cagttctcca gccaggcatg cgcgacgaca ggatcatagt
     8101 aacccgccag caacagtatt gagtcaatgt cacgcgtcag gcgtggcaac tggtcacggt
     8161 agagatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg tgggaaagat
     8221 ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg gcatctaaaa
     8281 atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc attgccgttt
     8341 cggtagagta gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct tcgcattggg
     8401 tcagcaaatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg aacagcatca
     8461 gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca tcgttaccgc
     8521 gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc gccgcttcca
     8581 gcccctgttc tacatcggct tttgccgcaa catgcaaaat ggtggtcggt ttgatttcac
     8641 gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg ctgccctggc
     8701 gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc gtgtcgccgc
     8761 taagcagttc cagttccagc tcacagatag gttcagcaaa ttcccccgct ttcacctccc
     8821 cctgatcgag agcgatttca attcggctgc catcgaccgc caccagccat ttttcgcgat
     8881 aaaaatcggt gctgaacagc ggttgtacgc gggaagcgag atcggcaggc aattcaccgt
     8941 tcggccagac ttccgaggga aactgcgcca ggtcgagcgt cggttcgctc aacgccacgt
     9001 tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc atggtcatct
     9061 catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc agccagttat
     9121 ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg ccgcccagcg
     9181 tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac tttaattcga
     9241 tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat gaacggcgaa
     9301 cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca tgcaacgggc
     9361 aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg attccgattg
     9421 atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact ttccatgaca
     9481 ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc gcacttagcg
     9541 cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg aatacctggg
     9601 tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc ggcgaggaag
     9661 tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac agctctggcc
     9721 gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc tcccgtgtgc
     9781 cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc gataacacct
     9841 ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc gtgatcaacg
     9901 ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag aaaaaggtcg
     9961 atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa tggtttatgt
    10021 atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg cacctgatcc
    10081 caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt gcactgtctt
    10141 attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat ctggtcggtg
    10201 gtgctattcg ggatgcattg ttagggctac cggtcaaaga cagagattgg gtggtagtcg
    10261 gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc gattttcctg
    10321 tgtttctaca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa cggaaatccg
    10381 gttccggtta caccgatttt acttgctatg ccgcaccgga tgtcacgctg gaagatgatc
    10441 ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc gagattatcg
    10501 acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt tcccccgctt
    10561 ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt tatgcccacc
    10621 tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc catgcgggtg
    10681 aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc ctcaccaccc
    10741 gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc gttttattcc
    10801 cggaaattga cgcactgttt ggcgttccgg cccctgccag gtggcatccg gaaatcgaca
    10861 cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg caggtcgatg
    10921 tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca gaactttggc
    10981 cgcgtcatca tggtcacggt ccggcgggtg tgaaattagt ggaacaactc tgccagcgtc
    11041 tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt cacgatctca
    11101 tccacacctt cccaatgctg aacccaaaaa ctatcgtcaa attgtttgat tccatcgacg
    11161 cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct gacgtacgcg
    11221 gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc gaagcctggg
    11281 aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa ggtgtggaga
    11341 ttcgcgagga gctgacccga cggcggattg cggcggtagc cagttggaag gaacaacgtt
    11401 gcccaaagcc tgactgagta taaaactaaa cgttaacacg aaccatcccc tcgccccttc
    11461 ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc acccccaccc
    11521 cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc ttttctcaat
    11581 caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc gaacgggata
    11641 aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc caccacaaaa
    11701 gcggtgataa acccaacggc aaacatcgag atatcgccgg ttgtcaggaa gccccagctt
    11761 ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag gaacgagaac
    11821 tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt ggccccggaa
    11881 cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa cgcctgacga
    11941 taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca ttcggcggca
    12001 atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt aaacaatgac
    12061 ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc cagcaaaatg
    12121 tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg gccaaagtgg
    12181 atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag aattgatcct
    12241 aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa caagtgaccg
    12301 acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc ttcgaccaca
    12361 cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc cctaaattat
    12421 taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg tttggtttaa
    12481 cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat tacaccaaca
    12541 ttcgccgccc gcgccattgc gcctggcttg ctgagtttga tgcgcaccca cggcgagttg
    12601 aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag cgcaaaacgc
    12661 gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag gcaatccgcc
    12721 acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc gaacactaac
    12781 ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac cgaaagttgc
    12841 tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga taccacttcc
    12901 ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca aaacggaaca
    12961 gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg gctccatttc
    13021 cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca gcggctccgg
    13081 caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg tagcagtact
    13141 gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat taggtgtcag
    13201 ccccttctgg ttaggcttaa ttgccatcgc cgcctgtctt ggacacatct ggcccgtttt
    13261 cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg cacccattgg
    13321 ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat tgagcggata
    13381 ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct ggtggtttaa
    13441 gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc gtcatcatga
    13501 caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat tcaaaagaaa
    13561 gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag ccttcatcgg
    13621 gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca cgtaatttca
    13681 tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt gcatactccg
    13741 gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac tcggaacgca
    13801 gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta ttggaggaaa
    13861 gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc catattccgt
    13921 gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc tgtaatgatt
    13981 gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt ttatttttcg
    14041 ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata tccagatcaa
    14101 tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc tgtaactcag
    14161 gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca aaaccaaaac
    14221 tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttacgtgacg tcatcgacca
    14281 gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg ccactttcag
    14341 tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta ttttcaagga
    14401 tttggatccg cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct gcggcgctaa
    14461 aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa ggccagctat
    14521 tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa aacattgcca
    14581 ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa ttgttaatta
    14641 catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg tggcggtgaa
    14701 tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa taagcaacag
    14761 gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc tacccgaatg
    14821 cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc gtcgatgggg
    14881 aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct gaatcgtcct
    14941 gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg cttcccactg
    15001 cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt caaagcgcca
    15061 ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa taccggtagc
    15121 ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga aatcgaagtt
    15181 tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat gccgtcagaa
    15241 ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc cgtaaacgcc
    15301 tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc cgccgtactc
    15361 tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa agcggcagaa
    15421 ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca agggctgact
    15481 ggcaacagtt cagtgatggg cgtacatatc gaatctgccg cccgccatcc gtcaaccatc
    15541 ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacactgct ggttcatgcc
    15601 gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag atcctgacaa
    15661 ccccgatcaa agctgaagat ctgcaagata ttcgcgtcga cgatgtgatc tacctgaccg
    15721 gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg aaacgtccga
    15781 tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg cgcaaaaacg
    15841 gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg gaaagttttg
    15901 aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc ggaatggggc
    15961 cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc ccggcaggct
    16021 gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg acagagctcg
    16081 gaatgccgga gtcgctgtgg gtctgtcggg tcaaagagtt cggcccgctg attgtctcta
    16141 ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc gaacgccgcg
    16201 atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc tcccggagag
    16261 gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact gaatggtggc
    16321 gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt cccgcagggc
    16381 tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt ggactgatcc
    16441 tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat atccatcatc gccatactct
    16501 ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa ttcactgcaa
    16561 aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg attttcgccg
    16621 cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt gcgctgattc
    16681 tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg ttctccgagc
    16741 tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt atctatccca
    16801 tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt tcgcgcagca
    16861 ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc gccattttct
    16921 tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca tctcacgcca
    16981 cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg ctggtgctgc
    17041 tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc gatcaggtgc
    17101 cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt gaaaaacgga
    17161 tgctggggct gatagtaggc gcgctggtgc tgtggatttt cggcggtgat tatatcgatg
    17221 ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt atcagttggg
    17281 acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc tcgcttatca
    17341 ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa ctgttagcag
    17401 gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg gtgttttatc
    17461 tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca ccgatgatga
    17521 ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg atggttggcg
    17581 cggcaattgg tctggggagc attcttacac catacgccac cggacccagt ccgatttact
    17641 acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt tttgggctga
    17701 tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg ttgctttaag
    17761 tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc cggtaactcc
    17821 gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc tgctttaaac
    17881 cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaactc cggacgcgcg
    17941 tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg taacgtgcgg
    18001 ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc caacgctcgc
    18061 ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc gatatcagca
    18121 cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt tttcagacca
    18181 gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac aaagcgcccg
    18241 gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc cagccccagc
    18301 agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc gccgagcagc
    18361 tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga caccagcagc
    18421 gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag atgcccttcc
    18481 atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg ccccacggtt
    18541 gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac agcatcaatg
    18601 tcttttgccg ttaagccaga ctcctttagc gccgcctgga tcaacggtac ggttttgcgc
    18661 acgtgatcgc gggaggccag ttcaggcacg acgccgccat agtcagcgtg caatttcacc
    18721 tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc gatgccggtt
    18781 tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc gctttattac
    18841 cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg gagaaagcct
    18901 cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa ggccgcggtg ctttacaaag
    18961 cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt gatgccagcg
    19021 gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac gtgaaaacga
    19081 gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag gtgttctggc
    19141 ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc gcgctaaagc
    19201 ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc gcactcgtct
    19261 gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg taaggccgtg
    19321 cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc ggggcctatg
    19381 gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga catcgtcgat
    19441 ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc gtgttgtcca
    19501 ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt ttaccactgc
    19561 tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga caagctcgag
    19621 ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc atttgaagca
    19681 ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca gttgatggac
    19741 ggtctgaata cgttttacca acaatcttta caacaacctg ttgccacgtc tgcgcgccag
    19801 tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat tggttttgcg
    19861 ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa tcgccagtca
    19921 ttgattgatg cggggatgtt ggtcactaac gatcagggac gcagttacga tcgtttccgc
    19981 gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt tggcgggcgc
    20041 gtgctgggca acgatacccc caaatacctg aactcgccgg aaacagacat tttccataaa
    20101 ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc caatcgtctg
    20161 cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat taattacgcc
    20221 gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt ccgcgcgacc
    20281 aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc atggcgagcg
    20341 ctggaaacgg cgctgcctta catgacagac ggccgtcagc tacgctttat gtttttgccg
    20401 gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt tgaagcgcgg
    20461 atggagcagg cgatgccgct ttccgcattt ctgtttaaca gcctgatgcc gcaagttgat
    20521 ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccatt gatatcgcaa
    20581 gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa attaggcata
    20641 cttgatgaca gccagcttga acgattaatg ccaaaagcgg cagagagcgg cgtttctcgc
    20701 cctgttccgc agctaaaacg cacgaccatg cgtatactta tagggttgct ggtgcaaaat
    20761 ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa gctccctgga
    20821 cttggcttat tcagagaact ggtcaacact tgtctctccc agccaggtct gaccaccggg
    20881 caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa actgtcgatg
    20941 tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc actcaaccat
    21001 atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga gcgcacgcat
    21061 ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct ggcgaaaaag
    21121 tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc gcactgagag
    21181 gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac aaccgttgga
    21241 tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc tcatgaaata
    21301 agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt cttgtcaccc
    21361 gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg ccggaagata
    21421 tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg ggcattcagg
    21481 tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc gcggacgaag
    21541 atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc gggcgcacga
    21601 ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg acccgcgaag
    21661 gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa tgctccgttg
    21721 ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt gaagcagaag
    21781 aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa gaagatctgg
    21841 cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac gatgacgaag
    21901 atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc atcgacccgg
    21961 aactggctcg cgaaaaattt gcggaactac gcgctcagta cgttgtaacg cgtgacacca
    22021 tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa ctgtctgaag
    22081 tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac agcatgcgcg
    22141 tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc gttgagcagt
    22201 gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc agcgatacct
    22261 ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac gatgtctctg
    22321 aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc ggcctgacca
    22381 tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa gcccgccgtg
    22441 cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc aagaaataca
    22501 ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg caacatcggt ctgatgaaag
    22561 cggttgataa attcgaatac cgccgtggtt acaagttctc cacctacgca acctggtgga
    22621 tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg caccatccgt attccggtgc
    22681 atatgattga gactatcaac aaactcaacc gtatttctcg ccagatgctg caagagatgg
    22741 gccgtgaacc gacgccggaa gaactggctg aacgtatgct gatgccggaa gacaagatcc
    22801 gcaaagtgct gaagatcgcc aaagagccaa tctccatgga aacgccgatc ggtgatgatg
    22861 aagattcgca tctgggggat ttcatcgagg ataccaccct cgagctgccg ctggattctg
    22921 cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg accgcgcgtg
    22981 aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa caccgaccac acgctggaag
    23041 aagtgggtaa acagttcgac gttacccgcg aacgtatccg tcagatcgaa gcgaaggcgc
    23101 tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg tagcttcctg gacgattaat
    23161 cggtaggccg gatcaggcgt tacgccgcac ccggcactag tccctctgca caaac
//
LOCUS       DAJTYZ010000063        22995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000063 DAJTYZ010000000
VERSION     DAJTYZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.063"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            230..1663
                     /gene="cscA"
                     /locus_tag="OO596_002297"
     CDS             230..1663
                     /gene="cscA"
                     /locus_tag="OO596_002297"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6718141.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1671..2729)
                     /locus_tag="OO596_002298"
     CDS             complement(1671..2729)
                     /locus_tag="OO596_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6718142.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2897..3115
                     /locus_tag="OO596_002299"
                     /pseudo
     CDS             2897..3115
                     /locus_tag="OO596_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3133..4461
                     /gene="dsdA"
                     /locus_tag="OO596_002300"
     CDS             3133..4461
                     /gene="dsdA"
                     /locus_tag="OO596_002300"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6718143.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="OO596_002301"
     CDS             complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="OO596_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6718144.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="OO596_002302"
     CDS             complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="OO596_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6718145.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7686..8300
                     /gene="evgA"
                     /locus_tag="OO596_002303"
     CDS             7686..8300
                     /gene="evgA"
                     /locus_tag="OO596_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6718146.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8305..11898
                     /gene="evgS"
                     /locus_tag="OO596_002304"
     CDS             8305..11898
                     /gene="evgS"
                     /locus_tag="OO596_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6718147.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11954..13099)
                     /gene="yfdE"
                     /locus_tag="OO596_002305"
     CDS             complement(11954..13099)
                     /gene="yfdE"
                     /locus_tag="OO596_002305"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6718148.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="OO596_002306"
     CDS             complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="OO596_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6718149.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="OO596_002307"
     CDS             complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="OO596_002307"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6718150.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15935..17185)
                     /gene="frc"
                     /locus_tag="OO596_002308"
     CDS             complement(15935..17185)
                     /gene="frc"
                     /locus_tag="OO596_002308"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6718151.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17698..18321)
                     /locus_tag="OO596_002309"
     CDS             complement(17698..18321)
                     /locus_tag="OO596_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6718152.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18618..18893
                     /gene="ypdI"
                     /locus_tag="OO596_002310"
     CDS             18618..18893
                     /gene="ypdI"
                     /locus_tag="OO596_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCR6718153.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18970..19212)
                     /locus_tag="OO596_002311"
     CDS             complement(18970..19212)
                     /locus_tag="OO596_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6718154.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19565..20485
                     /gene="lpxP"
                     /locus_tag="OO596_002312"
     CDS             19565..20485
                     /gene="lpxP"
                     /locus_tag="OO596_002312"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6718155.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO596_002313"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO596_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6718156.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO596_002314"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO596_002314"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6718157.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22592..>22995
                     /locus_tag="OO596_002315"
     CDS             22592..>22995
                     /locus_tag="OO596_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6718158.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKA"
BASE COUNT         6455 a         5382 c         4999 g         6159 t
ORIGIN      
        1 actttggctg acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct
       61 ttctcgcaaa atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag
      121 ttaacgttaa catttgtgat attcatcgca tttatgaaag taagggacta tatttttata
      181 aaagttaacg ttaacaattc accaaatttg cttaaccagg atgattaaaa tgacgcaatc
      241 tcgattgcat gcggcgcaaa acgcactagc aaaacttcat gagcaccggg gtaacacttt
      301 ctatccccat tttcacctcg cgccacctgc cggatggatg aacgatccaa acggcctgat
      361 ctggtttaac gatcgttatc acgcgtttta tcaacatcac ccgatgagcg aacactgggg
      421 accaatgcac tggggacatg ccaccagcga cgatatgatc cactggcagc atgagcctat
      481 tgcgctagcg ccaggagacg ataatgacaa agacgggtgt ttttcaggta gtgctgtcga
      541 tgacaatggt gtcctctcac ttatctacac cggacacgtc tggctcgatt gtgcaggtaa
      601 tgacgatgca attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga
      661 gaaacagggt gtgatcctca ctccaccaga aggaatcatg cacttccgcg atcctaaagt
      721 gtggcgtgaa gccgacacat ggtggatggt agtcggggcg aaagatccag gcaacacggg
      781 gcagatcctg ctttatcgcg gcagttcatt gcgtgaatgg accttcgatc gcgtactggc
      841 ccacgctgat gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga
      901 tcagcattat ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttaccgaaa
      961 tcgctttcaa agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc
     1021 cgggcatttt actgaacttg ataacgggca tgacttttat gcaccacaaa gctttttagc
     1081 gaaggatggt cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc
     1141 aaaacgtgaa ggatgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg
     1201 caaacttcta caacgcccgg ttcacgaagc tgagtcgtta cgccagcagc atcaatctgt
     1261 ctctccccgc acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat
     1321 tcagttgcag tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac
     1381 tggaatgcgg ctgtatattg ataaccaatc tgagcgactt gttttgtggc ggtattaccc
     1441 acacgagaat ttagacggct accgtagtat tcccctcccg cagcgtgaca cgctcgccct
     1501 aaggatattt atcgatacat catccgtgga agtatttatt aacgacgggg aagcggtgat
     1561 gagtagtcga atctatccgc agccagaaga acgggaactg tcgctttatg cctcccacgg
     1621 agtggctgtg ctgcaacatg gagcactctg gctactgggt taacataata tcaggtggaa
     1681 caacggatca acagcggaca agggatccgc gtcactcttc ccccttcacg accttcaata
     1741 atatgcaatg cagcttcccg cccgataatg tcatgtggaa gctgaattgt ggtcagcggc
     1801 ggtaaaaaca gatgcccgac gccaaccaga ttatcaaagc ccattacggc gacatcctgc
     1861 gggatacgta cccccttcgc caggagaacc tgataagcga caaaggctgc acgatcgtta
     1921 ccacatatca gaacatcaaa atctggtttg cccgatttga agtgggcatt gagtaaactt
     1981 gcgagatcgg tgtagtgatc atcacctgtt gccatgtgaa attgtttcac ctcagccaga
     2041 tctcgtccag catcacgcca ggcctgctca aatccctgcc gacgataccc tgttgccaac
     2101 gcactttccg gtagccagaa gcataacggt tgacgatagc ccgccgcgag caaatgctgt
     2161 gttgattcat attgtgcagt gtaatcatca gggatataac tgggtaacgc tgggtcatcc
     2221 gccacacagt tcgccaatac aatattttca ccatacagag actcaggcag cgtgatatgt
     2281 cgcagcccca ttgtagtata gataatgcca tccggacggt gggcaagcag ctgacgtgcc
     2341 gcgcgggcag cgtcatcttc agaaaaaata ttgattaaaa aactattcca gccgaactcg
     2401 ctggcggttt gctcaatggc aagcagaata tcaacagaga aaggagtggt agccgtgtcc
     2461 tgcgccagca cggcgagagt cgacggctta cgtccttgag cgcgcatctt acgggcggaa
     2521 agatcaggaa cataattcag ggtctggatt gcctgcaata cgcggtcacg cgttgcagga
     2581 cgcacagatt ctgcattatg catcacccgg gagactgtca tcatcgacac tcccgccagg
     2641 cgtgcgacat cctttaatga agccataccc aagccgtttg ccgtaaaacg ggcactgtag
     2701 cagaaacaga cgtcactggc gagatccaac gccctatcac ctgacacagc aatacaataa
     2761 aaaataacaa taattcccgg acaattgtcc ccaattccgc ctctgttctc gccaacgagt
     2821 aaccatatag gcaagtaagt aaaacatgcc aactggcatc gccaacctgg agaagaaaat
     2881 ataaatatgg atcattgtgc tgccgctgta tcccgacatc agcccggaaa ttattgcgat
     2941 tgctatcggt tcaggtgcaa ttggctgcac gatcgttacg gactcgcttt tctggctagt
     3001 gaagcaatat tgcggcgcta cgctcaatga aacatttaaa tactatacga cagcgacatt
     3061 tatcgcttca gtcgtcgctc tggcgggcac attcctgctg tcatttatca tctaagcgca
     3121 aagagacgta ctatggaaaa cgctaaaatg aactcgctca tcgcccagta tccgttggta
     3181 aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac ctcattggct
     3241 gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca tgcgcgctta
     3301 tcccgttttg caccctatct ggcaaaagca tttgctgaaa ctgctgccac tggggggatt
     3361 attgaatcag aactggttgc cattccagct atgcaaaaac ggctggaaaa agaatatcag
     3421 caaccgatca gcgggcaact gttactgaaa aaagatagcc atttgcccat ttccggctcc
     3481 ataaaagcac gcggcgggat ttatgaagtc ctggcacacg cagaaaaact ggctctggaa
     3541 gcggggttgc tgacgcttga tgatgactac agcaaactgc tttctccgga gtttaaacag
     3601 ttctttagcc aatacagcat tgctgtgggc tcaaccggaa atctggggtt atcaatcggc
     3661 attatgagcg cccgcattgg ctttaaggtg acagttcata tgtctgctga tgcccgggca
     3721 tggaaaaaag cgaaactgcg cagccatggc gttacggtcg tggaatatga gcaagattat
     3781 ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg acccgaactg tttctttatt
     3841 gatgacgaaa attcccgcac gttgttcctt gggtattccg tcgctggcca gcgtcttaaa
     3901 gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata accctctgtt tgtctatctg
     3961 ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg ggcttaaact ggcgtttggc
     4021 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
     4081 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
     4141 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
     4201 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
     4261 caggaagaag gtattcgtct tgaaccttcg gcactggcgg gtatggccgg acctcagcgc
     4321 gtatgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca actgcgtaat
     4381 accactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
     4441 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
     4501 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
     4561 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
     4621 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
     4681 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accataatat
     4741 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
     4801 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
     4861 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
     4921 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
     4981 ccctgaaaaa actgcggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     5041 gaacgccagt aatagcaaac cgcatacatc aaaaaactaa atgtcactaa caaccgcatg
     5101 tctattttgt tgccataacg tcctatcaaa ggtgaaatta atagtggcat gatgccgatg
     5161 ggcgcataag caagtccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     5221 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc atgtgatact cacaatacca
     5281 atggtgaagt tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     5341 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     5401 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     5461 ccgagcacta acagggtcag tcctggtaga ttcattttga ccggtgaagt ctcagtttct
     5521 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     5581 aaaaatatcc aaccccagct aaagttatca caaatataac cgcccaatat cggcccacat
     5641 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggc
     5701 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaacccc
     5761 tgaacgactc taaaaaatat cagcacatca agattggtcg ataggctaca cattaatgaa
     5821 gacagcgaaa aaaaggttac tgaaagtaaa aataatctta attcgcctat tctttgtgcc
     5881 aacctgccag taacagggat cgcaatggca tttgctacac caaacgaggt gataacccag
     5941 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     6001 atagtggaat ccaacatttg cataaatgtc gctaatgaca atgcaatagt gacgcaccat
     6061 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     6121 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     6181 tactggtata agccggcatg gaggtcacgg ttgaagccag ctcaggcatc tcagcaatgt
     6241 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     6301 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     6361 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     6421 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     6481 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     6541 gacgtgccgg tacgacagcc attaacgatt gtccgggact cactgtttcg ccgacctgaa
     6601 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcgcca
     6661 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     6721 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     6781 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     6841 tcgctaaagg cacacgacgg ttataatctt ctaaggattg ttgatactga atacgtgctg
     6901 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     6961 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     7021 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     7081 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     7141 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     7201 aaactatcac caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa
     7261 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
     7321 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag
     7381 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
     7441 caattcttac gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat
     7501 catcggtata atccctgatt ttattgttga catttcattt atgccgacta tttatatggt
     7561 atacttgtcg aattatctta aaggaagctc agattttctt atttttattg agaaaatgag
     7621 atgacgcctt atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat
     7681 aatctatgaa cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt
     7741 tattgatcaa aaacgatatt gaaatcttag cggagttaac tgaaggcgga agcgccgttc
     7801 agcgggtgga aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta
     7861 acggtatcct ggtgttagaa acgctgagga agcgccaata tagcggaatt attattatcg
     7921 tctccgctaa aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt
     7981 tcgtgagtaa aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg
     8041 gctactgcta tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc
     8101 aaaaactcga ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca
     8161 aggataataa tgacattgcg gaaaaaatgt tcatcagcaa caaaactgtc agcacttata
     8221 aaagtcgcct gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcac
     8281 aacgtaacaa aatcggctaa ccacatgaag tttttaccct atatttttct tctctgttgt
     8341 ggtctttggt cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt
     8401 agtaacaacc gtgtcatact tgatccagta cgtctgagca acaaggaatt acgttggtta
     8461 gcgagcaaaa aaaatcttgt gattgcagta cataagtccc aaacggctac gttgttgcat
     8521 accgattcgc agcaacgagt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga
     8581 acgttaaata tcaaattaac actccgggaa tacgccgatc atcaaaaagc aatggacgcg
     8641 ctggaagaag gtgaagtcga tatagtgtta tcacatttag tagcttcgcc gcctcttaat
     8701 gatgacattg ctgcaaccaa accactgata attacctttc cggcgctggt aaccaccctt
     8761 cacgattcaa tgcgaccgct tacctcatca aaaccagtaa atattgctcg agtagcaaat
     8821 tatccccctg acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca
     8881 aatttatatc aggcattagc atccgtctca gccggacaga atgattactt tattggtagt
     8941 aacatcatta ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa
     9001 tattataact caccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcgttctt
     9061 aatgaagtac tcaatcgatt tgttgatgct ttgacaaatg aagttcgcta tgaagtatca
     9121 caaaattggc ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa
     9181 catgaaaaac aatggattaa gcagcatccc gatttaaagg tgctggaaaa tccttactcc
     9241 cctccctatt ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggatattctt
     9301 aatattatta ctttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc
     9361 catgctggaa cacagctgaa tcccggcgga tgggatatat tacccggtgc tatttatagt
     9421 gaagatagag aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt
     9481 gtcatgcaaa aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt
     9541 ccatattatt atgagcttca ttcgcaatta aaagagatgt atccggaggt agagtggata
     9601 aaagtcgata acgccagcgc tgcatttcac aaggtcaagg aaggcgaact tgatgctctg
     9661 gtcgcgacac agttaaattc acgttacatg atcgaccatt actatcctaa tgaactttat
     9721 cattttctta ttcccggcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa
     9781 ccggaactta aggatattat taataaagca ctgaatgcaa tccccccaag cgaagttctg
     9841 cgcctgaccg aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctctat
     9901 agcgagcaat tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta
     9961 tggggatttt acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa
    10021 aaccaaatat cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta
    10081 aactggcaag gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat
    10141 tactacaaaa atgcaatgtt accattagaa aacagtgact ccccctttaa agatgttttt
    10201 tctaatgcgc atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggtt
    10261 tttgaaattg ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat
    10321 ctgccagcca gcgatcatgc tgtttatatt tgtggttggc aagatattac agagacgcgt
    10381 gatttaattc atgcactcga agtagagaga aataaagcga tcaatgcaac tgtcgcaaaa
    10441 agtcagtttc tggcaacaat gagtcacgaa ataagaacac caataagctc cattatgggc
    10501 ttcctggaac ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca
    10561 cttgcctact ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac
    10621 aaaattgaat cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc
    10681 cagaacactt gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaatttgc
    10741 agcagtacat ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc
    10801 ttatcaaatt tactgagcaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg
    10861 acctccctgg ttcacattga tgacaaccac gctgttatca aaatgacgat tatggattct
    10921 ggaagtggat tatcgcagga agaacaacaa caactcttta aacgctacag ccaaacaagt
    10981 gcaggtcgcc agcaaacagg atctggctta ggcttaatga tctgcaaaga attaattaaa
    11041 aatatgcagg gcgatttgtc attagaaagt catccaggca taggaacaac atttacgatc
    11101 acaatcccgg tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacacccc
    11161 atcacactac ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta
    11221 ttactcaaac gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg
    11281 caagcgctac acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg
    11341 ccgaatatgg atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc
    11401 atctgggggc ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc
    11461 atgaacttat gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag
    11521 ttacaccagg ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa
    11581 aataatacgg cgaacgatct acaactgatg caggagattc tcatgacttt ccagcatgaa
    11641 acgcataaag atctacccgc tgcgtttcaa gcactagaag ctggcgataa cagaactttc
    11701 catcagtgta ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt
    11761 aatattagcc atcagttaga aataacacct gtttcagatg acagtaagcc tgaaattctt
    11821 cagttgctga actctgtaaa agaacacatt gcagagctgg accaggagat tgctgttttc
    11881 tgtcagaaaa atgactaaat agcggctccc acaatgttct aatgtgggag ctatttacca
    11941 gcacatctta catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg
    12001 ttgccgctcc cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg
    12061 gcatcattat tcctccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa
    12121 tggcctcagc cacacttaat aacggcgcga cgggcacacc aacttcatgt attttggcta
    12181 accaaacttc agctgcctgc gtttttaacg ttcgctcaat atattgttta agaatagcct
    12241 ggttttgtac gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct
    12301 ccagtgcctg gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct
    12361 tatcctgagt atcgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccaggcgtt
    12421 gtggtgactt ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg
    12481 catcaaacat cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg
    12541 cactcactat tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa
    12601 cacgcactgg cggagcatca gggtaccctg tttccatcat tatcccactc attgcctgaa
    12661 tgatggtatc gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg
    12721 aagcatatat gaggcgcggg ttgatttctt gtaacctttc ccatgaaaac cccagttttt
    12781 ccattgtacc tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
    12841 atatactttt atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa
    12901 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
    12961 catgacctgg tggctcaact ttaattaccc ttgcgcccat attacaaaga agttgagttc
    13021 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
    13081 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
    13141 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
    13201 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
    13261 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
    13321 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
    13381 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
    13441 aactcgaatt tatgtgctgc cagagtcaac cccgcagcga ataccgctac ccctgagtta
    13501 gccttcgcaa tcagattaaa ggtcgggtcc catgctgccg gaatttttac cccaaccagc
    13561 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
    13621 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
    13681 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
    13741 gatacggaat caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt
    13801 gaacccgcaa ttaatgcaca tacagcggct tctgcatggg tacgtttaaa aaatttgtag
    13861 caaccaaacc aagagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
    13921 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
    13981 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    14041 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    14101 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    14161 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    14221 gtaatatggc cactttcagt tcctgcagca gggtcgatga ccacattaat aatggtcggt
    14281 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    14341 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    14401 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    14461 ttattaaaaa taacgatcgt caccggcagg ttatatcgac aaatcgtttc aatttccatc
    14521 ccactgaaac caaaagcact atcaccttca atggcgacaa ccggagaacc agaagtcacg
    14581 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    14641 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    14701 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    14761 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    14821 tgctgcttgt ggatatttaa aatatcgcgc cataccagtg gagtcgtaaa tgtgttttgt
    14881 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    14941 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    15001 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    15061 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    15121 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    15181 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    15241 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    15301 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    15361 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    15421 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    15481 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    15541 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    15601 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    15661 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    15721 tcatgacgaa aaccaatata acgaatgcct tccgcctggg catggcgtgc catatccgtc
    15781 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    15841 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    15901 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    15961 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    16021 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    16081 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    16141 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    16201 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    16261 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgaa tcggtaatcc
    16321 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    16381 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    16441 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    16501 gcgggtattc ttccagataa cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    16561 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    16621 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    16681 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    16741 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    16801 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    16861 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    16921 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    16981 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    17041 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    17101 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    17161 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    17221 ttagcgttta tttaaggcat aacgccctct ataaataatc tgcaaaatat cttcgtagcg
    17281 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gccacttcca
    17341 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tatttattcg
    17401 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    17461 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    17521 taaatgattt gtgatgacac tggattattg cggtttactt tgaggaaaat aaaatgcgtg
    17581 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    17641 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    17701 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    17761 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    17821 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    17881 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    17941 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    18001 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    18061 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    18121 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    18181 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    18241 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    18301 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    18361 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    18421 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgct
    18481 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    18541 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    18601 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    18661 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    18721 ataaagagac tgaaaaaaca tcagcgatga caaccacaca ctctacaaaa cttcagccat
    18781 cacaggcgat tttgtttaag atgagagaag atgcgccacc attaaacctc acagaagaaa
    18841 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt
    18901 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    18961 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    19021 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    19081 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    19141 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    19201 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    19261 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    19321 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    19381 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    19441 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    19501 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    19561 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    19621 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    19681 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    19741 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    19801 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    19861 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ctaaacgcgc
    19921 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    19981 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    20041 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
    20101 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    20161 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    20221 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    20281 aacgatggta agaaaagcgg attacagcgg atatcgtttg tttatcaccc cagagatgga
    20341 aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga
    20401 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    20461 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    20521 cataataacc tcatggtttt tatggctttt ttcttaaggt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattaa cgccgtcttg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacggcg
    21301 gcgtttgtac tgttcggtaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atccatggct ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cgacgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagc
//
LOCUS       DAJTYZ010000064        22285 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000064 DAJTYZ010000000
VERSION     DAJTYZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.064"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="OO596_002316"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="OO596_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6718159.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="OO596_002317"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="OO596_002317"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6718160.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="OO596_002318"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="OO596_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6718161.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="OO596_002319"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="OO596_002319"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6718162.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="OO596_002320"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="OO596_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6718163.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="OO596_002321"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="OO596_002321"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR6718164.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8676)
                     /gene="eco"
                     /locus_tag="OO596_002322"
     CDS             complement(8188..8676)
                     /gene="eco"
                     /locus_tag="OO596_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6718165.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(8811..8975)
                     /locus_tag="OO596_002323"
     CDS             complement(8811..8975)
                     /locus_tag="OO596_002323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718166.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="OO596_002324"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="OO596_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6718167.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECCFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..9831
                     /gene="napD"
                     /locus_tag="OO596_002325"
     CDS             9568..9831
                     /gene="napD"
                     /locus_tag="OO596_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6718168.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9828..12314
                     /gene="napA"
                     /locus_tag="OO596_002326"
     CDS             9828..12314
                     /gene="napA"
                     /locus_tag="OO596_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6718169.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12321..13016
                     /gene="napG"
                     /locus_tag="OO596_002327"
     CDS             12321..13016
                     /gene="napG"
                     /locus_tag="OO596_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6718170.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13003..13866
                     /gene="napH"
                     /locus_tag="OO596_002328"
     CDS             13003..13866
                     /gene="napH"
                     /locus_tag="OO596_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6718171.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13863..14312
                     /gene="napB"
                     /locus_tag="OO596_002329"
     CDS             13863..14312
                     /gene="napB"
                     /locus_tag="OO596_002329"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6718172.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14322..14924
                     /gene="napC"
                     /locus_tag="OO596_002330"
     CDS             14322..14924
                     /gene="napC"
                     /locus_tag="OO596_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6718173.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14943..15560
                     /gene="ccmA"
                     /locus_tag="OO596_002331"
     CDS             14943..15560
                     /gene="ccmA"
                     /locus_tag="OO596_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6718174.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15557..16219
                     /gene="ccmB"
                     /locus_tag="OO596_002332"
     CDS             15557..16219
                     /gene="ccmB"
                     /locus_tag="OO596_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6718175.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16261..16998
                     /gene="ccmC"
                     /locus_tag="OO596_002333"
     CDS             16261..16998
                     /gene="ccmC"
                     /locus_tag="OO596_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6718176.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16995..17204
                     /gene="ccmD"
                     /locus_tag="OO596_002334"
     CDS             16995..17204
                     /gene="ccmD"
                     /locus_tag="OO596_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6718177.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17201..17680
                     /gene="ccmE"
                     /locus_tag="OO596_002335"
     CDS             17201..17680
                     /gene="ccmE"
                     /locus_tag="OO596_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6718178.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17677..19620
                     /gene="ccmF"
                     /locus_tag="OO596_002336"
     CDS             17677..19620
                     /gene="ccmF"
                     /locus_tag="OO596_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6718179.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19617..20174
                     /gene="dsbE"
                     /locus_tag="OO596_002337"
     CDS             19617..20174
                     /gene="dsbE"
                     /locus_tag="OO596_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6718180.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20171..21223
                     /gene="ccmH"
                     /locus_tag="OO596_002338"
     CDS             20171..21223
                     /gene="ccmH"
                     /locus_tag="OO596_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6718181.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21258..21905)
                     /gene="narP"
                     /locus_tag="OO596_002339"
     CDS             complement(21258..21905)
                     /gene="narP"
                     /locus_tag="OO596_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6718182.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         4956 a         5620 c         6350 g         5359 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc tgcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     9841 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     9901 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     9961 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
    10021 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
    10081 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
    10141 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
    10201 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa tcgatcggta
    10261 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgtttaaag
    10321 cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg tcggcagtag
    10381 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
    10441 aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg atcctctggt
    10501 cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg ctttctacct
    10561 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
    10621 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
    10681 tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
    10741 cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga
    10801 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaacc gctgaaatga
    10861 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
    10921 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggctaaca
    10981 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
    11041 tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
    11101 accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc tgcgagaaga
    11161 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
    11221 accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc aacaacatgc
    11281 aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
    11341 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga
    11401 ccgcaatgtg ggtagagaaa gagggtgctt acggtaacgc cgaacgccgt actcagttct
    11461 ggcgtcagca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag ttagtccagt
    11521 tctcccgccg cttcaaaact gaagaagtgt ggccggaaga gctgctagcg aagaaaccgg
    11581 aactgcgtgg caaaacgctg tacgaagtgc tttatgccac accggaagtg agcaaattcc
    11641 cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
    11701 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg
    11761 cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
    11821 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
    11881 agttctacgg taaaccggat ggtaaagcgg tgatcttcgc gctgccgttc gaaccggcgg
    11941 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
    12001 ggcacactgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
    12061 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
    12121 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
    12181 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactaa
    12241 cgctggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
    12301 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
    12361 ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
    12421 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
    12481 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
    12541 acaccttaaa actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg
    12601 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccgagtg
    12661 gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg gcggtgctgg
    12721 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
    12781 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
    12841 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggctgcggt aagtgcgaaa
    12901 aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
    12961 agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt
    13021 gacgccgggc gcgaggcgct ggtgaaaaaa ggctggtggc gcagtcaccg ttggctggtg
    13081 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt
    13141 gtgtggatct tacacggcaa ctacagctgt agcctgttat tcgacaccgt tccgctgacc
    13201 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
    13261 accggtgcgg tgataatcac tgtgctctac gccctggcag gaaagcgatt attttgcagc
    13321 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
    13381 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
    13441 ctgggatcag ccttaaccgg cacgctcatc tgggaatgga tcaaccccgt ttccctgatg
    13501 gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
    13561 tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg
    13621 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccagcgatcg gcagaaatgt
    13681 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
    13741 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
    13801 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
    13861 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
    13921 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
    13981 ccgggacgca ggaaggggcc attcggatgc caaaagagca ggatcggatg ccgctgaact
    14041 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
    14101 ccaaccgctg cttgcagtgc cacggtgtcg aaagctatcg caccactggc gcgccgcgta
    14161 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
    14221 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
    14281 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
    14341 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
    14401 gaacgctgct gttgatcggt tttgtcggcg gcatcgtctt ctggggcggc tttaacaccg
    14461 gaatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgtaacacgg
    14521 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
    14581 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
    14641 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
    14701 atcgtctgac aatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
    14761 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
    14821 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
    14881 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
    14941 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
    15001 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
    15061 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
    15121 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
    15181 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
    15241 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
    15301 acgatattcc ggtaaatcaa ctttctgccg ggcaacaacg ccgcgtcgct ttagcgcgtc
    15361 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
    15421 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
    15481 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
    15541 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
    15601 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
    15661 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
    15721 ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
    15781 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
    15841 gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt
    15901 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
    15961 gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa
    16021 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
    16081 tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
    16141 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
    16201 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
    16261 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
    16321 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
    16381 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
    16441 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
    16501 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
    16561 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
    16621 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg
    16681 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
    16741 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
    16801 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
    16861 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
    16921 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
    16981 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
    17041 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
    17101 tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
    17161 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
    17221 ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct
    17281 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
    17341 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtttg cgcgttggcg ggatggtgat
    17401 gctgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc
    17461 tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
    17521 gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct
    17581 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca
    17641 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg
    17701 ctgctgtgtc tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg
    17761 gcgcgcggag atgcgcgcat gatcgcgtct tcccgcttgt ttgcctggct gctgtttatg
    17821 tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc
    17881 acctatgttg ccagctactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
    17941 tggggcgcgc atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt
    18001 gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
    18061 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca
    18121 cgcacgctgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggacccg
    18181 gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt
    18241 gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc gcgttttact
    18301 cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct cggttccgca
    18361 tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt agaaaacgcc
    18421 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
    18481 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
    18541 ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcgtctgat
    18601 ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg tgattggagg ttcgctgctg
    18661 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
    18721 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtgttgctg
    18781 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    18841 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctact gctgggtgtc
    18901 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    18961 gccttcatct caacgctggt gctgtctctg ctgttgccgt ggctgtttga gagcaaagtt
    19021 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgcggtgct ggcaattgcg
    19081 gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct tcagttattg ggggatggtg
    19141 gctgctcacc tggggctggc agtgacaatt gttggcattg cttttagcca gaactatagc
    19201 gttgagcgtg atgtgcgcat gaagtccggc gatagcatcg atattcatga atatcgcttc
    19261 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    19321 ggcgtaacgc gagagggtaa accggaaaca gtgctgtatg cggaaaaacg ttattacaac
    19381 actgccgggt cgatgatgac cgaagcggcg attgacggtg gcatcacgcg tgacctgtac
    19441 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggctg tgcgtcttta ctacaaacca
    19501 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg
    19561 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga
    19621 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    19681 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    19741 gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
    19801 cggatgtgct gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga
    19861 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtagtcg
    19921 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    19981 cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc ggtgtctatg
    20041 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    20101 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    20161 aggccgcaca atgaggtttt tattgggcat gctgatgctg atgatctccg gctcagcgct
    20221 ggcgaccata gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    20281 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
    20341 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa
    20401 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    20461 aacgccgctg accgtgctgc tgtgggtgct gccagtagta gctattggca ttggcggttg
    20521 ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    20581 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    20641 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    20701 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagctga
    20761 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
    20821 aaatccggga gatatagacg gctggattat gttgggccgc gttggcatgg cgctgggtaa
    20881 cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga
    20941 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    21001 cggcggcgaa ctgctacgtc agctggtgag aacggaccat agcaatatcc gtgtgctaag
    21061 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    21121 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    21181 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg
    21241 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
    21301 acgcgtgagc ggacattgag tttacgcagc agatttcgaa tatgaacttt tactgtctgc
    21361 tcggaaatat tcaacacaga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    21421 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    21481 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg
    21541 gcacgaatcg cttccagcaa cacttccggg tcgctgtctt tcagcagata accgtctgcg
    21601 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
    21661 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    21721 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    21781 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    21841 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    21901 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    21961 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    22021 cttgccgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    22081 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    22141 aaaactgact ccgggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    22201 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    22261 gcaatatctg ttctggtggc tacag
//
LOCUS       DAJTYZ010000065        22090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000065 DAJTYZ010000000
VERSION     DAJTYZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22090
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.065"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..1463
                     /locus_tag="OO596_002340"
     CDS             75..1463
                     /locus_tag="OO596_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6718183.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="OO596_002341"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="OO596_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6718184.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /locus_tag="OO596_002342"
     CDS             complement(2010..2114)
                     /locus_tag="OO596_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718185.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="OO596_002343"
     CDS             2284..2730
                     /locus_tag="OO596_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6718186.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="OO596_002344"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="OO596_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR6718187.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="OO596_002345"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="OO596_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR6718188.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="OO596_002346"
     CDS             4841..5188
                     /locus_tag="OO596_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6718189.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="OO596_002347"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="OO596_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6718190.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..6672
                     /gene="dgcP"
                     /locus_tag="OO596_002348"
     CDS             5647..6672
                     /gene="dgcP"
                     /locus_tag="OO596_002348"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6718191.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6705..6803
                     /locus_tag="OO596_002349"
     CDS             6705..6803
                     /locus_tag="OO596_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6718192.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6806..6880
                     /gene="yoaJ"
                     /locus_tag="OO596_002350"
     CDS             6806..6880
                     /gene="yoaJ"
                     /locus_tag="OO596_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6718193.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6939..7187)
                     /locus_tag="OO596_002351"
     CDS             complement(6939..7187)
                     /locus_tag="OO596_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6718194.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7335..7517)
                     /locus_tag="OO596_002352"
     CDS             complement(7335..7517)
                     /locus_tag="OO596_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCR6718195.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="OO596_002353"
     CDS             complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="OO596_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCR6718196.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="OO596_002354"
     CDS             complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="OO596_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCR6718197.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="OO596_002355"
     CDS             complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="OO596_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCR6718198.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9844..10929
                     /gene="dmlA"
                     /locus_tag="OO596_002356"
     CDS             9844..10929
                     /gene="dmlA"
                     /locus_tag="OO596_002356"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCR6718199.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11180..12790
                     /gene="yeaV"
                     /locus_tag="OO596_002357"
     CDS             11180..12790
                     /gene="yeaV"
                     /locus_tag="OO596_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCR6718200.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12822..13946
                     /gene="yeaW"
                     /locus_tag="OO596_002358"
     CDS             12822..13946
                     /gene="yeaW"
                     /locus_tag="OO596_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCR6718201.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14002..14967
                     /gene="yeaX"
                     /locus_tag="OO596_002359"
     CDS             14002..14967
                     /gene="yeaX"
                     /locus_tag="OO596_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6718202.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="OO596_002360"
     CDS             complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="OO596_002360"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6718203.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="OO596_002361"
     CDS             complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="OO596_002361"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6718204.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="OO596_002362"
     CDS             complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="OO596_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6718205.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="OO596_002363"
     CDS             complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="OO596_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6718206.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19482..21392)
                     /locus_tag="OO596_002364"
     CDS             complement(19482..21392)
                     /locus_tag="OO596_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6718207.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21524..21868
                     /locus_tag="OO596_002365"
     CDS             21524..21868
                     /locus_tag="OO596_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6718208.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5402 a         5664 c         5379 g         5645 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc ataaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacact atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     6661 ccacattgct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     6721 tttttccggt tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg
     6781 gctatgctgc cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc
     6841 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc
     6901 aaaggctgcc tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag
     6961 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     7021 tcgactttac caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc
     7081 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     7141 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     7201 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     7261 aagatttgtg acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca
     7321 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc
     7381 acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc
     7441 ggcgtctctg tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta
     7501 tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct
     7561 tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg
     7621 gcttcaatgt tgtgccactt ttctggaggg aacacctcaa actgccccgc ttcgataagg
     7681 atcacctgat caggctctgc actgtgttca tcagcgtagc cgagatattt gaccgcccca
     7741 tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg
     7801 aatattccgg caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa
     7861 ttctgtggga tttgcagcat ccttttccct ccttcggtga atgcgctgaa aacggtttat
     7921 tccagccgtt tcagggtacg cctgataatt tgcattttaa ataccattta ttggttactt
     7981 tttagcacca tatcagagaa aaatcaggga ggattacaag tgggaagccc gcgacaacgc
     8041 gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa
     8101 accaatcagt gagttgccaa ctttagccag tttctttttg gtacgtatgt actgcgtgac
     8161 aaaagcacca gaaataatca ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt
     8221 cgccagaata aagaatgaaa ttcccgtatg tggggcatta acatcgataa actgtacgaa
     8281 aaacgacaca tagaacaaaa tggctttcgg attagtcagg ctcaaaatta acgcgcgttt
     8341 aaaaatggcc ccgtattggg gctcatcgga tttggcctcg ttatttttac ccttcagggt
     8401 cgcgtagaga attttactcc ccagatagag caaataaaac gcaccaagat aacgtacaat
     8461 gttgaataat atcggggtgg tcttaattaa tgtcgccact ccagcccatg ccagaaacat
     8521 caatactgca tcgccaataa atacaccgca ggccgcaaga taaccgcctt tcataccgct
     8581 actgacgcta tttttgagta caaacagggt atttggccct ggcaccaaca caataaaaat
     8641 ggccccaacc agataggtcc agtaattcag aaccccgtat tcagcgaaca cattaacctc
     8701 tttaattatc tttcgatcat gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc
     8761 ttagaaagga gaaaatcagg ttttaacccg atatcaaccc gataattgaa taattaatca
     8821 ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta aaaattctat
     8881 cgttatccgc actttcgctg acgtcgccag ccttgaaaca taaacggccc agacgttcgc
     8941 tggctggtaa tattccggta gcacttgcac taaatgacca ctggcaatgt tttcgctaac
     9001 atcccaccag gagcgcagag caatcccctg cccatcgaga caccactgat ggacaatttc
     9061 tccatgatta gacgacagtg ggccggtgac tttaatcgca tggtggcctt ctttattgcg
     9121 taattgccag acaccaaaag gatgatcgcg ctctttaatg accaggcacg gcaatgcgga
     9181 aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg acgcgcagag
     9241 aatgcggtaa ttagtcgcca gtttgcgggc gattaaatta ggggcgatgt catcgccaat
     9301 gcgaatatcg agatcaacac cttcattgac caaatcgacc aaccggtctt ccacatcaaa
     9361 acgtaattcc agttgcggat acgccttagc cagcgctgat aacgccggag ccaccacctg
     9421 tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc tgcggcacct gacgcacgtc
     9481 agaaagttcg tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc attcataaat
     9541 ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt
     9601 tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc
     9661 ttccgccacg gcggcaaaac cagcccggcg agccaccagc ataaagacgc gcaaatcgtt
     9721 cagcagcggt aaattattca tgattcgtgt tttatgtttc accagttacg gggattaatt
     9781 ccttttcagt caattatagg atggtgatgt tgtcaatttt gatggtcagg aagtgagaac
     9841 ccaatgatga aaacgatgcg tattgctgcg atcccgggag atgggattgg caaagaagtc
     9901 cttcctgaag ggattcgcgt attacaggct gccgctgagc gctggggctt cgacttgagt
     9961 tttgagcaaa tggagtgggc gagctgcgag tattacagcc atcacggtaa aatgatgccg
    10021 gacgactggc atgagcaact tagccgtttc gacgccatct attttggtgc cgtcggctgg
    10081 ccggataccg ttccggacca tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa
    10141 ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg
    10201 gcgggaaaac agcctggcga catcgatttt tacgtggtca gggaaaacac cgaaggcgaa
    10261 tattcctcgc tcggcggtag agtgaatgaa ggtacagagc atgaagtcgt cattcaggaa
    10321 tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa
    10381 agccgcccac gtaagacgct aacttctgcc actaaatcaa acggtttagc catcagcatg
    10441 ccgtactggg atgagcgagt ggaagcaatg gccgagaatt acccggagat ccgctgggac
    10501 aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg
    10561 gtggtggcgt ccaatttgtt tggcgatatc ctttccgatc ttggcccggc ctgcaccggc
    10621 accattggca ttgccccatc cgccaacctg aatccggaac gcactttccc gtcgctcttc
    10681 gagcctgtcc acggttccgc gccggatatc tacgggaaaa atattgctaa ccctatcgcc
    10741 acaatttggg ccggtgcgat gatgctcgat tttctcggca atggcgatga gcgtttccag
    10801 caagcgcata acggtattct ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca
    10861 ccggatatga aaggcagtgc caccacgcca caggttgccg acgcgatttg caaaattatt
    10921 ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact gacgcggttt ttttattcgt
    10981 tctttgcagt taataacctg cgtcatttca ccttttattg tttccgtttc gtgttttatg
    11041 gctttccgta ttcttaattg tttaatttat gtaacatgca aataatcaat ttgttaaaca
    11101 caacatacaa ctcattaccc tgcaaataat tacaactggc aaaagccaga gacgggcttt
    11161 tatgcccagg agatgactca tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag
    11221 catcagcctg gtggccattc ttttcatcgc agctgcatta agccttttcc cacaacaatc
    11281 ggccgacgcg gccaacacaa tatacacttt tgttacgcgt acgttaggtt ccgccgtaca
    11341 ggtattggtt ttgctggcaa tgggactggt gatttattta gccaccagta aatacggcaa
    11401 tattcgtctt ggcgaaggaa aaccggaata cagcacgctc tcctggctgt ttatgtttat
    11461 ttgtgccggt ttaggttctt ctacgcttta ttggggggtt gctgaatggg cctattatta
    11521 tcaaacgcct ggattaaata tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc
    11581 ttactccttc ttccactggg gcatcagcac ctgggcaact tatacgctgg cctcattaat
    11641 catggcttat cactttcatg tgcgtaaaaa caaaggtctg agtctttccg gcattattgc
    11701 cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa ctggttgatt tgatgtttct
    11761 gatcgccact gtcggcgcac tgactatttc ccttgttgtc accgcagcaa cctttactcg
    11821 tggactttcc gcgctgaccg gtttacccga taacttcacc gtgcaggcat ttgtgatcct
    11881 gctttccggc ggcatttttt gcctaagctc gtggattggt atcaacaacg gtttgcaacg
    11941 tctgagcaaa atggttggct ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg
    12001 tccaaccgaa tttattacca acagcatcat caatgccatc ggcctgacca cgcaaaactt
    12061 cctgcaaatg agcttattca ccgatccgct tggcgatggt tcatttaccc gcaactggac
    12121 cgttttctac tggctgtggt ggatctcata cccccctggc gtagcaatgt ttgtcacccg
    12181 cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt
    12241 cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc attcatcagt ttatcaatgg
    12301 cgtaatcaac gtcccacagg tgctggaaac actgggcggc gaaacagctg tacagcaagt
    12361 tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca tacctgggcg tgatgattat
    12421 tttccttgcc tcgcatatgg atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa
    12481 tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac
    12541 tctgatcccg ctttccatct tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt
    12601 cgtgctcaca gcccttccct tcctcgtcat tttactggtg aaagtcggcg gatttattcg
    12661 ctggctgaaa caggattatg ccgacattcc ggctcatcaa gttgaacatt atctcccgca
    12721 gacaccggtt gaagccctgg aaaaaacacc agtgctccct gcgggaaccg tattcaaagg
    12781 cgacaactga gcgccatcat cctaacgata aaggtatccc tatgagcaat ctgagccctg
    12841 actttgtact acccgaaaat ttttgcgcta acccgcaaga ggcgtggacc attcctgccc
    12901 gtttttatac cgatcagaac gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct
    12961 ggatttgcgt cgctcacagc agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga
    13021 tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata
    13081 acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga aggaaaagca aaaaatgtga
    13141 ttacctgccc atatcacgca tgggcattca aactcgatgg caatctggcc catgcacgta
    13201 actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca actggttccg gtgcgtctgg
    13261 aggaatatgc cggattcgtc ttcatcaaca tggaccccaa cgccaccagc gtagaagatc
    13321 agttacccgg tctgggcgcg aaagtgctgg aagcctgccc ggaagtccac gatctgaaac
    13381 tggcggcccg ctttaccacc cgcacgcctg ccaactggaa gaacattgtc gataactatc
    13441 tcgagtgcta tcactgtggt ccggcgcatc caggtttctc cgactccgta caggttgatc
    13501 gctactggca caccatgcac ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg
    13561 aacagtcgtt taaatttgaa gagggtacgg atgcggcatt ccacggtttc tggctgtggc
    13621 cgtgcacgat gctgaacgtc accccgatca aagggatgat gacggtcatt tatgaattcc
    13681 cggtggattc tgaaactacc ctgcaaaact acgatattta cttcaccaat gaagagttaa
    13741 ccgacgagca aaaatcgctg attgagtggt atcgcgatgt gttccgtccg gaagatttac
    13801 gtctggttga aagcgtacag aaagggctga aatcgcgtgg ctatcgtggt caggggcgca
    13861 tcatggccga cagtagcggt agtggcattt ccgaacatgg tatcgcccat ttccataatc
    13921 tgctggcgca ggtgtttaag gactaatgac atcggcggcg gtattttccg ccgctgggct
    13981 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag
    14041 gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
    14101 ctgcctgcgt tcaccggagg aagtcacatc attgtgcaga tgagcgatgg tgataaccag
    14161 tacagcaatg cgtattcact actgagttcg ccgcatgaca cctcttgtta tcagattgcc
    14221 gttcggctgg aggaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
    14281 ggcgatcggt taacgatttc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
    14341 aagcatctgt ttatcgcggg cggtattggt atcacccctt tcctgtcgca catggcagag
    14401 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
    14461 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattcg
    14521 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
    14581 gtttatacct gtggccccga ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg
    14641 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
    14701 gcctttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
    14761 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
    14821 gtatgcggaa cctgcgaaac agcaatactg gaaggtgaag ctgaccatcg ggatcagtat
    14881 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
    14941 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
    15001 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
    15061 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
    15121 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
    15181 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
    15241 taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc cggcaatgtc
    15301 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
    15361 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
    15421 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc
    15481 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaattgcca ggcattggtg
    15541 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
    15601 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
    15661 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
    15721 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
    15781 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
    15841 gtgtcaatca ggggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
    15901 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
    15961 cagtcggtga tcccgagagg atcaattagc gccagatgct caccatcgaa aagttgaatc
    16021 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc
    16081 ggaaagacac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
    16141 taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
    16201 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
    16261 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
    16321 ttcggtactt tgtagcccgt gagctgacgg cagcaaaagg tcaccagtga ctcttcggta
    16381 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
    16441 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
    16501 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
    16561 aggaaccctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
    16621 atctcatcgg tagcatcggg acgctgccag taacccagca tcacctgcgg tcctttgaca
    16681 caaagctcac ccggttgacc cggtggtact tcattatcgt cgtcatccac cagtttggct
    16741 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atatgggtta
    16801 acgctgacca gcggcgcaca ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc
    16861 agtttcaccc aacgctctgc caccacttgc tgcactggca tcccaccgcc tgcggaaaga
    16921 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
    16981 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
    17041 atatcgcgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
    17101 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
    17161 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
    17221 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
    17281 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
    17341 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
    17401 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
    17461 attacgtgct gaacggcggt tttatcaacc actttttcca gtgtgtgagc aaagttagac
    17521 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
    17581 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa cagcgccacc
    17641 ggatattgca ataaattagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
    17701 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt
    17761 acctccccca tattcacaaa cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac
    17821 atatctatca gagattgata acggtcaggg ttgatctccg tcggaacatc cgcgggataa
    17881 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
    17941 accccaagtg ataaacatgt tgttaacata acattaactc atcttaccag cttgataatt
    18001 agacaacgaa aaggttgcga agcgcgtcac tatttatttt tatagttgcc gtatgaacgc
    18061 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caacttgtta ctcggttaca
    18121 attgtttcaa ctcttgcagg gccaggatta taccaacccc atccgccgta accatagggc
    18181 cagccgcgtc caccataaaa ccatggatca attggctgcg gcggcataat cacctgttga
    18241 gtgatatgcc agcgtttgta acctgttacc tgcataacca taaatttata tggtgcatta
    18301 tttactttgc catccactgt gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga
    18361 aaatcgaccg gatcaagaaa accgttcaca tcggcataaa tgcgcccgcg tgaaggttct
    18421 cccaaggttg gtcttgctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
    18481 ccttgctggt tttgtaccac aaccactttg ccgccaaagc gcgcctcctg accaacatat
    18541 aaatctggcg cattcatcac gcgtaccaga tcttgctgtg gtgttggact gctgccttta
    18601 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
    18661 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
    18721 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaacc
    18781 ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca cgcaatcggc
    18841 agcatatctt ccgccgctgg cagtaaaact tctccgtcgc gcaaaaccag cccgctctct
    18901 ttacggagat ccggccaggc ttgccagccc gtccctaccg ttacccattc gccggaaagc
    18961 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc
    19021 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
    19081 atggctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt
    19141 agcgtagaga caccaatcat cggtaactcc gcgccaagcg ccagcccttg cgcaatacca
    19201 ataccaatgc gcacgccagt aaagctaccg gggccgcgcc cgtaagccag agcgtttatg
    19261 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
    19321 gtatgttcac gagggcaaag ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg
    19381 acagagcaga cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
    19441 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
    19501 gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg
    19561 agaaacgtcg cgccgtaagg acgcatcacc agccgattgt cacaaattac cagcacgccg
    19621 cgatcgtcgg cgtcgcgaat cagtcgccct actccctgct tgagagtaat gaccgcatcc
    19681 ggcagctgca cttcatcgaa cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct
    19741 tttaacagta gatcatccgg cgaggtaaac ggcaatttgt cgataattac caatgacaat
    19801 gtatcgccac gcacgtccac cccttcccag aaactactgg ttgccacaag aagcgcgtta
    19861 ccggcgctga caaattgctg caacagttgc cctttgctgg tttccccctg caacaaaacg
    19921 ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc atgcgaggta
    19981 caaagcataa aacaacgacc gttgttagct tcgatgatcg gtcgcagcat tgccgccagt
    20041 tgccgtgcac aacccggttg attggtttgc ggcaggttgc gcggcacaca gagtaacgcc
    20101 tggcggctgt aatcaaacgg gcttggcaat agcaacgact ccgcctgttc tatcccaagc
    20161 cgcgaggtga aatgatgcag atcgtcgttc accgacagcg ttgctgaggt gaagatccag
    20221 ctaccgggct tttgcgccat taactctttg aatttatccg ccacgctgag cggcgtgaga
    20281 gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtagcccgg ctgattgatc
    20341 tcttttagcc gcttcagccg tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc
    20401 aaggcggaac gccccagtga cagtttcgcc acgtcataac aaagttccag ggtgtcatcg
    20461 agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag gttaccgcga
    20521 taacctggct caccgagttg cagacgaaaa tcctgtgcac tctgggcaag acgatcagcg
    20581 cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt gatgtctttt
    20641 gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct ggcaatgtcc
    20701 ggtagctggt gggcttcgtc gaagatcatg acgtccgctt cagggatcaa ctcgccaaat
    20761 ccactctctt taaccaccat atccgccaga aagagatgat ggtttaccac caccacatcg
    20821 gcgtccatcg cttttttacg tgctttgacc acaaagcaat ctttatacat cgggcagtcg
    20881 ctgccaagac agttgtcgtt ggtgctggtg accagcggcc acgcctgtga atcttccgcc
    20941 acgctgacgc aggtgctgat atcaccatcg actgtttgat tagaccagga gcgcagcagg
    21001 atcacatcgc ttaagatttg taccggcaga tcgccccccg ccagcgcctg ctgttcgaga
    21061 cgttcgaggc agaggtagtt tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc
    21121 aatgcctttg agactgttgg caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag
    21181 ccggtcgaga taatgacttt ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt
    21241 ttgcccgtac cggttcctgc ttccaccacc agcggctggc ctttttctat cgcctgggtg
    21301 acggctaccg ccatctgtcg ctgtggttct cgcggcttaa agcctggtat cgctttcgcc
    21361 agctgaccgt ctggtgcaaa atcgtccgtc acactacccc ctgttgattt gaacagggat
    21421 tatgtcagga tgagggcgct ttcgccagtt gaagtggtga cggcaacctc acattgtggc
    21481 agtctttgca gcacgaaaat ggaagtataa tgaggacaaa atgatgacta tcgttcgtat
    21541 cgatgctgaa gcccgctggt ctgatgtagt aatccacaac aacacgctct actacactgg
    21601 tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc gccaacacgc tggcacagat
    21661 tgacgccgtg ctggaaaaac agggcagcaa taaatcgagc attctggatg ccaccatttt
    21721 cctggccgat aaaaacgact tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc
    21781 gggtcatgcg ccagtgcgct gcacggtaca agcgggtttg atgaacccga agtataaagt
    21841 tgaaattaag attgtggctg cggtgtaagc tttatcgaag caaaataagt cagacgataa
    21901 tttatcgata atactggtcg gttttacata aatcgaccag agaataagat attactgttg
    21961 atgcttctgc taattcatta tttatattta taatttcaat tttatctata aaactaacta
    22021 tataaataaa cataaccatg ccgttgtgtt cgccctttga ttagcaactc tggttttctt
    22081 tcttagctga
//
LOCUS       DAJTYZ010000066        21858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000066 DAJTYZ010000000
VERSION     DAJTYZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.066"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            270..1388
                     /locus_tag="OO596_002366"
     CDS             270..1388
                     /locus_tag="OO596_002366"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6718209.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            1454..1702
                     /locus_tag="OO596_002367"
                     /pseudo
     CDS             1454..1702
                     /locus_tag="OO596_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            1767..2135
                     /locus_tag="OO596_002368"
     CDS             1767..2135
                     /locus_tag="OO596_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6718210.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2229..2882
                     /gene="nfsB"
                     /locus_tag="OO596_002369"
     CDS             2229..2882
                     /gene="nfsB"
                     /locus_tag="OO596_002369"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6718211.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            2990..4237
                     /gene="ybdG"
                     /locus_tag="OO596_002370"
     CDS             2990..4237
                     /gene="ybdG"
                     /locus_tag="OO596_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6718212.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="OO596_002371"
     CDS             complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="OO596_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6718213.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="OO596_002372"
     CDS             complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="OO596_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6718214.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="OO596_002373"
     CDS             complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="OO596_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR6718215.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="OO596_002374"
     CDS             complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="OO596_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6718216.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="OO596_002375"
     CDS             complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="OO596_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6718217.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12072..12755
                     /gene="cusR"
                     /locus_tag="OO596_002376"
     CDS             12072..12755
                     /gene="cusR"
                     /locus_tag="OO596_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6718218.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12745..14193
                     /gene="cusS"
                     /locus_tag="OO596_002377"
     CDS             12745..14193
                     /gene="cusS"
                     /locus_tag="OO596_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6718219.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            14930..16831
                     /locus_tag="OO596_002378"
     CDS             14930..16831
                     /locus_tag="OO596_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6718220.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            16859..17320
                     /locus_tag="OO596_002379"
     CDS             16859..17320
                     /locus_tag="OO596_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCR6718221.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            17340..21751
                     /locus_tag="OO596_002380"
                     /pseudo
     CDS             17340..21751
                     /locus_tag="OO596_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
BASE COUNT         5252 a         5864 c         5793 g         4949 t
ORIGIN      
        1 caaaatggct gtttatttat acagtaaatc ctgtttcgga cttctcactc ctcatttaat
       61 caactgaagc tgagcccgtt ttttctgcat gcctcaatcg tcaaaattct tcgccatcag
      121 ccagctctgc cataacttgc cacattgaaa aagtagccaa attgcttcag tcaccatcat
      181 caaaaaaaga aacattccag aacttccgca tctcgcccag ccgctccgac ttctatactg
      241 aatagaaaac gccaacataa gagaaaccta tgccattacc cgattttcat gtttctgaac
      301 cttttaccct cggtattgaa ctggaaatgc aggtggttaa tccgccgggc tatgacttaa
      361 gccaggactc ttcaatgctg attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa
      421 agcacgatat caccgaaagt atgctggagc tggcgacgga tgtttgccgt gatatcaacc
      481 aggctgccgg gcaattttca gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc
      541 atctggaaat ttgcggcggt ggcacacacc cgtttcagaa atggcagcgt caggaggtat
      601 gcgataacga gcgctatcaa cgaacgctgg aaaactttgg ctatctcatc cagcaggcga
      661 ccgtttttgg tcagcatgtc catgttggct gcgccagtgg cgatgacgcc atttatttgc
      721 tgcacggctt gtcacggttt gtgccgcact ttatcgccct ttccgccgcg tcgccatata
      781 tgcaaggaac ggatacgcgt tttgcctcct cacgaccgaa tattttttcc gcctttcccg
      841 ataatggccc gatgccgtgg gtcagtaact ggcaacaatt tgaagccctg tttcgctgtt
      901 tgagttacac cacgatgatc gacagcatta aagatctgca ctgggatatt cgccccagtc
      961 ctcattttgg cacggtggag gttcgggtga tggatacccc gttaaccctt agccacgcgg
     1021 taaatatggc gggattaatt caggctaccg cccactggtt actgacagaa cgcccgttca
     1081 aacatcagga gaaagattac ctgctgtata aattcaaccg tttccaggcc tgtcgctatg
     1141 ggctggaagg cgtcattacc gatccgcaca ctggcgatcg tcgaccgcta acggaagaca
     1201 ccttgcgatt gctggaaaaa atcgcccctt ccgcacataa aattggtgca tcgagcgcga
     1261 ttgaggccct gcatcgccag gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt
     1321 tcgtcgccga tggtggctcg ctgattgggc tggtgaaaaa gcattgtgag atctgggccg
     1381 atgactaagt agggaattgc cttacagaag aatatccctg acaatggtgt tttaacttac
     1441 atttaacaat gttatgaaac accctttaga aaccttgact accgcagcag gcattttgct
     1501 gatggctttc ctctcttgcc tgctgctgcc cgcccccgca ctggggctga cgctggcaca
     1561 aaaactggtg accatgttcc atctgatgga tcttagctag ctttacactt tattgttttg
     1621 tctgtggttt ttagtgctgg gcgctattga gtattttgtt ctgcgcttta tctggcgacg
     1681 ctggttctcg ctggcggatt aaacgtcgat tatcgccgta cttcgcggca tactttgctt
     1741 attctccttc gcctaaagga acgtttatgg ataagcaatc actgcacgaa acggcgaaac
     1801 gcctggccct tgagttaccc tttgtcgagc tttgctggcc ttttggcccg gagttcgatg
     1861 tttttaaaat tggcggcaaa atttttatgc tgtcgtcgga gctgcgcggc gtccccttta
     1921 tcaatctgaa gtccgatcca caaaaatccc tgttaaatca gcagatatac ccgagcatta
     1981 agccagggta tcacatgaat aaaaagcact ggatttcggt gtatcccggc gaggaaatct
     2041 ccgaagcgtt acttcgcgat ctgatcaacg attcgtggaa tctggtggtt gatggtctgg
     2101 ctaaacgcga tcaaaaaaga gtgcgtccag gctaaagcgg aaatctatag cgcatttttc
     2161 tcgcttacca tttctcgttg aaccttgtaa tctgctggca cgcaaaatta ctttcacatg
     2221 gagtctttat ggatatcatt tctgtcgcct taaagcgtca ttccactaag gcatttgatg
     2281 ccagtaaaaa acttaccccg gaacaggccg agcagatcaa aactctcctg caatacagcc
     2341 catccagcac caactcccag ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag
     2401 cgcgtgttgc caaatccgct gccggtaatt atgtgttcaa cgaacgtaaa atacttgatg
     2461 cctcgcacgt cgtggtgttc tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg
     2521 ttgttgacca ggaagatgct gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg
     2581 ataaaggtcg caagttcttc gctgatatgc accgtaaaga tctgcatgat gatgcagagt
     2641 ggatggcaaa acaggtttat ctcaacgtcg gtaacttcct gctcggcgtg gcggctctgg
     2701 gtctggacgc ggtacccatc gaaggttttg acgccgccat cctcgatgca gaatttggtc
     2761 tgaaagagaa aggctacacc agtctggtgg ttgttccggt aggtcatcac agcgttgaag
     2821 attttaacgc tacgctgccg aaatctcgtc tgccgcaaaa catcacctta accgaagtgt
     2881 aattctctct tgccgggcat ctgcccggct atttcccctc agattctcct gatttgcata
     2941 accctgtttc agccgtcatc ataggctgct gttgtataaa ggagacgtta tgcaggattt
     3001 aatatcccag gttgaagatt tagcgggtat tgagatcgat cacaccacct cgatggtgat
     3061 gattttcggt attatttttc tgaccgccgt cgtggtgcat attattttgc attgggtggt
     3121 actgcggacc ttcgaaaaac gagccatcgc cagttcacgg ctttggttgc aaatcataac
     3181 ccagaataaa ctcttccacc gtttagcttt taccctgcag gggattatcg tcaatattca
     3241 ggcggtattc tggctgcaaa aaggcaccga agcggcagat attctgacta cctgcgcgca
     3301 gttgtggatc atgatgtatg cgctgctttc agtcttctcg ttgctggatg ttattttgaa
     3361 tctggcgcag aaattcccgg cggcatctca gttaccgctg aaagggatat ttcaggggat
     3421 taaactgatc ggcgcgatcc tggtcggcat tttgatgatc tcgctgctga ttggtcagtc
     3481 gccagcgatt ctgatcagcg gtcttggtgc aatggctgcc gtgctgatgt tggtatttaa
     3541 agatccgatt cttggtctgg tggccggtat tcagctttcc gcgaacgata tgctgaaact
     3601 gggcgactgg ctggagatgc cgaaatacgg cgcagatggc gcggtgatcg atattgggtt
     3661 aaccaccgtc aaagtgcgta actgggacaa taccattacc actattccca cctggtctct
     3721 ggtttctgac tcctttaaaa actggagcgg gatgtcagca tctggcgggc gacgtattaa
     3781 gcgcagtatc agtattgatg tcaccagtat tcgttttctt gatgaagacg aaatgcaacg
     3841 tctgaataaa gcgcatttgt taaagcctta tttaaccagc cgccatcagg aaattaatga
     3901 gtggaatcgc cagcaaggtt ctacggagtc ggtattaaac ctgcgccgaa tgaccaatat
     3961 tggaaccttt cgtgcctatc tgaacgaata tctgcgtaac catccgcgga ttcgtaaaga
     4021 tatgacctta atggtacgcc aactggctcc aggtgataac ggtttaccgc tcgagatcta
     4081 cgcgtttact aacaccgtgg tgtggctgga atatgaaagc attcaggctg atatattcga
     4141 tcacatattt gcgattgtcg aagagtttgg tctgcgactt catcagtcgc caaccggcaa
     4201 tgatattcgc tctctggcgg gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa
     4261 tggatgagag cggacatcgt tttgtcggat gtagcgtgaa tgccttattt ccgacgcagc
     4321 gttttaaatg ccacaaacag gaatacaatc cacaccggca gcaggatcgc tgacaagcgc
     4381 atatcatcca tcgtgcacat cagcagcaaa atcatgccga ggaaggcaat gcagagatag
     4441 tttccgaacg gatagagcag cgccttaaac tgtgtttcac gcccctgacg tcgcatcgct
     4501 gcacgaaaac gcagatgcgc cagacagatc ataatccagt tcaacagcag cgttgctacc
     4561 accagcgcca tcagcagacc aaacgctttt tgcggcagca gatagttgat taacaccacc
     4621 agcgaagtga tcgctccgga aagcatcagc gagttaatcg gcacaccgcg acggctgacg
     4681 cgagtcaaaa acttcggcgc attaccctgc acagaaaggc caaacagcat gcggctgtta
     4741 gagtaaaccc cgctgttata cactgacagc gatgctacca gaatgacgaa gttcagcgca
     4801 gaagctacca cgttgctgtc gagattatgg aaaatcatca caaacgggct actgttggat
     4861 ttcacttcca cccacggata gagcgccagt aaaaccacca gtgaaccgat gtaaaacagc
     4921 aggatgcgat acaccacctg atttaccgct tttggaatgc ttttatccgg atcgcgcgct
     4981 tcagcggcag taatcccaat cagctccaga ccgccgaagg agaacataat taccgccagc
     5041 gacaaaatca gcccattcca gccggtggcg aagaaaccac cgtagcgcca gaggttgtcg
     5101 atactggctt tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa gccgatcata
     5161 ccgatgattg ccagcacttt aatcaacgca aaccagaact cggtttcgcc atataagcgc
     5221 acgttcacca ggttaacggc gttgatgata ataaagaagg cggcagccca aatccacgtt
     5281 ggaacatccg ggaaccagta ctgcatatag atgcccgcag cggtcagctc tgccattccc
     5341 accagcacga acattaccca gtagttccag ccagagagga agcccgcaaa cggtccccag
     5401 tatttatagg caaagtgggc aaatgaaccg gataccggct cttcgaccac catctcgcca
     5461 agctggcgca taatcaggaa agcgatgatc ccggcgacgc cgtaacccag caatacagcc
     5521 ggacccgcca tctgaatcgc cggaccaatg ccaagaaaca gaccagtacc aattgcgcca
     5581 cccagcgcaa tcagttgaat atgacggtta tgtaatccgc gatgaagcgt cggctcttga
     5641 ttcgacgcag tatcttccga tacggttgac gcgtttttca cgcctttccc ctgtgtgtct
     5701 tttttgttga ggggcacctt ttaacattta gtgcccatcg tcgcaagaca caatccacac
     5761 ggttaaacgg ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc cacatcagct
     5821 tatacgccgc cgggataata aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca
     5881 tcggtgcggc aatccggctc atcacctctg aaccagcccc cgttccccac agaatcggca
     5941 gcagaccggc gataatcacc gccaccgtca tcgctttcgg gcgcacgcgc agtaccgcgc
     6001 cgtgatacaa cgcctcatcc agcttctgct cactgaatgt ttgcggatta ttcaacgacg
     6061 gctcggcctc tatggcgtga cgtaaataca tcagcatcac cacgccaaat tcggcggcga
     6121 ccccggcaag ggcaataaaa ccggtgcccg ttgccacaga aagatgaaag cccatccacc
     6181 acagcaacca gatgccgccc accagcgcaa acggtacgct gctgataatc agcaacgctt
     6241 cgcccacccg acggaatgcc agatacaaca gcacgaagat gatcatcagt gtcatcggca
     6301 ccatgagttt cagcttatgg ttggcgcgct ccagtagctc gaactgcccg gagaatgcca
     6361 cgctggtgcc cggttttaac tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca
     6421 ccgacaccat gtcacgatcg cgggcatcaa tataaatcca gctcgtcggg cgcgcattct
     6481 cggttttcag catcgacggt ccggtagaga ctttaacgtc ggccacgtct gccagggtga
     6541 tttgctgctt catcggcgtc aggatcggta gctggcgcag tgcctgcggg ctatcgcgcc
     6601 agctttgcgg ataacgcaga ttaatgggat aacgggcaat cccttccacc gtttcgccaa
     6661 ccatcgcccc gcccaccgca gaagtcacaa acaactgcac atccgccacc gtcataccgt
     6721 aacgcgcggc tttttcacgg ttaatctcaa cgttgatata gcgcccacct tccagacgct
     6781 cggctagagc agaagccacg cttggcaccg ttcgcgctac ttcttcaatt tgctcagcca
     6841 tcgcgtcgat atccgccagc acagtgccgg aaactttaat gccgatgggg cttttaatgc
     6901 cggttgagag catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg
     6961 gcagccgcac ggtgttatcc agttcctcaa tgattttgtc catcgtcatg ccaggccgcc
     7021 actgatcctg cggcttaagc tggatggtcg tttcaaccat ctcgagcgga gcagaatcgg
     7081 tggcggtttc cgctttcccg gttttgccaa atacccgcgc cacttcaggt acgctcataa
     7141 ttagcttgtc ggttttttgc agcatactcg ccgcctctgc tgcggaaatc cccggcagcg
     7201 tcgatggcat atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt
     7261 tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc caccaacagc gtggttttcg
     7321 gccagtgcag tactttcagc aatagaggat gataaacacg aatcaaaaag cgattgagcg
     7381 ggttactgct ttccggcgga attttgccac ggatccagta ccccatcagg atagggatca
     7441 ctacgatcgc cagcagcgcc gcgcccgcca tcgcataggt tttagtgaac gccaacgggc
     7501 caaacagacg cccttcctgc ccttccaggg tgaagatcgg gataaacgac aacgtgataa
     7561 tcagcagact gataaatagc gccggaccca cttcaacaga tgcatcggtg atcacctgcc
     7621 agcgcgtttt attatccagc gtggcgtcag gatgctggtg ctgccactct tccagccgtt
     7681 tatgtgcatt ttcgatcatg acgatagcgg catcgaccat cgccccgacg gcaatcgcaa
     7741 tgccgcccaa cgacataata ttggcattca gtccctggaa gtgcatgaca ataaaagcaa
     7801 tacacaaccc cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa
     7861 acagcgcaca gactaccgcc accacgataa actcttccag caacttgccg ctgaggttgt
     7921 cgatggcgcg gtcaatgagc tggctgcgat cgtatgttgt aactatctcc acgccttccg
     7981 gcagactact tttcagcgtt tccagtttgt ccttcacgga ggcgatcact tctcgggcgt
     8041 ttttgccgga gcgcaggatc accaccccgc ccgccacttc gccttcgcca ttcagttcgg
     8101 caatgccccg gcgcatctcc gggccaatct ggaccttcgc aacatcacgc agataaacgg
     8161 gcacgccatt ttctctggct tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat
     8221 agccgctggc gcgcaccata tattccgctt ccgccagttc gatcgacgaa ccgcccgctt
     8281 cctggtttga agcatccagc gcgcttttta cttcggctag actgatgcca tattgcgcca
     8341 ggcgctgggg atcgataacc acctgatact ctttcaccac gccgcccacc gacgccactt
     8401 ccgcaacgtc agggatggtt tttagctcat atttgagaaa ccagtcctgc aatgagcgta
     8461 aatcggccag atcgtgctta ccgctgcgat ccaccagtgc atattcatag atccagccaa
     8521 cacccgtggc atctggtccc agctcggcgc tgactcccgc aggcagctta ccctgtacct
     8581 ggttgaggta ctccagcacc cgcgaacgcg cccagtacgg atcggtacca tcttcgaaaa
     8641 tgacatacac ataagagtct ccaaactgcg agaaaccgcg cacagtcttc gcgccaggca
     8701 ccgacaacat ggtggtggtt agcggataag tcacctgatt ttcaacgatt tgcggtgcct
     8761 gaccgggata gctggtttta ataatcacct gcacatcgga gagatccggc agcgcatcca
     8821 ctggcgtgtt aatgatggtc caggtgcccc agatgctcag aaacaacgcg cccatcagca
     8881 ccaaaaaacg gttcgccacc gagcgacgaa taatccattc aatcattggt tattccctca
     8941 atgcgcattg gtagcacttt cagagcgcat ccgctccagt gcgccagaaa tattggcttc
     9001 ggaatcaatc aggaacaggc cgctggaaac caccttttca ccttccgcca gaccagagtg
     9061 taacgcggtg acgccttgcg atgcctggaa aacggcaacg cgtttcggta caaagcgtcc
     9121 gtcggcatca acggtaatca cccgctgttc gttgccggta tcaatcagcg cctgtgacgg
     9181 aatgagcagc atcggttcgc tggcggtgtt gagttgcaac caggcgttca tgcccggttt
     9241 tagcgcctcg tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc
     9301 cacgccaggt agcagcgtcc atttgcggat ggtgagtgtt ttatccggtc gcgccggaac
     9361 ggtgagcgta aactgcgagg catctttcac cagccaggcg atagactccg ggatcgcagc
     9421 agtgacccac accgggtcca taccctgaat tttcgctacc acgttatctt tggcgatatt
     9481 cattcccgcg cgcagatcaa acgcggtgat cacgccatca atgggtgctt tgagcgtaaa
     9541 gcgagtctgg attttttgcg tggcgatcag gcggcgaata tccgcctccg gcattcccgc
     9601 cagtcgcagc cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag
     9661 cagtaaatac tcactctgcg cttccaccca gtcaggaatg gtcaggtcga gaagcggtgt
     9721 gcccttttgt actttatcgc ccacggtaag cggatacacc ttgtcgataa acccggcagc
     9781 gcgagcctgc acaatggcat actgatactc gttgtaactg acattcgccg ggaaactctg
     9841 ggcaaaagtc agcggtccgc gcgtgacggt agccgttttc acccccagat tctgcgtctg
     9901 agtcgggtca atgcgcacac cagacgcaga actctcttca tcggcatatt tcggcaccag
     9961 atccatatcc ataaacggcg atttacctgg tttatcgaac cgcgtattgg ggtacatcgg
    10021 gtcgtaccag aataagattt tacgttctgc ggtcgacgtt ttttctgcgg gtggttcagc
    10081 ctttgcaaac caggtaaaac ctgccgcaga aataataccg cccgcgatca tgctgccgat
    10141 aataagcgcg atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg
    10201 taataaagaa aggttgccct gctggacaaa attaaacgcc actttgtcgc cggttttaat
    10261 ttcactcatt ttcgtctgcg gggtgatggt aaagcgcata gtcatctccg gccagttcac
    10321 ggcagcaatc ggatcgtgat ggatggtgat ttttttgctt tccagatcaa taccctttac
    10381 cacgccagtg gcgctaataa cctgcggttg tacttcgctc atggtttcat gatgatgttc
    10441 gttagcctgg gcattaaagc caataacggt aaacagactg aacattgcga cttgcagtgc
    10501 ttttttcatt tattctctcc tggagttaaa aattattgct gccaaccgcc ccctaaggcg
    10561 gtatacaaag aaatttcgtt aacctgacgg gcataattca ggtcgagtaa agtttgtcgg
    10621 gttgcaaata aagaacgctc ggcatccagc acttccagat aacttaccgc gccgtgctga
    10681 tataatgccc gcgcccgttg caaagtaatt tgcagcgacg ccagataacg ctgctgggcg
    10741 ctgatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg
    10801 ttctggattt tctgttcata attcactacc gactgctgct ggcgaatttc ggcgatatcc
    10861 agattggcct ggttgcgtcc ggcattaaaa atggggatct caattttagg aataaaattc
    10921 cacatcccgc tgctagcgtt aaataatgaa gacagatcgc tgctggcggt tgatattccg
    10981 ctggtcaggc tgatagacgg gaaaaatgcc gcacgcgcag caccaatatt agcattagcc
    11041 gccattaacg cgtgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac
    11101 aagcccgccg gtaatttaac gctttgcagg ctgccgctgt ttactgtctg cgcttgcggc
    11161 agtttgccgt agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc
    11221 tgacgtttag cgatgtcact gcgggtactt tctatcaccc cgcgagcctg ttccagcgcc
    11281 agaacattgc tgctaccggt caacagttgt ttttcgacaa acgcatatga ctgctgataa
    11341 ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ccagttgctg attgaaatag
    11401 ctttgcgcga cattagaaac cagcagaata tgcaccgcgc gctgagcttc ctcagtggct
    11461 aaataatttt gtcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa atcgaggtca
    11521 aagctggcgt taaggccggt cgaaaactcc cgtgtcgtgg cggtattccc tttaagatta
    11581 ccgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga
    11641 cgatattgcg cccgtgcctc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc
    11701 accagcgcct cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt gcgccagccc
    11761 acgttctggt agttatctgc tgcgttaacc aagccgttct ggctgagtga gaactgctgc
    11821 ggcacgggca ttgccgtacg ctgataatcc ggtgccagtg aacaaccggt tagcgcaagg
    11881 gccacacaaa atggcagaag tttacaagga gacataggct cataatttct ggtgatttta
    11941 taccgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt
    12001 cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat
    12061 gcggaggaaa aatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga
    12121 ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ctgaatggct
    12181 accatctggc gatgaccggt gattatgacc tgataatcct cgatattatg ctgccggacg
    12241 tgaacggctg ggatatcgtg cgcatgttgc gctccgccaa taaagggatg ccgattctgt
    12301 tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggccgatg
    12361 actatctggt gaagccgttc gcttttgctg aactgctggc gcgggtgcgc actctcctgc
    12421 ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc
    12481 tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac tctgaccagt aaagagttta
    12541 ctctgctgga gttttttctc cgccatcagg gcgaagtgct gccccgctcg cttatcgcct
    12601 cgcaggtgtg ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc
    12661 ggctacgcgg caaaatcgac aacgactttg agccgaagct gattcagacc gtgcgcggcg
    12721 tgggctatat gcttgaggtg ccggatggtc agtaagccat ttcagcgccc gttttcgctg
    12781 gcaacccgtc tgaccttttt tatcagcctg gccaccatcg cggcgttttt cgcctttgca
    12841 tggatcatga tccactcagt gaaagtgcat tttgccgagc aggatattaa tgatttaaaa
    12901 gaaattagcg ccacccttga acgggtactt aatcatcctg acgagacgca agcccgacgc
    12961 ttaatgacgc tggaagatat cgtcagtggt tattccaacg tgttgatttc cctggcagat
    13021 agccacggca aaacggtgta tcactccccc ggtgcgccgg atatccgcga gtttacgcgt
    13081 gacgccatac ccgataaaga cgctcagggt ggcgaggtgt atctcctttc cggcccgacg
    13141 attatgatgc caggccacgg tcacgggcat atggaacaca gcaactggcg gatgattaac
    13201 ttgccggttg gcccgttggt ggacggcaaa ccgatttata cgctctacat cgcgctttcg
    13261 atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat gaccgcatct
    13321 gtcatcagca tcctgatcgt ctttatcgtg ctattggcgg tacataaagg tcacgcgccg
    13381 atccgcagcg tcagccgtca aatccagaat atcacctcga aagatctcga cgttcgcctt
    13441 gacccgcaga ccgagcccat tgagctggaa cagctggtac tgtcgttcaa ccatatgatc
    13501 gagcgtattg aggatgtctt tacccgccag tccaatttct cagcggatat cgctcacgaa
    13561 attcgcacgc cgattaccaa tctcattacg caaacggaaa tcgccctcag ccagtctcgc
    13621 agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac gcgaatggcg
    13681 aaaatggtca gcgatatgct gtttctcgct caggccgata acaaccagct aatccccgaa
    13741 aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt cgaggcgtta
    13801 gcggaagatc gcggcgtgga gttgcgattt gttggcgaca aatgtcaggt tgcgggcgat
    13861 ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcta atgccctgcg ttatacgcca
    13921 accagagaga caattgtagt gcgctgtcag acggtcgatc accaggtgca agttaccgtc
    13981 gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc gattgtttga ccgtttctac
    14041 cgcgttgacc cttcccgcca gcgaaaaggc gaaggtagcg gcattgggct ggcgatagtg
    14101 aaatcgattg ttgtcgcgca taaaggcacg gttgcagtaa cgtcagatgc gcgggggaca
    14161 aggtttgtga ttatgctgcc ggagagagag tgattattaa gttcaaattc agggcgtggt
    14221 gcatcacctc ctggtattgt tgatcaacaa attagagaaa aacttattaa acatatgaaa
    14281 tatcgaaggc ttttattttt caaagtttca cgagatgttt gtatatgaaa cttagttaaa
    14341 tctgtgtttc ttccaattgt agatcacaat gccctttcta aattttcatc gttataaaca
    14401 agccatctat catataaagc aatgaataat ttaatcgcga ttaatcatca ttaattgtta
    14461 tattattcac tcaatctatt tgtcaagtga atttgataaa ttcctaattt aaaatatcca
    14521 ctattgagcc agtatttaac aacgttaaga tgtgcaatgt gcagatcatg ttatcagggt
    14581 ggagcggtag catatatttc ttatgcaatc attcatttta ataatgttaa ttggattatt
    14641 tattgctgtg aataaaaata tattatgaat attggtttaa ctttgcgttt ttgttactcg
    14701 ttgtgatatg cgtacctgtc ctgaataacg ccaagaaaca agaggttgag cttgtgatta
    14761 tttttttgac tgaacagaca cccgtttctg taaagtaata tttaaacaaa attctcacat
    14821 aaccatagca ttttctgttc cttccctaat aacgtctgaa gtcgtcccgc ttatcacatt
    14881 atccgcagtt gtacggtacg gatcattatt cattttactg gaggttgcta tgtcaaccgg
    14941 attacgtttc acactggaag tggacggcct gccgccggac gcttttgcgg tggtttcctt
    15001 tcatctgaac cagtcactct cttcgctctt ttccctcgat ctctccctgg tcagccagca
    15061 gtttctctcc cttgaatttg cgcaggtgct ggacaaaatg gcctacctga cgatatggca
    15121 gggcgatgaa gtacagcgcc gggtgaaagg cgtggtgacc tggtttgaac tcggggagaa
    15181 cgacaaaaac cagatgctgt acagcatgaa ggtgcacccg ccgctgtggc gtgccggtct
    15241 gcgccagaac ttccgtatct tccagaacga ggacatcaaa agcatcctcg gcacgatgtt
    15301 gcaggaaaac ggggtgaccg aatggagtcc gctgttcagc gagccgcatc cttcccgtga
    15361 gttttgtgtc cagtacggtg agactgatta cgatttcctg tgccggatgg cggcggagga
    15421 aggcatcttc ttttatgagg agcatgctta caaaagtacc gaccagagcc tggtgctgtg
    15481 cgacacagtc cgccatctgc ccgaatcttt tgaaatccca tggaacccga acacccgtac
    15541 cgaggtgagc accctctgca tcagccagtt ccgctacagc gcacaaatcc gcccttcttc
    15601 cgtggtgacc aaagactaca cctttaaacg ccccggctgg gccggacgtt ttgaacagga
    15661 aggccagcac caggactacc agcgcacgca gtatgaagtg tatgactacc ccggacgttt
    15721 caagggtgct cacgggcaga actttgcccg ctggcagatg gacggctggc gaaacaatgc
    15781 agaaaccgcg cggggaatga gtcgctcgcc ggagatatgg ccgggacgac gaattgtgct
    15841 gacggggcat ccgcaggcga acctgaaccg ggaatggcag gtggtggcaa gtgaactgca
    15901 cggcgaacag ccacaggcgg tgccaggacg gcagggagcg gggacggcgc tggagaacca
    15961 ttttgcggtg atcccggcag acagaacatg gcgaccacag ccgttgctga aaccgctggt
    16021 cgacggcccg cagagcgctg tcgtgacagg accggcaggc gaggaaatct tctgcgacga
    16081 acatggtcgc gtgcgggtga agttcaactg ggaccgttat aacccggcag accaagacag
    16141 ttcgtgctgg atccgtgtgg cacaggcgtg ggcaggcacc ggttttggcc acctggcgat
    16201 accgcgtgtg ggtcaggaag tgattgtaga cttcctcaac ggcgatccgg accagccgat
    16261 cattatgggg cgcacctacc accaggaaaa ccgccccccc ggcagcctgc cgggaacaaa
    16321 gacgcagatg accatccgct ccaaaacgta catgggcagc ggatttaatg agctgaagtt
    16381 tgatgatgcg acagggagag aacaggtcta catccacgcg cagaagaaca tggataccga
    16441 agtgctcaac gaccgtacca ccaccgtaaa acacgatcac cgcgaaaccg taaaaaatga
    16501 ccagacggtc acgatccagg aaggtaaccg ccttcttacg gtggaaaaag gccacaagat
    16561 caccggagta ctgaaagggt ctttatctga ggatgtcttt caggacagag gcacgattgc
    16621 cggttcggtg catgttgacg ctgtaaacaa tggtggcgaa ggcgacggta tacaggctta
    16681 tacggcgatt aaggaaattt tgctggccgt ggaggaaagc aaaattgcgc tgacgccgga
    16741 tagcattcag ctacaggtcg gggaatcgac ggtaatcagg ctgtcgaagg atggcatcac
    16801 catcgtgggc ggttctgttt tcatcaactg attcactcag attatcaggg agtattttat
    16861 gcagttcact tttaatgaag gccacattca actgccgtct caatggcagg atcagtcaat
    16921 gcaggtcctg gtgtccacag ataacagtgg catcaacctg gtcattaccc gcgaagccgt
    16981 tccacaggga accctgacac cagagctgta ccaggaaacg ctggcgctgt accagggcaa
    17041 acttgatggg tacaccgaac atgcctgccg ggaaatcact ctggcagagg ccccggcctg
    17101 gctgctggat tattcctgga acggtccgga agatgagggg aatcaggggc ggattagcca
    17161 gatagccgta ttccagcggc gtggagatac gctgctgacc ttcactttca gcacctcctt
    17221 atccctgaag aacagtcaaa agacgatgct gctggaggtg ataaagagct tcaccccgtt
    17281 gccaccggag aatgacatcc aaaaggacca gccgcgctga cataaacgag ggataacaca
    17341 tgagtgaagg accaggcggg ccacagggag cgaccgcagg cggtacgctg gcaatgcgaa
    17401 tgctgtcaca gcaggcgatg gtcgccagcc agatgaaacg ggcagccaac gacaaagcca
    17461 ttgcacagat gttggcatca aaaaagtctg gcccccccgc cgccaggctg ggcgatgaaa
    17521 ttcagcacaa gagttttttg ggggcgctgg caggggccgt actgggggcg atagtgacca
    17581 tcgcagaagg ttgcctgatt atggccgcct gtgccaccgg cccttatgcg ctggttctgg
    17641 tacctgcgct gatgtatgcc agctataagg cgagtgatta tgtggaggag aaacagaacc
    17701 agcttgaatc atggataaac agcttttgtg acacggacgg cgccatcaat accggttctg
    17761 aaaacgtaaa aattaacggc gagctggccg cacgtgcagc ggtcaccctt ccccctcctc
    17821 ccccacctgg agcaatacct gaagtcccac agggggaacc ctcatggggt gatattgcca
    17881 ctgacctgct tgaatcggca gcggaaaaag cagtaccact ggcgaaggcc tgggggaacg
    17941 ctgttatcac cctgacggac agcaatgccg gttttatgga tcgcgtcagc gccggcgcat
    18001 cgcttctgtt tcccgccggt ccggtattaa tggagtttgc caccatggtg ggcgggcgtg
    18061 gcgaaatcaa aaaagatgtg gatttcccgg aagccggtga ggacacggcg ctctgcgaca
    18121 aggagaacaa accaccgagg atagcccagg gcagcagcaa cgtctttatc aacaatcagc
    18181 ctgccgcgcg caagggcgac aaactggagt gcagtgcggc gatcgtggga ggttcgccgg
    18241 acgtctttat tggcggtgag caggtcacat atctggatat ccagccggag ttcccgccgt
    18301 ggcagagaat gatcctgggg ggaataacga tagccagcta tcttctgccg ccagcgggac
    18361 tactgggaaa actgaagaat ctggcgagac tcggcaaact gggaaactgc tggggaaaag
    18421 cgggaagctg ctgggcgcaa agctcggcgc gttgctgggg aaaacaggta agtcgttaaa
    18481 aagtattgcc aataaagtca tcagatgggt aacagatcct gtcgatccgg taaccggcgc
    18541 atactgcgac gaacgtaccg acttcaccct gggccagacc ctccccctct ccttcacccg
    18601 tttccacagt tctgtactgc cgctgcatgg cctgaccggc gtgggctgga gcgactcctg
    18661 gagcgaatac gcctgggtgc gtgaacaggg aaaccgggtg gatatcatca gccagggagc
    18721 cacgctgaga tttgccttcg acggtgacag tgatacggcg gttaacccgt atcacgccca
    18781 gtacattctg cgccgccgcg atgattatct ggagctgttc gacagggatg cactgagcag
    18841 ccgcttcttt tatgacgcct ttccgggaat gcgtctgcgc cacccggtga ctgacgatac
    18901 cagcgatgac cgcctggcac acagccccaa tgaccggatg tacatgctgg gcgggatgag
    18961 cgacaccgcc agcaaccgca tcacgtttga gcgcgacagc cagtaccgga tcacgggtgt
    19021 cagtcacacc gacgggatcc ggcttaaact gacgtaccac gccagcggct acctgaaagc
    19081 cattcaccgc acggataacg acatacagac gctggcgacc tacgaacagg atgcgcgggg
    19141 gcggctgaca gaagcggatg cgcggctgga ctaccacctg ttttatgagt acgacgctgc
    19201 ggaccggatc atccgctggt ccgataacga ccagacgtgg agccgtttca cctacgatga
    19261 acagggccgg tgcgtgaatg tcaccggggc ggagggctat tacaacgcca cgctggacta
    19321 tggtgacggc tgcaccaccg tgacggacgg caagggcatt caccgttatt actatgatcc
    19381 tgacggcaat attctgcggg aagaagcgcc ggacggcagc accaccatgt atgaatggga
    19441 tgaattccat cacctgctgg cccgccactc ccctgccggg cgggtggaga aatttgaata
    19501 caacgccgca cacggtcagt taagccgtta cacggcggca gacggcgcgg agtggcagta
    19561 ccgctatgat gagcgcggcc tgctcagcaa catcaccgac cctgccgggc agacgtggac
    19621 acagcagtgt gatgaacgcg gcctgccggt aagtctggtg tcgccacagg gcgaagagac
    19681 ccggctggca tacaccgctc aggggctgct gtcgggaata ttccgccagg atgaacggcg
    19741 tctgggcata gagtacgacc accacaaccg gccggaaaca ctcaccgacg tgatgggccg
    19801 tgaacaccac accgaataca gcggtcacga cctgccagtg aagatgcgcg gccccggcgg
    19861 tcagtcagtg cggttacagt ggcagcagca ccataaactg agcggcattg aacgtgctgg
    19921 aaccggcgcg gaaggatttc gctatgaccg ccacggcaac ctgctggcct ggacggacgg
    19981 caacggtgtt gtctggacga tggaatacgg tccgttcgat tttccggtgg cgcgaacgga
    20041 cggtgaaggc caccgctggc agtaccgcta cgataaagac acgctgcaac tgacagaagt
    20101 catcaacccg cagggtgagt catatcttta tgttctggat aactgcggcc gggtgacgga
    20161 agagcgtgac tggggcggcg tggtctggcg ttaccgctat gacgccgatg gcctctgtac
    20221 cgccaaggtt aacggcctgg aggaaaccat cctctacagc cgggacgccg caggccgcct
    20281 ggcagaaatc atctcgccgg aaggcaaaac gcagtatgcc tatgacaaat ccggcaggct
    20341 gacgggtatc ttcagcccgg acggcacatc acagcgcacc ggctatgacg aacgcgggcg
    20401 ggtgaatgtc accactcagg gccgacgggc cattgaatac aactaccccg atgaacacac
    20461 cgtcatccgc tgtatcctgc caccggaaga tgaacgcgac agacaccccg gtgaatccct
    20521 gctgaaaacc acgtaccgct acaacgccgc cggagaactg acagaggtca ttctgccggg
    20581 ggatgagacg ctgacgttca gccgtgatga ggcgggacgt gaagtgctcc ggcacagtaa
    20641 ccggggtttt gcctgtgaac agggctggaa tgcagccggt cagcctgtca gtcagcgcgc
    20701 cggatttttt ccggcagaag ccacatggga cgggctggtt ccctcactgg tacgggagta
    20761 ccgttacgac agcgcgggta acgtgtcagg cgtcaccagc cgggaagatt acggacggga
    20821 aacacggcgg gagtatcggc tggaccggaa cggccaggtc acggcggtga cagcctcagg
    20881 caccgggctg ggctatggcg aaggcgatga gtcctatggc tatgacagct gcggctacct
    20941 gaaggcgcag tctgcgggca ggcaccggat aagtgaagag actgaccagt atgccggagg
    21001 ccaccggctg aaacaggccg gaaacacgca gtatgactat gacgccgcag gccggatggt
    21061 cagccgcacc agacaccgtg acggctaccg accggaaaca gagcggttcc ggtgggacag
    21121 ccgggaccag ctgaccgggt attgcagcgc acagggtgag ctgtgggaat accgccacga
    21181 cgccagcggc agacgaacag agaaacgctg cgaccggaag aaaatccgct tcacgtatct
    21241 gtgggacggc gacagtattg cggaaatccg ggaataccgc gatgataaac tgtacagcgt
    21301 aaggcacctg gtgtttaacg gctttgagct gataagccag cagttcagcc gggtacggca
    21361 ggcgcatccg tcggtggccc cgcagtgggt gacgcggacg aatcatgcgg tgagcgacct
    21421 gacgggccgc ccgctgatgc tctttaacag tgaaggaaag acggtctggc gaccggggca
    21481 gaccagcctg tgggggctgg cactcagcct gcccgcagac accggctacc cggacccgcg
    21541 cggggaactg gacccggaag ccgaccccgg cctgctgtat gcaggacagt ggcaggatgc
    21601 ggaatccggg ctgtgctata atcggttccg gtattacgag ccggaaaccg gaatgtacct
    21661 ggtgagtgat ccgctggggt tgcagggcgg ggagcagact taccggtatg tgccgaatcc
    21721 ttgtgggtgg gttgatccgc tgggattggc tgcaagttct aaaatcagca gtttgatgga
    21781 ctatattggc gatggtcgtc gtgttagtgg gcatacgggt ttcctggatg gggttcgttt
    21841 atcacgtagt caaataaa
//
LOCUS       DAJTYZ010000067        21817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000067 DAJTYZ010000000
VERSION     DAJTYZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21817
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.067"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            505..1281
                     /gene="exuR"
                     /locus_tag="OO596_002381"
     CDS             505..1281
                     /gene="exuR"
                     /locus_tag="OO596_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6718222.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1626..2288
                     /locus_tag="OO596_002382"
     CDS             1626..2288
                     /locus_tag="OO596_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6718223.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2292..2675
                     /gene="mzrA"
                     /locus_tag="OO596_002383"
     CDS             2292..2675
                     /gene="mzrA"
                     /locus_tag="OO596_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6718224.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2822..3190
                     /locus_tag="OO596_002384"
     CDS             2822..3190
                     /locus_tag="OO596_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR6718225.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3228..3533
                     /locus_tag="OO596_002385"
     CDS             3228..3533
                     /locus_tag="OO596_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6718226.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3536..3940
                     /locus_tag="OO596_002386"
     CDS             3536..3940
                     /locus_tag="OO596_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6718227.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3930..4229
                     /locus_tag="OO596_002387"
     CDS             3930..4229
                     /locus_tag="OO596_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6718228.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4415..4807
                     /locus_tag="OO596_002388"
     CDS             4415..4807
                     /locus_tag="OO596_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6718229.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4877..5890
                     /gene="yqjG"
                     /locus_tag="OO596_002389"
     CDS             4877..5890
                     /gene="yqjG"
                     /locus_tag="OO596_002389"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6718230.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6155..6520
                     /locus_tag="OO596_002390"
     CDS             6155..6520
                     /locus_tag="OO596_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6718231.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6762..7118
                     /locus_tag="OO596_002391"
     CDS             6762..7118
                     /locus_tag="OO596_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6718232.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7169..8065)
                     /gene="yhaJ"
                     /locus_tag="OO596_002392"
                     /pseudo
     CDS             complement(7169..8065)
                     /gene="yhaJ"
                     /locus_tag="OO596_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8170..8871
                     /locus_tag="OO596_002393"
     CDS             8170..8871
                     /locus_tag="OO596_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6718233.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8894..9058
                     /locus_tag="OO596_002394"
     CDS             8894..9058
                     /locus_tag="OO596_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718234.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9192..10502)
                     /locus_tag="OO596_002395"
     CDS             complement(9192..10502)
                     /locus_tag="OO596_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6718235.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10530..11861)
                     /locus_tag="OO596_002396"
     CDS             complement(10530..11861)
                     /locus_tag="OO596_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6718236.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12136..13500)
                     /gene="tdcG"
                     /locus_tag="OO596_002397"
     CDS             complement(12136..13500)
                     /gene="tdcG"
                     /locus_tag="OO596_002397"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6718237.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13572..13961)
                     /locus_tag="OO596_002398"
     CDS             complement(13572..13961)
                     /locus_tag="OO596_002398"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6718238.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13975..16269)
                     /gene="tdcE"
                     /locus_tag="OO596_002399"
     CDS             complement(13975..16269)
                     /gene="tdcE"
                     /locus_tag="OO596_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6718239.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16303..17511)
                     /gene="tdcD"
                     /locus_tag="OO596_002400"
     CDS             complement(16303..17511)
                     /gene="tdcD"
                     /locus_tag="OO596_002400"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6718240.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17537..18868)
                     /gene="tdcC"
                     /locus_tag="OO596_002401"
     CDS             complement(17537..18868)
                     /gene="tdcC"
                     /locus_tag="OO596_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6718241.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18890..19879)
                     /gene="tdcB"
                     /locus_tag="OO596_002402"
     CDS             complement(18890..19879)
                     /gene="tdcB"
                     /locus_tag="OO596_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6718242.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19978..20916)
                     /gene="tdcA"
                     /locus_tag="OO596_002403"
     CDS             complement(19978..20916)
                     /gene="tdcA"
                     /locus_tag="OO596_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6718243.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21105..21449
                     /gene="tdcR"
                     /locus_tag="OO596_002404"
     CDS             21105..21449
                     /gene="tdcR"
                     /locus_tag="OO596_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6718244.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5713 a         5582 c         5113 g         5409 t
ORIGIN      
        1 tgaggcgtcg atggattatc gatacggaat actgtcgcct tgatgtactg aacttcatta
       61 cttttagata ttacgcgaac gttgccttca ccctgactat taatggaaac agccatagga
      121 taaacagtca tatttgactg tgccaaaaaa ggagcgaaca cgaataaaag cggcgtaagc
      181 cgtgataaga gattaatttt cgccaccata taaatttgat cttacaatat tgtttccaaa
      241 aagttggtgt attagagatg tagatttatt gtaagtcaat ataaaaaagc atttttagtt
      301 aatattaaat ttaaaggctg ggaattaact tactgaaatt tagtagcttt cacaatgacg
      361 atacttattt tttaggaatt tataaatatt cacaatatta attcaataca ttaattcaat
      421 caccaaattt atatatgacc aatggcatac ccatgaataa agtggtatga taacttcgtc
      481 cgcattccac tccgggagac gcatatggaa atcactgaac cacgccgttt gtatcaacaa
      541 cttgccgctg acctgaaaga gcgcattgaa cagggcgtct atctggtggg tgataaactg
      601 cctgccgaac gctttattgc cgatgaaaag aacgtcagcc gaacggtagt tcgtgaagcc
      661 atcatcatgc tggaagttga aggctatgtg gaggtgcgta aaggttcagg tattcatgtg
      721 gtttccaacc agccgcgcca tcagcaggcg gctgacaata atatggaatt cgccaattac
      781 ggtccgtttg agttgcttca ggctcgccag ctcatcgaaa gtaatattgc cgagttcgcg
      841 gcaactcagg taacgaaaca agacatcatg aaactgatgg ccattcagga acaggcgcgc
      901 ggcgaacaat gcttccgtga ttccgagtgg gatttacagt tccacattca ggtagccctg
      961 gcgacgcaga actccgccct ggcggccatc gttgaaaaaa tgtggaccca gcgtagccat
     1021 aacccgtact ggaaaaaact acacgaacac attgattccc gtaccgttga taactggtgt
     1081 gatgaccacg atcaaattct caaggcgtta attcgcaaag atccccatgc tgctaagctg
     1141 gcaatgtggc agcatctgga aaacaccaaa atcatgttat ttaacgaaac cagcgacgat
     1201 ttcgagttca atgccgaccg ctatctattc gccgaaaacc cggtagtgca tctcgatacg
     1261 gccaccagtg gcagtaaatg aaactcctgt ccgacaggcg ctgcgacgcg cctgttcgct
     1321 tcgtcggtaa gcgaaaagta taaagtgtca gcctgtgtaa atcctctcgc caccctcccc
     1381 tgcattcagc aaaatcagac tccacggaca tgtaattttg ataacgaaca acgttgacct
     1441 ttgttacaat tagattcaat ttgaatttat gtttttgaat gctttcttat ctcacgattt
     1501 aacagggaat agttcaggct gtgttgatgt atcaaacccg cagaacatac caaaacagca
     1561 ataacattgc ggtagtgcat cttttaaaac cagcgtggcg ttaaccgatt caccaggaat
     1621 aatgaatgga acttttgacc caattgctgc aagccctgtg ggcgcaggat tttgaaaccc
     1681 tggccaatcc atcgatgatt ggcatgttgt attttgtctt gtttgtaatt ttgttccttg
     1741 aaaacggctt gcttccagcg gcctttttac cgggcgacag tttactggta ttggtcggcg
     1801 tgttgattgc gaaaggcgcg atgggttacc cgcaaacgat tctgctgctg accgttgccg
     1861 ccagcctcgg ctgctgggtc agctatattc aggggcgatg gctgggcaat acccgcaccg
     1921 tacaaaactg gctatctcat ttacccgcgc attatcatca acgcgcacac catctttttc
     1981 ataaacacgg tttatcggcg ctgttaattg gtcgctttat tgcgtttgtc agaacactgc
     2041 tgccgacgat tgccgggtta tcagggctga ataacgcgcg ctttcagttt ttcaactgga
     2101 tgagcggtct gctgtgggta ttgatcctga caactctggg ttacatgctc ggcaaaacgc
     2161 cgatattttt aaagtacgag gaccagctga tgtcatgcct gatgctgctc ccggtggtgc
     2221 tgctggtgtt tggcctggca ggttctctgg tcgtgttatg gaaaaagaaa tatggaaatc
     2281 gggggtaagg gatgcaaata cctcgcatgt cgcttcgcca gctagcctgg tccggcactg
     2341 ttttacttct ggtcggcacg ctgttgctgg cctggtccgc ggttcgccag caagagtcta
     2401 cgctggcgat tcgtgccgtt catcaaggca caacgatgcc agacggtttt tcgatctggc
     2461 atcaccttga cgctcatggc attcctttca aaagtatcac ccccaaaaac gacactctgt
     2521 taattacatt tgactccagc gaccagagcg ccgccgcaaa agcggtcctc gacagaacat
     2581 tgccccatgg ctacatcatt gcgcagcagg acaataacag tcaggctatg cagtggctga
     2641 cccggttacg ggataactct catcgcttcg gataacttcc tgttatccga aacatatcac
     2701 tcactttggt gatttcaccg taactgtcta tgattaatga ggcggtggtt aacactaccg
     2761 cttcgttgtc tggacaatcg ttcctttgta ataggtccga atcacaatgg aaggttcaag
     2821 aatgaaatac cgcatcgctt tagctgtttc tctctttgct cttagtgccg gtagttatgc
     2881 cactaccctg tgtcaggaaa aggagcaaaa tatccttaag gagatcagct atgccgaaaa
     2941 acaccaaaac cagaatcgta ttgacggtct gaataaagcc ctgagtgaag tccgggccaa
     3001 ctgttcagat agccagctgc gtgccgatca tcacaagaaa atcgcaaagc agaaagatga
     3061 ggtggcggaa cgccagcaag atttagccga ggcgaagcaa aaaggcgatg ccgataagat
     3121 tgccaaacgc gaacggaaac tggcagaagc gcaggaagag ctgaaaaagc tggaagcgcg
     3181 cgactactaa ctcacaatag tcactactta ctcacctgga gaaaactatg tcgaaagaac
     3241 acactacgga acatctgcgt gctgagttga aatccctttc cgatacgctg gaagaggtgc
     3301 ttagctcatc tggcgagaag tcgaaagaag agttgagtaa gattcgtagc aaagcggagc
     3361 aggcactgaa acagagccgt tatcgcctgg gtgaaaccgg tgatgccatc gccaaacaaa
     3421 cccgtgtcgc ggcggcgcgt gctgatgagt atgtgcgtga aaatccgtgg accggcgtgg
     3481 gaattggcgc tgcaatcggt gtagtgctcg gcgttctgct gtcgcgtcgt taattatggc
     3541 ggacactcat cacgcgcaag ggcccggtaa aagcgttctg ggcatcgggc agcgaattgt
     3601 ttccatcatg gttgaaatgg tggaaacacg tctgcggctg gcggtggtgg agctggaaga
     3661 ggaaaaagct aatctctttc aacttttact gatgctgggc ctgacgatgc ttttcgctgc
     3721 atttggtctt atgagcctga tggtgctaat tatttgggcg gttgatccgc aatatcgcct
     3781 gaatgcgatg attgccacca ccgtggtgtt gctgctactg gcactgattg gcggtatctg
     3841 gacgttacgt aaatcgcgta aatccacctt gctgcgccat acacgccatg agctggcaaa
     3901 cgatcggcaa ctgctcgagg aggagtcccg tgagcagtaa agtcgaacgt gaacgacgta
     3961 aggcgcaact gcttagccag atccagcaac aacggctgga tctttccgcc agtcgtcgtg
     4021 aatggctgga ggcaacaggc gcttacgatc gtcgctggaa tatgctgcta agtctgcgct
     4081 cctgggcgct ggttggcagt agcgtgatgg cgatctggac gattcgccat cctaatatgc
     4141 tggtccgctg ggccagacgc ggttttggcg tatggagcgc ctggcgtctg gttaaaacga
     4201 ccctcaagca gcaacagctt cgcggttaac ttccctctgg ccggagcaat tccggcctta
     4261 tccctcaaat tttttgaaga tttttgacag ttttccttgc taacaatcat cattcaccac
     4321 gtttatgatt ctctccatcg acagcaacga cgctaatacc gcgcccttgc acaaaaaaac
     4381 aatcagcagc ctgagtggcg cagtggagag tatgatgaaa aaattagaag atgttggtgt
     4441 actggtagcg cgcattttaa tgccgattct gtttattacc gcaggctggg gaaaaatcac
     4501 cgcttactct ggtacccaac aatatatgga agcaatgggt gtcccgggtt ttatgctgcc
     4561 gctggtgatt ctgcttgagt tcggtggtgg tctggcaatc ctgttcggtt tcctgactcg
     4621 caccacagcc ctgtttactg cgggctttac gctgctgacg gcatttttat ttcacagcaa
     4681 ctttgctgaa ggcgtcaact cgctgatgtt catgaaaaac ctgacaattt ctggcggatt
     4741 cctgctgctg gcaattaccg gtccgggcgc gtatagcatc gaccgcctgc tgaataaaaa
     4801 gtggtaagca cgcgctatac ttaacgataa aaaacgagga ggaagctcct cgtttttgct
     4861 attggaggag agaaaaatgg gtcaactgat tgacggcgtc tggcatgaca cctggtacga
     4921 taccaaatct accggcggta aatttcaacg ttcagcttcc gcatttcgta actggctcac
     4981 tgccgatggc gcacctggcc ccactggcac aggcggtttt atcgcagaga aagatcgtta
     5041 tcatctctat atttcactcg cctgcccgtg ggcgcaccgc acgctgatca tgcgcaaact
     5101 caaaggactg gaaccgttta tttccgtttc cgtagtgaac ccgctgatgc tggaaaacgg
     5161 ctggaccttt gatgacagtt ttccgggagc aaccggagat acgctctatc aacatgaatt
     5221 tctgtatcag ctttatctcc acgccgatcc acactacagc ggacgagtta ctgttcccgt
     5281 actgtgggac aaaaagaacc acaccatcgt cagcaacgaa tcaacagaaa tcatccgcat
     5341 gttcaatacc gcgtttgatg cgctgggcgc gaaagcgggt gattactacc caccagccct
     5401 gcaaacgaaa attgacgaac ttaacggctg gatttatgac accgttaaca acggcgtgta
     5461 taaagctggt tttgccacca gccagcaagc ctacgacgag gcggtggcga aagtgtttga
     5521 atcgctggcg cgactggagc agattttagg ccagcatcgt tacctgaccg gcaaccagct
     5581 aaccgaagcc gatattcgcc tgtggactac gctggtgcgt tttgatccag tgtatgtgac
     5641 ccacttcaag tgtgataagc accgcatcag cgattacctg aatctgtatg gcttcctgcg
     5701 cgatatctac cagatgccgg ggatcgccga aactgtcaat ttcgatcaca tccgtaatca
     5761 ttacttccgc agccataaga ccatcaaccc tacggggatt atttcaattg gtccgtggca
     5821 ggatctcgat gaaccgcagg acgagatgtt cgcttcggtt aaaaaaaggc accttacgaa
     5881 gtgcctctga taattagcta agtgcatgtt gttcatatcg gatgcggcgt gaacgcctta
     5941 tcaaacctac aaaacattta tattcaatat gttgcaatga ccgcgcaggc cagataagcg
     6001 tagcgcatct ggcgattttg cgcttgtcat cagtcttagc gcgccttcac agcactttat
     6061 taattccttg aacgaatatt tactgccatt tcattcacgt ctattcttaa tttgctgctt
     6121 taaaaacaag tgattgagca acattgaggc aaaaatggac tggtatctga aagtaataaa
     6181 aaattatgtc ggtttccggg gtcgtgcgcg gcgcaaagag tactggatgt ttattctggt
     6241 caacatcatc tttacgttcg tgctggggct gctggataaa atgttaggct ggcaacgcgc
     6301 tggtggcgaa ggcatcctga cgacaatcta cggtattctg gtgtttttac cgtggtgggc
     6361 agttcagttc cgccgcctgc acgacaccga ccgctcggcg tggtgggcac tgctattctt
     6421 aatcccgttt atcggctggc ttattatcat cgtctttaac tgccaggcgg gtacgccagg
     6481 cgaaaaccgc tttggacctg atccaaaact ggaaccttaa aacaaatctg ctcgccggat
     6541 gtctgactca tccggctttt atcatcatgt tgatgaatga ataatttact aatgtttatt
     6601 taaaatattt caatgtcgtt atataaatta cctataaaaa ataaccatgt atttatctta
     6661 aatatcaaaa cctttataac atccgaaaaa gaaagatacg aatagcattt acacaatacc
     6721 cggaatagac tttcgtcact tttaattaaa gggatgtttt tatgcagtgg tatttatccg
     6781 tattaaaaaa ttatgttggt ttctctggtc gcgcacgtcg taaagagtac tggatgttta
     6841 ctctgattaa cgccatcgtc ggcgctatta tcaatgtcat tcaattgatt ttaggtctgg
     6901 agcttccata tctgtctatg ctttatttgc tggcaacctt ccttcccgtc cttgcgcttg
     6961 ctatccgtcg cttacacgat accgaccgtt caggtgcctg ggccttgctc ttttttgttc
     7021 cgtttattgg ctggctcgtg cttctggttt ttttctgcac agaaggtact tctggcagca
     7081 atcgttacgg aaacgatccg aagtttggtt caaattaatc ttagaattgg ggcgatattt
     7141 cgcccctttt tattaacaat aataaatatt attttccgct aaaaagtttg ggaatttccc
     7201 gcagacacca ggatttcgct tcccccatac tgtcacgacg ccaggccata ataatatcga
     7261 tctcactggt cgattccggg ctgacgacac gcaaccgccc ttccgcaata tctttttcga
     7321 ccatcggata cggcatcgtc gccacgccaa gccccgccag taatgcctga cgtttatctt
     7381 caatcgtgct caccgttaag cgcggctgtt tgtccagcag ttgtacggtc aacaccgggc
     7441 gctcacgagc ggtatccgct accgcaattc cacgatattt cacgcgcgtc acttcagata
     7501 acggttccgg ctcctgatga atcgggtgat caggcgcggc aacgtagacg ttcattaacg
     7561 tatagagctt gcgcgagttg atctccgacg aggaacgaaa atgcatatcc ggcgcgataa
     7621 caatatccgc ccgcccctgc tccagtcgtt cctacgcccc cgccagcact tcggtgatga
     7681 ttgccagttg ggtattggct tttgccgcca gtttgtcgat taacgggaaa aaggcaggtg
     7741 tcggtaccag cgcttcggtc acaatggtga gatgcgtttc ccaaccgcgc gcgagggctt
     7801 ccgcatcggt agtcagttta tctgcggctt ccagcaaaac gcgccctcgc tccagcaaca
     7861 tcctcccgac attggtgaat ttggtgcgat ggcccgagcg gtcaaacagc accacatcca
     7921 gctcttcttc cagtttttgc atggtgtagc taagtgcgga aggcacgcgt cccagctcat
     7981 ccgccgccgc cgcaaaactg ccccggcgat cgatcgcatc cataacccgt agtgcttcca
     8041 gcgttaatgc cctttctttg gccatttcgt tctcattcag gaaatttgaa cataccgggc
     8101 agaatatctg gctaacaatg tagcgtccag ccccttacca taaaaggaag taaagagagg
     8161 tcaagaatta tgattactac ccgaactgcc aggcagtgtg gacaagcaga ctacggatgg
     8221 ttgcaggccc ggtatacttt ttcctttgga cactacttcg acccgaaatt gttaggctat
     8281 gcctccctgc gtgtgcttaa ccaggaagtg ctggccccag gtgccgcctt tcagccgcga
     8341 acctatccca aagtcgatat tttaaatgtg attctggatg gagaagcaga gtatcgcgac
     8401 agcgaaggca atcatgttca ggccagcgcc ggtgaggcgt tgctgctctc tacccagccg
     8461 ggtgtcagct acagcgaaca caatctcagc aaagacaaac cgttaacacg aatgcagctt
     8521 tggctggacg cctgcccgca gcgagagaat ccgctgattc aaaaactggc gcttaatatg
     8581 ggcaagcagc aattaatcgc ctcgccagag ggtacgatgg gaagcctgca attacgccag
     8641 caagtgtggc tgcaccatat cgtgctcgac aaaggcgaaa gtgcgaattt ccagttgcat
     8701 gggccacgcg cgtatttgca atcgattcac gggaaatttc atgcgcttac gcatcatgaa
     8761 gagaaagcgg cgctgacctg cggtgatggg gcgtttattc gtgacgaggc taacattacg
     8821 ctggttgccg attccccact gcgcgctttg ctgatagatt tgcctgtcta gttgttttta
     8881 cagggagatg atgatgagta aaaaatcggc caaaaagcgc cagccagtga agcccgtggt
     8941 ggcgaaagaa cctgctcgca ccgccaaaaa ttttggctat gaagagatgt tgagcgagct
     9001 ggaagccatc gtcgcggatg ctgaaacgcg tttagccgag gatgaagcta ccgcgtaaag
     9061 acaggttgcc ggatgcggcg aaaacgcctt atccggccta caaaatccaa caacattaac
     9121 gaattaaaca acccgtaggc cggataaggt gcgccagcat cgcatccggc acaatactca
     9181 aaacctggcg tttatctggc cttgctcgcc ataatctcga taatctgccg atccgtttgc
     9241 tgcatcgaat ggcttgctaa cgcacacagg ttggcaatcg actgctcaac atcatgcgcc
     9301 acgatccctt cattgccggt cacggcggta tcatccagcg ccattaacac cgctttccac
     9361 gcagccgaag cactggtcga aaccttcatc gcgcagctgt tcgacgcacc atcgcaaatc
     9421 atgccgctga catcgccgat catactgctg atcgccatcg agatagtttc ataacgccca
     9481 tccaccagcc atgccatccc ggcggcggcc cccattgctg cggtcgttgc ggcacaaagt
     9541 gcagacaaac gcggtaactg gttatggatg taaattgcgc tcaaatgaga aagcatcagc
     9601 gcacgcgcca gtcgttcatc atccgctccg aagtgttctg ctacaaccac cacaggcatt
     9661 gttgcggtga tcccctggtt acccgagccg gagttactca tagccggaag cgtagcgccg
     9721 cccatacgcg catcggatgc cgcgctggta cgaatcacaa tggatgaaga gagatctttc
     9781 gccagcaaac cgcgctcgca ctgtttttcc agcgtcgcgc caatatgcag cccccactta
     9841 ccgctcaaac cttcctgcga taacgcacaa tttagcttcg cggaatcgag aataaagcgg
     9901 atcgccgcaa acgggacttc attgacgaac ttcaggatct cagccagcgt cgttctggaa
     9961 agcaccgtca gcggagactc ttgctcgccc tctgccacac acgcctgctg ggtaaacacc
    10021 acaccattgt gcgtctcaat acgcacaatg ttggtatgcc cgccgacgat ggtgacacac
    10081 gcccacttct caccgttcca gactttggcg cgtgagaaga ggatttcatt gcaaggttcc
    10141 tggatcttaa cggagacttt ccccgccgcc agcagtgctt tggcatcggc aattgcctgc
    10201 gcagttgcgt ctttcagcac ttccagcccg gcgttggcat ttccacctaa cgcccccagc
    10261 gccgccgcaa tcggcagccc caccattccc gtgccgggaa cggtgacgcc cagaccgttc
    10321 ttcatcagat ttggcgaaac ccaggcttct acacgttcaa ccggaccttc cagttctgcc
    10381 gcagcaaccg ccgccgccag cgccagtgaa atcggttcag tacatcccag cgccggtttt
    10441 acttcctcct gaacggcgag gatgtaacgc tgccataacg gatttaaagt cgaatcaaac
    10501 atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg ccaggaacgg agaaacacaa
    10561 agcaacaaac cagtgacgat aatcaggtac agagacatcc ctttgtattt gtgcaatgcc
    10621 ggtactttgt aaaccagcca cgccgggatc aggcacccta ccatgccgaa aatcgggcta
    10681 cagatagagg tgaagctcaa caccggtgcg ttcagtacga tagcgctcca ggccagcaaa
    10741 atggcgaaaa tcatgatgcc gcgctgaaca agattttcgt taatcttctc ggcaggcatc
    10801 ttgcgacgca ggatgttcat tacgatccct tgcgttgctt cgcgaaagcc taaatagacg
    10861 ccaaagaatg cagtcatgac agcaaagata ttgagaatga cgctgaccac tttcacccac
    10921 gctgcgccgt caccgctaat aaactgtgcg gcaatcgcca gcgcggaaat attctgctcg
    10981 taggctttta ccgcttcgtc atgccccatc gccagcgtga acgacacggc gtagaaaaag
    11041 acggtgacaa acaaaatgcc aaacgcgata ttcattgccc gcaatgcttt atgccgcgcc
    11101 acttcaatag atttttcccg cgagcgataa gagatcacca tcgggcttaa cgtctggata
    11161 aacaaaatcg acgtcagggt aaacggcagc gtaataatgg cgtttttcac cagcagcccc
    11221 agcggcggta gcgaaccgac gttatacaga tgccacattc ccaccatcga cacgcccagc
    11281 gccgcgacca ccagcagctt ggtcagcacc atgccggtcg aaattttgaa taacaatttc
    11341 tcgccgcgtg aagagatcgc caccagaatg caaatcagta ccagaccata aaagggactg
    11401 tctgacagca acccttccgt cacgccgaag gtatgcagat aggaagcact atcgttggtg
    11461 atggcggtgg aatagacgaa catccaaatc accagcatta cgaaatagag cgcacctaac
    11521 aggatgcccc agtttttacc taaataaccg ctaatgacac tcgggtaatc tttacattct
    11581 ggtgattctg ccagcgtatt aataaacaac cgctgaaaca gatacattgc cgggtaacca
    11641 atcaccgatg agagcaaaaa tacccacaat cccatcaaac cgacctgcac cgggagaaaa
    11701 acaatccccg cgccaatcgc catcccgata ctcataatca cccagccggt gtcagtactg
    11761 tcgaatttga tcgcttctcg ccactcgctc tcactcattc cagcacgacc cgccggggtc
    11821 gaagcgtctg caatgacgcc tttattcgat gcgatttcca taatttctcg ctcaatattt
    11881 tgtagggctt attatttttt tccgagccgc atcaaggcga tatgcggtct acgtttttgc
    11941 aggcgagtta ttagaataaa gaaatgatac gcgcgaggtc agagaaaatc ttcacaatca
    12001 agactttgaa ataacggaga tggataagaa ttttctacta aattaatcgc aagagaatat
    12061 tcattctcta tttgcggcag atcacaaaaa aaaggtgcac atttgtgcac ccaaggatga
    12121 aagctgacag caatgtcagc cgcagaccac tttaatggcc agtcctccgc gtgatgtttc
    12181 gcggtattta tcgttcatat ctttgccggt ttcatacatc gtctcgatca ctttatcgag
    12241 tgaaacacgc ggtgccgagg tgcggcgcat cgccatccac gcggcgttta ctgctttcac
    12301 ggcattaatg gcattacgtt caatgcacgg gatttgtacc tgtccggcaa ccggatcgca
    12361 ggtcagccca aggttatgct ccatcgcgat ttccgccgca ttgcatacct gcgccggact
    12421 gccgcccagt agttcagtta accctgccgc cgccattgaa caggccacgc caatctcccc
    12481 ctgacagccg acctccgcgc cagagatgga ggcgttcatt ttatacagcg cgccaatagc
    12541 ccccgctgcc agaaaatagc gggcaattga ccgctcgttt accggacgac ggaacttatc
    12601 gtaataagcc agcactgccg gaataatgcc gcacgcaccg ttagtcggtg ccgttaccac
    12661 gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg tacatgttga tccagtcgat
    12721 gacattcatc ggatcgttag agatgttatc gctagaaacc agctgacgac gcagcgctac
    12781 ggcacgacgc ggcacattga gcggaccagg cagcacgcct tcagtgttca tcccgcgttc
    12841 aataccgtca tgcatcactt gccagatacg ggcaaatcct gcgtcaattt ccgctttgct
    12901 gcgcagcgcc agctcgttgt gcatcatcag gccagatata gacaggccgt tgtaatcaca
    12961 cattttcagc agttcacctg ctgagtggaa atcgtaaggt acggacgttt cgacatcgtg
    13021 cgacaggccg aagtgttctt cttcgacaat aaacccgccg ccgacagagt aataggtttt
    13081 acttaatagc gcttcctgtc ccttccaggc agtgatccgc attccgttct catggcgagg
    13141 caacatttcg ggatggaaga taatgttctt tgctacagga aaatcaacaa tatgcgcacc
    13201 agatgccact ggcagccgcc cgctgcgcgt tactaactct ataaatgcag ggatctcatc
    13261 aatgacaaca tcctgcggac tgtttcctgc cagtcccatg ataatggcga catccgtggc
    13321 atggcctttg cccgtcagtg acaacgaccc atacagatcg accacaatat ggctcgtcgc
    13381 ggttaataag ccgctacttt ccagccgatc aataaaactt tttccggcat tcattggccc
    13441 cacggtatgc gaactggagg gaccaatccc aattttgaaa atatcgaatg cactaatcat
    13501 atccacaccc tcggattgcc gttcagtgaa gtggagcgga acgaccttgc gaccgtcccg
    13561 ctcacgaggc tttacgcact acgtactgcg atagcttcaa tttccagctt cacatctttc
    13621 ggcaaacgcg caacctgcac acagctccgg gtcgggtagg tcgcctgatg ctcatcgaag
    13681 aactgcttat agacttcatt gatggtggca aaatcattca gatcggtgat gaacacggtc
    13741 atcttgatga tatcgcccac gctcagcccg gcagcaacca cgatcgcttt gacgttttcg
    13801 aggcttaaac acgcctgatc ttgcacatca gccgggatct caccggtctg cgggcaaacc
    13861 ggaatttgcc cggaggtgaa gaccatgctg cctaaatcaa cgccctgaac ataagggccg
    13921 attgcgcctg gggcacgttg cgtttcgata atctttttca tacatcctcc ggcgtcagag
    13981 cgcctgggta aaagtacgtg aaataacatc ctgttgctgt tcacgggtca gtgcgttgaa
    14041 gcgcacggcg tagccagaga cacggattgt caggttagga tatttttccg ggtgctcgat
    14101 ggcatccagc agcatttccc gattcattac gttgacgttg aggtgttgac cgccttcgac
    14161 atccgcttcg tggtggaaat acccatccag caggccgaca aggttggttt tacgtactgg
    14221 atcttctttg cccagcgccg caggaacgat tgagaaggtg tacgagatcc catctttggc
    14281 gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc acggcacctt tgcggtcacg
    14341 accatgcatc gggttagcgc ccggcgcgaa cggtgttccg gcgcgacgac cgtccggcgt
    14401 attaccggtt ttctggccgt acaccacgtt agaagtgata gtcagaatcg actgggtagg
    14461 gacggcgttg cgataggttg gcagcgcttt aattttcttc ataaagcgtt caaccaggtc
    14521 gcaggcaatg ctgtctacgc gctcgtcgtt gttgccgtac tgcggatatt caccgtcgat
    14581 ttcaaagtcc accgccaggc cgttttcgtc acggattggt ttcacgcggg catatttgat
    14641 ggcagacagg gagtccgtcg ccaccgacag gcccgcgatg ccgcatgcca tagtgcgata
    14701 gacatcacga tcgtgcagcg ccatcagcga agcttcgtag ctgtacttgt cgtgcatgta
    14761 gtgaatgata ttcagcgcgc tgatgtactg caccgccagc cagtccatga agtgatcgag
    14821 gctgtccatc actttgtcgt aatccagcac gtcgtccatc agcggtgctg ttttcggccc
    14881 gacctgaatc ttcagcttct cgtccacccc gccgttaatt gcgtacagca gtgttttcgc
    14941 caggttagcg cgtgcaccaa agaactgcat ttgcttacca atcaccattg ggctgacgca
    15001 acaagcaatt gcgtaatcgt cgctgttgaa gtcagtacgc atcagatcgt cgttttcata
    15061 ctgcaaggaa gaggtgacga tcgacacctg cgcggcatat tttttgaagg caatcggtaa
    15121 ttcttccgac caaagaatgg tcaggttagg ttccggtgcc ggccccatag tgtgcagggt
    15181 gtgcaaatag cggaaggagt ttttggtcac cagcgtacga ccgtccagcc ccatcccgcc
    15241 gatcacttcc gtcgcccaga ttgggtcgcc ggagaacagc gaatcaaatt ccggcgtacg
    15301 caagaagcgc accatacgga tcttcataat gaagtgatcg atcagttcct gtgcctgctg
    15361 ctcattgagt acgcctgctt taaagtcgcg ctcaatgtag atatcgagga acgacgccgt
    15421 gcgccccagc gacatcgcgc cgccgttctg tgatttcact gccgccagat aagcgaagta
    15481 gagccactgc accgcttcct gcgcattctg cgccgggcga gagatatcaa agccatattt
    15541 cgccgccatt tcctgaatct gcaacagcgc atgacgatgc tctgccagct cctcacgcag
    15601 acggatggtg gcttccagat cttcgccttt ttccagacgg gactggagat cggcaaattg
    15661 cagttcgcgt tcacgcacca gataactgat gccatacagc gctacgcggc gatagtcacc
    15721 gataatgcgc ccacggccat agccatctgg taaaccggtc agcacgccag atttacggca
    15781 gcgcagcata tccggtgagt aaacatcaaa tacgccctgg ttatgggttt tacgcagatc
    15841 ggtaaacaga tattcaaatt cactgtccat ttcacggcca taggcgtgga atgaactttt
    15901 aatcatatta atgccaccga acggatgcag cgcgcgcttt aatggcgcat ccgtttgcag
    15961 accaacaatt ttttccagcg gctggttaat atatcccgca tcatgagcgg taattgtggt
    16021 ggcaatattg gtatcgaaat caaccggcgc gtgggttgcg ttttcgatac ggatgccttc
    16081 cattactttt tcccacaatt ccgtggtggc aggcgtcgct tcggcgagga aagattcatc
    16141 gccttcatac ggtgtatagt tatgttgaat aaaatcgcgg acattaattt cgtttttcca
    16201 gtccgtacct ttaaagccaa gccatgcgtc ggcgtacagc ttatcgctgg tatcaatatc
    16261 tatcttcatg aaaaataatc tctctacaat acttcaacta aattatgcaa attctgcggg
    16321 cgcgttaact ttgcctaaat gaatggcatc caaagcaatc attttttctt cgttagtcgg
    16381 aataacggca caaatgacac gcgcattttc actggaaaca attcgctctc caaaagagtt
    16441 agagcgatta ttcatttctg tatcaatcac tacgcctaat acagccaaat gttccatgac
    16501 cagacgacga attaaacttg aattctctcc tattccgccg gtgaatataa ttccatccag
    16561 gcgacgtaat gaagctgcgt gtccggcaat atgacgggca attcggtgaa caaaggtttt
    16621 aattgccagt tgcgcgcgtt cgtgaccttc atgccaggct ttttccagaa cacgtaaatc
    16681 cgaagaaaga ccggaaatac ctaataatcc cgactcttta ttcactacgc gttccaggtc
    16741 acccaggctc tggttggttt ggctggcgac ccaggacatc gcaccaaagt cgacatcgcc
    16801 actgcgggtt cccatcatca agccttccag cggcgtcatt cccattgaag tatcaacact
    16861 ctgaccgttg cgaaccgcgc agattgacgc gccattgcca agatgcgcca caaccaggcc
    16921 ggagtcatct tccgccagat tcagcagaga atgtgcgcgc tgggaaacat agcggtgcga
    16981 cgtgccgtgg aaaccataac ggcgtacacc taactcttca taatatttcc acggcaggcc
    17041 gtataaataa gcttccggag ccatcgtctg gtggaaactg gtatcaaata ccgccacctg
    17101 agttacgccc ggaaataatt gctgcgccga ttcaatacca cttaaattgg cgtaattatg
    17161 caggggtgcc agtggagaaa cgcgacggat attatcaatg acttcatcgg taataatggc
    17221 ggactcggta aaaatactgc cgccgtgagc gatgcggtgg ccaattaagg ccacactgtc
    17281 atttaaattc cgtttttcca gttcaaatgc aattgccttc aatgcacctt cgtagctgtg
    17341 gtgagccagc ggtgctggct ctcccccatt tacggataag aatgcatttt ccgagttaat
    17401 accgtcggca atacctgaca ttaatacttc acagtcgctg gcatcgagca cggaaaactt
    17461 aatcgaagac gaaccacagt taataaccaa aacaaccgga aattcattca tctcttttct
    17521 catcctgagt tacggattaa aacagtttgt atacgatgtt caggatggtc agcagaccaa
    17581 tcacggtaac aaacacgtta tccagacgac cacggtattt cgccagagac ggcgctttac
    17641 ggatggcata catcggcaac aggcacagca gggatgcgat aatcggtgcg cccatggctt
    17701 caatcaggtc gaggatgttc gggttggcgt aggcaacaac ccaggtggag cccatgatga
    17761 agatcatgct gatagtgttc agtttaccca gcgacacttt agttttgtcg cctttataac
    17821 caaacttcag gaccaggcca ttcagacctt ccagcgttcc cagatagtga ccgaagaaag
    17881 atttgaagat agccacgagt gcgatgatgg aagccgcata ttccagtgta atcgcgaacg
    17941 ttgttttggt accggtcatg gacgcaaagt ggttagccag ataagaaagc actggaatat
    18001 tctgcgcttt ggcttccgcc atgttggccg gagacagcgt aaataggcag ctaaaggcaa
    18061 agaacatcac cactgcaacc atcagcatgc tggcacgaga aatgatttgg gaacatttac
    18121 gttcggtgaa gtcgcgaccg aagtctttct catactcttc acgtttagaa accacgaagg
    18181 aagagacgat tggcgagaaa ttaaaggaga aaaccatgat ggaaatcccc agccagacgg
    18241 tgatcaggat accgtcatga ccggttaacg acagcgaacc gaggtcaacc tggtcgataa
    18301 ctgcagagtt ccagtaaggg atcagcgaca gagaaatcag caccaggctg gcgataaacg
    18361 gccataccag gtagctcatc actttaacca tcagatcctt accaaaccag atgacgaaag
    18421 ccatcagcag cagcaggaac agcgccacaa agccgcgatt cagcggtgca aagccgagct
    18481 ggttttccca gaacgtcata aaggtattgg taatagtaac gccataaatc cacagcagtg
    18541 ggcaaatcgc gaagaagtac aggaacgtga taaccacgcc gccagtttta ccaaaatgct
    18601 cttccaccgt ttccgtaatg ttgccggaag ggttagagcc agaaagacat agacgcgcca
    18661 gcgcccggtg gcaataaaat gcgatggggt atgccaatac caacatcaga agaatcggga
    18721 tcagtccgcc aaaacctgcg cggataggga agaacagcac cccggcgccg attgccgtac
    18781 caaacaagcc taacgtccat gtggtatctg atttacgcca ggacgattgt tttgtctggc
    18841 tggatacaat gctatctgaa gtactcatat cctatcctca acgaattaat taagcgtcaa
    18901 cgaaaccggt gatttgagag acgcgagaaa gatcgatatt gccgccggaa ataatactga
    18961 cggtttttct gttttgaata tattggtcta atttaccgct taataatgca gcacatgcca
    19021 gagcgcctgc gccttcggtg acgactttat tgcgctgaat taaggcaatc atactgtttc
    19081 tgatttcgtc ttcgctgacc agcacgatgt catcgactaa ttcacgaacg atttcgtaag
    19141 ttaaattacc cgggcgggag acatcacaac catccgccag ggtgccggta gttcggtgcg
    19201 tggttatttc tccggagtgg aaagaagccg ccatgccgtg aacgttttca gactgtacgc
    19261 caataacacg aatggtcggg ttaatagatt taattgccac cgcaatacca gcaattaaac
    19321 cgccaccacc aattggcaca atcacgttat cgacatcata gagatcttcc ataatttcca
    19381 gaccaatcgt tccctggcca gcaatcactt tcggatcatc gtaaggtggg ataaaaatac
    19441 ggccttccat ttcgacaatt tcgctcactt tagcgatagt gtcgttgaag ttatcaccat
    19501 gcagaacgac ttctgcggag tagtcgcacg ttgccgctac tttggatttt ggcgcacctt
    19561 ttggcatcac cactttaccg tcgataccca gcatcgcgca ggagagcgaa accccttgcg
    19621 catggttgcc cgcagaacag gccaccacgc ctttgcgttt ttccgcatcg gtcagtgaac
    19681 ttaatttatt aaatgcgcca cgaattttaa atgaacccgt acgctgcata ttttcaaact
    19741 tcaggaatat ttcacctttg caacgttcac taaaatagtt ggagcgaggc atgcctgttt
    19801 tataaattcg cccagccagt cgttgtttcg cttcaataat atcatcaata gcaatcggca
    19861 gatcgtatgt aatatgcatt ataaaacctc ttcgcctgaa ttaaatatgt aggtaaccgt
    19921 aaccgacacc tgcaaaacag acaggtggat tatttatgtt taaataaaaa caaataacta
    19981 accaacttct attaattgcc ttcgtctaca cccattataa gatgaatact ctttggctaa
    20041 ttccaccaaa accgatgctg cttttttaat acgataattt ttcgaccata ccgcggcata
    20101 ttgtgccaca ggtaatgttt cttcaaccgg aatagtaata aattgattag aaccaaaagg
    20161 tgacgtcata tcacaaggaa ttacagttaa gaaatcagca ttgagaacaa gattataaat
    20221 tgtcacgact gagtcggttt taacgatgtt ttcaatactg atgccatttc tttgtaacgt
    20281 agtaagcagt tcgctgtagt accccatatt agtttgtggc aacacccact gttcgttctt
    20341 caacgactcc agcgtggtgg tgccggtgca tgttcgggac ttactggcta ccagcacaaa
    20401 ctcggactcg aacagcggct caacatgtaa atcctgaagc ttcatttctg cacttaatgt
    20461 accaatcgca aaatccagtc gaccgtcgcg gattgccggt aagaacgaag acagttgcgc
    20521 ttcatacata gaaacctgcg ctttcgggaa cacctctttg aacttgttga tcatccctga
    20581 cataaaagta aaaccaatca atgaaggaaa accaaatgag acttccacca ccgcctcaga
    20641 agacataccg cttatctcat taaccatatt tttcatttca cgggtaatgg attcggaacg
    20701 ggagagtaac aattgaccgg caggtgttaa tgttacaccg gtatttttcc gcaccactaa
    20761 ttccacacca aaataatctt caatatcgtt aatgatttta ctgacggccg gttgagttaa
    20821 ccctaattct tttgcagccg agccgataga gccacttcta atgacttcct gaaagactac
    20881 caggtgctgc gttttcggaa gaagaatagt gctcataacg acctacgtta attacctcat
    20941 tgacggcatg aagtgtatca aaatgaaatg aacaggatat gtgcgaccac tcacaaatta
    21001 actttcaata ctttccaggg tatcgtcatt acaattaaat acctttaaat atcaacaagt
    21061 taaagcataa aaatcagcat aaaccctgat ttttgtcaaa aaaaatgacg gggataacca
    21121 tattttatgg cgataacatc attcgttatg tggttaatac aaaaaagggg ctgagaccat
    21181 atttcaagca attaccggat aattaccagg caaaatttga gttaaattta atgagcaagt
    21241 tctcaaattt tattataaat aaaccatttt cagcgataaa tactgcggca cgtcacattt
    21301 tttcacgcta tttgttggag aacaaacatt tattttatca atattttaaa atttcgaata
    21361 caggtattga tcatctcgaa caattgatta acgtcaactt tttctcttct gacaggacgt
    21421 cattttgtga atgcaatcgt tttccataaa ttcttctccc ctcataggcg acgaatagca
    21481 ttttgtgttg aggatcacaa aacgaataat tgctgatcgc cgcgataagg tcagacaaag
    21541 acaacaaggg aaattttcac agagcttttg atcgtcgtag gccacagaat gttgcttcgt
    21601 ttactgtaac gccgggtaaa tgagcgtttt ttgatagtgc gaaagaaccc tgcgaggaaa
    21661 ataagcatct attattgttt gtcatgacga caaggcaggc atcaatgaaa ggcttcccaa
    21721 ttgcacatat ttttcaccct tcaatcccgc caatgcacgc agtggttaac aatcacaata
    21781 gaaatattga ttattggacg gtaaaaagaa agtttgc
//
LOCUS       DAJTYZ010000068        21642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000068 DAJTYZ010000000
VERSION     DAJTYZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.068"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="OO596_002405"
     CDS             complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="OO596_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6718245.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1344..2996
                     /gene="ushA"
                     /locus_tag="OO596_002406"
     CDS             1344..2996
                     /gene="ushA"
                     /locus_tag="OO596_002406"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6718246.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="OO596_002407"
     CDS             complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="OO596_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6718247.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3716..4510)
                     /locus_tag="OO596_002408"
     CDS             complement(3716..4510)
                     /locus_tag="OO596_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6718248.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4648..4989
                     /locus_tag="OO596_002409"
     CDS             4648..4989
                     /locus_tag="OO596_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6718249.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5204..7708)
                     /gene="copA"
                     /locus_tag="OO596_002410"
     CDS             complement(5204..7708)
                     /gene="copA"
                     /locus_tag="OO596_002410"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6718250.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7970..8902
                     /gene="glsA"
                     /locus_tag="OO596_002411"
     CDS             7970..8902
                     /gene="glsA"
                     /locus_tag="OO596_002411"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6718251.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8905..10197
                     /locus_tag="OO596_002412"
     CDS             8905..10197
                     /locus_tag="OO596_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6718252.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10322..10729
                     /gene="cueR"
                     /locus_tag="OO596_002413"
     CDS             10322..10729
                     /gene="cueR"
                     /locus_tag="OO596_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6718253.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10730..11188)
                     /locus_tag="OO596_002414"
     CDS             complement(10730..11188)
                     /locus_tag="OO596_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6718254.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11185..12102)
                     /locus_tag="OO596_002415"
     CDS             complement(11185..12102)
                     /locus_tag="OO596_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6718255.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12248..12925
                     /gene="fetA"
                     /locus_tag="OO596_002416"
     CDS             12248..12925
                     /gene="fetA"
                     /locus_tag="OO596_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6718256.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12912..13691
                     /gene="fetB"
                     /locus_tag="OO596_002417"
     CDS             12912..13691
                     /gene="fetB"
                     /locus_tag="OO596_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6718257.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="OO596_002418"
     CDS             complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="OO596_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6718258.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="OO596_002419"
     CDS             complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="OO596_002419"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6718259.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="OO596_002420"
     CDS             complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="OO596_002420"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR6718260.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16062..16748
                     /locus_tag="OO596_002421"
     CDS             16062..16748
                     /locus_tag="OO596_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6718261.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16745..19159
                     /locus_tag="OO596_002422"
     CDS             16745..19159
                     /locus_tag="OO596_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6718262.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="OO596_002423"
     CDS             complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="OO596_002423"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6718263.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20556..21482)
                     /gene="allS"
                     /locus_tag="OO596_002424"
                     /pseudo
     CDS             complement(20556..21482)
                     /gene="allS"
                     /locus_tag="OO596_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5799 g         5144 t
ORIGIN      
        1 gatcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa gcaaaaccga
       61 aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg tagaccagaa
      121 tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc cccgtccagt
      181 gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata gagccccaaa
      241 tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta cctgccgcaa
      301 cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat ccgaattttt
      361 gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat ttcgagaaaa
      421 tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt ggattgatta
      481 tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa cggctaattt
      541 gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg ttgcctttgc
      601 cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca aagttgccgc
      661 cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata cgggctacgc
      721 gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc gccagcagaa
      781 ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta aagcacatgc
      841 caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc actggttgca
      901 gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata aatgtcagag
      961 aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc atgtcgttca
     1021 gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc ttggtcgttg
     1081 acgcagccgc gcccgccaca ggctggggtt gttcactcat tgccatagga aagtcacttt
     1141 ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact aacatacctg
     1201 tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct ggatgcagat
     1261 ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt tcaacacgtt
     1321 gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg ttagcgctgt
     1381 taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat aaaacctaca
     1441 aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat gaatatggcg
     1501 aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag gttgcggctg
     1561 aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg cccgagtctg
     1621 acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat gacgcgatgg
     1681 cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag gaaaagtggg
     1741 ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag cgcctgttta
     1801 aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg ctgacaaccg
     1861 atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt cgtaagcccg
     1921 ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca gacattatta
     1981 tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac gcaccgggcg
     2041 atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc ggtggtcact
     2101 cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac gtgccgggta
     2161 cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat gagtggggca
     2221 aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg gttaactacc
     2281 agctgattcc ggtgaacctg aagaagaaag tgaccaggga agacgggaaa agcgagcgcg
     2341 tgctgtacac ccctgaaatc gctgaaaacc agcaaatgat ctcgctgtta tcgccgttcc
     2401 agaacaaagg caaagcgcag ttggaagtga aaataggcga aaccaatggt cgtctggaag
     2461 gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt ctggcagccc
     2521 aaatggatcg cactggtgcc gactttgcgg tgatgagtgg aggaggaatt cgtgattcta
     2581 tcgaagcagg cgatatcagc tataaaaacg tactgaaagt gcagccattc ggcaatgtgg
     2641 tggtgtatgc cgacatgatc ggtaaagagg tgattgatta cctgaccgcc gtcgcacaaa
     2701 tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg gcgaaagacg
     2761 gcaaactcaa cgacctgaaa atcaaaggcg aaccggtcga tccggcgaaa acttaccgta
     2821 tggcgacatt aaacttcaat gccaccggcg gtgatggcta tccgcgcctt gataacaaac
     2881 cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat atccagaaaa
     2941 gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg cagtaatccg
     3001 aaagtgccgg atatttgcat ccggcacaat gcttaatcgc ggcgggcgat atcagcaaat
     3061 ttggcatcga ggatcttcgc cagatcgcct gccgccagtt cgatatccag tccgcgcttg
     3121 ccgccagaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat cgtcggcaga
     3181 cgttttttct gccccaatgg gctaatcccc ccaaccaggt atcccgtcga acgctgcgcg
     3241 gccatcggat cggccatctc aactttcttg gcacccagcg cttttgctac ttttttaaga
     3301 tccagttgac ctgcgaccgg cgtaacggcc actgccagat gtttcatatc accgttcact
     3361 gccaccagta gcgttttgta gacttgatcc ggattcaaac ctaatttttt gacgacttca
     3421 tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa cgaaatcttg
     3481 tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta aacaaaaaaa
     3541 cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc taagagtatt
     3601 ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt gacggcataa
     3661 taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca tttttttagc
     3721 gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc gcgaccacat
     3781 atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc tgatgcatca
     3841 gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta ttttgcggag
     3901 gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta tgccagtggg
     3961 tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac atggtaatct
     4021 ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc gcctgcaata
     4081 actggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc gtcgcctgaa
     4141 gaaccatcgc gatttgccac agcggttgag tagaaaagag cgacggagaa atgcccatct
     4201 cctggctaat gtgctgcaga ttttggagtt gctcctcgtt aatgcgctct tccagcgcct
     4261 cgcaagtagg caaattagca aaaggcgtat cgctggtgga aacatccgcc tcgacgatca
     4321 gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc atatcgtggc
     4381 tacccatatg aatactgcca atcagatgaa aatggcgatt gccgggaagg gtgatatcga
     4441 tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt acccggtaca
     4501 acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga gggatggcgc
     4561 aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct tgcatcgcta
     4621 ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc gacgacaccc
     4681 ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa tgagttagca
     4741 gagttgctgc atgttcatcg taatagcgtc agtgcactga tcaataacaa tcgtaaactc
     4801 accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga ttttaggcta
     4861 aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac ccaggaagaa
     4921 ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg tgcaaaaaag
     4981 gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt cacgccgcat
     5041 ccggcatccg agcaccattg cctgatgcga cgcttacccg tcttatcagg cctacaaacc
     5101 tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc atgcgtcgat
     5161 gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc ttcggtttaa
     5221 accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc attgccgctc
     5281 cggcaactac cgggttaagc agtgttcccg taaacggcca caaaataccg gcggcgaccg
     5341 gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg ttgcgcagcg
     5401 ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc atcagggtaa
     5461 tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg acatccgcct
     5521 gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga cgtccttcac
     5581 tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca atcacttcat
     5641 caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg gtcaacatca
     5701 ccagacgata tcccgtttta tgcaggcgtt gcagcgccgc cacgctatca ctacgcaacg
     5761 gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc acaggcgttg
     5821 ctccttgcga tgcctgagca gtaatctccg cttcgatagc tttggtacca acctgttgct
     5881 catttaacag cgcctgattg cccagcaata acgcatgacc ttcagcttca ccgctcacgc
     5941 ccagcccgcg caatgtgcgg aaaccgttga cctgcggcag ctgcatatca cctgctttat
     6001 cgaggatcgc ccgtgccagc ggatggctgg aaccttgctc cagtgccgcc gccagacgca
     6061 atgcctgcgc ttcatcaaca tcagcaaacg ttttcactgc gacaacctgc ggcttccctt
     6121 cggtcagcgt cccggtttta tcgaacacga tggtatcgag tgtactggcg cgttgcagcg
     6181 cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg gaaataatcg
     6241 acatcggcgt cgccagcccc agcgcacacg gacaggcgat aatcagtacc gtggtggcaa
     6301 tcaccagggt ataaacaatt tgcggtgccg gaccaaagaa gtaccagatt gccgcactga
     6361 ccagcgcaat caccaccacc acaggtacaa atacggctga gattttatcc gccagctgac
     6421 cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatccgt gacagcgtgg
     6481 tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc actgtcccgg
     6541 catggacgct ttcgccttcg cctttttgct gtgggattgg ttcgcccgtc agcatcgctt
     6601 catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga tcgccggtcg
     6661 tcaggcgcag taacatccct ggctgaactt ccgccagcgg cacgcttttt tcaccatctg
     6721 cggtgaccac tcgcgccgtc ggtggggtta aatcgagtaa tttctccagc gccttagaag
     6781 aacgctgacg tgcgcgcgct tccagcatat ggccgagatt aatcagaccg ataatcatcg
     6841 cactggcttc atagtaaaga tgccgcgctt ccatcgggaa ccactgcggc cacaggttga
     6901 cgctcatcga atagagccac gccacacccg ttcccagcgc caccagcgta tccatcgtcg
     6961 ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg gcgaaaacca
     7021 tcactgccag ggttatcagg ccgataacta accacaagct gcggttgtca gcggtgacca
     7081 tcatgttatc gccgatcatc ccccagacca ttaccgggat acccacagcc agtgcaacaa
     7141 ttgcctgcca gcggaagcgc ttcatcgtag ctacggcggt ttcttgctgg cgctcgcggc
     7201 gtttagcgtc atcttcaatc gcttccgcac cgtagcccgc tttttccacc gcctgcacta
     7261 aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg tttacccgtg
     7321 cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca cagctggcgc
     7381 agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc gctgccggaa
     7441 gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc agcggtttag
     7501 cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg atcagctgtt
     7561 ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgcc tgctcaacat
     7621 ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag gacaggccgt
     7681 ccagggtcag gtcgatagtt tgtgacataa aacactcctt taagacagtt ttgactggct
     7741 gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa ccaggcgggt
     7801 aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac atttatttct
     7861 ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg aaaatcggca
     7921 ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga tgttagatgc
     7981 aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac ttaacggcgg
     8041 acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg cggcagtggc
     8101 tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc gctttgcact
     8161 ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg gcccgcaggc
     8221 ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag ttatcgcctt
     8281 agagttgcat ggcggcaaac cgctgtcgcc actggtaaat gctggcgcta ttgccaccac
     8341 cagcctgatt aacgctgaaa atgttgaaca acgctggcag cgaattttac atatccaaca
     8401 gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg aacaaacaac
     8461 caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc tctattgtga
     8521 tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca atactgttga
     8581 actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga cgcataaacg
     8641 cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg aagggctgta
     8701 tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg gtgtaggtgg
     8761 cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac caccgctgga
     8821 cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta agcaactcgg
     8881 ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg taacaaaccc
     8941 ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc ggggatcttc
     9001 gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctgggtcgc ctttgctttt
     9061 ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc gagctatccc
     9121 agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt cttttcgcta
     9181 gcgctctcgt tgctgtacct gttgacgctg gcggtgagca tcgccatggt tgcccgtgct
     9241 tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca ccttattttg
     9301 ctctacgcgt tggggatcat tgcggtgatg acgcttttta actccttaag caaccatgcg
     9361 gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt attgctgatt
     9421 attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc ccccagctcc
     9481 ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt tggcatgatg
     9541 gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc gtttctggtg
     9601 gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct tagcgatgta
     9661 tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc ttctccgctg
     9721 ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac ggcttcagcc
     9781 attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag cgaacgcgaa
     9841 ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa cattattgtc
     9901 gtggtgttga ttatgctgat gacggcggcg ctaaatttag gctcactcgc cagtgttgcc
     9961 agcgccacct ttttgatttg ctacctggcg gtgtttgtgg tggcgatccg cctgcgtcat
    10021 gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt gttggtgatc
    10081 gttggcttta tctacagtct gtggtcccag ggtagccgtg cgttgatatg gattattggc
    10141 tcactcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac cgtataacat
    10201 ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg cactggcaaa
    10261 aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg ttgcgcgaac
    10321 gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca ttcgcttcta
    10381 tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc gcacctacac
    10441 gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg gctttaacct
    10501 ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca gcgccgacgt
    10561 caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg agctgcaatc
    10621 catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca gcgccgactg
    10681 cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat taagacgaga
    10741 cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc gtacctgcac
    10801 cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata tgaccgcgcc
    10861 cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc ccgcgctggt
    10921 ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat ttccaccaca
    10981 gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc caacccagcg
    11041 gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac aacaaataac
    11101 catttccgcc cagcatctcg gctgccagca gcaaaccgcc gagactgagc cagaaaatat
    11161 gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc tgtctttcac
    11221 cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg gcatcatcac
    11281 tactttgctg ttactggagg taccgatctg ctgtaacgct tcggtgtatt tctgcgctac
    11341 gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca ttttggtggc
    11401 gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg ccgactgacg
    11461 ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga ggatttccgc
    11521 ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg ctttcatctg
    11581 cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt caatacgggt
    11641 gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc gtgagttgat
    11701 gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg tacggatgtt
    11761 agtcatggtc aggttgatga tcgccagctc cagattgctg acttcataag ccgcgcgcgg
    11821 cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt tatctttcga
    11881 gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct tgcgaccaat
    11941 gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg tatagcgacc
    12001 aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg cgccgacaat
    12061 gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa cctcctgttg
    12121 taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg attactgaaa
    12181 gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc agaaaaaggg
    12241 aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat atctggcggg
    12301 tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat ttaagttaat
    12361 taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt cattgatcag
    12421 cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa agccagaaat
    12481 ctaccgccaa caagtctctt actgcgccca gacaccgacg ctgtttggcg atacggtata
    12541 cgataatctg atctttccct ggcagatccg taaccggcag cctgacccag ccatttttct
    12601 cgattttctc gaacgcttcg ccttgccgga cagcattttg acgaaaaata tcgccgagct
    12661 atctggtggt gaaaaacaac gcatctcact gattcgtaac ctgcaattta tgccgaaggt
    12721 tttattgctg gatgaaatca ccagtgcgct ggatgaaagt aataaacata acgttaatga
    12781 gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga cacacgataa
    12841 agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg gagaaatgca
    12901 ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg gcattaatgc
    12961 tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag aaagatattc
    13021 tctggagcgt cggacgagcg ataattcagc tgattattgt cggctatgtg ctgaagtata
    13081 ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc tgctttaatg
    13141 cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc tcatcgttta
    13201 ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca gggtcgattg
    13261 aatttatccc gatgcaggtg atccctatcg ccgggatgat tgccggtaac gccatggtag
    13321 cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag caacagattc
    13381 aggagaaact gagtcttggt gcgacgccga agcaggcttc agcgatattg attcgcgaca
    13441 gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc ttagtgagtt
    13501 taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag gcgattaaat
    13561 atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc attattgcct
    13621 gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg acgcaattga
    13681 agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg ttcatgtgcc
    13741 gatcaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc gacgccagtg
    13801 catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc tgaccgtctg
    13861 cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct tcattgcgcc
    13921 caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct ggattctccg
    13981 ccacctgctg ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc agcagttcga
    14041 tttgcgccac catcccctgg tagcgggtgt cctgatcctg taacggaatg gttttcagca
    14101 ccgcttctgc atcttcagaa cggttcagcg caatcagcgt ttctgccaac agcaggccga
    14161 tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg gcatcggtga
    14221 aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct tcgcgcggca
    14281 gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg aagccatcta
    14341 ccggttgccc gttctggaac agatacaccg tcggaatcgc gcgcagacca aactgggcgg
    14401 caatcatctg ctccgcatcg cagtccagct tcgccagaat aaattgcccg ttgtactgcg
    14461 ccgcaaggct ttccagaatt ggggttaact gcaaacagtg ctggctacgt tcagaccaaa
    14521 aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg ttagattcgt
    14581 taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat attttctttg
    14641 acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc atcacgcgcc
    14701 ccggcagcag gcgcttaagc accattactg cccaagtcac cagcgttacc ggatagcgca
    14761 tcttcggctt ctcgctaata aaagcatggc gtactttgtc caccaccgct tccggtccca
    14821 acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc gtctggttga
    14881 cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact ttaattccgc
    14941 tgtggcgtag ctccatgcgc agcgcgtctg accatgcctc cagcgcatat ttactggccg
    15001 cgtaagcgcc acgacccggt gtggagatta atcccatcac cgatgatgtc atcacaatac
    15061 gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg tgtgcgccga
    15121 aaaagttggc ggaaaactgc tgttccatct gcgcgcggct gatggtggaa agggggccat
    15181 acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc agggcgatca
    15241 cttcgtcggc tgcgcgatca acactttctg gcgaatccag atcgatcaac acgccggtaa
    15301 atcccatgct gttcatacgc tcaacatcat ccggtttccg gcaacctgcc agcacatgaa
    15361 aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa catccggtaa
    15421 ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta tgagtcatga
    15481 tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg ggcgtcgcgg
    15541 ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc ttccataaaa
    15601 aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc gctaaaggct
    15661 tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa tggttcagcg
    15721 ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg ctgtggctga
    15781 aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccaacgcgg ctgatgctgt
    15841 ttcagcagag ccggaaggcg cgccagtcct tgttgcgagg tgtcgccgct gatgctggca
    15901 ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca ggccgcgctg
    15961 gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt gtccgctgcg
    16021 gcggcacgga aggttaacag ggccaggaac aggaagggca aatgccagcg gaaaacattg
    16081 ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc tccatcctca
    16141 ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc gagtcgggat
    16201 cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc agtggcgaag
    16261 tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca aagttgcgcg
    16321 cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt aacgcgctgg
    16381 aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt cgtaacgggg
    16441 cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt ccggcacagc
    16501 tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt cgacctgatg
    16561 tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat aaaattgccg
    16621 acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg acccacgacc
    16681 tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag ttgcaggagg
    16741 aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat taattgtctg
    16801 gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat
    16861 ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg cgttgcgcag
    16921 ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg
    16981 caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc tggcgaacgt
    17041 caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc cccctggcct
    17101 gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc ttaacctgaa
    17161 aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag aagtgattca
    17221 ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga tgaatctggc
    17281 ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt
    17341 cggcggcaac gagaaccagc tcgacggcta tgataaatgg ttgttacctc agcttaaacc
    17401 cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat cgatggaacg
    17461 ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag tggcagcggt
    17521 ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga tcctcaaaac
    17581 gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct
    17641 gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg tgttgatggt
    17701 gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc cgtggctgtg
    17761 ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat accgcttgtt
    17821 gctggcaacg cagcctttac gcgtattacg taatgatgtg gtagcgaacg tctggccgct
    17881 gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg
    17941 tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt tgctgtgcgg
    18001 tgtgctgggc tggatgcagc tgaatgtact tcgccgcatg acgctgaaat cgctacctct
    18061 gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc agctttcggc
    18121 attttcgctc tcctttatgc tgctggcgct gttgctggta ttgcgtggcg atctgctcga
    18181 ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta acatcgccac
    18241 agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc cggaatcatt
    18301 ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag aaggtaatga
    18361 agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc ccgatcataa
    18421 tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct
    18481 ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg atacccagga
    18541 gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt
    18601 ctattttatt ttcccggaag gggcattaga cgggcaaccg cagagctggc ttaccagttt
    18661 ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc cgaccattag
    18721 cctgttagat attggcgcga ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg
    18781 ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt tgctggcaca
    18841 ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca cactcggtgc
    18901 ggggaaaaaa ctgctgcgta ccacgttgtg gtgtgagttc gccatgcttg ggtttgtttc
    18961 tggcctggta gccgcaattg gtgcggaaac ggcgctggca gtgttgcagg cgaaagtgtt
    19021 tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca gcggtgcact
    19081 gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta aggcgctgtt
    19141 caggcagttt gcggggtgat gacaggtgtt agattgcctg atgcgctacg cttatcaggc
    19201 ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat aaggcgttta
    19261 cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca actgtcatgt
    19321 cggtttttta tatctgtatt ctggtgcaac cattctgttc tcacgacaat cgccgtttca
    19381 gaataccgcc catcactgcg tcttaaccca ttccgccact tccgcccact caccgcgaaa
    19441 gacaactttt tccgcttttt tctcaagctg atagcgatac atcgggtcgt aatattcttt
    19501 aagtaacggc accagccagg ccagatgacc gtcggtgctg tcggtggtga gttgcgttgt
    19561 cagcgccgta tccagctggg cagcgagttc gttatagcgc tgtaacccca gccgacgctt
    19621 aatagccgaa agtccgtgat gcaggtattc gcaatattcc tgccagccct gttcgtcgcc
    19681 gtacgcgtgg gtaaaatcat gatgcatacg caagaaatac tcttcgttca ggcgctcaag
    19741 acggatctca aacggatctt ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag
    19801 gcattccggc aggtgattcg aaccgatcat ccggctttcg tcttccagca cccacaggcg
    19861 caaatcctga cgggcgtcgg tttttagcat ttcggcagcc agcaggtttt caaaactcgc
    19921 ctggctaagt tgtggttgta aggtgcgacc aaacgccgaa ccgcgatgac gcgctaaccc
    19981 ttccagatca acaccgttcg gctgttgctg cactaacagc gttttaccgc tgccggtaca
    20041 accgccaatc agcactatcg gtttttgtgc cagttcaata gtcgcctgaa tcgcggcttg
    20101 gcgcagtgcc ttataaccgc cttccaccag cggataatca atccccgctg catacaacca
    20161 gctttgcaca atatgtgagc gctgaccgcc acgggcgcag cagagaatac cttgcggatt
    20221 ttgcaggcac gctgcccgcc aggcgtccat gcgctgctga cgaatttcac ccgccaccag
    20281 tttatgcccc agcgccagcg ccgcgtctga gccttgctgt ttataacaag tgccaacggc
    20341 ggcgcgttca tcgttattca ttaacggcag gttgatagcg gcgggcattg cgccttgctc
    20401 aaactcgata ggggcgcgaa catcaataat gggcgtatca gcaatcagca gggcacgata
    20461 gtcctgttcc gtgtgtctct cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc
    20521 tattttacgc gccaacgcgc aaggaaactt gatttttaac tgcgtgggtt gccgaaaatt
    20581 tctaaaaatc cgctgatttc cggcctgcgc tgggtaaaca aggtcacaat atcttctacc
    20641 gctttgccgc tgccaaattt gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt
    20701 gggattaccc ggctgaccag ttgttgatta tcgatcattg actggcaaag tgattttggc
    20761 aaaaaaccaa tgccaacacc cgccagatgg gcggcgattt tcgtttccat atcgggaaca
    20821 ataatctctt tttgccccgg caatcgccag gcgacgcgtt tggttaaggt tcgggcgctg
    20881 tcttcaatat tgaccgccgg aaagcgccgc aactgcgctt ctgttagcgg ctcttcaacg
    20941 ttcgccagcg gatgatccgc cgccatgaca aagcgccatt gcaccgatcc taagggatca
    21001 agactaaagg tatttgccag cgcctcagtt cccgtgaccc cgatagccag cgaaaaacct
    21061 tcgtacaata gcgagtccca gacgcccata tagatttgtc gggagatgtg aaactgggta
    21121 aaggggtaac gctcattcag ccacgccagc aactgggcga cggcctgggg gttatagagc
    21181 aggttgttga tgacaatatt cacctagcgt tccacgccat cattcacctg ttgcagctcg
    21241 ctgggcatac tttccagcca gctcagccag tctctggcct gggaaagtag atgctcgcca
    21301 gccgctgtca acgtcacgct gcgagtcgtg cggaaaaaca gcgccactcc ggtattctct
    21361 tccagaagtt taatgcgata actgatcgtc gccgtggttt tacataatcg ttctgccgct
    21421 tttgaaaaac ttcctgtttc agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac
    21481 atcttcaggt atcccctttt aaatccgcaa gttgcgtgat tttcttatcc tctgatttat
    21541 cagtattttt acatgataac cctgttcaat ttgtggacta aatctagttt tggaaaaata
    21601 ttccaacttt tgtattgatg ttgttctctt aaggttttag at
//
LOCUS       DAJTYZ010000069        21373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000069 DAJTYZ010000000
VERSION     DAJTYZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.069"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1365
                     /gene="espL1"
                     /locus_tag="OO596_002425"
                     /pseudo
     CDS             1..1365
                     /gene="espL1"
                     /locus_tag="OO596_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1537..1644
                     /locus_tag="OO596_002426"
     CDS             1537..1644
                     /locus_tag="OO596_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718264.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1697..2455)
                     /locus_tag="OO596_002427"
     CDS             complement(1697..2455)
                     /locus_tag="OO596_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6718265.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2742..3671
                     /gene="pfkB"
                     /locus_tag="OO596_002428"
     CDS             2742..3671
                     /gene="pfkB"
                     /locus_tag="OO596_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6718266.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3772..4062
                     /gene="ghoS"
                     /locus_tag="OO596_002429"
     CDS             3772..4062
                     /gene="ghoS"
                     /locus_tag="OO596_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6718267.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            4168..5028
                     /locus_tag="OO596_002430"
     CDS             4168..5028
                     /locus_tag="OO596_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6718268.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5069..5605)
                     /locus_tag="OO596_002431"
     CDS             complement(5069..5605)
                     /locus_tag="OO596_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6718269.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5752..6420
                     /gene="hxpB"
                     /locus_tag="OO596_002432"
     CDS             5752..6420
                     /gene="hxpB"
                     /locus_tag="OO596_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6718270.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6583..7173
                     /locus_tag="OO596_002433"
     CDS             6583..7173
                     /locus_tag="OO596_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6718271.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            7306..8697
                     /gene="tcyP"
                     /locus_tag="OO596_002434"
     CDS             7306..8697
                     /gene="tcyP"
                     /locus_tag="OO596_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR6718272.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8701..9517)
                     /locus_tag="OO596_002435"
                     /pseudo
     CDS             complement(8701..9517)
                     /locus_tag="OO596_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9794..10057)
                     /gene="cedA"
                     /locus_tag="OO596_002436"
     CDS             complement(9794..10057)
                     /gene="cedA"
                     /locus_tag="OO596_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6718273.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            10240..12501
                     /gene="katE"
                     /locus_tag="OO596_002437"
     CDS             10240..12501
                     /gene="katE"
                     /locus_tag="OO596_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6718274.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(12759..13517)
                     /gene="chbG"
                     /locus_tag="OO596_002438"
     CDS             complement(12759..13517)
                     /gene="chbG"
                     /locus_tag="OO596_002438"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6718275.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(13530..14882)
                     /gene="celF"
                     /locus_tag="OO596_002439"
                     /gene_synonym="chbF"
     CDS             complement(13530..14882)
                     /gene="celF"
                     /locus_tag="OO596_002439"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6718276.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14987..15829)
                     /gene="chbR"
                     /locus_tag="OO596_002440"
     CDS             complement(14987..15829)
                     /gene="chbR"
                     /locus_tag="OO596_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR6718277.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(15837..16187)
                     /gene="chbA"
                     /locus_tag="OO596_002441"
     CDS             complement(15837..16187)
                     /gene="chbA"
                     /locus_tag="OO596_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6718278.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(16238..17596)
                     /gene="chbC"
                     /locus_tag="OO596_002442"
     CDS             complement(16238..17596)
                     /gene="chbC"
                     /locus_tag="OO596_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR6718279.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(17681..18001)
                     /gene="chbB"
                     /locus_tag="OO596_002443"
     CDS             complement(17681..18001)
                     /gene="chbB"
                     /locus_tag="OO596_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6718280.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(18300..18638)
                     /gene="osmE"
                     /locus_tag="OO596_002444"
     CDS             complement(18300..18638)
                     /gene="osmE"
                     /locus_tag="OO596_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6718281.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18840..19667
                     /gene="nadE"
                     /locus_tag="OO596_002445"
     CDS             18840..19667
                     /gene="nadE"
                     /locus_tag="OO596_002445"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6718282.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            19897..20784
                     /gene="cho"
                     /locus_tag="OO596_002446"
     CDS             19897..20784
                     /gene="cho"
                     /locus_tag="OO596_002446"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6718283.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20744..21319)
                     /gene="ves"
                     /locus_tag="OO596_002447"
     CDS             complement(20744..21319)
                     /gene="ves"
                     /locus_tag="OO596_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6718284.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         5692 a         5280 c         4893 g         5508 t
ORIGIN      
        1 cccttgattc attgagaaat ggaaaaggca aagtcaacct gattaaacac tactcctccg
       61 ttgaatccat acagcagcat gtccccttag tccgggacgc ggagttcaga gcattacttc
      121 gccatcctcc tgcagggagt cgcgttatcg cgagtaagga ttttggcttc gctttagata
      181 ttttcttttg tcgaatgatg gcaaacaatg tcagtcatat gtccgcgatt ttatatatag
      241 acaatcatac tttgtcagta aggctacgaa taaagcagtc agtgtatgga caattaaatt
      301 atgttgtgtc tgtttacgac ccgaacgata ccaacgttgc cgtcagaggc acccacaggg
      361 cagcacgggg ctttctctcg cttgataagt tcatcagttc agctcccgat gctcagacct
      421 gggctgatag gtatgttcgc aactgtgcaa ttgctattct tcctctatta cctgagggag
      481 ttccagttgc tattttagcg ggtatcacga cacgaatgcc atttgcccct atacatccat
      541 cggcaatgtt gttaataatg gccacaggcc agactcaata gcttattaca ctattcaaac
      601 agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag aatagcgttg
      661 gtacacctgc tttatttctg gctatgatga acggacaaac tgacaacgta aaaatattta
      721 tgcaagaaat tcagtcactg gtatacaatc atatcattca tgaagataat ctggttaaat
      781 tactgcaaac taaaagtgct aacgaaacac ctggactcta tatctccatg ttgtatggat
      841 tcgatgaaat aatcgatatc tttctgaatg cattaaccac tccaatagca caagaacttt
      901 taaacaaaaa aaatggtgat gagtatttta gccatgaaaa tacatgatgg tgagccagga
      961 ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct ctctaaaatt
     1021 aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt attaaaagga
     1081 gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa tgaagacgtc
     1141 gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt tagtcaacat
     1201 cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt tcatatagcc
     1261 attcatcata agcattataa aactgtagaa acatattatg ctgctataaa tgtcatcagc
     1321 caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc tatttacgat
     1381 atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac catgtgcaca
     1441 acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctatccctt ttgttacact
     1501 ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg ctttgataaa
     1561 aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt gttgctacgt
     1621 tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac cgcataacac
     1681 ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga cgatcggtat
     1741 gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc accttcagtc
     1801 caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt gtatcttccg
     1861 cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc catgcataag
     1921 ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat ttgtcgtaac
     1981 gcacacctgg gccgaattca aagcggaaac tgtgtaccgg gccattcaaa aactggcgac
     2041 cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct gtcagccagc
     2101 ttgcctgacc aaataaatag tcataatcag ttaaattaaa acggctacgc ccgcccgccg
     2161 catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc ccccacagcg
     2221 accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt gaggagcttt
     2281 tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa ggttttttag
     2341 cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca ttcagtgacg
     2401 caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc ttcatttatc
     2461 aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct taaggatcaa
     2521 agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact tagcaatgta
     2581 ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt atatatattt
     2641 atctgcaaaa ttttaaataa agctccaata aatcatattg ttaatttctt cactttccgc
     2701 tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt atctatacgt
     2761 tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat cccgaaggaa
     2821 aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac gtcgcccgcg
     2881 ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc gcgaccggcg
     2941 aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta gaagccaaag
     3001 actggacccg gcagaattta catgtacatg tggaagcaag cggtgagcag tatcgttttg
     3061 ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag caagttctgg
     3121 aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt gtgaagctgg
     3181 aaaaattaac ccaactgatt tccgcttcgc aaaaacaagg gatccgctgc atcgtcgaca
     3241 gttctggcga agcgttaagt gcagcactgg caattggtaa catcgagttg gttaagccta
     3301 accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccggac gatgtccgca
     3361 aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt tcccttggtc
     3421 cacaaggagc gctgggtgtt gatagtgaaa actgcattca ggtggtgcca ccaccggtga
     3481 aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca ctgaaactgg
     3541 cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg agtgcagcca
     3601 cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt tacgcttacc
     3661 tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac ctgtcggcaa
     3721 cgcgtttctc cgactttgct caaaagtcat gtgataacaa aggggtgaac tatggccagt
     3781 ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt gactgaaatt
     3841 aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac ggatgatgag
     3901 ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca aagtgaagaa
     3961 gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga tcctgaaatc
     4021 accatcacga cttgggagga atggaatagc aacagaaaat aaatggtttt tgggcaataa
     4081 tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct aaccttatga
     4141 tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct tttgagcgag
     4201 cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga agtccatgcc
     4261 gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga aagggaactg
     4321 cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag taaaaccgtc
     4381 accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct ggtgatggat
     4441 tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca gcaaattgcg
     4501 cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa ctcgctctcc
     4561 acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc tgaacaacgg
     4621 attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa tatcgacgcc
     4681 atcgtcgagc atattcagca gcgtctggcc tcgcatcaac tgcagccttc tctgttgcac
     4741 ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat tttcgaccct
     4801 gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca tactgaacag
     4861 ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga tttccttgaa
     4921 cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt tggcggtcag
     4981 catttggtta ttgctcagca gtcattggat agattattag cagcatgata tgggttgagg
     5041 ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa aagaacacat
     5101 aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc aggaaaatag
     5161 tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc tcttcccctt
     5221 tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc gcctggcgac
     5281 tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg aaaatcaccc
     5341 agaacataac gttcacgcca ccattaaaat taggggtcgg ggagttatac cagaacagat
     5401 tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag tcgagcatga
     5461 ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc aaagaaatca
     5521 atgtggagat caatgcaggt ataaaaatca cccaacccaa aatccttttc aaaacagcaa
     5581 tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc gtttcactta
     5641 gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata ggcgcttaat
     5701 cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt aatgtcaacc
     5761 ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga ctcagaacct
     5821 ttacgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat ctcccgtcgt
     5881 aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct ttggtacgcc
     5941 cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat tgcccgtgcc
     6001 atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc tgttgcgtta
     6061 tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca tatgctggaa
     6121 aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc ggctgaaaaa
     6181 ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa actgggcgtt
     6241 gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc ttctaaagca
     6301 gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc acgttttgta
     6361 ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct tctcggttaa
     6421 tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca ttcaactgac
     6481 ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg cactgtataa
     6541 aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc ggaaggtcac
     6601 cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac gcccgtgctg
     6661 gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt gttaccggac
     6721 atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa accgatcgcc
     6781 cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc gctgctggca
     6841 accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct acaaggaatg
     6901 gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg tgttcccctg
     6961 ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa gggcaaccaa
     7021 ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc ccattcatta
     7081 cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca gatccagttt
     7141 catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct ttctggaata
     7201 agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc gccaacacta
     7261 tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa ctttccatta
     7321 attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac ccgccataaa
     7381 cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt ttttggcctt
     7441 gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt acagtggttt
     7501 aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc gttagtcttc
     7561 gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg caaaatcagt
     7621 tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt cggtgtgctg
     7681 gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc agaaactgca
     7741 cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag cgttccgcag
     7801 ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc caatccgacg
     7861 tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt gaaactgctg
     7921 aaggatgatg cgccgaaagg tgaacgcgtc ttagccgcta tcgataccct acaaagctgg
     7981 gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct ggctctaatg
     8041 accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag tttcgttgtc
     8101 gcgtcctacc tcggtctgct gattatgttt gcagtgcatg gcattctgct gggcattaat
     8161 ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt tgcctttacc
     8221 agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg tcgtctgggc
     8281 gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg tcagaacggc
     8341 tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt tggcattaac
     8401 ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag ttccgcaggc
     8461 gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc tgcgatgggc
     8521 ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga catgggccgt
     8581 acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca gtggctgaag
     8641 caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca ccgttaattc
     8701 ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta aatgttccat
     8761 cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa tgtccaactt
     8821 atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa cctcataccc
     8881 gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca caccacttcc
     8941 cattcccgga agatttagtg gattaaaaag aagattgata ttaagcattg atattatcga
     9001 aggaaacttt aggagactct catttttttt gaatcgcagc aaaaatgctc ccggcaggat
     9061 gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac ctatttttta
     9121 tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc atcccaacaa
     9181 aattctcatt aatatctctt atccgaaact cactgccata aagattatta attatcactc
     9241 tatatttaac attgtcctgc agatacgtat caaaactaag tgctggagct cctggctttg
     9301 aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc gactctgcta
     9361 tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc atggtagact
     9421 caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca ccataattac
     9481 tctgtattaa gtttgtagaa aacatctccc gccccatatt gttaacaaaa ttattatctc
     9541 atttaaatct aagtcactta caatataagt ttaagagcga cgccacagga tgaactatca
     9601 aaaatagctc atcatgatta gcaaaactta accattttaa aataaataaa caattaaaga
     9661 aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt acaggtggtt
     9721 tgaatgaatg tagcaacgaa atgcagaatt tcaggtcatg taactcccgg caaaaccggg
     9781 aagtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt gcccaacgtt
     9841 gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc atcagtacaa
     9901 acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc tttatcgcat
     9961 gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg ttttgctggc
    10021 ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga aaaactgtag
    10081 tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca ccgtttccag
    10141 aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt ttgaccaaaa
    10201 cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca taacgaaaag
    10261 aacctacatc agcaccagtc accactacac gattccagcg aagcgaaacc ggggatggac
    10321 tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc gccaggtgca
    10381 caacctaccg cccctgggag cctgaaagcc cctgatacac gtaacgaaaa acttaattct
    10441 ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca gggcgtgcgc
    10501 atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct gctggaagat
    10561 tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga acgtattgtt
    10621 catgcacgcg gatcagcagc tcacggttat ttccagccat ataaaagctt aagcgatatc
    10681 accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt taggttctct
    10741 accgttcagg gtggcgctgg ctctgcagat accgtgcgtg atatccgtgg ttttgccacc
    10801 aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc aatcttcttt
    10861 atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga accgcactgg
    10921 gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc tctgcaacct
    10981 gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg cagttaccgc
    11041 accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg gaaggcaacg
    11101 tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg ggatgaagca
    11161 caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga agccattgaa
    11221 gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga agatgaattc
    11281 aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact ggtgcccgtt
    11341 cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc cgaaaacgaa
    11401 caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa cgatccgctg
    11461 ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg tgggccgaat
    11521 ttccatgaga ttccgattaa ccgtccgacc tgcccttacc acaacttcca gcgtgatggc
    11581 atgcatcgta tggggatcga cactaacccg gcgaattacg aaccgaactc gatcaacgat
    11641 aactggccgc gcgaaacacc gccgggaccg aaacgcggcg gttttgaatc atatcaggaa
    11701 cgcgtggaag gaaataaagt tcgtgagcgc agtccatcgt ttggcgaata ttattcccat
    11761 ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt cgatggtttc
    11821 agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt tgaccagctg
    11881 gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat cgaactgact
    11941 gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa ggatccatcc
    12001 ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc gattttactt
    12061 aatgatgaag tgagatcggc agaccttctg gccattctca aggccctgaa ggccaaaggc
    12121 gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga cggtacggtg
    12181 ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc ggtcattgtc
    12241 ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta cctgatggaa
    12301 gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt taaagcaaca
    12361 atcaaggtcg ctgaccaggg tgaagaaggg attgtggaag ctgacagcgc tgacggtagt
    12421 tttatggatg aactgctaac gctgatggca gcacaccgcg tgtggtcacg cattcctaag
    12481 attgacaaaa ttcctgcctg atgggaggcg gcgcaattgc gccgcctcaa atgatttaca
    12541 tagtgcgctt tgtttatgcc ggatgcggcg tgaacgcctt atccggccta caaaactgtg
    12601 caaattcaat atattgcagg aaacacgtag gcctgataag cgaagcgcat caggcagttt
    12661 tgcgtttgtc agcagtctca agcggcgcag ttacgccgcc tttgtaggaa ttaatcgccg
    12721 gatgcaaggt tcacgccgca tctggcaaac atcctcactt acacatcccg ataactcccc
    12781 aaccgataac cacgctgagc gatagcacct ttcaacgacg ctgatgtcaa cacatccagc
    12841 tccgttaagc gtgggaaaca gtaagcactc tgacggatag tattatcgat aaacgccgga
    12901 tgacacatca cctccagcga acgatcaccc cgatggcttg cgtcatccag cacttgcagg
    12961 aacagtgcct cgctaatctc ttcaccataa aacgcgctac tgaatccctg agaactgcgc
    13021 agattagccg gtaaatcacc agagttagat agcggctgac ggtcgatgcg caacgcaatc
    13081 ccctcttcag ccgcaaacct ggcaacaatc gggaaaatct gcgggaacat atgcacatga
    13141 tgatggctat caagatgcgt aggtttgcgt ccaaatagct caatgaaacg caaatactga
    13201 ctggcaagct cctgagtaat ttcttccagc ggtaaagcat cttcttctgc caactgccag
    13261 atccattttc ccagcacacc atcgcgggtt aaccccggca tagctgtcag tggcttgccc
    13321 atagtaagga caaagtacat ccctatggcc agactcggtt catcacgact caactgcacc
    13381 gcatggtcaa tagcctgccc attcaccagc gccgtcgtcg atgtgacaat cccattgcga
    13441 caggcctcga taatgccgta gttctgacct ttgcttaagc caaaatcatc ggcattaaca
    13501 atcagtaagc gttccataat cagcctcggt taatgtgctt ttttaagctc tgcgatgcag
    13561 tcggcaaaat ttggcagcca tttctcgtgc gccagaatca tctcgcgtgc cagcagctca
    13621 gcatcgcgat cggaatgcac caacggacta aggtttagcg ccagtaacac atcgttaaat
    13681 tctccgctaa gtgcggcgtt gctggcagca atctcgaagc ctttaatggt gtgaatcagc
    13741 cccatcactt tatcatcgaa atgcgtaatg cgcggatgtg gcgtcgcgcc atcgcgcccc
    13801 agcttacagg tcatttctac cgcccagtct gccggaatat tatcaatatg cccatgatgc
    13861 gggatattaa cgtaatgttc agcttgcttg tcgttgtaga tagcgttgat cacttcgcac
    13921 gctgcatcag agtaataagc cccaccgcgc tgttccagtt ctttcggctt aactttcaac
    13981 tccgggtttt tatacagctc aaaaagttgt ttctcgactt tctgtactac ctgcgctcgt
    14041 gcgccgcctt tgtagtattc gcccatttca atagccagca tctctttttg cttgaagtaa
    14101 taaagcaaat aagaacacgg caacagattc agagaacgaa ttaagccttc gctaaatggc
    14161 agatcgaaaa tatttttaac gccagatgct tttaactgcc cggatgccac accatcaagc
    14221 aattcggcaa agcgcgactt gccatttacc agcacatcct taatgaacac catatggttg
    14281 aggccgaaca aatcgataga taaatcatcg ctgtctttca gcatcagaac atcgtgaata
    14341 aacatcttca tgccgatcgg aatattacac acgccgataa agcgtttaaa tccggtatga
    14401 cgataaacgg cttcagtaac cattcccgcc gggttagtga agttaatcac ccatgcattc
    14461 ggacaaagtt cttcgacatc ttttacgatg tcaaaaatca ccggaatggt acgcagacct
    14521 ttaaacagac cgcccgcgcc gttggtttcc tggccaagat aaccatgact taatggaata
    14581 cgttcatcca gttcgcgcgc cggtaattgg cctacgcgca gttgggtagt aacgaaatca
    14641 gcatctttca atgcttcgcg gcgatccagc gttttataaa gcttcatcgg gacgccagcg
    14701 ttatcaatca tccgttggca gagttcaaaa atgatatcca gtttctcttt accatcttcg
    14761 acatccacca gccataattc gctgaccggc aattcgtgat aacgcttaat aaatccttcc
    14821 agtaactccg gggtatagct gctcccgcca ccaatagtga cgacttttaa tttctggctc
    14881 ataatttctc ccttcagtac agaatactga tatctggcat atctgccccc ccggacataa
    14941 ataatccagc aacaggacag atatgtgaat tgtcaggtat aacgacttac tgattaaatt
    15001 cagtcaattt cttacgatag ctcttaggcg taaaggacgt taatttctta aacgttttaa
    15061 taaacaaact cgggctacta taaccggcct caaacgcaat atccgtcacg gaatagttgg
    15121 tcatttccag ttgttttttg gcaaaattaa tacggatctc attaataatc tgcattggtg
    15181 ttttgccata atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg gctgacaacg
    15241 tcaccatatt ctccagcgcc gattcactaa actgctcttt atcatgcatc ttttctaccg
    15301 tacttttcag ccactgcggt acatcatcaa tcacctgttc ttcgcggtaa tgacgtaaac
    15361 ggttaatgac ataaaaagta accatctcaa caaactcttc cagccctgtt tcgcggaaat
    15421 tcaatgaaga aatcactgtt tccacatagg tgagaaaagc gttattggtc cggtatacct
    15481 gcgaagcgac aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa aagcgtttac
    15541 tgatcccaac gttcaatata cgcgtggcac caaactcata aaaactttga tggtgcgaac
    15601 ctaacggaat aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg ttaatctcct
    15661 ggaaataacg cccggttaat accagagtaa attcataata gtcgtgctga tgcagcccgc
    15721 tgatactctc agttttgtta tagataaaca catggaagtt tttgccatta aacaactgct
    15781 gttctcgggc agtggcaatt tccggcgcgt taatcactgg ctgcatcatc gactccttat
    15841 gccttcagtt tttcatgaag ctcaattaat tcagtaatca gttcacgcgc aagcatggac
    15901 gtcattaaat gatcctgagc gtggaccagc accagactca ctttcatctt accttcgccc
    15961 gcatcgcctt caatcagttt cgtctgtacc agatgcgctt cattcaatgc catccgtgac
    16021 tgatccatca tggcttttgc tgcagcaaaa tcgccctgct tcgcctgttt cagtgccgca
    16081 tacgccaggc tgcgcgcttg tccggagttg atgataagcc ccatcaccac ttcttccagc
    16141 tcttcagctt ccgtttgcgt atcgggaatg ttatcgagat ccatcataca tcgttcctct
    16201 ctattcttac cggcacgatt acccgtaccg gcatcgatta aaatttcagg gcgttagcga
    16261 tatcttcttc gcgctcttct ttatcaatcg cgttttgtgc tttgttagcc accacaacaa
    16321 agggcagata aattaacgtt gcgataccaa ggttgaagag tgcaaccagc aatgcggcga
    16381 cgctaccgtt ggtgttaaag aaggctccca gaccggttgg catggtccac ggtgcaatat
    16441 tggtcaccgg aggaatgatg cccatgtagt acgctgcgag ggtgattgcc gccagaatcg
    16501 gttgtaccag tacaaacggg ataaacatca ccgggttcat gataattggc agaccaaaca
    16561 gaatcggttc gttaatctgg aagatgccgg acggcagcgc cagttttgcc acctgacgat
    16621 aatcagcacg gcgagaggcg ataaagatag ccaggatcag gcctaaagtc gcaccactgc
    16681 ccccaaggaa aataaaggag tccagcatcg gcttggccca gatatggaag gtcttaccgg
    16741 ctgccagcgc cgcttcgacg gaaccatatt gctgataggt cgcgatattt tccagcgccc
    16801 acggcgtcat aatgccgttg tccagtgcgg tcagcgccag cgcgccatga ataccgaaga
    16861 accagagcag tggaacaaag atcacatagg cccagcccac cacgctaccc aacgatgcca
    16921 gtggggttga gatggtatcc ataatgatct gatggaagtt ggtgccccag gtattcaacg
    16981 cccaggcaat aatccccatc acggaaagaa taataaagcc gggaattaat gccgagaagg
    17041 aacgcgatac tgaagcaggt acgctatcgg gcaatttaat gacccaattg cggcggacaa
    17101 taaaggtaaa catttctgcc accaccaggc caataataat cccggagatg atattcgctc
    17161 cacctaacca gtttgcgcca accacatagg cctcaccgac actatatggg gtgacggtca
    17221 taaatgctgc aacggataac aacccagccg ccagcgcatc gactttacgc tcttctgcca
    17281 gcgccatgcc aataaagaac ggtgccatta aagacattat ccctaatgtt ccgttatata
    17341 cgttgccgcc aatacctttc aggccattaa gtgtttcaat ggttgaggcg tcgaggcgaa
    17401 tacctaagga ataaaaaaac gccccctccc caaagcttag aaaaacgttg ttaattaata
    17461 caaacatggc ccccgcaagg gttaacggca ttaagcgaat aaagccattt ttgattgcat
    17521 taacgtgtgg ctgctttcct attttaactg caaaagggag gagtaccttt tcaagcgatg
    17581 caataacatt actcatagaa aaataccctt aaaaaccgca atttaaatat tgcggtattg
    17641 atttatgaaa taactctttg acgggaaaat ttaaaataaa ttaatttgct gcggcttttt
    17701 taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca taaagcagcg
    17761 agtcaattac ttcaaccggt ttgttgggta acaaacgctg gatttcgggc aacatataag
    17821 caatctgcgg ccctaataac acgacatcgg cattctgacc tttttcacca gccagtgttt
    17881 ccggaaatgc ttcaataatg accggaactt catatttttc tgcctgtgcg cgcatttttg
    17941 ataccagtaa agaggtagac atgcccgcag aacaaaacag ataaatgtgt ttcttttcca
    18001 taaaactgcc ctcatcgacg attatctgtc agccagacac tccgcaagcc ttaacctgct
    18061 tccatgctct gggtaacttg cgaaaccaac atgacttttt ttgtggctga aaggagtata
    18121 cggtatagac ctgagcaata gtatttcttt gaccttataa attgtttctc cttgacctgt
    18181 ggttatgacc ctcttcacat ttcgggcaaa taattcgcga agataattaa gaactcaggc
    18241 caaaaaaaac cggcgcaatg gccggtttcc gttgttactc aagctttcag acgaattgat
    18301 tacttcgcag cctgtggatc agtgtcgtat tcagcacagg tctgataacc ggagttgatg
    18361 acatgtccgg tatcatctaa cgcgacaaag taggtttctg ctttaccatc acgttgaccc
    18421 aggatgtagg tctggcaagt accacgagca tggatcatgc tcacttcaga cgaaggttta
    18481 cccgcaattt gtgcaacctg cgcccggctc atgccttttt tgacgtcttt caccacaggc
    18541 tgtacaaact ggtctttagt acgatcataa gccgtacaac ccgccagcat ggttaatacc
    18601 gccgctgcac tcagaattcc tgccatattc ttgttcatat tccgtcctct tgtttatcag
    18661 cgtgttagat aagcctggaa tacattgggc gctttttcaa gcccgcgaac gaaacggctc
    18721 cgctttcaga ggattcctgt atgacgtttt aaccaccatt cagcccgctg tcgcttgtcg
    18781 tttcagtggc aacgggttag ctttaaggaa gttttgtctt ttctgtctgg aggggttcaa
    18841 tgacattgca acaacaaata ataaaggcgc tgggcgcaaa accgcagatt aatgctgaag
    18901 aggaaattcg tcgtagtatc gattttctga aaagctacct gcaaacttat ccgttcatta
    18961 aatcactggt gctcgggatc agcggcggtc aggactccac gcttgccgga aagctgtgcc
    19021 agatggcgat taatgagctg cgcctggaaa ccggcaacga atcactgcaa tttattgccg
    19081 tacgcctgcc ctatggtgtt caggccgacg aacaagattg ccaggatgcc attgccttta
    19141 ttcaaccgga tcgcgtatta accgttaata tcaagggcgc ggtattggcc agcgaacagg
    19201 cattgcggga agcaggcatt gaactgagcg attttgtccg tggcaatgaa aaagcgcgtg
    19261 agcggatgaa agcacaatat agcattgcgg gtatgaccag cggtgtcgtg gtgggcaccg
    19321 atcatgcagc agaagccatt accggattct tcactaaata tggtgacggc ggtacagaca
    19381 ttaatccgct gtatcgtctc aacaaacgtc agggtaaaca gttactggcg gcattaggtt
    19441 gcccggaaca cctttataag aaagcgccaa cggccgatct ggaagatgat cgcccttctc
    19501 tgccagatga agtggcactc ggcgtgacct atgacaatat cgacgactat ctggaaggga
    19561 aaaacgtacc tgaacaggtc gccagaacaa tagagaactg gtatctgaaa accgaacata
    19621 aacgccgtcc gccaattacc gttttcgatg atttctggaa aaagtaataa tttgcacata
    19681 atggtttgtg cgaaaaagag taatttgttc acgccggatg cggcgtgaac gccttattcg
    19741 acctacaaaa ctatgcaaat tcaatatatt gcaggagtcg aataggcctg acaggcgtag
    19801 cacgtcagac ggtgtaacct ttgtcatcga cccgcctctt ttttaatcgc tccccgcctg
    19861 ttacactgga tagataacca gcattcggag tcaacagtgg tacggcgttt aacttctccg
    19921 cggctcgaat ttgaagctgc ggcaatttat gaatatcccg aacatttacg ttcattcctt
    19981 aatgacttac ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga caccatgccg
    20041 ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc tttctcattt acgcaccccg
    20101 gatgaagccg ccatgctacg gcaatcccgg cgaattagct ggatctgtac cgcaggcgaa
    20161 atcggcgctc tgctccttga agcgcgatta atcaaagaac aacagccgct gtttaataaa
    20221 cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg cgtcgatgtg
    20281 gtgtatgcca aagaggtgga tttttcacga gcccccaacc tgtttggcct gtttgccaat
    20341 aggcgcgcag ctttgcaagc attgcagacc atcgctgatg aacaaaaact ttgttatggc
    20401 ctgctgggac tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc gctaaaacgt
    20461 tgcgccggag catgctgcgg taaagagagc catgaggaac atgcgctacg cttgcgccag
    20521 tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag gtgcggtggc actgaaagaa
    20581 cagcacccgg aaatgactca atatcatatt attcaaaact ggctgtggct gggggcggtt
    20641 aattcgctgg aagaagcgac aacgttaatt cggacacccg ccgggtttga tcacgacggt
    20701 tataaaattc tttgtaagcc gctgctttcc ggtaactatg aaattactga acttgatccg
    20761 gcgaatgacc agcgagccag ttgatttcac tgaacaacag tttaccttgc ggttgtaaaa
    20821 gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg agcaatttat
    20881 cgcccagttg ccacgccccg ttaatcacaa acaccacccc gccgcgtgag ccaaaggtag
    20941 taaacgtacg ttcggcaatt ctgactttcg ccttacagac atccaggcgt gtcatgatgt
    21001 tgaaatccat cgacatctgc ccttccgtca gtttcgcttt taccacctgg tccgctgcaa
    21061 aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg gtctgcgctt tcaaggagca
    21121 tctcgccgcc ttccagcaac gtcactatcc tttccatgcc gggaaacaaa gaaaactccc
    21181 cattcgccgc gatggatgca atgctggcac gccagtaaaa atcacgtttt gccggtggga
    21241 aagtgcaaat ttcgcgcgtt tcaccggcag catttcgcca cagattcacc gacattttac
    21301 gcatatcaaa gtattccata ctcgctcccg gcccgttctt ttcattgtta tcgttcaggt
    21361 aaccgtggat ggt
//
LOCUS       DAJTYZ010000070        21239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000070 DAJTYZ010000000
VERSION     DAJTYZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.070"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(179..724)
                     /gene="hemG"
                     /locus_tag="OO596_002448"
     CDS             complement(179..724)
                     /gene="hemG"
                     /locus_tag="OO596_002448"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6718285.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(736..2187)
                     /gene="trkH"
                     /locus_tag="OO596_002449"
     CDS             complement(736..2187)
                     /gene="trkH"
                     /locus_tag="OO596_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6718286.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2226..2840)
                     /locus_tag="OO596_002450"
     CDS             complement(2226..2840)
                     /locus_tag="OO596_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6718287.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2840..4171)
                     /gene="pepQ"
                     /locus_tag="OO596_002451"
     CDS             complement(2840..4171)
                     /gene="pepQ"
                     /locus_tag="OO596_002451"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6718288.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVELLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4361..6550
                     /gene="fadB"
                     /locus_tag="OO596_002452"
     CDS             4361..6550
                     /gene="fadB"
                     /locus_tag="OO596_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6718289.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6560..7723
                     /gene="fadA"
                     /locus_tag="OO596_002453"
     CDS             6560..7723
                     /gene="fadA"
                     /locus_tag="OO596_002453"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6718290.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7913..8614)
                     /gene="fre"
                     /locus_tag="OO596_002454"
     CDS             complement(7913..8614)
                     /gene="fre"
                     /locus_tag="OO596_002454"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6718291.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8661..10154)
                     /gene="ubiD"
                     /locus_tag="OO596_002455"
     CDS             complement(8661..10154)
                     /gene="ubiD"
                     /locus_tag="OO596_002455"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6718292.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10321..10809
                     /gene="rfaH"
                     /locus_tag="OO596_002456"
     CDS             10321..10809
                     /gene="rfaH"
                     /locus_tag="OO596_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6718293.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10806..11588)
                     /gene="tatD"
                     /locus_tag="OO596_002457"
     CDS             complement(10806..11588)
                     /gene="tatD"
                     /locus_tag="OO596_002457"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6718294.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11630..12406)
                     /gene="tatC"
                     /locus_tag="OO596_002458"
     CDS             complement(11630..12406)
                     /gene="tatC"
                     /locus_tag="OO596_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6718295.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12409..12924)
                     /gene="tatB"
                     /locus_tag="OO596_002459"
     CDS             complement(12409..12924)
                     /gene="tatB"
                     /locus_tag="OO596_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6718296.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12928..13197)
                     /gene="tatA"
                     /locus_tag="OO596_002460"
     CDS             complement(12928..13197)
                     /gene="tatA"
                     /locus_tag="OO596_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6718297.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13276..14916)
                     /gene="ubiB"
                     /locus_tag="OO596_002461"
     CDS             complement(13276..14916)
                     /gene="ubiB"
                     /locus_tag="OO596_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6718298.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14913..15518)
                     /gene="ubiJ"
                     /locus_tag="OO596_002462"
     CDS             complement(14913..15518)
                     /gene="ubiJ"
                     /locus_tag="OO596_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6718299.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15532..16287)
                     /gene="ubiE"
                     /locus_tag="OO596_002463"
     CDS             complement(15532..16287)
                     /gene="ubiE"
                     /locus_tag="OO596_002463"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6718300.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16382..17809)
                     /gene="rmuC"
                     /locus_tag="OO596_002464"
     CDS             complement(16382..17809)
                     /gene="rmuC"
                     /locus_tag="OO596_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6718301.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17950..18711)
                     /gene="udp"
                     /locus_tag="OO596_002465"
     CDS             complement(17950..18711)
                     /gene="udp"
                     /locus_tag="OO596_002465"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6718302.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18973..19785
                     /locus_tag="OO596_002466"
     CDS             18973..19785
                     /locus_tag="OO596_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6718303.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20179..21239
                     /gene="hcp"
                     /locus_tag="OO596_002467"
                     /pseudo
     CDS             20179..21239
                     /gene="hcp"
                     /locus_tag="OO596_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5219 a         5682 c         5334 g         5004 t
ORIGIN      
        1 acagagtcaa gcatttattt ttgctttctc tgtcggaatt ctcaggagaa ccccgctgac
       61 ccggcggcgt gtttgccgtt gttccgtgtc agtggtggcg cattataggg agttattccg
      121 gcctgacaag aggaaattta aaataatttt ctgaccgcgc attttttatt ctttatgctt
      181 atttcagcgt cggtttgttg gttaactggg cgatttctcg ggcgaaattc gccacctgct
      241 cccaatcggt atagacaact tctttgcgcg tatccgtttc accgcctgac atcttcataa
      301 ttagcttgat cataaaacga tcgtaccagc gataacgtgg gtaacgcagc gccccggcaa
      361 tgaccgcgca gcgatcggga cgccattgcg agttcatcag aaacttccgc gcgtagctgt
      421 tggtctgtgg agtacgcttc tccggtttgc gcgccaccag gtttacggaa taaaaggcgc
      481 tcggcatcga attcagtcgc gtcgcatgtt ttttgacaaa ttcctggaac gcagagtggt
      541 agtgaccata gcgaatagaa gcaccaatga ccacacggtc ataattttcc cattgtggtt
      601 cttcaatgcg gtgcacattg gcgacatccg tctggatccc cagttctttc agttccgaag
      661 ccaggtagga ggcaatctcg cgcgtttgtc cgtcccttgt tgagaaaaga attaatgttt
      721 tcacgtatta ctccatcatt cacgccagaa agtcggggta aagagcacca gcaatgtaaa
      781 gacctcgaga cgaccaaaca gcatgttggc aatcaggatc catttagcca ccgggttcat
      841 actggtaaag ttatcagcaa ccacgccaag ccctggcccc aggttattca atgtcgcaac
      901 aactgaagca aaggcagaaa agtcatccac gcccgtggcg ataatcgcca gcatactgac
      961 aataaacacc aatgcatagg cggagaaaaa tccccaaacg gcttcgagga tacgttccgg
     1021 cagtgcgcga ttccccagct taatgctata gacggcgttc ggatgcacca gtcgtttcag
     1081 ctcacggttc ccctgcttaa acagcagcag gatgcggatc actttcaggc caccgcccgt
     1141 tgacccggcg caaccgccga taaaagctga acataaaagc agtaccggca aaaagagcgg
     1201 ccagcgggca atgctgtcag tcgtaaaccc ggcggttgtc gccatcgata ccacctggaa
     1261 aaacgcctgg ttaattgtca tcagcgccga actgtagaca ttatgaaacc acagtacgag
     1321 tgtacaaata accaccagcg taaactgtac gccgataaac atgcgaaatt ccggatcgcg
     1381 ccaataaacc ttcagactac gcccacttaa cagtgaaaag tgcagaccgt agttacagcc
     1441 ggagatcagc aggaagatag caatgatagt gttaatagtc gggctgtcga aataaccgat
     1501 actggcatca tgtgtcgaga agccgccaat agcgatagtc gcaaagctat ggccgatggc
     1561 atcaaaggca tccattccgg caaaccacaa cgccagcgca caggcgacgg tcagcaagac
     1621 ataaatcaac cacagggttt tcgccgtttc cgcaattcgc gggcgcattt tgttatcttt
     1681 cagcgggccg ggcatttctg cgcgatagag ctgcatccca cccacgccga ggataggcag
     1741 tatcgcaacc gctaacacga tgatccccat cccgccaaac cattgcagca tctggcgata
     1801 aaagaggatg gcatgaggga gcgaatccag ccccaccagc gtagtggccc cggtggtggt
     1861 caggccagag aaagattcaa aaaacgcatc ggtaatcgtg aggttcgggc tttccgagaa
     1921 gataaaaggg agcgcaccga cactacccag cacggtccag aacagcacca ctatcagaaa
     1981 cccctcacgg gatttcagtt cgcccttctc tttacggttc ggccaccaca gcatagaacc
     2041 aatggcgagg gcgacaaaaa aggtctgggt aaaagcgcgg cccgctccat cccggtagat
     2101 gagtgctacc agcccaggga taatcatggt ccctgaaaat aagatgacca gtagtccaac
     2161 gattcgggta atggcgcgaa aatgcatctc tgccgcttcc ttagttctgc aaaacgaagt
     2221 ggggattatt cttcaatcgc taacaattgc aatgaaccac ggctaaaatc cgccagcttt
     2281 gcggaaaatt cagccacttt cgccgccgga agcgccaccc gcagcagaac gaatgcctga
     2341 taatcactgt tgataatttt gccgtcacac tgccccagca acgcttcaat gccggttaac
     2401 tgatgatatt cacattgcaa agtatattcg gttaatggcg tcttgcgttg ggtcgttagc
     2461 tggcgcagcg cctgattcac gccgccgcca tacgctttca ctaacccacc ggtgccaagc
     2521 aatatgccgc cgtagtagcg cactaccaca gaggtaattt ccccgacgcc gctgcccatt
     2581 agctgggcga gcatcggttt acctgccgtt cctgccggct ccccgtcgtc agagaaaccc
     2641 agctgttgag aatcatccgg cgcacccgcc acccacgcca cgcaatggtg gcgggcatcg
     2701 gggtgttctg cccgcaccga ttcaacaaac gctttcgccg cctcaacgcc atcggtatgc
     2761 gccaacatcg ttatgaagcg gctttttttg atctcttcaa cgaccgtgac cggtgccgca
     2821 ggaattaacc agctttccat cacgccagtt tcagatcccg ggtcatgttt tccacgttgt
     2881 tttcgtggat caccacgttg tcttcgatac gaatgccgcc gaacggtttc agtgcttcaa
     2941 ttttctgcca gttgaagtgc ttgctgaact gcccttcacg ccacggtgcc agtagcgatt
     3001 caatgaagta gatacccggt tcgatggtta acaccatacc cggctggaga atacgggtgc
     3061 agcgcaggta cggatatttt gccggtgccg cgaggtgtgt accgctatca tcctgcataa
     3121 aaccggctac gtcatgcacc tgcaggccca gcggatgacc gataccatgc ggcataaacg
     3181 gtccggtgag atcgttttcg accatcgctt cttcactcat atcggtgatg atttgatgtt
     3241 tacgcagcag tttggcgatg cgctgatgga actggatgtg gtaatccaca tagctaacgc
     3301 ctgctttcat ggtggcgatc agcgccagtt gttcatcatt tacgtctttc accagctgtg
     3361 cgtagtcgtt gtcgcttttt gccgaccagg tacgagtcag gtcagccgca tagccgttat
     3421 attcggcccc ggcatccagc aggaagctgc gcatctcttc cggtgcctga tgatccagtt
     3481 tggtgtaatg cagcaccgaa gcgtgttcgt taagcgccac aatgttgctg taaggtacgt
     3541 cggtatcacg atgaccggtc gcggtcagat aggcgatgtt gatatcgaac tcgctcatgc
     3601 cagaacggaa cgcttcttct gccgcacgat gaccgttgac cgccattttc tgcgcttcac
     3661 gcatacaggc cagttcgtac tcggttttga aggagcggta gtaatgcagg tagtcgatca
     3721 cccctttcgg gttgatgttg ctggcctcaa tacccagttg cagcgcacgt tccggcaccg
     3781 gaccgatata accgatattg ccgcgcgcag cgggcagcag gctaccaatg ccatcggctt
     3841 tcggcagtgc gatcacttcc acatcttcag tccagaagga ggtcggcagc agttcgacgt
     3901 tgtgccagta atcaaccggc agatagaacc acagtttcgg cttgttcacg ccatccacca
     3961 gcaaccagca gtttggcacc tgagttaccg gcacccacgc tttgaattgc gggttcactt
     4021 taaacggata gggatgatcg tcgagaaaaa cgttgaacag ctcgccggag tgaataagta
     4081 atgcatccag cttgaagcgc gccagcgcat cgcgagtccg ttcttgtaag gtagctatat
     4141 gatttttata gagcgaggcc agtgattcca ttttttaccc ttctgttttt ttgaccttaa
     4201 gtctccgcat cttagcacat cgttcatcca gagcgtgatt tctgccgagc gtgatcagat
     4261 cggcatttct ttaatctttt atttgcatat ttttaacaca aaatacacac ttcgatgcat
     4321 ctggtacgac cagatcacct tgcggattca ggagactgac atgctttaca aaggcgacac
     4381 cctgtacctt gactggctgg aagatggcat tgccgaactg gtatttgatg ccccaggttc
     4441 agttaataaa ctcgacactg cgaccgtcgc cagcctcggc gaggccatcg gcgtgctgga
     4501 acagcaatca gatctaaaag ggctgctgct gcgttcgaac aaagcagcct ttatcgtcgg
     4561 tgctgatatc accgaatttt tgtccctgtt cctcgttcct gaagaacagt taagtcagtg
     4621 gctgcacttt gccaatagcg tgtttaatcg cctggaagat ctgccggtgc cgaccattgc
     4681 tgccgtcaac ggctatgcgc tgggtggtgg ctgcgaatgc gtgctggcga ccgattatcg
     4741 tctggcgacg ccggatctgc gcatcggtct gccggaaacc aaactgggca tcatgccggg
     4801 ctttggcggt tctgtacgta tgccacgtat gctgggcgct gacagtgcgc tggaaatcat
     4861 tgccgccggt aaagatgtcg gcgcggatca ggcgctgaaa atcggtctgg tggatggggt
     4921 agtcaaagca gaaaaactgg ttgaaggcgc aaaggcggtt ttacgccagg ccattaacgg
     4981 cgacctcgac tggaaagcga aacgccagcc gaagctggaa ccgctaaaac tgagcaagat
     5041 tgaagccacc atgagcttca ccatcgctaa aggaatggtc gcgcaaacgg cggggaaaca
     5101 ttatccggcc cccatcaccg cagtaaaaac cattgaagct gcggcccgtt ttggtcgtga
     5161 agaagcctta aatctggaaa acaaaagttt tgtcccgctg gcgcatacca acgaagctcg
     5221 cgcactggtc ggcattttcc ttaacgatca atatgtaaaa ggcaaagcga agaaactcac
     5281 caaagacgtt gaaaccccga aacaggctgc tgtgctgggc gcaggcatca tgggtggcgg
     5341 tatcgcttac cagtccgcgt ggaaaggcgt gccggttgtc atgaaagata tcaacgacaa
     5401 gtcgttaacc ctcggcatga ccgaagccgc gaaactgctg aacaagcagc ttgagcgcgg
     5461 caagatcgat ggtctgaaac tggctggcgt gatctccacg atccacccaa cgctcgacta
     5521 cgccggattt gaccgcgtgg atattgtggt agaagcggtt gttgaaaacc cgaaagtgaa
     5581 aaaagccgta ctggcagaaa ccgaacaaaa agtacgccag gataccgtgc tggcgtctaa
     5641 cacttcaacc attcctatca gcgaactggc caacgcgctg gaacgcccgg aaaacttctg
     5701 cgggatgcac ttctttaacc cggtccaccg aatgccgttg gtagaaatta ttcgcggcga
     5761 gaaaagctcc gacgaaacca tcgcgaaagt tgtcgcctgg gcgagcaaga tgggcaagac
     5821 gccgattgtg gttaacgact gccccggctt ctttgttaac cgcgtgctgt tcccgtattt
     5881 cgccggtttc agccagctgc tgcgcgacgg cgcggatttc cgcaagatcg acaaagtgat
     5941 ggaaaaacag tttggctggc cgatgggccc ggcatatctg ctggacgttg tgggcattga
     6001 taccgcgcat cacgctcagg ctgtcatggc agcaggcttc ccgcagcgga tgcaaaaaga
     6061 ttaccgcgat gccatcgacg cgctgtttga tgccaaccgc tttggtcaga agaacggcct
     6121 cggtttctgg cgttataaag aagacagcaa aggtaagccg aagaaagaag aagacgccgc
     6181 cgttgaagac ctgctggcag aagtgagcca gccgaagcgc gatttcagcg aagaagagat
     6241 tatcgcccgc atgatgatcc cgatggtcaa cgaagtggtg cgctgtctgg aagagggcat
     6301 tatcgccact ccggcggaag cggatatggc gctggtctac ggcctgggct tccctccgtt
     6361 ccacggcggc gcgttccgct ggctggacac cctcggtagc gcaaaatacc tcgatatggc
     6421 acagcaatat cagcacctcg gcccgctgta tgaagtgccg gaaggtctgc gtaataaagc
     6481 gcgtcataac gaaccgtact atcctccggt tgagccagcc cgtccggttg gcgacctgaa
     6541 aacggcttaa ggagtcacaa tggaacaggt tgtcattgtc gatgcaattc gcaccccgat
     6601 gggccgttcg aagggcggtg cttttcgtaa cgtgcgtgca gaagatctct ccgctcattt
     6661 aatgcgtagc ctgctggcgc gtaacccggc gctggaagcg gcggccctcg acgatattta
     6721 ctggggttgt gtgcagcaga cgctggagca gggttttaat atcgcccgta acgcggcgct
     6781 gctggcagaa gtgccgcact ctgtcccggc ggttaccgtc aatcgcttgt gtggttcatc
     6841 catgcaggca ctgcatgacg cagcacgaat gatcatgact ggcgatgcgc aggcaggtct
     6901 ggttggcggc gtggagcata tgggccatgt gccgatgagt cacggcgtcg attttcaccc
     6961 cggcctgagc cgcaatgtcg ccaaagcggc gggcatgatg ggcttaacgg cagaaatgct
     7021 ggcgcgtatg cacggtatca gccgtgaaat gcaggatgcc tttgccgcgc ggtcacacgc
     7081 ccgcgcctgg gccgccacgc agtcgggcgc atttaaaaat gaaatcatcc cgaccggtgg
     7141 tcacgatgcc gacggcgtcc tgaagcagtt taattacgac gaagtgattc gcccggaaac
     7201 caccgtggaa gccctcgcca cgctgcgtcc ggcgtttgat ccagtaagcg gtacggtaac
     7261 ggcgggtaca tcttctgcac tttctgatgg tgccgcggcc atgctggtga tgagtgaaag
     7321 ccgcgcccgt gaattaggtc ttaagccgcg cgcccgtgtg cgttcgatgg cggtcgttgg
     7381 ttgtgatccg tcgattatgg gttacggccc ggttcctgcc tcgaagctgg cgctgaaaaa
     7441 agcggggctt tctgccagcg atatcggcgt gtttgagatg aacgaagcct ttgccgcgca
     7501 gatcctgccg tgcattaaag atctcgggct aatggagcag attgacgaga agatcaacct
     7561 caatggtggt gcgatcgcgc tgggtcatcc gctgggttgt tccggtgcgc gtatcagcac
     7621 cacgttgctg aatctgatgg aacacaaaga cgttcagttt ggtctggcga cgatgtgtat
     7681 cggtctgggt cagggtattg cgacggtgtt tgagcgggtt taaccacttg ccggatgcgg
     7741 cgtgaacgcc ttatccggtc taccgatcct gcaccaatgt aggcctgata agacgcgcca
     7801 gcgtcgcatc aggcatcgtg caccgattgc cggatgcgac atagatctaa atgccactga
     7861 ataaagtttt agttgccgtt cttcccgcct gtcaggggcg ggtttttata tctcagataa
     7921 atgcaaacgc atcgccaaac agacgatctt cccgcgcatt acgctcactg caaaacaggt
     7981 cacgagcaat tttcgccatc tcaaaacgtc cggcaatata gatatcatgc tctgccagcg
     8041 taccatgatc ctgcaagacc gccgttaaca cggtgccagt acgcccacgc cagcccgctt
     8101 ccggttgttc aaccaccggc accacttgca gaccaggatg cttcagcgaa agcgcctcaa
     8161 gctcgcagag atcatacaga tgttgctctt cacgcccacc ccagtaaatg gtgatatcgc
     8221 gattgggatt acgcgccaac gccgtcaata aaatcgagcg cgcataggag aacccggtgc
     8281 cgcccgcaat caaaatcatc ggacgctctt catcatcgcg cagccacgct tctccgtggg
     8341 gaatatcgac cacgatttga tgatctttga ggatgcggtc catgactgct ttcgcgtaaa
     8401 ggttgatttc agaagcgcca atatgcagct cgataaaccc tttttcatcc ggcgtcgaag
     8461 ccattgaaaa cggacgtttg tcgcgctcat ccatcactac catcaaatac tgaccagcac
     8521 gaaaagaaaa ggccgcgtct ggtacgattc ggacacggta tacagtatcc gtgatagctt
     8581 ctaccgaggt cactttacag cttaaggttg tcatgcgctt tctctgtcgg atcaaataaa
     8641 tagggcaaaa ctaacgcgca tcaggcgctt ttaccgttgt taaaaatagc cagttcatcc
     8701 cagatggcgt caatatgcgc gacgacatca ggatcttttt tgatgggacg tccccattca
     8761 cgctgggttt cccccggcca tttattcgtg gcatccagcc ccatttttga acccagcccg
     8821 gagacaggcg aggcaaaatc cagataatca ataggcgtat tttctaccag aacagtatcc
     8881 cgcgccgggt ccatacgggt ggtaatcgcc caaatcacat cgttccagtc gcgtgcgttg
     8941 acgtcatcat cgcaaacgat cacaaattta gtgtacataa actggcgtaa gaacgaccag
     9001 acgcccatca tgacgcgctt cgcgtgtccg gcgtactgtt ttttgattgt cactaccgcc
     9061 agacgataag aacagccttc cggtggcagg taaaaatcga caatttccgg gaactgtttt
     9121 tgcagaatcg gcacaaacac ttcgttcagt gcgacaccca gcaccgcggg ttcatccggc
     9181 ggacgaccag tataggtgga gtggtaaatc gcatcttcac gctgggtaat atgcgtcacg
     9241 gtaaataccg ggaaactatc gacttcgtta tagtaaccgg tgtggtcgcc atacggccct
     9301 tccggcgcag tttcgccttg ttcgatatac ccttccagca caatctccgc actggcgggc
     9361 acttcaaggt cattggagat acacttcacc acttcggtct tggtaccgcg cagcaatccg
     9421 gcaaacgcat actctgaaag tgtatccgga acgggggtga ctgcaccgag aatcgtggcg
     9481 ggatcggcac ccagcgccac agaaaccggg aaacgttcgc ccggatgcgc cgcacaccac
     9541 tcctgataat ccagcgcgcc gccgcgatgc gacagccagc gcataatcag tttgttttta
     9601 ccaatcagct gctggcgata aatgcccaga ttctgccgct ctttatgcgg gccgcgcgtt
     9661 acggtcagcc cccaggtaat cagcggcgcg gcatcttccg gccagcaggt cataatggga
     9721 atgcgattga gatcgacgtc atcgccagag acgatttttt gttggcaggg cgcaccacgc
     9781 agccgctttg tcggcatgtt caatacttgc ttaaactgcg gcagtttatc aaacaggtcg
     9841 cggaaacctt ttggcggctc gggctctttc agaaacgcca ataatttacc aacttcacgc
     9901 agcgccgaaa catcttcctg ccccatgccc atcgccacgc gctttggcgt accgaacagg
     9961 ttgcacagca ccggcattga gtagccttta gggttttcga acaacagcgc aggcccaccg
    10021 gcacgcaaag tgcggtcagc aatttcagtg atttccagat gcggatccac cgggagcgtg
    10081 atacgtttta gctcaccctg ctgttcaagc agcgtcaaga agtcgcgtaa atcgttatat
    10141 ttcatggcgt ccattgtagc ctcttaatct acgcccatta tacggcgttc atctttgcga
    10201 tgctgtaaat ttgttaaatt agcgtgaact ctgacggtat aacgcaaacc ggggaatata
    10261 attaacttag cgtaaagctt ttgctatcct tgcgccccga ttaaacggat aagagtcatt
    10321 atgcaatctt ggtatttact gtactgcaag cgcgggcaac ttcaacgtgc ccaggaacac
    10381 cttgagagac aggctgtgaa ttgcctggca ccgatgatca ccctggaaaa aatcgtgcgt
    10441 ggaaaacgta ctgcagtcag tgagccattg tttcccaact acctgtttgt cgaatttgat
    10501 ccagaagtga ttcataccac gactatcaac gcgacccgcg gtgtcagcca cttcgtgcgc
    10561 tttggcgcgt cgctagcgat agtcccatcg gcggtgattc atcagctatc ggtatataaa
    10621 ccgaaagaca ttgtcgatcc ggcaacccct tatccgggag ataaggtgat tattaccgaa
    10681 ggcgcgttcg aaggctttca ggccattttc accgaacccg atggtgaggc tcgctccatg
    10741 ctattgctta atcttattaa taaagagatt aagcacagtg tgaagaacac cgagttccgc
    10801 aaactctaaa acgcaatccc aaaccgtgtt ttgacattag catccgtggt ggcagccagc
    10861 catgcggcat cttctccacg ccagtgcgca atacgttgca aaatatgggg cagatgggct
    10921 ggctcgttgc gccgggatga tggctttggc gtgagatcgc gagggagcag atacggcgca
    10981 tcagtttcga tcagtaattt ttccgccgga atcaacggca aaagttcccg cagctccagt
    11041 ccgcgtcgtt catcgtaaac ccaaccggta atgccgatat aaattccatg cgccacgcac
    11101 gcctgcatct cttcgcgtgt gccggtaaag caatgaagaa ccgcaccagg cagtttatcc
    11161 agccacggct ccagcaatgt cataaaccgc tcgtgggcat cgcgacagtg cataaatacc
    11221 ggcatgttta aatctgcggc aatgcgtagc tgggcaacaa aagcgcgttc ctgctcttcc
    11281 ggcgtcgaaa agttgcggtt aaagtcgaga ccacattcac caatcgccac cacttctggc
    11341 tgcgcggcca gctcaataat cgcttcttca gtcgcagctt gccactggct gctgtcgtga
    11401 ggatgtacgc ccgccgttga ccaacaggac gaatactgac gcgccagctt ttgcgcctgc
    11461 tggctttcac gcaggttagt gccggtgatg agtagcccat taactcccgc gtcaaaagcg
    11521 cgcgctacaa catcatcacg gtctttcgca aattgcgaac tggtcaaatt aacgccgata
    11581 tcaaacatcc tgtactccat atgacaaccg ccctgacggg cggttgaatt tattcttcag
    11641 ttttttcgct ttctgcttca gcgtcgtttt cctcttcccg atttcgccct ttaccaacgt
    11701 aaaagcgtga gaagaagaca ccgatttcaa acagacagta catcgggatc gccaatagcg
    11761 tttgcgagaa gacatccggc ggcgtcagca acatcccgac aacgaatgca ccaaccagca
    11821 catacgggcg ttttttgcgt aagtcttctg gcgaggtaat ccccatccag cacagcagca
    11881 caattgccac cggcacttca aaggagacac caaacgccat aaacagcgcc ataacgaagc
    11941 ttaaatagct ggcgatgtcg gtggatacct gcaccccttc cggcgcggta ttggcaagga
    12001 agccaaatgc cagcggaaag accacaaagt aggcgaatgc catgccgata taaaacagca
    12061 gagagctgga aaccagcagc ggcaccacca ggcgacgttc atgcttatac agcgctgggg
    12121 cgataaatgc ccacacctga tagagaatca ccggcgctga cagaatcagc gacaccataa
    12181 aggtcagctt gatcggcgta aagaacggcg aggccacgtc ggtggcgatc atcgttgaac
    12241 cttgcggcaa ctgcttgatc aatggcgcgg ataccaggtg atagatgtca ttggcgaaat
    12301 agaccagaca caggaatatc acgatcaccg cgataatgca gttcagcaga cgcttacgca
    12361 gctcaattag atgcgtgata agcggttgag tatcttctac agacatgttt acggtttatc
    12421 actcgacgaa ggggaaggtg cagcggtttt cggttcagcg tccgcagcag gttttaccac
    12481 cggctctggc gtggtttctg gcttctgttc cggcgaactg gcctgcgttt gtgcagcggc
    12541 aggcgttacg ccctcatgcg cagcttcatt atctttcacc accgggttat ggatggtgtg
    12601 cgcttcatcg ctcgcctttt caggatcgtt tgcaacgtag gaacgcttca tcgactccgc
    12661 ggcctggcgt agttcatcca tcgacgcttt cagttcgggc gtcaggttag tgaggctcgc
    12721 cttttcaacc tttttcagac tgtcctgaaa ctcctggagt tttaactcct gggtcagttc
    12781 gttctgcacc gttgtcgcca gtgaacgcaa cgcgcgaatc cagcccgcta ccgtttttac
    12841 cgccacaggc agtcgttgcg gccccagaac gacgaggccg atgatgaaca ccaatagcag
    12901 ttcgctaaaa ccgatatcaa acacggatta cacctgctct ttatcgtggc gcttcgcgtc
    12961 ttctgtttta gcctgttcct gattcgtatc cgcctgctta tcggcgatag ttttcgcagt
    13021 aaaatcagca tcctgactgg ttttatcctg ctttggttca tcatcgctca ttgctttttt
    13081 aaagcctttg atcgacgcac caagatcgga accgatggag ccgagctttt tggtgccaaa
    13141 aagcagtaca acgatgacgg caataatcaa taactgccaa atactgatac cacccataca
    13201 tgttcctctg tggtagatga tgattaaaca aggccgcatt atacgttaca cgacccgcct
    13261 tgagcgatga aaaaatcagc gtgttttgcg ccaaccgaca aaccaggcga tcagaccacc
    13321 tgccattaac cagccgggca tcagccccca ttcaggtcgg ctgaccaaca agaatgtgcc
    13381 acttaatact aacgtagcgc caattccgag aaaataacgc gattgtccct gacgtacatg
    13441 atttgactga agctcgcggg caatcttatc aacactgtgc tgtaaatact tgccctggcg
    13501 caaactgtcg taaaccaatt caggcagttc tggcattttt tcgacccaga acggcgcttt
    13561 ttctttaaat gctctcacca gggcaggaat accgacctga tctttaatcc acgactccag
    13621 gaaaggcttc gctgttttcc ataaatcgag ctgcggataa agctggcgtc ctaccccttc
    13681 gacatagagc agggttttct ggagtaacac cagttgcggc tgcacttcca tattgaagcg
    13741 acgcgccgta ttaaacagat ttaacagtac atgtccaaac gaaatttcgg ccagcggttt
    13801 ctcaaagata ggttcacaga ccgtacgaat ggcaaattcg aactcttcaa cgttggtatc
    13861 tggtggcacc cagccagaat cgacgtgtag ctctgccact ttgcgatagt cgcgattaaa
    13921 gaaggcgata aagttttctg ccagatagcg tttatcttct ttgtttagcg agccaacaat
    13981 cccgcaatca atgccgatat atttcgggtt ttccgggtgt tcatagctta cgaaaatgtt
    14041 gccagggtgc atatcggcat ggaaaaagct gtcgcgaaag acctgagtga agaacacctg
    14101 cacgccacgt tccgccagca atttcatgtt ggtgccattt ttctccagcg tcgcaacatc
    14161 agacaccgga atgccgtaaa tgcgctccat caccatcatc ccttcactac aatagtcagg
    14221 gtaaacttcc gggatgtaga gcatcgggct gtcttcaaaa tttcgccgaa gctgaatggc
    14281 gttggcagat tcccgcagca aattcagttc atcaatcaag gtcttttcgt actcgcgcac
    14341 cacttcggtt gggcgcagac ggcgaccatc cggcagcaaa cgcggcaccc agcgagccag
    14401 acggtagata agtttcagat ccgctttaat aaccggcaaa atatccgggc ggatgacttt
    14461 aatcaccacc tctttaccat tcgatttcaa tcgcgcggta tgaacctggg cgatagaagc
    14521 agaagccagt ggcttgattt caaaatcgtc aaaccacgct tctaccggca agccgcccat
    14581 tgcagcttca atctgctgct tcgccagctt gccatcaaac ggagcaactt tgtcctgcaa
    14641 taacgccagc tgatcggcaa tatgcggtgg aaaaagatcg cggcgggttg ataacatttg
    14701 cccgaacttg atccaaaccg gccccagttc ttgcagggcc agtcgtagtc gctcacctaa
    14761 aagtttgtct ttatgccgat ttggcatcca gaataatgag tatcgccata gccgtagcgg
    14821 cagggtgata cgcattttgg ggatcagttc atcaagtccg tagcttaaaa aagtgcgaat
    14881 gatgaaatat aggcgccgta cttcacctgg cgtcatttag cctccagttt ttccagccgt
    14941 ttggtcaggg catcaacagc acgctcgacg gcagccgtct cttccgcaaa ccaggccact
    15001 tcaagcggac cgggtgccat acgccactct tcagtaatgg cttccgccac ataacgttgc
    15061 tggcgcttaa tgccgtgatg caggaacttt gcgcctccgc gcatggcttt gctgattcct
    15121 tcagcggcga tatcaccggt ataaggggcc agcagttccg cagggtcgaa ctctgccaga
    15181 tctgccagcg caacgaagtt ttgcaccacc tgaatatcgc cctgcacttc cagctcacca
    15241 ctgcgaatca gtgcggtaag ctgctggcga tcgcgaagtt tcggcaacac actggcgtag
    15301 gcgataacgg tgcagtcagc atcgcctgcc cattcgccca gtacatcaac ctggcgttcg
    15361 ctgaacacca gaatcaatga cgtcgaaaag ccttttacct ccacgcgcaa tactttaccc
    15421 agcagacgcg agcgggctgt tttcagcgcg ggtgagcgat acaggaaggt gttgagcaga
    15481 ctttcaattc ctgccgtcac taaaggttta aaaggcattt ccggtctcct gtcagaactt
    15541 ataaccacga tgcagcgcca caacccctgc cgtcagattg tagtagtcga cactttcgaa
    15601 tccggcatcc tgcatcatgg ctttcagggt atcctgatcg ggatgcatac ggatggattc
    15661 tgccagataa cggtagctgt cggcgtcgtt cgcgaccagt gagccaatac gcggcagcac
    15721 atggaaggag tatgcatcat aggctttgct cagcggctcg ataattggct tcgagaactc
    15781 aagcaccagc aggcggccgc cgggtttcag cacgcgatac attgaacgca gtgctttatc
    15841 tttgtcggtg acgttacgca gaccaaacga aatggtgatg caatcaaagg tgttatccgg
    15901 gaacggcagc gcctcagcgt tcgcctgaac atactcaacg ttgccaatca caccgatatt
    15961 acgcagcttc tcgcgtccca ttttgagcat ggattcattg atatcagcaa ggaccacttt
    16021 gccagtttct ccgaccaggc gggagaattt cgctgtcagg tcgccggtgc caccagccag
    16081 atccagcacg gtctgcccac ggcgtacgcc gctgcaatca atcgtgaatc gcttccacaa
    16141 acgatgaata ccaaatgaca tcaaatcatt catgacatcg tattttgatg ccacggaatg
    16201 gaaaacgtgg gcgaccatat ccgctttttg ttccttcgcg acggtctgaa aaccaaagtg
    16261 cgtcgtttct tgtgacttat ccaccatctc aatgcctgct catcaaaaaa ttgttccaga
    16321 agtgtaacag attgggcgtc gatgccctag atttctaccc ggcttaacta cccctaatgg
    16381 gctagcgcga ctgctgatta tattcatcat cgcgttgata agcttcatca ttcggctgct
    16441 ccggaaccga ccgaagtcga tactcttcat tctggctcac cgcctgttca gccaaatccg
    16501 gattaatctc gcgtttaatt tctactccta aaccgcgaaa cgcttctgcc tgcgccagca
    16561 catttccgcg acctgaagag agttttttca ttgcctggcg gtagttatcc tgcgctttgt
    16621 cgagactttg accaatcgcg gacatgtcat cgatgaacaa acgcatcttg tcgtacagct
    16681 tgctggcacg atcggcgatt tgctgggcgt tgcggctttg atgctcataa cgccacaggt
    16741 tggcaatagt acgcaacgcc accagcagcg tagtcgggct aaccagcatg atattgtttt
    16801 tcaacgcttc ggtgatcagc tccggctggc ggtcaagcgc cagtaaaaaa gcgggttcaa
    16861 cgggaataaa catcagcacg taatccagag ttcgcagccc cggcagctgt tgataatctt
    16921 tgcgtcccag caaacggata tggttacgca ccgacgcgat atgttcctgt agcgcgcttt
    16981 cgcgggtgta gtcgtcttcg gcgttaaaat agcgttcata ggcgaccagc gtcattttgg
    17041 cgtcgatcac cacatctttt ccctgcggca gccgcacgat gacatccggc tgcatccgcg
    17101 agcgggcgtc attttcgatg ctgacctggg tttcatattc atacccttca cgcagaccgg
    17161 aagcctccag cacccgcgtc aatactacct cgccccagtt gccctgggtt ttattgtcgc
    17221 ctttcagcgc gcgcgtcagg ttgatcgctt cctgggccat ttgcgcgttg agttgctgga
    17281 gattgcgaat ttcgtgggtc agggtatggc gttcttgtgc ttctttaccg aagctgtcct
    17341 gaacctgacg gcggaaaccg tccagttgtt cacgtagcgg cgacaacagg ctgttcagac
    17401 tctgacggtt ttgctcatca acccggcgat tgctgtgctc aaaaatacgg ttggcgaggt
    17461 tttcaaactg ctcactgagg cgctgctcgc tgttaatcat ctggcgaatt ttatcgtcag
    17521 catgttgctg tgcggcttcc atccgcgtgg ttacttcacg cagatcggcc tccagagagg
    17581 tgttaatact ttgcaggctg cgcacttcgt tattgagtaa ctcgcactct gcacgccagt
    17641 gctcgctttg ggtaatttgt tgttttgccg cgcttaactc cgcgaccatc tcttcacgtt
    17701 cagctaattg ctcggctttt tgctgcgcat gttgataact ggcaaacagc cagccaattg
    17761 ccacacccac caacgcaata actgcgtaaa ccatgattga gaaatccaca acgcctcctg
    17821 acgcaacaac ttacccgcac aaaatagaat tgtgctgtac aaacgtccag ttggaaaggc
    17881 atttcctgcg aggcgctacg caaaaataca aaaggccgaa cgcgtcggcc ttcagacagg
    17941 agaagagaat tacagcagac gacgcgccgc ttccaccacg attttcaccg cttggctttc
    18001 agtttgtttc atcgtctcag cattcgggat ctcttgctgg gtgcggttaa cgataacacc
    18061 cgctaccata ccggcacgca ggccctgact tgcacacatg gtcagcaggg ttgcagattc
    18121 catttcatag ttcattatgc ccatcgcctg ccactcttcc atagaacctt taaagtgacg
    18181 aactacgcga ccagagtaag tatcgtaacg ttcctgacct gggtagaagg tatcagaaga
    18241 agctgtcacg ccaacgtgag ttgtcgcgcc aatggatttc gcagcttcaa ccagcgcagt
    18301 cgtacattcg aaatcagcga cagccgggaa ttccagcggt gcgaagtgca ggctcgcgcc
    18361 atccagacgg acagacgccg tggtaaccag gacatcaccc acattaatat gcggctgaat
    18421 agcgcccgtt gtaccgatac gcaggaaggt gcgaatgccc agctgtgcca gctcttcaac
    18481 agcaatagag gtagacgggc cgccgatacc ggtagagcag acgataacag gtttaccatc
    18541 cagctctgca cgccaggtag tgaattcgcg gtgagatgcc agcttaaccg gcttatccat
    18601 cagcgcggcg atcttttcca cacgatccgg gtcgccaggg acgatggcaa gcgtagcccc
    18661 ttgtaaatcg tttttagtga ggccgagatg aaaaacatca gacttggaca tatacaactc
    18721 ctctgtgaat cggtttagtc agaagaagca aaaagacact ttaccgaagg gtttaacatt
    18781 ttttcgtgat actcatcacc atgacgcaaa tgcgttgcat aaatttaccc accaaaagtg
    18841 attcaaatca cataaatcac tcttcattct gcaggactgc ataaaagatg agccgattca
    18901 ggcaatgtga tttgttgcat gctgactata gttaacattg cgcaacattt ttataagggt
    18961 acggagaata ccatggcaac aacacaacaa tctggatttg cacctgctgc atcgcctctc
    19021 gcttcgacca tcgttcagac cctggacgac gcgattgtgg cgggcttcac ctctatccct
    19081 tcacaagggg ataacatgcc tgcttaccat gccagaccaa agcaaagcga tggcccactg
    19141 ccagtggtca ttgtagtgca ggaaattttt ggcgtgcatg aacatatccg cgacatttgt
    19201 cgccgtctgg cgctggaggg gtatctggct atcgcacctg aactttactt ccgcgaaggc
    19261 gatccgaatg attttgccga tatccccacg ttgcttagcg gtctggtagc aaaagtgcct
    19321 gactcgcagg tgctggccga tctcgatcat gccgccagtt gggcttcccg caacggcggc
    19381 gatgttcatc gtttaatgat caccggattc tgctggggtg gacgtatcac ctggctgtat
    19441 gccgcgcata atccacagct aaaagctgca gtggcgtggt acggcaaact gacgggcgac
    19501 aagtcgctga attcaccgaa acaacctgtt gatatcgcaa ccgatcttaa cgcgccgatt
    19561 ctcggcttat atggcggtca ggataacagc attccgcagg agagcgttga aaccatgcgc
    19621 caggcgctgc gggctgctaa tgcgaaagca gagattatcg tgtacccgga tgccgggcat
    19681 gcattcaacg ctgattatcg cccgagctat catgccgaat ctgcgaaaga tggctggcag
    19741 cgtatgctgg aatggtttaa gcagtatgga gagaagcgga gttagtttcc tcttagtcta
    19801 tatttgagat ggtgagtaaa cgagtgcgtt ttgctgccat ctctgtcccc caaaaaataa
    19861 tcagtatgaa ttgattggag attacgactc cgctatttga ccttaagaaa gtcggcaagt
    19921 catagtcata aatgagtctc ttcaatggaa gacaccttag agatttacca tcaactccca
    19981 ttcacaaaca aacattaatt attatcaaca cattcacatt aattataaat catctcagac
    20041 cttcctgata aaaagatatt aaagtgaaac attttgtttt tcacatttta gttcatcact
    20101 tttaggcgta aataaaggta aaaaaaacaa caatactatt tctctttcac aaaagaatta
    20161 acaaaaggtt tttcaaggat gagtgatatt atttacctga ccataaccgg agaacaacaa
    20221 ggatgtattt cctctcgttg tggaacgagc gcatcaatag gtaatcgctg gcagattggc
    20281 catgatgatg aaatcttcgc gttctcgctc tctaatagta ttacgaatac aggcaaaggg
    20341 tcataattgc atggccttag tttctgtaaa ttgattgaca aaagctcccc gttacttatt
    20401 aacgctatta acaataacga gcagttgttc atggagtttg acttctatcg gatcaatagg
    20461 tttggtcgat gggagaaata ttacaacatt caactccgcg gcgcgctctt atcagcaatc
    20521 aatcacctgt ttactgagaa caatctggat acagaagcca tcactgtcag ttattaatat
    20581 atactctcca ggcatctgat tgctaatact gaatttagtt attgggcatt tcccgataac
    20641 tacaaccgtc tgtttatacc acggccaaaa actaaagctg gtaatggttt caaaacactt
    20701 aatagtaaag cggttggcag actgcttgct gctgtcggca tatacaatgg gaatatcgaa
    20761 ggattcaggg aaacagcaga caagttgggg ggagatgcac ctgctggtta tgatcaattt
    20821 atggataaca aagggttact catcgctggt gcttcaatag ttgctggagc agttctgggg
    20881 agaatgagac tccctgattt ggaatcactt gagcatttcg gagccagagg agcagtatca
    20941 ggaagaccat ttaatcccga gcttgccggt ggtccaatag aaaacctgac gacagatggc
    21001 gtaacaatca acagggaagg tatcgctatt gttgaaaaac atatagccag atttggtcat
    21061 gatcctgtaa atgaagtgat gatcaataga ctgaaagaca ttgagaaagg aaaaatcccc
    21121 ccagaacaag tagatcttaa cttctatact catgagtgtc gggaatatca gcgttactgt
    21181 aatcttggtt gggaaacagg acaacctgat ggtgacgcag gctatgcact ctggaatca
//
LOCUS       DAJTYZ010000071        21039 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000071 DAJTYZ010000000
VERSION     DAJTYZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21039
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.071"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..379)
                     /gene="cmtA"
                     /locus_tag="OO596_002468"
     CDS             complement(<1..379)
                     /gene="cmtA"
                     /locus_tag="OO596_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR6718304.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAG"
     gene            complement(407..850)
                     /gene="cmtB"
                     /locus_tag="OO596_002469"
     CDS             complement(407..850)
                     /gene="cmtB"
                     /locus_tag="OO596_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR6718305.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1164..3155)
                     /gene="tkt"
                     /locus_tag="OO596_002470"
     CDS             complement(1164..3155)
                     /gene="tkt"
                     /locus_tag="OO596_002470"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6718306.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            3433..4191
                     /gene="loiP"
                     /locus_tag="OO596_002471"
                     /gene_synonym="yggG"
     CDS             3433..4191
                     /gene="loiP"
                     /locus_tag="OO596_002471"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6718307.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4334..5254)
                     /gene="speB"
                     /locus_tag="OO596_002472"
     CDS             complement(4334..5254)
                     /gene="speB"
                     /locus_tag="OO596_002472"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6718308.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5392..7368)
                     /gene="speA"
                     /locus_tag="OO596_002473"
     CDS             complement(5392..7368)
                     /gene="speA"
                     /locus_tag="OO596_002473"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6718309.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7377..7508)
                     /gene="yqgB"
                     /locus_tag="OO596_002474"
     CDS             complement(7377..7508)
                     /gene="yqgB"
                     /locus_tag="OO596_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6718310.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            7644..7859
                     /locus_tag="OO596_002475"
     CDS             7644..7859
                     /locus_tag="OO596_002475"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718311.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8163..9317
                     /gene="metK"
                     /locus_tag="OO596_002476"
     CDS             8163..9317
                     /gene="metK"
                     /locus_tag="OO596_002476"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6718312.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            9753..11147
                     /gene="galP"
                     /locus_tag="OO596_002477"
     CDS             9753..11147
                     /gene="galP"
                     /locus_tag="OO596_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6718313.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11224..11721
                     /locus_tag="OO596_002478"
     CDS             11224..11721
                     /locus_tag="OO596_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6718314.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            11816..12523
                     /gene="endA"
                     /locus_tag="OO596_002479"
     CDS             11816..12523
                     /gene="endA"
                     /locus_tag="OO596_002479"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6718315.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            12603..13334
                     /gene="rsmE"
                     /locus_tag="OO596_002480"
     CDS             12603..13334
                     /gene="rsmE"
                     /locus_tag="OO596_002480"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6718316.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13347..14297
                     /gene="gshB"
                     /locus_tag="OO596_002481"
     CDS             13347..14297
                     /gene="gshB"
                     /locus_tag="OO596_002481"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6718317.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            14406..14969
                     /locus_tag="OO596_002482"
     CDS             14406..14969
                     /locus_tag="OO596_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6718318.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            14969..15385
                     /gene="ruvX"
                     /locus_tag="OO596_002483"
     CDS             14969..15385
                     /gene="ruvX"
                     /locus_tag="OO596_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6718319.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(15560..16540)
                     /locus_tag="OO596_002484"
     CDS             complement(15560..16540)
                     /locus_tag="OO596_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6718320.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            16558..17262
                     /gene="yggS"
                     /locus_tag="OO596_002485"
     CDS             16558..17262
                     /gene="yggS"
                     /locus_tag="OO596_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6718321.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17280..17846
                     /gene="yggT"
                     /locus_tag="OO596_002486"
     CDS             17280..17846
                     /gene="yggT"
                     /locus_tag="OO596_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6718322.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            17843..18133
                     /locus_tag="OO596_002487"
     CDS             17843..18133
                     /locus_tag="OO596_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6718323.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18141..18734
                     /locus_tag="OO596_002488"
     CDS             18141..18734
                     /locus_tag="OO596_002488"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6718324.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            18727..19863
                     /gene="hemW"
                     /locus_tag="OO596_002489"
     CDS             18727..19863
                     /gene="hemW"
                     /locus_tag="OO596_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6718325.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20174..21039
                     /locus_tag="OO596_002490"
                     /pseudo
     CDS             20174..21039
                     /locus_tag="OO596_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         5172 a         5462 c         5374 g         5031 t
ORIGIN      
        1 aaccggcagg tatgcgtttt tccagtgcct tatcaacata ttttatgacc agcccaccga
       61 gcggccccat aatcattgag ccaaggaaca tcgggatctc tgcgccgacg atcacaccaa
      121 tagtacctat tccgcccatg accgcgccgc gtttaccgcc gaccagatga ccaccggtag
      181 aaccaatcat cacgggcaat aaataggtaa tcatcgggcc gacaattttg gcgaaatgtt
      241 cgttaggcag ccaaccggtg ggaataaata acgcagtaat aaaaccccag gcaataaaag
      301 caccaatatt ggggatgacc attgccgtca aaaagccccc aaaagcctgg acctttgcac
      361 gagcagactt gttttccata atattatcct gtagaggaga gagtaattat ccgcggctga
      421 ttatgtccgc taattgtttt tctgatgctg ctgttaagag ttgttcgagt atttcttctt
      481 cgcacagtaa ttcacttaac gcctgaatag cgccaatgtg cgaatcggca tcggcagcag
      541 aaaggccgat gagtaatttt attggttcgt cattccccgg aaaataaaca ccttgttcaa
      601 gtaatgtcaa agacatcccg gttttaagcg ccccacattc cggtcgcgca tgaggcattg
      661 ccacgcctgg tgcgagaata taataagggc cattgttaat ggtggaatct ttaattgcct
      721 gaatgtaatt ctcgctgata tagtttttat ccagcaatga taccatcgag aaatcgatag
      781 cttcctgcca atcttttgct gaatgtataa ctgagattga tgattctgga aaatagtcac
      841 taagccgcat aaattatcct tattttattt ttagggtacc gtccggcggt aaaaataaaa
      901 tactcgtgcg gtttaatgaa agcaattgag cagggaaacc ggtgttttga aaacagtgaa
      961 ttattaattt cttatataac attgagttat agatataaca acaaatgtca atacgcatat
     1021 cgtgatgcat attacataat tgatatttat tgcatataaa tatttgtgat ctacaacacg
     1081 ctttatctat tgcttgtccg caaacggaca tatcaaggta ataaaaaagg tcgccgaagc
     1141 gaccttttta cccgaaatgc taattacagc agttcttttg ctttcgcaac gacgttgtcg
     1201 acggtgaagc cgaactcttc gaacagcaac tctgccggag ctgactcacc gaaggtggtc
     1261 ataccgatga tagcgccatt caggccaaca tacttgtacc agtagtcagc aatacccgct
     1321 tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta agccgcatcc
     1381 tgcttgtcga atgcatcggt agacggcatg gaaaccacgc gcgccttcac gccttcggca
     1441 gtcagttttt cgtaggcggc aacagccagt tcaacttccg aaccggtcgc gatgaagatc
     1501 agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat gtttgccagt
     1561 tgctcttcag tacgttcctg ctgcgccaga ttctgacggg agaggataag cgcggtcgga
     1621 ccgtcctgac gctcaacacc gtatttccac gcgaccgcgg attcaacctg gtcacacgga
     1681 cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctcaac cggctggtga
     1741 gtcgggccgt cttcgcccag accgatggag tcgtgggtgt aaaccatcac ctgacgctgt
     1801 ttcatcagcg cagccatacg tacagcgtta cgtgcgtatt cgacaaacat caggaaggtg
     1861 gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc ggtcataccg
     1921 aactcgcgaa caccgtagtg gatgtagtta cccgcagcat cttcgttgat tgctttagaa
     1981 ccagaccaca gggtcaggtt agacggtgcc aggtcagcgg agccgccgag gaattccggc
     2041 aacagcgggc cgaacgcttc aatagcattc tgagacgctt tacggctggc gattttcgcc
     2101 ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc agacggcatt
     2161 tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc
     2221 gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc atcccactgc
     2281 gcatagattt cagacgggat ttcgaacggt gcgtatttcc agcccagctg ttcgcgggtc
     2341 agagcgattt ctgcatcacc cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc
     2401 ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc agtcactgcg
     2461 cgcgcttctt ctactgcgcg tttgatggat gctgcgtcat gaccgtcgat gtcgcgaata
     2521 acgtgccagc cgtaagcttc gaaacgcatt gcggtgtcgt cggtgaacca gccttcaacg
     2581 tgaccatcaa tagagatacc gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc
     2641 gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg
     2701 aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg cgccgccagc
     2761 gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag cggaccggtg
     2821 gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt agagtgcagc
     2881 tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg cagcaggctg
     2941 tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc agcccaggac
     3001 ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat gtcagccata
     3061 cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat gctcagcgca
     3121 cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg gatgatgaag
     3181 ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa atttgccaac
     3241 aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt cagaaatctc
     3301 tggatcatgc tcgcatgttg cgcaatctac tcgcccgtcc gctgcgcttt tccttatact
     3361 gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct gaccttgtgc
     3421 ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg
     3481 actggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga agcttttcag
     3541 gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg tcaggagatg
     3601 gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct gacaactatt
     3661 gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt gtatatggcg
     3721 aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag cgggctgatg
     3781 gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg
     3841 ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt gcgagtagct
     3901 gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg tgatctgggc
     3961 gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga tgattattct
     4021 tacgatcttc tgcgccaacg cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa
     4081 ctggcgaaac tggaagaagg ccgccaaagc tcaatgtttg acgaccatcc ggcatccgcc
     4141 gaacgcgccc agcatattcg cgatcgcatg agcgcggatg ggattaagta aagcctggtg
     4201 gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacatcaatg gcacatttgt
     4261 taccctgtgc gcattgccgg atgcgatgct gactgcatct tatccggcct acgggtggca
     4321 tcgcatctgg tgcttactcg ccctttttcg ccgcttggat atacagcatc tccagcgcca
     4381 gcgtcgccgc agccagagcg gtgatttccg actgatcgta tgccggagcc acttccacta
     4441 cgtccatccc aacaatgttg agatctttca ggccgcgtac cagtttaata gcgcgatcgg
     4501 aggtcaggcc gccaatcact ggcgtaccgg tgcctggtgc aaaagcagga tccaggcagt
     4561 cgatatcaaa agtcaggtaa accggcatat cacccacaat ctgtttcact tgggcgataa
     4621 cgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag ccgttgtctt
     4681 tatcaaactc ggtacgaata ccaatctgca cggaatgatt cgggtcgatc agaccttctt
     4741 tcggcgcggt atagaacata gtgccgtggt caaattcaca accgttcgca taggtatcgg
     4801 tgtgggcgtc aaagtgtacc agcgccattt tgccgaaatg cttcgcatga gcacgcagca
     4861 gcggcagcgt aacaaagtgg tcaccaccga aggtgagcat acgcttaccg gcagccagca
     4921 gcttctcggc gtgcgcctgc agcttttcgc tcatctcacg ggcatcgcca aaggcatata
     4981 ccagatcgcc gcagtccacg acgttcagac gctcacgcat gtcgaaattc cacgggaagc
     5041 ggttgtgttc ccaggccaga ttcgtcgaaa cctgacggat cgctgccgga ccgtggcgac
     5101 cacccgcacg accagaagtg gccatatcga acggcacgcc agtaatcacc cagtctgcat
     5161 cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggca ttggaaacca
     5221 gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctatta caaagtcgat
     5281 ataaaaaaac ccttccgcgt cgttaggccc gacgaggaag ggttggattt gtcacaataa
     5341 attgtggcgg attatcaccg ctaaattaag cggattcaag taacacagga cttactcatc
     5401 ttcaagataa gtataaccgt acaaacctgc ctcgaactct tcaaggaact gttgttgcag
     5461 ttcagcatca agatcggttt tcttcacttg atcgcggaac tgggttaaca gcgttttcgg
     5521 atcgagctgt acatattgca gcatgtccgc cacggtatcg ccttcgtcag acagttctac
     5581 ttctacgcta ccgtcaggga acacgaacac gtcaaccgct tcggtatcac cgaacaggtt
     5641 gtgcatattg ccaaggatct cctgatatgc gccgaccata aagaaaccga gcatcggcgg
     5701 attctctgga tcgtactccg gcattggcat tgtcgtggca ataccatcac catcaatata
     5761 gtggtcgata gcaccgtcag agtcacaggt aatatccagc agcacagcgc gacgttccgg
     5821 cacttgatcc agcccttcca gcggcagaac cgggaacaac tggtcgatcc cccatgcgtc
     5881 cggcattgac tggaacagcg agaaattgac gtacattttg tccgccatac gttcctgcag
     5941 ttcgtcgatt atcggacgat gcgcacggtt ttgcggatcc agttgcttct gcacttcatg
     6001 gcacatgctc agatagagtt gttctgccca tgcacgttct tgcaggctaa aggtgccgga
     6061 agagtagccg atatgaatgt cgtgcagatc catctggctg tcgtgtaacc attcacgcaa
     6121 agaacggcga gttcccggtt cgtgcatttc ctgccaggtt tcccacatgc tttgcagcgc
     6181 gcgcggcgca tcttctgcgg gcgcggtcgg caccgtatat tcgttacgtt ccacgccgat
     6241 gatattcgac accagcacgg tatggtgcgc agtcaccgca cggcccgatt cggtgattac
     6301 cgtcggatgc ggcagaccat tttcttcaca cgcatcgcca atcgcccaga tgatgttgtt
     6361 ggcgtattca ttgaggccat agtttacgga gcagtcagac tgcgaacggg tgccttcata
     6421 gtccacgccc agaccgccgc cgacgtcgaa gcactgaata ttgacgccca gcttgtgcag
     6481 ttccacatag aaacgcgcgg attcacgaac gcccgtggcg atatcgcgaa tattcgccat
     6541 ctgcgaaccg aggtggaagt gcagtagttg caggctgtcg agacgcccgg cttcacgcag
     6601 ggtttcaacc agttgcagta cctgagtcgc agccaggccg aacttcgatt tttccccgcc
     6661 ggaggactgc catttacccg agccctgcga agccagacgt gcacgcacgc ccagacgagg
     6721 aacgacattc agacgttctg cttcatccag cacaatggcg atttctgaca tcttctcaat
     6781 gaccagatag accttgtgcc ccatcttctc gccaattaat gccaggcgga tatattcgcg
     6841 gtctttataa ccgttgcaga cgatgacgct acgggtcatg ccagcatgtg ccagtactgc
     6901 catcaactcg gctttggaac cggcttccag acccagcggt tcgcccgaat gaatcaggga
     6961 ctcaatcacg cggcggtgct ggttaacttt gatcggataa acaaggaagt aatcgccgtt
     7021 atagccgtag gattccctcg cacgtttgaa cgcggcgtta atggaacgca aacggtgctg
     7081 caggatctgt gggaaacaga acagtgcagg cagacgctga ccctgtgctt cacgagtttt
     7141 cactaactgc gcgagatcga cgcgagcttc cgggacgtcc gggtccgggc acacgctgat
     7201 gtggcccagc tcgttaacgt catagtagtt attgccccac caggcaatat tgtaagtacg
     7261 cagcatcttg ctggcttcct gggagctcat tgcaacctcc tgcatggagc gtagtacacc
     7321 gtgttcgccc gctgacgaag gcaaacccat agacatgtcg tcagacatag cgaacctcaa
     7381 attattttat taagtgtaaa acagttaacg actatcgcag cctggaactg cgataacaac
     7441 ccataagcac atggattttc cagcagtgaa tgctgacgct ccaactgcgc aaccggtttc
     7501 tttttcataa cattattaag cacataaccg aacgtaagtg tgacagttcg gcgaaaccac
     7561 gagaaaactc ttgtttttac aagagcgccc ttgttcagtc ctcagtaact gtaaccagct
     7621 cttgaatcct gagaagcgcc gagatgggta taacatcggc aggtatgcaa agcagagatg
     7681 cagagtgcgg ggaacgaatc ttcaccagaa cggtgagaca ggttaagcag cagacaacgg
     7741 ttcattattt cgtatcacct ccacggccgc ctgttaagac gaacccacaa gccaaaactc
     7801 tgatttcaac ccggctggaa gtggcaacac gaaagaaacg tcgtgtgctt tttatttaag
     7861 ccgcgcgccg cgttttatac cccacaatgg cagaaaattg caaaagataa atacgcagaa
     7921 tgccggcatt gtcaggaaaa atttccagcc acgtttttaa cagaatgaga cacgattcaa
     7981 aaaaaagtgg aaatcgggtg aagaattgac ctaaaatagc catccagatg ttaatccatc
     8041 cataccgatt aacactcaga ctgccagtgt ttttaacctg cagagtcgtg gtaggatccg
     8101 ctaccacaga aaatccacac aacagtttga gctaaccaaa ttctctttag gtgatattaa
     8161 atatggcaaa acaccttttt acgtccgagt ccgtctctga agggcatcct gacaaaattg
     8221 ctgaccaaat ctctgatgcc gttttagacg cgatcctcga acaggatccg aaagcacgcg
     8281 ttgcttgcga aacctacgta aaaaccggca tggttttagt tggcggcgaa atcaccacca
     8341 gcgcctgggt agacatcgaa gagatcaccc gtaacaccgt tcgcgaaatt ggctatgtgc
     8401 attccgacat gggctttgac gctaactcct gtgcggttct gagcgctatc ggcaaacagt
     8461 ctcctgacat caaccagggc gttgaccgtg ccgatccgct ggaacagggc gcgggtgacc
     8521 agggtctgat gtttggctac gcaactaatg aaaccgacgt gctgatgcca gcacctatca
     8581 cctatgcaca ccgtctggta cagcgtcagg ctgaagtgcg taaaaacggc actctgccgt
     8641 ggctgcgccc ggacgcgaaa agccaggtga ctttccagta tgacgacggc aaaatcgttg
     8701 gtatcgatgc tgtcgtgctt tccactcagc actctgaaga gatcgaccag aaatcgctgc
     8761 aagaagcggt aatggaagag atcatcaagc cgattctgcc cgctgaatgg ctgacttctg
     8821 ccaccaaatt cttcatcaac ccgaccggtc gttttgttat cggtggcccg atgggtgact
     8881 gcggtctgac tggtcgtaaa attatcgttg atacctacgg cggcatggca cgtcacggtg
     8941 gcggagcatt ctctggtaaa gatccatcaa aagtggaccg ttccgcagcc tacgcagcac
     9001 gttatgtcgc gaaaaacatc gttgctgctg gcctggccga tcgttgtgaa attcaggttt
     9061 cctacgcaat cggcgtggct gaaccgacct ccattatggt agaaactttc ggtactgaga
     9121 aagtgccttc tgaacaactg actctgctgg tacgtgagtt cttcgacctg cgcccatacg
     9181 gtctgattca gatgctggat ctgctgcacc cgatctacaa agaaaccgca gcatacggtc
     9241 actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa agcgcagctg ctgcgcgatg
     9301 ctgccggtct gaagtaatct ttcttcacct gctttcaaag gccagcctcg cgctggcctt
     9361 tttcttttac ctcaccgcgg tggataggca ttcacgccgc atccggcaaa aagctgcccg
     9421 cacaataaca tcattcttcc tcaacacgtt ttatcgcaaa ttatcatcac tactgaaacc
     9481 gattacacca ataacaccag ccaaatattt gtaatatttt catattatta ttcagttttc
     9541 acagttgtta catttctttt cagtaaagtc ttaattgcag ataacagcgt ttaatctatg
     9601 atgatataac tcaattattt tcatgcactt aaaccataac taagataaat gttagtgtaa
     9661 gcgattacac tgatgtgatt tgcttcacat ctttttacgt cgtactcacc tatcttaatt
     9721 cacaataaaa aataaccata ttggagggca tcatgcctga cgctaaaaaa caggggcggt
     9781 caaacaaggc aatgacgttt ttcgtctgct tccttgccgc tctggcggga ttactctttg
     9841 gcctggatat cggtgtaatt gctggcgcac tgccgtttat tgcagatgaa ttccagatta
     9901 cttcgcacac gcaagaatgg gtcgtaagct ccatgatgtt cggtgcggca gtcggtgcgg
     9961 tgggcagcgg ctggctctcc tttaaactcg ggcgcaaaaa gagcctgatg atcggcgcaa
    10021 ttttgtttgt tgccggttcg ctgttctctg cggctgcgcc aaacgttgaa gtactgattc
    10081 tttcccgcgt tctactgggg ctggcggtgg gtgtggcctc ttataccgca ccgctgtacc
    10141 tctctgaaat tgcgccggaa aaaattcgtg gcagtatgat ctcgatgtat cagttgatga
    10201 tcactatcgg gatcctcggt gcttatcttt ctgataccgc cttcagctac accggtgcat
    10261 ggcgctggat gctgggtgtg attatcatcc cggcaatttt gctgctgatt ggtgtcttct
    10321 tcctgccaga cagcccacgt tggtttgccg ccaaacgccg ttttgttgat gccgaacgcg
    10381 tgctgctacg cctgcgtgac accagcgcgg aagcgaaacg cgaactggat gaaatccgtg
    10441 aaagtttgca ggttaaacag agtggctggg cgctgtttaa agagaacagc aacttccgcc
    10501 gcgcggtgtt ccttggcgta ctgttgcagg taatgcagca attcaccggg atgaacgtca
    10561 tcatgtatta cgcgccgaaa atcttcgaac tggcgggtta taccaacacc acagagcaaa
    10621 tgtgggggac agtgattgtc ggcctgacca acgtacttgc cacctttatc gcaatcggcc
    10681 ttgttgaccg ctggggacga aaaccaacgc taacgctggg cttcctggtg atggctgctg
    10741 gcatgggcgt actcggtaca atgatgcata tcggtatcca ctctccgtcg gcgcagtatt
    10801 tcgccatcgc catgctgctg atgtttattg tcggttttgc catgagtgcc ggtccgctga
    10861 tttgggtact gtgctccgaa attcagccgc tgaaaggccg cgattttggc atcacctgct
    10921 ccactgccac caactggatt gccaacatga tcgttggcgc aacgttcctg accatgctca
    10981 acacgctggg taacgccaac accttctggg tgtatgcggc tctgaacgta ctgtttatcc
    11041 tgctgacatt gtggctggta ccggaaacca aacacgtttc gctggaacat attgaacgta
    11101 acctgatgaa aggtcgtaaa ctgcgcgaaa tcggcgctca cgattaatct cctcaggctt
    11161 cctcccgtcg cggaggaagc cgcctcttgc agtcatcttt tcttcgctct atcctctgcc
    11221 gctatgaaaa catcccgtct ccctatcgcc atccaacagg ccgttatgcg cggcctgcgg
    11281 gaaaaactcg cccaggccaa cctgaagcta gggcgtaact acccggagcc aaaactctct
    11341 tacacccagc gcggaacctc cgccggaaca gcctggctgg aaagctatga aatccgcctc
    11401 aatcccgttt tgctgttgga aaacagtgaa gcttttattg aagaagtggt accgcacgaa
    11461 ctggcacatt tgctggtatg gaaacatttc ggccgcgtag cgccacatgg caaagagtgg
    11521 aagtggatga tggaaagcgt gctgggtgtt cccgcccgtc gtacgcatca gttcgaactg
    11581 caatccgtgc gtcgcaacac cttcccctac cgctgcaagt gccaggagca tcagcttacc
    11641 gtacgccgcc ataatcgcgt agttcgtggc gaggccgtct atcgctgtgt tcactgcggt
    11701 gaacagctgg ttgcgaaata accatctgaa ctatcaggaa ctttcctgat ctggctgatt
    11761 gcataccaaa acaactttcg ctacgttgct ggctcgtttt aacacggagt aagtgatgta
    11821 ccgttatttg tctattgctg cggtggtact gagcgcagca ttttccggcc cggcgttggc
    11881 cgaaggtatc aatagttttt ctcaggcgaa agccgcggcg gtaaaagtcc acgctgacgc
    11941 gcccggtacg ttttattgcg gatgtaaaat taactggcag ggcaaaaaag gcgttgttga
    12001 tctgcaatcg tgcggctatc aggtgcgcaa aaatgaaaac cgagccagcc gcgtagagtg
    12061 ggaacacgtc gttcccgcct ggcagttcgg tcaccagcgc cagtgctggc aggacggtgg
    12121 acgtaaaaac tgcgctaaag atccggtcta tcgcaagatg gaaagcgata tgtataacct
    12181 gcagccgtca gtcggtgagg tgaatggcga tcgcggcaac tttatgtaca gccagtggaa
    12241 tggcggcgaa ggccagtacg gtcaatgcgc catgaaggtc gatttcaaag aaaaagctgc
    12301 cgaaccacca gcgcgtgcac gcggtgccat tgcgcgcacc tacttctata tgcgcgacca
    12361 atacaacctg acactctctc gccagcaaac gcagctgttc aacgcatgga acaagatgta
    12421 tccggttacc gactgggagt gcgagcgcga tgaacgcatc gcgaaggtgc aaggcaatca
    12481 taacccgtat gtgcaacgcg cttgccaggc gcgaaagagc taacctacac tagcgggatt
    12541 ctttttgtta acccctaccc cacgcgtaca accgcgtggg gagacgacgc ggatttttaa
    12601 ctatgcgtat cccccgcatt tatcatcctg aaccactgac cagccattct cacatcgcgc
    12661 tttgcgaaga tgccgccaac catatcgggc gcgtactgcg catggggccg gggcaggcgt
    12721 tgcaattgtt tgacggtagc aaccaggtct ttgacgccga aattaccagc gccagcaaaa
    12781 aaagcgtgga agtgaaggtg ctggaaggca agatcgacga tcgcgaatct ccgctgcata
    12841 ttcacctcgg tcaggtgatg tcgcgtggtg aaaaaatgga atttacgatc cagaaatcga
    12901 tcgaactcgg tgtaagcctc attacgccac ttttttctga gcgctgcggc gttaaactgg
    12961 atagtgaacg tctgaacaag aagctccagc agtggcagaa gattgcaatt gctgcctgtg
    13021 agcagtgtgg tcgtaaccgg gtgccggaaa tccgtccagc gatggatctg gaagcctggt
    13081 gtgcagagca ggatgaagga ctgaaactga atcttcaccc gcgcgccagt aacagcatca
    13141 atacgttgcc gttaccggtt gaacgcgtcc gcctgctgat tggcccggaa ggcggtttat
    13201 cggcagatga aattgccatg actgcccgct atcaatttac tgatatcctg ttgggacctc
    13261 gcgttttgcg tacagagaca actgcgctca ccgccattac cgcgctacaa gtacgatttg
    13321 gcgatttggg ctaacggaga agaataatga tcaagctcgg catcgtgatg gaccccatcg
    13381 caaacatcaa catcaagaaa gattccagtt tcgctatgtt gctggaagca cagcgtcgtg
    13441 gttacgaact tcactatatg gagatgggcg atctgtatct gatcaatggc gaagcccgcg
    13501 cccatacccg cacgctgaac gtgaagcaga actacgaaga gtggttttcg ttcgtcggtg
    13561 aacaggatct gccgctggcc gatctcgatg tgatcctgat gcgtaaagac ccgccgtttg
    13621 ataccgagtt tatctacgcg acctatattc tggaacgtgc cgaagagaaa gggacgctga
    13681 tcgttaacaa gccgcagagc ctgcgcgact gtaacgagaa actgtttacc gcctggttct
    13741 ctgacttaac gccagaaacg ctggttacgc gcaataaagc gcagctaaaa gcgttctggg
    13801 agaaacacag cgacatcatt cttaagccgc tggacggtat gggcggcgcg tcgattttcc
    13861 gcgtgaaaga aggcgatcca aacctcggcg tgattgccga aaccctgact gagaatggca
    13921 ctcgctactg catggcacaa aattacctgc cagccattaa agatggcgac aaacgcgtgc
    13981 tggtggtgga tggcgagccg gtaccgtact gcctggcgcg tattccgcag gggggcgaaa
    14041 cccgtggcaa tctggctgcc ggtggtcgcg gtgaacctcg tccgctgacg gaaagtgact
    14101 ggaaaatcgc ccgtcagatc gggccgacgc tgaaagaaaa agggctgatt tttgttggtc
    14161 tggatatcat cggcgaccgt ctgactgaaa ttaacgtcac cagcccaacc tgtattcgtg
    14221 agattgaagc agagtttccg gtgtcgatca ccggaatgtt aatggatgcc atcgaagcac
    14281 gtttacagca gcagtaaccc accttagcga gaaggatctc gttgagactc tgagtgacag
    14341 cgcccttctt tccacgcata ctgggcgctg ttgctttttt gaaccaggaa acagaacctc
    14401 tgacaatgaa tttacagcat cactttctta ttgccatgcc tgctctccag gatccgattt
    14461 tccgtcgttc cgtggtctac atttgcgaac ataataccaa tggtgcaatg gggatcatcg
    14521 tcaacaagcc gctggaaaat ctcaaaattg aagggattct ggaaaagctg aagatcacgc
    14581 cggagccgcg tgatgaatca atccgtctgg ataaaccggt tatgctcggc ggtccgctgg
    14641 ctgaagatcg cgggtttatt ttgcatactc cgccctccaa ttttgcttcc agcattcgca
    14701 tttcagacaa cacagtgatg accacttccc gcgatgtgct ggaaacgctc ggcaccgata
    14761 aacaaccgtc tgacgtattg gtggctctag gttatgcctc ctgggaaaaa ggtcaactgg
    14821 aacaagaaat tctcgataac gcgtggctaa cggccccggc ggatctgaat attctgttca
    14881 aaacgccgat tgccgaccgc tggcgcgagg cggcaaaact gattggtgtg gatattctca
    14941 ccatgcctgg tgtggcaggg cacgcctgat gagtggaacc ttactcgcct tcgacttcgg
    15001 caccaaaagc attggcgtag cggtcggcca acgcattacc ggcactgctc gccctttgcc
    15061 tgcaattaaa gcacaggacg gtacgccgga ctggaacctc atcgaacgtt tactgaaaga
    15121 gtggcagccg gacgaaatca tcgtcggttt gccgctgaat atggacggca ccgagcaacc
    15181 attgactgcc agagcgcgta aatttgccaa ccgtattcat ggtcgtttcg gtgttgaagt
    15241 aaagctccat gacgaacgtc ttagcaccgt ggaagcccgt tccggtctgt ttgaacaggg
    15301 cggctatcgg gcgctcaaca aaggcaaagt cgactctgcc tctgcggtta ttattctcga
    15361 aagctatttc gagcagggat attaaggcga tttaaaagcc tggcggatag tcgttaccgc
    15421 catcttgccg gatgcggcgt gaacgcctat ccggcctaca tgtggcagcg attgtaggtc
    15481 tgataagacg cgcaaacgtc tcatcagacg tttattgcct ttgtaggccc gataagcttg
    15541 cacatcgggc atggcaacgt cacaaacgcc cttcccccac tcgctgctga aaactctgct
    15601 gaaacgttat catccccacc tgctgcccgg tttgaataac atgcggtaac tggtgggttt
    15661 tcccttcgcg aatcaaattc cccaccgcgg gagtgttaat caataattca aacagcgcca
    15721 cgcgtccttc ctgtttatcc acttccagct tttgtgacaa cacggcccgt aaactacctg
    15781 ccagttgatt acgtacggga tctttttcct gcgccggaaa tgaatccacc agtcgctcaa
    15841 ctgcctgcgc ggcaccacgc gtatgtaatg ttgccagcac caaatgcccg gtttctgccg
    15901 ccgtcagtgc cagacggatt gtctcgctgt cacgcagctc tccgagcaaa atcacatcag
    15961 gatcttcccg caatgcggcc cgcaatcccg atgcgaacgt catacagtgc aaaccaattt
    16021 cccgcttctg gatcaaacat cgctggctgg catagatata ttcaacagga tcttccagcg
    16081 tcagaatatg cgcatcggca tgttgattga gatagccaac catcgccgcc agcgtggtag
    16141 atttgctgct ccccgtcgcc cccgtcacca gaatcaggcc attctcactc ttgagtaatt
    16201 ccggcaatac cgttggtgcg ccaagctgtt cgagctgcgg gcagtgcgaa ggtaacagcc
    16261 gtaacgccag cgaaatgcca tgccgttgcg caaatgcgct gccacgcaat cgctggtttt
    16321 ccgccagcga cacggcaaaa tccagctggc cattttccag caatattgcc cgctgatcgt
    16381 catccagcca ctcccgcagt agctcttcga cgtccggcgc ctcaaacggc gcagcttcca
    16441 ttagcccgcg aatgcgccat cgtgcgggcc aggcgctgca caggtgtaga tccgagacgt
    16501 tatgctttac actaagggcc acaatttctt ccatattcat actaagatcc tcggaaaatg
    16561 aacgatattg cgcataacct ggcacaggtc cgggacaaaa tctcagcggc tgcaacgcgt
    16621 tgcggccgtt ctccagaaga aattacgctg cttgcagtca gtaaaacaaa acctgcgagc
    16681 gccatcgcag aagccattga tgccgggcag cgtcaatttg gtgaaaacta cgttcaggaa
    16741 ggggtagata aaattcgcca ctttcaggaa ctgggcgtaa caggattaga atggcatttt
    16801 attggcccgt tgcagtctaa taaaagccgc ctggtggcag agcatttcga ctggtgtcat
    16861 accatcgacc gtttgcgcat tgctacccgc ctcaacgatc agcgcccggc agaacttccc
    16921 cctcttaacg tactgattca aattaatatt agtgatgaaa acagtaagtc cgggattcaa
    16981 ctggcagaac tggacgagct ggcagcggcg gtcgctgaac taccgcgttt acgtctgcgc
    17041 gggctgatgg caatccctgc gcctgagtca gaatatgtaa ggcagtttga agttgcacgc
    17101 caaatggctg tagcatttgc cggactgaaa acgcgctacc cgcatatcga cacgctctct
    17161 ctgggaatgt cggacgatat ggaagccgcc attgcggcag gtagcacgat ggttcgtatc
    17221 ggcactgcaa tttttggtgc gcgtgattac tctaaaaaat aaggaattaa aggaacgcca
    17281 tgaatacgtt gactttcctg ctttcaacgg tcattgagct gtataccatg gtgctgttat
    17341 tacgcatctg gatgcagtgg gctcattgtg atttttacaa ccccttctca cagtttgtag
    17401 tgaaggtaac gcagccaatt atcgggccac tgcgccgcgt tattccggca atgggcccca
    17461 ttgacagcgc ctcgctgctg gttgcctata ttctcagttt tatcaaagcc atcgtgctgt
    17521 ttaaagtggt gaccttcctg ccaatcatct ggattgctgg tttactgatt ctgctgaaaa
    17581 ccatcggcct gctgattttc tgggtcctgc tggtgatggc gattatgagc tgggtaagcc
    17641 agggtcgtag tccgattgaa tacgtgctga ttcagctggc cgatccgctg ctgcgcccga
    17701 ttcgccgcct gctaccggca atgggtggga ttgatttctc gccgatgatc ctcgttctgc
    17761 tgctgtatgt catcaatatg ggtgtcgcag aagtattaca ggcaaccgga aacatgctgc
    17821 tgccggggct gtggatggcg ttatgagtgc cgtaacagtt aatgatgacg gtctggtttt
    17881 acggctctat attcagccga aagccagccg tgattctatt gtcggtttac atggcgacga
    17941 agttaaagtc gccattaccg cgccgccggt tgacggccag ggcaacagtc atctggtgaa
    18001 gtttctcggt aagcaattcc gggttgccaa aagccaggtg gtgattgaaa aaggcgaact
    18061 tggccgccac aaacaaatta aaatcattaa tccgcaacaa atcccgccag aaatcgcggc
    18121 gttaattaat taggtatcct atgcaaaaag ttgtcctcgc aaccggcaat gccggtaaag
    18181 tgcgtgagct ggcgtcgctg cttagcgact tcggtcttga tatcgtggcc caaacagacc
    18241 tcggcgtaga ttccgctgaa gaaaccggcc tgacctttat cgaaaacgcg attctgaaag
    18301 cgcgctatgc ggcaaaagtg accggtttac cggcaattgc cgacgactct ggtctggcgg
    18361 tagatgcgct gggtggcgcg ccgggtattt actccgcgcg ttattccggt gaagacgcga
    18421 ccgatcaaaa gaatctgcaa aaactgttgg aaacactgaa agacgtaccg gacgaccaac
    18481 gtcaggcgcg tttccactgc gtgctggtat atctgcgtca cgcggaagat ccgactccgc
    18541 tggtatgcca cggcagctgg ccgggcgtga ttactcgcga accagcgggc actggtggct
    18601 ttggttatga tccaatcttc ttcgtacctt ccgaagggaa aaccgccgcc gaactgaccc
    18661 gcgaagaaaa gagcgccatt tcccaccgtg gtcaggcgtt gaaactgctg ctggacgctt
    18721 tacgtaatgg ttaaattacc gccgctgagt ctctacattc acatcccgtg gtgcgtgcag
    18781 aaatgcccgt actgcgattt caactctcac gcgttgaaag gagaagtgcc gcacgacgat
    18841 tatgttcagc atctgctttg cgatctggac aatgatgtgg cttacgctca gggccgtgaa
    18901 gtaaagacaa tctttattgg cggtggtacg ccgagcctgc tttccggccc ggcgatgcaa
    18961 acgctgctgg acggcgtgcg tgcgcgtttg ccgctggcag cggatgcaga aattactatg
    19021 gaagcgaacc ctggaacggt agaagccgat cgctttgtcg attatcagcg tgctggtgtg
    19081 aaccgcatct ctattggtgt gcagagtttt agcgaagaaa agctgaaacg acttgggcgt
    19141 attcatggcc cgcaagaagc gaaacgcgcg gcaaagctgg cgagcgggct ggggctgcgt
    19201 agctttaacc tcgatttgat gcatggacta ccggatcaat cactggaaga ggcgcttggc
    19261 gatctacgcc aggccattga actgaatccg ccgcatcttt cctggtatca actgaccatc
    19321 gaacccaata cgctgtttgg ttcgcgacca ccggtgctgc cggacgatga cgcgctgtgg
    19381 gatattttcg aacaggggca tcagttatta accgcagcgg gttatcagca atatgaaact
    19441 tccgcttacg ccaaacccgg ttatcagtgc cagcacaatc tcaactactg gcgatttggt
    19501 gactacatcg gtattggctg cggcgcgcac ggcaaagtca ccttcccgga tgggcgcatt
    19561 ctgcgtacca ctaaaacgcg tcatcctcgt ggttttatgc aaggaaggta tctggaaagc
    19621 cagcgtgatg tcgaagccgc agataagccg tttgagttct ttatgaatcg cttccgtctg
    19681 ctggaggccg cgccgcgcgt ggagtttatt gcgtataccg ggctttgcga agatgtgatt
    19741 cgcccacagt tagacgaggc gattgcccag ggttatctca ccgaatgtgc ggattactgg
    19801 cagataaccg agcacggtaa attattctta aactcacttc ttgaattatt cctcgctgag
    19861 taatcattaa tgcgcctgcg ggcgcattaa tcacttcttt tccccccgtt aattaacttc
    19921 ggcaaaatat atttcttact gaataaacat cgatgatatt tattagatat atctagtgaa
    19981 tatttactca tcatttattg attataaaca tgtacaacct ggcgcacaaa tttaatattt
    20041 aatttctgac tttatttcaa gttgtgataa acatagacaa gttgtcagat aggttaacca
    20101 atttgtcgca caaagcactg ataagcacaa ttagatatca accaatttcc atccttcaac
    20161 agggagtttt tttatgaaaa tgaggtcatt tacccgctca ttagtctgcg catccttgct
    20221 ggctctggtt agcactggcg taaacgccgc agaaaaagtg acgttaaagt tagcacacaa
    20281 cctggaaaga agtcatgttg tacatcagag ttttgaagag ctggcgaaag aggtaaaaca
    20341 gctgtcagaa ggtaatatgg tgatccgtat ttatcccagc agccagatgg gtaatgcgcg
    20401 ataaactatg gaattgttgc aaaatggcgc actggatatg accaaaggtt ccgccagtga
    20461 tctggagtct tttgataata tttatgctat ctataatttg ccgttcttat ttaaagatca
    20521 ggctcacttc aacaaagttg tttttggtga agttggtaaa gaaattatgg actcaacaaa
    20581 agacaaaggc ttttttgctt tatcagctta tgtagcgggt acacgcagtt tctatgcgaa
    20641 aaaaccgatt actaagccag aagatcttaa agggctgaaa attcgtgttc agccaagtcc
    20701 gaccaccatc aaaatgattg aactgatggg tggttcaccc actccaattt catttggcga
    20761 agtttatact gcgatgcagc aaggtgtagt agatggcgca gaaaataacg tgccttcctg
    20821 ggtgcagact cgacatattg aaattgccaa agttttttct gaagatgaac acgcctcaat
    20881 tcctgacttt ttggttatct ccaccaaaac atggaataaa ttaaccccag aacagaaaca
    20941 aatcctcgaa acagcagcga agaaatccga agcttatcaa caaaaattgt gggaaaaaat
    21001 tgacgctgac acacgtgctc aggcgaaagc tatgggggg
//
LOCUS       DAJTYZ010000072        21003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000072 DAJTYZ010000000
VERSION     DAJTYZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.072"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..170)
                     /locus_tag="OO596_002491"
     CDS             complement(<1..170)
                     /locus_tag="OO596_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718326.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="OO596_002492"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="OO596_002492"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6718327.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="OO596_002493"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="OO596_002493"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6718328.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="OO596_002494"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="OO596_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6718329.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="OO596_002495"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="OO596_002495"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6718330.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="OO596_002496"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="OO596_002496"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6718331.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="OO596_002497"
     CDS             4288..5151
                     /locus_tag="OO596_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6718332.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="OO596_002498"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="OO596_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6718333.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="OO596_002499"
     CDS             6489..7106
                     /locus_tag="OO596_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6718334.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="OO596_002500"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="OO596_002500"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6718335.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="OO596_002501"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="OO596_002501"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6718336.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="OO596_002502"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="OO596_002502"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6718337.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="OO596_002503"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="OO596_002503"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6718338.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="OO596_002504"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="OO596_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6718339.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="OO596_002505"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="OO596_002505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6718340.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="OO596_002506"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="OO596_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6718341.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="OO596_002507"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="OO596_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6718342.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="OO596_002508"
     CDS             18072..19781
                     /locus_tag="OO596_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6718343.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="OO596_002509"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="OO596_002509"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6718344.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5082 a         5713 c         5386 g         4822 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcttg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAJTYZ010000073        20713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000073 DAJTYZ010000000
VERSION     DAJTYZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.073"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO596_002510"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO596_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6718345.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO596_002511"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO596_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6718346.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO596_002512"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO596_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6718347.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO596_002513"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO596_002513"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6718348.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="OO596_002514"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="OO596_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6718349.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="OO596_002515"
     CDS             complement(8214..9107)
                     /locus_tag="OO596_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6718350.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO596_002516"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO596_002516"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6718351.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO596_002517"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO596_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6718352.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="OO596_002518"
     CDS             complement(10238..11470)
                     /locus_tag="OO596_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6718353.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO596_002519"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO596_002519"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6718354.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO596_002520"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO596_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6718355.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="OO596_002521"
     CDS             complement(14275..16539)
                     /locus_tag="OO596_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCR6718356.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OO596_002522"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OO596_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6718357.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OO596_002523"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OO596_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6718358.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OO596_002524"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OO596_002524"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6718359.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="OO596_002525"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="OO596_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6718360.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4970 a         5732 c         5046 g         4965 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat gtg
//
LOCUS       DAJTYZ010000074        20396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000074 DAJTYZ010000000
VERSION     DAJTYZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.074"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..1337)
                     /gene="casA"
                     /locus_tag="OO596_002526"
                     /pseudo
     CDS             complement(1..1337)
                     /gene="casA"
                     /locus_tag="OO596_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(1435..4134)
                     /locus_tag="OO596_002527"
     CDS             complement(1435..4134)
                     /locus_tag="OO596_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCR6718361.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(4329..4481)
                     /locus_tag="OO596_002528"
     CDS             complement(4329..4481)
                     /locus_tag="OO596_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6718362.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(4746..5480)
                     /gene="cysH"
                     /locus_tag="OO596_002529"
     CDS             complement(4746..5480)
                     /gene="cysH"
                     /locus_tag="OO596_002529"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6718363.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(5555..7267)
                     /gene="cysI"
                     /locus_tag="OO596_002530"
     CDS             complement(5555..7267)
                     /gene="cysI"
                     /locus_tag="OO596_002530"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6718364.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(7267..9066)
                     /gene="cysJ"
                     /locus_tag="OO596_002531"
     CDS             complement(7267..9066)
                     /gene="cysJ"
                     /locus_tag="OO596_002531"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6718365.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            9385..9747
                     /gene="queD"
                     /locus_tag="OO596_002532"
     CDS             9385..9747
                     /gene="queD"
                     /locus_tag="OO596_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6718366.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            9825..11096
                     /locus_tag="OO596_002533"
     CDS             9825..11096
                     /locus_tag="OO596_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6718367.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            11087..11347
                     /locus_tag="OO596_002534"
     CDS             11087..11347
                     /locus_tag="OO596_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6718368.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            11364..11939
                     /locus_tag="OO596_002535"
     CDS             11364..11939
                     /locus_tag="OO596_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR6718369.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(12087..12947)
                     /locus_tag="OO596_002536"
     CDS             complement(12087..12947)
                     /locus_tag="OO596_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6718370.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(12944..13723)
                     /locus_tag="OO596_002537"
     CDS             complement(12944..13723)
                     /locus_tag="OO596_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6718371.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(13701..15110)
                     /locus_tag="OO596_002538"
     CDS             complement(13701..15110)
                     /locus_tag="OO596_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6718372.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(15132..16586)
                     /locus_tag="OO596_002539"
     CDS             complement(15132..16586)
                     /locus_tag="OO596_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6718373.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSRPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(16656..17472)
                     /locus_tag="OO596_002540"
                     /pseudo
     CDS             complement(16656..17472)
                     /locus_tag="OO596_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            17790..19067
                     /locus_tag="OO596_002541"
     CDS             17790..19067
                     /locus_tag="OO596_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6718374.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            19094..20396
                     /locus_tag="OO596_002542"
                     /pseudo
     CDS             19094..20396
                     /locus_tag="OO596_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         4892 a         5416 c         5043 g         5045 t
ORIGIN      
        1 cactaacctc aggaagcgat gctgagtttt gttccagaag tcgatatcca caaaactaaa
       61 atcacccctt gcaccttttg gatcggagaa ccatgcttct ttcaatgcac tccgtaacag
      121 acttagaatc cgtgaagccg tttgcgcagc cagccgcagt ttcggtatct ggccttcttt
      181 ttattgagca gcagcgggaa atgatgttcg taccaacagc gcgctttcat gttgtcgaaa
      241 tcatagccaa atccccacag gcccactttt gcctgcttga gactgctggc attaaagagt
      301 ttcaccacca gcgcgggaag ttccgtattg ttttctgact tacccgtttc gataaggcct
      361 aaccagtcgc gccagattaa accgcccggt tgtggtttga ccgagtaaaa ctcgccgccc
      421 tctttaagta gtacacggta aggcgttaag ggatgctgcc acatggcata gttagcgccg
      481 taatttttgg tcgtcatcag gctcagaagc gcgtcgctct gctcaccgca aatatcgcag
      541 ttgccgactg tggtggtatt aaaatcaata cgaatacggc gaggcattcc ccagtacgcc
      601 tggagtttat tgacctgctc atcggttacc accgcaccgg ccagttcgct gatgcgcgtc
      661 gggccaagcc aggggaaaac cagatcgtca aattttttgg gtagcggtaa atcggcttca
      721 tcctgcggca tgacattgat ccacagtttg cgccacaagg gagtttgttg attaccctga
      781 tattcctgta attcaatcag agtggtcatc ggcccaccgc cgcgtaaacc ggtgcggtag
      841 cctttgccac ctgacggcgc attcaactgt agggagaaca gagctaacgc agagcaatgt
      901 gggcacaggt attccgttac accacgcttg ataaagtggt ctttattaaa cttcgttgtt
      961 tgagcgccgg gaatctcagg cagtagcgaa gcgaccggaa ctttatctcc ggtgagcgcc
     1021 tcgaaatcct gtataaatga aggggaatct ggcccaaact ggaaagcatg ttctaatgac
     1081 agcaatgctt cccgtagctt ttcagcttcc agtccgtctt cccagatatc atcccaacga
     1141 cgatgatctt ttggcgcgaa gctggtttgt agtagcccca gcaaaaactg ccataccgca
     1201 ccctggagat ctgcccgcgg cgcagaaata tcgacaacat tttcatccgc cagatcgact
     1261 ggcgccagtt tgcctgtcgt tccgtcttta aaacgaacgg gcaaccacgg ggttgtcaga
     1321 agtgaaaacg agttcatacg catcctctcg taaaaaagac catcccaggt cggttgtcca
     1381 tctttccatc cttttcggtg tatcaactat tcagagagtc ctgtcgcaga catctcaacc
     1441 aaccttgcca atcaatcctt ccgtagccga atacccacaa ctttcgtcat cgttgactaa
     1501 aataacgttt gccatctccg gatcttgtcg ctgttcaatg caccacagcc tgaacgcctc
     1561 tccctccagt aaagaaaact catcccgatg ttttttccac cagcttcggc gcactctgac
     1621 aacgctcatt tcccatgcgt gagcaccggt ggcataaggc ttcaccacac cgtcaataca
     1681 ggtagccagc cacagggaaa cagactcctc agccagacgt gtcgacagtt tttctgggag
     1741 gtaatcgttg atattggcgg catagccggg cctgaagttc agcacaaact ttttagccat
     1801 tgcgcgatca cagtaatatt tgcccacttg ctcctgctcg ctgcgggcaa atccttccgg
     1861 cattaccacg tcctcaccgt agacggattc aataagaagc cgggctgcgt gtggcatctg
     1921 aatagcacct tgctcacgca gcacacgctg cgtcagccag attcgcccat gatcgggata
     1981 gacaaatgca ctgttacgca tggcactgcc aaaccattcg tcgccaggag agtcgtccca
     2041 gacgggagcc agaatcagca attcaggggg ggaacgctcg tcctttccgt cacgctttaa
     2101 ctgaccatta atatcgcgga tatgccgctg taatcgtcct gctcgctgaa tcagcaaatc
     2161 aacaggggcc aggtcggaga tcatttcgtc caggtcacaa tcgacactct gctctaatac
     2221 ctgagtacaa ataaggactt ttccggcacg ctgtgagcta tcttctttac caaagcgtgc
     2281 cagtgtctcc gtttcaattc gctggcgatc gctaaaagca aagcggctat gaaaaagtga
     2341 aaggctggaa gcaggaatga cgccgcgggc aagcagctga cgatgaacct taatagcgtc
     2401 atcgacagaa ttccggatcc aggcgatgca tttcccctga cttaccgccg attcgatacg
     2461 cgcaatacac tcttgttcac tatgaagcca acccacgctg acgctacgct caacgtcttt
     2521 gcgcgtcgct acccggtgtg agttcacatc ggatttcgtg acatgcgtca gccaggggta
     2581 atcatccttt tccaggaacg gagcttcttg caggccctct gtaccccgcg caaaggcagc
     2641 gacgagtttg tcgcgctgct gttgagataa cgtggcagaa agcaaaataa cgctgtttcc
     2701 gccacgcgcc tgccgctcga tcaacccttc aagaatgcac gacatgtaag cgtcacaggc
     2761 gtggatctca tcagccagca ggattttgtt actcagcccc agaagccgca gattattatg
     2821 tttgaacggc atcaccgcca tcatcgcctg atccagcgtg ccgacgccaa tttcagccag
     2881 caacgccttc ttgttactgt cggcaaacca ggccgcacat ccctgactga acgtttgttc
     2941 atccggttct tctgacccga ctaaatcacc agaccagagt gattcattaa agcggtccat
     3001 taatgtgcgg gcactatgtg ccagcaccag gctggggcgg gattctggcg aatagaaagc
     3061 aagccaggtt ttgaccagcc gatcgtacat ggcattggcc gttgccattg ttggcagacc
     3121 aaaaaacagc ccctgagcct tccccgcagc tatcaacctg tgcgccagga taagcgccgc
     3181 ttctgtttta cctgcgccag tcacatcttc cagaataaat aactgcggtc cttgctggct
     3241 gatatccaga tccagcacct tttgctgtaa cggtgtcgga ttctcaataa aaggaaacag
     3301 cgtattaatt ccgttgaaag gcgcgacttt tgcttttaga ggaaagacgg ttaaggcgtt
     3361 ttgagcctga actaaagttt tctgccagta acctttaata tccattgggt gtgcgacgcg
     3421 tggaaaaaat cgcgttgacg aacccgtcca gtctgcgagc acgacggttg cagagatata
     3481 ccaggaaagg tgttttataa gttcaatgcc ctcatcatca tcccagaatg cggggatctc
     3541 tatgagcgga aacagtggtt tgatttcaag gagaaaatct cgtgctgcag ctttgtcttc
     3601 aggcagaaaa ttatccaatt catccatacg atcagggggc caaccatgat gccctgtcgt
     3661 cacgggcatc cacatttcta tcactcgcct aagtttgcgc ggagagagcg aagatgaagg
     3721 aagtaactcc tgacattcac ttaaataatg attccacaac cagtagccca gcgttgaatg
     3781 agagattttt tcgtaatttt ttctggcacc ttcctgaatc ttgagttcag ggggcaggta
     3841 aagttgttga aaagagcggg caaattttcc aatatcgtgc cagcacagca accaggcgaa
     3901 aaattgagcc gcctgttcct tgtcagaaat ccctaattga cgaaaataat cagccagccc
     3961 gaagcaattt cttttaacca ttaaatagcc catcgcggct acatccagcg aatgccagca
     4021 aagaaggtga tagtcgtcgc ccccgtcttt ctcgccgcgt cgggtttttc cccagaaatc
     4081 aaaaaaagtc acaatatttt tatccttcag taaacttaaa ggatatttac gcatatatta
     4141 aaaattatct gtgatatata tcattcaata aaatatttac attatgaata atcaaataat
     4201 tttcatccgt aatatatatg aataataaac ataggcataa tattcacata aataatataa
     4261 attaaaaaag ggtgcaggag accaaaccat cacaattcag tacgcggggt tttacccccg
     4321 cgtggtggct acttcttcga ttcgtaagcg agaactgcct taaactccat accacgttct
     4381 ctgatactca actcacacaa cgagtcgcct accatcagcg tgaatcccag tactgtaaaa
     4441 cacagcacta tgattgccac aagggcatat tttgtcagca tgtcttgcct ccctgcaaag
     4501 aagagactaa cattcgaatt gtttgggttc gaacgctggc ctcaggttga tagaaatatc
     4561 gcctggggct tttgtccatc tggaacctcg cgaatgctta acgccagaca gcctcaagca
     4621 cccgacgcca ttctatacct gataattctt ccagcggcgt tcacgtttaa aatcaggaat
     4681 actgctcgca aaataaaaac gcccccatca tttctgacag aggcgtttaa tttatccggc
     4741 aatatttacc cttcgtgcaa tccacattcc ctttttaagc caaagaaacg tgtttcttct
     4801 tccgacatgc caggttccca tttacgggtt gtatgggtat caccgaccga taaatatcct
     4861 tcatcccata atgggtgata tttcaggcca tgtttttgca ggtactgata aatagttcgg
     4921 ttatcccagt cgataatcgg cagcacttta aatacgccac gctgaattgc cagcaccggt
     4981 aaattggcgc gactgccgga ttgttcacgg cgcaggccag caaaccaggt ttgcgcgtta
     5041 agttctttca gagcccggtt catcggttcg actttgttga tgtcattgta cttttcaatg
     5101 ccttcaacgc cctgctccca cagttttccg tagcgtgctt cctgccaggc tgcgctttcg
     5161 gtagcacggt acactttcag gttgagcttg agtttgtccg ttaactcgtc aataaagcgg
     5221 taggtttccg ggaacaagta acccgtatcg gtgaggatca ccggaatatc cgggcgaatt
     5281 tgattcacca gatgcaggct caccgccgcc tgaatgccaa agctggaaga aagtacatat
     5341 tcaccgggca gattatccag cgcccaggct acgcggcctt cagcgtccag tttttccagt
     5401 tcggcgttag tttccgccag cgccagaata cgatctacct tcggcagttc gttcagggcg
     5461 tttagatcga gtttggacat agattcctca ctgtttgcct tgcctgatgc gacgctcacg
     5521 cgttcttatc aggcctacaa gaccgggctg atggttaatc ccacaaatcg cgcgccggat
     5581 cgagcaccgg gcgaatgatg cccgcacgca ccgtaaagtc gccgaagcct tcacccgctt
     5641 cgcgctcttt cgcccagcgc cctatcagtt catcaagcga cgccaggatt tccggctcgg
     5701 tgatgttttc tttatacatc cgtgggatac gtgtcccaat gcggttgccg ccaagatgca
     5761 ggttgtagcg acccggcgct ttacccacca ggcccacttc cgccagcatc gcgcgaccac
     5821 aaccgttcgg gcagcctgtt acacgcatca cgatatgctc atcgctgaca ccatgtttcg
     5881 ccattaaatt atcgatgttg tcgataaaag acggcaggaa acgctctgct tccgccatcg
     5941 ccagcgggca agtcgggaat gacacgcagg ccatcgagtt ttcacgctgc ggcgtgacgg
     6001 cattcattaa cccgctctct ttggcgatct tctcgatctt cgctttctcg ctttccggta
     6061 caccggtgat gatcagattc tggttcgccg taatgcggaa atcgcctttg tggatcttcg
     6121 cgatctccag caggccggtt ttcagcggac gccccggata atcaaggatg cgaccatttt
     6181 cgataaacag cgtcaggtgc cagttatcat caatgccctt aacccagcca atacgatcgc
     6241 ctcgtccggt gaactcatat ggacggatcg gttcaaattt gatccccgcg cgacgctcca
     6301 cttccgcttt aaacgtctca acccccacgc gctccagcgt gtatttggtt ttggcatttt
     6361 tacgatcggt tcggttaccc cagtcacgct gagttgtcac gacggcttcg gccaccgcca
     6421 gcgtatgctc cagcggcaga tagccaaact cgctcgccgt gcgggcgtag gttttcttgt
     6481 tgccgtgttc gatagaaagc ccaccgccca ccagcaggtt gaagcctacc agcttgccgt
     6541 tttcggcgat tgccacgaag ttcatgtcgt tggcatgcag atcgatatcg ttctgcggcg
     6601 ggatcactac cgtggttttg aacttacgcg gcaggtaggt ctgaccaagg atcggttctt
     6661 catcagtagt ggcgactttt tcctggtcga gccagatctc cgcatacgcg cgggtacgag
     6721 gcaacagatg ctcagaaatc ttcttcgccc actcgtacgc ttccgcgtgc agctgcgact
     6781 cgtaagggtt cgaggtgcag agtacgttac ggttcatgtc gttagctgtc gccagcgcat
     6841 caagaccgac cgagtgcagc atctggtgca ccggtttgac gttctttttc agaatgccgt
     6901 ggaactgaaa cgtctggcgg ttggtcaggc gaatgctgcc atagatggtg ttttcaccgg
     6961 caaatttgtc gatcgcctgc cactgtttag tggtaatcac cccacccggc agacgacagc
     7021 gaagcagcat cgcgtggcgc ggctccagct tctgttcagc acgttcggca cggatgtcgc
     7081 ggtcatcctg ctgatacatg ccgtggaagc ggatcagcag gaagttgtcg cccttaaagc
     7141 cgccggtcag accgtcgttt aaatcttccg caatggtgcc gcgcaggtag ttgctttcaa
     7201 gcttcatgcg ctcggcgtct gtcagttttc cttcgaccac taaaggccct ggatgttttt
     7261 cgctcattag tagacatctc gctgataacg gcgctctacg cgcagctcac ttaaaaattc
     7321 atccgccgct tcggtgtcca tgccaccaaa ttcggcaatc acttccagaa gtgcctgctc
     7381 aacgtctttc gccatgcgat tagcgtcgcc gcagacataa atgtgggcac cgtcattgat
     7441 ccagcgccac agctccgcgc cctgttcgcg cagtttgtct tgtacataaa ctttttcttt
     7501 ttggtcgcgc gaccaggcaa gatcgatacg cgtcagcacg ccctctttga cgtagcgctg
     7561 ccactccacc tggtagagga aatcttccgt aaagtgcggg ttgccaaaga acagccagtt
     7621 tttacccggc gcttcgtcgg cggcgcgttg ctgcataaag gcacggaacg gcgcgatgcc
     7681 ggtaccgggg ccaatcataa tcactggcgt ttgcgggtta gcgggcaggc ggaaattgtc
     7741 gttatgttcg ataaatacgc ggacttcgcc ctcttcttcc acgcgatccg cgaggaagct
     7801 ggaggcacca ccggcacggg cgcggccttc cacgtcgtaa cgcaccacac caacggtgac
     7861 gtgtacttcg ttctcgactt ccgcctgcga ggaggcgatg gaatacaggc gcggggtcag
     7921 cgggcgcagc aggttaatca gcgcttcggc atccagttgc gccggagaga aacgcaccat
     7981 gtcgacaatc ggcgtggtcg cggcgtaatg ctgtaacttc gctttatcgc ccaccagcgg
     8041 cagcagcgtt tcgctgcggg taagcgtggc atagttctca acaatgttgg cggtgttgac
     8101 ggtcagttcg aagtgccact gtagcgcttc gttcagaggc aacgttttgc cctcgacggt
     8161 gacaggttca tcgcctttca gccacagcag ttcgacaagt tctttcacca gtgccgggtc
     8221 gttctgatac cagacaccca gcgcgtcacc cggctggtaa cgcaggcctg aatcgcctaa
     8281 gtcaatttcg atatgacgaa cgtctttttc agagttacgc ccggtaattt tctggttaac
     8341 cgaaaggctc gccaccagcg gcgcgtcttt gctgtacggg ctggtgtgga tttcatttac
     8401 cgcgccagta gcgacggatt gcgaaggtgc cgcgacaggc gcacgcgatt taagcgcatc
     8461 aaccacgcgg gcgcgccact cgctggcagc agcctggtat tcaacatcgg catcgacacg
     8521 gtcgagcagg cgttcaccac ccagttccgc cagcttgctg tcgaaatctt tcccggactg
     8581 gcagaaaaat tcataagagc tatcgccgag gctaaacacg gcaaacgcgg tgttttccag
     8641 ctttggcgct tttttggaga acaggaactt atgcagcgcg acggcttctt ccggcggttc
     8701 cccttcccct tgcgttgacg tcactacgat gagcagtttt tcgctggcga tttgtttgaa
     8761 tttatagtcg cccgcgttca ccagcttaac gttcagtttt gctgctaata aatcatcgcg
     8821 taatgcttca gcaacccggc gcgcattgcc ggtttgcgag gcggagataa tagttatacc
     8881 cggcatttct gcggctggcg ctggcgtcgc tgcaagcgca gcaggctgct gattgagtac
     8941 gccccagaaa tagccagaaa cccaggcaag ctgggtaggc gttaaatcgg tcgtggccgc
     9001 ctgaaggcgt gccagttgct ccgggttcaa cggaagcaac gcggaaggtg ggacctgtgt
     9061 cgtcatgcgt cgttatgttc cagtaagcaa agctgtttct gcgccctgtc agcgcccata
     9121 aaacagaaga gaagataagg ttaacggggt gaacggtgtg gattaaagac gggatagcga
     9181 taactaataa ccaaatcgac taacctgttt tagcaatagt ctttaacaac aaaatagatt
     9241 aaccaaccca atgaaaaaca aatgaattta gccaatcatt aagataaatc agcgattttg
     9301 cgcaacaaag tcgttttaga taatgcgaaa aaacagcctt tccggtactc tacggcggtt
     9361 ttatcgtcct gtagagaaat tatgatgtcc accacgttat ttaaagattt caccttcgaa
     9421 gccgctcacc gcttaccaca cgtcccggaa gggcataaat gtggtcgcct gcacgggcat
     9481 tcctttatgg tgcgactgga aattaccggg gaagtcgatc cgcatacggg ctggattatc
     9541 gatttcgctg aactgaaagc ggcgtttaag ccaacttatg agcgcctcga tcaccattat
     9601 ctcaatgata ttccaggtct ggaaaaccca accagcgaag ttttagcaaa atggatttgg
     9661 gatcaggtta aacccgttgt gccgctgtta agtgcggtga tggtaaaaga aacctgcacc
     9721 gcagggtgta tctatcgcgg cgaatgataa gagtgtgtcg gcggtcaatt tcccttaagt
     9781 aacgctatgt tagggtgttg tgttctggat atctggggca tgacatggaa gacgactgcg
     9841 acattattat tattggtgcc ggtattgcag gcaccgcttg cgcgttacgc tgcgcgcgag
     9901 cgggtttatc cgttttgtta ctggaacgcg ctgaaatccc cggcagcaaa aatctttccg
     9961 gcgggcggtt atatacccat gcactcgcgg aactcctccc gcaatttcat ctgaccgcgc
    10021 ctcttgaacg acgcatcact cacgaaagcc tttccctgtt aacgcccgat ggcgtaacga
    10081 cgttttccag cttacagccc ggcggtgaat cctggagtgt attacgtgca cggttcgatc
    10141 cgtggctggt tgccgaagcc gaaaaagaag gtgtcgaatg catccccgga gcgacggtgg
    10201 atgcactgta tgaagaaaac ggcagagtct gtggcgttat ttgtggtgac gatattctcc
    10261 gcgcccgtta tgtggtgctg gcagaaggtg ccaacagcgt cctggctgaa cgtcacgggt
    10321 tagtgactcg tcctgctggc gaagcgatgg cgttggggat caaagaagtg ctgtcgctgg
    10381 aaacatccgc tattgaagaa cgttttcatc tggagaataa cgaaggcgca gcgttgctgt
    10441 tcagcggcgg aatctgtgat gacttacccg gcggcgcatt tctttatact aatcaacaaa
    10501 cgctctcgtt agggattgtt tgcccgctct cttcccttac gcaaagtcgt gttccggcaa
    10561 gcgagctgct gactcgcttt aaagcgcatc cggcagtgcg cccgcttatc aaaaacacgg
    10621 aatcactgga gtatggtgcg catctggagc cagaaggtgg cttgcacagt atgccggtgc
    10681 aatacgccgg taacggctgg ctgctggtgg gcgatgcgtt gcgcagttgc gtcaataccg
    10741 gaatttccgt gcgcggcatg gatatggcgc tgactggcgc acaggcggcg gcacaaacgc
    10801 taataagcgc ctgccagcac cgcgagccgc aaaatctgtt tgcgctttat catcacaacg
    10861 tagagcgcag cctgctatgg gatgttctac aacgttatca gcatgttccg gcgcttttgc
    10921 aacgccctgg ctggtatcgg gcgtggcctg cgttaatgca ggatatttcc cgcgatttat
    10981 gggatcaggg tgataaacct gttccaccgc tgcgccagtt attctggcgt catttacgtc
    11041 gtcatggcct gtggcatctg gcgggcgatg ttatcaggag tctgcgatgt ctgtagccca
    11101 taatctctgg cgcgttgctg atgcgccgca cattgttccg gctgactccg ttgagcgtca
    11161 gatggtacaa cggttgatta acgcctgtcc ggcaggtctt ttttcgctca caccggaagg
    11221 tgacttacgc gttgattatc acggctgcct ggagtgtggc acctgccgtt tgctgtgcga
    11281 cgaatccaca ctacaacagt ggcgctatcc gccttccgga ttcggcatca cctaccgctt
    11341 tggataaata ataaggataa tttatgcccc tcttacacct actccgccag aatccggtga
    11401 ttgctgccgt taaagataat gccagcctgc aactggctat cgattctgaa tgccagttta
    11461 tttccgtgtt gtacggcaat atctgcacca tcagtaatat cgttaaaaag attaagaacg
    11521 ccgggaaata tgcttttatt catgttgatt tgctggaagg cgcgtcaaat aaagaagtcg
    11581 ttattcagtt tttgaaactg gtgaccgagg cggacggcat tatcagtacc aaagcctcaa
    11641 tgttgaaagc ggcaagagcg gaaggttttt tctgtattca tcgcctgttt attgttgatt
    11701 caatttcgtt tcacaacatt gataagcaag ttgcgcaatc gaatccggat tgtattgaga
    11761 tcctgccagg cggtatgccc aaagtgctgg gctgggtgac agagaaaatc cgccaaccgc
    11821 tgattgccgg tgggctggtg tgcgatgaag aagatgcgcg taatgcgatt aacgcgggtg
    11881 tcgtggcgct ttccaccacg aataccgggg tctggacgtt agcgaaaaaa ttactttgac
    11941 gggataatcg taaccaattg aatttggttt gatttgtagg ccgcacgcca catccgacat
    12001 tcagcgcctg atgcgacgct tgacgcgtct tatcaggcct acaagtcccc aggccagaac
    12061 cgtaggcagg atcatccggc aacacgttat tggcaatctg cgtgaatgtt tgtcaccagt
    12121 gcctccagca ccactttcca gtcatcaacc acgccaacat ctgcctgtga aaataccgca
    12181 gcgctggcgt catgattaat cgccaccaca aacttgctat tgcgtacgcc cgccattaac
    12241 gctgctgcgc cagaagcgcc aacaacaata cacacctcag gtgccagcag atgtccggaa
    12301 ataccaatca ctttttcagc atcaacgcca ccgttcatca ctcgcgcccg actgtagccc
    12361 acttcggctc ccagcttttc tgccagcata gcgatttcct ggttatctgc ctctccgccc
    12421 tgccccacca ccagcacccg tctggcctca gctaatgggt cgcgagtaac atttttcaga
    12481 tcctctgtgc taaccagcca gtccggaagc gcacccggga caatgttcag ttgttgcatt
    12541 ccagagggca acgtggcgtt tttcgctgct cccgcctgac gcgccaggga aaggcatagc
    12601 gggcgttttt ctgtttgcag cgtcgccgtt agcgcatttc cccagtggga tttgcgcacg
    12661 cttacggttg gaatatctaa cgaagtcacc tggcagatgc ttgcaccatg taaacgccag
    12721 gccagccggg tcgacagttc atcgccaaac gttcccggcg gaaagagaac aacgtccgca
    12781 ggcgttcgct gccactgttc caccaacgca tccagcacct gttccgccac tacaggctga
    12841 ggttcaatct gccagtgcgc cagggtacaa ccgctgaaat cctgcgccgc cagccagctt
    12901 gctattgcgg cattctcctg atttattgtc acaattgcga tattcatggc tgcatccttt
    12961 ggcgtaaata gtcctgccat agcttttgag ctttttctgc taccgtttgc ccatcaatca
    13021 gcgtcgctcc gcgtcgttgc tctgctcttg ccagttgcag acactgcatc gctggcattt
    13081 cggcagcaac agttttacgg ataatttctg ctttccccgc agccattcgc tggcgcattc
    13141 ccggtactgg caaggccact tcaccgcact gacgcacggc aatcaccgca ggcaagcgaa
    13201 cccggcaaca acgcaaccca tgttcagtac gctgttcgag ggtgataaac agcgcgtcga
    13261 gagtgaaacg ctccacctgg gtgaagcagg gccagcccag catttccgcc agcaaaaatg
    13321 gcgtttgccc attctgccct tcgctgcttt ggcagccggt aattatcaga tccagcggat
    13381 tctgatgctg ccattcggca atatggcgag cgacaaattc cggcgcaaag cgcagatctg
    13441 ccgccgtctc cagcaataca gcttcctcaa acccaagagc catgagatag cgcagccagt
    13501 gcaatgcccg ttcatccccc atgctcaacg cggttaaaga catcggcgta ccgttttttc
    13561 tctgcgccaa cagcagcgcg gcagcggcct gttcatcagc accgagtaaa cttcgcagta
    13621 gcgaaatatc cggtccgctt ttaccctgag ccgccgcctg ccactctttt tccgccagca
    13681 ttccggcatc cggttcggct ttaaacgcta acagaatgtt cattcgcacc tcctacattt
    13741 cccgccgcca ccagcgggag ggctttagtt tctggtgccc atagccaggt cacaacaaaa
    13801 tcaaccaaca acactgtcgc caggagcaat aaggtgactt gcattcccca ctgcgccagc
    13861 acccacggca gcaggccagt acttaccgcc gcgccaagtc gactcatggc ggtggcaaaa
    13921 ccgacgccca gcgagcgaat gtcagtagga aaactttccg caggcaaaat gcccaccaga
    13981 ttactgactg ccgaaatggt ggtgctgaag agaacaaaaa gcagcagcgt taatgaactg
    14041 ccggaaggca aacaggccat cactaccagc gttgccgcca gcagcaaaaa acttcccagc
    14101 aaaaatttgc gatgtgccag caggtgcgtc agaactaatc ccagcagcgc gcccacaatt
    14161 aacaacgcat taagcatcag gctggcagtc agcgcatctt ccagaccaat agtctgggcg
    14221 atagttggca gccaggtata aatcacaaac catgggatta cgaggcagac aaagaagacg
    14281 ctgttaaacg ccgtgcgccg ccagtaacgc gaagagaaca aggttttgat gtgtttatgg
    14341 gtcgccgtta ccacttcatc gcccagtaaa acatggggac caaaatagcg atgcacgata
    14401 gcgtgagctt ctgcaaaacg cccctggcgc agtagccagc gtggtgattc tggcgttccc
    14461 cagcgtaata acgtaatcaa caacgcgggc agagctgccg atgccagtag ccagcgccag
    14521 gcctccgggt tttcggaaat aaagtgatgt ccggcaatac ttgccagcac atagcctacg
    14581 gtccacacca cgctgaatgc gcccagcaaa ataccgcgat ggcggcgcgg ggaaaattca
    14641 gccagcaagg tgtgacctac tgaataatcg cctcccagac caatgccaat caaaatgcgc
    14701 agtccaataa gatgctctgg cgtggtggca aaaaattgta aaaacgaagc aagcgtaatc
    14761 agcaaaaagc tgaaggtgaa gattttttgc cgaccaatat ggtcggagat ccacccaaga
    14821 accaggctgc caaggaacaa accaaggagc gccgagccgc cgatcattcc cgccataaac
    14881 ggcgtcagtt gcatggcggg cgtaagctga ataatggcgt aaccaatgac gccgagaaca
    14941 taaccgtcgg tcaggtgtgc gccgaaagtg agcgcagcaa tgcggcagtg aaaacggtta
    15001 agcggtaaat catccattcg caccggtgaa gtgttcatct ttaccccttc attgtccggt
    15061 aataagaacg gcttctcgcc tgagaagccg aaaaggcaac gccccgtcat cgcggaacgc
    15121 tgcctgacac attatttttc aatcggatag atagtacccg tattcataat gccattagga
    15181 tcgaactgct ttttcagccc ttccagcaac gcccacgcgc tgccgtgttc cagtttgctc
    15241 cagtgaacgc gatgtttacc gataccgtgg tggtgcacca tcgaaccgcc gaggcgaatg
    15301 gtttcttcac agatgatctt gttgagcgga ttgtggtact tgtcgatttc ctcttccggc
    15361 ttacagtcaa cgacgttgta atcgtagacg aagtacatgt tggtgccgtt ctgatagcta
    15421 tgagaggaat gaccgcccag catggtgatg tcgtcggcgt gcgggaactc agtacgaata
    15481 cggttaataa cgctttcgta gatttcgtgg atgcagctcc agcagccgga cacttcagtg
    15541 gtaaagccca tgttgccggt tttgaggatc tgcacacgtt cggcagccac tttatccggt
    15601 ccccagttca ggttgttaaa ccaggtttcg atcagcttgc tgtccacgcg ctggcattgc
    15661 gggtagcggg caacgatttc cgcaatccct tcgcccgtcg ccttcgcaat gcgagggtta
    15721 ccttcagcca taaagatcag cacgcatttt ccgtctgcaa aatgggtgaa gtgttgggtg
    15781 ccatcttcag cgtcatacaa acgagcgatc gacggacgat acccttccac catgacttca
    15841 cgcaggatgt tgaagtcggt tttcatgtct tccaggatat agccgtagaa gaggttgttt
    15901 tccggggtga atttaaagat tttcactgtt acttcagtga tatagcaaag tgcaccttcg
    15961 ttaccgatga tgatgtggcg aatgtccggg ccagccgcgc ggcgtggcac gtttttaatg
    16021 cgtgtgacgg tgccatcagc caatactgct tccagaccaa cgaccatatc ttcgattgcg
    16081 ccgtagagtg tggagaactg cccgatactg cgggttgcta ccaagccgcc catctgcgcc
    16141 agcggccttg actgcggaga atgccccgtg gtgtaacctt tttcacgcaa cgcattttcc
    16201 agcacctcca gcggaacgcc acattgcgcc gtcgcctgca tattctcaat atcaatatta
    16261 atgatttgat tcatggcgga gccgtcaagc accaccgagt tttccacaac ggtttccagc
    16321 ccaccttcag tggcggaagc gccggtacgc ggcacaccgt taattttgtg cgcattcata
    16381 aaattcagca cacgggatac ttgctctgtg gaaccgagtt ttacgaccgc tgccggaatc
    16441 ggcaaagtat aaatgccatg aatatccgga aatttacgaa aacggtcaat actgttcttc
    16501 tttaatactg tttcatcggt aattacgcga tctgcaccaa caatttcctt tagttggtcg
    16561 acaatcgctg cgcgagataa agacatagta attccttcct gataataaaa atggaataat
    16621 cattaataag agaaaacaga aaccaccgtt atggattagc gcactaaata accaccattg
    16681 accactaata aatgcccatt gacataattt gaagccggac ttgcgaggaa tacagctgcg
    16741 cccattaaat cctgagtatc gccccaacgg tttgccggaa tatgatcaag aacgcgctga
    16801 ttggtttctg gattactgcg tgtcgccagc gtaatatctg ttgcataata gccaggagca
    16861 ataccattta cctgaatatt atattgacct agttcatcac aataagcttt ggtgaatccg
    16921 gcaagagcat gtttagtggc agaatatgca ggtgaccatt gtccacctaa gtaagagaac
    16981 aatgaacaga tattaataat tttgccgctt ttctgcggga tcataatttt tgcagcttca
    17041 tagcttaact cgaatgcggc ggtcaggttc acatcaatca tcggatccca gtcggcacga
    17101 ccgaagtcca gcaccttatt cagcttacaa ataccggcat tgtttaccag aatatcaact
    17161 gtaccgaaac gctcacagca agcagcgata atcttctgcg gcgcgccttc tgcggtgata
    17221 tccacctgca tgaagtccac ctcaacaccc tgtttttcaa tcatttcctt tgtttcgccg
    17281 ttatctttca atcatttcct ttgtttcgcc gttatctttg acgaaactag gaataaagat
    17341 atttgcgcca gctttggcca acgccatggc aaatgcctgg cctaaaccgc tattcccacc
    17401 ggtaacaatt gcggttttac ctttcagtga gaaaaaatcc attgagaacg cattgagaga
    17461 ttcgattgac atagttagct ccaatttccg taaggcaaaa aaaagaaagg taacttcccc
    17521 cgcgagaggg aagttacctt tctcattatc tctggtaact ggagataatt tttagcataa
    17581 cggcataact gctaatgtga tcgtaatcac agtgtgatat tcgtattgtc aaatagtttt
    17641 aaaaagcaga agcgtgatta ctctcacaca acacctctgc tttccatgtt atttcttatt
    17701 gcaattacta gagacctgag aaaagtaatc cacctgaaag ggcggcttac ttttctcttt
    17761 tttttgtcta ttacaaatgg gaatacgata tgcaacacaa ctcatatcgc cgttggataa
    17821 ccctcgcgat aattagtttt agcggcggcg ttagttttga cctggcttat ttacgttata
    17881 tttttcaaat tcccatggcg aaatttatgg gattcagcaa taccgagata ggtttaataa
    17941 tgagtacctt tggtattgcg gctattattc tttatgcccc cagcggcgtt attgccgata
    18001 aattttcaca ccgcaaaatg attacttccg cgatgatcat taccggatta ctgggtctgt
    18061 taatggcaac gtatccaccg ctgtgggtaa tgctctgtat tcaggtcgcc tttgcgataa
    18121 cgacgatttt aatgctgtgg tcggtgtcga ttaaagccgc ctcgttgctg ggcgatcata
    18181 gtgagcaagg gaaaattatg ggctggatgg aagggctgcg cggcgtcggt gtaatgtcgc
    18241 tggcggtgtt taccatgtgg gtcttttctc gctttgcacc ggatgacagc gccagcctga
    18301 aaacagtcat tatcatctac agtgtggttt acatcttgtt ggggattctg tgctggtttt
    18361 ttgttagcga taacaacaac ctgcgcagtg ccaataacga agaaaaacag tcattccagc
    18421 ttagcgacat ccttgccgtt ttgcgtatca gcaccacctg gtattgcagc atggtgattt
    18481 ttggtgtctt cacaatctac gccattctga gttactccac caactatctg accgaaatgt
    18541 acggcatgtc gctggtggca gcgagctaca tggggattgt gatcaacaaa atcttccgcg
    18601 cgctgtgcgg cccacttgga ggcattatca ccacctacag taaagtgaaa tcccctaccc
    18661 gcgtgattca aatcctttcc atcatcggtt tgctggcgtt gacagccctg ctcgtcacga
    18721 actctaaccc ccaatcggtc gcgatgggga ttggcctgat tttactgctg ggattcacct
    18781 gttacgcctc acgcgggctg tactgggcct gccccggcga agcgagaaca ccgtcttaca
    18841 ttatgggcac cacggtaggt atttgttcgg tgattggatt cctgccggat gtcttcgttt
    18901 acccgattat cggccactgg caagacaccc tgcccgctgc agaagcctac cgcaatatgt
    18961 ggctgatggg catggcggcg ctcggcatgg tgattgtctt tacctttttg ctgttccaaa
    19021 aaattcgtac tgctgatagc gcccccgcaa tagctagcag caagtaagcc aggaaaatta
    19081 caggagaaaa atcatgtcga aaaaatacat catagggatt gatggcggaa gtcagagcac
    19141 aaaagtggtg atgtacgatc tggaaggtaa tgtggtttgc gaaggtaaag gcttattaca
    19201 gccgatgcac acgcctgatg ccgatactgc agaacatcct gacgacgatt tatgggcatc
    19261 attatgtttt gcaggtcacg atttgatgag ccagtttgcc gggaataaag aagatattgt
    19321 tggcatcggc ctgggctcca tccgttgctg ccgtgcgtta ttgaaagccg acggcacgcc
    19381 agctgcgccg ttgattagct ggcaggatgc acgcgttaca cgcccttacg aacatacgaa
    19441 tcctgacgtg gcgtatgtaa cctctttttc gggttatctg acgcatcgct taaccggcga
    19501 atttaaagat aatatcgcca actattttgg tcagtggccg gtggattata agacctgggc
    19561 atggagcgaa gatgccgcag tcatggagaa gtttaatatc ccccgtcata tgctgtttga
    19621 tgtgcaaatg ccaggcactg ttcttgggca tatcacacct caagccgcgc tggcgacgca
    19681 tttcccggca ggactgccgg ttgtttgtac caccagtgat aaaccggtag aagctctggg
    19741 agccggatta ctggatgatg aaactgcggt aatttctctt ggcacttaca tcgcattgat
    19801 gatgaacggc aaagccttgc cgaaagatcc ggtggcgtac tggccgatta tgtcttctat
    19861 tccgcaaaca ttgctgtatg aaggttacgg tattcgcaaa ggcatgtgga cggtgagctg
    19921 gctgcgcgac atgttaggcg agtcgttaat tcaggatgcc agggcgcagg atctttcacc
    19981 ggaagattta ctcaacaaga aagcctctag tgtgccacct ggctgtaatg ggctgatgac
    20041 ggtgctggac tggctgacca atccgtggga accgtacaaa cgcgggatta tgatcggctt
    20101 tgattccagc atggattacg catggatata tcgttcgata ttggaaagcg tggcgctgac
    20161 gctgaagaac aattacgaca atatgtgtaa tgaaatgaat cactttgcga aacatgtaat
    20221 cattactggc ggcggttcga acaggatctg tttatgcaga tttttgccga cgtgttcaac
    20281 cttccggcac gccgtaacgc cattaacggt tgtgcaagcc tgggggcagc gattaataca
    20341 ggggtaggtc tggggctata cccggattac gcaacggctg tcgataaaat ggttcg
//
LOCUS       DAJTYZ010000075        20232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000075 DAJTYZ010000000
VERSION     DAJTYZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.075"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            448..876
                     /gene="uspC"
                     /locus_tag="OO596_002543"
     CDS             448..876
                     /gene="uspC"
                     /locus_tag="OO596_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR6718375.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(883..2307)
                     /gene="otsA"
                     /locus_tag="OO596_002544"
     CDS             complement(883..2307)
                     /gene="otsA"
                     /locus_tag="OO596_002544"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCR6718376.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2282..3082)
                     /gene="otsB"
                     /locus_tag="OO596_002545"
     CDS             complement(2282..3082)
                     /gene="otsB"
                     /locus_tag="OO596_002545"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6718377.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3249..4235)
                     /gene="araH"
                     /locus_tag="OO596_002546"
     CDS             complement(3249..4235)
                     /gene="araH"
                     /locus_tag="OO596_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6718378.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRIV"
     gene            complement(4250..5764)
                     /gene="araG"
                     /locus_tag="OO596_002547"
     CDS             complement(4250..5764)
                     /gene="araG"
                     /locus_tag="OO596_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6718379.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5834..6823)
                     /gene="araF"
                     /locus_tag="OO596_002548"
     CDS             complement(5834..6823)
                     /gene="araF"
                     /locus_tag="OO596_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR6718380.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7620..8123
                     /locus_tag="OO596_002549"
     CDS             7620..8123
                     /locus_tag="OO596_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6718381.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8202..8453)
                     /locus_tag="OO596_002550"
     CDS             complement(8202..8453)
                     /locus_tag="OO596_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6718382.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8568..8654)
                     /gene="azuC"
                     /locus_tag="OO596_002551"
     CDS             complement(8568..8654)
                     /gene="azuC"
                     /locus_tag="OO596_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6718383.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8917..9240
                     /locus_tag="OO596_002552"
     CDS             8917..9240
                     /locus_tag="OO596_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6718384.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9411..9908
                     /gene="ftnA"
                     /locus_tag="OO596_002553"
     CDS             9411..9908
                     /gene="ftnA"
                     /locus_tag="OO596_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6718385.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(9946..10185)
                     /locus_tag="OO596_002554"
     CDS             complement(9946..10185)
                     /locus_tag="OO596_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6718386.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            10376..11587
                     /gene="tyrP"
                     /locus_tag="OO596_002555"
     CDS             10376..11587
                     /gene="tyrP"
                     /locus_tag="OO596_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6718387.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11649..12314)
                     /gene="yecA"
                     /locus_tag="OO596_002556"
     CDS             complement(11649..12314)
                     /gene="yecA"
                     /locus_tag="OO596_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6718388.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12510..12596)
                     /locus_tag="OO596_002557"
     tRNA            complement(12510..12596)
                     /locus_tag="OO596_002557"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12560..12562),aa:Leu,seq:taa)
     gene            complement(12609..12682)
                     /locus_tag="OO596_002558"
     tRNA            complement(12609..12682)
                     /locus_tag="OO596_002558"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12648..12650),aa:Cys,seq:gca)
     gene            complement(12737..12812)
                     /locus_tag="OO596_002559"
     tRNA            complement(12737..12812)
                     /locus_tag="OO596_002559"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12777..12779),aa:Gly,seq:gcc)
     gene            complement(12964..13512)
                     /gene="pgsA"
                     /locus_tag="OO596_002560"
     CDS             complement(12964..13512)
                     /gene="pgsA"
                     /locus_tag="OO596_002560"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6718389.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13569..15401)
                     /gene="uvrC"
                     /locus_tag="OO596_002561"
     CDS             complement(13569..15401)
                     /gene="uvrC"
                     /locus_tag="OO596_002561"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6718390.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15398..16054)
                     /gene="uvrY"
                     /locus_tag="OO596_002562"
     CDS             complement(15398..16054)
                     /gene="uvrY"
                     /locus_tag="OO596_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6718391.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16350..16526
                     /locus_tag="OO596_002563"
     CDS             16350..16526
                     /locus_tag="OO596_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718392.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16513..16737
                     /locus_tag="OO596_002564"
     CDS             16513..16737
                     /locus_tag="OO596_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6718393.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(16804..17526)
                     /gene="sdiA"
                     /locus_tag="OO596_002565"
     CDS             complement(16804..17526)
                     /gene="sdiA"
                     /locus_tag="OO596_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR6718394.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(17756..18508)
                     /gene="yecC"
                     /locus_tag="OO596_002566"
     CDS             complement(17756..18508)
                     /gene="yecC"
                     /locus_tag="OO596_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6718395.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(18505..19173)
                     /gene="tcyL"
                     /locus_tag="OO596_002567"
     CDS             complement(18505..19173)
                     /gene="tcyL"
                     /locus_tag="OO596_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6718396.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19188..20174)
                     /gene="dcyD"
                     /locus_tag="OO596_002568"
     CDS             complement(19188..20174)
                     /gene="dcyD"
                     /locus_tag="OO596_002568"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6718397.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         5154 a         5165 c         4669 g         5244 t
ORIGIN      
        1 atttcaatat gttaaaaagt ttttcgctac gctagcaagc aaaaatgaaa caggaataat
       61 cgaaatggga tgttgcgcac agttaaaata aatcaccgta aataatcatc tgctataaat
      121 aatcactttc atgcaatacc agataagcta tttttaaaca gacacttacc acacaacaaa
      181 ctaattaaat acaaagaaag gcaacaatca acttaatgtt aatgaaatgt tttacataca
      241 ttaaccactt gtttaatgcg gagaatcagt acactatttt cttataatta catttgaaat
      301 tatatgatca ccgggagtga ttgattaatt gtctttacta ataatcagac taatatttac
      361 ctgtttgacc gagttgggat tgcgtcgttt ctccattagg agtaaagctt taatgtcacc
      421 tgaagttcac agaataagga acaggttatg agctatagca atattcttgt cgctgttgcg
      481 gttacaccgg aaagtcagca actgctggca aaagcggtat ctatcgccag gccagtaaag
      541 ggacacatca gtttaattac tctcgcttcc gacccggaaa tgtacaatca attagccgcg
      601 ccgatgcttg aagatttacg cagcgtgatg caagaagaaa cgcaaagctt tcttgataag
      661 ttcattcagg atgcggggta tcccgttgac aagacgttta ttgcctacgg tgagttaagc
      721 gaacatattc tggaagtatg tcacaaacat catttcgatt tggtgatttg cggtaatcac
      781 aatcacagtt tcttttcgcg agcatcctgc tcggcgaaaa gagtgattgc ctcaagtgag
      841 gtcgatgtgc tgttagttcc acttacggga gattaaccgc tcctacgcaa gctttggaaa
      901 ggtagcaact ttatcgcgct gctggctttc cgcgcttcgc ggaactatct gctttaggtc
      961 gctaatgaag cactcctgcc agtggttaat atcgtttttc acgataacgt ccagcatttc
     1021 tgcatgacgg gaaatacgtt ccgccagcga catagtcaat gcacgatcca gcgcagctgc
     1081 aacttcgtca cgatcgtagg ggttaacaat taacgccgac gttaactcgt ttgccgctcc
     1141 cgcaaattgc gaaagaacaa gaacgcccgg gttggctggg tcctgagcag caacatactc
     1201 ttttgctacc aggttcatcc cgtcacgcag tggcgtcact aagcccacgt cagagtagcg
     1261 gaatattttc atcagtaatt tacggtcaaa atgctgattc aaataataaa gcggcgtcca
     1321 gcctaattgc ccgtatttac cattaattcg tccagcttca ttttcgagct gatgacgaat
     1381 atcctgatag gcttgcacat caccacgcga cgttggtgca atctgggtat aacgaatttt
     1441 accatgatgc tgcggatatt tttccagcaa cgcttcatag gcgagaaaac gctctggcaa
     1501 acctttggaa taatccagcc gttcgacaga aaagatattt tgtacgtttt tcagttccgc
     1561 tttaagttgc gccagttttg gcggcagtgg cccggcagcc tgtttggcta tttctttcgg
     1621 ttcaatgccg atcgggtaga cttctgttcg aaatgctttg ccccaggctg tatggctttt
     1681 tgcgctacgt gtcgtgacgc gggtcaggtt agaaagacaa tccaggaacg ccagacgatc
     1741 gttttctgtc tggaaaccca gcaaatcata atcacaaagc tgttcaagca aggtgtcata
     1801 tgtcggcagc gcgttgaaga tttccggtgt cgggaaagga atatgcagaa agaaaccaat
     1861 gcgattattc actccccgtt tgcgtaattc atgcgcaaat ggcaacaggt gataatcgtg
     1921 gatccagata atgtcatcgt cttgcaacag cggcagtaat ttatctgcca gcaacgcatt
     1981 tacgcgtaga tagccgtccc aggcaggacg ctgaaattgc accagatcga gccgataatg
     2041 aaaagcgggc cagagaacgg cattggagaa ttggttgtag tattcgtcaa ggtcctgttc
     2101 gctgaggtta aaagaggccc acgtaatgtt accttttttc acctttttta gcggctgatc
     2161 ctcattccct gtttcaccac tccagccaaa ccacagtccg cctgcggctt tcagtgcccc
     2221 cagtatgcca acggcaaggc caccggcact ggcggcgtgc tcgtctggtg gtgcaatccg
     2281 gttagatact acgactaaac gactcatagt catcactcct gttattttct cttttttgtt
     2341 gtaatgcggt ggttatcatt tcaagccagc tccagacatc cggcacaccc gccagtcgcc
     2401 atgatgcctg agttgcacct gtgccaattt ttactgacat tccgcccagt cggttaacga
     2461 ctgcgaagcc agattcatcg gttaaatcat cgcccagaaa tacgggcgtt cgcccgataa
     2521 agggagcttc ctgcataaaa gctgcaattg cctcaccttt actggtacct ctcggtttga
     2581 tctcgacaac acactttccc tgctgtaacg ccatttgtgg ccagatctga gtaatacgtt
     2641 gcgctaatgt cattaatgcg tcttcatgct gcggagcctg acgataatgc agcgcaaaag
     2701 ccatcccttt cgcctccagc tccgcgccgg gatactgagc gatgactgta tgcagttgca
     2761 cgctaatatc acgcgcaatc gcatccggca gatgaacgat atgtgtttta ccattgatgt
     2821 cacggcgctc cgccccatgc acgcccgcta acgggaagcg ataaggtttt gccagtgcgt
     2881 caagctccac cattgagcgc cctgatatca atgccaatgc accatcactt gcggttgcca
     2941 gtagctgtag tccttgcaga atattgtcag gcacgacgac ctgatcggga tgcggtttga
     3001 tttccgccag cgttccatca agatcaaaaa accaggcata tttcgcggat agttcagggg
     3061 tttcggttaa cggttctgtc acccggctct cctccttctc ttttcttatt gttttgcgca
     3121 gaacgcgctt tacagacatg taagtataga cagtgtgacg ggggtcgcca tttgcaatga
     3181 agaacagcca accgttgtgg cggctggctg tggtgggaaa aaacgttaaa ttgttgtgga
     3241 aaaaagcatc agacaatgcg tttcgctttt tgcttgtaac ggtcgaagat caccgctgcc
     3301 agcaggatta agccgcgaac cacgtactgc gcgaaaggag aaatattaag caggttcatg
     3361 gcgttttcca cggtgcctaa aattaagata cccgccacca catatgagat ttttccgatg
     3421 ccacctttca gagaaacgcc acctaaaacg caggcggaga taacaataag ctcataacca
     3481 atcgacgtca ttggctgccc actagtcata cgtgaagcca gaataattcc ggctatcgca
     3541 gataccaggc cagagagaac aaagataata attttggtgc gaacaaccgg tacacccgcc
     3601 agacgcgcag cctcttcgtt cccgccaatc gccagggtgt tacgaccaaa ggtggtttta
     3661 ttcagcaaca aaccaaagat aatcagacac gcgacggtga gccagattgg cgcaggcaga
     3721 ccgaaccagt tagcgtaacc aagggcaaag aagctttcat cttcgatacc gaccgcttta
     3781 ccgtctgaaa tgatgtacgc cagacctcga acaatctgca tcgttgccag tgttgtgatc
     3841 agagcattta ttttcagttt ggcgataaca aagccattga ccaggccaca gagaatgcca
     3901 agcagcaacc ccgctgccac gccaatccac aggctttcag tcaggttgat aaccaccgcc
     3961 gtggtgacac ccgcacaggc aattacggag gcgacagaaa ggtcaaagtc accggaagca
     4021 aggcagaaca acatgccaca cgccaccatc cccgacatgg aaattgccag gcccaaccct
     4081 ttcatattaa tgaaggtggc aaaatttggg acaaaaatgg cacaggcgat aaagagcacc
     4141 gcaaacacca ccagcatgcc gtactgatcc cagatacgcc cgaagctgaa tgacgactta
     4201 ggtgcgccag aacccgatgt agaaacagaa gacatcatac tctccttact caggcaactg
     4261 cctggctgac tttaggcatc gcaaggctca gtgcctgacg ctcatctgcc tgctcgtgta
     4321 acaattcacc ggcgatttca ccttcccgca tcaccacaat ccggtcggca acgccgagga
     4381 cttcaggtaa gtcgctggag gcaaacagca ccgccacacc ctgcgccgcc agcgcataaa
     4441 ttacgttata tatttcgtgc ttagcaccaa catcaatgct gcgcgtaggt tcatccagca
     4501 aaatgacctt catctcttcc gataaccagc ggcccagaat ggctttttgc tgatttccgc
     4561 ctgagagatt catgatcagt tgctcagcgc ccggcgtttt gatgttgagc gaacgaatgt
     4621 gctgatcggc attgttttct tcccaaccgt tgttgattac acaaccgccg agcacatgtt
     4681 tgcgtctggc actgatgttg atattgtcgc gaacggagtg cacgggaata atgccttccg
     4741 ctttgcgatc ttccgggcag agcatcatgc ctgcggcaat ggcgtggctc ggtttacgaa
     4801 tatcgatcgg ctgttggtcg atataaacct gaccggcggt gatttgcgtc ccgccaaaca
     4861 agcctttcat taattcgcta cgccccgccc ctaccagacc aaacagccca acaatttcac
     4921 cactgcgaac cgccagactt attggcgtac gcacgcctgg tgctttcaca gcatcaagac
     4981 gcaggcgctc ctcgccataa ctacgaggtt gccagccgta gatatcgcca atgtcgcgcc
     5041 cgaccatcgc ctgcaccagc gcgtcgtggt caacctgctg catatcggta aaggttttga
     5101 cataacgtcc atctttaaag acagtgatgg catcgctgag ggcaaatatt tcttccatac
     5161 ggtgagaaac gtataagatc acccgcccct cttttcgcag ttcacgaata acgcggaaaa
     5221 gattgtcgat ttcgcgggca gaaagggagc tggttggctc atcaaaggcg ataattttag
     5281 cgttacgcgc cagcgccttg gcgatttcaa ccatctgcca ctgaccaatg gagagatatt
     5341 tcagcggcgt gtccgggtca atatccatac caagatgttt aagttgtaaa cccgcctcat
     5401 aattcagcaa tgagcgattc acaatgccgc ctttatgcgg cagctggccg agatagatgt
     5461 tttccgcgac ggtcatttcc ggcacgagat gcagttcctg gtaaataatc gccacacccg
     5521 cgttaagtgc tgcggtcgtg tcggaaaagg acatttcctg cccattaatc actacagaac
     5581 ccgtggttgg cgcatagttg ccgctgagga tttttaagag agttgatttt cctgcgccat
     5641 tttcacccat caacgcatga acctgaccgg catagcagtc aaaactaata tccgtcagcg
     5701 ccttaacgcc gggaaacgtt ttaccgatgc cgcgaaatga gagatacggg gtagactgtt
     5761 gcataacgtc tccgtgaatc actggtcgtt cacaccccct ctgcatcatg cagaggggaa
     5821 tttttccggc aaattactta ccgcctaaac cttttttctc cagttcttct ttaaagttgt
     5881 cacgcgtgat cagcaccacg tcggtaactt cggtaaattt tggcggttca acgtcttttg
     5941 ctacccagtt gtaaagcatt tcgctggatt tatagccatg tacgtccggg cttggcagca
     6001 gggaaccgta gaagccggtt gcctgtgctt tagacagttc gctcaacgca tccacaccgt
     6061 taatgccaat gccgatgata tcggcagctt taaagccctg accttccgtc gcgcgtacgc
     6121 cgcccagcac ggtgctgtcg ttcataccga cgatcagcca atgtttaact tccggatgtt
     6181 gaaccagcat tgagttggca gcgtcaaatg cccccgggat gtcgttagat ttggtaggta
     6241 cctgataaat ttgtttttcc gggaatccgg ccgctttcag cgcatccata gaacccgttg
     6301 tacggcgacg tgccgtatcc agttcgttgg cggtaatcgc catcaccgcg ctttctttga
     6361 catcccagcc acgtttctgc atctctttat acagttcctg accctgacgt tcgccaattt
     6421 tagtcgccgc catcatcacc agcggaacgg tatccattgg cttacctttg gcgttaacaa
     6481 actggtcatc cacggcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag
     6541 agccgagttt ggggtccgga gtacaaataa cgaaaccttt tgcgccactg gcagccaggc
     6601 tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc
     6661 ctaaatcctt cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg
     6721 gttgcttcac cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg
     6781 ctgccagacc aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta
     6841 gtgtcgtttt gtgtagggca aaaacgaatg actttcgtta aattaatcgg aaaacaaagc
     6901 attacctttt atctaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt
     6961 ctccatagga gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg
     7021 ggaaaagaca taagggaaag ccaatttgtc agacaaattg tcgaatgcac agcagattaa
     7081 tccataagat tagcctggaa atccttgttg tctttggtac ccatgcggga tgtcttcttt
     7141 ttaaccagtc aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccgc
     7201 aactcaaaga tgtggaaaat gcacgtcatt catttcgcca ttaattatca ctgtgctcat
     7261 taattaacag aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa
     7321 atcatcactt catcatgaat tcaaattcat tgattaatat caacaagata caaaaagcac
     7381 tatcattaaa attcattgca gttacattga tttcatcaat gaaatgtaaa aatatataaa
     7441 cttgatgatt taagcatttt cttatacccg ttcagacgtt attcttattt cagatcatcg
     7501 tcagaattga ctccacgatc acatttcgga ccggcagaaa ggaattattc tgcaaacagt
     7561 aattatggtg ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta
     7621 tggcaaccgc tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat
     7681 ccaatctcta ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca
     7741 ctttccttcg cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta
     7801 tgaagagtgt cggcgctacc cccatcgtta aagccattga tgttcccggt gaaaaactga
     7861 actctctgga agaactgttc caaaaaacga tggaagaata cgagcaacgt tctagtacgt
     7921 tggcacagtt agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc
     7981 gcgatctgga aaaagaacag cagcatgatg gtctgttgct gcaaaccatt cttgatgaag
     8041 tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg
     8101 tcgtgtcaca ccagctgcat tgatcatcat cggcgctaat gcattgcgcc gatgaaggtt
     8161 ttgagaaacc gctgcctcat ctgtttgaag cagcggtttt tttaatggga ttcaccctgt
     8221 ggggtaaact tgagttcaat aagcgcgatg gctttttgga ttgcccgcat ggtgaccggg
     8281 tctgcggcgg cgggatggtt agtaaagtcg atattcttca gctgactgga cattttttca
     8341 cgaacttcaa cgggcgcgat tacatcgaga acatccagaa tttgtttgat aaccaactgg
     8401 caagcaacca catcagaaac cagttcctga tcggcattca gcggctggga catcgtaaac
     8461 tcctgatagc attttgaaag ccgttatagt agcggcttca catcttcagc gatagtcaca
     8521 tccaccgtca tcaggacaca aaaaaacctg ccggagcagg ttttttgtta tcggaacata
     8581 ttgcctggcg gtacgtcttt gaacgtcttg caatagttat tgaacatact tttcaggatt
     8641 ttgcgcagtt tcatcgcggc actccgacca tttgttatac aggtgttatt gtctttgcgc
     8701 ttataatatg acaaccatca caaaaatcaa tctttatgtg atacaaatca cataaatacc
     8761 cctttaatgt tataaaaatg ataatcaaaa aacagccccc ctatttctga cacctacaga
     8821 tggcaagaaa tagcgcctgc caggcgtctt ttccggccat tgtcgcagcg ctgtaacgcg
     8881 taaaatagtg ctttctctta ctcttctggc tggaccatga gacttctgat tctgactctt
     8941 tcattaataa cgctggcggg gtgtacggtt actcgccaag cccatgtgag cgaagttgac
     9001 gcggcaacag gtattgtacg gttggtttat gaccaggctt ttttgcagca tgcccatacc
     9061 gatcgttatg tcagtcgcgg gatcgccgat cgcgcatgcc agcaggaagg ctatacccac
     9121 gcggtcccct ttggtcagcc agtaggcaac tgtagccttt ttgcgggttc cttatgtttg
     9181 aatacggaat tcactttgtc atatcagtgc catcattccg ccttccctgt ctttctctaa
     9241 atattttaat cagcgagggg atcttcgctg attaaagaaa tagcggtaat gcgttttatt
     9301 cccattcgta tttttaataa ttgaagttta tattttacct tttgcaaata ataaaataac
     9361 aaattatagt gacgccacag caacaaatat aacctttgtg gagcactatc atgctgaaac
     9421 cagaaatgat tgaaaaactt aatgagcaga tgaacctgga actgtactct tcactgcttt
     9481 atcagcaaat gagcgcctgg tgcagctatc ataccttcga aggtgctgcc gcgttcctgc
     9541 gccgtcacgc ccaggaagag atgacgcata tgcagcgtct gtttgattac ctgactgata
     9601 ccggcaattt accgcgtatt aataccgttg aatctccgtt tgctgaatat tcctcacttg
     9661 atgaattatt ccaggaaacc tataaacacg aacaattaat cacccagaaa attaacgaac
     9721 tggctcatgc tgcaatgacc aatcaggact acccaacatt taatttcctg cagtggtatg
     9781 tttctgagca gcatgaagaa gagaaactgt tcaaatcgat tattgataaa ttaagcctgg
     9841 caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga actctctacc ctcgacacac
     9901 aaaactaatg ctctccggcg gcaggtttac tgccgccgtt tcaaatcagt gacggcaaat
     9961 cttactctga tcggtcgaaa aaccgtcttt cgaaggtata aatttgcctt ttgctgccag
    10021 aaacgctacc agctctcccg ctgtcatccc ttctgccgag caggtgtgaa aacgtgcctg
    10081 ttcaccaaaa cgcgctttaa tcgcagcttc cagactggca tgcgtatatt gctcgcctga
    10141 ttcaatcatc atatttaaca cttcatgacc gtgaatagag tccatcgttc ctcctcaaaa
    10201 aaagcctagc gtagcgattg ccgcttatga agactttgcg ccagcgcagg actgaatgct
    10261 ttttattgta catttatatt tacactatat gtaacgtcgg tttgacgaag cagccgttat
    10321 gccttaacct gcgccgcaga tatcactcat aaagatcgtc aggacagaag aaagcgtgaa
    10381 aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc acaattggcg caggcatgct
    10441 ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt acgttaatct tgttgattgg
    10501 gctttgggcg ttgatgtgct acacggcgct attactgctg gaggtgtacc agcatgttcc
    10561 ggcagatacc ggtctgggca cgctggcaaa acgctatctg ggacgctacg gtcaatggct
    10621 gacgggcttc agtatgatgt tcttaatgta tgctctgact gcggcataca tcagcggtgt
    10681 cggtgaattg ttggcctcca gcatcagcga ctggacaggt atttcaatgt cggcaaccgc
    10741 tggcgtgctg ttgttcactt ttgttgccgg tggcgtggtt tgtgttggaa catcgctggt
    10801 cgatttattt aaccgttttc tgttcagcgc caaaattatt tttctggtgg tgatgctggt
    10861 attgctgcta ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc aacaggggct
    10921 ggctctgtca gcaatcccgg tgatttttac ctcatttggt tttcacggta gcgtgccgag
    10981 tattgtcagc tatatggatg gcaacgttcg taagctacgc tgggtgttta taaccggtag
    11041 tgcgatcccc ctggtggcat atattttctg gcaggtggca acgcttggca gcattgattc
    11101 aacaaccttt atgggattgc tggctaatca tgctggatta aacgggctgt tacaggcgtt
    11161 acgcgaaatg gtggcctctc cgcatgttga gctggcagtg catttatttg ctgatttagc
    11221 cctcgccacg tcatttctcg gcgttgcgtt aggcttattt gattatctgg ctgatttatt
    11281 tcagcgttca aataccgttg gtggacggtt gcaaactggt gcaattacct ttctgccgcc
    11341 gttggcgttt gcactgtttt atccacgagg atttgtgatg gcgctgggtt acgccggtgt
    11401 ggcgctggcg gtactggcat tgattatccc ttcgctgttg acctggcaaa gcagaaagca
    11461 caatcctcag gcgggttacc gggtcaaagg tggtcgtccg gcgctggtgg tggtgtttct
    11521 ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg gcagggttgt taccagaagt
    11581 ggggtgatca gatagcctca aattccttat tgggtgccag aattaacgct gacacccaat
    11641 ttggcctctt aatgcaggca gcactgctta aatttcttac cactaccgca cgggcaagga
    11701 tcgttacgcc ccggtttctc ttctgctttg atcggttgct gaacagcttt ttcctgcgga
    11761 tgcgccatcc agtacgcatg tagatcaagc gccgccagtc gaatggcatc tacgctctct
    11821 tcaaacgctt ctggcgacat cttttctacc cgctcgaagt tttcctcagt accgtgcagc
    11881 gcaatcgcct ccagcgctgg ttttaacgaa tcgggcaacg ttgaccagtc agaaagtgcc
    11941 acgccccgca tatagccaaa gcaccactcc tcaacaatcg tcagctcgct gccatcaact
    12001 tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt cgttcagacg ctctgcggta
    12061 tcggccatat gttgaaaagc caggttcata aagcgcgtca tctctttctc tgacgcccag
    12121 cgcggcacat agtcagcccc accccacacg gcaaccagcc actgttccgg ttcaatctct
    12181 tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc aaggatggcg
    12241 tggttagtgt tgtatttggt cagaatatcg tccagccatt ccaactcact ttcgtttaac
    12301 ggtcccgttt tcatacgctt ttccttgtgg atctcaactc gccagcacct atcttacatg
    12361 ccggtccgta tcagagatac tttttgagtg gctttgccgg tgattaaaaa ttaaggaggg
    12421 tgtaacgaca agttgcaggc acaaaaaaac cacccgaagg tggtttcacg acactgctta
    12481 ttgctttgat tttattctta tctttcccat ggtacccgga gcgggacttg aacccgcaca
    12541 gcgcgaacgc cgagggattt taaatccctt gtgtctaccg attccaccat ccgggctcgg
    12601 gaagaaagtg gaggcgcgtt ccggagtcga accggactag acggatttgc aatccgctac
    12661 ataaccgctt tgttaacgcg ccaaattctt caggcctttc agccagacat ccgcttgacg
    12721 ccgatgtctt ttaaactgga gcgggaaacg agactcgaac tcgcgacccc gaccttggca
    12781 aggtcgtgct ctaccaactg agctattccc gcattcatca agcaatcagt taatcacttg
    12841 attttattat cgtctggcaa tcagtgccgc cgttcgatgc gttgcattct acttacctgg
    12901 cgcgatgagt caacgatatt tttcaccact tttgatcgtt tgctgaaaat tacgccgaaa
    12961 cgatcactga tcaagcaaat ctgcacgcgc agcgctcaaa tattgcaaca ttgaccacag
    13021 agtcagtacc gcagccacaa agaaaagtgc aataccggcg tactcaaccc aaatgttcgg
    13081 acgccacagc agccatgcca acgccaccat ctgggcagtg gttttcactt tcccaatcca
    13141 ggagacagcc acgctactgc gtttacccaa ctccgccatc cattcgcgta gcgcagaaat
    13201 aataatttca cgggcgatca tcgttgctgc cggtaaggtc acccaccagc tgtggtaatg
    13261 ctcggttacc agcaccatgg cgatagccac gagaacttta tctgctacag ggtcaaggaa
    13321 agcaccaaac cgggtactct ggttccagcg gcgtgccaga aaaccatcga accagtcagt
    13381 caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc gaccaggtga caggcagata
    13441 aaagaccaat acaaagaatg ggataaggat gacacggaac agtgtaagca acgtagggat
    13501 attaaattgc ataatgacgg gtaactatct gttgtcagta agattacccc tatgttgcta
    13561 cagagacatc aatgtttcaa cgaccagaag atcttttctg ccagaccttg cgaaataccc
    13621 ggcacttttg caatttcctc gacgctggcg ttacgtaaac cttgcaaacc gcccatatat
    13681 ttcaacaaca tttgccgacg ttttggcccg acgccttcaa tggtttccag ggaactggta
    13741 tttttgacct tcgcccgttt tttacggtgc ccgccaatcg cgtgatcatg tgattcatcg
    13801 cgaatatgct ggataacatg cagcgcggga gaatctggcg gcaaactgaa tccctcacct
    13861 tccggctcaa agaacagcgt ttccagccca gccttacgat ctgctccttt ggcaacgcca
    13921 agtagcagcg gatgattttt atcccatgag acatccagtt cggcgaagac atttttcgcc
    13981 tgcgcaagct ggcctttgcc gccgtcgata aggatcacat ccgggatctt actgtcgtca
    14041 atggctttac cataacgccg acgcagcacc tgattcatcg ccgcataatc atcgcccggc
    14101 gtgatgcctg taatgttata gcgccgatac tccgcacgca gcgggccgtt agcatcaaac
    14161 accacacagg aagcgacggt ttgttcgccc atggtatggc tgatgtcaaa gcactccatc
    14221 cgcttcactt ccggcaattt caacacgctg gcaagcgcgg tcagtcgctg gtgaacggta
    14281 gattgctgcg aaagtttgct ggttaaggcc gtcgccgcat tggtgcgcgc gagtttcaga
    14341 taacgcgccc tatcgccgcg aggtttggtt tgaacattaa tcttgcgtcc cgccagttct
    14401 gaaagggaat cggcgagcag cgttttatcg ctaagattaa aatcgagcag gatctcaccc
    14461 ggtaaggtgc gcatctggct gccttgtaaa tagaactggc ctacgaaggt ttctaccacc
    14521 tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc tgcggctgcc gagcactttg
    14581 ccctgacgaa tgaacaatac gtggacacaa gccatgcccg catcgaacgc cacaccaata
    14641 acgtcgaggt cgtcgccggt attggaaacg aattgttttt cggtgacgcg tcgcaccgcc
    14701 tgaatttggt cgcgaatacg tgcagcttct tcaaactcca gattctggct ggcagtttcc
    14761 atacgactaa tgagttgcgt aagcacctga tcatctttgc cagacaaaaa caggcgcaca
    14821 tactcaacct gctgagcgta ttcttcttca ctcaccagtc cttcaacgca cggtcccaga
    14881 cagcgcccta tctgatattg cagacacgga cgcgagcgat tgcgataaac actattttcg
    14941 cactggcgaa tggggaaaat cttttgcagt agcgccagtg tttcacgtac ggcatagcca
    15001 ttcgggaacg ggccgaaata ttcacctttg gcatgcttcg caccacgatg catcgccaga
    15061 cgcgggtggg tatcgccact caggaagata aaaggataag atttatcatc gcgtagcaaa
    15121 acgttgtaac gcggctgata gagtttgatg tagttgtgtt ccagcaacag cgcttcggtt
    15181 tctgtatgcg taaccgttac atcaatttgc tggatctggg cgaccagcgc ttcggttttg
    15241 cgcgaagcga ggttgctgcg gaaatagctg gaaagccgtt ttttcaggtc tttcgctttg
    15301 ccgacataga taaccgtacc accagcatcg tacatgcgat aaacgcctgg ctggcaggtt
    15361 acggttttta aaaacgcttt tgcgtcaaac tgatcactca ctgacttgat aatgtctccg
    15421 cattacacag accatggcga attgccaggt gagtcagctc aacatcgcca tgaatgttta
    15481 gtttactgaa catacgatag cggtagctgt tcaccgtttt cggactgaga ttgagctgtt
    15541 ctgagatctc attgaccttc tggcccttgg tgatcatcag cataatctgc aattcacgtt
    15601 cagacaaact ggcaaatggg ctttctgttt tttctggttc gatctggctt aacgccattt
    15661 gttgagcgat atcagaagca atgtaacgct gccctgaata gacagaacga atcgcactca
    15721 cgacttcctg cggagccgcg cctttgctga ggtagcccgc agcaccggcc tgcatgactt
    15781 tcgctggtaa agggttttct gtatggacgg taagcatgat gattttgaca tcagctgtgg
    15841 aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc cggcatactc atgtccatta
    15901 gcaccacgtc aacggcattt gtccggcacc acttaacggc gtcttcaccg cacgatgcct
    15961 caccgacgac ttttataccc tttatatctt ccagaatgcg tcgtatccct gcgcgcacca
    16021 gttcgtggtc atcaacaagt agaacgttga tcaaaggaat atctccagaa atagggataa
    16081 cgctactgat agttagtcat tcgtatatta gcggttttta ttgcaacttt gaaacgttaa
    16141 aaatggtggg tattcgattt ttctctcgtt tttgtaaatt cgattgtcca cagttatggt
    16201 cacgcccgtc accaacggct tccgggatcg tttcacagta agcccggcga tgaaatgtga
    16261 caaaaattga ctttattcag caaaaatgaa aatcagcccg ctgaatatgt aacattaatt
    16321 aacccgaaca atacgggcaa aaaacattca tgataaaaat atttattggt cattatatta
    16381 acgcatttaa tagcattgtt gacgtcacgc tcaaaaaaca accactgcta tttttagcaa
    16441 agcttatggt atactccgcc gccttaacat ttttcaccgc aaattttcat tgcaacatga
    16501 cgaggaaaat aaatgagtac gcctgatttt tctactgccg agaataatca agaactggca
    16561 aatgaagtct cctgcctgaa agcgatgctg acgctgatgc tacaggcgat gggacaagct
    16621 gacgcgggcc gcgtgatgtt aaagatggaa aaacagcttg cgctgatcga agacgaaacc
    16681 caggctgcag tattttccaa aacggttaag caaattaaac aagcctaccg tcagtaatat
    16741 aaaaccggct gagagcgtgc ctttcagccg atttttgcat ctggcacgca gaaagaaaag
    16801 cgatcaaatt aagccagtag cggccgcgta acaggccacc tgggtcttat ttggtgcatt
    16861 gatttttttc tgcatgtttt tctgatggaa attgaccgta ttctcagaga ttgacaaaat
    16921 catcgctatc tctgctgatg tcttcccttc cgccgtccat ttcagaattt ctttttcgcg
    16981 cttgctgaaa ttcatctctg gcgtcatcac tatttcatca tttaaacgca tcagagccat
    17041 cagactttcg cgcaccagta actgcatttt taattgcagt tcatcactaa gaatgggtat
    17101 ttcgcgcgtg ctgcaacggg aaaaggacaa aaagcccagc gcccgatttg gcagcattaa
    17161 atactgagtg acaccgcggc gtaaaccatg tgcgtgcgcg gcttcccata atggctgtgc
    17221 ttcgctgaat aagtcatcat tccacattaa atggccctga ctaaagtttt cagggttcag
    17281 cacaggatca attgcgagaa aattttttgc ctgataataa ctaacccacg actcagggta
    17341 attggtgtag aaagccactt taggtcgcgt gaatggtacc gggtggcgaa cacataacga
    17401 atagtaatcg tactccagct gctgagcctg aagctctatt tcatggtaga cctcttctgc
    17461 ggcctccatc ctctgaaaac gcaacagcat cgtgcgacgc cagctgaaaa aatccgtatc
    17521 ctgcatagta aaccgcaacg cccctgagag tgagtatcat ttataatgat aattcaaact
    17581 atcacataaa taagatttat atataattta tattattcag gcaatgaatt acttttgcaa
    17641 gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca gttttatcac
    17701 gcttgaaatg gcgtcccgcc ccgacaggtg aatcgtcggg gctgattttt tcttattatt
    17761 gcagtagaaa cttctcgagg aactggcggg tgcgaggctg ctggggatca gcaaataacg
    17821 cttttgctgc cccctgctcg actatccgcc cctggtccat aaaaatcgcc cggtccgcaa
    17881 catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca
    17941 gctggcggat tgtgttcagg acttcgccca ccagctctgg atccagcgct gacgttggct
    18001 cgtcaaacaa aatcacctca ggacgcattg ccagcgcacg cgcaatcgct acacgctgct
    18061 gttgaccgcc agacaaacga cgtggatagc tggtttcttt accggccagc ccaactttcg
    18121 ccagcagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg acgatcaccg
    18181 gcccttcaat aatgttttcc agcaccgtac gatgcggaaa caaattaaag ttctggaaga
    18241 caaacccgac gtgctgacgc aactggcgga tcagcgattt ttgctgactt aatgaacgtg
    18301 cagtatcaat agtgatatcg ccaacggtga tcgttcccgc ttccggttgt tccagcagat
    18361 ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataatt gccaccactt
    18421 cgccaggctt tacctcgagg tcgataccgt gcagcaccgt ctgaccgtga aattttttca
    18481 ccaggttctt aacttcaatg gcactcattt tggttctctc tcctggcgat taagttgatt
    18541 ctcaaaatgg ttctgcaacg tcgataatac tgtcgccatg atccagtaga tgagcgaagc
    18601 cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg cctgacggaa
    18661 cagctccggc acctggattg tcgcggccag cgacgtatcc tttaccaggc tgataaagct
    18721 gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatagt
    18781 ctgccatggc gtcataccaa tactggcagc tgcttcccac tgacctttat caatagaaga
    18841 aatggcggcg cgcagtgttt cggcggcata ggcggcagtg ttcagcgaca gaccaatcat
    18901 tgctgacgga atgggatcta attcgatacc aaactgcggc aggccgtaat agatcataaa
    18961 cagttgtgcg atgagcggtg taccacgaaa aatagagata taaaaacgcg ccagccagcg
    19021 cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagca gtaagccaaa
    19081 aaacatgccg ccgatactga gttgcagcgt atacccagcc cctttcagca ggaacggcaa
    19141 ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta aacgtgggga
    19201 tgataggcga acagcgcagg cgcaccgccg gtatgaataa acagaatcgg cccttcatct
    19261 ttgaagcgtt tctgactgat acagccaatc agccccgcca tcgcttttcc ggtatacaca
    19321 ggatcaagca gaatgccttc cagccgcgcc agcaatttca ctgcttccat gccttcgtcg
    19381 ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtataatttc cgctgatgcg
    19441 gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg caactgatcg
    19501 gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc
    19561 ccaacagcca gtccggcgtg agttccggca ctgcccgatg cgactaccac cgacgaaata
    19621 ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac tctccacata acctagcgcg
    19681 ccaagagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttcgact
    19741 cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcttcgca catttcaatc
    19801 tgggtattga acagatccag caacaagcga ttgccgttgg ttaaatagtt ttctgcggtt
    19861 gtgccaatag gattttccag cagcgccaca cagtgcagac cgagtttcgc cgcaactgcg
    19921 gcagtctgcc gcacatggtt agactggatc accccggcag taatcagcgt atcggcacct
    19981 tcgcgcagag catctgccgc gagaaattct agcttacgta atttattgcc gcccattgcc
    20041 atgggggtga cgtcatcccg tttgatgaaa atttcccgtc ctagataatc agaaaagcgc
    20101 ggcagatatt cgagcggcgt tggcgcgccg ataaactcca accgtggaaa acgggttaaa
    20161 ttatgcagtg gcataacagc ctccgatgta tgttgttgtg attttcttat tatgcacgct
    20221 gaaaacgcgt aa
//
LOCUS       DAJTYZ010000076        20232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000076 DAJTYZ010000000
VERSION     DAJTYZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.076"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            691..1461
                     /locus_tag="OO596_002569"
     CDS             691..1461
                     /locus_tag="OO596_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6718398.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1452..1925
                     /locus_tag="OO596_002570"
     CDS             1452..1925
                     /locus_tag="OO596_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6718399.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2033..2572
                     /gene="dnaT"
                     /locus_tag="OO596_002571"
     CDS             2033..2572
                     /gene="dnaT"
                     /locus_tag="OO596_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6718400.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2575..3312
                     /gene="dnaC"
                     /locus_tag="OO596_002572"
     CDS             2575..3312
                     /gene="dnaC"
                     /locus_tag="OO596_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6718401.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3361..3855
                     /locus_tag="OO596_002573"
     CDS             3361..3855
                     /locus_tag="OO596_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6718402.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4109..6400
                     /gene="opgB"
                     /locus_tag="OO596_002574"
     CDS             4109..6400
                     /gene="opgB"
                     /locus_tag="OO596_002574"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6718403.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6539..7561)
                     /gene="lgoD"
                     /locus_tag="OO596_002575"
     CDS             complement(6539..7561)
                     /gene="lgoD"
                     /locus_tag="OO596_002575"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6718404.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7700..8614
                     /locus_tag="OO596_002576"
     CDS             7700..8614
                     /locus_tag="OO596_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6718405.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8829..10180
                     /locus_tag="OO596_002577"
                     /pseudo
     CDS             8829..10180
                     /locus_tag="OO596_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10229..11884)
                     /gene="tsr"
                     /locus_tag="OO596_002578"
                     /pseudo
     CDS             complement(10229..11884)
                     /gene="tsr"
                     /locus_tag="OO596_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12003..12449)
                     /gene="hpaR"
                     /locus_tag="OO596_002579"
     CDS             complement(12003..12449)
                     /gene="hpaR"
                     /locus_tag="OO596_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6718406.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12721..14010
                     /locus_tag="OO596_002580"
     CDS             12721..14010
                     /locus_tag="OO596_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6718407.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14007..15473
                     /gene="hpaE"
                     /locus_tag="OO596_002581"
     CDS             14007..15473
                     /gene="hpaE"
                     /locus_tag="OO596_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6718408.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15475..16326
                     /gene="hpaD"
                     /locus_tag="OO596_002582"
                     /pseudo
     CDS             15475..16326
                     /gene="hpaD"
                     /locus_tag="OO596_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
     gene            16336..16716
                     /locus_tag="OO596_002583"
     CDS             16336..16716
                     /locus_tag="OO596_002583"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6718409.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16827..17630
                     /gene="hpaH"
                     /locus_tag="OO596_002584"
     CDS             16827..17630
                     /gene="hpaH"
                     /locus_tag="OO596_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6718410.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17641..18429
                     /gene="hpaI"
                     /locus_tag="OO596_002585"
     CDS             17641..18429
                     /gene="hpaI"
                     /locus_tag="OO596_002585"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6718411.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18604..19980
                     /gene="hpaX"
                     /locus_tag="OO596_002586"
     CDS             18604..19980
                     /gene="hpaX"
                     /locus_tag="OO596_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6718412.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19990..>20232
                     /locus_tag="OO596_002587"
     CDS             19990..>20232
                     /locus_tag="OO596_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6718413.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4870 a         5430 c         5169 g         4763 t
ORIGIN      
        1 ctcttttaac cctgcttgca taacaggtat tttactgata acaattccat ttttgcagca
       61 tcctggcaac atatttcctc cacatccact gtctaaaaat catgatggtt tattcaggat
      121 attcattcct taaatgtttc cagcaaatta atagattaag cgaataaatt atccttagca
      181 ttcactttca tatcagcata ctcctgctgc acaacaactc cactgcatgt gtcagaaggt
      241 tagcatcaaa tgataacctg gtgaaatagc ctggagacat ttaaaatatc tacattaagt
      301 tgttaggaat acttaatgaa aaacagaaat cacattcatt taatttataa ttaaatgata
      361 tcccttcccg cagaatgaaa aatcacacaa ccaatattta ttcaatgaaa tctgatggtt
      421 ttaacttgtt aacattaaaa aacgtgcccg gttatctatt atcctgattc gtaaaggcca
      481 aacaataaat tcattatagt gataatattc atgtgatagt ttgtttgcgg cgagagataa
      541 ttcgcttttt atcaccgagc ttgtcccgat aaccaggatt aatcagcgca attttgatct
      601 taacctaccg caaaacaacc gtgcttcatg atacaaatgg attctcatta tctgaacaat
      661 acgcagcacg tctatgacaa agggcgagtt atgcaaactg agcaacagcg agccgtaaca
      721 cggctttgta tccagtgtgg attatttctt ttgcaacatg gtgcggaaag cgcgttggtt
      781 gatgagcttt cctcacgact gggtcgggcg ttggggatgg acagcgtcga aagttctatc
      841 tcttcgaacg ccatagtgct gacaaccatt aaagatgggc aatgcctgac atcgacacgt
      901 aaaaatcacg atcgcggcat taatatgcat gtggtgactg aagtccagca cattgtgatt
      961 ctggcggagc accatctgct ggattacaaa ggcgtagaga aacgatttag ccaaattcag
     1021 ccattacgtt acccaagatg gctggtagcc ttaatggttg gcctttcttg cgcctgtttc
     1081 tgcaaactca ataaaggtgg ctgggatggt gccgtcatca ccttctttgc cagtacggcc
     1141 gcgatgtata tccgccagct gctggcacaa cgtcatcttc atccgcagat caacttttgc
     1201 ctcaccgctt tcgccgccac caccatttcc ggattgcttt tgcaacttcc cactttcagc
     1261 aatacaccca ctatcgcgat ggccgccagc gttctgctgc tggtgccggg atttccgttg
     1321 attaatgccg tcgccgatat gtttaaaggc cacatcaata ccggactggc acgctgggcg
     1381 atcgccagtc tgctgacact ggctacctgc gtcggcgtgg tgatggcact gacgatttgg
     1441 gggctacgcg gatgggtgtg atcgagtttc tgtttgcatt ggcgcaggat atgatcctcg
     1501 ccgccattcc agcggtcggc tttgcgatgg tgttcaacgt tcccgttcgg gcattacgct
     1561 ggtgtgcgct gcttggcgcg attggtcatg gttcacgaat gatcttaatg accagcgggc
     1621 tgaatattga gtggtcaacc tttatggcct ccatgctggt cggcaccatt ggtattcaat
     1681 ggtcgcgctg gtatctggcg catccgaaag tgtttaccgt ggcggccgtt atccctatgt
     1741 tcccgggcat atcggcttat accgcaatga tttcggcggt aaaaatcagc cagttaggtt
     1801 acagcgaacc gttgatgatt accctgttaa ccaactttct tacagcttca tcgattgttg
     1861 gtgcgttatc catcggtctt tccattcctg gattatggtt gtaccgcaag cgccctcgcg
     1921 tataaaattg cctcgcttaa agcacacgga tgagagacag cctcctctcc tccgtgtgtt
     1981 actataaaag ttatctccct tctcgttcat cgttccatat ttgagaaaca gtatgtcttc
     2041 cagagttttg accccggacg tcgttggtat tgacgccctg gtacacgatc accaaaccgt
     2101 tctggcaaaa gctgaaggcg gtgtggttgc cgtatttgct aacaatgccc cggcgtttta
     2161 tgccgtcacg cctgcacgcc tggctgaact gctggcgctg gaagaaaagc tggcgcgtcc
     2221 gggaagcgat gtcgctctgg acgatcaact ctatcaggaa ccgcaagccg ctcccgttgc
     2281 tgtacccatg gggaaattcg ccatgtatcc ggactggcaa cccgatgccg attttatccg
     2341 cctggcggcg ctatggggtg tggcactaag agagccggtg accaccgaag aactggcctc
     2401 attcattgcc tactggcagg cggaaggtaa agtctttcac catgtgcagt ggcaacaaaa
     2461 actggcgcgc agcctgcaaa tcggtcgtgc cagcaacggc ggactgccga aacgagatgt
     2521 gaatacggtc agcgaacctg acagtcaaat tccaccagga ttcagagggt aacgatgaaa
     2581 aacgttggcg acctgatgca acgcctgcaa aaaatgatgc ctgcccatat caaacctgca
     2641 ttcaaaacgg gtgaagaact actggcctgg cagaaagaac aaggagcgat ccgctccgcc
     2701 gctctcgaac gtgaaaatcg ggcgatgaaa atgcagcgca cctttaaccg ctccggtatt
     2761 cggccactgc atcagaactg ctcctttgag aactatcgcg ttgagtgtga agggcagatg
     2821 aatgcgttaa gcaaagcgcg ccagtacgtc gaagagtttg acggcaacat cgccagcttc
     2881 atcttttctg gtaagccagg aaccggcaaa aaccatctgg cggcggcaat ctgcaacgag
     2941 ctgctgctac gcggtaaatc cgtattgatc atcaccgtgg ccgatattat gtcggcgatg
     3001 aaagatacct tcaggaatag cggtaccagt gaagaacaac tgcttaacga tctgagcaac
     3061 gttgatctgc tggtgatcga tgagatcggc gtgcagaccg aatcgaaata cgaaaaagtg
     3121 atcatcaacc agatcgtcga tcgccgctct tcttccaaac gcccaaccgg gatgctgacc
     3181 aacagtaata tggaagagat gaccaagttg ctgggcgaac gcgtgatgga ccgtatgcgc
     3241 ctgggtaaca gtttgtgggt gatcttcaac tgggatagct accgtagccg ggtaacaggt
     3301 aaagagtatt aatccccgtt tctggcggta tactgcgaca caattccacc ttgagtaatg
     3361 atgatgaaaa ctgttaaaca tcttctgtgc tgtgccattg ccgccagcgc attaatttcc
     3421 accggggtgc atgctgcgtc ctggaaagat gcgctctcca gcgccgccag cgaacttggc
     3481 aaccaaaaca gcacgacaca ggaaggcggt tggtcgctcg cgtcattaac taacttgctt
     3541 agcagcggga accaggcctt aagcgcagat aacatgaaca acgccgcagg cattctgcaa
     3601 tactgcgcga agcaaaagct ggcgtcggta accgatgccg aaaacatcaa gaaccaggtg
     3661 ctggaaaagc tgggcctgaa cagtgaagag caaaaagaag acaccaacta tctggatggc
     3721 attcagggtt tgctgaaaac caaagatggt cagcaactca atctggataa catcggaacg
     3781 actccgctgg cagaaaaggt gaaaaccaaa gcctgcgatc tggtgttaaa gcaggggctg
     3841 aacttcattt cctgaaccgc gaaacgccgc gttttagctg ttacccggct gacgcggctc
     3901 tctcctgccc ttttccctgc tcctggacgg ttttccccct gttacacggg aagcgaccgc
     3961 gatcaaaaaa gtctgtttat aaactaattc ctaacaacat cgcatttttt tgacactaga
     4021 attgcagcaa tatggcgacg ccattacatt gtgcttctca aaaagcttaa acgttggcta
     4081 aacggcccgg tttagtgagg atcgcccgtt gtcagaacta ctctctttcg ccctttttct
     4141 cgcctctgtg ctgatttacg catggaaagc gggacgtaac acctggtggt ttgcagccac
     4201 attaacggtg ctggggctat ttgtcgtttt aaatatcacc ctgtttgcca gcgactattt
     4261 tactggcgat ggtattaacg acgcggttct ctatacctta accaacagcc tgaccggtgc
     4321 tggcgtcagc aaatacatcc tgccgggtat cggcattgtg ctggggctga cagcggtgtt
     4381 cggtgcgctg ggctggatcc tgcgccgtcg tcgccatcat ccgcaccatt ttggttacag
     4441 cctgctggcg ctcttactgg cgctgggttc ggtggacgcc agcccggcat ttcgtcagat
     4501 aacggaactg gtgaaatccc agtcacgcga tggcgacccg gactttgcgg cttattataa
     4561 agagccgtcg aaaactatcc ctgacccgaa actcaacctg gtttatatct acggcgaaag
     4621 tctcgagcgg acctattttg ataacgaagc tttcccggat ctcacgcctg aactgggcgc
     4681 gttgaaaaat gaaggcctgg atttcagcca cacgcagcag ctgccaggaa cggattacac
     4741 gattgcgggc atggtggctt ctcagtgcgg cataccgctg tttgccccct ttgaaggcaa
     4801 cgcctccgcc tctgtctcca gcttcttccc gcagaacatc tgtctgggcg atatcctgaa
     4861 aaactcgggt tatcagaact atttcgtgca gggcgcgaat ctgcgttttg ccggtaaaga
     4921 tgtgttcctg aagtcgcatg gcttcgacca cttatacggc tcagaagagc tgaaaagcgt
     4981 ggtggccgac ccgcactatc gcaacgactg gggattctac gacgataccg ttctcgatga
     5041 agcgtggaaa aagtttgaag agctttcccg ctcaggtcag cgattctcac tgtttaccct
     5101 gacagtcgat acccatcacc cggatggttt tatctcgcgt acctgtaacc gcaaaaaata
     5161 tgattttgac ggtaagccga accagtcatt cagcgcggta agttgcagcc aggagaatat
     5221 cgcggcgttt atcaacaaaa tcaaagcgtc accgtggttt aaagataccg tcatcgtcgt
     5281 ctcttctgac catttagcga tgaacaacac ggcgtggaaa tacctcaata agcaggatcg
     5341 caataacctg ttttttgtca ttcgtggcga caagccgcag caagagacgc tggcagtgaa
     5401 gcgtaacacg atggataacg gcgcgacggt gctggacatt ctcggtggcg ataactatct
     5461 cggacttggt cgtagcagtt tatccgggca gtcgatgtcg gaaatcttcc tcaatatcaa
     5521 agagaaaaca ttggcgtgga agccggatat catccgcctg tggaaattcc ctaaagagat
     5581 gaaagagttc accatcgacc agcagaaaaa catgattgcc ttctcgggta gccatttccg
     5641 tttgccgctg ctgttgcggg tttcagacaa acgcgtggaa ccgctgccgg aaagcgaata
     5701 ctcagcaccg ctgcgtttcc agctggccga tttcgctcca cgcgacaatt tcgtctgggt
     5761 tgaccgttgc tataagatgg cacaactctg ggctccggaa ctggcactct ccaccgactg
     5821 gtgtgtctcg caagggcagc ttggcggtca gcaaattgtt cagcatgttg acaaaacaac
     5881 atggcagggc aaaacggcat ttaaagatac ggtcatcgac atggcgcgtt ataaaggcaa
     5941 tgtcgatacg ctgaaaattg ttgataacga tattcgctac aaagccgaca gtttcatctt
     6001 caacgtcgcc ggtgcgccag aagaggtgaa acagtttagt gggatttccc gtccagagtc
     6061 gtggggccgc tggtccaacg cgcagctggg cgatgaagta aaaatcgagt acaagcatcc
     6121 gctgccaaag aaatttgacc tggtgattac cgccaaagca tacggcaata acgccagccg
     6181 tcctattccg gtacgcgtag gcaatgaaga acaaaccctt gtgctgggca atgaagtgac
     6241 caccaccaca ctgcatttcg ataacccaac cgatgccgac acactggtaa ttgtgccgcc
     6301 ggaacctgtc tcaaccaacg aagggaatat cctcggacac tcgccgcgta agctcgggat
     6361 cggcatggtg gaaattaaag tggtggaacg tgaaggataa ttcctgactg atgtgtaggt
     6421 cgcataaggt gcgacctaca caccttctgc ggcctacaaa acatcattga gaaacagaaa
     6481 aaaacagcgc ccaacacttc cccggacgct gcatggcaac tttaataatc tcgccttatc
     6541 agaaagtaat tacaccctta atcaactcac gattgttaat cacatcgcgc tcgtaggttt
     6601 ctgccagcgt ggcgaacgga tagcgatggg ttaacatcat gtcagcagtg atttttcctt
     6661 ccgccatcag tcgaccgact ttagcaaaat cttccggcgt ggcgttgcgg ctacccatca
     6721 tcgtcgtttc ttttttatgg aattccggat cggagaactg caactcacct ttaaacaggc
     6781 cgacaaatac caccgtgccg ccgtgacgaa tcaaattcac ggtgttattc atcgcatgtt
     6841 gattacctgt cgcgtcgatc actttctgcg ccagcgaacc accaaactgc gcccgtagct
     6901 gcgcgtcaaa atcttcggct gacgggtcca gtaaaggtaa ttccagacgc gttgccacat
     6961 gttcacggcg cgccggactg gtatccgcca ccaccacctg tgcgccatcg gctttagcga
     7021 ttgccgccgc gcccagaccg attggccccg ccccgaccac cagcacctgc tcgccgggag
     7081 caatggctgc gcgacgcacc gcatgagcgc taatagcgaa aggttcaatc aatgctgccg
     7141 cctgcgggtc aataccgtct gcgggcaaaa tgttcgccac cggcacgctc aaatactcac
     7201 taaaaccgcc atcctgatgc acgccaatga ctgaaatttt ttcacagcaa ttggtacgcc
     7261 cgcttttaca cgccgggcat tgctgacagg caacataagg gatcacagca acttgctgac
     7321 catttttaag atcagcaata tttttaccca gcccaacaat ctccccacat atttcatggc
     7381 ctaaaacacg tggataacta aaaaatggtt gatttccacc ccaggcatga atatcggtac
     7441 cgcaaatccc gacagactta atttttatta atgcttcatt gtcacccgga ataggtatct
     7501 cgcgttgttt ccagactaat tctttcggct gctggcaaat taaaacattc atcgtagaca
     7561 tgattcggct ctccattttt tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg
     7621 aacggaaaat ctgttttgtg aaaacacacg cataaaaatg ttttaaatcg ggttttaatg
     7681 gactcacaag gaggaaaata tgagtcgttc acaaaattta cgccacaatg tgattaacca
     7741 ggtcattgat gatatggcgc ggggtcatat cccttcccca cttccatcgc aaagtgcgct
     7801 ggcggaaatg tacaacatca gccgcaccac agtgcgtcat attctcagcc acttacgcga
     7861 atgcggcgtc ttgacgcagg ttggaaacga ctatgttatt gcgcgtaaac ctgaccatga
     7921 tgacggtttc gcctgtacta ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc
     7981 ttttttcacg atgataaacc agcgccagtt acgcccaggg gaaacgtttt ctgaactgca
     8041 actggctcgg gccgcgggtg tcagcccggt tgtagtaaga gaatatcttt taaaattcgg
     8101 ccgttataat cttattcaca gcgaaaagcg cggccagtgg agcatgaagc aattcgatca
     8161 atcctacgcc gagcagctgt ttgagctacg cgaaatgctc gaaacacatt cacttcagca
     8221 ctttctcaac ctgcctgatc atgatccccg ctggctgcag gcgaaaacca tgctcgaacg
     8281 tcatcggcta ctgcgtgaca acatcggcaa tagctttcgc atgttctcgc aacttgacag
     8341 agattttcac tccttactgc tttcagctgc cgacaatatc ttttttgatc aatcgcttga
     8401 gatcatctca gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg
     8461 caatatcatt gccgtcgatg agcatatgac cattctcagc gctctgattt gccgcagtga
     8521 ccttgatgcc acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg
     8581 atccattaat gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt
     8641 agcgggtgca cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca
     8701 caatcagtcg actaaaaatg tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc
     8761 cgttaatgtc aaccttgcgt aattcacaat tacgtaaaca attaaaatta taaatacagg
     8821 agtccggcgt ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt
     8881 cgtcggcaga tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac
     8941 gcattcaaac caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc
     9001 gcagttcgct gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg
     9061 aaatcggcgc tttgctctcc gtgttttcac tcgcttacgg gattgcgcaa cttccttgcg
     9121 gcccactatt ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt
     9181 cactgttcca ggcaatgtct ggcatggtgc acaactttac gcagttcgtg ttggtgcgta
     9241 tcggtatggg gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact
     9301 ggttcaacat caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg
     9361 gcgttgccgt aagcccaccg attctggcgg cgatgatgct ggtgatgggc tggcgcggga
     9421 tgtttattac cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc
     9481 gcaaccgcga gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg
     9541 tcaatgcccg ccgagatccg ctcagttttg ccgaatggcg cagcctgttc cgtaaccgta
     9601 caatgtgggg aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg
     9661 cctggcttcc tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga
     9721 tggcggctat ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg
     9781 actggctggt caaaggggga atggctccga ttaaaagccg taagatctgc attattgccg
     9841 ggatgttctg ttctgccgcc tttacgctgg tcgtaccgca agcgacaaca tccatgacag
     9901 cggttctgct gattggcatg gcactgttct gtattcaaca tcctgctggg gcttgatcca
     9961 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
    10021 catctgcgcc tcttttgcgc cgatcattac tggttttatt gttgatacca cccactcatt
    10081 ccgtctggca ctaatcatct gcggttgcgt caccgcagcg ggggcactgg cgtacatctt
    10141 cctggttcgt cagccgatca acgacccacg taaagattaa tctccttatg cccgataaca
    10201 ttttgcttat cgggcatttt catggcgatt aaaatgtttc ccagttctcc tcgctatctg
    10261 ccacggccat tttacgcggc gcagctggcg tcacggtttt taccacagcc gatgtttcac
    10321 gctgctgttg ctgaatccgg aacactgcca ctgcttcggt caggcgactg gcctgctctt
    10381 ccagcgcggc ggcggcagcg gcagactctt ccaccagcgc ggcgttctgt tgagttaccc
    10441 ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc tcatcagaag
    10501 cagaagcaat ttcgcccata atgtccgtca cgcgggtcac ggcgctgaca atctccgcca
    10561 ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc accgagtctt
    10621 caatcaggct tttaatttca cgagccgcct gggcgctgcg ctgggccaga ttacgcactt
    10681 ctcccgcgac caccgcaaaa ccgcgccctt gctcacccgc acgcgcagcc tcaaccgccg
    10741 cgttcaaagc cagaatattg gtctggaagg caatgccgtc aattacgctg ataatatcgg
    10801 cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta tctaccactt
    10861 tgccgccgcg ttgcgccgtt tcagaagcac ttaacgccag atggctggcc tggcgcgcat
    10921 tttcggcgtt ctgcttcacc gttgcggtca gttactccat gctggctgcc gtctcttcca
    10981 gcgaagcggc ctgttgctcg gtgcgcgaag agagatcgtt attgccggtg gcgatttcgc
    11041 tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta cgcatcagct
    11101 ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag ccatccacct
    11161 caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc aggcgattca
    11221 ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc gccagtacga
    11281 cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg ttatcgctga
    11341 cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc ttctcgaaac
    11401 cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct gcacctaaca
    11461 gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg atctctgccg
    11521 ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc cagttttttt
    11581 ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt gaaccgctac
    11641 caatattgtt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg ttacgcgtct
    11701 gcaacaacgc gacccagctg ccattcagcg tggattgctg ctggcgaatg gtttgtaaaa
    11761 cagtgaaatt ttctttgtca ttctttaagg cattaaagaa cagaccgcct gatgtcagtt
    11821 gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg atacgtttta
    11881 acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag caaaaacttt
    11941 atgaaaaggt gtaacccacc atccatggaa aaaccaggcg ctggttatca gatactaaaa
    12001 agttattcat tatcgcccgg gatggcttct tgtcgggaat tgcccagagc aataaattcc
    12061 tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc aatctgccga
    12121 taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc tttggttagc
    12181 gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa accgtcgcgt
    12241 tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg atacgccaga
    12301 tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca ctgttgctca
    12361 gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc tcgcgcctgg
    12421 agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc aaaatcatta
    12481 atatagaaac agttaatcgt cacgttaccg ggactgcgct gcccctgcaa ttcctgattt
    12541 tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt atctttaaat
    12601 gtaaatagtt tgttaattag atcacattta catcacttac ttttgccaaa ctacgcaatc
    12661 gggaataaaa gcaatcatta acatattaat gattaagatg atcgaatccg aggagtggta
    12721 atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga tgcatggcag
    12781 gaagcgttcc agcaatcccc ctacaaagcc ccgcctaaaa ctgcggtctg gtttattaaa
    12841 ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg tgaaaaggta
    12901 ctgagcggtg cgactgttgc gctgattgtg ggaaaaacgg cgacgaaagt acgtgaagaa
    12961 gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct gccggaagag
    13021 agcttttacc gcccggcaat caaagcaaaa tgtcgtgatg gattctgccc cattggcgaa
    13081 accgtggctc tcagcaatgt cgataatctg accatctata ccgagatcaa cgggcgtcct
    13141 gccgatcact ggaataccgc cgatttacaa cgtaacgccg cgcagttgct gagcgccctg
    13201 agcgaatttg ccacgctgaa tccaggcgac gccattctgc tcggcacgcc acaggcgcgc
    13261 gtggaaatac agccaggcga tcgcgttcgt gttctcgcag aaggtttccc gccgctggaa
    13321 aatccggtag tggacgaacg tgaagtgacc acgcacaaga gcttcccaac gcagccacac
    13381 ccgcacggca cgctgtttgc cctcggcctg aactacgccg accacgccag cgaactggaa
    13441 tttaagccac cggaagaacc gctggtgttc ctgaaagcgc caaataccct cactggcgat
    13501 aaccagacct ccgtgcgtcc aaacaatatt gaatacatgc actatgaagc ggagctggtg
    13561 gtagttattg gcaaacaggc gcgtaacgtc agcgaagccg atgccatgga ttatgtcgcg
    13621 ggctacaccg tgtgtaacga ctacgccatt cgtgactatc tggaaaacta ctaccgccct
    13681 aacctgcggg taaaaagccg cgacggactg acgccgatgc tttcaaccat cgtgccgaaa
    13741 gaggcgatcc cggacccgca taatctgacc cttcgcacct tcgtcaacgg cgagttacgc
    13801 cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc ctacttaagt
    13861 gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacacccaa aggcttatct
    13921 gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg cctggtgaac
    13981 cgaattgtga gtgaggatac agcgaaatga aaaaagtaaa tcattggatc aacggcaaaa
    14041 atgttgcagg taacgactac ttccagacca ccaatccggc aacgggtgaa gtgctggcgg
    14101 atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg aaagaggcgt
    14161 tcccgaaatg ggccaatctg ccgatgaaag agcgtgcgcg cctgatgcgc cgtctgggcg
    14221 atctgatcga ccagaacgtg ccagagatcg ccgcgatgga aaccgcggac accggcctgc
    14281 cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc ccacaacttt gaatttttcg
    14341 cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag atgctcaact
    14401 acacgctggt gcagccggtg ggcgtttgtg cgctggtatc gccgtggaac gtaccgttta
    14461 tgaccgccac atggaaggtc gcgccgtgtc tggcgctggg caataccgcg gtactgaaaa
    14521 tgtcggaact ctccccgctg accgctgaca gcctgggtga gctggcgctg gaagccgata
    14581 ttccggcagg cgtgctgaac gtggtacagg gctacggcgc aaccgcaggg gatgcgctgg
    14641 ttcgtcatca tgacgtacgt gccgtgtcgt tcaccggcgg tacggccacc gggcgcaaca
    14701 tcatgaaaaa cgccgggctg aaaaaatact ccatggaact gggcggtaaa tcgccggtgc
    14761 tgatttttga agatgccgat attgaacgcg cgctggacgc cgccctgttc accatcttct
    14821 cgatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcaa agcatctacc
    14881 cggaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc gatccgaacg
    14941 atccgaatac ccaggttggc gcgcttatta gccagcagca ctgggaaaaa gtctccggct
    15001 atatccgtct cggcattgaa gaaggcgcaa ccctgctggc gggcggcccg gataaaccgt
    15061 ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaaccgtg ctggcagacg
    15121 ttgataaccg tatgcgagtc gcccaggaag agattttcgg gccggtcgcc tgcctgctgc
    15181 cgtttaaaga cgaagccgaa ggcttacgtc tggcaaacga cgtggagtac ggcctcgcgt
    15241 cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc attgaagctg
    15301 gcatggtgtt cgtcaacacc cagaacgtgc gtgacctgcg ccagccattt ggcggcgtaa
    15361 aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc gcggaaatga
    15421 agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc tgatatgggt
    15481 aagttagcgt gagccgccaa gatcacgcac gtaccgtcga tgtatcactc tgaactgcca
    15541 gggaaaaacc acggttgccg ccagggcgcg atcgacgggc ataaagagat cagcaagcgt
    15601 tgccgggaaa tgggcgtcga taccattatc gttttcgata cccactggct ggtcaacagt
    15661 gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa cgagctgccg
    15721 cattttatcc gcgatatgac ctacaactac gagggcaacc cggagttggg gcagcttatt
    15781 gccgatgaag ccttaaagct cggcgtgcgg gcaaaagcgc acaacattcc cagcctgaaa
    15841 ctggaatacg gcacgctggt gccgatgcgc tacatgaatg aagacaagca cttcaaagtg
    15901 gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa gctgggcgaa
    15961 gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc cagcggttcg
    16021 ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag ctacacccgc
    16081 gagttcgacc gccagatgga cgagcgcgtg gtgaagttgt ggcgcgaagg ccagttcaaa
    16141 gagttctgca atatgctgcc ggagtacgcc gactactgct acggcgaagg caatatgcac
    16201 gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa ggtggagttt
    16261 atcaccgagc tattcccaag ctctggcacc ggtcaggtta acgctgtttt cccgcttccc
    16321 gcgtaaggac gttttatgcc gcactttatc gttaaatgca gtgacaacat ccgcgaagaa
    16381 gccgacctgc cggggttgtt cgccaaagtg aatccgacgc tggcagccac gggtattttt
    16441 ccgctggcgg gtattcgcag ccgcgtgcat tgggtcgata cctggcagat ggccgacggg
    16501 cagcgtgatt atgccttcgt gcatatgacg ttgaaaatcg gcgcaggtcg cagcctggaa
    16561 agccgccagc aggcgggtga aatgctgttt gaactgatta aaacgcactt cgccgccctg
    16621 atggagagcc gcctgctggc gttgtcgttt gagattgaag agctgcatcc gacgctgaat
    16681 tttaaacaaa acaacgtgca cgcattgttt aagtagcgcg cagattgccc ggtggcgctg
    16741 cgcttaccgg gcctacaaaa ccccaaaccg tacaccgtag gccggataag gcgcagccgc
    16801 atccggcaat gccacaggat atcgctatgt tcgataaaca cacccacacc ctgatcgccc
    16861 agcgtctgga tcaggcagaa aaacagcgcg aacagatccg cgcgatctcg ctggattacc
    16921 cggagatcac catcgaagac gcttacgcgg tgcagcgtga atgggttcgc ctgaaaatcg
    16981 ccgaaggtcg cacgctgaaa ggccacaaaa tcggcctgac ctcgaaagcg atgcaggcaa
    17041 gctcgcagat cagcgaaccg gattacggcg cgctgctgga tgacatgttc ttccacgatg
    17101 gcagcgatat cccgaccgat cgctttatcg tgccgcgcat tgaagtggag ctggctttcg
    17161 tgctggcaaa accgctgcgt gggccaaact gcacgctgtt cgacgtctac aacgccacgg
    17221 actacgtgat cccggcgctg gagctgatcg acgctcgctg ccacaacatc gatccggaaa
    17281 cccagcgccc gcgcaaagtg ttcgacacca tttctgataa cgccgccaac gccggggtga
    17341 tcctcggcgg tcgtccgatc aaaccagacg aactggatct gcgttggatc tccgccctga
    17401 tgtatcgcaa tggcgtgatt gaagaaaccg gcgtcgccgc tggcgtgctg aatcatccgg
    17461 caaacggcgt ggcctggctg gcgaacaaac tcgcaccgta tgacgtacaa ctggaagccg
    17521 ggcaaatcat tctcggtggt tcgttcaccc gcccggttcc ggcgcgtaag ggcgacacct
    17581 tccacgtcga ttacggcaac atgggttcca ttagctgccg ctttgtttaa ggagagaacg
    17641 atggaaaaca gctttaaagc ggcgctgaaa gcaggccgtc cgcagatcgg attatggctg
    17701 gggctgagta gcagctacag cgcagagtta ctggccggag caggattcga ctggttgttg
    17761 atcgacggtg agcacacgcc gaataacgtg caaaccgtgc tcacccagct acaggcgatt
    17821 gcgccctacc ccagccagcc ggtagtacgt ccgtcgtgga acgatccggt gcaaatcaaa
    17881 caactgctgg acgtcggcac acaaaccttg ctggtgccga tggtacaaaa cgctgacgaa
    17941 gcccgtgaag cggtacgcgc cacccgttat ccccccgccg gtattcgcgg tgtgggcagt
    18001 gcgctggccc gcgcctcgcg ctggaatcgt attcctgatt acctgcaaaa agccaacgat
    18061 caaatgtgcg tgctggtgca gatcgaaacg cgtgaggcaa tgaagaactt accgcagatt
    18121 ctggacgtgg aaggcgtcga cggcgtgttt atcggcccgg cggatctgag cgccgatatg
    18181 ggttatgccg gtaatccgca gcacccggaa gtacaggccg ccattgagca ggcgatcgtg
    18241 cagatccgcg aagcgggcaa agcgccgggg atcctgatcg ccaatgagca actggcaaaa
    18301 cgctatctgg aactgggcgc gctgtttgtc gccgtcggcg ttgacaccac cctgctcgcc
    18361 cgcgccgccg aagcgctggc agcacggttt ggtgcgcagg ccaccgccgt gaagcccggc
    18421 gtgtattaat gccggatgcg ctgcgcttat ccggcctaca ctcgcaccaa accgtaggcc
    18481 ggataaggcg tttacgccgc atccggcaaa aagccgtacc aaatcgcgga ccggataagg
    18541 cgttacgccg catccgccat aaaccttgtc gtaccctaca aaaatcccat tagaggaaga
    18601 aaaatgagcg acacctcacc tgccataccg gagagtatcg atccggcgaa tcagcataaa
    18661 gcgctgactg ccggacaaca ggcggttatt aagaagctat ttcgccgcct gagcgtcttt
    18721 ctgttcgtgc tgtttatctt ctcgttcctt gatcgcatca acatcggctt tgccggactc
    18781 acgatgggac gcgacctcgg tctgagcgcc accatgtttg gcctcgctac caccctgttc
    18841 tatgccgctt atgtcatctt cggcattccc agcaacatta tgctgagtat tgtcggtgcg
    18901 cggcgctgga tcgccaccat catggtgctc tggggcatcg cctctactgc caccatgttt
    18961 gccactggcc ccaccagctt gtacgtactg cgtatactgg ttggcattac cgaagccggc
    19021 tttctgcctg gtattctgct gtatttaacc ttctggtttc cggcctattt ccgcgcccgt
    19081 gccaacgcct tgtttatggt agcaatgccg gtaacgacag cgttgggctc gatcgtttcc
    19141 ggctacattt tgtcgctgga tggcgtcatg gcattaaaag gctggcagtg gctgtttttg
    19201 ctggaaggct tcccgtcggt attactcggc gtcatggtgt ggttctggct tgatgactca
    19261 ccggacaaag ctaagtggct gacgaaagaa gacaaaaaat gcctgcaaga gatgatggat
    19321 aacgatcgtc tgacgctggt tcagccagag ggagccatca gccaccacgc catgcaacaa
    19381 cgcagtatgt ggcgggagat cttcactccg gtggtgatga tgtataccct ggcgtatttc
    19441 tgcctgacca acacacttag tgcgatcagc atctggacac cgcagatcct gcaaagtttt
    19501 aatcagggca gcagtaatat caccatcggc atgctggccg ccgtaccgca gatttgtacc
    19561 attctcggga tggtctactg gagccgtcac tcagatcgcc gccaggaacg aaggcatcac
    19621 accgcccttc cttatttgtt cgctgccgca ggttggttac tggcttcggc aactgatcac
    19681 aacatgatcc agatgctggg gatcattatg gcttcgaccg gatcattcag cgcaatggcg
    19741 attttctgga caacaccgga tcagtccatc agcctgcggg cacgagcgat cggtattgcg
    19801 gtgatcaacg ccactggcaa cattggttca gcgttaagtc cgtttatgat cggctggttg
    19861 aaagatttga ccggcagctt taacagtgga ttgtggtttg ttgccgcgct gctggtgatt
    19921 ggtgcgggga ttatctgggc gattccaatg cagtcctccc gtccgcgagc gaccctgtaa
    19981 ggaacgacga tgtgtgaccg tcagattgcc aatattgata tcagcaaaga gtacgatgaa
    20041 agcctgggca cggacgatgt gcattatcag tcgttcgccc gcatggcggc attttttggc
    20101 cgccatatgc tgccacatcg ccacgaacag tactttcaga tgcatttcct caatagcgga
    20161 cagattgagc tacagcttga cgatcatcgc tactcggtgg aagcgcccct gtttgtcctg
    20221 acgccgccgt ca
//
LOCUS       DAJTYZ010000077        20067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000077 DAJTYZ010000000
VERSION     DAJTYZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20067
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.077"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            49..558
                     /gene="radC"
                     /locus_tag="OO596_002588"
                     /pseudo
     CDS             49..558
                     /gene="radC"
                     /locus_tag="OO596_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            775..1011
                     /gene="rpmB"
                     /locus_tag="OO596_002589"
     CDS             775..1011
                     /gene="rpmB"
                     /locus_tag="OO596_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6718414.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1032..1199
                     /gene="rpmG"
                     /locus_tag="OO596_002590"
     CDS             1032..1199
                     /gene="rpmG"
                     /locus_tag="OO596_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6718415.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1297..2106
                     /gene="mutM"
                     /locus_tag="OO596_002591"
     CDS             1297..2106
                     /gene="mutM"
                     /locus_tag="OO596_002591"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6718416.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2145..2624)
                     /gene="coaD"
                     /locus_tag="OO596_002592"
     CDS             complement(2145..2624)
                     /gene="coaD"
                     /locus_tag="OO596_002592"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6718417.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2632..3909)
                     /gene="waaA"
                     /locus_tag="OO596_002593"
     CDS             complement(2632..3909)
                     /gene="waaA"
                     /locus_tag="OO596_002593"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6718418.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4322..5380
                     /gene="rfaQ"
                     /locus_tag="OO596_002594"
     CDS             4322..5380
                     /gene="rfaQ"
                     /locus_tag="OO596_002594"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6718419.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5377..6501
                     /locus_tag="OO596_002595"
     CDS             5377..6501
                     /locus_tag="OO596_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6718420.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6494..7291
                     /gene="rfaP"
                     /locus_tag="OO596_002596"
     CDS             6494..7291
                     /gene="rfaP"
                     /locus_tag="OO596_002596"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6718421.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7307..8323
                     /gene="waaO"
                     /locus_tag="OO596_002597"
                     /gene_synonym="rfaI"
     CDS             7307..8323
                     /gene="waaO"
                     /locus_tag="OO596_002597"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6718422.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8340..9335
                     /locus_tag="OO596_002598"
     CDS             8340..9335
                     /locus_tag="OO596_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR6718423.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9345..10037
                     /gene="rfaY"
                     /locus_tag="OO596_002599"
     CDS             9345..10037
                     /gene="rfaY"
                     /locus_tag="OO596_002599"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6718424.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10063..11088
                     /locus_tag="OO596_002600"
     CDS             10063..11088
                     /locus_tag="OO596_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCR6718425.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11173..12156
                     /locus_tag="OO596_002601"
     CDS             11173..12156
                     /locus_tag="OO596_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6718426.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12202..13455
                     /locus_tag="OO596_002602"
     CDS             12202..13455
                     /locus_tag="OO596_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6718427.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13525..14496)
                     /gene="rfaC"
                     /locus_tag="OO596_002603"
     CDS             complement(13525..14496)
                     /gene="rfaC"
                     /locus_tag="OO596_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6718428.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(14500..15546)
                     /gene="rfaF"
                     /locus_tag="OO596_002604"
     CDS             complement(14500..15546)
                     /gene="rfaF"
                     /locus_tag="OO596_002604"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6718429.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15556..16488)
                     /gene="rfaD"
                     /locus_tag="OO596_002605"
     CDS             complement(15556..16488)
                     /gene="rfaD"
                     /locus_tag="OO596_002605"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6718430.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16702..17898
                     /gene="kbl"
                     /locus_tag="OO596_002606"
     CDS             16702..17898
                     /gene="kbl"
                     /locus_tag="OO596_002606"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6718431.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17908..18933
                     /gene="tdh"
                     /locus_tag="OO596_002607"
     CDS             17908..18933
                     /gene="tdh"
                     /locus_tag="OO596_002607"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6718432.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTVICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            19172..>20067
                     /gene="waaH"
                     /locus_tag="OO596_002608"
     CDS             19172..>20067
                     /gene="waaH"
                     /locus_tag="OO596_002608"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR6718433.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         5419 a         4373 c         4736 g         5539 t
ORIGIN      
        1 aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc attacctggc
       61 ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt agcgaaattt
      121 gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg gatgcgtgaa
      181 gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca gctcacgggt
      241 gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt tataacgcat
      301 agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga aattatccgg
      361 gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc ttcgggttgt
      421 gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag ttgtcagttc
      481 atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt ttcttttgcc
      541 gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc tgttcgggac
      601 ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg ggtttggcat
      661 ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta aagcctgacg
      721 aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat agacatgtcc
      781 cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc ccacgcactg
      841 aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg ggttgagagc
      901 gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat cgataaaaaa
      961 ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta agtacttaga
     1021 ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct tctgctggta
     1081 ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa ctggaactga
     1141 aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa atcaaataat
     1201 tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat ctgcttgccc
     1261 ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc cgaagttgaa
     1321 accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca tgcggtggtg
     1381 cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag cgaccaacca
     1441 gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga gggctggatt
     1501 atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact tccccctgaa
     1561 aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta caccgatccg
     1621 cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt gctggctcat
     1681 cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca gaagtgcgcg
     1741 aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt aggggtaggg
     1801 aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg gctggcgtca
     1861 tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt gttgctgcgt
     1921 tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg taaaccgggc
     1981 tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg ggtgtgcggt
     2041 acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg gcagtgccag
     2101 aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc taacttcgcc
     2161 accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg atggcgcgcc
     2221 acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat cagaaacaca
     2281 ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc atattcaaaa
     2341 tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg ggcgaagttc
     2401 gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt tgcctgctgt
     2461 gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct ggcggcaatc
     2521 gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat atgaccattg
     2581 gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac ctcaatgcgt
     2641 tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc cctggttttg
     2701 atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt cggtgagtaa
     2761 agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc cgcttgcctg
     2821 ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca ataccggaat
     2881 agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg aaccgccaac
     2941 aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat cgccaaccac
     3001 aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc ttagtccagc
     3061 ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta ccaggatgag
     3121 caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca cactctcttc
     3181 accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact ggcggcgcag
     3241 cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca ggctaccggt
     3301 aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat cttcttcatt
     3361 ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac ccagtttggc
     3421 ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg gaattttacg
     3481 tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca acaccagttt
     3541 ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg gcagataaac
     3601 gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg gcgtcatggt
     3661 tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca acgggatcgc
     3721 cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt ttagcggatg
     3781 gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct tacgtccgcg
     3841 cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg tgtaaagcaa
     3901 ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta tttagctgtg
     3961 gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc aattcttgct
     4021 gaacatctat actttttttg tatttttatg cgattcattg atttctgctg tacgtgcagg
     4081 aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt tgattgattt
     4141 ctttattgaa aatcaacaat gtgcttaaaa agcattcggg tcggattgcc gcgaaagaag
     4201 tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg ttgagcctgg
     4261 ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa aagagttact
     4321 tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg gggatatgtt
     4381 attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa aaatcgatgt
     4441 gctgctttat caggacacca ccccgatcct gtctgaaaat ccagagatta acgcgctcta
     4501 cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt ttcatctcat
     4561 caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc aatggatggt
     4621 tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt atcatcatcg
     4681 gcagtctgct ttttggcgta aaagtttcac ccatttagtg ccgttgcagg gtggaaatgt
     4741 ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg tgaagcagac
     4801 aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg atcacgctgg
     4861 tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca aatgctggga
     4921 caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg aagtcgttct
     4981 gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc agggatgcca
     5041 gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg gtgcgttaat
     5101 cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg ccgctgcagt
     5161 taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc gtccttggtc
     5221 aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc gcgatcagcg
     5281 tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg ctgctgtcga
     5341 taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt ttgtttatat
     5401 aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca gacagtcgcc
     5461 gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg ccctgatgta
     5521 tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa tgcggagtat
     5581 tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt tggattcaac
     5641 aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga gaaagtcgcg
     5701 caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc cgcctttgag
     5761 cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga taagcaaatc
     5821 gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct gccaccgggg
     5881 atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat cttccgtaag
     5941 aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga cttcacgcgt
     6001 aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct gcgccacaac
     6061 acattgctat atgttgttgg acaggataag ccgcgaaaat ttgaggcgct ggcagaaaaa
     6121 cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc ggaattaatg
     6181 gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat tgtgctactg
     6241 gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta tgcgcattat
     6301 attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga aacattgaat
     6361 gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc ggaaaatgcg
     6421 cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc ggatatcata
     6481 acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc gcggtaaaga
     6541 tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg aaactcgccg
     6601 cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc gcggcacgac
     6661 cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag gcgcagaccg
     6721 cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt atggggtggc
     6781 atttggcgaa aaaggtatta atccgctgag cagaacctcg tttattatta ccgaagatct
     6841 gaccccaacc atcagtctgg aagattactg tgctgactgg gcgaccaacc caccagatgt
     6901 tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata tgcatgctgt
     6961 gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc ctttttccgg
     7021 taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc ttcgcacgcg
     7081 cgttccacgt cgttggcgcg ataaagatct tattgggctt tatttttctt cgatgaatat
     7141 cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgtcg ccccgcttaa
     7201 agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag ccacaaaaat
     7261 cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga gtgcccacta
     7321 ttttaatcca caagagatga tcaataagac aatcatcttc gatgaaaggc cagcggcgtc
     7381 agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt ttggttgtgg
     7441 tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt tccacgtttt
     7501 tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga aaagctatcg
     7561 tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac cgacgaccaa
     7621 aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta ttgatcaaca
     7681 agataagatc ctttacctgg atgctgatat cgcctgtcag ggaaacttaa agccgctgat
     7741 aacaatggat cttgccaata acgttgctgc tgttgttact gaacgcgatg ctaactggtg
     7801 gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact ttaattcagg
     7861 tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta aagcgatgtc
     7921 gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag atatacttaa
     7981 tcttatcctg tcagggaaag ttaaattcat tgatgctaaa tacaatactc aatttagttt
     8041 aaattatgaa ttaaaaaaat catttgtttg tccaattaat gatgaaaccg tattaattca
     8101 ttatgtcggc ccgacaaaac cctggcatta ctgggccggt tatccaagtg cgcaaccttt
     8161 tatcaacgcc aaagaagcat cgccctggaa aaatgaaccg tttatgcggc caattaattc
     8221 aaactatgct cgttattgcg ccaagcataa ttttaaacaa aatgaaccaa ttaacgggat
     8281 aatgaattat atttattatt tttatttaaa gataataaaa tgatattgag aaaagttaaa
     8341 tgaatgaatt tataaaagaa cggttttcgt atttagcaga taataaaaaa gaaaacgccc
     8401 cagaactaaa tgtttcctac ggtatcgata agaatttttt gtatggtgct ggcgtttcaa
     8461 tttcttccgt tttgattaat aattcagata ttaattttgt ctttcatgtt ttcactgatt
     8521 atgtggatga tgattattta aagtcattta atgaaacagc aaaacaattt aatacctcaa
     8581 ttattgtata tttaattgac ccaaaatact ttgctgatct gccgacgtca cagttttggt
     8641 cgtacgcgac atacttcagg gtattgtcct ttgaatatct gagtgaaagt atttccacac
     8701 tgctgtatct ggatgccgat gtcgtttgta aaggaagtct gaagcctctc acagaaatta
     8761 tatttaaaga tgagtttgct gcggtcattc ctgacaatgt tagtactcag gcggcatgtg
     8821 caaaacgcct caacattccc gagatgaatg gacgttattt caatgcaggc gttatctatg
     8881 tcaatattaa aaaatggcat gaagcaaatt tgacaccgta tttactcacg cttttacgag
     8941 gggaaactaa atatggctct cttaaatatt tagatcagga tgcgttgaat atcgcattta
     9001 atatgaataa tatctacctc gcgaaggatt ttgatactat ttataccctg aaaaacgaac
     9061 ttcatgatcg tagtcatcga aagtatcagc aaaccattac cgataaaaca gtattgattc
     9121 actatacagg gataactaaa ccatggcata gctgggctgg ttatccgtct gcatcatact
     9181 ttaatatcgc gcgtgaacaa tctccctgga agaaatatcc tcttaaagag gcgcggactg
     9241 ttgcagaaat gcagaaacaa tataagcatc tgtttgccca tggtgagtat attaaaggca
     9301 taacttcatt aattaagtac aagcttaaga aataaattac tcttatgatt acaagtatac
     9361 gctatcgcgg cttctcattt tattacaaag ataatgataa taaatataaa gaaatctttg
     9421 atgaaatact ggcctataat tttaaaacag taaaagtatt acgtaatatt aatgatacca
     9481 aagtatcatt gattgatacg aaatattgtc gctatgtttt taaagtcttt gccccgaaaa
     9541 cgaaaaggaa tgaacgtttt ttaaaatctt ttgtaaaagg tgactactat cagaatttga
     9601 tcgttgaaac cgatcgtgtc agaagtgcag gtcttacttt tcctaatgat ttttattttc
     9661 tggctgagcg taaaattttc aactatgcca gtgtttttat catgcttatc gagtatgtcg
     9721 agggcgtaga gctcaatgat atgccgatca ttccagaaga tgttaaagca gaaattaagg
     9781 cttcaatgga aaaattgcat gccttaaata tgctatcggg cgacccacac cggggtaact
     9841 ttatcgttag taaagatggt gtcaggataa ttgacctatc cggtaaaagc tgtacggcag
     9901 agcgtaaagc tcgtgatcgt ctggccatgg aacgtcacct gggtattgcc aacgaaataa
     9961 aagattatgg ctactattca gtaatctaca gaaccaaact acgcaaattt attaaaaaat
    10021 taaaaggcaa agcgtaaacc acacagtcaa aacggaacca acatggattt attagctgag
    10081 agtattactg aagtcgctgt ctctggggaa attgctaaca ccgatcgtgt gttaaatatc
    10141 gcttacggta ttgaccgtaa ctttttattt ggtgcggcag tatctatgca atcagttgtt
    10201 atgcataatc cggaccttgc ggttaagttt catctcttta ccgactacat tgatgaagat
    10261 tatctacaac gtgttaatgc ttttaccagc aaaaatgcta acgttgaagt aagaatttat
    10321 aaagtctcca gtgcctttat tgatatcttc cccagcctga aacagtggtc ttatgcaaca
    10381 ttcttccgtt tagttgcgtt ccagtatctg agtgaaacta ttgaaaatct gttatatatc
    10441 gatgctgatg tcatctgtaa aggctcatta gctggattgc ttgatattaa ttttgatggg
    10501 gataagtttg cagctgttat taaagatgtg ccttttatgc aggaaaaacc agcgaagcgt
    10561 ctggctatag agggacttcc agggaattat ttcaacgccg gtgtagtata tctgcagctt
    10621 gaagcatggg cgaaaaatga ttttatgaat aaagccattg ctatgctggc aagtgacccg
    10681 cagcacacga agtataaatg ccttgatcag gatattttaa atattctgtt ctttggtcat
    10741 tgtattttta ttagcggcga ttatgattgc ttttatggca tcgattatga gttaaaaaat
    10801 aaaagcgatg aagattataa aaagaccatt accgatgata ctaagctgat tcattatgtt
    10861 ggcgtaacga agccctggaa cgactggacg aattatccct gccagaagta ttttaatgag
    10921 gcttatcagg cttcttgctg gaatgatgtg gcgtttattc cagccacgaa tgaaaagcag
    10981 tatcaagtga aatatcaaca tgcaaagaaa aatggtgata cgtttaacgc ttttatttac
    11041 tttattaaat ttaaattaaa taagtataaa agaaaactat ttggataata ataaagatca
    11101 tggatgtctg agttacaata ataattaagg atgagtacta tcatatttta ttagttcagg
    11161 caagagaaca cgatgagcaa tgattaccct ttagtatcca taataatacc gacgtataat
    11221 tcatctgatt acattactga aactctaacg aaattagaaa aacaaactta cccaaatttt
    11281 gaaattgtta ttgttaatga tggttctaaa gataacacat caaacgtttt gagagagtat
    11341 gggttaaccc actctcgatt aattattatc aataaagaaa atggcggtgt ttcgtctgcc
    11401 aggaatacag gtatccgcaa ggcgcaagga cagtttatat gttttatgga tgatgatgat
    11461 gagatagatc ctaactatct gctgaagatg tataccagac aacatgagac gggaggggat
    11521 gcgatttatt gtgggcttta tggccattat ataaaagatg gtgttactta ctcacctata
    11581 aatacagagt ttaatgaagg atctttactt ttcgactttt tttataaaaa ggttagattc
    11641 catatagggt gcttgtttat aagaaaacaa cttctggaag agaataatct tttttttgat
    11701 gaagatttac gactaggaga agatctggat tttatctatc gactgctaat tacatgcgat
    11761 atgtatgcgg ttccatatta tatgtataag cataactata gagaaaattc cttaatgaac
    11821 tcatgtagaa ccatcactca ttatcgacat gagtcatttg cgcacgaaag aatctactct
    11881 tctgtgatgc agttatacaa aggtaacagg aaagaagaaa ttcatacatt attgagtcaa
    11941 aatagagctt atcataaaac tcgttattta tggaatgttc tacttaatgg tgattttgaa
    12001 ctattaaatc aattagttga aagcaatgaa aaagaattga atgattgtaa tctttctggc
    12061 aagagagata aaagacgagc aaaaatatta gcatcaaaaa attatattct ctggaggatg
    12121 gtaagactgg taaataggaa aaagaataaa cgttagatct tgcattaaat aatccgtcct
    12181 cgtagcatag gttgaaacta tatgtcattt tgttggaatg aaattaattc tggtataaag
    12241 tctttaattc tcatattatg tattttttct ttgatgactt tgtctttatg ggatgatgtt
    12301 gcaacaaagt ttcttcatgc agctggaatt atatctgcat tgtattttct aataacacca
    12361 aaaaaaacaa taactaacaa tcctactttg ttaattttca tctcattatg tcttttggga
    12421 atcataaata tcatctggta ttcacattat aaagtttcag gctctgttta taccaatgca
    12481 tatcgtggcc caatggaaac tgggaaaatt gccttgtgta gtgcttttat tttcttggtt
    12541 ctttttgcta aaaatgagtt gagaacaaaa ataaaatttg ggaaactaat tctgttcgca
    12601 tccctggcaa cgcagttact tttttttgcg catgccatgt ggcaacattt ctatttaaac
    12661 gttgaccgtg ttgcattatc agcttcccac gctacaacag caggctacat catccttttc
    12721 ccttctttac tggcatcaat tctcatttta aaatccgact ttagacataa aacaacatta
    12781 tatacaatta acttcatgct tagcttatgt gctgtcatag taactgaaac gcgtgcagcc
    12841 atattagtgt ttccattctt tgcgttacta ttaatcgtaa tggatagtta tattaataag
    12901 cgaattaatt ataagttata ttgttttatt gcgattgcat tattagcagg tgtattttct
    12961 tttaaagata cattgcttat gagaatgaat aacttaaata acgatttagt taattattcg
    13021 catgataaca ccagaacttc agtcggtgcc cgtctggcaa tgtatgaagt tggcttaaaa
    13081 acatattctc caataggaca atcactggaa aaacgagcgg aaaaaataca tgaactagaa
    13141 gaaaaagagc ctagattgag tggcgcttta ccctatatag attctcattt gcataacgat
    13201 ctcatagata cgttatcaac gcgtggtatt cctggagttg tattaacaat tttagccttt
    13261 tcagcaatac tcatatatgc cttaagaact gctaaagaac cttatatttt aatcttgctt
    13321 ttttcactac tggtagtagg cctaagtgat gtaatactct tttctaaacc ggttccgact
    13381 gctgtgtttg tcaccataat attgctttgt gcttatttta aagcacaatc agaccaatgt
    13441 ttattagata aataatgaat atgtgaaata aaatcagcaa ggaggctgat tttatttcaa
    13501 aattatctat taaggggttg taatttaaat catagcaacg ttttcttcaa tgaatcgctt
    13561 aacagcattt gctgtcaatt gagacaactc attccccgga gccctacaaa ccatctgatt
    13621 cttcccatat ccaccaatta atcccggatc ggttggtcca taaaccgtga tattgggtct
    13681 atccagtgcc gccgttaaat ggcttaaccc cgtatccacc gacactacaa atttagcccc
    13741 ggccagcacg cgggcaacgc cttccagact catcttcggc aatacttcaa cataagcaaa
    13801 tccttccgcc agtcgtttcg cccgctcttc ctcatgcggc gcgccccacg gaagtttaat
    13861 ccgtattcct gaatcagcca gtaaaccaat caattctcgc cagtgttctt ccggccagtg
    13921 tttatcatca cgggtcgtcg catgaagaaa tacggcatat tcgccagcat ctgtaggcag
    13981 gttcgtcagg aaatgctgtg caatagcata atcgccctgg gtttgcggtt tgctatagcc
    14041 cagacttttg gcaaacagtt cgcgggtgcg ttctacggcg tgctgctgtt ttgcaatatg
    14101 atgcttacga ttgtaaaaca gactggctaa aggttcgcga gcggtttgcc agtccatgcc
    14161 atgctttacg ccatgcgcca gacgcgtcac cagcgccgcg ctttttacca gcccctgagc
    14221 gtcgataact gcgtcatagt tctctgcttg tagcgcttca cgaaacgctt tgcgttccgc
    14281 ttttatgggg gccgagaacc aggctttacg ccagcgacgt attgccacag gaataactcg
    14341 ctcaacggcg gcgtgccagg aaggaatctg tgcgaaccct tcttccacca cccagtcaaa
    14401 cttaatccct gggattgcct gctgggcatc agtgagtgcg ggcaacgtat ggagaacatc
    14461 gcccatcgac gatgttttaa cgatcaaaac ccgcatccgt caggcttcct cttgtaacaa
    14521 tagcgcgttg agttcttcca gtacgcgctg gggagtaatg tcgattaagc tctggtgata
    14581 accctccgca gcgtcacctt tacgcacttt gtgatagccg gtaatcaggc ggatcacgcg
    14641 cgctttatgg gatagcggcg gtgtgaagtc cgggctactc ggaccataca gggcaaccag
    14701 cggacgattg agcgccgccg caacgtgcat caggccagaa tcgttagtga caatggcttt
    14761 acaggctgca atcaggataa ccgcttgatc aagctgtgtt tcccccgcca ggttccgaca
    14821 ccatgcctgc tgctcggtat tcaaagcggc aagaatctca ttgcccgctt catgatcttt
    14881 cgccgagcca aacagaacca cctgataacc ttcatcaatc agctgctttg ccagttccgc
    14941 atagtggtag tgtggccagc gttttgccgg accaaactcc gcacccgggc aaaaaccaat
    15001 catcggacgt tctgatgaaa gcgaaaattg attacaggta tatgattttt caccttcgct
    15061 cacctgcaat tgcggccata acaacggttt tggtagatct tgtgcggtac gcatcacgcc
    15121 tttgtcatag gccagcgcga catagcgttc caccattagc ggccaggctt ctttatcgag
    15181 cacgcgtaaa tcgttgagta aaccgtagcg catctcgccg cgccagccgg tgcgatgagg
    15241 aatacccgcg aagaaaggca ctaatgcaga tttgaaggag ttgggtaaga cgtaggcgcg
    15301 gtcgtagcgc ttttcacgca ggctatgacc cagtttgcgg cgttcgccga tttccagcgc
    15361 tccgtgaccg agaggcatag gaatagcttc gttaacttcc ggcatccgcg ataataatgg
    15421 acggcaccat gccggtgcca tcacatcgat tatcgcctgg ggatagcgcg cctggagcgt
    15481 gcgatagaga ctttgcgaca tcatcatgtc gccaacccaa gacgggccga tcaccagtat
    15541 tttcatgcag agctcttatg cgtcgcgatt cagccaggcc atgtattccg ttacaccttc
    15601 agcaacggtt ttgaacggtt tgtcgtaacc cgccgcgcgc agatttgtca gatctgcctg
    15661 agtgaacgcc tggtagcggc ctttcagctt atccgggaac ggaatgtatt cgatctggcc
    15721 tttcttgtga taagccagcg tagcatcagc tacagcctgg aaggattccg cacgaccagt
    15781 accgaggttg aagatgccgg aaacgccatt ttccaggaac cacagattca catcagccac
    15841 gtcgcccaca tagacgaagt cgcgtttgaa gttctcgcta ccttcaaaca gcttcggtga
    15901 ttcaccgttg ttaagctggg tgttgagatg gaaagcgacg ctcgccatgc tgcctttatg
    15961 gccttcacgc ggtccataaa cgttgaaata gcggaagcca acaatctgcg agttagcttc
    16021 aggcaggatt tggcgaacat attcatcaaa caggaatttt gagtaaccgt agacgttcaa
    16081 cggtttttcg tactcgcggg attcaataaa gtcggaggtg cgtccgccgt aggtggctgc
    16141 ggaagaggca tacaggaacg ggatttcgcg ctccagacag tagtgcagca gctctttgga
    16201 gtattgatag ttgttatcca tcatatactt gccatcccac tcggtggtgg aagagcacgc
    16261 gccttcgtgg aaaatcgctt cgacatcgcc gaactcttcg ccagccataa tctggatcag
    16321 gaagtcttcc ttatccatat agtccgcgat atccagatcc accaggttca caaacttggt
    16381 gccgtctttc aggttgtcca ccaccagaat atcggtgatg cctttatcat tcagggcctt
    16441 aacgatgttg ctgccgataa agcccgcgcc gccggtaacg atgatcataa ctgtaacctt
    16501 cgaattatgg agtcagagac aatctcagac acgaatactt ctatcatatc actacacggc
    16561 gttggcttca gccattcgcc aaaagtgaga ttgatggacg ttatttatcc tgaattttgc
    16621 agaagtgtta acgcgtcatc tcgtcgcgac ctataagttt gggtaatatg tgctggaatt
    16681 tgccctgtct ggagaatcgc aatgcgtgga gaattttatc agcagttaac caacgatctg
    16741 gaaaccgcac gggcggaagg gttgtttaaa gaagagcgca ttattacgtc tgcgcagcaa
    16801 gcagatatca ctgtggctga tggaagccac gtcattaact tttgtgccaa taactatctc
    16861 gggctggcga attatccgga tctgattgcg gcggcaaagg cgggaatgga ttctcacggt
    16921 ttcggcatgg cttcggtgcg ttttatttgc ggcactcagg acagccataa agagcttgaa
    16981 caaaaactgg cggcattcct ggggatggaa gatgcgattc tctactcttc ctgctttgat
    17041 gctaacggtg gcctgtttga aacgctgctg ggcgcggaag acgccattat ctccgacgca
    17101 ctgaaccacg cgtctattat tgatggtgtg cgtctgtgta aagctaaacg ctatcgctat
    17161 gccaacaacg atatgcagga gctggaagca cgtctgaaag aagcgcgtga agccggtgcg
    17221 cgtcatgtgc tgattgccac cgatggtgtg ttctcgatgg acggcgtgat tgccaacctg
    17281 aagggcgttt gcgatctcgc agataaatat gatgccctgg tgatggtaga cgactcccac
    17341 gcggtcggtt ttgtcggtga aaatggtcgt ggttcccatg aatactgcga tgtgatgggc
    17401 cgggtcgata ttatcaccgg cacgctcggt aaagcgctgg gcggggcttc tggtggttat
    17461 accgcggcgc gcaaagaagt ggttgagtgg ctgcgccagc gttctcgtcc gtacctgttt
    17521 tccaactcgc tggcaccggc cattgttgcc gcatccatca aagtactgga gatggtcgaa
    17581 gcgggtagcg agctgcgtga ccgtctgtgg gcgaacgcgc gtcagttccg tgagcaaatg
    17641 tcggcggcgg gctttaccct ggcgggagcc gatcacgcca ttattccggt catgcttggt
    17701 gatgcggtag tggcgcagaa atttgcccgt gagctgcaaa aagagggcat ttacgtcacc
    17761 ggtttcttct atccggtcgt tccgaaaggt caggcgcgta ttcgtaccca gatgtctgcg
    17821 gcgcataccc ctgagcaaat tacgcgtgca gtagaagcat tcacgcgtat tggtaaacaa
    17881 ctgggcgtta ttgcctgagg atgtgagatg aaagcgttat ccaaactgaa agcggaagag
    17941 ggcatctgga tgaccgacgt tcctgtaccg gaactcgggc ataacgatct gctgattaaa
    18001 atccgtaaaa cagtcatctg cgggactgac gttcacatct ataactggga tgagtggtcg
    18061 caaaaaacca tcccggtgcc gatggtcgtg ggccatgaat atgtcggtga agtggtaggt
    18121 attggtcagg aagtgaaggg cttcaagatt ggcgatcgcg tttctggcga aggccatatc
    18181 acctgcggtc actgtcgcaa ctgtcgtggc ggtcgtaccc atctgtgccg caacacgatc
    18241 ggcgtcggcg ttaaccgccc gggctgcttc gccgaatatc tggtgattcc ggcgttcaac
    18301 gccttcaaaa ttcccgacaa tatctccgac gacctggctt ccatttttga tcccttcggt
    18361 aacgccgtgc ataccgcgct gtcgttcgat ctggttggcg aggatgtgct ggtctctggt
    18421 gcaggcccga ttggtattat ggcagcggcg gtggcgaaac acgttggtgc acgcaatgtg
    18481 gtgatcactg atgttaacga atatcgtctc gaactggcgc gcaaaatggg tatcacccgt
    18541 gcggtcaacg tcgctaaaga aaaccttaat gatgtgatgg cagaactggg catggccgaa
    18601 ggctttgata tcggtctgga aatgtccggt gcgccgccag cgtttcgtac catgctcgac
    18661 accatgaacc acggcggtcg cattgcgatg ctgggtattc caccgtccga tatgtctatc
    18721 gactggacca aagtaatctt taaaggcttg ttcattaaag gtatttacgg tcgtgagatg
    18781 tttgaaacct ggtacaagat ggcggcgctg attcagtcag gtctggatct ctcgccgatc
    18841 attacccatc gtttctctat cgatgatttc cagaagggct ttgacgctat gcgttcgggc
    18901 cagtccggga aagtaattct gagctgggat taacacgaac aagggctggt attccagccc
    18961 ttttgcctga ggataatccg ttaaatatgt aaaatcctgt cagtgtaata aagatatcgt
    19021 aattgcgctg atctcttata tagctgctct cattatctct ctaccctgaa gtgactctct
    19081 cacctgtaaa aataatatct cacaggctta atagtttctt aatacaaagc ctgtaaaacg
    19141 tcaggataac ttcagaggtc gtcggtaatt tatgatgaac agcaccaata aacttagtgt
    19201 tattattccg ttatataatg cgggcgatga tttccgcact tgtatggaat ctttaattac
    19261 gcaaacctgg actgctctgg aaatcattat tattaacgat ggttcaacgg ataattctgt
    19321 tgaaatagca aagcattacg cagaaaacta tccgcacgtt cgtttgttgc atcaggcgaa
    19381 tgctggcgca tcggtggcgc gtaatcgtgg gattgaagtg gcaacgggca aatatgtcgc
    19441 ttttgtcgat gctgacgatg aagtctatcc caccatgtac gaaacgctga tgaccatggc
    19501 gttagaggac gacctcgacg tggcgcagtg caacgctgac tggtgttttc gtgaaacggg
    19561 agaaacctgg aaatccatcc ccagcgatcg ccttcgctca accggcgtat taaccggccc
    19621 ggactggctg cggatggggc tttcttcgcg ccgttggact cacgttgtct ggatgggggt
    19681 ttatcgccgc gatgttattg ttaaaaataa cattaaattt attgccggat tacatcatca
    19741 ggatattgtc tggacaacag aatttatgtt taatgcgctg cgtgcgcgat ataccgagca
    19801 atcgttatat aaatattatc tgcataatac gtcagtgagt cggttgcata gacaagggaa
    19861 caaaaacctt aattatcaac gtcactatat taagattact cgtctgctag agaaattaaa
    19921 tcgaaattat gccgacaaaa ttacgattta tccggaattt catcagcaaa taacttacga
    19981 agcattgcgt gtttgccatg cggtgcgcaa agagccggat attattaccc gccaacggat
    20041 gattgccgag atatttactt ccggtat
//
LOCUS       DAJTYZ010000078        19407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000078 DAJTYZ010000000
VERSION     DAJTYZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.078"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..245
                     /locus_tag="OO596_002609"
     CDS             <1..245
                     /locus_tag="OO596_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR6718434.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /locus_tag="OO596_002610"
     CDS             complement(296..1063)
                     /locus_tag="OO596_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6718435.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /locus_tag="OO596_002611"
     CDS             1259..2167
                     /locus_tag="OO596_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6718436.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /locus_tag="OO596_002612"
     CDS             2164..3426
                     /locus_tag="OO596_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6718437.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="OO596_002613"
     CDS             3423..4061
                     /locus_tag="OO596_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6718438.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="OO596_002614"
     CDS             4066..4842
                     /locus_tag="OO596_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6718439.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="OO596_002615"
     CDS             4931..6295
                     /locus_tag="OO596_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR6718440.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="OO596_002616"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="OO596_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6718441.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="OO596_002617"
     CDS             complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="OO596_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6718442.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="OO596_002618"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="OO596_002618"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6718443.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="OO596_002619"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="OO596_002619"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6718444.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="OO596_002620"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="OO596_002620"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6718445.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="OO596_002621"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="OO596_002621"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6718446.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="OO596_002622"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="OO596_002622"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6718447.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="OO596_002623"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="OO596_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6718448.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="OO596_002624"
     CDS             complement(12844..13167)
                     /locus_tag="OO596_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6718449.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="OO596_002625"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="OO596_002625"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6718450.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="OO596_002626"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="OO596_002626"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6718451.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="OO596_002627"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="OO596_002627"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6718452.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="OO596_002628"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="OO596_002628"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6718453.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="OO596_002629"
     CDS             complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="OO596_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR6718454.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="OO596_002630"
                     /pseudo
     CDS             complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="OO596_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="OO596_002631"
     CDS             complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="OO596_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6718455.1"
                     /translation="FFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICK
                     AGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQ
                     QLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
BASE COUNT         4592 a         5231 c         4847 g         4737 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17641 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17701 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17761 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17821 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    17881 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    17941 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18001 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18061 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18121 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18181 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18241 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18301 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18361 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18421 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18481 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18541 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18601 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18661 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18721 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18781 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18841 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    18901 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    18961 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19021 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19081 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19141 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19201 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19261 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19321 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19381 ggcgttcctg cgacaggaca aaaaagc
//
LOCUS       DAJTYZ010000079        19314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000079 DAJTYZ010000000
VERSION     DAJTYZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.079"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..444)
                     /locus_tag="OO596_002632"
     CDS             complement(<1..444)
                     /locus_tag="OO596_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718456.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVA"
     gene            complement(637..706)
                     /locus_tag="OO596_002633"
     tRNA            complement(637..706)
                     /locus_tag="OO596_002633"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(672..674),aa:Gly,seq:ccc)
     gene            complement(785..1540)
                     /locus_tag="OO596_002634"
     CDS             complement(785..1540)
                     /locus_tag="OO596_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6718457.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            1955..4252
                     /gene="xdhA"
                     /locus_tag="OO596_002635"
     CDS             1955..4252
                     /gene="xdhA"
                     /locus_tag="OO596_002635"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6718458.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4263..5141
                     /gene="xdhB"
                     /locus_tag="OO596_002636"
     CDS             4263..5141
                     /gene="xdhB"
                     /locus_tag="OO596_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6718459.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGSAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            5138..5617
                     /gene="xdhC"
                     /locus_tag="OO596_002637"
     CDS             5138..5617
                     /gene="xdhC"
                     /locus_tag="OO596_002637"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR6718460.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(5657..7435)
                     /locus_tag="OO596_002638"
     CDS             complement(5657..7435)
                     /locus_tag="OO596_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCR6718461.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            7914..9101
                     /gene="ygeW"
                     /locus_tag="OO596_002639"
     CDS             7914..9101
                     /gene="ygeW"
                     /locus_tag="OO596_002639"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR6718462.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9159..10355
                     /gene="dpaL"
                     /locus_tag="OO596_002640"
     CDS             9159..10355
                     /gene="dpaL"
                     /locus_tag="OO596_002640"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCR6718463.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            10413..11624
                     /locus_tag="OO596_002641"
     CDS             10413..11624
                     /locus_tag="OO596_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCR6718464.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            11677..13061
                     /gene="hyuA"
                     /locus_tag="OO596_002642"
                     /pseudo
     CDS             11677..13061
                     /gene="hyuA"
                     /locus_tag="OO596_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            13109..14041
                     /gene="arcC"
                     /locus_tag="OO596_002643"
     CDS             13109..14041
                     /gene="arcC"
                     /locus_tag="OO596_002643"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6718465.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(14082..15707)
                     /locus_tag="OO596_002644"
     CDS             complement(14082..15707)
                     /locus_tag="OO596_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCR6718466.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(15755..16525)
                     /gene="yqeC"
                     /locus_tag="OO596_002645"
     CDS             complement(15755..16525)
                     /gene="yqeC"
                     /locus_tag="OO596_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCR6718467.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            16628..17206
                     /gene="mocA"
                     /locus_tag="OO596_002646"
     CDS             16628..17206
                     /gene="mocA"
                     /locus_tag="OO596_002646"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCR6718468.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            17528..>19314
                     /gene="ygfK"
                     /locus_tag="OO596_002647"
     CDS             17528..>19314
                     /gene="ygfK"
                     /locus_tag="OO596_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCR6718469.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
BASE COUNT         5016 a         4994 c         4602 g         4702 t
ORIGIN      
        1 ggctacacta tttccctgat ctgatactaa tgccatatag ccccataaac tatccctcag
       61 ccagacagca tcatagtggg tttctgccgc tgttttgtca tcttcgaaag tagaagcaag
      121 aataaatgtt ccccagggtt tctccgtcaa cgttaacttt cgtgtgtttt ctgttaatgt
      181 taacaaccct tgaatttcgt ctttggtata gtggctcttt atttcatttt ttaatttggc
      241 tgagtgaatc agaagacgat aattagggta cttatctgga tttaacgata aataactttt
      301 atctgttgct gcccccgtgg aattaagaat gttaaataca gattcactcg tcacttctgc
      361 ccctgtcgcc tggcatgtgc cgcacgaaaa tataccagcg agcagaagag ttaatgcaga
      421 tttccctttc ataaaatatt ccatcaatcg tttgttaaaa gcgtaaataa ccacttattt
      481 aacaacttga aaataaggtg ttttacctgg gttgttacaa aaggattgca ttgcgtaaac
      541 gctttttatt tacaacaaaa tggggaaata ttacggcgat tgcgaaaagt ctgaagcgat
      601 tgaattaaga taatatagaa aatcagatac agcaaaagcg gcgaagggaa tcgaaccctc
      661 gtatagagct tgggaagctc tcgttctacc attgaactac gcccgcttcg agatgcgtaa
      721 ggcattataa accttacgct ctccttagca agtgccgcgc tgctgactgc tgattaattc
      781 gccatcagca ttttggcttg ctgccctgcg gcggcaagta acgtagcgga tcaattgccg
      841 ttgcacggta acgaatctgg aaatgcaggc gaacagatgc cgcatccgtg ctccccatag
      901 tggcgatttt ttgcccagcc ttcacgcttt gcccattatt taccagcatc gtgtcattat
      961 gggcgtaagc cgtaatgtaa tcttcactgt gtttaatcat gatgagatta ccgtagccac
     1021 gcagctggtt gcccacatac accacctttc ctgcacccgc ggcgtaaata ggtgtacccc
     1081 gtggagctga gatatcaatc cctttattgc cgccatctgc tgtcgaatac ggcatgataa
     1141 ctttccctgt cgttggccat aaccaacaac gttgccctac tggcggccag gatgatttcg
     1201 gtaccgctga tgacggtgta accgatgcgg ttttggtcgt tgatttggcg gttgatttac
     1261 gtgtactact gctacttttc gccccaccca gttttagttt ctgaccaact tcaatggtgt
     1321 aaggggggga aatgccgttc agtcgcgcca gttcttttac gctggttccc gtggtgcgcg
     1381 aaatacgata tagcgtatcc ccccgtttca cggtgtaaac ggagccggaa tacgttcctg
     1441 tatcggatga tttgctaccc gaacagcccg ccaaaagcag tccaaccgat aacaacatca
     1501 cgatacccag agatttttta ttcaggcgtc ccgcactcaa aatctttcct cgctaaaaaa
     1561 acaagacgcc ttatcatagc agccagcctc ttgataccca atctcttgct tctggaaaaa
     1621 cgtggtaata gagaaagagg ttaatttcat ttggcaaagg gaagtatcat cagcaaaata
     1681 ttcgctgtag aggtatcgaa cttcctgact ttttacccta tttcccatcc tgatgcgtaa
     1741 taatcccaat ttatcacatt gattttatcg ccactcatat tgatacgtat cactgcccct
     1801 attcagagtc ctgtttcgta tcatatctgt gtttttttaa cgtcatcaca cttcctacct
     1861 cccctaacgc ttatcgtcgt ttctggcgta aatcttgcct gcttagacta aatctttgcc
     1921 ataagaacca aatgttcaaa tcgaggggat ttctatggaa gcgcgggaag caaccgctac
     1981 gggtgaatca tgcatgcgcg tcgatgccat tgctaaggtc accgggcggg cacgatatac
     2041 tgacgattat gttatggcgg gcatgtgtta tgcgaaatat gtacgtagcc ctatcgcaca
     2101 tggttatgcc gtaagtatta atgatgaaca agccagaagt ttgccgggcg tactggcgat
     2161 ttttacctgg gaagatgtgc ctgatattcc attcgctaca gctgggcatg cctggacact
     2221 tgacgaaaac aagcgcgata ccgccgatcg cgcactgcta actcgccatg ttcgtcatca
     2281 tggtgacgcc gttgccatcg tcgtggcccg cgatgaactc acggcagaaa aagcggcgca
     2341 attggtcagc attgagtggc aagaattacc cgttatcacc acgccagaag cggcgctggc
     2401 agaagacgct gcaccaatcc ataacggtgg caatttactg aaacaaagca cgatgtcgac
     2461 gggtaatgtc caacaaacaa tcgatgccgc cgactaccag gtacaggggc actatcagac
     2521 ccccgttatt caacattgtc acatggaaag cgtaacatcg ctggcgtgga tggaggatga
     2581 ctcgcgaatt accatcgttt ccagcaccca gatcccgcac attgttcgcc gcgtggttgg
     2641 tcaggcgctg gatattccct ggtcatgcgt acgagtcatc aaaccgtttg tcggtggcgg
     2701 ttttggtaat aaacaggatg tactggaaga gccaatggca gcattcctga ccagcaagct
     2761 tggcggcatt ccggtgaaag tttcccttag ccgtgaagag tgtttcctcg caacccgtac
     2821 ccgccacgct tttaccattg acgggcaaat gggcgtgaac cgcgacggaa cattgaaagg
     2881 ttatagtctg gatgttctgt ctaacaccgg cgcttatgca tctcacgggc actccatcgc
     2941 ttctgcgggg aggaataaag tcgcttacct ttatcctcgt tgtgcctacg cttacagttc
     3001 aaagacctgc tataccaacc tcccctcggc tggtgcgatg cgtggttatg gcgcgccaca
     3061 agtcgtattt gccgttgagt ctatgcttga tgacgccgcg acagcgttag gtattgatcc
     3121 tgttgaaatt cgtttacgca acgccgcccg cgaaggagat gctaatccgc tcacgggcaa
     3181 acgtatttac agcgcagggt tgccggagtg tcttgaaaaa ggccggaaaa tctttgaatg
     3241 ggaaaaacgc cgtgcagaat gccagaacca gcaaggcaat ttgcgccgcg gcgttggcgt
     3301 cgcctgtttt agctatacct ctaacacctg gcctgtcggc gtagaaatag caggcgcgcg
     3361 ccttctgatg aatcaggatg gaaccatcaa cgtgcaaagc ggcgcgacgg aaatcggtca
     3421 gggtgccgac accgtcttct cgcaaatggt ggcagaaacc gtgggggttc cggtcagcga
     3481 cgttcgcgtt atttcaactc aagataccga cgttacgccg ttcgctcccg gcgcatttgc
     3541 ctcacgccag agctatgttg ccgcgcctgc gctgcgcagt gcggcactat tattaaaaga
     3601 gaaaatcatc gctcacgccg cagtcatgct acatcagtca gcgatgaatc tgaccctgat
     3661 aaaaggccat atcgtgctgg ttgaacgacc ggaagagccg ttaatgtcgt taaaagattt
     3721 ggcgatggac gctttctacc accctgaacg cggcgggcag ctctctgctg aaagctccat
     3781 caaaaccacc actaacccat cagcgtttgg ctgtaccttt gttgatctga cggtcgatat
     3841 tgcgctgtgc aaagtcacca tcaaccgcat cctcaacgtt catgattcag ggcatattct
     3901 taatccactg ctggcagaag gtcaggtaca cggcggaatg ggaatgggca ttggctgggc
     3961 gctatttgaa gagatgatca tcgatgctaa aagcggcgtg gtccgtaacc ccaatctgct
     4021 ggattacaaa atgccgacca tgccggatct gccacaactg gaaagcgcgt tcgtcgaaat
     4081 caatgagccg caatccgcat acggacataa gtcactgggt gagccaccaa taattcctgt
     4141 tgccgctgct attcgtaacg cggtgaagat ggctaccggt gttgcaatca atacactgcc
     4201 gctgacgcca aaacggttat atgaagagtt ccatctggca ggattgattt gaggataaca
     4261 tcatgtttga ttttgcttct taccatcgcg cagcaaccct tgccgatgcc atcaacctgc
     4321 tggctgacaa cccgcaggcc aaactgctcg ccgatggcac tgacgtactg attcagctcc
     4381 accatcacaa tgaccgttat cgccatattg ttgatattca taatctggcg gagctgcggg
     4441 gaattacgct ggcggaagat ggctcgctac gtatcggctc tgcaacgaca tttacccagc
     4501 taatagaagc tcctataact caacgtcatc tcccggcgtt atgtgctgcg gccacgtcca
     4561 ttgctggacc gcagatccgt aacgtcgcta cctacggtgg aaatatttgc aacggtgcca
     4621 ccagcgcaga ttctgccacg ccaacgctaa tttatgacgc gaaactggag atccactccc
     4681 cgcgcggtgt tcgtttcgtc ccgattaatg gctttcacac cgggccgggc aaagtgtctc
     4741 ttgagcatga cgaaatcctc gtcgcctttc attttccgcc acagccgaaa gaacacgcgg
     4801 gcagcgcgca ttttaaatat gccatgcgcg acgcaatgga tatttcaacg attggcagcg
     4861 ccgcacattg ccgactggat aacggcaatt tcagcgaatt acgcctggca tttggtgttg
     4921 ccgcgccaac gccgattcgc tgccaacatg ccgaacagac tgcacaaaat gcgccattaa
     4981 acctgcaaac gctggaagct atcagcgaat ctgtcctgca agatgtcgcc ccgcgttctt
     5041 catggcgggc cagtaaagag tttcgtctgc atctcatcca gacgatgacc aaaaaagtga
     5101 ttagcgaagc cgtcgccgcg gcggggggaa aattgcaatg aatcacagcg aaacaattac
     5161 catcgaatgc accattaacg ggatgccttt tcagcttcac gccgcaccag gcacgccgct
     5221 ctcggaatta ctccgcgaac aaggactgct aagtgtcaaa caagggtgct gcgtgggtga
     5281 atgtggtgcc tgtacggtgt tggtcgacgg cacagcaata gacagttgct tataccttgc
     5341 cgcctgggct gaaggaaagg agatccgcac gctggaaggt gaagcgaaag gcggaaaact
     5401 ttctcatgtt cagcaggctt atgcgaaatc cggcgcagtg cagtgcgggt tttgtacgcc
     5461 tggcctgatt atggctacca cggcaatgct ggcgaaacca cgcgagaagc cattaaccat
     5521 tacggaaatt cgtcgcggac tggcgggaaa tctttgtcgc tgcacggggt atcagatgat
     5581 tgtaaataca gttctggatt gcgagaaaac gaagtaaaag gatatccggc ctgaattcag
     5641 gccggattca ctgaggttat gtgtttaaca actcatattt cttaatcttg cgatagagcg
     5701 tagcaatgcc gatgcccagt tcatcagcaa cttgcttctt gctgttatga cgtgaaagcg
     5761 cctcgcggat catttgcttt tccatctcct ccagcgccgt gccgcccgca tcatcgagtg
     5821 acaggtgcgc ctcactgacc tctgttacat cactttgctc cgttgtgcca ttattcagca
     5881 gatttggcgg caatagcgtg ctgtcgataa cttcacctga aggaaccacg ttaaccagat
     5941 attccatcaa attgcttaac tcgcgcaggt ttccgggcca acgatgctta cgcaatattt
     6001 cgacgacatc gggagcaatg ccaggataaa ccgatcccag acgacgggta tgcagatgta
     6061 aaaagtaatg caccaatagt tcaatatctt cctgacgttc acgcagcggt ggcagagtta
     6121 tcgggataac attaagtcgg tagaagagat cttcgcggaa tttaccttcg gcaatgaact
     6181 gggccaaatt ctgattagtt gcagaaatga tgcgaatgtc gacttgtatt gggctactgg
     6241 caccaatcgg cagaatttca cgcgcctcaa tagcgcgcag taatttagcc tgcaacatta
     6301 atggcatatc acctatttca tcgagaaaca gcgtgcccgt attcgccgcc tgaatcaacc
     6361 ctgttttacc gttggcagaa gcaccagtaa atgcgccttt aacataaccg aacagttcgc
     6421 tttccagaag ctgctccgga atcgcggcac agttgatagc aataaagggt ttattccgtc
     6481 ttccgctcaa cttatggatt gctcgggcga cgacttcttt acccgtgccg ctttcaccaa
     6541 ccaccataac gctggatggg ctgggtgcaa tacggctaat gagtcgtttt aattgccgca
     6601 taacacggca ctcgccaacc aattgttcaa tatgtggttc atcaggtgca tttgctacag
     6661 aaaaactggt atgcgattgg tgaaatgcca ttaaaaataa ttgtcggccc tgaatgttat
     6721 gcaattgacc aatgattaat tcacttttat cgtcccacga aacaatatgc tgcatatgtc
     6781 catgagtaaa attactctca aatgttaatg gtctgaaacg gataggtttc ccaataatat
     6841 tattttgtac aacaccaagt gtttttaagg cagtctgatt aacaaactga acccgacttt
     6901 catcatcaac aactaatacg ccctgatcca tattatcgat catggtcgcg aatattttac
     6961 tgatgttatc tcctggcccc tgatcctcca gaagtttcga aacaaaaatg gtggatatat
     7021 ggcgaacata atcagaaaat tcgcgtaaat tatcactgat atgctcttgt tgctcgtggg
     7081 taacggcaat caaacttatc accccaacac aacgatcctg taaaatgaca ggcgtaccca
     7141 gaaatgcttt ttcgcggcaa ttttctttac tatcgcaacc ttcgcaaagg ggatcgaagc
     7201 gagactgtgt cacaactttt tcagttttcg tttccaggac gtggcggagc aggcgtgagt
     7261 tgccgctcaa ctggcgacca agaaacttcc catacgcgcc cgttccggcg acgcgacaca
     7321 agttttcatc aacgatctca acctcaaact gcaaaacgct ggcaagcatt ctggcaaaac
     7381 gctgaattgt cggttgaatt tgcatcaata ctgactgcgt agtagcaagc tccatagctt
     7441 taccttccag acttacttaa aagtcgatca ttgaagacgt tgatggttca cagatcatga
     7501 tgatattaac tcaggcgaaa ttggctttga taaaaacata agatttttat cattttctaa
     7561 tgcaattatg gaagagatat cacatttcta tatcaatatg agaattacgg cggtgagttt
     7621 atcaaactga agagagatag cctgcccctt tatcttattt ctgatactta gcagaaaata
     7681 aataacgcga taaaaaaagc caaacgtttt cgtattttac aaacaaccag aagctggcat
     7741 caatttgtga acaaccccac acattatccg tcaaattagt cttttgcagc cgcgcggata
     7801 attctggcac acttattgtt agtcccaggt atagctatga aaacaccaat cactttggca
     7861 agtcacagtg aaataaacca ctttgcctgt cattccacta ccgggactgt atgatgaaaa
     7921 ctgttaatga gctgattaag gatatcaatt cgctgacctc tcaccttcac gagaaagatt
     7981 ttttgttaac gtgggaacag acgccagatg aactgaaaca agtactggac gttgccgcag
     8041 cattaaaagc actgcgtgct gaaaacatct caaccaaagt ctttaatagt ggattaggta
     8101 tttccgtatt ccgcgacaac tccacccgta cccgcttctc ttatgcttcc gcgcttaacc
     8161 tgctcggcct tgcacaacaa gatctcgacg aaggcaaatc acaaatcgct cacggcgaaa
     8221 ccgtgcgtga aaccgccaat atgatctcct tctgcgccga cgctattggt attcgcgacg
     8281 atatgtatct gggcgcaggc aacgcctata tgcgtgaagt tggcgctgca cttgatgacg
     8341 gttacaagca gggggtactg ccacagcgtc cggctttagt gaacctgcaa tgcgatattg
     8401 accacccgac tcagtcaatg gctgacctcg cgtggttacg tgaacacttt ggttcactgg
     8461 aaaacctgaa aggtaaaaaa atcgccatga cctgggccta ctctccaagc tatggcaaac
     8521 cgctctctgt accacaaggc atcatcggtc tgatgactcg cttcggtatg gatgtcacct
     8581 tggcccatcc ggaaggctac gacctgatcc cggatgtggt tgaagtggcg aaaaacaatg
     8641 ctaaagcctc cggtggtagc ttccgtcagg tcaccagcat ggaagaagcc ttcaaagacg
     8701 cagacatcgt ttatccgaag tcatgggcac cttacaaagt gatggaagag cgtactgaat
     8761 tgctgcgtgc gaacgaccac gaaggcttaa aagcactgga aaaacagtgt ctggcacaga
     8821 acgcgcaaca caaagactgg cattgtactg aagagatgat ggaactgacc cgtgatggcg
     8881 aagccctgta catgcactgc ctgccagctg atatcagcgg cgtatcctgc aaagaaggtg
     8941 aagtgactga aggcgtattc gaaaaatacc gtatcgctac ctacaaagaa gccagctgga
     9001 agccttatat catcgccgcg atgatcctgt cccgtaaata cgccaaacca ggtgcactgc
     9061 tcgagcaact gctgaaagaa gcgcaagaac gcgtgaaata acccctcggg ccgatctgct
     9121 ggtcggccct ctatttccag aggccaaaag gataggatat gtccgttttc tcattgaaga
     9181 ttgatatcgc cgataacaaa tttttcaacg gcgaaacatc accgctcttt tcgcaaagcc
     9241 aggccaaact ggcgcgccag ttccaccaga aaatagctgg ttatcgccca acaccgcttt
     9301 gtgcgctgga cgatctcgca aacctttttg gtgtgaagaa aattctcgtc aaagacgaat
     9361 caaaacgatt cggtctgaac gccttcaaaa tgcttggcgg tgcgtacgcc atcgctcaat
     9421 tattgtgtga aaaatatcat cttgatatcg aaacgctgtc atttgagcac ctgaaaaatg
     9481 ccatcggcga aaaaatgact ttcgcgacca ccaccgacgg caaccacggg cgcggtgtgg
     9541 cgtgggcagc acagcaactc ggacagaatg cggtgattta catgccgaaa ggttctgctc
     9601 aggaacgcgt tgacgccatt ctgaacctcg gtgccgagtg catcgtcacg gatatgaact
     9661 atgacgatac cgttcgcctg accatgcaac acgcgcagca gcacggctgg gaagtggtac
     9721 aggacacggc atgggaaggt tacaccaaaa tcccaacctg gatcatgcaa ggctacgcaa
     9781 ccctggcaga tgaagccgtc gagcaaatgc gtgaaatggg cgtaaccccg acgcacgttc
     9841 tgctgcaagc cggtgtcgga gcaatggccg gtggtgtgct gggttatctg gtcgacgtct
     9901 acagcccgca aaatctgcac agcattattg ttgaacctga caaagctgac tgtatttatc
     9961 gctccggcgt caaaggcgac atcgtcaacg ttggcggtga tatggccacc atcatggcgg
    10021 gcctggcctg tggcgaacct aacccgctgg gctgggaaat cctacgtaac tgcgccaccc
    10081 aattcatctc ctgccaggac agcgttgccg cattaggtat gcgcgtgctg ggtaatccgt
    10141 acggcaacga cccgcgcatc atctccggtg aatccggcgc tgtcggtttg ggcgttctcg
    10201 cagcggttca ttatcacccg caacatcaaa gcctgatgga aaaactggcg ctggacaaag
    10261 atgccgtagt gctggttatc agcactgaag gcgacaccga cgtgaagcac taccgcgaag
    10321 ttgtctggga aggcaaacac gctgtagcac cttaatcacc ctattggaac tactccctgc
    10381 aaaacgggga gtaaaaaatc tggagaaaaa taatggctaa gaatattcca ttcaaactga
    10441 ttcttgaaaa agcaaaagat taccaggcgg acatgactcg cttcctgcgc gacatggttg
    10501 ctattcccag tgaaagctgc gacgaaaaac gcgtagtaca tcgtattaaa gaagagatgg
    10561 aaaaagtcgg cttcgataaa gttgaaatcg acccgatggg caacgttctc ggttatatcg
    10621 gccacggccc gcgtctggtg gcaatggacg ctcatatcga taccgtcggc attggcaaca
    10681 tcaaaaactg ggacttcgat ccgtacgaag gcatggaaac tgatgagcta atcggtggtc
    10741 gcggtacttc cgaccaggaa ggcggcatgg catctatggt ttatgccggt aaaatcatta
    10801 aagacctcgg tctggaagat gaatataccc tgctggttac cggtactgtg caggaagaag
    10861 actgcgacgg tctgtgctgg cagtacatta ttgaacaatc cggcattcgc ccggaatttg
    10921 tggtcagtac cgaaccaacc gactgccaga tataccgtgg tcagcgcggt cgtatggaaa
    10981 ttcgtattga tgttcagggt gttagctgcc acggttctgc gccagaacgc ggtgacaacg
    11041 ccattttcaa aatgggtccg attcttggcg aattacaaga actctcccaa cgtctgggtt
    11101 atgacgaatt cctcggcaaa ggcaccctca ccgtttctga aatcttcttc acatccccaa
    11161 gccgttgcgc tgtagcagat agctgcgccg tctctattga ccgccgtctg acctggggcg
    11221 aaacctggga aggcgcgctg gacgaaatcc gcgccctgcc tgcagtacag aaagctaacg
    11281 cggttgtttc tatgtacaac tacgaccgtc cgtcctggac tggcctggtt tacccaaccg
    11341 aatgctactt cccgacctgg aaagtggaag aagatcactt caccgttaaa gcactggtga
    11401 atgcctacga agggctgttt ggcaaagcgc cggttgttga taagtggacc ttctcaacta
    11461 acggcgtatc tatcatgggc cgtcacggca ttccggtgat cggctttggc ccgggtaaag
    11521 aacctgaagc gcatgcacct aacgaaaaaa cctggaaatc tcacctggtg acctgtgccg
    11581 cgatgtacgc tgcaatcccg ttaagctggc tggcaacaga gtaattactc ttcacttatt
    11641 cccctccggt tcgccggagg gtttttggag tttgctatgc gcgtattgat caaaaacggc
    11701 actgtcgtta acgcagatgg acaagccaaa caggatttgc tgattgaaag cgggattgtt
    11761 cgccagttgg gcaacaatat ttcgccgcag ctcccgtatg aagaaattga tgccactggc
    11821 tgttacgttt tccctggcgg cgtggatgtc catacgcatt tcaatattga tgtcggcatc
    11881 gcgcgcagtt gtgatgattt tttaccggta cccgcgcagc tgcgtgtggc ggtacaacaa
    11941 ccattattga ccatatggga tttggcccaa acggctgtcg gttacgccat caactggagg
    12001 tttatcgtgg ttatgccgcc cataaagcgg tcatcgatta cagctttcac ggtgtgatcc
    12061 agcacattaa tcacgcaatc ctcgacgaaa tcccgatgat ggtcgaggaa ggactgagca
    12121 gttttaaact ctatttaacc tatcaataca aactcaacga tgacgaggtt ttgcaggcat
    12181 tacgccgctt gcatgaatcc ggcgcgctga ccaccgtgca cccggaaaat gatgcggcta
    12241 tcgccagcaa gcgggcggaa tttatcgccg cagggttaac cgcgccgcgc tatcatgcct
    12301 tgagtcgccc tctggaatgc gaagcggaag ccatcgcccg catgattaac ctggcacaaa
    12361 ttgccggtaa cgccccgctc tatatcgtgc acctgtctaa cggcttaggt ctggattatc
    12421 tgcgtcttgc ccgtgcgaat caccagccag tctgggttga aacctgccca caatatctcc
    12481 tgttggacga acgcagttac gatacagaag atggcatgaa gttcattctt agcccaccgc
    12541 tgcgtaacgt acgcgagcag gacaaactgt ggtgtggcat cagcgatggt gcgattgacg
    12601 tggtggcgac cgatcactgc accttctcga tggctcaacg cctgcaaatt tctaaaggcg
    12661 atttcagtcg ctgcccaaat ggcttacccg gtgtggaaaa ccgcatgcag ttactgtttt
    12721 ccagtggcgt gatgacggga cgtataacac cggaacgctt tgttgaatta accagcgcaa
    12781 tgcccgccag gctgtttggc ctgtggccgc aaaaaggatt attagcgccc ggttccgatg
    12841 gcgacgtggt gattatcgac ccacgtcaga gccaacaaat tcagcatcgc catctccacg
    12901 acaacgccga ctactcgcca tgggagggtt ttacctgtca gggcgcgatt gtcagaacct
    12961 tatcccgtgg tgaaacgatt ttctgtgacg gcacctttac aggcaaagcc gggcgaggtc
    13021 gtttcctgcg acgcaaaccg tttgtccctc ccgtgctcta accactgtca gggatctata
    13081 cacaatcaag aatgatgagt gaggaaaaat gagtaagaaa attgttctcg ccctgggcgg
    13141 gaatgcgctg ggcgatgacc tggccggaca aatgaaagcg gtaaaaatta cttctcaggc
    13201 aattgttgat ttaattgccc aaggacatga agttatcgtc actcatggta acgggccgca
    13261 ggtgggcatg attaaccaag cctttgaagc tgccgcaaaa actgaagcac actcgccaat
    13321 gctgccgatg tctgtctgtg ttgccttaag ccagggttat attggttacg atctacaaaa
    13381 cgcgttaaga gaagaactgc tttctcgggg cattaataaa cctgtagcaa cgctggttac
    13441 tcaggtcgaa gtcgacgcta acgatccggc attcctcaac ccgaccaagc cgatcggttc
    13501 gttctttacc gagcaagaag cagagcaact gacaaaacag ggttacactc tgaaagaaga
    13561 tgctggtcgc ggctatcgcc gtgttgttgc ctcgccaaaa ccggttgata tcattgaaaa
    13621 agaaacggtt aaagctctgg tagatgcagg ccaggtggtg atcgccgttg gcggtggtgg
    13681 tatccctgtt attcgtgaag gtaaccatct gcgtggtgcc agcgcggtta tcgataaaga
    13741 ctgggccagc gcccgtttag cagaaatgat tgatgccgat atgctgatca ttctgactgc
    13801 ggtagaaaaa gtggccatta acttcggtaa agagaatgaa cagtggctcg atcgcctgtc
    13861 attaagtgat gctgaacgct ttatcgaaga aggtcatttt gcgaaaggtt ctatgctgcc
    13921 taaagtggaa gctgccgcct cgtttgctcg ctctcgcgca ggtcgtgaag cactgattac
    13981 cgtattgagc aaagcgaaag aagggattga aggcaaaacc ggaacggtga tttgtcagta
    14041 attttaaggc cgggtgggta ataccatccg gccattttca tttaagccac ttccagcact
    14101 tcttttgtcg ctttcacgcc ctgatgcatc aacatcatta acgcctcaag tacgccgccg
    14161 ccaatcgccc gggctttatc agaaacgctg gtgaaatcag cggtttcacc acgaggatcg
    14221 atatcaccaa ttttaaaacc accaaccact gccaggccgt cgttcaacaa gccacgcacc
    14281 atccccgtca acggtgcttt aatttcatgc tcaccaatcc aggcaatcac atcgccctct
    14341 ttcaccagat cgcctaattt cacgttggat cgcataatgc ctgcagcagg agcacggatc
    14401 acccgtcggg tggtatgccc cataatattg ccaggaacat cggtattctc ctgcgcacaa
    14461 ccagagtaaa tcacctgacc gagccagtgc ccgcgatttg tttcaattac cgcatgacaa
    14521 tccttccctg cagtaaagcc cggcccaagc gcgattgtta ctggtgccat atctgcccgc
    14581 gttcccaaat tctgtttcgc cagaatagcg tccaccacgc aaagcggttt cagttcatca
    14641 agcaatgaac aggcgggatc taccatcaca gggatgaatc cgcgttcggt aagtttcatc
    14701 gcttccgcag agctggttgc caggcgagca gtgacgcctt cgaccgtcat ttcgccatcg
    14761 aacacggcct gggcaaacgc cacggtacaa cgaatcactg tcggtttttc cacttccagc
    14821 atgatcactt taaaacccgc atgatacaga cgtagcgcca caccactggc gatatctccc
    14881 gcaccgcgaa tcaccaccag ctggtgtccg gaaggatgag aaggtttcat catcagccca
    14941 cccggcgcgt tatttttcac ccataatatt tctgccagca cgctgatggc gatctcctgc
    15001 ggcgtttccg cacctatgtt gtaaccaacg ggcgcatgta aacgggcaat atgttcttca
    15061 gccacgcctt tctcacgcaa ttgacgcagg aaaagctgaa ccttgcggcg acttgccagc
    15121 aaccccaacc atgcaatggg ctgttcaatg agtttgtcga gggcttcacg atcctgatta
    15181 ttcgtggcaa tcaggacaaa attatcaggg cgaatatcca gttcttccac tgccgcacca
    15241 aacgactcag catggagaag cgtggttgat ggtgggaata gttcaggatt gaggctttcg
    15301 cgataaatat cggcaacggc aatatcaaat cctaatagcg ccgcactctg ggctatcgcc
    15361 cggttgacat gccccgcgcc aatcaacacc agacgtggac gcataccatg gacgctgata
    15421 aatactgaca ttgcacctcc acaatctgac ccgacagcat ccgcaccgtt acgagccata
    15481 cgtccatgga ataatcgcgg cttacgttcc tgcaatgcct gaagtgactc ttcaatcacc
    15541 ttccgctcaa ccattccgcc accaattgta ccgacgatag aaccatcggc gcgcactaac
    15601 atttgtgcag aatggcgggg agtcgagcct cggctatcaa caatttgcgc catcgcaaac
    15661 ggacaatttt gctcttcgag ttttgcagcc tctgtgaaaa tattcatacc aacctcaatg
    15721 gatatccttt cagtaacccg gaatacccgg gccgctaatt cacaaatctg cgcgcgattg
    15781 caggatgttc ttgtatatcg cccagccaga ttgcctctac gtcgtgttgt tgcagcggtt
    15841 gaaggagctc gctttgtgca atcgcattct cacattgaga aaaacggtta atgaaccaga
    15901 ctcgccgaca accttgcggt acgtttttaa acgccccctg agggtggcga accagcgcaa
    15961 cgagatcgct cagttgcaag gttgcatcag gtgttaaccc ggtaatatca gcaaactgcg
    16021 accagcgatg gacattttct gtgctcactt tcgcgcccaa agtatgtcct cccatcacag
    16081 caatcacgca gcaactgctt ttgggtatgc aaggttcgtg ctcatcaggc gcttttaacg
    16141 gcattccacg cgagccatct gcttcaatga gaattacgtc acactctggt cgttgcacca
    16201 gtgcatcaat cgcttctggc gtaaatccct gcacttttcc ctggttcgct ttccagctgt
    16261 gaaaacaaaa tgaaatgggg gatgtaagag acgcatgagg aagcatggcg ggatcacgac
    16321 agaaaaccac gggccagtga gacgttggca taaacatatg tgtcgtcgtc gtgattaaca
    16381 cacgcctgcc gctagcctgg agcagttctg ccagccaaaa aagcaagctg gttttccccc
    16441 cagcaccaac aacagaaatc acagtgggac gtttctgcgc acctaaatca atgactaatg
    16501 ccgatgggtc aactatactt ttcacgagtc tttatgacct ctctgggata aattatcccc
    16561 aacttaatcc atcaggaagt aacgcaatta tcaggcgtta ttagccccta taaataatgg
    16621 aaccactatg tcagccatcg actgtataat taccgctgcc ggattatcat caagaatggg
    16681 gcaatggaaa atgatgttcc cctgggaaca gggaacaatt cttgatacaa gtatcaaaaa
    16741 tgcgttgcag ttttgtagcc gaattatttt agtcaccggc tatcgtggta atgaactgca
    16801 cgaacgttat gcgaaccaga gcaatatcac tattattcac aacccagatt atgcgcaggg
    16861 tttactaaca tccgtcaagg ccgcagtacc cgcagtgcaa acagaacatt gttttctcac
    16921 ccacggtgac atgccaaccc tcaccatcga tatttttaga aaaatctggt cgttacgaaa
    16981 tgatggcgca atactgccac tccataatgg catccccggc catccgattt tagtgtcaaa
    17041 accatgcctg atgcaggcaa tccagcgacc caatgtcacc aatatgcgtc aggcactcct
    17101 tatgggagat cattattccg tcgaaataga aaatgcagaa ataattttag atattgatac
    17161 tccggacgac tttattacag cgcaaaaaag gtatactaaa atttagaaaa tgtagttaaa
    17221 cgattgcgtt caaatattta atctctccag cactctttat cataatgata aggaatcact
    17281 gattttgaga aaagagaaaa agtgataaat gggctgacaa taataatttt caggagtgat
    17341 tttaattctc atttggcacc tgtgagttca ttcacaaaaa gcctttaagt ttcgagccac
    17401 ctcacatttt tttatatttc ccgccaaacc tggcaagagt ggtgcgattg ttgctctatc
    17461 cccctaaacc accggatttc tcaacaccgg tcactcaatg atatctgtat aagctaagga
    17521 gagggttatg ggggatatta tgcgtcccat tccgtttgag gaacttttga cgcgcatatt
    17581 tgatgaatac caacaacaac gctcaatctt tggtattccc gagcaacagt tttactcacc
    17641 tgtaaaaggt aaaactgtta gcgtcttcgg tgaaacctgt gccactcccg tcggccctgc
    17701 cgctggcccg cacacgcagc tcgcgcaaaa tattgtcact tcctggctga ctggcggacg
    17761 cttcatcgaa ctaaaaaccg tccaaattct tgaccgcctg gagctggaaa agccctgtat
    17821 tgatgccgaa gacgaatgct ttaacaccga atggtctacc gaatttacct tgcttaaagc
    17881 ctgggatgaa tacctcaaag cctggtttgc cctgcacctt ctcgaagcga tgttacagcc
    17941 ttctgattcc ggtaaatcgt tcatctttaa tatgagcatc ggttacaacc tcgaaggtat
    18001 taagcaaccg ccgatgcaac agttcatcga caatatgatg gacgcatctg accatccgaa
    18061 attcgctcaa tatcgcgata cgctgaataa attactccag gatgacgcat ttttagcccg
    18121 ccacggattg caggaaaaac gcgaaaactt gcaagcctta cccgctcgca tccccaccag
    18181 tatggtgcag ggcgtccccc tctccaccat gcacggctgt cctccgcatg aaatcgaagc
    18241 catttgccgc tacatgctgg aagaaaaagg gctcaacacc tttgtcaaac ttaacccgac
    18301 cttactgggg tacgcgcgtg ttcgtgagat cctcgatgtc tgcggtttcg gttacatagg
    18361 cttaaaagaa gagtcatttg atcacgacct caagctgacg caagcactgg aaatgctgga
    18421 gcgcctgatg gtactggcaa aagaaaaatc actcggcttt ggcgtaaaac tgactaacac
    18481 tctcggcacc atcaacaata aaggcgcact gcctggtgaa gagatgtata tgtcaggccg
    18541 tgcgctgttc ccgctctcca tcaatgttgc agcagttctc tctcgcgcct ttgacggcaa
    18601 actgcccatt tcttattccg gtggtgccag tcagctgact atccgcgata tttttgatac
    18661 aggtattcgc cctattacta tggcaaccga cctgctgaaa cctggcggct atctgcgctt
    18721 aagtgcctgc atgcgcgagc tggaaggctc cgacgcctgg ggacttgacc atgttgacgt
    18781 cgaacgactg aacagactgg cagcagacgc gttaaccatg gaatacaccc agaaacactg
    18841 gaagccagaa gagcgtattg aagtggcaga agacctgccg ctgaccgatt gctacgttgc
    18901 cccctgtgtt actgcctgcg ctatcaagca agatattccg gaatacatcc gtctgcttgg
    18961 cgaacaccgc tatgccgacg cgctggaact catctaccaa cgcaacgccc tacctgccat
    19021 taccggtcat atttgcgatc accagtgcca atacaactgt acccgcctgg attacgacag
    19081 tgcgctgaat atccgcgaac tgaaaaaagt cgcgctggaa aaaggttggg atgaatataa
    19141 gcaacgctgg cacaaacccg ccggttctgg ttcacgccat ccggttgccg tgattggtgc
    19201 aggtccggcg ggtctggcag caggttactt ccttgccaga gcgggccatc cggttacgct
    19261 gtttgaacgc gaagccaatg ctggcggcgt ggtgaaaaat atcattcctc agtt
//
LOCUS       DAJTYZ010000080        18831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000080 DAJTYZ010000000
VERSION     DAJTYZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18831
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.080"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(240..2111)
                     /gene="ppiD"
                     /locus_tag="OO596_002648"
     CDS             complement(240..2111)
                     /gene="ppiD"
                     /locus_tag="OO596_002648"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6718470.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2303..2575)
                     /gene="hupB"
                     /locus_tag="OO596_002649"
     CDS             complement(2303..2575)
                     /gene="hupB"
                     /locus_tag="OO596_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6718471.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2784..5138)
                     /gene="lon"
                     /locus_tag="OO596_002650"
     CDS             complement(2784..5138)
                     /gene="lon"
                     /locus_tag="OO596_002650"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6718472.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5326..6600)
                     /gene="clpX"
                     /locus_tag="OO596_002651"
     CDS             complement(5326..6600)
                     /gene="clpX"
                     /locus_tag="OO596_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6718473.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6726..7349)
                     /gene="clpP"
                     /locus_tag="OO596_002652"
     CDS             complement(6726..7349)
                     /gene="clpP"
                     /locus_tag="OO596_002652"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6718474.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7595..8893)
                     /gene="tig"
                     /locus_tag="OO596_002653"
     CDS             complement(7595..8893)
                     /gene="tig"
                     /locus_tag="OO596_002653"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6718475.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9237..9554)
                     /gene="bolA"
                     /locus_tag="OO596_002654"
     CDS             complement(9237..9554)
                     /gene="bolA"
                     /locus_tag="OO596_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6718476.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9859..10437
                     /locus_tag="OO596_002655"
     CDS             9859..10437
                     /locus_tag="OO596_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6718477.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10481..11956
                     /gene="ampG"
                     /locus_tag="OO596_002656"
     CDS             10481..11956
                     /gene="ampG"
                     /locus_tag="OO596_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6718478.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12417..13364
                     /gene="cyoA"
                     /locus_tag="OO596_002657"
     CDS             12417..13364
                     /gene="cyoA"
                     /locus_tag="OO596_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCR6718479.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13386..15377
                     /gene="cyoB"
                     /locus_tag="OO596_002658"
     CDS             13386..15377
                     /gene="cyoB"
                     /locus_tag="OO596_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR6718480.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15367..15981
                     /locus_tag="OO596_002659"
     CDS             15367..15981
                     /locus_tag="OO596_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6718481.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15981..16310
                     /locus_tag="OO596_002660"
     CDS             15981..16310
                     /locus_tag="OO596_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6718482.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16322..17212
                     /gene="cyoE"
                     /locus_tag="OO596_002661"
     CDS             16322..17212
                     /gene="cyoE"
                     /locus_tag="OO596_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6718483.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17397..17708
                     /locus_tag="OO596_002662"
     CDS             17397..17708
                     /locus_tag="OO596_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6718484.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17692..18234
                     /locus_tag="OO596_002663"
     CDS             17692..18234
                     /locus_tag="OO596_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6718485.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18290..>18831)
                     /locus_tag="OO596_002664"
     CDS             complement(18290..>18831)
                     /locus_tag="OO596_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6718486.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4217 a         4949 c         4576 g         5089 t
ORIGIN      
        1 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagggta
       61 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
      121 tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
      181 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
      241 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
      301 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
      361 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
      421 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
      481 gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc
      541 agaccggcag cctgcatagc ttccgcccct ttgccggctt tcaaatcaac cagcagtttc
      601 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
      661 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgat gcgcagcacg
      721 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccggcgc gccgttttca
      781 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
      841 aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
      901 ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag
      961 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
     1021 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
     1081 gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg
     1141 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
     1201 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc
     1261 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
     1321 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
     1381 tgcatcgttg cggcatccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
     1441 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
     1501 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
     1561 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacacc gttaatcagc
     1621 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
     1681 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
     1741 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
     1801 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
     1861 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatgcga
     1921 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
     1981 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
     2041 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
     2101 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
     2161 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac
     2221 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
     2281 acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
     2341 agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
     2401 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
     2461 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
     2521 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
     2581 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
     2641 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgccatc
     2701 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
     2761 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca
     2821 ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
     2881 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
     2941 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
     3001 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
     3061 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
     3121 gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
     3181 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
     3241 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
     3301 atggtcagca agtcaccgcc cacttccgtc cacgccagac cggttacctg accgacacgg
     3361 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
     3421 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
     3481 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
     3541 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
     3601 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
     3661 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
     3721 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
     3781 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
     3841 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcgggtt tttcacgccc
     3901 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
     3961 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
     4021 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
     4081 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
     4141 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
     4201 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
     4261 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
     4321 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
     4381 gcttcgtttt cgtccggcgc gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
     4441 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
     4501 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
     4561 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
     4621 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc
     4681 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
     4741 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
     4801 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
     4861 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
     4921 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
     4981 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
     5041 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
     5101 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
     5161 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
     5221 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
     5281 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
     5341 ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga
     5401 ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag
     5461 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
     5521 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
     5581 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
     5641 ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat
     5701 aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
     5761 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
     5821 gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
     5881 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
     5941 agctaccgta ccttcgatca gtttcaacag tgcctgctgt acgccttcac cggaaacgtc
     6001 tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
     6061 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
     6121 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
     6181 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
     6241 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
     6301 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
     6361 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
     6421 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
     6481 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
     6541 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
     6601 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
     6661 gcgcaagccg cttacagcgt tgtgccgccc tggataagta tagcggcaca gttgcgcctc
     6721 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
     6781 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
     6841 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
     6901 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
     6961 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca gcaagaaagc gcccatcgag
     7021 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
     7081 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
     7141 tctgggtttt ccgcttctag gaacagcatc tgcgccacaa tcaggttcgc catgtggtct
     7201 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
     7261 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
     7321 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaacct
     7381 gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt atgggtacgg
     7441 catcaccata tttcaagcat aacaatcttt tgttattacg ctaacaccga aagaggcttt
     7501 tcgcacggtt ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc ggtgacgggc
     7561 ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc atcagctcgt
     7621 tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca acagcctgtt
     7681 cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg tagaactcga
     7741 taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct ttcacgcgct
     7801 cttcgtcagc tttcagttcg ttggtgcgga taacttcgcc cagcagcagg ccaacaacta
     7861 cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct tgtttttcgt
     7921 tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg tcgatcagcg
     7981 cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga gacttaacgc
     8041 ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca gcgcgcagac
     8101 cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc agttccggca
     8161 gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta cctttcagat
     8221 tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg ccagctttgt
     8281 ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc gccagtacga
     8341 aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg aagtcgatgg
     8401 ttacgcggtc ttctgcttca acggcgccgt ctttttcttt ccaggtcgcc tgctgtttac
     8461 gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc ggtttttcaa
     8521 cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac tctacagagt
     8581 aggtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg ccagccggat
     8641 tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca cccagaacgt
     8701 cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact ttgcctttgc
     8761 ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta acagcggtct
     8821 cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga gtggtttcaa
     8881 ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc ttaccccgca
     8941 agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac attccctgtt
     9001 accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca tcacttttat
     9061 gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg ggcaattttc
     9121 gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc gcaggccgtt
     9181 tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat gcgtttttac
     9241 gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg caacccttcc
     9301 cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga gagttcctcc
     9361 gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt aaaacgatcg
     9421 ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg acgatagctt
     9481 tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc ttctatccgc
     9541 tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca tccctttaaa
     9601 tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg tttcgtcctg
     9661 gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag gcctcgcatc
     9721 agcgtttctt ttaccagggg tgattatttc gccaacatga tgaatttaca tgccttaccc
     9781 acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac acgcttaaga
     9841 gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc tctgtttatg
     9901 cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat tacactgcca
     9961 cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga tcagcgtacc
    10021 gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac cgcctcccga
    10081 gatctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg tggttacatg
    10141 gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta tgctgacgtg
    10201 tcccagggca acgtgcgcta caacatcgcg accaaagcgg atatcgccat catcgctacc
    10261 gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt tgaaggtgcg
    10321 ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac cgataccatc
    10381 gctgatatgt ctcaggacac cagcatccac gaattcatca agcagaacgc gcgttaattt
    10441 ctgccctctg gcccggtgca aaccgggcct gtagacgccc atgtccagtc aatatttacg
    10501 tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt ccgggctacc
    10561 gctcgccctg acatccggta ccttacaggc ctggatgacg gtcgagaata ttgatctcaa
    10621 aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc tctggtcacc
    10681 gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc tgctcgccac
    10741 gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaacctg gcacccaact
    10801 ccgctggatg gcggcactgg cggtggtgat cgctttttgc tcggcctccc aggatattgt
    10861 cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg gcgcggcaat
    10921 cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcttagccc tgtggctggc
    10981 agataaatgg ctgggctggc agggcatgta ctggttgatg gcggcgctgt tgatcccttg
    11041 tattatcgcg acgttgcttg caccagaacc gaccgacacc attcctgtgc caaaaacgct
    11101 ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg cctggcttat
    11161 tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc tgacaaccac
    11221 gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtta acaaaacgct
    11281 tggcttactg gcgaccattg tgggcgcatt gtacggtggg attttgatgc agcgcctgtc
    11341 gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg ctggttactg
    11401 gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct ttttcgaaaa
    11461 cctctgtggc gggatgggca catcagcctt tgtcgcactg ttaatgacgc tgtgtaataa
    11521 gtcattttcc gctactcaat ttgccctgct ctcagcgctt tccgctgtag gacgagttta
    11581 tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat tctatctatt
    11641 ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc agacgcttga
    11701 atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccgacag gttatgcctt
    11761 tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac tgctgttgac
    11821 gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg aagtcggggt
    11881 tttagtcgcc ctttctggcg tcgtgcttgg tggtttgctg gattatctgg ctctacgaaa
    11941 aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg ccgtaattat
    12001 tacggctaaa taaacatcag tatcgcttaa taatatttgt tcttttgtgc ggcttagcgt
    12061 ttggaaatta ttgacgcaat ttatgaattc tattttttct tacacgattc agctaatgag
    12121 tctttatttt ccccccaccc aattgtcacc ataatgataa ttatttgtta aacaattgtt
    12181 ttattcaaga ttggttatac caattgcccg tccaaactcc ttctgcactc ccctttcgtt
    12241 ataacgccct tttgcaacag cttcttaaaa tcaacttgat atgttttgca acatatgtaa
    12301 cctgacagcc aaatccaagt aacaggaatt taatcatgtt tacagtaatg taacctcccc
    12361 gtaaaatgcc cacacacttt aaacgccacc agatcccgtg gaattgaggt cgttaaatga
    12421 gactcaggaa atacaataaa agtttgggat ggttgtcatt atttgcaggc actgtattgc
    12481 tcagtggctg taattctgcg ctgttagatc ccaaaggaca gattggtctg gagcaacgtt
    12541 cactgatact gacggcattt ggcctgatgt tgattgtcgt tattcccgca atcttgatgg
    12601 ctgttggttt cgcctggaag tatcgtgcga gcaataaaga tgctaagtac agcccgaact
    12661 ggtcacactc caataaagtg gaagctgtgg tctggacggt acctatctta atcatcatct
    12721 tccttgcggt actgacctgg aaaaccactc acgctcttga gcctagcaag ccgctggcac
    12781 acgacgagaa gcccattacc atcgaagtgg tttccatgga ctggaaatgg ttcttcatct
    12841 acccggaaca gggcattgca accgtgaatg aaatcgcttt cccggcgaac actccggtgt
    12901 acttcaaagt gacctccaac tccgtgatga actccttctt cattccgcgt ctgggtagcc
    12961 agatttatgc catggccggt atgcagactc gcctgcatct gatcgccaac gaacccggta
    13021 cttatgacgg tatctccgcc agctacagcg ggccgggctt ctcaggcatg aagttcaaag
    13081 ctattgcaac accggatcgc gccgaattcg accaatgggt cgcaaaagcg aaacagtcgc
    13141 cgaacaccat gtctgacatg gctgcgttcg aaaaactggc cgcgcctagc gaatacaacc
    13201 aggtggaata tttctccaac gtgaaaccag acttgtttgc cgatgtgatt aacaagttta
    13261 tggctcacgg taagagcatg gacatgaccc agccagaagg tgagcacagt gcacacgaag
    13321 gtatggaagg catggacatg agccacgcgg aatccgccca ttaaaggggt tgaggaagaa
    13381 taaagatgtt cggaaaatta tcacttgatg cagtcccgtt ccatgaacct atcgtcatgg
    13441 ttacgatcgc tggcattatt ttgggaggtc tggcgctcgt tggcctgatc acttacttcg
    13501 gtaagtggac ctacctgtgg aaagagtggc tgacctccgt cgaccataaa cgcctcggta
    13561 tcatgtatat catcgtggcg attgtgatgt tgctgcgtgg ttttgctgac gccattatga
    13621 tgcgtagcca gcaggctctt gcctcggcgg gcgaagcggg cttcctgcca cctcaccact
    13681 acgatcagat cttcaccgcg cacggcgtga ttatgatctt cttcgtggcg atgcctttcg
    13741 ttatcggtct gatgaacctg gtggttccgc tgcagatcgg cgcgcgtgac gttgcgttcc
    13801 cgttcctcaa caacttaagc ttctggttta ctgttgttgg tgtgattctg gttaacgttt
    13861 ctctcggcgt gggcgaattt gcgcagaccg gctggctggc ctatccaccg ctatcgggaa
    13921 tagagtacag tccgggagtc ggtgtcgatt actggatatg gagtctccag ctatccggta
    13981 taggtacgac gcttaccggt atcaacttct tcgttaccat tctgaagatg cgcgcaccgg
    14041 gcatgaccat gttcaagatg ccagtattta cctgggcatc actgtgcgcg aacgtactga
    14101 ttattgcttc cttcccaatt ctgacggtta ccgtcgcgtt gttgaccctg gatcgctatc
    14161 tgggcaccca tttctttacc aacgatatgg gtggcaacat gatgatgtac atcaacctga
    14221 tttgggcctg gggccacccg gaagtttaca tcctgatcct gcctgttttc ggtgtgttct
    14281 ccgaaattgc ggcaaccttc tcgcgtaaac gtctgtttgg ttatacctcg ctggtatggg
    14341 caaccgtctg tatcaccgtg ctgtcgttca tcgtttggct gcaccacttc tttacgatgg
    14401 gtgcgggcgc gaacgtaaac gccttctttg gtatcaccac aatgattatc gccatcccga
    14461 ccggggtgaa gatcttcaac tggctgttca ccatgtatca gggccgcatc gtgttccatt
    14521 ctgcgatgct gtggaccatc ggttttatcg tcactttctc ggtgggcggg atgactggcg
    14581 tgctgctggc agtaccgggc gcggacttcg ttctgcataa cagcctgttc ctgattgcgc
    14641 acttccataa cgttatcatc ggcggcgtgg tcttcggctg cttcgcaggg atgacctact
    14701 ggtggcctaa agcgttcggt ttcaaactga atgaaacctg gggtaaacgc gcgttctggt
    14761 tctggatcat cggcttcttc gttgccttta tgccgctgta tgcgctgggc ttcatgggca
    14821 tgacccgtcg tttgagccag cagattgacc cgcagttcca caccatgctg atgattgcag
    14881 ctagcggtgc agtactgatt gcgctgggta ttctctgcct cgttattcag atgtacgttt
    14941 ctattcgcga ccgcgaccag aaccgtgacc tgactggcga cccgtggggt ggccgtacgc
    15001 tggagtgggc aacctcttcc ccgcctccgt tctataactt tgccgttgtg ccgcacgttc
    15061 acgagcgtga tgcattctgg gaaatgaaag agaaaggcga agcgtataaa aagcctgacc
    15121 actatgaaga aattcatatg ccgaaaaaca gcggtgcagg tatcgtcatt gcagctttct
    15181 ccaccatctt cggtttcgcc atgatctggc atatctggtg gctggcgatt gttggcttcg
    15241 caggcatgat catcacctgg atcgtgaaaa gcttcgacga ggacgtggat tactacgtgc
    15301 cggtggcaga aatcgaaaaa ctggaaaacc agcatttcga tgagattact aaggcagggc
    15361 tgaaaaatgg caactgatac tttgacgcac gcgactgccc acgcgcacga acacgggcac
    15421 cacgatgcag gcggaaccaa aatcttcgga ttttggatct acctgatgag cgactgcatt
    15481 ctgttctcaa tcttgtttgc tacctatgcc gttctggtga acggcaccgc aggcggcccg
    15541 acaggtaagg acattttcga actgccgttc gttctggttg aaactttctt gctgttgttc
    15601 agctccatca cctacggcat ggcggctatc gccatgtaca aaaacaacaa aagccaggtt
    15661 atctcctggc tggcgttgac ctggttgttt ggtgccggat ttatcgggat ggaaatctac
    15721 gaattccatc acctgattgt taacggcatg ggtccggatc gcagcggctt cctgtcagcg
    15781 ttctttgcgc tggtcggcac gcacggtctg cacgtcactt ccggtcttat ctggatggcg
    15841 gtgctgatgg tacaaatcgc tcgtcgcggc ctgaccagca ctaaccgtac ccgcatcatg
    15901 tgcctgagcc tgttctggca cttcctggat gtggtttgga tctgtgtgtt cactgttgtt
    15961 tatctgatgg gggcgatgta atgagtcatt ctaccgatca cagcggcgcg tcccatggca
    16021 gcgtaaaaac ctacatgaca ggctttatcc tgtcgatcat tctgacggtg attccgttct
    16081 ggatggtgat gacaggagct gcctctccgg ccgtaattct gggaacaatc ctggcaatgg
    16141 cagtggtaca ggttctggtg catctggtgt gcttcctgca catgaatacc aaatcagatg
    16201 aaggctggaa catgacggcg tttgtcttca ccgtgctaat catcgctatc ctggttgtag
    16261 gctccatctg gattatgtgg aacctcaact acaacatgat gatgcactaa gagcggcggt
    16321 tatgatgttt aagcaatacc tgcaagtaac gaaaccaggc atcatctttg gcaacctgat
    16381 ctcggtgatt gggggattcc tgctggcctc aaagggcagc attgattatc ccctgtttat
    16441 ctacacgctg gttggggtgt cactggttgt ggcgtcgggt tgtgtgttta acaactacat
    16501 cgacagggat atcgacagaa agatggaaag gacgaagaat cgggtgctgg ttaaaggcct
    16561 gatctctcct gctgtctcgc tggtgtacgc caccttgcta ggctttgctg gctttatgct
    16621 gctgtggttt ggcgcgaatc cgctggcctg ctggctgggg gtgatgggct ttgtggttta
    16681 tgtcggcgtt tatagcctgt acatgaaacg ccactcggtc tacggcacat tgattggttc
    16741 gctctccggc gctgcgccgc cggtgattgg ctactgtgca gtaaccggcg agttcgatag
    16801 cggcgcagcg atcctgctgg ctatcttcag cctgtggcag atgcctcact cctatgccat
    16861 cgccattttc cgctttaagg attatcaggc ggcaaacatt ccggtattgc cagtggtaaa
    16921 aggcatttcg gtggcgaaga atcacatcac gctgtatatc atcgcctttg ccgttgccac
    16981 gctgatgctc tctcttggcg gttacgctgg gtataaatat ctggtggtcg ccgcggcggt
    17041 gagcgtctgg tggttaggta tggccctgcg cggttataaa gttgctgatg acaggatctg
    17101 ggcgcgcaag ctgttcggct tctctatcat cgccatcaca gccctctcgg tgatgatgtc
    17161 cgttgatttt atggtgccgg actcgcatac gctgctggct gctgtttggt aacacaacct
    17221 ctctattaaa aaggtgctac ggcacctttt ttattgtctg agatcagaga tacggctaat
    17281 aaccgtatgt cttgacagag tgagcagatg aacataaatt aatcatacgg ttgatagccg
    17341 tattatgtat gagatcgact ccctacgatt tcattcagtt aatcaaagag gtcataatgc
    17401 ctggaaaaac agccactcta cccgatgtcg ataaaacgct gaaaaatgcg cgggtcgaac
    17461 tcaaaactag tcccgacgcc aaaaacaaac ttcgtgaagc tgcgcaagcc gttggggtag
    17521 atttaagcgc ttttattttg agcgctgcta tggaacgcgc cgaaagtgtg cttgataacc
    17581 aacgtcgccg tgagctttcg aatcaaagct gggaactgat gaaccaactc atcgctgaac
    17641 ctgctcaacc gacgctcgcc ctcaaggcgt taatgaaaag gaaaaacagc gatggtcgac
    17701 aagcatgaag agattactct gcccatagtc ctctcctgta attatcagtc tgatattact
    17761 tatcctgggc aaaaacagtt tgattgcggt aaccctgtta tcgataaatt tgtacgcgca
    17821 tcgctaaaga aaagtgtgcg taatagcgac tgtgcggcta aagcacttat tgacagacaa
    17881 agtggtgaac tgatcggcat ctgtactttt acggcatatt cgctggaaaa acaacgcgtt
    17941 tctggcgtcc ttcagggttc acaaccttca gaaattggtg ttgtcagatt agtcatgttg
    18001 ggggtagcac ggaagtatca aaagcggggc tttggtcagg acctactatg tgattttttt
    18061 gaacatgtaa aaataattca ccaggcatta ccaattaaag gggtttatct tgatgctgac
    18121 cctgccgcca ttaattttta tgctcgtctc ggctttgttc agctttcagc gacaccaaat
    18181 gcttttggtg ctgtacctat gtttttggcg attcagcata ttctcgcggc ttagattatt
    18241 atcgccaccc gccagggcaa gttttgcctt agccgggggc tgagcagcgt taccactttt
    18301 gttcaattgc ccccgctttc agcaactcca catccttgcc gttaatggtt gccgttgcgg
    18361 caacgattgt ggtccttaca gttgcacaag attttcgctc aaattccggt ttataaagaa
    18421 taagagacga attactggta aatgaaccaa ataggtaatc agtaaagcta atattcagcc
    18481 ggtttaaacc gaaaggcgcg aagccaaccg tttggttaat ctccttaccg gatgggcgac
    18541 gaatgctgaa tgtcacgcga agattggcca cttcgcctaa tttattggtg gtggcgaaga
    18601 ggctatccat gcaggaaaaa cctttatcta aaaaattatc tcgcatccca gaacgggtaa
    18661 ccgtaatcac gggtaacgta tcgtcaaccg tttttctgtc aatcatgtct tttcgacaag
    18721 tagccagaaa gtcttccggg ccaccataaa tgtggacacc tttatcaaga tattccttcg
    18781 ctttcttaca atctacctca acatactgac cattggcgta cataatgccc a
//
LOCUS       DAJTYZ010000081        18525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000081 DAJTYZ010000000
VERSION     DAJTYZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.081"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1712
                     /locus_tag="OO596_002665"
     CDS             <1..1712
                     /locus_tag="OO596_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6718487.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1713..3362
                     /locus_tag="OO596_002666"
     CDS             1713..3362
                     /locus_tag="OO596_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6718488.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3367..4143
                     /locus_tag="OO596_002667"
     CDS             3367..4143
                     /locus_tag="OO596_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6718489.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4217..5401)
                     /gene="atoB"
                     /locus_tag="OO596_002668"
     CDS             complement(4217..5401)
                     /gene="atoB"
                     /locus_tag="OO596_002668"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCR6718490.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5432..6754)
                     /locus_tag="OO596_002669"
     CDS             complement(5432..6754)
                     /locus_tag="OO596_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCR6718491.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6751..7401)
                     /gene="atoA"
                     /locus_tag="OO596_002670"
     CDS             complement(6751..7401)
                     /gene="atoA"
                     /locus_tag="OO596_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HCR6718492.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7401..8063)
                     /gene="atoD"
                     /locus_tag="OO596_002671"
     CDS             complement(7401..8063)
                     /gene="atoD"
                     /locus_tag="OO596_002671"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCR6718493.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8259..9644)
                     /gene="atoC"
                     /locus_tag="OO596_002672"
     CDS             complement(8259..9644)
                     /gene="atoC"
                     /locus_tag="OO596_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCR6718494.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9641..11467)
                     /gene="atoS"
                     /locus_tag="OO596_002673"
     CDS             complement(9641..11467)
                     /gene="atoS"
                     /locus_tag="OO596_002673"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCR6718495.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11634..14483
                     /gene="rcsC"
                     /locus_tag="OO596_002674"
     CDS             11634..14483
                     /gene="rcsC"
                     /locus_tag="OO596_002674"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6718496.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14683..15333)
                     /gene="rcsB"
                     /locus_tag="OO596_002675"
     CDS             complement(14683..15333)
                     /gene="rcsB"
                     /locus_tag="OO596_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6718497.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15350..18022)
                     /gene="rcsD"
                     /locus_tag="OO596_002676"
     CDS             complement(15350..18022)
                     /gene="rcsD"
                     /locus_tag="OO596_002676"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6718498.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4536 c         4867 g         4596 t
ORIGIN      
        1 ctgagatgcg ccagacgctg acgctgcaca aaggggcgaa ttatatttcg ctggcgcaga
       61 acattcagca atctggcttg ttaagcgcag aactgcaaca aaatgggcaa gtgcaggaca
      121 gcattagcac aaaactgtct tttgtggata acagctggcc cgttgaacag cagaaaaatg
      181 tcatgctcgg cggtggcgag aacgcgctga tgttgcccga gcaggctagc aatatccggc
      241 tacaaagtag cgaaacgccg caggagattt tccgcaacaa tcttgatgcg ttagtcgatg
      301 gaccatgggg tggcgtgatc aacaccggta gccgtctgat cccgctcagt ctcgcctggc
      361 gttcgcttgc cgatcatcaa agtgccgccg ctaacgacat tcgtcagatg attcaggata
      421 accgtctgcg gctgatgcaa ctggcggggc ccggagcgcg ctttacctgg tggggtgaag
      481 atggcaatgg tgacgccttc cttacggcat gggcatggta cgccgactgg caggccagcc
      541 aggcgctcgg cgtaacgcaa caaccggaat actggcagca tatgctcgac agttatgccg
      601 agcaggcgga taacatgccg ttattgcatc gggcgctggt gctggcgtgg gcacaggaga
      661 tgaatctgcc gtgcaaaacg ttgttgaaag ggttggatga agctatcgcc cggcgcggaa
      721 ctaaagatga agatttctct gaggaagaca tccgcgatat caatgacagc ctgatcctcg
      781 atacaccgga atctccactg gcagatgcgg tggcaaacgt cttaaccatg acgttgctga
      841 aaaaagcgca gttgaagtcc acggtgatgc cacaggttca gcaatatgcg tgggataaag
      901 cggcaaacag caatcagccg ctggcgcaca cggttgtgct gctcaatagc ggtggtgacg
      961 ctacccagac ggccgctatt ttaagtggtt tgaccgctga gcaatccact attgagcgcg
     1021 cgctggccat gaactggctg gcgaaatata tggcgacaat gcctccagtt gttttgcctg
     1081 cgcctgcggg cgcatgggct aaacataagt taactggagg gggcgaagac tggcgttggg
     1141 ttggtcaggg tgtgccggac attctctctt ttggtgacga attatcgccg caaaatgtgc
     1201 aggtccgctg gcgtgaaccg gcaaaaacgg ctcaacaaag taacattccg gtgaccgttg
     1261 aacgccagtt gtatcggctt atccccggtg aagaagagat gagctttact ctgcagccgg
     1321 tgaccagcaa tgagattgac agcgatgcgc tgtatctcga tgaaattacg cttaccagcg
     1381 agcaggatgc agttctgcgc tatggtcagg tggaagtacc gctcccgccg ggagccgacg
     1441 ttgagcgcac aacatggggc atttcagtca ataaacccaa cgccgcgaaa cagcaggggc
     1501 aattgctgga aaaagcgcgt aatgaaatgg gcgaactggc ctatatggtg ccggtgaaag
     1561 aactgacggg aacggtcact ttccgccatt tgctgcgctt ctcgcaaaaa gggcaattcg
     1621 ttctgcctcc tgctcgttat gtgcgttcct atgcacctgc gcagcaaagt gttgcggcag
     1681 ggagtgaatg gaccgggatg caggtgaaat aagtgaactg gcgcagaatc gtctggctat
     1741 tggcgttggt tactctgcca acgcttgcgg aggagacgcc gttacaactg gtgctgcgcg
     1801 gtgcgcagca cgatcaactg tatcagctgt cgtcatcagg cgtgaccaaa gtctcagcat
     1861 tacctgactc gctcaccacg ccgttaggga gtttgtggaa actgtatgtt tacgcctggc
     1921 tggaggatac ccatcaaccg gagcagccct accagtgtcg cggaaactcc ccggaagagg
     1981 tttattgttg tcaggctggg gagagcataa cgcgcgatac cgcgctggta cgttcctgtg
     2041 ggctctattt tgcgccgcag cggttgcata ttggcgcaga tgtgtggggg caatactggc
     2101 aacagcgtca ggcacctgcg tggttagcgt ctcttacaac gctgaagcca gaaacctcgg
     2161 taacggttaa atcgctgctc gattcattag cgacgttacc tgcgcaaaac aaagctcagg
     2221 aagtcttact ggatgtggtg ctggacgagg cgaaaatcgg tgtcgcatcc atgctcggta
     2281 gtcgggtacg ggtgaaaacc tggagctggt ttgccgatga taaacaggaa atccgccagg
     2341 gtggttttgc tggctggtta accgatggta caccgctgtg ggttactggc agtggcacca
     2401 gcaaaacggt actaacccgt tatgcgacgg tgcttaatcg ggttttgcca gtgccgacgc
     2461 aggttgccag tgggcaatgt gtagaggtgg agctgtttgc ccgctatccg ctgaagaaaa
     2521 tcacagcgga aaagagtacg acggcagtaa aacccggcgt acttaatggg cgctatcgtg
     2581 tgacgtttac taatggtaac cacattacgt ttgtcagcca cggtgagacg accttactga
     2641 gcgaaaaggg caaactgaaa ttgcagtcgc atctcgatcg tgaagagtac gttgccagag
     2701 ttctggatcg cgaggcgaaa agtacgccac ctgaggcagc gaaagccatg accgtggcga
     2761 tacgtacttt tttacagcaa aacgccaatc gggaaggtga ttgcctgacc atccccgaca
     2821 gcagcgccac gcagcgtgtt tctgcttcgc cagcgaccac gggggcgcga acgatgaccg
     2881 cttggacgca ggatctcatc tatgcaggcg atccggttca ttatcatggc agtcgcgcta
     2941 ccgaagggac actttcctgg cggcaagcca cggcccaggc ggggcagggg gagcgttacg
     3001 accagatact cgcgtttgct tatcccgaca acagccttag ccgctgggga gctccacgct
     3061 caacctgcca gttattaccc aaagcgaaag cctggctggc gaaaaaaatg ccgcagtggc
     3121 ggcgtatatt acaaggcgag acggggtaca acgaaccaga cgtgtttgcg gtctgtcgtt
     3181 tagtctcagg tttcccctat accgatcggc agcagaaacg gctgtttatt cgtaacttct
     3241 ttacgcttca ggatcggctg gatttaaccc atgagtatct gcacctggcc tttgatggtt
     3301 atcccaccgg gcttgatgag aactatatcg aaacgctgac ccgacaatta ttaatggact
     3361 aatgctatgc gaaagatttt tcttccgttg ttactggtgg cactttcgcc tgtcgctcac
     3421 agtgaaggtg tgcaggaagt cgagattgac gcaccgctct ctggctggca tccggcagag
     3481 ggcgaagatg ccagtttttc acaatcgatc aattacccgg cgtcgtccgt caacatggca
     3541 gatgatcaaa atatctcagc gcagatccgc ggcaaaataa agaattatgc tgcggcgggt
     3601 aaagttcagc agggccggct ggtggtcaac ggtgccagta tgccgcagag aattgaatcc
     3661 gatggttcat ttgcacgccc ttatattttc actgaaggca gcaacagcgt gcaggtcatc
     3721 agcccggatg ggcaaagccg gcaaaaaatg cagttttact caacgtcggg tataggaacg
     3781 attcgtgcac gtttacggct ggttctctcg tgggatacgg acaataccga tctcgatctc
     3841 catgttgtca cgcccgatgg cgaacacgcc tggtacggta acaccgtgct gaaaaacagt
     3901 ggtgcactgg atatggatgt cacgacgggg tacggacccg agattttcgc catgccagca
     3961 ccagttcacg ggcgctatca ggtgtatatc aactactatg gtgggcgcag cgaaacggaa
     4021 ttgacgaccg cccaactgac gctgatcacc gatgaaggat cggtcaatga gaaacaggaa
     4081 acatttattg taccgatgcg taatgctggc gagctgacgc tggtgaaaag ttttgactgg
     4141 tgattaatgg ggttaccttc gccgtcgatg ttcaagaaaa cgcccgataa ctttcgctat
     4201 cgggtgtttt tattgattaa ttcaaccgtt caatcaccat cgcaattccc tggccgccac
     4261 caatgcacag tgttgccagc cccagcgttt tatcgcgtgc ctgcatggca tgtaatagtg
     4321 tgaccagaat acgagcacca ctggcaccga taggatgccc gagcgcgatg gccccgccgt
     4381 tgacattcac tttctcagaa tcaaagccca gggttttccc aacggcgagg aactgtgcag
     4441 caaatgcttc attagcttcg atgagatcaa tgtctgccag ttgcagcccc gccagttgta
     4501 acgctttttg cgtggcaggt actggcccca tacccatcaa tgcgggaggc acgccaccgc
     4561 tggcataact tttaatgcga gccagggggg taaggcctgc tgccagcgcc gcagattctt
     4621 ccataatcac cagagcggca gcaccgtcgt taataccaga cgcgttccca gcggtgactg
     4681 ttcctgcttt atcgaaggcc gggcgcaatg cacctaacgc ttcagccgtt gaattcgctt
     4741 tcgggaattc gtcttgactg aagacgaagg ttttcttccg agtgacaaca tttaccggga
     4801 cgatttcggc tgtaaaagca ccggactcaa ttgcggctgc cgctttacgc tgtgaatgta
     4861 gcgccagttc atcctgcatt tcacgggtaa ttccgtactc tttagccacg ttttcggcgg
     4921 taatccccat atgataacca tgggtggcgc acatcaggcc atcgcgcagg attacgtcat
     4981 aaacctgtcc gtctccaaga cgataaccag agcgtgcttt tgcatcgagt aagtagggcg
     5041 ctaaactcat attttccata ccccccgcca caatgctctt cgcctgacct gcctgaattg
     5101 cctgggcggc aagcgccaca cttttaagac ccgaaccaca cactttattg accgtgaatc
     5161 cgcacaccgt ttctgccagc ccgcttttta acagtgcctg acgtgccgga ttttgcccca
     5221 acccggcttg caacacgtta cccataatca cttcatcaat gtgtagtgaa tcgatttttg
     5281 cacgttcaat ggcggcttta attactgtcg cccccaggtc gatggcgctg gtggaagcga
     5341 gtgaaccgtt aaaactaccg atagcagtac gtaccgcact gacgatgaca caatttttca
     5401 ttttatattc cttctgtctg tagggttgcc gtcagaacag cgttaaacca atgacgaaaa
     5461 tgacgccgga gaagagcagg gcagtgatgc aatagcccat gatgtcgcgg acaccgagtc
     5521 cggcgattgc cagtgctggc agcgcccaga atggttgttc catgttcatc cattgctcgc
     5581 cgtaggcgat cgccattacc gatttaccga gatcagcgcc cagcgcctgg gctgcgggta
     5641 tcacgaaagg tccctgaata acccagtgac cgccgccaga cggaacggcg aagttaatca
     5701 gtgcagaact aaaaaaggtc attaccggga aggtgtcttt gttcgcaaca ttgatgaaga
     5761 attcggtaat gagtccaccc agaccagaat gctccatcat cagttggatc ccagcgtaga
     5821 aggggaattg caccagaata ccggcagtac tgcgtgctgc cgcgctgata gcacgcatat
     5881 aagccattgg cgttttatgt agcaacagac ccgcaatcat aaacatcagg ttgacggtat
     5941 tgatggtgat gttgaagcca tgttcgctga agtacatcgc caggtaagcg ataccgagtg
     6001 cgccgatgat caacgtcaga atgcggcttt cttccagtcg ctcggatggt ggggcatctt
     6061 tcggtagctg cttttgaaaa tcagcctctt ccatgagtag ttccggatcg atactcacca
     6121 cgtcagacgg ttttggcatc atcatgcggg tgataaatgg catcaccaca atcaacgtca
     6181 cagtgatgaa aatgttaaaa ccactgaaca gagtatcgcc caccgggatc agcccggcga
     6241 tatgctcaac cgggttgccc ggtgttgcag ccaacagagg cattgatcca gagaagccgc
     6301 caccccaggt gagaaaacca atgtaggcgc aggcaatgag caacggataa tcagaaccgg
     6361 ggactcgccg ggcgacttca cgggcaaaca ttgcgccgac aaccaaacca aatccccagt
     6421 tgatgacaca agcgactgaa ccgaagaaag tgaccagcat gacgccctgt acgggcgtct
     6481 ttgcggcgga ggcggcagta cgcagcaaac ttttcaccgg agcagagctg gcaagggcat
     6541 gaccggtaac gatgataagc gccatctgca taccaaacgc cagcaagttc cagaaaccgt
     6601 caccccacat tttcaccatg ctgatcggcg tttgtggtgt taaccaaagc gcgatcacga
     6661 atgttagcaa tgtcagcaac atggcaaaga tcagtggatc gggaagccat cggctgacaa
     6721 aacgcgtcat aaaacgcgat atgcgaccaa tcataaatca ccccgttgcg tattcagatc
     6781 ggcggcgact tcaaaccgag cttctgtttt ggcacgcacg gtggctaaat cacacccgtc
     6841 ggcaatttcg gtgagccaca ttttgccgtc aataaaacga aagacagcca gttcagtaac
     6901 cagcatatgc accgcatgtt gcgcagtgag tggcatggtg cagctgcgca aaatttttgc
     6961 tgaaccatct ttggcgcaat gttccatggc gatgatcact ttgcgcgacc cggtcaccag
     7021 atccatcgcg ccacccatac cgggcaccat tttcccaggc actacccagt tcgcgaggtt
     7081 tgcttcttcg tctacttgca aaccgccgag cacgcaggca tcaatatgac cgccacggat
     7141 tagcgcaaat gacatggcgc tatcaaacat ggctgcaccg ggtaaaacac cgcacggttg
     7201 cccgccagcg ttcaccagat ctggatgcgc tgtcgtgacc gggcctaaac cgaggaagcc
     7261 gttttccgat tgcagagtga tatgaatacc ctccggtaaa taattggcga ccattgtggg
     7321 taaaccgatc cctaagttaa cgatgtcacc atcacgaagc tcttgcgcca cacggcgcgc
     7381 aatacgttgt ttcgcatcca ttatttgctc tcctgtgaaa cgatgatgtg gtcgataacg
     7441 gcaccagggg tgacaatatg gtcaggttgc agctcgccgg tttcgaccag ttcatctggc
     7501 tctaccagcg tgatatcagc cgcaagggct atcagggggt taaagttgcg ggcgctaagt
     7561 tgataggtca ggttgccaag tgtgtcgcaa cgatgagcgc gaattagcgc caggtcggcg
     7621 cgcagtgggc gttcgagcag ccaggtttta ccgtcgagtg tcagtgtctg tttgccttcc
     7681 tctacgacgg tgccgacacc cgttggggtg agaaaaccac caagtccagc tccaccacag
     7741 cgaatttgct cgattagcgt accttgcggc accagaacga cgtccatctc accagatatc
     7801 atgcgccgac ctgtttccgg gttggtgccg atatgtgaag caatcacttt gcggactcga
     7861 ccattgacga tgagcggacc gatgccggta tcaacaaacg cggtatcatt ggctatcaat
     7921 gtcaggtcgc gaacaccaga ttccagtaat gcttcaacca ggcgggatgg agtgccaatc
     7981 cccataaatc cgcccaccat gatggtcatg ccgtcacgaa agaagccggt ggcgtcttgt
     8041 aatgtcatca attttgtttt cattttttat ccctcttgca tacaccgttg tgggtacagt
     8101 caggcaatag caaggggagt gccagaatga gattattgga tttagttatt taactaattg
     8161 aatatgaagg gataaattgg atggggattt ataaggagaa tgagcggcta tgcagaaaat
     8221 tgcgcactgt gcaaatttct gcatagcaag ttttggtgtt atacatccgc cggatcgata
     8281 ccgtattcct ggagtttata catcaatgca cggcgactga tgcccagcat taaagcagtg
     8341 cgggttcggt ttccttcttg ttgttccagc acttccataa tgatgcgttt ttcgacgcgt
     8401 ttaatttcct cttttaaatt acgctcaccg acagaggctg tttttacctc gccagcatta
     8461 cagactggct gacgaatctg tggcggaaga tcctcagaaa aaatgatcgg gcctgaattc
     8521 atcacgacgg cgcgttcaat aacgttagaa agctctcgaa tatttcccgg ccatgaccag
     8581 gcggtaagca gtgacattgc catcggatcg atgtcgataa tatcgcgctg attctcacta
     8641 ctgaattttt gcaaaaagtg attagctaac agggaaatat cttcccggcg atcgcgcaga
     8701 ggcggcagta ttaaatgaat aacgttaagg cgataaaaga gatcttcacg gaaggtgcct
     8761 tcttttacca ttgcctgcaa gtcacggttg gtggcagcaa tgatgcggat atcaactttt
     8821 atggtctgat ggccgccaat ccgttcaaat tcccgttcct gtagaatgcg tagtaatttg
     8881 gcttgtagta ccagcggcat ttcgccaatt tcgtcgagga gcagagtacc ttcgttggct
     8941 cgttcaaata atccctgacg caaggtttgt gcaccagtaa atgcaccttt ttcatgacca
     9001 aacagttcac tttcgagcaa cgattccggc agcgccgcgc agttgacttt aatgaacggc
     9061 ccctttgccc gccgcgaatt gtagtgaatc gctctggcaa tcaactcttt cccggtgccg
     9121 ctttcaccgc taatcaagac gctggcctga gaaagggcaa ttttggcggt gtctttgcag
     9181 atgtccatca tcgccgggct gttggtgaga atgtgccccc attgccagct ggcgctcagt
     9241 gcctggtgca gatgacggat ctcttttttc attgactgga gttgtaaagc gcgctgaacg
     9301 attaaattca actcatcgag atcaaacggt ttaataacat agtcgaaggc tccgcagcgt
     9361 agcgcttcga cggcggtttc cacttccgca taggccgtca tcagaataac gggtgtccgg
     9421 gtctcatggc tgcgcatctc ctttagtgcc ttgatgccgt ccatctctgg catgcggata
     9481 tccatcaaca ccacatcagg gtgaatatcg gcaaacaggt gtaatgctgt gcgtccgttg
     9541 ttcgcacaat gtgtttcgaa tccttgtagt gcaaaagcgg tgctcagcat acggcgaaca
     9601 ttatcttcat catccacaat aaggatgcga ttaatagcag tcatacagtc tgatttccct
     9661 gcgggttgat cggtaaaata agcgtgaagg ttgcgccgta gcccggcaaa ctggcgacgc
     9721 gaatatcacc ctgatgggca ttaatgatgc gttgacttaa cgccagacca agcccggttc
     9781 ctgaggcttt ggtggtgaaa aagggatcga agatcttttt ttgcagcgag agatcaatgc
     9841 cacagccgtt gtcctctatc gaaatggcct gttgtgagtc gctgtattgc caggtttgaa
     9901 tgcgaatttt ccctcgtgcg ctgatagcct ggacggcatt gatcaggata ttcagtagta
     9961 cctgtttgag cagttcacga tcggcgttaa tcgggcttaa ttcattatcc agttcgctta
    10021 tgaagtcgac ccgcgcttgt acgccggcgg tttgtaccag taccagagtt tcttcaacca
    10081 atgcattgag gctgacttgt tgccattgac tgtggcgtgg acgtgaaaat tcgagcaatt
    10141 gctgaataac tttgttaatt gaatcgattt ctttgagtac tacggacaga tattcctgat
    10201 gtattgggtc actggtttgt tggcgcaaga tctgtacata accacgaata gccgttaacg
    10261 gattacgtac ttcatgcgcg acgccagcca tcagctcacc cagtgtggcg aggcgttctg
    10321 cttgcgccat gcggcgctgg gtttctttgc gggcagttaa atcagagaaa atcaccaaag
    10381 cacctatcat ttcaccgtgc gtgttatgaa tacgactggt agtgacactg agttcaatgg
    10441 tgcggtcacg acctggaaaa ctgatctcca gcgccacatg ttcggtgcca tgttccagcg
    10501 tatccagtac tggactgtag aactgagtat tgtcgaacaa catggagtaa ggctgcccta
    10561 ccagttcatg gcgttgatag ccagtgataa cttctgctgc tgggttcatg gtggttacat
    10621 caccctggcg gtcaatggca atgacgccat cggcagcgtt ttcaataatc agatcgttaa
    10681 gtgtccgcgt ttcacgcagt gcctgggcga ggttattaac actctgactg atttgcccca
    10741 tttcaccggg caattgtggt aatcgagtgg gaatattttg tgccagagtc gagaggccat
    10801 cggtgatgat atcaatattg gcgctcaggc gacgggagaa aaggacaatc aatagcaggc
    10861 ttatcagcaa atcggcggtg agcacaatga taatcctcac atccattttc caggcctggc
    10921 ggcgaatatc ttcggttaat tcattggccc agatatagcc gaggatttca ccattacgct
    10981 caatggggag cattgaattc aaaatatcgc cgcgcacctg cctgcctgaa taaaccaaag
    11041 gggtatttgt acgcatgact tcgcgaccag ggtgatctgc ggcaatggta acgccgacat
    11101 tattctgata tagcgctgaa ggcgcgtagg tgattatcgc atccagcatt ttgttgtaat
    11161 aaccagcacc tatgccaggg aaggcgtgag tgatattttc ggtaatgggg gcaagttctg
    11221 catttaatgc gcggatacgc tcctcacgtg gtaagtcgat gtagagatca tagcgatcgc
    11281 ctagtgcctg attaagcagg ttgaccacgg cagaaagttt tttctctttt tcagataaga
    11341 ctgctgaacg tccttccgtt tctacgatat aaccaatagt aagcgttggg acaatgacca
    11401 tcaggattgc catcaggatc atttgattgc gtaagcggcg tggataaatc cacttcatat
    11461 aatgcatgct gaaatccact agtcttgtcc ggtatatgac gattatcaga ggttaaggtg
    11521 atgatttctc ggcggtgtat catattccag agaagagaga acattgcggt aacacgcttt
    11581 taccgctacc ttaaccacac tccatcggtc acctgaggcg gagcttcgcc cctttgaaat
    11641 accttgcttc ttttcgtaca accctgaaag cctcgcgcta catgttcaga gcattggcgt
    11701 tagtgctctg gctgttgatt gctttttcat ccgtttttta catcgttaat gcgttacatc
    11761 agcgagaatc ggaaattcgt caggaattta atctgagttc cgatcaggct cagcgcttta
    11821 ttcaacgcac ctctgatgtg atgaaagagc tgaagtacat cgccgaaaat cgcttatcgg
    11881 cagaaaacgg tgtgctttcc ccgcgtggac gagaaacgca ggcggatgtg cctgcgtttg
    11941 aaccgctgtt tgccgactcc gattgttccg caatgagtaa cacctggcga ggttctctgg
    12001 agtcattggc gtggtttatg cgctactggc gcgataattt ttctgcggct tacgatctaa
    12061 accgggtatt tttaatcggc agcgataacc tctgcatggc caatttcggt ctgcgtgata
    12121 tgccagtgga acgcgatacc gcgttgaaag ctttgcatga acgcatcaat aaatatcgaa
    12181 atgcaccaca agatgatagc ggcagtaacc tctactggat cagcgaaggt ccgcgccctg
    12241 gcgtcgggta tttttacgcg ttgacgccag tttatctggc gaaccggttg caggcgcttt
    12301 tgggtgtcga gcagaccatc cggatggaga actttttctt accgggtacg ttgccgatgg
    12361 gggttaccat tcttgatgaa aatggtcata ccctgatttc gcttaccgga ccagaaagta
    12421 aaattaaggg cgatcctcgc tggatgcagg aacgctcctg gtttggctat acggaagggt
    12481 tccgggagct ggtgctgaag aaaaatctgc caccctcatc gctaagcatc gtgtattcgg
    12541 tgccggttga taaggtgctg gaacgcattc gcatgttgat ccttaacgca attttgctga
    12601 atgtgcttgc cggagctgca ttgtttactc tcgcacggat gtacgagcga cgtattttca
    12661 ttccggcgga aagcgacgcc ctgcgactgg aagaacatga gcagttcaat cgcaagattg
    12721 tcgcctccgc gccagtgggt atctgcattt tgcgtaccgc tgatggcgtc aatattttaa
    12781 gtaacgaact ggcgcatacc tatctcaata tgcttacgca tgaggaccgc caacgactga
    12841 cgcaaattat ctgtgggcag caggtcaatt ttgttgatgt cctgaccagc aacaatacca
    12901 atctgcaaat cagcttcgtc cattcgcgct atcgtaatga aaacgtggcc atttgtgtgc
    12961 tggtggatgt ttcttcgcgc gtgaagatgg aagagtcgtt gcaggagatg gcacaagcag
    13021 cggaacaggc gagccagtca aaatcgatgt tccttgccac cgtcagtcat gagctgcgaa
    13081 cgccgctgta tggcattatc ggtaacctgg atctgttgca aaccaaagag ttaccgaaag
    13141 gcgtcgatcg gctggtgacg gcaatgaaca actcttccag cctgttgttg aaaattatca
    13201 gcgatattct cgatttctcg aagattgaat cggaacagtt gaagatcgaa ccgcgtgagt
    13261 tttcaccgcg tgaagtgatg aaccacatca ccgccaacta tttaccgctg gtggtacgca
    13321 agcagttagg cttgtactgc tttattgaac cggatgtgcc agtggcctta aatggcgacc
    13381 cgatgcgttt acagcaggtc atctccaacc tgttgagtaa cgccataaaa ttcaccgata
    13441 ccggctgtat agttttgcat gtccgcgctg atggcgatta tctctctatc cgtgttcgcg
    13501 ataccggcgt ggggattccg gcgaaagaag tggtgcgctt gtttgatccc ttcttccagg
    13561 tcggaacggg cgtacagcga aacttccagg ggaccggtct gggtctggcg atttgtgaaa
    13621 aactgatcag catgatggac ggcgatatct cggtagattc agaaccggga atgggcagcc
    13681 agtttaccgt gcgtattccg ttgtacggcg ctcagtaccc gcagaaaaaa ggcgtggaag
    13741 ggttgagtgg taaacgctgc tggctggcgg tccgcaatgc gtcgctctgt cagttcctgg
    13801 aaaccagttt gcagcgcagc ggcatcgtcg ttacaacata cgaagggcag gaaccgactc
    13861 ccgaagatgt gttaattact gacgaggtag tgagtaaaaa atggcagggt agagcggtag
    13921 tgaccttctg tcgtcgccat attggtattc cgctggagaa agcgccaggg gagtgggtac
    13981 acagtgtggc tgccccgcat gagctaccgg cattgttggc gcgtatttat ttgatcgaga
    14041 tggagagcga cgatcctgct aacgctctgc cgtcgacgga caaagcggtc agcgataatg
    14101 acgatatgat gattctggtc gtggatgatc atccgattaa ccgacgtctg ctggcagatc
    14161 agttgggatc gttgggctat caatgtaaaa ccgcgaatga tggtgtcgat gcgcttaatg
    14221 tacttagcaa gaatcatatt gatatcgtgc ttagcgacgt caacatgcca aatatggatg
    14281 gttaccgctt gacgcaacgc attcgtcagt tgggactgac gttgccggta atcggagtaa
    14341 ctgctaatgc gttggctgaa gagaagcagc ggtgtctgga gtccggtatg gacagctgcc
    14401 tgtcgaagcc ggtaacgctg gatgtgataa aacagacgct gacggtatat gccgagaggg
    14461 tcaggaaatc gcgggaatcg taggacggat aaggtgttta tgccgcatct ggcattcagt
    14521 gcaaatgcca gatgcgacgc tgacgcgtct tatctggcct actttgatgt aatctgttga
    14581 aataatggga atcgtaggac gcataaggcg tttacgccgc attcggcaat cggtgcaaat
    14641 gccagataag acactaacgc gtcttatctg gcctacaggt gattagtctt tatctgccgg
    14701 acttaacgtc actgaagaga gataattcag cagggcgata tcgttctcga cacccagctt
    14761 catcatcgca gatttcttct ggctactgat ggttttaata ctgcggttca gctttttagc
    14821 gatctcggtc accaggaagc cttccgcaaa caggcgcaga acttcactct cttttggcga
    14881 gagacgcttg tcaccgtaac caccagcact gattttttcc aacaggcgag aaacgctttc
    14941 cggggtaaat ttcttccctt tctgcagcgc ggcgagagct ttcggcagat cggtcggtgc
    15001 accttgtttc agcacgatcc cttcgatatc cagatccaat accgcactaa gaatcgccgg
    15061 gttgttgttc atagtcagaa caatgatcga caggcttggg aaatggcgct tgatgtactt
    15121 gattaaggta atgccatcgc cgtacttatc gccaggcatg gagagatcgg taatcaacac
    15181 atgcgcatcc agtttcggca ggttgttgat cagtgctgta gagtcttcaa attcgccgac
    15241 aacattcacc cactcaattt gctcaagtga tttgcgaata ccgaacaaga ctatcggatg
    15301 gtcatcggca ataattacgt tcatattgtt catgtaatag gctaccttgc tacagcaagc
    15361 tcttgacata actgtcaatg tcgctgatgt atttttctat tcctggaaca tccttctcac
    15421 gaatcagatg ttccagcgtt tcacataact gcttgccggg taccagattt agcatggcaa
    15481 atacgccttt aagacgatga gccgtttggg ctaacgcagc aaagtcactg gttgctgctt
    15541 cagtatacag cctcttaaca tcatccggta ctgtgtctac aaagagcgca taatagccgc
    15601 tggcatggag ttgcgcattt tcatctccgc ccagaggcga ttctgtcacc tcttcctgcg
    15661 ccagttgcac ttcaattaat tgtaagaccg cttcctgcat agcgttgctc atattgaagt
    15721 tgacgcacaa ttgaccaggc ccaatttccc gtacgccaga ctcatcatcg cttaaaagca
    15781 agccagaggc agtaagatta gacggattat ccgttaaaaa gatatcataa tcttgactaa
    15841 ttaatctttc atcgggtgtg atacaggttg caccccaatt ttctaactgg cgagtgacaa
    15901 tattccgaat ttctgccgaa gtaacatcca ccattacgca gacatcatcc agtaaacgct
    15961 cttcttcctc ttcaacttcc gggccagcgg cgagcatttt gatatgcaca gagtagcgtg
    16021 taccaagccc atcccgcgtt ttgatgttta aatgaccgcc cagtttacgt gccagttgat
    16081 cgctcagcca gaatgccagc gggtccgcct ttccataacg atcgttttgg gtctggttga
    16141 taaacgggaa gtgcaaatta tccatttcat gaatacttac gccttctccg gtgtccagaa
    16201 tgcggaatgt caggcggtct tcagaggact catcctgatc aacctcaagg gtgatttttc
    16261 ccaattgcgt tgaggtcacg gcatattgca tcagtagcag caaaatacgt cgtaaggcat
    16321 cgcgatcgcc gcggcgcata tcgtgtgctt tcagatgatt gttaatcagc agttgcagac
    16381 ctttacgctt gatggcaggc aacactgaag gcacaacttc atcaattaaa tcctgcacgg
    16441 agaacagcac cgtctcactt ttccagctat cgtccgcaag catgttcgct aactgtattt
    16501 catcgaccag ccgcaccagc acatctgcct gattcgccag ttgtttgctt tccggggcgt
    16561 tgagtttagc cgcgctctcc gccagggact gtgcaggttc tttcagcgca tcgccaatgt
    16621 ttttcataaa ggtcatccgg ccctgctggt ttttctcata cagacgctgc gcctgcttga
    16681 gtttcttgtt taccagcact tcgcgatcct gatcgcgaat aatgaaaatt tgtgtgcgcg
    16741 gcgcgacctg gctgcggaac atgcggatct catacagctc gttattgatc gtcgcctgaa
    16801 taatcccctg atgctgttcc gccatggtgg tgatgttttg cagattcaaa tgcggcagca
    16861 aatgatcggc aattttgtta cttatgacag tgcggttcga ttcctgatcg tgaaccagca
    16921 ggccgagcgg cagcagtgag actatctttt cattgattgc ccgtaaaatg cgcaattcgt
    16981 tattgaccgc cgtgttgggt agactttctg tactgcgact ggagaaatgg cggaatgtgg
    17041 tatagccaaa taacgccagc gccagcaaac cgatgttcag cagcagtggc agcagaatgt
    17101 tttgcaacgt atccagcaat aaggtgccat aaggaacctg ccagaccagg cgcatatcgg
    17161 tagagttgag tgccgaggag atttcaatct tcgtactgtt aaagtggata ctgacgctat
    17221 ccgttccttc tttctcatta tcattgtctc ccgttgccgt cgtgtctggc tcaaggcgga
    17281 aactgtccag cggcataccc ggtgggatca aatcattaat cggcagatca aaagccacga
    17341 ccgttgccag atgtcccggc tggttgaacg tggtacgcaa ggtaaagtaa tgaccgttct
    17401 gccaggccag gcggcgcatg ttagaaaaac tttcgcgttc atcgagggcg ttggcctgtt
    17461 gcaacatttc tgcacgacgt gaatcaacaa tgtcactgac ggtcgattct ttaaatccgg
    17521 aggtgagatc tttgaggggc agggttgaga tcagtaccag actgttatcc tgaccattca
    17581 ggtaatacat cgaccacggt acattttctg caccccacaa tgtatccaga taagtggaca
    17641 tccgctgagt catctcaagc gttgagttgt cgtgagagcc aaagattaac gcttccgttt
    17701 tgcggcgtgg tttctccaga tagtagacat cctgtttcag gcgcgtctct tgtaaacctt
    17761 cgccggagga gggggaggtc gtcgcggcaa tgttgtcgta gatctgccag gtcacgtaac
    17821 gccaggtatc gacgcgcttt tggattgcgt gggtaatgtc gacaatctgg taacttttat
    17881 ctttcagcca ggcgttaacg gcgctttgta ccattacccc catcgtcacc agtaacacaa
    17941 tgatcaacag taaaaagaag cgggtaatgc tccccggtag gagtgaaaag cgggtcgtgg
    18001 ccgttgtctc tttctgacgc attcgtgttt atgacctgtt aaaacttcgc gaagtgatag
    18061 ggcagtataa agggtacagt gtgaaattcc agactcttac ctgattgatg attctctatt
    18121 ctcccgtgat atttatcatc ctgcattggg ggaaatagat gtacaagcgc cattttgctg
    18181 tacaaataac ctacaaaaag cccaacgaaa ttaattgcaa ataaacgaag attgctggaa
    18241 attatgctga tgttaattat ttgtgaaata gttgacaaga gttatagttt ttctgtggta
    18301 gcacagaata atgaaagtgt gtaaagaagg gtaaaaaaaa ccggatgcga ggcatccggt
    18361 tgaaataggg gtaaacagac attcagaaat gaatgacggt aataaataaa gttaatgatg
    18421 atagcgggag atattctagt tgcgagtgaa ggttttgttt tgacattcag tgctgtcaaa
    18481 tacttaagaa taagttattg attttaacct tgaattatta ttgct
//
LOCUS       DAJTYZ010000082        18160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000082 DAJTYZ010000000
VERSION     DAJTYZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.082"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..844)
                     /gene="adrA"
                     /locus_tag="OO596_002677"
                     /gene_synonym="dgcC"
     CDS             complement(<1..844)
                     /gene="adrA"
                     /locus_tag="OO596_002677"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6718499.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(946..1266)
                     /gene="psiF"
                     /locus_tag="OO596_002678"
     CDS             complement(946..1266)
                     /gene="psiF"
                     /locus_tag="OO596_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6718500.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQAAQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1385..2800)
                     /gene="phoA"
                     /locus_tag="OO596_002679"
     CDS             complement(1385..2800)
                     /gene="phoA"
                     /locus_tag="OO596_002679"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6718501.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2901..3161)
                     /gene="iraP"
                     /locus_tag="OO596_002680"
     CDS             complement(2901..3161)
                     /gene="iraP"
                     /locus_tag="OO596_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6718502.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3343..3546)
                     /locus_tag="OO596_002681"
     CDS             complement(3343..3546)
                     /locus_tag="OO596_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718503.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3624..4718
                     /gene="ddlA"
                     /locus_tag="OO596_002682"
     CDS             3624..4718
                     /gene="ddlA"
                     /locus_tag="OO596_002682"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6718504.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAKVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4742..4954)
                     /locus_tag="OO596_002683"
     CDS             complement(4742..4954)
                     /locus_tag="OO596_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6718505.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5214..5522
                     /locus_tag="OO596_002684"
     CDS             5214..5522
                     /locus_tag="OO596_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6718506.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5581..6675)
                     /gene="yaiW"
                     /locus_tag="OO596_002685"
     CDS             complement(5581..6675)
                     /gene="yaiW"
                     /locus_tag="OO596_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6718507.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6688..7908)
                     /gene="sbmA"
                     /locus_tag="OO596_002686"
     CDS             complement(6688..7908)
                     /gene="sbmA"
                     /locus_tag="OO596_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6718508.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8260..9417
                     /gene="ampH"
                     /locus_tag="OO596_002687"
     CDS             8260..9417
                     /gene="ampH"
                     /locus_tag="OO596_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6718509.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9418..10041)
                     /gene="iprA"
                     /locus_tag="OO596_002688"
     CDS             complement(9418..10041)
                     /gene="iprA"
                     /locus_tag="OO596_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR6718510.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10129..13017)
                     /locus_tag="OO596_002689"
     CDS             complement(10129..13017)
                     /locus_tag="OO596_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6718511.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            13543..14517
                     /gene="hemB"
                     /locus_tag="OO596_002690"
     CDS             13543..14517
                     /gene="hemB"
                     /locus_tag="OO596_002690"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6718512.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14623..15474)
                     /gene="tauD"
                     /locus_tag="OO596_002691"
     CDS             complement(14623..15474)
                     /gene="tauD"
                     /locus_tag="OO596_002691"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6718513.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(15471..16298)
                     /gene="tauC"
                     /locus_tag="OO596_002692"
     CDS             complement(15471..16298)
                     /gene="tauC"
                     /locus_tag="OO596_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6718514.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(16295..17062)
                     /gene="tauB"
                     /locus_tag="OO596_002693"
     CDS             complement(16295..17062)
                     /gene="tauB"
                     /locus_tag="OO596_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6718515.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(17075..18037)
                     /gene="tauA"
                     /locus_tag="OO596_002694"
     CDS             complement(17075..18037)
                     /gene="tauA"
                     /locus_tag="OO596_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6718516.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         4527 a         4722 c         4390 g         4521 t
ORIGIN      
        1 taccgcgcag ggtaatttgt aactgtcggg taagcgccac aatcgcttca tcgcccacat
       61 catgacccca ggtatcgttg atgctcttga aatggtcgat atcgataatc agtaacgttg
      121 catcgcgatt atgccgccga cagttatcaa attcattgcg taacatagtt tcccaatgac
      181 gtcggttata cacgcccgtc atgccgtcgc gagtactcat gacctgcaac ctgcgtttat
      241 gttccgccag tttggttgcc gtctggtagc tgacccagcc aaacagcaga ggataaacga
      301 caataatggg aagggagagc caccattcca gcggcgcact attgaacgac acggtaatgc
      361 ccgtaagctc gagggtgaca aggcaggaaa ccaccatcaa caccagaccc gcgacaaaca
      421 gacgggggcc gcctgccccc atcaaattca gacacataat catcaacata gcggtggaag
      481 gcagcacgtt tacgcccatt acgcctaccc acattcccgc taatactgca tcggttttta
      541 agttgtaaat ttcccggcta agcggatcga cggccctgct cgctatctgc caggctaaat
      601 gcggccagac gaacgcccag ccgaccaaca ccagccacca ccagcccggc ggcgggtgcg
      661 aaaccagcgt tgaagcaatc ggtaagaaca tgccagccag gccaaccgca cggggtagtc
      721 tgacgcgacg ggcgaagcgc agcccggacc gctgatgttc gttttgagga gttaaaggag
      781 gttcctcccc atgcgccgcc gcttttttga aaaagttttc atcattcatt atttttggga
      841 acattcagaa acaattttcc caaattatag agacggggct aaaggcagaa gtatgaaatt
      901 tcgggtgagc cactttcgta gctcacccgt tagcactatc aggtatcagg cggctttctt
      961 gaggcaggca ctcataaact tattacgatc atcacctttc agagattgtt gcgctgcttg
     1021 attattacat tcgcgcatct tttgctgctg tggcgtcaaa cttttttcac caggcgcaga
     1081 cttgctgttc ttcaggcaat cactcatgta ggtcttacga gcatcccctt tcaacgcctg
     1141 cgccgtcgcc tgctgattac aggaggtcat acgctgttgt tgtggggtta aggttctctc
     1201 ggcagcgccg acggtggtta aaaaaaccag accaaaaagc aaggtaacca gtaatgttat
     1261 tttcatagca ccatccctct tcatgtttta accatgagcg tttgcgcccg tgatctgcca
     1321 ttaagtctgg ttgctaacag caaaaaaacc acccggcagc gaaaattcac tgccgggcgc
     1381 ggttttattt cagccccaga gcggctttca tggtgtagaa gagatcggtc tggtcggtca
     1441 gtccaacaac attggcggca tgcggaccat acgccgcaat acgcaactga ctgccggtat
     1501 gttcttgtga atcctcttcg gaattcccgt aactcatcac catcactgcg ccatctttag
     1561 tatttagcgc ctgagtgagg cccggcgctt tggtatccgg cgcaacaacc tggctggcgt
     1621 gggcgtgatc agcggtgact atgaccagcg tgttaccctc ctttttagcg aattccagcg
     1681 cccgttgtac ggcttcatcg agatcgaccg tctcgccaat ttgcccacaa ggattcgcag
     1741 cgtgatcctg tttatcgatt gacgcacctt caacttgcag gaaaaatcct ttctcatttt
     1801 tactcaacaa ttcaatggtt ttgtcggtca tctgtgccag ggttggtaca ctgtcattac
     1861 gttgcggatt aggcgtacag gtgactgcgg gcttatcgat attgccgtgg tacgttgctt
     1921 tcggccctag ccagcgcact ggcatattgc cgtcagcaaa caggccaagc aggggttttt
     1981 gctgattcgc ttccgtcacc gaattcagtg aggcagcatc gttcaccaac tgataaccac
     2041 gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc accagcggtt gccgtttcag
     2101 caaaggtttt tgcgccgccg ccaagcgtaa cgtcggcacg agcgttaagc agctgttcgg
     2161 taatcgatcc ttttccgcct ttttccagag cgttacccgg acatttttca ctggtcgcgc
     2221 tcggaccgta gcatttgcgc gaagtcacat gtgccaccag cgcagcgggc gtggcatcct
     2281 gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc ggcttttgcc atttccagaa
     2341 tcgttgggtg atctttttcg tgaatatcga cgcccagcgc gccgttatag gttttgacac
     2401 cggttgacca ggcggttgct gatgcagccg agtcggtgac gtagtccggt ttgccagttt
     2461 ttttattcag cgcatagtga gtgtattgcc cggtaagcgg taaggcatcg atacctttaa
     2521 aaaagccgcc cgcaccttcg gcataattac gtgcggcagt aatttccgaa tcccccatcc
     2581 catcgccaat cagcaaaata atattttttg caggtttatc gctaagagaa tcacgcagag
     2641 cggcggtctg atcactcgtt aagcggcgag caccgccggg tgcagtaata tcgccctgag
     2701 cagcccggtt ttccagaaca ggcatttctg gtgtccgggc ttttgtcaca ggggtaaaca
     2761 gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac tttattttct ccatgtacaa
     2821 atacattaaa aaataaaaac aaagcgacta taagtctcgg ccgtgacaac tttatgacag
     2881 ctgttgaaaa gattaacttt ttactgacga ggatgcttca ataacttctt tacgtaatcg
     2941 cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg gctttacctc gtacagcgcc
     3001 ccctctacct gatcaatcaa ccgctgttgg tcattttgcg ccatattgcg aagcattgca
     3061 gtgacgataa tctccaaagc ttctacctgc gcacacagtt ctttcgactc ttcttctttt
     3121 tgggcaagct taaataacaa ctcagcaatg agatttttca tgtctgtatt tccttatcca
     3181 aagtatggag aagttaacat tatgtttcac aacttcatag tgtctggaag tattattttt
     3241 atgacaaagg aaatactttg cgcaaatatt ttttgtttga ttaccagcga aacgtttctc
     3301 ttatttaaat aattacccac acaaaatata ggcttttaat tatcaacctt tattataaat
     3361 aaaagttatg aaatatattc tcttcgttaa cttataatta agagaaaaaa actcaccgca
     3421 ttgccattta agccaggaat aaattagctt cccgttcatc atttttgtga tctcgcgcac
     3481 atctttgaga taacgcactt tcttcaccag tacgttgcac gctttgtcag cagaagattg
     3541 caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta tgcactttgt
     3601 gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt tttggtggta
     3661 aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat gccattgata
     3721 aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg cacgtcagcg
     3781 atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg cgcccttcgg
     3841 cgaccagcct tgcgaaggtg ccaggtaaac atgagcatca gcttatcgac gcgcaaaacg
     3901 gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg ctgggcgaag
     3961 atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt tctgatgttc
     4021 tggcttcagc agcctgtatg gataaagatg tcaccaaacg tctgctacgc gatgccgggc
     4081 tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc agttttgccg
     4141 aagtggagtc taaactgggg ttaccactgt ttgtaaaacc ggctaatcag ggctcttctg
     4201 ttggtgtcag caaagtaacc agtgaagaac agtacgcaat tgccgtcgat ctggcgttcg
     4261 agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc gaatgcgcag
     4321 ttctgggcaa cgacaatccg caagccagca cctgtggcga gatcgtactc accagcgatt
     4381 tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta gttccggcag
     4441 ccattgcacc agagatcaac gataagatcc gggcgattgc cgttcaggct tatcagacgt
     4501 tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac gaagtggtga
     4561 tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg aagctgtggc
     4621 aagccagcgg tctgggttac accgatctga tcacacgttt gattgagctg gcgctgaagc
     4681 gtcacgctgc ggataacgca ttgaaagcca caatgtaata ttttaacgcc cgataatatt
     4741 cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaacccc gccagccaga
     4801 aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt gcttcaaatc
     4861 ccagtaaccc caccacgcca ttaagaatga atataaggcc aatggcgaac gcatagtagt
     4921 gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg aaaaacaaca
     4981 tccttgcata ttctgtaaca accgtctgtg gtgtagacaa cattttcgta attgttaata
     5041 aatttaactt aaggttgcca ttttgtaact cacaacgaag attcacgctt cgggaatatt
     5101 cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt cagacttgcc
     5161 ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt tttatggctg
     5221 atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg
     5281 gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg caattgctga
     5341 acctgttagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag gaaacaggtc
     5401 gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc ccgtttttag
     5461 taggctgcct catttttgca gtggtggcgc tatggctgca ctggcgacat cgccgccagt
     5521 aagatttacg ttgccagata cggcctaaaa cgccttatct ggcatgacat tcctgtttga
     5581 tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt
     5641 caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt cgcggggtag
     5701 tgatttaccc gttttcgctt cggcaagttg ataaactttc ttgtacagcg ccgtctcctc
     5761 aaaagagaag ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc tcatccccag
     5821 ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggttctttac tgtcgtaacg
     5881 aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgactg cgttctgaaa
     5941 cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaagcggt atatcggtgc
     6001 gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc cgccgcggcg
     6061 actgaagact tcctgacgaa ctgtaccgtc cattttccac ggataccctt tggtatgctg
     6121 ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcaccggat taagcgaacc
     6181 aaacagcgtc tgccccattg gtaccatgct gatcagatcg tcaaaaatcg cgctcaattg
     6241 cttttctgta cgcaccgaat ctaaccgttc gctataactt ttgccgtttg gcgatttgat
     6301 ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat
     6361 ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat aatttgactc
     6421 ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca gcccctgact
     6481 ttcaaaggtg atggcgagat ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat
     6541 cttctgccgc acgacgctcg ctacatctat cgctttttcg ccctttttca acggttgcgg
     6601 agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg aagatagatg
     6661 attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt cctgaatttt
     6721 gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca tcaactcaac
     6781 cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt
     6841 ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg gaaacagcaa
     6901 gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt tgaaatacat
     6961 atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg ctaaacagct cgcgtaccgt
     7021 aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac ggtaggcggc
     7081 ctctactcgc tggtttttaa actccagccc cggcagtttg atccctacca ctgccagcca
     7141 tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg gaatgtgccc
     7201 gataatcggc agctccggca catgcgcgga gagcgttacc agcaccggca ggaaggcgat
     7261 caacgtcatg atggcgttga taaaactgac gcccatattc tccagcgttg aagcaaaacg
     7321 catggtgtct tcctgcacac gctgtgcggc accttcgata tgacgcagtt gttgccagtt
     7381 cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acatagtgac tgacaaagaa
     7441 gttgttcagt acactgatca ccaccgcgat cagcgcaatc cccagaaaga cgcccacttc
     7501 gcggtaaaat tgttcgatag tgactttatg cggcgaactt agcgcggttt gaatcagatc
     7561 atagaacggt gcataccagg cgttgacggc gaccccgact tccaccaaaa accaggtgac
     7621 gaagatgatc agtgcagtac cgagtattga ccagtattgc caacggtgcg ggctgtagat
     7681 aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa
     7741 atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc cggtgatacg
     7801 cgccacccag tctcccccac cggcttgcca gaagataacg gcaatcaatg cccaaacaaa
     7861 ggccgagaga aaaaacgctc ccggctttgg gaaaaaagac ttaaacatat gcactcctgc
     7921 taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccacta caaagcgtca
     7981 gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca gcctgaccat
     8041 aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg attaacacgc
     8101 gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac gcgccgttac
     8161 gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga gtagtataaa
     8221 tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag tctgcttttt
     8281 tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc gatcactgaa
     8341 ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta cggcagcggt
     8401 gcaacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg tagttatggc
     8461 gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg tattgcttcc
     8521 ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc tcgaccaggg gaccgtaaag
     8581 ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtgccgac ctacaatggg
     8641 acgcccatta ccctagtgaa tctggcaacc cataccagcg ccctgccccg tgaacagccc
     8701 ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc gcgagcaacg ctggaaatac
     8761 ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg cggcgtactc taaccttgcg
     8821 ttcgacctgc tggcagatgc actggcgaat gcgtccggca agccttatac ccagttgttt
     8881 gaagagcaga ttacccgtcc attggggatg aaagacacca cctacacccc ctcaccggat
     8941 cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa cacgctggcg
     9001 gcaattggca gcggcggcgt ctattccacg cctggcgata tgatgcgctg gatgcagcag
     9061 tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca gacgttgatt
     9121 tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg tccccggcaa agcggatgcg
     9181 cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat tattcagaag
     9241 acaggcggtg gtggcggttt cattacctat atggcgatga tcccgcagaa gaatattggc
     9301 gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag cgatggcatc
     9361 aatgatctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc atcctgatta
     9421 atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt cgatatagcc
     9481 gcctttacgc aaagcagcga gaacttctgc gacgacagaa cgcgatatgc gtgtacgttg
     9541 atggatataa ttcatcacac caatacgtga tcgcaattct tcattccagt caatcattga
     9601 taataatgtt gcgcggattt gttcgtagga attatgccca atgagctgca cgtcgcgtaa
     9661 ttccagaatt ctattttgcc aagctaacca gtaaaaagcg tctcgccaga gttgattttg
     9721 ttcaataagc gtaatggttt gtttggctgg tagatgatat cccgtacaat ttccttctga
     9781 tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca taatataagg
     9841 ggcctgggta ataccgataa gtacgttttc ttctctacgc agagagataa ctccctctaa
     9901 aataacgaaa gtatcttcgt tattgacatc actggaaaat ataacttgct tttcattatt
     9961 aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa attcctgaag
    10021 aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca aatccttgac
    10081 gggcattatt tagattaaat taccagtatt tcttcggagt gaagaatatt accaggtata
    10141 tttaacaccc acgttcgcgg accagtcttg atctacgtca ccaccaccga ggtagttagc
    10201 atcggtatag gcgctgaagt tcttggtgaa gctgaactga gtacccagac caacacgtac
    10261 cgcagaccct tcagtaccgt tatcgatgga atcaccgttc acatcgttat cgttgttaga
    10321 gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga gcctgatctt cgctgtaggt
    10381 gaaggtataa cctgcatcta cacccagttc ataacgcatg ctgtcgtaag actgaccgtc
    10441 aactttcatg tcgttgctca gctggtagtc atcaccagac tggaacagac cagaaacgct
    10501 gccgtaagga gtcacgtaac cagcatcacc cagtttgaag tcgtaaccgg ctttcaagcc
    10561 gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa gtaccgttgc tcatggttgc
    10621 agacaggtcg ttgttgaagt gagagtagct caagctacca tcaacaaaga cgttgttcgc
    10681 gaagtgagca gaagagtaga tgtaggcagt ctggctgtct tgatctacct gaccagaacg
    10741 gtcattcatg tcacctttag cgaagcctgc agccgcaccg acgatccact tagcgttgtt
    10801 accgtcaact ttggtatcaa caccgaccat gatgccgtta acatcctgat catagttgat
    10861 ggtgccgttg tcgccgttga agttaccacc gaagtagctt acccatgcgc cgccgttatc
    10921 agccaggcca tgacgagagt tggtcagacg agtaccaacg gtgtcttgtt ccaggttcca
    10981 gatattggtg ttcgcagacg ggatgctcag cgccatgtta gcgtagtcgg tcagctccat
    11041 ctgttgcaga acaacggtgt taccgcgctg ttcagcctga taggtgtatg cacccaggtc
    11101 agctttgtta gcagcagaga aggtcgcgtt ggttttggta tcatcgatat agatgagctg
    11161 cttacctgcg taatcagcaa tcgcaccagc gccagtggag ttgtcgatac gaactttgta
    11221 gttaccggtt gcagctacaa cgttgtctgc agagctgttg tcaacttcgt cgccattgtt
    11281 aatgtcgttg ttaccattaa cggtcaagtg accatcagag ttcatcgcaa taacaccata
    11341 accgtagtta gctttgctgg tgtcgttggt acgatcgttg acgaggtctg cattcaggat
    11401 gtaatcatta ctatgaatgt caaacacacc tgcgtaaaca ttaccgttgt tatctttaac
    11461 gttgccactg gtcagttcca gagtatcagt tgcaaatacg ctggagatgt cattcatgtt
    11521 ggtgtttaca tccagcacac caccgttagt aacggtaatg gtattggcat acagcggtgc
    11581 atatgcccaa gctgaagcag tatcttcacc gaagttaacg gttgagaagg tatcaatggt
    11641 caggtgatcg gtgttaactt caccgttaac ttccattgaa gaaccaccgt tcagagtgat
    11701 ggtgtcagaa gtcagagaag aatctgcact cacgttaacc tgagaaccgc tgttgatcgc
    11761 cagggtatcg atcagggaag ttttcgtggt ttcccaatca gaaccgttgt tcagaacaac
    11821 atcgaatacg ccgctctgga atgcgttagc gttatcaaca cgattccagc catcttcaga
    11881 gtaagaagcg tacgggttca ggctgtttgg tgcaacgtcg tagaaggctt cgtcagcaac
    11941 aacaacgtca taatcaatat tcgctgaacc aacccatttg gtgttgttca gagtcaggtt
    12001 caggtgatcg acgttctgat cgtcagttgc ccaaccgcca tgagtgtaag cagtggtaga
    12061 gttgtcaatc aggtgacccg tcgcgtcaaa gttagcgttc caggtactgt caaatacaac
    12121 gtcaccaagc aactgagagt tgttgaacgt aacgttgttg accatacgat aatcagaagt
    12181 gtcatcatcg atgaaggcta acgcaacatc atcagcagga gatgcgccat tgccgtagtc
    12241 acttggttca acagaattgc cgaaatgacc acgatctgat tgttcgtctt cagtccatga
    12301 accagaagtt acagtagagt cattaacaac gatgttgttg tcccactgat gaccttcggt
    12361 attgtaggtc gcattaccga ggtcgtgttg aaccaaagta atacctgcaa catgggagtt
    12421 gttgctgatg ttgatgttac tttcaacatc cagagtcaca gcggtaccaa gaccagcggt
    12481 taccaaacga tcataatctg tcttagatgt tttaccgtct gcatccagat aagtatcggt
    12541 gaagtagaag gcgtcaaaat catcgtcaat agtagaatta gcgatgttca gagtgaagat
    12601 gtcgccatca acccagtcgt ggccagtata atcgctaccg ttggtccaga cgtagtcgcc
    12661 ataaccaacc tggtaagatg taaccatacc atgaacaatt gagttggaga tgttcagtgt
    12721 attaccggta tctgtgccat aatcgtggtt cagataataa gtagaaatta caccatttac
    12781 agtgtcgccg ctgaaagtgg tatcaaagta accatcagct gcgtggtcat atacataacc
    12841 accgtaagtc agataatcgt gatcaatttt agtgctgtca ccccaattgt agatggtggc
    12901 tgcgtcatcg tgatacccga aggtgttata agtagctgca ttagcctgag aggtgatagc
    12961 cagagtgcaa gccaatgcta attgtgatac tacaagtttc tttttccagg agtgcatttg
    13021 tcatccctcc tcagggacgt aataagttga tccatcaatt atcaatgcat agaaaacata
    13081 ttatgtttcg cgctaacgat tatgctgcgt tacagacgaa tggttcaatt attctttccc
    13141 ctaagtccga atgtggacat atataattta tggagaaaaa cacgcatcaa attgataaaa
    13201 ggacgatatt tcaacatgtt gcagataatg ttatttttga tacatacgcg ttataaatat
    13261 caaaaacaca aaccagataa atatcttatc aatatacaac aaagtttgaa ttattcaatt
    13321 tatagcagca caaccaaacg ccaaaaaaaa taataaccaa gagctataaa taaagattaa
    13381 aaatattttc agtgaatatg aaattattgc tatttctatt acaaagatgt cctggtctct
    13441 ctttcattct cttcttgtca acatcacgac aactttcgta aaacactcct gccccgcttc
    13501 aggtatactg tgcaccttaa ttccacaaac atcaggcaga ccatgacaga cttaatccaa
    13561 cgccctcgtc gcctacgcaa atctcctgcg ctgcgcgcta tgtttgaaga gacaacactt
    13621 agccttaacg acctggtgtt gccgatcttt gttgaagaag aaattgacga ctacaaagcc
    13681 gttgaagcca tgccaggcgt gatgcgcatt ccagagaaac atctggcacg cgaaattgaa
    13741 cgcatcgcca acgccggtat ccgttccgta atgacttttg gcatctctca ccataccgat
    13801 gaaaccggca gcgacgcctg gcgggaagat ggactggtgg cgcgtatgtc gcgcatctgc
    13861 aagcagactg tgccagaaat gatcgtcatg tcagacactt gtttctgtga atacacttct
    13921 cacggtcact gcggtgtgct gtgcgagcat ggcgtcgaca acgacgcgac tctggaaaat
    13981 ttaggcaagc aagccgtggt tgcagctgct gcaggcgcag acttcatcgc cccttccgcc
    14041 gcgatggacg gccaggtaca ggcgattcgt caggcgctgg acgctgcagg ctttaaagat
    14101 acggcgatta tgtcgtattc gaccaagttc gcctcttcct tttatggccc gttccgtgaa
    14161 gctgccggaa gcgcattaaa aggcgaccgc aaaagctatc agatgaaccc aatgaaccgt
    14221 cgcgaggcga ttcgtgaatc actgctggat gaagcccagg gcgcagactg cctgatggtt
    14281 aaacctgctg gagcgtacct cgacatcgtg cgtgagctgc gtgaacgtac tgaattgccg
    14341 attggcgcgt atcaggtgag cggtgagtac gcgatgatta agttcgccgc gctggcgggt
    14401 gctatagatg aagagaaagt cgtgctcgaa agcttaggtt caattaagcg tgcgggtgcg
    14461 gatctgattt tcagctactt tgcgctggat ttggctgaga agaagattct gcgttaattt
    14521 ttttcctctc agaaaggggg agttaacctg ttgaaagtac ggtgaacgcc ccctcaccct
    14581 aaccctctcc ccaagggggc gagggaaccg tccgcactcg tatcaccccg cccgataaaa
    14641 cggtttatcc ccaaggatcg tcgcccgatg cataatccgc cgctgtggca ggtaatcggc
    14701 attggcatag tgctgagtca cgcggttatc ccaaatcgca atatcattcg gttgccagcg
    14761 ccaacgcacc tgaaactccg gtttagtgat atgggcgaat aaaaagccta acaaggcttc
    14821 gctctctttc tcgctgacat cgacaattcg cgtagtaaag ccttcattca caaacagcgc
    14881 ctgtttaccg ctcaccggat gcgttcgcac caccggatgt agcaacggag gatttttcgc
    14941 gaccgcctcg cgccagcgtt gatgttcctc ctcggttttg cggtatttgt attccgggaa
    15001 cgatttacgg aaatcatgct ccgcacgcag cccgctcagc agctggcgga agggaacaga
    15061 gagcgcctca taggccgcaa taccgctggt ccagagcgta tcaccgccgg tcgaaggtaa
    15121 ctctttagct gccagaatcg cccctgcggg tggcgtttca ataaatgtca catcggtatg
    15181 ccagttgtcg ttatccggcg gattatcgtt atgggtatcc agcacgatga tctcgtcaac
    15241 cccttcggca tgagggtaaa cagggtgaat atgcagttcg ccaaaacgct gggccagcgc
    15301 gcgttgctgc tgcggcgtaa tagcttgatc gcgcagaaat accacctgat agcgcagcac
    15361 cgcatggtaa agctgttcaa actgattatc gcttaacggg cgcgtcaggt cggcacccga
    15421 aatttgtgcg ccgatatacg gccccagcgg ggtaatactc agacgttcac tcattgtact
    15481 tctccatgcc agggcgtcag acggcgctgt aacgcgcgca gacccagttc taaaagaaag
    15541 gcgataatcg caatcaccgc gatccccgcc agtaccacgt cagtcgcgag aaattcaccc
    15601 gctgactgaa ccataaatcc taaaccgcgt gtcgcggcaa tcagctccgc cgctaccagc
    15661 gtagaccagc ccacacccag accaatgcgt aatccggtga ggatttccgg cagcgcgccg
    15721 ggcaaaatga caaaccacag cacctgcgca cggctggcac ccagcgactg ggcggcacga
    15781 atgcgaacct gctgcgcgct tttcaccccc gccagcaccg acatcgccac cggtgcaaaa
    15841 atcgctaaat agatcaataa gatcttcgag gtttcaccaa taccaaacca gatcaccatc
    15901 agcggcaaat aagccagcgg cggcaccgga cgataaagtt cgattaccgg atccagaatg
    15961 ccgcgcaccg tcgggcttag ccccatggcg atcccgaccg gaataccgat caccaccgcc
    16021 gcgagtagcg ccagcacaat gcgcgtcagg ctggctgcca gatgctgcca cagcgtggcg
    16081 tccataaatc cctgcggtcc ggcaatggtg agcagttttg ccagtacttg ttgcggcggc
    16141 ggcaaaaaca gcgggctaat cagttgcagc gccgccaccg tccaccatac agtgagtaaa
    16201 accgctaacg tgccaatgct taaggtcacc tgacgcgaga gcggccagcg ccatttcagc
    16261 cgccgcgaat gcagtttttc attaatgagc acactcatga gaacgcctcc cgttgctcaa
    16321 atacgcggct taaaacatat tcgcgcatgg cgatgaattg tggatcggac ttgatgctgc
    16381 ggctcgactc tcccgcaaca aagcggcgag caaagttgag cggcagccgc tccagcacac
    16441 ggccagggcc ggatgaaagc agaaccagtt cagtcgccat aaacaccgct tcttctatat
    16501 cgtgggtaat caacagcacc tgcttgcccg tctcctgcca gagtttcagc agcagagttt
    16561 gcatctggtc gcgggtgaag gcgtccagcg caccaaacgg ttcgtcgagt aataacagct
    16621 ggggattcgc cgccagcgcg cgggcaatcc ccacccgctg acgttgacca ccggaaagct
    16681 gccagatata gcgtttttct gcgccttcca gccccacttt tttcagcatc tggtgcgcga
    16741 tttccagtcg ctgcattttc tctatacctg ccaattgcag gccgaacgcc acgttgtcct
    16801 gtacattgcg ccacggtagt agcccttcat tctgaaaaac caccccgcgc tctgctcccg
    16861 gtccctcaat acgcttaccc gccagttgaa tgctgccatg ctgataaggc acaaaaccgg
    16921 caatcagatt cagcagggtg gttttaccgc agccggacgg ccccagcacc accagtagct
    16981 cgccgctttc cagcgtcagg ttgatatctt ccagtgccgg tttgccgcca taatcggcat
    17041 agagatgaga gatttgcagc atctgcgcct ccttttattg cacgaagcgc gaggtaacgt
    17101 actggctgta atcattcgct acagccggga ccttgccctg ctctttcaaa aactgcgcgg
    17161 tgtcgatgat tgctttgttc accggtccgg tcagttctgc cgtttgttgc tgcggcgtca
    17221 gataggtatt ccccttcacc agccccggaa cgtcaccttc cggcacgccg cttaaacgcg
    17281 ccagtttgct gatgttttcc ggctgtttca gccacgcgtc tgggttagca atgtacggtt
    17341 gctgagcatc gatggcgctt ttagcgaacg ctttcacgac ctcaggatgt ttctcggcaa
    17401 aatctttgcg caccacccag acgtccagcg ttggcgcacc ccactgcccg acctgttcag
    17461 aatcggtcaa caccttgccg tctttttcca gggcgttaac cgccggtgcc cagacataag
    17521 caccatcaat atcaccccgc tgccatgcgg cgataatcgc gggcggctgc aggttcacaa
    17581 tctccacttg ccctggttta atcccccagt gtttcagcgc cgccagcagg ctgtagtggg
    17641 tggtggagat aaacggtacg gcaatgcgtt tgccaatcaa atcttcgggt ttgctgatag
    17701 ttttctttac caccagcgct tcggagttac ccagttttga cgccagcaag aagacttcaa
    17761 tcggcacctg ttggctggct gcaaccgcta atgggctgga accgaggttg ccgatttgca
    17821 cgtcgccaga agccagcgcc cgcacgatgc tggctccgct gtcaaactta cgccagtcca
    17881 cgctcgcccc gctctcttta gcaaaggtgt tgtcggcctg agccactttc gccggttcgg
    17941 ctgaggtttg atacgccacg gtgacgttca ccgcctgtgc ctgaaaagcg atgaatgcca
    18001 gtgcggcaag aagtgtgtta cgcgatgaaa ttgccatgat tgtctgctcc cctgtcttgt
    18061 tatgggagca gtattcagga ataaaaacat tcattaaaag aattagtcgt tatcgcacag
    18121 atgattttat tcttagcaaa aaaacggcaa aggaaagatt
//
LOCUS       DAJTYZ010000083        17959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000083 DAJTYZ010000000
VERSION     DAJTYZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.083"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(229..1203)
                     /gene="cysB"
                     /locus_tag="OO596_002695"
     CDS             complement(229..1203)
                     /gene="cysB"
                     /locus_tag="OO596_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6718517.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1413..4010)
                     /gene="topA"
                     /locus_tag="OO596_002696"
     CDS             complement(1413..4010)
                     /gene="topA"
                     /locus_tag="OO596_002696"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6718518.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4390..4641
                     /locus_tag="OO596_002697"
     CDS             4390..4641
                     /locus_tag="OO596_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6718519.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="OO596_002698"
     CDS             complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="OO596_002698"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6718520.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5946..6704
                     /locus_tag="OO596_002699"
     CDS             5946..6704
                     /locus_tag="OO596_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6718521.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6701..7291
                     /gene="cobO"
                     /locus_tag="OO596_002700"
     CDS             6701..7291
                     /gene="cobO"
                     /locus_tag="OO596_002700"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6718522.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="OO596_002701"
     CDS             complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="OO596_002701"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6718523.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8416..9530)
                     /locus_tag="OO596_002702"
                     /pseudo
     CDS             complement(8416..9530)
                     /locus_tag="OO596_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9558..10178)
                     /locus_tag="OO596_002703"
     CDS             complement(9558..10178)
                     /locus_tag="OO596_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6718524.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10175..11056)
                     /gene="yciV"
                     /locus_tag="OO596_002704"
     CDS             complement(10175..11056)
                     /gene="yciV"
                     /locus_tag="OO596_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6718525.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11194..11238
                     /gene="trpL"
                     /locus_tag="OO596_002705"
     CDS             11194..11238
                     /gene="trpL"
                     /locus_tag="OO596_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6718526.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11215..11308
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11330..12892
                     /gene="trpE"
                     /locus_tag="OO596_002706"
     CDS             11330..12892
                     /gene="trpE"
                     /locus_tag="OO596_002706"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6718527.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12892..14487
                     /gene="trpD"
                     /locus_tag="OO596_002707"
     CDS             12892..14487
                     /gene="trpD"
                     /locus_tag="OO596_002707"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6718528.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14488..15849
                     /gene="trpCF"
                     /locus_tag="OO596_002708"
     CDS             14488..15849
                     /gene="trpCF"
                     /locus_tag="OO596_002708"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6718529.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15861..17054
                     /gene="trpB"
                     /locus_tag="OO596_002709"
     CDS             15861..17054
                     /gene="trpB"
                     /locus_tag="OO596_002709"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6718530.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17054..17860
                     /gene="trpA"
                     /locus_tag="OO596_002710"
     CDS             17054..17860
                     /gene="trpA"
                     /locus_tag="OO596_002710"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6718531.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4315 a         4750 c         4488 g         4406 t
ORIGIN      
        1 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
       61 tttgaaaact ggagtttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
      121 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
      181 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
      241 cagttttata tctttaaaca tgacctcaat ttcttcatta gagcgcaatg cgacagccgc
      301 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
      361 gcgcaagaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
      421 agcatcaaca cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
      481 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
      541 gacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
      601 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
      661 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
      721 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagtga aatcagcatt
      781 gcctttagag acggcatcag caatttgtgt cggcgagccc tggtgcatat gcaaagaaac
      841 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
      901 atgcgtggtg gcgatataca gtgagccttt atccggccag gtgtgctctc cggcaaccga
      961 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgccctgc
     1021 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
     1081 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
     1141 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
     1201 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
     1261 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
     1321 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
     1381 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
     1441 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
     1501 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
     1561 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
     1621 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaaaacaccc
     1681 gcagcgccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
     1741 ggcaccggat cttctttcgg tggtgccact tcgccgttac gtaaaatctt acgtgtgttt
     1801 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
     1861 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
     1921 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
     1981 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
     2041 cgcagcgcgt tggtttcagc gtcttcgcct tccagtacgt tcagcacttc gttttccggc
     2101 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcataacc agaacagcca
     2161 aggaataccc cggtgctcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
     2221 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
     2281 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
     2341 tcgtgatttg ccaccaggtc gaggctgttt tccatctgtg cggtgaagtc gtagttcatt
     2401 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgccaatttt ttccgcatag
     2461 aaacgacgat tttctactcg cacgtagcca cgatcctgaa tggtcgaaat gatcgacgca
     2521 taggtagacg gacgaccgat accgcgtttt tccagctctt taaccagcga tgcttcactg
     2581 aaacgggctg gcggcttggt aaagtgctgg gctggtgtaa gttcaacgag cgtcagagca
     2641 tcgcctttat taactgctgg taagatgcga tcttcatcgc ctttacgcaa cgcaggcatc
     2701 acttttgtcc agccatcaaa acgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
     2761 gcaccaatgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggctacgaac
     2821 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
     2881 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
     2941 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
     3001 ccacgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
     3061 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaatcca
     3121 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
     3181 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
     3241 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcgttctg atgagtcacc
     3301 tgtaacgcca acgcttcacc agatggcgtg gtcgtgctgg catcgacttc ccagaactct
     3361 tccggcacga acgctttaat ttcacgctca cgctcgacca ccaggcgaac cgccaccgac
     3421 tgcacacgac cggcagacag accacgagcg atctttttcc atagcagcgg cgaaaccata
     3481 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
     3541 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
     3601 actcggctat agcgcgcatc atcaccccca atcacttccc gcaggtgcca tgcaatggct
     3661 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
     3721 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
     3781 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
     3841 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
     3901 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cactacccag atacttgttg
     3961 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
     4021 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
     4081 taccgatatg gactcgttct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
     4141 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
     4201 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
     4261 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
     4321 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
     4381 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
     4441 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
     4501 cagcgtaatg gcgtgctggt atttatcggc gactactttt tagatgaaca aggattgcca
     4561 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
     4621 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
     4681 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
     4741 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
     4801 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
     4861 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
     4921 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
     4981 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
     5041 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
     5101 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
     5161 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
     5221 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
     5281 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
     5341 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
     5401 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
     5461 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
     5521 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
     5581 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
     5641 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt cgccaaaaac
     5701 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
     5761 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
     5821 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
     5881 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
     5941 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
     6001 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
     6061 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
     6121 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
     6181 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
     6241 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
     6301 acgtcatgca ggtcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
     6361 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttggg cgtcagggac
     6421 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
     6481 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
     6541 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
     6601 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
     6661 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
     6721 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
     6781 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
     6841 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
     6901 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
     6961 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
     7021 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
     7081 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
     7141 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
     7201 tcttgaactg gcggatacgg taagtgagct gcgccccatc aaacatgcgt ttgatgccgg
     7261 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
     7321 catactttga ttaaccgtta ttacgcccgc cagaacgacg accgccgctc acctgactgt
     7381 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
     7441 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
     7501 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
     7561 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
     7621 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
     7681 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
     7741 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
     7801 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
     7861 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
     7921 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
     7981 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
     8041 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
     8101 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
     8161 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
     8221 tcgccttccc aggcgtctga tatgcttttg tgcaacagtg agtcagaact tacatctgac
     8281 tcactgatag taaagtctgt ttgaactctt caggccttaa gtaaacgact cgacgtttta
     8341 actggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga
     8401 ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcgccgc caccagcacc
     8461 accaccggaa taacttcgcc ccaacaaact ggttttggcc cccagactca ggtgcatctg
     8521 taatgaaaac aaaacgccac tacgggcaat ttccggggcg ctaacagccg ctgtgagtga
     8581 tggggtgata cgtgctaccc attctcttcc aaggtttgct gagactgccc aacctagcaa
     8641 cgcgtaatcc agtttccgtg tttccccttt ccgccgacgc ccactgtgtg ttgctgcctc
     8701 tttacgcgca agatagttaa cgatagcttc accgtgggca taacgttgct gcccagtttg
     8761 tgtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca ggaaaacagc
     8821 gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat tgatgtagag
     8881 agaatagaag gccacgcctg aacaaatcag tggtaaacaa ttcttccgtc ctcaaggatg
     8941 cggccatttc gatatgtgtc gccctcttct ccagtaaaga gatcgatttc gctaaatgga
     9001 ttacccgcga tatcagggaa cttgagtgaa aatcgggtct tatagatttc aaacggccag
     9061 tggttaccag tcacgttcca gattaacaga tctgaatctc cctcacgcag gaaagcattt
     9121 ttaacctgat aatgaatttg ataatgatag tcgccttgag gcaagaaacg ttgtttatcg
     9181 cccacgacaa tactcattaa cccgatatgc cagtcatgat gccagggttc aggaatacca
     9241 tcacgggtga cgccaacaat atgatagttc agcagtgcag cgtccccacc aggctgcatc
     9301 cagagtcgtg gtaaatcacg ataaaatccg tgtttaatct gctgatttcg cgcctgaaaa
     9361 tgaatatttt ctttgatgtc gagcacccca tccggttgca gagtggcttc aacatcaaag
     9421 agcgaaatcc cttcttcata agaagggatc tccgtgtttt ctgcataact tagagatgat
     9481 acaaatagcc ctgctatcaa cagcaaaatg cagcgaaact tccctgccat agtcttccat
     9541 ccgtggaacg caatttgtta taagaaaggc ttcacatcac ctacgccttc acgcaccacg
     9601 accggcgtat catcggtaag atcaataacc gttgtcggtt tctgcccgag atagccgccg
     9661 tgaataatca aatctacctg tttttccaga cggtctttaa tttcttcggg atcggattcg
     9721 gtaaattcgc tgcctggcag cattagcgaa gtggaaagca tcggttcgcc cagtgcttca
     9781 agcaacgcct gcgcaatcgg gttagaaggc acacgcatac cgatggtttt gcgtttttcc
     9841 tgcaacagac ggcgtggcac ttccttcgtc cccttcagga tgaaggtata gttgcccggc
     9901 gtgttgtttt tcattaaacg aaacgcaacg ttatcaacaa atgaataggt cgagagttca
     9961 gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg gcagctggcg aatacgacaa
    10021 atacgctcca tcgcgttttt atcttcaatt ttacagccga gcgcatagcc ggaatcagtt
    10081 ggataaacaa tcaccccgcc tttacgcacg atctccaccg cctggttgat cagacgttgc
    10141 tgtgggttat caggatgaat ataaaaaaac tggctcataa ttccctctct gtggtgttct
    10201 gcggctgttc ccatagctgc cagacgcctt caacgcctgc gggcagccag agtttacgac
    10261 ctagttcaat ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc cataaatgat
    10321 gctgacgcgc aagggccgcc agctgggtac gttcattggg cgattgctgg cactgcgcga
    10381 cttccatcgc gtcaccgtgg tgttcggcaa aatgcgcaac caatcttttc agccatttag
    10441 cactaagatt gtaccgccca ggatgagcta gcaccgcctt accgccagaa tgatgaatga
    10501 catcaatagc ttgttctatt gtacaccact gtggcggaac gtatccggtt ttcccgcgcg
    10561 ccagatattt tttaaagaca tccgccattg aactggcttt gccgcacccc acaaggaaac
    10621 gcgcaaaatg accgcgcgtc accgcaccgc cctgcgccag tcgtcgcgcg ccttccagtg
    10681 cccctggtat ttgcgctttt tcaagtcttt cggcaatcag ttgcgcccgc tgattgcgcc
    10741 gttctgtctg ctgcgcgagg aactcacaca ttagcggatg agtaatatca atattcagcc
    10801 cgacaatatg aatttcatga ttttcccaga ccgtggaaat ttccacgccg ggaataagat
    10861 tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg cacgattgca gctgtgttgt
    10921 catggtcggt gatcgccagg gtaccgacgc gcatctcgac tgcacggtgc accaatactt
    10981 ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcgt
    11041 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
    11101 acagttctgg caaatattct gaaatcagct gttgacaatt aatcatcgaa ctagttaact
    11161 agtgcgcaag ttcacgtaaa aagggtatcg acaatgaaag caattttcgt actgaaagct
    11221 tggtggcgca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa tcagataccc
    11281 agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa tgcaaacaca
    11341 aaaaccgact ctcgaactgc taacctgcga aggcgcttat cgcgacaacc cgactgcgct
    11401 ttttcaccag ttgtgtggga atcgtccggc aacgctgctg ctggaatccg cagatatcga
    11461 cagcaaagat gatttaaaaa gcctgctact ggtagacagt gcgctgcgca ttacagcatt
    11521 aggtgacact gtcacaattc aggcactttc cggcaacggc gaagccctgc tggcactact
    11581 ggataacgcc ctgcctgcgg gtgtggaaaa tgaacaatca ccaaactgcc gtgtgctgcg
    11641 cttcccccct gtcagtccac tgctggatga agacgcccgc ttatgctccc tttcggtttt
    11701 tgacgctttc cgtttattgc agaatctgtt gaatgtaccg aaggaagaac gagaagcaat
    11761 gttcttcggc ggcctgttct cttatgacct tgtggcggga tttgaagatt taccgcaact
    11821 gtcagcggaa aataactgcc ctgatttctg tttttatctc gctgaaacgc tgatggtgat
    11881 tgaccatcag aaaaaaaaca cccgcattca ggccagcctg tttgctccga atgaagaaga
    11941 aaaacaacgt ctcactgctc gcctgaacga tcttcgccag cagctgaccg aagccgcgcc
    12001 gccgctgccg gtggtttccg tgccgcatat gcgttgtgaa tgtaatcaga gcgatgaaga
    12061 gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc gctggagaaa ttttccaggt
    12121 ggtgccatct cgccgtttct ctctgccctg cccgtcaccg ctggcggcct attacgtgct
    12181 gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag gataatgatt tcaccctgtt
    12241 tggcgcgtcg ccggaaagtt cgctcaagta tgacgccacc agccgccaga ttgagatcta
    12301 cccgattgcc ggaacacgtc cacgcggtcg tcgtgccgat ggctcgctgg acagagacct
    12361 cgacagccgc atcgaactgg aaatgcgtac cgatcataaa gagctttctg aacatctgat
    12421 gctggtggat ctcgcccgta atgatctggc acgcatttgc acccccggca gcagctacgt
    12481 cgccgatctt accaaagttg accgttactc ttacgtgatg cacctggtct cccgcgtggt
    12541 cggtgagctg cgccacgatc tcgacgccct gcacgcttac cgcgcctgta tgaatatggg
    12601 gacgttaagc ggtgcgccga aagtacgcgc catgcagtta attgccgagg cggaaggtcg
    12661 tcgccgcggc agctacggcg gcgcggtagg ttatttcacc gcgcatggcg atctcgacac
    12721 ctgcattgtg atccgctcgg cgctggtgga aaacggtatc gccaccgtgc aagccggtgc
    12781 tggcgtagtc cttgattcta ttccgcagtc ggaagccgac gaaacccgta ataaagcccg
    12841 cgctgtactg cgcgctattg ccaccgcgca tcatgcacag gagactttct gatggctgac
    12901 attctgctgc tcgataatat cgactctttt acgtacaacc tggcagatca gttgcgcagc
    12961 aatgggcata atgtggtgat ttaccgcaac catattccgg cgcagacctt aattgaacgc
    13021 ctggcgacga tgagcaatcc gatactgatg ctttctcctg gccccggtgt gccgagcgaa
    13081 gccggttgta tgccggaact cctcacccgc ttgcgtggca agctgcccat tattggcatt
    13141 tgcctcggac atcaggcaat tgtcgaagct tacgggggct atgtcggtca ggcgggcgaa
    13201 attcttcacg gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt tgccggatta
    13261 acaaacccgc tgccggtggc gcgttatcac tcgctggttg gcagtaacat tccggccggt
    13321 ttaaccatca acgcccattt taatggcatg gtgatggcag tacgtcacga tgcggatcgc
    13381 gtttgtggat tccagttcca tccggaatcc attctcacca cccagggcgc tcgcctgctg
    13441 gaacaaacgc tggcctgggc gcagcagaaa ctagagccag ccaacacgct gcaaccgatt
    13501 ctggaaaaac tgtatcaggc gcagacgctt agccaacaag aaagccacca gctgttttca
    13561 gcggtggtgc gtggcgagct gaagccggaa caactggcgg cggcgctggt gagcatgaaa
    13621 attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa ccgcgctact ggaaaacgca
    13681 gcgccgttcc cgcgcccaga ttatctgttt gctgatatcg tcggtactgg cggtgacggc
    13741 agcaacagta tcaatatttc taccgccagt gcgtttgtcg ccgctgcctg tgggctgaaa
    13801 gtggcgaaac acggcaaccg tagcgtctcc agtaaatctg gttcgtccga tctgctggcg
    13861 gcgttcggta ttaatcttga tatgaacgcc gataaatcgc gccaggcgct ggatgagtta
    13921 ggtgtttgtt tcctctttgc gccgaagtat cacaccggat tccgccacgc gatgccggtt
    13981 cgccagcaac tgaaaacccg caccctgttc aatgtgctgg ggccattgat taacccggcg
    14041 catccgccgc tggcgttaat tggtgtttat agtccggaac tggtgctgcc gattgccgaa
    14101 accttgcgcg tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg gatggatgaa
    14161 gtttcattac acgcgccgac aatcgttgcc gagctgcatg acggcgaaat taagagctat
    14221 caattgaccg ctgaagattt tggcctgact ccctaccacc aggagcaact ggcaggcgga
    14281 acaccggaag aaaaccgtga cattttaaca cgcttgttac aaggtaaagg cgacgccgcc
    14341 catgaagcag ccgtcgctgc gaacgtcgcc atgttaatgc gcctgcatgg ccatgaagat
    14401 ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca gtggttccgc ttacgacaga
    14461 gttaccgcgc tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa aatcgtcgca
    14521 gacaaggcga tttgggtaga aacccgcaaa cagcagcaac cgctggccag ttttcagaat
    14581 gaggttcagc cgagcacgcg acatttttat gatgcgctac agggtgcacg cacggcgttt
    14641 attctggagt gcaagaaagc gtcgccgtca aaaggcgtga tccgtgatga tttcgatccg
    14701 gcacgcattg cctccattta taaacattac gcttcggcaa tttcggtgct gactgatgag
    14761 aaatattttc aggggagctt tgatttcctc cccatcgtca gccaaatcgc cccgcagccg
    14821 attttatgta aagacttcat tatcgaccct taccagatct atctggcgcg ctattaccag
    14881 gccgatgcct gcttattaat gctttcagta ctggatgacg accaatatcg ccagcttgca
    14941 gccgtcgctc acagtctgga gatgggggtg ctgaccgaag tcagtaatga agaggaactg
    15001 gagcgcgcca ttgcattggg ggcaaaggtc gttggcatca acaaccgcga tctgcgtgat
    15061 ttgtcgattg atctcaaccg tacccgcgag cttgcgccga aactggggca caacgtgacg
    15121 gtaatcagcg aatccggcat caatacttac gctcaggtgc gcgagttaag ccacttcgct
    15181 aacggttttc tgattggttc ggcgttgatg gcccatgacg atttgcacgc cgccgtgcgc
    15241 cgggtgttgc tgggtgagaa taaagtatgt ggtctgacgc gtgggcaaga tgctaaagca
    15301 gcttatgacg cgggcgcgat ttacggtggg ttgattttgg ttgcgacatc accgcgttgc
    15361 gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac cgttgcagta tgttggcgtg
    15421 ttccgcaatc acgatattgc cgatgtggtg gacaaagcta aggtgttatc gctggcggca
    15481 gtgcaactgc atggtaatga agatcagctg tatatcgata cgctgcgtga agctctgcca
    15541 gcacacgttg ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc ccgcgagctt
    15601 cagcacgttg ataaatatgt tttagacaac ggccagggtg gaagcgggca acgtttcgac
    15661 tggtcactat taaatggtca atcgcttggc aacgttctgc tggcgggggg cttaggcgca
    15721 gataactgcg tggaagcggc acaaaccggc tgcgccggac ttgattttaa ttctgctgta
    15781 gagtcgcaac cgggcatcaa agacgcacgt cttttggcct cggttttcca gacgctgcgc
    15841 gcatattaag gaaaggaaca atgacaacat tacttaaccc ctattttggt gagtttggcg
    15901 gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa gcttttgtca
    15961 gcgcgcaaaa agatcctgaa tttcaggccc agttcaacga cctactgaaa aactatgccg
    16021 ggcgtccaac cgcactgacc aaatgccaga acattacggc tggcacgaac accacgctgt
    16081 atctgaagcg cgaagatttg ctgcacggcg gcgcgcataa aactaaccag gtgctcggtc
    16141 aggctttact ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc
    16201 agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc cgtatttata
    16261 tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg
    16321 cggaagtgat cccggtgcat agcggttccg cgacgctgaa agatgcctgt aacgaggcgc
    16381 tgcgcgactg gtccggtagt tacgaaaccg cgcactatat gctgggcacc gcagctggcc
    16441 cgcatcctta tccgaccatt gtgcgtgagt ttcagcggat gattggcgaa gaaaccaaag
    16501 cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg
    16561 gttcgaatgc catcggcatg tttgctgatt tcatcaatga aaccaacgtc ggcctgattg
    16621 gtgtggagcc aggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg
    16681 gtcgcgtggg tatctatttc ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga
    16741 ttgaagaatc ttactccatc tccgccggat tggatttccc gtctgtcggc ccacaacacg
    16801 cgtatcttaa cagcactgga cgcgctgatt acgtgtctat taccgatgat gaagcccttg
    16861 aagccttcaa gacgctgtgc ctacacgaag ggatcatccc ggcgctggaa tcctcccacg
    16921 ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga aaaagagcag ctactggtgg
    16981 ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt ttgaaagcac
    17041 gaggggaaat ctgatggaac gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga
    17101 aggcgcattc gttcctttcg tcacgctcgg ggatccgggc attgagcagt cattgaaaat
    17161 tatcgatacg ctaattgaag ccggtgctga cgcgctggag ttaggcatcc ccttctccga
    17221 cccactggca gatggcccga cgattcaaaa cgccactctg cgcgcctttg cggcaggtgt
    17281 gactccggca caatgttttg aaatgctggc actgattcgc cagaaacacc cgaccattcc
    17341 cattggcctg ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg agttttatgc
    17401 ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc gatgtgccaa ttgaagagtc
    17461 cgcgcccttc cgccaggccg cgttgcgcca taatgtcgca cctatcttca tctgcccgcc
    17521 aaatgccgat gacgacctgc tgcgccagat agcctcttac ggtcgtggtt acacctattt
    17581 gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc tcaatcatct
    17641 ggttgcgaag ctgaaagagt acaacgctgc acctccattg caggggtttg gtatttccac
    17701 cccggatcag gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt ctggttcagc
    17761 cattgttaaa atcatcgagc aacatattaa tgagccagag aaaatgctgg cggcactgaa
    17821 agtttttgta caaccgatga aagtggcgac gcgcagttaa tccccctgcc gccagttccg
    17881 ctggcggcat tttaactttc tttaacgaag ccggaaaaat cctaaattca tttaatattt
    17941 atctttttac cgtttcgct
//
LOCUS       DAJTYZ010000084        17891 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000084 DAJTYZ010000000
VERSION     DAJTYZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.084"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..771
                     /locus_tag="OO596_002711"
     CDS             <1..771
                     /locus_tag="OO596_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6718532.1"
                     /translation="QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSV
                     LSDMFREAIVEGRISQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMD
                     LALVTGQRREDITNMRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERC
                     RLVSRGDYLISAGIRKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAG
                     RLYKETCGEEFAQRLLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1164..1505)
                     /locus_tag="OO596_002712"
     CDS             complement(1164..1505)
                     /locus_tag="OO596_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6718533.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1518..2390)
                     /gene="copD"
                     /locus_tag="OO596_002713"
     CDS             complement(1518..2390)
                     /gene="copD"
                     /locus_tag="OO596_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCR6718534.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2394..2768)
                     /gene="yobA"
                     /locus_tag="OO596_002714"
     CDS             complement(2394..2768)
                     /gene="yobA"
                     /locus_tag="OO596_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6718535.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            2907..3137
                     /gene="holE"
                     /locus_tag="OO596_002715"
     CDS             2907..3137
                     /gene="holE"
                     /locus_tag="OO596_002715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6718536.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3239..3895
                     /locus_tag="OO596_002716"
     CDS             3239..3895
                     /locus_tag="OO596_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR6718537.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            3919..4581
                     /gene="exoX"
                     /locus_tag="OO596_002717"
     CDS             3919..4581
                     /gene="exoX"
                     /locus_tag="OO596_002717"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6718538.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4578..6638)
                     /gene="ptrB"
                     /locus_tag="OO596_002718"
     CDS             complement(4578..6638)
                     /gene="ptrB"
                     /locus_tag="OO596_002718"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6718539.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(6847..7506)
                     /locus_tag="OO596_002719"
     CDS             complement(6847..7506)
                     /locus_tag="OO596_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6718540.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(7833..8189)
                     /locus_tag="OO596_002720"
     CDS             complement(7833..8189)
                     /locus_tag="OO596_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6718541.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8256..8546)
                     /gene="yebG"
                     /locus_tag="OO596_002721"
     CDS             complement(8256..8546)
                     /gene="yebG"
                     /locus_tag="OO596_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCR6718542.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            8680..9858
                     /gene="purT"
                     /locus_tag="OO596_002722"
     CDS             8680..9858
                     /gene="purT"
                     /locus_tag="OO596_002722"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6718543.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(9914..10555)
                     /gene="kdgA"
                     /locus_tag="OO596_002723"
                     /gene_synonym="eda"
     CDS             complement(9914..10555)
                     /gene="kdgA"
                     /locus_tag="OO596_002723"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6718544.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10592..12403)
                     /gene="edd"
                     /locus_tag="OO596_002724"
     CDS             complement(10592..12403)
                     /gene="edd"
                     /locus_tag="OO596_002724"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6718545.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12638..14113)
                     /gene="zwf"
                     /locus_tag="OO596_002725"
     CDS             complement(12638..14113)
                     /gene="zwf"
                     /locus_tag="OO596_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6718546.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14451..15320
                     /gene="hexR"
                     /locus_tag="OO596_002726"
                     /gene_synonym="yebK"
     CDS             14451..15320
                     /gene="hexR"
                     /locus_tag="OO596_002726"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6718547.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRTVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15448..16890
                     /gene="pyk"
                     /locus_tag="OO596_002727"
     CDS             15448..16890
                     /gene="pyk"
                     /locus_tag="OO596_002727"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6718548.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(16950..17792)
                     /locus_tag="OO596_002728"
     CDS             complement(16950..17792)
                     /locus_tag="OO596_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR6718549.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4541 a         4518 c         4331 g         4501 t
ORIGIN      
        1 caactggaga caataaaaga aaaattggga gagattttac tgacagaaat aaccactcgc
       61 catattgccg agtttcttga tttgtggatt gaaggaggga aaaacacaat ggcaggatca
      121 atgcgttctg tgttgtctga tatgtttcgc gaggccattg ttgaaggacg tatatctcaa
      181 aatccagtca cgccaacaag agcaccgaaa atagtagtta caagagaacg actgaaacta
      241 aagatataca actgcatcag ggaggcagca gatcaacttc cggcatggtt cccattagct
      301 atggatttag cccttgtaac aggacaacgt cgcgaagaca taacgaatat gcggttcagt
      361 gatatttatg atgatcgtct ccacgtcagg caaattaaga caggaatgat gattgctatc
      421 cccctgtcac tcagccttcc tgtcgctggt ctacggcttg gtacagtagt tgaacgatgc
      481 cgcctggtaa gccggggaga ttatctaatc agtgccggga ttagaaaaaa cagccctgac
      541 ggcagcattc acccggatgg cctgacaaaa aaatttgtcg cagccagaaa attcacaggt
      601 atccagttca gtgaaaaccc accaactttt cacgagatca gaagcctggc tggacgattg
      661 tacaaagaaa catgtggaga agaatttgct cagcgtctac ttggccacac atcggagaag
      721 acaacaaaaa tgtatcttga tgagagagaa aaaacgtact tactgctctg attttaacgt
      781 aaatggattg ttaaatgtat tttggttgtg atataaccaa aaagaccgga atacagaaat
      841 tcgagtaaat ttcggggaat ttcgggggag acatttgcaa ctaattgatt tcaaatgcaa
      901 ttaaaaaaag accgaatacg attcctgtat tcggtccagg gaaatggctc ttgggagaga
      961 gccgtgcgct aaaagttggc attaatgcag gcttagttgc cttgcccttt aagaatagat
     1021 gacgacgcca ggttttccag tttgcgtgca aaatggtcaa taaaaagcgc ggtggtcatc
     1081 agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact gaggcggttt ttttattgga
     1141 aatcaaaagg ctattttagg taattaacag agtttttcag ctcgttctat aaacggtgcc
     1201 agactcattt tttcgccggg attgttagga tcatcaatct gaatcaccga aatgggttgg
     1261 gcattggtct tcccactggc aacttccttt tgtgcgatat cgtttaaagg atactgcacg
     1321 agggtactcg gattaataac atacaaagca ttacccggtc ggcaagtcag catcacctct
     1381 tcgcgattaa acgcccattt gtctttaccc acttcaaaac gactgacggt aatcacctgc
     1441 ggtgcagcca gcgccgctgc agaactggtg agtaacagaa acgccagaat actttttttc
     1501 atcattttcg taatccatca aaaaggttcg agagtcgcaa gcaggctgat gatcaccagc
     1561 actaccgcac ctatcgccca ttcgagttta gtcatccaca caaaccaggg tgccgcacga
     1621 tcttcctcct gcctcatgcg cggtacaaga acataacgat tagccagcgc aatgaccacc
     1681 ataatcatca ccaggatggc tttgagcaat aagagttggc cccagtaagt cgtaagcgtg
     1741 gggggaaatc cggtaatcaa caatgcatta agcacgccgc ttgccagtac gccgatcacc
     1801 gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa tagcctggtg tcgccaacgg
     1861 cctttgatga gttgcataca ccagagcacc ggcagtaatc ccccaaacca cgccgccgca
     1921 caaatcaggt gaatcgcgtg attagtctga tggattttcg ccgttacccc ttcattcagt
     1981 gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg tagtgagcat aaacagcaaa
     2041 cgtggcatat tccgcggttg cataagggcg acgatcaacg ttacgagggc gagaacaatt
     2101 tgccataacc agacgccacc aaactgcgtt tgcaaaacag cctgccaaat gtttggcgaa
     2161 aatacatctg tccatcccgt tcccatcaac ccgccctgaa cggcgagcat tgcagtggca
     2221 ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca gaaaacgctt cgccagcaag
     2281 cgacgaatcg tcaagggggc cagccaggca ccgtacattg cgaagccaaa aaccagcatc
     2341 agcgaggtga aatggataaa tcgcagcgcg atccaggtaa acgccagcat gatttatttc
     2401 acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca ccgaaacaac atgccagtca
     2461 acggtgtagg ttccgggttt cagcgaatcg gccagtggaa caatcagttg cttttggtcc
     2521 tgctcatttc gcttcgccgg taatgtttta atattttcgt tttttggccc cgtgattttt
     2581 gcaccactga atcctgtttc cacaccttcc gagaagttta aagtgattgc ctgcggtgca
     2641 gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta aatgcgcatg tgcccagacg
     2701 aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt agcgaaggga gcgtgcagtt
     2761 gaagccatat tatctattcc tttttgtaat aactttttta cagagcataa ccttgtctaa
     2821 tgtctgagtc gaggatcatc aattccggct tgccatcctg gctcactctt agtaactttt
     2881 gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct ggctaaactg gatcaaacag
     2941 aaatggataa agtgaatgtc gatttggcgg cggccggggt ggcatttaaa gaacgctaca
     3001 atatgccggt gatcgctgaa gcggttgaac gtgaacagcc tgaacatttg cgcagctggt
     3061 ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa tctgtcacgt ttaccttacg
     3121 agcccaaact taaataaaac ttatacaggg ttacactttc ttacataacg cctgctaaat
     3181 tatgagtatt ttctaaaccg cacccataat ttgcagtcat tttgaaaagg aagtcattat
     3241 gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt aaaacctcac tcaccgaaca
     3301 agtagctcat catctggagt ttgtgagggc cgccgccagg caacaatgtc agctgttagt
     3361 ttttccatcg ctttctttac tggggtgtga ttattcgcga cgtgcccttc ctgccccacc
     3421 cgatctctca ctgttagacc cgctttgtta tgccgcaacg acctggcgaa tgaccatcat
     3481 tgccggcctt cccgttgaat ataacgatcg ctttattcgt ggaattgcgg tgttcgcacc
     3541 gtggcgaaaa acgcctggga tctaccatca gagtcatggt gcatgtctgg gccgtcgttc
     3601 cagaaccatc acggtggtcg atgaacaacc ggaaggtatg gatatggacc cgacctgttc
     3661 actgtttacg acagggcaat gtctgggaga acccgacctg ctggcgtcgg cccgtcgctt
     3721 acaatttttt tcacatcagt actcgattgc tgtgctgatg gcaaatgccc gtggtaacag
     3781 tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc gatcgtggtt cattattgtt
     3841 agtcggtcag cgttcatcac agggttggca aggcgatatc attccattac gctaggcttt
     3901 ttcggcctgg agtatgccat gttgcgcatt atcgatacag aaacctgcgg tttgcaggga
     3961 gggatcgttg agattgcctc tgttgatgtc attgacggaa aaatcgtcaa ccccatgagc
     4021 cacctggtgc gccccgatcg tcctattagt ccacaagcga tggcgattca tcgcatcacc
     4081 gaagccatgg tcgccgataa accgtggatt gaagatgtga tcccacacta ttacggcagt
     4141 gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg tactgcctga gatgcccggt
     4201 gagtggattt gcaccatgaa actggcccgt cgcttgtggc caggaatcaa gtacagcaat
     4261 atggcgttat ataaaacacg gaagctcaat gtacagacgc cgccgggcct gcatcatcac
     4321 cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta tcgatattat gaacacctcc
     4381 ggctggacgg cagaacagat ggccgatatc accggacgtc cgtcgttgat gacgaccttc
     4441 acctttggca aataccgtgg caaagcggtt tccgacgttg ccgaacgcga tccgggctat
     4501 ctgcgctggt tatttaataa cctggacagc atgagcccgg agctgcgttt aacactgaaa
     4561 cattatctgg aaaatactta atccgcaggc gtagcgggta atgttccctg cgccagcgcg
     4621 accagaaaag catattccat cgctacgcct tcgtacgatt taaagcggcc agatttaccg
     4681 ccatggcctg agtccatgtc ggtacagagc aataaaagat ggttatcggt tttcagctcg
     4741 cgcaatttag cgacccattt tgccggttcc caatattgca cctgagaatc gtgtaaaccg
     4801 gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt tgtcatacgg gctgtagctt
     4861 ttcatgtact cgtaatattg cggatcctgc gggttacccc actcttcaaa ctcaccagtg
     4921 gtaagaggaa ttgattcatc aagcatcgtt gttacaacat caacaaacgg tacctgggcg
     4981 ataacgccgt ggaataattc cgggcgttga ttaattgcaa cgcccatcaa catgcccccc
     5041 gcactcccgc ccatcgcata acaaagcgaa ggagagccat agcccagttt taacaatgca
     5101 tcgcaggcat caagataatc attaaacgta tttttcttct tcagaaattt tccgtcttcg
     5161 taccattgtt gccccagctc accaccgccg cgaacatgga caatggcgta gacaaagcca
     5221 cgatctaaca aactcaagcg gctaaaactg aaatcggcat caatacttgc gccgtaagaa
     5281 ccatagccat acaccagcaa cgggttgtgt cctttgcgaa aatgtttgcg atggtagacc
     5341 aacgaaaccg gaacttcgac gccatcacgg gcgactatcc acaggtgttc actgcggtaa
     5401 ttcgccgcat caaaaccagg aacttccgtt tgttttaata cacgacgctc atcggtatcc
     5461 atatccagtt caaacaaagt gtctggtgtg gtcatggaag aataaccata acgcaatcgc
     5521 gcggtttcag gttctggatt gtaggcaatc caggtcacat aggctggatc atcaaaggca
     5581 ataccaatga cttcccgggt cttgcggtta atttggcgca aactggttaa cccgcgctga
     5641 cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc cttccagcat gatgttttcg
     5701 catggcggaa ttaactcttc ccactgttgc tcatcacgca tacgggtacg gtataagcca
     5761 aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat gctggtagtg atcaaggctg
     5821 tattcgtgat ctttgcggcg cggcagaaaa acaaacggct cggcatcggc catttccgcg
     5881 tccagcaggc gaacttcact ggtggtggcg ctggccaaat gaatgactac atagtgcttc
     5941 gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt tttcttcgta gatcagttta
     6001 tcttgcgatg ctggcgtacc gatggcgtga cgccagacct gataaggcag cagcgtcacc
     6061 ggatgcttgc gaacatagta gaaagtccag gagtcatttg cccagacaaa gctgggttca
     6121 acgttatcca gcagttccgg gtaccagtta ccagtttcca gattacgaaa acgaatgccg
     6181 tactggcgtc gggaaagaaa atcttctgcc agcgccataa tggtgttatc gggcgtaatc
     6241 gccattccgc ccatcgaata aaactcacta tgagctgcgc gcttattggc atcgagcaat
     6301 atttcccact catcccactc ttcactgaat gccgattgac gctggtagat agcatattca
     6361 cagcctggtt cataaatatg ccgatagcgg tagccatttt tgatgtaggg cgcagaaact
     6421 tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc gatcctgcaa ggcttgttgt
     6481 gaggccatca cccgatgacc gtaactattt tcttgttgca ggtagtccag gacttctggc
     6541 tgagaacgcg tatcatcccg cagccagtag taattatcga tgcgcgtatc gccatgaagc
     6601 gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt tattgttctt tctggttgaa
     6661 acatcttata agggtggcaa aactcaccgg ggatgcaagc gaaacagggg agtcattgct
     6721 tagatgatga caggtaatgg cacggatatc gaatgttatg cagacagaga aactcagcct
     6781 gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc aacttcaggc tatccagaga
     6841 gcggaattat tccgccaaag tgcgtttttg ctgttcgaga tcgcgttcaa tgccgtcacg
     6901 aacatcctgg gggattttca gcgcgtcacc cagtgcattc aggtaactgc gttccataaa
     6961 atggtcaata tcaatagccg cgcaactcag gaaatagatt tccagcgcct cttcttcatt
     7021 gcggaccccg gtagccaggc gttgtggatc cagcggttgt tcgattgcct gctcaatgag
     7081 tacacgcccc tgctcttcca cgccggcttc acgcaattgc tggtcgattg ccgcacgttc
     7141 tttggcatca atatgaccat cacttttagc ggcaaagacc agcgcaagga tcaaacgttc
     7201 tgtacgctca tccagcggcg tactttgcgc gccaaactgc ggttcgtcct gatgcgccgc
     7261 gcgaatttta tctttgtatt tattccacag caccgtaccc gctaccgctc cgccgccaac
     7321 cagtaacgcg ttggtgccat atttcgtaag aagtttacgt gctgatttat ttgcgaccag
     7381 cagcccagcc agtccgccta atgcgcctgg cactaacagt ttgcccaatc cctgatccgc
     7441 agacgaggag gtagaagaac tgctttgccc aagcagggat tgcagttgat ttaaccagtt
     7501 agccatattt gctcctcaat aaccattttt agtgcctgta gcatagcgga tgaggatgtc
     7561 agaaaatgta tatcgagaca aaagatgcgc aaataagcgg ggcggtaggc cgttattcaa
     7621 agaaattcgc gccaggtaag gtatttactg tatatcaggt gataagggta ttttaggtga
     7681 aagtagtttg tcgttttgag tggcattgtt tgatgctgat tgcccgatgc gacgctgttg
     7741 cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg acaaggcact cgcgccgcat
     7801 ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc gctgatattc cgccattccc
     7861 gctttgcagt ccacgctgac ctggtaatga atatcggcac ttttaccacg cacggttagc
     7921 ggtactgacc atttatcatc tttaccctga atgtcctgca aactgaccca agccacggga
     7981 tcggcctgac cgacaatttt ttgatcatct gcccaacgcg ccacgcgatt ttgttgataa
     8041 tcacgtttta cgctcgcggc aattccggca gcatccagac cttcacactt tgggaaagtg
     8101 accgacttgc tggtttcatt attggcagcg aaaactgatg cgcaggcaga aaccaacaac
     8161 agccctaaaa acgcccctct ttttttcatg tttttctcca tagcacaatg attcaggaga
     8221 aagcatggta caaattgtca ggagcgcaag ttgcttcagg ccgcgtgtga ggcatcttcc
     8281 tcttcggatt cagcaccgac gacctgtggg gacggtaatt tgccggtttt cagaatggtg
     8341 ctcagcacat ctttttgttc tgccagccat agcgaaaggg cttcacgttg ctcgtcttcc
     8401 atttgcactg gagaatttgt cagccaggtg tcaagcagat ccgccgtatc aagcattttg
     8461 tcataagcat cggcttcctt tttgctggta aacgacattt tctcttcgcc ctcacgaatg
     8521 actacgtatt taacttcaac cgccatttgc agcctctcat aataactgtg attttataca
     8581 gtatatttct tttcggttga gaaatcaaca tcagcaataa agacacacgc aaacgttttc
     8641 gtttatactg cgcgcggaat taatcagggg atattcgtta tgacgttatt aggcactgcg
     8701 ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg gtgaactggg taaagaagtg
     8761 gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg tcgatcgcta tgccgacgca
     8821 ccagccatgc atgtcgcgca tcgctcccat gtcattaata tgcttgatgg tgatgcatta
     8881 cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc cggagatcga agctattgcc
     8941 accgatatgc tgatccaact tgaagaggaa ggactgaatg ttgtcccctg cgctcgcgca
     9001 acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg cggcagaaga gctgcagctg
     9061 cccacttcca cttatcgttt tgccgatagc gaaagccttt tccgcgaggc ggttgctgac
     9121 attggctatc cctgcattgt aaaaccggtg atgagctctt ccggcaaggg gcagacgttt
     9181 attcgttctg cagagcaact tgctcaggca tggaagtacg ctcagcaagg cggtcgcgcc
     9241 ggagcaggcc gcgtaattgt tgaaggcgtc gttaagtttg acttcgaaat taccttgcta
     9301 accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg taggtcatcg ccaggaagat
     9361 ggcgactacc gtgaatcctg gcaaccacag caaatgagcc cgcttgccct tgaacgtgcg
     9421 caggagattg cccgtaaagt ggtgctggca ctgggcggtt atggtctgtt tggtgtcgag
     9481 ctgtttgtct gtggtgatga ggtgattttc agtgaggtct cccctcgtcc acatgatacc
     9541 gggatggtga cgttaatttc tcaagatctt tcagagtttg ccctgcatgt acgtgccttc
     9601 ctcggacttc cggttggcgg gatccgtcag tatggtcctg cagcttctgc cgttattctg
     9661 ccacaactga ccagtcagaa tgtcacgttt gataatgtgc agaatgccgt aggcgcagat
     9721 ttgcagattc gtttatttgg taagccggaa attgatggca gccgtcgtct gggtgttgcg
     9781 ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg cgaagcacgc cgccggacag
     9841 gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa aaatgcccga tcgtagatcg
     9901 ggcgttttga cttttacagc ttagcgcctt ctacagcttc acgcgccagc ttagtaatgc
     9961 ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag ccaggaacca ccgatgcaca
    10021 gcacgctttt cagcgccagg tagtcacggt agttagccgg agaaatacca cccgtcgggc
    10081 agaaacggac ctgggagaac ggacccgcga tcgcctgcag ggctttcacg ccgccgttag
    10141 cttcagccgg gaagaatttg aactctttca aaccgtagtc catacccagc atcagttcgg
    10201 aaacagtgct gatccccgga attagaggaa tagtcccttc ggtagcagct ttcagcagcg
    10261 gctcggtcag acccgggcta attgcgaact gtgcacccgc ttcagtgact tctgccagct
    10321 gctgtggatt cagcaccgta ccggcaccca caatcgcttc aggcacttct ttggcgatag
    10381 cacggatagc gtcaactgca cactcggtac gcagagtcac ttccagaacg cgcaccccac
    10441 cagcaaccaa cgcttttgcc atcggcactg cgtgttccag tttttttacc acgataaccg
    10501 gtacaaccgg gccggtggtc aggattgatt ctgcacttgt tttccagttt ttcatcagag
    10561 ttttctctcg cctgattaca aatttgtcgt cttaaaaagt gatacaggtt gcgccctgtt
    10621 cggcaccgga cagtttttca cgcaaggcgc tgaataattc acgtcctgtc cccacgcgtg
    10681 acgcgctcag gtcaggaatg tgcggttcgc gagcagccag ttccgcttcg tctaccagca
    10741 gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc cccgtcgcgc acttttgcca
    10801 gcagcccgcc atcgtaggct tctggtgtta cgtggatagc tgacggcact ttacctgaag
    10861 cgccggagag tcgtccatcg gtaactaacg caattttgaa acaccggtcc aataatacac
    10921 caagtggcgg catgagttta tgtaattctg gcattccgtt cgcttttggc ccctgatgac
    10981 ggacaacaac gacacaatcg cggtccagca aacccgcttc aaaggccggc ataacgtcat
    11041 gctggctttc aaaaacaacc gctggcgctt caatcacctg gttctcaacc ggcacggcag
    11101 aggttttcat aaccgcacgg cccaggttac cgcttaacac ttttgtccca ccatgatgag
    11161 agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag tgatttttcc gccccttccc
    11221 gccagtcctg ttcaccatta ttcagccatg gttcaagggt ataacgagac agaccaaagc
    11281 ctgccaccgt attgacatct tcatgcagca ggcctgcttt gagcagttca cgcaccagaa
    11341 ccggtacgcc acctgccgcc tggaagtggt taatatcggc cggaccgttc gggtagagac
    11401 gtgccatcag cggtacaaca tcagaaaggt cagagaagtc atcccagtta atctgaatac
    11461 cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt agtggaacca ccggtcgcca
    11521 gcagtgcaac gataccgttc accaccactt tctcatcgat catcttaccg atcggcatcc
    11581 attcattacc attaccggtc atgcgtgtaa cctgacgcgc agcggcagcg gtcaaagcat
    11641 cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg caactgcatc cccataaatt
    11701 ccaccaccat ctggttggtg ttggcagtac cgtagaaagt acatgttccc ggcgcatggt
    11761 aagacgcggc ttctgactcc agtaaggcca tgcggtccac tttaccttcg gcataaagct
    11821 ggcgaatacg cactttttct ttatttggca aaccgcttgc catcggtcca gacggcacaa
    11881 acaccgcagg caaatgacca agcgacaggg ctgccatcgt cagacccggg acaatcttgt
    11941 cgcacacacc gaggaacaga gcaccatcaa acatgttatg ggacagcccc accgccgcag
    12001 acatcgctat cacttcgcgg cttagcagcg acaattccat tccatcctgc ccctgggtga
    12061 caccatcaca catcgccgga acaccgcccg caacctgacc aaccgcattc gcttcatgca
    12121 gggctttacg aatgatttct ggatagtgtt cataaggctg gtgcgcggag agcatgtcgt
    12181 tataggaggt gatgatggcg atattgttac gcaacatgct tttcaaagag gctttgtctt
    12241 ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca tgccaactgc gaacgatgaa
    12301 cggtcgacgt tttcgcttgt tctatccggg cgagataagc agagcgagtc tcgcgcgaac
    12361 gttcaatgat tcgatttgtt acgcataaca attgtggatt cataaaggct cctgaaattg
    12421 agttgtcaga gcaggatgat tcacaacgcg tttcattcag aggatttatg agtgaaacgc
    12481 ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt gtaataaaaa aagcctcgtg
    12541 ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct acaatctgtg caagatcatg
    12601 ttaccggtaa aataaccata aaggataagc gcagatatta ctcaaactca ttccaggaac
    12661 gaccatcacg ggtaatcatc gccaccgagg caacgggtcc ccaggttccg gcctgatacg
    12721 gtttcggcgc atcattgtcc atcgcccacg cctcagtaat ggagtctacc catttccagg
    12781 cttcttccac ttcatcacga cgtacaaaca gtgcctgaat accacgcatg gtttccagca
    12841 gcagacgttc ataggcatcc gccagatgcg tctgattaaa ggtttctgaa tagctcagat
    12901 ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc aggaacttta ttcagtacct
    12961 ggatatccac gccttcatca ggttgcagac ggatagtcag tttattctgc ggcagatcct
    13021 gccacgactc tttaaacaga ttcagttcag gtgttttgaa atagaccacg acttcagaac
    13081 atttggtcgg cagacgttta ccagtacgca ggtagaatgg cacaccggcc cagcgccagt
    13141 tatcaatgtc gacgcggatc gccacgaagg tttctgtatt gctgctcttg ttcgcgccct
    13201 cttcttccag atatcccggc acttttttgc cctgggcgaa gcccgcagta tattgcccgc
    13261 gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg cagggacttc agtactttca
    13321 ctttttcatc gcggatgctg tctgcgctca ggtcagatgg cggagacatc gcaatcatgc
    13381 agagaatttg tagcaggtgg ttctgaatca tgtcacgcat ctgaccggct ttatcaaaat
    13441 agccccagcg cccttcgatc cccacttctt ctgccacggt aatctcaaca tgatcaatgg
    13501 tgcgattgtc ccagttattc acaaacaggg agttagcaaa acgcagcgcc aacaggttca
    13561 gcaccgtttc tttaccaaga tagtggtcga tacggtaaac ctggcactcc tcgaagtatt
    13621 cgccaacctg atcattgatt tcctgcgagg tcgccagcga cgtccccagc ggtttctcca
    13681 tgactacgcg tgccggttta gcattcagtt ttgcctcgcc aagccctttg caaattgcgc
    13741 caaaagtgct gggcggcatg gcaaagtagt taatggtgat acgatttttt tgatccagca
    13801 tcgcgccgag acggttgaat gcagcagtgt cattgacatc gagattacaa aaatccagac
    13861 gtgcactcag ggtgtcccat aaaccttcat caatggtttc tttcatgaaa gtttcgagcg
    13921 cctcgcggac aactttggta tatgccgctt tatcccagtc agcacgccct acgccgataa
    13981 tccgggtgtc cgggttgagc tgaccggctt tttccagttg atacagggaa ggcagcaatt
    14041 tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag gtcacaggcc tgggctgttt
    14101 gcgttaccgc catgtcattc tccttaagtt aactaacccg gtacttaagc cagggtatac
    14161 ttgtaatttt cttacggtgc actgtactgc ttttacgagc ttgcgaaaac tgtaaacgct
    14221 tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg gcaaaactga taaaaaaatc
    14281 atcgttttcc attcggtaaa aatctgacac tgatcatgtt atgaaaaaaa ataacaactt
    14341 ttttatctgc ttttgtcatt aacacggcac acaggcgtaa tatctgacaa aactgcattt
    14401 cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc tctttacatc atgaatatgc
    14461 tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc agagcgcaaa gttgccgagg
    14521 tcattctggc ttcgcccgat aacgcgatcc attcgagtat tgctgctatg gcactggaag
    14581 ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag catggacacg cgcggttttc
    14641 ctgattttaa acttcatctg gcacagagtc tggcgaatgg cactccctat gttaatcgca
    14701 atgtcaatga agatgacagc gttgaatcat acacagggaa aatatttgag tccgcaatgg
    14761 caacgcttga tcatgtccgt cattcactgg ataaatctgc catcaaccgc accgtcgact
    14821 tgctcactca ggcaaaaaaa atcgcctttt tcggattagg ctcttcagcc gccgttgccc
    14881 acgatgcgat gaataagttc tttcgtttta atgttccggt ggtgtactcc gatgatatcg
    14941 tgctgcaacg catgagttgt atgaattgta gcgacggaga cgtggtggtg ctgatttctc
    15001 acactggaag aacaaaaaat ctggtcgagc tggcgcagct ggctcgcgaa aacgacgcaa
    15061 tggtgattgc cctcacctct gcgggtaccc cgctcgcccg ggaagcaacg ctggcaatta
    15121 ccctcgacgt accggaagat actgacattt atatgcccat ggtttctcga cttgcacagc
    15181 tgaccgtgat agatgtgctg gcgacaggat ttactttgcg acgcggtgca aaattcagag
    15241 ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg ttttgataag cagttactta
    15301 atttaagtga cgatcgctaa aaacgactgt cactgtccta atcttatacg acatccgaat
    15361 gagattaatt tatcgccatc gcggcgttat ttcattcgga tttcatgttc aagcaacacc
    15421 tggttgtttc agtcaacgga gtattacatg tccagaaggc ttcgcagaac aaaaatcgtt
    15481 accacgttag gcccagcaac agatcgcgat aataatcttg aaaaagttat cgcggcgggt
    15541 gccaacgttg tacgtatgaa cttttctcac ggctcgcctg aagatcacaa aatgcgcgcg
    15601 gataaagttc gtgagattgc cgcaaaactg gggcgtcatg tggctattct gggtgacctc
    15661 caggggccca aaatccgtgt atccaccttt aaagaaggca aagttttcct caatattggg
    15721 gataaattcc tgctcgacgc caacctgggt aaaggtgaag gcgacaaaga aaaagtcggt
    15781 atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg acatcctgct gctggacgat
    15841 ggtcgcgtcc agttaaaagt actggaagtt cagggcatga aagtgttcac cgaagtcacc
    15901 gtcggtggtc ccctctccaa caataaaggt atcaacaaac ttggcggcgg tttgtcggct
    15961 gaagcgctga ccgaaaaaga caaagcagac attaagactg cggcgttgat tggcgtagat
    16021 tacctggctg tctccttccc acgctgtggc gaagatctga actatgcccg tcgcctggca
    16081 cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg aacgtgcgga agccgtttgc
    16141 agccaggatg caatggatga catcatcctc gcctctgacg tggtaatggt tgcacgtggc
    16201 gacctcggtg tggaaattgg cgacccggaa ctggtcggca ttcagaaagc gttgatccgt
    16261 cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc agatgatgga gtcaatgatt
    16321 actaacccga tgccgacgcg tgcagaagtc atggacgtag caaacgccgt tctggatggt
    16381 actgacgctg tgatgctgtc tgcagaaact gccgcagggc agtatccgtc agaaactgtt
    16441 gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc cgagcatcaa cgtttctaaa
    16501 caccgtctgg acgttcagtt cgacaatgtg gaagaagcta ttgccatgtc agcaatgtac
    16561 gcagctaacc acctgaaagg cgttacggcg attattacca tgaccgaatc gggtcgtacc
    16621 gcgctgatga cctcccgtat cagctctggt ctaccaattt tcgccatgtc gcgccatgaa
    16681 cgtacgctga acctgactgc tctctatcgt ggcgttacgc cggtgcactt tgatagcgct
    16741 aatgacggcg tagcagctgc cagcgaagcg gttaatctgc tgcgcgataa aggttacttg
    16801 atgtctggtg acctggtgat tgtcacccag ggcgacgtga tgagtaccgt gggttctact
    16861 aataccacgc gtattttaac ggtagagtaa tctcacctgc cccggccgct gatggccggg
    16921 gtatttagca acacataaat taggactact tattcaatcc aatcataatc acagccccct
    16981 gtaaaatgaa tacagggata gcctgaatgg agatctaaat agcttgcaag acgtcgcata
    17041 cccgcttcct ctgcaacact atgtccaata actgccatcg gtaagccact atctttggag
    17101 tattgtgtct gcacccatgt tttaattgaa tcgttaatcg taatataata atcagcctga
    17161 tattccatat attgtattgg atcacaaaaa cagcctgccc caatgccaac agttctgact
    17221 actctggctt tatctccata aaattgaatg ccaggaagat tatatatttt caaatttttg
    17281 gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta tagcatatac acagtacatc
    17341 tcatcagaag caacaatatt tgtaacatcc agttctaact gctgagccaa agccttagac
    17401 attccgaacg taggctctcg atccatgaca tcatggcaac gaatgattac caaattattt
    17461 ttatgcaaaa actccttttt ttgctcaatt atttttaaat aactgagttc accgcgagta
    17521 taatccatta tttttctggc ccatgaaaac tcaagatcgt caccctccag atcagcataa
    17581 gaataaaatg taggctcatg tgtaataatg atattgacac cttcaaaaac agcctttctt
    17641 aaaacatcca ttgaggcaat ccagcaggtc ccaattttat taataagtgt cgcctcagca
    17701 ccaaaaataa ttttatcaac agttggttgc gaaacagcaa ccattgaatt taaaaaacaa
    17761 ttaagatccg cggctgtttt aatatgatgc attcaacatc cttcacaggt ttcatcagca
    17821 ataaaataat ttcaacattt ctgggaaacg ttttgcccat gcagactcag agtgggtatc
    17881 acctccccat a
//
LOCUS       DAJTYZ010000085        17847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000085 DAJTYZ010000000
VERSION     DAJTYZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17847
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.085"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..304)
                     /locus_tag="OO596_002729"
     CDS             complement(<1..304)
                     /locus_tag="OO596_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6718550.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(514..1032)
                     /locus_tag="OO596_002730"
     CDS             complement(514..1032)
                     /locus_tag="OO596_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCR6718551.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1729..2229
                     /gene="tssB"
                     /locus_tag="OO596_002731"
     CDS             1729..2229
                     /gene="tssB"
                     /locus_tag="OO596_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCR6718552.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2264..2488
                     /locus_tag="OO596_002732"
     CDS             2264..2488
                     /locus_tag="OO596_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718553.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2539..4014
                     /gene="tssC"
                     /locus_tag="OO596_002733"
                     /pseudo
     CDS             2539..4014
                     /gene="tssC"
                     /locus_tag="OO596_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            4021..4434
                     /gene="tssE"
                     /locus_tag="OO596_002734"
     CDS             4021..4434
                     /gene="tssE"
                     /locus_tag="OO596_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6718554.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4438..6288
                     /gene="tssF"
                     /locus_tag="OO596_002735"
     CDS             4438..6288
                     /gene="tssF"
                     /locus_tag="OO596_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6718555.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6252..7334
                     /gene="tssG"
                     /locus_tag="OO596_002736"
     CDS             6252..7334
                     /gene="tssG"
                     /locus_tag="OO596_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCR6718556.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7359..8639
                     /gene="tagH"
                     /locus_tag="OO596_002737"
     CDS             7359..8639
                     /gene="tagH"
                     /locus_tag="OO596_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCR6718557.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8636..9160
                     /gene="tssJ"
                     /locus_tag="OO596_002738"
     CDS             8636..9160
                     /gene="tssJ"
                     /locus_tag="OO596_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCR6718558.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9163..10492
                     /gene="tssK"
                     /locus_tag="OO596_002739"
                     /pseudo
     CDS             9163..10492
                     /gene="tssK"
                     /locus_tag="OO596_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10497..11258
                     /gene="icmH"
                     /locus_tag="OO596_002740"
     CDS             10497..11258
                     /gene="icmH"
                     /locus_tag="OO596_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCR6718559.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11267..14044
                     /gene="tssH"
                     /locus_tag="OO596_002741"
                     /pseudo
     CDS             11267..14044
                     /gene="tssH"
                     /locus_tag="OO596_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            14041..14784
                     /gene="tagO"
                     /locus_tag="OO596_002742"
     CDS             14041..14784
                     /gene="tagO"
                     /locus_tag="OO596_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCR6718560.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14789..16201
                     /gene="tssA"
                     /locus_tag="OO596_002743"
     CDS             14789..16201
                     /gene="tssA"
                     /locus_tag="OO596_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCR6718561.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16220..>17847
                     /gene="tssM"
                     /locus_tag="OO596_002744"
     CDS             16220..>17847
                     /gene="tssM"
                     /locus_tag="OO596_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCR6718562.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPYV"
BASE COUNT         4151 a         4894 c         4876 g         3926 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 tcgtcaggta ggccattttg tccagtacct gcggaaattc aagggagaga aactgctggc
      181 tgaccagaga gagctcgagg gaaaagagcg aagagagtga ctggttcaga tgaaaggaga
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag cgtgaaacgt aatccggttg
      301 acatagcaac ctccggggtt aaggaaaaac agacagttcg ctgttaaagc ggctgtgaca
      361 gtacagtgtt acgcttgcgt tcaggcacgc taccgccgaa gtttccggca tccgccatct
      421 cccgtgaacg taacccctta ctgacaacct ttctttctac ggcgtaatac aaaaacagca
      481 tccggcctga tagccggatg ctgttggctt aacttatgct tccagtggtg cgcgccagtc
      541 gtcggcaccg gaggtgccag atttgacgtg ttcccattca attttgcggt aggccagtga
      601 aacctcgagc aactgggtga actcgcgttt cgccgggtcc tggcaatgcg gcatatgcag
      661 tttcatatcg acaatcgtcg aatcagtcag gcgggtagtg aaataatgct cctgtttgcc
      721 ctccacagag gtacgccacc agtgaagttc agtggttggc agcatttcgc cggaggccag
      781 tgcgttgtac atcagcggaa ccgctttgtt cagcgccacg gtaaaaatga aaggcttgtg
      841 agcacgctga cccgcaggct gaccggactg aggagtggtc gggacagtaa cgttatgcag
      901 gaattcctgc accagcatct cgtcttcgtg cccctgcaca tagatgttgc cgacagaatc
      961 agcggtaaat gcacctgcag taatatttcc ctgggtctga ccagtgatgg agatataaca
     1021 cggtgttggc atttgtaaac tccttgttaa cgtggttaat tactacttac aatgacctgc
     1081 cgttaatccc agacaggcag atgcagctca cttcacggaa ccgcatcatt tttcagtatt
     1141 ttttacatac aaaccgagct agaatttacg gttgtcgtaa aaactatttt taaaatcatt
     1201 aaatctctta ttcagcaata acttaataat atatatctgg ataaccatac ccaactgttc
     1261 aaagtccgta ttgcctccat aaaatggcca aagctgaatc atgaataaat attgtattta
     1321 tcctttctga attatcattt tgataatttt tgagatattc cttaaaaata cccacttaat
     1381 caatcacaaa acaaatatat aaacaagcaa tcattagatt agcttatttt taatgaaatt
     1441 attatatact tcaataagtt acacaaaaac tctatattta aattattgcc ataatacacc
     1501 tgaagcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
     1561 gatcgataaa aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga
     1621 gtggtgattt tattttcacc gccattaggg cggcattgcc gatatttttt tgcggtacaa
     1681 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
     1741 tttgaaggca gcgtcgcgcc ccgcgagcgt attaatatat cgtatgtccc aaaaacagat
     1801 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
     1861 acgcaggaaa catccccact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
     1921 ggtgcagtga tggctgaagc agccattggc ctcaatttta ctgtccccgc cacgctgaag
     1981 ggaagcacaa cagacgatga actgaatgtg gcgctgaata tcaaatcact ggatgatttt
     2041 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
     2101 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
     2161 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg tctggtgagc
     2221 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
     2281 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
     2341 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
     2401 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
     2461 gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc ggtttcagaa acacagctaa
     2521 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     2581 cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     2641 ggttcggaag gttatgacgt tgccctccag ggtgtgacag cctttattgc cagcatcctg
     2701 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     2761 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     2821 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     2881 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     2941 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     3001 ggcggtaaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     3061 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     3121 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     3181 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     3241 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     3301 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     3361 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     3421 taccgctgat gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     3481 atgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     3541 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     3601 gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa gcactttccg
     3661 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     3721 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     3781 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     3841 aacccgcctg ctgacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     3901 gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc tcattttaaa
     3961 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     4021 atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     4081 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     4141 cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     4201 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     4261 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     4321 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     4381 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     4441 gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct tagcaagctg
     4501 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc agatccggat
     4561 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     4621 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     4681 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     4741 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     4801 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     4861 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     4921 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     4981 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     5041 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     5101 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgct gccctggccg
     5161 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     5221 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     5281 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     5341 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     5401 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     5461 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     5521 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     5581 catcagatag aatacagccg gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca
     5641 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     5701 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     5761 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg aaaaaatgcg
     5821 gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     5881 ggcagcctgc actggcccct actctccgcc atgaacctga attatctgtc attactggat
     5941 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca tcatccgcag
     6001 acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     6061 gatcgcctgt ttacgggtat tcccgtccgg gggctggctt ccacgctgta tctgcacccg
     6121 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     6181 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     6241 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     6301 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     6361 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     6421 cagaattatc cctgcgcgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     6481 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     6541 ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     6601 tcgctcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     6661 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     6721 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     6781 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     6841 ccgggatcct ggcaaatccg ggacgatccc cggaaattat ctgtggcctg gtgtcccact
     6901 gctttgattt gtcagaagtg acactgcaaa actggcaacg tcgcaaggtg gatattgaac
     6961 cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     7021 gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc ggaaaattcc
     7081 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     7141 acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     7201 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     7261 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     7321 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     7381 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc
     7441 tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg gtacaggatc
     7501 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     7561 tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     7621 taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     7681 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     7741 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca cactatccgg
     7801 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct gccgatccac
     7861 tccaggctct tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     7921 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     7981 ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac gtctctgcgg
     8041 cagaaatggc acaacgccac gttgcggtca cccccttact gcgcggtctt ggcggctcgc
     8101 ttaccatgag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     8161 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     8221 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     8281 acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     8341 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcgatt gtggagtcgc
     8401 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     8461 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     8521 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     8581 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     8641 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     8701 gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     8761 accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca atgcggaagg
     8821 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgctggc
     8881 agccgactat gaccagatag ccagcacgcc gctaccggat gtgctgggta aaaactatat
     8941 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cggtaaagct
     9001 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     9061 atggaaacaa atcgagacgg tggaaggaat cggccaccat taccgcctgc tggtgcatgt
     9121 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     9181 aaggtgatgt ggcaggaagg gatgctgatg cgttcacacc atttccagca gcagcagcgt
     9241 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     9301 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     9361 tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     9421 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct cgctctgccg
     9481 gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaatcgggcg ttaccggctg
     9541 aattatgccg atgtccggga tttgcattca gaagaaggtg acgcgcgaac attgacgctg
     9601 ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta tattacgctc
     9661 ccgctttgcc gtatcagcga tcgccgccga tggctccctg acgttggatg acgattttat
     9721 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     9781 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     9841 cggcatcgcg gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg
     9901 gtttacccat cacgcccgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc
     9961 cgggttgctg ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga
    10021 tgtgtatgat catcatgacc tgaccaaaat atttaaaacg ctgttaccgg aagtcaaacg
    10081 ggcgctgcat accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg
    10141 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
    10201 cgtggcggca aacatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
    10261 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atccccggta ttccattgcg
    10321 tgcccttatg gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact
    10381 cgacaagagt ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
    10441 ttccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
    10501 atgaggggtc actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcaact
    10561 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac
    10621 tcggtatggt aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg
    10681 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
    10741 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
    10801 gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
    10861 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
    10921 aacgttacca ggatctgctg gaatttctct ggatctgttt ttctctggga tttcgcgggc
    10981 gctacaaagt ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
    11041 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
    11101 cccagggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
    11161 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
    11221 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
    11281 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
    11341 ctctgaatgt atgagtcagc aggcagcgga aattaccgtc agccatgtgc tcattcagat
    11401 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    11461 agagttgcgc caggcgctga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    11521 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctagctgc tggcgtcggc
    11581 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc
    11641 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    11701 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    11761 cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc gctatgccaa
    11821 aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga
    11881 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccgg tggtggtggg
    11941 cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca tcgtggcagg
    12001 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacttgg gcgcattgca
    12061 ggccggggcg tcggtgaagg gtgaattcga aaaacgtttc aaagggctga tggcggaggt
    12121 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc tgattggcgc
    12181 gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg
    12241 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    12301 tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    12361 caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga
    12421 tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt
    12481 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    12541 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    12601 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    12661 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcctggca
    12721 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    12781 agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc agccagagtc
    12841 agagtcagag tcagagtcag agcaggataa taccggtgcc gaaccggctg atgaggccga
    12901 cagagaacaa ccggaagaga ccgctgaaac agtttccccg gtacagcggc tggcccagct
    12961 cactgccgaa ctggacgccc tgcataacga ccggttgctg gtctccccgc acgtcgataa
    13021 aaaacagatt gcggcggtga ttgccgaatg gaccggcgtg ccgctcaacc gcctgtcgca
    13081 gaatgaaatg tcggtcatca ccgacctgac gaaatggctg ggtgacacca tcaaaggcca
    13141 ggacctggcg attgccagcc tgcataagca tctactgacc gcacgcgccg acctgcgtcg
    13201 tccgggacgc ccgctcggtg cgttcctgct ggctggcccc agcggtgtgg gtaaaaccga
    13261 aaccgtcctg caactggcag agctgctcta cggcggtcgc cagtacctga ccaccatcaa
    13321 tatgtccgaa ttccaggaga aacacaccgt ctcgcggctg attggttcgc ctccgggcta
    13381 cgttggctac ggtgaaggcg gcgtactgac cgaagcgatt cgccagaaac cctactcggt
    13441 agtactgctc gatgaagtgg aaaaagcgca cccggatgtg ctcaacctgt tctaccaggc
    13501 gttcgataag ggcgaaatgg cagacggtga aggccgcctg attgactgca aaaatatcgt
    13561 cttcttcctg acgtccaacc tcggctacca ggtaatagtc gagcatgccg atgacccgga
    13621 aaccatgcag gaagtacagt atccggtgct ggccgacttc ttcaaacctg ccctgctggc
    13681 gcgtatggaa gtggtgccgt atctgccgct gtcgaaagag acgctcgcca ccattatcgc
    13741 cggaaaactg gcccgtctgg ataacgtgct gcgcagtcgc tttggtgtag aagtggtcat
    13801 tgaaccggaa gtgacggacg aaatcatgag ccgcgtcacc cgcgcggaaa acggcgcgag
    13861 gatgctggaa tcggtcatcg atggcgacat gctaccgccg ctctcgctgc tgctgttgca
    13921 gaaaatggcg gctaacacgg cgattgcccg gattcggttg tcggcagtgg acggcgcatt
    13981 tacggcagac gtggaagatg ctcagaacga cgagtccgtc acaaaggatg aaacggtttt
    14041 atgagcaggt tgcgcgtttt ccggcagaca ggatggttta tcgcgggcct gatgacgggc
    14101 ttacccgcca ctgccgcgcc cgcagaagca tcgtcaatgg ccggtgtggc ggtcgccgtc
    14161 gccaccacga ccccgccgga tgcgacagcc acgttacagg ccatgcagtc ctgtcggcgg
    14221 gagtccgcgg cgctggagcg gcttgactgc tatgaccgcc tgctggcacc gctgtccccg
    14281 tcagggtttg acggggcgct ggtcaaagcc ggttttgtgg gcgaggcctg gacccgcgcc
    14341 acagagcagg aaaaacgccg cgagggcaac accacagaac tgctggtgac gcaggtgccg
    14401 ggggagcgcc cgacggtggt gataaccacc ccggctatcg gtcatgttcc gccacgcccg
    14461 gtgctgatgt tcagttgcgt ggacaatatc acccggatgc aggtggcgct gatgcaccct
    14521 ctggatgtcc atgatatcgc cgtcactctg aatgccgaca accgggcatt gcgcagccac
    14581 tggtttgtgc gcgagaacgg caccctgctg gagtccagcc gggggttatc cgggattgat
    14641 gaaattaagc aactgttcgg cgcaaaaacg ctgacggtgg ataccggcgc agacaatgcc
    14701 gccggaaagc tgacctttaa cattgacggg ctggcaaggg cgattgctcc cctgcgcgat
    14761 gcctgccact gggcgggaga ataaaaatat ggcaatggac ttacgcgatc cgaatgtctg
    14821 gatatcgcac ctgctggaaa acctgccgga agagaaactg gcatcggcgc tgaaagatga
    14881 caacccgaac tgggagtata tcgacggcga aatcgtcaag ctgggatcgc tggcccacag
    14941 tcaactggat atcccggaac tccagcgccg ggggctggtc attctggctt cggagagtaa
    15001 ggattttcgt ctgctggcgc atctgctaag aaccctgcaa cacgccggtg atcctcatct
    15061 cgcattgcgc ctgctggcgc tgtatgtgga gcattactgg gcggtggctg cgccgcagaa
    15121 tatggcgcat aaaaaacgct ttgccagcca ggtcattaaa cgctttgaaa caggtattga
    15181 agttttttca cagaacgccg ccacagcgca gcgcgatgcg ctgtcagggg aactggcaaa
    15241 actggcgcaa tgctggcagt cacataacgc cccggaactg gcacaggcta ctgatgatct
    15301 ttttgccctg taccagcgtg cgtttaatcg tgcggcaccg gctcctgtcc ccactccggc
    15361 ggcctccggt agttcaccac agaccaccgc cacgtctgaa agcggcgtga cgcaacccag
    15421 tgctccggct ccccaaatcg tcatcgacag tcacgacgac aaagcctggc gcgacacgct
    15481 gttaaaagtg gcggccattt tatgtgaacg ccagccggat tcaccgcagg gctaccgtct
    15541 gcgccgccat gcgctttggc agaacatcac cagtacgccc caggcggaaa gcgatagccg
    15601 tactccactg gctgcggtct ccgccgatat ggtggcagat taccatgctc agctcggtag
    15661 tgccgatatg gcgctgtggc agcaggtgga gaaaagcgta ctgctggctc cttactggct
    15721 ggatggtcac tgcctttctg cacagacggc gctgcgtctg ggttacaaac aggtggcaga
    15781 cgccatccac gatgaggtca tccgcttcct tgagcgtctg ccccagctta ccgggctgct
    15841 gtttaatgac cacacaccgt ttatcagtga gcagacgaaa caatggctgg cagcttcgcc
    15901 cgacgccaaa gttgcaccgg ttgcgcaaat cggtgaggaa tcgaaggcag ccagagcctg
    15961 ttttgctgag cagggtctgg aggcggcgct gcgatatctg gacatgctac ccgaaggcga
    16021 tccccgcgat cagtttcacc gccagtacct tgccgcacag ttgacggaag aggcggggct
    16081 ggtacagctt gcgcagcagc agtaccggat gttgttcagg atgggattgc agatgatggt
    16141 ggcagactgg gagccgtccc tgcttgagca gctagaacaa aaattcacgg cagaacaata
    16201 aaagagaaca ggagtttttg tgttcagatt accaacaccc cgactactca gcggactcaa
    16261 atcagccctg cgaccggcga tgccccggtt taaagtctct gctttctggc tgctgatact
    16321 ggcgtggatt tttctgctgg tgtggatctg gtggaaaggc ccgatgtgga cgctgtatga
    16381 agaacagtgg ctcaaaccac tggcgaaccg ctggctggca acggcggcgt gggggattat
    16441 tgccctggtg tggcttaccg tccgggtgat gaagcgcctg caacagcttg aaaaaatgca
    16501 aaagcaacag cgcgaggaag ccgttgatcc gctcagcgtg gaactgaacg cccagcagcg
    16561 ttatcttgac cgctggctgc tgcgcctgca acgccatctc gacaaccgcc gtttcctgtg
    16621 gcagttgccg tggtatatgg tcatcggccc ggcgggcagt ggcaaaacga cactgctgcg
    16681 cgaggggttt ccgtccgaca ttatttatgc cccggagggc gcacgcggcg cagaacaacg
    16741 cctgtacctc acgccccatg tcggtaaaca ggcggtgatc tttgatatcg acggcacact
    16801 ctgcgctccc gctgatgcgg atatcctgca tcgccgtctg tgggaacatg ccctcggctg
    16861 gctgaaagaa aagcgcgcgc gccagccgct gaacgggatt attctgacac tcgatttacc
    16921 cgatctgctg accgcagaca aacgccgccg cgagcatctg ttacagacgc tgcgcagccg
    16981 cttacaggat atacgccagc atcttcactg ccagttaccg gtttacgtgg tactgacccg
    17041 gcttgattta ttacagggtt ttgccgccct gttccagtca ctcaacagac aggatcgcga
    17101 tgcgattctg ggcgtcacgt tcacccgccg tgcccatgaa aatgatgact ggcgaacaga
    17161 gttgaatgct ttctggcaga catgggtgga tcgaatgaat ctggcgttgc cggatctgat
    17221 ggtcgctcag actcacaccc gcgcgtcttt attcagtttt tcccgccaga tgcagggaag
    17281 ccgtgaaccg ctggtgtcac tgcttgaggg tctgcttgat ggcgaaaata tgaacgtgat
    17341 gctgcgtggt gtctatctca cctcttcgct tcagcgtgga cagatggatg atatattcac
    17401 ccagtctgcc gcccgccagt accggctggg caataaccca ctggcgtcct ggcccctggt
    17461 ggacaccgcg ccttatttca cccgcagcct gttcccgcag gcattactcg cagagcctaa
    17521 tctggcaaca gagagccgcg cctggctgat acgttcccgt cgccgcctga cggttttctc
    17581 tgccacaggc ggcgtggcag cactgctgct catcaccggc tggcatcact attacaacgg
    17641 taactatcag tccggcatca ccgtgcttaa gcaggccaaa gcctttatgg acgtgccgcc
    17701 tccgcagggg gaagatgact ttggcaacct gcaactgccg ctcctgaacc cggtacgcga
    17761 tgccacactg gcctatggcg actggggcga ccgcagccgt ctggccgata tgggactgta
    17821 ccagggacga cgtatcgggc cttatgt
//
LOCUS       DAJTYZ010000086        17293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000086 DAJTYZ010000000
VERSION     DAJTYZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.086"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            92..1132
                     /locus_tag="OO596_002745"
     CDS             92..1132
                     /locus_tag="OO596_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6718563.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="OO596_002746"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="OO596_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6718564.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="OO596_002747"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="OO596_002747"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6718565.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="OO596_002748"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="OO596_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6718566.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="OO596_002749"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="OO596_002749"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6718567.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="OO596_002750"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="OO596_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6718568.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9086)
                     /locus_tag="OO596_002751"
                     /pseudo
     CDS             complement(8439..9086)
                     /locus_tag="OO596_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9217..10635)
                     /gene="araE"
                     /locus_tag="OO596_002752"
     CDS             complement(9217..10635)
                     /gene="araE"
                     /locus_tag="OO596_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6718569.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="OO596_002753"
     CDS             complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="OO596_002753"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6718570.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="OO596_002754"
     CDS             complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="OO596_002754"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6718571.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12899..14080)
                     /locus_tag="OO596_002755"
     CDS             complement(12899..14080)
                     /locus_tag="OO596_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6718572.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14335..15564
                     /locus_tag="OO596_002756"
     CDS             14335..15564
                     /locus_tag="OO596_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6718573.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16024..16656
                     /locus_tag="OO596_002757"
     CDS             16024..16656
                     /locus_tag="OO596_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6718574.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            16990..>17293
                     /locus_tag="OO596_002758"
     CDS             16990..>17293
                     /locus_tag="OO596_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6718575.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4581 a         4511 c         3975 g         4226 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctag gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acatggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggtacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt attccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgcc
     8821 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
     8881 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
     8941 tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag cccaccaagc
     9001 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
     9061 cctcccagca aaccaattgt tttcattcct ctctctcctg atgtgtgtta ccgcaacaag
     9121 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa
     9181 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
     9241 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
     9301 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
     9361 ggcagcacca atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac
     9421 ccagttcgtg gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
     9481 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
     9541 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
     9601 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
     9661 cggtttacgc cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt
     9721 cagccctacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
     9781 gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
     9841 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
     9901 accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
     9961 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    10021 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    10081 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    10141 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    10201 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    10261 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    10321 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag
    10381 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    10441 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    10501 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    10561 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    10621 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    10681 acaactcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat
    10741 gtcgttatgt gatggatatt ccaattttca aattaagttg aattattgag attattatta
    10801 accacctaat tttacagcag ataaaattca taaagttcat taattgataa ttgatatgga
    10861 ttatttcata accatgatat gaattatgat gacctacagg tataaaaaac cctgccatga
    10921 ggcagggtca taaaagtaag aggaatgaat taacgagcca gccaaccgcc atctacggca
    10981 atggtataac cattcacata atctgaagcg ctggaggcaa ggaacactat cggccccatc
    11041 aggtcactcg gcagtcccca acgaccagct ggaatgcggt cgagaatttc cgcgctacgt
    11101 tgttcatctg cccgtagttg ttgagtattg ttggtcgcca tgtaacccgg agctatcgca
    11161 ttaacattaa tgttgtgttt agcccattcg ttcgccatca atcgcgtcac gcccatcacg
    11221 ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac
    11281 gtgatattga taatcttgcc gccattgcgt tgcgcgataa agtgtttcgc cgctgcctga
    11341 gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc tttttcgctg
    11401 aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga
    11461 ccaaactcag ctaccgcgcg atccagcagt gctggaatgc catcaatctt tcgcagatcg
    11521 gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gcttgatggt ttcagtcggt
    11581 tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc
    11641 tggcccagtc cagtatcaca accagtgacg accgcaactt taccttcgag agaaaatgca
    11701 cttaaaatca taacaatacc ttattcgttt atgccgacaa ctagcgcaaa tctttaacgg
    11761 ccactatgcc gacaactagc gcaaatcttt aacggccaca tggtccatat catcaaagac
    11821 ctggttttca ccaaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg
    11881 gatcgaccag ctgggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc
    11941 ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata
    12001 aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt tacctggagc
    12061 cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga caaaatattt
    12121 gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc
    12181 cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt
    12241 tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata
    12301 cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg taccggcacc
    12361 gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt
    12421 accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat
    12481 tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa
    12541 aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt ggatgctctg
    12601 tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga
    12661 aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta
    12721 aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg
    12781 aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc
    12841 atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct
    12901 actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca
    12961 gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac
    13021 cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct
    13081 catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct
    13141 cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat
    13201 acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc
    13261 gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag
    13321 ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg
    13381 aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat
    13441 caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc
    13501 gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca
    13561 actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat
    13621 cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa
    13681 gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc
    13741 caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg
    13801 ctttaagtcc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta ttaggaagac
    13861 cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac
    13921 caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca
    13981 cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga
    14041 taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa
    14101 cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt
    14161 ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg
    14221 caaccactaa cccaccaata gatgggtaga taaggcgtta atctcccata cttaacctgg
    14281 tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca gataatgtct
    14341 aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac agccgttggt
    14401 gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt
    14461 actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc
    14521 ctctcatcaa aaacatcagc atgtgaaggg ataacgggcg cggtaacaca ctactatggc
    14581 aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg
    14641 atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt
    14701 gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa tctcattttt
    14761 ctgatgggac gtcatgccac tattcgggta atgggatttt tggtattccc attgattgcc
    14821 tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacgacc
    14881 caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt
    14941 ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat
    15001 ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc
    15061 ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc gtatattgaa
    15121 gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca
    15181 gcatggttgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg
    15241 ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac actacagcag
    15301 gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt ggtatcgctg
    15361 attaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta tgcgatcagc
    15421 ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta tctgatcccg
    15481 gcgcttaaac cctggcgctc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc
    15541 gtatcggtaa tgttctttag ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt
    15601 gaaccattcc atttttcata atattcattt gttattattc cgccctatca cttctctcgt
    15661 catatcctag aattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga
    15721 ctatttaaga tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg
    15781 cgatatagat attctcatac catgagatag ttatctggac cacgttcgac catgcaagat
    15841 taaatcagtg aaacataatc atatttaata acgagaaatg catttttaaa tgtcttaatc
    15901 agaattatag caaatacaga ttaatcgcaa tttttcccag caaaattaca aaatcaagat
    15961 gtattgacat catcaatcac caatgaaatt atgcaattat atacggatag ggaggttctt
    16021 aacatggggc tttgtagtcg ttataaaagt cttacatgca atagttgctc aatgcattgc
    16081 caaataatgc cagaagagtc accgcgtttg cagtattgcg caaactcgtg tttttgtatg
    16141 tggcccgaag aaagttcata ttttaatcgt ggtgtggtgg aaggtatctt aacaaaaaac
    16201 cacaatgcga gattaagcgg atatattttt gtggattttt cagtaagttt tttacgtcta
    16261 ttccttgaaa aagactggat tgactatctt gcaagtactg acatggggat agttttagtc
    16321 agcgaccgca atatgcaatc gttagctaac tactggcgaa aacacaacac cgcaatatct
    16381 gctgtcatat ataatgatga tggtcttgat gttgccaatg aaaagatcag acaactattt
    16441 attggtcgtt atttatcatt taccagaggt aacacattaa ctcagatgga attcaccata
    16501 atggggtata tggtttctgg ttataatcca tatcagattg ctgaagtgct ggatatggat
    16561 atccgtagca tctacgcgta caagcaacga atcgaaaaga gaatgggtgg taaaataaac
    16621 gaattattta ttcgttcaca ttcggtccaa cattgatact caacgaccag ccagaatcat
    16681 actctgttca tacgggcatc tattgaaatg cccgtatttt ataaataagc agtaacccca
    16741 agagtaaaca acatagtatt taaaaccatc acacgaaaaa ttcagaagca ttacgaaata
    16801 tgaatttaca aacaatagtg gcataaatgt taaccatgtt aatttacgta aagttttacg
    16861 ttgcaacatt aaagcctcat ttcaatcatc atgataaata taaaattaat atatatttat
    16921 gccgtaaata ccaatatact tagcaaacta tgtgatctcc atttcgattg atttagtgtt
    16981 tattgacgta tgtactggat tattaacgat aatatcgagt tctggcctga gcaccgaaaa
    17041 ttaatatcgg tacataacgc cgatcttaac gtcgttctga caacgccagc cagtcgatgt
    17101 ttatcacttc tacttgaagc ttttcctgat gtggttgcac aacaagattt tttcaccaga
    17161 gtctgggaag aagaaggtat gcgtgtgcct actaacacgt tatatcagaa catatccatt
    17221 atcagacgcg gatttcgcgc tgttggtgat actacccact cgctaattgc aaccgtgccg
    17281 agagaggatt caa
//
LOCUS       DAJTYZ010000087        17134 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000087 DAJTYZ010000000
VERSION     DAJTYZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17134
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.087"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            192..2039
                     /gene="ldtD"
                     /locus_tag="OO596_002759"
     CDS             192..2039
                     /gene="ldtD"
                     /locus_tag="OO596_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6718576.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2220..2768
                     /locus_tag="OO596_002760"
     CDS             2220..2768
                     /locus_tag="OO596_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6718577.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2795..3442
                     /gene="gloC"
                     /locus_tag="OO596_002761"
     CDS             2795..3442
                     /gene="gloC"
                     /locus_tag="OO596_002761"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6718578.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO596_002762"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO596_002762"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6718579.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO596_002763"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO596_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6718580.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO596_002764"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO596_002764"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6718581.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8301..9503)
                     /gene="pncB"
                     /locus_tag="OO596_002765"
     CDS             complement(8301..9503)
                     /gene="pncB"
                     /locus_tag="OO596_002765"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6718582.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9769..12381
                     /gene="pepN"
                     /locus_tag="OO596_002766"
     CDS             9769..12381
                     /gene="pepN"
                     /locus_tag="OO596_002766"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6718583.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OO596_002767"
     CDS             complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OO596_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6718584.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13352..14143)
                     /gene="ssuC"
                     /locus_tag="OO596_002768"
     CDS             complement(13352..14143)
                     /gene="ssuC"
                     /locus_tag="OO596_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6718585.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14155..15302)
                     /gene="ssuD"
                     /locus_tag="OO596_002769"
                     /pseudo
     CDS             complement(14155..15302)
                     /gene="ssuD"
                     /locus_tag="OO596_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15299..16258)
                     /gene="ssuA"
                     /locus_tag="OO596_002770"
     CDS             complement(15299..16258)
                     /gene="ssuA"
                     /locus_tag="OO596_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6718586.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16251..16826)
                     /gene="ssuE"
                     /locus_tag="OO596_002771"
     CDS             complement(16251..16826)
                     /gene="ssuE"
                     /locus_tag="OO596_002771"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6718587.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4295 a         4420 c         4349 g         4070 t
ORIGIN      
        1 attacggcgc gcttttcttt aaacttcttt acattaggtt atgtaaaaac gtatcggcgt
       61 ttatatactg aagataagcc tgatgagtaa caggcttgct cgtcatactt tcgtgagtat
      121 tggcgttgta caggcaagtc gtaaaataac agcctggcta ttcagagtat gataaaaaca
      181 gggggcaagg gatgttgctt aatatgatgt gtggtcgtcg gctgtcggca atcagtttgt
      241 gcctggccgt aacattcgct ccactgttca atgcgcaggc cgatgagcct gaagtaatcc
      301 ctggcgacag cccggtggct gtcagtgaac agggcgaggc actgccgcag gcgcaagcca
      361 cggcaataat ggcggggatc cagccattgc ctgaaggtgc ggcagaaaaa gcccgcacgc
      421 aaatcgaatc tcaattaccc gcaggttaca agccggttta tcttaaccag cttcaactgt
      481 tgtatgccgc acgcgatatg caacccatgt gggaaaaccg tgatgctgtt aaagccttcc
      541 agcaacagct ggcagaggtg gcgattgccg ttttccagcc gcagtttaat aaatgggtag
      601 agttactgac cgatcctggt gttaacggga tggcacgcga cgtagtgctc tctgatgcga
      661 tgatgggcta tctccatttc attgcaaata ttccggtcaa aggcactcgc tggctatata
      721 gcagtaaacc ttatgcactt gcaacgccgc cgctctcggt gattaaccaa tggcagctgg
      781 cgctggataa aggtcaattg cctacgtttg ttgcaggact ggcaccgcag catccgcaat
      841 atgcggcgat gcatgaatcg ttactggcct tactcagtga caccaaaccg tggccccaac
      901 tgaccggcaa agcaacgttg cgcccagggc agtggagtaa cgacgtaccg gcgttgcgcg
      961 aaatattgca acgcacaggc atgttggacg gggggccgaa aattactcta cctggcgatg
     1021 acacgccaac tgacgcggta gtcagcccat ccgctgttac tgttgaaaca gcagaaacta
     1081 agccgatgga caagcaaacg acgtctcgta gtaaacctgc gcctgccgtt cgcgccgcct
     1141 acgataatga actggtggaa gccgttaaac gttttcaggc atggcaagga ttgggggcag
     1201 atggtgctat tggcccggca acacgtgact ggttaaacgt aacgcccgcc cagcgtgctg
     1261 gcgtgttggc tctcaacatc cagcgattgc gcttgctgcc aacagagctt tctaccggga
     1321 tcatggttaa cattccggcc tattcgctgg tctactatca gaacggcaat caggtgctgg
     1381 attcgcgagt cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc agtgccctta
     1441 acaacgtagt ggtaaacccg ccgtggaacg tacctccaac tctggcacgc aaagatattc
     1501 tgccaaaagt gcgcaacgat ccgggatatc tcgaaagcca tggctatacg gtgatgcgcg
     1561 gctggaacag cagagaagcg attgacccat ggcaggttga ctggtctaca atcacggcct
     1621 cgaatttacc gttccgcttc cagcaggctc caggcccacg gaactcgctg gggcgctata
     1681 aattcaatat gccgagttca gaggccattt atttgcatga cacgccgaac cacaatctgt
     1741 tcaagcgtga tacacgcgca ttgagctcag gctgtgtacg agtgaataaa gcttccgatc
     1801 tggcgaatat gctgttgcag gatgcaggct ggaatgacaa acgtatttct gatgcgctga
     1861 agcagggtga tacacgttac gtcaatattc ggcagtcgat tccggtgaat ctctactacc
     1921 tgacggcctt tgttggtgca gatggtcgta cccagtatcg tacagatatt tacaattatg
     1981 atctgcctgc gcgatccagc tcgcaaatcg tatcgaaagc ggaacaatta atcaggtaaa
     2041 tgaagtagtt cggggaaatc agttgtcgta ataattagca tgattggggg cgattctctg
     2101 cagcccccgt cactgctggg gttgagtcat cttgacgtct gctttacggg cggttaaggt
     2161 gcctcttgtg cgccagaagt gcatataaac gataacattg acctgtagac ttgattatca
     2221 tggacaaatt cgacgctaat cgccgcaaat tgctggcgct tggtggcgtt gcactcggtg
     2281 ccgccatcct gccgacccct gcgtttgcaa cactctctac cccacgcccg cgcattttga
     2341 cactcaataa tcttcatacc ggagagtcaa tcaaagcgga gtttttcgat ggcagaggct
     2401 atattcagga agaattggca aaacttaacc attttttccg cgattaccgc gcgaacaaaa
     2461 taaagtccat cgacccagga ttattcgacc agttgtatcg cctgcaaggg ttgttaggca
     2521 cgcgcaaacc ggtgcaactc atttccggtt atcgttctat tgataccaac aatgaactac
     2581 gcgcccgcag ccgtggagta gcgaagaaaa gctatcacac taaaggccag gcgatggatt
     2641 tccatattga aggtatcgcg ttaagcaata ttcgcaaagc cgcgttatct atgcgcgcag
     2701 gtggtgtagg atattatcca cgtagtaact ttgtgcatat tgataccggg ccagcacggc
     2761 actggtagta attgcttaac gaaacagggg cagtatgaac tatcgtatta ttccggtcac
     2821 cgcattctcc cagaactgtt cattaatctg gtgtgaacaa acccgtctgg ccgcactggt
     2881 cgatcctggc ggcgatgcgg aaaaaatcaa acaggaagtt gatgacagcg gcctgacact
     2941 gatgcagatc ctgctgacgc atggtcatct ggaccacgtt ggcgcagcgg cggaactggc
     3001 gcaacattac ggcgtgccgg tttttggccc ggaaaaagaa gatgagttct ggctgcaagg
     3061 cttgcctgcg caaagtcgta tgtttggtct ggaagagtgc cagccgctga cgccagatcg
     3121 ttggctgaac gaaggcgata ccatcagcat agggaatgtg actttacagg tgttacattg
     3181 ccctgggcat acgccgggtc atgtcgtgtt ttttgatgat cgggcaaagc tgctgatttc
     3241 tggcgatgtt attttcaaag gcggagtagg gcgcagtgac ttcccgcgtg gcgatcataa
     3301 tcaactgatt tcttcaatca aagataaatt gctgccactg ggggatgacg tgacatttat
     3361 tccgggtcac ggaccattat ccacacttgg ttatgaacgc ctgcataatc ccttcctgca
     3421 agacgaaatg cccgtctggt aaggcacata aaaaagcccg cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 tgcttacagc actgccacaa tcgcttcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaaccgc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcaggc cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcgcctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaacg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggtggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacggtttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcattcagg ctgttaatca gtgcatcgaa
     4381 gtcaagagtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacactc gcttaacgct
     4501 ggtatttttt gccaggaaat cggcagctac gcgtagtgcg ccagtgcccc ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggagctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 tataaaaaaa acaggaccaa agtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gctcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggaactt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcggggtt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgcgcgaaat cggcctcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atgggggggg gatacctatg ttacctggca tctgcaatca
     8221 gacaagcaga attcgcaaat gcagcgaaag atttcggaat taaaggtaaa aaaagggagc
     8281 cgattagctc cctgatgata ttaactggct tttttaatat gcggaaggtc gaacgctttg
     8341 cgcagcgccc gaacaaacgc tttatcatgg cagatagttt tgccagggct gtcagaaagt
     8401 ttcgccaccg gtttaccgtt acactctacc aacttaatca caatattcag gggttttacc
     8461 tgggggatat cgcaggtcag gcgagtccca ataccaaaac ttaattgcac gcgggaagag
     8521 aagtggcggt atagctcaac cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt
     8581 ttactctgtg gatcaattcc cagcttttca taatgtgcaa tggctttttc accccattca
     8641 accgggtcgc cagagtcatg acgcaggccc tgataccgac tagcgaactc gacaccgaaa
     8701 tcacgcagga aagcatccat agtgatgcag tcggttaatg caatgccaag ttggtcggga
     8761 tactcttcca gccaggcagc aagtgcagct cgctggctgt tggctagatc cgggctgatt
     8821 tgctgatgtg cctggaacca ttcgtgtgcc tgtgttccca tcggcgtgag ggaaagccga
     8881 cgcgccagat cgtagttgct ggtgcccacg aaccaggatt cctgttgcag acgcttaacg
     8941 atggtttctt gtacttcgcg agaaaaacgg cgacgggtgc caaaatccat cagatggaag
     9001 cgcgacatat caagaccggc ggttaacgcc gagaagtcga ctaatttgct ttccagcgtg
     9061 tcgagggctt gcgcaacgtc ggcctgcggt gagcgatagc gatgtaccat ttcactgata
     9121 accgccagca aaggaacttc ccagaggatg acttcacgcc acggaccgct taagcgaata
     9181 tccagcttgc cattatcgtt ggacacggtg acttgttccg ggttaaagcg gaactcgcgt
     9241 aaccagttaa gatagtcggc cttaaagaaa ggcagggcag agagccactg atattcatca
     9301 tcctgcaggc gcaggtgctg catcgcctga atctgttcac gaatagcatc ggcataaata
     9361 cccagcagat cgtcacctcg gcaacgaaac tccgccgcga catgcacatc gtaatagtga
     9421 tgaaacacgg cttgctgcat atgcaactta taagcatctg tatccagcaa cgagtgcaga
     9481 acaggagaag cgaattgtgt cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt
     9541 acaataaaca tcttcaggaa ctgctggagt ataccttgtt tcgcaattta ttgaaccccg
     9601 atcacaccat atgccacctt tctggtcgat ggcattacgc ggtgtatgtt ataaaaatgt
     9661 agcaataaag gcgtttgtac ctgaaaagat gaagattctg catagcgcga tttacgcaac
     9721 aggaatagac tgaacaccag actctataaa agatgctaaa ggttatttat gactcaacag
     9781 ccacaagcca aataccgtca cgattatcgt gcgccagatt accagattac tgatattgac
     9841 ttgacctttg acctcgacgc gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt
     9901 catggtgcat cagatgctcc acttcgtctc aacggcgaag acctcaaact ggtttctgtt
     9961 catattaatg atgagccgtg gaccgcctgg aaagaagaag agggcgcact ggtcatcagt
    10021 aatttgccgg agcattttac gcttaagatc gttaatgaaa taagcccggc ggcgaatacc
    10081 gcgctggaag ggctttatca gtcaggcgat gcgctttgca cccagtgtga agccgaaggt
    10141 ttccgccata ttacgtatta tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa
    10201 attattgccg ataaaaccaa atatcccttc ctgctttcca acggtaaccg cgttgcgcaa
    10261 ggtgaactgg aaaacggacg ccattgggta cagtggcagg acccgttccc gaaaccgtgc
    10321 tacctgtttg cgctggtggc aggcgacttt gatgtactgc gcgacacctt taccacgcgt
    10381 tctggtcgcg aagtggcgct ggagctgtac gtcgatcgcg gcaaccttga tcgcgcgccg
    10441 tgggcgatga cctcgctgaa aaactcaatg aaatgggatg aagaacgctt cggcctggag
    10501 tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg cgcaatggag
    10561 aataagggtc tgaatatctt taactccaaa tatgtgctgg cccgcaccga caccgccacc
    10621 gacaaagatt acctcgatat tgaacgcgtt atcggccatg aatatttcca taactggacc
    10681 ggtaaccgag tgacctgccg cgactggttc cagctcagcc tgaaagaagg tttaaccgtc
    10741 ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cagttaaccg catcaataat
    10801 gtgcgcacca tgcgcggatt gcagtttgcg gaagacgcca gcccgatggc gcacccaatc
    10861 cgcccggaca tggtcattga gatgaacaac ttctacaccc tgaccgttta cgagaagggc
    10921 gcggaagtga tccgtatgat ccacaccctg ctgggcgaag aaaacttcca gaaagggatg
    10981 caactctatt tcgagcgtca tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg
    11041 atggaagatg cgtcgaatgt tgatctctcc catttccgcc gttggtacag ccagtccggt
    11101 acgccgattg tgaccgtcaa agacgactac aatccggaaa ctgagcagta caccctgacc
    11161 atcagccagc gcacgccagc cacgccggat caggcagaaa aacagccgct gcatattccg
    11221 tttgccatcg aattgtatga caacgaaggc aaagtgatcc cgttgcagaa aggcggtcat
    11281 ccggtgaatt ccgtgctgaa cgtcacccag gcggaacaga cctttgtttt tgataatgtc
    11341 tacttccagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa actggaatat
    11401 aagtggagtg atcagcaact gaccttcctg atgcgtcatg cgcgtaatga tttctcccgt
    11461 tgggatgcgg cgcaaagttt gctggcaacc tacatcaagc tgaacgtcgc ccgtcatcag
    11521 caagggcagc cgctgtctct gccggtacat gtggctgacg ctttccgcgc ggtgctgctc
    11581 gatgagaaga ttgatccggc gctggcggca gaaatcctga cgctgccttc tgtcaatgaa
    11641 atggctgaac tgttcgatat catcgacccg attgctattg ccgaagtacg cgaagcactc
    11701 actcgtactc tggcgactga actggcggat gagctgctgg ctatttacaa cgcgaattac
    11761 cagagcgagt accgtgttga gcatgaagat attgcaaaac gcactctgcg aaatgcctgc
    11821 ctgcgcttcc ttgcttttgg tgaaacgcat ctggctgacg tgctggtgag caagcaatac
    11881 catgaagcaa acaatatgac cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag
    11941 ctgccttgcc gtgacgcgct gatgcaggag tacgacgata agtggcatca ggacggtctg
    12001 gtgatggata aatggtttat cctgcaagcc accagcccgg cggcgaatgt gctggagacg
    12061 gtgcgtggtc tgttgcagca tcgctcattt accatgagca acccgaaccg tattcgttcg
    12121 ttgattggtg cgtttgcggg cagcaacccg gcagcgttcc atgccgaaga tggcagcggt
    12181 taccagttcc tggaggaaat gcttaccgac ctcaacagcc gtaacccgca ggtagcttca
    12241 cgtctgattg aaccgctgat tcgcctgaaa cgttatgatg ccaaacgtca ggagaaaatg
    12301 cgcgcggcgc tggaacagtt gaaagggctg gaaaatctct ctggcgatct gtacgagaag
    12361 atcaccaaag cactggcttg atacttttga gccaaaatgc ctgatgcgac gcttgacgcg
    12421 tcttatcagg cctacaactc cgttgaaatt gctggttttg taggctggat aaggcgttca
    12481 cgccgcatcc ggcagccgtg cccaactgcc tgatgcgatg cttgacgtgt cttatcaggc
    12541 ctacaagtcc gttgaaaatg ctggttttgt gcgccacatc cggcatatta accatgacgc
    12601 cgaatcagtt gttcagaatg cccacgccgc attacccgtt gcaatacttc cgcttccagc
    12661 tccgctaatc gcaccgaccc taatcgacgc ggacggggga tatccactgt caaatccaga
    12721 ccaatttttc cctcttcaat taacaacacc cggtcagcca ttgctaccgc ttcgctcaca
    12781 tcatgcgtca ccaacagtac ggtaaagccg tgctgctgcc aaagtgacac aatcaaatcc
    12841 tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag caacaataaa
    12901 cccggtcgat gaatcaacgc tcttgccagc gccacacgct gtttctgccc gccagaaagt
    12961 gcggcaggcc attccccggc gcgattctcc agtcctactg cagccaacgc ccgacgcgcg
    13021 gcatcgcgcc actggccttt aaggcctaac ccaacgttat caatcaccga tttccacggc
    13081 agcagacgcg catcctgaaa catcattcgc gtgtcttcct gaatctcagc cagcggtgtg
    13141 gtgcccgcta acacatcgcc tgcggttggc gtttccagac ctgccagcag gcgcagcagg
    13201 gtacttttgc caccaccgct gcggcccacc accgccacaa actgacctgc cggaatatgt
    13261 aaatccagtt ggttcaggac gatattttcc gcgtaatgtt tgcttactgc attgagcaac
    13321 aatggtgtgc cctggttcag acgagcagta ttcataccgt ggcctccttc aaatgataag
    13381 ccgggttcca gcgcaaccag aggcgctcta acagctgcgc gctgacgtca gcaagtttgc
    13441 cgagcagggc gtaaagaata atagcgacta ccaccacgtc cgtttgcaaa aactcccgcg
    13501 cattcatcgc cagataacca atgcctgaat tggcagaaat ggtttcggca acaatcagcg
    13561 tcagccacat caggcccaac gcaaaacgca cgccgaccat aattgagggc agggcaccag
    13621 gcaggatcac atggataaac agcggtatgc cggataatcc atagctacgc gccatttcca
    13681 ccagaccgcg atcgatatta cggatgccat gccaggtgtt gatataaatg gggaatagcg
    13741 tgccgagcgc caccagaaag atttttgcgg attcatcaat gccaaaccac aaaatcacca
    13801 gcgggatcag cgccagatgc ggtacgttgc gcaacatctg aattgaggta tccagcagcc
    13861 gttctcccca gcgtgacagc ccgctaatca gtcccagaat caagcccagc gatccgccaa
    13921 ttgaaaagcc aatcagcgcc cgccaggaac tgatcgccag atgctgccac aattcgccgc
    13981 tggcggagag cgtccagaac gccgtcacca ctccttccgg tgaaggcaaa atacgcgtcg
    14041 acagccagcc aaccgaggag gccagttgcc acaccgccac gatgcccacc ggtaaaaacc
    14101 agggggcaac gcgcaataac cacttcttca ctggcgttgc catcatcgtg ttccttagct
    14161 ttgcgcgact ttacggggga taaaatcatt cgccaccgct tcgccttgcg gattcagcgg
    14221 ctgcggctgg ggaatttccg gaatggcgac atccagatgt gggaacagca actcgccaac
    14281 ccgatacgct tcttccagat gcgggtagcc cgaaagcaca aagctgtcga tgccaagcgc
    14341 ggcgtattcg ttgattcgcg cggcgaccgt aggaccatcg cccaccagcg ccgttccggc
    14401 accgccgcgc actaagccaa cgcccgccca taaattgggg ctgatctcca gattgtcgcg
    14461 cttgccgtta tgtagcgccg ccattcgctg ttgtcctacg gaatccgtcc gggcgaatgc
    14521 ggcctgtgct ttggcgatag tttcatcatc aagatgcgag attaaccgct cggcggcctg
    14581 ccacgcttcg tcgttagttt cacgaacaat cacatgcaga cgaataccga aacgaatttt
    14641 gcgtccatgc gcggcagctt tcgcccgcac ttgttcgatc ttctctttta ccagctctgg
    14701 cggttcgccc caggtgaggt agagatcaac ttgttctgcc gccagctcct gggcgacatc
    14761 tgacgatccg ccaaagtaaa gcggaggata cggctgttga atcggcggga agagcagttt
    14821 tgctccccgc acatgaatat gtttaccgtt gaaatcgacg ctttccccca gcaataagcg
    14881 ccccgccaga cctgggtaaa ttccgccgag gcttcgtagc gctcgctatg atcaaggaac
    14941 acgccgtcgc ctgccaactc ttgtggatcg ctgcctgtga ccagattaaa caacgcacga
    15001 ccatttgaga gacggtcaag cgtggcggcc tggcgagcgg caacggtagg tgaggttacg
    15061 ctgggacgca gggcgacaag aaacttcagc cgctgcgtca ccgggatcat cgacgccgcc
    15121 accagccacg catcttcaca cgatcgcccc gttgggatca gcacaccggt atagcccaga
    15181 cgatccgccg cttgcgcaat ttgttgcaga tagccgtgat caaccgggcg tgaaccttct
    15241 tccgttccaa gataatgccc gtcaccatgg gtcggtaaaa accagaacat attcagactc
    15301 ataattgttt tccttccagg tgagtgggct gccagatgcg ctggcgaata tcaacttttt
    15361 tcgacaccag acggttttca taaaacagat ctgccgtttg ctgctgtaag gcggcaactt
    15421 cagcgctcac cggtttgatg gtggtgggag gacgatgatc cagatacgag gcaatcaccg
    15481 gtgccggtaa gcccatcgtt tttgccagta aagcgatgct ttgctcgcgc tggctgcggg
    15541 ttaacgcatc ggcctcacta aaggttgcca gtacgccctg aataaaagcg ccgttttttt
    15601 ctgcataggg gcgagctgcc agataaaacg atccagtttg attgagatcg gtgccgtctt
    15661 tcagcacccg cacgccgccc tgtaataacg cagcggagta gtagggatcc cagatagccc
    15721 aggcgtcaac gttaccttgc tggaacgcgg cgcgggcatc agctggcgtc agataagtgg
    15781 gctggatgtc agtaaactta agtccggcct gacgcagtgc acgcagtaaa aggttgtgtg
    15841 aactggaacc tttctgaaag gcaactttgt gacctttaag atcggctacg gttttgatcg
    15901 ggctgttttc tgccaccaga atcacttcgg ctttgggctt cggtggctcg acgcccacat
    15961 atatcaaatc agccccggca gcctgggcaa agattggcgg aatatccccg gtactgccga
    16021 gatcaatact gccaacgttt aacgcttcca acatttgcgg acccgcgggg aactccaccc
    16081 aggagatttt tgattgcgga tagcgttttt ccagtaactg gtggcttttt gccaatacca
    16141 taccaatact gcctttctga tagcctatac gtaacgcttc aggcgaggat tctgcagcaa
    16201 ccgcaaaagt agagacgcta agcaatcccg ccagcgccag tttaatgatg ttacgcatgg
    16261 acattacctc gcagagacag aaggtcagga acctgaacat cgcggcggtg caatgcctgc
    16321 cagaaagttt ctagcgcggt atcaagacgg gtttgcagat ttggcgtgaa ctggggtttg
    16381 tgatggtaat caataacttg tgagtcatcg gcaaacacgc cgtgcaggag ctcctgggct
    16441 ttcatcgcac ttaaaactgg cttaagggca taatcgaccg ccagcagatg agccaccgta
    16501 ccgcccgtcg ccagcggtag caccactttg ccttgcaaag cgcgctctgg cagcaggtcg
    16561 agcagggttt tcagcgcgcc ggaataggcg gctttataca caggtgtagc gactatcagt
    16621 ccatcagcct gttgcagctg ttcggtgaag gtcttgagtg ccggactatc gaaacgagcg
    16681 taaagcagat cttccggggc gaagttttgc agattccagt gataaacctc tacatccagg
    16741 ccatttagtt tttcccgcgc atattccagc aaggagctgg agcgagaagg aaagcgagga
    16801 ctacccgcca gggtgatgac acgcatactc tctccttata accaattgtt ctttttttgt
    16861 taacattgat aacaattcgg tcagtctgtc ggagagacaa gaaaatgcca aatataaatt
    16921 ttgtgtatct ttttctgaaa ataaactaaa gaaagggctt tatatatcac gcatatttat
    16981 ttgttggata gtcattagat gttgaatcaa tgaattactc gttcagttaa tatttaaata
    17041 ttcatgaaat ctataaatta aagatttgtc acttattgga tttagtatgc ttgatttaaa
    17101 tggcggtcaa ttattgttat tattaagtct aatg
//
LOCUS       DAJTYZ010000088        16627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000088 DAJTYZ010000000
VERSION     DAJTYZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.088"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..452
                     /locus_tag="OO596_002772"
     CDS             <1..452
                     /locus_tag="OO596_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR6718588.1"
                     /translation="DQILNEYDSSGYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVS
                     TSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKI
                     WMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
     gene            complement(508..3363)
                     /gene="valS"
                     /locus_tag="OO596_002773"
     CDS             complement(508..3363)
                     /gene="valS"
                     /locus_tag="OO596_002773"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6718589.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(3363..3806)
                     /gene="holC"
                     /locus_tag="OO596_002774"
     CDS             complement(3363..3806)
                     /gene="holC"
                     /locus_tag="OO596_002774"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6718590.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3940..5451)
                     /gene="pepA"
                     /locus_tag="OO596_002775"
     CDS             complement(3940..5451)
                     /gene="pepA"
                     /locus_tag="OO596_002775"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6718591.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5718..6818
                     /gene="lptF"
                     /locus_tag="OO596_002776"
     CDS             5718..6818
                     /gene="lptF"
                     /locus_tag="OO596_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6718592.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6818..7900
                     /gene="lptG"
                     /locus_tag="OO596_002777"
     CDS             6818..7900
                     /gene="lptG"
                     /locus_tag="OO596_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6718593.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8019..9521)
                     /locus_tag="OO596_002778"
     CDS             complement(8019..9521)
                     /locus_tag="OO596_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6718594.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(9599..10597)
                     /gene="idnR"
                     /locus_tag="OO596_002779"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(9599..10597)
                     /gene="idnR"
                     /locus_tag="OO596_002779"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCR6718595.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(10664..11983)
                     /gene="idnT"
                     /locus_tag="OO596_002780"
     CDS             complement(10664..11983)
                     /gene="idnT"
                     /locus_tag="OO596_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCR6718596.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(12048..12812)
                     /gene="idnO"
                     /locus_tag="OO596_002781"
     CDS             complement(12048..12812)
                     /gene="idnO"
                     /locus_tag="OO596_002781"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR6718597.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(12836..13867)
                     /gene="idnD"
                     /locus_tag="OO596_002782"
     CDS             complement(12836..13867)
                     /gene="idnD"
                     /locus_tag="OO596_002782"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCR6718598.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            14084..14647
                     /gene="idnK"
                     /locus_tag="OO596_002783"
     CDS             14084..14647
                     /gene="idnK"
                     /locus_tag="OO596_002783"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6718599.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            complement(14651..15670)
                     /gene="ahr"
                     /locus_tag="OO596_002784"
     CDS             complement(14651..15670)
                     /gene="ahr"
                     /locus_tag="OO596_002784"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6718600.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            15866..15950
                     /locus_tag="OO596_002785"
     tRNA            15866..15950
                     /locus_tag="OO596_002785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15900..15902,aa:Leu,seq:caa)
     gene            16136..>16627
                     /locus_tag="OO596_002786"
     CDS             16136..>16627
                     /locus_tag="OO596_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6718601.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARL"
BASE COUNT         3980 a         4333 c         4163 g         4151 t
ORIGIN      
        1 tcgatcaaat attaaatgag tatgatagtt ctgggtatgc aaatgatgat attgagaatt
       61 tacagcaatt tatggaaatg caacgtaaaa tgataatcgc gcagttaatc tattattttg
      121 ggattgctgt tagcaccagt tatttaacgg tgtcgttgcg aaatcatttt atgagcaacc
      181 tgtcactgaa tgatgggcgt attcgttttc gctcaacttt aacataccac ggtatgcttt
      241 atcgcatgtg tgcgttggtg gtgatatccg ggattacggg cggtctggct tatccactgc
      301 tgaaaatatg gatgattgac tggcaggcaa aaaatacgta tttgctgggc gatttggatg
      361 accttccttt aatcaataaa gaagaacaac cagataaagg attcttagcc agtatttcac
      421 ggggaattat gccttcttta ccatttctgt aagagataaa aaaggccgga gcatgctccg
      481 gccttcgttt tcatcactgt gttttgatta cagcgcggcg ataacagcct gctgttcaat
      541 cagtttcgct ttcgcttccg catagccttc cagcttctca cgctctttcg cgatgaccgc
      601 ttccggtgcg cgggcgacaa agccttcatt cgccagcttg ttctcgatac ggctgatttc
      661 gccttcgatc ttcgccactt ctttcgccag acgcgccagc tcatcttctt tgttgatgag
      721 gccagccatc gggatcagca gctctgcgcc gtcgatgatc ttcgtaacgg aaaccggacc
      781 tttgtcatcg gcaggcagca cggtgatgct ttccagacgc gccagggttt gcaggaagcc
      841 acggttttca tttacgcgac gttctgcatc cgcgctgcaa ccacgcagca gcagctccag
      901 cggtttgccc ggcgcgatat tcatttctgc acggatgtta cgtaccgcaa cgatcgcctg
      961 tttcagccat tcggtgtcgg ccagtgcggc ttcatcaacc tgagatgcat cgtactgcgg
     1021 gaacggctgc agcatgatgg tgtcggcagt gataccgcaa agtactttca cacgctgcca
     1081 gatggtttcg gtgatgaacg gaatgatcgg atgcgcgagg cgcagcagac cttccagtac
     1141 ggtcaccagc gtatggcgag taccgcgcag ttcggcttcg gtgccaccgt tcattaccgg
     1201 cttagtcagc tccagatacc agtcacagaa ctggttccag gtgaactcat acagaatgcc
     1261 tgcagcgata tcgaagcgga agctgtccag cgcttcgcgg tacgctttga tggtctggtt
     1321 gaactctgcc agaatccagc ggtccgccag cgacagcgtc atttcaccac cgttgaagcc
     1381 gcaatcctga ccttccgtgt tcatcagcac aaagcggctg gcgttccaca gcttgttaca
     1441 gaagttacgg taaccttcca ggcgcttcat atcccagttg atgtcacgac cggtagacgc
     1501 cagcgccgcc agggtgaagc gcagggcatc ggtgccgtgc ggctcaatac cgttcgggaa
     1561 ctgcttctcg gtgcgcttac ggattttgtc cgccagctgc ggctgcatca tattgccggt
     1621 acgtttctcc agcagttccg gcagcgaaat accgtcaacc atatccagcg ggtcaataac
     1681 gttacccttg gatttggaca tcttctggcc ttcgtcgtca cgaatcaggc cggtcatgta
     1741 aacggtgtgg aacggcacct gcggtttgcc attttcatct ttgatgaagt gcatggtcat
     1801 catgatcatg cgggcaatcc agaagaaaat gatgtcgaag ccggatacca tcacgctggt
     1861 tgggtggaac tggcgcagtg cgtcagtgtt ttccggccag cctagggtag agaaggtcca
     1921 cagcgcagaa gagaaccagg tgtcgagaac gtcttcgtcc tgacgcaggg caacgtcggc
     1981 accgaggtta ttttccttgc gcacttcttc ttcgttgcgg ccaacataaa cgttacctgc
     2041 ttcgtcgtac catgcaggga tacggtgacc ccaccacagc tgacgagaga tacaccagtc
     2101 ctgaatatcg cgcatccagg agaagtacat gttttcgtac tgcttcggta cgaactgaat
     2161 gtcgccgttc tcaaccgctt caaccgccgg tttcgccagt acatcggcac gcacgtacca
     2221 ctggtcggtc agcattggtt cgataactac gccgccacgg tcgccgtaag gaacggtcag
     2281 gtcgtgcggt ttaatttctt ccagcaggcc gagtgcgtca accgcagcaa cgactgcttt
     2341 acgtgcagca aaacgctcga gtttctggaa ctctgccggg atttcgctgg aataaacgtc
     2401 agattcgtta cctttggtat cgaacacctg ggcgctttca cggatatcgc cgtcaaaggt
     2461 caggatgttg atcatcggca gcgcgtgacg tttacccact tcatagtcgt taaagtcgtg
     2521 cgccggagtg attttcacgc agccggtgcc tttttccatg tcggcgtgtt cgtcgccaac
     2581 gatcggaata cgacggttaa ccagcggtag aatgacatat ttgccaatca ggtctttgta
     2641 acgcggatct tccgggttaa cggccacgcc ggtatcgccc aacagggttt ccggacgggt
     2701 agtcgcaacc accagataat ctttaccgtc tgcggttttc gcgccgtcag ccagcggata
     2761 gcggatgtgc cacatcgaac ctttcgattc gcggttttcc acttccaggt cagagatagc
     2821 ggtgcgcagt ttcggatccc agtttaccag gcgtttgcca cggtaaatca ggtcttcttt
     2881 atacagacga acgaaaactt ctttcaccgc attggacagg ccttcgtcca tcgtgaagcg
     2941 ttcacgctcc cagtcgacag agttgccgag acggcgcatc tgacgggtaa tggtgccgcc
     3001 ggactccgct ttccattccc agattttgtc gatgaaagct tcgcggccgt agtcgtgacg
     3061 ggttttacct tcttctgcgg caatcttgcg ctcaacgacc atctgggtag cgatcccggc
     3121 gtggtcagta ccgacctgcc atagggtgtt tttgccctgc atgcgctgat agcggatcat
     3181 ggtatccatg atggtttgct ggaaggcgtg acccatatgc aaactgccgg tgacgttcgg
     3241 cggcgggatc atgatgcaga aactttcctg gctttcatcg ccattaggct taaagtagcc
     3301 ctgcttttcc cagtgctcgt aaagcggctg ttcgatatct tgtgggttat atgtcttttc
     3361 cattatttcc aggttgccgt attcaggttg aaaccagcca cgcggtaggc tttatagcgt
     3421 tcgcgcgcca gttgtttcag agaatcttca taaggaacga agtctaccac ttctgtgaaa
     3481 gcagtggcaa aatctgcaaa gcttgttcgc agactaatca atatatcgcg cgggctgctg
     3541 ctgcgttttt gcggccaggc gatctccacc ggcgcaccgc cgcgcggtcc ttctcccgct
     3601 aaattatgcg ggacaaagct ttctgccgga cgcgcccaca gggcttcatc cagccggtaa
     3661 gcctgctttt catcttcaca ggcgatgagc acgcgcttac cgctgcgcca acgttctgcg
     3721 gcaatttcac acaccagttg ctcaacggcg cttaagccat cgacggtggt gtcattgtcc
     3781 agaaggtaga acgttgcgtt tttcatatat ggggcttctt gtggtggatt taaatattgc
     3841 tgcattcctc accctgaaag atgagcaatg aatttctatt gcgctcgatc ggttccctct
     3901 cccttaggga gagggttagg gtgagggtaa aacgcaaact tactcttcgc cgttaaaccc
     3961 agcgcggttt aacaggaact gtgccagcaa cgctaccgga cgaccggttg cgccttttgc
     4021 tttaccagaa cgccaggcgg ttcctgcaat atccaggtgc gcccagttgt acttacgggt
     4081 aaagcgtgac aggaagcaac ctgcggtaat cgccccacca ggacgaccgc caatgttcgc
     4141 catatcggca aaattggact ccagttgttc ctgatactcg tcacccagcg gtaagcgcca
     4201 tgcgcggtca ccggattgtt cagacgcggc aatcagttca tgggccagcg gattatggtt
     4261 cgccatcaga ccagtaatat gatgacccag cgcgatcacg caggcaccgg tcagcgtcgc
     4321 cacatcaatc accgcttccg gctcaaaacg ttcaacgtaa gttaacacgt cgcacagtac
     4381 caggcggcct tcggcatcgg tgttcagcac ttcaacggtt tgaccggaca tggtggttaa
     4441 cacatcgccc ggacgatagg cacgcccgcc aggcatgttt tcacagcctg ccagcacgcc
     4501 aataacgtta ataggcagtt gtagctctgc gaccatccgc atcacgccgt aaaccgctgc
     4561 cgcaccgcac atatcgtact tcatctcatc catgccttct gaaggcttga tggagatacc
     4621 gccggagtcg aaggttaaac ctttacccac cagcacgatt gggcgtgcat cttccgacgc
     4681 gttgcctttg tactcaatca ccgacatcag cgattcgttt tgcgaaccct gaccgaccgc
     4741 cagataagag tgcatcccca gctctttcat ctgctgttcg ccgataacgc gggtgataac
     4801 attcttgctg tagctgtcag ccagctggcg cgcttgtgaa gcgaggtaag cggcgttaca
     4861 gatattcggc ggcatattgc cgagatcttt tgctgcttta atcccggcgg caatcgccag
     4921 accgtgctgg atcgcgcgct caccgctggt cagttcacgg cgggtcggca cgttgaacac
     4981 cattttacgc agcggacgac gcggttcgct cttgttcgtt ttcagctgat cgaaactgta
     5041 gagcgtctct tttgcagtct cgacagcctg acgcactttc cagtagttgt tacggccttt
     5101 aacgtgcagt tcagtcagaa agcagaccgc ttccattgag ccagtatcat tcagcgtatt
     5161 aatggttttc tgaataacct gcttgtactg acgttcatcc agctcacgtt ctttgccgca
     5221 accaataagg agaattcgct cggaaagtac attcggaaca tggtgcagca acagtgtctg
     5281 ccccggtttt ccttccagtt cgccccgacg tagcagggcg ctgatgtacc catcgctgat
     5341 tttatcgagc tgttctgcaa tcggagaaag gcgacgtggt tcgaagacgc ccacgacgat
     5401 gcaggcactc cgctgtttct ccgggctacc gctttttaca ctaaactcca tgcactacgc
     5461 tcctgaatct taaagacaac ggcggtggct acagatagaa ttgcaagctt tcgtaactca
     5521 tgtccgctgt tgcgatgact tcgtgttaat cttaacgtta ttacggcatt ggcacgtcag
     5581 aacaaaatct gagaggtgaa tccgttgagt ataattatct tagcgacgat ttcgacgact
     5641 caagagaata aatgacgttt aagccatgaa acaagctaaa atcctgcaaa agacgagttt
     5701 ttacgggcgt atttaaagtg ataatcataa gatatctggt gcgggagacg ctcaaaagcc
     5761 agctggcgat actcttcatc ttgcttttga tcttcttctg tcaaaagtta gtgaggatcc
     5821 tcggcgcagc ggttgacggc gatattccgg cgaatctggt gctctccctt ctcgggttgg
     5881 gcgtgccgga aatggcgcag cttatcctgc cattaagcct gttcctcggg ctgctgatga
     5941 cgctgggcaa actgtatacc gaaagtgaaa ttacggtaat gcatgcctgc ggtctgagca
     6001 aagcggtgct ggtgaaagcg gcaatgatcc ttgcggtgtt cacggcaatc gtcgcggcgg
     6061 ttaacgtgat gtgggcggga ccatggtcat cgcgtcatca ggatgaagtg ttagcagaag
     6121 cgaaagcgaa ccctggcatg gcggcgctgg cgcaagggca attccagcaa gcgactaatg
     6181 gcagctcggt gctgttcatc gaaagcgttg acggcagcga tttcaaagat gtgttcctcg
     6241 cacaaattcg accaaaaggt aatgcacgtc cttctgtggt ggtggccgat tccggacatt
     6301 taacccagct gcgcgacggc tcccaggtcg tcactctcaa ccagggaacg cgcttcgaag
     6361 gcactgcact gttacgcgat ttccgcatta cggactttca ggattatcag gcgatcattg
     6421 gtcaccaggc ggtggtgctc gacccgaacg ataccgacca gatggacatg cgcacattgt
     6481 ggaacactga caccgatcgt gcccgcgcag aactgaactg gcgtatcacg ttggtattca
     6541 ccgtgtttat gatggcactt atggttgtac cgttgagcgt ggttaacccg cgtcagggac
     6601 gcgtactgtc gatgctgcca gccatgctgc tgtatctact tttcttcctg atccagacct
     6661 ccctgaaatc gaacggcggt aaaggtaagc tggacccgac gctgtggatg tggaccgtta
     6721 acctgattta tctggctttg gcgattgttc tcaacctttg ggacaccgtg ccggtccgcc
     6781 gcctgcgcgc cagtttttcg cgtaaaggag cggtgtgatg caaccttttg gcgtacttga
     6841 ccgctatatc ggtaaaacta ttttcaccac catcatgatg acgctgttca tgctggtgtc
     6901 gctgtcgggc attatcaagt ttgtcgatca gctgaaaaaa gccgggcagg ggagttacga
     6961 cgcgttaggc gcaggaatgt ataccttgct gagcgtgccg aaagatgtgc aaatcttctt
     7021 cccgatggcg gctctgcttg gggcgttgct tggtcttggg atgctggcgc agcgcagcga
     7081 actggtggtg atgcaggctt ctggttttac ccgtatgcag gtggcgctgt cggtgatgaa
     7141 aaccgccatt ccgctggtct tgctgacgat ggcgattggt gaatgggtcg cgtcgcaggg
     7201 cgagcagatg gcgcgtaact accgtgcgca ggcgatgtac ggcggctcgt tgctctccac
     7261 tcagcaaggc ttatgggcga aagatggcaa caacttcgtc tacattgagc gggttaaagg
     7321 tgacgaagag ttaggtggca tcagtattta tgcctttaac gagaatcgtc gtctgcaatc
     7381 cgtacgttat gccgctaccg cgaagtttga cccggaacat aaagtctggc gtctgtcgca
     7441 ggttgatgaa tctgatctga ccaatccgaa acagatcacc ggttcgcaga cggtgagcgg
     7501 cacctggaaa accaacctca cgccagacaa actgggcgtg gtggcgctgg acccggatgc
     7561 actctccatc agtggtttgc acaactatgt gaagtatctg aagtcgagcg gtcaggatgc
     7621 cggacgttat cagctcaaca tgtggagcaa aatcttccag ccgctatccg tggcggtgat
     7681 gatgttgatg gcgctgtcgt tcatctttgg cccactgcgt agcgtaccga tgggcgtgcg
     7741 tgtggtcacc ggtatcagct tcggttttgt cttctacgta ctggaccaga tcttcggccc
     7801 gctgacgttg gtttatggca tcccgccgat catcggcgca ctgttgccaa gcgccagctt
     7861 cttcttaatc agcctgtggc tgttaatgag aaaatcgtaa tctaacaagg gagcgaaagc
     7921 tccctttatt attgttgaca aagtcaaata cgagttcgaa cggtcccctc gcccctttgg
     7981 ggagagggtt agggtgaggg gcacagatca cgtctgactt acctttttct cccccccagc
     8041 aaactcccca acatcccgcg tacaatctga ttcgtcacct ggcgagcggc gcttttcgcc
     8101 attgtttgca ccacaccatc tttcttcccg ccgcgtggtc cggtagtgcc aaacaaaata
     8161 tccttcaatc caccaagaat accgtcatcc accgccacct ctttcccttt cacgggggga
     8221 ttattttgct gctcggtact ggcctgaaag cctttttgca gcatctcata ggcggactcg
     8281 cggtccacct catcttcata tttgccatac accggggagt gattaatcag gccattacgc
     8341 tcatcttccg tcaccggccc cattcgcgaa caaggtgcga tcaccatcgc ccgttccacc
     8401 actgaaggac ttcctttcgc atcgagaaac gagattaacg cctcgccggt ccccagttcc
     8461 tggattgcct tttcggtatc aaacgccgga ttggcccgca tggtttgcgc cgctgccttc
     8521 actgctttct gatctttagg cgtaaaagca cgcaaagcgt gttgaacgcg attaccgagc
     8581 tgccctagca cgttatccgg gatatctgac gggttttgcg aaacgaacca gacgcccacg
     8641 ccttttgagc gaataagcct tatcacctgc tcaatcttat ccagcagtac ctgtggcgca
     8701 tcgttaaaca gcagatgtgc ttcgtcgaag aaaaacacca gttttggttt ctccagatcg
     8761 cccgcttcag gcaattgttc atataactct gaaagcatcc acagcaggct ggcggcgtac
     8821 agtttcggca tctgataaag tttctcggcg ctgaggatat tgataacgcc tttaccgttg
     8881 gcatcggtgc gcatccagtc tttgatatcc agcatcggct caccaaagaa gtgttctgcg
     8941 ccttgctgtt ccagcgacag taatccgcgc tggatggcac caaccgatgc gctactgata
     9001 tttccgtact gattctggaa ggatttggcg ttatcgccga tgtactgggt aatcgcccgc
     9061 aaatctttaa agtcgagcag caacaatccc tgatcgtccg caatgcggaa gataatattc
     9121 agcacgccag attgcacatc gttgagattc aacagtcgtg ccagcaacag cggccccaga
     9181 tccgaaaccg tcgcccgcac cggatggcct ttctcgccaa agatatccca caccaccacc
     9241 ggattggcat gcggttgcca gtcgttgacg ccgatatttt tcagtcttgc gagcagtttt
     9301 tccgacgccg ttcctgcctg cgcgatgccg gtcagatcgc ctttcacatc ggccataaac
     9361 accggaacac cgatttccga caatgactct gccagttttt gcaacgtgac agttttaccc
     9421 gtccccgttg cgccagtaat cagcccgtga cggttagcca ttcccggcag taaaaacagt
     9481 tctgtgtccg gcgtgcgggc aattaacagg ggttcgctca tgagatttcc tccagtttcc
     9541 tgcctggagt ataggcaacg tccggcaaag agaatatgtg taatgaagag aaaataacct
     9601 aaagggtgtt accgtgataa atctgatacc cgaggtcaac agtgttgtgg ttgtgatcgt
     9661 tattcttaat cttgctcagc aacatttggg cggccatccg accaatatca aaacggggag
     9721 taattacgct ggcgaggctt gggatcattt gcctgccaat ctccagccca tgaaaacccg
     9781 caatggaaat ttgttctggc acggcgaggt ttcgctcgcg gcacagcaga agcgcaccca
     9841 tcgcaatatc gtcattggtg caaaatacac cgtctaaatc cgggtttgcg ctaagggcat
     9901 cacgcattaa ctgcattcct aaatgaatgg atgagatggc gcgtggattc atgcgtaatg
     9961 gggaaagatt atgcagcatc atcgcatcgc aatacccctg ataacgctgt tcatcgcggg
    10021 tgtcatcttt ggaaccgaga tacagaattt tgcgcctgac tcgcttatcc agcatggtac
    10081 acaccatgtc aaaagcagcc tgccgattat caaaaccgac ctccatatcc agccgttctc
    10141 cctgtacatc catcaattcc acgaccggaa tggtggctga acgcagaaat ttcaccgtcc
    10201 tgatggtgtg atatttttcc gaaagaataa tcccgtcaat gttataagag agcaggttaa
    10261 tcaccgactc ttcttcggaa tcgcgatcgt aattgtaatt agcaataagg gtctgataat
    10321 tatgcacaga ggtgacggat tctattcccg cgagaatgtc agcgaagagt tgattttgaa
    10381 atgacggaat caagataccg agggtataac tttgtgcatt caacaacatg ccgggagcgc
    10441 gattagggat ataattgatc tcttccataa ttttggcgat gcgctcgcct gtttcctttg
    10501 ccaccttttt cggcgagcgg atataacgac tcacggtcat ttttgttacg ccagccagcg
    10561 tagcgatatc ctgtaaagaa attctgtgat tgcgcataac ttctcccaac gtcgggggaa
    10621 tcttcaatct tcccccgaaa ctacatcaca atattttatt ctttcagtgc aatacggcgt
    10681 taatggctaa cacgcccagc aaacccataa tagaaataag ggtttccatc actgtccagg
    10741 tacgcaacgt ttcaccaacc gtcagattaa aatacccttt aaatagccag aagccagggt
    10801 cgtttacgtg tgacgcgatc acgctgcccg caccagttgc cagtaccatt aaagcgggat
    10861 cggcatgggt aacgttgata atcggcaaca ccacacccgc ggtggtaatg gccgcgacgg
    10921 tggcagagcc cagagcgata cgcaacagcg ccgcgacagt ccagcacatc aataacggcg
    10981 aaagcgtagt tccggtcatt aagtgtgaaa tatactgccc gacaccacta tctactaata
    11041 cctgcttaaa cgcgccgccg ccagcgataa taaacacaat catcgcgata gcgcctatag
    11101 aatccccaat gatatccatg atttgctcga tggtgcgtcc attacgtcgg cccagtgtga
    11161 aaatcgcaat aacaatggca ataaacagcg caacggcagg gttaccgaca aattcaaaga
    11221 agaggcgcac ggtgttagtt ttcggtaacg taatttcaca aacggcggcg atagccatca
    11281 ggatgaccgg gatcactgca gcaaaaatac tgttccagaa ggagggcatc tcctcttcgc
    11341 taaacagatg aggattaaat aagccttccg gtggtgcttt ctcaaagcga gttagcagtt
    11401 tagaaaacag cggtcctgcg acaataactg tcggaatggt aatgataaat ccatacagta
    11461 aagtcgttcc gagattagcc tcaaagatag tcgcgatggc agtaggccct ggatgtggcg
    11521 gcagaaaaca gtgggttaca gagagcgctg ctaccatcgg tacgccaaca tacaacaggg
    11581 gtaatccaga tgatgccacg atggtaaata ccaacggcaa cagcaggaca aaacccactt
    11641 caaaaaacat ggcgaggccc acaaccagac cggtgatcac tagcgcccat tgcacgcgtt
    11701 ttttaccaaa agtagcaatc agcgtagtgg cgatacgttg tgccgcgccc gtatcagaaa
    11761 tcagcttgcc taacatggcc ccgaaaccga ggatcattgc cagccctccg agcgtgctgc
    11821 cgataccatt ttgtatagag tgcaggacgg cctgtgcatc catcccttcg gcaaatccga
    11881 cgacggcagc taccagaacg agggcaataa agccgttaac tttaaagccg atcatcagga
    11941 taagaagcag cgcgacgcct gccgcaataa tgattaatgg catagtcttt atctcttatt
    12001 agtgccagaa aagcagcgct ttcgcgctgc acgtagggtg gtggctgtta aacagccact
    12061 aacatgccgc catcaacaaa caacaggtgg ccgtttacga aatcagaggc ttttgaagaa
    12121 aggaacaccg cagcaccaat cagttcctgc ggatctcccc agcgtgcggc gggggtccgt
    12181 ttgcacaacc aggcggtgaa ggcttcgtcc tcaaccagtg ctttagtcat ttctgttttg
    12241 aaatagcccg gcgcaatacc gttgacctga atattgtggc gcgccagctc gacgcacatg
    12301 ccgcgggtga gcatttttac cgcccctttc gatgcggcat aaggggtgat ggtgtcacgt
    12361 cccagttcgc tttgcatcga gcaaatatta ataactttac ctgccttgcg ttcaaccatg
    12421 tgacgagtta ccgcttgcga taccaggaac acggcggtct ggtttactgc gatcacatca
    12481 ttccactctt gttcagggaa ctcagtaaaa ggatgacggc gctggatacc ggcgttattc
    12541 accagcacat caatggggcc gatgtccttt tcgatatgtt caacggcggc atcaatttca
    12601 tgtttatgag taacattaaa aggtgcggca acggcctgaa taccctcctg gtggagtttt
    12661 tctacagcaa gttctgcgcg ttcggcagta atatcattaa taattatttg tgcgccatat
    12721 ttacccaggc cggttgccag taaatagcca atgccctgtg ctgaaccggt aatcaagata
    12781 ttttttcctg ccagtgaaaa tagatcgttc attgtattat tcctttattt atctcttaga
    12841 aaacaagctg gacttttgct gcctgggttt tatcaccggc gaaacgtagc gcctcttcca
    12901 ggtcagtgaa gagatattca gcactcagta aaggcagtgg attgataacg ccattcgcca
    12961 gccatgacac tgcggtatta aattcgctgg taaaacggaa agagcctttg agtgaaatct
    13021 ccttaccaat caacgtcatc attgggaatt ctgccatcgc gcctcccatc cctacctgca
    13081 ccattacgcc gcgtgcacga gtgacctcca gacaggtatt cactgatgaa ggatgaccgg
    13141 acacttcaaa gctgacatcg aaatggcctt tttccgcttt ccagtgatcc atgtcgtcgt
    13201 tttgtgggtt tacgagcaca tccgcaccca tctctttggc cagcgaaagg gaacggggac
    13261 tcacatcggc acagacaatt tccgcggacc ccagtgtttt cacagcactg acaatcaggc
    13321 agccaatggg cccaacaccg gaaacaaata ctcgcttgcc ctgtaactcg tcggcctgat
    13381 gtgcggcatg aatcgcgacg gctaaaggtt cggcaaaagc cataaccttt tcgtcagctt
    13441 tggccggata agggacacat tgcgacgttt cgaccatttt ataacgggta aaaccaccat
    13501 caacatgagg gaaatacatg gcactgccaa aaaaacgcat atctgtacac tgattctcgt
    13561 tatgttcaat gcagtatttg cagtgaccgc acggtttaga cggattaatg gctaccgttt
    13621 gcccttcatg taattctgat gagtcgctat gaataacttt accgataact tcatgaccta
    13681 acaccatcgg tgcctttatc atgaaattac ctacttttcc ttcctgataa taatgtaaat
    13741 cggaaccgca aattccacct cgggttattt gtactaatgt tccattatta ttccaatcta
    13801 tcgtctgctc ggtaacggca acagttttct tgtccgcaac aacgcaggac tgtgttttca
    13861 cttgcataaa ttcctcactg gtcaggtaga cacctcggaa gcatttaagc ggttttaatt
    13921 gtcattattt gtgatgaaga tcacgtcaga aaattgttac attactatgt tacgcataac
    13981 gtgatgtgcc ttgtaattct tatcagcaga aataaaaaaa cgtgaaatta ttatgccacc
    14041 aggcgtagta tcgcagcagg taagatgatt caggagattt taaatggcgg gtgaaagctt
    14101 tattttgatg ggcgtttcag ggagtggtaa aacattaatt ggtagcaagg ttgccgcgtt
    14161 attatctgct aaatttattg atggtgacga tcttcatcca gccaaaaata tagataaaat
    14221 gtcgcagggt attccattat ctgatgaaga tcgacttccc tggctggaac gcttaaatga
    14281 tgcttcatac agtctttata aaaagaatga aacaggattt attgtctgtt catcattaaa
    14341 aaaacagtat cgtgatattt tacgcaaggg tagcccccat gttcatttcc tctggttaga
    14401 tggcgactat gaaactattc tcgcgcgaat gcagcggcgg gctgggcatt ttatgccggt
    14461 agcgttacta aaaagtcagt ttgaggcgct ggagcgtcca caagcagatg aacaggatat
    14521 tgtacgcatt gatatcaacc atgatattgc caatgtcacc gaacaatgtc ggcaggccgt
    14581 gctggcgata cgacaagaca gaatatgtgc gaaagggggt agcgcctcag atcagcgctg
    14641 cgaatgattt tcaaaaatcg gctttcagaa ccacgcggta acgcgcctta ccgttgcgca
    14701 catgctggat ggcgtcgttt attttcgaca tcgggaacag ttcggtggtc ggcgcaacct
    14761 tgctgcgggc cgcaaagcgc atcagcttac gaagctcata aggtgtgccg gttgctgaac
    14821 cagagacgct gcggtcgccc gcaattaacg taaaggccgg aacagacagc ggcgtgagga
    14881 ccgcaccgac ggtgtggaaa ttaccgccat acgtcagcgc ctcaaaataa ggctgccagt
    14941 cgaggctgac gttgacggtg ttgataatga gatcgaactg ccccgcaagt gttttcagtg
    15001 cctgcggatc gcggctattc accactttat cggcacccat cgccagtact tcctgctctt
    15061 tcgcaggatt cgaactaaag gctgtcacct cgcatcccat tgcgtgcaga agttttatag
    15121 cgatatgccc cagcccgcca ataccaatta ccccaacgcg gctggtagca gtgatatggt
    15181 gcatcaacag tggtttaaag accgtgatac caccacacag cagcggcccg gcggactcga
    15241 tatcgatatt ttccggcagt gggatcaccc attgccagtc tgcacgcaac ttctcggcaa
    15301 aaccgccgcg attcataatt gttggcaccg caccttgctc gcagttgatc tgattaccgc
    15361 taatacaggc gtcgcaatgc ccgcagctac gcgccgtcca gccaatcccg acacgctgac
    15421 cgacctgcaa acctttatcc tgcgcggcgc tcccgagcgc caccacgcgc ccaatcacct
    15481 catgcccggc aaccagcgga tactgtgaaa atccccattc gttgtcgatc atcgacagat
    15541 cggaatggca gatcccgcag taatccacct gcacttcaac atcttgtggc ttcaattcac
    15601 cgggctcgta ctcataaact tccagttcgc cgcccgcttc ttttgcggca tagcttttta
    15661 tcatcgacat tttttggtcc ttctctggtg ttgtctggga agtgtagagc atggcagggc
    15721 ggggtgcctg gagtgtgaca aaggttacac atcgctgtat gcaatgctga aaatttcagc
    15781 acttagcgag gtgcgagcaa gctggcgctt gcatggtggc gtgcgacagg tataatccac
    15841 aacgttttcc gcatacctct tcagtgccga agtggcgaaa tcggtagacg cagttgattc
    15901 aaaatcaacc gtagaaatac gtgccggttc gagtccggcc ttcggcacca aaagtatgtc
    15961 aatagacctc aactgaggtc ttttttatgc ctgaaattca gtatttatct acctttcccg
    16021 ctatattaac tctctcaagg tcaaccgata tcaacgtact tctaccaaca tatgttggta
    16081 cagatgatgg tatttccggt tcgataatgc ttgtaccaac agggagggaa tacgcatggc
    16141 attaacagac atcaaagtca gaacagctaa gccaacagat aagcaatata agttgactga
    16201 tggtggcggt atgcatctgc ttgtccatcc aaatggttct aagtactggc gtttgcagta
    16261 ccgttatgag ggaaagcaaa agatgctggc acttggggtt tatcctgaaa tcacactcgc
    16321 ggatgccaga atacgtcgtg acgagccacg taagctgctt gcgaatggcg tcgatccggg
    16381 agacaaaaag aaaagtgata aggttgaaca gagtaaagca cgaaccttta aagaagtcgc
    16441 gattgagtgg catggcacca ataaaaagtg gtctgaagat catgcccatc gtgtgctaaa
    16501 aagtctggaa gataatcttt ttgcagcgct tggtgaacgt aatatcgctg agttaaaaac
    16561 tcgagattta ttagcaccta ttaaggccgt agaaatgtct gggcgtcttg aaatggctgc
    16621 tcgtctt
//
LOCUS       DAJTYZ010000089        16224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000089 DAJTYZ010000000
VERSION     DAJTYZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16224
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.089"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            79..2394
                     /gene="bisC"
                     /locus_tag="OO596_002787"
     CDS             79..2394
                     /gene="bisC"
                     /locus_tag="OO596_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6718602.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2363..2803)
                     /locus_tag="OO596_002788"
     CDS             complement(2363..2803)
                     /locus_tag="OO596_002788"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6718603.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2800..3363)
                     /gene="tag"
                     /locus_tag="OO596_002789"
     CDS             complement(2800..3363)
                     /gene="tag"
                     /locus_tag="OO596_002789"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6718604.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3521..4219
                     /locus_tag="OO596_002790"
     CDS             3521..4219
                     /locus_tag="OO596_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6718605.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4448..5656
                     /locus_tag="OO596_002791"
     CDS             4448..5656
                     /locus_tag="OO596_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6718606.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5980..7671
                     /gene="eptB"
                     /locus_tag="OO596_002792"
     CDS             5980..7671
                     /gene="eptB"
                     /locus_tag="OO596_002792"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6718607.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7763..7839
                     /locus_tag="OO596_002793"
     tRNA            7763..7839
                     /locus_tag="OO596_002793"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7797..7799,aa:Pro,seq:cgg)
     gene            7874..8007
                     /locus_tag="OO596_002794"
     ncRNA           7874..8007
                     /ncRNA_class="other"
                     /locus_tag="OO596_002794"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8752..10359
                     /gene="dppA"
                     /locus_tag="OO596_002795"
     CDS             8752..10359
                     /gene="dppA"
                     /locus_tag="OO596_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6718608.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10667..11686
                     /gene="dppB"
                     /locus_tag="OO596_002796"
     CDS             10667..11686
                     /gene="dppB"
                     /locus_tag="OO596_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6718609.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11696..12598
                     /gene="dppC"
                     /locus_tag="OO596_002797"
     CDS             11696..12598
                     /gene="dppC"
                     /locus_tag="OO596_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6718610.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12609..13592
                     /gene="dppD"
                     /locus_tag="OO596_002798"
     CDS             12609..13592
                     /gene="dppD"
                     /locus_tag="OO596_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6718611.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13589..14593
                     /gene="dppF"
                     /locus_tag="OO596_002799"
     CDS             13589..14593
                     /gene="dppF"
                     /locus_tag="OO596_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6718612.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14623..15894)
                     /locus_tag="OO596_002800"
     CDS             complement(14623..15894)
                     /locus_tag="OO596_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6718613.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3852 a         4047 c         4364 g         3961 t
ORIGIN      
        1 attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa tacatctaac
       61 ctttcaggag tcttcggttt ggccaactca tcctcacgat attccgttct gactgccgcc
      121 cactgggggc ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc gcgtggcgcg
      181 ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt tcacagcaat
      241 acgcgggtac gatttccaat ggtgcgcaaa ggctttctcg cgtcaccgga aaacccgcaa
      301 ggcattcgtg ggcaggatga atttgttcgc gtgagttggg atgaggcgct ggatcttatt
      361 caccaacaac ataaacgcat tcgtgaggct tatggtccgg catcgatttt tgctggttcc
      421 tacggctggc gttcaaacgg cgtgctgcat aaggcctcga cattattaca acgctatatg
      481 gcgctggcag gcggttatac cgggcatctg ggggattatt cgaccggtgc ggcacaggcg
      541 atcatgccgt atgtcgtagg tggcagcgaa gtttatcaac agcaaaccag ttggccgctg
      601 gtgctggaac atagcgatgt cgtggtgttg tggagtgcta acccactcaa tacgctgaaa
      661 attgcgtgga atgcatccga tgagcagggg ctttcttact tttccgcact gcgtgacagt
      721 gggaaaaagc tgatctgcat tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat
      781 aaaatggagt gggtggcacc gcacatgggc accgatgttg cgctgatgct ggggatcgcc
      841 catacgctgg tggaaaatgg ttggcacgac gaagcgtttc tggcgcgttg caccacaggt
      901 tatgccgtct tcgcctctta tttgctgggc gagagtgacg gaatagcgaa aaccgccgaa
      961 tgggcagcag agatttgtgg tgttggcgca gcgaaaatcc gccagctggc ggctattttc
     1021 caccaaaata ccaccatgct gatggcaggc tggggaatgc agcgccaaca gtttggtgag
     1081 caaaaacact ggatgatcgt cacgctggca gcaatgttgg ggcaaatcgg cacacccggc
     1141 ggcggttttg gtctttctta ccattttacg cggcgttctg cggtgctctc ttccatgcag
     1201 ggcagcttgc cgggtggctg cgatgcggtg gataaaatcc ctgttgcccg cattgttgaa
     1261 gcactggaaa accctggtgg cgcatatcaa cacaacggta tgaaccgaca tttcccggat
     1321 attcgtttta tctggtgggc gggcggtgcc aactttactc atcatcagga taccaatcgt
     1381 ctgatccgtg cctggcaaaa accggagctg gtggtgatct ctgaatgctt ctggacggcg
     1441 gcggcgaaac acgcggatat cgttctgcct gcgactacct cttttgagcg taatgatctc
     1501 accatgaccg gtgattacag taatcagcat ctggtgccga tgaagcaagt ggtgccgcca
     1561 cgctatgaag cgcgtaatga ttttgatgtt tttgccgagt tgagtgaacg ttgggagaag
     1621 ggcggctatg cgcggtttac ggaaggaaaa agtcagctgc aatggctgga aacgttttat
     1681 aacgttgccc ggcagcgcgg ggcaagccag caggttgaat tgccgccatt tgctgagttc
     1741 tggcaagcca accagttaat tgagatgccg gaaaacccgg acagcgagcg gtttattcgc
     1801 ttcgctgatt tttgccgcga tccgctggcg catccgttaa aaaccgccag cggcaagatt
     1861 gaaatcttct cacagcgtat tgccgattac ggttacccgg attgccctgg gcatccaatg
     1921 tggctggagc cggacgaatg gcagggcaat gccgaaccag aacagttgca ggtactttct
     1981 gcccatccgg cgcaccgcct gcacagccag ctgaattaca gttctctgcg cgaattgtac
     2041 gcggtagcaa accgtgagcc tgtcaccatt catcctgacg atgctcaggc gcgcggcata
     2101 accgaagggg atatggtgcg ggtgtggaac tcccgtgggc agatccttgc cggagcggtc
     2161 attagcgagg gaattaaacc tggcgtgatt tgcattcacg aaggggcatg gccggatctg
     2221 gatttaaccg ctgacggtat ttgtaaaaac ggcgcagtga acgtgctgac caaagatctc
     2281 cccagctcgc ggctggggaa tggctgtgcg ggtaatacgg cgctggcatg gctggaaaaa
     2341 tacaacggtc cggaactgac acttacagcg tttgaaccac cggccagctc ataatccagg
     2401 tgggtagttg ggtttcatcc tgccatgcgc aatcgacaat gtgaaaaccc tgcgcacggt
     2461 aaaaatctat cgccggttga tttttttgat aaacctccag catcagatgg ggatagcgct
     2521 gctgcacata ctgcatcagc gccttaccaa taccgcgtct gacggccttc ggtgcgacaa
     2581 acattgctgc cagaaatcgg ccttccataa tgctgacaaa accgagaagc ttaccgtcct
     2641 cttcccagac ccagttttgc gcgttggcaa gataggcatc ccgcaccagc ggaatgcagt
     2701 cacgccagta attcgctttt ataaagggat gcccccaggt tgtactttcc agccacagtt
     2761 cgaggatcgc ggggagttct gaacgttgcg cttcccgaat catggtttat ttcccggata
     2821 gcaacagcag ccaaccacat gatcattcac cagcccacat gcctgcataa aggagtaaca
     2881 gattgtggtg ccgacaaact taaaaccacg ttttttcagt gctttagata gggcgtcgga
     2941 ggcggacgta gatgtgggaa tttcgctcaa cgttgtggct tgtgtcacct gtggctgatg
     3001 atttacaaac gaccagacaa agtcggcaaa cggttcgccg ttctgttcca tttgcaggta
     3061 cgcccgcgca ttaccaataa ttgcctgaat tttccctcga tggcggataa tcccggcgtc
     3121 ctgtaccagt ctttcgacat cctcttcctg cattgctgcg accttcaccg gatcgaactg
     3181 atgaaagcag gcgcgatagt tttcgcgttt tttgaggacg gtgatccacg ataacccagc
     3241 ctgctgccct tcaaggcaga tcatttcgaa cagtttttta ctgtcagttt caggcacgcc
     3301 ccattcatta tcatggtagg caatataaag cgggtcctga ctcacccagc cgcaacgttc
     3361 catactttcc ctcgctatgt acccgatcgt aaaaatttca gccgcttttc ttgacgcatc
     3421 ggtgaaaaag tcaatattta atacagggct actatgcccg atatccttca tacaatgaca
     3481 ataatattgc cgaattcggc tcttccctgg agtcgtgtta atgatcataa aaaaaagcgg
     3541 tggtcgctgg cagctaagcc tgctggcgag cgtggtaatc agtgcctttt ttctcaacac
     3601 agcttacgcc tggcaacaag aatatatcgt tgatacgcaa cccggacatt ccacagagcg
     3661 ttacacctgg gatagtgatc atcaacctaa ttacaacgat attttgtcgc aacgtattca
     3721 aagtagccaa agggcgctgg gactggaagt caatctggcg gaagaaactc ctgtggatgt
     3781 gaccagcagt atgagtatgg gctggaattt tcctttatat gaacaggtta caaccggccc
     3841 ggtcgcggca ttacattacg atggcacaac cacctcgatg tataacgagt ttggcgacag
     3901 tactaccacg cagaccgatc cgttatggca tgccagcgtg agtaccttag gctggcgtgt
     3961 tgactcccgg cttggcgatc tccgaccctg ggcgcaaatc agctataacc agcaatttgg
     4021 cgagaatatc tggaaggcgc aatcaggcct gagccggatg acggcgacaa accagaacgg
     4081 caactggctg gatgtcaccg taggcgctga tatgttgctc aatcaaaata ttgccgccta
     4141 tgccgcgcta actcaggcag aaaataccac taataatagc gactatctgt atacgatggg
     4201 ggttagcgcc agattttaac gtaacagtca caattgaaac cattaaataa caataattgt
     4261 ggcgatcgtg ggtgctaact taccaaataa taaatttggt gaataattgt cgcgtcattc
     4321 attcctgaac taaggcattt cattccgttc tgatggcatt tcatgccgtt tttccccagg
     4381 cataaagtgc acttcgttat ggttgtcggc agagattttt cctttttatt actgcaggaa
     4441 tactgccatg acaccttcaa attatcagcg tacccgctgg ctgacactca tcggtactat
     4501 cattacccag tttgcgctgg ggtcggttta tacctggagc ctgtttaatg gcgcgctttc
     4561 cgccaagctg gatgcgccgg taagccaggt cgctttctct ttcggcttgt taagtctggg
     4621 gctggcaatt tcgtcttctg ttgcgggcaa attacaggaa cgtttttgcg ttaaacgcgt
     4681 caccatggct tccggcattt tgctgggatt aggcttcttc ctgaccgcgc attccaacaa
     4741 cctgatgatg ctgtggttaa gcgccggtgt gctggtcggt ctggcggatg gcgcgggtta
     4801 cctgctgacg ctctctaact gcgtgaagtg gttcccggag cgtaaaggtc tgatctccgc
     4861 gttcgctatc ggttcttatg gtctgggtag cctgggtttc aaatttatcg acacgcagct
     4921 gctcgaaacg gtgggtctgg aaaaaacatt tgtgatttgg ggagcgattg cgctggtgat
     4981 gattgttttc ggcgcaacgt taatgaaaga cgcaccaaaa caggaagtga aaaccagcaa
     5041 tggtgtggtg gagaaagatt acacgctggc agagtcgatg cgtaaaccgc agtactggat
     5101 gttagcggta atgttcctga ctgcctgcat gagcggcctg tacgtgattg gggtagcgaa
     5161 agatatcgcc caaagtctgg cacaccttga tgtggtttcc gcagccaatg cagtaacggt
     5221 tatttccatc gctaaccttt caggtcgtct ggtgctgggc attctgtctg acaaaattgc
     5281 ccgtatccgt gttatcacca ttggtcaggt gatttcgctg gtgggtatgg cggccctgct
     5341 gtttgcacca ttaaatgcag tgacgttctt tgcagcgatt gcctgcgtgg catttaactt
     5401 tggcggcact attaccgtct ttccatcact ggtcagtgag ttcttcggcc tcaataacct
     5461 ggcgaaaaac tacggtgtga tttacctcgg tttcggtatc ggtagcattt ttggttcgat
     5521 tatcgcctca ctgtttggcg gtttctatgt gaccttctac gtgattttcg ccctgctgat
     5581 tctgtcattg gcgctttcta cgacgattcg tcagccagag cagaaaatgt tgcgtgaggc
     5641 gcatggctcc ctttaatgct cagccgcgag ctactgggct tcagcttccg tgtgcatcaa
     5701 acgcatcaac gaaatgtata aacaatgact cgtaaaattt gggccagtta actggccctt
     5761 tttactgctt tttgtaacgc taaagtctct tttcaaactt gcatttttgt aaatttgtgc
     5821 ttcatgcaca ctctttcccc acactttttc gctttgctgt ggtctactta ttcgcgcgtg
     5881 tagattttac ttatctgact acctccgcac ttttcccctg ccgggcctga aaagccgtta
     5941 agcagggtgt tatcacctgt ttgtccaggg tttgtttgca tgagatacat caaatcgatt
     6001 acacagcaga agctgagctt tttgcttgca atctatattg gcctttttat gaatggcgcg
     6061 gttttttatc gccgcttcgg cagctatgcg cacgatttta ccgtctggaa aggcgtttct
     6121 gctgttgttg aactggccgc caccgtactg gtgaccttct ttttactacg tcttctatcg
     6181 ctgtttggcc gccgcagctg gcgtattctg gcgtcgctgg tggtgctctt ttccgcaggt
     6241 gccagctatt acatgacctt ccttaatgtg gtcattggtt atggcatcat cgcttccgtc
     6301 atgaccaccg atatcgacct gtcaaaagaa gttgttggtc tgaactttat tctctggtta
     6361 atcgccgtta gtgcattgcc tcttatcctt atctggaata accgctgtcg ctacaccttg
     6421 ctccgacaac tgcgaacccc agggcagcgt attcgcagcc tggcggtcgt cgtactggcg
     6481 ggtattatgg tttgggcacc gattcgtttg ctggatatcc agcagaagaa agtggagagg
     6541 gcgaccggcg ttgatttgcc gagttatggc ggtgtcgtag cgaactctta tctgccatca
     6601 aactggcttt ctgcgttggg gctgtatgcc tgggcgcggg tcgatgaatc ttccgataat
     6661 aattcattgc ttaatccggc gaagaagttt acataccagg ctccacaaaa cgttgatgag
     6721 acttatgtcg tgtttatcat cggtgaaacc acgcgttggg accatatggg tattttcggc
     6781 tatgagcgta ataccacgcc gaaactggcc caggagaaaa atctggcggc gttccgtggt
     6841 tactcctgtg ataccgcaac caaactctca ctgcgttgca tgtttgtacg tcaggggggc
     6901 gcggaagata atccgcagcg cacattaaaa gaacagaaca ttttcgcggt tctgaagcag
     6961 ttaggattca gttctgacct ctacgctatg cagagcgaaa tgtggttcta cagcaacacg
     7021 atggcggaca acattgctta tcgtgagcag attggtgcgg agccacgtaa tcgtggcaag
     7081 ccggtagatg atatgttgct ggtagacgaa atgcagcaat cgctagggcg caacccggat
     7141 ggtaagcatc tgatcattct gcataccaaa ggttcgcatt ttaactacac ccagcgttat
     7201 ccgcgtagct tcgcgcagtg gaagccggaa tgtattggtg ttgatagcgg ctgtaccaaa
     7261 gcgcagatga tcaactccta tgacaactcg gtgacctatg tggatcactt tatctccagc
     7321 gtgattgatc aggttcgcga taagaaagcg attgtgttct acgcagctga ccacggtgag
     7381 tcaattaatg aacgcgagca cctgcacggc acgccgcgtg aactggcacc gccggagcag
     7441 ttccgcgtac cgatgatggt ctggatgtca gataaatatc tggaaaatcc ggccaatgcg
     7501 caggcgtttg cgcagctgaa aaaagaagcc gacatgaaag tgccacgccg tcacgtagag
     7561 ctgtacgaca ccatcatggg ttgtcttggc tatacttcac cggatggtgg aattaacgaa
     7621 aacaacaact ggtgtcacat cccgcagaca aaagaggcag cggctaacta agaggcctgc
     7681 tgactttctc gccgatcaaa aggcattttg ctattaaggg attgacgagg gcgtatctgc
     7741 gcagtaagat gcgccccgca ttcggtgatt ggcgcagctt ggtagcgcac ttcgttcggg
     7801 acgaaggggt cggaggttcg aatcctctat caccgaccaa attcgaaaag cctgctcagc
     7861 gagcaggctt ttttgcattt gaagcccatg aggatgagaa cctccggggg cggggaggtt
     7921 cgactcgagc gaagcgagag aacgttgcgc cagcaacggc ccgcagggcg agccacgaag
     7981 tggcgagtaa tcctctatca ccgaccaaat tcgaaaagcc tgctcaacga gcaggctttt
     8041 ttgcatttgt agctcatgag gatgagaacc tccgggggca gggaggttcg actcgacgta
     8101 ggcctgataa gacgcgccag cgtcgcatca ggcaacggca ccgcatttat tgtcggatgc
     8161 ggcgcgaccg ccttattcga ccaacacacc acacattttc ctgccaactt tgcgccccat
     8221 atctcactat ccattaacgg atttgtgaca gaaaccattg tcttttttta tatttgctta
     8281 aatctttttt tgcgttactt atggctggcg ctggcatttt ccgctgtgcg ttttagcgtt
     8341 catggtatta atcgctcaga tatccacctt ttcctcagat ttttttcatt aaatgcataa
     8401 aacttaatca ccaaatgttg ctaaatatta agctgattgt tctggttatg ttggagtagc
     8461 acaaatttct ctgctgcaaa tagctgtttg aagttttttt aatctttgtt tgtggattct
     8521 caccgttggt gtaaatcccg gttggctgta ttgacgtttt cacattctgt tgacagattg
     8581 taggtcacga ggggcatttt atggaggatc cgcactgtta cactgatgtt aattagtacg
     8641 gcatccccac ctcataacgt tgacccgacc gggcaaaaaa caaaaaaggt caggcagcga
     8701 caacccactg caaagggtta aaacaacaaa catcacaatt ggagcagaat aatgcgtatt
     8761 tccttgaaaa agtcagggat gctgaagctt ggtctcagcc tggtggctat gaccgtcgca
     8821 gcaagtgttc aggctaaaac tctggtttat tgctcagaag gatctccgga agggtttaac
     8881 ccgcagctgt ttacctccgg caccacctat gacgcctctt ccgtaccgct ttataaccgc
     8941 ctggttgaat ttaaaatcgg caccactgaa gtgatcccgg gcctcgctga aaagtgggaa
     9001 gtcagcgaag acggtaaaac ctataccttc catctgcgta aaggtgtgaa gtggcacgac
     9061 aataaagaat tcaaaccgac gcgtgaactg aacgccgatg acgtggtgtt ctcgttcgac
     9121 cgtcagaaaa acgcgcaaaa cccgtaccat aaagtctctg gcggcagcta cgaatacttc
     9181 gaaggcatgg gcttgccgga gctgatcagt gaagtgaaaa aggtggacga caacacagtt
     9241 cagttcgtac tgactcgccc ggaagcgccg ttcctcgctg atctggcaat ggacttcgcc
     9301 tctattctgt caaaagaata tgctgatgcg atgatgaaag ccggtacacc ggaaaaactg
     9361 gacctcaacc caatcggaac cggtccgttc cagttacagc agtaccaaaa agattcccgt
     9421 atccgctaca aagcgtttga tggctactgg ggcaccaaac cgcagatcga tacgctggtt
     9481 ttctctatta cccctgatgc ttccgtgcgt tacgcgaaat tgcagaagaa tgaatgccag
     9541 gtgatgccgt acccgaaccc ggcagacatc gctcgcatga agcaggataa atccatcaac
     9601 ctgatggaaa tgccggggct gaacgtcggc tacctctcct ataacgtgca gaaaaaaccg
     9661 ctggatgacg tgaaagttcg ccaggcactg acctacgcgg tgaacaaaga cgcgatcatc
     9721 aaagcggttt atcagggcgc gggtgtatca gcgaaaaacc tgatcccgcc aaccatgtgg
     9781 ggctataacg acgacgttca ggattacacc tacgatcctg aaaaagcgaa agccttgctg
     9841 aaagaagcgg gtctggaaaa aggtttctcc atcgacctgt gggcgatgcc ggtacaacgt
     9901 ccgtataacc cgaacgctcg ccgcatggcg gagatgattc aggcagactg ggcgaaagtg
     9961 ggcgtgcagg ccaaaatcgt cacctacgaa tggggtgagt acctcaagcg tgcgaaagat
    10021 ggcgaacatc agacggtaat gatgggctgg actggcgata acggggaccc ggataacttc
    10081 ttcgccaccc tgttcagctg cgccgcctct gaacaaggtt ctaactactc aaaatggtgc
    10141 tacaaaccgt ttgaagatct gattcaaccg gcgcgtgcta ccgacgacca taacaaacgc
    10201 gttgaactgt acaagcaagc gcaggtggtg atgcacgatc agactccggc actgatcatc
    10261 gctcactcca ccgtgtttga accggtacgc aaagaagtca aaggctatgt ggttgatcca
    10321 ttaggcaaac atcacttcga aaacgtctct atcgaataat taaaagccat acaagactga
    10381 tggcaaaggc aaaactgcct gatgtgctcc gcttatcagg cctacgaaaa ttctgcaatg
    10441 tattgaattt gcacgatttt gtaggccgga taaggcgtta acgccgcatc cggcataaac
    10501 aaagcgcact ttgtcaacaa tctgtatacc cggtggcgat gtgcctctgc gtgcagcgcc
    10561 atccggcagc aatacttcat tccctgtccg ataagggcgg gaattgattt gtgagcaata
    10621 cagacacgca gttccaggct gcgggtcact atagagaatc cgggttatgt tgcagtttat
    10681 tctccgacgt ttgggactcg tcatccccac gtttatcggt attacccttc tcacatttgc
    10741 ctttgtccac atgatcccgg gcgatccggt gatgatcatg gcaggcgaac gcgggatctc
    10801 ccctgagcgt cacgcgcagc tgctggctga actcggctta gataaaccga tgtggcagca
    10861 gtatctccat tacatttggg gcgtaatgca cggcgatcta ggcatttcaa tgaaaagccg
    10921 aattccagta tgggaagagt tcgtgccgcg ctttcaggcc acgctggaac ttggcgtctg
    10981 cgcgatgatt tttgctacgg cagtcggtat tccggttggt gtgctggctg cggttaaacg
    11041 cggttccatt ttcgatcaca ctgcagttgg cctggcgctg accggctact cgatgccgat
    11101 cttctggtgg ggcatgatgc tgatcatgct ggtttcggtg cactggaacc tgacgcccgt
    11161 ctccggtcgc gtgagcgata tggtgttcct cgatgactcc aatccgttaa ccggttttat
    11221 gctgatcgac accgccatct ggggtgaaga tggcaacttt atcgacgccg ttgcccatat
    11281 gatcttaccg gctattgtgc tgggtactat cccgctggcg gttatcgttc gtatgacgcg
    11341 ctcttcgatg ctggaagtgc tgggtgaaga ttacatccgc accgcgcgcg ccaaagggtt
    11401 gacccgcatg cgggtgatta tcgtccatgc gctgcgtaac gcgatgctgc cggtagtgac
    11461 cgttatcggc ctgcaggtgg gaacattgct ggcgggggcg attctgaccg aaaccatctt
    11521 ctcgtggccc ggtctgggac gctggttgat tgacgcactg caacgccgcg actatccggt
    11581 agtgcagggc ggcgtattgc tggtggcgac gatgattatc ctcgtcaacc tgctggtcga
    11641 tctgctgtac ggcgtggtga acccgcgtat tcgtcataag aagtaagggg acatcatgtc
    11701 acaggttact gaaaataaag tgattagcgc accggtgccg atgaccccgt tacaggagtt
    11761 ctggcactat tttaaacgca acaaaggcgc ggttgtcggg ctggtttaca tcgtcatcgt
    11821 gctgtttatc acgatctttg ccaactggat tgcgccttat aacccggcgg atcagttccg
    11881 cgatgcgctg ctcgccccgc cagcctggca ggaaggcggc agcatggcgc acttgctggg
    11941 caccgatgac gtaggccgtg atgtgctgtc gcggctgatg tacggcgcgc gcttgtcgct
    12001 gctggttggc tgtctggtgg tggtgttatc gctgattatg ggcgttattc tcggcctgat
    12061 cgccggttac ttcggcggcc tggtcgataa catcatcatg cgcgtggtcg atatcatgct
    12121 ggcgctgcca agtctgctgt tggcactggt gctggtggcg attttcggcc cgtcgattgg
    12181 taacgccgcg ctggcactga ccttcgttgc cttgccgcac tatgtgcgct taacccgcgc
    12241 cgccgtgctg gtggaggtga atcgcgatta cgttaccgcg tctcgcgtgg caggtgccgg
    12301 ggcgatgcgt cagatgttta ttaacatctt cccgaactgc ctggcgccgc tgattgttca
    12361 ggcgtcgctc ggtttctcta acgccattct cgatatggcc gcccttggct tcctcggcat
    12421 gggagcgcag ccgccaacac cagagtgggg caccatgctc tccgacgtgt tgcagttcgc
    12481 gcaaagcgcc tggtgggtcg tgaccttccc gggtctggcg atcctgctga cggtgctggc
    12541 atttaacctg atgggtgacg gtctgcgtga cgcgctcgat cccaaactga agcagtaaga
    12601 ggttcgagat ggcgttatta aatgtagata aattatcggt gcatttcggc gacgaaagcg
    12661 caccgttccg cgccgtagac cgcatcagct acagcgtaaa acagggcgaa gtggtcggga
    12721 ttgtgggtga gtctggctcc ggtaagtcgg tcagttcact ggcgattatg gggctgattg
    12781 attatccggg ccgcgtaatg gcggaaaagc tggtgtttaa cggtcaggat ttgcagcgta
    12841 tatcagaaaa agagcgccgc aacctggtgg gtgccgaagt ggcgatgatc ttccaggacc
    12901 cgatgaccag ccttaacccg tgctacaccg tgggtttcca gatcatggaa gcgattaagg
    12961 tgcatcaggg cggtaataag agcactcgcc gtcagcgggc gattgacctg ctgaatcagg
    13021 tcggtattcc cgatccggca tcgcgtctgg atgtttaccc gcatcagctt tccggcggca
    13081 tgagccagcg cgtgatgatc gccatggcga ttgcctgtcg gccaaaactg ctgattgccg
    13141 atgagccgac cactgcgctg gacgtgacca ttcaggcgca aatcatcgaa ctgttgctgg
    13201 agctacagca gaaagagaac atggcgctgg tgttaattac ccatgatctg gcgctggtgg
    13261 cggaagctgc gcataaaatc attgtgatgt atgcaggcca ggtggtggaa acgggcgatg
    13321 cgcacgccat cttccatgcg ccgcgtcacc cgtatactca ggcattgctg cgtgcgctgc
    13381 cagaatttgc tcaggacaaa gaacgtctgg cgtcgttgcc aggtgtcgtt cccggcaagt
    13441 acgaccgccc gaacggctgc ctgcttaacc cgcgctgccc ctatgccact gacagatgtc
    13501 gcgctgaaga accggcgctg aatatgctcg ctgacgggcg ccagtccaaa tgccattacc
    13561 cacttgatga tgccgggagg ccgacactat gagtacgcaa gaggccaccc tgcaacaacc
    13621 gctgttgcag gctatcgacc tgaaaaaaca ttatccggtg aagaaaggca tgttcgcgcc
    13681 ggaacgtctg gttaaagcgc tggatggcgt ttcgtttaac cttgaacgtg gcaaaacgct
    13741 ggcagtagtg ggcgaatctg gctgcggtaa atcgaccctc ggtcggttgc tgacgatgat
    13801 tgaaacgccc accggtggcg agctgtatta ccaggggcag gatctgctta agcacgatcc
    13861 gcaggcgcag aagctgcgtc ggcagaaaat ccagatcgtc ttccagaacc cttacggttc
    13921 gctgaatccg cgtaaaaaag tcgggcaaat tcttgaagag ccgctgctga tcaacaccag
    13981 cttaagcaaa gaacagcgtc gggaaaaagc cctgtcgatg atggcgaaag tcggcctgaa
    14041 aaccgagcac tatgaccgct atccgcatat gttctccggc ggtcagcgtc agcgtatcgc
    14101 catcgcccgt ggtctgatgc tcgacccgga tgtggtgatt gccgatgaac cggtttccgc
    14161 gctggatgtt tcagtgcgcg cgcaggtgct gaatctgatg atggatttgc agcaggagtt
    14221 ggggctgtct tatgtcttta tctcccacga cctgtcggtg gtggagcaca ttgctgatga
    14281 agtgatggtg atgtacctgg gccgctgcgt ggagaaggga acgaaagacc aaatcttcaa
    14341 taacccgcgc catccgtaca ctcaggcgct actttccgcg acgccgcgcc tgaacccgga
    14401 cgatcgccgc gagcgcatca agctcagcgg tgaactacca agcccactga atccaccgcc
    14461 gggttgcgcc ttcaacgccc gctgtcgtcg gcgcttcggc ccctgcaccc agttgcagcc
    14521 gcagctaaaa gactacggcg gtcaactggt agcttgtttt gctgttgatc aggatgaaaa
    14581 tccgcagcgt taactcacaa aaccggggcc atgcgctccg gtttactcaa tcagcttcaa
    14641 cagtggcgac aagcatagca aaatgccgta cagcagaatc agccaggctt taaatccgcg
    14701 aagtttttcc agttgtgcga ctttatagat caggaaaaac ggaatgaggc acgacacaat
    14761 accatataac gggcttccca actgaaagaa caccagcacc gatacacgaa acgaaaccca
    14821 aatcgtcaac gtaatgacga taaaagcgca gatcgccaga gtcagcacgc gtgagttaat
    14881 tttcttggta tcaataattc ggcttaacag attgagaata atgcctttaa tggcctcgtg
    14941 gaaaccgaga taaatgccaa agaatgcggt cagtacggca aagatattaa gcaccgtaga
    15001 ggtgatatga atgatatgcc cagggatcac ctgcgcggcc agcgccagtg ctgagatatt
    15061 ttgttcaaag gcagaaacgg cttcttcgtg gctaattgag aaggtaaacg aaaaggcaaa
    15121 aaacaggatc accgcgatga gcgtgatata actaattcgg tgggtacgca gcgcgagccg
    15181 ggttgccagt actttatccg cttcccgttt acgataggca atattcattg ggttaagtac
    15241 ctgaataaat actgcagaaa agaagcaaaa tggaatggta agcaagacat cgcggaaaaa
    15301 gacggaggct tgcgggaagg cggttatatt ggcgaaattc cagtgcggga tcatcgcaaa
    15361 accgaacacg acaataatcc ctactttgac caccaccatt ggcccggaaa tcttaaacag
    15421 taatcgttca ccaccagacg caatcgctac cagtacggcg aaaatagcga ctttataaag
    15481 tagagattgt gaaagatcgg catcggttaa accgaaggtt ttcaggtacg aggcgctgtc
    15541 gaaaaccacg gagagagagt agataaaaat cccgtggata atcatcaaaa agtagataac
    15601 cccgaggaaa attccccagt tcttccccag gtaatgactg ataatatcgg tgtagtcatt
    15661 acaggaatcg ctttcagaaa gggtttttaa ataaatgtcc tgcactaccc aggtggcagg
    15721 ataagcaatg atcgctgcgg taataaatac ccaaattccc ttcaagccaa tttgtactgg
    15781 catcagcacg gttccggcac caatcgccat gcctatgcat aataaaaccc agccgaagtc
    15841 gtagcgtgta aacggcaatt tctgattgtg tttcgatagt gtgttgtgct gcataaagtc
    15901 ctgtccagaa agaagtcggc ctcgcaaaaa tgaataaacc cagtttctct tgcgcaacag
    15961 gtggagaagt cgaatacgca gtatgtaaaa aatgaggcaa cagattcact ggggaggtcg
    16021 cgaggaggcg caattctaca aaattgcgca tttcactacc agtctggagt cagcccggat
    16081 atgaactgca actattgcaa ataaagaaat taagctggaa ggcatcgcgc aatgaggaag
    16141 attgccgggt gctgacagta aaaagcaatc gtaaaatcag cggcaagccg aaagggttcc
    16201 ggtgaggcgc aatgttgcgg gggc
//
LOCUS       DAJTYZ010000090        15730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000090 DAJTYZ010000000
VERSION     DAJTYZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.090"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..290)
                     /locus_tag="OO596_002801"
                     /pseudo
     CDS             complement(1..290)
                     /locus_tag="OO596_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(524..1944)
                     /gene="espX1"
                     /locus_tag="OO596_002802"
                     /pseudo
     CDS             complement(524..1944)
                     /gene="espX1"
                     /locus_tag="OO596_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2429..2692)
                     /gene="rpsT"
                     /locus_tag="OO596_002803"
     CDS             complement(2429..2692)
                     /gene="rpsT"
                     /locus_tag="OO596_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6718614.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            2795..3013
                     /locus_tag="OO596_002804"
     CDS             2795..3013
                     /locus_tag="OO596_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6718615.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3021..3962
                     /gene="ribF"
                     /locus_tag="OO596_002805"
     CDS             3021..3962
                     /gene="ribF"
                     /locus_tag="OO596_002805"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6718616.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4005..6821
                     /gene="ileS"
                     /locus_tag="OO596_002806"
     CDS             4005..6821
                     /gene="ileS"
                     /locus_tag="OO596_002806"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6718617.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            6821..7315
                     /gene="lspA"
                     /locus_tag="OO596_002807"
     CDS             6821..7315
                     /gene="lspA"
                     /locus_tag="OO596_002807"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6718618.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7440..7889
                     /gene="fkpB"
                     /locus_tag="OO596_002808"
     CDS             7440..7889
                     /gene="fkpB"
                     /locus_tag="OO596_002808"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6718619.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            7891..8841
                     /gene="ispH"
                     /locus_tag="OO596_002809"
                     /gene_synonym="lytB"
     CDS             7891..8841
                     /gene="ispH"
                     /locus_tag="OO596_002809"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6718620.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            8907..9821
                     /gene="rihC"
                     /locus_tag="OO596_002810"
     CDS             8907..9821
                     /gene="rihC"
                     /locus_tag="OO596_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6718621.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            9988..10809
                     /gene="dapB"
                     /locus_tag="OO596_002811"
     CDS             9988..10809
                     /gene="dapB"
                     /locus_tag="OO596_002811"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6718622.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11265..12413
                     /gene="carA"
                     /locus_tag="OO596_002812"
     CDS             11265..12413
                     /gene="carA"
                     /locus_tag="OO596_002812"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6718623.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12431..15652
                     /gene="carB"
                     /locus_tag="OO596_002813"
     CDS             12431..15652
                     /gene="carB"
                     /locus_tag="OO596_002813"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6718624.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3812 a         3718 c         4196 g         4004 t
ORIGIN      
        1 ctctgatgat agtgtttatc attttcgtaa agatggtcgc tgaatgtcct gtaatcctgc
       61 gaagcataac gccaggcggc cacgctaaat cgagtggctg tttgtactag atatttatta
      121 taggcgacct gatagctcgt accttcgtgt ctggtatcat tatttaactt actactcgat
      181 cgggcagcgt aaaaagagat ggcacccagc ggtgtattcc aaccatttcc cagagttata
      241 gcattatagt tatcggataa aatcgtaccc gattatgtga tcaggttgtt attatttaga
      301 ttaatgttta aattttttaa tttcttgatg tgaagttgat gaaagtttga aaatttaata
      361 ttcttaattg ttcccttctt tataagaaga gcaaggtgcc atcattagag tgacagagta
      421 ggatgtttca gagcatgctt cagattgttt tttatttctc tgcagtgtat taataatcat
      481 cacagataat ttcattctaa aattacctct ttctttgagt gttttaatcc tgatgaacta
      541 tattttttat aaggagcatg cactcgttga tgctctgaag gtattttctc atttttgatg
      601 atgttttgtc gtaatcgtgc ataaacttat taagttcttt atacacatca ttatttagct
      661 ttacattatc gcgaactaaa gtttgtacca atggtatcaa aatttcgaaa cgggacggtt
      721 tgtcatcaat acttgccagg gttttgaata ctcctgtgta tctgtaacct aagtgcttat
      781 tgacaagttg agtggtccat cggccaggca taatatcttc acagcgcaaa ttttttatca
      841 tggtattagt gaacgatgtt ccaaatatgt tgcttttttt aatgaagagg ttgtttattt
      901 ctccatttga aaagatggtg tttgataagt cgctttcaaa acatgatata ttggttaata
      961 cacaacaaaa ataataccca tatgaaaggt tgctcttttc tattgttata tttgatagca
     1021 acattttatg tatattaata ttaagagcag tcaaatactg gatcttttga tcgttaagtt
     1081 catacatctc cagtgattta ttatagggta ataaaagtat aaattccttt atattatctc
     1141 cgcagtaaga aaagacgaca ttaaatttat tagtggccat gttttcagtt gagcagtaag
     1201 ctgatttttc tattaaccga ctgggcacgc ttttaaataa agataatttt ccacatagtt
     1261 ttgaatatgc cgaattgcta gttttaaagt tctgccacat tattccctgg ttatcatcta
     1321 tcgatgagga ggggatttta ttgcattgct cattgtatag ctcagggaaa atattttttt
     1381 taataagctt tttgatttcc tggttaagat gacttttttt cttgtctatt gcactgtatt
     1441 ttgcgttttg tgacgtttcc tgatgatcat aaaatgactg gagatttctg caggcctcat
     1501 aaaatatttc aggagtatat agctgtgggt atatttcttg gttgaagttt gcctttaaaa
     1561 aattaacgaa tttttcatgg cattcttggt atatcttaat ctcttcagaa agacattcgc
     1621 ttgttctgtc atcaaaaaca tccaatctaa gtaatgtgat acctttgtgt ttaaaaaata
     1681 aaattgaact gtatgttcta tgagtttaca tctataaaaa tatgcatctc tcagatgagt
     1741 atattgaaaa aaatgatgaa ggactggaga tggtttaggc aagttatggc ttctaaaaaa
     1801 gtcatcaaac aaaattaaga actctgtttt atactcgcaa aatactttta ctaactcatt
     1861 ttgtcttgtt ttatttatta ttacatcatc tactatattt gatttataaa gacaatgtgg
     1921 cgaagttgag ataccatttg tcattatcag tccttgaagt tgatgaattt ttattaattt
     1981 aatttatgag tgaatgtgac tactatcaaa aatagctcat taattttatg aagtagaata
     2041 taaataggta tttaacctgt gttgattgtt gttaattatt tacaagatct aataaggaga
     2101 tatttatgaa tggttaaagt gtataggaat ttaattcaaa aatagagtgg tcttaagttt
     2161 aatacctcaa tgtgtatcac aatatccaca ttctttgtag gggagtctgg agattgagta
     2221 gatattcttg ttcagaatgt atcagccgat ggttctacga ttcttaagcc acgaagagtt
     2281 cagaaagtgt aacggcatgt ctgcaaaagc cagcatcttt tgactatctg gcaaccggta
     2341 gtgtgttctc tcacgcatca taaaagcagc aggcataaaa aaaacccgct tgcgcgggct
     2401 ttttcacaaa gcttcagcaa attggcgatt aagccagttt gttgatctgt gcagtcaggt
     2461 tagccttatg acgtgcagct ttgtttttgt ggatcagacc tttagcagcc tgacggtcca
     2521 cgatcggttg catttcgtta aatgctttct gtgcagcagc tttgtcgcca gcttcgatag
     2581 ctgcgtatac tttcttgatg aaagtacgca tcatagagcg acggcttgcg ttgtgcttac
     2641 gagccttttc agactgaatg gcgcgcttct tagctgattt gatattagcc aaggtccaac
     2701 tcccaaatgt gttctatatg gacaattcaa aggccgagga atatgccctt ttagccttct
     2761 tttgtcaatg gatttgtgca aataagcgcc gttaatgtgc cggcactcgt tacgtagtga
     2821 tggcgcagga ttctaccagc ttgcggggtg tgaatacagc ttttccgcga taaaaattgc
     2881 agcaggcggt cactttcttc ccgtgatttg cgccatggca atgaaaagcc acttctttct
     2941 gatttcggta ctcaatcgcc ggttaacctt gaccgctgta caaggtatac tcggacgatt
     3001 ttcactgttt tgagccagac atgaagctga tacgcggcat acataatctc agccaggccc
     3061 cgcaagaagg gtgtgtgctg actattggta atttcgacgg cgtgcatcgc ggtcatcgcg
     3121 cgctgttaca gggcttgcag gaagaagggc gcaagcgcaa cttaccggtg atggtgatgc
     3181 tttttgaacc tcaaccactg gaactgtttg ctaccgataa agccccggca agactgaccc
     3241 ggctgcggga aaaactgcgt taccttgcag agtgtggcgt tgattacgtg ctgtgcgtgc
     3301 gtttcgacag gcgtttcgcg gcgttaaccg cgcaaaattt catcagcgat cttctggtga
     3361 agcatttgcg cgtaaaattt cttgccgtag gggatgattt ccgctttggc gctggtcgtg
     3421 aaggcgattt cttgttatta cagaaagcta gcatggaata cggcttcgat atcactagta
     3481 cgcaaacttt ttgcgaaggt ggcgtgcgta ttagcagcac cgctgtgcgt caggcgctgg
     3541 cggatgacaa tctggctctg gcagaaagtt tactggggca cccgtttgcc atctccgggc
     3601 gtgtagtcca cggtgatgaa ttagggcgca ctataggttt cccgacggcg aatgtaccgc
     3661 tacgccgtca ggtttccccg gtgaaagggg tttatgcggt agaagtgctg ggcctcggtg
     3721 aaaagccgtt acccggcgtg gcaaacatcg gaacacgccc aacggttgcc ggtattcgcc
     3781 agcagctgga agtgcatttg ttagatgttg caatggacct ttacggtcgc catatacaag
     3841 tagtgctgcg taaaaaaata cgcaatgagc agcgatttgc gtcgctggac gaactgaaag
     3901 cgcagattgc gcgtgatgaa ttaaccgccc gcgaattttt tgggctaaca aaaccggctt
     3961 aagcctgtta tgtaatcaaa ccgaaacacg gaaccgagaa tctgatgagt gactataaat
     4021 caaccctgaa tttgccggaa acagggttcc cgatgcgtgg cgatctcgcc aagcgcgaac
     4081 cgggaatgct ggcgcgttgg actgatgatg atctgtacgg catcatccgt gcggctaaaa
     4141 aaggcaaaaa aaccttcatt ctgcatgatg gccctcctta tgcgaatggc agcattcata
     4201 ttggtcactc ggttaacaag attctgaaag acattatcgt gaagtccaaa gggctttccg
     4261 gttatgactc gccgtatgtg cctggctggg actgccacgg tctgccgatc gagctgaaag
     4321 tagagcaaga atacggtaag ccgggtgaga aattcaccgc cgccgagttc cgcgccaagt
     4381 gccgcgaata cgcggcgacc caggttgacg gtcaacgcaa agactttatc cgtctgggcg
     4441 tgctgggcga ctggtcgcac ccgtacctga ccatggactt caaaactgaa gccaacatca
     4501 tccgcgcgct gggcaaaatc atcggcaacg gtcacctgca caaaggcgcg aagccagttc
     4561 actggtgcgt tgactgccgt tctgcgctgg cggaagcgga agttgagtat tacgacaaaa
     4621 cttctccgtc catcgacgtt gcttttcagg cggtcgatca ggatgcactg aaagcaaaat
     4681 ttgccgtaag caacgttaac ggcccaatct cgctggtgat ctggaccact acgccgtgga
     4741 ctctgcctgc gaaccgcgca atctctattg cacctgattt cgactatgcg ctggtgcaga
     4801 tcgacggtca ggccgtgatt ctggcgaaag atctggttga aagcgtaatg cagcgtatcg
     4861 gcgtgaccga ttacaccatt ctcggcacgg taaaaggtgc ggagcttgag ttgctgcgct
     4921 ttacccatcc gtttatgggc ttcgacgttc cggcaatcct cggcgatcac gttaccctag
     4981 atgccggtac cggtgccgtt cacaccgcgc ctggccacgg cccggacgac tatgtgatcg
     5041 gtcagaaata cggcctggaa accgctaacc cggttggccc ggacggcact tatctgccgg
     5101 gcacttatcc gacgctggat ggcgtgaacg tcttcaaagc gaacgacatc gtcgttgcgc
     5161 tgctgcagga aaaaggcgcg ctgctgcacg ttgagaaaat gcagcacagc tatccgtgct
     5221 gctggcgtca caaaacgccg atcatcttcc gcgcgacgcc gcagtggttc gtcagcatgg
     5281 atcagaaagg tctgcgtgcg cagtcactga aagagatcaa aggcgtgcag tggatcccgg
     5341 actggggcca ggcgcgtatc gagtcgatgg ttgctaaccg tcctgactgg tgtatctccc
     5401 gtcagcgcac ctggggcgta ccgatgtcac tgttcgtgca caaagacacg gaagagctgc
     5461 atccgcgtac tctcgaacta atggaagaag tggcaaaacg cgttgaagtt gacggcatcc
     5521 aggcgtggtg ggatcttgat gcgaaagaga tcctcggcga tgaagctgat cagtacgtga
     5581 aagtgccgga cacattggat gtatggtttg actccggatc tacccactct tctgttgttg
     5641 acgtgcgtcc ggaatttgcc ggtcacgcag cggacatgta tctggaaggt tctgaccagc
     5701 accgtggttg gttcatgtct tccctaatga tctccaccgc gatgaaaggc aaagcgccgt
     5761 atcgtcaggt actgacccac ggctttaccg tggatggtca gggtcgcaag atgtctaaat
     5821 ccatcggcaa taccgtttcg ccgcaggatg tgatgaacaa actgggcgcg gatattctgc
     5881 gtctgtgggt ggcatcaacc gactacaccg gtgaaatggc cgtttctgac gagatcctga
     5941 aacgtgctgc cgatagctat cgtcgtatcc gtaacaccgc gcgcttcctg ctggcaaacc
     6001 tgaacggttt tgatccagca aaagatatgg tgaaaccgga agagatggtg gtactggatc
     6061 gctgggccgt aggttgtgcg aaagcggcac aggaagacat cctcaaggcg tacgaagcat
     6121 acgatttcca cgaagtggta cagcgtctga tgcgcttctg ctccgttgag atgggttcct
     6181 tctacctcga catcatcaaa gaccgtcagt acaccgccaa agcggacagt gtggcgcgtc
     6241 gtagctgcca gactgcgctg tatcacatcg cagaagcgct ggtgcgctgg atggcaccaa
     6301 tcctctcctt caccgctgat gaagtgtggg gctacctgcc gggcgaacgt gaaaaatacg
     6361 tcttcaccgg tgagtggtac gaaggcctgt ttggcctggc agacagtgaa gcgatgaacg
     6421 atgcgttctg ggacgagctg ttgaaagtgc gtggcgaagt gaacaaagtc attgagcaag
     6481 cgcgtgccga caagaaagtg ggtggctcgc tggaagcggc agtaaccttg tatgcagaac
     6541 cggaactgtc ggcgaaactg accgcgctgg gcgatgaatt acgatttgtc ctgttgacct
     6601 ccggcgctac cgttgcagac tataacgacg cacctgctga tgctcagcag agcgaagtac
     6661 tcaaagggct gaaagtcgcg ttgagtaaag ccgaaggtga gaagtgccca cgctgctggc
     6721 actacaccca ggatgtcggc aaggtggcgg aacacgcaga aatctgcggc cgctgtgtca
     6781 gcaacgtcgc cggtgacggt gaaaaacgta agtttgcctg atgagtcaat cgatctgttc
     6841 aacagggcta cgctggctgt ggctggtggt agtcgtgctg attatcgatc tgggcagcaa
     6901 atacctgatc ctccagaact ttgctctggg ggatacggtc ccgctgttcc cgtcgcttaa
     6961 tctgcattat gcgcgtaact atggcgcggc gtttagtttc cttgccgata gcggcggctg
     7021 gcagcgttgg ttctttgccg gtattgcgat tggtattagc gtgatcctgg cagtgatgat
     7081 gtatcgctcg aaggccacgc agaagctaaa caatatcgct tacgcgctga ttattggcgg
     7141 cgcgctgggc aacctgttcg accgcctgtg gcacggcttc gttgtcgata tgatcgactt
     7201 ctacgtcggc gactggcact tcgccacctt caaccttgcc gatactgcca tctgtgtcgg
     7261 tgcggcactg attgtgctgg aaggtttttt gccttctaga gcgaaaaaac aataataaac
     7321 cctgccggat gcgatgctga cgcatcttat ccggcctaca gattgctgcg aaatcgtagg
     7381 ccggataagg cgtttacgcc gcatccggca aaaatcctca aatataagag caaacctgca
     7441 tgtctgaatc tgtacagagc aatagcgccg tcctggtgca cttcacgcta aaactcgacg
     7501 atggcaccac cgccgagtct acccgcaaca acggtaaacc ggcgctgttc cgcctgggtg
     7561 atgcttctct ttctgaaggg ctggagcaac acctgttggg gctgaaagtg ggcgataaaa
     7621 ccaccttctc gttggagcca gatgcggcgt ttggcgtgcc gtcaccggac ctgattcagt
     7681 acttttcccg ccgtgaattt atggatgcag gcgagccaga aattggcgca atcatgcttt
     7741 ttaccgcaat ggatggcagt gagatgcctg gcgtgatccg cgaaattaac ggcgactcca
     7801 ttaccgttga tttcaaccat ccgctggccg ggcagaccgt tcattttgat attgaagtgc
     7861 tggaaatcga tccggcattg gaggcgtaac atgcagatcc tgttggccaa cccgcgtggt
     7921 ttttgtgccg gggtagaccg cgctatcagc attgttgaaa acgcgctggc catttacggc
     7981 gcaccgatat atgtccgtca cgaagtggta cataaccgct atgtggtcga tagcttgcgt
     8041 gagcgtgggg ctatctttat tgagcagatt agcgaagtac cggacggcgc gatcctgatt
     8101 ttctccgcac acggtgtttc tcaggcggta cgtaacgaag caaaaagccg cgatttgacg
     8161 gtgtttgatg ccacctgtcc gctggtgacc aaagtgcata tggaagtcgc ccgcgccagc
     8221 cgtcgtggcg aagagtcgat tctcatcggc cacgccgggc atccggaagt ggaaggtaca
     8281 atgggccagt acagtaaccc ggaaggggga atgtatctgg tcgaatcgcc ggacgatgtg
     8341 tggaaactga cggtcaaaaa cgaagagaag ctctccttta tgacccagac cacgctgtcg
     8401 gtggatgaca cgtctgatgt gatcgacgcg ctgcgtaaac gcttcccgaa aattgtcggt
     8461 ccgcgcaaag atgacatctg ctacgccacg actaaccgtc aggaagcggt acgcgccctg
     8521 gcagaacagg cggaagttgt gttggtggtc ggttcgaaaa actcctccaa ctccaaccgt
     8581 ctggcggagc tggcccagcg tatgggcaaa cgcgcgtttt tgattgacga tgcgaaagat
     8641 atccaggaag agtgggtgaa agaggttaaa tgcgtcggcg tgactgcggg cgcatcggct
     8701 ccggatattc tggtgcaaaa tgtggtggca cgtttgcagc agctgggcgg tggtgaagcc
     8761 attccgctgg aaggccgtga agaaaacatt gttttcgaag tgccgaaaga gctgcgtgtc
     8821 gatattcgtg aagtcgatta agtcattagc agcctaagtt atgcgaaaat gccggtcttg
     8881 ttaccggcat tttttatgga gaaaacatgc gtttacctat cttcctcgat actgaccccg
     8941 gcattgacga tgccgtcgcc attgccgccg cgatttttgc ccccgaactc gacctgcaac
     9001 tgatgaccac cgtcgcgggt aatgtctcgg ttgagaaaac cacccgcaac gccctgcaac
     9061 tgctgcattt ctggaatgcg gagattccgc tcgcccaggg agccgctgtg ccgctggtac
     9121 gcgcaccgcg tgatgcagca tctgtgcacg gcgaatcggg aatggctggc tacgactttg
     9181 ttgagcacaa ccgaaagcca atcgggatac cagcgtttct ggcgattcgt gatgccctga
     9241 tgcgtgcacc agagcctgtt accctggtgg ccattggccc gttaaccaat attgcgctgt
     9301 tactttcaca atgcccggaa tgtaaaccgt atattcgtcg tctggtgatc atgggcggtt
     9361 cttctggtcg cggcaactgt acaccaaatg ccgagtttaa tatcgctgcc gatccagaag
     9421 cggctgcctg tgtcttccgt agcggaatcg aaatcgtcat gtgcggtctg gatgtcacca
     9481 atcaggcaat attaacccct gactatctcg ctactttgcc ggagttgaat cgcaccggaa
     9541 aaatgcttca tgcactgttt agccactacc gtagcggcag tatgcaaagc ggtctgcgaa
     9601 tgcacgatct ctgcgccatc gcctggctgg tgcgcccgga cttattcacc ctcaagccgt
     9661 gttttgtcgc ggtagaaact caaggcgaat ttacctcagg cacgacggtg gttgatatcg
     9721 acggttgcct gggcaaacca gccaatgtaa aggtggcatt ggatctggat gtgaaaggct
     9781 tccagcagtg ggtggctgag gtgctggctc tggcgtcgta acctgtcaca tgttatcggc
     9841 atgcagtcat tcatcgactc atgcctttca ctgatatccc tccctgttta tcattaattt
     9901 ctaattatca gcgtttttgg ctggcggcgt agcgatgcgc tggttactct gaaaacggtc
     9961 tatgcaaatt aacaaaagag aatagctatg catgatgcaa acatccgcgt tgccatcgcg
    10021 ggagccgggg ggcgtatggg ccgccagttg attcaggcgg cgctggcatt agaaggcgtg
    10081 cagctgggcg cggcgctgga gcgcgaagga tcttctttac tgggcagcga cgccggtgag
    10141 ctggctggtg cagggaaaac aggcgttacc gtgcaaagca gcctcgatgc ggtaaaagat
    10201 gattttgatg tgtttatcga ttttacccgt ccggaaggta cgctgaacca tctcgctttt
    10261 tgtcgccagc atggcaaagg gatggtgatc ggcactacgg ggtttgacga agccggtaaa
    10321 caggcgattc gtgacgccgc cgccgatatt gcgattgtct ttgccgccaa ttttagcgtt
    10381 ggcgttaacg tcatgcttaa gctgctggag aaagcagcca aagtgatggg tgactacacc
    10441 gatatcgaaa ttattgaagc acatcataga cataaagttg atgcgccgtc aggcaccgca
    10501 ctggcaatgg gagaggcgat cgcccacgcc cttgataaag atctgaaaga ttgcgcggtc
    10561 tacagtcgtg aaggccacac cggtgaacgt gtgcctggca ccattggttt tgccaccgtg
    10621 cgtgcaggtg acatcgttgg tgaacatacc gcgatgtttg ccgatattgg cgagcgtctg
    10681 gagatcaccc ataaggcgtc cagccgtatg acatttgcta acggcgcggt aagatcggct
    10741 ttgtggttga gtggtaagga aaatggtctt tttgatatgc gagatgtact taatctcaat
    10801 aatttgtaac cacaaaatat ttgttatggt gcaaaaataa cacatttaat ttattgatta
    10861 taaagggctt taatttttgg cccttttatt tttagtgtta tgtttttaaa tattctataa
    10921 gtgccaaaaa ttacatgttt tgtcttctat ttttgttgtt ttaatgtaaa ttttgaccat
    10981 ttggtccact ttttcctgct cgtttttatt tcatgcaatc ttcttgctgc gcaagcgttt
    11041 tccagaacag gttatatgat ctttttgtcg cttaatgcct gtaaaatatg catgagccac
    11101 aaaataatat aaaaaatacc gccattaagt tgacttttag cgcccatatc tccagaatgc
    11161 cgccgtttgc cagaaattcg tcggtaagca gatttgcatt gatttacgtc atcattgtga
    11221 attaatatgc aaataaagtg agtgaatatt ctctggaggg tgttttgatt aagtcagcgc
    11281 tattggttct ggaagacgga acccagtttc acggtcgggc cataggggca acaggttcgg
    11341 cggttgggga agtcgttttc aatacttcaa tgaccggtta tcaagaaatc ctcactgatc
    11401 cttcctattc tcgtcaaatc gttactctta cttatcccca tattggcaat gtcggcacta
    11461 atgacgccga tgaagaatct tctcaggtac atgcacaagg tctggtgatt cgcgatctgc
    11521 cgctgattgc cagcaacttc cgtaataccg aagacctctc ttcttacctg aagcgccata
    11581 acatcgtggc gattgccgat atcgataccc gtaagctgac gcgtttactg cgcgagaaag
    11641 gcgcacagaa tggctgcatt atcgcaggcg ataacccgga tgcggcgttg gcgttagaaa
    11701 aagcccgcgc gttcccaggt ctgaatggca tggatctggc aaaagaagtg accaccgcag
    11761 aagcctatag ctggacacaa gggagctgga cgttgaccgg cggcctgcca gaagcgaaaa
    11821 aagaagacga gctgccgttc cacgtcgtgg cttatgattt tggtgccaag cgcaacatcc
    11881 tgcggatgct ggtggataga ggctgtcgcc tgaccatcgt tccggcgcaa acttctgcgg
    11941 aagatgtgct gaaaatgaat ccagacggca tcttcctctc caacggtcct ggcgacccgg
    12001 ccccgtgcga ttacgccatt accgccatcc agaaattcct cgaaaccgat attccggtat
    12061 tcggcatctg cctcggtcat cagctgctgg cgctggcgag cggtgcgaag actgtcaaaa
    12121 tgaaatttgg tcaccacggc ggcaaccatc cggttaaaga tgtggagaaa aacgtggtga
    12181 tgatcaccgc ccagaaccac ggttttgcgg tggatgaagc aacattacct gcaaacctgc
    12241 gtgtcacgca taaatccctg ttcgacggta cgttacaggg cattcatcgc actgataaac
    12301 cggcgttcag cttccagggt cacccggaag ccagccctgg gccacacgac gcagcgccgt
    12361 tgttcgacca ctttatcgag ttaattgagc agtaccgtaa aaccgctaag taatcaggag
    12421 taaaagagcc atgccaaaac gtacagatat aaaaagtatc ctgattctgg gtgcgggccc
    12481 gattgttatc ggtcaggcgt gtgagtttga ctactctggc gcgcaagcat gtaaagccct
    12541 gcgcgaagag ggttaccgcg tcattctggt gaactccaac ccggcgacca tcatgaccga
    12601 cccggaaatg gccgatgcga cctacatcga gccgattcac tgggaagtag tacgcaagat
    12661 tattgaaaaa gagcgcccgg acgcggtgct gccaacgatg ggcggtcaga cggcgctgaa
    12721 ctgcgcgctg gagctggagc gtcagggcgt gttggaagag ttcggcgtga ctatgattgg
    12781 tgcgaccgcc gatgcgattg ataaagcaga agaccgccgt cgtttcgacg tagcgatgaa
    12841 gaaaattggt ctggaaaccg cgcgttccgg tatcgcacac acgatggaag aagcgctggc
    12901 ggttgccgct gacgtgggct tcccgtgcat tattcgccca tcctttacca tgggcggtag
    12961 cggcggcggt atcgcttata accgcgaaga gtttgaagaa atttgcgccc gcggtctgga
    13021 tctctcccca accaaagagc tgctgattga tgagtcgctg atcggctgga aagagtacga
    13081 gatggaagtg gtgcgtgata aaaacgacaa ctgcatcatc gtctgctcta tcgaaaactt
    13141 tgatgcgatg ggcatccata ccggtgactc catcactgtc gcgccagccc aaacgctgac
    13201 cgacaaagaa tatcaaatca tgcgtaacgc ctcgatggcg gtgctgcgtg aaatcggcgt
    13261 tgaaaccggt ggttccaatg tccagtttgc ggtgaacccg aaaaacggtc gcctgattgt
    13321 tatcgaaatg aacccacgcg tgtcccgttc ttcggcgctg gcgtcgaaag cgaccggttt
    13381 cccgattgct aaagtggcgg cgaaactggc ggtgggttac accctcgacg aactgatgaa
    13441 cgacatcact ggcggacgta ctccggcctc cttcgagccg tccatcgact atgtggttac
    13501 taaaattcct cgcttcaact tcgaaaaatt cgctggtgct aacgaccgtc tgaccactca
    13561 gatgaaatcg gttggcgaag tgatggcgat tggtcgcacg cagcaggaat ccctgcaaaa
    13621 agcgctgcgc ggcctggaag tcggtgcgac tggattcgac ccgaaagtga gcctcgatga
    13681 cccggaagcg ttaaccaaaa tccgtcgcga actgaaagat gctggcgcag agcgtatctg
    13741 gtacatcgcc gatgccttcc gtgcgggcct gtctgtggac ggcgtgttca acctgaccaa
    13801 tattgaccgc tggttcctgg tacagattga agaactggtg cgtctggaag agaaagtagc
    13861 ggaagtgggc atcactggcc tgaacgctga attcctgcgc cagctgaaac gcaaaggctt
    13921 tgccgatgcg cgtctggcaa aactggcggg cgtgcgcgaa gcggaaatcc gcaagctgcg
    13981 cgaccagtat gacctgcacc cggtttataa gcgcgtggat acctgtgcgg cagagttcgc
    14041 caccgacacc gcttacatgt actccactta tgaagaagag tgcgaagcga atccgtctac
    14101 cgaccgtgaa aaaatcatgg ttctcggcgg cggcccgaac cgtatcggtc agggtatcga
    14161 attcgactac tgctgtgtac acgcctcgct ggcgctgcgc gaagacggtt acgaaaccat
    14221 tatggttaac tgtaacccgg aaaccgtctc caccgactac gacacttccg atcgcctcta
    14281 cttcgagccg gtaactctgg aagatgtgct ggaaatcgtg cgtattgaga agccgaaagg
    14341 cgttatcgtt cagtacggcg gtcagacccc gctgaaactg gcgcgcgcgc tggaagctgc
    14401 tggcgtaccg gttatcggca ccagcccgga tgctatcgac cgtgcagaag accgtgaacg
    14461 cttccagcat gcggttgagc gtctgaaact gaaacaaccg gcgaacgcca ccgttaccgc
    14521 tattgaaatg gcggtagaga aggcgaaaga gattggctac ccactggtgg tacgtccgtc
    14581 ttacgttctc ggcggtcggg cgatggaaat cgtctatgac gaagctgacc tgcgtcgcta
    14641 cttccagacg gcggtcagcg tgtctaacga tgcgccagtg ttgctggatc acttccttga
    14701 tgacgcggta gaagttgacg tggatgccat ctgcgacggc gaaatggtgc tgattggcgg
    14761 catcatggag cacatcgagc aggcgggcgt gcactccggt gactccgcat gttctctgcc
    14821 agcctacacc ttaagtcagg aaattcagga tgtgatgcgc cagcaggtgc agaaactggc
    14881 cttcgaattg caggtgcgcg gtctgatgaa cgtgcagttt gcggtgaaaa acaacgaagt
    14941 ctacctgatt gaagttaacc cgcgtgcggc gcgtaccgtt ccgttcgtct ccaaagccac
    15001 cggcgtaccg ctggcaaaag tggcggcgcg tgtgatggct ggcaaatcgc tggctgagca
    15061 gggcgtaacc aaagaagtta tcccgccgta ctactcggtg aaagaagtgg tgctgccgtt
    15121 caataaattc ccgggtgttg acccgctgtt agggccagaa atgcgctcca ccggggaagt
    15181 gatgggcgtg ggccgcacct tcgctgaagc gtttgccaaa gcgcagctgg gcagcaactc
    15241 caccatgaag aaacacggtc gtgcgttgct ttccgtgcgc gaaggcgata aagaacgcgt
    15301 ggtggacctg gcggcaaaac tgctgaaaca gggcttcgag ctggatgcga cccacggcac
    15361 ggcgattgtg ctgggcgaag cgggcattaa tccgcgtctg gtgaacaagg tgcatgaagg
    15421 ccgtccgcac attcaggacc gcatcaagaa tggcgaatat acctacatca tcaacaccac
    15481 ttcaggccgt cgcgcgattg aagactcccg cgtgatccgt cgcagtgcgc tgcaatataa
    15541 agtgcattat gacaccaccc tgaacggtgg tttcgctacc gcgatggcgc tgaatgccga
    15601 cgcgaccgaa aaagtaattt cggtgcagga aatgcacgcg cagatcaaat aatagcgtgt
    15661 catggcggat atttttcatc cgctaatttg atcgaataag taatacggtt ctctgatgag
    15721 gaccgttttt
//
LOCUS       DAJTYZ010000091        15563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000091 DAJTYZ010000000
VERSION     DAJTYZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.091"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..192)
                     /locus_tag="OO596_002814"
     CDS             complement(<1..192)
                     /locus_tag="OO596_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="periplasmic protein induced by stress response via
                     Cpx and BaeSR system; similar to CpxP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6718625.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQ"
     gene            complement(522..1490)
                     /gene="astE"
                     /locus_tag="OO596_002815"
     CDS             complement(522..1490)
                     /gene="astE"
                     /locus_tag="OO596_002815"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6718626.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1483..2826)
                     /gene="astB"
                     /locus_tag="OO596_002816"
     CDS             complement(1483..2826)
                     /gene="astB"
                     /locus_tag="OO596_002816"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6718627.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(2823..4301)
                     /gene="astD"
                     /locus_tag="OO596_002817"
                     /pseudo
     CDS             complement(2823..4301)
                     /gene="astD"
                     /locus_tag="OO596_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4298..5332)
                     /gene="astA"
                     /locus_tag="OO596_002818"
     CDS             complement(4298..5332)
                     /gene="astA"
                     /locus_tag="OO596_002818"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6718628.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5329..6549)
                     /gene="astC"
                     /locus_tag="OO596_002819"
     CDS             complement(5329..6549)
                     /gene="astC"
                     /locus_tag="OO596_002819"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR6718629.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            6995..7801
                     /gene="xthA"
                     /locus_tag="OO596_002820"
     CDS             6995..7801
                     /gene="xthA"
                     /locus_tag="OO596_002820"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6718630.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            7968..8678
                     /locus_tag="OO596_002821"
     CDS             7968..8678
                     /locus_tag="OO596_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6718631.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8683..9360
                     /gene="ydjY"
                     /locus_tag="OO596_002822"
     CDS             8683..9360
                     /gene="ydjY"
                     /locus_tag="OO596_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6718632.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9375..10082
                     /locus_tag="OO596_002823"
     CDS             9375..10082
                     /locus_tag="OO596_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6718633.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10082..10630
                     /locus_tag="OO596_002824"
     CDS             10082..10630
                     /locus_tag="OO596_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6718634.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10640..11806
                     /locus_tag="OO596_002825"
     CDS             10640..11806
                     /locus_tag="OO596_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6718635.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            11779..13315
                     /locus_tag="OO596_002826"
                     /pseudo
     CDS             11779..13315
                     /locus_tag="OO596_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13315..13968
                     /locus_tag="OO596_002827"
     CDS             13315..13968
                     /locus_tag="OO596_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6718636.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14035..15342
                     /gene="ynjE"
                     /locus_tag="OO596_002828"
     CDS             14035..15342
                     /gene="ynjE"
                     /locus_tag="OO596_002828"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR6718637.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15351..>15563)
                     /locus_tag="OO596_002829"
     CDS             complement(15351..>15563)
                     /locus_tag="OO596_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6718638.1"
                     /translation="QIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWI
                     FGALCWMTTFTRVWSGYLTLKSLQRQ"
BASE COUNT         3617 a         4244 c         4063 g         3639 t
ORIGIN      
        1 ctgctgtttc tgcgcgtcgg tcaggttcag gtctttgaac atcatgtcct gatgcggacc
       61 gaacttgcct ttgtggtgca tcatcggctt cgcgtcagcc ggtgctgcgg tagtggtgtc
      121 tgcggcatgg gccaggttag ccgcgccaag agccagggta gaggcaacaa acagtgcagt
      181 taatttacgc atattctata tccttccttt cagttattta ttacggcttt cttaagtagc
      241 gtgccgtgtt gacgagatta actttactga ctttagcgtc aattaatcag agcaacggta
      301 aaacaatgaa agtgtaaaaa acacttttgc gccaattatg gagaaaaaaa gaaaatttga
      361 tggagagtga tgagagaata ttacaacaca atgattttgc agagattatg aagaactata
      421 ccggatgact ggtgataaat aaagcaaata accaggatta atctgtatta atttataaga
      481 aagcaactta atacccgcag aatgatttct gcgggtaagt attagcttat tttttcgagc
      541 attaatcccg cgcgtaatcc caacgctacc aacggattag ggaataacac atactctaca
      601 tcatgggtta cggtaaaacg ttcctctccg tcctgcgcca gcaatgttcc tttctcaaac
      661 ggcataaaat tcagcgtgtc acttgccata tgcatttcga aggacggcga gtggcgagta
      721 atttgcgaaa ccacccgata acggagcggc ggtgttctca cgataccgac actctcacca
      781 gatagcagcg cagcaattgc gctggcagtt actgcaaact ggcgaagatc gttttgccca
      841 aagggcaacg ctttgccaag ttccagcgta caggccagcg cgccaaaatg tctggcgctg
      901 aaatgggtaa acgtaccacc aggctcctga tggaacacca gcgcctccag ccccgccgca
      961 cccagccacg tcagaaattt ctcgtcccag ggaatgtcgc gttgcggtaa tacaccgaac
     1021 tgaggatgca aggagccacg aattgcggta tgtagatcaa ggtgccaacg cacagattct
     1081 ttgccctggt cataaaaatc ttccaggcac tgttccagtt cgcgcgcccg acaggtttct
     1141 ccgctttcag caaatagctg ccaacgaccg ccaaacattc ggttcatatc gctatggcaa
     1201 taacgtttcc cttgcttcag cgcaggagga tccccgagga tcaccagcaa ccgccaacgt
     1261 aacgggatct cgccgtgaga tatcgcgcca agcaacgcgt ccagcatttc cacaggtgcc
     1321 gtctcattac cgtggatccc cgcagaaatc accagcgcgc cttgcggtgg cgttaatggc
     1381 gtcagttcca gcacaccatc gcccagccag cgccagcgaa cgccgttgat ttctcgctcg
     1441 gtgataaccg gttttttacc cgttaaggtc agagcaagaa aattatccat tgccccctcc
     1501 ctcgcgctgg aacggataaa ccgaaccgag attcagtaat tggctcaata catccagtgc
     1561 ttcccgccct tcgcgcagca attgcgggtc ggccagatcg gcagcagtaa ggcgatcgcg
     1621 gtagtaacga tccacccagt cattgagcgc attaaacagc gtatcgttca tcatcaccgc
     1681 cggattcacc gcccggcgtt cttcttctgt caatactacc cgcaaccgca ggcacgccgg
     1741 gccgccgcca ttcgccatgc tttcacgtaa atcaaagact tttagttcgc taatcgggtt
     1801 gtcagcggca aggagttcat tgagataacc ccatactccg gcgtgttccc gacactcctg
     1861 aggcagcacc aacatcatgg aaccatcatc gcggctcaac agttggctgt taaacagata
     1921 ggtagacacc gcatccgaca cggaaacctg agttgccgga acttctatcg ccataaaacc
     1981 gttcacccgc gcccgcaggt ttgccagtaa ctgtgactgg cgagcgaacg cctgttggtg
     2041 gcaaaacagc acctggcggt tacttacggc aatcacgtca ttatgaaaaa caccctggtc
     2101 gataacgtcc gggttttgct gggcgaaaat cacctgttgg ggattcacct gattaagcct
     2161 tgccaccgcc tcgctggctt cgcgagtctg tcgcgccgga taacgggaag gccgggtatc
     2221 attgccttct tctcgcccgt agacaaaaag ttgcataccc ggctcaccgt aatgaccgcc
     2281 gagacgattg tggtttgccg ccccctcatc accgagcaac gctacctgcg gcaacgccga
     2341 atggacgcta aatttctctt cgtcgttaaa aatagctttt aacagcgatt cagtgacggg
     2401 cgcttccagc gaacggtgaa atttattgtt caggttggca acggtgagat gcactttacc
     2461 atccagcgta tcggcagatg gcgcgatcgt tgccgcattg gctacccaca ttggcgaagc
     2521 ggagctgacg ctggaaagcc agtgcggtgc ctggcgtgca actttttcca gtacctgctc
     2581 atcgctacca ctgaatccca actgacgcag caccggaata aacggacgct cgtgcggcgg
     2641 gatcacggcc tgggggaatc ccgcatcggc aagggttttc attttcagta agccctgttt
     2701 cgccgccagt cgcgggttag acacctgaaa acggtgacgg gtagaggctt cattaccaaa
     2761 cgacaggcca gcgtaatgat gcgtcagccc taccagcccg tcgaaattaa cttcccaggc
     2821 gttcatcgca ccacctcatc ggaaaaatcc agcccggggt taagcgttgc gggcaaagtt
     2881 aacgagtccg actccaggct cgccataggc catgcgcagt aatctgcggc ataccaggcg
     2941 ctggggcgat ggttaccgga tgcaccaatg ccgccgaatg gcgcggtact ggcagcacca
     3001 gtaagcggtt tgttccagtt aacaatcccc acccgcgcct ccagcaacag ttgatcgaat
     3061 ttttcccgct cgggggaaac cagaccgcaa gagagtccga agcgagtgtt attcgccatt
     3121 agaatcgctt cctcgaaaga atcataacgc cagacgcgca ataacggtcc gaacacctct
     3181 tcatctggta cgccagcaac gcctgtcatt tcaatgatcc ccggcgtcag caacgatgtc
     3241 tctgattgta ataagcgcgg cgcaagcagg gttcgtccgc ccatcgcttc cagttgctgc
     3301 taggcagtaa ccacctgctg tgcggcctgt tcagaaatca gcccgccaat aaacggctgc
     3361 ggttcgtcat cccagttgcc cggcgttaat cgctggctga cggcaaccag acgagcaaga
     3421 aacgcatcgc cctgtgctcc gcttttgagc aataaacggc gggcgcaggt gcagcgttgc
     3481 ccggctgtga caaacgccga ctgaatggtc agatggacag ccgagtcgat atccgccacc
     3541 tcatcgataa ttagcggatt attaccgccc atctcaaggg cgagaatttt ctccggctga
     3601 ccggagagct ggcgatgcag ctggtagccg gtattggcgc taccggtaaa cagcaaaccg
     3661 tcgagatcct ccagcgcact cagcgcctga cccgtttcac gcccgccctg caccaggttc
     3721 agcacgcccg gcggcaagcc agcctgctgc cataaacgca ttaccgcttc gccactccac
     3781 ggtgtcagtt cgctgggttt aaagataatg gtgttacctg ccagcaatgc cggaacgata
     3841 tgtccgttcg gcaaatgacc agggaaatta tacggcccaa acaccgccag cacgccgtgc
     3901 gggcgatgtc gcaggctcgc cgcaccgtcc ggcatttcac tacgctgctc gccggtacga
     3961 acgtgatacg ccttaattga tatcgcgatt ttattgatca tcgccgtcac ttcggttgcc
     4021 gcttcccagc gcggcttacc cgtttctctg gcaataatcg cggttaattc ggctttattg
     4081 ctttccagca atccggcaaa gcgttcgaca acgacctgac gttcagccaa tgagagccgc
     4141 gcccagcgcg gaaacgccgc acgggctgcc cgacaagcct gttcgacctg agcggcatcg
     4201 gcatcattgc cttgccataa cacctcgccc gataccggat tacgcttcac acgcgatgcg
     4261 ccctggcccg ttatccagtc accgttaatc cataaagtca tgctgttttc tcctctgcgc
     4321 acaggcgcac cagacgaacg cgatcccctg cgtggcattt gagggcatcc agttgtgcgg
     4381 cggttaaaat caaacgctcg gttgccggat cggtacgcgc cagcaccacg cggaaatggt
     4441 gataattttc attggcgacc aggcaggccg gaaaatcgcc ctgcgcaggc tgcccttctg
     4501 ccacttccac cagccgactt ttacggatgg cgcgcacgcg gtcgatgtca cactcaagcg
     4561 ttggcccgcc gtcgaagatg tcgatatagt tgcggtaacg aaaaccttct ttctccagca
     4621 ccgcgcgggc aggcgcggtt tgcggatgta cctgaccgat gacgtcctgg gcttcctggg
     4681 ataaaaagtg ggtatagatc ggatgtttcg gcatcagttc tgcaataaat gccttttgcc
     4741 cggtgccgca gagaaaatcg acgcggctaa aatccatcga aaagaagcgt ttaccgaggc
     4801 tttgccagaa cggtgaatag ccgtgttcgt caatcacccc gcgcatttca gcaaccactt
     4861 tgtcattaaa cttgtcgcga aaagccgcca taaacataaa gcgcgatttc gacagcaaat
     4921 agccgttgcc ctctttgcgc cagtccgggt cgagaaacag cgtgcacagc tcgctgctgc
     4981 cggtgtgatc gttactaaga aacagcgtcg gcaatgcgtt atagacattc agctcttttg
     5041 aggcgtgaac caacgtgccg acccgatagt tgtaccaggg atcgttcagc ccaaccgcca
     5101 cctcaatggc gcaaatcccc gccacggtgc ctgtctcgct atcttccagc acgaacacat
     5161 agccctgctc gcttttgggc agttcgcctt gccaggtttt gattgccctt tcgatacgcg
     5221 cggaaagcgt ggcttcattg gcgggaagcg acgtcaggcc gccgcccgtt ttgctggcaa
     5281 gctgcatcag cgccgagaca tctgagcgct caacgggacg gatgaccatc atgatgaacc
     5341 tcggctaaca aagtgttcgc aagcagctgc aaagcgatcc agtccggtcg tcacctcttc
     5401 ttcgctgaca ttgagcgcag gcgcaaaacg caccacgttg ccacccgcaa tcagtaccat
     5461 aacgcctgct ttcgccgcct cctgagagat ctgtttcgct tgtccggcgt aatcggcatt
     5521 cagtacacag ccaatcaaca aacctaagcc gcgaacttca ctgaacaaac cgtagcggtg
     5581 attaatggta ttaagacgct caacaaacca gtcgtgacgc tgtttaacgc cattaagcat
     5641 ctctggtgtg ttgatgagct ccagcacttt gcccgccacc gccgaggcca gcggattgcc
     5701 gccataggtg gtgccatgag tgccaacggt catcacgctg gcgcactctt cggttgttaa
     5761 caacgcaccg accgggaaac cgccgcccag cgctttggcg gtagttaaca gatcaggtgt
     5821 cacgccgtag tgcatatagg catacagttc cccggtgcgc ccgacgccgg tttgtacttc
     5881 atcaaaaatc aacagcgcat tgtggcggtc acacaattca cgcagacctt gtaaaaacgc
     5941 gttgctggct ggcaccacac cgccttcccc ctggatgggt tcgacaatca ccgcacaggt
     6001 ggcgtcgtca atcagcgcgc tggcagagtt aatatcgtta tatgcagcat gacggatatc
     6061 tggtggcagt ggcgcaaaat cctgtgaata ggctggctgc ccacccgcac tgacagtaaa
     6121 cagcgtgcga ccatgaaacg catttttaaa cgccacgata ccgctcttat ggctgccgta
     6181 gcggtcgtga gcgaatttac gcgccagttt tagcgccgct tcgttggctt ccgcaccgga
     6241 gttacaaaag aagacgcgat cggcaaacgt ggcgtcgatc aattttttcg ccagtcgcag
     6301 taccggctcg ttggtgtaac cgttgccggt atgccagaac ttactcgcct gttcgttcag
     6361 cgcttcacgc agttccggat gcgcatggcc cagcgcgttc accgcaatgc cacccgcgaa
     6421 gtcgatatac tctttcccct gctgatccca caagcgcgaa ccttcgccac gtaccggtat
     6481 aaagggtgcc ggagcgtaaa caggtatcat ccattcatca aagttttcac gcgtaattgg
     6541 ctgagacata gcgacctcta cagtaaataa ttcgctatct atatgttaac aataagtaat
     6601 gtttgcgctg taaatgtaga ttgcagggtt cgtgccagcc agtgataaaa gtgcataaac
     6661 ggcggaggct aactggaaat caaggagtta taaccaaacc atatgcattt aaagtgcata
     6721 taaagtgaat acgtttgcga tgtgggtgaa taaaaagaat aaaaaacgca atgttatgca
     6781 gaagtaaaat ataattctgg aattgtgatc attgacgaaa tttactggaa attactgcgc
     6841 cattctgacg cagcgcgcac caaaagcggg cattttttgc gccatcgttg acatcattaa
     6901 caaccatcga tcaaatcact taacaacagg cggtaagcaa cgcgaaattc tgctaccatc
     6961 cacgcactct ttatctgaat aaatggcagc gactatgaaa tttgtctctt ttaatatcaa
     7021 cggcctgcgc gccagacctc accagcttga agccatcgtc gaaaagcacc aaccggatgt
     7081 gattggcctg caggagacaa aagttcatga cgatatgttt ccgctcgaag aggtggcgaa
     7141 gctcggctac aacgtgtttt atcacgggca gaaaggccat tatggcgtgg cgctgctgac
     7201 caaagagacg ccgattgccg tgcgtcgcgg ctttcccggt gacgacgaag aggcgcagcg
     7261 gcggattatt atggcggaaa tcccctcact gctgggtaat gtcaccgtga tcaacggtta
     7321 cttcccgcag ggtgaaagcc gcgaccatcc gataaaattc ccggcaaaag cgcagtttta
     7381 tcagaatctg caaaactacc tggaaaccga actcaaacgt gataatccgg tactgattat
     7441 gggcgatatg aatatcagcc ctacagatct ggatatcggc attggcgaag aaaaccgtaa
     7501 gcgctggctg cgtaccggta aatgctcttt cctgccggaa gagcgcgaat ggatggacag
     7561 gctgatgagc tgggggttgg tcgatacctt ccgccatgct aatccgcaaa cagcagatcg
     7621 tttctcatgg tttgattacc gctcaaaagg ttttgacgat aaccgtggtc tgcgcatcga
     7681 cctgctgctc gccagccaac cgctggcaga atgttgcgta gaaaccggca tcgactatga
     7741 aattcgcagc atggaaaaac cgtccgatca cgcccccgtc tgggcgacct tccgccgcta
     7801 atttagcagc tctcctggct caaactgggt caggagaatt aaccttgaga aaaatcaaca
     7861 aactgtcagt aatgatttgt tgcctgccgt cctttgttat accgtcactg cgtttttagt
     7921 tgtctgacca cttctctatt atcaagtttg atataggaaa ctccacgatg aatgctgagc
     7981 gtaaatttct tttcgcctgt cttatttttg cgctggtcat ttacgctatc cacgctttcg
     8041 gtttattcga tctgctcacc gatttacccc acttacagac actcattcgc cagagcggat
     8101 tgttcggtta tagcctctat attctgttat tcatcattgc caccctcttt gtgttaccag
     8161 gaagcatact ggtgatcgct ggaggaatag tttttggccc gctcttaggg acactactct
     8221 cattaattgc cgccacgctg gcctcctcgt gctcattcct gctggcgcgg tggctgggac
     8281 gtgatttact gctgaaatac gtgggccata gccatacctt ccaggccatt gaaaaaggca
     8341 ttgcgcgtaa cggtattgat tttcttattc tgacccgctt aatcccgttg tttccttaca
     8401 atattcaaaa ttacgcttac ggattaacca ccatcgcctt ctggccttat acccttattt
     8461 cggcactcac gaccctgccc ggtattgtta tttataccgt gatggcaagc gatctcgcca
     8521 atgaaggcat tacgctgcgc tttattttac aactctgtct ggcgggcctg gcgctgttta
     8581 ttctcgtcca gctcgcaaaa ctctacgccc gacacaaaca tgtggatctg tctgcttcgc
     8641 gccgcagccc gcttactcac ccaaaaaatg aaggatagaa cgatgtcgca acattattca
     8701 gtgtcatgga aaaaaggact ggctgcactc tgcttactgg ctgttgccgg gcttagcggc
     8761 tgcgatcaaa aagagaatgc ggcggcaaaa gtggaatacg acggactttc gaacagccaa
     8821 ccgctgcgcg tcgatgccaa taaccatacg gtaaccatgt tggtgcaaat taatggtcgt
     8881 ttcctcaccg acgacactcg tcacggtatt gtgtttaaag atggctccaa cggacataaa
     8941 tcgctgttta tggcttatgc gaccccgaaa gcattttatg aagccctgaa agaggcaggt
     9001 ggtacgccgg gcgaaaacat gacgatggat aataaagaaa cgactcatgt cacaggcagc
     9061 aaactggata tttcggtcaa ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg
     9121 attgttgaca gtaatggcaa gaaactggac atgcgctttg gcggtaattt aacggcagca
     9181 gaagagaaga aaacgggttg cctggtgtgt ctggatagct gcccggtcgg catcgtcagc
     9241 aatgcaacat acacttatgg tgcggtggaa aaacgcggcg aagtgaaatt caaaggcaat
     9301 gcctcagttc tcccggcgga taacacgctg gcaacggtta cctttaaaat caccgaataa
     9361 agccaggata aaggatgatg aagatgcaat cgcgtaaaat ctggtactac cgtataaccc
     9421 tcatcatcct gttgttcgct atgctgctcg catgggcgct gcttcccggc gtccatgagt
     9481 ttatcaatcg cagcgttgcg gcgtttgccg ccgtggacca acagggtata gaacgcttta
     9541 ttcagtctta cggcgcactg gcggcagttg tctcgttctt gttgatgatt ttacaggcca
     9601 ttgctgcacc gctacctgcg tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt
     9661 tctggggcgg cttgctgtcg tggaccagtt cgatggccgg cgcggcgctg tgctttttta
     9721 tcgccagagt gatgggccgc gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca
     9781 gtatggacgg ctttttcact cgctacggca aacacaccat cctggtctgt cggttattgc
     9841 cttttgtccc tttcgatcca atcagctatg ctgccgatct gacttcaata cgttttcgct
     9901 cgttttttat cgccaccggg cttggtcaat taccgacgac tattgtttat tcctgggcgg
     9961 gcagcatgtt aacaggcggt actttctggt ttgtcaccgg actgtttatt ctgtttgccc
    10021 tgaccgtggt gatttttatg gcgaagaaaa tatggcttga gcgccagaag aggaatgact
    10081 gatgggttta ccgccgctta gcaaaattcc tttcatttta cgtccgcagg cgtggctgca
    10141 tcgtcgccat tacggcgagg tgctaagccc gattcgctgg tgggggcgga tcccgtttat
    10201 cttttatttg gtgtcaatgt ttgttgggtg gctggagcgc aaacgctcac cgctcgatcc
    10261 ggtagtgcga tcgcttgtca gcgcgcgcat tgcgcaaatg tgcctgtgtg agttttgtgt
    10321 ggatatcacc agtatgaaag tcgccgagcg taccggcagc agcgataaac tgctggcagt
    10381 ggctgactgg cggcaaaacc cgctctttag cgatgaagaa cggctggcgc tggagtacgc
    10441 cgaagccgca agcgtcacac cgccaacagt cgatgatgcc ctgcgtaccc gactggctgc
    10501 gcattttgac gctcaggcgc tcaccgaact gacggcattg atcggcctgc aaaacctgtc
    10561 ggctcgtttt aattctgcca tggacattcc cgctcagggg ctgtgccgta ttccggaaaa
    10621 acgttcttaa ggagagaaga tgcgccattg tgggtggttg ctgggattgt tatcgctgtt
    10681 ttctttggca acacatgcca gtgactggca ggaaattaaa aatgaggcca aagggcaaac
    10741 cgtctggttt aacgcctggg gcggcgatac cgcaattaac cgctatctcg actgggttag
    10801 cggcgagatg aaaacccatt acgctataaa cctgaagatt gttcgtctcg cggatgccgc
    10861 agacgcggtg aagcgcattc agaccgaagc tgctgccggt cgtaaaacgg gcggctcggt
    10921 ggatctgctc tgggtgaacg gcgaaaactt ccgcacctta aaagaggcca atttattaca
    10981 aacgggctgg gcggagactc tgcccaactg gcgctatgtc gacacacagc tgccggtgcg
    11041 ggaagatttt tcagtgccga cacaaggtgc ggaatcgccc tggggcggcg cacaactgac
    11101 gtttatcgcc cgccgcgatg ttacgccaca gccaccacaa acgccgcaag ccttactgga
    11161 gtttgctaaa gccaatcccg gcacggttac ttatccgcgc ccaccggact ttaccggcac
    11221 ggcgtttctt gaacagttgc tgattatgct gacgccagat cccgccgcat taaaagaagc
    11281 gccggacgat gcgactttcg cccgtgtcac tgcgcccttg tggcaatatc ttgatgcact
    11341 gcatccgtat ttgtggcgcg aaggaaagga tttcccgcct tcgcccgcgc ggatggatgc
    11401 tctgctgaaa gccggcacat tgcgcctgtc gctgaccttt aaccccgcgc atgcgcagca
    11461 aaaaatcgcc agcggcgatt tgccggcaag cagttacagt tttggctttc gcgaggggat
    11521 gattggcaac gtgcatttcg tcaccattcc agccaacgcg aatgccagtg ctgcggcgaa
    11581 ggtagttgcc aatttcctgc tctcacccga tgcgcaactg cgtaaagcag atcccgttgt
    11641 ctggggcgat ccttctgttc tcgatccgca aaaactgcct gacgggcagc gcgaaatatt
    11701 gcaatcaaga atgccgcagg atctgccgcc ggtactggct gaaccgcacg caggatgggt
    11761 gaatgcactg gaacaagaat ggttacgccg ttacggtacg cattaatctt tttgctgtgg
    11821 gcgatggtgg cggtgattta tgcaccgctg atcccggcag ctctcacgct gatatcgcct
    11881 gcgttgtcgt tgacacactg gcaggcgtta tttgccgatc cacagttacc gcatgcatta
    11941 ctggcaacgc tggtatcgac aaccatcgcg gcggtcgggg cattgttgat tgccctgctg
    12001 gtgattgtgg cgctatagcc tgggccgaaa tggcagcgta tgtgcgcccg tctgccgtgg
    12061 ctgctcgcca ttccccatgt ggcttttgcc accagcgccc tgctgctctt tgctgacgga
    12121 gggctgctat atgactattt cccgtatttc actccgccaa tggaccgatt tggcatcggt
    12181 ctgggcctca cccttgcagt gaaagaaagc gcatttctgc tgtggatctt agcggcagta
    12241 ttgagcgaaa aacggctgtt gcagcaggtc attgtgctgg attcgctggg ctacagccgc
    12301 tggcaatgcc tgaactggct gctgttgccc tccgtcgcgc cttcgctggc aatggcgatg
    12361 ctggcgattg ttgcctggtc gctttcggtc gtggatgtgg cgattattct tgggccaggt
    12421 aatccaccga cgctggcagt aattagctgg cagtggttaa ctcagggcga cgcggatcaa
    12481 caaacgaaag gcgcactcgc cagtctgctg ctgatgctgt tactcgccgc ctacgttttg
    12541 ctgagctatc tgctatggcg cagctggcgg cgcactattc cccgcgtaga tggcattcgc
    12601 aatgcctgcc acacctttat taccaggcac tacgctggcg agttttttac ccttaaccgg
    12661 tgtgctgtgt gtggttctgc tggcgatcct cgcggatcag tcgacgatca atagtgaagc
    12721 actcatcaac agtctgacaa tggggctggt agcaacattc atcgctttgc tcctgttact
    12781 gctgtggctg gaatgggggc cacagcgtcg ccagttgtgg ctatggttgc ccattctatt
    12841 gcccgatctg ccactggtgg cgggccagta cacgctggcg ctatggctga acctggatgg
    12901 aagctggacg gcggtggtct gggggcatct gctgtgggta atgccgtgga tgctgtttat
    12961 cctgcaaccc gcctggcagc gcattgattc acggttaatt ttgattgcgc aaacactggg
    13021 ctggtcgcgg gccaaaatct tcttttacgt gaaatgccca ctcatgttgc gccctgtgct
    13081 gattgccttc gcggtgggat tttcagtcag tattgcgcag tatatgccga cgctgtggct
    13141 gggcgcgggg cgttttccga cgctcaccac tgaggcggtg gcattaagca gcggcggcag
    13201 caacggtatt ctcgccgccc aggctttatg gcaactgttg ttaccgctta ttatttttgc
    13261 cctgaccgcg ttagtcgcaa aatgggtagg ttatgtcaga caaggactcc gctaatgctc
    13321 tgcgtgaaaa atgtttcgct acgtttacca gaaagccgct tgctgacaaa cgttaacttt
    13381 acggttgata aaggtgacat cgtcacgtta atggggccgt ctggctgtgg aaaatccact
    13441 ctgttttcat ggatgattgg tgcactggct ggacagtttt cttgtacagg tgagctatgg
    13501 ctcaatgagc aacggattga catgctaccc accgcacagc gtcagattgg cattcttttc
    13561 caggatgcac tgttatttga ccagttcagc gtcgggcaaa atttactgct ggcgctactg
    13621 tcgacgctca aggggactgc caggcgcaat gccgtgaaag acgcgcttga tcgagcggga
    13681 ctggctgaaa cttatcacca ggaccctgcg acattatcag gcggtcagcg agcgcgggtg
    13741 gccctgctgc gcgcccttct cgctcagcca aaagcgttac tcctggatga gccattcagc
    13801 cgtcttgatg tggctctgcg cgataatttt cgccagtggg tgttcagcga agttcgcgaa
    13861 ctggcgatcc ccgtcgttca ggtaacgcac gatctccagg atgttcctgc tgatagttct
    13921 gttctggata tggcgcagtg gtcagaaaat tacaacaaac tgcgataacg caaagttttt
    13981 ctcaatgcgt cagttcagaa tggcgcactc aaaactacaa tgtcgggatt ttcgatgaaa
    14041 cgtgtttctc aaatgaccgc gctggcaatg gctttagggc tggcttgcgc ttcttcgtgg
    14101 gccgctgaac tggcgaagcc tcttacactt gaccagcttc aacaacaaaa tggcaaagcg
    14161 atagatactc gccccagcgc gttttataac ggctggccac aaaccttaaa tggcccttct
    14221 ggtcatgaac ctgccgcctt aaacctctct gccagctggc tcgacaaaat gagcaccgaa
    14281 cagctcaacg cgtggatcaa gcaacataac ctgaaagccg atgccccggt ggcgctgtac
    14341 ggtaatgaca aagatgtcga cgccgtcaaa acgcgactgc aaaaagcagg ttttacgcat
    14401 atctccattc tgagtgacgc gctaagcgaa ccttcccgtc tacaaaaact gccgcacttt
    14461 gagcagctgg tttatccgca atggctgcac gacctgcaac aaggtaaaga ggttacggcg
    14521 aaacctgccg gtgactggaa agtcattgaa gcggcctggg gcgctcctaa gctttacctt
    14581 atcagccata ttcccggtgc tgactacatc gataccaacg aagtggaaag cgaaccgctg
    14641 tggaacaaag tttctgatga acaactaaaa gcgatgctgg caaaacacgg cattcgccat
    14701 gacaccacgg tcattctgta cgggcgtgac gtatacgctg gagcgcgtgt ggcgcaaatt
    14761 atgctctacg caggcgtgaa agatgtgcgt ctgctggatg gcggctggca aacctggtcc
    14821 gacgcgggac tgcctgttga gcgaggaacg ccaccgaaag tgaaagcgga accggatttc
    14881 ggcgtgaagc tcccggcaca accgcagttg atgctcgata tggaacaggc ccgtggactg
    14941 ctgcatcgcc aggatgcatc gctggtgagc attcgttcgt ggccagaatt tatcggtacg
    15001 accagcggtt acagctatat taaaccaaaa ggtgaaatag ccggagcacg ttggggacac
    15061 gctggtagcg actcgacgca tatggaagat tttcataacc cggatggcac catgcgcagc
    15121 gccgatgata ttaccgctat gtggaaagcg tggaatatca aaccagagca gcaagtttca
    15181 ttctactgcg gcaccggctg gcgcgcgtcc gaaaccttta tgtacgcacg cgccatgggt
    15241 tggaagaatg tttccgtgta tgacggcggc tggtacgaat ggagcagcga tccaaaaaat
    15301 ccggtagcaa ccggtgaacg cggcccggac agtagcaaat aacattgcaa ttactgacgc
    15361 tggagcgact tcagcgtcag atacccactc cacacccgcg taaaggtcgt catccagcac
    15421 aacgcaccga agatccacgc aaaccacgca aaccatgcag ggaataaaca tcccagcaca
    15481 aacagtaaga tcgtttctgt gccttcggtt aatcccccca gatagtaaaa cgacttatgt
    15541 gcataaccgg ggttatcaat ctg
//
LOCUS       DAJTYZ010000092        15407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000092 DAJTYZ010000000
VERSION     DAJTYZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.092"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            83..1246
                     /locus_tag="OO596_002830"
     CDS             83..1246
                     /locus_tag="OO596_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6718639.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1330..2955
                     /locus_tag="OO596_002831"
     CDS             1330..2955
                     /locus_tag="OO596_002831"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6718640.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3072..3347)
                     /gene="yjfN"
                     /locus_tag="OO596_002832"
     CDS             complement(3072..3347)
                     /gene="yjfN"
                     /locus_tag="OO596_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6718641.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3496..3825)
                     /gene="bsmA"
                     /locus_tag="OO596_002833"
     CDS             complement(3496..3825)
                     /gene="bsmA"
                     /locus_tag="OO596_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6718642.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4007..4756
                     /gene="yjfP"
                     /locus_tag="OO596_002834"
     CDS             4007..4756
                     /gene="yjfP"
                     /locus_tag="OO596_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6718643.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(4753..5508)
                     /gene="ulaR"
                     /locus_tag="OO596_002835"
     CDS             complement(4753..5508)
                     /gene="ulaR"
                     /locus_tag="OO596_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6718644.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(5616..6680)
                     /gene="ulaG"
                     /locus_tag="OO596_002836"
     CDS             complement(5616..6680)
                     /gene="ulaG"
                     /locus_tag="OO596_002836"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6718645.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7035..8433
                     /gene="ulaA"
                     /locus_tag="OO596_002837"
                     /pseudo
     CDS             7035..8433
                     /gene="ulaA"
                     /locus_tag="OO596_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8449..8754
                     /gene="ulaB"
                     /locus_tag="OO596_002838"
     CDS             8449..8754
                     /gene="ulaB"
                     /locus_tag="OO596_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6718646.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            8764..9228
                     /gene="ulaC"
                     /locus_tag="OO596_002839"
     CDS             8764..9228
                     /gene="ulaC"
                     /locus_tag="OO596_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6718647.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9242..9892
                     /gene="ulaD"
                     /locus_tag="OO596_002840"
     CDS             9242..9892
                     /gene="ulaD"
                     /locus_tag="OO596_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6718648.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            9902..10756
                     /gene="ulaE"
                     /locus_tag="OO596_002841"
     CDS             9902..10756
                     /gene="ulaE"
                     /locus_tag="OO596_002841"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6718649.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            10756..11442
                     /gene="ulaF"
                     /locus_tag="OO596_002842"
     CDS             10756..11442
                     /gene="ulaF"
                     /locus_tag="OO596_002842"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6718650.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            11539..12090
                     /locus_tag="OO596_002843"
     CDS             11539..12090
                     /locus_tag="OO596_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR6718651.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12165..12480)
                     /locus_tag="OO596_002844"
                     /pseudo
     CDS             complement(12165..12480)
                     /locus_tag="OO596_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            12807..13202
                     /gene="rpsF"
                     /locus_tag="OO596_002845"
     CDS             12807..13202
                     /gene="rpsF"
                     /locus_tag="OO596_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6718652.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13209..13523
                     /gene="priB"
                     /locus_tag="OO596_002846"
     CDS             13209..13523
                     /gene="priB"
                     /locus_tag="OO596_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6718653.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13528..13755
                     /gene="rpsR"
                     /locus_tag="OO596_002847"
     CDS             13528..13755
                     /gene="rpsR"
                     /locus_tag="OO596_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6718654.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13797..14246
                     /gene="rplI"
                     /locus_tag="OO596_002848"
     CDS             13797..14246
                     /gene="rplI"
                     /locus_tag="OO596_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6718655.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14358..15329)
                     /locus_tag="OO596_002849"
     CDS             complement(14358..15329)
                     /locus_tag="OO596_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR6718656.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
BASE COUNT         3568 a         3786 c         4124 g         3929 t
ORIGIN      
        1 ttaccaccaa aaaagcgtca accgtttcgc gcggcggcta tggtcgttct tccagcgccc
       61 gtgggcattg gggaggctaa tcatgctgag acacaacgtt cctgtgcggc gggatctgga
      121 ccaaattgcc gctgacaacg gtttcgactt tcatatcatc gacaatgaaa tctattggga
      181 cgagagtcgg gcttaccgtt ttactctgcg ccagattgaa gagcagatcg aaaaaccaac
      241 tgcggaactg catcagatgt gccttgaggt ggtggatcgc gcggttaaag acgaagagat
      301 cctgacgcaa ctggcgatcc cgccgttgta ctgggatgtg atcgccgaaa gctggcacgc
      361 cagcgatcct tcgctgtatg gccggatgga ttttgcctgg tgtggcaatg cgccggtgaa
      421 gctgctggag tacaacgccg atacgccgac ttcgttgtat gagtcggctt atttccagtg
      481 gttgtggctg gaggatgccc ggcgcagcgg cattattccg cgtgatgccg atcagtacaa
      541 tgccattcag gaacgcctga tttcacgctt tagcgagctt tatagccggg agccgtttta
      601 tttctgctgc tgtcaggaca ccgatgaaga caggactacc gtgctgtact tgcaggactg
      661 cgcccagcag gcagggcagg agtcgcggtt tatctacatt gaagatctcg gtttgggcgt
      721 cggcggtgta ctgaccgatc ttgatgataa tgtcatccag cgtgcattta agctgtatcc
      781 gctggagtgg atgatgcgtg acgataacgg tccgctgctg tgcaagcgtc gcgagcaatg
      841 ggtggagccg ttatggaaaa gtatcttgag taataaaggg ctaatgccgc tgctttggcg
      901 cttcttccct ggtcatccta accttcttgc atcctggttt gagggtgaaa aatcgcagat
      961 tgccgctggc gaaagctatg tgcgtaaacc gatctactcg cgcgaaggcg gaaacgtcac
     1021 cattttcgac ggtcagaata acgtcgttga ccacgctgat ggtgattacg ccgatgaacc
     1081 gatgatctac caggcgtttc aacctctgcc gcgatttggc gatagctaca cactcatcga
     1141 tagctggatt gtcgatgatg aagcgtgcgg aatggggatc cgtgaagata acacactgat
     1201 caccaaagac acctcacgtt tcgttccgca ttacattgct ggataagaat gttttagcaa
     1261 tctctttctg tcatgaatcc atggcagtgg ccatactaat ggtgactgcc attgatggag
     1321 ggagacacag tgcactggca aactcacacc gtttttaatc aacctatacc attaaataac
     1381 agcaacttat acctgtctga tggcgcgctt tgcgaagcgg taacgcgtga aggtgctggc
     1441 tgggatagcg attttctagc cagtattggt cagcagttag gaacggctga atcccttgaa
     1501 ctggggcggc tggcgaatgt aaacccgcct gaactgttgc gctacgatgc ccaggggcgg
     1561 cgtctggacg atgttcgttt ccatcccgca tggcacctgc tgatgcaggt gttatgtacc
     1621 aatcgggtgc acaatcttgc ctgggaagaa gacgctcgct ccggcgcatt tgtggcgcgc
     1681 gcggcgcgtt ttatgttaca tgcgcaggtt gaggctgggt cgttatgtcc aataacgatg
     1741 acctttgccg ccacgccatt gttgttacag atgttaccca cgccgtttca ggactggacc
     1801 acgccgctgc tgagcgatcg ctacgattct cacttattgc caggtgggca aaaacgcggt
     1861 ttgttgattg gcatgggaat gacggaaaag cagggtggtt ccgatgtcat gagcaacacc
     1921 acccgtgcag agcgtttgga agatggctct tatcggctgg tggggcataa atggtttttc
     1981 tcggttccgc aaagcgatgc gcatctggtg ctggcgcaga ccacgggcgg tctgtcctgt
     2041 ttttttgtgc cgcgcttttt gcctgacggg caacgcaacg cgattcgcct cgaacggctg
     2101 aaagataagc tgggtaatcg ctctaacgcc agttgtgaag tggagtttca ggatgccatt
     2161 ggctggttat tggggcagga aggggaagga attcgtctga tcctgaaaat gggggggatg
     2221 acgcgttttg attgcgccct gggtagccat gccatgatgc gccgtgcgtt ttcgctggcg
     2281 atttatcatg cacatcaacg ccatgttttt ggtaatccat tgatccaaca gccccttatg
     2341 cgtcatgtct taagtcgcat ggcacttcag cttgaagggc aaacggcgtt gctgtttcgt
     2401 cttgcgcgag cgtgggaccg gcgcgccgat gccaaagaag ccctgtgggc gcgtttattt
     2461 acgcctgcgg caaaatttgt gatctgcaaa cgaggtatgc cgtttgtggc cgaagcgatg
     2521 gaggtgctgg gcggcattgg ttattgcgag gagagcgagc tgccgcggct ttaccgggag
     2581 atgccggtaa acagtatttg ggaaggttcc ggcaatatta tgtgcctgga tgtgttgcgc
     2641 gttctcaata agcaagcggg cgtatacgac ttattgtcgg aagcatttgt ggaagtgaaa
     2701 gggcaggatc gctattttga tcgcgcggtt cgtcgtttac agcagcagct gcgtaagcca
     2761 gctgaagaac tggggcgaga gattactcat cagctattcc tgctgggctg cggtgcgcaa
     2821 atgttgaaat atgcttctcc gccaatggcg caggcgtggt gtcaggtgat gttagatacg
     2881 cgcggcggcg tacggttgtc agagcagatc cagaatgatt tattgctgcg ggcgacgggg
     2941 ggagtgtgtt tgtaaacgta tacgactgat gcgacgctgg tttcgattaa ctcaatgaaa
     3001 tatgtgaaaa ttgtaggccg gacaaggcgc tcgcgccgca tccggcattg ttcatttgct
     3061 gtagatgaca ctcatgcata cagtatcgcc tgtacgcgcc agttatcagg ctgctggtct
     3121 tcgtacatgg taataatgcg gtaccatgac gcaccttgtt catcggcctt taacgccacg
     3181 atacgttcca cgtcctgcgg atcccctgaa atattgctaa ctgagatctg acctatttca
     3241 ttcaggcccg tcacgcagtc agcactggcg aattctgcag actgtgccgt ggcgctcgca
     3301 ggataagtca ctaattgcag cgagggtgag gcaagtaatt gtttcatcgt cagctccatt
     3361 tttcgtttcc ccacatgttg cagggcattc catcgcggta ataaacgccc aggcttccag
     3421 ccggtaattt attgtgcaca ggtaaacgca tagggacgca aagcaatgta aagctgacta
     3481 tgtaagttgt ctgatttatt tacggtacaa aatggcttgt gaataccact gtcctgtcac
     3541 gatggtttcg tccaccatga cgacaacgta ataatcagct tttgcagcga cagcttttgc
     3601 tctgatttct gctaatgcgt catccggaga accccgaacc atcgtgctta cgctacctat
     3661 tcgctgtaac ccttgcgtct ggtcgcggcg aatctcttgc ggatggtccg ttactggcgg
     3721 tgctggctgt ggcgtacctt gcagtgcgct acaggcactt aacatcaaca ccaataataa
     3781 actggcaaac cggtaaataa cgctattacg tttcctgcta accatagtgt agtgccttat
     3841 taatttaact ttgggggaat gttcccgaat tgttacctgg tacgcgattt tcgaatgaaa
     3901 acgtgacgaa agcgtaaccc acatctcaac attatgaact aataccgcca gcagaaactt
     3961 gtgctaagga tttcttcatg tcataccaga caaaaaggag agacagatga ttgaaataga
     4021 atcacgcgag ctggcagata ttcccgttct tcatgcttat cctgtcgggc aaaaagatac
     4081 cccgttaccg tgcgtaattt tttatcacgg ctttacttca tccagcctgg tgtatagcta
     4141 ttttgccgtt gcgctggcgc aggctggttt gcgggtgatc atgccggatg cgcccgatca
     4201 cggtagccgt tttagtggcg acgaagcgcg gcggttaaat caattctggc aaatcttgct
     4261 acaaagtatg caggaattca ctactttacg tgcggcaata gccgaagaaa actggctgct
     4321 tgatgaccgt ctggcagtcg gtggcgcgtc gatgggcgcg atgacggcac tgggaattac
     4381 cgctcgccac cccacggtga aatgtaccgc cagcatgatg ggatcgggct attttacatc
     4441 gctcgcccgt tcactgtttc caccgctgat acctgaaacg gcagcacagc agaatgaatt
     4501 caataacatt atcgcgccgc tggcagagtg ggaagcgaca aaccacctgg aacaacttgg
     4561 ggacagacct ctactgctgt ggcatggcct cgacgatgac gttgtgcctg ccgacgaatc
     4621 actacgtttg cagcaggcct taagcgagac gggacgggat aaactgctaa cctgttcatg
     4681 gcagccaggc gtgcgtcacc gcattacgcc tgaggcgtta gatgctgccg tgacattttt
     4741 ccgccagcat ctttaaacac gcagaatgct gaccccttgc gcttccagtt gttgcaggat
     4801 ttccgggtta gcatttttgc cggtgataag catatcgatt tgatcggcac ggctaaaaag
     4861 cattcccgcg cgttcgccaa tcttactgct atcaaccagt accaccagtt tccctaccac
     4921 gctcagcatc ttctgctctg ccattgctgt cagcatatcg gttttataca acccttctgc
     4981 ggtcagccct tttccgctgg taaacatcca gtgcccggca tagagactgt tttcgctgcc
     5041 ctgcgggctt aaagtgatgg actgactttt gttgtactgt ccacccataa tgatcacgct
     5101 gtcatgttct tgatcgatca ggtaatttgc cagcggtaga taattagtga tgatttgcac
     5161 tggcttgcca cacatttccc gcccaagcag aaacgcggtg gagccgcagt tgatgactac
     5221 gctttcgccc ggattaacca gctgcgaggc cgctttagca atacgtactt tttcatcgtg
     5281 attctgcgcc tgatgcagat tcatcggcgt ccagcgcggg cgctgttggg taatagcttc
     5341 tgcgccattg cgcacctttt tcagtttgcc gcgttcgtca agtttattga tatcgcgtcg
     5401 cgcagtggca ggcgaaattc ccagacgctc aacgactttc tcaacggtca caaagcccaa
     5461 ttgtgcgagc atttccagga ggatttgatg tctttgtgct tcagtcatga gctattccga
     5521 taaaatttga tttttttaga tgatatttga aatagctagg aaatactacg ccggatagcg
     5581 cgaacttctc cacgctatcc ggtataaacg acagattaca ggaatgactt gaacggcaga
     5641 tccggttcaa tagtgaagca atcatcgaaa ccgcgcggat agtggtactc gaagttgtct
     5701 ttatccagcg gccaggtaaa tttgccgcca acctgccaga taaacggctt gaagccatac
     5761 ttcagacgat cttttttcat ctcccacagc acgcggatct cttgcggatc ggcctggaag
     5821 tttgaccaga tgtcgtggtg gaacgggata accactttcg cattcagggc ttcacccata
     5881 cgcagcatat cggcgctggt cattttgtcg gtaataccgc gcgggttctc accatacgat
     5941 cctaacgcca catcgatctg atgttcgtta ccgtgcttcg cataatagtt agagtagtgg
     6001 gaatcgccac tgtgatatag ggaaccgcca ggggttttga acaggtagtt tactgcgcga
     6061 tcgtccatgc catctggcag tacgccagcc gctttttgat cggcaggcag ggtgatcagt
     6121 gcagtacggt cgaaagcgtc aagcgcatga atttcaatgt ctttcacttt tacaacatcg
     6181 cccggtttga ccacgatgca acgctctttc ggtacgcccc agccaatcca caaatcaacg
     6241 caggttttcg gtccaatgaa tggtacgtca tcagcacagt tctgcatcac ggcagcagcg
     6301 acgttaacgt cgatatgatc gttgtgatcg tgagtcgcca gtaccgcgtc gatctggcga
     6361 atcgcaaacg gatcaagaac aaacggggtg gtgcgtaggt ttggctgcag ttttttcacg
     6421 ccagccatgc gctgcatctg gtgaccctgt ttcattaacg ggttaccgtg actttgtttg
     6481 ccagtgccgc accagaaatc aacgcaaacg ttggtgccac cttctgattt caaccagatc
     6541 ccggtgcagc ctagccacca catcgcaaat gtgccagggg cgacctgctc ttgttcaatt
     6601 tcttcattca gccagctacc ccactccggg aaagtgctca ggatccagga ttctcgggtg
     6661 atacttttca ctttactcat cgccatttac cttcatgata gttcaattcg aatcaatatg
     6721 tgattggttt tgattaatcc tgacactatt ttttcaggaa ggcaatgacc attttttgac
     6781 tttttgccag ggaagttgtt gttgattttg agtgtggaaa gatttaatgg aatgtgtagt
     6841 taaattaact aaatgaattt aaataaataa ttgtttcgtt gtgtgaatcc cactctatcc
     6901 gtgtggaatt atttgcgggt cgcgtcacat ttaatcataa ataatcttgt tgtgattact
     6961 tttgaaaatt agagtgagtg cacaacattc cgggtgtgcg gaatacccgg ttacctcttc
     7021 ttcaggagat cgttatggag atcctctaca acatctttac cgtgtttttt aaccaggtca
     7081 tgaccaatgc cccgttgttg ctgggtattg tgacctgtct gggctacatc ctactgcgca
     7141 aaagtgtcag cgttattatt aaaggcacga ttaaaaccat aattggtttc atgttgttgc
     7201 aggcagggtc cggcatcctc accagcacct tcaaaccggt ggtggcgaaa atgtccgaag
     7261 tctacggcat taaacggcgc aatttccgat acttacgcgt cgatgatggc aaccatcgac
     7321 cgcatgggcg atgcctatag ctgggtaggt tacgcggtgt tgttagcgct ggcgctgaac
     7381 atctgttacg tgctgctgcg tcgcattacc ggtattcgta ccatcatgct gactggtcat
     7441 atcatgttcc agcaggcggg gctgattgcc gttacgttgt ttattttcgg ctactccatg
     7501 tggacaacca ttatctgcac ggcgattctg gtttcgctct actggggcat tacctccaac
     7561 atgatgtaca agccgactca ggaagtgacg gacggctgcg gtttctccat cggtcaccag
     7621 caacagtttg catcatggac tgcctataaa gttgcgccgt tcctcggcaa aaaagaggaa
     7681 agcgttgaag acctcaaact gccaggctgg ctgaatattt tccacgacaa catcgtctcc
     7741 acggcgattg tcatgaccat cttctttggt gccattctgc tctccttcgg tatcgacacc
     7801 gtgcaggcga tggcaggcaa agtgcactgg acggtctata tcctgcaaac cggtttttcc
     7861 ttcgcggtgg cgatcttcat catcactcag ggtgtgcgca tgtttgtggc ggaactctct
     7921 gaagcgttta acggtatctc ccagcgcctg atccctggtg cggttctggc gattgactgt
     7981 gcggctattt atagcttcgc gccgaacgcc gtggtctggg gctttatgtg gggcaccatc
     8041 ggtcagctga ttgcggttgg catcctggtc gcctgcggtt cctcgagcct gattatccca
     8101 ggctttatcc cgatgttctt ctccaacgcc accatcggcg tgttcgctaa ccacttcggc
     8161 ggctggcgtg cggcgctgaa gatttgtctg gtgatgggga tgatcgaaat ctttggttgc
     8221 gtctgggcgg tgaaacttac cggtatgagt gcctggatgg ggatggcgga ctggtcgatt
     8281 ctggcaccgc cgatgatgca gggcttcttc tccatcggta tcgcctttat ggccgtcatc
     8341 attgtaattg cactggctta tatgttcttc gctggccgcg cactgcgcgc agaagaagat
     8401 gcagaaaaac aactggcaga acagtctgct taataaggag ttttgattat gaccgtacgt
     8461 attctggctg tgtgtggcaa cggacaaggc agttccatga tcatgaggat gaaagtggac
     8521 cagtttttaa cccaatcaaa cattgaccac acggtaaata gctgcgcggt tggcgaatac
     8581 aaaagcgagt tgagcggcgc ggatatcatc atcgcttcta cgcacattgc gggcgaaatc
     8641 accgtgaccg gcaacaaata cgtggttggc gtgcgcaaca tgctctctcc tgccgacttt
     8701 ggcccgaaac tgctggaagt gatcaaagcg catttcccgc aggatgtgaa gtaaggacgc
     8761 gccatgaaat tacgtgattc gctggcggag aataaatcca tccgcctgca ggctgaagct
     8821 gagacatggc aggacgcagt aaaaatcggc gttgacctgc tggtggcggc ggatgttgtc
     8881 gagccgcgtt actaccaggc gattctggat gcggttgaac agcacggtcc gtactttgtg
     8941 ctcgcaccag gcctggcaat gccgcacggt cgcccggaag agggcgttaa gaaaaccggt
     9001 tttgcgctgg tgacgctgaa aaagccgctg gagttcaacc acgaagataa cgacccggtg
     9061 gatatcctca tcaccatggc ggcggtcgat gccaacacgc accaggaagt gggcatcatg
     9121 cagatcgtca acctgtttga agatgaagaa aatttcgacc gtttacgtgc ctgccgtacc
     9181 gagcaggaag tactggatct cattgaccgc accaacgcgg cagcttaaga aggaattgaa
     9241 gatgtcatta ccgatgttgc aagtcgcgct ggacaaccag actatggata gcgcctacga
     9301 aaccactcgc ctgattgccg aagaagtcga cattatcgaa gtgggtacca ttctgtgcgt
     9361 gggcgaaggc gtgcgtgcgg ttcgcgatct gaaagcgctc tacccgcaca aaatcgtgct
     9421 ggcagacgcc aaaattgccg atgcgggcaa aattctttct cgtatgtgct tcgaagccaa
     9481 cgctgactgg gtgaccgtga tttgttgtgc ggatatcaac accgccaaag gtgcgctgga
     9541 cgtagcaaaa gagtttaacg gcgacgtgca gatcgaactg accggttact ggacctggga
     9601 acaggcgcaa cagtggcgcg acgcaggtat tcagcaggtg gtttatcacc gcagccgtga
     9661 cgcgcaggcc gctggcgtgg cgtggggtga agccgatatc actgcgatca aacgtctttc
     9721 cgatatgggc ttcaaagtca ccgtcaccgg tggcctggcg ctggaagatc tgccgctgtt
     9781 caaaggcatt ccaattcatg tgtttatcgc cggtcgcagc atccgtgatg ccgcttctcc
     9841 ggttgaagcc gcgcgtcagt tcaaacgttc catcgccgaa ctgtggggct aaggagcgga
     9901 tatgttgtcc aaacaaatcc cgcttggcat ctatgaaaaa gcgctccccg ccggggagtg
     9961 ctggctggaa cgcctgcgac tggcaaaaac gttgggcttc gattttgtcg agatgtcggt
    10021 agatgaaact gacgagcgcc tgtcgcgcct cgactggagc cgcgagcagc gtctggcgct
    10081 ggtgaatgcg attgttgaaa ccggcgtgcg tgtgccgtcc atgtgccttt ctgcccatcg
    10141 tcgtttcccg ttgggcagcg aagatgacgc ggtgcgggcg caggggctgg agattatgcg
    10201 taaagccatc cagttcgccc aggacgttgg cattcgcgtg atccagctgg cgggctatga
    10261 cgtttactat caggaagcca ataacgaaac gcgtcgtcgt ttccgtgacg gcctgaaaga
    10321 gagcgttgag atggcgagcc gcgcgcaggt gacgctggcg atggagatca tggattatcc
    10381 gttgatgaac tccatcagca aggcgctggg ttacgcgcac tatctcaaca acccgtggtt
    10441 ccagctctac ccggatatcg gcaacctgtc ggcgtgggac aacgatgtgc agatggagtt
    10501 gcaggccgga atcgggcata tcgtcgcggt gcatgtgaaa gacaccaaac cgggcgtctt
    10561 caaaaacgtg ccatttggcg aaggtgtggt ggatttcgaa cgttgcttcg aaacgctcaa
    10621 acagagtggc tattgcgggc cgtacctgat tgagatgtgg agcgaaacgg cggaagaccc
    10681 ggcggcagaa gtggtgaaag cgcgtgattg ggtgaaagcg cgcatggcca aagcaggcac
    10741 ggtggaggcg gcataatgca aaagctaaaa cagcaggtgt ttgaagccaa catggatctg
    10801 ccgcgctatg ggctggtgac ctttacctgg ggcaacgtca gcgctatcga ccgcgaacgc
    10861 gggctggtgg tgatcaagcc cagcggcgtt gcctacgaaa ccatgaaagc agacgatatg
    10921 gtggtggtcg atatgagcgg caaggtggtg gaaggggagt atcgcccgtc ttccgacacc
    10981 gcgacgtatc tcgaactcta ccgtcgttac ccgtcgcttg gcggcattgt ccatacccac
    11041 tccactcatg ctactgcatg ggcgcaggcg gggctggcga tcccggcgtt aggcactacg
    11101 cacgccgact acttctttgg cgacatcccg tgtacgcgcg gattaagcga agaagaggtg
    11161 cagggcgagt atgaactgaa caccggcaaa gtgattatcg aaacgctggg tgacgccgag
    11221 ccgctgcata cgccggggat tgtggtgtat cagcacggtc cgttcgcctg ggggaaagat
    11281 gcccacgacg cggtgcataa cgcggtggtg atggaagaag tggcgaaaat ggcgtggatt
    11341 gcgcgcagta ttaacccaca actgaatcac atcgacagct tcctcatgaa taaacacttc
    11401 atgcgtaagc acggtcctaa cgcctattac gggcagaagt aagcagtgcg ccccggaatt
    11461 attacctcct gcgggatgta attggtctga ttccggggcc gcttcagtta aaaaatcgta
    11521 taaacaacag gtgtttttat ggctatcaat ggattgtttg tcagaacgga tccgcaacgt
    11581 cgccgctatg gcgttgcgtt acttgtgggt gtactttcag gaattatttc agcatttgtt
    11641 aaatggggag cggaagtccc cttgccaccg cgcacatttt ccggtggtcg cgatgaattt
    11701 aacccgccgt atatctttct acgggattat ttaggtatcg acccaaccca aactgtgtat
    11761 acattctcgg aacatattat caacccggtg atggtgacgc atatcatctt ctcactggtt
    11821 tttgcgatag ggtattgtat tgtcgcggaa attttcccga aagtgaaatt atggcagggg
    11881 atattagcag ggcttattgt gacagtggca gtccacggta tattttgccc ggcgctgaat
    11941 ttaacgccgc cgctcactca attgcctttt gacgaatatg cctctgaaat cctggggcat
    12001 attttctggt tctggataat agaaattatg cgccgcgact tacgtaatcg cattactcat
    12061 gagccagatc cggaagtcgc aatccggtga ataaccgcat aaaaaagccc cctgcgaagg
    12121 gggcaaagca aactatggca atgtttcgtt ggttatacct ggtgctagcg ataaatatcc
    12181 gcgctggcgt gcatattgcc gttactcccc ggttcccgca tcagaattac gtggtagtac
    12241 gttactcccc ggttcccgca tcagaattac gtggtagtac gttgcgccct gcgcatctgt
    12301 ctcttcattt aatgcctgac gcgcttcgct ttcagtggcg aaattatgat tgatataaat
    12361 cacgcctaag ctttgcacat catccatgtt tctggcctgg tggttattaa tttcaatggc
    12421 tgcccatgta tttgcactta gcaaaagcac agccagaagg gctaaaactc gactgaacat
    12481 agatacctcc tcgacggctg actttgtgtg ctctcctctg tgatgatctt ctgatttaat
    12541 tttaatcaat gataaagaag ttgatggtga ccatttctga tgcagttgtt caaaaaaaca
    12601 ccatgatgaa gtgtgatgaa ctccaaatca gcggtttaga ggttaattgc gaaaggggag
    12661 atttatttcg gctctgccct tgagtttagc ggggcataca agtactataa cgcgtcattt
    12721 ttcagccgac ctttaacacg ttccttgcct ccccgggatt cggctgaccc agacaggagg
    12781 ctgaataatc cgtaaggagc aattcgatgc gtcattacga aatcgttttt atggtccatc
    12841 ctgaccagag cgaacaggtt ccgggcatga tcgagcgcta cactgctgcc atcactggtg
    12901 cagaaggcaa gatccaccgt ctggaagact ggggccgccg tcagctggct tacccgatca
    12961 acaaactgca caaagcacac tacgttctga tgaacgttga agctccgcag gaagtgatcg
    13021 atgagctgga aactaccttc cgcttcaacg atgccgttat ccgcagcatg gttatgcgta
    13081 ccaagcacgc tgttaccgaa gcatctccga tggttaaagc gaaagacgag cgccgtgagc
    13141 gtcgcgatga tttcgcaaac gaaaccgctg atgatgctga agctggggat tctgaagagt
    13201 aatttctgat gaccaaccgt ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa
    13261 aggtcagtcc atcaggaatt cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg
    13321 aggaagccgg ttttcaccgg caggcgtggt gccaaatgcc cgttattgtt agcggacacg
    13381 aaaaccaggc cattactcac agtataacgg tcggcagtcg cataaccgtt caggggttca
    13441 tttcatgcca caaggcaaag aacggactga gcaaaatggt tttgcatgcc gagcagattg
    13501 aattgataga ttctggagac tagccatatg gcacgttatt tccgtcgtcg caagttctgc
    13561 cgtttcaccg cggaaggcgt tcaagagatc gactataaag atatcgctac gctgaaaaac
    13621 tacatcaccg aaagcggtaa gattgtccca agccgtatca ccggtacccg tgcaaaatac
    13681 cagcgtcagc tggctcgcgc tatcaaacgc gctcgctacc tgtccctgct gccgtacact
    13741 gatcgccatc agtaatcggt cacggtccat taatacgact ttgagaggat aaggtaatgc
    13801 aagttattct gcttgataaa gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta
    13861 aagcgggcta tgctcgtaac ttcctggtac cgcagggtaa agctgttcca gctaccaaga
    13921 aaaacattga attcttcgaa gcacgtcgcg ctgaactgga agctaaactg gctgaagttc
    13981 tggcagctgc taatgctcgc gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt
    14041 ctaaagctgg cgacgaaggt aaactgttcg gttccatcgg tactcgcgac atcgctgacg
    14101 ctgtaactgc agctggcgtt gaagtggcta agagcgaagt tcgtctgccg aacggcgttc
    14161 tgcgtaccac tggcgagcac gaagtgagct tccaggttca cagcgaagta ttcgcgaaag
    14221 tgatcgtaaa cgtagtagct gaataattcg ttattcaacg agacgtaaaa acgccgacca
    14281 gtggtcggcg ttttgctttt gtagcgagca cttgcagata tcgataaggg ccacaataag
    14341 cagccctacg aagataatta cttagctgcc tgaatatcag ccagcattgg ggcaagggct
    14401 gggttgtcct tagcgaagtt tgcgtacatt ggctgtactt tctcgataaa cggagcttta
    14461 tctacctggt agactttcac gcccatttta tccagttttg caaattcttg tttctcttcc
    14521 tcgctccaca agtctttcat gtagctcatt gattctttag ctgcttttaa tataactgcc
    14581 tgattttccg ggctaagttt atcaaacgca gcattgctca tcagaaccac atcgggaacc
    14641 atagtgtgtt cgtcaaggct gaaggatttt gccacttcac cgtgacgcat ggtggtcagc
    14701 gccatcacgc tgttttctgc catatcgacc acaccctgct gtaaggctgt atacaactcg
    14761 ccgtaatcca gtggcgtcgg gttaccgccc atgactttga tcatttcaac tgcgccaggg
    14821 cttggctgga cacggacttt catgcctttc agatcgtctg gtttcagtac tggtttattg
    14881 ccatagaaac tgcgggcgcc tccatcataa aaagtcagcc cgaaataacc tttgcttttc
    14941 gttgagtcga ggatttttct cccgatatcg ccctgcatga cctgataata atgatcacga
    15001 tcgcggaaca aataaggcag gctaaataat ttataggaat tttcaaatgc ttccatttct
    15061 gccgcattgg tttttaccag tggaatagcg ccagaacgaa tcagctccat tgtttctcgt
    15121 tgagtaccca atgttccatt tggataaata cgaatagtaa tatcaccgtt agagtactct
    15181 ttgttctttt tcgcaaagaa ctccatcgct ttatgaacag gatgagactt atcctgatta
    15241 tgactcagtt ttaatgtcac tgcccaggac tggcttgcgg ataacataag aacagcaccg
    15301 ataatgccag cggatacttt tattttcata gatgttcctt ttctaataaa tatggcggca
    15361 ggtgccgcca gtgaatgcgc atcagtagcc cagtgctttt ggcaatg
//
LOCUS       DAJTYZ010000093        15252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000093 DAJTYZ010000000
VERSION     DAJTYZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.093"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1745
                     /locus_tag="OO596_002850"
     CDS             <1..1745
                     /locus_tag="OO596_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6718657.1"
                     /translation="LFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVP
                     PGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSH
                     IEGASKHSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKS
                     HSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYR
                     RDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQ
                     LSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTT
                     FDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYS
                     QSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNG
                     VVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIK
                     ALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTIT
                     FGKEIDESRNYICQ"
     gene            1761..2795
                     /locus_tag="OO596_002851"
     CDS             1761..2795
                     /locus_tag="OO596_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCR6718658.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(2877..3215)
                     /gene="rcnB"
                     /locus_tag="OO596_002852"
     CDS             complement(2877..3215)
                     /gene="rcnB"
                     /locus_tag="OO596_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6718659.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3434..4258)
                     /gene="rcnA"
                     /locus_tag="OO596_002853"
     CDS             complement(3434..4258)
                     /gene="rcnA"
                     /locus_tag="OO596_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR6718660.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4379..4651
                     /gene="rcnR"
                     /locus_tag="OO596_002854"
                     /pseudo
     CDS             4379..4651
                     /gene="rcnR"
                     /locus_tag="OO596_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      4743..4839
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4874..5662
                     /gene="thiM"
                     /locus_tag="OO596_002855"
     CDS             4874..5662
                     /gene="thiM"
                     /locus_tag="OO596_002855"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6718661.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5659..6459
                     /gene="thiD"
                     /locus_tag="OO596_002856"
     CDS             5659..6459
                     /gene="thiD"
                     /locus_tag="OO596_002856"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6718662.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6524..7342
                     /locus_tag="OO596_002857"
     CDS             6524..7342
                     /locus_tag="OO596_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6718663.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7394..8140
                     /locus_tag="OO596_002858"
     CDS             7394..8140
                     /locus_tag="OO596_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6718664.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8114..9079)
                     /locus_tag="OO596_002859"
     CDS             complement(8114..9079)
                     /locus_tag="OO596_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6718665.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9076..10080)
                     /locus_tag="OO596_002860"
     CDS             complement(9076..10080)
                     /locus_tag="OO596_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR6718666.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10077..11354)
                     /locus_tag="OO596_002861"
     CDS             complement(10077..11354)
                     /locus_tag="OO596_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6718667.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11611..12663
                     /gene="fbaB"
                     /locus_tag="OO596_002862"
     CDS             11611..12663
                     /gene="fbaB"
                     /locus_tag="OO596_002862"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6718668.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            12982..13836
                     /gene="gatY"
                     /locus_tag="OO596_002863"
     CDS             12982..13836
                     /gene="gatY"
                     /locus_tag="OO596_002863"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6718669.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            13865..15122
                     /gene="gatZ"
                     /locus_tag="OO596_002864"
                     /pseudo
     CDS             13865..15122
                     /gene="gatZ"
                     /locus_tag="OO596_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15132..>15252
                     /locus_tag="OO596_002865"
     CDS             15132..>15252
                     /locus_tag="OO596_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6718670.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQAL"
BASE COUNT         4000 a         3668 c         3903 g         3681 t
ORIGIN      
        1 ggttgtttcg tgatatgcag atgttgccta actcgaaaca aaattttacg ccacgggtgc
       61 aggggattgc tcagagtaac gcgctggtaa ctattgaaca gaatggtttt gtggtttatc
      121 agaaagaggt tcctcctggc ccgtttgcga ttacagattt gcagttggcc ggtggtggag
      181 cagatcttga tgtcagcgtg aaagaggcgg acggttcggt aaccacctat ctggtgcctt
      241 atgcagcggt gccaaatatg ctgcaacccg gcgtgtcgaa atatgatttt gcggcgggtc
      301 gtagccatat tgaaggggcg agcaaacata gtgattttgt ccaggcgggt tatcagtatg
      361 gttttaataa cttattgacg ttgtatggtg gctcgatggt tgcgaataat tattacgcgt
      421 tcactttggg gactggctgg aatacacgca ttggtgccat ttccgtcgat gccacgaagt
      481 cgcatagtaa acaagacaac ggcgatgtgt ttgacgggca aagttatcaa attgcctaca
      541 acaaatttgt gagccaaacg tcgacgcgtt ttggtctggc ggcctggcgt tattcgtcgc
      601 gtgattaccg gacatttaac gatcacgttt gggcaaacaa taaagataat tatcgccgtg
      661 atgaaaacga tgtctatgac attgccgatt attaccagaa tgattttggt cgcaaaaata
      721 gcttttccgc caatatgagc cagtcattgc cagaaggttg ggggtctgtg tcattaagta
      781 cgttatggcg agattactgg gggcgtagcg gcagtagtaa ggattatcag ttgagttatt
      841 ccaacaacct gcgacggata agctataccc tcgcggcaag ccaggcttat gacgagaatc
      901 atcatgaaga gaaacgtttt aatattttta tatcgattcc ctttgattgg ggtgatgacg
      961 tttcgacgcc tcgtcggcaa atatatatgt ctaactcaac gacgtttgat gatcaggggt
     1021 ttgcctcaaa taatacggga ttatcaggaa cagtagggag tcgggatcag ttcaattatg
     1081 gtgtcaacct gagtcatcaa catcagggaa atgaaacgac agctggagcg aatttaacct
     1141 ggaacgcgcc ggtggcaaca gtgaatggca gttatagtca gtcgagtact tatcgacagg
     1201 ctggagccag tgtttcaggg ggcattgtcg cctggtcggg tggcgttaat ctggcgaacc
     1261 gtctttccga aacgtttgct gtgatgaatg cgccaggaat taaagatgct tatgtcaatg
     1321 ggcaaaaata tcgcacaaca aaccgtaatg gagtggtggt atacgacgga atgacacctt
     1381 atcgggaaaa tcacctgatg ctggatgtgt cgcaaagcga tagcgaagca gaattacgtg
     1441 gcaaccggaa aattgccgca ccttatcgcg gcgcggttgt actggttaat tttgataccg
     1501 atcagcgcaa gccctggttt ataaaagcgt taagagcgga tgggcaacca ttaacgtttg
     1561 gttatgaagt caatgatatc cacggtcata atattggcgt tgtcggccag ggaagccagt
     1621 tatttattcg caccaatgaa ataccgccat cggttaatgt agcaattgat aagcaacaag
     1681 gactttcatg cacaatcacc ttcggtaaag agattgatga aagtagaaat tatatttgcc
     1741 agtaatagtc aggtggttct atggaaattc gcataatgct atttatatta atgatgatgg
     1801 ttatgcctgt gagctatgcg gcatgttata gtgagttatc tgttcagcac aatttggttg
     1861 ttcaggggga ttttgcactt actcaaacac aaatagcgac atatgagcat aattttaatg
     1921 attcgtcatg cgtgagtaca aatactatca cccctatgag ttcgtcggat attattgttg
     1981 ggctttataa cgataccata aaattaaatt tacattttga atggaccaat aaaaacaaca
     2041 tcacgttgtc aaataatcag accagtttca ccagtggtta ttcagttacg gtgacacctg
     2101 cggccagtaa tgcaaaagtg aatgtttctg cggggggcgg cggttcagtg atgattaatg
     2161 gtgttgcgac attatccagt gcttcatcat cgacacgcgg gagtgccgca gtacaatttc
     2221 tactgtgttt attaggtggc aagtcatggg atgcatgtgt aaatagctac agaaatgcat
     2281 tggcacaaaa tgcaggtgtc tattccttta atctgacatt gtcatacaac ccgataacca
     2341 caacctgcaa accggacgat ttattaatta ctttagacag tattcccgtt tcacaattac
     2401 cagccacagg taacaaagca acaataaata gtaaacaagg ggatattatt ctgcgttgta
     2461 aaaatttatt aggtcaacaa aatcaaacat cacggaaaat gcaggtgtat ttatcaagtt
     2521 ctgatttgtt agccggcagt aacacgatcc tgaaaggtgc agaaaataat ggagtcggat
     2581 ttatgcttga aagcaatggt tctccagtca catttttaaa tatcactgat aacagcaaag
     2641 gatacacaaa tttaaaagaa attgcggcaa agtcaaaact tacagataca acggtttcaa
     2701 ttccgataac tgccagttac tacgtctacg atacaaacaa agttaaatct ggcgcactgg
     2761 aggcaaccgc attaatcaac gtgaaatacg actaataaca agcaagacga gcaagtggct
     2821 aataataaaa aataacaagg tgtctgcatt tccccctcat gatgaggggg ctttttttag
     2881 cgatgataaa aaatctcacc gtcgtaggct ttaatgattt taccgtcggt gtcgctgatc
     2941 aacacgtacg cgccacccat ataggtccag tgcgtcccgg catcaggcgc gggcaggtta
     3001 cgtaactgcc actgcttaat gttgtactca tcggtgcggt acatctctgg gacggtatca
     3061 cctggtttga aatgcttaaa gtcagcaatg aaactactta actcatactt agaaattccc
     3121 gaggtattgg tcgaacccgc ggtggctggt gcggcccagg cggcactggt aaccagcaaa
     3181 agcacaccca gcaacatctt atttttaatg gtcataaact ctccactttg gggatctgta
     3241 atatcagtcg catatctttc ctgccataca atataactga caagcgatat tttgttagtg
     3301 atcccatgat ttcgtaaaaa gtgtaaatga aaggatgccc taaaagcatc cttagcgagc
     3361 agagagtcag cggaaatagc tgatttaaga atcatcctgg gctgaagata acatgcaagc
     3421 ctaaaggatt gagttatcgc attatgccca tgaagccatg tacgcccata tacacaccga
     3481 ctaagccaat caacagactg gaaaaatagg gggcgcgttt agcgagagtg ttaaatccgc
     3541 tccagcgttt tgcgacctgc tgaacgctga ttgctgcgcc aacgcctacg gtgacaagcg
     3601 ttaacgccag gccaatgctg aaactgacga ccagtgttgc gcccagtgtc agggctttca
     3661 actgaatgca tatcaacagc acggtaattg ctgccgggca ggggataagg ccaccggtta
     3721 agccaaatag caaaatttgc cagttggtga cctctctacc atcaaagcgt cgtttaatgt
     3781 cattggcatg ggctcgtgca tgggcatcct gatactcgcc atgttcgtga tggtgatgat
     3841 gtccatggtc gtggtgatct tcgtgatcgt gatgatgatg ctcataatca tactcgtgca
     3901 tattctccag ccagttgcgt tcgccgcgcc aggtacgcca gaacatccag aacgcggtgc
     3961 caataatgat cactgcggaa atcagctgga gccacggttc tgctgattga gcagtaaagc
     4021 gcttgctgat caccatcccg ccaaaggcaa ttaaccagac cactgcggta tgcgaaatag
     4081 ttgctgccag tccgagcatc accgcttgtt taatggtgcc tttgatggcg atgataaacg
     4141 ccgccatcat cgtttttgag tgccctggtt ccaggccatg aagcgcacca agtaagatgg
     4201 cgctggggat gaagaaccag gcgtttcctt gctgaagaag agttgtaaat tcggtcatga
     4261 taataattct tagtattaat tcggcaatct gattctactc cccccagtac ctgatactac
     4321 cccccagtag attaatagtg ctatgatatt ttcatattct tgttaaccag gtgttgccat
     4381 gtctcataca atccgtgata aacagaaact gaaagcgcgt gccagtaaga ttcagggcta
     4441 ggtcgtggcg ctcaagaaaa tgctcgacga gccgcatgaa tgcgctgcag ttttacaaca
     4501 gattactgct atccgtggcg cggtaaacgg tctgatgcgg gaagtgatta aaggtcatct
     4561 gacggaacac atcgttcacc agggggatga gctaaaacgt gaagaagatc tggatgtcgt
     4621 tctgaaggtg ctggattcat atatcaaata atttattaac gcgattgtaa aactgccgtt
     4681 tttcctcgtt tacaacgcgt gcgctggaca ttaccatcct cctctgcgat ttatcatcgc
     4741 aaccaaacga ctcggggtgc ccttctgcgt gaaggctgag aaatacccgt atcacctgat
     4801 ctggataatg ccagcgtagg gaagtcacgg accaccaggt cattgcttct tcacgttatg
     4861 gcaggagcaa actatgcaag tcgacctgct gggttcagcg caatctgcgc acgcgttaca
     4921 cctttttcac caacattccc ctcttgtgca ctgcatgacc aatgatgtgg tgcaaacctt
     4981 taccgctaat accttgctgg cgctcggtgc atcgccagcg atggttatcg aaaccgaaga
     5041 ggccagtcag tttgcggcta tcgccagtgc cttgttgatt aacgttggca cactgacgca
     5101 gccacgcgct caggcgatgc gtgctgccgt tgagcaagca aaaagctctc aaacaccctg
     5161 gacgcttgat ccagtagcgg ttggtgcgct cgattatcgc cgccattttt gtcatgaact
     5221 tttatctttt aaaccggcag cgatacgtgg taatgcttcg gaaatcatgg cattagctgg
     5281 cattgctaat ggtggacggg gagtggatac cactgacgcc gcagctaacg cgatacccgc
     5341 tgcacaaaca ctggcacggg aaactggcgc aatcgtcgtg gtcactggcg aggtggatta
     5401 tgttaccgat ggccatcgcg ccgttggcat tcacggtggc gatccgttaa tgaccaaagt
     5461 ggtaggaact ggctgtgcat tatcggcggt tgtcgctgcc tgctgtgcgt taccaggcga
     5521 tacgctggaa aatgtcgcat ctgcctgtca ctggatgaaa caagccggag agcgcgcagt
     5581 cgccagaagc gaggggctag gcagttttgt tccacatttc cttgatgcgc tctggcaatt
     5641 gacgcaggag gtgcaggcat gaaacgaatt aacgctctga cgattgccgg tactgatccg
     5701 agtggtggtg cggggattca ggccgatctt aaaaccttct cggcacttgg cgcttatggt
     5761 tgctcagtta ttactgcact ggtggcgcaa aatacccgtg gcgtacagtc ggtgtatcgc
     5821 attgagcctg attttgtcgc cgcccagctc gattcggtgt tcagcgatgt gcgaatcgat
     5881 accactaaaa tcggtatgtt ggcggaaacc gatattgttg aagcggtggc agaacggttg
     5941 caacgttatc agatccaaaa cgtggtactc gacaccgtta tgctggcaaa aagcggcgac
     6001 ccactgcttt caccttcggc agttgccacg ctacgcagtc gattattgcc acaggtttca
     6061 ttaataacgc caaacttgcc cgaagcagcc gccttgctcg acgcgccaca cgcgcgcacc
     6121 gaacaggaaa tgctggaaca agggcgatcg ctgttggcga tgggctgtgg cgcagtgcta
     6181 atgaaaggtg gtcatctgga tgatgagcaa agcccggact ggctgtttac ccgcgagggt
     6241 gaacaacggt ttaccgcacc gcgcattatg accaaaaaca cccacggtac tggttgtaca
     6301 ctctctgcgg cgttggctgc actacgcccg cgccatacaa actgggctga caccgtacag
     6361 gaggcaaaaa gctggctttc atcggcgtta gcccaggccg acacgctgga agttggtcac
     6421 ggtattggtc cggttcacca cttccacgcc tggtggtgag caccgtacta tagctgcttt
     6481 ggttattgtc agcctttggc actgacattc agggatgaaa ccaatgcagc taagaattac
     6541 cagtcgaaaa aaactcaccg ctctattatg cgctcttggg ctaatttcca tcgttgcaat
     6601 ttatccgcgt cagacggtga attttttcta ttcgacagca gttcagatta cagactacat
     6661 ccacttctac ggttatcgcc cggttaaatc tttcgctatt cgtattcctg ccagttacac
     6721 cattcacgga atagatgttt cgcgctggca ggagcggatc gactggcagc gtgtggcaaa
     6781 aatgcgcgat aacggtattc gcttacaatt tgcctttatt aaggcgacgg aaggcgaaaa
     6841 gctggtggac ccctattttt cgcgtaactg gcaactaagc cgcgaaaatg gcttgctgcg
     6901 cggggcatat cattatttct ccccgtcggt agcagcaccg gttcaggtgc gattatttct
     6961 gcaaacggta gatttctcac aaggcgattt ccctgcggta ctggacgtag aagagcgagg
     7021 aaaattatcg gcaaaagaat tacgcaagag ggtaagtcag tggctaaaaa tggtcgaaaa
     7081 aagtacgggg aaaaagccga ttatttactc aggagccgtt ttttatcaca cgaatctggc
     7141 gggctatttc aatgaatatc cgtggtgggt ggctcactac tatcaacgtc gtccggacaa
     7201 tgacggcatg gcctggcgct tctggcagca ttccgaccgt ggacaggtag atggcatcaa
     7261 tggtccggtg gattttaatg tgtttaatgg cacggtggag gagttgcagg gattcgttga
     7321 tgggattaaa gaaacgcctt aaataagaag cagtgatata ctcccaccag actccctggc
     7381 aggaaaaaga caaatggaac aagcgcatac ccagctgatt gcccaactga acgaacgtat
     7441 tttagcggcg gataacacgc cgctttatat taagtttgcc gaaacggtaa aaaatgccgt
     7501 gcgcagcggg gtgctggagc atggcaatat tttgcccggt gagcgtgatt taagtcagtt
     7561 aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag gcgctggaag aagagggcgt
     7621 ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac atcttcgaat actcgttgaa
     7681 agaagcgcga ggtttttctc agcaggtggt gttgcgcggg aaaaagcccg atacgctatg
     7741 ggttaacaag cgtgtcgtga aatgtcccga agaagtcgcg cagcagctgg cggtcgaagc
     7801 aggaagtgat gtctttttgc ttaagcgtat tcgctatgtc gatgaagaag cggtatcgat
     7861 tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt gatgccatcg ggatttcact
     7921 ttatgactat ttccgcagcc agcacattta cccacaacgt acgcgttccc gcgttagcgc
     7981 ccggatgccg gatgccgagt ttcagtcaca tattcagtta gatagcaaaa taccggtgct
     8041 ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct attgagtaca gcattagcca
     8101 ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag ttcctcgcgc gttggcgcac
     8161 aatcaccgcc ccgatgcccg acaacccacg acgccactgc attgcccagt aatacggcat
     8221 ccgccagtgg cagaccagag gccagcccgg caagtacgcc acctgcatga ctgtcgcccg
     8281 ccccaatggt gtctacaact tgcgttggaa atgccggaat gcagccagaa gcgtcgttgc
     8341 tgaaatacca tgcgccttct ttatcgagac gaacgatcaa cggcgcggca aatttctcct
     8401 gccattgctc gccaagtgtt gttatctctg cggataaagc aaaacgttcg gcggcaatct
     8461 cagcctcttg acgattgagc gacactaaag gtcgacaggc catgatccgc gccagtaatg
     8521 catccgggat atcgccaata cgtgggccaa aatcgataaa cggcgtcacg tcttgcagct
     8581 cttccagcca ttccactaac aattcgccgc agggcgaggc cagttgataa ccggaaaaat
     8641 agagcaggct gccaggcgca acggttaatc gcgccagcca ctggcgattc cactgatttt
     8701 ccacaccgct gaatgacata aaagtgcgtt cgccatccgg ctcaaccagc gccagacacc
     8761 agccgttatc accttcggcg ttatcgatca ggctgattaa gccctctttt gccatccggt
     8821 tgcgaataat ctccgcccac acgccctgac cgagcggcaa ggcattaccc gcttcgatgc
     8881 cgaggcgctt taacgccacg gcaatattca gtgcgcagcc gccaacgtta acgctctgct
     8941 gtttcagttc gatatcgcac ccacgccagg ggagggcata agcgtcggcg atcacgtcaa
     9001 tgaccgccgc gccgacgacc atcaccggct gacgcgtggt taattcaggc agcagcgtgt
     9061 gtaatcgagc gccgctcata ccgcctcccg ttgttgacga tatttcgcca gcgctgtggc
     9121 atagcggttg aaatcaagct gatttaccgc atccagttcc gcctttaacg caggatcgat
     9181 agcgttaacg ccatgcaacg cgccgcaaat tgccgtcgcc atagcaccga tggtgtctgt
     9241 gtcgccgcca aggttagcgc acaggacggc gcagcgattc gggtcggttt gtgctagttc
     9301 aaccagcgca atggcgcacg gaacggactc aatagtgctg gtacctgcgc caacgacctg
     9361 gtaaagctgt tcgctggcgg attcggtgcc gtcggcattg cgcacaattt tcagcgcaat
     9421 ctccagccgc gccgccagtg aggcgctgaa cgtagtgatg cgtttttgct gtgcatgtcg
     9481 cgcaattgaa ggcagggaat caacaatcgc tgaccagctt tctccgtcaa tggcacgaga
     9541 aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga tcggatttat gggtcgggct
     9601 ggacgccagc gctacatcat caataaagga atcaacatca cgcgccggaa gcaaacaacc
     9661 taatggcgag acgcgcatcg ctgcgccgtt ggtcacgccg ttgttttcca gttcagcaac
     9721 gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag gtcggaccta gtacgttttt
     9781 gttaaaggcg tcgaaacgca gcgcccagtc gagaatatta cgcccaatca gatccggatc
     9841 gatcttgcct tcacgttcca gtaacgcatc cgccagacac agcgccatcg aggtatcgtc
     9901 ggtgaattcg gcgcggttaa aataacaggc cgcgttattc tcctttggtc caggaagaaa
     9961 acggtcaatc cagccaaagt gtgctttaac gcggctgcgt ggccaaagct cggagggcat
    10021 ccccatcgca tcccctaacg cctgcccata aagagcaccg agaatacgtt ctgttttcat
    10081 ttaacttccc cttgtgtcaa cgcaatatcg cgatcatcga ccttgatagc cgtaatttcg
    10141 ttgtcggatt cgcggaaaaa aatcatgaac agcacggcga taatggcaat catcaccgcg
    10201 ccgaaagtcc acatcccgga ccagttgaaa gtcagtccgt ttaccggttc ctgataagcg
    10261 aacatctttt ccatcatcac accgccaaga cgatagccga gcaaactgcc gaagccctgg
    10321 cagcagagcg tgatcagccc ctgcgcagcg gtacgcatat gcacgggggc ttttttatcg
    10381 acatagatgt aagcggtaac gtagtaaaaa tcgtaactta cgccgtgaag caaaataccg
    10441 aggaacagta acgcgtaggt gaaatattca tccgtactac cgtaaataaa gaagccatag
    10501 cggatcgcag cggtgaccag accaagcaat aatacctttt tgataccaaa gcgtttagtg
    10561 aaaaacggca atgccagcat aaagaagatt tcagagaact ggccgagcgt catccagccg
    10621 gtggcgtttt tcatgccaac ttcggtcaga taaccgttgg caaagatgta atagaacgcc
    10681 agtggcatcg caaacaggaa tgaacagaag aaaaagacga ggaagttttt atcgcgcagc
    10741 aggatcagcg catccaggcc gagcatgact ttaatatcca ttttgccggt gctttttggt
    10801 ggcgtgtcgg gcaggaaaaa cgcaaacaca ccgagcagag cagaacttcc ggcggtaatc
    10861 agcagcggga tgttagtcgg tgagatatcg gcatacccca gtatttgcgg caagaaacca
    10921 catgccagac cggaggcaat ccagccgata gtgcccatca cacgaatgcg cgggaaatca
    10981 cgctccacat ccggcacgtt ggcaaaagcg atgctgttag tcagcgcaat ggtcggcata
    11041 taggttagag agtaagccag cagtaacgga aagaacccgg caaaagtggt ctgttgcgca
    11101 gcgaaataca tcagcagcgc gcctgcgaac atcaataccg ccagcacttt ttgcgccgag
    11161 aaaaagcggt cagtgatgga gccaaccaga attggcgaca ggatcgccgc aatggcggta
    11221 caggcatacg accagccaat ttctccggca ctaaaaccgc ttttacttaa ccacaaccac
    11281 aatggcacaa accacgcgcc ccagataaac cattcaacaa acatcatgaa cgacagcttt
    11341 gctgttgttt tcatctttta attccttcat gacaggtaat gtaaggtacg cgatgacaat
    11401 accacttaac aatacctttt aaataccttt gcggtgaaaa cttgaccgcg ctaacatttt
    11461 ttctgatgaa tcgagccaac agaaaacgct gaaaaaacat ccaaaagatg gaaaaactcg
    11521 cccgtaacga ccaggattat gattgcccgc aaaaggcggg ccaggacaat ccatagccga
    11581 tatccaatcg gaatttacgg gagcatagta atgacagata ttgcgcagtt gcttggcaaa
    11641 gacgccgaca accttttaca gcaccgttgt atgacaattc cttctgacca gctttatctc
    11701 cccggacatg actacgtaga ccgcgtaatg attgacaata atcgcccgcc agcggtgtta
    11761 cgtaatatgc agacgttgta caacaccggg cgtctggctg gcacaggata tctttctatt
    11821 ctgccggttg accagggcgt tgagcactct gccggagctt catttgctgc taacccgctc
    11881 tactttgacc cgaaaaacat tgttgaactg gcgatcgaag cgggctgtaa ctgtgtggcg
    11941 tcaacttacg gcgtgttggc gtcggtatcg cggcgctatg cgcatcgcat tccatttctc
    12001 gtcaaactta atcacaacga gacgctaagt tacccgaata cctacgatca aacgctgtat
    12061 gccagcgtgg agcaggcctt caacatgggc gcggtggcgg ttggtacgac tatctatttt
    12121 ggctcggaag agtcacgtcg ccagattgaa gaaatttctg cggcctttga acgcgcgcac
    12181 gagctgggca tggtgaccgt gctgtgggcc tatttgcgaa actccgcctt taagaaagat
    12241 ggcgttgatt accatgtttc cgccgacctg accggtcagg caaaccatct ggcggcaacc
    12301 atcggtgcag atatcgtcaa acaaaaaatg gcggaaaata acggcggcta taaagcaatt
    12361 aattacggtt acaccgacga tcgtgtttat agcaaattga ccagcgaaaa cccgattgat
    12421 ctggtgcgtt atcagttagc taactgctat atgggccggg ctgggttgat aaactccggc
    12481 ggtgctgcgg gcggtgaaac tgacctcagt gatgcagtgc gtactgcggt tatcaacaaa
    12541 cgcgctggcg gaatggggct gattcttgga cgtaaagcgt tcaagaaatc gatggctgac
    12601 ggcgtgaaac tgattaacgc cgtgcaggac gtttatctcg atagcaaaat tactattgcc
    12661 tgatacgcct catacgaaca gcaccacccc gtaggacgga taaggcgttt acactgcatc
    12721 cggcaaccta tgcctgatgc gacgcggaag cgtcttatca tgcctacata gcactgccac
    12781 gtatgtcgga aaaggcgttt acaccgcatc cggcataaaa acacgcgcac tttgctacgg
    12841 cttccctatc gggaggccgt ttttttgcct ttcactcctc gaacagtttt catattgtcg
    12901 ttttgtgatc gttatctcga catttaaaaa caaatcattt cattatattt tgaaatcgaa
    12961 aacaagcaac aggatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca
    13021 cagcgcggcg gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg
    13081 gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca
    13141 ttcacttatg ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat
    13201 caccatccac tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag
    13261 gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat
    13321 atttcacggg tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaagcg
    13381 gagctggggc aacttggcgg ccaggaagat gatgtgcaag tcaatgaagc cgatgttttt
    13441 tataccaacc ccgctcaggc gcgtgaattt gccgaggcaa ccggaattga ttccctggcg
    13501 gtcgccatcg gcacggctca tgggatgtat gccagcgcac cggcgcttga tttttctcga
    13561 ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcagggtta
    13621 tcgactaagg atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg
    13681 gagctgaaaa atgccttctc gcaggcgtta aaaaattacc tgaccgagca ccctgaagcg
    13741 accgatcccc gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa
    13801 gtgattgccg attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa
    13861 aaacatgaaa acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt
    13921 ctgttctgcc catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg
    13981 caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat
    14041 gacaccggca gactttcgcg aatttgtttt tacgattgcc gataaagtcg gatttgcacg
    14101 cgaacgcatt attctcggcg gcgatcatct ggggccaaac tgctggcagc aagaaaatgc
    14161 ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag
    14221 taaaattcat cttgatgcgt caatgtcctg cgcgggggat cccataccgt tagcaccaga
    14281 aacggttgcg gaacgagctg ctttgctttg ctgcggaaag tgtggcgaca gattgccagc
    14341 gtgagcaact gagctatgtc attggcactg aagttccggt tccgggcggt gaggccagcg
    14401 ccattcagtc agtacacatc acccatgttg aagatgccgc caatacttta cgtacgcatc
    14461 aaaaggcctt tattgcccgt gggctgacag aggcgttaac acgcgtgatt gccatcgtgg
    14521 tgcagccggg cgtggaattt gatcacagca atattatcca ttatcagccg caggaagcgc
    14581 agccgctggc gcaatggata gaaaacaccc gaatggttta tgaagcacat tctaccgatt
    14641 accagacccg gacggcttat tgggaattag tccgcgatca ctttgcaata ttgaaagtcg
    14701 gtcccgcatt aacctttgct ttacgcgagg cgatatttgc actggcacaa attgagcagg
    14761 aacttatcgc ccctgaaaat cgcagcggtt gcctggcggt aattgaagaa gtgatgctcg
    14821 acgaaccgca atactggaaa aaatattatc gtacgggttt taacgattca ttactggata
    14881 ttcgttacag cctgtcggat cgtattcgtt attactggcc gcatagtcgg attaaaaata
    14941 gcgtcgaaac gatgatggtg aatcttgaag gcgtggacat cccactgggc atgattagtc
    15001 agtatcttcc caaacaattt gaacgcattc agtccgggga attatcagca ataccgcatc
    15061 agctgattat ggataaaatt tatgatgttt tgcgcgccta tcgctacggc tgtgcggaat
    15121 aaggacggta tatgactaac ctgtttgttc gtagcggaat ttcttttgtc gatcgtagcg
    15181 aagttttaac ccatatcggt aatgagatgc tcgccaaagg tgtggttcat gatacctggc
    15241 cacaggcatt aa
//
LOCUS       DAJTYZ010000094        15236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000094 DAJTYZ010000000
VERSION     DAJTYZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.094"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..750
                     /locus_tag="OO596_002866"
     CDS             <1..750
                     /locus_tag="OO596_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR6718671.1"
                     /translation="ESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYE
                     LDKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQE
                     VNAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLL
                     NFDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTAS
                     LQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(791..1723)
                     /locus_tag="OO596_002867"
     CDS             complement(791..1723)
                     /locus_tag="OO596_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6718672.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2087..4873
                     /gene="polA"
                     /locus_tag="OO596_002868"
     CDS             2087..4873
                     /gene="polA"
                     /locus_tag="OO596_002868"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6718673.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5255..5887)
                     /locus_tag="OO596_002869"
     CDS             complement(5255..5887)
                     /locus_tag="OO596_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6718674.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6469..6978
                     /gene="yihI"
                     /locus_tag="OO596_002870"
     CDS             6469..6978
                     /gene="yihI"
                     /locus_tag="OO596_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6718675.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7167..8540
                     /gene="hemN"
                     /locus_tag="OO596_002871"
     CDS             7167..8540
                     /gene="hemN"
                     /locus_tag="OO596_002871"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6718676.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8730..8840)
                     /locus_tag="OO596_002872"
     CDS             complement(8730..8840)
                     /locus_tag="OO596_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6718677.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8952..10361)
                     /gene="glnG"
                     /locus_tag="OO596_002873"
     CDS             complement(8952..10361)
                     /gene="glnG"
                     /locus_tag="OO596_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6718678.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10373..11422)
                     /gene="glnL"
                     /locus_tag="OO596_002874"
     CDS             complement(10373..11422)
                     /gene="glnL"
                     /locus_tag="OO596_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6718679.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11596..13005)
                     /gene="glnA"
                     /locus_tag="OO596_002875"
     CDS             complement(11596..13005)
                     /gene="glnA"
                     /locus_tag="OO596_002875"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6718680.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13378..15201
                     /gene="typA"
                     /locus_tag="OO596_002876"
     CDS             13378..15201
                     /gene="typA"
                     /locus_tag="OO596_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6718681.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3965 a         3803 c         3915 g         3553 t
ORIGIN      
        1 gagtcgaaat ttaatcagat taacattatc aatcacgggg aagacgttgc ccaaatcgat
       61 gctttcgttg caaaaaccat gctggatcgc gttaaagaca aagattatat caatgtcaat
      121 ctgacctacg aacttgataa gttaacaaaa gggaatcagc aactcggtag tggtgagtgg
      181 tcattgattg ctgaatctat tgatccctca gcagtgcgcc aatttatcat ccagtataac
      241 attgctatgc agaagcagct tgctgtacac cctgagttag caaacgatga agttgctctg
      301 caagaagtga atgctgcatt gttcaaagag tatttaccgt tattacaaaa aagtgagccg
      361 accattaaac aaccggtaag atggaagaac gcactcggcg aactaaatgc caatctggat
      421 atcagtattg ccgacccagc caaatcttca tcatccacaa acaaagatat caaattgctc
      481 aattttgatg tgaagttacc gcttaatgtc gtcacagaaa ccgcaaaaca gcttaattta
      541 tctgaaggaa tggatgcgga aaaagcgcaa aagcaggctg ataaacaaat cagcgggatg
      601 atgacattgg gtcagatgtt tcagttaatc acgattgaca acaataccgc ctcgctgcag
      661 ctgcgttata caccgggtaa agttgtgttt aacggacagg agatgagcga agaagaattt
      721 atgtctcgtg ccggacgttt tgttcattaa aatactcact cgcctctgct cattcagagg
      781 cgagtaaaac ttacttattc tgacgctgtg atgacatgag agacgtcaga agccggtctt
      841 tttcctgcca caatgagttc agccattgct gaaaatggcg cttaaagctt ttgtcattga
      901 tgtagtcgcc atgtaactca tcggcaatag gctgtaaatc cacatgtacg acaatccgcg
      961 tcagtttacc gcttaacata tcgaagaacg gctgacggtt attgtccgga taacacagcg
     1021 taacattaag cagtttatcg aattgtttac ccagtacatt tagcgccatc gcaatgcctg
     1081 ccgcctttgg tggcaacaag ttttgaaaag tggagtgggt ttgctgatgt ttttcttgcg
     1141 tgaaacggga gccttcaacg aaattgacaa tggtggtggg atgcaggcga aacttttcac
     1201 aagaacgccg agtggtttca acatctttac cacgtcgctc cggatgacgt aataaataag
     1261 cgcgggaata acgcttcata aacggcatat ccagcgccca gcacgccagg ccaagaaatg
     1321 gcacccaggc cagctgttgc ttgaggaaat atttattcat cggaatgtgc ttacgaaaca
     1381 gcacgcacag tacgacaata tctgcccaac tacggtggtt acaaataagc agataccagt
     1441 tcttcttact taacccttcc agcccgtgaa cttcccattg caggtgtggg ttcagatgca
     1501 gtaataccgc cagaccttca caccagcaat acatcataaa atcacaaaaa cgcgaaacct
     1561 ttcgccaaat gactggcaca ggtagcaata gttttacaat cccggcaatg atgatcggga
     1621 cggaacaaaa aatagtgacc aaaatggtca atacgatact cagcaaaagg gttatcgcag
     1681 cgagtattct cgtcataatg aatttattca aaaggttagc catagacagt gcgctaaaga
     1741 agcgcaaggc gcagatttta gcagaaaacg acccaaataa cggatgatcc ttaaggagaa
     1801 aaataatcca tatctatcca cattagaaaa aatcccatta tctcaattat tagggatggc
     1861 tttattttta actgcatgaa aaacaaagac aaacatcatg ctgtaaaaag catgataata
     1921 aattaaaagc gatgtaaata atttatgcac aaagttatcc acatgacgat ttgcgagcga
     1981 tccagaagat ctacaaaaga ttttcacgaa aagcggtgaa aaactcatgt tttcatcctg
     2041 tctgtggcat cctttaccca taatctgata aacaggcacg gacattatgg ttcagatccc
     2101 ccaaaatcca cttatccttg tagatggttc atcttatctt tatcgcgcat atcacgcgtt
     2161 tcccccgctg actaacagcg caggcgagcc gaccggtgcg atgtatggtg tcctcaacat
     2221 gctgcgcagt ctgatcatgc aatataaacc gacgcatgca gcggtggtct ttgacgccaa
     2281 gggaaaaacc tttcgtgatg aactgtttga acattacaaa tcacatcgcc cgccaatgcc
     2341 ggacgatctg cgagcacaaa tcgaacccct gcacgcgatg gttaaagcga tgggactgcc
     2401 gctgctggcg gtttctggcg tagaagcgga cgacgttatc ggtactctgg cgcgcgaagc
     2461 cgaaaaagcc gggcgtccgg tgctgatcag cactggcgat aaagatatgg cgcagctggt
     2521 gacgccaaat attacgctta tcaacaccat gacgaatacc atcctcggac cggaagaggt
     2581 ggtgaataag tacggcgtgc cgccagaact gatcatcgat ttcctggcgc tgatgggtga
     2641 ctcctccgat aacattcctg gcgtaccggg cgtcggtgaa aaaaccgcgc aggcattgct
     2701 gcaaggtctt ggcgggctgg atacgctgta tgccgagcca gaaaaaattg ctgggttgag
     2761 cttccgtggc gcgaaaacaa tggcagcgaa gctcgagcaa aacaaagaag ttgcttatct
     2821 ctcataccag ctggcgacga ttaaaaccga cgttgaactg gagctgacct gtgaacaact
     2881 ggaagtgcag caaccggcag cggaagagtt gttggggctg ttcaaaaagt atgagttcaa
     2941 acgctggact gctgatgtcg aagcgggcaa atggttacag gccaaagggg caaaaccagc
     3001 cgcgaagccg caggaaacca gtgttgcaga cgaagcgcca gaagtgacgg caacggtgat
     3061 ttcttatgac aactacgtca ccatccttga tgaagaaaca ctgaaagcgt ggattgcgaa
     3121 gctggaaaaa gcgccggtat ttgcatttga taccgaaacc gacagccttg ataacatctc
     3181 tgctaacctg gtcgggcttt cttttgctat cgagccaggc gtagcggcat atattccggt
     3241 tgctcatgat tatcttgatg cgcccgatca aatctctcgc gagcgtgcac tcgagttgct
     3301 aaaaccgctg ctggaagatg aaaaggcgct gaaggtcggg caaaacctga aatacgatcg
     3361 cggtattctg gcgaattacg gcattgagct gcgtgggatt gcgtttgata ccatgctgga
     3421 gtcctacatt ctcaatagcg ttgccgggcg tcacgatatg gacagcctcg cggaacgttg
     3481 gttgaagcac aaaactatca cttttgaaga gattgcgggt aaaggcaaaa atcaactgac
     3541 ctttaaccag attgccctcg aagaggccgg acgttacgcc gccgaagatg cggatgtcac
     3601 cttgcagttg catctgaaaa tgtggccaga tctgcaaaaa cacaaagggc cgttgaacgt
     3661 cttcgagaat atcgaaatgc cgctggtgcc ggtgctttca cgcattgaac gtaacggtgt
     3721 gaagatcgat ccgaaagtgc tgcacaatca ttctgaagag ctcacccttc gtctggctga
     3781 gctggaaaag aaagcgcatg aaattgcagg tgaggaattt aacctttctt ccaccaagca
     3841 gttacaaacc attctgtttg aaaaacaggg cattaaaccg ctgaagaaaa cgccgggtgg
     3901 cgcgccgtca acgtcggaag aggtactgga agaactggcg ctggactatc cgttgccaaa
     3961 agtgattctg gagtatcgtg gtctggcgaa gctgaaatcg acctacaccg acaagctgcc
     4021 gctgatgatc aacccgaaaa ccgggcgtgt gcatacctct tatcaccagg cagtaactgc
     4081 aacgggacgt ttatcgtcaa ccgatcctaa cctgcaaaac attccggtgc gtaacgaaga
     4141 aggtcgtcgt atccgccagg cgtttattgc gccagaggat tatgtgattg tctcagcgga
     4201 ctactcgcag attgaactgc gcattatggc gcatctttcg cgtgacaaag gcttgctgac
     4261 cgcattcgcg gaaggaaaag atatccaccg ggcaacggcg gcagaagtgt ttggtttgcc
     4321 actggaaacc gtcaccagcg agcaacgccg tagcgcgaaa gcgatcaact ttggtctgat
     4381 ttatggcatg agtgctttcg gtctggcgcg gcaattgaac attccacgta aagaagcgca
     4441 gaagtacatg gacctttact tcgaacgcta ccctggcgtg ctggagtata tggaacgcac
     4501 ccgtgctcag gcgaaagagc agggctacgt tgaaacgctg gacggacgcc gtctgtatct
     4561 gccggatatc aaatccagca atggtgctcg tcgtgcagcg gctgaacgtg cagccattaa
     4621 cgcgccaatg cagggaaccg ccgccgacat tatcaaacgg gcgatgattg ccgttgatgc
     4681 gtggttacag gctgagcaac cgcgtgtacg tatgatcatg caggtacacg atgaactggt
     4741 atttgaagtt cataaagatg atgttgatgc cgtcgcgaag cagattcatc aactgatgga
     4801 aaactgtacc cgtctggatg tgccgttgct ggtggaagtg gggagtggcg aaaactggga
     4861 tcaggcgcac taagattcgc ctgaacatgc ccttttttcg taagtaagca acataagctg
     4921 tcacgttttg tgatggctat tagaaattcc tatgcaacaa ctgaaaaaaa attacaaaaa
     4981 gtgctttctg aactgaacaa aaaagagtaa agttagtcgc gtagggtaca gaggtaagat
     5041 gttctatctt tcagaccttt tacttcacgt aatcggattt ggctgaatat tttagccgcc
     5101 ccagtcagta atgactgggg cgttttttat tgggcgaaag aaaagatccg taatgcctga
     5161 tgcgctatgt ttatcaggcc aacggtagaa ttgtaatcta ttgaatttac gggccggata
     5221 cgccacatcc ggcacaagca ttaaggcaag aaaattattc gccgtcctgc gtttcttcta
     5281 caggctgcat ctcgctaaac caggtatcca gtttctgccg cagcttgtcc acgccttgtt
     5341 tcttcaacga agaaaacgtt tcaacctgca catcaccgtt aaacgccagt acagcttcac
     5401 gcaccatatt caattgcgct ttacgtgcgc cgcttgccag tttgtccgct ttggtcagca
     5461 gcaccagaac ggcgatattg ctgtctaccg cccactcaat catctgctga tccaaatctt
     5521 tcagcggatg gcgaatatcc attagcacca ccagaccttg caggctctga cgtttttcga
     5581 ggtattcgcc gagcgcacgc tgccatttgc gcttcatctc ttccgggact tccgcataac
     5641 cgtacccagg caagtcaacc agacgcttgc cgtcagccac ttcaaacagg ttgataagct
     5701 gggtgcgccc tggggttttt gaggtacgag ccaggctttt ctggttagtc agcgtgttca
     5761 gcgcgctgga tttacctgcg ttggaacggc ctgcaaaagc cacttcaatt ccggtatcgg
     5821 aaggtaggtg gcgaatatca ggcgcactca tcacaaaatg cgtctgttga taattcaaat
     5881 tagtcaaagc ggtcgtctcc gtcagtcaaa gctttgtggc gattatacct gaaccataat
     5941 aaaaggcgga tttttcggcg tgagcgttgt aagtaaaagc catacgcttt gtgagacatt
     6001 gccgatagtc tttatgcgaa atagcagaga aaattctgcg atgcatgtca aataagctat
     6061 ataaatcagt gaattgcttt tatataaaac tctgaaaatg tgcaaatact gatggcggtt
     6121 gattgtttgt ttaaagcaaa ggcgtaaagt agcacccata gagcgaggac gctaacagga
     6181 acaatgactc aggatgaggg tcaggagcgc caggaggcga agacagagga ttgtcaggaa
     6241 gacaaacgtc cggagacgta attaaacgga aatggaatca acacggattg ttccggctaa
     6301 aggaaaaaca gggtgtgttg gcggcctgca aggattgtaa gacccgttaa gggttatgag
     6361 tcaggaaaaa aggcgacaga gtaatctgtc gccttttttc tttgcttgct ttctgttaga
     6421 ttccgccgca aatctatact gaataaaacg gctaaaagac gaaccattat gaaaccatca
     6481 tcttcaaact cacgcagcaa aggtcatgca aaagcgcgtc gaaaaacacg cgaggagctg
     6541 gatcaggaag ctcgtgatcg caagcgtcag aaaaaacgtc gtggtcatgc gccgggcagc
     6601 cgcgcagcgg gcggaaacac cacgtcaggc agcaaaggcc agaacgcacc aaaagatcca
     6661 cgtattggca gtaaaacccc tattccattg ggcgtggctg aaaaagtcac caaacagcac
     6721 aaaccgaaga gtgagaaacc tatgctttca ccgcaggcgg agttggaatt actggaaacg
     6781 gatgagcgtc tggatgcgct gctggaacgt ctggaagcag gcgaaaccct gagcgccgaa
     6841 gagcaatcct gggtggatgc taaactggat cgtattgatg agttgatgca gaaactcggc
     6901 ctctcttatg acgatgacga agaagaggaa gaagacgaga agcaagaaga catgatgcgt
     6961 ctgctgcggg gtaactaacg gattgccacc gtgggccttc ccgttctgct gataaccctt
     7021 ccggttatat gttatctggt gtggttattc gttaaactac agcggttgtc gcggcgacaa
     7081 aagtggctgc gcaaccggct gatgacccga aacgggcatc cgccggtacg ccgtagccgc
     7141 cagagacgcc atcggaagga gtgagcatgt ctgtacagca aatcgactgg gatctggccc
     7201 tgatccagaa atataactat tccgggccac gatacacctc gtacccgacc gcgctggagt
     7261 tttcagaaga cttcggcgaa caggcgtttt tacaagccgt ggcgcgctat cctgagcgtc
     7321 cattatctct ctacgtacat atcccgttct gccataagct ttgttacttc tgcggttgca
     7381 ataagattgt tactcgccag cagcacaagg ccgatcagta tctggacgcg ctggagcaag
     7441 aaatcgtcca tcgtgcaccg ctgttcgccg ggcgtcacgt cagccaattg cactggggcg
     7501 gcggaacgcc gacgtatctg aataaagcgc aaatcagccg cctgatgaag ctgctgcgcg
     7561 aaaacttcca gttcaatgcc gatgcggaga tttcgatcga agtcgatccg cgggaaatcg
     7621 aactggatgt actcgatcat ttacgcgccg aaggctttaa tcgcctgagc atgggcgtgc
     7681 aggacttcaa caaagaagtg caacgtctgg ttaaccgcga gcaggatgaa gagttcatct
     7741 ttgcactgct taaccatgcg cgtgagattg gttttacctc caccaacatc gacctgattt
     7801 acggcctgcc gaaacagacg ccggagagtt tcgcctttac cctgaaacgt gtggcggagc
     7861 tgaaccccga tcgtctgagt gtctttaact acgcgcatct gccgaccatt tttgctgctc
     7921 agcgcaaaat caaagatgct gacctgccga gtccgcagca aaaactcgat atcctgcagg
     7981 aaaccatcgc cttcctgacg caatcgggct atcagtttat cggtatggat cactttgccc
     8041 gtccggatga cgagctggcg gtggcccagc gtgaaggcgt gctgcatcgt aacttccagg
     8101 gctacaccac tcagggcgat accgatctgc tggggatggg cgtttccgcc atcagcatga
     8161 ttggcgactg ctacgcgcag aaccagaaag agttgaagca gtactatcag caagtggatg
     8221 aacaaggcaa tgctctgtgg cgtggtattg cgctaacgcg tgatgactgt attcgccgcg
     8281 atgtgattaa gtcgctcatc tgcaacttcc gtctggatta cgcccctatt gagaaacagt
     8341 gggatttgca cttcgctgat tactttgcgg aagatctcaa gctgctcgcc ccgttagcaa
     8401 aagatgggct ggtggatgtg gatgagaagg gaatacaggt gacggcgaaa ggtcgcttgc
     8461 tgatccgcaa catttgcatg tgctttgata cctatctgcg ccagaaagcg cggatgcagc
     8521 agttctcacg ggtgatttaa atagtgaatg gcgcttcgtt tacaaagtag aaaacgaaag
     8581 cgccatcaat gtgctgagaa caagattgcc tgatgcgcta cgcttatcag gcctacaaaa
     8641 cctgttgaaa atattgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
     8701 caaagagcac ttcatcagca atctgaaact caactaaaca gcccaatcat tgcggcacac
     8761 agcacggcag caacggcagt ttgcggtgtg tggctgtcaa ctgcgccact cgataccaga
     8821 ttaattattg attccagcat aatgactctc cccgttttcc gggcaagatc atactgaact
     8881 tatcggaaca gtaaagcgta aaataccagc aatttgcgct caataatcaa tctttacaca
     8941 caagctgtga atcactccat ccccagctct tttaacttac gcgtcagggt gttgcggccc
     9001 cagccgagta gccgcgccgc ttcctgttta tgcccctgcg tatgtcgtaa cgcggtcgtc
     9061 agtaacgtcc gctccagctc tggctgcgct tcggaaagca gattttgatg accggaacgc
     9121 agcgctctgt ctgcccactg cgcaagaagc gttgcccagc tgtccggttg catttgcgaa
     9181 gtactctccg caaccgttga ttcaaacagt tcgccgggca aatcctgaat caacacttcc
     9241 tgcccggcgg ccatcaccgt tagccagcgg caggtgtttt ccagctggcg cacgttgcct
     9301 ggccacgcca gacgcgtcag agcagcttcg gtttccggat gcagtaactt cgcttctacg
     9361 cccagttcgc gcgcggcaac ctgtaaaaaa tggcgcgcca gacggggaat atcttcccga
     9421 cgttcgcgca gcggcggcag atgaacgcgg ataacgttca ggcggtggaa cagatcctca
     9481 cggaacttac cttcctgcac tcgctgttcg agattctggt gagtggcagc gataatacgc
     9541 acatccactt tcaccggcgc atagccgcca acgcggtaaa actgaccgtc tgccagcacg
     9601 cgcagcaaac gcgtctgcac atccagcggc atatcaccaa tttcgtcgag gaataatgta
     9661 ccgccatcgg cctgttcaaa acgcccctga cgaatggtat tcgcgccagt aaacgcgcct
     9721 ttctcgtggc caaacagttc tgattcgatc aaatcttttg ggatagctgc catattcagc
     9781 gcgataaacg gcgctttggc gcgcggactg tggcgatgca gggcatgagc gaccagttct
     9841 ttaccggtgc cggattcgcc gttaatcagc acgctaatag aagaacgcga aagccgaccg
     9901 ataatacgga acacgtcctg catggctggc gcttcgccga tgatatcggt cgttgggccg
     9961 ttaagctgaa tattacgcgg ctgctgctgt tcctggtaat gactgatagc gcgctcaacc
    10021 agcgccactg cttcgtcgat atcaaacggt ttgggcagat aatcaaacgc cccttgttga
    10081 taggcgctga cggcagcatc cagatcggaa tgtgcggtca taatgatgac cggaagcatt
    10141 ggatggcgct gtttaatctg cttgagcagc gccagcccgt ccattcccgg catacggata
    10201 tctgaaagca gcacatccgg cgttttgctc gccagcgcct ccagtacttc cgcgccgttc
    10261 tcaaatgtcg tacaggttaa acccgctcca gcgagcgcac gttcaagcac ccaacggatg
    10321 gaactatcgt catcgactac ccagactatc cctcgttgca taaacgtcac ctttatttcc
    10381 tgataggcag gtaaaccgag aactcggtat gacctggcca actggtaaat tcaattttgc
    10441 ctgaatgctg atcaatcaaa ttacgggcga tggataagcc aagcccggtg ccaccttcgc
    10501 ggccgctgac catcgggtaa aacagcgtat cctgcaaatg aggcggaatg cccggcccgt
    10561 tatcttccac atcaatccgc gccgccagcc ggtagcgctc gccgtgtaag gtcagttgaa
    10621 acgcggtgcg ggtacgcaga atgatttcac cgccttccgg ccccagcgcc tgtagcgcat
    10681 tgcgcacaat attcagcaag acctgttcaa tttgatccgg gtcgtgcgcc agttccggca
    10741 ggctggggtc gtaatcacga atcaaccgca cgttgtccgg cagttccatc gacaccagcg
    10801 tcaccacgcg ttcagctact ttgtgaatac tttcggtaac gcgcgtaccg ggcagctgcg
    10861 gccccaacag acggtcgacc agatttcgca gccggtccgc ctgttcgata atcactttgg
    10921 tatattcgag tagtgatggg tcaggtaacg ctttgctgag cagctgcgcc gcgccacgta
    10981 aacctccaag cggattttta atctcatgtg ccaggccgcg cactaaatca cgggcagcaa
    11041 cctgctgggc gtgctgtagc tgttcctgac ttaagcggcg ctggttatcc atcggagcca
    11101 tctccagcag gatcatgccg tccggcatac gctgggccgt cacagaaagg atatgcgagc
    11161 gcccgtcgat gaccagcgtc acttcgttat cggtaaaacc ttgccccgcc tccagacttt
    11221 cttgcatcag ctcgatattt aatgagaagt agctcaacag ttccggtaac ggtgtaccaa
    11281 acaatttgcg ggagctttgg gcgagcagtt gttgcgcggc ggggttggcg taatggatcg
    11341 ccagattgtc atcaattaac aaaatactgt taatcagcga gttgaggatc tgcccagcat
    11401 cgggctgcgt gcctgttgcc ataaagcagt ctcctgaaca ggttgcacca ttttagtgca
    11461 ttatagcttt ttacggataa aaagcgcgaa gcatcagaga attgacggag aaaaaagccc
    11521 atgcagagat gggctgaaag tttccacggc aactaaatcc cggcgttgtt gcgccgggta
    11581 gtacaacttc aactattaga cgctgtagta cagctcaaac tctaccggat gcggagtcat
    11641 acgcacgcgg tcatcttctt cgcgacgtag agcgatgtac gcatcaattg cttcgtcagt
    11701 gaacacgcca ccggctttca ggaactcgcg gtccagatcc agttcgttca gtgcttcttc
    11761 cagagagcct gcaacctgtg ggatctcttt cgcttcttct ggcggcaggt catacaggtt
    11821 tttgtccatg gcttcgcccg gatggatctt gttcttgata ccatcaagac cggccatcag
    11881 cagggcagca aagcacaggt acgggttagc tgccggatcc gggaaacgta cttcgatacg
    11941 acgtgctttc ggagaagaaa ccaccggaat acggatagac gcagaacggt tacgcgcaga
    12001 gtaagccagc attaccggtg cttcatagcc cgggaccaga cgcttataag agttggtggt
    12061 cgggtttgcc agggcgttaa tcgctttagc gtgtttgatt acgccgccaa tgtagtacag
    12121 cgcctgctca gacagacctg cgtatttgtc gcctgcgaac aggttaacgc cgtttttaga
    12181 cagagacatg tggcagtgca taccggagcc gttatcaccg aacatcggtt ttggcataaa
    12241 ggtcgcggtt ttaccgaagc ggtgcgctac gttgtgcaca acatatttgt agatctgaat
    12301 ttcgtcagct tttttggtca tagtattgaa gcgggtagcc acttcgttct gaccagcagt
    12361 cgctacttcg tggtgatggg cttcaaccac cagacccatc tgttccatca ccagacacat
    12421 ttcagaacga atatcctgag ccgagtctac cggtggaacc gggaagtaac cgcctttcac
    12481 tgccggacgg tgacctttgt taccaccttc gtattgggtg gaggagttcc atgcgccttc
    12541 gatatcgtcg atagcaacgt gggaaccgga gatagatgat ccgaaacgga tgtcatcgaa
    12601 caggaagaat tcaggttctg gcccgaacag tacggtgtcg gcaatgccag tggaacgcag
    12661 gtaatcttcg gcgcgcttcg caatggagcg cgggtcacgg tcatagcctt gcagggtgcc
    12721 aggttcaagg atgtcgcaac ggataatcag ggtggagtcg gcgaagaacg ggtcaatcac
    12781 tgcggtggat gcgtctggca tcagcaccat gtcggactcg ttaatgcctt tccagccgcc
    12841 aatcgaggag ccgtcaaaca ttttgccttc ttcgaagaat tcagcattca cctgatgagc
    12901 agggatagtg acgtgctgtt ctttaccttt agtatcggtg aagcgcaaat caacaaactt
    12961 cacttcgtgc tcgttcagca tcgtcagtac gtgttcagcg gacatacttt aactctcctg
    13021 gattggtcat ggtcgtcgtg gtaacgaaat ctgcaattat tttggccgtg tcgccgtaaa
    13081 aaagataaag cgaaatctgt gccaactttt aaattgcccc taaaaggcgt tatcatgcgc
    13141 accatcgtgc aaaagggctg caccacgatg tgaatgttgc accaatatag tgcttcaatg
    13201 gaaacattaa gcaccatgtt ggtgcaatga cctttggata acccttttta tgctccgtga
    13261 aagcgatcac aaagggactc tgcaatactt gtttgcggag gatgtttgtg atcctgtttt
    13321 gtagtgcgat taatccgtgt acaataacgc gctatttcta atgcctgagg caaagttgtg
    13381 atcgaaaaat tgcgtaatat cgccatcatc gcgcacgtag accatggtaa aaccaccctg
    13441 gtagacaagc tgctccaaca atccggtacg ttcgactctc gtgccgaaac ccaagagcgc
    13501 gtgatggact ccaacgattt ggagaaagag cgtgggatta ccatcctcgc gaaaaacacc
    13561 gctatcaaat ggaatgatta ccgtatcaac atcgttgata ccccggggca cgccgacttc
    13621 ggtggtgaag ttgaacgtgt aatgtccatg gtagactcag tgctgctggt ggttgacgca
    13681 tttgacggcc cgatgccgca aacgcgcttc gtaaccaaaa aagcgtttgc ttacggcctg
    13741 aagccgattg ttgttatcaa caaagttgac cgccctggcg cgcgtcctga ttgggttgtg
    13801 gatcaggtat tcgatctgtt cgttaacctc gacgcgaccg acgagcagct ggacttcccg
    13861 atcgtttacg cttctgcgct gaacggtatc gcgggtctgg accacgaaga tatggcggaa
    13921 gacatgaccc cgctgtacca ggcgattgtt gaccacgttc ctgcgccgga cgttgacctt
    13981 gacggtccgt tccagatgca gatttctcag ctcgattaca acagctatgt tggcgttatc
    14041 ggcattggcc gcatcaagcg cggtaaagtg aagccgaacc agcaggtcac tatcatcgat
    14101 agcgaaggca aaacccgcaa cgcgaaagtc ggtaaagtgc tgggccacct cggtctggaa
    14161 cgtatcgaaa ccgatctggc ggaagctggc gatatcgtgg cgatcacggg ccttggcgaa
    14221 ctgaacattt ctgacaccgt ttgcgacacg caaaacgttg aagcgctgcc ggcactctcc
    14281 gttgatgagc cgaccgtttc tatgttcttc tgcgttaaca cctcgccgtt ctgcggtaaa
    14341 gaaggtaagt tcgtaacgtc tcgtcagatc ctggatcgtc tgaacaaaga actggtacac
    14401 aacgttgcgc tgcgcgtaga agaaaccgaa gacgccgatg cgttccgcgt ttctggtcgt
    14461 ggcgaactgc acctgtctgt tctgatcgaa aacatgcgtc gtgaaggttt cgaactggcg
    14521 gtatcccgtc caaaagtaat cttccgtgaa atcgacggtc gtaaacaaga gccgtatgaa
    14581 aacgtgactc tggacgttga agaacagcat cagggttctg taatgcaggc gctgggcgaa
    14641 cgtaaaggcg acctgaaaaa catgaatcca gacggtaaag gccgcgtacg tctcgactac
    14701 gtgatcccaa gccgtggtct gattggcttc cgttctgagt tcatgaccat gacttccggt
    14761 actggtctgc tgtactccac cttcagccac tacgacgacg tacgtccggg tgaagtgggt
    14821 cagcgtcaga acggcgtact gatctctaac ggtcagggta aagcggtcgc gttcgcactg
    14881 ttcggtctgc aggaccgcgg taagctgttc ctcggtcacg gtgcagaagt ttacgaaggt
    14941 cagattatcg gtattcacag ccgctctaac gacctgactg taaactgcct gaccggtaag
    15001 aaactgacca acatgcgtgc ttccggtact gacgaagctg ttgttctggt tccgcctatc
    15061 cgcatgactc tggaacaagc tctggagttc atcgatgatg acgaactggt agaagtgact
    15121 ccgacctcta tccgtattcg taaacgtcac ctgacggaaa acgatcgtcg tcgcgccaac
    15181 cgcgcaccga aagacgatta atttcgtttt ttagttataa aaaacctgcc atcgct
//
LOCUS       DAJTYZ010000095        15150 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000095 DAJTYZ010000000
VERSION     DAJTYZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15150
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.095"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..316)
                     /locus_tag="OO596_002877"
     CDS             complement(<1..316)
                     /locus_tag="OO596_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6718682.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKW"
     gene            complement(321..506)
                     /locus_tag="OO596_002878"
     CDS             complement(321..506)
                     /locus_tag="OO596_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6718683.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(503..3142)
                     /locus_tag="OO596_002879"
     CDS             complement(503..3142)
                     /locus_tag="OO596_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6718684.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3350..4339
                     /gene="ldhA"
                     /locus_tag="OO596_002880"
     CDS             3350..4339
                     /gene="ldhA"
                     /locus_tag="OO596_002880"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6718685.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4450..4872
                     /gene="hslJ"
                     /locus_tag="OO596_002881"
     CDS             4450..4872
                     /gene="hslJ"
                     /locus_tag="OO596_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6718686.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4869..5135)
                     /locus_tag="OO596_002882"
     CDS             complement(4869..5135)
                     /locus_tag="OO596_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6718687.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5409..8933
                     /gene="nifJ"
                     /locus_tag="OO596_002883"
     CDS             5409..8933
                     /gene="nifJ"
                     /locus_tag="OO596_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6718688.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9299..10432
                     /gene="ompN"
                     /locus_tag="OO596_002884"
     CDS             9299..10432
                     /gene="ompN"
                     /locus_tag="OO596_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR6718689.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10573..11007
                     /gene="uspF"
                     /locus_tag="OO596_002885"
     CDS             10573..11007
                     /gene="uspF"
                     /locus_tag="OO596_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6718690.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11593..12507
                     /gene="sitA"
                     /locus_tag="OO596_002886"
     CDS             11593..12507
                     /gene="sitA"
                     /locus_tag="OO596_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6718691.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12507..13334
                     /gene="sitB"
                     /locus_tag="OO596_002887"
     CDS             12507..13334
                     /gene="sitB"
                     /locus_tag="OO596_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6718692.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13331..14188
                     /gene="sitC"
                     /locus_tag="OO596_002888"
     CDS             13331..14188
                     /gene="sitC"
                     /locus_tag="OO596_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6718693.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14185..15042
                     /gene="sitD"
                     /locus_tag="OO596_002889"
     CDS             14185..15042
                     /gene="sitD"
                     /locus_tag="OO596_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6718694.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         3797 a         3817 c         3668 g         3868 t
ORIGIN      
        1 cccatttacc gtttatccct tgtacatatt cccccggttt ggcccgagct acgagctttt
       61 gcccggcgag tttcgcgata tcatctaccg acacattatt ttgctttgcc agttgttggt
      121 aactcgcttt acgttcggca ttaatgtcgg ttactaattt ctctgtttca gcatccgttt
      181 tcagcgcaac cagataacca taaaatgttt cacctacccg cccctgagtt ctggcttcat
      241 ccagagtcaa tgccattaca ttgctgctta ccagcccaac aagaaacgca caaagaagta
      301 atgttttctt catcatcgcc tcagaaaaga tcgctacgag tttcaagcag ctcttcgaca
      361 tctttgtctg ccttgatgat gatctcatgc tcaattttaa cgttcatatt gatagtgatc
      421 ggttccttgg gcgcagcgac ttcaatacgc ggcgtacagc caaccagcat aaaagatgac
      481 gtcaacgcag ccagtaaaat tttcattgtt tttcctcaca ctcttttcct tgcggacagt
      541 catttcccgg cagacgtgcg ttctgctcca accatgcctg gagattatcg ccaaagcgta
      601 aactgcgcca cagcgtaaaa atattctctt catgatggta attaaggttt accgtaccgc
      661 ttttaccatc aacgcgactg gtacctgtaa tgttggcctg catggttaat aaaccgagat
      721 tatctaagtt aatttttgtc gatgaccggc taatttccat atagcgcaac cagttaattg
      781 ctgaacccgc agtcatattg tctttcacca ccgcatctgc ggtgtcttta tccagccgca
      841 aggtcattgg cccgctattt gccagccaac catctttcac tatccatttt tcgttgttta
      901 gccataacgg caatgcacca ctaaatgccc cggacatggc gaattgtttc ggattgacgg
      961 cgctaaccag ttcgctggat gagagattat tcagccgcaa cagcgccggg tcatgttgcg
     1021 gcatacgtaa ttgtttcatc agtacgttac cgcctaacac atcgacgcta acatcagtca
     1081 acagcaaggg ttcttcttca gtccacggat aacgcccttg caaatcagcc gtaatatttt
     1141 tcgctgtcac cagattaatc acttcggcaa ttcgcaacgt aacggggccg cgagtcccca
     1201 gatgccaggc tccatcggca aaacggaaag gcaggacaaa atcgacaccg ttaacctgat
     1261 tatctggcat ccaggcacta ccgcctttca acacgccgtg tcctcccgcg cggaatcctt
     1321 gttcaggcgc agcagaaaat gcaacctgag catacagttc cccatcgcgt aagttcatct
     1381 tccagtcggg tggcaccagc ggctggaata cagtcagtga ttgttttggc caccaggcgt
     1441 taccgcgcag acgaataccg tcccagcgac catttacccg aaccgggcca atctcaccag
     1501 catgtaaatc gcctttaaag aggaaatacg tcggatcgcg cccatcgacg ctaaatttta
     1561 aggttgatgg cggtaacaca ctgccgccag tgaacagcgt ttgcccggcg tccagtgaca
     1621 gcgcgccgct gaaggaggga tgctgtgcgt cacgtaccca gacgacgggc ttgtcgagaa
     1681 ttaatcgcgg cttttctacc gtcatcgtac cgtattgtaa ctggtcgaaa ccggtggaga
     1741 ggtcggtcag cgtaatcgtg ctgtcatgcc attcaccttt tcctgcgaca tcccaggtgg
     1801 catttatcgg tgtaaaactg ccttttcccc agtagcgcca ctgccagcgg cccgcgtcag
     1861 ggagaaaatc attcgccagc ccatccatat gcagcacgaa atcgcccagt tcatattcat
     1921 gcgcctgcaa aatggcctgc aaacgtccgt caacaccacg ttgggtgact tttacacccg
     1981 ctaaaggcca gcggatttca tcgatgtcca gcgaatcgat gactcttccc ttcgaacgta
     2041 ataacgcgcc tggctcaaag gccagcgttg ggtcagtcag acttccactt agctgcgcgg
     2101 gtaagcgggc atataaaatg aggtccgcct gtttcgcttc accggtcagc tgcagaggca
     2161 gctggctgtt atccatgctt aattttcctg ggccaaaatt tagcaccgcg ttgcctttac
     2221 ccgcttgccc ctgggtcagc acactcagtc gtccgctgat cagagcgttc tcaagccctg
     2281 cctgccagtt gtcgactttg acacccagtc ggccactcaa aggaaaacct gcatacggcc
     2341 aactccagcg accatcgctt acggtcaatt gttgacgagt aatttgccac ggcaaatcga
     2401 gcaacggatc gccgttatcc cgtgccagca caatcaattg cccgctattt tcctgccagt
     2461 ccagctctgc atccaccagt gacggttcct gcggcaagtt taacgtcgca gtagcatgcc
     2521 cacttacagg aagtccatcc ggcacgagcg gcatagtaaa ttcccccacc agtttaaccg
     2581 gcggctgatt ttcaaacgcg atgacatcca gttcgctgac tgtaagttgt tgccctttca
     2641 gctggccttg aaatttaact ttttcgccct gataacgcag ttgctgaata tcagaggtta
     2701 atgcgagaga gagttttccc tgccattcct gccagggaga aaaaatcagt ttatcgatat
     2761 tgatccaggt gttaggcagc atggactgcc actgcgcgag ggtttttgga gcagctggcg
     2821 attgctccgt ctgcggcaat ttcgccagac aagcagaatc aagttctacc atgccgacgt
     2881 tcaataacca gcggctggga tgtgaaaggc tggcgttggt gatatgcgca agctgacaat
     2941 ctcccaccag ataacggaga tcgggaatga ttaaaccttt acgcgtaatg cgtgggcttt
     3001 catctaatgc aatacgtgtc ccgagcggta gccagatgcc cgccagcgtg ggaacccaca
     3061 gcccgagcgt catcagcagc gtcaacggca caagaataat cagtaataac agcgcgagaa
     3121 cggctttata tttacccagc atgggtagtt aatatcctga tttagcgaaa aattaagcat
     3181 tcaatacggg tattgtggca tgtttaaccg ttcagttgaa ggttgcgcct acactaagca
     3241 ttgttgttga tgaatttttc aatatcgcca tagctttcaa ttatatttga aattttgtaa
     3301 aatattttta gtagcttaaa tgtgattcaa catcactgga gaaagtctta tgaaactcgc
     3361 cgtttatagc acaaaacagt acgacaagaa gtacctgcaa caggtgaacg agtcctttgg
     3421 ctttgagctg gaattttttg actttctgct gacggaaaaa accgctaaaa ctgccaatgg
     3481 ctgcgaagcg gtatgtattt tcgtaaacga tgacggcagc cgcccggtgc tggaagagct
     3541 gaaaaagcac ggcgttaaat atatcgccct gcgctgtgcc ggtttcaata acgtcgacct
     3601 tgacgcggca aaagaactgg ggctgaaagt agtccgtgtt ccagcctatg atccagaggc
     3661 cgttgctgaa cacgccatcg gtatgatgat gacgctgaac cgccgtattc accgcgcgta
     3721 tcagcgtacc cgtgacgcta acttctctct ggaaggtctg accggcttta ctatgtatgg
     3781 caaaacggca ggcgttatcg gtaccggtaa aatcggtgtg gcgatgctgc gcattctgaa
     3841 aggttttggt atgcgtctgc tggcgttcga tccgtatcca agtgcagcgg cgctggaact
     3901 cggtgtggag tatgtcgatc tgccaaccct gttctctgaa tcagacgtta tctctctgca
     3961 ctgcccgctg acaccggaaa actaccatct gttgaacgaa gccgccttcg atcagatgaa
     4021 aaatggcgtg atgatcgtca ataccagtcg cggtgcattg attgattctc aggcagcaat
     4081 tgaagcgctg aaaaatcaga aaattgggtc gttgggtatg gacgtctatg agaacgaacg
     4141 cgatctattc tttgaagata aatccaacga cgtgatccag gatgacgtat tccgtcgcct
     4201 gtctgcctgc cacaacgtgc tgtttaccgg gcaccaggca ttcctgacag cagaagctct
     4261 gaccagtatt tctcagacta cgctgcaaaa cttaagcaat ctggaaaaag gcgaaacctg
     4321 cccgaacgaa ctggtttaat cttgccgctc ccctgcattc caggggagct tattcagata
     4381 atccccaaag acctttcatc ttctattctt aaaatagccc tgagtcagaa actgtaattg
     4441 agaaccacaa tgaagaaagt agccgcgttt gttgcgctaa gcctgctgat ggcgggatgt
     4501 gtaagtaatg acaaaattgc tgttacgcca gaacagctac agcatcatcg ctttgtgctg
     4561 gaaagcgtaa acggtaagcc cgtgaccagc gataaaaatc cgccagaaat cagctttggt
     4621 gaaaaaatga tgatttccgg cagcatgtgt aaccgcttta gcggtgaagg caaactgtct
     4681 aatggtgaac tgacagccaa agggctggca atgacccgta tgatgtgcgc taacccgcag
     4741 cttaatgaac tcgataacac cattagcgaa atgctgaaag aaggtgcaca agtggatctg
     4801 accgcgaacc agttaacgct ggcgaccgcg aaacagacat taacttataa gctggcggat
     4861 ttaatgaatt aatagctgcc acagctcccg gcggcaagtg actgttcgct acagcgtttg
     4921 ccgttgggta atgcacacat cccaatcgcc gtaccatcca gttgacgggc aacagaaagc
     4981 gaaccgccga tcattgcaca atttgcttct ccactactgg acatcgacgc ttttaaacct
     5041 ggcgctacgt gcgccgcagt cgcctgctga acaggttcac tactacacgc cgacaacaat
     5101 aaagcggcac accctaccca aaacgctgct cgcatctctt cttcctctga tcttcaagcc
     5161 aaacgacacc gccataaata ataggcagca cagagggcgg cgtcgagagc tgtcctgcgc
     5221 gttgccccgc atttttactt ttttatggct atttttttgc cctctgtttg atcaaaacat
     5281 tcattacgct gatgtggggg acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa
     5341 ttgaaggatt actactgggc gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag
     5401 ggtgtcatat gattactatt gatggtaatg gcgcggttgc ttcggtggcg tttcgcacca
     5461 gtgaagttat cgccatctac cctattaccc ccagttccac gatggcagaa caggctgatg
     5521 cctgggccgg aaacggcttg aagaacgttt ggggagacac accatgcgtg gttgaaatgc
     5581 agtcggaagc gggtgctatc gctaccgtgc atggcgcttt gcagacgggt gccctttcaa
     5641 catcgtttac gtcatcgcag ggtttgctgc tgatgatccc gacactgtac aaactggcag
     5701 gcgaactcac accgtttgtc ctgcatgtag cggcacgtac cgttgccaca catgcactct
     5761 ctatttttgg cgatcattcc gacgtcatgg cggtgcgcca gacgggttgc gcgatgttgt
     5821 gtgcggcaaa cgtccaggaa gcgcaagact ttgctctcat ttcgcacatc gcgacgctga
     5881 aaagccgcgt gccatttatt catttctttg atggtttccg cacgtcccac gaaatcaaca
     5941 aaattgtccc gctggccgat gacacgattc ttgatctcat gccgcaggcc gaaattgatg
     6001 ctcatcgcgc ccgggcgctc aacccggaac atccggtgat ccgtggtacg tccgccaatc
     6061 ctgacactta tttccagtct cgcgaagcca ccaacccctg gtacaacgca gtctatgacc
     6121 atgttgaaca ggcgatgaat gatttctctg ccgcgacagg tcgtcagtat cagccgtttg
     6181 aatattacgg gcatccgcaa gctgaacggg tgattatcct gatgggctca gccattggca
     6241 cctgtgaaga agtggttgat gaattgctaa cccgtggcga aaaagtcggc gtgctgaaag
     6301 ttcgcctgta ccgccccttc tcagccaaac acttgctaca aactctaccg ggatccgtac
     6361 gcagcgtggc ggtactggac agaaccaaag aacccggtgc ccaggcagaa ccgctctatc
     6421 tggatgtgat gaccgcactg gcagaagcct ttaataatgg caagcgcgaa actctgcccc
     6481 gtgtcattgg tgggcgctat ggtctttcat ccaaagaatt tggcccggac tgtgtactgg
     6541 cggtatttgc cgagctcaac gcggctaaac cgaaagcgcg ctttacggtt ggtatttacg
     6601 atgatgtaac caatctgtcc ctgccgttgc cggaaaacac ccagccaaac tcggcgaaac
     6661 ttgaagcctt gttttatggc cttggtagtg atggcagcgt ttccgcgacc aaaaacaata
     6721 tcaagattat cggtaattcc acgccgtggt acgcacaggg ctattttgtt tacgactcca
     6781 aaaaagcggg cggcctgacg gtttctcacc ttcgcgtgag cgaacagccg attcgttccg
     6841 cttatctcat ttcccaggct gattttgttg gctgccacca gttgcagttt atcgataaat
     6901 atcagatggc tgagcgttta aaacctggcg gcattttcct gctcaacacg ccgtacagcg
     6961 cagatgaagt gtggtcgcgc ttgccgcaag aagttcaggc cgtgttaaac cagaaaaaag
     7021 cgcgcttcta tgtgattaac gcggcgaaaa tcgcccgcaa atgtggcctg gcggcccgta
     7081 ttaataccgt catgcagatg gcttttttcc atctgacgca aattctgcct ggcgatagcg
     7141 ccctcgcaga attgcagggt gcgattgcca aaagttacag tagcaaaggc caggatctgg
     7201 tggaacgcaa ctggcaggct ctggcgctgg cgcgtgaatc cgtagaagaa gttccgttgc
     7261 aaccggtaaa tccgcacagc gccaatcgac cgccagtggt ttccgatgcc gcccctgatt
     7321 tcgtgaaaac cgtaaccgca gcgatgctcg cagggcttgg tgatgccctc cccgtttcgg
     7381 cgctgccgcc agacggcacc tggccgatgg gcaccactcg ctgggaaaaa cgcaatatcg
     7441 ccgaagagat ccccatctgg aaagaggaac tctgtaccca atgtaaccac tgcgtcgcag
     7501 cttgcccaca ctcagctatt cgcgcaaaag tggtaccacc cgaagcgatg gaaaacgccc
     7561 ctgccagcct gcattcgctg gatgtgaaat cgcgtgatat gcgcgggcag aaatatgtct
     7621 tgcaggtggc tccggaagat tgcaccggtt gtaacctgtg cgtcgaagtt tgcccggcga
     7681 aagaccgtca gaatccagag attaaagcca tcaatatgat gtcgcgcctg gaacatgtcg
     7741 aagaagagaa aatcaattac gatttcttcc tcaacctgcc agaaatcgac cgtagcaaac
     7801 tggaacgtat tgatattcgt acatcgcagc tgattacacc gctgtttgaa tattccggtg
     7861 cttgctccgg ttgtggcgag acgccgtata ttaaattgct gactcagctc tatggcgacc
     7921 ggatgttgat cgctaacgcc accggctgtt cttcaattta tggcggtaac ctgccctcta
     7981 caccgtatac caccgatgcc aacggtcgtg ggccggcatg ggcgaactct ctatttgaag
     8041 ataatgccga atttggcctt ggtttccgcc tgacggtcga tcaacaccgt gtccgcgtgc
     8101 tgcgtctgct ggatcaattt gccgataaaa tcccggcgga attactgacg gcgttgaaat
     8161 cagacgctac gccagaggtt cgtcgagaac aggtcgcagc tttacgccag caactcaacg
     8221 atgttgccga agcacatgaa ctgctacgtg atgcagatgc actggtggaa aaatcaatct
     8281 ggctgattgg tggtgatggc tgggcttacg atatcggctt tggcggtctg gatcatgtat
     8341 tgagtttgac ggaaaacgtc aacattctgg tgctggatac gcaatgctat tccaacaccg
     8401 gtggtcaggc gtcgaaagcg acaccgctgg gtgcagtaac taaatttggc gagcacggca
     8461 aacgtaaagc gcgtaaagat cttggcgtca gtatgatgat gtacggtcat gtttatgtgg
     8521 cgcagatttc tctcggcgcg cagctgaacc agacggtgaa agcgattcag gaagcggaag
     8581 cgtatccggg accatcgctg atcattgctt atagcccgtg tgaagagcat ggttacgatc
     8641 tggcactcag ccatgaccag atgcgccaac tcacagctac cggcttctgg ccgctatatc
     8701 gcttcgatcc tcgtcgtgcc gatgaaggca aactgccgct ggcgctggat tcacgcccgc
     8761 cgtcagaagc actggaagaa acgttacttc acgagcaacg tttccgtcgt ctgaattcgc
     8821 agcagccaga agtggcagaa cagttatgga aagatgctgc agctgatttg caaaaacgct
     8881 atgacttcct ggcacaaatg gccggaaaag cggaaaaaag caacaccgat taaatgctct
     8941 ggataaggat tacccaattc taaaaaaaag cccggacgac tgttcgggct tgtcttttta
     9001 tatgttggaa aatcagtggc aatgcaatgg cccaacagaa aataaaatct gtgacaataa
     9061 aggcatataa cccgcgcaga ataacgtata gaaaattaaa agtataattt ttattttgta
     9121 tgtattatct tcgatacatt caccagactt attatcattt cagaagaatt atttgattaa
     9181 ggttttactt aaggcgtaac aaatgatatt tatcggctaa ctgaacttct cctttaggat
     9241 gttttcactc cccggaatgg ggataaaagg caaataaaat aactaaagga tttattcaat
     9301 gaaaagcaaa gtactggcac ttttaattcc tgccctgctc gccgcaggtg ctgcacatgc
     9361 agccgaagtt tataataaag acggcaacaa attagatctg tatggcaaag ttgatggcct
     9421 gcattatttt tctgataatt cagcgaaaga tggcgatcag agctatgcgc gtctgggttt
     9481 taaaggcgaa acccaaatta acgatcaact cactggctac ggtcaatggg aatacaatat
     9541 tcaggcaaac aacactgaat cttcaaaaaa ccagtcatgg acccgtctgg catttgccgg
     9601 gctgaaattt gcagattacg gttctttcga ttacggacgt aattatggcg taatgtacga
     9661 catcgaaggc tggaccgata tgctgcctga atttggcggt gactcttata ccaatgcaga
     9721 caactttatg actggtcgtg ccaacggcgt cgcgacttat cgtaatactg atttcttcgg
     9781 tctggtaaat ggtctgaact tcgcggtgca gtatcaaggt aacaacgaag gtgccagtaa
     9841 tggtcaggaa ggcaccaaca acggacgtga tgttcgccat gaaaatggtg acggctgggg
     9901 tctttccaca acatatgatt taggcatggg ctttagcgct ggtgcggcat acacctcatc
     9961 tgaccgcacc aatgaccagg ttaaccatac tgcggcgggt ggtgataaag cagacgcgtg
    10021 gactgctggg ctaaaatacg atgctaacaa tatttacctg gcaaccatgt attcagaaac
    10081 gcgtaatatg accccgtttg gcgacagcga ttatgctgtt gcaaacaaaa cccagaactt
    10141 tgaagtcact gcacagtacc agtttgattt tggtctgcgt ccggcagtct ctttcctgat
    10201 gtctaaaggc cgtgacctgc acgctgccga tggtgcagac aacccggcag gtgttgatga
    10261 taaagatctt gttaaatacg ccgatgttgg cgcgacttac tatttcaata aaaacatgtc
    10321 cacttatgtt gactataaaa tcaacctgtt ggatgaagat gacagtttct acgctgccaa
    10381 cggcatctct accgatgata ttgtcgcttt aggtctggtt tatcagttct aaatcctcct
    10441 gcccgctgtt atggcgggct ttttctgctt attcttcctc ctttgatcta aattaaaaat
    10501 gtgaactccg tcattacaca aaaagtgtca tctggcgtta cactttatgc ggatactaaa
    10561 acaggaggtt ttatgaacag aacgattctt gtccctatcg atatttccga ttcagaatta
    10621 actcaacgcg tgattagcca cgttgaggca gaggcaaaga ttgacgatgc agaggttcat
    10681 ttcctgacgg taataccttc actgccttac tatgcctctc tgggtttagc gtattccgca
    10741 gaattaccgg caatggatga cctgaaagcg gaagccaaat cgcaactgga agagatcatt
    10801 aaaaaattta aactgccaac cgacagagtg catgtccatg ttgaggaagg ctcgcccaaa
    10861 gaccgcattc tggaattggc gaagaagatc cccgctcata tgatcatcat tgcttcccat
    10921 cgaccggata tcaccactta tctgctcggt tccaacgccg cagctgtagt gcgtcacgca
    10981 gagtgctccg tgctggttgt gcgctgacac taacgcccgc acattgctgc gggctttttg
    11041 attcatttcg caaatgtgct gacattttcc cctctaatcc gtaccataca cgccacagtt
    11101 ttatatcaga tttcttatgc tgggcgttcc ggcatgacgc atactcttct gatgccatat
    11161 aacgaattga gtcgctttta aatgtcgcaa aatcaagaaa ttagtaagaa agaacaatac
    11221 aacctgaaca agtaagggca aaaatcacaa ctatctgacc cataagtatt ttatttattt
    11281 ttcaatatgt taaacattat cagcgacaac aataagctac gataacccac tctttttttg
    11341 cccctttttt gccccttcta cagcgttttg ccccattttt gccatcaaca attttcctca
    11401 taaagtcatt tcaaagccta atagctgctc tacttttttc ctcaatatca tgtcgccagc
    11461 aggaaacaac ctaatccctg ttttaggcct caaagctgtt aatgataatc attttcattt
    11521 tgtatgggtt atgctataaa acatagccgt tgctatgttt gttctaattt tgctcactat
    11581 aggtactaaa ttatgcactc aataaaaaaa gtaaccatgc tcttggggtg cctcgccctc
    11641 acctgctcga tcgcatttca ggcaagtgca actgaaaaat tcaaggtcat tacaacattt
    11701 accatcatcg cggatatggc caaaaacgtg gctggagatg ctgcagaagt ctcatccata
    11761 accaagcctg gtgcagaaat tcatgagtat cagcctaccc ctggcgatat taaacgtgcg
    11821 cagggggcac aactgattct cgccaatggt atgaatctgg aattgtggtt ccaacgcttt
    11881 taccagcatc tcaatggggt tccagaagta attgtctctt cgggtgtgac gccagtaggg
    11941 atcaccgaag gaccctatga gggcaaacct aatccccatg catggatgtc gccagataat
    12001 gctctgattt acgtcgataa tattcgtgat gcgttgataa aatacgatcc ggcaaatgca
    12061 caaacctacc aacgtaatgc cgatacttat aaagccaaga ttacccaaac ccttgccccc
    12121 ctgcgtaagc agattacgga actccctgag aatcagcgat ggatggtcac cagtgaaggg
    12181 gctttttctt atctcgcacg cgatttgggg ctaaaagagc tttatctgtg gccgattaat
    12241 gccgatcaac aaggaacacc gcagcaggta cgtaaggttg ttgatatagt taagaaaaat
    12301 catatcccgg cagtctttag cgagagtacg atttccgata aaccagcgcg tcaggttgcg
    12361 cgtgaaaccg gcgcacacta cggcggtgtg ctctatgtcg attccctgag cacagaaaac
    12421 ggcccggtac ctacgtatat cgaccttctt aaggttacta ccagtacgct ggtacaggga
    12481 attaaagccg ggaaaaggga gaaataatga tgcaatctgc aggcattgtc gttaatgatg
    12541 ttactgtcac ctggcgtaac gggcacacag cactgcgcga tgcatccttc acagtaccaa
    12601 gtggatctat tgccgctctg gttggggtaa acggttccgg aaaatcgacg ttgtttaaag
    12661 cgattatggg atttgtgcgt ctgaccagcg gaaagatatc tgttctgggt attcccacgc
    12721 gacaggcgct acagaaaaac ctggttgcct acgtccctca gtcagaagag gttgactggt
    12781 catttcctgt actggtggaa gatgtggtga tgatgggccg ctatgggcat atgggtatgt
    12841 tgcgtatagc caaaaagagg gatcgtcaga ttgtcaccga tgcgctggaa cgtgtcgata
    12901 tggtggattt tcgccatcgt caaatcggcg agctttccgg tggacaaaag aaacgcgtct
    12961 ttctggcgag agcgattgca cagcagggtg atgtgatcct gctggatgaa ccttttaccg
    13021 gcgttgatgt gaaaaccgaa gcgaaaatca tcagcctgtt gagggagtta cgcgcggaag
    13081 gcaaaacgat gcttgtctca acgcacaatc tcggctcagt cacgacattt tgcgattata
    13141 cggtcatggt caaaggcacc gtcctggcaa gcgggccgac agacaccact tttacagccg
    13201 agaacctgga gctggctttt agcggcgtac tgcgccatgt cacacttaac gactcggaag
    13261 aaagcattat tactgacgat gaacgccctt ttgtggcaca tcgaccgtca gcggtgcaga
    13321 gggaagcaag atgaacgtgc tactggaacc cttcagctat gaatatatgc tcaatgcgat
    13381 gtgggtctcg gcaatggtcg gcggtctctg cgctttcctg tcatgctatc tgatgctaaa
    13441 aggctggtcg ctgattggcg atgcgctgtc gcactctatt gtcccgggtg ttgcgggggc
    13501 atatatgctg gggcttccgt tttctctggg agccttcttt tccggcggac tggcagcggg
    13561 cagcatgctg tttcttaacc aacgcacccg cttaaaagaa gatgccatca tcggcctgat
    13621 cttctcgtcc ttttttggcc tggggctgtt tatggtgtca ttgaacccga catccgtgaa
    13681 cattcagacc atcgtcctgg gcaatattct ggcaatcgat ccggcggata tcctgcaact
    13741 cactatcatt ggcattctct cgatcatcgt gctttttttc aaatggaaag atcttatggt
    13801 gacctttttc gacgagaatc acgcccgtgc tattggatta catccgggca gattaaaaat
    13861 cctcttcttc accctgctgt cggtttcgac cgttgcggcg ctgcaaacgg ttggtgcctt
    13921 tctggtgatc tgtctggtgg tgactcccgg tgcaacagca tggttgttaa ccgatcgttt
    13981 tccccgcctg ctcataattg ctgtaactat tggcagtgta accagtttcc tcggcgcgtg
    14041 ggtcagttac tttctggacg gcgcgacagg tggaatcatc gtggtggcgc agacactgct
    14101 gtttctttta gcatttgtat ttgcgcccac ccatggactt ttggcaaatc gtcggcgtgc
    14161 gcataaagcg ctggaggatc gctcatgatg gcattattac tggagccact gcagttcacc
    14221 tttatgagtc acgcactgct gatttcactg gtagtttcca taccctgtgc gctgctgtcg
    14281 gtctttctgg tcctgaaagg ttgggcactg atgggtgacg cgatgagtca tgcggttttt
    14341 cctggcatcg tgcttacctg gattctgggg ttacctctgg ctacaggggc attcgtcgcc
    14401 ggagtattct gcgcggtggc gacgggatac ctgaaagaca acagccgaat caagcaagat
    14461 acagttatgg ggattgtttt ttctggcatg tttgccgcag gtctgatctt gtatattgca
    14521 atcaaaccag acgtacatct tgaccatatt cttttcggcg acatgctcgg gataaccatc
    14581 ggcgatataa tccagacggt gattattgcc gggctggtta cacttgttat tagcgcaaaa
    14641 tggcgggatt ttttgctgtt cagctttgat tatcaacagg cgcaggtaag cggtttgcat
    14701 acgagatggc tgcattatgg gctgctgtgc atggtttccc tgaccattgt ggcgacgctg
    14761 aaagcagtag gtatcattct ctctatttct ctgctcattg cgcctggcgc cattgcagta
    14821 ctcctgacac aacgctttca tattgcgctc ctactggcga ctggcatatc ggtaatagtg
    14881 tcaatgactg gtgtctggct gtcctttttt atcgacagcg caccagctcc gacgattgtc
    14941 gtcttattcg cggtcgtgtt catcatgacg tttaccgtta ccagcatcaa cgcacgcaca
    15001 aagggaaacg cccatacaca ggatctgtta tcacccaatt agcacaacat ccgaggacag
    15061 catcgtccgc cagcctacag aagtccggaa agccatggaa ggtcgttggg aggaggtacg
    15121 aattctatca tgcaaaaaat acgtaaaatc
//
LOCUS       DAJTYZ010000096        15095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000096 DAJTYZ010000000
VERSION     DAJTYZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15095
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.096"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..761
                     /locus_tag="OO596_002890"
     CDS             <1..761
                     /locus_tag="OO596_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6718695.1"
                     /translation="TDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQQT
                     KNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRTIH
                     LVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGPHY
                     KVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFN
                     VYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            797..1423
                     /gene="mlrA"
                     /locus_tag="OO596_002891"
     CDS             797..1423
                     /gene="mlrA"
                     /locus_tag="OO596_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR6718696.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSA"
     gene            1543..1650
                     /locus_tag="OO596_002892"
     CDS             1543..1650
                     /locus_tag="OO596_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6718697.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1631..2362)
                     /gene="yehW"
                     /locus_tag="OO596_002893"
     CDS             complement(1631..2362)
                     /gene="yehW"
                     /locus_tag="OO596_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6718698.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2367..3293)
                     /gene="yehX"
                     /locus_tag="OO596_002894"
     CDS             complement(2367..3293)
                     /gene="yehX"
                     /locus_tag="OO596_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6718699.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3286..4443)
                     /gene="yehY"
                     /locus_tag="OO596_002895"
     CDS             complement(3286..4443)
                     /gene="yehY"
                     /locus_tag="OO596_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6718700.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4450..5367)
                     /gene="osmF"
                     /locus_tag="OO596_002896"
     CDS             complement(4450..5367)
                     /gene="osmF"
                     /locus_tag="OO596_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6718701.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5619..7916)
                     /gene="bglX"
                     /locus_tag="OO596_002897"
     CDS             complement(5619..7916)
                     /gene="bglX"
                     /locus_tag="OO596_002897"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6718702.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8112..9827
                     /gene="dld"
                     /locus_tag="OO596_002898"
     CDS             8112..9827
                     /gene="dld"
                     /locus_tag="OO596_002898"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6718703.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9866..10798)
                     /gene="pbpG"
                     /locus_tag="OO596_002899"
     CDS             complement(9866..10798)
                     /gene="pbpG"
                     /locus_tag="OO596_002899"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6718704.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10972..11559)
                     /locus_tag="OO596_002900"
     CDS             complement(10972..11559)
                     /locus_tag="OO596_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6718705.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11729..12307
                     /locus_tag="OO596_002901"
     CDS             11729..12307
                     /locus_tag="OO596_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6718706.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12437..13198)
                     /locus_tag="OO596_002902"
     CDS             complement(12437..13198)
                     /locus_tag="OO596_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6718707.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13251..14687)
                     /gene="mdtQ"
                     /locus_tag="OO596_002903"
     CDS             complement(13251..14687)
                     /gene="mdtQ"
                     /locus_tag="OO596_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR6718708.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            14911..14994
                     /gene="yohP"
                     /locus_tag="OO596_002904"
     CDS             14911..14994
                     /gene="yohP"
                     /locus_tag="OO596_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6718709.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3659 a         4133 c         3788 g         3515 t
ORIGIN      
        1 caacagataa tggttataca tcaggaaatc catttaacgg tctgacacca ttaaaaaagt
       61 ccaagataga accagatccc ctcacccgtg acgaatttat tcgttttatt gaggcttgcc
      121 gtcatcaaca aacaaaaaac ctgtggatta tcgctgtata cacgggtatt cgtcacgggg
      181 agctggtatc actggcctgg gaagatatag accttaaagc gaggactata accatccgcc
      241 ggaattatac aaaactaggc gaattcactc caccaaaaac tgatgctggt accggaagaa
      301 cgattcatct agttcaacca gctattgatg ctcttaaaag ccaagcggaa atgaccatgc
      361 ttggaaaaca gcattctgta gaggtaaagc agagggaata tgggagaagt actgtgcata
      421 aatgcacttt tgtttttagt cctcaggtaa taaaacagcg gcagttttcc ggaccgcact
      481 ataaggttga ctccatcagg gagtcatgga caagtatctt aaaacgcgca ggtctgagac
      541 acagaaaatc gtaccaatcc aggcatactt atgcatgctg gtcacttgcc gctggagcta
      601 atcctagttt tatcgcaagc cagatgggcc acacaaacgc acaaatggta ttcaatgttt
      661 acggagcatg gatgaaagac aacaatcacg aacagataga actccttaac aaaagactat
      721 ctgaaagtgt cccatgtatg ccccataaga aagtggggta aaataaaaac ttgtaaaatc
      781 agttagttta cccttaatcc ctgtcacgtt acgcgcgtgg cagaggcgtt acgggttgct
      841 gaaaccgcaa cggacagacg gcggtcatcg actgttcaac gatgccgata ttgaccggat
      901 ccgcgagatc aaacgctgga tcgacaacgg cgtgcaggtc agcaaagtta aaatgctgct
      961 cagtaatgaa aatgttgatg tgcagaacgg ctggcgcgat cagcaagaaa cattactgac
     1021 ctacctgcaa agcggcaatc tgcatagcct gcgaacgtgg atcaaagagc gcggtcagga
     1081 ttaccccgcc cagacactca ccacacatct gtttattcct ctgcgccgac ggcttcagtg
     1141 ccaacaaccg actctccagg cgctgctggc gatcctcgac ggcgtactga tcaactacat
     1201 cgccatttgt ctggcttcgg cacgtaaaaa acagggtaaa gatgcgctgg tggttggctg
     1261 gaatattcag gataccaccc gtctgtggct ggagggctgg attgccagtc aacaaggatg
     1321 gcgcattgat gtcctcgccc actcgctcaa tcaactacgc cctgaactat tcgaaggccg
     1381 tacattgctg gtgtggtgcg gtgaaaatcg aacctccgcc taacagcagc aactcaccag
     1441 ttggcaagaa caaggccatg atattttccc actcggcatt taatgattcg ttaacaaatg
     1501 cgctttactg tacaatcctt tcgttaacat aaggagtgca atatgcgcat agctaaaatt
     1561 ggggtcatcg ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt catgctcgca
     1621 ggttatacct ttattttgcg tgctggctaa gcgcctgcac cagcctttca aacaggcggt
     1681 ctgcgatgat cgccgccagt gccaccagta acgccccctg gatcacatac gcggtattaa
     1741 atccgctaag cccgatgatg atgggcgtac ccagcgtgct ggcccctacc gttgaggcga
     1801 tcgtcgccgt accaatgttg ataatcaccg aagttcgcac gcccgccaga atcaccggag
     1861 ccgccagcgg tagctcgacc ttacgcagtc gctgaccacg actcattccc atacctttcg
     1921 caacttctgt cacgctggca tcaatcgctc ccagcccggc aagtgtcgcc tgcaggacgg
     1981 gcagcacacc gtaaaggatc aaggcgataa tcgctggttg cagaccaaag ccgatcaccg
     2041 gaacggcaat cgccagcact gcgacaggcg gaaaagtctg tccaacggcg gcaatagttt
     2101 ccaccagtgg gcgaaattcc gcgccccacg ggcgagtgac agcaattccg gcaccagtgc
     2161 caatgatcac cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag
     2221 ctgcaaaact ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca
     2281 gcggctggct gtaaggcagc cagaaaatca gtgccacaaa caaagcaatg agccagaaca
     2341 gcggatcgcg caacatcttc atacgcttac gcctcctcca gcagatcctg aaaatgcagc
     2401 gtgccgcaag gctggccctg cgtgttcacc accggcagca cctcgcatcc ccgcgcgaca
     2461 aacagggaga gcgcatcgcg tagcgtcatc tcttctgcca gtgcctcacc ttctgcccgt
     2521 tcttcgcgac gcacgtaatc cgccacacta cgtaacgaaa gcaggcgcac acccagttcg
     2581 ctgcgcccaa aaaactggcg gacaaaatcg tttgccggac gagtcagcat cgtcagcgga
     2641 ttgccctgct gcaccacttc accgtgatcc atcaatacca gatgttctgc cagccgtagc
     2701 gcctcatcaa tatcatgagt gaccagcaca atggtacgcc ccagcaaacg gtgaatgcgc
     2761 gtcatctctt gttgcaacgc gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc
     2821 atcagtaaga cttgcggatc ggcagccagt gcgcgcgcca ctcccacacg ttgctgctga
     2881 ccaccggaaa gctgatgcgg ataacgctca cgtaaatttg actccagccc cagtagcgcc
     2941 attaattcgt cgatacgatc gtcaatcctc gcccgcgacc atttttgtaa ttgcggcacg
     3001 gtagcgatgt tttgcgccac gctccagtgg gggaacaggc caatagattg aatggcatag
     3061 cccatccggc ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg
     3121 atcactccgc tgtcatgctc caccaggcgg ttaatcattt tcagggtggt ggatttgccg
     3181 gagccagatg tgccaatcag caccgaaaaa ctcccttcct gaaaattgag attgagatcg
     3241 ttaacggctt tttgtgcgcc gaacagtttg ctgacatggc caaattcaat cattacgttt
     3301 caccttcagc agtgcgataa gcaaatcgaa cagcgcgtcg gtaagcaccg ccagaacaat
     3361 taccgggatc acccccagca acactaaatc aatggcgctg cttagcagcc cctggaaaac
     3421 cagcgcacca aaaccgcctg cgccgattaa cgccgcaatc accgccatac ctacagtttg
     3481 caccatcacc acccgcaggc tgcgcagaaa taccggtaac gccagtggta actgaacatg
     3541 caggaatcgc tgcgccccgc tcatgcccat cgctctggcg ctctccagca catcgcgcgg
     3601 gatctggttc aaaccgacta ccacgccgcg caccagcggc agcaaggcat agagcaccag
     3661 cgcaatcagt gcgggtgtca ttccggttcc tgctatgccg agcgtcccca gccacggaaa
     3721 ggccgtaacc agcgcggcaa gcggcgcaat caacaggcca aagagcgcca ccgaaggcac
     3781 ggtctgaatg acattgagca gagaaaaaat tgccccctgc cgagcagtgg aaaagtagca
     3841 ccagatgccc aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt
     3901 cagatgttgt gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattag
     3961 tgagagatcg ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat
     4021 atgcaacaac cagcgccaca gcggatgcgt ggagattcgg cggatggcat cactacaggc
     4081 cagcaatgcc agcgccgcgg ccagccagaa accactgccg aggctggtac gcgccagcgc
     4141 actgccattt tgcgccagtt gggtcgccgc ctttcctgct ccccacacca gcaatacgaa
     4201 gacgaattgc gccagaatga gtgcaaaaat gctgcctttt ttagcgggaa taaaacaggc
     4261 tgtcagccag gcgcaaccaa cgcccaccag catccagatc gtttgcggcc acagttgcca
     4321 gagatgacgc ccttcacccg agactaaacg attaggcgcg taactgataa acggcagcgc
     4381 cgctgcgatt gccgtcagca acagcagcag cgccagaaca ggattaatac gtagataagt
     4441 cacgggaaat tacttcgtcc accctttttg tttcaggtag tcggcagcca cttttttggc
     4501 atccagtcct tccactgcaa tgctggcatt cagttgctgc aatgtttttg catcgaggct
     4561 ggcgaagact ggctgtagcc actgtgccat ttgcggatac tctttcagca ccgactcacg
     4621 caccactggt gcaggcgcgt agataggttg cacgcctttc ggatcgctta aggtttgcag
     4681 ccccagcgcc gcgaccggac cgtcagtgcc gtaagccatt gcggcattaa cgccagaagt
     4741 ttgctgggca gcggctttaa tcgttaccgc cgtgtcgccg ccagccagtg acagtaactg
     4801 atcctgatcg agtttaaagt cgtaggcttt ttcaaacgcg ggtaacgcat cggcgcgttc
     4861 gataaactct gccgaggctg ccagtttgaa ggtgccgccc tctttcagat aacgactcag
     4921 gtcggcaagc gaagtgagtt tgtttttctc tgccacatcc tgacgcacgg cgatggtcca
     4981 ggtgttattc gctggtgcgg gcgtcagcca gattaacttg ttttgctctg catcgagttt
     5041 tttgactttc tcgtaacctt gctgcgcgtt tttccatgcc gcatcgtttt catctttaaa
     5101 gaagaaagcg ccattaccgg tatattccgg atagatatcc agttcaccgg aagtaatcgc
     5161 cccgcgcacc acaggagtcg ttccgagttg cactttattc accgtcggta cgccgtggct
     5221 ttccagcacc tgcaagatga tgttgccgag tagcgcgcct tcggtatcga tttttgaacc
     5281 gactttaacg ggggaagccg cctgtagcgg caggctcacg gctgccaaca taaccagtga
     5341 acctgcccag acctttgaga gtggcatgat gctttcctca ttcttttact gttgttttca
     5401 gcgaattaag agaaaagcat agttgataat gacagagtta gcctgagcgg aaggattcag
     5461 ttgcagaatc agataaatac ttaaagggca tattttgccc ggcgtttctt gccggatgcg
     5521 gcgtaaacgc cttatccggc ctacaaaggt cgaaaattca gcaagttaag atatgaacat
     5581 caggcaaagg gcgcaaacgt cgcgccctta ctaaagcatt acagcaactc aaactcgcct
     5641 ttcttaacgc gtgcggaatc agtgccgata aagacattga acttgccagg ttcggcgtca
     5701 tatttcatct gttgattcca gaacttcagc gcctcgatat caatcgggaa gctgacagtc
     5761 tgagtttcgc ccggtttcag ggtgattttc tcaaagcctt tcagctgctt cactggacgg
     5821 ctcatggaag ccgtcacatc ctgcaagtac atctgtacta ccgtggcccc ttcgcgttta
     5881 ccggtgttcg tcacctgcac gctggcggtc actttgccgt cacgcttcat ggtcggcgca
     5941 gaaagtttca catcagagac ggtgaaagtg gtatagctca gaccatagcc gaacggataa
     6001 agcgccccgt tagcttcatc aaaataacgc gaagtgtatt tgttcggctt gtcggcatta
     6061 tacggacgac cggtattcag atggctgtag tacaccggga tctgcccgac agaacgcggg
     6121 aaggacattg gcagcttgcc ggacgggttg taatcgccaa acaatacatc ggcaattgca
     6181 ttaccgcctt cagtccccgc aaaccaggtt tccagaatcg catcagcctg ctgatcttct
     6241 ttcaccagcg ccagcggacg cccgttcatc agcaccagca ccagcggttt accggtggct
     6301 ttcagcgccg taatcaagtc acgttggctt tgcggaatag tgatatcggt acggctggag
     6361 gcctcgtgcg ccatcccctg tgcttcaccg accacagcca ccaccacatc agattgtttc
     6421 gccgtctgca ccgcttcatc aatcatctct tgcggcgagc gcggatcgac tttgaccgct
     6481 tcttcatact gattcaggaa atcgataatg cctttgtcac tggtaacgtt cgcccctttg
     6541 gcatacagca ctttaccgtt ttcaccgacg gcatttttaa tcccggtcag tacggtcacg
     6601 gattgatcgg caacaccggc tgcggaccag ctgcccatca cgtcacgttt actgtccgcc
     6661 agtggcccaa ccaccgcaat ggtggccgat tttttcagcg gtaacgtttc gagacggttt
     6721 ttcagcaaca ccaggctttc gcgcgccact tcacgcgctt ctttacggtg caggcggctt
     6781 tcggcattgg tatccaccgg gtcagactct ttcggcccca aatggctgta tgggtcgtta
     6841 aacaatccca tatcatattt aacgttcagt acatggcggg cagcatcgtc cagctcttcc
     6901 atcgtcactt tgccggattt gatcaaccca ggcagatact tcgagtaata ctcgtcgctc
     6961 atactcatgt tgattccgga tttcagcgcc acgcgcaccg catcttccgg gtctgccgcc
     7021 gtgccatgtt taatcagctc tttgattgca ccgtgatcgg aaacggtgat gcctttaaag
     7081 ccccactggt cgcgcagaac atctttcagc agccaggaat cggaggtggc tggcgtgccg
     7141 ttcagcgagt tcagcgccac catcaccgcg ccgctgcctg cgtccagccc cgctttgtac
     7201 ggcggcatat aatcattaaa caggcgctgc ggactcatat cgacggtgtt gtactcttta
     7261 ccgccttcta ccgcgccgta tgcggcaaag tgtttgacgc tggtcatcac cgagtagcga
     7321 tctgccgggc ttttaccctg catcgcttcc accatggttt tacccattgt tgaggtgaga
     7381 tacgtatctt cgccaaaacc ttcggaagca cgtccccagc gcggatcgcg cgagacatcg
     7441 accatcggtg cccaggtcat attcaggcca tcatctgccg cttcataagc agagacacgt
     7501 ccgaccgttt tcactgcatc gaggttaaaa gacgaggcca gaccgaggct aatcgggaac
     7561 accgtgcgct gaccgtgcag cacgtcgtaa gcaaagaaaa gaggaatttt caggcggctt
     7621 aattccatca cctgatcctg catggcgcgg atatcctgac gggttacggt gttgaaaatc
     7681 gccccaacct gaccgtcttt gatcatctcg cggatcgcct ctttcgggtt atccggaccg
     7741 acgctgatta agcgcagctg accaattttc tcatcaactg tcattttctt aagcagttcg
     7801 gtgacgaacg catcccgcgc ttcgggcgtt aatggatggt tgccgaataa atcatccgcc
     7861 agtgctggct gcagggccag actcaccgcg attcctactg aacatagcca tttcatatgg
     7921 atttttcttc ctctgttgcc cgacgaggca gcgaaaatgg tgcaaaaacc gtagtttgcc
     7981 ataagcatga tggagagaga aaaagaatgc tcagtttatt gtctgaattt tcaaaatatt
     8041 cactcgctga attgtcatac aaggcgctat tctagtttgt gatatttttt cgccaccaca
     8101 aggagtggaa aatgtcttcc atgacaacaa ctgataataa agcctttttg aatgaacttg
     8161 cccgtctggt cggtcattca cacctgctca ccgatcccgc aaaaacggcc cgctatcgca
     8221 agggcttccg ttctggtcag ggcgacgcgc tggctgtcgt tttccctggc tcactactgg
     8281 aattgtggcg ggtgctgaaa gcctgtgtca ccgccgacaa aattattctg atgcaggctg
     8341 ccaatacagg cctgaccgaa ggatcgacgc caaacggtaa cgactatgat cgcgatatcg
     8401 tgatcatcag caccctgcgt ctcgacaagc tgcacgttct tggcaagggc gaacaggtgc
     8461 tggcctatcc gggcaccacg ctctattcgc tggaaaaagc cctcaaaccg ctgggacgcg
     8521 aaccgcactc agtgattgga tcatcgtgta taggcgcatc ggtcatcggc ggtatttgta
     8581 acaactccgg cggctcgctg gtgcaacgtg gcccggcgta taccgaaatg tcgttattcg
     8641 cgcgtataaa tgaagacggc aaactgacgc tggtgaacca tctggggatt gatctgggcg
     8701 aaacgccgga gcagatcctt agcaagctgg atgatgatcg catcaaagat gacgatgtgc
     8761 gtcacgatgg tcgtcacgcc cacgattatg actatgtcca ccgcgttcgt gatattgaag
     8821 ccgacacgcc cgcacgttat aacgccgatc ctgatcggtt atttgaatct tctggttgcg
     8881 ccgggaagct ggcggtcttt gctgtacgtc tagatacctt cgaagcggaa aaaaatcagc
     8941 aggtgtttta tatcggcacc aaccagccgg aagtgctgac cgaaatccgc cgtcatattc
     9001 tggctaactt cgaaaatctg ccggttgccg gggaatatat acaccgggat atctacgata
     9061 ttgcggaaaa atacggcaaa gacaccttcc tgatgattga taagttaggc accgacaaga
     9121 tgccgttctt ctttaatctc aagggacgca ccgatgcgat gctggagaaa gtgaaattct
     9181 tccgtccgca ttttactgac cgtgcgatgc aaaaattcgg tcacctgttc cccagccatt
     9241 taccgccgcg catgaaaaac tggcgcgata aatacgagca tcatctgctg ttaaaaatgg
     9301 cgggcgatgg cgtgggcgaa gccaaatcgt ggctggtgga ttatttcaaa caggccgaag
     9361 gcgatttctt tgtctgtacg ccggaggaag gcagcaaagc gtttttacac cgtttcgccg
     9421 ctgcgggcgc agcaattcgt tatcaggcgg tgcattccga tgaagtcgaa gacattctgg
     9481 cgttggatat cgctctgcgg cgtaacgaca ccgagtggta tgagcattta ccgccggaga
     9541 tcgacagcca gctggtgcac aagctctatt acggccattt tatgtgctat gtcttccatc
     9601 aggattacat agtgaaaaaa ggcgtggatg tgcatgcgtt gaaagaacag atgctggaac
     9661 tgctacagca gcgtggcgcg caataccctg ccgagcataa cgtcggtcat ttgtataaag
     9721 caccggagac gttgcagaag ttttatcgcg agaacgatcc gaccaacagt atgaatccgg
     9781 ggatcggtaa aaccagtaag cggaaaaact ggcaggaagt ggagtaaaaa ttacggatgg
     9841 catagtatcg ccatccgtca ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca
     9901 tttgggcggc tttttgtttt ttatagctca acgctgctgc tggaacaggc atcactttac
     9961 cagtttcaat ccaggtacgc aggcggctgg catcggcaaa atgggtatat ttgccaaacg
    10021 catccatcac taccagcgct accggtttat tattgataac cgtacgcatc accagacaat
    10081 ggcccgccgc attggtaaaa ccggttttgg ttaactgaat attccagtta tcgcgataca
    10141 ccagatggtt agtattgcgg aacggcagcg tatacgtcgg gttagagaag gttgccatat
    10201 cttcccgggt agtacttaac tgcccgatca acggatattg tttgctggca atgagcagtt
    10261 tggttaagtc acgggcagtt gaaacgttat gcaccgacaa tccggtaggt tcaacaaagc
    10321 gcgtgttgtt cattccgagc gatttcgctt tcgcattcat tgccttaata aaggctttgt
    10381 aaccaccggg ataatggtgt gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg
    10441 ccagcaacag catatcttta cggctgattt cgctattcag tcgtacgcgc gaatagaccc
    10501 ctttcatctc cggcgtctgg ctgatatcca cttttagttt ttcatccagc ggcagtcgtg
    10561 catccagcac aaccatcgcg gtcattaatt tgctgataga cgcaatcgga cgcaccagat
    10621 ccgggtggtt cgagtagatc actttgttgg tattcagatc aacaatcatc gcgctaccgg
    10681 aggcaatttc aggttgtgaa gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg
    10741 ccaaaggcac agccagcatc agggccaggc taaataaaga aactcgaaat ttcggcatga
    10801 tgagcattca gatagtggtt cacgcgcaca ggttgcgcac cgccggagta aggatttact
    10861 gaggctagcg acgccatcat aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagg
    10921 aaatgctgca ttgctgacat ttacgccagc aatgcaacgt caaagaactt tctagaacaa
    10981 acgataaccg tagccccata gtataacggt tagggcgagc agcacttcca gtaccagcac
    11041 gccaatcgcc agcgtcgaac tagagaagct aaggccttcc tctttgttga tattcaggaa
    11101 gctcggaata ccaaggtaaa gcaggtagcc ggtgtaaaac agcgccaccg tgccgaccag
    11161 cgcacacaac cagaccagtg gataaagcgc caccagaccg cttaaaaaca gcggagttgc
    11221 aacgtagccc gcgaagacca tgcagtgcgc aagtgacgga cgctgcggat aattacgcgc
    11281 catccaccag atgacccgcc ccattaccgc aaccccagcc agcataacgc cataaaacag
    11341 gacagccagc gccagtccgg taaaccagga taacttcagg atagtgccat cgccaaaatt
    11401 ccagccaatc tgtgtagtgc caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa
    11461 aacgtggtgg gtgtaatggt gagaaatcgt ttcgttttcg cgattaatca cctgcatttc
    11521 acgatcggga tgggaaaaca gtccccagac atggctcata ccgccccctt gttgtgagtt
    11581 catgaacctg acagttcaag tataagtcag cttgtgatta ttttttgttc gctcatgtaa
    11641 tttgcaccgt ctgatgtacg tcctttttca gcgtgaatga tttccagggt gtatgcttac
    11701 aggcaaccaa agggagacag actggcctat ggatctcaat acactcatct cacaatatgg
    11761 ttatgccgcg ctggtgatcg gtagcctggc ggaaggtgaa accgtgactt tgctgggagg
    11821 cgttgcggca catcaggggc tattaaagtt cccgctggtg gtactttctg tggcgcttgg
    11881 cggcatgatt ggcgaccagg tgctttatct gtgcgggcgg cggtttggcg gcaagctgtt
    11941 acgccgtttc tcgaaacatc aggataaaat tgagcgggcg cagaaactta tccaacgcca
    12001 tccgtatctg tttgtcattg gtacgcgctt tatgtatggc tttcgggtga ttggcccgac
    12061 gctgattggt gccagccagc tgccgccgaa aatctttctg ccgctgaata ttctcggcgc
    12121 atttgcctgg gcgttgattt ttaccactat tggttacgct ggtggtcagg tgattgcgcc
    12181 gtggttgcac aatctcgacc agcatttgaa gcactgggtc tggttgattc tggtcgtggt
    12241 tctggtggtg ggcgtgcgct ggtggctgaa acggcgcggg aagaaaaagc cggatcatca
    12301 ggcgtaaaac cattgccctg ggtaaggcgt tcgcgccgca tccgacatcc ggtgcaccat
    12361 gcctgatgcg acgcttgtgc gtcttatcat gcctaccaaa attttaccca ttgaaataac
    12421 cacgcacctc actgcgctat tctgggttga actgcggatt cgccaacata aagccgccat
    12481 ccactatcaa cgactgcccg gtggtgtaat ttgcgccctc tgagcacagc cacgctacca
    12541 ggctggcaat ctcatgcgtt gcgccaaaac gccgcagagg aatcgaaggc tccgcgtcgg
    12601 gcttcacgtc gctgtcatcc atgccattca ttggcgtggc gatcgcccca ggcgcgactg
    12661 cgttcaccaa aatcttatgc ctgaccagct ccagcgccat cgttttggtt aacccgccga
    12721 gcgcatgttt agcggctgtg taagcgctgg catccggcag cggcgtatgt tcatgtaccg
    12781 acgtaatgtt gatgatacga ccgccctgcc cttgtttcac catctggcgg gccgcaattt
    12841 gtgagcataa aaatgcacca tcgacatcaa cggtaaaaat cttgcgccac tcatcgaaag
    12901 ccatatcaag aaacggcgct ttggtcattg cgcccgcatt attcaccagc acatcaatac
    12961 gccccagccg ttgaatgagt ttctccagcg cctgcgcacc ttctggcagt ttaccgagct
    13021 ccagctgcac gatctccgca cgtacgccgt ggctaactac ctcacgcgcg gtatcttttg
    13081 ccccttcttc atctgagtgc caggtaatgc caatatcaaa cccctgctgc gccagtaata
    13141 acgcgcactc tttgccgatc cccgaatcgg aggcggtaat aatcgcaacc tgtgccatca
    13201 agttctccac ttaacgctga ataaacgtta agtatagaag gcgcatatca tcagcgtttg
    13261 taacccccgc ccaacgcacc agtgagttga atagaagcat ccagccactg cccttgcaat
    13321 aacaggccat tggcccgctc acgcagcgcg gggattctgg cttcgctgac gcgggaacca
    13381 acaatgatgc ccgcgttaaa gcgcgcctgc gcaagaccta ccacacgcaa ggcatcgcgc
    13441 tcaatttgcg cctgatgctg gtttttctcc gcaagtgtct gaacctgact ggctgcccgc
    13501 gccacgtcat tcaccgcttc aaccaccgct ttgttgtagc tggcgataga caagttgctt
    13561 tcggcttttg cgatattgag attggcgtta agacgaccac tatcgaaaat gggtagcgtc
    13621 aggcctgccg taacgcccat ttgctgcgcg gaatgacgga acagatcgct taagtgcaac
    13681 gcatcctgtt gcaggaaggc catcaggttg atgtcaggat aaaatgccgc ttttgccgca
    13741 tcaatggtgc ttagcgatga ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg
    13801 gccagtaagg agtaccccag ttcatcagga agctggcttg ccactttcgg caacgcgacc
    13861 ggtttaagct tcaatgactt tgtctggtta tttgtaagtg cgcttaaccg tgcctcaata
    13921 attttcattt tccccgcgac atcgttgagc tgctgccggg ttttgctggc attaatatcg
    13981 gtttccacac cttcaactga agaagtaatc ccgttctgat atagctggcg atcggtcgcg
    14041 ataatggtgt tctgctcttt ttctatttgc tgcaagaccg tgtttaacgc cgcctgggtt
    14101 tgccactccc agtacaggcg ggctacgctg ccagccagca attggcgggt ttgctcgcgt
    14161 tccgccgccc gtgctttaac cgtacccagg cgggcagtaa cctccgcccg attctttccc
    14221 cagatatcga gatgccagcc cgccgttaag ccaaaagtac cgttggtgta ccacgggccg
    14281 gtcgtacctg cggccggatc gttcagagca aacggcccca ttaagccttc tgccgacatt
    14341 ttttgccgct ccatatccgc cgaaaagtcg atctgcggac catcctgagt ggcaactgcc
    14401 ttcgcctggg cttcagctag ctgaatgcgc tgttcagcca cctgcatatc cggtgcgttc
    14461 tgtagtgcat tgttaattaa tgaagtgagc tgattatcgt gatactccag ccaccattgg
    14521 ctgtctggcc aaccattttt cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcgggc
    14581 gtttgctggc ttaacgcctg gcgggtttca tgcataggcg cacacccggc cagcatcagt
    14641 aacagcggaa aacaggcgat ggctggataa aaggaatcac gattcatggg ggaataatca
    14701 ggtaagaaac ggtgcgcgga gattaccgtg tgttgcgata tattttttag tttcgcgtgg
    14761 caatacatca gtagcaataa aacgacatac ccagaaaaat atagactaag taaatgatat
    14821 cttccgattt atcttaatcg tttatggata acggcaaagg gcttcgtttt ttcctatact
    14881 tattcagcac tcacaaataa aggaacgcca atgaaaatta tactctgggc tgtattgatt
    14941 attttcctga ttgggctact ggtggtgact ggcgtattta agatgatatt ttaaaattaa
    15001 ttaatgtcat caggtccgaa aataacgaga atatttcagt ctctcatcct gttgcgctcc
    15061 tgtcatgtgc attgcttcat ataatcactg gcgca
//
LOCUS       DAJTYZ010000097        15086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000097 DAJTYZ010000000
VERSION     DAJTYZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.097"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..781
                     /locus_tag="OO596_002905"
     CDS             <1..781
                     /locus_tag="OO596_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718710.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="OO596_002906"
     CDS             complement(984..1337)
                     /locus_tag="OO596_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6718711.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OO596_002907"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OO596_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6718712.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="OO596_002908"
     CDS             complement(3165..3458)
                     /locus_tag="OO596_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6718713.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="OO596_002909"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="OO596_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6718714.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OO596_002910"
     CDS             complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OO596_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6718715.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="OO596_002911"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="OO596_002911"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6718716.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="OO596_002912"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="OO596_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6718717.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="OO596_002913"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="OO596_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6718718.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="OO596_002914"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="OO596_002914"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6718719.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="OO596_002915"
     CDS             10838..11413
                     /locus_tag="OO596_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6718720.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="OO596_002916"
     tRNA            11520..11595
                     /locus_tag="OO596_002916"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            12212..13749
                     /gene="cadC"
                     /locus_tag="OO596_002917"
                     /pseudo
     CDS             12212..13749
                     /gene="cadC"
                     /locus_tag="OO596_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            14114..>15086
                     /gene="cadB"
                     /locus_tag="OO596_002918"
     CDS             14114..>15086
                     /gene="cadB"
                     /locus_tag="OO596_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR6718721.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3765 c         3617 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgctgc
//
LOCUS       DAJTYZ010000098        14965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000098 DAJTYZ010000000
VERSION     DAJTYZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.098"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="OO596_002919"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="OO596_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6718722.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="OO596_002920"
     CDS             complement(1608..1931)
                     /locus_tag="OO596_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6718723.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OO596_002921"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OO596_002921"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6718724.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OO596_002922"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OO596_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6718725.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OO596_002923"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OO596_002923"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6718726.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OO596_002924"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OO596_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6718727.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="OO596_002925"
     CDS             7531..8568
                     /locus_tag="OO596_002925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6718728.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OO596_002926"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OO596_002926"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6718729.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGKTPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="OO596_002927"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="OO596_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6718730.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OO596_002928"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OO596_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6718731.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="OO596_002929"
     CDS             10739..11620
                     /locus_tag="OO596_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6718732.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OO596_002930"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OO596_002930"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6718733.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="OO596_002931"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="OO596_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6718734.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="OO596_002932"
     CDS             complement(14017..>14965)
                     /locus_tag="OO596_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCR6718735.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3783 c         3922 g         3648 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt cttgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAJTYZ010000099        14624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000099 DAJTYZ010000000
VERSION     DAJTYZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.099"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1035)
                     /gene="modC"
                     /locus_tag="OO596_002933"
     CDS             complement(<1..1035)
                     /gene="modC"
                     /locus_tag="OO596_002933"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6718736.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTLLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQI"
     gene            complement(1038..1727)
                     /gene="modB"
                     /locus_tag="OO596_002934"
     CDS             complement(1038..1727)
                     /gene="modB"
                     /locus_tag="OO596_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6718737.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(1727..2500)
                     /gene="modA"
                     /locus_tag="OO596_002935"
     CDS             complement(1727..2500)
                     /gene="modA"
                     /locus_tag="OO596_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6718738.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(2666..2815)
                     /gene="acrZ"
                     /locus_tag="OO596_002936"
     CDS             complement(2666..2815)
                     /gene="acrZ"
                     /locus_tag="OO596_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6718739.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            2944..3732
                     /gene="modE"
                     /locus_tag="OO596_002937"
     CDS             2944..3732
                     /gene="modE"
                     /locus_tag="OO596_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6718740.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            3800..5272
                     /gene="modF"
                     /locus_tag="OO596_002938"
     CDS             3800..5272
                     /gene="modF"
                     /locus_tag="OO596_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6718741.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            5533..6549
                     /gene="galE"
                     /locus_tag="OO596_002939"
     CDS             5533..6549
                     /gene="galE"
                     /locus_tag="OO596_002939"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6718742.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            6559..7605
                     /gene="galT"
                     /locus_tag="OO596_002940"
     CDS             6559..7605
                     /gene="galT"
                     /locus_tag="OO596_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6718743.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            7609..8757
                     /gene="galK"
                     /locus_tag="OO596_002941"
     CDS             7609..8757
                     /gene="galK"
                     /locus_tag="OO596_002941"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6718744.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            8751..9791
                     /gene="galM"
                     /locus_tag="OO596_002942"
     CDS             8751..9791
                     /gene="galM"
                     /locus_tag="OO596_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6718745.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            9994..10746
                     /gene="gpmA"
                     /locus_tag="OO596_002943"
     CDS             9994..10746
                     /gene="gpmA"
                     /locus_tag="OO596_002943"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6718746.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(10905..11957)
                     /gene="aroG"
                     /locus_tag="OO596_002944"
     CDS             complement(10905..11957)
                     /gene="aroG"
                     /locus_tag="OO596_002944"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6718747.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            12273..12653
                     /locus_tag="OO596_002945"
     CDS             12273..12653
                     /locus_tag="OO596_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6718748.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEKHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            12767..13708
                     /gene="zitB"
                     /locus_tag="OO596_002946"
     CDS             12767..13708
                     /gene="zitB"
                     /locus_tag="OO596_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6718749.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13705..14424)
                     /gene="pnuC"
                     /locus_tag="OO596_002947"
     CDS             complement(13705..14424)
                     /gene="pnuC"
                     /locus_tag="OO596_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6718750.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(14462..>14624)
                     /gene="nadA"
                     /locus_tag="OO596_002948"
     CDS             complement(14462..>14624)
                     /gene="nadA"
                     /locus_tag="OO596_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6718751.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3673 a         3879 c         3668 g         3404 t
ORIGIN      
        1 aatttgcgcg tacagccaca ggccaggttt gatcgccagt tcatccctgg cccacgggct
       61 gatacgcgcc cacagcgttt taccgccgac ttccagctcc acttccacct ggccgttgtc
      121 gtcataacta ttaacaactt ttgcccgcaa tacgttacga atgctggttt gctgcggcgg
      181 ttgtaaaacc agagaaacat cggaagcctg aatgcggata cgtagcgcag cttgcagcgg
      241 ttcgtccagc ttattgaccc acaaatgctg atcgcccagc gccagcgcgg tcatcgcgta
      301 atgcggatga tgctcaagta gcgtcacttt cagaatgcta ctttgttgct ctttcggcag
      361 ccacggattc atcacgctac tgccccacac ttcctccagc gcgccaaagg ctttcacctg
      421 accgttttcc agtaccatca ctctgtctgc cagatggagg atctcatcca gcgaatggct
      481 gacatacaac atcggaatgt tgatttcccg tgtcagccgt tgcagataag gcaacagttc
      541 gcgtttacgc ggaatatcca gtgacgccag cggttcatcc agcaacagca attccggtgc
      601 tgtcagcaaa gcccgaccaa tcgccacgcg ctgtttttcg cctccggaca ggctgcctgg
      661 taaacggtca agcaacggtt caatgcctaa aagcgccacc agcttatcga actgatcgac
      721 catacttttc gacatgccgt agcgcagatt gccacgcact ttgtaatgcg ggaacagccg
      781 cgcatcctga aaaacatagc caacgcgacg cttttccggc gtcaggcaga tacctttttc
      841 ggcatcattt agtacccgcc cattgaggac aatccgccct ttttgcgggc gcgtcagtcc
      901 actgatggcg ttaatcagcg aagtttttcc ggcaccggag acgccaaaga tagcagtgat
      961 gccattggcg ggcagtgttt cattaatggt caggcaatgg ttgcccaacg tctgggaaaa
     1021 attcagctcc agcatgatta gcgccccgcc cgttcacggc tgattctggc cagccattct
     1081 gaaatcaaca gggagatcat cgccagcgca atagaaataa tgcacagtct cgccgcgcca
     1141 ctttccccgc cgggggtctg gatcagggta tacatggcag agggaatggt tcgcgtttcg
     1201 ccaggaatgt tcgacacaaa ggtgatggtt gcaccaaact caccgagaga acgagcaaaa
     1261 gccagtaccg taccaacaat aattcccggt aaggtcagcg gtaacgtgat agtaaagaaa
     1321 acgcgccagc gcccggcacc tagtgttctt gcggcctgtt cgagtttgac atcaacccct
     1381 tccagcgcca gacgaattgc ccgcaccatc agcggaaacg acatgacggc ggcagcgaga
     1441 accgcgccgc gccagctaaa ggcgaaggta ataccaaacc agtcatacag acgttcaccg
     1501 ataaatccgc gccgtcccat cgaaactaat aataagtaac cgacgaccac gggcggtaac
     1561 accagcggta gatgcagtac gctgtcgagc agagctttgc ccggaaacgt gcaacgcacc
     1621 agtaaccagg caaaaaagat cccaaacggc aggctaaaca gcacagccag ggaagaaact
     1681 ttcaggctta ataaaactgc ctgccattct ggatcggtca gtatcattac ttggttgtaa
     1741 atccgtaacg tttaaagatt tccgctgcct gcggtccctt cagataatca taaaatgctt
     1801 tcactgtcgc attgttatgc ccttccacaa cagcaaccgg atattccact tttttatgtg
     1861 aatcttccgg gaaggtggca accactttta cccctttgct gacaactgcg tcagaaccgt
     1921 aaacaatgcc caacggcgct tcattgcgtt cgaccaacgc cagcgcgcca cgaacatctt
     1981 ccgccggagc cagtttcggg gagagcgtat cccatgcgcc cagtttttgc agtgcttctt
     2041 ttgcataaat gccagcaggg acatgttccg gatcgccaac cgcgaggcga ccgccattca
     2101 gcagcgaagt ccagttagtt gtgctgtcga tgatgaaatc tttctgctcg ctggctttcg
     2161 gtgctacaac gaccaggcta ttgccgagca gtgtctggcg cgaagccgta tcgatcgctt
     2221 ttttatcaac cgcataatcc atccatttct gatcggcaga gataaacaga tccgcaggcg
     2281 ctcccgcttc aatctgacgg gccagagtag atgacgaagc gaaagaagaa accacatcca
     2341 cgcctttttc ttttttatac tgcgtagcaa tgtcctgcat tgcgttagtc agtgatgcgg
     2401 cggcgaacac cgtgattttc ccttcatctg ccagtgtatt gccagcaaca gcgaaagaga
     2461 gtgccgcccc ggcaaacaag ttcaaccatt tacgagccat tggtaacccc ttaatgtaac
     2521 gttatatagg tgacaatgta acggctagct cagaattttc ccagcagtta ttgctaacct
     2581 ttgtgaggta gataagaaaa aatatcggct ggaaaagcag gaagttgaga aaaagaaatg
     2641 cccgactaag cgggcattca gggaatcaat gattttgtcc gggctggtct tttttaccaa
     2701 caccagaaaa gatgttgaat acttcaccaa gaccgtaaat cagacccagg atgatggcca
     2761 tcacgacagg taccatgatt acggcgaata ccagactttt taataactct aacatggtca
     2821 actccagata tagtcatgag actattctaa ccgctaagca cagaaaagca ctcccctttt
     2881 gtgcggtcag ctttgcgtgg cgttcgtttt ccgtcacaat aagacttttg ccaggacatt
     2941 gttatgcagg ccgaaatcct tctcaccctt aagctccaac aaaaattatt cgccgacccg
     3001 cgccggattt cgctactaaa acacattgcg ctttccggtt ccattagcca gggagcgaaa
     3061 gatgccggta tcagctataa aagcgcctgg gatgccatta acgagatgaa tcagttaagc
     3121 gagcatattc tggttgagcg cgcaacaggc ggtaaaggtg gcggcggcgc agtactgacc
     3181 cgctatggtc agcgactgat tcagctctat gacttactgg cgcaaatcca gcaaaaagcc
     3241 tttgatgtgt taagtgacga tgacgccctg ccgctgaaca gcctgctggc cgcgatctca
     3301 cgtttttcac tgcaaaccag cgcccgtaac cagtggttcg gtaccatcac cgcccgcgat
     3361 catgatgaca ttcaacagca tgttgatgtc ttactggctg acggaaaaac acgcctgaaa
     3421 gtcgcaatta ccgcacaaag cggcgcacgt ctggggctgg atgaaggcaa agaagtgttg
     3481 atattgctaa aagcgccgtg ggtaggcatt actcaggacg aagcggtcgc acaaaacgcc
     3541 gacaaccaat taccgggtat tattagtcat attgagcgcg gcgcagagca gtgcgaagta
     3601 ttaatggcgc tacccgacgg gcaaacactg tgcgccacag tgccggtaaa tgaagcgact
     3661 tctcttcagc aagggcagaa tgtcacggcc tactttaatg ccgacagcgt gattatcgcc
     3721 acgctgtgct aagcgtgttg acaatttgtt atgaaacacg tatccctgtc agtaatcgct
     3781 gcacaaaatg ggatataaaa tgtcatcgtt gcaaattttg caaggcacgt ttcgtcttag
     3841 cgacacaaaa acgctgcaat tgcctcagct aacgttaaac gcgggtgata gttgggcgtt
     3901 tgtcggttcg aatggaagcg ggaaatcggc cctggcccgc gcgctggcgg gggaacttcc
     3961 gcttttgaaa ggtgaacggc aaagccagtt ttcccacatc actcgtctct ccttcgagca
     4021 attgcaaaaa ctcgtcagcg acgaatggca gcggaataac accgatatgc tcggccctgg
     4081 cgaagatgac accggacgca ctacggctga aatcattcag gatgaagtaa aggatgcacc
     4141 gcgttgcatg caactggcgc agcagttcgg tattaccgcc ctcctcgacc gacgctttaa
     4201 atacctttcc actggcgaga cgcgaaaaac cctgctgtgt caggcgctga tgtcggagcc
     4261 tgacttgttg attcttgatg agtcgttcga tggcctggat gttgcctcac gtcagcagct
     4321 ggctgagcga ctcgcctcgt tacatcagtc cggtattact ctggtactgg tgctcaatcg
     4381 cttcgatgag atcccggagt ttgtccagtt tgctggcgtg ctggcggatt gcacgttagc
     4441 ggaaactggc gctaaagagg aactgctcca acaagcactc gtcgcgcaac tggcgcatag
     4501 tgaacagctt gaaggtgtgc aactgccgga gccggatgaa ccttcagcac gtcacgcctt
     4561 acccgccaac gaaccgcgca ttgtgctgaa caatggcgtg gtttcttata acgatcgccc
     4621 cattcttaat aaccttagct ggcaggtgaa tccaggtgaa cactggcaaa ttgtcgggcc
     4681 aaatggtgca ggaaaatcga cgttattaag cctggttact ggcgatcatc cgcaaggtta
     4741 cagcaacgat ttgacgcttt tcggacgacg tcgcggcagc ggcgaaacca tctgggatat
     4801 caaaaagcat atcggttacg tcagcagtag tttgcatctg gattaccggg tcagcactac
     4861 cgtgcgtaat gtgattcttt ctggctattt tgattcgatt ggcatttatc aggccgtttc
     4921 ggatcgccag caaaaactgg tgcagcagtg gctggatatt ctcggcattg ataaacgtac
     4981 ggctgacgct ccgttccata gtctttcctg gggacagcag cgtctggcgc tgatcgtccg
     5041 cgcactggtg aaacatccga cgttgcttat tctcgatgaa ccactacagg ggcttgatcc
     5101 gctcaatcgc cagcttatcc gccgttttgt tgatgtgctg attagcgaag gtgaaacgca
     5161 attgttattt gtttcgcacc acgctgaaga tgcgcctgcc tgtattaccc atcgccttga
     5221 gttcgtgccg gacggtggac tctatcgcta tgtgctgaca aaaatatatt gagtcggtag
     5281 tgctgacctt gccggaggcg gcctaagcgc cctctccggc caacggttcg acgcatgcag
     5341 gcatgaaacc gcttcttttt tcagataaaa agcgcaatca ttcataaacc ctctgtttta
     5401 taatcactta atcgcgcata aaaaacggct aaattcttgt gtaaacgatt ccactaattt
     5461 attccatgtc acacttttcg catctttgtt atgctatggt tatttcatac cataagccta
     5521 atggagcgaa ttatgagagt tctggttacc ggtggtagcg gttacattgg aagtcatacc
     5581 tgcgtgcaat tactgcaaaa cggtcatgat gtcatcattc ttgataacct ctgtaacagt
     5641 aagcgcagcg tactgcctgt tatcgagcgc ttaggcggca aacatccaac gtttgttgaa
     5701 ggcgatattc gtaacgaagc gttgatgacc gagatactgc acgatcacgc tatcgacacc
     5761 gtgatccact tcgccgggct gaaagccgtc ggcgaatcgg tacaaaaacc gctggaatat
     5821 tacgacaaca atgtcaacgg cactctgcgc ctgattagcg ccatgcgcgc cgctaacgtc
     5881 aaaaacttta tttttagctc ctccgccacc gtttatggcg atcagcccaa aattccatac
     5941 gttgaaagct tcccgaccgg cacaccgcaa agcccttatg gcaaaagcaa attgatggtg
     6001 gaacagatcc tcaccgacct gcaaaaagcc cagccggact ggagcattgc cctgctgcgc
     6061 tacttcaacc cggttggcgc gcatccgtcg ggcgatatgg gcgaagatcc gcaaggcatt
     6121 ccgaataacc tgatgccata catcgcccag gttgctgtag gccgtcgcga ctcgctggcg
     6181 atttttggta acgattatcc gaccgaagat ggtactggcg tacgcgatta catccacgta
     6241 atggatctgg cggacggtca cgtcgtggcg atggaaaaac tggcgaacaa gccaggcgta
     6301 cacatctaca acctcggtgc tggcgtaggc agcagcgtgc tggacgtggt taatgccttc
     6361 agcaaagcct gcggcaaacc ggttaactat cattttgcac cgcgtcgcga aggcgacctt
     6421 ccggcctact gggcggacgc cagcaaagcc gaccgtgaac tgaactggcg cgtaacgcgc
     6481 acactcgatg aaatggcgca ggacacctgg cactggcagt cacgccatcc acagggatat
     6541 cccgattaag gaacgaccat gacgcaattt aatcccgttg atcatccaca tcgccgctac
     6601 aacccgctca ccgggcaatg gattctggtt tcaccgcacc gcgctaagcg cccctggcag
     6661 ggggcgcagg aaacgccagc caaacaggtg ttacctgcgc acgatccaga ttgcttcctc
     6721 tgcgcaggta atgtgcgggt gacaggcgat aaaaaccccg attacaccgg gacttacgtt
     6781 ttcactaatg actttgcggc cttgatgtct gacacgccag atgcgccaga aagcaacgat
     6841 cctttgatgc gttgccagag cgcgcgcggt accagccggg tgatctgctt ttcaccggat
     6901 cacagtaaaa cgttgccaga actgagcgtt gcggcattga cggaaatcgt caaaacctgg
     6961 caggagcaaa ccgcagagct gggaaaaacg tacccgtggg tgcaggtctt tgaaaacaaa
     7021 ggtgcggcga tgggctgctc taacccgcat ccgcacggac agatttgggc aaatagcttc
     7081 ctgcctaacg aagctgagcg cgaagaccgc ctgcaaaaag aatattttgc cgaacagaaa
     7141 tcaccaatgc tggtggatta tgttcagcgc gagctggcag acggtagccg taccgttgtc
     7201 gaaaccgaac actggttagc cgttgtgcct tactgggctg cctggccgtt cgaaacgcta
     7261 ctgctgccca aagcccacgt tttgcggatc accgatttga ccgacgccca gcgcagcgat
     7321 ttggcgctgg cgttgaaaaa gctgaccagt cgttatgaca acctcttcca gtgctccttc
     7381 ccctactcta tgggctggca cggcgcacca tttaatggcg aagagaatca acactggcag
     7441 ctgcacgcgc acttttatcc gcctctgctg cgctccgcca ccgtacgtaa atttatggtt
     7501 ggttatgaaa tgctggcaga gacccagcga gacctgaccg cagaacaggc agcagagcgt
     7561 ttgcgcgcag tcagcgatat ccattttcgc gaatccggag tgtaagaaat gagtctgaaa
     7621 gaaaaaacac aatctctgtt tgccaacgca tttggctacc ctgccactca caccattcag
     7681 gcgcctggcc gcgtgaattt gattggtgaa cacaccgact acaacgacgg tttcgttctg
     7741 ccctgcgcga ttgattatca aaccgtgatc agctgtgcac cacgcgatga ccgtaaagtt
     7801 cgcgtgatgg cagccgatta tgaaaatcag ctcgacgagt tttccctcaa tgcgcccatt
     7861 gtcgcgcatg aaaactatca atgggcgaac tacgttcgtg gcgtggtgaa acatctgcaa
     7921 ctgcgtaaca acagcttcgg cggtgtggac atggtgatca gcggcaatgt gccgcagggt
     7981 gccgggttaa gttcttccgc ttcactggaa gtcgcggtcg gaaccgtatt gcagcagctt
     8041 tatcatctgc cgctggacgg cgcacaaatc gcgcttaacg gtcaggaagc agaaaaccag
     8101 tttgttggct gtaactgcgg gatcatggat cagctaattt ccgcactcgg caagaaagat
     8161 catgccttgc tgattgactg ccgctcactg gggactaaag cagtttccat gccgaaaggt
     8221 gtggctgtcg tcatcatcaa cagtaacttc aaacgtaccc tggttggcag cgaatacaac
     8281 acccgtcgtg aacagtgcga aaccggtgcg cgtttcttcc agcagccagc tctgcgcgat
     8341 gtcaccattg aagagttcaa cgctgttgca catgagctgg acccaatcgt ggcgaaacgc
     8401 gtgcgtcata tcctgactga aaacgcccgc accgttgaag ctgccagcgc gctggagcaa
     8461 ggcgatctga aacgtatggg cgagttgatg gcggagtctc atgcctctat gcgcgatgat
     8521 ttcgaaatca ccgtgccgca aattgacact ctggtagaaa tcgtcaaagc agtgattggc
     8581 gacaaaggtg gcgtacgcat gaccggcggc ggatttggcg gctgtatcgt cgcgctgatc
     8641 ccggaagagc tggtgcctgc cgtacagcaa gctgtcgctg aacaatatga agcaaaaaca
     8701 ggtattaaag agacttttta cgtttgtaaa ccatcacaag gagcaggaca gtgctgaacg
     8761 aaactcccgc actggcaccc gatggtcagc cgtaccgact gttaactttg cgtaacaacg
     8821 cagggatggt agtcacgctg atggactggg gtgcgacttt actttcagct cgtattccgc
     8881 tttccgatgg cagcgtccgc gaggcgctgc tcggctgtgc cagcccggaa tgctatcagg
     8941 atcaggccgc gtttctgggg gcctctattg gtcgttatgc caaccgcatc gccaatagcc
     9001 gttatacctt tgacggtgaa acggtgacgc tttcgccaag tcagggcgtt aaccagctgc
     9061 acggcggccc ggaagggttc gacaaacgtc gctggcagat tgtgaaccag aacgatcgtc
     9121 aggtgctgtt tgccctgagt tcagatgatg gcgatcaggg cttcccgggt aatctcggcg
     9181 cgacggtgca atatcgtctg accgacgata accgtatctc cattacttat cgcgccacag
     9241 ttgataaatc ttgcccggtg aatatgacta atcacgtcta tttcaatctt gacggcgagc
     9301 agtctgacgt gcgcaatcac aagttgcaga ttctggcggg cgaatatctg ccggttgatg
     9361 aaggcggcat tccgcacgac ggcctgaaat ctgtcgccgg aacctctttt gatttccgca
     9421 gcgccaaaat catcgccagt gagtttcttg ccgacgacga tcagcgcaaa gtgaaaggtt
     9481 acgatcacgc attcttgtta caggccaaag gcgatggcaa gaaagtggcg gcgcatgtct
     9541 ggtcagcaga tgaaaaattg cagctgaagg tctacaccac cgctcccgct cttcaattct
     9601 actccggcaa cttcctcggc ggcacaccgt cgcggggaac cgaaccttac gccgactggc
     9661 aaggactggc gttggaaagc gagtttctgc cggacagccc gaaccaccct gaatggccgc
     9721 aaccggactg cttcctgcgt cctggcgaag agtattccag cctgacggaa tatcagttta
     9781 ttgctgagta atgttaagcc ctcgtcatga gggctttatc ttcatattgt tcaaatcacc
     9841 agcaaacacc gacatatttg caactcaata ttcacaacaa ccttacactg cgccactatt
     9901 ttcgctatgg ttatgcgtaa gcattgctgt tgcttcgtca cggcaatata atgagaatta
     9961 ttatcataaa aagataattt gaggagtaag tatatggctg taactaagct ggttctggtt
    10021 cgtcatggcg aaagtcagtg gaacaaagaa aaccgtttca ccggttggta cgacgtggat
    10081 ctgtctgaga aaggcgtaag cgaagcaaaa gcagcaggta agctgctgaa agaggaaggt
    10141 tacagctttg actttgctta cacttctgtg ctgaaacgcg ctatccatac cctgtggaat
    10201 gtgctggacg aactggatca ggcatggctg cccgttgaga aatcctggaa actgaacgaa
    10261 cgtcactacg gtgcgttgca gggtctgaac aaagcggaaa ccgccgaaaa gtatggcgac
    10321 gagcaggtga aacaatggcg tcgtggtttt gccgtgactc cgccggaact gactaaagat
    10381 gatgagcgtt atccgggtca cgatccgcgt tacgcgaaac tgagcgagaa agagctgccg
    10441 ctgactgaaa gcctggcgct gaccattgac cgcgtgatcc cttactggaa tgaaactatt
    10501 ctaccgcgta tgaagagcgg tgagcgcgtg atcatcgctg cacacggtaa ctctttacgt
    10561 gcgctggtga aatatcttga taacatgagt gaagaagaga ttcttgagct gaatatcccg
    10621 accggcgtgc cgctggtgta tgagttcgac gagaatttca aaccgctgaa acgctattat
    10681 ctgggtaatg ctgacgagat cgcagcgaaa gcagcggcgg ttgctaacca gggtaaagcg
    10741 aagtaaacgt cattcgttta aaaatgagaa agccgactgc aagtgagtcg gcttttttgt
    10801 tgctaacaat ggagcacatt gcctgatgcg acgctgcgcg tcttatcagg cctgtggtga
    10861 ttcatcggat acgccactct gacggcgcat ccgacaatta aaccttaccc gcgacgcgct
    10921 tttactgcat tcgccagttg acgtaacaga gcatcggtat cttcccagcc aatgcaggca
    10981 tcggtgatgc tcttaccgta ggccagcggc tccccgctat cgaggctctg attgccttcc
    11041 accagatggc tttccaccat cacgccaata atggcctttt cgccaccggc aatctgctgg
    11101 caaacgtcag cacaaacatc catctgcttt ttgaattgtt tggacgagtt agcatggctg
    11161 aaatcgatca tcacctgtgc tggcaggcct gctttgttca gcccttcttt cacttcagca
    11221 acgtgcttcg cgctgtagtt aggctcttta ccgccgcgca gaatgatatg gcaatcaccg
    11281 ttaccgctgg tattcacaat cgccgaatgc ccccatttag ttacggacag gaagcagtgc
    11341 ggcgcaccgg cggcattaat ggcatcgata gccactttaa tcgtaccgtc tgtgccattt
    11401 ttaaaaccta ccggacaaga aagaccagac gccagttcgc ggtgcacctg cgattccgtg
    11461 gtacgtgcac caattgcgcc ccagctcatc aggtcagcga gatactgagg agtgatcata
    11521 tcgagaaact cacccgccgc tggcagaccg ctgtcgttaa tatcaagcag caatttacgg
    11581 gctatacgca gaccgtcgtt gatctggaag ctgttatcca tatgcggatc gttaatcaac
    11641 cctttccagc ccaccgtggt acgcggcttt tcaaaataga cgcgcattac gatttccagc
    11701 tcatctttca gctcttcacg cagcgccagc aagcgagtgg catactcttt agccgcgaca
    11761 ggatcatgaa ttgagcacgg gccaatcacc accaacaggc gatcatcatt acctttcagg
    11821 atcttatgga tcgcttttcg ggcatgggca accgtattcg cggcattttc agttacgggg
    11881 aatttttcca gcaatgcgac aggaggaagt aactctttga tttctttgat gcgtaaatcg
    11941 tcgttctgat aattcatgtc tgttccagtg ttgccatact tatcttagtg aatgcaatac
    12001 ttccaatcta tatcttccgt cagaatgtgt aaacggggtt ttacactatg aacggattaa
    12061 tcctggaatt agcaagaaaa acgccagatt gtcgcgaaaa acgagatctc tcctacaatt
    12121 tctaactgta actcctttgt ttgttaatta tttcaagatt ctctgctgcg tttcataaac
    12181 tggctgaaat cttaaaccaa tgccttatat tcacctgcaa atgcactgtt ggaagaggtt
    12241 atccgacata acgaccataa caggagcatc ctatgaaaat gacaaaactg gccacattat
    12301 ttctgactgc cactctaagc cttgccagcg gtgccgcact ggctgccgat agcggagcgc
    12361 aaactaataa cggccaggca aacgccgcag ctgatgcggg ccaggtagcc cctgacgccc
    12421 gtgaaaatgt cgcgccaaat aacgtcgaca ataacggggt aaataccggt tctggcggca
    12481 caatgctgca ttcggatggt tcttcaatga acaatgacgg aatgaccaaa gatgaaaagc
    12541 acaagaacac catgtgcaaa gatggtcgat gtccggatat caataaaaag gtacaaatcg
    12601 gtgatggcat caataatgat gtcgatacca aaaccgacgg taccacgcag taaccctgca
    12661 aaatagccaa taatgggaga gcggagtctc tcccttttaa tttttcactt ctaaaagcag
    12721 tatgatgtca taaagttaag tgagaataat taagggatga ctcgtcatgg cgcactcaca
    12781 ctcacacacg tcctcacacc tgccagaaga taataatgct cgtcgcttgt tgtatgcttt
    12841 cggcgtgact gccgggttta tgctggtaga agtcgttggt ggttttcttt ctggttctct
    12901 ggcattgctg gccgatgcgg gtcatatgtt gaccgatact gccgccctgc ttttcgccct
    12961 actcgccgtg caattttcac gtcgtcctcc caccattcgc cacactttcg gttggctaag
    13021 actgaccacg ctcgcggcgt ttgtgaatgc catcgccctc gtggtaatta ccattttgat
    13081 tgtctgggag gcgatagaac gcttccgcac gccgcgtccg gtcgagggcg gcatgatgat
    13141 ggcaattgcc gtggctgggt tgctggcaaa tatactttct ttctggttac ttcatcacgg
    13201 cagcgaagag aaaaacctca acgtacgagc agcggcattg catgtgctcg gggacctgtt
    13261 gggttcggtt ggggctatta ttgccgcgtt gattattatc tggaccggct ggacacctgc
    13321 tgaccccatt ctctcgatag tggtgtcgct tctggttctg cgtagcgcgt ggcgactgtt
    13381 gaaagatagc gtgaatgaat tacttgaagg tgcaccggta tcgctggata tcgctgaact
    13441 gaagcgtcgt atgtgccggg aaatcccgga agtccgcaat gtgcaccatg tacatgtatg
    13501 gatggtaggc gagaagccgg tgataacgct gcatgtgcag gtgatcccgc cacacgatca
    13561 cgatgccttg ttggatcaga tccaacacta cctgatggat cactatcaga ttgagcacgc
    13621 caccattcag atggaatatc aaccttgtca tgggccagac tgccacctga atgaaggggt
    13681 gtctggccat tcacatcatc accattaatg ggacagcgcg cgtgagcctc tttcacgtgc
    13741 gctgttgatc cacatccgac tgccgttgag cgcaataaag gtcaggatga tgtactccag
    13801 agacatggcg taaacgccct gaagtgcaaa aataacgacg ctaatcacgt taataatcac
    13861 ccacaacagc cagttttcca catacttacg cgtcatcaga atcattgcca cgatagataa
    13921 caccatcatg catgaatccc agaacgggaa agcgtccggt tgcagttcag gcatcacaac
    13981 ctgtaatcct aatgcttgca tgatcatgac tgccacgcgg gtcaaaaatg caaacaccgg
    14041 attgataaat accgtcatca gaccaatcga aacaacgcaa accgccaacc agctgagcgc
    14101 cttcggcaat ggtaaccagc gaattttcaa ctccgcctcg ttctgactgg tttgtcgcga
    14161 ccacgcatac caaccgtaaa tattcgcggc aaagaaaaac acctgtaata gcaggctggc
    14221 atacagctga atctgaaaga aaataatgcc aaacaaggtg acgttgatca ggccaaagaa
    14281 gtagttgctg atcttctcca gactggcaag gccaatacac agcaacccgg cgatcgtgcc
    14341 taccgcttcg atccatgaga gatcataacc gcctgcccct attggtatat gtaccaggat
    14401 attctgcaca ctaaaaaaat ccatctttcc cccaaagcgt aacgttacgc cttattattc
    14461 gttatccacg tagtgtagcc gcaaaatcca gcatacgatt gagcggcacc agcgccctct
    14521 ctcgcagcct ttcatcaaga acctcgtgat tgcttccttc ctgttctaat gcctctgcga
    14581 tggactgaag gccattcatg gccatccacg gacaatgcgc gcag
//
LOCUS       DAJTYZ010000100        14394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000100 DAJTYZ010000000
VERSION     DAJTYZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.100"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO596_002949"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO596_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6718752.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO596_002950"
     CDS             1828..3147
                     /locus_tag="OO596_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6718753.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3519..4901
                     /gene="dosC"
                     /locus_tag="OO596_002951"
     CDS             3519..4901
                     /gene="dosC"
                     /locus_tag="OO596_002951"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6718754.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4926..7325
                     /gene="dosP"
                     /locus_tag="OO596_002952"
                     /gene_synonym="pdeO"
     CDS             4926..7325
                     /gene="dosP"
                     /locus_tag="OO596_002952"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR6718755.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7584..8165
                     /gene="ddpX"
                     /locus_tag="OO596_002953"
     CDS             7584..8165
                     /gene="ddpX"
                     /locus_tag="OO596_002953"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR6718756.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8179..9729
                     /locus_tag="OO596_002954"
     CDS             8179..9729
                     /locus_tag="OO596_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6718757.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9731..10753
                     /locus_tag="OO596_002955"
     CDS             9731..10753
                     /locus_tag="OO596_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6718758.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10750..11646
                     /locus_tag="OO596_002956"
     CDS             10750..11646
                     /locus_tag="OO596_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6718759.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11643..12629
                     /locus_tag="OO596_002957"
     CDS             11643..12629
                     /locus_tag="OO596_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6718760.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12658..13548
                     /locus_tag="OO596_002958"
     CDS             12658..13548
                     /locus_tag="OO596_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6718761.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OO596_002959"
     CDS             complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OO596_002959"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6718762.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3572 a         3555 c         3627 g         3640 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gcttttttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggca cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcaaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcatc cgcaacccac gtcaatgaaa
      841 tgagcaaccc agggcgcgac tatccgttgg ctatgttact gctgatggta gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcacagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcta ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ctctaccgat atgtatgttg
     1501 aattactggt cgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgctcgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcatatatg gatatctgct cccgaaacga gaaattagcg
     1861 attagaagac cagcgatact ggttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggtgt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggtataaaca cggtcttttt ccaggttaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccat ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgca aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaagt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ctaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcacacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtca atctcgcacg ttgcgttttg
     3481 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     3541 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc actcattcgt gctaaagccg
     3601 cggaaattgc cgttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     3661 tcgacccgca tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     3721 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     3781 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     3841 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     3901 attccgccgc agaaaaactt caggtctatc atttctcgat taacagtatt gatatcgcga
     3961 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     4021 attatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     4081 caatactttc atgggaaata gatattatct ataaaatcct gctggattct gatttaggca
     4141 gtagtttgcc attaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     4201 attttagtgg cattgccgaa gtaggccata tctcccgtct cattcaggat ttcgacggta
     4261 ttttcaatca aaccatgcgt aacaccagga atttgaataa cagaagtctg cgggtgaaat
     4321 ttttattaca gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg
     4381 aagaagtatc gcgccacgaa gtcggtatgg atgtactaac gaaattactt aaccgccgtt
     4441 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     4501 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     4561 ctggcgatga aattctgcgt aaagtctctc aggcctttta tgacaacgtc cacagtagtg
     4621 attatgtttt tcgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa
     4681 acgaaacgtt acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag
     4741 ccgcaaacgg cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc
     4801 atcctgacta tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac
     4861 gaggtagaaa ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg
     4921 taatcatgaa gctaaccgat gcggaaaatg ccgccgatgg catttttttc cccgcccttg
     4981 aacaaaatat gatgggcgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc
     5041 ccgccgcaga gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata
     5101 tgctgattcc gcgggatttg cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag
     5161 gtggtaaagc gcgcgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca
     5221 gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc cgaggggaaa gtttattacc
     5281 tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa agaacagacc cgacaattga
     5341 ttattgccgt tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg
     5401 tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta
     5461 tgcagcccga tacactcctg aacattcctg aattccctgc cgataaccgc attcgtttac
     5521 aacagttgct atggaaaacc gcccgcgatc aggacgaatt tctgctgttg acacgcaccg
     5581 gtgaaaaaat ctggattaaa gcctctatca gcccggttta tgacgtgctc gcgcatctgc
     5641 agaacctggt aatgaccttc tcggatatca ccgaagaacg gcagatccgc cagcttgaag
     5701 gcaatattct cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaatcattt
     5761 gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg
     5821 ggatgccgat acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct
     5881 ggtcagcgac cattcgtcag cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct
     5941 cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc agatatcagc cagcatatgg
     6001 ccgcgctggc gctggaacag gaaaaaagcc gtcagcatat tgaacaactc atccaatttg
     6061 atccgatgac cggtctgcca aatcgcaata acctgcacaa ttacctcgat gaccttgtcg
     6121 acaaagccgt ctctccggtg gtgtatctca tcggtgttga ccatattcag gatgtgattg
     6181 atagccttgg ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg
     6241 aaaaactcaa accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga
     6301 gcctggaaaa cgacgtcagt aacattaccc aaatcgccga tgagctacgg aatgtggtca
     6361 gcaagccgat aatgattgac gataaaccat tcccgctaac cttgagtatt ggcatcagct
     6421 acgacgtggg taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata
     6481 ttcgcaagaa tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa
     6541 aagagcgttt ggttttaggc gcagcgctga aagaagcgat tagcaataac caactgaaac
     6601 tggtttacca gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg
     6661 ctcgctggca cgatccccag catggtcatg tacccccttc acggtttatt cctctcgcag
     6721 aagagattgg tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt
     6781 tagcagaatg gcgtagccag aatattcata tcccggcgtt atccgtgaac ttgtcggcgc
     6841 tgcactttcg cagtaatcag ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta
     6901 ttgacggcca ccagctgacg gtagaaatca cggaaagcat gatgatggaa cacgataccg
     6961 aaatctttaa gcgcattcag atcctgcgcg atatgggcgt gggcttatcg gtagatgatt
     7021 ttggcacggg cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa
     7081 ttgacaaaag ttttgtcgat cgttgtctga cggaaaaacg catccttgcc ttacttgaag
     7141 ccattaccag cattgggcaa agcctcaatt taaccgtcgt ggcggaaggc gtcgaaacca
     7201 aagagcaatt tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt
     7261 cccgccccct acccgccgag gaaattccag gctggatgag cagcgtgtta ccgctgaaaa
     7321 tctgacaaat tcctctcgcc cgcactcgcg ggttttcatt taacgtgacg ctgtcactta
     7381 aatactgtga tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc
     7441 aaaactgcac tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag
     7501 cattctggcg cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt
     7561 ttgtcttaat acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga
     7621 tcttccctga tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag
     7681 ctatttacca gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa
     7741 gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac
     7801 aacaagcaca ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga
     7861 cggtcggttc taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg
     7921 ggaacatcct ggatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc
     7981 acccttccgt cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg
     8041 gtggcggttt tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga
     8101 gttaccctct gctcgctgat caattctctt gttttatatc ccctggcaca cagcacgtca
     8161 gttaatcagg aaactgtcat gaagagatcg atatcgtttc gtcccacatt gctcgcgctc
     8221 gtccttgcca caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt
     8281 ggtaaggccg ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg
     8341 acagtgacct acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc
     8401 tcaaccgacg ttgaaggcga tctggcaagt agctggaaag cgtctgacga tcaaaaagag
     8461 tggacgttca ccctgaagga caatgctaaa tttgccgatg gcacacctgt cactgccgaa
     8521 gcagtaaaac tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt
     8581 cccaaagatt taaagattga tactcccgac gaacatacgg tgaagtttac ccttagccaa
     8641 ccattcgcac cgttcctcta cacgctagcg aatgacggtg catccattat caatccggcg
     8701 gtcttaaagg aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt
     8761 tccggaccat ttatgctgaa aagcgggcaa aaaggtcagc aattagttct ggtgccaaat
     8821 ccgcattacc ccggcaataa accgaacttt aagcgagtat cggtaaaaat tatcggtgaa
     8881 agtgcctccc gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg
     8941 gtggatcaac tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca
     9001 ctgcgcgtta cctatctgta tctgaataac agcaaagcgc ctcttaatca ggcggatctg
     9061 cgtcgggcca tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt
     9121 aacggaaaac agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca
     9181 atgcaataca accatgacga aacgaaagcc aaagccgaat gggataaagt gacgagcaaa
     9241 cccaccagcc tgacgtttct ctactccgat aacgatccga actgggagcc tattgctctg
     9301 gcgacacaat ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac
     9361 gccaccatgc gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt
     9421 ccggattttg ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt
     9481 ctgccgggta accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg
     9541 cttgcgacca ccgaccagac gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc
     9601 attgatgacg ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa
     9661 gaggtgaaag gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg
     9721 agtaaataac gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt
     9781 ggtggcgggc gtctgcgtga ttacgtttat tatctcgcat ctgatccctg gcgatccggc
     9841 gcggttactg gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact
     9901 gggactggac cagccactgt atgtacagtt ttaccgctac ttcagcgacc tgtttcatgg
     9961 tgacctggga acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt
    10021 cccggcgacg ctggaactgg ctttttgtgc cctgctgctg gcactcctga ttggcatccc
    10081 gctgggcata ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat
    10141 ggccattacc ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt
    10201 ttatggtcat ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc
    10261 aacgcacgtt accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt
    10321 cttcaatgcg ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg
    10381 aattgtcgct cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg
    10441 taccgcccgg gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa
    10501 tgcgttgatc ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc
    10561 agtgctcacc gaaaccgttt ttgcctggcc cggaatgggt gcatgggtag taacatcaat
    10621 acaggcgctc gacttcccgg cagtgatggg ctttgccgtc gtggtttcat ttgcttatgt
    10681 gctggtcaac ctggtggtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg
    10741 aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga
    10801 tttaacggtg caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca
    10861 gtcatcattg tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat
    10921 cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc
    10981 accgatgaag tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt
    11041 ctcgccggat tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta
    11101 tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg
    11161 tcgattcctt cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt
    11221 aacgccatgc tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg
    11281 caagcattag ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg
    11341 cgttggcatc tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag
    11401 gcatcgctgg atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg
    11461 ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag ctaatggtcg caactatgta
    11521 ctcgatcagt ggtggtattg cgcatttccg ggggcagcga ttttgcttac cgccgtcggg
    11581 tttaatctct ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag
    11641 tcatgaccca acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg
    11701 gttatgttca cgtgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc
    11761 tggtgggaga atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac
    11821 cgacgggcag ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta
    11881 acgctcggga gaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg
    11941 aaccgatgac cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc
    12001 gccatcatca accaataagt cgtcgggaag ccagagctaa agcgattacc ctgctggaag
    12061 agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg
    12121 gtatgcgcca gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg
    12181 ccgacgaacc gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta
    12241 aacataaagc tcgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg
    12301 tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg
    12361 tgacggcaga cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg
    12421 caccagaaca tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc
    12481 tcacccattt gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt
    12541 gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt
    12601 atccgcagca ggaggtcata agtatctgac acgttattaa cgttacgcga cgtccatatc
    12661 aatttcccgg cccgtaaaag ctggcttggt aaaactacgg aacatgttca tgcgattaat
    12721 ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggtga gtcaggctgc
    12781 ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac
    12841 atccgttcag gatcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt
    12901 aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt
    12961 agtgaacaac agcggcgagc gttggcaaag gagttggctg tgcaggtggg tattcgtccg
    13021 gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt
    13081 gccagagcac tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg
    13141 gatatctccg tgcaggcgca gatcctcaat ttactggtaa cgctacagga aaatcacggg
    13201 ctgacttatg tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg
    13261 gcggtgatgt atctcgggca gattgtagaa ctgggcgacg cgcagcaggt gctgacggca
    13321 cctgcacatc catacacccg attattgctg gattccctcc ccgccattga taaaccgctg
    13381 gaggaagaat gggcattatg taaaacggat ctgccgggaa accgcacgtt gccacaaggc
    13441 tgttttttct acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta
    13501 gcaataaggg aggacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa
    13561 cgcatcaggc attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt
    13621 aatccagcgt aatttccgct ttcagtacct gagaaaccgg gcatcctgct tttgctttct
    13681 ggatgatgcc gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg
    13741 cgattttcgt aatcgcaaaa tcggcatcca ctttatccag cgacacatcg gcggtggtat
    13801 caatcgatgt tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac
    13861 atgcggcatg cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac
    13921 gcgtgttaaa tccatacggc tgttggttca gcacgccact ctgggtggat actgttccct
    13981 tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg
    14041 ctctcctgtg ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc
    14101 aggatattcc gaataaaata cggcgaaaat attacttctg cacttaaatt aaattcccac
    14161 aatagataac cgggaatata tatctcaaga gatattgcat caatatcaat caactcacca
    14221 caataataat acatatcaat atcatggagt taaaatataa aaatcatcaa ccaggactaa
    14281 tcttaacaac gaagcggcaa atatttgcca tgctgaatgt gctttataaa tctgcgatcc
    14341 gtagcagaca ccataaatac acagacacgg agaatcacta tgtttactta ttat
//
LOCUS       DAJTYZ010000101        14306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000101 DAJTYZ010000000
VERSION     DAJTYZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.101"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="OO596_002960"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="OO596_002960"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6718763.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="OO596_002961"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="OO596_002961"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6718764.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OO596_002962"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OO596_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR6718765.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="OO596_002963"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="OO596_002963"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6718766.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="OO596_002964"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="OO596_002964"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6718767.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OO596_002965"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OO596_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6718768.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OO596_002966"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OO596_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6718769.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OO596_002967"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OO596_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6718770.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="OO596_002968"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="OO596_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6718771.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="OO596_002969"
     CDS             complement(13987..>14306)
                     /locus_tag="OO596_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6718772.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAJTYZ010000102        14248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000102 DAJTYZ010000000
VERSION     DAJTYZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14248
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.102"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1455
                     /locus_tag="OO596_002970"
                     /pseudo
     CDS             1..1455
                     /locus_tag="OO596_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1543..2514)
                     /gene="lpxM"
                     /locus_tag="OO596_002971"
                     /gene_synonym="msbB"
     CDS             complement(1543..2514)
                     /gene="lpxM"
                     /locus_tag="OO596_002971"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6718773.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2634..3956)
                     /gene="mepM"
                     /locus_tag="OO596_002972"
     CDS             complement(2634..3956)
                     /gene="mepM"
                     /locus_tag="OO596_002972"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6718774.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3972..4904)
                     /gene="znuA"
                     /locus_tag="OO596_002973"
     CDS             complement(3972..4904)
                     /gene="znuA"
                     /locus_tag="OO596_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6718775.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4983..5738
                     /gene="znuC"
                     /locus_tag="OO596_002974"
     CDS             4983..5738
                     /gene="znuC"
                     /locus_tag="OO596_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6718776.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5735..6520
                     /gene="znuB"
                     /locus_tag="OO596_002975"
     CDS             5735..6520
                     /gene="znuB"
                     /locus_tag="OO596_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6718777.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6667..7677)
                     /gene="ruvB"
                     /locus_tag="OO596_002976"
     CDS             complement(6667..7677)
                     /gene="ruvB"
                     /locus_tag="OO596_002976"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6718778.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7686..8297)
                     /gene="ruvA"
                     /locus_tag="OO596_002977"
     CDS             complement(7686..8297)
                     /gene="ruvA"
                     /locus_tag="OO596_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6718779.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8436..8501)
                     /gene="yobI"
                     /locus_tag="OO596_002978"
     CDS             complement(8436..8501)
                     /gene="yobI"
                     /locus_tag="OO596_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6718780.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8574..9176
                     /locus_tag="OO596_002979"
     CDS             8574..9176
                     /locus_tag="OO596_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6718781.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9178..9699)
                     /gene="ruvC"
                     /locus_tag="OO596_002980"
     CDS             complement(9178..9699)
                     /gene="ruvC"
                     /locus_tag="OO596_002980"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6718782.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9734..10474)
                     /locus_tag="OO596_002981"
     CDS             complement(9734..10474)
                     /locus_tag="OO596_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6718783.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10503..10955)
                     /gene="nudB"
                     /locus_tag="OO596_002982"
     CDS             complement(10503..10955)
                     /gene="nudB"
                     /locus_tag="OO596_002982"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6718784.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11073..12845)
                     /gene="aspS"
                     /locus_tag="OO596_002983"
     CDS             complement(11073..12845)
                     /gene="aspS"
                     /locus_tag="OO596_002983"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6718785.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13155..13721
                     /locus_tag="OO596_002984"
     CDS             13155..13721
                     /locus_tag="OO596_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6718786.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14076..>14248
                     /locus_tag="OO596_002985"
     CDS             14076..>14248
                     /locus_tag="OO596_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6718787.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTAD"
BASE COUNT         3507 a         3629 c         3370 g         3742 t
ORIGIN      
        1 gaatttttta taactattac taataatatg acatcaattc ttcgcagtga gtttgatgaa
       61 atatggaaag cgatgctgac cttgcaattc catgacatcc ttcccggctc atgtatttca
      121 agagtttatc atgaaactga aaaggaatat cttaaactag aagcaaaaac cgagaaaatt
      181 atttctgatg cgcagtctac tttattaagt aaaattgaca cttcttctta taaagatccg
      241 cacattttgt ttaataccac ctgttttgct cgtaacgaat ggatcaatat aaacaacaac
      301 tggctcaaag cacgagtaaa ctcttatggt tatgctgtga ttgttccaaa gaataagatc
      361 gtaaatggtc tgaaggcaga atctcaaagt attgaaaata attacattaa acttcttttt
      421 tcagaaagcg gtgatttaat ttcgctatat gataaaagat atggtaaaga atacattact
      481 gaaaatatgc acagtgagat ccgtgcatac cacgaagatg caggtttttt cgcggcctgg
      541 gattttgctt caaattatcg tgatggggaa tcatatgttt tactcgctga aaaaatgaca
      601 actgtgatca gcgggcctaa aactacgatg acgctgattt atcactacaa ttcctcttat
      661 cttcgtttag cctttaccct gacacaggat agtccacgcg ttgatgtcca aacgtttatt
      721 gattggcacg aacctaatgt ttctctcaaa gtaaaattcc cggtgtcagt acaaacatct
      781 cttgcacaat gtcaaattca gttcggtgta attgacagac caacgcatag cgatgattcg
      841 ttcgcttttg ccaaagatga aataccagca catcactggg tcgacctttc tgatcgagaa
      901 actggcatag ctttaatagc aaaaaacaaa tatggctatc gtgtcaaaga ccagacgctt
      961 gaattaactt tgctgcgttc gcagcataaa cctggggaag ttgttaagac agacaattat
     1021 gataatttcc tgattaataa ttttgcagat attggtaaac aaaaattcat attctcctta
     1081 tttcctcatc atggagatta ccgtgtgggt ggtgttatta atcaagccta tataatgaat
     1141 catcccttac aggtcgtccc tgtcaaaccg cacccgggtg aattaccccc ttcactatca
     1201 ttttttgcta tcgatactaa cagcgttatc atcgaaacaa taaaattagc tgaagacggc
     1261 gacggtataa tcgtaagatt atatgagagt catggattag aaatttcagc catgctttcg
     1321 tgagtatgta attatcacta cgttcaatat gtcgatcttc aagaaaataa gttagatgac
     1381 agtttttatt gcaatcaata ttgcaatctt gagttcaaac cttttgaaat tatcacgcta
     1441 cgattaactc aatagcgtac taacctaaaa actcaaaata gtgggaacac tcattgagat
     1501 tttttatcga gtacagcctc tcaataattt gagaggcttt ttttatttga taggataaag
     1561 atctttacgc ttatacggtt ggatttcgcc cggtttgcga gttttcagca attttaatat
     1621 ccaggtgtat tgttctggtc gcggaccaac aaaaatctcg acttcttcat tcatccgccg
     1681 cgcaatcgta tgatcatccg cctctaacag atcatccatc ggtgggcgca cctgaatcgt
     1741 cagacggtgc gtcttgccat cataaatcgg aaacagcgga acaacgcgcg cacggcacac
     1801 tttcatcaaa cgaccaatcg cgggcaacgt cgctttatag gtggcaaaga aatccacaaa
     1861 ttcgctgtgc tctgggccat gatcctgatc gggtaaataa tatccccagt acccctgacg
     1921 taccgactgg atgaatggtt taataccgtc atttctcgca tgcagacgac cgccaaagcg
     1981 acgacgcacc gtgttccaga cataatcaaa aaccgggttg ccctgattat ggaacatcgc
     2041 tgccattttc tgcccttgcg aggccatcag catggcagga atatcgacgg cccaaccgtg
     2101 cggcaccaga aagataactt tctcgttatt acgcctcatc tcttcgatga tctccagccc
     2161 ttgccagtca acgcgcggct gaattttctc cggtccgcgt attgccaact cagccatcat
     2221 tgccatcgct tgcggcgcgg tggcaaacat ctcatcaaca atcgcttcgc gttcagcttc
     2281 actacgttct ggaaagcaga gcgacagatt gattaacgca cgacggcgtg agctttttcc
     2341 cagtcgtccg gcaaaacgtc ccagccgtgc cagaatggga tcacggaact ttggcggcgt
     2401 taaagcgata cccgccatcg ctgctacacc cagccatgct ccccagtagc gcgggtggcg
     2461 aaaggattta tcaaactcag gaatgtattc gctattattt tttttcgttt ccatgctttt
     2521 ccagtttcgg ataaggcaaa aatcaatctg gtgatagtat agcggcgcaa cttgccccgc
     2581 accaaataaa aaaagccgat actgactgcg taccggctgc gaatggatgt taattaatca
     2641 aaccgtagct gcggcacaat ctctttggcc tgcgccagga attcgcgacg atcggagccg
     2701 gtcagccctt cggtacgcgg cagttttgcc gtcagcgggt ttacggcctg ctggtttatc
     2761 catacttcat agtgcagatg cggcccggtt gaacgtccgg tattaccgga aagcgcgata
     2821 cggtcgccac gtttcacctt ctgtcccggt ttcaccagaa tcttgcgcaa gtgcatataa
     2881 cgcgtggtgt agctgcgacc atgacgaata gccacataat aacctgctgc gccactgcgt
     2941 ttggcaacca ccacttcacc gtcacccact gaaagcactg gcgtaccctg cggcatggcg
     3001 aaatcaacac ctctgtgtgc tgcaacgcga ccggtcaccg gattagtacg acgcgggtta
     3061 aagttagacg agatacggaa ctgtttcgcc gtcgggaatc gcaagaatcc tttcgccaga
     3121 ccagtaccgt tacgatcgta gaatttgcca tcttcagcgc ggatagcgta ataatcttta
     3181 ccttctgaac gcaaacgtac gcccagcagc tggctttgct cacgtttacc atcaagcatt
     3241 tctcgagaca ttaacaccgc aaattcatcg ccttttttca gtttgcggaa atccatttgc
     3301 cactgcatgg ctttaatcac tgcgctcact tcggcgctgg ttaaaccggc gtttctggcg
     3361 ctggcaacaa agctcccccc gacggtacct ttcagcagat tgttaaccca ctctccttgc
     3421 tgcatttcgc tggtcatttt aaaaccgtta gcggcagtac ggtcataggt tcgggtttca
     3481 cgacgagaca cttcccaggt gaggcgctgc agttcgccgt ccgcggttaa tgtccaggag
     3541 agttgttgac cgattttcag gttacgcaat tctttgtcgg ccgcagccag ttgggtgata
     3601 tcacccatat caataccata ctgattgaga atgctgctta gcgtatcgcc agtggaaaca
     3661 acatattcat gcacgcccgc ttcaccggcg attttgtcat ccagttcgtc ctggggaatg
     3721 gcttcatctt cttgtgcagc ttgatcaatc ggctcactgg cttcaggtaa gagcgaacga
     3781 atttcgttct gttccagctc aatggttttg acaattggcg tggcatcgcg gtgataaaca
     3841 tagggccgcc agacagcgac ggccagagta agaacggtga gcgaccccaa cataacgcgg
     3901 tgtggtcgcg gtaaattatt aaacgccagg gcgacagagc gggctatctg ttgcacgtaa
     3961 tcacttcctc attaatctcc tttcaggcag ctcgcatact ggttggctaa ttgactcagg
     4021 aattctgaat agcttgtttt acccagtttg atattcgtcc ccaggggatc caacgttccc
     4081 atacgaacgg atgtccctcg tgcgacgctc tcaacgaccg ctggcctgaa ctgtggctca
     4141 gcaaaaacgc aggttgcttt ttgctcaacc aactgtgttc ttatttcatg taaacgctgc
     4201 gcgccaggtt gaatctcagg gttaacggta aaatgaccaa gcggtgtcag tccgaactgt
     4261 ttttcgaaat agccgtaagc atcgtgaaaa acgaaataac ctttcccctt gagcggcgcg
     4321 agctcgttac caacctgcgt ttcggttgag gctaattgtg cctcaaaatc cttcaggttg
     4381 gcgtcaagtt tggctcgact ttgcggcata agttccacta attttccatg gattgcaacc
     4441 gctgtagccc gcgctatctc tggggaaagc caaagatgca tgttgaaatc gccgtgatgg
     4501 tgatcttcgt cacttttttc cgcgtggtcg tgatcatcat catcgccgtg aatacttttc
     4561 atcagtagcg gtttcacatc ttcaagctgc gcaatcgtta cctgcttcgc tcctggtaat
     4621 ttgcttaccg gtttttgcat aaacgcttcc atctccgggc caacccaaac gactaagtcc
     4681 gcgttctgta agcgttttac atccgatggg cgcagtgaat aatcatgttc tgaagcgccg
     4741 tcaggaagta aaacctctgt ttctgttacc ccatcagcaa tggcagaagc gatgaaccca
     4801 acgggtttaa gcgaagcgac aacggcggca tctgcggcct gtgttgcacc tccccagaga
     4861 gcggcggata atgctgcgaa aagaagcgtt tttttatgta acataatgcg accaatcatc
     4921 gtaatgaata tgagaagtgt gatattataa catttcatga ctactgcaag actaaaatta
     4981 acatgacaag tctggtttcc ttggaaaatg tctcggtttc ttttggccaa cgccgcgtcc
     5041 tctctgatgt gtcgctggaa cttaaacctg gaaaaatttt gactttactt gggccaaatg
     5101 gcgcaggtaa gtcgacactg gtacgggtag tgctcgggct ggtaacaccc gatgaagggg
     5161 ttatcaagcg caacggaaaa ctgcgcatcg gctatgtacc gcagaagctg tatctcgaca
     5221 ccacgttgcc actgaccgta aaccgttttt tacgcttacg ccctggtaca cataaagaag
     5281 atattttgcc tgcactgaaa cgtgtccagg ccgggcacct gattaacgca ccgatgcaaa
     5341 agctctctgg tggcgaaacg cagcgtgtac tgttagcgcg agcattgtta aatcgcccgc
     5401 aattattagt gctggatgaa cccactcaag gcgtggatgt aaatggccag gtggcgttat
     5461 atgaccttat tgaccaactg cgtcgtgaac tggattgtgg cgttttaatg gtttctcacg
     5521 atctgcatct ggtgatggca aaaaccgatg aagtgctgtg cctgaatcac cacatttgtt
     5581 gttccggcac accggaagtt gtttccctgc atccggagtt tatttcaatg tttggtcctc
     5641 gtggtgctga acaactgggt atctatcgcc atcatcataa ccatcgtcac gatttacagg
     5701 gacgaattgt tttgcgtcgg ggaaatgatc gctcatgatt gaattattat ttcccggttg
     5761 gttagccggg atcatgctcg cctgtgccgc gggtccgctg ggttcgtttg tagtctggcg
     5821 tcgtatgtct tatttcggtg atacgctggc tcatgcctca ttacttggtg tcgcgtttgg
     5881 tttgttgctg gacgtgaatc cattctatgc ggtgattgcc gttacgctgc tgctggcggg
     5941 cggtctggta tggctggaga agcgtccaca gctggcgatc gacacgttat tagggattat
     6001 ggcgcacagt gccctgtcgc tgggcctggt ggtcgttagt ctgatgtcta atattcgtgt
     6061 tgatttgatg gcttacctgt tcggtgattt gctggcagtg acgccagaag atctcatctc
     6121 tattgcgatt ggcgtggtca tcgtggtggc tattttgttc tggcaatggc gcaatttgct
     6181 gtcgatgacg attagcccgg atctggcgtt tgttgatggt gtgaaattac agcgcgtgaa
     6241 attgttgttg atgctggtga cggcattgac gattggtgta gcgatgaaat ttgtcggtgc
     6301 gttgattatt acttcgttgc tgattattcc tgcagctact gcgcgtcgct ttgcccgtac
     6361 gccggaacag atggctggtg tcgctgtttt ggtggggatg gtggcagtaa ctggcggttt
     6421 aaccttttcc gcgttttacg atacgccggc gggtccgtcg gtagtgctat gtgctgcact
     6481 gttatttatt atcagtatga tgaaaaagca ggccagctaa tctgtcgctg aacacatttg
     6541 tcggatgcgg cgcgagcgcc ttatccgacc tacggttcgc tatctctggt aggccggata
     6601 agacgcgaat agcgtcgcat caggcacact gccaatgtcg gatgcggtgc gagcgaccaa
     6661 tccgacttac ggcatttctg gcggcgttat gccaaagtga ttccacgccc gcgtcgtcgc
     6721 catacgccca cgcggtgtac gctgcaaaaa gccttgctga atcagataag gctccagcac
     6781 atcctcaatg gtttcacgtt cttcgccaat ggctgccgcc aggttatcca gacctaccgg
     6841 cccaccaaag aacttatcga ttaccgccag caacaatttg cggtccatat aatcgaaacc
     6901 ttcagcatcg acattcaaca tatccagcgc ctgagcagcg atatctgccg agatggtgcc
     6961 atcgtgcttc acttcggcga aatcacgcac tcgacgcagc agacggttgg caatacgtgg
     7021 cgtaccgcga gcgcgacgag caacttccag cgcgccgtca tcactcatct caagccccat
     7081 aaagcgtgcg ctgcgactga cgatatattg cagatccggc acctgataaa actccagacg
     7141 ttgcacaata ccaaaacggt cgcgcaacgg tgatgtcagc gaacctgcgc gcgtggttgc
     7201 accaatcagg gtaaacggcg gcaaatcaat tttaatggag cgtgccgccg gaccttcacc
     7261 aatcatgata tccagttggt agtcttccat tgccgggtac agcacttctt caacaactgg
     7321 cgatagacgg tggatctcat caataaacag cacgtcatgc ggttcaaggt tagtgagcat
     7381 cgctgccaga tcgcccgcct tttccagcac cggaccagaa gtcgtgcgta aattaacgcc
     7441 catttcattg gcgacaatgt tggcaagcgt agttttaccc aaccccggag gaccaaaaat
     7501 caacagatga tcgagggcat cgccgcgcag tttcgctgct ttgatgaaaa tctccatctg
     7561 tgaacgaacc tgcggctgac caacatactc ttccagtaat ttagggcgaa tggcgcgatc
     7621 tgccccatct tccggcaaag tggtaccggc agaaatcaga cggtctgctt caatcatcct
     7681 ttacctcata acgcggcgcg tagggcttcg cgaattaatg tttcactgct ggcgtcagtg
     7741 cgagcgattt tgctcaccat gcggcttgct tcttgtggtt tatagcccag cgccaccagc
     7801 gcggcaaccg cttcctgttc agcatcgtcg gttgccgggc tggcaggcga cgtgagcacc
     7861 agatcggcgg ctggcgtaaa gagatcgccg tgcaaacctt taaatcggtc tttcatttcg
     7921 acaattaaac gctcggcggt ttttttacca atgcccggca gtttcaccag tgctcccact
     7981 tcttcacgtt caacggcatt aacgaactgc tgcgctgaca ttccggagag gatcgccagc
     8041 gccaacttcg ggccgacgcc gttggttttg attaactctt taaacaacgt gcgctcttgt
     8101 ttattgttaa aaccgtacag cagttgcgcg tcttcacgca ccacaaagtg ggtgaaaacg
     8161 atcgcttcct gacccgcttc agggagttca taaaaacagg tcatcggcat atgcacttca
     8221 tagcctacgc cgcccacttc aattaacacc agcgggggtt gtttttcaat gatgatgcct
     8281 ctgagtctgc ctatcacatg acgctcctgc gtaatgaatc aaagataatg ctgtatgata
     8341 aaaaaatgct ggatagatat ccagcgaagg atgaagaaaa cttgcgaggt gtctcgatga
     8401 actgaaaaat ggcacagtat aatttattct acaggttata ttggaagcaa atattttaat
     8461 attacatatt cagtgaataa atgcgtaata aaaatataca ttgcgcctcc tgaaaaaaaa
     8521 taaatttttt atgctattac gtatattcgt atctatttca atggaatgac aacgtgaata
     8581 ttaattaccc tgctgaatat gaaattggag atatcgtctt tacatgtata ggtgctgcct
     8641 tatttggtca aatatcagct gcatcaaatt gctggagtaa tcacgtcggg atcattatcg
     8701 gtcataacgg tgaagacttc ctggttgcag aaagccgggt tcccctttca accatcacca
     8761 cgctatctcg ttttattaaa cgttcgtcta atcaacgcta tgctataaag cgattagacg
     8821 ctgggctgac agaacaacaa aaacaacgaa ttgttgaaca ggttccctcc cggctacgca
     8881 aactttacca caccggtttt aaatacgaat cttcgcgcca gttctgttca aaatttgttt
     8941 ttgatattta taaagaagcg ctatgcattc cggtgggtga aatagaaacg tttggagaac
     9001 tgttaaatag caatccgaat gcaaaactca ctttctggaa attctggttc ttaggttcta
     9061 ttccgtggga gcgtaaaacc gtcacgccag ccagtttgtg gcatcatccg ggtctggtgt
     9121 tgattcacgc ggagggagtt gaaacgcctc agcctgaact gaccgaggcg gtataactta
     9181 acgcagtcgc cctctcgcca ggttcagccg cgattcgctc atctgcattg cattctggct
     9241 gacgtgacaa tgggtaatcg cgatagctaa cgcatcggcg gcatccgcct gtggattagc
     9301 gggcagtttc agcaaggtgc ggaccatatg ctgcacctgg cttttttcgg cactaccaat
     9361 acctaccacc gtttgcttta cctgacgtgc cgcgtattca aataccggca attcctgatt
     9421 caccgccgcc acaatcgcca cgccgcgcgc ctgccccagt ttcagagccg agtcggcatt
     9481 tttggccata aagacttgtt caatggcgaa ataatctggc tggaactggg tgatgatttc
     9541 cgtcacgccc gcatagatga gcttcagacg agacggtaaa tcatccactt tggtgcgtat
     9601 gcatccgcta cccaggtagg acagttgcct gcctacctgg cggatgacac catagccgat
     9661 cacgcgcgaa cccgggtcaa tgccgagaat aatagccatc acgcgtctcc gttttgctgt
     9721 ttagcaggcc ccatcagaga gttgctgcaa cctcatcaga gatttcaccg ttatggtaga
     9781 cttcctgcac gtcgtcgcaa tcttccagca tatcgatcag acgcatcagt ttcggtgcgg
     9841 tttctgcatc catatcagct ttggtggacg ggatcatgga aacttccgcg ctgtctgctt
     9901 tcagacctgc cgcttccaga gcgtcgcgca ctttacccat ttcttcccat gcagtgtaga
     9961 cgtcaatcgc gccgtcatca taggtcacaa cgtcttcagc acctgcttcc agtgctgctt
    10021 ccataatggt gtcttcatcg cctttctcga aggagatcac gccttttttg ctgaacagat
    10081 aggcaacgga accatcagta ccgaggttac cgccacattt gctaaatgca tgacgcactt
    10141 cagcaacggt acggttgcgg ttgtcagaca gacattcaat catgattgcc gtgccgccag
    10201 gaccgtaacc ttcgtagatg atggtttcca tgtttgcatc atcatcaccg cccacaccac
    10261 gtgcaattgc gcggttcagt gtgtcacggg tcatgttgtt agacagtgct ttatccaccg
    10321 ccgcacgcag acgcgggtta gcgtccggat cgccaccgcc cagcttagcc gcggttacca
    10381 gctcacgaat gattttagtg aagattttac cgcgcttagc atcctgcgca gctttacgat
    10441 gtctggtgtt ggcccattta ctatgacctg ccataaaaat atctccagat agccctgcct
    10501 gttcaggcag cgttaattac aaactgttca atcgcctgcc ggttgctcca ggacttagtg
    10561 agcgccgcgg cagcaggcgc atcaagccac ttgtaagcca gatgttcagt gaagactacc
    10621 tggcgttcgt gcgggagcgc aagacagaac catgattccg tattacgcgt cacgcccggc
    10681 gcatagcgat gacgtaaatg tgaaaaaatt tcaaactcta ccgtgcgctg acagtcaatt
    10741 aaggtcagtt gttcagcgac aacatcaatg gtgacctctt cctttacttc gcgcatggca
    10801 gcttgcggcg cggtttcacc ctcttccacg ctgccggtta ccgactgcca gaaatcggga
    10861 tcgtcacgcc gctgcaacat cagcacccgt ttcgtatctt gtgcatagat gaccactaag
    10921 atcgaaacgg gacgcttata cactttatcc ttcacgctgc ctcttcgttg actgccttcg
    10981 ctcatcccat tcacatagtt atctatgctc atgggagttc actcagttgc cgccttgatg
    11041 caacgcgaat gatttcgtgt atttgagtca tatcagttat tctcagcctt cttcacaacc
    11101 tgaatgctca gctcagccag tacagtcggg ttagcaaagc tcggtgcttc agtcatcaga
    11161 cacgctgccg ccgtggtttt cgggaaggcg ataacgtcac ggatattgtc ggtgccagtc
    11221 agcagcatgg tcagacggtc aagaccgaat gccagacctg cgtgcggcgg agtaccgtat
    11281 ttcagggcgt cgagcaggaa gccgaatttc tcgcgctgtt cctcttcgtt gatacccaga
    11341 ataccaaaca ccgtctgctg catatcacca ttatggatac gtactgaacc accgcccact
    11401 tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg cattttccgg tgcagctttc
    11461 agttctgcag cagtcatgtc tttcggtgag gtgaacggat ggtgcattgc cgtcaggccg
    11521 ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc acagcggtgc ccatttgctt
    11581 tcgtcggtca gaccaaggtc tttacccact ttcaggcgca gtgcgcccat cgcgtcggca
    11641 acaattttct tgttgtcggc accgaagaaa atcatatcgc catcttgcgc ggcagtacga
    11701 tccaggatgg cttcgatgat ttctgcatta aggaacttcg ctaccgggct gttgatacct
    11761 tccagacctt tcgcgcgttc gttaactttg atgtaagcca gacctttcgc gccgtagatt
    11821 ttaacgaagt taccgtattc gtcgatctgc ttacgggtca gcgatgcgcc acccggaaca
    11881 cgtagagcgg caacacggcc tttcggatcg ttcgccggcc ccgcaaatac tgcaaactca
    11941 acagatttca gcagatcggc aacgtcggtc agttccatcg ggttacgcag atccggttta
    12001 tcagaaccat aacggcgttc tgcttccgca aaggtcatta ccgggaaatc gcccagatcc
    12061 acgcccttca cttccagcca caaatgacgc accagcgctt ccatcacttc acgcacttgc
    12121 ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa attcaggctg acgatcagcg
    12181 cgcaggtctt cgtcacggaa gcatttaacg atctgatagt aacggtcaaa accggacatc
    12241 atcagcagct gtttgaacaa ctgtggggat tgcggcagtg cgtagaattt acctttgtgc
    12301 acacgagaag gcaccaagta gtcacgcgcg ccttccggcg tggctttggt cagcatcgga
    12361 gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc gcaccaggct ggtgatttta
    12421 gcgcgggttt tcaggcgctg cgccatttcc gggcgacgca ggtcgaggta gcggtatttc
    12481 agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg gcagaacatc tgcgcggttg
    12541 atgatagtca gcgaggacgc cagcacttca atttcgccgg tcgccatatc gcggttaata
    12601 tttttttcgt cacgcgcacg tacggtgccc gtaacctgaa tgcagaactc attacgcagt
    12661 tcagaggcca gctttaacgc gtccgcacga tccggatcga aaaatacctg cacgatacct
    12721 tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat cacgacgacg gttgacccaa
    12781 ccacacagag tcacctgctg ccccacgtgg gacaaacgga gctgtccaca atattctgta
    12841 cgcatgagat atcccttaac ttagctgccg gcggatgccc cctgctgcgc aggtgaccaa
    12901 gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag gcggctatta tactggaaat
    12961 tctgccgcac cgtaagagcc tggcccgcgc tgtaacgcct cgttaccact ttatatcggg
    13021 cctgaaatca gactctacgc cagtttgcta taaaggtgtt gcccgaactc ataaaaatta
    13081 acaaaatttg tcgttccgcc atcggctaat cgcattaagg tgtgaggcac gattttgttt
    13141 tttcaggagt cagcatgctt gaacttaatg ctaaaaccac tgcgttggtg gtgatcgatt
    13201 tacaagaagg catcttacct tttgccggtg gtccacatac tgccgatgag gtagttaatc
    13261 gcgccgggaa gctggcggcg aaatttcgcg ccagcggtca gcccgtgttt ctggtgcgag
    13321 ttggctggtc tgccgattac gccgaagcat taaaacagcc ggttgatgcc ccctcccccg
    13381 ctaaagtgtt gcccgaaaac tggtggcaac atccagctgc attaggtgca actgacagtg
    13441 atatcgaaat catcaaacgt cagtggggtg cgttttacgg tacggatctg gagttgcaac
    13501 tgcgccgccg aggtatcgat acaatagtgt tatgtgggat ctcgaccaat atcggtgttg
    13561 aatccaccgc ccgcaatgcc tgggaacttg gctttaatct ggtgattgcc gaagacgcct
    13621 gtagtgccgc cagcgccgag caacacaata acagcattaa tcatatctac ccgcgcatcg
    13681 cccgtgtgcg tagcgtggaa gagatccttc acgcgttatg atttacatcg gtctaccgca
    13741 atggtcgcat cctaaatggg tgcggttggg gatcaccagc cttgaagagt atgcccgcca
    13801 ctttaactgc gtgatacggg atttttaaaa atcactaaag aacgcccaag agcatgtgtt
    13861 ttctttagaa taatcaaagc gttggattgc catgccatat gaaaatcggc aaagttcata
    13921 tgtaacaaag tgtcccaatc atgccccaaa atgccccaca acaaatattt ttgccccatg
    13981 catgccccaa aaaatcactt tccctcactc ttgacactgt ttatccatac agttaaaaat
    14041 aatactgtat aaaaacacag tgtagaggga tttttatgcg tattgaaatc tgcatagcca
    14101 aagagaaaat gactaaaatg ccaaccggtg ctgtggatgc gttaaaggaa gaattaaccc
    14161 gacgcatcag taaacgttat gacgatgtag aggtgatcgt aaaagccacc agcaacgatg
    14221 gcctttctgt tacacgcacc gcagataa
//
LOCUS       DAJTYZ010000103        14114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000103 DAJTYZ010000000
VERSION     DAJTYZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.103"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(104..692)
                     /locus_tag="OO596_002986"
                     /pseudo
     CDS             complement(104..692)
                     /locus_tag="OO596_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(849..3029)
                     /locus_tag="OO596_002987"
     CDS             complement(849..3029)
                     /locus_tag="OO596_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6718788.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            complement(3128..3334)
                     /locus_tag="OO596_002988"
     CDS             complement(3128..3334)
                     /locus_tag="OO596_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718789.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            complement(3359..4069)
                     /locus_tag="OO596_002989"
     CDS             complement(3359..4069)
                     /locus_tag="OO596_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6718790.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            complement(4545..4850)
                     /locus_tag="OO596_002990"
     CDS             complement(4545..4850)
                     /locus_tag="OO596_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718791.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            complement(4997..5212)
                     /locus_tag="OO596_002991"
     CDS             complement(4997..5212)
                     /locus_tag="OO596_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718792.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            complement(5433..5723)
                     /locus_tag="OO596_002992"
     CDS             complement(5433..5723)
                     /locus_tag="OO596_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718793.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            complement(6047..6343)
                     /locus_tag="OO596_002993"
     CDS             complement(6047..6343)
                     /locus_tag="OO596_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718794.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            complement(6343..7296)
                     /locus_tag="OO596_002994"
     CDS             complement(6343..7296)
                     /locus_tag="OO596_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6718795.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEKELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            complement(7679..8341)
                     /locus_tag="OO596_002995"
     CDS             complement(7679..8341)
                     /locus_tag="OO596_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6718796.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCNLPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNGLTIRSPFPSAVVVFKKEPRKHEITQTVCCGD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKCIADALRMPLSTSYRIAQR
                     LS"
     gene            8857..9426
                     /locus_tag="OO596_002996"
     CDS             8857..9426
                     /locus_tag="OO596_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6718797.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            10154..10327
                     /locus_tag="OO596_002997"
     CDS             10154..10327
                     /locus_tag="OO596_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6718798.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(10585..11913)
                     /gene="rutG"
                     /locus_tag="OO596_002998"
     CDS             complement(10585..11913)
                     /gene="rutG"
                     /locus_tag="OO596_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6718799.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(11934..12428)
                     /gene="rutF"
                     /locus_tag="OO596_002999"
     CDS             complement(11934..12428)
                     /gene="rutF"
                     /locus_tag="OO596_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6718800.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(12439..13029)
                     /locus_tag="OO596_003000"
     CDS             complement(12439..13029)
                     /locus_tag="OO596_003000"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6718801.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(13039..13839)
                     /gene="rutD"
                     /locus_tag="OO596_003001"
     CDS             complement(13039..13839)
                     /gene="rutD"
                     /locus_tag="OO596_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6718802.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(13847..>14114)
                     /gene="rutC"
                     /locus_tag="OO596_003002"
     CDS             complement(13847..>14114)
                     /gene="rutC"
                     /locus_tag="OO596_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6718803.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         3866 a         3274 c         2917 g         4057 t
ORIGIN      
        1 ctgagtactt tttaagcttt gaagctactc gaccagcgtt actattagca ttggtgacgt
       61 tggttttttc agcttctgtt aagtctgaat agtccagctt ccaagcctta ttacatttaa
      121 tctgagcatc gagaatatca tttctgatag ctggagcgta accggataca tcgctaatat
      181 tcattaactc tttgcacgat agcataggcg cgttattgcg ttcagtggtc acgttacctg
      241 cattcttctt actggtcaca ccaccgtcat ttgtggtagc cgttttgcca gtggctgctg
      301 aaatatcagt ttgacctacg attgctttaa agttaatttc ctgaaaagtt agctggatac
      361 gaagaccgtt agagatagta acatctttag aggctttaaa gttggtaata atggaatcgt
      421 caattttaat tccatcttta cagataacag aaataatctg cttctggtca cgccagtttt
      481 ccagagtgtc aatgaagttt tctactaatt gaccctgacg agttaataat aagcttcctt
      541 cataattgca tgttctgtgt agtcacctgc atagggctat caaattccat gttttcagtt
      601 gctgataagg ttaagaaggc atctacattg tctcttaagt ggaaatagat gccatctttg
      661 ccactgtaac gaagctgatt agtgtaaatc attaaattct cctttgattt caaaggctta
      721 gtgtatttta atcattttta accaagcctt tgatatatat gtattttctc tgttaagcgt
      781 gagctacgtg aaaaataatt tttggtgtgg gtattaactt tgctttattc acgtagaaca
      841 ccgttaaatc attcttcctc tggatagctt gaacttaaga gcatatcctg cgtgaactgg
      901 tcattagctg attgattgaa agtaaagaaa gatggttcat ctggtcgaac gacaacttga
      961 ccaccaagtg aaactttcac tggctcctga atacgcttac tcaaagcact aatttcatcc
     1021 ctcagaccct gaatatctgc actcgttaat gctgaatctt gcttaatgtt gtttaactct
     1081 ggatgattga atcttgcaat attcattgtg gcattgttaa acaattcacc cattgaacta
     1141 aaaattctac tccatccttc gttaatcata ttcggtttat tagggtcacc agaatacacg
     1201 ggattaaagt cactacccac acctacccca gacggatgca tattagcgtt tttgactact
     1261 tcctgattca taaaatccag tgctttcttc aatgcatata aaccaagagt gacgcctctg
     1321 atttgtggaa tcattgaagc taatgctaac gctgtttcat aaccgtcatc agcataacca
     1381 attgcaagag cactacctac accaccagct acacctttca gttttccacc acctttacca
     1441 gtagctacct gaccagccac ctgattagct cttgtaccta acgttgtgat tgctcttgtc
     1501 aggttcataa cccctgtagt agcactgaat agcgtctttg cagataacgc tatagcaaga
     1561 cctttgacga agttgataaa accatcaccg acttcatcaa gtagttttcg tttagtgtca
     1621 tcaaggctgt aatagaactt cttagtctgt aaaatccagc cttcaatggt gttattgaat
     1681 acgcgaatat catctacacc agctttaagc atgtcgataa gtccaccgat agcatcacca
     1741 gcgatagaag aactcatccc catttcacta agcaggttac tcaggctgga taagaagccg
     1801 tttagctcat cctgtgaacc tgagaagatt ttcaccatcg tatcatccca attgtttgct
     1861 aacttaccta aagctgtctg agtgctctta gtgacttcag cccagccacc cgctttatca
     1921 atcatcaagc ccatctgttt agcgacttta gggagagtat cagcggaaag taccttcccg
     1981 tttttcatgg ctgcatcaag ctctgtagca gtcataccca atgcttcagc gaatagctta
     2041 aatgcaccag ccatacggtc acctaactgc ccacgtaatt cctcagcctg aatcttgccc
     2101 ttgctcgcca tctgctcaaa tgctctcaaa atgccgttta gctcatcgtt agaaagcccc
     2161 attaagcgac tcatagccgt tgcattacca aatagctcct gtgtctgttc ctgtgacata
     2221 gaaccctgag cacctgctgc aaacttagcg tagcttgata gcccttcaga gatattaaga
     2281 cctgtcttgt ctgctaaatt tcgaataaat acctcagctt cagatgcttt atcaccaagt
     2341 acaaacttag cggctctctg tgcggaatct cgtttaagac ctgcattcat aatggccttg
     2401 taagcgttaa gagcacccat cacagagtaa aatgcaccag ataatttaat cgcattgctt
     2461 gcaagattat taaatgcgtc gcttgtattg ttaatgctgg cggtctttgt ctgtgacctg
     2521 agtttattca acgtttcacg aagagattga tttaattttg caagttcagc gttaaacttt
     2581 tcacttccaa tattaccagc cttaccgctt aagctgtaat actgtgcttt aaacatttta
     2641 gccatcagcg gattaatatc accaagctta gacatattca cgctatgcgc tgctgtgatt
     2701 gctctgtttc cgctaattcg tggttgcctt gattgttttg gaggattaac agtgtcatta
     2761 aatctggtgg caaactgaaa tgcttttgtg cttcttgctc tctgctcaat cttgtcaaag
     2821 gtcttattaa acttcctttc aagctgccct aaagaacttg tcagattgct gtcaagttgc
     2881 ttggtatcga ccttaaacgc tctattcaaa ttgcgttcaa ttctttgtgc tgactgcaaa
     2941 gctgcctttt caactctttg caagccctta ataacgtttt cactgaaact aagttcaacg
     3001 ataaaactat caactgtgaa tttgctcatt catgtttcac ctctttaagt gttcatatac
     3061 gaaaaaggga aagactaccg atatcactcg ataatctctc ccgtagattt tcacccaacc
     3121 aatcttgtta cctaaccaga taataattac catcttcacc aacaatctga caatcacttg
     3181 gtaatttatc aagacaccac ttagctattt tcctgattct attcttttgc ttagaggagg
     3241 tgaatgattt gattcgtttt ggtttgttgg gttgttcttt caatcgttca ctaattgctt
     3301 taataagctc atagcgttct gttaaatcaa tcattcacca ccacctttta attattaatt
     3361 aaatagcctt tgcatatttg ctagaaatct tcgtaagctg ctcaatatca gcctgcatag
     3421 caatcttctt agcatcttca tattctttca caagctcttt catagcctga agctctcttg
     3481 agagatacaa cttaacatta tcaagttgct tgttacgtga tgccaagatg ttctgtctag
     3541 caatttcagc gagttcttca tcgctcttat tagtcatgta aataaactga ccagcacctc
     3601 ttgatgcctt aaccatcttc acaattcgat gtccagcagg aagactacta atcacggctg
     3661 cgacgttttc ttgaaaagta ccaaaaccat cattatcttc gtattggtaa tagacaaatg
     3721 gtttctgtga caattcatcc tgaatctcaa attctgacgg ctcaatagct gattgcatca
     3781 aggtaacttt gctgtaagcg tcatctaatc taaggcgctg ttctttcagc agttggtaat
     3841 atttcttttc aagctcaagt aatgcttcac tcttgcaaat atcaggcatc tcttgaggct
     3901 caggagacac aaagatggtt gtttttggtg acatctttag cgattgctca agctcaatac
     3961 gcttgtcatg ctctttacgc tgaatagtga tagctggatg ttcagtctgt ggcgaccagt
     4021 cactgaagct atcaatcact ttgccattaa tgtaaatatc atttgtcatt gcttttcctc
     4081 ttctaagcgg aagtgtttca gtttttaaaa agcacacctg acagcctatc taaacttaga
     4141 agagacttaa aaggcggttt gccaagtatg ctttttaaaa gctgaatagc cctagataat
     4201 ttcttataaa gggccattca taaggtgaag aaattaaaac atctaaagga ttcaaaaagg
     4261 attcaaacga atgaacaaga gtacttttcg agatgacctc tcagactaac ctgaaaggtc
     4321 atgtagcgaa aagcattgca cctttcagaa taactatcat tgatgtagaa tgattaatag
     4381 tttaattcag gaagagttgc tcttccctat taccatcatc tcatgcttaa ggttatttgt
     4441 caacagcttt ttaacagatt ggtaacatca attcttattt accgtattac ctacccagca
     4501 accaacaaat aaccctaaag tcatccagag aataacgagt gtcattacat cacctcgctg
     4561 taagggttta attcattctg ataggtcaag aatacattca gcttcaataa ttgctcaggg
     4621 cttaaatcaa gtcgattgca ctcttcaatg ataaagcgct tattgtatgg gtctgttact
     4681 tccagcttgt gaacatagtc accaagttta atttcgttga ttatatgctt gtcaatatac
     4741 gttgacgctt caaaacaatg catgaaatag atattaaagc ccgacctgtt caggtcaatc
     4801 aaccaattca tggtttcttg gtcaaccata atttgcattg ggtaattcat ttttaaaatc
     4861 cttcctgtat cgcattctaa gacgttttaa cccctttttt agaccactta aggcgattcc
     4921 ctaagttacc ctgattaggg gcttaaaaac gtttctggtg cgtctggtaa agcctgagag
     4981 atatgttttg ctgtttttag ctcaagtctg agactaatag ccccatctgg tgaccgtagt
     5041 cttttaacca gctttgctta accggagtct tcagagaata gttaggggct gacaagtgaa
     5101 gatagatatc atctttgccg tggatacttc cccatccttc gtataactca agcaagtttt
     5161 caaaataacg aaatgcttta ttagtctgaa gttgtactgt gatagttttc atcaagcacc
     5221 ccattcagaa ataagcttaa ttccctgctc aagctgttca gggtcgttat agtcgagtat
     5281 taccagtggc tttaattcgt gtttaagctg gtggatttga gagggtttta atcgtttgtt
     5341 tgctttgagg gtcacatctt caaattcagc atctgctaaa ttctgtctga ttaggtggcg
     5401 gccaagtgtg gaatattgga acctataaca cattaaacct ccaatctatc cgccagttga
     5461 tggaccttag cgcttaacac ctgaatcatt gagcaacctg ctactgcgga ctgtgctttg
     5521 agtagttcca gttggcccgt tgtgattctg atggccttag aaccctctgc gacgccctta
     5581 acgttatcga aagggctaag gtttaccgac tcttttaaaa tctccccaac aagcgctgtg
     5641 tgcttcatat cgggatattt ggctttgatt gctgccagtc gttcaaatgc ctctggcaaa
     5701 aggcgcgtaa ctgtgtgagt cattgtaaaa atctccttaa atctcttact catactacaa
     5761 tcttaacatc tatttaacat attgtcagta cctcatttac atgatactat ggttgtaggg
     5821 gctgatacag gtgctgtttc ctttcacaaa aggaaaatta atgcatgatt ttttcacagc
     5881 aattattgaa tctcatcgtt ttagctcgtt gggctttgcg gtatttattg tgctattggt
     5941 gttttgcgtt atgaatcgag tcattacaaa aaagacaaag cttacaattc gacttaaact
     6001 tcttcgtgtt gagatagaga tttcagtaaa taaatagctg gtggccttac cggcactttc
     6061 ccttgataat attggtggcc ctcagattac cggggtcacc gtcttttaat cgtagctggt
     6121 ggactgaatc ccataaatcg taatgataca gcaccgcaaa cttcaaagcg tcaatcttta
     6181 aatctgtcac atcatcgacg aaataataat ccctcccttc tttcttcggt cgaatccggt
     6241 aaacttcccc gtgagtatct ttcatcaagt aaccttcacg agaaatgctg taacgtctga
     6301 gtaaatagtc acggtatttt ctctcgccat tataagtttt cattaaaaca ttcctccacc
     6361 ataaacacgt ctgactttaa ctttttcttt ctttggctcg gtgtgagttt tcttttttgg
     6421 tgtaaaagta aaatcatcgt cactatcatc aaagtcatcg aggtcactaa ccttgttctt
     6481 tagcttcgca tttctgaatc tttctttaat ttctgcttta cgctgttcac gtttcaattc
     6541 cttttcaggg tcttcttcat catcaaggtc aacatccggt aattcatcat cagtggcggg
     6601 gcactgtgtg tttttatggt cactactagc aatcacatca gagattttat cttccttttt
     6661 aacgacctct ttagcagctt cactatcaag gtcatctaag tcagatgttt taacagtctc
     6721 tttaatagcc tcttcaatac caagagaatc acattcatgt gaatcacaga gcgaagctcc
     6781 cttcttaaaa tattcttctt caaagtcatc ttctttattg ttattaataa gttctttctt
     6841 gttattactg ttattatacg tgtcaaagat tttgactgac tgagtaaaaa attttgactc
     6901 agtgggtaaa gaattttgac tcagtgggtc atctttcttg actgactgag taactttaga
     6961 ctccagcttt gcctttctgg cttccttagc tgtaagctct gttaaaacct ccggtacttc
     7021 atcaagtgaa taaactttaa taacacactg attgcttgca acgttagttt tcttgattaa
     7081 accatgtgcc tcaagttgtt tcatacatgc aatcacggat gttcttgtga ttccagcata
     7141 ctgaccaaga tgagcatatg aaggggtata atattgcccc ttatcgtgat aaaacttttg
     7201 ttcattcaac atgacagtgt aaataatacg gtcggcagct ttaacgccat tagtcatctg
     7261 ataaatacgg tcatcaaaca tgtggaattt ttccattata ttttcctttt taaaaataaa
     7321 acaaagaaag ggctgaccac gtatagtgac caaccccaga gggagtgaat taattagaag
     7381 atggactgat aaaggaactc agaagcctga gcgattacgc gagtattaac agcaaaagga
     7441 aaagccttga ttgaataaga ggccgtgaaa atctgtcaat accctgacgc ctcccaacgt
     7501 tgtaacctct taatgttctg agttctttta tcaccccata taaaaactat aacagatgtg
     7561 attataaatt gcaacaatta tttcacagaa taattactga ttggtcattt attgttcaca
     7621 gggatgcttc ttaatatctg aatcacctat cttgtaaatg accatattta tgagaattct
     7681 agctcaacct ttgagcaatc ctgtatgacg tgctcagagg cattctgaga gcatctgcaa
     7741 tgcacttaat gggttttcct tgcctgtata gttttatgaa gctgtcgagc gtctcaggtg
     7801 ggtaaatggt gggtcttcct ctcaagcctc ctctgttaat gatggttaac ggtaatagcg
     7861 tgtcaccaca gcaaaccgtc tgtgtaattt cgtgcttacg tggttctttt ttaaaaacaa
     7921 cgactgcact aggaaacggt gaacgaatag taagcccgtt atggcctgct ggtggaaggt
     7981 atttaactgc acgatgaaga atacggattt catgacagtg gttattgctg taaatgtgcc
     8041 taagccagta gctggtgttg gctctatgcg gtatcaggaa agctactaaa tccgcttcag
     8101 acgcttttaa gaggaacgac ggaatatcgc tgtacggcag gttacagaag accttttcac
     8161 taacccatga ttgacggctt atatcattgg tgaatcgtgg taacagcgtg tcatgctcag
     8221 ttgcagcacc atctagcgta aaaccaaatt cacggtgtaa gttcttaaat aacaacaatg
     8281 gagttctcca gtaatcagaa cccccatgat gtttcccgat taaaatatgc tcaattgaca
     8341 taagaaataa aaaattttat tgcaatcact cttgacaagg tttactctct tttaagataa
     8401 gagatactta acaggctttc taaacggata tcctttctgg aatatctatc tcttatctta
     8461 aaagtcttta cagatacttt aaagatatct gtattaaatc tgtacagtaa cttttcagaa
     8521 tctgaaaatt tttataaaat cgtagtagcc cctcttcaaa ttccccttct aaaaatcttc
     8581 gaaaatatct gacagatgtc tgtagctgct aagtacccgt cacaccagct atgtgacccc
     8641 ctttgcaaaa aagccaaata tcattgactt tctcggtgaa ttgctataaa ggcatctatc
     8701 taggtcttta actgtataaa tacacatgac aacaaacgcc cgttaagtgt ctatataaaa
     8761 aggtagctgt agctatagac aacaattgtg ataattgatt taatggctat agacaagcaa
     8821 gacagacctt tgttgactat atagagagat aagaacatgc gtacattcct ttatgctcgt
     8881 gtatcagttg atgacctcac cacacagaac caagtagaag ctgttaaacg tgctggttat
     8941 gaagtcagac cttcacgagt aattgaggaa gttattagtg gggctacacc agctatggac
     9001 agaccacaat ttgtaaagct ggtggataag ctggaagaag gtgattgtct tgtagtgacc
     9061 aagattgacc gcttaggaag ggataacatt gacgtacaga agaccgtcac aatgttactt
     9121 ggtatgggtg ttaagctggt ctgtctagat ttgcctgtcc aagacctttc aagtgctgaa
     9181 ggtaaactta ttcttcaact attcagtacc tttgcagagt ttgaacttaa ccgcatcaga
     9241 gagcgaacca gagagggctt agaacgagct aaagcacaag gtaaggtgtt gggtcgtcct
     9301 gtagctaaaa acactacaga ggccgtttta gagcataagg ctaagggatt gtcacaaagt
     9361 caggttgcaa agcttctgaa tctctctgtg agaacggtta gccgtcactg gacaaagaca
     9421 ctgtaaaaaa aacttagtag catcttcata gcctccttca tggggacttt tttgtgtctg
     9481 taacatatgg tgaccaaaca tctatatatc gttatatagc cctgtatata gcactatata
     9541 tagtgtctgg agagggacga ctaacaccct gcatagttcc ctgaaaaggg gcttcacaga
     9601 ttctctatat atttcaataa gttaggataa aaagggacta taccgatact gcatagaatc
     9661 tgtgtaggat tgcatagtca taggggttgg aacaagccca actacacctg ataaagcctg
     9721 ttattaacag gtagttgaat gtacgtgttt agctatgtaa aagcaccaga actttagcca
     9781 tttctaacca ctcctcgtgt tatctctatt ccgtagcgat tcgctaccac ttatttaaag
     9841 ataagacgtc cttttcagag tgcaaatttc acaaccactt atttgattta taacaacttt
     9901 cacaagcacg taattttgtc gcaaaatgac acatttttat ctcatcgcgt ttttttaatc
     9961 ataagagcgg cttatggata attattggag atgatatcta ttctcgctaa gaagctgttg
    10021 caggatatta ccaaacgcgg gtctacccgc gtcagttcac taagcttagt cccacgtagc
    10081 gaaaatatgg cagccgccat acgccgcgtt aattctatgc aatatgatgt ctatacccag
    10141 acggaggtca gtaatggcaa accatcgagg cggttccggc aattttgcag aagaccgcga
    10201 aagagcatca gaagcaggta aaaaaggtgg acagcacagc gggggtaatt tcaagaatga
    10261 cccgcagcgc gcatctgaag caggtaaaaa aggtggcaag agcagtcacg gcaaaagcga
    10321 caactagccg ggctaatcaa tgacgaatgc atttttgtct gtagctcgtc aaaaagccat
    10381 caccgccggt tacccggtgg ttgatactga tgacaaatgt aagcttgcct gatgcgcgat
    10441 gcttatcagg cctaccagaa gattgcaata tattgaattt gcactgtttt gtaggccgga
    10501 taaggcgttt acgccgcatc cggcatgaac aatgcgtacg ttgtcaacaa tctgcaccgc
    10561 cgggtaaccc cggcggtttt ctgtttatgg ctcctgatga acaacttctg gcggtggaac
    10621 gtcaaccaac cttctactta acaacgcatt gagtaaaatc gcgccaaagg tggctgtacc
    10681 gatccctccc aacgtaaaac cgcccagcgt gagagcaaaa tcacccgcgc ccagcactaa
    10741 ggttactgcg accataatca aattaccgtt ctggcttaaa tcgacacggt tttgtaccca
    10801 gatccttgcg cctgcgacgg caatcagccc gaacacaaca attgatgcac caccaatcac
    10861 cgcggccgga atggtatgga tcagcgcgcc aaatttcggc gaaaagccca acagcatggc
    10921 gatgacggca gcagcaacaa acaccagcgt cgagtagact tttgtcacgg ccatcacacc
    10981 gatattttca gcataggtgg tgacgccgct accgccgaca gagccggaaa gcatcgttgc
    11041 cagaccatca ccgacgaatg ctcgccccat atacgggtcc atattgcgtc cggtcatccc
    11101 ggcgaccgcc ttgagatgac ctaagttttc cgccaccaga atcaccgcca cgggcgcaat
    11161 cagcatcatt gcctgactat taaaagcagg agtggaaaaa tgtggcaaac cgaaccaggc
    11221 agcatggctg acgagagtaa aatcgacggc ttttcccagc cctaaaacgt tggtcatcac
    11281 gccatacagc agacaggcga caattaaacc tacgagaatc aataaccgct ggatcatgcc
    11341 acgggtaaac accgccacca gcccaataca cagcaccgtc attaccgcca tccagctatc
    11401 aaaggctgaa gccgatacac ttttcactgc gataggcgct aagttcaggc caatcgccat
    11461 caccaccgca cccgtcacca ccggcggcat tagtcgttca atccagcgtg taccaatttt
    11521 catcaccacc aggccaatga cggtataaac cagcccacag gcgataatcc cgcccagtgc
    11581 gatgctgata ttcgggttaa tgccctgacc gttaaagccc gtcgcggcaa tcaccacgcc
    11641 gacaaaagcc gcgctggagc cgagataact ggggacgcgc ccgccggtaa taaagaaaaa
    11701 cagtaacgtg ccgatccccg acattaaaat ggaaagattg ggatccagcc ccatcagaat
    11761 cggcattaac accgtcgcgc caaacatcgc caccgcgtgt tgaacgccca ttactgccgt
    11821 ctgagcaaac ggcaatcgtt catccggcgc gaccacgccg ctctctgtag aggtcgattt
    11881 taactgccag tgaggaaaac cgaacattgc catcagctgt ctccttaagg aggttaacaa
    11941 gcagggcgca tcagcgcgtg ataactgcga tcgaaccaca ccagcccgta gggtgtggtg
    12001 tgacgatgaa tcgcttcgat ggcgcaaaac agaatgtcgt gggtgccgac gctcaccacc
    12061 tggctgatgc ggcagtcaaa cgaaaccaga gcctcttcca gttgcgggca tccggtcaca
    12121 cccgtctgcc agcgggcggc ggcaaagcgg tgttccatgg gcgttttgcc gccaaaaaga
    12181 tttgaaagcg gctcctgccc ggcgctcagt gtatttacac acagcgttcg attttcattg
    12241 aataccggcc agacggacgc cccacgattc aggcacacca gtaatgtggg cggcgtgtcg
    12301 gtcacgctgc agacggcgct ggcggtgaac ccggcgcgcc cggatggacc gtccgtggtg
    12361 atgatattga ccgccgcgcc catacaggac atcgcatcgc gaaaggtttg ttgatcaact
    12421 atgttcatag tttgctcctt acaacagccc gcaggcttct tcaaaggaca gacgtggcag
    12481 gcgcgcaaaa agtttgctgc tatcgccata gccgatatta atcagcagat tgctcttcag
    12541 cgtgctgccc gcaaaaaagg cgtcgtcaac gtgttgacgg tcaaaacccg acatcgggcc
    12601 ggtatccagt cccagcgcac ggcaagcgac gatcagatag gccgcctgca tggagctgtt
    12661 gcgaaacgct gtttcttcgg caagttgtgg gctggaggta aaccaactgc gggcatcacc
    12721 gtggggaaac agtagtggta accgttcata aaattcactg tcccaggcaa cgatagcggt
    12781 gacgggcgcg gtcagggttt tttgtagatt gccgctggaa agcgccgggt gcagacgttc
    12841 ttttccttct acagtgcgga taaacacgat ccgtgccgga gaacagttag ctgatgtcgg
    12901 cccccatttt atcggggcat aaagctcccg taacgtctca tcgctgacgg gtgtctcccg
    12961 ccagccgttg tgagtgcggg catcggtgaa cagggtgcta agcgcacctg ggctaacggc
    13021 ttcgttcata gcaattcctt acagggcggc ttcacggtga tgtaacaggc tggcaagccc
    13081 gttgagtaac agagcattaa acgtttcggg gtcggtcacg ttgcaggcgt gtccgccata
    13141 gcgcatcacc attttctggc tatcgggcag ggcggcatga agctcactgg aacatgctga
    13201 tggcaccagc agatcatcac tggcgcagat gatttgcacc gggcagcgga tgcgatccgc
    13261 atggtgacta aagtcagcgc gtttgagggc gttaagtcga cgcagtaaat tatttttgcc
    13321 ctgaaaatgc gccagtgcca gcgcgtcttc tgcctcaagg cgaggtgcgc gggccgccat
    13381 ccagtcggcg ggatagagga acaacggctg cgcttccacc catgcctgcg cgccgccgct
    13441 atacagtaat cgttcgcgaa cctgaaaaca gcggcgcgta tgggcgttta ttcgtagcca
    13501 gccgttaacg ctgatcagca cagttaccga cgcgggataa tccagcgcca gctgcattcc
    13561 caccagcgca ccgagcgcat ggccgaccac cgcgtaatgc tcaatccctg cggctaccag
    13621 cgcctgatgc agttccgctg ccatctgggc gatactgtaa tcttctgcca gcgtgtcggg
    13681 attattgccg gtgccgcgct ggtcgtaaca gactacctga tactcctgct ccagcaccgc
    13741 cagttgcggt aaccagtaac tgccgctacc gccaagaccc gaaatcaaca ccactacggg
    13801 cgcatcagca taagggggag gtgagagtga aagtttcatc gcggcctcac ttggcgatat
    13861 gcgcaattgt ggcgatttcc accagcgcgt caggttttac cagtccgcac tgaatgcaga
    13921 atcgcgccgg tttatcaccc ggaaaaaact cggcgtagat ttcgttaatc gcggcgtaat
    13981 ttttccagtc ggtaataaag atgctgttga aggtcacatc cgccatcgtg ccacccgccg
    14041 tctcgatcac cttgcggata gtttccagaa cgtggcgggt ttgcgccttt gggtcatcgg
    14101 caaacagcac gtta
//
LOCUS       DAJTYZ010000104        14091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000104 DAJTYZ010000000
VERSION     DAJTYZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.104"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="OO596_003003"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="OO596_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6718804.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="OO596_003004"
     CDS             2273..2662
                     /locus_tag="OO596_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6718805.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="OO596_003005"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="OO596_003005"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6718806.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OO596_003006"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OO596_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6718807.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="OO596_003007"
     CDS             complement(4321..4521)
                     /locus_tag="OO596_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6718808.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="OO596_003008"
     CDS             4687..5232
                     /locus_tag="OO596_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6718809.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="OO596_003009"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="OO596_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="OO596_003010"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="OO596_003010"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6718810.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="OO596_003011"
     CDS             complement(6529..7353)
                     /locus_tag="OO596_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6718811.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OO596_003012"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OO596_003012"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6718812.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OO596_003013"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OO596_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6718813.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OO596_003014"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OO596_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6718814.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OO596_003015"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OO596_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6718815.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OO596_003016"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OO596_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6718816.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OO596_003017"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OO596_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6718817.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="OO596_003018"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="OO596_003018"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6718818.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3433 a         3562 c         3737 g         3359 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg a
//
LOCUS       DAJTYZ010000105        14008 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000105 DAJTYZ010000000
VERSION     DAJTYZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14008
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.105"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1272)
                     /gene="hprS"
                     /locus_tag="OO596_003019"
     CDS             complement(<1..1272)
                     /gene="hprS"
                     /locus_tag="OO596_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6718819.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKA"
     gene            complement(1272..1943)
                     /gene="hprR"
                     /locus_tag="OO596_003020"
     CDS             complement(1272..1943)
                     /gene="hprR"
                     /locus_tag="OO596_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6718820.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2076..2489
                     /gene="hiuH"
                     /locus_tag="OO596_003021"
     CDS             2076..2489
                     /gene="hiuH"
                     /locus_tag="OO596_003021"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6718821.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2598..3602
                     /gene="msrP"
                     /locus_tag="OO596_003022"
     CDS             2598..3602
                     /gene="msrP"
                     /locus_tag="OO596_003022"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR6718822.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3603..4238
                     /gene="msrQ"
                     /locus_tag="OO596_003023"
     CDS             3603..4238
                     /gene="msrQ"
                     /locus_tag="OO596_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6718823.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4495..5145
                     /gene="zinT"
                     /locus_tag="OO596_003024"
     CDS             4495..5145
                     /gene="zinT"
                     /locus_tag="OO596_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6718824.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5488..6018
                     /locus_tag="OO596_003025"
                     /pseudo
     CDS             5488..6018
                     /locus_tag="OO596_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6588..6677)
                     /locus_tag="OO596_003026"
     tRNA            complement(6588..6677)
                     /locus_tag="OO596_003026"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6641..6643),aa:Ser,seq:cga)
     gene            6771..7568
                     /gene="mtfA"
                     /locus_tag="OO596_003027"
     CDS             6771..7568
                     /gene="mtfA"
                     /locus_tag="OO596_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6718825.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7669..7744
                     /locus_tag="OO596_003028"
     tRNA            7669..7744
                     /locus_tag="OO596_003028"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7702..7704,aa:Asn,seq:gtt)
     gene            8058..14008
                     /gene="yeeJ"
                     /locus_tag="OO596_003029"
                     /pseudo
     CDS             8058..14008
                     /gene="yeeJ"
                     /locus_tag="OO596_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3963 a         3332 c         3152 g         3561 t
ORIGIN      
        1 cgctttgact aaagaaagac caagtccctg acctacggaa tgacgcgaat tatctccccg
       61 ccaaaatcta cggaagagtt tttcaggctc atgaattttc gttccaggac tggcgacatc
      121 aatattaaga tagccgttgg tatcaagaaa actggttata tgaatacgcg atttttctgg
      181 tgaatatcta atggcattaa caataagatt cgataacatt cgttgcagta aaattttatc
      241 cgcaaagatt tgctgattgc actcgacctt aaagcaaatc tctttctcgt ctgaaaggta
      301 ttcaagataa tccaacaaat tttcgacttc cttattgaga gaaagcgagt ccagtttcac
      361 caaaacattg tttttatctg cccgggcaag aaacagtatg ttctctgtta accgcgaaat
      421 attttccagc tcttcaatgt taccggcaat tgttttttga tattcagcga tacttctggt
      481 ttgactgagc gtaacctgat tctgacccag taatgcatta atgggcgttc taagttcatg
      541 agcgagatcg tcagcaaatt gacttagacg ctcaaaatct ttgactaaag catgatgcat
      601 tttattcaac gcctgcccaa gaggttttag ttctctcggt aatgcgctaa cctcaacagg
      661 ctcccggcta tcgttataat tcagcgcttc cgttacacca ctcaactttt tgatctctcg
      721 taatcccgtt ctgattaaca gcggacttaa tactgagcaa agtacaatgg cgacaatgca
      781 aattataatg ctattaattt tatactgttc aagcatgtta tgtctggctg aagccaattt
      841 agccacagta accgttaatg atggcgaaac ttcatcaata ttaattcgta aagcatcaat
      901 ctcagtatca ttaataatgc ttctgtaaat gccagctgcg ctgattgtct cactagcagg
      961 tatgttattt aacatgtcat cactgacatt tgtccggtta acaattttat tgatgccatc
     1021 accatgaatg atcaagatat cctgactaac atccatcatc cggttaaagt atacaggtaa
     1081 cgtatctgga tttaccccat caattaacaa ctgcttgatc tgtgctgtcc ggttaatgag
     1141 tgttgtatca tcgcgccatt tcaactcact tgccaggcca ttatagagag tccagacaat
     1201 tccagcgcca gcaacagaca gcagcaatat aaaaagcaag gttaaacgga cggttataga
     1261 taatcttttc attatttttt taccgctacg aatgaatagc ccatcccccg gattgtggcg
     1321 attagctttt caggaaaagg atcatcaact tttgcgcgga gcctgcgaat ggcgacatcc
     1381 accgtattgg tatcactatc aaagttgatt ccccaaattt cactcgcaat aaccgttctg
     1441 ggtataattt cgccagctct ggaggccagt agccaaagta actgaaactc cttgcgcgtc
     1501 agtgtaatac tgatattgtt cctgctcaca ctttgactta cagagtccat tcttaagcca
     1561 ctgatttcta atgttgaatt caaagcgtga tgttgcctta attgtgcccg aacccttgcc
     1621 agcaactcag aaaatgaaaa aggttttacc agataatcat ttgctccact gtccagccct
     1681 ctgactctgt catcgacaga atcccttgca gtaaggcaaa taacaggggt ttgctttgct
     1741 gttcttaacg tttgtaagat ctgccagcca tccatacccg gaagcataat atccagaatg
     1801 atcaatgcat aatcatcctt cagcgcaaga taaagcccat ctctgccatc agaaacggca
     1861 tcaatgacat aacccgcttc ggaaagcccc tgcgttaccc actcctgggt cctttgatta
     1921 tcttcaataa gtaaaatctt catgaaaata atatgccata aatcatatgt tatagaaaca
     1981 gcctagttca ttacaaaatt gtaatgctgc tgtaaggtta ccctggccgc tttttcgcta
     2041 tcctcaaaac tcattcacat gacaaggata taaacatgtt aaagcgttat ttagtactct
     2101 ccgtagtaac ggcagcattt tcattacctt ctttggttta tgccgcacaa caaaacattc
     2161 ttagcgtgca cattttgaac cagcaaaccg gaaaacccgc tgccgacgtg acagtcactc
     2221 ttgaaaagaa agcggacaac ggctggttac aacttaatac cgccaaaaca gataaggatg
     2281 gacgaattaa ggcactgtgg cccgagcaaa ctgcaactac gggcgattac cgtgtcgtat
     2341 ttaaaaccgg ggactatttc aagaaacaaa atcttgaaag tttcttccct gagatccccg
     2401 ttgaatttca tattaataaa gtgaacgagc attatcatgt gcctttatta cttagccaat
     2461 atgggtattc aacctatcgt ggcagttaat gcataaatat gtaaaagcag atctctgcta
     2521 ctgttaggga ggacactccc tgacagatta acagtaaacg gctcttgctg gctaacgaca
     2581 aaaaagtgtg atggcttatg aaaaagaatc aatttttaaa agaatcagat gttacggccg
     2641 agtcggtatt ctttatgaag cgtcgacagg tgttaaaagc actgggcatc agcgcagctg
     2701 cactttcttt gcctcacgct gcgcatgccg atctgcttag ctggtttaaa gggaacgatc
     2761 gcccgcccgc ccccgccgga aaaccgctgg agttcagcaa gcctaccgcc tggcaaaata
     2821 atctgccact gacgccagta gataaagtct ccggttataa caacttctat gaattcgggc
     2881 tggataaagc cgatcccgcc gctaatgctg gtagcctgaa aaccgatcca tggacactga
     2941 aaatcagcgg cgaagtggca aaaccattga ccctcgatca cgatgattta acccgtcgct
     3001 tcccgctgga agagcgtatt tatcgtatgc gctgcgtgga ggcatggtcg atggtggtgc
     3061 cgtggattgg ttttccgctg cacaaattgc tggcgcttgc cgaacccacc agcaatgcga
     3121 agtatgtcgc tttcgaaaca atttatgcac cggaacaaat gccaggccag caggaccgct
     3181 ttatcggcgg cgggctgaaa tatccttatg tcgaaggatt gcgtctcgac gaagcaatgc
     3241 atccgctcac actgatgacc gtgggtgttt atggcaaggc gttaccgcca caaaatggcg
     3301 cgccggtacg actgattgtg ccgtggaaat atggctttaa agggattaaa tcgatagtca
     3361 gtattaagct gacccgcgag cgtccgccaa ccacctggaa tctggcagcg cctgacgaat
     3421 acggttttta cgccaacgtt aatccgcatg ttgatcaccc gcgctggtca caggctaccg
     3481 aacgatttat tggttcaggc ggcatcctcg atgttcagcg ccagccaacg ctactgttta
     3541 atggttacgc cgaccaggtg gcatcgctgt atcgtggcct ggatttgcgg gagaatttct
     3601 gaatgcgtct gacagcaaaa caggtgacat ggctgaaagt tagcctgcat cttgccggat
     3661 tgttgccgtt tctctggctg gtctgggcga tcaatcacgg tggactgggt gccgatccgg
     3721 tgaaagatat tcagcatttt accggtcgca ctgcactgaa atttttgctg gcgaccttgt
     3781 taatcacacc tctggcacgc tacgcaaaac agccgttatt gatacgcact cgccgcctgt
     3841 taggcttatg gtgctttgcc tgggcgacac tgcatttaac cagttacgca ttgctggagc
     3901 tgggcgtgaa taatctggcg ttattaggca aggagttaat tacccggcct tatttaacgt
     3961 taggaattat cagctgggtt attttgcttg ctttagcgtt tacttcaacc caggcgatgc
     4021 agcgaaaact cggcaagcac tggcaacagt tgcataattt cgtctatctt gtcgcgatcc
     4081 tggccccaat acattatctg tggtcagtga agattatttc accgcagccg ctcatctacg
     4141 ctgggctggc tgtactgctt ttagccttac ggtataagaa gttgcgttcc ctttttaagc
     4201 ggttacgcaa gcaggttcac aacaaattat ctgtataact tcatattgcc gacaaagtac
     4261 gcgttatccc tgccggatgt ggcgcgagcg ccctgtccgg cctgcgcaga tcacatcagg
     4321 ttatgtttcg tttgtcatca acctcaaatt atcaacataa ttcattttat ttcaataaga
     4381 tagataagta cacctgagaa aactatgctc tcatctccta agagttgttg cattttgcca
     4441 tatgttacaa tataacatta cacatcatat acaatgactc tggaggaaac tgttttggca
     4501 attcgtcttc acaaactggc tgttgcttta ggtgtcttta ttgttagcgc tcctgccttt
     4561 tcgcatggtc atcactcaca cggcaaaccc ttaacagagg tcgaacaaaa agctgctaat
     4621 ggtgtttttg atgatgccaa tgtacaaaac cgaacgctca gtgactggga tggagtctgg
     4681 caatccgttt atcctttact gcaaagtggc aaacttgacc ccgtctttca gaagaaagcg
     4741 gatgcagata aaattaaaac atttgctgaa attaaagatt attatcacaa aggttatgca
     4801 acagatatcg agatgattgg cattgaggac ggcattgttg aattccatag aaataatgaa
     4861 acaacatcct gtaaatatga ttacgatgga tacaaaatac tcacctataa atcaggcaag
     4921 aaaggcgttc gttacttatt cgaatgtaaa gatcctgaaa gcaaagcccc taaatatata
     4981 caatttagcg atcatataat tgcaccacga aaatcatctc attttcacat ttttatgggt
     5041 aatgattcac agcaattttt attaaatgaa atggaaaact ggccaacgta ttatccatat
     5101 cagttgagta gcgaagaagt ggtcgaggaa atgatgtctc attgagtatt ctcatgataa
     5161 cgcctcgatg ccgctttagt aagtcatcat aactgccact ggtccatcca caaacgccac
     5221 tgaacgcaag ctagctacag acacgctcat cactatgacg tgtctgtata ttaataagct
     5281 aacccgcatt gagttaacca ataacggatt ccatacacaa tacggccaac agagaaagat
     5341 acctgtgctc acgccattgc ttatattggc tggttacaat gtgcactatc aattttttaa
     5401 ataacaaatt cttaaaacca catctgtata aggaattttt aaggttcgtg ggatagcttg
     5461 actgtaaaaa ttacaggagc tacaaaaatg aaccgattct caaaaactca aatttattta
     5521 cattggataa cgctgctttt cgttgcaata acctatgccg cgatggaact ccgtggctgg
     5581 tttcctaaag gtagtagtac ttatctgctg atgcgagaaa cacattacaa tgcgggtata
     5641 ttcgtttggg tgttaatgtt ttcacgcctg attataaaac accgttatag tgatccttct
     5701 attgtaccac cgccacctgc ctggcaaatg aaagcggctt cgctaatgca catcatgctt
     5761 tatataacct tccttgcact tcctctgctg gggattgctt tgatggctta cagtggaaaa
     5821 tcgtggagtt tccttggttt caatgtgtct ccctttgtta ccccaaacag cgaaattaaa
     5881 gcactgataa aaaatattca cgaaacctag gcaaatatag gttacttttt aatcgcagct
     5941 cacgctggcg cagcactctt tcatcactac attcagaaag ataatactct gttacgaatg
     6001 atgcatcgcc gcaaataagt cgatcccctg acaaggaaat gtgaaccatg tacatcaacg
     6061 cgtcataaat gtttacgcaa gtgcacaatg catcctttct actatcaaca aggaggagca
     6121 gaaactgtgt aaatcgtaca atggtaagac ttatcaaaaa tacaggttaa ttttggtatt
     6181 taaacctttt ttacttgtta gtgtgataca aaggctttca aaaaagctgg gaaattcaac
     6241 aactcttgga aattatggac tttgtcccgc ggctttaatc tgacggccgc gttccttttt
     6301 tgactcaatt attgacccct tctctacgca acttcagttc ccaccaccaa ctttgcggca
     6361 gtttcatagg atgaatgtct aaaagaataa tggtgagcga taagaaaacg actgaataac
     6421 tgcacatttt cgctcgaaag cttcctgtca gatccatagc gaatcaagtg ctgaatgtca
     6481 cagtatcgaa cagaaaacag tgacgatcta acccttcaag aatattctac aattgttctg
     6541 tttaggaaaa gcaaggcggg aagtcaggag ataagtcatt gataaagtgg cggagagagg
     6601 gggatttgaa cccccggtag agttgcccct actccggttt tcgagaccgg tccgttcagc
     6661 cgctccggca tctctccgtt cagatggttg ccatgatgcc aggaaatttg gcattttaac
     6721 agtccctgtt cgtgcaattt tgttcaagtg acgagtttgc gagcaaaacg atgattaagt
     6781 ggccctggaa agtacaagaa tcagcacatc aaactgccct tccctggcag gaagcactat
     6841 cgatccccct tttaacgggt ctgacggaac aggaacaaag caaattagtc actcttgccg
     6901 aacgtttttt acagcaaaag cggcttgttc ctttacaggg ctttgaactg gattcattaa
     6961 gaagctgccg gatagcactt ctattttgcc tacccgttct ggagttagga ctggaatggc
     7021 tggatggttt tcatgaagtc ttaatttatc ctgcgccatt tgtggtcgat gatgaatggg
     7081 aagacgatat cggtctggtg cataaccaac gtattgttca gtcaggtcag agctggcagc
     7141 aagggcctat cgttttgaac tggttggata tacaagattc ttttgatgca tctggtttta
     7201 acctgattat tcatgaagtc gctcataagc tggacacccg taacggcgat cgcgccagcg
     7261 gagttccctt tattccgttg cgtgaggttg ctggctggga acacgatctt catgctgcaa
     7321 tgaacaacat tcaggaagaa atcgaattgg ttggcgagaa tgcggcgagc attgatgctt
     7381 atgcagccag cgatcctgct gaatgttttg ccgtactttc tgaatatttc tttagcgccc
     7441 cagaactttt tgctcctcgt ttcccttcat tgtggcaacg tttctgccaa ttttatcaac
     7501 aagatccttt gcagagactg catcacgcta atgatacaga ctcgttttcg gcgacgaatg
     7561 ttcattaatt aacaactttg cagattaatt aaccaattga aatgacttat gaaatttagt
     7621 gttgacagac aaggtaccgc taagtaatat gtgccccgtt cacacgattc ctctgtagtt
     7681 cagtcggtag aacggcggac tgttaatccg tatgtcactg gttcgagtcc agtcagagga
     7741 gccaaattct aaaaattcgc ttttttagcg caatgtcact gaccttagtt gaacattgtt
     7801 ttttaacgga tagcgggttt ttaacacctt aagcgccctc gacctttatg gttgagggcg
     7861 ttttgctatg aacgccatca ccattttccc ctcgattata aaacttgagt tattcagtag
     7921 tctcccctct tgcaactcac acccaaaact gcctaacgaa aagttattaa ttttcaatcg
     7981 tattgctatc agtatttaca ttttttcgct gtgctagaaa gggcgcattt atgttagctc
     8041 gttcagggaa ggtaagcatg gctacgaaga agagaagtgg agaagaaata aatgaccgac
     8101 aaatattatg cgggatggga attaaactac gccgcttaac tgcgggtatc tgtctgataa
     8161 ctcaacttgc gttccctatg gctgcggcag cacaaggtgt ggtaaacgcc gcaacccaac
     8221 aaccagttcc tgcacaaatt gccattgcaa atgccaatac ggtgccctac acccttggag
     8281 cgctggaatc ggcccaaagc gttgccgaac gtttcggtat ttcggtggct gagttacgca
     8341 aactcaacca gtttcgtacg tttgctcgag gttttgataa tgtccgccag ggtgatgaac
     8401 tggatgtccc ggcacaagtt agtgaaaaaa aattaacccc gccgccgggt aataacagtg
     8461 acaacctcga gcaacagata gccagtactt cacagcaaat cgggtctctg ctcgccgaag
     8521 atatgaacag cgagcaagcg gcaaatatgg cgcgtggatg ggcctcttct caggcttcag
     8581 gcgcaatgac agactggtta agccgcttcg gtaccgcaag aatcacgctg ggcgtggatg
     8641 aagattttag cctgaagaac tcccagttcg attttctcca tccgtggtat gaaacgcctg
     8701 ataatctctt tttcagtcag catactctcc atcgtactga cgagcgtacg cagattaaca
     8761 acggcttagg ttggcgtcat ttcactccca catggatgtc gggcatcaac ttctttttcg
     8821 accacgatct tagccgttac cactcccgcg tcggcattgg cgcggagtac tggcgcgact
     8881 atctaaaatt aagcagtaac ggctatttgc gactgaccaa ctggcgcagc gcacctgaac
     8941 tggacaacga ttataaagca cgcccggcca atggctggga tgtacgcgca gaaagctggc
     9001 tacccgcctg gccgcacctt ggcggtaaac tggtctatga acagtattat ggcgattaag
     9061 tggccctgtt cgataaagac gatcggcaaa gtaatcctca tgccataacc gctggactta
     9121 actatacccc cttcccgctg atgaccttca gcgcggagca acgccagggt aaacagggcg
     9181 aaaatgacac ccgttttgcc gtcgatttta cctggcaacc tggcagcgca atgcagaaac
     9241 agcttgaccc gaatgaagtc gctgcacggc gtagccttgc aggcagccgt tatgatctgg
     9301 tggatcgcaa caacaatatc gttctggaat atcgcaaaaa agaactggtt cgcctaaccc
     9361 tgacagaccc cgtgacaggg aagtcaggag aagtgaaatc actggtttcg tcgctacaaa
     9421 ccaaatatgc cctgaaaggc tataacgtcg aagccaccgc actggaagct gccggtggca
     9481 aagtggtcac aacgggtaaa gatattctag ttaccctgcc ggcttaccgg ttcaccagta
     9541 cgccagaaac cgataacacc tggccgattg aagtcaccgc cgaagatgtc aaaggcaatt
     9601 tgtcgaatcg tgaacagagc atggtggtcg ttcaggcacc tacgctaagc cagaaagatt
     9661 cctcggtatc gttaagtacc caaacattga acgcggattc ccattcaacc gccacactga
     9721 cttttattgc gcatgatgca gcaggtaatc ctgttgtcgg gctggtgctc tcgacgcgtc
     9781 acgaaggtgt tcaggacatc accctttctg actggaaaga taatggtgac ggaagctata
     9841 cccagatcct gaccacagga gcgatgtctg gcacgctgac gctgatgcca cagctgaatg
     9901 gtgtggatgc ggctaaagcc cccgccgtgg tgaatatcat ttctgtttcg tcatcccgaa
     9961 ctcactcgtc aattaagatt gataaggacc gttatctctc cggcaatcct atcgaggtga
    10021 cggtagaact gagagatgaa aatgacaaac ctgttaagga acaaaaacag caactgaata
    10081 acgcagtcag catcgacaac gtgaaaccag gagtcactac agactggaaa gaaaccgcag
    10141 atggcgtcta taaggcgacc tataccgcct ataccaaagg cagtggactt actgcgaagc
    10201 tattggaatg aagatttgca taccgctgga tttatcatcg acgccaaccc gcagtcagcg
    10261 aaaattgcga cattatctgc cagcaataat ggtgtactcg ccaatgagaa tgcagcaaac
    10321 accgtctcgg tcaatgtcgc tgatgaagga agcaacccaa tcaatgatca taccgtcacg
    10381 tttgcggtat taagcggatc ggcaacttcc ttcaacaatc aaaacaccgc aaaaacggat
    10441 gttaatggtc tggcgacttt tgatctgaaa agtagtaagc aggaagacaa cacggttgaa
    10501 gtcacccttg aaaatggcgt gaaacaaacg ttaatcgtca gttttgtcgg cgactcgagt
    10561 accgcgcagg ttgatctgca gaagtcgaaa aatgaagtgg ttgctgacgg caatgacagc
    10621 gccacaatga ccgcgaccgt ccgggatgca aaaggcaacc tgctcaatga cgtcatggtc
    10681 actttcaatg ttaattcagc agaggcgaaa ctgagccaaa ccgaagtgaa tagccacgac
    10741 gggatcgcca cagctacgct gaccagtttg aaaaatggtg attatagggt tacggcctct
    10801 gtgagctctg gttcccaggc taatcaacag gtgaatttta tcggtgatca aagtactgct
    10861 gccctgaccc tcagtgtgcc ttcaggtgat atcaccgtca ccaacacagc tccgcaacat
    10921 atgactgcaa ccttgcagga taaaaatggc aacccactaa aagataaaga aatcaccttc
    10981 tctgtgccaa acgacgtcgc aagtaagttc tcgattagca acggaggaaa aggcatgacg
    11041 gatagtaacg gggttgcaat cgcctccctg accggcacgt tagcgggcac gcatatgatc
    11101 atggctcgtc tggctaacag caatgtcagc gatgcacagc caatgacgtt tgtggcggat
    11161 aaagacagag cggttgtcgt tttgcaaaca tcgaaagcgg aaatcattgg gaatggcgtg
    11221 gatgagacaa ctctgacagc aacagtgaaa gatccgtcga atcatccggt ggcggggata
    11281 acggtaaact tcaccatgcc acaggacgtt gcggcaaact ttacccttga aaataacggt
    11341 attgccatca ctcaggccaa tggggaagcg catgtcacgc tgaaaggtaa aaaagcgggc
    11401 acgcatacgg ttaccgcaac gctgggtaat aacaatacca gtgattcgca gccggtaaca
    11461 tttgtggcgg acaaagcctc ggctcaggtt gtcctgcaga tatcaaaaga tgagatcaca
    11521 ggtaatggcg tcgatagcgc aacgctaact gcaacggtta aagatcagtt cgacaatgag
    11581 gtgaataatc ttccggtaac attcagctca gcctcttcag gactcaccct gaccccggga
    11641 gtaagtaata ccaatgagtc tggcatcgcg caggccactc tcgcaggcgt tgcctttggt
    11701 gagcagacgg tcactgcatc actggctaat aatggtgcca gcgacaacaa aactgtgcat
    11761 tttattggcg acacagcggc ggcaaaaatt atcgagttga cgcctgtccc agacagcata
    11821 atcgccggta ccccgcagaa cagctccggc agcgccatca ccgccacagt cgttgataat
    11881 aatggctttc cggtgaaagg tgtgactgtg aacttcacca gcagaacaaa ctctgccgaa
    11941 atgacgaatg gcggccaagc cgtaacgaac gaacagggta aggctaccgt cacttatacc
    12001 aatacccgct cctcgataga atcaggagcg agaccggata ccgttgaggc cagtctggaa
    12061 aatggtagct ccacgcttag cacatcaatt aatgtcaacg ctgatgcgtc tacggcacat
    12121 ctcacctggc tacaggcact ttttgataca gtctccgcag gcgagacaac cagtctgtat
    12181 attgaggtga aggataatta cggcaacggt gtcccccagc atcaggtgac cctcagcgtt
    12241 tcaccaagtg aaggcgtgac ccccagtaat aacgctatat atactaccaa ccacgacggc
    12301 aatttttacg caagctttac cgctacaaaa gccggggttt atcaagtgac ggcaaccctc
    12361 gaaaatggcg attcgatgca acaaacagtg acctatgtgc cgaacgtcac gaatgccgaa
    12421 atcacgctgg caacctcgaa ggatccggtg attgccgaca ataacgatct cacgacacta
    12481 acagcaacag tcgctgatac agagggcaat gcgatagcca acactgaagt aacatttact
    12541 ctgccggaag atgttaaggc gaacttcacg ctgagcgatg gcggtaaagc gattactgat
    12601 gctgaaggca aagcgaaagt cacgctgaaa ggtacaaaag caggcgctca tactgttaca
    12661 gcatcgataa ctggcggtaa gagtgagcag ttggtggtga actttactgc ggatacgctc
    12721 actgcgcagg ttaatcttaa cgttaccgag gacaatttta tcgctaataa cgtcgggatg
    12781 accaggctgc aggcaacagt gactgatgga aacggcaacc cgttagccaa tgaggcggtg
    12841 acattcacgc taccggcaga tgtgagcgca agctttactc tcggacaagg cggttccgcc
    12901 attactgaca tcaacggcaa ggctgaagtt acactgagcg gtacaaaatc cggcacctac
    12961 cccgtgacag ttagcgtgaa caattatggt gtcagtgata cgaaacaggt gactttgatt
    13021 gccgatgctg gtaccgcaac actagcctcc ttaacctctg tatactcatt cgtcgtcagc
    13081 acgaccgaag gcgcgaccat gactgcaagc gtcactgacg ctaacggcaa cccggtagaa
    13141 ggcataaaag ttaatttccg cggaacctcc gtcacgctaa gcagcaccag cgttgaaacg
    13201 gatgatcggg gtttcactga aattcttgtg acaagcaccg aagtcggact gaaaacagtt
    13261 tcagcctctc tggcagataa acctactgaa gtcatatcgc gattactgaa tgcaaaagca
    13321 gatattaatt ctgcaacgat taccagtctg gagatacctg aaggtcaggt catggtcgca
    13381 caagacgtag cagttaaagc tcacgtcaac gaccagtttg gcaacccgat tcttaatgaa
    13441 tctgtaacat tcagtgcaga gccaccagag cacatgacca tcagccaaaa tattgtctct
    13501 actgatacgc atggtatagc cgaggtctcc atgacgcccg aaagaaacgg ttcgtatatg
    13561 gtgaaagcat ccctggcgaa tggagcctca cttgagaaac aactggaggc tattgatgaa
    13621 aaactgacac tctcggcgtc cagcccgctt atcggtgtca attccccaac aggtgcaact
    13681 ctgacggcaa cgctaacttc tgcaaatggc actccagtgg agggtcaggt catcaacttt
    13741 agcgtaacgc cagaaggtgc gacgttaagt ggcggaaaag tgagaactaa ctcttctggt
    13801 caggctccag tcgttctgac cagcaataaa gtcggtacat atacggtgac tgcatcgttc
    13861 cataacggcg taacaataca gacacagaca accgtgaaag tcactggcaa ctcaagcacc
    13921 gcccatgtta ctagctttat cgctgatcca tcgactatcg ccgccaccaa cagtgattta
    13981 agtaccttaa aggcaacggt tgaggatg
//
LOCUS       DAJTYZ010000106        13967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000106 DAJTYZ010000000
VERSION     DAJTYZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.106"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..228)
                     /locus_tag="OO596_003030"
     CDS             complement(<1..228)
                     /locus_tag="OO596_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCR6718826.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="OO596_003031"
     CDS             385..561
                     /locus_tag="OO596_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR6718827.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1187..1486
                     /locus_tag="OO596_003032"
     CDS             1187..1486
                     /locus_tag="OO596_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718828.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OO596_003033"
     CDS             complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OO596_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6718829.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2472..2939)
                     /locus_tag="OO596_003034"
     CDS             complement(2472..2939)
                     /locus_tag="OO596_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6718830.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3104..3973
                     /gene="pldA"
                     /locus_tag="OO596_003035"
     CDS             3104..3973
                     /gene="pldA"
                     /locus_tag="OO596_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6718831.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4106..5935
                     /gene="recQ"
                     /locus_tag="OO596_003036"
     CDS             4106..5935
                     /gene="recQ"
                     /locus_tag="OO596_003036"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6718832.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5999..6616
                     /gene="rhtC"
                     /locus_tag="OO596_003037"
     CDS             5999..6616
                     /gene="rhtC"
                     /locus_tag="OO596_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6718833.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OO596_003038"
     CDS             complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OO596_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6718834.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7409..8431
                     /gene="pldB"
                     /locus_tag="OO596_003039"
     CDS             7409..8431
                     /gene="pldB"
                     /locus_tag="OO596_003039"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6718835.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8439..9239
                     /gene="yigL"
                     /locus_tag="OO596_003040"
     CDS             8439..9239
                     /gene="yigL"
                     /locus_tag="OO596_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6718836.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9315..10214
                     /gene="bioP"
                     /locus_tag="OO596_003041"
     CDS             9315..10214
                     /gene="bioP"
                     /locus_tag="OO596_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6718837.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OO596_003042"
     CDS             complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OO596_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6718838.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11291..13552
                     /gene="metE"
                     /locus_tag="OO596_003043"
     CDS             11291..13552
                     /gene="metE"
                     /locus_tag="OO596_003043"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6718839.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
BASE COUNT         3398 a         3388 c         3654 g         3527 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgat
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtat ttcctgtaac
     1441 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
     1501 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
     1561 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
     1621 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
     1681 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
     1741 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
     1801 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
     1861 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
     1921 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
     1981 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     2041 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     2101 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     2161 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     2221 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     2281 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     2341 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     2401 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     2461 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     2521 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     2581 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     2641 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     2701 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     2761 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     2821 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     2881 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     2941 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     3001 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     3061 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     3121 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     3181 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     3241 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     3301 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     3361 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     3421 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     3481 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     3541 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     3601 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     3661 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     3721 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     3781 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
     3841 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     3901 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
     3961 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     4021 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     4081 tttatttcag gcaatcgggg ggaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     4141 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     4201 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     4261 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     4321 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     4381 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     4441 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     4501 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     4561 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     4621 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     4681 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     4741 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     4801 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     4861 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     4921 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     4981 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     5041 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     5101 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     5161 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     5221 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     5281 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     5341 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     5401 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     5461 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     5521 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     5581 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     5641 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     5701 tcgtaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     5761 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     5821 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     5881 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     5941 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     6001 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     6061 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     6121 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     6181 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     6241 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     6301 tgcgccacag gtcgagctgg cgaaaagtgg acgcagtttc ctgaaaggtt tactgaccaa
     6361 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     6421 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     6481 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     6541 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     6601 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     6661 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     6721 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     6781 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     6841 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     6901 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     6961 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     7021 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     7081 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     7141 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     7201 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     7261 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     7321 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     7381 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     7441 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     7501 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     7561 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     7621 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     7681 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     7741 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     7801 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     7861 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     7921 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     7981 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     8041 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     8101 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     8161 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     8221 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     8281 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     8341 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     8401 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     8461 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     8521 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     8581 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     8641 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     8701 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     8761 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     8821 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     8881 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     8941 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     9001 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     9061 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     9121 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     9181 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     9241 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     9301 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     9361 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     9421 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     9481 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     9541 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     9601 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     9661 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gttaccgacc
     9721 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     9781 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     9841 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     9901 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     9961 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
    10021 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
    10081 agccgcactg gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc
    10141 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
    10201 cgctgagcga ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc
    10261 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cttggcgctc
    10321 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
    10381 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
    10441 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct
    10501 ggcgagatct tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac
    10561 cagacgcact tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
    10621 ggaggtcatc accagatcca gctcgccctg ttgcaacgca ggctgcgggt caaacgtcac
    10681 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
    10741 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
    10801 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
    10861 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
    10921 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
    10981 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
    11041 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
    11101 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta
    11161 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
    11221 ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
    11281 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
    11341 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
    11401 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
    11461 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
    11521 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
    11581 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    11641 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
    11701 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
    11761 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    11821 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    11881 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    11941 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    12001 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    12061 gcctgttcag ggtctgcatg ttgatctcgt acatggtaaa gatgacgttg ctgaactgca
    12121 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    12181 gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    12241 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    12301 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactagc
    12361 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    12421 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
    12481 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
    12541 gcgtgcacgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
    12601 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
    12661 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
    12721 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    12781 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    12841 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
    12901 gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg gcccggtgac
    12961 tattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    13021 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
    13081 gattgacgaa ccggctctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
    13141 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    13201 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcact
    13261 ggacgcagac gtcatcacca tcgaaacctc gcgttctgac atggagttgc tggagtcgtt
    13321 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
    13381 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    13441 agagcgcctg tgggttaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    13501 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt
    13561 aacgagtcac tgtaaattgc agtacgatga aagaggcaaa actgcctgat actaggccta
    13621 cataatttat gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc
    13681 gcatccggca tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc
    13741 cattttgcta tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata
    13801 taatcctgag cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa
    13861 attgtatccc ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat
    13921 aataaccaaa ccctcgttag ctaatttgtt aacaatgtta acaacat
//
LOCUS       DAJTYZ010000107        13893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000107 DAJTYZ010000000
VERSION     DAJTYZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.107"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            232..1485
                     /gene="kch"
                     /locus_tag="OO596_003044"
     CDS             232..1485
                     /gene="kch"
                     /locus_tag="OO596_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6718840.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1540..1713)
                     /locus_tag="OO596_003045"
     CDS             complement(1540..1713)
                     /locus_tag="OO596_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6718841.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1856..3316
                     /gene="cls"
                     /locus_tag="OO596_003046"
     CDS             1856..3316
                     /gene="cls"
                     /locus_tag="OO596_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6718842.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3351..3680
                     /locus_tag="OO596_003047"
     CDS             3351..3680
                     /locus_tag="OO596_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6718843.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3733..4737)
                     /gene="oppF"
                     /locus_tag="OO596_003048"
     CDS             complement(3733..4737)
                     /gene="oppF"
                     /locus_tag="OO596_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6718844.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4734..5747)
                     /gene="oppD"
                     /locus_tag="OO596_003049"
     CDS             complement(4734..5747)
                     /gene="oppD"
                     /locus_tag="OO596_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6718845.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5759..6667)
                     /gene="oppC"
                     /locus_tag="OO596_003050"
     CDS             complement(5759..6667)
                     /gene="oppC"
                     /locus_tag="OO596_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6718846.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6682..7602)
                     /gene="oppB"
                     /locus_tag="OO596_003051"
     CDS             complement(6682..7602)
                     /gene="oppB"
                     /locus_tag="OO596_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6718847.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7688..9319)
                     /gene="oppA"
                     /locus_tag="OO596_003052"
     CDS             complement(7688..9319)
                     /gene="oppA"
                     /locus_tag="OO596_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6718848.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9603..9899
                     /locus_tag="OO596_003053"
     CDS             9603..9899
                     /locus_tag="OO596_003053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718849.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10057..10704)
                     /gene="ychE"
                     /locus_tag="OO596_003054"
     CDS             complement(10057..10704)
                     /gene="ychE"
                     /locus_tag="OO596_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6718850.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11182..13857
                     /gene="adhE"
                     /locus_tag="OO596_003055"
                     /gene_synonym="adhC"
     CDS             11182..13857
                     /gene="adhE"
                     /locus_tag="OO596_003055"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6718851.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3656 a         3434 c         3228 g         3575 t
ORIGIN      
        1 gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt tatatgatgt
       61 gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa atcctttgat
      121 tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat gtatgttcag
      181 aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc agtgagtcac
      241 tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca cgacattctc
      301 gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg tatgtcggtc
      361 aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc cttgctggca
      421 aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg cttactgttc
      481 cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct aatttacacc
      541 ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac gctggtgttt
      601 ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac aatttttgca
      661 tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta tttaagcgaa
      721 ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat agaaaccatg
      781 tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt gttcactatt
      841 tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat ttttggtccg
      901 cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat gcatcgtaaa
      961 gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct gcaactgaat
     1021 caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat caagcaactt
     1081 gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga cagttcagta
     1141 ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga taacgatgca
     1201 gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa aacagttctc
     1261 gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga tatcattctc
     1321 tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga agagattaat
     1381 aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag cgataacgat
     1441 gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt aaagacgaaa
     1501 gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt agatcgcaaa
     1561 caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga tacgcatatt
     1621 tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac gctgcatgcg
     1681 ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca ataacagaat
     1741 ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt tatttgccag
     1801 atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct aatctatgac
     1861 aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc tcattgcagg
     1921 cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg cctggctgtt
     1981 gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg gcgagctcca
     2041 tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg caaaatggct
     2101 taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg tcgctgcgcc
     2161 attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga atcagctaca
     2221 actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc agctcgcgcg
     2281 ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg accaggtggc
     2341 tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc tcgactccgc
     2401 cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg ccggtattga
     2461 agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta tggacctgcg
     2521 ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca gcatgaatat
     2581 ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg atctgatggc
     2641 gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg actgggagat
     2701 tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc cgtttgaaca
     2761 ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg aagatctcat
     2821 tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca tgacaacgcc
     2881 ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc agcgcggggt
     2941 ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct gggccagtcg
     3001 cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg aaggcgggtt
     3061 actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca ccgttaacct
     3121 tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg ataaaggttt
     3181 tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc tgctcgatgc
     3241 ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt tttacttctt
     3301 cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact atggatatgg
     3361 atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat atttttctcg
     3421 aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt cagtttgaag
     3481 agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat gttgatttcg
     3541 atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt gaggatggtg
     3601 aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac aaactttgcc
     3661 atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga caactgtcag
     3721 cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc tgaaactccc
     3781 ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac aggtacggaa
     3841 aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta actggatggt
     3901 tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg ccctggtgta
     3961 aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg catggccgag
     4021 atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat gagcgataaa
     4081 aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca cctgcgcctg
     4141 aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg gttcaagaat
     4201 aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat gcggatagcg
     4261 gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc gctcgcgaac
     4321 ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga tctcgccgat
     4381 ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa tatcactgcg
     4441 aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc aggcaacatg
     4501 accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact taccgcatcc
     4561 cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga caccatcgac
     4621 ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga tttcaaagtg
     4681 cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag cattcataac
     4741 agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc ttccagcggc
     4801 ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg gcaacctttc
     4861 ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc accttccgca
     4921 tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg ttgataaaag
     4981 acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag cactttgtcg
     5041 cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt attaaattcc
     5101 cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc cagcgcagta
     5161 gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc aatcataact
     5221 cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt acgtgcttcc
     5281 ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc tttgctcatg
     5341 ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata gggattcaac
     5401 gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt attgagttca
     5461 cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc aatacgtccg
     5521 ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga accagactca
     5581 cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt gaccgccgtg
     5641 acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag cagtacgtca
     5701 gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc tgcactcctt
     5761 aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg aaacaaaaca
     5821 gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact tccatcgagt
     5881 tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta cccaacccca
     5941 ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat gcgtagacca
     6001 ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat gtcgatacac
     6061 cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt tgtccacgca
     6121 caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc agcaggatgt
     6181 tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag gagttgagga
     6241 tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg gaaagcgaac
     6301 cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc atgagtgaga
     6361 tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca gtaccaaagt
     6421 agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta tcgtcatagg
     6481 caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc agcactatca
     6541 gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc cacaagctgc
     6601 gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt ttcttactta
     6661 acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg catatagcac
     6721 atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca ggcttaacac
     6781 taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc ccggcaaacc
     6841 ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg ggcccatata
     6901 agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc gccgcatagg
     6961 taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa tcatagagcc
     7021 tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca tcggcaatat
     7081 catgaattta agcgccccac cattccagcc accgcccggc agccaatgca aaatgatcgc
     7141 aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc cggtcattgc
     7201 cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa taacgccagc
     7261 actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg cagaaacggg
     7321 aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg atggaccgaa
     7381 atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg gatcattaag
     7441 atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt cgccggtaaa
     7501 agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa taaatagcgt
     7561 cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac cttctggcct
     7621 gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc tgccccacgt
     7681 attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc agcggatctt
     7741 tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag taataaacag
     7801 gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga gctgtgcgct
     7861 gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc gggctcttat
     7921 aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa gttggttcgt
     7981 tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga cgggtgtcga
     8041 ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt ttcttccaca
     8101 atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac agcaggttga
     8161 tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc gcttcttcgt
     8221 tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg ccatcagtat
     8281 acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta ttaacaatga
     8341 tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg aatggcggtt
     8401 tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga acttcgtccg
     8461 ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac gtcatgtcga
     8521 tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc aaataggtta
     8581 cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca agaacgattc
     8641 gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata ttaccaggct
     8701 gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa gttgatgggt
     8761 gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct aatgtgtgat
     8821 catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga atttcatcaa
     8881 taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg ttcggatcaa
     8941 cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta tcagaccatt
     9001 tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc caggattcag
     9061 cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca aacaggtctc
     9121 ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac tgaacttctg
     9181 aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg ggtacatcag
     9241 ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct actaaacttc
     9301 tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg gtattaacca
     9361 gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc tgtcaggtta
     9421 ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca gacgatcacc
     9481 ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg cgacgctctt
     9541 taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc ggaaagtacc
     9601 aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc tcttttattg
     9661 accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca attcaatcaa
     9721 atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt gaaatgcaca
     9781 ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt ggaattacta
     9841 atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa ataacatgat
     9901 ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga agaatatttt
     9961 tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa tgacgaagta
    10021 aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc cggggaaaat
    10081 ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata gccccataat
    10141 acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg gtgccatgcg
    10201 gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac caaacagata
    10261 gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc ctggccccgc
    10321 catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg tttctgattt
    10381 ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca tcgctatcgt
    10441 caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa aaagttgcag
    10501 aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca ggttggctgt
    10561 aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga taaagacggg
    10621 aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga agtaaacggg
    10681 aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat aatgttagcc
    10741 ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt tttcacctcc
    10801 taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa catgctaatg
    10861 tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa aagcgatgct
    10921 gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc cagaagtgag
    10981 taatcttgct tacgccacct agaagtgacg cattagagat aataactcta atgtttaaac
    11041 tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc ataggttaag
    11101 caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac taaaaaagtt
    11161 taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa cgcactcgta
    11221 gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca agtagacaaa
    11281 atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc gaaaatggcc
    11341 gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca ctttgcttct
    11401 gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc tgaagacgac
    11461 acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat cgttccgacc
    11521 actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac ccgtaacgcc
    11581 attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc tgatatcgtt
    11641 ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat cgatcaacct
    11701 tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat cctcgcgact
    11761 ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat cggtgtaggc
    11821 gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc agttgcatct
    11881 gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca gtctgttgtt
    11941 gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg cggctatctg
    12001 ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg tgcgctgaac
    12061 gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt ctctgtacca
    12121 gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga accgttcgca
    12181 catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga agacgcggta
    12241 gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg cctgtacact
    12301 gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa aacggctcgt
    12361 atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa cttcaaactc
    12421 gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc tgaaaacgtt
    12481 ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa catgttgtgg
    12541 cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc gctggatgaa
    12601 gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct gttcaacaat
    12661 ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac tgaagtcttc
    12721 ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga actggcaaac
    12781 tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga cgccgcgaag
    12841 attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc gctgcgcttt
    12901 atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc gaaaatgatc
    12961 gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt tgtaactgac
    13021 gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga tatggcgatt
    13081 gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg tggtctggac
    13141 gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt ctctgatggt
    13201 caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta ccacgaaggg
    13261 tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc gggtatcgca
    13321 tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg ttcccagttc
    13381 catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg ctacaatgcg
    13441 aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca ggctcgccgt
    13501 cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg tactgctgct
    13561 aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg tattccgaaa
    13621 tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga taaactgtct
    13681 gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct gatctccgag
    13741 ctgaaacaga tcctgctgga tacctactac ggtcgtgatt atgtagaagg tgaaactgca
    13801 gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc cgcttaatca
    13861 gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAJTYZ010000108        13826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000108 DAJTYZ010000000
VERSION     DAJTYZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.108"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(20..778)
                     /gene="dsbG"
                     /locus_tag="OO596_003056"
     CDS             complement(20..778)
                     /gene="dsbG"
                     /locus_tag="OO596_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6718852.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTPTY"
     gene            1150..1713
                     /gene="ahpC"
                     /locus_tag="OO596_003057"
     CDS             1150..1713
                     /gene="ahpC"
                     /locus_tag="OO596_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6718853.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1884..3449
                     /gene="ahpF"
                     /locus_tag="OO596_003058"
     CDS             1884..3449
                     /gene="ahpF"
                     /locus_tag="OO596_003058"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6718854.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3570..3998)
                     /gene="uspG"
                     /locus_tag="OO596_003059"
     CDS             complement(3570..3998)
                     /gene="uspG"
                     /locus_tag="OO596_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6718855.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4219..5457
                     /locus_tag="OO596_003060"
     CDS             4219..5457
                     /locus_tag="OO596_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6718856.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5688..6098)
                     /gene="rnk"
                     /locus_tag="OO596_003061"
     CDS             complement(5688..6098)
                     /gene="rnk"
                     /locus_tag="OO596_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6718857.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6328..7134)
                     /gene="rna"
                     /locus_tag="OO596_003062"
     CDS             complement(6328..7134)
                     /gene="rna"
                     /locus_tag="OO596_003062"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6718858.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7248..8711)
                     /gene="citT"
                     /locus_tag="OO596_003063"
     CDS             complement(7248..8711)
                     /gene="citT"
                     /locus_tag="OO596_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR6718859.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8762..9640)
                     /gene="citG"
                     /locus_tag="OO596_003064"
     CDS             complement(8762..9640)
                     /gene="citG"
                     /locus_tag="OO596_003064"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6718860.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9615..10166)
                     /gene="citX"
                     /locus_tag="OO596_003065"
     CDS             complement(9615..10166)
                     /gene="citX"
                     /locus_tag="OO596_003065"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR6718861.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10170..11702)
                     /gene="citF"
                     /locus_tag="OO596_003066"
     CDS             complement(10170..11702)
                     /gene="citF"
                     /locus_tag="OO596_003066"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6718862.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11713..12621)
                     /gene="citE"
                     /locus_tag="OO596_003067"
     CDS             complement(11713..12621)
                     /gene="citE"
                     /locus_tag="OO596_003067"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6718863.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12618..12914)
                     /gene="citD"
                     /locus_tag="OO596_003068"
     CDS             complement(12618..12914)
                     /gene="citD"
                     /locus_tag="OO596_003068"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6718864.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12929..>13826)
                     /gene="citC"
                     /locus_tag="OO596_003069"
     CDS             complement(12929..>13826)
                     /gene="citC"
                     /locus_tag="OO596_003069"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6718865.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3399 a         3502 c         3675 g         3250 t
ORIGIN      
        1 atctgaacat aaaacactat caataagttg gagtcattac ccccataatg atattaagcg
       61 ttttctgatc gggcaacccc acggcctgtt gcagcgtatt ttccttactc atgtaataga
      121 tagccggcgt gacatttgcc cccagatcgt ccatcagttt ctcattgtca cttaacactt
      181 tcatttgctc tgtacttacg tttgcaggca cgtttagctt aagcttgcca ccagaggctt
      241 catattgttg ccaggttttt gcgggatctt tggaggcaag aattgccgct gctgtcgccg
      301 ggctttctgg cttgataacc ccaaccaaca atgttcttaa ttgcactttg ccagaatcta
      361 cccacgggcg cgcctgctgc cagaactgtt tacaatatgg gcagaacgga tcggcgaaga
      421 cgtagacaat gaccggcgca tcttttttac cgtcgaggat ccagtgggat tgttccatcc
      481 gttgccacat ttcgcgtccg gctggtgcgt aaatttcttt ttcgataagt gtgttactca
      541 ggttttcacc tttctcgttg tacatgtaac cagagatagc gtgcttacca tcgggagtca
      601 ggtagatggt gacgcccata tcctgatact ttccgagata acctttcatt cctccggggg
      661 catcgaacgt tttgatgatt gtaatgccct gtttttcaat cgcttttact ggggaaggaa
      721 gttcctctgc gaaggcgatt gcaggaagca gagccagtaa aagtatcttt tttaacatta
      781 atttgtcctt ttcagtcagt gcaaaagtcg agtaaaaggc ataacctatc actgtcatag
      841 gtaagagctt agatcaggtg attgcccttt gtttatgagg gtgttgtaat ccatgtcgtt
      901 gttgcatttg taagggcaac acctcagcct gcaggcaggc gctgaagata ccaaagggta
      961 gttcagatta cacggtcacc tggaaagggg gccattttac tttttatcgc cgctggcggt
     1021 gcaaagttca caaagttgtc ttacgaaggt tgtaaggtaa aacttatcga tttgataatg
     1081 gaaacgcatt agccgaatcg gcaaaaattg gttaccttac atctcatcga aaacacggag
     1141 gaagtataga tgtccttgat taacaccaaa attaaacctt ttaaaaacca ggcattcaaa
     1201 aacggcgaat tcatcgaaat caccgaaaaa gataccgaag gccgctggag cgtcttcttc
     1261 ttctacccgg ctgactttac tttcgtatgc ccgaccgaac tgggtgacgt tgctgaccac
     1321 tacgaagaac tgcagaaact gggcgtagac gtatacgcag tatctaccga tactcacttc
     1381 acccacaaag catggcacag cagctctgaa accatcgcta aaatcaaata tgcgatgatc
     1441 ggcgacccga ctggcgccct gacccgtaac ttcgacaaca tgcgtgaaga tgaaggtctg
     1501 gctgaccgtg cgaccttcgt tgttgacccg cagggtatca tccaggcaat cgaagttacc
     1561 gctgaaggca ttggccgtga cgcgtctgat ctgctgcgta aaatcaaagc agctcagtac
     1621 gtagcttctc acccaggtga agtttgcccg gctaaatgga aagaaggtga agcaactctg
     1681 gctccgtctc tggacctggt tggtaaaatc taaatttcct tcgtctttcg cgctaccagc
     1741 tccggcgcga aatacttcgg aaattcacct aattcttcgg gtgctgcggc acccgatttt
     1801 cttccccgca ccatgatgca agctgcatcc aggtagccgt agaggccgct tgcatgatga
     1861 tgtttaaagc ccaggagata aacatgctcg acacaaatat gaaaactcaa ctcaaggctt
     1921 accttgagaa attgaccaag cctgttgagt taattgccac gctggatgac agcgctaaat
     1981 cggcagaaat caaggaactg ttggctgaaa tcgcagaact gtcagacaaa gtcaccttta
     2041 aagaagataa cagcttgccg gtgcgtaagc cgtctttcct gatcaccaac ccaggttcca
     2101 accaggggcc acgttttgca ggctccccgc tgggccacga gttcacctcg ctggtactgg
     2161 cgttgctgtg gaccggtggt catccgtcga aagaagcgca gtctctgctg gagcagattc
     2221 gccatattga cggtgatttt gaattcgaaa cctattactc gctctcttgc cataactgcc
     2281 cggacgtggt gcaggcgctg aacctgatga gcgtactgaa cccgcgcatc aagcacactg
     2341 caattgacgg cggcaccttc cagaacgaaa tcaccgatcg caacgtgatg ggcgttccgg
     2401 cagtgttcgt aaacgggaaa gagtttggtc agggccgcat gacgttgact gaaatcgttg
     2461 ccaaaattga tacgggcgcg gaaaaacgtg cggcagaaga gctgaacaag cgtgatgctt
     2521 atgacgtatt aatcgtcggt tccggcccgg cgggtgcagc ggcagcaatt tactccgcac
     2581 gtaaaggcat ccgtaccggt ctgatgggcg aacgttttgg tggtcagatc ctcgataccg
     2641 ttgatatcga aaactacatt tctgtaccga agactgaagg gcagaagctg gcaggcgcac
     2701 tgaaagttca cgttgatgaa tacgacgttg atgtgatcga cagccagagc gccagcaaac
     2761 tgatcccagc agcagttgaa ggtggtctgc atcagattga aacagcttct ggcgcggtac
     2821 tgaaagcacg cagcattatc gtggcgaccg gtgcaaaatg gcgcaacatg aacgttccgg
     2881 gcgaagatca gtatcgcacc aaaggcgtga cctactgccc gcactgcgac ggcccgctgt
     2941 ttaaaggtaa acgcgtagcg gttatcggcg gcggtaactc cggcgtggaa gcggcaattg
     3001 acctggcggg tatcgttgag cacgtaacgc tgctggaatt tgcgccagaa atgaaagccg
     3061 accaggttct gcaggacaaa ctgcgcagcc tgaaaaacgt cgacattatt ctgaatgcgc
     3121 aaaccacgga agtgaaaggc gacggcagca aagtcgttgg tctggaatat cgagatcgtg
     3181 tcagcggcga tattcacaac atcgaactgg ccggtatttt cgtgcagatt ggtctgctgc
     3241 cgaacaccaa ctggctcgaa ggcgcagtcg aacgtaaccg catgggtgag attatcattg
     3301 atgcgaaatg cgaaaccaac gtcaaaggcg tattcgcagc gggtgactgt acgacggttc
     3361 cgtacaagca gatcatcatc gccactggcg aaggtgccaa agcctctttg agtgcttttg
     3421 actacctgat tcgcaccaaa actgcataag aagaagtaag attcacctgc aattgcttag
     3481 ccgccggggt caaacctggc ggctttttta tggcattaaa aagccctgcc gggatgttcc
     3541 aggcagggcg gcataaatac ttttatctgt tagcgaatga ccagtacagg cagattggcg
     3601 tggcggatca cgctcgaggc gttagaacct aacagatggg tcgaaatcga tgggttgcga
     3661 gaaccgataa ctacaacatc agcccccagt tcttctgcca actcattgac ttcatcccgc
     3721 acgctaccaa aacggacatg ttgtttaatg cgggaaggat cgatggtgaa gtggctgacc
     3781 atcgtttgca gacgttcttc tgcttcatgt tgcagatgct cttcaaaacg acgcacatca
     3841 gcggcaaaac ggtgcaggct caggctggct gacccgggta gtacgtgaag tagatgaata
     3901 actccgtcat cctgggcgag gaattcagcg tggcgaacag ctttgtcgct caattccatt
     3961 tcaaatacat caactggcat aatgattgtc ttatacataa ccctttctcc ctgttaatca
     4021 tgaacaaatc attcgccatg attataatat ttatccctga tatttgtctg gttctttttc
     4081 cttacgaacg gtttctgtga ttaatatatt ctcactgaac accaggaatt ctcccaaaac
     4141 ctgcggtacc gcccgttttc ccgctgtgat agctaccctt aaagactgac tcttttttga
     4201 actgtctctg gaggttgcat gaaagcattg acttatcacg gcccacatca cgttcaggta
     4261 gaaaatgttc ccgatccggg cattgaacag gctgatgata ttattctgcg tattacggca
     4321 acggcgatct gtggctctga cctccatctt tatcgaggca aaatacccca ggttaaacat
     4381 ggcgatattt tgggtcatga atttatgggg gaagtcgttg aaaccggaaa ggacgtaaaa
     4441 aatttgcaaa aaggcgaccg ggtggtaatt ccgtttgtca ttgcttgtgg cgactgtttt
     4501 ttctgtcgaa tgcagcaata tgccgcctgc gaaaatacca atgcgggtaa aggcgctgcg
     4561 ctcaataaaa aacagatacc agctcccgcg gcattgtttg gttatagtca cctgtatggt
     4621 ggcgttcctg gtggacaggc ggaatatgtc cgcgtcccta aagggaatgt ggggccgttt
     4681 aaagtaccgc ctttgctttc agatgataaa gcccttttcc tttctgatat tctgccaacg
     4741 gcatggcagg cagcaaaaaa tgcgcagatc caacaaggtt caagcgttgc agtctatggt
     4801 gccggtccag tgggattgtt gacaatcgcc tgtgcacggt tgctcggtgc ggaacagatt
     4861 tttgttgttg atcatcatcc ctaccgcttg cgtttcgccg ctgaccgcta cggcgcgatc
     4921 ccgattaatt tcgatgaaga tagcgatccg gcacagtcaa ttattgaaca aacggcaggt
     4981 caccggggcg tggatgcagt aatagacgcc gtcggttttg aagcgaaagg cagcaccacg
     5041 gaaacggtgc tgactaacct gaaactggag ggcagcagcg gtaaagcgtt gcgccagtgt
     5101 attgcggcgg tcaggcgtgg cggcattgtt agcgtaccgg gcgtctacgc tggatttatt
     5161 cacggtttcc tgtttggcga cgcctttgat aaagggttga cgtttaaaat gggacagacc
     5221 cacgttcacg catggctggg tgaattatta ccgttaattg agaaaggatt actgaaacca
     5281 gaagaaattg ttacccacta tatgccgttt gaagaggccg cccggggata tgagattttc
     5341 gaaaaacgtg aagaggagtg ccgtaaggtg attctggtac ccggtgcaca aagcgcagag
     5401 gcggcgcaga aggcggtttc aggtctggtg aatgcgatgc cggggggaac aatatgatcg
     5461 tcaggagtgg ttttcgaggt aaaggacagc catgacgata atcgccgcca taatcagaaa
     5521 tcctatcagg atgtaaaatg cttctgccat ggttattccc acaaacgaaa cggaataatt
     5581 ttgcagcaaa agtgaacagt gagaaccagg aaaaattgct gattttgcgt aaagaggatg
     5641 cgagtgcatc ctctgggcaa agcgagttat cgcttgtgca gatgggatta aagcaggtag
     5701 tcgccagcag cttctggctg gtattcgagt tccagcactt caaggtgggt tgcaacgccg
     5761 cccggaagtt cccagtgaat ggaatcgcca acgcgcagcc ccagcagtgc ggcacctacc
     5821 ggagccataa cggaaagctg agtattgcta tcggtcattt ttgccggata caccagcgtg
     5881 cgcacacgca cttcgccatc gctaagattg cggaatttaa cccggctgtt cattgtcacc
     5941 acgtcgtgtg gcatctcttc tggcgaacac atttgggcgc gatccaactc tgcgtttaac
     6001 gcgtcggcga ttggcaaacc agcataggcg ggttgctcca gcagaatatc gatgcgttcg
     6061 gcatccaggt cgttaatgat gatagttggt ctggacattt ttactccatg tcgtcggtac
     6121 tgcgagtgtc gcagataaac atacccaaaa gaaaaccctc accgtcaggc ggcgagggtt
     6181 taactcacat gatgatactg actgttgctc actctttgaa gtgatttgcg tcacattcag
     6241 ggaattcctc aatgcagcgc attatgtata attcttaatc gtcacggttt atggaagacg
     6301 aattagcgtg ttttgtaaat cagatgatta ataacccact ttatcaatca caaagttttt
     6361 gccacagttg cctgggtgtg gttgtggcaa gaatgagttt gcagaaagcg gagcgttgat
     6421 ggcgtcagct ttgatcgaga tctgaatttc agtcaaatac gcagggttac cctggcacgt
     6481 tagcttaact gctttcacgt tctcttttcc ccagcttttg gcaaaggcgg cgtcaaagtc
     6541 acggcggctc actgttttac cgtaattatc cgcaaggaat tttccggctt cgctctcttt
     6601 aatttcttga ttcaggcgta ccatcgtacc gaagtatgcg tccggatcaa aaccaaagca
     6661 ggcaccgtgt ttggcatatt cgtagcgttc caggcaggaa cgtccgccag ctcctggcat
     6721 gacttcactt agtttagcgg ccgtttccag tgataacccg gtttccggcg atgaacacat
     6781 ccggctggcg tgcgcttctg gtagattcgg gattgggcga gtagcgcaac cgaagcgcat
     6841 ccagcggcgt tcatcaacac cacgggcagc aaccgattta ggcaatcctg gccacagacc
     6901 atgtacggtc agaaaatcag ctttgttggt cgtttcggtt tgtaggcgac attcatctcg
     6961 ttcgttacga tttcgatcgt gttgactctg gcaaaatccg gtttgccagg agagggccag
     7021 gacatagcga tcaaaatcgc catactgttt tgcctgcaac gctacggcgt tggcagaaga
     7081 gaagggaagc agagaaaccg cgagcaacgc ggcgttacgc cagaatgctt tcataatggg
     7141 tgtggaactc atacatacac tgaatactat ctattaaatc ataaaaaagc ccgccaggtg
     7201 ggcttacctg gcgggcgtga tgatttattc agcgtttggc gaacgtatta gttccacatg
     7261 gcgagaatcg gccagccaac caacagcaac atagagatgt aaatcacccc gaagattgcg
     7321 ccaagacgcc agtaatcttt tgatttcaca tagccacagc cgtaaataat caccccagga
     7381 ccggttgcat acggcgtcag acagcccatg ataccgatag acagcaccag caggatacac
     7441 agttgttcca ttggtacgcc cggaatacct ttaccgacgg ccagaataac cgacagcatg
     7501 gttgcggtgt gcgcagacag gctggcaaac aggtagtgtg caaagtagaa caccagaacc
     7561 agtacaatca ccgttgcgtt tggtgagaat ccttccaggt gcgtactcat ggtaccggcg
     7621 aaccagtcaa taaagccaga acgagtcagg ccgttagcca tcacaaccag agttgccagg
     7681 ttgaccagcg tgttccatgc gctgttatag cgggtaatat ctttccaagg cacaacgtgc
     7741 agggccagca ttagcgaaac tgccagcaga ccaactgcag tagcattaat gacttcactg
     7801 ccaaaaaccc acaaacctaa gctgagcaat acaaggccaa tcagtgtcca ctctctgcgt
     7861 gtcagcgcac ccatggtttt cagttcatca cccgcccagg ttgccacttc ttcactgtgt
     7921 gtgatttccg gtttgtacag cacgtaggaa agccacggcg caatgataag caagataacc
     7981 ccaaccggca ggaagcagag gaaccactgc aaccagctaa tctggatacc ggcaattttg
     8041 ctgacgaact ccagacccag cacgtttggt gccgcaccgg tgacaaacat ggacgaactc
     8101 agactggtac taatgaccat catccacatc aaatagccgc caatacgacg cgcggacgga
     8161 tcgttgggga atgatttaaa cagcggcggc aggtttttaa tgaccggaaa aaccgtaccc
     8221 ccggtacgcg cggtgttgga cggtgtaaac ggtgccagca gaatgtcgat aatgacaatc
     8281 gcataaccca acgtcagcgt gcgtttgccc atgaatttca ccaggaaaag ggcaatgcga
     8341 cgacctaacc cggaaacttc ataccctaat gcaaaaataa atgcgccaaa taccagccat
     8401 accgtggtgc tggaaaaacc agccagaccc catttcagcg cctgtttttg cgcattaaac
     8461 gctgggtcag ctaattcttt ggcatcaaag agcaggtaat tactgccaat aacgcaaata
     8521 gtaaccgcaa taaaactgat cgctgttgcc ggaattggct cgaggatcat gccgacaatc
     8581 attgccacaa acacagcgaa gtaatgccat gcctgtggtg gcataccgtc ggggacaggg
     8641 ataagaaaca tgacacccat caccaccagt ggggccaata gtttccatat attatctttt
     8701 gctaaagaca tacgggttct ccgaaaatta atatttccaa atttatcaag tgcttaaata
     8761 attaaatctg tgctaaaaac caggtaagga tcagcaggtc ggcactgccg cctggactga
     8821 gatttcgttc gatacactcc ctgtcgaact gccggagata atcgagatcg gcgggggttc
     8881 gaatgccccc tttttgcaat aatgtttgcg cctcgcgctg tagccagcgc aggcccccct
     8941 cgccaccgcg cgatgcaacg ttggtatcgc cgttgatcgc catcagtagg agcaaggtat
     9001 cgagcaatgc cagttcagga tctaacccct gatccagcag agtgaggtaa tgcggcaagg
     9061 cgtgattgat caccagtgga taacccgctt cggcttcacc gcgtgcgccg gtaaggccaa
     9121 gctgctggta caaccgttga cctgccgtca gttgtgaatt attggtacgc agttctcgat
     9181 cggtcaggcc acggcagaaa cttgccgccg tagaacaaac ggttgttggc gttaccggtt
     9241 ggttgagttg aagcaaacgg ccaattgccg cacatagtag ccctaaagaa aaaatgctgc
     9301 ctttatgcgt gtttacgccc gcagtggcgc ggaacatatc accttcgcaa gccataccaa
     9361 ttgggcgtaa tccgtggagt accgcttctg gtgctatttc cgcactacag gcaccaaatt
     9421 caatgaaacg gggtagccag ccctgaatcg ccagcgcgct gcggtggaaa tcttccagcg
     9481 ccatatcttt atgtgcaccg cagttaatcc gatccacgag gcctggtttc ggtgacagat
     9541 tgacttcagt cagcatggcg cgccagccca gcagggcgta ctcatcgatc aatgacgtcg
     9601 caagctttgt ggttttagtt gacgttgcag gcatcgacat cgttcagcag tgcctccatg
     9661 cggttgagta aatcggtcag ttgatgggtt tttccacgcg cgcagacggc tgcgctttgc
     9721 tcgcacaaca ggcagcggcg aggcggcagt gaatagtcgc ggcgggagag aatttcgcct
     9781 tcgggcgtca ggacatcgat atcccataac cgcccgagag gatgactatg ttcaagctca
     9841 atggtggcga gcttgaggtc gcgagccggg gcggcaatgc tcaacatgcc ctccggcccg
     9901 ctggcggaaa ccagtgcagc ctgctcctga atttgccagc cctgttttgc ggctaaggca
     9961 cgcaaggctg tcacgccatg attaaaaatt cggcgtgtga cctcgctgtc tttaatcggc
    10021 ccaggcgcaa ccacggtaaa ggagaccagt ggaacaggat ggcgcttgag ccagacgtgt
    10081 tgccgtgctt gcctttcatc ccggctgacg agcagctcgg gaattgacac cgcatggtgg
    10141 ctggcgagtt caggaagcag gtgcatggct tattccttca cctgatgcac aacatcgatc
    10201 accgagccat cgcggtaacg cacaacggca acgacgcggt cggtgaattc aatcggctgt
    10261 ggttcaccgg tcagcagacg cgcgcgttcg cgcagccact caatggaaac cactttaatg
    10321 cccgcttcct gcagacgttc tgccagttcc ggacgtgccg ggttaactgc gataccgtgg
    10381 tctgtgacca gaatatcgac actggagcct ggggtgatgc aggtcagcac gttatccacc
    10441 agagtcggaa tacgaccgcg taccagcggc gcgacgatga tggaaagcgc agaggcaatc
    10501 gcggtatcgc agtgaccacc ggaagcacca cgcagtacgc cgtcagagcc ggtcagcacg
    10561 ttaacgttga actgggtgtc aatttccagc gcgctcagta ccaccacgtc gagacgatca
    10621 accgatgcgc ctttcgaacc ccagttagcg tactggttgg cgctgatttc gatgtgattg
    10681 gggttacggg ccagcgattg cgcagcatgg ctgtcaaagc tctgcacatc cagcagtttg
    10741 cggatcagac ctttttcgtg cagatcaacc atcgtcgcgg taataccgcc gagggcgaag
    10801 tcggcgcgaa tatcgcggct acgcattttg tcttccagga aacgggttac cgccagcgat
    10861 gcgccgccgg tgccggtttg catggagaaa ccttctttga agtagccaga gttgacaatc
    10921 acatccgcag cgctacgggc aataagcagt tcgcgcgggt tagtggtcat acgggtcgcg
    10981 ccagcgccga tttttgcagc atcgccaacg cggtcaactt tgacgatcaa atcaacctga
    11041 tcttgctcaa tgcttgccgg attatgcgga taaggcagca gttcttcggt aagcatcaca
    11101 acctgttttg cgttgtcggc atcaaccatt gcatagccga gggagccgca gcaggcttta
    11161 ccggtgtaac cgttggcatt accgaattca tcacaggacg ggacgccgag gaaagccacg
    11221 tcgatattca gttcgccgct ctgtaccaga tgcacacgtc cgccgtgaga gtggatttgc
    11281 accggttccg caagcagacc acgggaaatt tcttctgcca gtggaccgcg caggccggag
    11341 gtataaatgc gggtaaccac gccctggcga atgtgttcta ccagtggcgc atggcaatca
    11401 ctcagggagc tggacgccag ggtcaggttt ttaaagccca tcttagcgat gacgtccatc
    11461 accatattga cggtcaggtc accgccacgg aaagcgtgat ggaaggaaac cgtcatgccg
    11521 tcctgtaaac cagagcgacg aatcgcttct tcctggttgg cgcacagttt gcgatcgcgc
    11581 gctttttcag cctggtaggt ttgctttggt gagttctgga aagcggcaag atcgcattca
    11641 gcgcgacgat tccaggccgc tacccgttct tgtcgttgag attgttcaat tttctgcgtc
    11701 attttgattg ccttattctt cgcggatgcc ggaaagttct gcacgggaga gcaccagacg
    11761 ggcacgatcg ataaccggac cgtccaccat cttgccgttc agggaaacca cgccgaggcc
    11821 ttcgcgagcg gcggcttcag cggcttctac gacgcggcgg gcgtgatcca cttctttctg
    11881 ggtcggtgcg tagaggttgt gcaacagatc aatctgacgc gggttgatca gcgatttgcc
    11941 gtcaaagccc agctgtttga tgtgggcggc ttcttgcagg aatccggctt cgttgttagc
    12001 gtcggaatag acggtatcga acgcctgaat acccgcagag cgcgcggcct gcaaaatgga
    12061 acagcgtgcg aacagcagtt cagttccttc cggggagcgt tctgtacgca ggttgcgcac
    12121 atagtcttct gcaccgaggg cgataccgat caaacgctcg gaagcgtgag cgatttccac
    12181 tgcgcgggta atccccagcg gagattcaat cgccgccagc aggccggtgc tgccgggttc
    12241 acgaccacag gctttttcga tacgcaggat ctctttttcg atatccagaa catcctgagc
    12301 ggtatcggtt ttcggcagac gcacaacgtc cgcaccaccg cgaacgacgg cttccaggtc
    12361 gttgacaccc cattcggaat ccagcgcgtt gacacgcaca atggtttcaa tatcgcgata
    12421 cagcggatgt tgcagcgcgt ggtaaaccat gcggcgggcg gtgtcttttt cacgcaatgc
    12481 tacggagtct tcgaggtcaa acatcagggc atcagccggg tagatgaagg agttgctgac
    12541 catcgcggca ttggcaccag gcacaaacaa catgctgcgg cgggtgcgag ttttacgttg
    12601 ttgcagcgaa gcggaaatca ttggcaatcc tcccatggca gagccgggat accgctggcg
    12661 cgtgccagca gggcttccag tcgtgcacgt aaaatgcagt ccagtgcgcc tttgtcatcg
    12721 acattcagct gtacgccgcg cacgttgtag cgggcgagaa cgtccagaat ggtggtgcga
    12781 attgcatcgc caaactgttt ctcaacgctg ctattgattt gcaggtcgat atcctgcgta
    12841 tcgagtgggg cgatgcgtat catcacatcc ccagactcaa gggtgcctgc aacggcgggc
    12901 tggtttattt tcatttttca cctgtttctc atgcgggggt cttttgacga gctgccgcgt
    12961 cctggcggga gtgctcaagc aggttctgca aataatgcag cgtgactgca gggaccagcg
    13021 gcgcgatagc cgtgagatcg tttttcgcca gcagttgacg tacccgggaa gcggatatcg
    13081 gcatctcctg gtaacgcagc cgctcaattt caaccagttc gatgggcggt gcggagatag
    13141 tcggcgtttc cagccagtag cgcatatcct ggttgtactg ggcggtaacg cgacaaaagg
    13201 gttcagtacc gacaaagcgg tgagttacac ccagcgcggg agcgaggtac tgacggaaaa
    13261 tcttcagatc aatttcggtg taacaatggt taatgacgct ctgttcttta atgaagtagc
    13321 aagggaacgt agcgcgggag atgatgtatt cggagccgcg atgcacagtc aggcgtggaa
    13381 tatcggcggt gccttttaac accagatcca accggtcttc ataggggaag cgtgaagaat
    13441 cttctttgac taaaaacaga tgcaaccagt cgcactgtgc cgcagcttgt tgaatcagat
    13501 aacggtgacc attcgtgaag ggattggcgt tcatcacaat gcagccaatc ttgttccctg
    13561 gatgacgaaa ttttttcagc gattcggcat agcgtttcag ccgcgtggcg ctgttttcca
    13621 tcagcaccat cacgccgggt acgctggtca gcgtggaaaa accgcactgg cggaacagcg
    13681 cctcgtattc ggttttggta taaataaaca gatgcgtgct gtgccgctca taggcgaggt
    13741 ttatcaattc agtggctaat gtcagcgcca gtccttcacc gcgaacggat tcactgatag
    13801 caacgcattt aataatattt ccggca
//
LOCUS       DAJTYZ010000109        13443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000109 DAJTYZ010000000
VERSION     DAJTYZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.109"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(206..337)
                     /locus_tag="OO596_003070"
                     /pseudo
     CDS             complement(206..337)
                     /locus_tag="OO596_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            338..883
                     /locus_tag="OO596_003071"
                     /pseudo
     CDS             338..883
                     /locus_tag="OO596_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(925..1926)
                     /gene="gap"
                     /locus_tag="OO596_003072"
     CDS             complement(925..1926)
                     /gene="gap"
                     /locus_tag="OO596_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6718866.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2115..2645
                     /gene="cybB"
                     /locus_tag="OO596_003073"
     CDS             2115..2645
                     /gene="cybB"
                     /locus_tag="OO596_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6718867.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            2890..3063
                     /locus_tag="OO596_003074"
     CDS             2890..3063
                     /locus_tag="OO596_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718868.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3135..3284)
                     /gene="hokB"
                     /locus_tag="OO596_003075"
     CDS             complement(3135..3284)
                     /gene="hokB"
                     /locus_tag="OO596_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6718869.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3683..5323
                     /gene="trg"
                     /locus_tag="OO596_003076"
     CDS             3683..5323
                     /gene="trg"
                     /locus_tag="OO596_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6718870.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5361..6284)
                     /locus_tag="OO596_003077"
     CDS             complement(5361..6284)
                     /locus_tag="OO596_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6718871.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6501..7844
                     /locus_tag="OO596_003078"
     CDS             6501..7844
                     /locus_tag="OO596_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6718872.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8069..9724
                     /gene="opgD"
                     /locus_tag="OO596_003079"
                     /gene_synonym="mdoD"
     CDS             8069..9724
                     /gene="opgD"
                     /locus_tag="OO596_003079"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6718873.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYALKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            9864..10088
                     /locus_tag="OO596_003080"
     CDS             9864..10088
                     /locus_tag="OO596_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6718874.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10151..10687
                     /gene="rimL"
                     /locus_tag="OO596_003081"
     CDS             10151..10687
                     /gene="rimL"
                     /locus_tag="OO596_003081"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6718875.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10682..11662)
                     /locus_tag="OO596_003082"
     CDS             complement(10682..11662)
                     /locus_tag="OO596_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR6718876.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11786..12778
                     /gene="tehA"
                     /locus_tag="OO596_003083"
     CDS             11786..12778
                     /gene="tehA"
                     /locus_tag="OO596_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6718877.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12775..13368
                     /gene="tehB"
                     /locus_tag="OO596_003084"
     CDS             12775..13368
                     /gene="tehB"
                     /locus_tag="OO596_003084"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6718878.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3439 a         3121 c         3412 g         3471 t
ORIGIN      
        1 tactgacatg ggcgactcct gtgatttata tgtttgtttt cggtgaatat attgttaatt
       61 aacatcaatc atcagagaga cggcatcggg aaaggacaat tctgtgaagg gcttcataaa
      121 aacaagtgta aaaggaataa aaggcagaaa taaaagtcag gagtcatgcc cgtttgcaaa
      181 caagtggttc acaaacgggc aatttttaac gtaacgagcg actttccatc gcctcgatga
      241 gcaccgcatc atgtttcagc gtttgccatg catggatgac ttctgccgga aaatcaacgt
      301 gaacgataaa atctcgcccg cggggaccgt agccattgat cagttcgtca atcggctggg
      361 tgaagcgatg caggcggtta aatttggtaa ccccgctgaa cgcaacgaca ttgcgatggg
      421 gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa aaagtggcgc gcgcagtaga
      481 agaaggggcg agagtggcgt tgggtggcaa agcggtagag gggaaaggat attattatcc
      541 gccgacattg ctgctggatg ttcgccagga tatgtcgatt atgcatgagg aaacctttgg
      601 cccggttctg ccggtggtcg catttgacac gctggaagag gctatctcaa tggctaatga
      661 cagtgattac ggcctgacct catcaatcta tacccaaaat ctgaacgtcg cgatgaaagc
      721 cattaaaggg ctgaagtttg gtgaaactta catcaaccgt gaaaacttcg aagctatgca
      781 aggcttccac gccggatggc gtaaatccgg tattggcggc gcagatggta aacatggcct
      841 gcatgaatat ctgcagaccc aggtggttta tttacagtcg taatgagtaa aagaggcgga
      901 ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat ttttcgaggg tgcgaataag
      961 ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc gttttcacca gttgtaaatc
     1021 gcccacggcg gtaatttccg tttgcgtggc atcaaacacc gaaccgaaat ggctgccaat
     1081 gatatcggaa gagactattt cttcatcggt ataaccaaat gactcgttat tggtggttgc
     1141 ttgtttaagt gcgttattca cctcttcggc agtcactttt tttccgagaa tcgataccag
     1201 ttcagtgacc gaacctgttt tcaccggcac gcgttgcgca tgacctttca gtttgccgct
     1261 cagttccggg atcaccagac caatggcttt tgccgccccc gtagtgtggg gaatgatatt
     1321 ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgt gggccatcca ccagtgactg
     1381 ggtgccagta taggcatgaa tggtcgtcat cgtgccgact tctatcccga aactgtcatg
     1441 caaggctttg gccatcggcg caagacagtt agtggtgcat gacgccacgg aaacaatggt
     1501 gtcgttgcca tccagagtgt cgtcattgac gttataaacg atagttttca tttcaccggc
     1561 aggggcggaa atcaacacct tcttcgcacc agcatcaaga tgtgcctgcg atttctcggc
     1621 ggaggtataa aagccagtac attcgacaat gatttctgca cctttcgctt tccacggaat
     1681 atttttagcc tctttttcgg cgtaaaccgc gatacttttc ccatcaacga taagtgaatc
     1741 ttccgtaaaa tcaacgctcc aggggaatgg tccgtagttt gaatcatgtt tcagcaggta
     1801 ggcgagaatt tttggggaag tgagatcatt aatagcgaca acgtctatgt tgcttttgac
     1861 ttcaagtaat cgacgcaaca ccagtcgacc gatacgacca aaaccgttaa taccaacttt
     1921 actcatggtt ttctcctgtc aggaacattc ggatgaaaat tgatcctttc caggcttaga
     1981 ccaggattgt gggatgggca atctccattc ttacagtgaa acgtaacgta ctgaaaacgg
     2041 gtgaacaata tttaatgaaa ttttgagaaa agcccgttat gttaacggaa aattatgtta
     2101 aagcaggaaa tgttatggaa aataaatact caaggttaca aatcagcatt cactggctgg
     2161 tctttttgct ggttatcgca gcgtattgcg caatggagtt tcgtggtttc ttcccacgta
     2221 gcgatcggcc actcatcaac atgattcatg tttcctgcgg catctcaatt ctcgtgctga
     2281 tggtcgttcg tcttttgtta aggctgaaat acccaacccc gccgattata cctaagccaa
     2341 aaccgatgat gacgggactg gcgcatttgg gacatttggt gatgtatctc ctgtttattg
     2401 cgctgccagt gattggtttg gtgatgatgt ataaccgggg caacccgtgg tttgcgtttg
     2461 gtttgacgat gccttacgct tcagaggcca atttcgaacg ggttgatagc ttaaagtcgt
     2521 ggcatgagac gctggcgaat ctgggatatt ttgtcatcgg gttgcacgct gcggctgcac
     2581 tggcacacca ctatttctgg aaggacaaca cacttctacg catgatgccg cgtaaacgtt
     2641 cctgaaggat atttcaagaa aatgcctgta ctaaaaccga cccgtggtac aggcgaagaa
     2701 tacgggtcta catcggaagc gcctattata tttatttgta tgacaaataa aaccataatc
     2761 cttgcccata cgtccatctg gcttattttt aatcaattca cccgatcttt gatctcatca
     2821 acggtatcaa aataaaccag cgtataacgt tcaaatcgaa cataagattc gataaaccat
     2881 ggaggttata tgaaaaaact ggcacttatt ttgtttatgg gaacgcttgt ttccttttat
     2941 gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg ggatctcaca ctgtacggca
     3001 ggcggcaaat ttgtctgtaa tgatggttct attagtgcat cgaaaaaaac atgcactaac
     3061 tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta gtcagatgcg cggggaaaat
     3121 ccccgcgctt gcccttacct ggacgtgcag gccatgagcg cagcaacctc cttatcaccg
     3181 tcccggaacc gcagttcgta gagcgtttgt cgggtcagga gtgtgaatgt cagaatcgta
     3241 atgcagataa tgagcagaca cactaccaga gggttgtgct tcatagcctt tctccttgcc
     3301 ggatggcggg taagaggcta agatctgaat tgctaggttc attcgttggc ctcggttgat
     3361 agaaatatcg gtcggggcct tcgtctttct gattcccggt tagcctgaaa acagaaagtc
     3421 tcaggcaccc gcaggcatcc tatgaggttt ccttagggac gaaaataatc acttcacgaa
     3481 attgcgtgct gttttccaga atttttcgtc attcgggtta gccagtttag ccattcgtta
     3541 ctctcttcat tccaatagca ttaattttct ctgcaataat tgttgtaaaa atgtgacgca
     3601 aagaggtttt tggtcataag taattaccgt caagtgccga tgactttcta tcaggagtaa
     3661 acctggacga gagacaacgg taatgaatac aactccctca cagcgattag gttttttgca
     3721 tcacatcagg ctggttccgt tatttgcctg cattcttggc ggtatcttag ttctattcgc
     3781 attaagttct gctctggctg gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt
     3841 tactgcggag attgagattc ggaccgggtt agcgaacagt tcagattttt tgcgttcagc
     3901 ccggatcaat atgattcagg ccggggctgc gagttgtatt gcggaaatgg aagcaatgaa
     3961 gcgaaatatt gcgcaagccg aatcggagat taaacagtcg cagcaagggt atcgtgctta
     4021 tcagaatcgg ccggtgaaaa cacctgctga tgaagccctc gacactgaat taaatcaacg
     4081 ctttcaggct tatatcacgg gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt
     4141 tgaagcgatt atcaatcatg aaagtgagca gatccgaccg ctggataatg cttataccga
     4201 tattttgaac aaagccgtta agatacgtag caccagagcc aaccaactgg cggaactggc
     4261 ccatcagcgc acccgcttgg gtgggatgtt catgattggc gcgtttgtgc ttgccctggt
     4321 catgacgctg ataacattta tggtgctacg tcggatcgtc attcgtccac tgcaacatgc
     4381 cgcacaacgg attgaaaaaa tcgctagtgg cgatctgacg atgaaggatg aaccggcggg
     4441 tcgtaatgaa atcggtcgct taaatcgtca tttacagcaa atgcagcatt cactggggat
     4501 gacagtaggg actgttcgac agggtgcgga agagatttat cgtggcacca gcgaaatttc
     4561 agctggcaat gcggacctgt catctcgcac cgaagaacaa gcggcggcta tcgaacaaac
     4621 tgccgccagc atggagcaac tcactgcgac ggtgaaacag aatgcggata acgcgcatca
     4681 tgccagcaaa ctggcgcaag aggcttctat taaagccagc gatggcgggc agacggtttc
     4741 cggtgtagta aaaacgatgg gcgctatctc cacgagttcg aagaaaattt ctgagatcac
     4801 cgccgtcatc aacagtattg ctttccagac gaatattctg gcactgaatg ctgccgttga
     4861 agccgcgcga gcgggtgagc aagggcgtgg atttgccgtt gtcgccagcg aagtacggac
     4921 actcgcaagt cgcagcgctc aggcggcgaa agagattgaa ggcttgatca gtgaatcagt
     4981 caggttaatt gacctggggt cggatgaggt ggcaacggcc gggaaaacca tgagcactat
     5041 tgttgatgcc gtcgcgagtg tcacacatat catgcaggaa atcgccgccg cctcggatga
     5101 acaaagtaga ggcataacgc aggttagcca ggcgatttct gaaatggata aggtgacgca
     5161 acagaatgct tctctggtag aagaggcctc agcggcggcg gtgtcccttg aagaacaggc
     5221 ggcacgatta actgaggcgg tggatgtatt ccgtctgcac aaacattctg tgtcggcaga
     5281 acctcgcgga gcgggtgaac cagttagttt cgctacggtg tgaaaatgtt caaggaggga
     5341 tcgacagatc ccttcacctt tcagaacggc attgattttc gaatagcgtt aatcatcaac
     5401 tggcaaccag aagagaacgt cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca
     5461 ccatgtcccg gaacaggcag ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg
     5521 ccagaaggga caaaccacac gtaatcgtat tcaaccgtaa gttgacgaga tagcgaagca
     5581 gacagcgttt cgatacaacc cgaaggaatt ttacagccct ggctctgcac taatgcatct
     5641 gaatgctggc gtggcgcagt gccttctggt gatacaacga ccggccattc cagcacccgg
     5701 cttagcgtta cgttctcctg aagtagcggg tgattagggc ggacaaccag cttcagcgat
     5761 tcaagaaaca gcagttcgta attaagcccg gtcatcagtt caggatctga catccgacca
     5821 atgccgatat cgatttcccc ggtttttaaa cccgccagaa tcatagggtt actcattgtc
     5881 gcaacttgca aggtcgtctc tttttgttgc tgatgaaact gacctataac cgaaggtaat
     5941 atccccagtg ccgcagtagg tagtgcacca accctgacga catcattatt aagaccttct
     6001 ttacgatgaa gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt
     6061 aaaaattgtt cgccgggtaa ggtaagttgc gccccctgac gaccacgctc aaacaagcga
     6121 gcgcccgtca gctgctccag ttcattcaat gtcttagaga gcgcaggttg actcaaatta
     6181 agggtttcag ccgcgcgccc caaagttcct tgttgtgcga cagctacgaa tgtatgaagg
     6241 tggcgcaaac ggatgcgctg actaaacaga ctattttttt ccataagcga tgttaaaaac
     6301 gaagcggtgt cgctgacaag tgaagttgtt tgattatgat aacttgattg caaaatatta
     6361 ttaacaatta aagcaattat gttacagcaa aatggataat attgatgttt tcgcggcgag
     6421 atcacagttt gtaaattctt cccgccagag tgaatgcggt tacctacact ccagattact
     6481 gaccactgga ggcagacact atggcgaaca gcatcacggc ggatgagatt cgggaacagt
     6541 tttcgcaggc aatgtcagcc atgtaccagc aagaagttcc gcaatatggc acgctgctgg
     6601 aactggtagc tgatgtgaat ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa
     6661 tggtaaatgc agacgagctg gcgcgactga atgttgaacg tcatggggcg attcgcgttg
     6721 ggactgcaca agagcttgct actcttcggc ggatgtttgc cattatgggg atgtacccgg
     6781 tgagctatta cgatctctcg caggcagggg tgccggtaca ttcgacagca tttcggccca
     6841 ttgatgatgc ttctctggcg cgtaatccct tccgcgtttt tacctcctta ctccgccttg
     6901 agcttatcga gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt cagcgcgata
     6961 tcttcacccc acgttgtcga caactgttag aggaatatga gcagcagggc ggttttaacg
     7021 aaacacaggc acaggagttt gtgcaggaag ccctggaaac gtttcgctgg caccagtcag
     7081 caacggtaga tgaagaaacc tatcgcgcct tgcacaacga acatcggttg attgctgatg
     7141 tggtctgttt tcctggatgc catatcaacc acctgacgcc acgtacgctg gatattgacc
     7201 gggtgcagtc gatgatgcct gaatgcggaa ttgaacccaa aattctcatc gaggggccgc
     7261 cgcgccgcga ggtaccgatt ttactacgcc agaccagctt taaagcactg gaagagacgg
     7321 tgttgtttgc ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa attgagcagc
     7381 gtggcgtggc attaacgccg aaagggcgac aactgtatga tgatcttctg cgtaacgctg
     7441 gaaccgggca ggataatctc actcaccaaa tgcatttaca ggaaaccttc cgcacttttc
     7501 ctgacagtga gtttttaatg cgtcagcaag ggttggcatg gttccggtac cgtctgacgc
     7561 cttcgggtga ggcgcatcgt caggcgattc atcctggaga cgatccacag cccttaattg
     7621 aacgtggttg ggtagtggcg caacccatca cctatgaaga tttcttgccc gttagcgcgg
     7681 cggggatctt ccagtcaaat ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca
     7741 gtcgcgaagc atttgagcag gcgttgggtt gtccggtttt ggatgagttc cagctttacc
     7801 aggaagcgga agaacgcagt aaacgtcgct gtggtttgct ttaaattctg accatccgcc
     7861 tttgcaaaaa tttgcctgat tttacaaacg aatcaggctc atcccatcga cataaaaaaa
     7921 atgccgattt atgcatattc tctcagttca acaattggat tattaataaa tattgtctag
     7981 agtgagcggt cataaataag cactttcttg ccgctgaaaa cgaccagcgc gggaccattc
     8041 acaacaccag aaggactcac tttcaggtat ggatcgtaga cgatttatta aaggttcaat
     8101 ggctatggcc gccgtgtgcg gtaccagcgg cattgcttct cttttttctc aggcggcatt
     8161 cgcggcagat tctgatattg ccgacgggca aacccagcgt tttgacttct ccattctaca
     8221 gtcaatggcg cacgacttag cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga
     8281 cacgctggcg acaatgacgc cgcaggctta taacagtatt caatacgacg ccgaaaaatc
     8341 gctctggcat aacgttgaga accgtcaact ggacgctcag ttcttccata tgggaatggg
     8401 attccgtcgc cgcgttcgta tgttttctgt agatccagca acacatctgg cgcgtgaaat
     8461 tcactttcgc ccggagttgt tcaaatacaa cgatgcaggt gttgatacca aacaattaga
     8521 agggcaaagc gatctcggct ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg
     8581 ccgtgatgta gtatcatttc tcggcgcgag ttatttccgc gccgttgatg atacatatca
     8641 atacggtttg tcggcccgcg gcctggcgat cgacacttac accgacagta aagaagagtt
     8701 ccccgacttt accgccttct ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt
     8761 ttatgcgttg ctcgatagcg ccagtattac tggtgcctat aagttcacta tccattgtga
     8821 gaaaagtcag gtgattatgg atgtggaaaa tcacctgtat gcgctcaaag acattaaaca
     8881 gctgggcatt gcgccgatga ccagtatgtt cagctgcggt actaatgaac gtcggatgtg
     8941 cgacaccatt catccgcaaa ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg
     9001 cgagtggatt tgccgtccgc tgaacaatcc gcaaaaattg cagttcaatg cttacaccga
     9061 caacaacccg aaagggtttg gtttattgca actggatcgt gacttctccc attatcagga
     9121 cattatgggc tggtataaca aacgcccaag tttgtgggtg gaaccgcgta acaagtgggg
     9181 taagggcacc atcggcctga tggaaatccc aacaacgggc gaaacgctgg ataacattgt
     9241 ctgcttctgg cagccagaaa aagctgtaaa agcaggtgat gagtttgcat tccagtatcg
     9301 tctgtactgg agtgcgcaac cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg
     9361 taccggcatg ggcggtttcc cggaaggttg ggcgccaggt gaacactatc ccgaaaaatg
     9421 ggcgcgtcgt tttgccgtcg atttcgttgg tggtgatctg aaagctgccg cgccaaaagg
     9481 cattgagccg gtgattacgc tttccagtgg ggaagcgaag caaatcgaaa ttctctatat
     9541 tgaacccatc gatggttatc gtattcagtt tgactggtat ccgacttcgg actctactga
     9601 tccggtcgat atgcggatgt atctacgttg tcagggggac gctatcagtg aaacatggct
     9661 gtatcagtat ttcccgccag cgccggataa acgtcagtat gttgacgacc gcgtgatgag
     9721 ttaatcgttt tttcttcggc accttcttcg ggaggtgccg tctggttaaa cacgatcccg
     9781 ctcgcatttt tccctaagtt aaatgagtaa tctgatggtg tgtatttcag atacaccttg
     9841 tcagccacta acagggagtg cgtatgtttc cagaataccg agatttaata tcccgtctga
     9901 aaaacgaaaa tcctcgcttt atgtccttgt tcgataaaca caataaactt gatcatgaaa
     9961 ttgccagaaa ggaaggttcc gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa
    10021 aacaaaagct acagttaaaa gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag
    10081 aggtgtaaac tttcctaagc cgccagccag gcggcttttt taacaacagc atggattgac
    10141 tggagataag atgactgaaa cgataaaagt aagcgaatca cttgaattac atgctgttgc
    10201 ggaaagtcac gtcacacctc tttatcagtt aatctgtaaa aataaaacct ggttacagca
    10261 gtcgctaaac tggccgcagt tcgttcagag tgaagaggac acgcgaaaaa cggtgcaggg
    10321 taatgtgatg ttgcatcaac gcggctatgc caaaatgttc atgattttca aagaagatga
    10381 acttatcggc gttatctcgt ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg
    10441 ctactggctg gacgaatctc atcaggggca ggggatcatt tctcaggcgc tgcaggcatt
    10501 gattcatcat tacgctcagt ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga
    10561 caatccgcaa agcaaccagg tcgctttgcg caatggtttt atccttgaag gttgcctgaa
    10621 acaggctgag ttcctgaatg atgcctatga tgatgtgaac ttatacgcgc gtattatcga
    10681 ttcataataa ccctgccagc ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg
    10741 gaccacggac atgaaccgtg tcgccatcaa aggcttcaac gacggcgtgg tcggttaact
    10801 ccacatggtt ttcaattatc accgcacctg aaatacggct ctcaccttgt attacaacgt
    10861 gctcgtcgag cagaatcggt ccaccacgta ccacggcatt tccgccaatc aagacatgat
    10921 gttttaacac acaattacct tccacaatgg catattccgc cacctgcgaa ctgtaatgaa
    10981 tcgtcggaat ggcatcttct tctatgccag ctttcacctg cgcgtgaccg tagacttttg
    11041 cgcaatcgca tagccagacg ttgttctctt cattaccttc aacactggca aaatcaaaaa
    11101 cttcggcgcg atgttcaata aaagcatacc ggacgacggc atcgccataa atttgtgcct
    11161 ggtggacaat acgcgaggcg ctaacccttg cgcgatcata aatttggagc aggagttgat
    11221 ggtcgggcgt taagccttgt gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac
    11281 caaatattct acattgcccg taaactaatg agtcacgaat ggtgacactg tcacttatat
    11341 atgcaccctg gctgatttca ctattatcga tccagacgtt atccgttgcg taaacctctc
    11401 cccacagcac gctggtgccg gtgatacgcg tattaccaga aatcaccgtt ccgccgaacg
    11461 caatagcatt ctggtcgtaa atccagcaat ttccttcctg tgctaacacc gtttcgcgat
    11521 cgatccagcc gccagcggtt cctgctatca catcgttaaa atcgctaatg gcgataatct
    11581 gccgtaataa cacacttttt ttagtgccat cttcctgata actaaaggct cgctgttctt
    11641 cacttagacg atatttgcgc atagcgtttt ctcacaggtg acttactata accgtagcaa
    11701 attctgcggc tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa atatacttta
    11761 taaattaaac aaaatgagta agaagatgca gagcgataaa gtgctcaatt tgccggcagg
    11821 ctactttggt attgtgttgg ggacgatagg gatgggattt gcctggcgct atgccagcca
    11881 ggtttggcag gtcagccact ggttagggga tgggctggtg attctggcga tgatcatctg
    11941 gggattatta actagcgcat ttattacccg actcatacgc tttccgcata gcgtgctggc
    12001 ggaagttcgc catccagtgc tgagcagttt tgtgagtttg tttccggcaa cgacgatgct
    12061 ggtggcgatt ggttttgttc cgtggtttcg cccactggcg gtgtgcctgt tcagttttgg
    12121 tgtcgtggtt cagttggctt atgccgcctg gcaaactgcg ggattatggc gcggatctca
    12181 ccctgaagaa gctaccacgc ctggactgta tctgccgaca gttgccaaca actttatcag
    12241 cgcaatggcc tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt taggcgcagg
    12301 cgttttctca tggctaagtc ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga
    12361 attacccacg gcactgcgga catcactcgg cattcagctc gctcctgcgc tggtggcctg
    12421 tagtgcctgg ttgagcgtca acggcggcga gggtgatacg ctggcgaaaa tgctttttgg
    12481 ttatggactg ctgcaactgc tgtttatgct acgtctgatg ccatggtatc tctcccagcc
    12541 atttaatgct tcattctgga gtttctcgtt cggcgtatct gcactggcaa ccaccggttt
    12601 gcatctgggg agtggcagcg aaaatggatt tttccatacg ctggcggtac cgctgtttat
    12661 ctttaccaat tttattattg caatactgct catccgtact tttgcgcttc tgatgcaggg
    12721 aaaattgtta gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc
    12781 attcgtgacg aaaactattt tactgataaa tatgaattaa cccgcacaca ctctgaagta
    12841 ctggaagcgg tgaaagtggt taaaccgggt aaaacgctgg atctgggctg tggcaatggt
    12901 cgtaacagtc tttacctggc agccaatggt tatgatgttg acgcatggga taaaaatgcc
    12961 atgagtatcg ccaatgtaga gcgcattaaa tccattgaaa atctggataa tttacacacc
    13021 cgagtcgttg atctgaataa cctcacattt gatagacagt acgattttat tctttcgact
    13081 gtggtactga tgttccttga ggctaaaacc atcccagggc tgattgccaa tatgcaacgt
    13141 tgcactaaac ctggtggtta caacctgatt gtggcggcga tggataccgc tgattatcca
    13201 tgtaccgtcg gcttcccgtt tgccttcaaa gagggagaat tacgtcgata ttacgaaggc
    13261 tgggagaggg tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga cgccaacggt
    13321 aatcgtatta aactgcgttt cgccacgatg ctggcacgta aaaaatgacc cgctaagaat
    13381 aaggcgcgtg aaaattcccc acgctgagat gatttactgt tcttcttttc ggtaagcata
    13441 ttt
//
LOCUS       DAJTYZ010000110        13262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000110 DAJTYZ010000000
VERSION     DAJTYZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.110"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="OO596_003085"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="OO596_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6718879.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OO596_003086"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OO596_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6718880.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OO596_003087"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OO596_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6718881.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAIFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="OO596_003088"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="OO596_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6718882.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="OO596_003089"
     CDS             complement(4036..5568)
                     /locus_tag="OO596_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6718883.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="OO596_003090"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="OO596_003090"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6718884.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="OO596_003091"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="OO596_003091"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6718885.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="OO596_003092"
     CDS             complement(8663..10399)
                     /locus_tag="OO596_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6718886.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OO596_003093"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OO596_003093"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6718887.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="OO596_003094"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="OO596_003094"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6718888.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="OO596_003095"
     CDS             complement(12121..12855)
                     /locus_tag="OO596_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6718889.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13262
                     /locus_tag="OO596_003096"
     CDS             13056..>13262
                     /locus_tag="OO596_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6718890.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVV"
BASE COUNT         3366 a         3370 c         3489 g         3037 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 atcgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
    13261 tg
//
LOCUS       DAJTYZ010000111        12851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000111 DAJTYZ010000000
VERSION     DAJTYZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.111"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..685)
                     /locus_tag="OO596_003097"
     CDS             complement(<1..685)
                     /locus_tag="OO596_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6718891.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMA"
     gene            941..1096
                     /gene="rseD"
                     /locus_tag="OO596_003098"
     CDS             941..1096
                     /gene="rseD"
                     /locus_tag="OO596_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6718892.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1093..1668
                     /gene="rpoE"
                     /locus_tag="OO596_003099"
     CDS             1093..1668
                     /gene="rpoE"
                     /locus_tag="OO596_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6718893.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1701..2351
                     /gene="rseA"
                     /locus_tag="OO596_003100"
     CDS             1701..2351
                     /gene="rseA"
                     /locus_tag="OO596_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6718894.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2351..3307
                     /gene="rseB"
                     /locus_tag="OO596_003101"
     CDS             2351..3307
                     /gene="rseB"
                     /locus_tag="OO596_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6718895.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3304..3783
                     /gene="rseC"
                     /locus_tag="OO596_003102"
     CDS             3304..3783
                     /gene="rseC"
                     /locus_tag="OO596_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6718896.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3981..5780
                     /gene="lepA"
                     /locus_tag="OO596_003103"
     CDS             3981..5780
                     /gene="lepA"
                     /locus_tag="OO596_003103"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6718897.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5796..6770
                     /gene="lepB"
                     /locus_tag="OO596_003104"
     CDS             5796..6770
                     /gene="lepB"
                     /locus_tag="OO596_003104"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6718898.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7043..7723
                     /gene="rnc"
                     /locus_tag="OO596_003105"
     CDS             7043..7723
                     /gene="rnc"
                     /locus_tag="OO596_003105"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6718899.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7720..8625
                     /gene="era"
                     /locus_tag="OO596_003106"
     CDS             7720..8625
                     /gene="era"
                     /locus_tag="OO596_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6718900.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8637..9365
                     /gene="recO"
                     /locus_tag="OO596_003107"
     CDS             8637..9365
                     /gene="recO"
                     /locus_tag="OO596_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6718901.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9377..10108
                     /gene="pdxJ"
                     /locus_tag="OO596_003108"
     CDS             9377..10108
                     /gene="pdxJ"
                     /locus_tag="OO596_003108"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6718902.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10108..10488
                     /locus_tag="OO596_003109"
     CDS             10108..10488
                     /locus_tag="OO596_003109"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6718903.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10710..10790
                     /gene="shoB"
                     /locus_tag="OO596_003110"
     CDS             10710..10790
                     /gene="shoB"
                     /locus_tag="OO596_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6718904.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10984..11244)
                     /gene="yfhL"
                     /locus_tag="OO596_003111"
     CDS             complement(10984..11244)
                     /gene="yfhL"
                     /locus_tag="OO596_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6718905.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11459..12649
                     /locus_tag="OO596_003112"
                     /pseudo
     CDS             11459..12649
                     /locus_tag="OO596_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         3277 a         3134 c         3346 g         3094 t
ORIGIN      
        1 acgccatagc aatgccatcg ccagaagaaa tatccggatt ggtggtgtac tgatacacct
       61 tcgacgcacc gccggttgcc agtaccactg cttttgcgtg gcaggtttcc accgtttctt
      121 tatttcggtt ccatacccac gcgcctacaa cccgtcgcgt gcccggcagg ccaattttgt
      181 cagagacaat cagatcaact gcgttgctgc gctccagcac acgaatattc ggatggttct
      241 gcgccttgct caccagcgtg gattgtactt ctctaccggt ggcgtcggcg gcatgaagaa
      301 tacgacggtg actatgtcca ccttcacggg tcagatggta gctttcttcg ccattcggtt
      361 gaacgtgggt atcaaacaaa accccctggt caattaacca ttgcacacag gaacgagcat
      421 tgctggcgac aaattcaact gcatggcgat cgcaaatacc agccccggca atcaatgtgt
      481 cttccacatg cgagtcaatg ctgtcagttt catcaaacac ggcggcaata ccgccctggg
      541 cataaaatgt tgaaccttcc gttaccgggc ctttacttag aacgatgacc tgatgctggt
      601 cagccaggcg tagcgccagt gaaagtccgg ctgcgccgct accaataatc aacacgtcac
      661 atgaatgttc agggagagta ttcattttct ttgtttaatt tactaaacat tgtttggtca
      721 gcatagcatc atgttgtgcg aataaacacc tgctatttta atatttgtta cagtggctaa
      781 acacgttgac gcagggcggc gagaaaaaga gaagttactg gctggtggag gattaggtgg
      841 tgaaataaaa aggccgttgg gttactcttc aggcagttaa atgggcattt ctacacagat
      901 aatgcgatgt tcagattctg tagacttata atgatagata atgatccgtc tacagcatga
      961 caaacaaaaa cagatgcgtt acggaacttt acaaaaacga gacactctaa ccctttgctt
     1021 gctcaaattg cagctaatgg agtggcgttt cgatagcgcg tggaaatttg gtttggggag
     1081 actttacctc ggatgagcga gcagttaacg gaccaggtcc tggttgaacg ggtccagaag
     1141 ggagatcaga aagcctttaa cttactggta gtgcgctatc agcataaagt ggcgagtctg
     1201 gtttcccgct atgtgccgtc gggtgatgtt cccgatgtgg tacaagaagc ttttattaaa
     1261 gcctatcgtg cgctggattc gttccgggga gatagcgctt tttatacatg gctgtatcgg
     1321 attgctgtaa atacagcgaa aaattacctg gttgctcagg ggcgtcgtcc accttccagt
     1381 gatgtggatg ccattgaagc tgaaaacttc gaaagtggcg gcgcgttgaa agaaatttcg
     1441 aaccctgaga acttaatgtt gtcagaagaa ctgagacaga tagttttccg aactattgag
     1501 tccctcccgg aagatttgcg catggcaata accttgcggg agctggatgg cctgagctat
     1561 gaagagatag ccgctatcat ggattgtccg gtaggtacgg tgcgttcacg tatcttccga
     1621 gcgagggaag ctattgataa caaagttcaa ccgcttatca ggcgttgacg atagcgggat
     1681 actggataag ggtattaggc atgcagaaag aacaactttc cgctttaatg gatggcgaaa
     1741 cgctggatag tgagctgctt aacgaactgg ctcataaccc agaaatgcag aaaacctggg
     1801 aaagctatca cttaatccgt gattcaatgc ggggtgatac tcccgaggtg ctccatttcg
     1861 atatctcttc acgcgtgatg gccgccattg aagaagagcc agtacgtcaa ccggcgacat
     1921 tgatcccgga agcccagcct gcgccgcatc aatggcacaa aatgccattc tggcagaaag
     1981 tacgtccgtg ggcggcacag cttacccaaa tgggcgtagc agcatgcgta tcgcttgcag
     2041 ttatcgttgg cgtccagcac tataatggac aatctgaaac gtcccagcag cccgaaacgc
     2101 cggtatttaa tacactgccg atgatgggta aagccagccc ggtaagcctg ggagtacctt
     2161 ctgaagcgac cgcaaacaat ggtcaacagc agcaggtaca ggagcagcgt cgtcgcatta
     2221 atgcaatgtt gcaggattac gaactgcaac gccgactcca ctctgaacag cttcagtttg
     2281 agcaggcgca aacccagcaa gccgctgtac aggtgccagg acttcaaact ttaggaacgc
     2341 aatcgcagta atgaagcaac tttggtttgc catgtcatta gtgacaggta gcctgttatt
     2401 ctctgctaac gcctcggcca ctcccgcgtc cggggcgtta ttacagcaga tgaacctggc
     2461 cagtcagtca ctgaattacg agctgtcatt catcagcatc aataaacagg gtgttgagtc
     2521 tctgcgttat cgacatgcac gcctcgataa ccgtcctctt gcacaattgt tgcaaatgga
     2581 tggcccgcgc cgggaagtgg tacagcgcgg caatgaaatc agctattttg aaccgggact
     2641 tgaaccgttc acgcttaatg gcgattacat tgttgattct ctgccatcgc ttatctatac
     2701 cgatttcaaa cgcctttctc cttactacga ctttatctcc gtcgggcgca cgcgtattgc
     2761 tgatcgtctt tgcgaagtca ttcgcgtggt tgcccgagat ggtacacgct acagctacat
     2821 cgtgtggatg gacactgaat cgaaattacc gatgagggtt gatctccttg atcgcgatgg
     2881 tgaaacgctg gaacaatttc gcgtgatttc ttttaacgtc aatcaggata tcagcagcag
     2941 tatgcagacg ctggcgaagg caaatttgcc gccgttgctt tctgttcctg taggtgaaaa
     3001 agctaaattc agctggacgc caacctggtt gccacagggt tttagcgaag tttccagtag
     3061 tcgacgtccg ctaccgacga tggacaacat gcctatcgaa tcacgtctct attccgacgg
     3121 attattcagc ttctcggtaa acgttaaccg cgctacgcca tcgagcaccg atcagatgtt
     3181 gcgcaccgga cgcagaaccg tcagtacaag cgtacgtgat aacgccgaaa tcaccattgt
     3241 cggtgaactg ccgccgcaaa cggcgaaacg cattgccgag aatattaagt tcggggcagc
     3301 gcaatgatca aagagtgggc taccgtcgtc tcctggcaaa acgggcaggc gctggtcagt
     3361 tgtgatgtta aagcctcatg cagcagctgc gcttcacgcg ccggttgcgg tagccgcgtg
     3421 ttgaataaac ttggcccgca aaccacgcat accattgtcg taccctgtga tgaaccgtta
     3481 gtgccggggc aaaaagtgga attggggatc gccgaaggca gcctgcttag ctccgcatta
     3541 ctggtttata tgtcgccgct ggtgggatta ttccttatcg cttcgctatt tcagctactc
     3601 tttgcttctg acgttgcagc attatgtggt gcgatccttg gcggtattgg cgggtttctg
     3661 attgcgcgcg gttactcgcg taagtttgct gcccgggcag aatggcaacc gatcatatta
     3721 agcgtggcat tgccgcctga ccttgtgcga tttgaaacat cttcggaaga cgcgagccag
     3781 tgatttcact ggctttgttg cattttgcat aacaaatgcg aggacgtttc cagataccac
     3841 aatccatacg gtatgaacat ttcctcgcct caatgttgta gtgtagaatg cggcgtttct
     3901 attaatacag acgttaagct cagaacagcg acttctaaaa gcctggttaa ccgggcatta
     3961 aggcacaata atcatacttt atgaagaata tacgtaactt ttcgatcata gctcacattg
     4021 accacggtaa atcgacgctg tctgaccgta ttatccagat ctgcggtggc ctgtctgacc
     4081 gtgaaatgga ggcgcaggtt ctcgattcca tggatcttga gcgtgagcgt ggcattacca
     4141 tcaaagcgca aagcgtgacg ctggactaca aagcgtctga cggcgaaacc tatcagctta
     4201 actttatcga caccccgggc cacgtagact tctcctatga agtttcccgt tcgctggctg
     4261 cctgtgaagg tgcattgctg gtggtcgacg ccgggcaggg cgtggaagcg caaaccctgg
     4321 caaactgcta caccgccatg gaaatggatc tcgaagtggt gccagtactg aacaagattg
     4381 acctgccggc agccgatcct gaacgcgtgg cggaagaaat tgaagatatc gtcggcatcg
     4441 acgccactga cgcggtgcgc tgttcagcga aaaccggcgt tggcgtgcag gacgttctcg
     4501 aacgtctggt gcgcgacatt ccgccgccgg aaggcgatcc ggaaggctcg ttgcaggcac
     4561 taattatcga ctcatggttc gacaactacc tgggcgttgt ttcacttatc cgtattaaaa
     4621 acggcaccct gcgtaagggc gacaaagtga aagtcatgag taccgggcag acctataacg
     4681 ccgaccgtct gggcatcttc acgccgaaac aggttgaccg cactgaactg aaatgtggcg
     4741 aagtaggctg gctggtatgt gcgattaaag atatccatgg cgctccagtc ggcgatacct
     4801 taacgctggc gcgtaatccg gcagaaaagg cgctgcctgg ctttaagaaa gtcaaaccgc
     4861 aggtatacgc cggtctgttc ccggtaagtt ccgacgacta tgaagccttc cgtgacgcgc
     4921 tgggtaaact cagcctgaat gatgcctcac tgttctatga gccggaaagc tccagcgcgc
     4981 tgggctttgg tttccgctgc ggcttccttg gcctgctgca catggagatc atccaggaac
     5041 gtctggaacg tgaatacgat ctggatctga tcaccactgc gccgaccgta gtgtatgaag
     5101 ttgaaaccac gtcaagggaa gttatctacg tcgacagccc atccaagctg cctgcggtaa
     5161 ataacattta cgaactgcgc gagccgattg cagagtgtca catgctgctg ccgcaggcat
     5221 atctcggcaa cgttattacg ctgtgcgtag aaaaacgcgg cgtgcagacc aatatggttt
     5281 accacggtaa tcaggtggcg ctgacgtacg agatcccgat ggcggaagtg gtgctcgact
     5341 tcttcgatcg cctgaaatct acctcgcgtg gttatgcgtc tctggattac aacttcaaac
     5401 gcttccaggc gtccgacatg gtacgtgtag acgtattaat caacggtgaa cgtgttgatg
     5461 cgttggcgct gatcacccac cgtgataatt cgcaaaaccg cggtcgcgag ttggtggaga
     5521 agatgaaaga tctgatccca cgccagcagt ttgatatcgc cattcaggca gcgattggta
     5581 cgcacattat tgcgcgatcc accgtgaaac agctgcgtaa aaacgtactg gctaaatgtt
     5641 atggcggcga tatcagccgt aagaaaaagc tgctgcagaa gcagaaagaa ggtaagaaac
     5701 gcatgaagca gatcggtaac gtcgagctgc cgcaggaagc gttcctcgcc attctgcacg
     5761 tcggcaaaga caacaaataa cccttaggag ttggcatggc gaatatgttt gccctgattc
     5821 tggtgattgc cacactggtg acgggcattt tatggtgcgt ggataaattc tttttcgcac
     5881 ctaaacggcg ggaacgtcag gcagcggcgc aggcggctgc cggtgactca ctggataaag
     5941 caacgttgaa aaaggttgca ccgaagcttg gctggctgga aaccggagct tctgtttttc
     6001 cggtgctggc tatcgtattg attgtacgtt cgtttattta tgaaccgttc cagatcccgt
     6061 caggttcgat gatgccgact ctgttaatcg gtgattttat tctggtagag aagtttgctt
     6121 atggcattaa agatcctatc taccagaaaa cgctgatcga aaccggtcat ccgaaacgcg
     6181 gcgatatcgt ggtctttaaa tatccggaag atccaaagct tgattacatc aagcgcgcgg
     6241 tgggtttacc gggcgataaa gtcacttacg atccggtctc aaaagagctg acgattcaac
     6301 cgggatgcag ttccggccag gcgtgtgaaa acgcgctgcc ggtcacctac tcaaacgtgg
     6361 aaccgagcga tttcgttcag accttctcac gccgtaatgg tggggaagcg accagcggat
     6421 tctttgaagt gccgaaaaac gaaaccaaag aaaatggaat tcgtctttcc gagcgtaaag
     6481 agacactggg tgatgtgacg caccgaattc tgacagtgcc gattgcgcag gaccaggtgg
     6541 ggatgtatta ccagcagcca gggcaacaac tggcaacctg gattgttccg ccgggacaat
     6601 acttcatgat gggcgacaac cgcgacaaca gcgcggacag ccgttactgg ggctttgtgc
     6661 ctgaagcgaa tctggtcggt cgggcaacgg ctatctggat gagtttcgat aagcaagaag
     6721 gcgaatggcc gacaggtgtg cgcttaagtc gcattggcgg catccattaa tagcccatct
     6781 tcgttcacgt tgtcgccgtt atggcgacaa cgtgaattat ttatgagata aatctcccgt
     6841 ggctaacgac atcccccgtc gttgtgtata gaatattccc ccgaagttta aggttggcac
     6901 ctccaggttg ccacggcaca cgaaacagcg ttggtcccca tataccggta aactgaaact
     6961 gcagcgaagc agttagcaga gccatgtata tcaggtctgt ttcgtgtgct gaattgttga
     7021 cgcatttatt tattggtatc gcatgaaccc catcgtaatt aatcggcttc aacggaagct
     7081 gggctacact tttaatcatc aggaactgtt gcagcaggca ttaactcatc gtagtgccag
     7141 cagcaaacat aacgagcgtt tagaattttt aggcgactct attctgagct acgttatcgc
     7201 caatgcgctt tatcaccgtt tccctcgtgt ggatgaaggc gatatgagcc ggatgcgcgc
     7261 cacgttggtc cgtggcaata cgctggcgga actggcgcgc gaatttgagt taggcgagtg
     7321 cttacgttta gggccaggtg aacttaaaag cggtggattt cgtcgtgagt caattctcgc
     7381 cgataccgtc gaagcattaa ttggtggcgt attcctcgac agtgatattc aaaccgtcga
     7441 gaaattaatc ctcaactggt atcaaactcg tttggacgaa attagcccag gcgataaaca
     7501 aaaagatccg aaaacgcgct tgcaagaata tttgcagggt cgccatctgc cgctgccgac
     7561 ttatctggta gtccaggtac gtggcgaagc gcacgatcag gaatttacta tccactgcca
     7621 ggtcagcggc ctgagtgaac cggtggttgg cacaggttca agccgtcgta aggctgagca
     7681 ggctgccgcc gaacaggcgt tgaaaaaact ggagctggaa tgagcatcga taaaagttac
     7741 tgcggattta ttgccatcgt cggacgtccg aacgttggca aatccacatt gttgaacaaa
     7801 ctgctggggc agaagatctc catcacctcc cgcaaagcgc aaacaactcg tcaccgcatt
     7861 gtggggatcc atactgaagg cgcgtatcag gcgatctacg ttgatacccc tggcctgcat
     7921 atggaagaaa aacgcgctat taaccgcctg atgaacaaag cggcgagcag ctctattggc
     7981 gacgttgagc tggtgatttt tgtcgttgaa ggcacccgct ggacgccgga cgacgaaatg
     8041 gtgctcaaca aactgcgcga tggcaaagcg ccggtgatcc tcgcggtgaa caaagtggat
     8101 aacgtgcagg aaaaagccga tctgctgccg cacttacagt tcctggcaag tcagatgaac
     8161 ttcctcgata tcgtgccgat ctctgctgaa accgggctga atgtcgacac tatcgcagca
     8221 atcgtgcgta agcatctgcc tgaagcgacc catcacttcc cggaagatta catcaccgat
     8281 cgctcacagc gttttatggc gtctgaaatc atccgcgaaa aactgatgcg tttcctcggc
     8341 gctgaactgc cgtactccgt gaccgtggag atcgaacgtt tcgtctctaa cgaacgcggt
     8401 ggttatgaca tcaatggttt gattctcgtt gagcgtgaag ggcagaagaa gatggttatt
     8461 ggcaacaaag gggccaagat caaaaccatc gggattgaag cgcgtaaaga catgcaggaa
     8521 atgttcgaag cgcctgtcca ccttgaactg tgggtaaaag tgaaatccgg ttgggccgac
     8581 gacgaacgcg cactgcgcag tctcggttac gttgacgatc tttaagagtc actccgatgg
     8641 aaggctggca gcgcgcattt gtcctgcata gtcgcccgtg gagcgaaacc agcctgatgc
     8701 tggacgtctt cacggaggaa tcgggtcgcg tgcgtctggt tgccaaaggc gcacgctcta
     8761 aacgctctac tctgaaaggt gcattacagc ctttcacccc tctcttgcta cgtttcggcg
     8821 ggcgtggcga agtcaaaacg ctgcgcagtg ctgaagctgt ctcgctggcg ctgccattaa
     8881 gcggtatcac gctttacagc ggtctgtaca tcaacgaact tctctcccgc gtactggaat
     8941 acgagacgcg cttctctgaa ctctttttcg attacttgca ctgcattcag tctcttgcag
     9001 gtgccactgg tacgccagaa cccgcgctgc gccgctttga actggcactg ctcgggcatc
     9061 tgggctatgg cgtcaatttt acccattgcg cgggtagcgg cgagccggta gatgacacca
     9121 tgacgtatcg ttatcgcgaa gaaaaagggt ttatcgcaag cgtcgttatc gacaataaaa
     9181 cgttcaccgg aaggcagtta aaagcgttaa acgcgcggga atttcctgac gcagacacac
     9241 tacgcgccgc gaaacgcttt acccgcatgg cgcttaagcc gtatcttggc ggtaaaccat
     9301 taaagagcag ggaactgttc cggcagttta tgcctaagcg aacggtgaaa acacattatg
     9361 aatgatgagg attgtcatgg ctgaattact gttaggcgtc aacattgacc atattgcaac
     9421 gctgcgtaac gcacgcggta ctgcttaccc ggatccggtg caggcggcgt ttattgccga
     9481 gcaggcggga gcggacggca ttaccgtgca tttacgtgaa gatcgccgcc acattactga
     9541 ccgcgatgtg cgcatcctgc ggcagacgct ggatacccga atgaatctgg agatggcagt
     9601 gaccgaagag atgctggcga tcgccgttga gacgaagcca catttttgct gcctggtacc
     9661 ggaaaagcgt caggaagtaa caaccgaagg cggcctggac gttgcagggc agcgtgaaaa
     9721 aatgcgcgat gcctgcaaac gtctgacaga tgccgggatt cagatttctc tctttatcga
     9781 cgccgatgaa gagcagatca aagctgcggc agaggttggc gcaccgttta tcgagatcca
     9841 caccggttgc tatgctgatg ccaaaactga cgccgaacag gcgcaagagc tggtgcgtat
     9901 cgccaaagcc gcgacctttg ccgctagtct cggtctgaaa gttaacgccg gacacggtct
     9961 gacctatcac aacgtgaaag ccattgccgc catccctgag atgcatgaac tgaatatcgg
    10021 tcatgccatt attggccgtg cagtgatgac cggactgaaa gatgcggtgg cagaaatgaa
    10081 gcgtctgatg ctggaagcgc gtggctaatg gcaatattag gtttaggcac ggatatcgta
    10141 gagatcgctc gcatagaagc agtgatcgcc cgatccggtg agcgcctggc acgccgcgta
    10201 ttaagcgata acgaatgggc aatctggaaa acgcaccacc agccggtgcg ttttctggcg
    10261 aagcgttttg ctgtgaaaga agccgcagca aaagcgtttg gcaccgggat ccgcaatggt
    10321 ctggcgttta atcaatttga agtattcaat gatgagctcg gcaaaccacg gctacggcta
    10381 tggggtgagg cattaaaact ggcggaaaag ctcggcgttg taaatatgca tgtaacgctg
    10441 gcagatgaac gccactatgc ttgtgccacg gtaattattg aaagttaaaa taataccgtt
    10501 ctctttaaaa agtaaaaccc ccgaactggt aaggaacggg ggttttactt tttaacttta
    10561 attttgtgca gcatgcaccc tcaacaacag cgattatgga tcagtgtact tatcttttac
    10621 aagcaacttg caatctttag cataaaaact cgagccttta cgaagaaagc aatactgaac
    10681 ggctgcattc cttgtttaag gaaaaacgaa tgactgattg ccgatacctg attaaacggg
    10741 tcatcaaaat catcattgct gttttacagc tgatccttct gttcttataa cacaaggaaa
    10801 cgtacttaag gtgcgtccgg tgaaccaatc ggacgcgcct ttaataacta taaataagtg
    10861 tcttggcaga tactatataa attaacttag tgaatgatta tgctaatgtc atcaattaaa
    10921 taaatataat ggcgttaagg cttcccagta atataattaa tactctactt ccagagtaga
    10981 atattaaatt ttatccgcgt ggtgcatcag cacaaattta tcccacaact gttcttctgt
    11041 ctcgacatgc gccggatctt tcacaatagt attggggatc gggcacacct tctggcaggt
    11101 tggtgtctcg tagtgcccta cgcattcggt acacttatcg ctgttaatct cgtagatatg
    11161 atctcccatt gaaatcgcct cattcgggca ttcgggttca cacatatcac aattaataca
    11221 gcgtttagta attagtaaag acatttcaat ggattaccgt taaatcattt taaaatcagt
    11281 aagttgtatc gagtttgtat gctttactgt cattaactta ctgtatgttg atccagtgta
    11341 tttaaccttg ataaactcag tccagcaaca caaaaccgca acacattgca ttttgtcccg
    11401 tagaaaagac ttgtatgtgt gagcttgttt tctgcgccta cgcagataag gattgagaat
    11461 gccgcgcact gtaacacata atccggatag ccccaataat gacgatgttt tagccgcatc
    11521 tgaaaaatgg gacgcctgta aaccccccta taccagcgca cacatgaaaa tctgtgttgc
    11581 tgccgccaaa atcatcctcg ctgcttccgg cgtggctcgc cgttccaaat acgaaaaaga
    11641 gaactatctc cgtatcgatt tcagcaaagc cggtaaggtt acattttacg ccgagtttcc
    11701 aaaaaagatg ggcctcaaag gtaaaaagct cggcgagtgg ccggagctcg ctatccagct
    11761 ggcgcgcgaa aaagcactag gtatggctga cggtggactg cgggcagagt ccgtacatgc
    11821 agcgctggaa atgtaccggg atgacctcaa agccaaagta gcccggcaga agctgagccc
    11881 ggacagtttc acaacctacg gggtgcgtat cgaccggatt aaagcaacgt tcggcgagcg
    11941 tgaggtgttc agcgacgtaa catacaatcg gctggtggaa gtgctggacg agtggatcgc
    12001 cactcgctcg aacaataacg ccctggagtt gtttgccgag ctccgtcggt tctggaagtt
    12061 ctgcgcacct actctttgca acggccgcaa tgttgccgcc agtctgccag atgattatgt
    12121 ttcctcccgc gtacagaaac ctacccccac acggcttttt accgatattg aatcaatcgc
    12181 ccgactctgg ctcaatgttg ctgcctgcac ctctgtacac cagaagaatg ctgttcgctt
    12241 catgatcatc actggtgttc gtccgattaa tgtccataac ctgcgctggg actacgttta
    12301 cgaggaggct ggtgaaattg tttatccgga aggggttatc ggcatgcgag gggctatgaa
    12361 aacacaaaag gctttccgcc tgccgataac gcctgagatc cggcggatta tcgacgagca
    12421 gaaagcctgg cgtgattcag ttcctgagtg caacagggat tatgtatttt tgcagccacg
    12481 tgatccaatg cagccatttt caaaacgatc actggataag ctggtgaaaa catacagccc
    12541 ggacggggct gtaaaaggaa taaaacatga tgggactgtt aaagggaaag acggtgcatt
    12601 taatacgatg tgccgtaaat tccttaagag caatgttatt gccttgatcg ataggaatta
    12661 aaaccccaaa aagattaaaa aaacaccaca aaacggatgt ttcttcaaca ccacttttgc
    12721 tcatatgaac ggaaccgacg attaaactgg atggctctga ttgattcagg gtatgaatgg
    12781 cggttttttg ctccgtttcc ctcaaaatgg acgcaacttt ccctctgcgg ctctcagccg
    12841 caccaccgca t
//
LOCUS       DAJTYZ010000112        12794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000112 DAJTYZ010000000
VERSION     DAJTYZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.112"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(427..1518)
                     /gene="ychF"
                     /locus_tag="OO596_003113"
     CDS             complement(427..1518)
                     /gene="ychF"
                     /locus_tag="OO596_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6718906.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1635..2219)
                     /gene="pth"
                     /locus_tag="OO596_003114"
     CDS             complement(1635..2219)
                     /gene="pth"
                     /locus_tag="OO596_003114"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6718907.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2496..2774
                     /gene="ychH"
                     /locus_tag="OO596_003115"
     CDS             2496..2774
                     /gene="ychH"
                     /locus_tag="OO596_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6718908.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2829..4508)
                     /gene="dauA"
                     /locus_tag="OO596_003116"
     CDS             complement(2829..4508)
                     /gene="dauA"
                     /locus_tag="OO596_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6718909.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4632..5579)
                     /gene="prs"
                     /locus_tag="OO596_003117"
     CDS             complement(4632..5579)
                     /gene="prs"
                     /locus_tag="OO596_003117"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6718910.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5730..6581)
                     /gene="ispE"
                     /locus_tag="OO596_003118"
     CDS             complement(5730..6581)
                     /gene="ispE"
                     /locus_tag="OO596_003118"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6718911.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6581..7204)
                     /gene="lolB"
                     /locus_tag="OO596_003119"
     CDS             complement(6581..7204)
                     /gene="lolB"
                     /locus_tag="OO596_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6718912.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7418..8674
                     /gene="hemA"
                     /locus_tag="OO596_003120"
     CDS             7418..8674
                     /gene="hemA"
                     /locus_tag="OO596_003120"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6718913.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8716..9798
                     /gene="prfA"
                     /locus_tag="OO596_003121"
     CDS             8716..9798
                     /gene="prfA"
                     /locus_tag="OO596_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6718914.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9798..10631
                     /gene="prmC"
                     /locus_tag="OO596_003122"
     CDS             9798..10631
                     /gene="prmC"
                     /locus_tag="OO596_003122"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6718915.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10628..11020
                     /locus_tag="OO596_003123"
     CDS             10628..11020
                     /locus_tag="OO596_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6718916.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11024..11832
                     /locus_tag="OO596_003124"
                     /pseudo
     CDS             11024..11832
                     /locus_tag="OO596_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            11868..12722
                     /gene="kdsA"
                     /locus_tag="OO596_003125"
     CDS             11868..12722
                     /gene="kdsA"
                     /locus_tag="OO596_003125"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6718917.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3190 a         3207 c         3321 g         3076 t
ORIGIN      
        1 attcattttt ggtcaatggg ttttctggcc tatcgttgtt attgtgctac tggttatcat
       61 tttcatcttg ccaggatcgc acgtaatcac gttattcggg gactttatct gcgggatatt
      121 acccttcagg taagtccttt tctcgccagc atattggagc gtgatcaata ttgatcagct
      181 gtgaaggggg tgatgtgtag ttgaacaaaa acttacgtta tatcactact aatgagatat
      241 ccgtcatctg acactgatgt ctttaattat tctcgtggtt cactgtaggc aataagcaca
      301 aaagtgtagg atgttacaag aatgattagg actcggtgaa atgaaaaatc cacgcaattg
      361 cgtggatttt atatactttt gcgctcttca tgagatttag cgaaacctca tgagacaata
      421 aattaattag acgttgaaaa ggaagttcat cacatcgcca tctttaacga tgtaatcttt
      481 accttctgca cgcattttgc ctgcttcttt cgcgccttgt tcacctttgt aagtgatgaa
      541 atcttcaaac gagatggttt gtgcacggat aaagcctttt tcaaaatcag tatggatttt
      601 gcccgctgcc tgcggcgcgg ttgctccaac cggaatggtc catgcacgca cttctttcac
      661 cccagcggtg aagtaagttt gcaggttcag cagcttataa ccgacacgga tcacacggtt
      721 caggcccggt tcttccagcc caagctcctg cataaactcg tcacgttctt cgtcgtccag
      781 ttcggcaatg tctgcttcaa cagcagcaca aaccggaacc acaacagaac cttctttcgc
      841 cgcgatttca cgcacctggt caagatatgg gttgttttca aaaccgtctt cgttgacgtt
      901 ggcgatgtac attgttggtt tcagcgtcag gaagctcagg taacgaatag ccgctttctc
      961 ttcagcgctt aaatccagcg cgcgcagcat acctgcgttt tccagctggg gcaggcattt
     1021 ttctaggacc gccagctcag ctttcgcgtc tttatcgcca cctttggctt tcttctgtac
     1081 gcgatgaatc gcacgttcgc aggtgtcgag gtctgccagc gccagttcgg tgttgataac
     1141 ttcaatatcg tcagccgggt taactttgcc ggaaacgtga atgatgttgt cattttcaaa
     1201 gcagcgaaca acgtgaccga tcgcttcggt ttcacggatg ttggtcagga actggttacc
     1261 cagaccttcg cctttcgatg cgccttttac cagaccggcg atatcgacaa attccatggt
     1321 cgtgggaagc gtacgttgcg gttttacgat ttcagccagt tgatccaggc gagggtcagg
     1381 cattggtacg acgcctgtgt tcggctcaat ggtgcagaat ggaaagttgg ccgcttcaat
     1441 accggctttg gtcagcgcgt tgaacagggt agatttcccg acgttgggca aaccgacgat
     1501 accgcatttg aatcccatga tttaaatcac cttaatatct taataatcaa cctgttattg
     1561 ctaacagatt gcagaaatgg aaataacttt gcctattata cacggcactc ggcaaaaatg
     1621 ccgcagacaa cgacttattg cgctttaaag gcgtgcaatc ggttcgttgc tttggtcaag
     1681 ccatctgtaa accacatttc agtacaacgc gccgcttcgt cgatggcttc atcaattaac
     1741 ttctgttcac taacaggcgg tttgcctaac acaaaaccga caactttatt tttatcgcca
     1801 ggatgaccga ttccgatgcg taaacggtga aagttagggt tattacccaa tttactgatg
     1861 atgtctttca gtccattgtg accaccatgg ccaccgccca atttaaattt ggcgacgcca
     1921 ggaggcagat ccagttcgtc gtgggccacc agaatttcgt ccggattaat gcggaaaaaa
     1981 ctggccatcg ccgcaacggc tttgccgctg agattcataa atgtagtcgg gactaacagg
     2041 cggacatctt cgcctccaag agtgactcgc gaagtataac caaagaattt agcctcttcg
     2101 cgcagcggag cgcgcaaacg ctctgccagt aagtcaacga accaggcacc agcattatgt
     2161 cgcgttgcgg cgtattcagc accggggttc gccaggccga caatcaattt aatcgtcacg
     2221 ttttttgtcc tgagtgtgta cataactggc gcgtagttta ctggttgcgg ccccgcttga
     2281 caaaaaactg cgtatcaaat gcagataacg taataattgc ctgagtggac tattagaaag
     2341 tcaaggtgtt caggcgttta tttgtaaagt tttgttgaaa taagggttgt aattgtgatc
     2401 acgcccgcac ataacccaca gggtgttgtc tatactttac acataaggaa gaggggtatt
     2461 ccctgttaca acccagaaag ttccggaggt gacatatgaa acgcaaaaac gcttcgttac
     2521 tcggtaacgt gctcatgggg ttggggctgg tggtaatggt ggtcggcgtg gggtattcaa
     2581 tcctcaacca gttaccacag tttaatatgc cccagtattt cgcacatggt gcagtgctaa
     2641 gtattttcgt cggtgccatt ctctggctgg cgggtgcccg tgttggcggg catgaacagg
     2701 tgtgcgaccg ttactggtgg gttcgtcact atgacaaacg ttgccgccgt agcgataatc
     2761 gccgtcatag ctaacaaata atgcagtttg ctgaccagtc agtttgcgct gactggtcaa
     2821 tctcgtactt ataaatccgc catcgccgcg cgacgattcg ggaagaacgc gaggcgtccc
     2881 gggatcggtt gaatgcctgc gcgagccata gtgcgcagtg gctggaattc cacgttgcac
     2941 acgcgcagtt cacatccttc gggcagacgc ttcacaaaac gctggaacgc atcaagacca
     3001 ccagcatcaa gtaccggaac ggcatcccac ttcagaatca caatccgttt gccttcaaga
     3061 cgtgactcca ggtccgtgaa caagccttca gcagcagcaa aaaacagcgg gccaataacg
     3121 cgcagtacca gaacatcgtc tggaacatct acgactaccg gtgccaggcg agtcatacgt
     3181 gcgatacgac gcataaacag cagcgatgcc agcacgatcc ccacgctgat ggcaataacc
     3241 atatcaaaca gcacggtcag cgacatgcac agcagcatga caatgatgtc atctttcggt
     3301 gcatgacgca gcaagtcgac cactttatgc gcttcactca tgttccacgc caccatcaac
     3361 agcagggctg ccatagcgga aagcggcagc caggagagca gcggtgccag taccagcagg
     3421 gcaagaataa ccagaataga gtggatcacc gccgagatag gggaagttgc cccggcacgg
     3481 acgttagcgg cagaacgcgc gatggcagct gtagcggtaa taccaccaaa gaacggagcg
     3541 ataatattcc ccagtccctg tccaaccagt tcgctgttcg ccttgtgttt cgtcccggtc
     3601 ataccatcca gtaccacggc gcagagcaga gattcgattg cgccgagcat tgccattgag
     3661 aatgccgcag gcagcagtgt gcgaatagaa tcccaggtta gcgtgaattc tgaattaggc
     3721 agatcccacg gcagcaccag ttgcggcagc agttgcggaa taccgttacc ctgagaacca
     3781 tcggccagga cgtagtggaa ttgcgaaccg atggtagcaa catgtccgcc gagcaggtta
     3841 acaatcccca tcaccgcgca accagccagc aaggccggaa ggtgaccggg taaacgaatg
     3901 cccagacgcg gccaaaaaac aagaatacct agcgtcacaa tgccaatggc agcatcaccc
     3961 acattaatgg tcggcagcgc cataaataat gcgccgactt tttgtagata atgttccggg
     4021 acatgggcca tttgcagacc gagaaaatct ttaatctgca tggtaccgat ggtgatcccg
     4081 atacccgagg tgaaacctaa ggtgacggaa accggaatat actcaatcag gcgaccaaag
     4141 cgtgccagac ccatcagaat caaaaagatc cccgacagca aggtcgcaac cagcagtcct
     4201 gccagtccaa actgttgcga cacgggatag agaattacca caaatgccgc agtcggaccg
     4261 gaaacgctaa agcgtgaccc acccgtcaga gcaatgacaa tccccgcaac agctgcggta
     4321 tataaaccgt actggggtgc cacaccacta ccaatagcca acgccatcgc cagcgggata
     4381 gcaataatcc cgacggttat cccggcaatc aggtcacggg taaaccgtgc ggcagtatat
     4441 ttttctttcc agcaagcgtc gatcagagcg cggaaaggca tcacatgtga ggaaaatatt
     4501 ttgttcacaa taatgtttca tccgtgagcg catcatctgt caactaaatg gcaggtgaag
     4561 gaggcatagg tcatacaaat ggatattaca gacaaaaaaa cccgccgcag cgggtctttg
     4621 agcgggttcg attagtgttc gaacatggca gagatcgatt cttcgttgct gatacgacga
     4681 atcgcttcgg ccagcatacc tgacagggtc agagtacgca cgttcggcag tgatttgatt
     4741 tcatcgctca gcggaatggt atcgcagaca acgacttcat caattacaga gttacgcagg
     4801 ttgttcgccg cgttgccaga gaagatcggg tgagtcgcgt acgcaaatac acgtttagca
     4861 ccacgttctt tcagagcttc agcagcttta cacagcgtac cgccagtgtc gatcatatca
     4921 tcgaccagta cgcagtcacg acctgcaacg tcaccgatga tatgcatcac ctgggaaacg
     4981 ttcgcacgcg gacgacgttt gtcgatgatt gccatatcgg tatcgttcag cagcttagcg
     5041 atagcgcggg cacgcacaac gccgccgatg tccggagaaa ccacaattgg gttatccaga
     5101 ttcagctgca gcatgtcttc cagcaggatc gggctaccaa atacgttatc aaccggaacg
     5161 tcgaagaaac cctgaatctg ttcagcgtgc agatccactg tcagcacacg gtcaacaccg
     5221 acgctggaga ggaagtctgc aaccactttc gcagtgattg gtacacgagc ggaacggacg
     5281 cgacggtcct ggcgcgcata gccaaagtag gggataacag cggtgatacg acctgcggaa
     5341 gcacgacgca gggcatcaac cataacgact aattccatca ggttgtcgtt agtaggggca
     5401 caagtggact ggatgatgaa aatatcacca ccgcgtacat tttcgttgat ttgtacactg
     5461 acttcgccgt cgctaaagcg acctacagcg gcgtcgccga gtgaagtgta caggcggttg
     5521 gcaatacgtt gtgctagttc cggggtggcg ttaccagcaa aaagcttcat atcaggcacg
     5581 agaagaacct caggcatgcg tccattggtg gaaagaatct gccgaaaact gtgcgggcca
     5641 ggcatgatcc tttccaggcg gtgtattaaa gagcgcgatg caacgtctgg aacaaggtga
     5701 cgttgtcacc gaaactcagc ttgcccggct taaagcatgg ctctgtgcaa tggggaaaga
     5761 ttaacacctt tcgccacaaa gccattgagc cattccgggg cttgctctag cacctggcgg
     5821 gcttcagact ctgtatcaaa ttcagcaaag acacaggccc ctgtcccagt caggcgcgac
     5881 ggggcgtatt ctaacagcca ggaaagcacc gcatcaacct cgcgaaaacg ttttcttgcg
     5941 ataacctcgc aatcattgct gaattcacat tttagcaacg tttctattga cctttttggc
     6001 gtattgcgcg ggagttcagg atctttaaaa atcactggtg tcggaatact tacaccaggg
     6061 tgcgccacca gataccactt ctctggcgga tccaccggcg ttagtatttc accaacgcct
     6121 tcggcaaacg cggcatgccc ccgaacaaag acaggaacat ctgcgcccag cgtcagcccc
     6181 atttccgcca gctcatccat gcttagcccg cattgccaga gatgatttaa tgccaccagg
     6241 accgtcgcgg cattggatga accaccgccg agaccgccgc ccatcggcaa acgcttgtca
     6301 atgctgatat tcgcaccgct tcccgtcgga agacgcccgc tgtctgccgc agttttcatc
     6361 aacaaccgcg ctgcgcgaac gatcaggtta tcttcatgtt ccacgccttc aacgggcgtt
     6421 aacagacgaa tatccccatc gtcacgaagc tcaatgctga tggtgtcgcc gtaatcaaga
     6481 aactgaaaca gcgtttgcag cgtgtggtaa ccatccgcac gctgaccggt aatgtataaa
     6541 aacagattaa gttttgccgg agagggccac tgtgtccgca ttatttcact atccagttat
     6601 ccatttttaa cttgatgcgt tgaccaccgt cggtgagttc catattggct ggcatcgcag
     6661 gttgcgtttt ggtgtcataa ccaccataaa caaccttcca gtttttgcca ttctggctgt
     6721 aggtaatttc gctcaggcgg tactggtcgt ccagtttgta gtcggttgca tcacccggta
     6781 aacctaaaat ccattggcgc aagctgttga gcggaattgg cattccggtc aatttgccaa
     6841 tcatctcttc ggcgtcatcg gcggtataac gctgaccttt attgtcgact aactgcacgt
     6901 tacccggttg agcattcagc tccagttccg tgctgcccaa tgggttagtg agcagcagac
     6961 ggtagcgatc ctggccggtt tgctgccaga aaaagcgggc gtacactttt tgttggtcag
     7021 aaatataagc gaacgcgccg cgagtctgat actgattaag attgcgcacg tcttgctgat
     7081 gctgacgcca ttgtggcgaa tccgggcttt tgccaggacc tttgggcgtg gtaacggaac
     7141 aggcagtgag cacaagagca gccagcggta gcaggcggat aagacgaaaa tcgggctggg
     7201 gcatagtgat gacaagtcct tgagatacgt tgcagttata acccttaatg ctagcgttac
     7261 cgtccgctat cgtctatgtt caagttgtct taattgccag aatctaacgg ctttcggcaa
     7321 ttactccaaa agggggcgct ctcttttatt gatcttacgc atcctgtatg atgcaagcag
     7381 actaaccata tcaacgttgg tattatttcc cgcagacatg acccttttag cactcggtat
     7441 caaccataaa acggcacctg tatcgctgcg agaacgtgta tcgttttcgc cggataagct
     7501 cgatcaggcg cttgacagcc tgcttgcgca gccgatggtg cagggcggcg tggtgctgtc
     7561 gacgtgcaac cgcacggaac tttatcttag cgttgaagag caggataacc tgcaagaggc
     7621 gttaatccgc tggctttgcg attatcacaa tcttaatgaa gaagatctgc gtaaaagcct
     7681 ctactggcat caggataacg acgcggttag ccatttaatg cgtgttgcca gcggcctgga
     7741 ttcactggtt cttggggagc cgcagatcct cggtcaggtt aaaaaagcgt ttgccgattc
     7801 gcaaaaaggt catatgaagg ccagcgaact ggaacgcatg ttccagaaat ctttctctgt
     7861 agcgaaacgc gttcgcactg aaacagatat cggtgccagt gctgtgtctg tcgcttttgc
     7921 ggcttgtacg ctggcgcggc agatctttga atcgctctct acggtcacag tgttgctggt
     7981 aggcgcgggc gaaaccatcg agctggtagc gcgtcatctg cgcgaacata aagtacagaa
     8041 gatgattatc gccaaccgca ctcgcgaacg tgcccaaatt ctggcagatg aagttggcgc
     8101 ggaagtgatt gccctgagtg agatcgacga acgtctgcgc gaagccgata tcatcatcag
     8161 ttccaccgcc agcccgttac cgattatcgg gaaaggcatg gtggagcgcg cattaaaaag
     8221 tcgtcgtaac caaccaatgc tgttggtgga tattgccgtt ccgcgcgatg ttgagccgga
     8281 agttggcaaa ctggcgaatg cttatcttta tagtgtggac gatctgcaaa gcatcatttc
     8341 gcacaacctg gcgcagcgta aagccgcagc ggttgaggcg gaaactattg tcgctcagga
     8401 aaccagcgaa tttatggcgt ggctgcgagc acaaagcgcc agcgaaacca ttcgcgagta
     8461 tcgcagccag gcagagcacg ttcgcgatga gttaaccgcc aaagcgttag cggcccttga
     8521 gcagggcggc gacgcgcaag ccattatgca ggatctggca tggaaactga ctaaccgctt
     8581 gatccatgcg ccaacgaaat cacttcaaca ggccgcccgt gacggggata acgaacgcct
     8641 gaatattctg cgcgacagcc tcgggctgga gtagcagtac atcattttct ttttttacag
     8701 ggtgcattta cgcctatgaa gccttctatc gttgccaaac tggaagccct gcatgaacgc
     8761 catgaagaag ttcaggcgtt gctgggtgac gcgcaaacta tcgccgacca ggaacgtttt
     8821 cgcgcattat cacgcgaata tgcgcagtta agtgatgttt cgcgctgttt taccgactgg
     8881 caacaggttc aggaagatat cgaaaccgca cagatgatgc tcgatgatcc tgaaatgcgt
     8941 gagatggcgc aggatgaact gcgcgaagct aaagaaaaaa gcgagcaact ggaacagcaa
     9001 ttacaggttc tgttactgcc aaaagatcct gatgacgaac gtaacgcctt cctcgaagtc
     9061 cgagccggaa ccggcggcga cgaagcggcg ctgttcgcgg gcgatctgtt ccgtatgtac
     9121 agccgttatg ccgaagcccg ccgctggcgg gtagaaatca tgagcgccag cgagggtgaa
     9181 catggtggtt ataaagagat catcgccaaa attagcggtg atggtgtgta tggtcgtctg
     9241 aaatttgaat ccggcggtca tcgcgtgcag cgtgttcctg ctacggaatc gcagggtcgt
     9301 attcatactt ctgcttgtac cgttgcggta atgccagaac tgcctgacgc agaactgccg
     9361 gacatcaacc cagcagattt acgcattgat actttccgct cgtcaggggc gggtggtcag
     9421 cacgttaaca ccaccgattc ggcaattcgt attactcact tgccgaccgg gattgttgtt
     9481 gaatgtcagg acgaacgttc acaacataaa aacaaagcta aagcactttc tgtacttggt
     9541 gctcgtatcc acgctgcaga aatggcaaaa cggcaacagg ccgaagcgtc tacccgtcgt
     9601 aacctgctgg ggagtggcga tcgcagcgac cgtaaccgta cttacaactt cccgcagggg
     9661 cgcgttaccg atcaccgcat caacctgacg ctctaccgtc tggatgaagt gatggaaggt
     9721 aagctggata tgctgattga accgattatc caggaacatc aggccgacca actggcggcg
     9781 ttgtccgagc aggaataatg gaatatcaat actggttacg tgaagcaata agccaacttc
     9841 aggcgagcga aagcccacgc cgtgacgctg aaatcttatt ggagtatgtc actggcaggg
     9901 ggcggacttt tattctggcc tttggtgaaa cgcagctgac tgacgaacaa tgtcagcaac
     9961 ttgatgcgct actgacacgt cgtcgcgatg gtgaacccat tgctcattta accggggtgc
    10021 gagaattctg gtcgttgccg ttatttgttt cgccagcgac cttaattccg cgcccggata
    10081 cggagtgtct ggtggagcag gcactggcgc ggttgcctga acaaccttgc cgtattctcg
    10141 atctcgggac gggtaccggg gcgattgcgc ttgcgctggc tagcgagcgc ccggactgcg
    10201 aaattatcgc tgtagatcgt atgcctgatg ctgtctccct ggcacaacgt aatgcccagc
    10261 atctggcgat caaaaatatc cacattctgc aaagcgactg gtttagcgcg ctagccgggc
    10321 agcagtttgc gatgattgtc agcaatccgc cgtatattga cgagcaggac ccacatcttc
    10381 aacaaggcga tgtccgcttt gagccgctca ctgcgctggt tgcggcagac agtggaatgg
    10441 cagacatcgt gcatatcatc gaacagtcgc gtaacgcgct ggtatccggc ggctttctgc
    10501 ttctggaaca tggctggcag cagggcgaag cggtgcgaca agcatttatc ctcgcggggt
    10561 atcatgacgt cgaaacctgc cgtgactatg gtgataacga gcgcgtaacg ctcggccgct
    10621 attatcaatg acaagttttt ctacactgct tagtgttcat cttattagta tcgcgctttc
    10681 tgttgggcta ttaaccttac gtttctggct acgttatcag aagcatccac aggcatttgc
    10741 tcgctggacg cgcattgtgc cgccggttgt cgatacggtg ttattgttaa gcggcattgc
    10801 gttgatggct aaagcgcaca tcctgccatt ttccgggcag gcacagtggc tgactgaaaa
    10861 gctgtttgga gttatcattt atatcgtttt gggttttatt gcactcgatt atcgtcgtat
    10921 gcacagtcag caggcgcgca ttattgcctt cccgctggcg ttggtggtgc tgtacatcat
    10981 cattaaactc gccaccacaa aagtaccgtt actggggtaa gtcatgagat cgttagctga
    11041 tttcgaattt aataaagcgc cattgtgcga aggcatgatc ctggcttgcg aagcaatccg
    11101 ccgcgatttt ccctcgcaag atgtttacga cgaactggag cgtctcgtta gtctggcgaa
    11161 ggaagaaatc agccagcttc tgcctttaga agagcagctg gaaaaactga tcgcgctgtt
    11221 ttacggcgac tggggattta aagcctcacg cggcgtttat cgtctttccg atgcattatg
    11281 gctggaccag gtgttaaaga atcgacaggc agtgcggtat cattaggtgc ggttttatta
    11341 tgggtcgcga atcgtctcga tttgccgctg ctgccggtga ttttccctac gcagctgata
    11401 ttgcgcattg aatgtccgga tggcgaaatt tggctgatta atccttttaa cggtgaatcg
    11461 ttaagcgaac atatgctgga cgtatggtta aagggaaata tcagcccgtc ggcggaactg
    11521 ttttatgaag accttgatga agctgataac attgaggtaa tccgcaaatt gttggataca
    11581 ctcaaagcct cgttgatgga agaaaatcag atggagctgg cgttacgcac cagcgaagct
    11641 ttattacaat tcaacccgga agatccctat gaaattcgcg atcgcgggtt gatttatgcg
    11701 caactggatt gcgaacacgt tgcgttgaac gatttaagtt atttcgttga acagtgtccg
    11761 gaagacccga tcagcgaaat gatccgtgcg caaataaata acatcgcgca taaacatatt
    11821 gtgctgcatt aattaatcga cattttactc aagattaagg cgatcctatg aaacaaaaag
    11881 tggttagcat tggcgacatc aacgtagcaa atgacctgcc gttcgtactg tttggtggta
    11941 tgaacgtgct ggaatctcgc gatctggcga tgcgcatttg cgagcactac gtaaccgtga
    12001 cccagaaact gggtatccct tacgtgttca aagcctcttt tgacaaagcc aaccgctcct
    12061 ccatccactc ttatcgtgga ccgggcctgg aagaagggat gaaaatcttc caggagttga
    12121 agcagacttt tggcgtgaaa attatcaccg acgttcacga accaagtcag gcacagcccg
    12181 ttgctgatgt cgtggatgtg attcagttgc cggcgtttct tgctcgccag actgacctgg
    12241 ttgaagccat ggcgaaaacc ggtgcggtaa ttaacgtcaa gaaaccacag tttgtcagcc
    12301 cgggacagat gggtaatatc gttgataaat tcaaagaagg cggcaacgaa aaagtgattc
    12361 tttgcgatcg cggtgctaac ttcggctatg acaacctggt tgtcgatatg ctgggcttca
    12421 gcattatgaa gaaagtgtct ggtaactcgc cggtgatttt cgacgtgacc cacgcactgc
    12481 aatgccgcga tccgtttggc gcagcttccg gtggtcgtcg tgctcaggtg gctgagctgg
    12541 cacgagccgg tatggcggta ggtctggcgg ggctgtttat tgaagcgcat ccggatccgg
    12601 aacatgcgaa atgtgatggt ccatccgcgc tgccgctggc taaactggaa ccgttcctca
    12661 agcagatgaa agcgattgat gatctggtga aaggtttcga agaactggat accagcaagt
    12721 aatctttttt gcttgaaaaa taaagcctta gcgttctgcg ttaagacttt tttcatgggt
    12781 gccagataca aaaa
//
LOCUS       DAJTYZ010000113        12409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000113 DAJTYZ010000000
VERSION     DAJTYZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.113"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO596_003126"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO596_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6718918.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO596_003127"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO596_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6718919.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="OO596_003128"
     CDS             complement(2659..5772)
                     /locus_tag="OO596_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6718920.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OO596_003129"
     CDS             complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OO596_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR6718921.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="OO596_003130"
     CDS             7014..7292
                     /locus_tag="OO596_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6718922.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OO596_003131"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OO596_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6718923.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OO596_003132"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OO596_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6718924.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="OO596_003133"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="OO596_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6718925.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="OO596_003134"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="OO596_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6718926.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /locus_tag="OO596_003135"
     CDS             10284..10931
                     /locus_tag="OO596_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR6718927.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10973..11503)
                     /locus_tag="OO596_003136"
     CDS             complement(10973..11503)
                     /locus_tag="OO596_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6718928.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11659..12225)
                     /gene="slp"
                     /locus_tag="OO596_003137"
     CDS             complement(11659..12225)
                     /gene="slp"
                     /locus_tag="OO596_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6718929.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3430 a         2753 c         2808 g         3418 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct cataaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcga gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgagtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagt gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggtttt ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata cctggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttacccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactccgt agcggagaga gtattaatcg gatcatagtc
     8581 atatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gtagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gtagtcgatt taataaaaat ttcctaatta cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tatatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtctgac gacggcagcg gatatctggg tgaccgccgc cataggtatg gttattggca
    10621 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
    10681 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatat ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa actgatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
    10861 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
    10981 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
    11041 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
    11101 cttcagttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
    11161 acacattcgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
    11221 tagtgatagt ccgcggaaca aaggtcaccg gtactttccc cagacagcgt ttaatattaa
    11281 agggggagaa aacaatgaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
    11341 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
    11401 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
    11461 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
    11521 gacattaact ttataatcaa tttgccaaag gtaattaaca ggacagggct tataataata
    11581 atagttagct tactgtatat taattttatg taatacatga tgcagatgct gagaaagcat
    11641 ctgcatcttt cggtggtgtt atttgaccag ctcaggtgtt acctgactca ccgcattggt
    11701 gtagtaaggc gcaccccagc ccggttccgg ccagaatgca ccatagccgt aatcccacag
    11761 gttataggtg gtattaacca cttctctcaa atgccacacc tggatgccct gcatattcac
    11821 ttccaggaag ttatacggga ctttattgat aaagccaggt tgttcaccct gaatggtacc
    11881 gaggatggta acaaagtgat tacgatagtt cactgggtca aggaagccgc tttgtctggc
    11941 gagcagtcgg ccctgatagt tggcttcaat atcaggtttc gcatagctat ccaacggtaa
    12001 tacagcgatt tctaacaacg tatccgtttt gccattgata acgttgataa ccttcccacc
    12061 aaagcgcgct tgttgaccaa catataaccc cggctggtta tgaacagcaa caaaactttt
    12121 ttgaatatca ggttggttat tgcctttgat attttgcgga attgaactac atgcggcaag
    12181 caaaaatgaa aggctgagga tgagtgcacc ttttgtcatg ttcatgttac tatccttatc
    12241 aacaaatatt aaaaccataa tagatatttc tttatagttt tcatctgatt ctgaggcgat
    12301 atgcctgata gtaacgtaaa cctgtctgat gtttatatcc cttttgttta gttcaattcc
    12361 attgattttg gggcgtggca tgagtatcca tcgccccata atataatcc
//
LOCUS       DAJTYZ010000114        12186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000114 DAJTYZ010000000
VERSION     DAJTYZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12186
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.114"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="OO596_003138"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="OO596_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6718930.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="OO596_003139"
     CDS             433..621
                     /locus_tag="OO596_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6718931.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="OO596_003140"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="OO596_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR6718932.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="OO596_003141"
     CDS             816..989
                     /locus_tag="OO596_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6718933.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="OO596_003142"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="OO596_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OO596_003143"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OO596_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCR6718934.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="OO596_003144"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="OO596_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6718935.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OO596_003145"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OO596_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6718936.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="OO596_003146"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="OO596_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6718937.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="OO596_003147"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="OO596_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OO596_003148"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OO596_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6718938.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..>12186)
                     /gene="cbpA"
                     /locus_tag="OO596_003149"
     CDS             complement(11350..>12186)
                     /gene="cbpA"
                     /locus_tag="OO596_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6718939.1"
                     /translation="ARKYHPDVSKEPDAEARFKEVAEAWEVLSDEQRRAEYDQMWQHR
                     NDPQFNHQFHHGDGQSFNAEDFDDIFSSIFGQHARQSRQRPAARGHDIEIEVAVFLEE
                     TLTEHKRTISYNLPVYNAFGMIEQEIPKTLNVKIPAGVGNGQRIRLKGQGTPGENGGP
                     NGDLWLVIHIAPHPLFDIVGQDLEIVVPVSPWEAALGAKVTVPTLKESILLTIPPGSQ
                     AGQRLRVKGKGLVSKKQTGDLYAVLKIVMPPKPDENTAALWQQLADAQSSFDPRKDWG
                     KA"
BASE COUNT         2921 a         3146 c         3200 g         2919 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggtat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggc
//
LOCUS       DAJTYZ010000115        12036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000115 DAJTYZ010000000
VERSION     DAJTYZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12036
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.115"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..430
                     /locus_tag="OO596_003150"
     CDS             <1..430
                     /locus_tag="OO596_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HCR6718940.1"
                     /translation="AMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTI
                     RLPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESI
                     SIERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(476..1069)
                     /gene="yhgN"
                     /locus_tag="OO596_003151"
     CDS             complement(476..1069)
                     /gene="yhgN"
                     /locus_tag="OO596_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6718941.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            1261..2364
                     /gene="asd"
                     /locus_tag="OO596_003152"
     CDS             1261..2364
                     /gene="asd"
                     /locus_tag="OO596_003152"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6718942.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            2637..4823
                     /gene="glgB"
                     /locus_tag="OO596_003153"
     CDS             2637..4823
                     /gene="glgB"
                     /locus_tag="OO596_003153"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6718943.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            4820..6793
                     /gene="glgX"
                     /locus_tag="OO596_003154"
     CDS             4820..6793
                     /gene="glgX"
                     /locus_tag="OO596_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6718944.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            6811..8106
                     /gene="glgC"
                     /locus_tag="OO596_003155"
     CDS             6811..8106
                     /gene="glgC"
                     /locus_tag="OO596_003155"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6718945.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            8106..9539
                     /gene="glgA"
                     /locus_tag="OO596_003156"
     CDS             8106..9539
                     /gene="glgA"
                     /locus_tag="OO596_003156"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6718946.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            9558..12005
                     /gene="glgP"
                     /locus_tag="OO596_003157"
     CDS             9558..12005
                     /gene="glgP"
                     /locus_tag="OO596_003157"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6718947.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         2828 a         3005 c         3301 g         2902 t
ORIGIN      
        1 ggcgatggag gcattaaaac atcagtggct gcctgtgcag gttaaaccaa atcaggatat
       61 gcaatcagcc aattcacgat tagtagaaaa tggggcgtca tggagtgctg aacaggctga
      121 gaatgtgacg atcagactgc cagacgttcc tgggctgatg tatgacagag cactccgtaa
      181 cgctcaacca gaactgtttt cgctgcatga agatgacgca aattacgcag taatgcccgc
      241 gtatacgcct gtcgattttt atcaactctt tgtggcggaa ctggcgatcc ttgcaaagga
      301 atcgataagt attgaaaggc tggcgtcttg tactggttta accatcgaac aaattagtgt
      361 gtggctgaac cgcgcagaag aagagggaag ggttatccga ttgggcgaag gtcattatca
      421 gttcaggtaa taaaacaggt gccgcgtctg gttgtgtgca gcacctgttt gaaccttacc
      481 ccttcatcca cattcgaatg ccgtcgagga acatctgggt tgccatcatc accagaatca
      541 atcccatcag gcgttcaagt gcgttcaccc ctttctcgcc cagcagacgt aaaaatagcg
      601 aagactgtag caggatgaca aaggtgccgc cccaggccag cagcagagca atcaccagat
      661 gtcccatctg attcgggtac tgatgagaca acaacatcag cgtggcgaga atagtcggcc
      721 cggcgactaa cggaattgcc aacggcacga taaatggctc ttcacctgcc ggaagcccgc
      781 tgctatttcc tgaagcgctg gggaaaatca tcttaatggc gattagaaac agaatgatgc
      841 cgccagaaat ggagacggtt tctgctcgca ggctgagaaa tgccagaatt ttctcacccg
      901 caaacaggaa caccagcatc accaggagag caataagcaa ctctcgcacc atgattgccc
      961 gccgtctttt cggttcagta tgtttcagta cggacatgaa aataggcagg tttccgagcg
     1021 gatccataat caggatcaat aaaactgctg cagaaatgat tgcattcata actcaaattc
     1081 cctgataatt gccgcggact ttctgcgtgc taacaaagca ggataagtcg cattactgat
     1141 ggcttcgcta tcattgatta atttcacttg cgactttggc tgctttttgt atggtgaaag
     1201 atgtgccaag aggagaccgg cacatttata cagcacacat ctttgcagga aaaaacgctt
     1261 atgaaaaatg ttggttttat cggctggcgc ggtatggtcg gctccgttct catgcaacgc
     1321 atggttgaag agcgcgactt cgacgccatt cgccctgtct tcttttctac ttctcagctt
     1381 ggccaggctg cgccgtcttt tggcggaacc actggcacac ttcaggatgc ctttgatctg
     1441 gaggcgctaa aggccctcga tatcattgtg acctgtcagg gcggcgatta taccaacgaa
     1501 atctatccaa agcttcgtga aagcggatgg caaggttact ggattgacgc agcatcgtct
     1561 ctgcgcatga aagatgacgc catcatcatt cttgaccccg tcaatcagga cgtcattacc
     1621 gacggattaa ataatggcat caggactttt gttggcggta actgtaccgt aagcctgatg
     1681 ttgatgtcgt tgggtggttt attcgccaat gatcttgttg attgggtgtc cgttgcaacc
     1741 taccaggccg cttccggcgg tggtgcgcga catatgcgtg agttattaac ccagatgggc
     1801 catctgtatg gccatgtggc agatgaactc gcgaccccgt cctctgctat tctcgatatc
     1861 gaacgcaaag tcacaacctt aacccgtagc ggtgagctgc cggtggataa ctttggcgtg
     1921 ccgctggcgg gtagcctgat tccgtggatc gacaaacagc tcgataacgg tcagagccgc
     1981 gaagagtgga aagggcaggc ggaaaccaac aagatcctca acacatcttc cgtaattccg
     2041 gtagatggtt tatgtgtgcg tgtcggggca ttgcgctgcc acagccaggc attcactatt
     2101 aaattgaaaa aagatgtgtc tattccgacc gtcgaagaac tgctggctgc gcacaatccg
     2161 tgggcgaaag tcgttccgaa cgatcgggaa atcactatgc gtgagctaac cccagctgcc
     2221 gttaccggca cgctgaccac gccggtaggc cgcctgcgta agctgaatat gggaccagag
     2281 ttcctgtcag cctttaccgt gggcgaccag ctgctgtggg gggccgcgga gccgctgcgt
     2341 cggatgcttc gtcaactggc gtaatcttta ttcattaaat ctggggcgcg atgccgcccc
     2401 tgttagtgcg taatacagga gtaagcgcag atgtttcatg atttaccggg agttaaatag
     2461 agcattggct attctttaag ggtggctgaa tacatgagta ttcacagcct tacctgaagt
     2521 gaggacgacg cagagaggat gcacagagtg ctgcgccgtt caggtcaaaa aaatgtcaca
     2581 accagaagtc aaaaatccaa ttggatgggg tgacacaata aaacaggaag acaagcatgt
     2641 ccgatcgtat cgatagagac gtgattaacg cgctaattgc aggccatttt gcggatcctt
     2701 tttccgtact gggaatgcat aaaaccaccg cgggactgga agtccgtgcc cttttacccg
     2761 acgctaccga tgtgtgggtg attgaaccga aaaccgggcg caaactcgca aaactggagt
     2821 gtctcgactc acggggattc tttagcggcg tcattccgcg acgtaagaat tttttccgct
     2881 atcagttggc tgttgtctgg catggtcagc aaaacctgat tgatgatcct taccgttttg
     2941 gtccgctaat ccaggaaatg gatgcctggc tattatctga aggtactcac ctgcgcccgt
     3001 atgaaacctt aggcgcgcat gcagatacta tggatggcgt cacaggtacg cgtttttctg
     3061 tctgggctcc aaacgcccgt cgggtctcgg tggttgggca attcaactac tgggacggtc
     3121 gccgtcaccc gatgcgcctg cgtaaagaga gcggcatttg ggaactgttt atccctgggg
     3181 cgcataacgg tcagctctat aaatacgaga tgattgatgc caatggcaac ttgcgtctga
     3241 agtccgaccc ttatgccttc gaagcgcaaa tgcgcccgga aaccgcgtct cttatttgcg
     3301 ggctgccgga aaaggttgta cagactgaag agcgcaaaaa agcgaatcag tttgatgcgc
     3361 caatctctat ttatgaagtt cacctgggct cctggcgtcg ccacaccgat aacaatttct
     3421 ggttgagcta ccgcgagctg gccgatcaac tggtgcctta tgctaaatgg atgggcttta
     3481 cccacctcga actactgccc attaacgagc atcccttcga tggcagttgg ggttatcagc
     3541 caaccggcct gtatgcacca acccgccgtt ttggtactcg cgacgacttc cgttatttca
     3601 ttgatgccgc acacgcagcg ggtctgaacg tgattctcga ctgggtgcca ggccacttcc
     3661 cgaccgatga ctttgcgctt gccgaatttg atggcacgaa cttgtatgaa cacagcgatc
     3721 cgcgcgaagg ctatcatcag gactggaata cgctgatcta caactatggt cgccgtgaag
     3781 tcagtaactt cctcgtcggt aacgcgcttt actggattga acgttttggt attgatgcgc
     3841 tgcgcgtcga tgcggtggcg tcaatgattt atcgcgacta cagccgtaaa gagggggagt
     3901 ggatcccgaa cgaatttggc gggcgcgaga atcttgaagc gattgaattc ttgcgtaata
     3961 ccaaccgtat tcttggtgag caggtttccg gtgcggtgac aatggctgag gagtctaccg
     4021 atttccctgg cgtttctcgt ccgcaggata tgggcggtct gggcttctgg tacaagtgga
     4081 acctcggctg gatgcatgac accctggact acatgaagct cgacccggtt tatcgtcagt
     4141 atcatcacga taaactcacc ttcgggattc tctacaacta cactgaaaac ttcgtcctgc
     4201 cgttgtcgca tgatgaagtg gtccacggta aaaaatcgat tctcgaccgc atgccgggcg
     4261 acgcatggca gaaattcgct aacctgcgcg cctactatgg ctggatgtgg gcattcccgg
     4321 gcaagaaact gctgttcatg gggaacgaat ttgcccaagg ccgtgagtgg aaccatgacg
     4381 ccagcctcga ctggcatctg ttggaaggcg gcgataactg gcaccacggt gtccagcgtc
     4441 tggtgcgcga tctgaacctc acctaccgcc accataaagc aatgcatgaa ctggattttg
     4501 acccgtacgg ctttgaatgg ctagtggtgg atgacaaaga acgctcggtg ctgatctttg
     4561 tgcgtcgcga taaagagggt aacgaaatta tcgttgccag taactttacg ccggtgccgc
     4621 gtcatgatta tcgcttcggc attaatcagc caggcaaatg gcgtgaaatc ctcaataccg
     4681 attccatgca ctatcacggc agtaatgcag gcaatggcgg cacggtacac agcgatgaga
     4741 ttgccagcca cggtcgtcag cattcactaa gcctgacact accaccgctg gccactatct
     4801 ggctggttcg ggaggcagaa tgacacgact cgccattggc aaacccgctc ccctcggcgc
     4861 gcattacgac ggtcagggcg tcaacttcac acttttctcc gctcatgccg agcgggtaga
     4921 gctgtgtgtc tttgacgcca atggccagga acatcgctat gacttgccag ggcacagtgg
     4981 cgacatttgg cacggttatc tgccggatgc gcgcccgggt ttgcgttatg gttatcgcgt
     5041 tcatggcccc tggcaacccg ccgaggggca tcgctttaac ccggcgaagt tgttgattga
     5101 tccttgcgcg cggcaaattg aaggggaatt caaagataac ccgctgctgc acgccggtca
     5161 taatgaacct gactatcgcg acaacgccgc cattgcgccg aaatgcgtag tggtggttga
     5221 tcactatgac tgggaagatg atgccccgcc gcgcacgccg tggggcagca ccatcattta
     5281 tgaagcccat gttaaaggat taacgtacct gcacccggag atcccggtcg agatccgtgg
     5341 cacttataaa gccctcgggc atccggtgat gatcaactat ttgaaacagt tgggcattac
     5401 cgcgctggaa ctgctgccgg tggcgcagtt tgccagtgaa ccacgtctgc aacgcatggg
     5461 gctaagtaac tactggggtt acaacccggt ggcgatgttt gcgctgcatc cggcgtatgc
     5521 ctgctcgcca gaaacggcgc tgcatgagtt tcgcgatgca atcaaagcac tgcataaagc
     5581 gggtatcgaa gtcattcttg atatcgtgct caaccatagt gcggaactgg acctcgacgg
     5641 cccgttattc tcgctgcgtg ggatcgacaa ccgtagctat tattggataa gagaagacgg
     5701 cgattatcac aactggaccg gttgtggcaa cacgctcaat ttgagtcatc cggcggtggt
     5761 ggattatgcc tgcgcctgcc tgcgttattg ggtagaaacc tgtcacgtcg atggtttccg
     5821 ctttgatctg gcggcagtca tgggccgtac gccagagttc cgtcaggatg cgccgttgtt
     5881 taccgctatc cagaactgcc cggtgctctc gcaggtgaag ttaattgctg aaccgtggga
     5941 tatcgccctt ggtggttatc aggtgggaaa tttcccgccg ctgtttgccg agtggaacga
     6001 tcatttccgc gatgctgccc gtcgtttctg gctacattat gatttgcctc tgggggcgtt
     6061 tgccgggcgt tttgctgcct ccagcgatgt ttttaaacgt aatggtcgtc tgccgagtgc
     6121 cgcgattaat ctcgtcactg cgcatgacgg ttttacgctt cgcgactgcg tttgcttcaa
     6181 ccataaacac aatgaagcaa acggagaaga aaatcgcgac gggaccaaca acaattacag
     6241 taacaatcat ggtaaagaag ggttaggcgg ttctcttgac ctggttgaac ggcggcgcga
     6301 cagtattcac gccctgttaa caacgttgtt gctctcccag ggtacgccga tgttactggc
     6361 cggtgacgaa cacggtcaca gccagcatgg caataacaat gcctactgtc aggataacca
     6421 attaacctgg ttggactggt cgcaggcaag cagtggttta accgcattta ccgccgcgtt
     6481 aatccatctg cgcaagcgca ttcccgcttt ggtggagaat cgctggtggg aagaaggcga
     6541 cggcaatgtc cgttggctaa atcgatatgc tcaaccttta agcacggatg agtggcaaaa
     6601 cgggccgaaa cagctgcaaa ttctgctctc ggatcgcttt ttgatcgcaa ttaacgccac
     6661 gcttgaggta acagagattg ttttacctgc tggggagtgg cacgccattc ccccattcgc
     6721 tggagaggat aacccagtga ttacggctgt ctggcaggga cctgcacacg gattgtgtgt
     6781 gttccagaga tgataaaaaa ggagttagtc atggttagtt tagagaagaa cgatcactta
     6841 atgttggcgc gccagctgcc attgaaatct gttgccctga tactggcggg aggacgtggt
     6901 acccgcctga aggatttaac caataagcga gcaaaaccgg ccgtacactt cggcggtaag
     6961 ttccgcatta ttgactttgc gctgtctaac tgcatcaact ctgggatccg tcgtatgggc
     7021 gtgatcaccc agtaccagtc ccacactctg gtgcagcaca ttcagcgcgg ctggtcattc
     7081 ttcaatgaag aaatgaacga gtttgtcgat ctgctgccag cacagcagag aatgaaaggg
     7141 gaaaactggt atcgcggcac cgcagatgcg gtcacccaaa acctcgacat tatccgccgt
     7201 tataaagcgg aatacgtggt gatcctggcg ggcgaccata tctacaagca agactactcg
     7261 cgtatgctta tcgatcacgt cgaaaaaggc gcacgttgca ccgttgcttg tatgccagta
     7321 ccgattgaag aagcctccgc atttggcgtt atggcggttg atgagaacga taaaattatc
     7381 gaattcgttg aaaaacctgc taacccaccg tcaatgccga acgatccgag caaatctctg
     7441 gcgagtatgg gtatctacgt ctttgacgcc gactatctgt atgaactgct ggaagaagac
     7501 gatcgtgatg agaactccag ccacgacttt ggcaaagatt tgattcccaa gatcaccgaa
     7561 gccggtctgg cctatgcgca cccgttcccg ctctcttgcg tacaatccga cccggatgcc
     7621 gagccgtact ggcgcgatgt gggtacgctg gaagcttact ggaaagcgaa cctcgatctg
     7681 gcctctgtgg tgccggaact ggatatgtac gatcgcaatt ggccaattcg cacctacaat
     7741 gaatcattac cgccagcgaa attcgtgcag gatcgctccg gtagccacgg gatgaccctt
     7801 aactcactgg tatccggcgg ctgtgtgatc tccggttcgg tggtggtgca gtccgttctg
     7861 ttctcgcgcg ttcgcgtgaa ttcattctgc aacattgatt ccgccgtatt gttaccggaa
     7921 gtatgggtag gtcgctcgtg ccgtctgcgc cgctgcgtca tcgatcgtgc ttgtgttatt
     7981 ccggaaggca tggtgattgg tgaaaacgca gaggaagatg cacgtcgttt ctatcgttca
     8041 gaagaaggca tcgtgctggt aacgcgcgaa atgctacgga agttagggca taaacaggag
     8101 cgataatgca ggttttacat gtatgttcag agatgttccc gctgcttaaa accggcggtc
     8161 tggctgatgt tattggggca ttacccgcag cacaaatcgc agacggcgtt gacgctcgcg
     8221 tactgttgcc tgcatttccc gatattcgcc gtggcgtgac cgatgcgcag gtagtatccc
     8281 gtcgtgatac cttcgccgga catatcacgc tgttgttcgg tcattacaac ggggttggca
     8341 tatacctgat tgacgcgccg catctctatg atcgtccggg aagcccgtat cacgatacca
     8401 acttatttgc ctataccgac aacgtattgc gttttgcgct tctggggtgg gttggggcag
     8461 aaatggccag cgggcttgac ccattctggc gtcctgatgt ggtgcatgcg cacgactggc
     8521 atgcaggcct tgcgcctgcg tatctggcgg cgcgcgggcg tcctgcgaag tcggtgttta
     8581 ctgtgcacaa cctggcctat caaggcatgt tttatgcaca tcacatgaat gacatccaat
     8641 tgccatggtc attctttaat attcatgggc tggaattcaa cggacaaatc tctttcctga
     8701 aggccggtct gtactatgcc gatcacatta cggcggtcag tccaacctac gctcgcgaga
     8761 tcaccgaacc gcagtttgcc tacggtatgg aaggtctgtt gcaacagcgt catcgtgaag
     8821 ggcgtctttc cggcgtactg aacggcgtgg acgagaaaat ctggagtcca gagacggact
     8881 tactgttggc ctcgcgctac acccgcgata cgttggaaga taaagcggaa aataagcgcc
     8941 agttacaaat cgcaatgggg cttaaggttg acgataaagt gccgcttttt gcagtggtga
     9001 gccgtctgac cagccagaaa ggtctcgacc tggtgctgga agccttaccg ggtcttctgg
     9061 agcagggcgg gcagctggcg ctactcggcg cgggcgatcc ggtgctgcag gaaggtttcc
     9121 ttgcggcggc agcggaatac cccggtcagg tgggcgttca gattggctat cacgaagcat
     9181 tttcgcatcg cattatgggc ggcgcggacg tcattctggt gcccagccgt tttgaaccgt
     9241 gcggcttaac gcaactttat ggattgaagt acggtacgct gccgttagtg cggcgcaccg
     9301 gtgggcttgc tgatacggtt tctgactgtt ctcttgagaa ccttgcagat ggcgtcgcca
     9361 gtgggtttgt ctttgaagat aataatgcct ggtcgctgtt acgggctatt cggcgtgctt
     9421 ttgtactgtg gtcccgtcct tcactgtggc ggtttgtgca acgtcaggct atggcaatgg
     9481 attttagctg gcaggtcgcg gcgaagtcgt accgtgagct ttactatcgc ttgaaatagt
     9541 tttcaggaaa cgcctatatg aatgctccgt ttacatattc atcgcccacg cttagcgtag
     9601 aagctcttaa gcactctatc gcttacaagc tgatgtttac gattggaaag gacccggtcg
     9661 tcgccaataa acatgaatgg ctgaacgcaa cgttatttgc tgtgcgcgat cgtctcgtgg
     9721 agcgctggtt acgttcaaac cgtgcccagt tgtcgcaaga aacacgtcag gtttactacc
     9781 tgtcgatgga gtttttgatt ggccgtacgc tctccaacgc catgttgtcg ctaggaattt
     9841 acgaagatgt acagggcgca ctggaagcga tggggttaaa tctcgaagag ctgattgatg
     9901 aagaaaatga cccaggcctc ggtaacggtg gcctgggacg tctggcggct tgcttccttg
     9961 attctctggc gacgttaggg ttgccggggc gcggttacgg catccgctat gactacggta
    10021 tgttcaagca gaacatcgtt aacggtagcc agaaagagtc gccagactac tggctggaat
    10081 acggtaaccc gtgggaattc aaacgccaca acacgcgcta taaagtccgt tttggcggtc
    10141 gcattcagca ggaaggtaaa aaaacgcgct ggattgaaac cgaagagatt ctgggagtcg
    10201 cttacgatca gataatccct ggttacgaca ccgacgcgac caacacgctg cgtttgtgga
    10261 gtgcgcaagc cagtagcgaa attaacctcg gtaaattcaa ccagggtgac tacttcgcgg
    10321 cagtggaaga taaaaaccac tccgagaacg tatctcgcgt actgtatccg gatgactcca
    10381 cctactccgg gcgtgagctg cgcctgcgtc aggaatactt cctggtttcc tcgaccattc
    10441 aggacatttt aagccgccat tatcagttgc ataaaaccta cgataacctg gcggataaaa
    10501 tcgcgattca tctcaatgat acccatccgg tactgtcgat tcctgagatg atgcgtctgc
    10561 tgatcgatga gcaccaattt agctgggacg acgcgtttga ggtgtgttgt caggtcttct
    10621 cctacactaa ccacacgctg atgagcgagg cgctggaaac ctggccggtt gatatgctgg
    10681 gtaaaattct gccgcgtcac ctgcagatca tctttgaaat caacgactat ttcctgaaaa
    10741 ccttgcagga acagtatccg aacgataccg atctgctggg acgggcgtcg atcattgatg
    10801 aatccaacgg tcgtcgtgtg cgtatggcct ggctggcggt tgttgtgagc cacaaagtta
    10861 acggtgtatc ggaactgcac tctaatctga tggtgcaatc gttgtttgcc gactttgcga
    10921 aaatcttccc gggtcgtttc accaacgtca ccaacggtgt gacgccgcgt cgctggctgg
    10981 cggtagcgaa cccatcgctt tcagccgtgc tggacgaaca cctgggccgt aactggcgca
    11041 ccgaccttag cctgcttaat gagctgcaac aacactgtga tttcccaatg gttaatcacg
    11101 ctgtgcatca ggcgaagctg gagaacaaaa agcgtctggc agagtatatc gcccagcagc
    11161 tgaatgtggt ggtgaatcca aaggcgttgt tcgatgtaca aatcaaacgt attcacgaat
    11221 acaaacgtca attgatgaat gtgttgcatg tgattacccg ctataaccgc atcaaggccg
    11281 acccggatgc gaagtgggta ccgcgcgtga atatttttgg cggtaaggcg gcttcggcct
    11341 attacatggc gaagcacatt attcatttga tcaatgacgt agcgaaagtg atcaacaacg
    11401 atccgcagat tggcgataag ctgaaagtcg tgttcatccc gaactacagc gttagcctgg
    11461 cgcagttgat cattccggcg gcagatctgt ctgaacagat ttcgctggca gggacggaag
    11521 cttccggcac cagtaacatg aagtttgcgc ttaacggtgc gctgactatc ggtacgttgg
    11581 acggtgcgaa tgtcgagatg cgggatcatg tcggtgctga caatatcttt atttttggta
    11641 acacagcgga agaagtggaa gaactgcgtc gtcagggcta caaaccgcgt gaatactacg
    11701 agaaagatga ggagctgcat caggtgctga cgcaaatcgg cagcggtgta ttcagtccgg
    11761 aagatccggg tcgctatcgc gatctggttg attcgctgat caacttcggc gatcactatc
    11821 aggttctggc ggattatcgc agctatgtcg attgtcagga taaagtcgac gaactctacg
    11881 agcttcagga agagtggacc gcaaaagcga tgctgaacat tgccaatatg ggctacttct
    11941 cttctgaccg tactatcaaa gagtacgccg atcatatctg gcatatcgat ccggtgagat
    12001 tgtaagttca ccaataaata gaacggggcc aaaggg
//
LOCUS       DAJTYZ010000116        11915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000116 DAJTYZ010000000
VERSION     DAJTYZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11915
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.116"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..205
                     /locus_tag="OO596_003158"
     CDS             <1..205
                     /locus_tag="OO596_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6718948.1"
                     /translation="STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYN
                     LFKKIAVKNRTQAVSWANDNLRR"
     gene            210..599
                     /gene="csgE"
                     /locus_tag="OO596_003159"
     CDS             210..599
                     /gene="csgE"
                     /locus_tag="OO596_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6718949.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            624..1040
                     /gene="csgF"
                     /locus_tag="OO596_003160"
     CDS             624..1040
                     /gene="csgF"
                     /locus_tag="OO596_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6718950.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1067..1900
                     /gene="csgG"
                     /locus_tag="OO596_003161"
     CDS             1067..1900
                     /gene="csgG"
                     /locus_tag="OO596_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCR6718951.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(1965..2456)
                     /locus_tag="OO596_003162"
     CDS             complement(1965..2456)
                     /locus_tag="OO596_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6718952.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2558..3112)
                     /gene="ycdY"
                     /locus_tag="OO596_003163"
     CDS             complement(2558..3112)
                     /gene="ycdY"
                     /locus_tag="OO596_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6718953.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3136..3873)
                     /gene="ycdX"
                     /locus_tag="OO596_003164"
     CDS             complement(3136..3873)
                     /gene="ycdX"
                     /locus_tag="OO596_003164"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6718954.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3928..4866)
                     /gene="ghrA"
                     /locus_tag="OO596_003165"
     CDS             complement(3928..4866)
                     /gene="ghrA"
                     /locus_tag="OO596_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR6718955.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5100..5187
                     /locus_tag="OO596_003166"
     tRNA            5100..5187
                     /locus_tag="OO596_003166"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5134..5136,aa:Ser,seq:gga)
     gene            5232..5368
                     /locus_tag="OO596_003167"
     ncRNA           5232..5368
                     /ncRNA_class="other"
                     /locus_tag="OO596_003167"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5459..6145)
                     /locus_tag="OO596_003168"
     CDS             complement(5459..6145)
                     /locus_tag="OO596_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6718956.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(6726..7514)
                     /gene="phoH"
                     /locus_tag="OO596_003169"
     CDS             complement(6726..7514)
                     /gene="phoH"
                     /locus_tag="OO596_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6718957.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8135..9406)
                     /gene="efeB"
                     /locus_tag="OO596_003170"
     CDS             complement(8135..9406)
                     /gene="efeB"
                     /locus_tag="OO596_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6718958.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9412..10539)
                     /gene="efeO"
                     /locus_tag="OO596_003171"
     CDS             complement(9412..10539)
                     /gene="efeO"
                     /locus_tag="OO596_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6718959.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10597..11427)
                     /locus_tag="OO596_003172"
     CDS             complement(10597..11427)
                     /locus_tag="OO596_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6718960.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3133 a         3106 c         2761 g         2915 t
ORIGIN      
        1 cagcacggaa tcagccctcc ttactcatcg ggaaaaagag atcctgaata aactgcgtat
       61 cggcgcgtct aataacgaga tcgctcgttc gttgttcatc agcgaaaata cggttaaaac
      121 gcatctttat aatcttttca agaagatagc cgtaaaaaac cggacacaag cggtttcctg
      181 ggcaaacgat aacctcaggc gataaagcca tgaaacgtta tttacgctgg attgtggcgg
      241 cagaatttct gttcgccgca gggaatcttc acgccgttga ggtagaagtc ccgggattgc
      301 taactgacca tactgtttca tctattggcc atgattttta ccgagccttt agtgataaat
      361 gggaaagtga ctatacgggt aacttaacga ttaatgaaag gcccagtgca cgatggggaa
      421 gctggatcac tataacggtc aatcaggacg ttattttcca gactttttta tttccgttga
      481 aaagagactt cgagaaaact gtcgtctttg cactgattca aactgaagaa gcactaaatc
      541 gtcgccagat aaatcaggcg ttattaagta cgggcgattt ggcgcatgat gaattctaaa
      601 taaaaaattg ttcggaggct gcaatgcgtg tcaaacatgc agtagttcta ctcatgctta
      661 tttcgccatt aagttgggct ggaaccatga ctttccagtt ccgtaatcca aactttggtg
      721 gtaacccaaa taatggcgct tttttattaa atagcgctca ggcccaaaac tcttataaag
      781 atccgagcta taacgatgac tttggtattg aaacaccctc agcgttagat aactttactc
      841 aggccatcca gtcacaaatt ttaggtgggc tactgtcgaa tattaatacc ggtaaaccgg
      901 gccgcatggt gaccaacgat tatattgttg atattgctaa ccgcgatggt caattgcagt
      961 tgaacgtgac agatcgtaaa accggacaaa cctcgaccat ccaggtttcg ggtttacaaa
     1021 ataactcaac cgatttttaa gccccagctt cataaggaaa ataatcatgc agcgcttatt
     1081 tcttttggtt gccgtcatgt tactgagcgg atgcttaacc gccccgccta aagaagccgc
     1141 cagaccgaca ttaatgcctc gagctcagag ctacaaagat ttgacccatc tgccagcgcc
     1201 gacgggtaag atctttgttt cggtatacaa cattcaggac gaaaccgggc aatttaaacc
     1261 ctacccggca agtaacttct ccactgctgt tccgcaaagc gccacggcaa tgctggtcac
     1321 ggcattgaaa gattctcgct ggtttatacc gctggagcgt cagagtttac aaaacctgct
     1381 taacgagcgc aagattattc gtgcggcaca agaaaacggc acagttgcca ttaataaccg
     1441 aatcccgctg caatctttaa cggcggcaaa tatcatggtt gaaggttcga ttatcggtta
     1501 tgaaagcaac gttaaatcgg gcggtgttgg ggcaagatat tttgggatcg gtgcagacac
     1561 gcaataccag ctcgatcaga ttgccgtgaa cctgcgtgtc gtcaatgtga gtaccggtga
     1621 gatcctctcc tcggtgaaca ccagtaagac gatactttcc tatgaagttc aggccggggt
     1681 tttccgcttt attgactacc agcgcttgct ggaaggggaa gtgggttaca cctcaaacga
     1741 acccgttatg ctgtgtctga tgtcggctat cgaaacaggg gtcattttcc tgattaatga
     1801 tggtatcgac cgtggtctgt gggatttgca gaacaaagca gaacggcaga atgacattct
     1861 ggttaaatac cgccatatgt cggttccacc agaatcctga cagataaaaa tgccggaatt
     1921 aataatccgg cattttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg
     1981 atttcctgct cacggtgagc cgtctttgca ctacgcgccg ccagccacag gccgctgttt
     2041 ttcattgcgt aaccaaatac cagcccaagc gccagcgaag gtaacaccag tttccagtca
     2101 ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc
     2161 agctgtttgg cctgaataca catcagaaac gccacaatac cggttatgac ataaccgaga
     2221 atttccagat gtggtgccag tgcactaccg taaataatga ccatcgccca caccacgcca
     2281 ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaggcaaag
     2341 taggccgtac agcccaagaa gcccgcccag cttagtaagc ccagggaaac agccacccat
     2401 ccccagatac cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg
     2461 cgcatgatag cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacaaca
     2521 aattatctgt tgcagttatg agtgacgatg atcacattta ctcttcagaa tcttcttcca
     2581 gctcgtccca cattgcacta atggcatcac gggttagcgg tgccatggtg cgccagaaag
     2641 gcgtggttgc atgggcctcc actttgccga ggaacgcacc acaccagggt aacagatact
     2701 cactgaacag tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg
     2761 aagccgcaag cagcaatgtg ccgatgtgat cggctggcgt atccgccagt ggcatccctc
     2821 gttcggaaag aaaagcgcgc acttccgctt ccgttacgtc ctcaacccat gcgctacgat
     2881 atggcggcac tgcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg
     2941 ccatatcaca gcttttctgt aaacgcgtca gtaactcatc ctgctccagt ggccagttcg
     3001 cagccagttt cccctcacga atcagggtaa acagcggcac cagtaaagga tcttgcggtt
     3061 ggcggtaata gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat
     3121 ttccattact agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc
     3181 aaggaagttc agtaagcggc gcggagaaac attcaaaatg cgctctggcg gaaaatctac
     3241 cgcgtcgagg attttaagac actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc
     3301 cgagcctaat gccacccaac ctccagcatc acgtaccgct gcggctaccg cacgacagtt
     3361 gtcttcactg cccttacgtg agtgtaaaaa tgaagaatta ttgatttcca gcgccacctg
     3421 atgtttcgcg gctgcttcag caacggcttt cacatctatt tcatatttgg gatttccggg
     3481 atggcttatt atatgcacat tgccgctggc gatagtggcg atcatcgctt gtgtattggt
     3541 cgctttgtca tgtggtgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga
     3601 gtcaaacatt ttaccgctgc aatcaatttc accatcaaca tttttaatgt tagcttcgat
     3661 gccgcgcagg atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg
     3721 ccagtgatgc ggagcatctt ccatatccgg gccatgatcg gtgatcgcaa aaagtttaat
     3781 gcccttttgt ttggcctggg caatgtaatc actcaatgtg ctatatgcat gtgtgctggc
     3841 aacggtatgc atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc
     3901 aaggatagca ggaatcctga tgctttatta gtagccgcgt gcgcggtcga cttgcccgca
     3961 gaccctctcc cctttttcga gctgggcaat ggtgcgagaa atgtactcca cagcttcagc
     4021 gggacgggta atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg
     4081 actttcaggc ggtaagggtt cacgattaaa aacatccaac attgcgcctt taactttgcc
     4141 gctatccagc gccgcgagca ggtcatcttc cacaacatga acaccacgcg ccaggttgag
     4201 gagatacgcg ccatccggta atttttcgag taattgttga ttaataatgc cgacggtttc
     4261 aggggtattc ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagacagttc
     4321 ttcccgtccg gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg
     4381 cagcggaaag cgccaggttt gcagactctg agcaacttta ctgcccagta cgcctgcgcc
     4441 caaaatgccg atggtaaaat cttcccgatg atattcaggc agcggttgcc aatgcgaact
     4501 attttgctgg atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc
     4561 atattcctgc atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg
     4621 gttcagcatt tcagggtgtg cctgtagctt gctcaaaata gaatcaacac cggccccgag
     4681 tgcgaacacc gctttaagat cgcgccctgc cagcatttca acaggaggat gccagactaa
     4741 agcataatca gcagagtcat tatctccgct tttccatgct ctgactcttg cctgaggaat
     4801 agctttgcgc agtgcctcaa tccaccattg ggtatcgaac gttgggtgat aaaagatgat
     4861 atccatactg actcccgaaa agcgttgtgc gaaatttatt cgcacttatc gttatgatct
     4921 acaaaggcca ccagcataac aaatccgtgg tcggtggcaa aaaaagcaga tttcgcttat
     4981 taaaaccaca cattgattga agtttgaata aacgcgcgat tttttcaaaa agtttgttga
     5041 cctcaggtca tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg
     5101 gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg
     5161 ggggttcgaa tccccccctc accgccatat ttaaagaaga gctcgtacga aagtacgggc
     5221 ttttttttcg tatattgcac acaccggggg gatgagaagc cccgaccggg gttcgacaac
     5281 tggcgacagc cagttggaca gaccgtgaac gcagtgagcg ggctgcccgc agggcgagcg
     5341 aagcgagtca atccccccct caccgccatc tttaaagaag agctcgtacg aaggtacggg
     5401 ctttttttcg tatattgcac acaccggggg gatgagaagc cccgaccggg atacttagtt
     5461 aatgcaccgg ttcactcttt gtcgccgcga ctaatgggcg aattttgtca cgagtctccc
     5521 agaccggagg aatttcctcc aatttagtga cagaagctcg tacaaaaggc acccgtgcaa
     5581 gagtcataat tgtatccaac caacggtcac gaataatgcg atccgccctt tcatgactcg
     5641 ggtcatccag ctcaataaca cagactattt tattgttctt ctttgaaacc agaacataat
     5701 ccattgccat gccattataa attgcaaaga aacgctttct ttcttcttcg ctaaactcag
     5761 gttgttctgg aaagttgatc aagcgcccca gagagacctg gcaatggata tgacaataat
     5821 gtccaaacca tttttccaga ttatcgaaat acattttttc acaacgcgtg attaaccact
     5881 cttttctttc caataaaata ccttcggcaa ccattccctg aaataactca tctcgctctt
     5941 gccaacgaga gtcacgatag gttttctgcg gtatatagct ccttgtcgac gccttagcgg
     6001 caggggaagt tgcagatgaa gggacgggtt gcgatttagc gatgattacc gatgaggcaa
     6061 cttttcggag tgtcaatttt ctggcaacag caaaaccggc gataaatgca attgtcaaac
     6121 aagttacaaa agccataaag ctcataatta attttcacct atccatgttg attatatttc
     6181 attaccaatg taactcgctg cagcgtgtta catgccaaca atgcaacgat caatatatag
     6241 ttagtatcat gttttgatta ttacaaccat ccctatttag ccattgcggc ttgccttcca
     6301 caaaaaaata acaacacaca atattagcaa agaattaatt aagattaacc ttacatttat
     6361 aatattatcc ctaaagaata aatcagagga atatattcgg tgttaaacag tcagtcaaag
     6421 tatagcccat tgttgattaa acctcataag caaaattggc accagatctc ctgtacaggt
     6481 tgaatggccg aaaaaagata ggcagatgct catttttttg gcttctggaa gaaaaactct
     6541 ccgcgctggc agtattacac tggtctgatg ctatgacgaa aaatgcctgg taagagtatc
     6601 taaaccagac tccagttcag cttatcaaca cgctcaaagt cgcccccctg aacgctaacc
     6661 tgaagtgaaa atctgttgaa ataaaacaaa gcccggttcg cccgggctct gcaccgataa
     6721 cacacttaac tgtaggcatg cagcgtacgt tggcaaagtg ccgaacgtac gcagtcctct
     6781 ttaccgaacc ggacgatccc aaccatttca tcttcttcga aacgttccag cgcgtcactt
     6841 aatccggagc acacgccgcg aggcaaatcg cattgcgtga tatcaccgtt gacgataacc
     6901 gtcacgttct ccccgaggcg ggttaaaaac attttcattt gcgcggcagt cacattctgc
     6961 gcctcgtcaa gaatgacgac tgcattttca aaggtacgtc cacgcatata ggcgaacggc
     7021 gcaatttcca ccttccctat ttccggtcgc aggcagtact gcataaagga agcccctaag
     7081 cgccggacca gcacgtcgta gaccgggcga aaatagggag caaacttttc tgcgatatct
     7141 ccaggtaaga agccaagatc ttcatcggct tgcagaactg gacgggtgac gataatcctg
     7201 tcgacatcct tatgtatcag ggcctctgcc gcttttgctg cgctgatcca ggtttttccg
     7261 cccccggctt cgcccgtggc gaatatcagc tgcttactct caatagcctt cagatagtgc
     7321 aattgcgctt catttcgcgc gaggatgggc gaagtatcgc gactgtcgcg ggccatacca
     7381 atggcttcta cgccgcccat ctgcacaagc gaggtgaccg attcttcttc acgctgctta
     7441 tggctgcgcg aatcccgtct cagcacacgt tttgcctcgc gacgagcttt gatcactgct
     7501 ttttgtcttc ccatagagag caccttgagt tgtttgtatt catcacacgc gccgttggca
     7561 gcgcgattat gcgcacgaac atcagagggt tggcttcctt gtaagccata gtttgctttt
     7621 ggataaaatg ccgaaaaacg gctacgcgca ccgtttacgg cgtcggtaac acatgaaaag
     7681 aaaggatgag gttgaaaatg caaagtgacg agatgactac cggaggagaa aactccgcga
     7741 gtggtggcgc gttgattatc taaaacatgt ccagtacagg acgttaccat ccgcgatctc
     7801 catagtgact gactatcact gccgggaact tccgctgcta cttaataagt acaacagatc
     7861 tcgcatttat tgcaacaata tatttactta tatttaacta taaaacacta tttcagtgac
     7921 attagtttct actggaaaga tgacagagtg atgacagtga tgaaaaaagc tgtgtgcttt
     7981 cagcaggatt tgcatcaggc atcagtgcac gatcgccgga tgcggcgtga acgccttatc
     8041 cagcctacca aacccgcact gattgtaggc atgataagac gcgcaagcgt cgcatcaggc
     8101 atctgcgcac aaatgcctta tccgcctaaa aacattaaac ccgcaataac gcgcttccga
     8161 gataatcgtt cgcgtccttc actcccggca gcgaaaaaaa ataaccgccg ccgataggtt
     8221 taacgtattc ctccagcgct tcgccattga gccttttttg tactgtcagg aagccttttt
     8281 ccagatcgtg ttggtagcag acaaacagca accccatatc cagttgtccg gagttggtga
     8341 cccccaacga ataactgtag ccgcgacgca gcatcaggct ggactcactc tccgccgtgc
     8401 ggggattcgc cagccggata tggctgtcca gcgcaatcac cttcccttcc gggtcgctgg
     8461 cgtaatcagg cacatcatgc tcatgctgca ttcccagcgg cgcaccggtt tgcttatcac
     8521 ggccaaaaat cgtctgctgt tctttcagcg gcgttctgtc ccaaaattcc actcgaaact
     8581 gaatcaagcg tactgcctga tagctgccac cgattgtcca cgcaggctcc tgctgatctg
     8641 ccgttaccca caccactttt tgcatcaact tatcattctg gctatcggga ttggcagtgc
     8701 cgtctttgaa acccagcaaa ttaatcggcg tctctttgcc tttactacgc gacacgtgat
     8761 cggaaataaa cccttcccgc ttccagcgca cactgagcaa atccggcgtg tgtttgatga
     8821 tatcgcgcag cgcatggata accgtgtcct gggtgttggc gcaaatctgt agcaacacat
     8881 caccatgaca taacgccgca tccagcgagt cgttggggaa acgcgtcatc ttctgcagct
     8941 tttttggcat ctgtggcgca aggccaaagc gctcatcaaa caatgagtga cccaccgata
     9001 acgtgatggt gagattatcg ggcgcaatgt agccgccaag aatgccggaa tcgagtggtg
     9061 gcaggcgcgg atttggcgtt tctggtgctg ctccgccctg agtcagaaaa gcaaaacgct
     9121 gagtcaacaa gcgaaacaac cgctcaagat cggctttatc gctggcaagc acatcaaacg
     9181 ccaccagcat cattgcggcc tgttgtggcg tcaggatccc tgcctgatgc tcaccataaa
     9241 acggctgttt ctcattgcgt gcatccggtg aaagcgtgcc cggtgcactt tgcgtttttt
     9301 gtgcatgagc gaccggacaa cttcccgcca gcgccagcgc accaatccct ttcagtaaac
     9361 gtcggcgtga cggttcattc acgccgtttt catctttata ctgcataacg cttaatccag
     9421 tcccagcaca ccgcgaagtt gcgccagatc ttccgccagc gcagtaatcg gtcctttcag
     9481 tgcattccgg tcggcatcgg tcaatttgtc gtaggtttca aaaccgtctt tagtacggta
     9541 tttcgccaga atggtatcga cctttttaaa gttggcatcg acttttgcca gcagttccgg
     9601 gttggctttt tgcagttgtg gacgcagcaa atcgacaatt ttctgcgagc cttcaacgtt
     9661 agcctggaaa tcccacagat cggtgtggct gtagcgatct tcttcaccgc taattttgct
     9721 ggctgccact tcctcaatca gtccggctgc gccgccgacc acttttgaag gtgggaaagc
     9781 cagttcactg atgcgttttt gcaaatcgac cacatctgta taaagctgct cagcgtactg
     9841 atccatccct ttggtggtgt tgtcgccaaa caatgctttt tccagacggt ggaaaccagt
     9901 gaattttggg tcggcggctt tttgctcgta atcatcttca cgggcgtcaa tgctgccatc
     9961 cagatcggag aacagttcag caatcggttc aatgcgctca tagtgctggc gcgtcggcgc
    10021 atacagtgct ttcgcttttt cgatatcgcc tgctttaatc gcgtcggtaa aggctttggt
    10081 gtcggtcacc agctgcgtgg tttccgccat gacatacgct ttatatgcag taattgcacc
    10141 accaagactt aacagcgcat cactttgcgc cgcatccgcc gttgcctcac ctttgacgat
    10201 caacttccct ttcgggttag tcagcagacc gcaggtcata tcgtattcgc caggctgtaa
    10261 attcgccgtc attttctggc taaaaccagg ggcgatattt tcccgctctt ccaccaccat
    10321 cacgcctttg aggatctccc actccagcgc cttctggcta tggttctgaa taatgaactg
    10381 tgttttcccg gcgttaaccg taatggtcat cggttcgcac tgcttatccg tcacggtcac
    10441 tttgacctgc ggcacatcag cggcgttagc cataaaagca gaagaaaaca gcgcagccac
    10501 gctcaactgc aatgcgttac ggcggaagtt aatggtcatg acgagactat ccctttaaag
    10561 tatgttgtaa ctaagcaaga gtttgcgtcg tatttgttac gcggagcgag acgctgtcgc
    10621 ccctgcgcgt ggtggcagag caaatgccac cagcgccggg atgagataaa taaaccagac
    10681 ggcgacttcg ctgacgctcg gtgcttcctg atagccaaaa atcccttcca tcagcgtgcc
    10741 aaacagcgag tgagttgaga gcactgcgct catatcgaag gcgatttcct gaaagtggtt
    10801 ccacaatccg gcttcatgaa atgcgcgaat ggcaccagca gccagccctg cggcgacgaa
    10861 gagaataaac aggctggtcc atttaaaaaa tgcaccaaga ttgaggcgaa taccgcccca
    10921 gtagagcagg aagcctagca ccacggcagt agcaagaccg agcattgcac ccagcggcgg
    10981 ccagatcccg acatcttgtt gaaatgccgc cagcaggaaa aagaccgact ccagcccttc
    11041 ccttgcaacg gcaaaaaaga ccatcatcac cagcgcccag ccatgatgat ttccacgctg
    11101 caatgcgcta tcgactgcct gctccagttg tactttgacg ttgcgcgaca ctttgcgcat
    11161 ccagaaaacc atccaggtaa ggatcaccac ggcgatcacc gccacgatac cttcaaacag
    11221 ttcctgttct ttttgcggaa attcgccggt ggtttcgtta atgaagatac ccaagcccag
    11281 gcacaacgca gcggcaagca acacgccaat ccacatcacg ccaatccatc ggcctcgctg
    11341 ggtacgctta agatagctgg caatcaaact gacaatcagc gcggcttcaa gtccttcgcg
    11401 caacataatg agaaacggaa caaacatgcc acccaccctt aaacgttttt ctgagtcaat
    11461 ccatgcaaag aaaagtaaat cgcagtgata gcgattatca ttacggcaaa gaaaaataca
    11521 agcggaatgt ttataaaatg atgttggaat gtaaacaaag agaggtaata ccaggggata
    11581 taaacgatta ttccaggtac aaagatggga ttgtttacaa cgagccatgt gttcttgcca
    11641 gatgccgcgt aaacgcctta tccagccgac aaaactgtcc agactcaatg catcacagct
    11701 tccatgtaaa actgataagc gggtgtatca agcaatttcg tccatccgcg attatcaaat
    11761 ctaacttttt gcccggaaca taataacagc gcgggtgttg gtggcgtcaa aatcgctgaa
    11821 acgttccccg aaggccgggg cagcccacat ggatgtgggc tgagggcgcg tcttacagga
    11881 cgttacctcg cgcccgaccc ggtagccgaa gggga
//
LOCUS       DAJTYZ010000117        11912 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000117 DAJTYZ010000000
VERSION     DAJTYZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.117"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..179
                     /gene="bdm"
                     /locus_tag="OO596_003173"
     CDS             <1..179
                     /gene="bdm"
                     /locus_tag="OO596_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR6718961.1"
                     /translation="PALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILSDIYQ
                     QTINYVVSGQHPTL"
     gene            281..418
                     /gene="sra"
                     /locus_tag="OO596_003174"
     CDS             281..418
                     /gene="sra"
                     /locus_tag="OO596_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR6718962.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            575..2272
                     /gene="maeA"
                     /locus_tag="OO596_003175"
     CDS             575..2272
                     /gene="maeA"
                     /locus_tag="OO596_003175"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6718963.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2406..3408
                     /gene="adhP"
                     /locus_tag="OO596_003176"
                     /pseudo
     CDS             2406..3408
                     /gene="adhP"
                     /locus_tag="OO596_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3554..3838
                     /locus_tag="OO596_003177"
     CDS             3554..3838
                     /locus_tag="OO596_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6718964.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4066..4719)
                     /gene="fdnI"
                     /locus_tag="OO596_003178"
     CDS             complement(4066..4719)
                     /gene="fdnI"
                     /locus_tag="OO596_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6718965.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4712..5596)
                     /gene="fdnH"
                     /locus_tag="OO596_003179"
     CDS             complement(4712..5596)
                     /gene="fdnH"
                     /locus_tag="OO596_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6718966.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5609..8656)
                     /gene="fdnG"
                     /locus_tag="OO596_003180"
     CDS             complement(5609..8656)
                     /gene="fdnG"
                     /locus_tag="OO596_003180"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8069..8071),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6718967.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8888..9769
                     /gene="yddG"
                     /locus_tag="OO596_003181"
     CDS             8888..9769
                     /gene="yddG"
                     /locus_tag="OO596_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6718968.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10028..11128
                     /gene="ompC"
                     /locus_tag="OO596_003182"
     CDS             10028..11128
                     /gene="ompC"
                     /locus_tag="OO596_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6718969.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            11292..11774
                     /locus_tag="OO596_003183"
                     /pseudo
     CDS             11292..11774
                     /locus_tag="OO596_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3022 a         2995 c         2897 g         2998 t
ORIGIN      
        1 aacccgctct ggttaatgcc attgagcaag gccttcgggc agagcaaggt gttgtcactg
       61 aagatgacat tctcatggag ctgaccaaat gggtggaagc ttctgataac gacatcctca
      121 gtgatatcta ccagcaaacg atcaattatg tggtcagtgg ccagcaccct acgctttaag
      181 gtgctatgct tgatcggcaa cctaatttag gggtttagca cgtgtttctt cgctacggcg
      241 atgttgtcct taaaactagc aacaggattg aggagttaaa atgaaatcga accgtcaggc
      301 acgtcatatt cttggactgg accataaaat ttctaaccag cgcaaaatag ttaccgaagg
      361 tgacaaatcc agcgtggtaa ataacccaac cggcagaaaa cgccccgctg aaaagtaatt
      421 cataaccatc agtcctcaat gacgattaaa caccattgcc tgcgcaatgg tgtttttgtt
      481 tttatctgct ttatacttgg ggccgacgcc ctggcggtaa agcaaagacg ataaaagcgt
      541 gccagggatg gatattcaaa aaagagtgag tgacatggaa ccaaaaacaa aaaaacagcg
      601 ttcgctttat atcccttacg ctggccctgt actgctggaa tttccgttgt tgaataaagg
      661 cagtgccttc agcatggaag aacgccgtaa cttcaacctg ctggggttac tgccggaagt
      721 ggtcgaaacc atcgaagaac aagcggaacg agcatggatc cagtatcagg gattcaaaac
      781 cgaaatcgac aaacacatct acctgcgtaa catccaggac accaacgaaa ccctcttcta
      841 ccgtctggta aacaatcatc ttgatgagat gatgcctgtt atttataccc caaccgtcgg
      901 cgcagcctgt gagcgttttt ctgagatcta ccgccgttca cgcggcgtgt ttatctctta
      961 ccagaaccgc cacaatatgg acgatattct gcaaaacgta ccgaaccata atatcaaagt
     1021 gattgtggtg actgacggtg aacgtattct ggggcttggt gaccagggca tcggcgggat
     1081 gggcattccg atcggtaaac tgtcgctcta taccgcctgt ggcggcatca gcccggcgta
     1141 tacccttccg gtggtgctgg atgtcggaac gaacaaccaa caactgctta acgatccgct
     1201 gtatatgggc tggcgtaatc cgcgtatcac tgacgacgaa tactatgaat tcgttgatga
     1261 atttatccag gctgtgaaac aacgctggcc agacgtgctg ttgcagtttg aagactttgc
     1321 tcaaaaaaat gcgatgccgt tacttaaccg ctatcgcaat gaaatttgtt cttttaacga
     1381 tgacattcag ggcactgcgg cggtaacagt cggcacactg atcgcagcaa gccgagcggc
     1441 aggtggtcag ttaagcgaga aaaaaatcgt cttccttggc gcaggttcag cgggatgcgg
     1501 cattgccgaa atgatcatcg cccagaccca gcgcgaagga ttaagcgagg aagcggcgcg
     1561 gcagaaagtc tttatggtcg atcgctttgg cctgctgacg gacaagatgc cgaacctgct
     1621 gcctttccag accaaactgg tgcagaaacg cgaaaacctc agtgactggg ataccgacag
     1681 cgatgtgctg tcactgctgg atgtggtgcg caatgtaaaa ccagatattc tgattggcgt
     1741 ctcaggacag accgggctgt ttacggaaga gatcatccgt gagatgcata aacactgtcc
     1801 gcgtccgatc gtgatgccgc tgtctaaccc gacgtcacgc gtggaagcca caccgcagga
     1861 cattatcgcc tggaccgaag gtaacgcgct ggtcgccacg ggcagcccgt ttaatccagt
     1921 ggtatggaaa gataaaatct accctatcgc ccagtgtaac aacgccttta ttttcccggg
     1981 catcggcctg ggggttattg cttccggcgc gtcacgtatc accgatgaga tgctgatgtc
     2041 ggcaagtgaa acgcttgcac agtattcgcc gctggtgctg aacggcgaag gtctggtact
     2101 accggaacta aaagatattc agaaagtctc ccgtgcaatt gcgtttgcgg ttggcaaaat
     2161 ggcgcagcag caaggcgtgg cggtgaaaac ctctgccgaa gctttgcaac aagccattga
     2221 cgataatttc tggcaagccg aataccgcga ctaccgccgt acctccatct aagcctgcgc
     2281 ccggtagtga aggctaccgg gcaattttcc tctccctttt tcagatctca tccatactgg
     2341 gtagtggcga ataaatctca tttgcctcac ctgctatgca gaacatcatc cgaaaaggag
     2401 gaactatgaa ggctgcagtt gttacgaagg atcatcatgt tgacgttacg gataaaacac
     2461 tgcgctcact gaaacatggc gaagccctgc tgaaaatgga gtgttgtggt gtatgtcata
     2521 ccgatcttca tgttaagaat ggcgattttg gtgacaaaac cgacgtaatt ctgggccatg
     2581 aaggcatcgg tgtggtggca gaagtgggtc caggtgtcac ctcattaaaa ccaggcgatc
     2641 gtgccagcgt ggcgtggttc tacgaaggat gcggtcattg cgaatactgt aacagtggta
     2701 acgaaacgct ctgccgttca gttaaaaatg ccggatacag cgttgatggc ggaagagtgc
     2761 atcgtggtcg ccgattacgc ggtaaaagtg ccagatggtc tggactcggc ggcggccagc
     2821 agcattacct gtgcgggggt caccacctac aaagccgtta agctgtcaaa aattcgtccc
     2881 gggcagtgga ttgctatcta cggtcttggc ggtctgggta acctcgccct gcaatacgca
     2941 aagaatgtct ttaacgccaa agtgatcgcc attgatgtca atgatgagca gttaaaactg
     3001 gcaaccgaaa tgggtgcaga tttagcgatt aactcacgca ccgaagacgc cgccaaaatt
     3061 gtgcaggaga aaaccggtgg cgctcactct gcggtggtaa cagcagtagc taaagctgcg
     3121 tttaactcgg cagttgatgc tgtccgtgca ggcggtcgtg ttgtggctgt cggtctgccg
     3181 ccggagtcta tgagcctgga tatcccacgt cttgtgctgg atggcattga agtggtcggt
     3241 tcgctggtcg gcacgcgcca ggatctaacc gaagccttcc agtttgccgc cgaaggcaaa
     3301 gtggtgccga aagtcgccct gcgtccgtta gcggacatca acaccatctt taccgagatg
     3361 gaagaaggca aaatccgtgg ccgtatggtg attgatttcc gtcactaaga ggtctttgct
     3421 gcgactccca tgttcgggtc gcagcatcgc acactctcca acatgaaatg gctaaatgga
     3481 ttattgttgc gtaggaagga acaataatga tagtgttccc agagaaggaa cgctactgag
     3541 agtaaagaca ctaatgaaaa tggccaatca tccccgcccg ggggacatta ttcaggaatc
     3601 actggacgaa cttaatgtca gcctgcgcga gtttgccaga gcaatggaaa ttgcgccctc
     3661 aacggcaagc cgattgctga ccggaaaagc agctttgacg ccagaaatgg caattaaact
     3721 ttccgtggtg atcggcagtt cgccgcaaat gtggctgaat ctgcaaaacg cctggagtct
     3781 ggcagaggca gaaaaaacgg tggatgtgtc gcgattacgc cgtctggtta cgcaataaaa
     3841 aatggcacct tataaagcgc catttgaaac tgatgacaaa cgcaacattg cctgatgcgc
     3901 tacgcttatc aggcctacac gttcacttca atctattgta tttgaagggt tttcatgccg
     3961 gataaggcat tcacgacgca tccgggatga acaaagccca cgttgtctac aatatgaaaa
     4021 tggcgccttt tacagcgcca ttttcagctt aaagttcaaa agagattata tcccttcttc
     4081 actctctttt ttcgcttctg ccttctcgat ttcacgatac cagcgcggat ggtgtttctt
     4141 cgcccagcga cggctcacct tcccttcgat catcccttta atcgaccctt tcacccagaa
     4201 tgccatatac atatggatca ggatggcgtg gatcaggatg atacccgcag ccgcgtggat
     4261 cagcaggctg tagcgaacaa cctgcatcgg gaagtactgc gcaaagtacg gacgccagat
     4321 aatcaccccg gtcaccagca gcacgaaaat catgctcatg atcgaccaga acatcatctt
     4381 ttgcccggcg ttgtacttac caacatccgc cactttgtgc tcattgcctt ttaatacttc
     4441 gacaatgttc aacagccacg gaatatcttt cttatccggg atgttgtgat gcacaaaacg
     4501 cacaaacata aacatcagtg cgacgaaaat cgcaatgccg aagaacgggt gcaaaatgcg
     4561 tcccatctgc ggcgtaccga aggtttgcgt cagccattgc agcgtcggga agaagaacga
     4621 aatcccggac agcgccacca ggaagaagca aatcaccacg gtccagtgac aggcgcgatc
     4681 aataaatttg gtgcgcacaa tcattttcga cttactcatg atgatcctcc tcgtcatcgt
     4741 ccacttcctt attcgggcca ataccgatgt agtggaaaat caacccggca aaagtggcaa
     4801 taaagccagc cgctgccagc ggtttcaacg cgcctttcca caggcttacc gaggtgtcga
     4861 tcttcggatc tttcggcaga ccgtaataca gctccggctg atcggcgtga tgcagcacgt
     4921 acataacgtg cgtaccaccg acccctttcg ggttgtagac gccagcatgt tcgtaaccac
     4981 gcgctttcag tttcgccacg cgctcttccg ccagctccag catctccttc ttggtgccga
     5041 agtggatagc cccggtcgga caggttttca cacaagccgg ttcctggccg acgctgacgc
     5101 gatcgacgca gagcgtgcat ttatataccc ggttatcctc tttgttgagg cgcggaatat
     5161 taaacggaca cccggcaatg cagtaaccac agccgatgca gttttccgac tggaaatcga
     5221 caatcccgtt agcgtactga atgattgcac cagcagacgg gcacgccttc aggcagccgg
     5281 gatcttcaca gtgcatacag ccgtctttac ggatcagcca ctccagcttg ccgttctgtt
     5341 cggtttcgct aaagcgcatc accgtccagg acttggcgct cagatcggcg gggttatcgt
     5401 aaaccccgac gcagtgcccc acttcatcac ggatgtcgtt ccactccgaa cacgccacct
     5461 gacaggcttt acagccgata caggtggaaa cgtcgataag ttttgcgact tctgctttgt
     5521 aatcacgcac ctgagaaggc ggcgtgatgg agttagttgc ggaccttttg ataatatcct
     5581 gcgtttccat agccatctgt tcgccccctt acgccttctc gatgttgact aagaacgctt
     5641 tatattccgg cgtttgcgag tttgcatcac cgacattcgg cgtcagagtg ttagcgatat
     5701 aacctttacg cgcgacaccc tcaaagcccc agtggattgg aatacccacc gtttcaacct
     5761 gctgaccgtt tacattcagc ggtttcagac ggcgcgttac caccgccacc gcgcggataa
     5821 agccacgctt gcttgagaca gtgacacgat cgccattatt aatgcctttc gccgccgcca
     5881 gcgtttcgct gatttccaca aactgttccg gctgagcaat tgcgttgagc aatgcgtgct
     5941 tggtccaggt gtggaagtgc tcggtcagac gataggtcgt acccacatac gggaactgct
     6001 cttttttacc catccgcagc gcgtcttgtt catacagacg aacaaccggg ttagacacca
     6061 cgttcggatg cagcgggtta gtgcccagcg gcgtttcaat cggctcgtag tgttccggga
     6121 acggaccttc cgccattttg ttgatggcaa acaggcgtcc catcccttcc ggctgcatga
     6181 taaacggccc ggttggcgta cccggtgcgg cattgccgaa gtccggaata tcgttacccg
     6241 tccacttgct gccgttccac tggatcagca tccgtttcgg atcccacggt ttaccattga
     6301 tatccgccga agcacggttg tagagcacgc gacggttgag cggccacgcc caggcccatc
     6361 ccagcgtatt ccccagaccg gatgggtcgg agttatcgcg gttagccatc tggttgccct
     6421 gctctgtcca gctaccggtg tagatccagc aagaagatgc ggttgtaccg tcatcacgca
     6481 gatgcgcaaa gctactcagc aactgacctt tcttcgcaat cagcacgcca ttagcgtcat
     6541 agagatcttc cagcgcatag ccgttattct ctttcgccac ttcgtcagat tgcggttcgt
     6601 gcggctgctt gtagttccag ctcatcttca tcagcggttc tacgccttta ccaccttcgg
     6661 cctggtacag ctcgcgcaga tgatggtaga tacccgccag aatttcgccg tcgttacgcg
     6721 cttcgcccgg cgcgtcctga cctttccagt gccactgcaa ccagcgaccg gagttagcga
     6781 tagaaccatc ttcttcggca aagcaggtcg aaggcagacg gaatacttca gtctgaatag
     6841 acgccggatc gacatcgttc gactcgccgt ggttctgcca gaaggtagag gtttcagtca
     6901 ccagcggatc gataaccacc atgtacttca gcttgctcag gcagctcacc actttgtttt
     6961 tgtccgggaa ggacgcaacc gggttaaagc cctggcagaa gtaaccggtg actttgcctt
     7021 cgtccatcat gttgaaatac ttgatgacgt cgtaggtctg gtcccacttc ggcagccagt
     7081 catagcccca gttgttctct ttctgcgcgg catcgccata gaaagatttc atcaggctaa
     7141 cgaagaactt cggatagttg ctccagtagt tcacctgatc agccagcgtc gctttcggcg
     7201 tgttcgcttc cagatacgac tgcaaatcaa cctgtttttc tgacggaagc gtcagataac
     7261 ctggcaggct ggtagagagc agacctaagt cagtcagccc ctgaatgttg gagtgaccac
     7321 gcaatgcgtt cacgccgcca ccggccatac ccatgttacc gagcagcaac tggatcatcg
     7381 ccatagtacg gatgttctgc gcacccacgg tgtgctgcgt ccagcccagc gcgtacagga
     7441 aggtggttgt gcgatccggt gcgctggtgg aggccagcac ttcacacact ttcaggaagt
     7501 cggcttttgg cgtaccgcag atgttttcaa cgacgtccgg cgtgtagcgg gaaacgtgcg
     7561 ctttcagcag gttccacaca cagcgcggat gagtcagtgt ttcatcgcgt tttgcatagc
     7621 cgttttcatc gaactgatag ttccaggacg acttatcata ctggcgtttt tcagcgtcgt
     7681 agccgctgaa caggccgtct tcgaaagcaa aatcatcacg caccagcagg ctggcgttgg
     7741 tgtaatgttt aacgtattcg gcgttgattt tgttgttttc gatcaggtag cgcaaaacgc
     7801 cagacaggaa cgtaatgtcc gtaccggaac gaataggtgc gtaaatatcc gccacagaag
     7861 cggtacgcgt aaaacgagga tcgacaacga tcaaggttgc atcgttgttg tttttcgctt
     7921 ccatcgccca gcggaaaccg acgggatgcg cttcagcagc gttaccgccc attaccatta
     7981 cgacgttagc gtttttgata tccacccagt ggttggtcat cgcaccgcga ccaaatgttg
     8041 gagcaagact tgctaccgtt ggtccgtgtc agacgcgcgc ctggttgtct accgccagca
     8101 tcccgaggga gcgggcaaat ttctgggtca gcatcccggt ttcgttgctg gcaccggagg
     8161 cacacagcat accggtagaa agccaacggt ttaccgttac gccctgctcg ttcttttcaa
     8221 taaagttagc gtcacggtca gctttcatca gcttcgcaat acgggagaat gcttcttccc
     8281 agctaatgcg ctgccatttg tcagaacctg gcgcacgata ttccgggtag cgcagacggt
     8341 tttcgctgtt gacgtaatcc agcaaaccgg ccccttttgg gcacagcgca ccacggctta
     8401 ccggatgatc cgggtcacct tcaatgtgat aaatcgcttc tctggcgttt ttcgcgccat
     8461 cacccaggct atacatcaat agcccgcaac ctacggaaca gtatgtgcag gtgttacgga
     8521 tctctttagc gcgtaataat ttgtagtttc gcgcctgagc cagtgcttgc ttcggggcaa
     8581 agcccaatgc cgctaccgtt gttccagcca taccgcccgc gcagatttta aaaaattgtc
     8641 tgcgactgac gtccattgct ttcctctttt ttcaggggta ttactgcgcg aggaaactaa
     8701 cagtaaaatt ctgaatcttt ttgcgcgaaa tcaaaaagaa cacgtgtatg ccacaaaata
     8761 atccgcctgc gcaaattaac ctacctcaat agcggtagaa aaacgcacca ctgcctgaca
     8821 ggccagttaa aaaaatgcta taaaattcag cttaattttt aacggcaaga gagacaaaac
     8881 agcgagcatg acacgacaaa aagcaacgct catagggcta atagcgatcg tcctgtggag
     8941 cacgatggta ggattgattc gcggtgtcag cgaggggctc ggtccggtcg gcggcgcagc
     9001 tgctatctat tcattaagcg ggctgctgtt aatcttcacg gttggatttc cgcgtattcg
     9061 gcagattccg aaaggctatt tactcgccgg gagtctgtta ttcgtcagct atgaaatctg
     9121 tctggcgctt tccttagggt atgcggcgac ccgtcatcag gcgattgaag tgggtatggt
     9181 gaactatctg tggcccagcc tgacaattct ctttgccatt ctgtttaatg gtcagaaaac
     9241 caactggctg attgtaccag gattattatt agccctggtc ggcgtctgct gggtgttagg
     9301 cggtgagaat gggttacatt atgatgaaat catcaataat atcaccacca gcccattgag
     9361 ttatttcctg gcgtttattg gtgcgtttat ctgggcagcc tattgcacag taacgaataa
     9421 atacgcacgc ggatttaacg gaattaccgt ttttgtcctg ctaacgggag catgtctgtg
     9481 gatttactat tttctcacgc cacaaccaga aatggtattt agcacgcccg tcatgattaa
     9541 actcatctct gcggcattta ccttaggatt tgcttatgct gcatggaatg tcggtatatt
     9601 gcatggcaat gtcaccataa tggcggtagg ctcgtatttt acgcctgtac tttcctcagc
     9661 gcttgcagcc gtgctgctca gcgccccgct gtcgttctcg ttctggcaag gcgcgctaat
     9721 ggtctgcggc ggttccctgc tctgctggct ggcgacacgt cgtggttaaa ataattgccg
     9781 ggtctacggc ccggcagtta atcagataaa ttccgacaat attaaaaaat aaatttcaaa
     9841 taccagtaat tcaccttata tctcatttcg acttcgatca catatcgata aataatatta
     9901 tttcatctat ttttctcatt gtttttaatc accattctat tttaaaaaca gtatgtcatt
     9961 tataaatgac gccgtttaaa attcgtttag aaaatacgtt taacgataat taaaaggatt
    10021 ctgttaaatg aaattaaaaa tagttgcggt ggttgtaact ggtttgttag ctgcgaacgt
    10081 agcacacgct gccgaagtct ataacaagga tggtaataaa ctcgaccttt atggcaaggt
    10141 taccgctcta cgttatttta ctgatgataa gcgtgacgat ggtgataaaa cttatgcccg
    10201 tctcggcttt aaaggagaaa cgcaaatcaa tgatcaaatg attggttttg gtcactggga
    10261 atatgatttt aaaggctata acgatgaagc caacggctcg cgcggcaaca agacccgtct
    10321 ggcctatgct ggtttaaaaa ttagtgaatt tggctctctg gactatggtc gtaactacgg
    10381 tgtcggctac gacattggtt catggaccga tatgttgcca gaatttggtg gcgatacctg
    10441 gagtcagaaa gatgtcttca tgacataccg taccaccggt ctagcaacct atcgcaacta
    10501 cgatttcttt ggcttaattg aagggctgaa ctttgccgcg caatatcaag gcaaaaatga
    10561 acgtactgac aacagtcatc tttatggtgc tgactacacg cgtgccaacg gtgacggttt
    10621 cggtatctcc tcaacttatg tttatgatgg ctttggtatc ggtgcggtat ataccaaatc
    10681 cgatcggaca aatgcgcagg aaagagccgc tgctaatcct ctcaatgcct ccggtaagaa
    10741 tgcagaacta tgggccacag gtataaagta tgatgccaac aacatctact ttgcagctaa
    10801 ttacgctgaa acattaaaca tgaccaccta tggggatggt tatatctcta acaaagcaca
    10861 aagttttgaa gtggtggcgc aatatcaatt cgacttcggc ttgcgcccat cactcgctta
    10921 cctgaaatcg aaaggcagag atctgggccg ctacggcgac caggacatga ttgagtatat
    10981 cgacgttggt gcgacgtatt tcttcaacaa aaatatgtcg acctatgttg attataaaat
    11041 caacctgatt gatgaaagcg actttacccg tgccgtagat attcggaccg ataacatcgt
    11101 cgctacgggc attacctatc agttctaacg tcctctcctg accagccctt ctctgggctg
    11161 gtttttgatt taagtgctgc tgtatgactc cttaagtaaa aatgagcaat ttttgatatt
    11221 tctggtcatt cacctcaatt aatattattc catatcctcc ggggacatct tactataata
    11281 tttattgatg agtgattatg accaatatta atacagcttg tgtaaaaaat aatgccagtt
    11341 atcaacttaa taacgcatta cctaacaaag aaaccatctc cagcaatttt tgtgaacgac
    11401 tagaacaatg gggtaataaa tcggttaata atggtgaaga aagagcaatt gccgtggagc
    11461 gaattaaaga agcttacaat tcgaacatgg catctcttga tttgtcatat cttgatttga
    11521 gtgaattacc gcctatcccc tcgacagtta atacgttaaa tctggaaaac aattgtctaa
    11581 cttgtcttga cttagctgac aatgccagtc tcgttaatat caacctcagc gttaataaaa
    11641 ttaacacgat aacctttcca aaagaatcaa aactggaaaa catttatatt gatcataaca
    11701 atctggaaag tttagattta aaaaatcagc attcattggt taatctggaa gcgcaaaata
    11761 acaaactgac aaaaattaat atttctgata gttataaact gaaatttctt aatcttgatt
    11821 ataacaagct agcatcgctg gatgtctccc ggcaagaatc tctgattgag ttaagtgccc
    11881 atcacaatat gatcactgac cttatattac ac
//
LOCUS       DAJTYZ010000118        11867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000118 DAJTYZ010000000
VERSION     DAJTYZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11867
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.118"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1750
                     /locus_tag="OO596_003184"
     CDS             <1..1750
                     /locus_tag="OO596_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCR6718970.1"
                     /translation="INVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITP
                     KKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFH
                     AYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSA
                     DIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNIS
                     KDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHE
                     QIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYERDD
                     LSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFN
                     DFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKG
                     CSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINK
                     GNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRS
                     ALLLGKTGEPPNYLYL"
     gene            2086..3063
                     /gene="csiD"
                     /locus_tag="OO596_003185"
     CDS             2086..3063
                     /gene="csiD"
                     /locus_tag="OO596_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6718971.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3083..4351
                     /gene="lhgO"
                     /locus_tag="OO596_003186"
     CDS             3083..4351
                     /gene="lhgO"
                     /locus_tag="OO596_003186"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6718972.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4374..5822
                     /gene="gabD"
                     /locus_tag="OO596_003187"
     CDS             4374..5822
                     /gene="gabD"
                     /locus_tag="OO596_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6718973.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5836..7116
                     /gene="gabT"
                     /locus_tag="OO596_003188"
     CDS             5836..7116
                     /gene="gabT"
                     /locus_tag="OO596_003188"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR6718974.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7427..8743
                     /locus_tag="OO596_003189"
     CDS             7427..8743
                     /locus_tag="OO596_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6718975.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSA
                     LYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKF
                     LIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVV
                     MLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            8764..9426
                     /gene="csiR"
                     /locus_tag="OO596_003190"
     CDS             8764..9426
                     /gene="csiR"
                     /locus_tag="OO596_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6718976.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9427..9876)
                     /gene="kbp"
                     /locus_tag="OO596_003191"
     CDS             complement(9427..9876)
                     /gene="kbp"
                     /locus_tag="OO596_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6718977.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9960..10118)
                     /locus_tag="OO596_003192"
     CDS             complement(9960..10118)
                     /locus_tag="OO596_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6718978.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10301..10600
                     /locus_tag="OO596_003193"
     CDS             10301..10600
                     /locus_tag="OO596_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6718979.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10610..11134
                     /gene="ygaP"
                     /locus_tag="OO596_003194"
     CDS             10610..11134
                     /gene="ygaP"
                     /locus_tag="OO596_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6718980.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11181..11585)
                     /gene="stpA"
                     /locus_tag="OO596_003195"
     CDS             complement(11181..11585)
                     /gene="stpA"
                     /locus_tag="OO596_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6718981.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2960 a         2928 c         3061 g         2918 t
ORIGIN      
        1 tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa tcaatccaga
       61 acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat cacaatgtga
      121 aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg ccacctacca
      181 gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata tacaaatccc
      241 tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa agggttactg
      301 gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg gagatttgca
      361 ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca ttcctgatta
      421 tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt atccctctgc
      481 tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct attggcaagg
      541 tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac aaatccatcc
      601 agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca tctctaagga
      661 tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc ctaaaacaac
      721 acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa tgcgaaaata
      781 tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac aaatagaaag
      841 atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta acttatttaa
      901 cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa cttgtcagtt
      961 ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg ccattgattt
     1021 ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc taacccttgc
     1081 atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat tgattaacat
     1141 taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta accatcaaca
     1201 agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac ctttagttta
     1261 ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg ggtccacgcg
     1321 tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc tctacaatga
     1381 attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg gtgtatttgc
     1441 aactaaaaac gttctagcct ttagtcgtgg ggattctgtg aacattaatc actctcctca
     1501 tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct ggcctaacaa
     1561 attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta ttattaataa
     1621 tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa ctctaaatat
     1681 caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc cgaactatct
     1741 ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat atgtttagtt
     1801 tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag ataatctgtt
     1861 ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt ctcactttgc
     1921 cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata agaaaacaat
     1981 atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg gctacgaaat
     2041 gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa tgcactgacc
     2101 gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac tctcatcccg
     2161 tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac caaacagttt
     2221 ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt tctgcgtttt
     2281 cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt gctgttgaag
     2341 accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat cgatgatgtg
     2401 gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat tggtcgctca
     2461 aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa tgtcgataac
     2521 tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga cggcacgtat
     2581 gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa catgcagggt
     2641 ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta tttccgccat
     2701 ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt cagcaaagat
     2761 gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg ctatatcgac
     2821 cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct ttccgatgcc
     2881 attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt cctgttgata
     2941 aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga tctgcgccgt
     3001 gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca gacgcatcag
     3061 taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg gcatcatcgg
     3121 catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg cgttgctgga
     3181 aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga tccatgccgg
     3241 ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga accgcgccac
     3301 caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga tgctggtcgc
     3361 cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag cggcgaacgg
     3421 tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga atatcaccgg
     3481 gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag tcacagcggc
     3541 aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg aagtcagcgc
     3601 cctcagtaag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg aatatgaagc
     3661 atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa tgctcggcct
     3721 cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg cgccggagca
     3781 taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc catttttggg
     3841 cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg cggtgctggc
     3901 tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc tggagatttt
     3961 gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac tgggcgagat
     4021 gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt gtccccggct
     4081 ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag tatcgccgga
     4141 cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc acacctgcaa
     4201 tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg tcagcaaggt
     4261 acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg cacgtagtgt
     4321 ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta acaatgaaac
     4381 ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg ctggacgcca
     4441 acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg ggtagcgtgc
     4501 cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc gccctgcccg
     4561 cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg ttcaatttga
     4621 tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc aaaccactgg
     4681 ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt gccgaagaag
     4741 gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc ctgattgtta
     4801 tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg gcggcgatga
     4861 ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg aagcccgcca
     4921 gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg ggcattccgg
     4981 ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa ctgaccagca
     5041 acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc cagttaatgg
     5101 aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac gcgccgttta
     5161 tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc tcgaaattcc
     5221 gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac ggcgtgtatg
     5281 accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc gacgggctgg
     5341 ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa gtggaagagc
     5401 atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa gcggatgaac
     5461 gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac gccaaagtgt
     5521 cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat gaagccgatg
     5581 tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac gcccgtgatt
     5641 taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc atcaataccg
     5701 gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt ctgggtcgtg
     5761 aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc atcggtcttt
     5821 aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag tcaggcgatt
     5881 ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa ctgccgggtg
     5941 tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt gctcaatacc
     6001 gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa actgtcgcac
     6061 acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat tatgaatcag
     6121 aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc cgaagcggtg
     6181 gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat cgcttttagc
     6241 ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt gaatccgtac
     6301 tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc ttgcccgctg
     6361 cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa aaatgatgcc
     6421 gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg cggtttctac
     6481 gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca cgggatcatg
     6541 ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt tgcgatggag
     6601 cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg cggtttcccg
     6661 ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg cggtctgggc
     6721 ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt gaaggtgttc
     6781 gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa agacggattg
     6841 ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg agcgatgatt
     6901 gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact caccgccgag
     6961 atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc gtattacaac
     7021 gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca gggtctggag
     7081 atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt aagagttaac
     7141 cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc gaacgacaaa
     7201 gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag ggtgaggggt
     7261 aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag aaaaccctcc
     7321 cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc ggcgcgggaa
     7381 atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg ggcaatcatc
     7441 gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt tgtctattgc
     7501 cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg aagcgggccc
     7561 ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta tgcggatgtt
     7621 ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg ccgataaagc
     7681 cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt gggtactggt
     7741 tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc caggcattcc
     7801 catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt tattaagcgt
     7861 taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg ctatcctggc
     7921 ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg aagtgagcgg
     7981 gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg cggtattaag
     8041 cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca ttgccgccgc
     8101 ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta tctggcgtat
     8161 ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt ggaatatgcc
     8221 ggggctgaaa gccgttggat cttatcgctc ggccctgtat accgcctcaa ggatgctcta
     8281 ctccttaagc cgtcgcggtg atgctcccgc ggtaatgggt aaaatcaacc gcagtaaaac
     8341 cccgtacgtg gcggtgttac tctccaccgg cgcggcattc ttaacggtgg tggtgaacta
     8401 ttacgcgcct gcgaaggtat ttaaatttct gatcgacagc tccggcgcta tcgccctgct
     8461 ggtttattta gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc gagcggaagg
     8521 aagcgaaatt cgcttgcgaa tgtggcttta tccctggctc acctggctgg tcatcggctt
     8581 tattaccttt gtgttggtag tgatgctatt ccgtccggcg caacagttag aagtgatctc
     8641 caccggctta ttagcgatag ggattatctg taccgtgcca attatggctc gctggaaaaa
     8701 gctggtattg tggcaaaaaa cacccgttca taatacgcgc tgataatgat caggagtcac
     8761 accatgacca ttacgtctct ggatggctat cgctggctga agaacgatat tattcgcggt
     8821 aattttcaac cggatgaaaa attacggatg agtttgctga catcgcgtta tgcacttggc
     8881 gttgggccgt tgcgggaagc actttctcaa ctggtggcgg aacggctggt cacggtggtg
     8941 aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg agctgctcga tattttcgat
     9001 gcccgcgcca atatggaagc gatgttagtg agtctggcga ttgcccgcgg tggcgatgag
     9061 tgggaggcag acgttctcgc aaaagcgcat ctgctcagta agctggaagc ctgtgacgcc
     9121 agcgagaaaa tgcttgatga gtgggatctg cgtcatcagg cgtttcatac agcaattgtg
     9181 gcgggctgtg gttctcacta tttgctgcaa atgcgtgaac ggttgtttga tctggcggcg
     9241 cgctatcgat ttatctggct gcggcgaacg gtgctttcgg tagaaatgct ggaggataaa
     9301 cacgatcagc accagaccct gactgcggcg gtactggcgc gagataccgc gcgcgccagt
     9361 gagttaatgc gccagcattt actgacgcca attcccatta tccagcaggc gatggctggc
     9421 aattaactac tcttccggaa tacgcaacac ttgccccgga taaattttat ccgggctttt
     9481 tagcatcggt ttattcgctt cgaagatttt attgtacaga ttagcgttac cgtagacctg
     9541 tttggaaatg gcactcagag tgtcgccaga cttaacggta taaaactggc tggcagtggc
     9601 tggtgtcgcc gttttcacct gatcatcaac actggcaata ccggaaatat tccccaccgc
     9661 aacaaggatt ttctccttcg cctcctgact caggccgtca ccagtgaccg tcgctttgcc
     9721 gtcggcaatt tgaatgttca ctttatcagc atcaggtata ccggttttgc tcagatgctc
     9781 ctgcaccttc ttcgcctgat cgtctttatc gtgctgacct gtaaccgcgt cccagagttt
     9841 ttctccggca tctttcacaa aattgaacag acccatagct acctcattcg ttaacggaaa
     9901 cactcagaaa gtgtagcaga ggaacgcggc gcgttaacgc cgcgggggtg agcacaggtt
     9961 taatcgcggg tttgcaccca gaacgcgtga atcaaaccgg ggatataccc aagcagtgtc
    10021 agcagaatat taataatgaa cgcccaaccg aaccctttac cgagcagcac gccgagcggc
    10081 ggtagaatga tggtgatgac gattctccag aaacccatat gtactcccta taagaaaatt
    10141 actcattgtt taaaaagaga ttttatctct taagcgtagt aattttagcg gagcctgcca
    10201 gtttcagctt acgcttttac tctcatttac gctgctgagg ctggtatatg ttgcattttt
    10261 agtttagagc ttacttaatt tagaaaatac ttaaataaat atgactgaac tcgcgcaatt
    10321 acaggccagt gccgaacagg cagcggcctt attgaaagca atgagccatc ctaaacgatt
    10381 gctgattctg tgcatgctta gcggttcccc cggcaccagc gcgggagagc tgacgcgcat
    10441 taccggactg agtgcctctg cgacatcaca gcatctcgct cgtatgcggg acgaagggct
    10501 tatcgacagc caacgggatg cccaacgcat tctatattcc attaaaaatg aggcggtaaa
    10561 tgccattatc gccaccctga aaaatgtcta ttgtccgtaa ggagtcacta tggctttgac
    10621 aaccatttcg ccgcatgatg cacaagaatt aatcgcacgc ggcgcaaagt taatcgatat
    10681 tcgtgacgct gatgaatatc ttcgtgaaca tattcctgaa gcagatctgg ctccattatc
    10741 cgtgctggaa cagtcaggtc ttccacctaa attacgtcgc gagcaaatta tattccactg
    10801 ccaggcaggt aaacgcacca gtaataacgc cgataaatta gcagcgattg ccgcccccgc
    10861 agaaatattt ttactcgaag atgggattga tggctggaaa agagctggat tgccagtagc
    10921 ggtaaataaa tctcaaccct tgccgttaat gcgccaggtg cagatcgctg cgggtggttt
    10981 aatattaatc ggcgttgtac tgggttatac cgtaaatagc ggtttcttct tattaagtgg
    11041 ctttgttggt gccgggttac tgtttgcagg aatcagcggt ttttgtggaa tggcaaggtt
    11101 gttagataag atgccgtgga accaacgagc ttgagaagcg acgccggacg cgccctagca
    11161 gcgacatccg gcctcagtaa ttagatcagg aaatcgtcga gagatttacc ttctgccagc
    11221 gcctgagcaa ttggcttcgg tgtacggccc tgaccggtcc aggttttagt ttcaccgtta
    11281 acatcggtga atttatattt cgccggacgc ggctggcgtt ttttaccagc gcgtggtgca
    11341 gcagcagagc tattacccaa taactcttcc gggttaattc cgtcagcctt catcagctcc
    11401 agccaggtgc taattttttc ctgacgctca gccagttcac gctgctgctg ttcttcttct
    11461 tcacgtcttt ctttagtgac aaccctgaat ttttcgagca tttcttcaag aacgtcaatg
    11521 gagaattcgc gagccatcgc acggagggtg cgaatgttat ttaaactttg taacattacg
    11581 gacataataa agaaaacctt ttaacgccaa aacaaaaaag tatttcgtcg cgtagtgtaa
    11641 gcgatcactt attatatttc cacaaaattt aatctattta cagtcttgcg cgagattatt
    11701 tcagctgtta ttttctgaaa ctctcagaat aatttcaaga ttatatatta gccattttga
    11761 tgagtaattt agattattgg tgaacagaac ggcgctctct gctcgctaat tccgcaagcc
    11821 tgacaaaacc gaagtgaata aaaatgcaat ataacagaat aaaagaa
//
LOCUS       DAJTYZ010000119        11854 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000119 DAJTYZ010000000
VERSION     DAJTYZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11854
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.119"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..201)
                     /locus_tag="OO596_003196"
     CDS             complement(<1..201)
                     /locus_tag="OO596_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCR6718982.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFD"
     gene            complement(403..1047)
                     /gene="cheZ"
                     /locus_tag="OO596_003197"
     CDS             complement(403..1047)
                     /gene="cheZ"
                     /locus_tag="OO596_003197"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6718983.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1058..1447)
                     /gene="cheY"
                     /locus_tag="OO596_003198"
     CDS             complement(1058..1447)
                     /gene="cheY"
                     /locus_tag="OO596_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6718984.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1462..2511)
                     /gene="cheB"
                     /locus_tag="OO596_003199"
     CDS             complement(1462..2511)
                     /gene="cheB"
                     /locus_tag="OO596_003199"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6718985.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2514..3374)
                     /gene="cheR"
                     /locus_tag="OO596_003200"
     CDS             complement(2514..3374)
                     /gene="cheR"
                     /locus_tag="OO596_003200"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6718986.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3393..4994)
                     /gene="tap"
                     /locus_tag="OO596_003201"
     CDS             complement(3393..4994)
                     /gene="tap"
                     /locus_tag="OO596_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6718987.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5040..6701)
                     /gene="tar"
                     /locus_tag="OO596_003202"
     CDS             complement(5040..6701)
                     /gene="tar"
                     /locus_tag="OO596_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6718988.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6846..7349)
                     /gene="cheW"
                     /locus_tag="OO596_003203"
     CDS             complement(6846..7349)
                     /gene="cheW"
                     /locus_tag="OO596_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6718989.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7370..9334)
                     /gene="cheA"
                     /locus_tag="OO596_003204"
     CDS             complement(7370..9334)
                     /gene="cheA"
                     /locus_tag="OO596_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6718990.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9339..10265)
                     /gene="motB"
                     /locus_tag="OO596_003205"
     CDS             complement(9339..10265)
                     /gene="motB"
                     /locus_tag="OO596_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6718991.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10262..11149)
                     /gene="motA"
                     /locus_tag="OO596_003206"
     CDS             complement(10262..11149)
                     /gene="motA"
                     /locus_tag="OO596_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6718992.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11275..11853)
                     /gene="flhC"
                     /locus_tag="OO596_003207"
     CDS             complement(11275..11853)
                     /gene="flhC"
                     /locus_tag="OO596_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6718993.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
BASE COUNT         2692 a         3338 c         2899 g         2925 t
ORIGIN      
        1 atcaaaatgc agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc
       61 aaaccagata acacacacgc ccactaacaa aatcagcaac gaggtcagtt cacgggaacg
      121 cgggatttgc ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt
      181 gtcgtcgctc tcgtcagaca cgtcgccaat cctggaaaga gtattaagct taaatgatgc
      241 cagagcgcaa agcgttcaat ggtttgagta aggggcaaaa caggcgggat ttagggcttt
      301 tgctgccaca catcaagcat agtgtgcgtt tgtcggatgc ggcgtcatcg ccttatcaga
      361 ccgcctgata tgacgtggtc acgccacatc aggcaataca aatcaaaatc caagactatc
      421 caacaaatcg tccacctgat cctgactggc taccacgccg actttgctgg tatcgacctg
      481 aggtccatta agcaaactct ggttttcacg ttttggacgc gactcctgtt ccgggatgtt
      541 ttccaacagc accatcagca actggcgttc gatctcctga atgacatcca tcatccgctt
      601 aatgaccagc ccggtgagat cctgaaaatc ctgcgccatc atgatttcca gcagttgcgc
      661 gttagtaaag ctggtatgcg cgggtacatc tgccagaaat tgtcgtgtat ctgttaccag
      721 ttcacgggca tcggcaaggt caatcggatc ggcaaaccag tcatcccaac gttgggttaa
      781 cgcttttgct gatttctcca tttgatcctg atgcggttgt gacgcctcaa cactgttcag
      841 cgcccgctcc gcagcctggg cggtcatctg cacaacatag tacaaacgat cgcgcgcatc
      901 ggggatggct tccgccgctt cggcaatggc ctgatccagc cccagttccc gcaaactgtc
      961 gcgcagcata cgcgtcaggc tgccgatgcg cgcaatgata tcgccagctg aatgctcgtc
     1021 agcaggtttg attgatggtt gcatcatagt cgcatcctca catgcccagt ttctcaaaga
     1081 ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg cttcaccaca tagccactgg
     1141 cccccgcttg cgccgcagca atgatgttct ctttcttcgc ttctgcagtc accattaaca
     1201 ctggcaatgc cgacatcgcg ccatccgcac gaattgtttt cagcaattcc aggccatcca
     1261 tattgggcat gttccagtcg gagataacaa atccataacc gcctgcctgc aacttattga
     1321 gagcgtcgac gccatcttcc gcttcctcaa cattattgaa tcccagctct ttcagcaggt
     1381 tacgcactat gcgtcgcatg gtggaaaagt catccacaac caaaaattta agttctttat
     1441 ccgccatttc acactcctga tttaaatacg tatcgcctgt ccggcactaa tttttgccaa
     1501 catttgctgg cttacctggc taagatcgac cacttcgcag acaccaccca tattgatggc
     1561 ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt tgcgcaaggg tccatgcccc
     1621 cgcctgacgc atcgccaaca ttcccgccgc gccgtcgttg cccataccgg tcaggatcac
     1681 cccaaccgca ttacgccccg cctgtttggc gacagaatgg aacaacacat ctaccgaagg
     1741 ccgatgacgg ttaaccgccg ggccgtcgtg aattttgatt tgataatttg cgccactacg
     1801 cgccagttcc atatgccgat cgcccggcgc aatataggca tgccccggca agacacgttc
     1861 tccgtcttcg gcttctttaa ccccgatctg gcaaagctta ttaagtctgt cggcaaaaga
     1921 gcgggtgaaa ccgggcggca tatgttgggt aattaacaat gccgggctgg aaagcggcaa
     1981 cggttgcagt acgtgacgaa ttgcctcagt tccacccgtt gaagcaccaa tcgcaatcag
     2041 tttttcagaa ctcaacaacg gacccgcctt cagcgttgtc ggtgctgaca atggcttatg
     2101 tgctgcaagg ctcgcctttg ctgccgtacg caccttttca gcaatcattt cgctatacgc
     2161 cagcattcct tcgcgaatac ccagttgcgg tttggtgaca aaatctattg cccccagctc
     2221 cagcgcgcgc agcgtgactt ctgatccttt gccggtcaag gaagaaacca tcacaacggg
     2281 cattggacgc aaacgcatta atttttcgag gaaatccagt ccgtccatcc gcggcatttc
     2341 aacatccagc gtcagcacat cgggattgaa tttcttaatc aagtcacgcg cgaccagcgg
     2401 atcaggcgcg gtcgccacca tttccatgtc gctatggctg ttgatgattt ctgtcatgat
     2461 ctggcgcatc agtgccgaat catcgacaga taacaccctg attttgctca tcgttaatcc
     2521 ttacttagcg catacaccgt ctgaccacgc agcgtgaagc ggcgctcaag gtggctaaag
     2581 ttttcagagt aacccgcaaa cagcaatccg tcgggtttaa ggagcggaac aaagcggcgc
     2641 aaaatctcct gctgggtatt ttgatcgaag tagatcatga cgttacgaca gaagatcgca
     2701 tcaaacggcc ccggcacggt gtactgtttc gccagtagat tcagcggggc aaaatcaaca
     2761 tagttcgcca gctcctgacg cacgcgtacc agcccttcat gcggtcccgt ccctcgcatg
     2821 aaataccgtt gcagttgctg cggcgtcagg tttttcaact cttcatggcg ataaataccg
     2881 cttctggctt tttccagcac ttcggtgtcg atatcactgg caaacacttt ccagcgtccg
     2941 ggcgcggtgc ccaatgtgtc agccagcgtc atcgcaatgc tgtacggctc ttcgccggtc
     3001 gaagccgccg cgctccatac gcgatactcg ccagaacggc gatgtgcgtg atccgcgagc
     3061 agagggaaat ggtgtgcctc acggaaaaat gccgtcagat tcgtggtcag cgaattgata
     3121 aacgcctgcc actcaccgct gtgctgatta gattccagca agttcagata atgaccgaaa
     3181 tccgtcagtc ccagcgaacg caaacgacga accagtcggt tgtaaaccat gtcgcgttta
     3241 tggtcagcca gaacgatccc ggctcgttga tagatcaatt gacttatccg ccgaaaatgc
     3301 gcgtcggaaa gcgccaggcg ctcggtcatc tgtaacaata aagacgtttg cccacagggc
     3361 agagatgaag tcatagcgcc ttctcaatca cttcaggata ccactggcgc aatttgtaac
     3421 tgcgccgact catgtcgtgc tacttcatgt tcttcaaggg taaataccgc cacacgcgac
     3481 gaaagatggt cggcctgatt cgccagttgt tccgttgcca ccgccgcttc ttctaccagc
     3541 gaggcgttct gctgagtcac ctgatccatc tggctgacag cctgtgcaac ctgctcaatc
     3601 ccccgctgtt gttcttccga cgcagaggca atttctccca taatgtcgtt cacgcgggtc
     3661 accgaactga caatatcgat catggtcgcg gcggcgttat tcaccagttt cgaaccctgc
     3721 tggacacgat tgactgactc ttcgatcagc cctttgatct cttttgctgc ctgcgcgcta
     3781 cggctggcaa gattgcgcac ttcacctgcc actaccgcaa aaccacgccc ctgctctccg
     3841 gcgcgagccg cttccactgc cgcattcagg gccagaatat tggtctggaa agcaattccg
     3901 tcgataacgc tgataatgtc gccaattttt tgcgagctgg tggcgatctc ctgcatggtg
     3961 tgagtcatgg tactgacctg aacaccgccc gcctgcgccg ttgtcgcggc attttttgcc
     4021 agttccgacg cctgtcgtgc gttatcggcg ttttgcccta ccgtggcggt taattgctcc
     4081 atactggcgg ccgtttgtgc cagcgatgcc gcctgctgtt cggtacgact tgagagatcg
     4141 ttattgcctg cgacaatctc cgcgatacca atgtgcatct cctggcttcc cttacgcaca
     4201 tcacttaccg tcccacgcaa agcctgctgc atggtcttca gactggcaaa aatggcggtg
     4261 atctcattac gaccatatac cgcaatcgga cgcgccagat tacccgcagc aatgctgtca
     4321 aaatggctac cgataatggc cagtggttga acaatcatct tgcgcgtcca ccacagcgca
     4381 ctgctgatgt agatcgccgc aacaataatc atgctgataa acaccagtgt cgaaatctga
     4441 tagttacgct ggctttgcgc actggcggct tccagcacat ggttgatctc cagctgccag
     4501 gcctcaaagt taacgtcaaa cgcattctgt gatccctgca ccggcgcagt gaggaaatcc
     4561 gaaagttgat tactttcaag ccaggtcgcc tgatgttcga gatcgttgtg ccagcgggca
     4621 aaacttttct ccgtctcttt ttgcaatgcc ctgacgtgct cgttgcccgc agtcatcgcc
     4681 ataaaacttt taaacagcgt ggtggattgc gtcagactgg cgcgcgccgt cgtcatcaac
     4741 gttttaatgt catccgccgg atagctaagt gccgtcagag tgcccgcttt gttcagcgcg
     4801 gtactggcct gtaacattac cgcccgagtt tgcgctaatg ccgcacgttg ctgattgctc
     4861 tgctcgacct gattcagtcg ttgcaaatcg tcgcgaaatg cccaaaaaga catgccgtta
     4921 ctgccaatct gcaagatccc gcagagaatc aaaattaaaa acagcgtggt cgaaattcga
     4981 atacgattaa acatcaacgc tccccatcag gcggcaatga ccgcgttagt aaatactcgt
     5041 caaaatgttt cccagtttgg atcttgttca gcaattcgca gtcgtggctg tgccggtggt
     5101 tgctcactgg caggacggga tggtgtttgc ggtttattgg tgagtgggct ggctgccaga
     5161 cggaacgcgg aaaccgcttg cgttaaacga ctcgcctgtt cttccagcgc agcggcggcg
     5221 gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga cgcgatccat ttccgaaacc
     5281 gccaatgcga cttgatcgat gccacggctc tgttcatccg atgccgatgc aatctcgccc
     5341 ataatgtcag tcacgcgagt gacagcattg acgatattgt tcattgtttc cccggcgctt
     5401 tcgaccagca ccgaaccggt atcaacgcgt gcgacggagt cttcaatgag ggctttgatc
     5461 tcttttgccg cctgggcgct gcgactggca agattacgca cttcacccgc caccacggca
     5521 aaaccacggc cctgttcacc cgcacgcgcg gcttcaaccg cggcattcag cgcgaggata
     5581 ttagtctgga aggcaatacc gtcgataacg ctgataatgt cggcaatttt cttcgaacta
     5641 tcggcgatct catgcatcgt tttcactacg ccatccacca ctttgccgcc gtgctgggcg
     5701 gtgtcggagg cactttgcgc cagttgcgag gcctggcggg cgttatcggc gttttgcttc
     5761 actgtcgcgg tgagctgctc catgctggcg gcagtttctt ccagcgcgga tgcctgctgt
     5821 tcagtacggg aggaaagatc ggtgttgccc gccgcaattt cacgggtacc ggcatagatg
     5881 gcatctgaac cttcgcggac atgagtgacg gtgtcagtca aagagcgttg catatgtgaa
     5941 acgctctgcg ccaggtcgcc catttcactg cgcccgtcaa tggtcagggt attcgctagg
     6001 ttaccaccgg cgatttcgcg aatgtgagca ataatttttg ccagcggagt aagcaacata
     6061 cggcgaatgc cgtaccacgc caccagcaga atcaatacca ccaccagcgc gataaccgcc
     6121 agttgccact gggcaaatcg gtaatcatct gcgttgtcag tgacgatatc gcgatacagt
     6181 ttttcactgc tgagggcgta ctgagcaaac gcttcgccca ttgcattttg cattccctgg
     6241 gttggctgag cgaaataagc tccagtattg ccataatcta gataatcaat cagttcagtt
     6301 aacgctgtgt aatagttttt atatttttca tcaatattac gactggtagc gaccatttca
     6361 ggtaacggtg ccatgctttt gaatttttta taatgcgtcg ctgcctgcgc caatgttttc
     6421 ctggcgctat cgagcaattc aactttggcg ttactttgtt gattggagga atccatcatc
     6481 atccgcaccg ctgaacgact caggttaatg cgcgtttgca gcattaaatc ccaggttgac
     6541 gtcagctcgc cctgctgttc ccgcaattga ttggaaacca caaagctctt ctggctatgg
     6601 tgaagggaag aaaaaaacag actgccggaa ataagctgta acagtgcgaa tacccccagc
     6661 accattacca acagcgtgac tacgcggata cggttaatca taaggcacct tcctgaaaac
     6721 aagttgatct cgttatcggc aagaaggggg gaaactttat tgctgatgcc acccgccgcg
     6781 aaattgaaat aaaaaacccg atgcgcagat catcgggttc atttcaattg aggaaatcgg
     6841 gagaattacg ccacttctga cgccgcgcta tctaacagcg ccatctcttc gctgttcagc
     6901 agtttttcga tgttcaccag aatcaacata cggtcgccca gtgcgcccag tccagtgaga
     6961 tattctgttg aaagcgtcac ggcaaactct ggtgccggac gaatttgctc cgccgtcaat
     7021 gaaagcacgt ctgaaacgcc gtcaaccacg atgccgacca cccgctgtcc gagattcagg
     7081 acgataacta ccgtgttgtc gttatagtcc acatccacct ggctgaactt aattcgtaag
     7141 tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa cgctggcgtg
     7201 ttcgcaatcc gtgttacctg atcgtagcca cggatctcct gcactttcag gatatcaata
     7261 ccgtactctt catcaccaag ggtaaatacc agaaattcct ggcctgacgg ctcgctggcc
     7321 agctttgtta cattcgtcat accggtcata ttgttacctt tttactcatt caggcggcgg
     7381 tgttcgccat acgttgttcg cggtttatcg cctgcaaggc ggagacatca acaatcagtg
     7441 ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc ggggactttg cgatagttac
     7501 tttcaaggtt tttaaccaca acctggtgtt gaccaattaa ttgatccacc agcaaggcgt
     7561 agcggcgacc gccactttgt aagatcacaa caattccctg ggtggcttcg gttttcgcgc
     7621 ccgcgacgtt gaacactttc cacagttcga cgatgggcag atattcaccc cgcacttcca
     7681 gtacccgctc gccgccggcc agtggatgga gatcggcttc acggggttgc agtgattcca
     7741 taacagcatt cagcggcaga atgaaaactt catccccaac gcgtacggac atgccgtcga
     7801 ggatggccag cgtcagcggc agtaaaatgc ggatcgtagt gccagtcccc tgcttcgact
     7861 ggatttcgac atgaccgccc atctcctgga tattacgttt aacgacgtcc atgccgacgc
     7921 cgcgcccgga gacgtcggtg acctgctctg ccatggagaa gcctggtgca aatatcagca
     7981 tcgcgacttc gtcgtcgctc atgttttcgc tgacagtcaa accttgcgag gccgcttttg
     8041 ccagaattcg ctcacggttt agccccgccc catcgtcggt cacttcaatg caaatgttgc
     8101 cgccctgatg ttcggcagac agaattaaat ttccgacgct gtttttacct gcggcgagcc
     8161 gtttttctgg cagttcaata ccgtgatcga ggctattgcg taccaggtgg gtcagcgggt
     8221 cgataatgcg ttctatcagg cttttgtcga gttcagtaga actgcccacc agcgtcagtt
     8281 ctacctgctt gccgagtttt cccgccagat cacgcaccag ccggggatag cgactaaaaa
     8341 catattccat cggcatcatg cgaatcgaca tcactgattc ctgcaaatca cgggcgttac
     8401 gttgtaactg ccccatgctg gttatcaaat caccatgatt aaccgggtcc agttcgctgg
     8461 aacgctgggc aagcatggac tgggtgataa ccagctcgcc gacgaggtta attaattgat
     8521 caaccttttc taccgctaca cggatgctgg tggattcatt gctgcgcgtc gttttttccc
     8581 gctccacgcg gccggttggc gcttgttcgg ctgccagttt aagcactggt ggggtggata
     8641 tttttggcga gacttctact gtttcaaagg taatctgatc ggcttcaatc acaaaacaga
     8701 gtaccgctgt gatgtcatct tcggcgatgt cgcccggtaa tattgccgag agcgaatccg
     8761 cccctttcac cacgtcagtt aacgttgtca gatgtcccag ttcttcttcc agcaggtcga
     8821 cttccccggc cttcaggcgc gaaaggataa ttcgtcgcgg cgactgactg cgactctgct
     8881 catcttgcgg ttcacttttg gcaaccacac ttaatcgggt cactgcggat ggcgtttcgc
     8941 ctttcgcttc taatgccagt tgacgcaagg cctggcagat ataatcgaag ctggcggcat
     9001 ccggctcttg cgactgttta taagcgtcga gctgttcttg catgatgtcc ttcgtttcca
     9061 aaaacagatt gataatgtcg gtgttgagtt gcatctcacc tcgtctggct tcatcgagca
     9121 ggttttccat cagatgcgtg gtttcctgca aaacgctgaa gccaaaagtt cctgcccctc
     9181 ctttgatcga gtgggcagcc cgaaagatgg cattcaattg ttcggcatct ggcgcttccg
     9241 gctgcaaaac cagcagatgc tgctccatgt cagccaacag ttcgtccgct tcatcaaaaa
     9301 atgtctgata aaaatcgctt atatccatgc tcacgctgtc acctcggttc ggctgatggc
     9361 attgtgggaa cactgacctg tggtgcaacc tcaggttttt ccagggcgct tactggctca
     9421 ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg cttgtttgtt cagtaccagc
     9481 aggctgatgc gacggttgac ggcatcatca ggtccgcgat cgcttaagcg catcgttgcc
     9541 gccatgccga cgacacgtaa cactttgccg ctatccaacc ctccgaccat cagttcgcgg
     9601 cgggatgcat tggcccgatc ggcagaaagc tcccagttgc tatatccttt ctcaccgctg
     9661 gcgtagggga aatcatcggt atgacctgaa aggctaatac ggttgggaat accgttcagt
     9721 acaggcgcaa tgacacgcag aatgtcgcgc atatagggtt cgacatcggc actgccggtt
     9781 ctaaacatcg ggcgattctg gctatcgatg atctgaatac gtagaccttc ctggaccaga
     9841 tcgattttga gatggggacg taacgcccgc agtttcggat cggactctat caactggtcg
     9901 agatcacccc gcaatttccg caatcgactt tgctccatgc gttttttcag atcttcgatg
     9961 ttcggctgct tattcacttc cccctggctt tgggtgtaat catcaccacc gccgggaatt
    10021 gggctttcac tattagaaat gcgatcgccg cccgtaaccg cagtcgccag tggagtccgg
    10081 aagtactccg caatctgaat cagctctttt gggctggaga tggagatcag ccacatcacc
    10141 agaaaaaagg ccatcatcgc agtcataaag tcggcataag caatcttcca cgatccatgt
    10201 gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg cgcttgattc
    10261 ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt caccgcacgc acatgctctt
    10321 ccagttcaat aaacgacgga cgttcgctgg aatagagcgt tttgcgacca aactcaacgg
    10381 cgataggcgg tgcgtaaccg ttcagattag aaagcagagt gactttgacg cactgcatca
    10441 ttttgctggt ttcggcgctt ttctgacgta aaacactcgc taatggggaa ataaatccgt
    10501 aagccaataa aatgccgagg aaagtcccca ccatcgcatg tgcgataagc gcacccagct
    10561 cggcggcagg acgatcggct gaacctaacg cgtgaacgcc ccccattaca gccgcgacaa
    10621 taccaaacgc cggaagtgag tccccgacca gcgccagact gttcgccggg acttctgcct
    10681 cgctttcgtg cgtctcaatc tcttcatcca tcagggcttc gatttcgaag gtgttcatat
    10741 gaccgctgat aatcagacgc agataatcga cgataaaatc aagcatgacg ctatccgcga
    10801 ggatgcgtgg gtagctggcg aagatctcgc tctcacgggg attttcaata tcacgttcca
    10861 gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga gccagcagat
    10921 ccatatacat tgctttggtg tatttggagc gacgaaacag caacggcagc gccttcagcg
    10981 tgcctttaat cgctttgcca ttattgccga cgataaacga cccaatccct gcaccggcaa
    11041 taatcaccag ttcagcgggt tgatagagtg ctccaaggct tccaccggtc atcaaataac
    11101 cgccgaaaac tgtaccgaga acaaccaggt aacctaataa gataagcacg acatcatcct
    11161 tccactgttg accatgacag gatgttcagt cgtcaggcgt taacgcgcga ttggggcaaa
    11221 aaaagcagcg gtacgtcgtt accgctactg gaatgttgcg cctcaccgta tcagttaaac
    11281 agcctgtact ctctgttcat ccagcagttg tgggataata tcggcaggat tctgggaaag
    11341 tttacgtctt tttactgccc gggatggcgg ttgacataag ctgcaggcaa agctgccaac
    11401 aggctggtga gcgtgggtaa taaaattgcc gccgcagcag ttgcagctgg aaagttgcag
    11461 taatccactt tcaacaaacc gcaccaatgt ccaggcacgg gttaatgcca gcagtggtcc
    11521 ttcttctgct tgtgggcact gttcaaggta taaacggtag gctttgatca ccgcatcgac
    11581 gccattacac aaaccggttt tcagtaaaaa ctgccatgca ttacagaaca tcgaagcatg
    11641 aacgttttgt tcccaggtca taaaccagtc ggttgagaat ggcagcatgc ctttcggcgg
    11701 tgggcttccg cgcagttctt tataaagttt tatcaggcgt ccgcgactta actgtgtttc
    11761 gctttccagc atctgcaaac gagcgcccag ggtaatcaat tccattgcca gctgaatatc
    11821 ccgcgcttcc tgaacaatgc ttttttcact catg
//
LOCUS       DAJTYZ010000120        11656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000120 DAJTYZ010000000
VERSION     DAJTYZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.120"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            76..1153
                     /gene="ygjJ"
                     /locus_tag="OO596_003208"
                     /pseudo
     CDS             76..1153
                     /gene="ygjJ"
                     /locus_tag="OO596_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1170..3521
                     /gene="ygjK"
                     /locus_tag="OO596_003209"
     CDS             1170..3521
                     /gene="ygjK"
                     /locus_tag="OO596_003209"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6718994.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            3747..5765
                     /gene="fadH"
                     /locus_tag="OO596_003210"
     CDS             3747..5765
                     /gene="fadH"
                     /locus_tag="OO596_003210"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6718995.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(5810..6226)
                     /gene="higA"
                     /locus_tag="OO596_003211"
     CDS             complement(5810..6226)
                     /gene="higA"
                     /locus_tag="OO596_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6718996.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6223..6537)
                     /gene="higB"
                     /locus_tag="OO596_003212"
     CDS             complement(6223..6537)
                     /gene="higB"
                     /locus_tag="OO596_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6718997.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(6822..7958)
                     /gene="rlmG"
                     /locus_tag="OO596_003213"
     CDS             complement(6822..7958)
                     /gene="rlmG"
                     /locus_tag="OO596_003213"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6718998.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8043..8546
                     /locus_tag="OO596_003214"
     CDS             8043..8546
                     /locus_tag="OO596_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6718999.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            8623..9306
                     /locus_tag="OO596_003215"
     CDS             8623..9306
                     /locus_tag="OO596_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6719000.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9385..10371
                     /locus_tag="OO596_003216"
     CDS             9385..10371
                     /locus_tag="OO596_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6719001.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            10654..11619
                     /gene="alx"
                     /locus_tag="OO596_003217"
     CDS             10654..11619
                     /gene="alx"
                     /locus_tag="OO596_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6719002.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2768 a         3007 c         3135 g         2746 t
ORIGIN      
        1 tagtttctgc ctttatattt ttataattac aacgataaaa ggctgtactt tttctttagc
       61 tcatggatta acacaatgaa attaatcact gcaccatgca gagcattact tgctctgccg
      121 ttttgctacg ccttttctgc ggcaggagaa gaagcacgtc cggcagaaca tgacgacaca
      181 aaaacacccg caattacctc gacatcttct ccttcatttc gtttttacgg cgaattaggg
      241 attagggggt ggtggatata tggatttaga gggtgagaat aaacataaat acagcgacgg
      301 tacctatatt gaaggtggcc tggagatgaa gtacggctcc tggttcggcc tgatttacgg
      361 cgaaggctgg accgtgcagg ccgaccacga cggcaatgcc tgggtgccag accatagctg
      421 gggtggtttc gagggcggaa ttaaccgttt ctatggcggt tatcgtacca atgatggcac
      481 cgaaatcatg ctcagtctgc gtcaggattc ctcgctggat gacctgcaat ggtggggcga
      541 tttcaccccc gatctgggct acgtcattcc caatacccgc gacattatga ctgcgctgaa
      601 ggtacagaac ttaagcggca actttcgtta tagcgtcacc gcgactcctg ccggacatca
      661 tgatgaaagc aaagcctggc tacattttgg caaatacgat cgctatgacg acaaatacac
      721 ctatccggca atgatgaacg gttacatcca gtatgacctt gccgaaggca tcacctggat
      781 gaacggtctg gaaatcaccg acggcacagg acagctctat ctcacgggcc tgctaactcc
      841 taactttgcc gctcgcgcct ggcaccatac cggacgcgcc gacgggctgg acgtaccggg
      901 aagtgaaagt gggatgatgg tgagcgccat gtatgaagcg ttaaagggcg tttatctctc
      961 caccgcttac acctacgcca aacatcgccc tgaccacgct gacgatgaaa ccacctcttt
     1021 catgcagttt ggtatctggt acgaatacgg cggcggacgt ttcgccacgg cttttgatag
     1081 ccgcttctac atgaaaaatg cctctcacga tcccagcgac caaatcttcc tgatgcaata
     1141 tttctactgg taataaggac tgtaatttca tgaaaataaa aactatttta acgccagtaa
     1201 cctgcgctct gctgataagt ttttccgccc atgccgctaa cgccgacaat tataaaaacg
     1261 tgattaaccg tactggcgcg ccgcagtaca tgaaggatta cgattacgac gatcaccagc
     1321 gttttaatcc gtttttcgat ctcggagcct ggcatggtca tctgttgcca gacggcccta
     1381 acaccatggg cggctttccg ggcgttgcgc tgctgacgga agagtacatc aactttatgg
     1441 ccagcaattt cgaccgcctg accgtctggc aggacggcaa gaaagtcgac tttacgctgg
     1501 aggcatacag tattcccggt gcgctggtgc aaaaactgac agcaaaagat gtgcaagtcg
     1561 aaatgactct gcgcttcgcc acgccgcgca cgtcactact ggaaaccaaa atcaccagca
     1621 ataaaccgct ggatctggtg tgggacggcg aactgctgga aaaactggaa gcgaaagaag
     1681 ggaaaccgct ttccgataaa accattgctg gcgaataccc tgactatcag cgcaaaatca
     1741 gcgccacccg tgatggcctg aaagtcacct ttggcaaagt gcgcgccacc tgggatctgc
     1801 tgacctccgg cgaatcggaa tatcaggtgc ataaatccct gccggtgcag actgaaatca
     1861 acggtaatcg ctttaccagt aaggcgcata tcaacggttc gaccacgctc tacaccacct
     1921 attcccatct gctgaccgct caggaagtta gcaaagagca aatgcagatc cgcgatattc
     1981 tggcgcgtcc ggcgttttat ctcaccgcct cgcagcaacg ctgggaagaa tacctgaaga
     2041 aagggttaac caatccggat gcgacgccgg aacagacgcg cgtcgcggtg aaagcgatcg
     2101 aaacgctcaa cggtaactgg ctctcaccgg gcggtgcggt gaaatttaac accgttacac
     2161 cgtcggtgac cgggcgctgg ttctccggca atcagacctg gccgtgggat acctggaagc
     2221 aggcgtttgc gatggcgcat ttcaatccgg acatcgccaa agagaatatc cgcgcggtct
     2281 tctcctggca gatccagcct ggcgatagcg tgcgtccgca ggatgtgggc tttgtccccg
     2341 acctgatagc gtggaacctt agccccgagc gtggtggcga tggcggcaac tggaacgaac
     2401 gtaataccaa acccagcctt gccgcctggt cggtgatgga agtgtacaac gtcacccagg
     2461 ataaagcctg gttggcagag atgtacccga aactggtggc ctatcacgac tggtggttac
     2521 gtaaccgcga tcacaacggc aacggcgtgc cggaatatgg cgcgacccgc gacaaagccc
     2581 acaacactga gagcggcgag atgctgttta cggtgaaaaa aggcgataaa gaagagacgc
     2641 agtctgggct gaacaactac gcccgcgtgg tggagaaagg cctgtacgac agtctggaaa
     2701 ttccggcaca ggttgctgca tcgtgggaat cggggcgtga tgatgccgcc gtctttgggt
     2761 ttatcgacaa agaacagctg gataaatatg tcgctaatgg cggcaaacgt agcgactgga
     2821 cggtgaaatt cgccgaaaac cgcagtcagg acggaacgtt gctgggctac tcgctattgc
     2881 aggagtcggt ggatcaggcc agctatatgt acagcgataa ccattatctg gcggagatgg
     2941 caaccatcct cggtaagcca gaagaggcca aacgctatcg ccagttggca cagcagctcg
     3001 cggactacat caacacctgt atgttcgacc cgactaccca gttctactat gacgtacgta
     3061 ttgaagataa accactggcg aacggctgcg cgggcaaacc gattgttgag cgcggtaaag
     3121 ggccggaagg ctggttgccg ctgtttaacg gtgcggcaac gcaggccaac gccgacgcgg
     3181 tggtgaaggt gatgctcgat cctaaagagt tcaacacctt tgtcccgctg ggaacggcgg
     3241 cgttaaccaa tccggctttt ggcgctgata tctactggcg tgggcgcgta tgggtggatc
     3301 agttctggtt tggtctgaaa gggatggagc gttacggtta tcgtgatgat gccctgaagc
     3361 tggcggatac gttcttccgg cacgccaaag gattaaccgc cgatggccca attcaggaga
     3421 attacaaccc gctgactggc gcacagcaag gcgcaccaaa tttctcctgg agtgccgctc
     3481 atttgtatat gttgtataac gattttttcc gtaagcagta atttgcaacg gctggcggat
     3541 gcggcgtgaa cgccttatcc gccctacatg tgcaatcccc caggccggat aaaacgcggc
     3601 aagcgttgca tccggcgtta ccaccgggcg tattcttttt gaatcccatc acaaaccccg
     3661 cactcccctt ttcccttttc tccatcgacg gctaaattag aactcatccg accacataac
     3721 aattatttta catactggac aattttatga gctatccgtc gctgttcgcc ccgctggatt
     3781 taggttttac cacgttaaaa aaccgcgtgt tgatgggctc aatgcacacc gggctggagg
     3841 aatacccgga cggtgccgag cggctggcag cgttttatgc cgaacgcgcc cgtcacggcg
     3901 tggcgctgat tgtcagcggc ggcattgcac cagatttaac aggcgttggc atggaaggtg
     3961 gcgcaatgct caacgacgcc agccagatcc cacaccatcg caccattacc gaagcggtac
     4021 atcaggaagg cggcaaaata gccctgcaaa ttttgcatac cgggcgctac agctaccaac
     4081 cgcatctggt cgctccctcc gcattgcagg cccccatcaa ccgtttcgtt cctcacgaac
     4141 tcacccatga agagatcctg caactgatcg acgatttcgc ccactgcgcg caactggcac
     4201 gggaggcagg atacgacggt gtagaggtaa tggggtccga agggtatttg atcaacgaat
     4261 ttctgacgct gcgcaccaat cagcgtagtg accagtgggg cggcgattac cgcaaccgga
     4321 tgcgatttgc cgtagaagta gtgcgtgcgg tgcgcgaacg cgtcggcaac gacttcatta
     4381 ttatctaccg actgtcgatg ctcgacctgg tcgaagacgg cgggactttt gccgaaacgg
     4441 tagagctggc gcaggccatt gaagcggcgg gcgcgaccat tatcaacacc ggcattggct
     4501 ggcatgaagc acgtattccg accattgcca cgcccgtgcc gcgcggcgca tttagctggg
     4561 tcacgcgcaa attgaaaggc cacgtctcgc tgccgctggt aaccaccaac cggattaacg
     4621 atccgcaggt tgccgacgat attctctcgc gcggcgatgc cgatatggta tcgatggcac
     4681 gaccgtttct tgctgatgcg gaactgctgt caaaagcgca atcggggcga gccgatgaga
     4741 tcaacacctg cattggctgc aatcaggcct gcctcgatca gatcttcgtt ggcaaagtca
     4801 cctcgtgcct ggtgaatcct cgcgcctgcc acgaaaccaa aatgccaatc cttcctgccg
     4861 tgcagaaaaa aaatctggcg gtggtcggtg cgggacctgc tgggctggcg tttgccatta
     4921 acgcggcggc gcgtgggcat caggtaacat tgtttgatgc ccacagcgag atcggcgggc
     4981 agtttaatat cgccaaacag atccccggca aagaggagtt ttacgaaacg ctgcgctatt
     5041 accaccggat gatcgaagtg acgggcgtga cgctgaaact caatcacacc gtgacggcgg
     5101 atcagttaca ggcgttcgat gaaacgatcc tcgccagtgg gatcgtgccg cgcgttccgc
     5161 ctatcgacgg gatcgatcat ccgaaggtat tgagttatct cgatgtactg cgcgacaaag
     5221 cgccggtagg caacaaggtt gccatcatcg gttgtggcgg gattggtttt gatacggcga
     5281 tgtatttaag tcagccgggc gaatccacca gccagaatat cgccaggttc tgtaatgagt
     5341 gggggatcga cagcagccta caacaggctg gtggcttaag cccgcaggga atgcagatcc
     5401 cgcgtagccc acggcaaatt gtgatgctcc agcgcaaagc cagcaaaccg ggacaggggt
     5461 taggcaaaac caccggctgg atccatcgca ccaccctgct ctcgcggggt gtgaaaatga
     5521 tcccaggcgt aagttatcag aagattgacg atgacgggct gcatgtggtg atcaacggcg
     5581 aaacgcaggt attagcagtg gacaatgtgg tgatctgcgc agggcaagag ccaaaccgcg
     5641 cgctggcgca accgctgatt gatagcggga aaactgtgca tttaattggc ggctgcgatg
     5701 tggctatgga gctggacgca cgacgggcaa ttgcccaggg aacacggctg gcgctggaga
     5761 tttaaatcgt tattttaagc gccggatgca acgcatccgg cacgtcgcgt taatcaataa
     5821 acaaggcggg agaaatgccg aatcgcgttg ccaatttttt agcgtgttcc agcgttaatt
     5881 tcctcttccc gctcaaaacg cgtgacacca tagatttact gccaatttcc ggcagatcgg
     5941 caagggttaa accatattga tccataaggg tacgaatcac ggctataccg ccaggcatgg
     6001 cttgagccat ggcattaaat tccgcaaatt cgggcgctga ttcttcccac gcggttattt
     6061 tggcacacac cagatccagc aaggggtttt caggatcgtt gagcagcaga tgatctacca
     6121 gttccagcgc ctgggtgtat tgttcctcgt tctgaatacc cgccagaaaa ggtgccaccg
     6181 cagttagctt ttctcctgct tgcaagatgt cggcaatcgc aatcattttt tccccttagt
     6241 acgatgaaca gcggtaaaga aatcgtattc tttatgcgtc ataacttcac gtatgtagca
     6301 cttttgcgat tcaaaaaaga ccattgctac aacacgtaat tcattgcccc caacattgaa
     6361 aacataatgc ttatccagat atttgaagtt atccagagat gggaatactg cttttaatga
     6421 ctcaggtttt ttgaaatatc ccttagcaat cgtgttcccc agagccacca actccgtttt
     6481 atgttgcggg tatttttccg cagcatcttt caatgctttt tgagttatca ggtgcattct
     6541 tcatcacgtc cgttgacaaa ttggcaatat gataacatcc gttgacagat tggcaacgga
     6601 tgaatttatc tgtgtgacgg aagaataaac gtagaaaaac gctaaacaag agaatgtaga
     6661 ggatggtgag agggattttt tgttttatgg aagcgcgatt ccagaactgg gcagacattg
     6721 ccggatgcga cgcttgccgc gtcttatccg gcctgggagt aacgtgctga acgtaggccg
     6781 gataaggcgt taacgccgca tccggcaggt aactcccaat cttaccgacg acgccccagc
     6841 ttcactgctt tcagcaccac aaatttatta ttcgtggcga tggtggtgca gttgccgaaa
     6901 atcttcttca gtttatggaa gtaatccagg tgacggttgg caacaatata cagctcgccg
     6961 ttgattttca ggcagcggcg ggcatggtgg aacatctccc aggcgacgtt atcggtcagc
     7021 gcatgttgtt ggtgaaacgg cgggttgcag agcacagcat taaagcggaa aggctccacg
     7081 ccggagagcg cgttgttgat cataaactcg ctgcgatcta acgcctctgg catgttggtt
     7141 tcaacgttca aacggctgga agcaaccgcc atcggcgatt catcgacaaa caccactttc
     7201 gcctgagggt ttttatcaag cagcgtcaga ccaataacgc cattaccgca accgagatcg
     7261 acaatctcac cttcgagatt ctctggcaga tgttgcataa agaagcgtgc gccaatatcc
     7321 agcccggtgc gggagaagac attcgcatgg ttgtggatag tccagtcagt accttccagc
     7381 ttccagctaa cggtctgcgg cgcatcggcc agcggcggtt cattgaaagt gcaattaatc
     7441 aggcgcgctt ttttccatgc cagcgtagtg gtggtcgggc cgagcacttt ctcgaacagc
     7501 tccagcgtgg aagtgtgaat gtcacgggct ttggcaccgg caataatacg tgtatccgac
     7561 gtgaccactt tgcgcagcgc acgcagttgc tgttccagca atgccagtgt tttcggcact
     7621 ttgatcagca ccacacccgg ctgttgcggg tagtcggcgg tgctgtcgag aaacttcacg
     7681 ctcgactcat caatcccgtt gaggcgtaaa ttctcgcgcg tcgccagttc actgatgtat
     7741 gagtcgccaa tgctgtacgg cttatgttcc gccagcgcgc agcttaacgc accaaaggca
     7801 tcattcagga tcaacaccgg gccgcggatt tctgtgtcgt ccaactgttg cagcaaatat
     7861 tcatccgccg cttcccacgc ctgtagcggg ttaacgtcat ccgtcgccgg aaaacgttgt
     7921 agtgtcagtg aacggaaacc gttgtctaag tggctcatcg gccctcctga atgataaaat
     7981 ttcgacgttg cccctgaaaa gggtgcgtga gtataccctt tttctctttt tcgtgggcgg
     8041 ttatgagcaa tcttacttat cttcagggtt atcccgagca gctactttcc caggtgcgaa
     8101 cgctgattaa cgaacagcgt ctgggcgatg tgctggcaaa acgctatccg ggaacacacg
     8161 actacgccac cgataaagcc ctctggcaat atacccagga tctaaaaaat cagtttctgc
     8221 gcaatgcccc gccgatcact aaagtgatgt atgacaataa gatccacgta ctgaaaaacg
     8281 cgctcggatt acataccgct gtttctcgtg tgcagggcgg caagctgaaa gcaaaagcag
     8341 agatccgcgt cgctactgtg tttcgcaacg cgccggaacc gtttttacgc atgatcgtgg
     8401 tgcacgaact ggcgcacctg aaggagaaag agcataacaa agcgttttac cagttgtgct
     8461 gccatatgga gccgcagtac caccagcttg agttcgacac ccggctgtgg ctaacgcagt
     8521 tgtcgcttgg gcaggacaaa atctgaaaat gatgtcaggc gttatctttt ctttctttag
     8581 cgcctaaaat cgacctcccc cctttcgtta aggataacga ccatgcagcc ccgccttctt
     8641 ctccgtattt gtttttctcg ccggactcta aagattacct gcttactgtt acttgtggca
     8701 ggagcgacca tttttatcgc cgatcgcgta atggtcaacg ccagcaagca gctgacctgg
     8761 agcgatgtca acgcagttcc ggcgcgtaac gtgggtttat tgctgggggc gaggccgggt
     8821 aatcgctact ttacccgacg tattgatacc gcagcagcgt tgtaccacgt cggaaaagtg
     8881 aaatggctgt tagtcagtgg cgataacgga cgtaaaaatt acgatgaagc ctcaggtatg
     8941 cagcaggctt tgatcgctaa aggcgtaccg gcgaaagtga tcttctgcga ctatgccgga
     9001 ttctcaacgc ttgattcagt ggtgcgtgcc aaaaaggtgt ttggggaaaa ccatatcacc
     9061 attatctcgc aagaatttca taaccaacgc gccatctggc tggcaaaaca gtacggcatc
     9121 gacgctatcg ggtttaacgc gccagacctt aatatgaagc acggttttta tactcaactg
     9181 cgggagaaac tcgctcgtgt cagcgcggtg attgacgcca aaatcctcca tcgccagccg
     9241 aaatatctcg ggccatcggt catgattggg ccgtttagtg agcatggctg tccggcgaaa
     9301 gaataatgcg tatctgcgca cgtcgaagat gaaaaaggcg tgctacattg acgacagaat
     9361 ccctttatgg agtatccacg cgttatgata cgtttcgctg tgattggtac gaactggatc
     9421 actcgccagt tcgtcgaggc cgcccatgag agcggtaaat acaagttaac cgccgtatat
     9481 tcccgcagcc ttgaacaggc ccagcacttc gccaatgatt tttctgtcga gcatctgttt
     9541 acctcgctgg aagcgatggc ggaaagcgat gccattgacg cggtgtatat tgccagcccg
     9601 aatgccctgc acttttccca gacacaactt ttccttagcc ataaaattca tgtgatttgc
     9661 gagaaaccac tggcgtcgaa tctggcggaa gtggatgccg ccattgcctg tgcgcgggaa
     9721 aatcaggtgg tgctgtttga ggcatttaaa accgccagcc tgccgaactt tcatttgctg
     9781 cgccaggcgc tgccgaaagt cggcaaattg cgtaaagtct ttttcaacta ttgccagtat
     9841 tcctcgcggt atcaacgtta cctggatggt gagaatccca acacctttaa tccggcattc
     9901 tctaacggtt caattatgga tatcggcttt tactgcctgg cgtcggcggt ggcattattc
     9961 ggtgaaccga aaagcgtgca ggcaaccgcc agtttgttgg caagcggcgt agatgcccac
    10021 ggcgtggtgg tgatggatta cggtgatttc agcgttacgt tgcagcactc aaaggtcagt
    10081 gattctgtcc tggaaagcga gattcagggc gaagcgggat cgctggtgat tgaaaaactg
    10141 tctgaatgcc agaaagtgtg cttcgtgccg cgtggcagcc agatgcagga tctcacccag
    10201 ccgcagcata ttaataccat gctctacgaa gcagagctgt ttgctaccct ggtggatgag
    10261 catctggtgg atcatccagg gctggcggtc agtcgcatca ccgccaaact gctgactgag
    10321 atccgccgcc agactggggt gatttttccg gcagatagcg taaaactata attgccaaag
    10381 taaaacagtg taaaaggtat gtaacagacc attgactggc tgaatggtct gtcatacttt
    10441 gttacctgca aaggggagta acttcattgc cggtcgatcg tcattacgat gtgtgaaaaa
    10501 ccacatccgg tcaccgggca acccgaaagg aatacgcaga cgtattcctt ttttgttgta
    10561 agtgagacct tgccggaagg cgaggtctat gcataaaaag cagcggctga cgtcttccga
    10621 cgttggccgt ttttttatgt gtaaggaact tctatgaata ctgtcggcac gccgttgcta
    10681 tggggcggat tcgctgttgt tgtcgccatt atgctggcta tcgacctgtt gttgcagggg
    10741 cgtcgtgggg cacatgccat gaccatgaaa caggctgcgg cctggtcgct ggtctgggtg
    10801 acgctgtcgt tactgtttaa cgccgctttc tggtggtatc tggtgcaaac tgaaggtcgc
    10861 gccgttgccg atccacaggc actggccttt ctcacgggtt atctgattga gaaatcgctg
    10921 gcggtcgata acgtctttgt ctggctgatg ttgttcagct atttctctgt tccggcggca
    10981 ttacaacgcc gcgtgctggt gtatggcgtg ctcggggcga ttgttctgcg taccatcatg
    11041 atcttcactg gcagctggct aatttcgcag ttcgactgga tcctgtatat cttcggtgcc
    11101 ttcctgctgt ttaccggcgt gaagatggcg ctcgcccatg aagatgaatc aggcattggc
    11161 gacaagccgc tggtgcgctg gctacgcggt catttgcgca tgaccgacac catcgacaac
    11221 gagcatttct ttgtgcgtaa gaatggattg ctgtacgcca caccgctgat gctggtactg
    11281 attctggtgg aattgagcga cgtgattttc gccgtggata gcattccggc tatcttcgcc
    11341 gtgaccactg acccgttcat tgtgctgacc tcaaacctgt ttgcgatcct cggcctgcgt
    11401 gcgatgtatt tcctgctggc gggcgtagca gagcgtttct cgatgctcaa atatggcctg
    11461 gcggtgattc tggtgtttat cggtatcaag atgctgattg tcgacttcta ccatattcca
    11521 atcgccgtct cgctgggcgt ggtgtttggc attctggtga tgacgtttat tatcaacgcc
    11581 tgggtgaatt atcggcatga taagcagcgg ggagaatgat tcgctgtata ttaagccatt
    11641 cctagagata actcat
//
LOCUS       DAJTYZ010000121        11542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000121 DAJTYZ010000000
VERSION     DAJTYZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.121"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            460..963
                     /locus_tag="OO596_003218"
     CDS             460..963
                     /locus_tag="OO596_003218"
                     /inference="COORDINATES: protein motif:HMM:NF026057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6719003.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKET"
     gene            complement(1336..2529)
                     /locus_tag="OO596_003219"
     CDS             complement(1336..2529)
                     /locus_tag="OO596_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6719004.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="OO596_003220"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="OO596_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6719005.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="OO596_003221"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="OO596_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6719006.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="OO596_003222"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="OO596_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6719007.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="OO596_003223"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="OO596_003223"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6719008.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="OO596_003224"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="OO596_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6719009.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="OO596_003225"
     CDS             complement(11165..11308)
                     /locus_tag="OO596_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719010.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2927 a         2896 c         2949 g         2770 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tagcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa atgagttgag ag
//
LOCUS       DAJTYZ010000122        11427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000122 DAJTYZ010000000
VERSION     DAJTYZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.122"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="OO596_003226"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="OO596_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6719011.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="OO596_003227"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="OO596_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6719012.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="OO596_003228"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="OO596_003228"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6719013.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="OO596_003229"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="OO596_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6719014.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="OO596_003230"
     CDS             complement(3928..5214)
                     /locus_tag="OO596_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6719015.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="OO596_003231"
     CDS             complement(5235..6026)
                     /locus_tag="OO596_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6719016.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="OO596_003232"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="OO596_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6719017.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="OO596_003233"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="OO596_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6719018.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="OO596_003234"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="OO596_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6719019.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="OO596_003235"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="OO596_003235"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6719020.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="OO596_003236"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="OO596_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6719021.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="OO596_003237"
     CDS             complement(9882..10364)
                     /locus_tag="OO596_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6719022.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11427)
                     /gene="dgcN"
                     /locus_tag="OO596_003238"
     CDS             complement(10380..>11427)
                     /gene="dgcN"
                     /locus_tag="OO596_003238"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCR6719023.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2802 a         2748 c         3061 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgct ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtc
//
LOCUS       DAJTYZ010000123        11357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000123 DAJTYZ010000000
VERSION     DAJTYZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.123"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..537)
                     /gene="espL1"
                     /locus_tag="OO596_003239"
     CDS             complement(<1..537)
                     /gene="espL1"
                     /locus_tag="OO596_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCR6719024.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYV"
     gene            1062..2990
                     /gene="thrS"
                     /locus_tag="OO596_003240"
     CDS             1062..2990
                     /gene="thrS"
                     /locus_tag="OO596_003240"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6719025.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2994..3536
                     /gene="infC"
                     /locus_tag="OO596_003241"
     CDS             2994..3536
                     /gene="infC"
                     /locus_tag="OO596_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6719026.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3633..3830
                     /gene="rpmI"
                     /locus_tag="OO596_003242"
     CDS             3633..3830
                     /gene="rpmI"
                     /locus_tag="OO596_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6719027.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3883..4239
                     /gene="rplT"
                     /locus_tag="OO596_003243"
     CDS             3883..4239
                     /gene="rplT"
                     /locus_tag="OO596_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6719028.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4356..4480
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4362..4406
                     /gene="pheM"
                     /locus_tag="OO596_003244"
     CDS             4362..4406
                     /gene="pheM"
                     /locus_tag="OO596_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6719029.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4689..5672
                     /gene="pheS"
                     /locus_tag="OO596_003245"
     CDS             4689..5672
                     /gene="pheS"
                     /locus_tag="OO596_003245"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6719030.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5687..8074
                     /gene="pheT"
                     /locus_tag="OO596_003246"
     CDS             5687..8074
                     /gene="pheT"
                     /locus_tag="OO596_003246"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6719031.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8079..8378
                     /gene="ihfA"
                     /locus_tag="OO596_003247"
     CDS             8079..8378
                     /gene="ihfA"
                     /locus_tag="OO596_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6719032.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8479..9459
                     /gene="btuC"
                     /locus_tag="OO596_003248"
     CDS             8479..9459
                     /gene="btuC"
                     /locus_tag="OO596_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6719033.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9522..10073
                     /gene="btuE"
                     /locus_tag="OO596_003249"
     CDS             9522..10073
                     /gene="btuE"
                     /locus_tag="OO596_003249"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6719034.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10073..10822
                     /gene="btuD"
                     /locus_tag="OO596_003250"
     CDS             10073..10822
                     /gene="btuD"
                     /locus_tag="OO596_003250"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6719035.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10900..>11357
                     /locus_tag="OO596_003251"
     CDS             10900..>11357
                     /locus_tag="OO596_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6719036.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2873 a         2631 c         3057 g         2796 t
ORIGIN      
        1 gacgtattgc aatgacagat ggcgacaaaa aatagcgtca gaaggagatt gcgaaaaaaa
       61 tgcactacca ttgagattta ctgttggttg tatagttttt tctctatgga tttttttaga
      121 cagtttcata caatggttac gatcttcggc agttaactgt tcctgttcaa tataatccag
      181 tatgcttcgc aaatggttag ccttaatagg cgtggtttcc agaaataagg gaatgatttc
      241 ttttttatag tgtaatgtaa tgagtatatt ttctgcatgg atgggtgaag tggcaaagtt
      301 aatgataaaa agggcagtgg aataacaatt ttgtgaaatt tttcgacagt ttgcggtaaa
      361 ttcctgagta acacagtatg acagcatcga acttaagtca gaggtatcaa gtagggtgat
      421 gagttggcgc gcaaattgat taataggctc tgatgaaggc gttggtatgt cgctatcgat
      481 gtgcaatcta tgaggagact tataatgggt tctgataatg atatcgtttt gactcataat
      541 ctattcgcct tatatatgtc tggaggtttt gaggatgatg attaattatt atcgccagaa
      601 cgaaatgtgc acgaaaaatt gctataagaa ttgtgtcact tttatcattg tgatatgacc
      661 acttcgtcat taatctcatg agcgtttaaa atactaccgc caaatcacgc aacgaacttt
      721 gctggattca gtttatgctg ctgtaaatcc gctcgcagta aacctttcag acgcaccgtg
      781 atgttatcag tggttcttta agcgttttgc tggtgtactc actacaaacg aattgcgaat
      841 cgaatcaatg tgaaacggaa aggtacaatc tccctttttt tagtgttaac atcgttcaac
      901 cgggtggaag gtgagaggtt aatgtctttc atttgtgtac ctgattgcga accaatttag
      961 catttgttgg ctagatggtt tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt
     1021 gatctttcgt gtgggtcacc actgcaaatt aaggatataa aatgcctgtt ataactcttc
     1081 ctgatggcag ccaacgccat tacgaccacg ctgtaagccc catggatgtt gcgctggaca
     1141 ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg
     1201 cttgcgatct gattgaaaac gacgcacaac tgtcgatcat taccgccaaa gacgaagaag
     1261 gtctggagat cattcgtcac tcctgtgcgc acctgttagg gcatgcgatt aaacaacttt
     1321 ggccgcatac caaaatggca atcggcccgg ttattgacaa cggtttttat tacgacgttg
     1381 atcttgaccg cacgttaacc caggaagatg tcgaagcact cgagaagcgg atgcatgagc
     1441 ttgctgagaa aaactacgac gtcattaaga agaaagtcag ctggcacgaa gcgcgtgaaa
     1501 ctttcgccaa ccgtggggag agctacaaag tctccattct tgacgaaaac atcgcccatg
     1561 atgacaagcc aggtctgtac ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg
     1621 taccgaacat gcgtttctgc catcatttca aactaatgaa aacggcaggg gcttactggc
     1681 gtggcgacag caacaacaaa atgttgcaac gtatttacgg tacggcgtgg gcagacaaaa
     1741 aagcacttaa cgcttacctg cagcgcctgg aagaagccgc gaaacgcgac caccgtaaaa
     1801 tcggtaaaca gctcgacctg taccatatgc aggaagaagc gccgggtatg gtattctggc
     1861 acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt tgttcgttct aaactgaaag
     1921 agtaccagta tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa
     1981 ccggtcactg ggacaactac aaagatgcaa tgttcaccac atcttctgag aaccgtgaat
     2041 actgcattaa gccgatgaac tgcccgggtc acgtacaaat tttcaaccag gggctgaagt
     2101 cttatcgcga tctgccgctg cgtatggccg agtttggtag ctgccaccgt aacgagccgt
     2161 caggttcgct gcatggcctg atgcgcgtgc gtggatttac ccaggatgac gcgcatatct
     2221 tctgtactga agaacaaatt cgcgatgaag ttaacggatg tatccgttta gtctatgata
     2281 tgtacagcac ttttggcttc gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac
     2341 gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag
     2401 aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg
     2461 aatttaccct gtatgactgc ctcgatcgtg catggcagtg cggtacagta cagctggact
     2521 tctccttgcc gtctcgtctg agcgcctcct atgtgggcga agacaacgag cgtaaggtac
     2581 cggtaatgat tcaccgcgca attctggggt cgatggaacg tttcatcggt atcctgaccg
     2641 aagaattcgc tggtttcttc ccgacctggc ttgcgccagt tcaggttgtt atcatgaata
     2701 ttaccgattc acagtctgaa tacgttaacg aattgacgca aaaactatca aatgcgggca
     2761 ttcgtgttaa agcagacttg agaaatgaga agattggctt taaaatccgc gagcacactt
     2821 tgcgtcgcgt cccatatatg ttggtctgtg gtgataaaga ggtggaatca ggcaaagttg
     2881 ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga
     2941 agctgcaaca agagattcgc agccgcagtc ttaaacaatt ggaggaataa ggtattaaag
     3001 gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg
     3061 aagttcgctt aacaggtctg gaaggcgagc agcttggtat tgtgagtctg agagaagctc
     3121 tggagaaagc agaagaagcc ggagtagact tagtcgagat cagccctaac gccgagccgc
     3181 cggtttgtcg tataatggat tacggcaaat tcctctatga aaagagcaag tcttctaagg
     3241 aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat taaattccgt cctggtacag
     3301 atgaaggcga ctatcaggta aaactccgca gcctgattcg ctttctcgaa gagggtgata
     3361 aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg
     3421 aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc agtggtcgaa tccttcccaa
     3481 cgaagatcga aggccgccag atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc
     3541 ttcaagtagc actgtctgtg gagccttcgg gcttcacagg ctttgttcgc ctttgtttcg
     3601 tttattaaca atgcgaagtg gaagttatta aaatgccaaa aattaagacc gtacgcggtg
     3661 ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa gcacaagcac gctaacctgc
     3721 gtcacattct gaccaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg
     3781 tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg
     3841 tttttaactt tttaattaga atatagatac aggagagcac atatggctcg cgtaaaacgt
     3901 ggtgttattg cacgcgcacg tcacaagaaa attttgaaac aagctaaagg ctactacggt
     3961 gcgcgttctc gcgtataccg cgttgccttc caggctgtta tcaaagctgg tcagtatgct
     4021 taccgtgacc gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac
     4081 gcagcagcac gtcagaacgg tatttcttac agcaaattca tcaatggcct gaaaaaagcc
     4141 tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag tattcgacaa agtagcgttc
     4201 accgctctgg ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa gagggagcta
     4261 gtctccctct tttcgtttca acgccatcaa aacattgact tttatcgccg tagccttttc
     4321 aataaaggtc ttttgaagag taccaaaagg taacgcaagc aatgaatgct gctattttcc
     4381 gcttcttttt ttactttagc acctgaatcc aggaggctag cgcgtgagaa gagaaacgga
     4441 aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa
     4501 aaagccagtc tatcaggcgt aattgtcgca gccagtctgg acacggccag cgcggagaaa
     4561 ccggagcgta tttaagtacg tgagaatttc gagcactgcc cgggaccaaa atggcaagta
     4621 aaatagcctg atgggatagg ctctaagtcc aacgaaccag tgtcaccact gacacaatga
     4681 ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc gaaggcggcc attagccagg
     4741 cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata tttgggtaaa aaagggcact
     4801 taacccttca gatgacgacc ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg
     4861 cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac
     4921 tggaaagcgc agcgctgaat gcgcgtctgg cggcggaaac gattgatgtc tccctgctag
     4981 gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg taccatcgac cgtatcgaaa
     5041 gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc
     5101 ataacttcga tgctctgaac attcctggtc accacccggc gcgcgctgac cacgacactt
     5161 tctggtttga cactacccgc ctgctgcgta cccagacctc tggcgtacag atccgcacca
     5221 tgaaagccca gcagccaccg attcgtatca tcgcgcctgg ccgcgtttat cgtaacgact
     5281 acgaccagac tcacacgccg atgttccatc agatggaagg tctgattgtt gataccaaca
     5341 tcagctttac caacctgaaa ggcactctgc acgatttcct gcgtaacttc tttgaggaag
     5401 atttgcagat tcgcttccgt ccttcctact tcccgttcac cgagccttct gcagaagttg
     5461 acgtcatggg taaaaacggt aaatggctgg aagtgctggg ttgcgggatg gtgcatccga
     5521 acgtgctgcg taacgttggc atcgacccgg aagtttactc tggtttcgcc ttcgggatgg
     5581 ggatggagcg tctgactatg ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa
     5641 acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa tagattatga aattcagtga
     5701 actgtggtta cgcgaatggg tgaacccggc gattgatagc gatgcgctgg cgaatcaaat
     5761 cactatggcg ggcctggaag ttgacggtgt agaaccggtt gccggtagct tccacggcgt
     5821 ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac gctgacaaac tgcgtgtgac
     5881 aaaagtgaat gtcggcggcg atcgcctgct ggacatcgtc tgtggtgcgc caaactgccg
     5941 tcagggcctg cgtgtggcgg tagctaccat tggtgctgtt ctgccgggtg atttcaaaat
     6001 taaagcggcg aaactgcgtg gcgaaccgtc tgaagggatg ctgtgctcct tctctgaact
     6061 gggaatttct gacgatcaca gcggcattat cgaactgcct gcagatgcgc cgattggcac
     6121 tgacatccgc gaatacctga aactcgatga caacaccatc gaaatcagcg taacgccaaa
     6181 ccgtgccgac tgcttaggta tcattggtgt tgcgcgtgac gttgccgtgc tgaatcagct
     6241 gccgctggtt gaaccggaaa tcgttccggt tggtgcgacc atcgacgaca cgttgccgat
     6301 tacagtcgaa gcgcctgaag cctgcccgcg ctatcttggc cgtgtggtaa aaggcattaa
     6361 cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc
     6421 tatcgatgca gttgttgacg tcaccaacta tgtactgctc gaactgggcc agccgatgca
     6481 cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg cggatggcaa aagagggcga
     6541 aacgctggtg ctgcttgatg gcactgaagc gaagctgaat gctgacactc tggtcatcgc
     6601 cgaccacaac aaggcgctgg cgatgggcgg catctttggt ggcgaacact ctggcgtgaa
     6661 tgacgaaacg caaaacgtct tgctggaatg cgctttcttc agcccgctgt ctatcaccgg
     6721 tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac cgttatgagc gtggcgttga
     6781 tccggcactg cagcacaaag cgatggaacg tgcgacccgt ctgctgatcg acatctgcgg
     6841 tggtgaggct ggtccggtaa ttgatatcac caacgaagca acgctgccga agcgtgcaac
     6901 catcacttta cgtcgtagca aactggatcg cctgatcggc catcatattg ctgatgaaca
     6961 ggtaactgac attctgcgtc gtctcggctg cgaagtgacc gaaggcaaag acgagtggca
     7021 ggcagttgcg ccgagctggc gtttcgatat ggagattgaa gaagatctgg ttgaagaagt
     7081 cgcgcgtgtt tacggctaca acaacatccc ggatgagccg gtacaggcaa gcctgattat
     7141 gggtactcac cgtgaagctg acctgtcgct caagcgcgtg aaaacgctgc tcaacgacaa
     7201 aggctatcag gaagtgatca cctacagctt cgttgatccg aaagtgcagc agatgatcca
     7261 tccaggcgtt gaagccttac tgctgccaag cccgatctct gttgaaatgt cagcaatgcg
     7321 tctttctctg tggaccggcc tgctggcaac cgtggtgtac aaccagaacc gtcagcagaa
     7381 ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca gatactcagg caccgttggg
     7441 cattcgtcag gatctgatgt tagccggtgt gatttgcggt aaccgttacg aagagcactg
     7501 gaacctggca aaagagaccg ttgatttcta tgatttgaaa ggcgatcttg aatccgttct
     7561 cgacctgacc ggtaaactga atgaggttga gttccgtgca gaagcgaatc cggcactgca
     7621 tccggggcaa tccgcagcga tttatctgaa aggggaacgt attggttttg ttggagttgt
     7681 tcatcctgaa ctggaacgta aactggatct taacggtcgc actctggtgt tcgaactgga
     7741 gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc
     7801 gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt cccgcagcgg atattttatc
     7861 cgaatgtaag aaagttggcg taaatcaggt agttggcgta aacttatttg acgtgtaccg
     7921 cggtaagggt gttgcggagg ggtataagag cctcgccata agcctgatcc tgcaagatac
     7981 cagccgtaca ctcgaagaag aggagattgc cgctaccgtc gccaaatgtg tagaggcatt
     8041 aaaagagcga ttccaggcat cattgaggga ttgaacctat ggcgcttaca aaagctgaaa
     8101 tgtcagaata tctgtttgat aagcttgggc ttagcaagcg ggatgccaaa gaactggttg
     8161 aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg
     8221 gttttggtaa cttcgatctg cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg
     8281 gcgaggatat tcccattaca gcacggcgcg tggtgacctt cagacccggg cagaagttaa
     8341 aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc tgatctaact aaaaaggccg
     8401 ctctgcggcc ttttttcttt tcactgtcga agagtcaccg taaaatcaac gccatgacac
     8461 ttcagcagaa cggataccat gctgacactt gcccgccaac aacagcgaca aaatattcgc
     8521 tggttattat gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt
     8581 gaacaatgga tttcgccagg tgactggttt actcctcgtg gcgaactgtt cgtctggcaa
     8641 attcgcctgc cacgtacgct ggctgtattg ctggttggtg cggcgctggc tatatccggc
     8701 gctgtaatgc aggcgttgtt tgaaaatcct ctggcagaac ctggactact tggcgtctct
     8761 aacggcgcag gcgtggggct tatcgccgcg gtattgcttg ggcaagggca actccccaac
     8821 tgggcgctag ggctgtgtgc gattgctggc gcgcttatca tcactttaat actcttacgt
     8881 ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc attagggatt
     8941 atctgtagcg cactaatgac gtgggctatc tacttttcca cctctgttga tttacgtcag
     9001 ctgatgtact ggatgatggg cggttttggc ggcgtagact ggcggcaaag ctggctgatg
     9061 ctggcattga tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat gaatatgtta
     9121 gcacttggcg agatctcggc gcggcaactg ggtttacccc tgtggttctg gcgcaatgtg
     9181 ctggtggcag cgaccggctg gatggttggc gtcagtgtgg cgctggcggg tgctatcggc
     9241 tttattggtc tggtgatccc acatattctt cggttgtgtg gtttaaccga tcatcgcgta
     9301 ttacttcccg gctgcgcgct ggcaggggcg agcgcagtgc tgctggccga tattgtagcg
     9361 cgcctggcat tagctgccgc agagctgcct attggcgtgg tcaccgcaac gttgggtgcg
     9421 ccggtgttta tctggttatt gttaaaagca ggacgttagc cgcaaaaaga cggtctatga
     9481 ttaaaagcta agatttttac tgaccacacc caggagaaac gatgcaagat tccattctga
     9541 cgaccgtagt gaaagatatc gacggtgaag tgaccacgct ggagaagtac gccggtaatg
     9601 tgctgttgat tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat gagcagttgg
     9661 agaatattca gaaagcctgg gccgatcgag gttttgtggt gctgggattc ccgtgcaacc
     9721 agtttctgga acaagaaccg ggcagcgatg aagagattaa aacttactgt accaccacat
     9781 ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa cggcgaagga cgccatccgc
     9841 tgtatcaaaa attgattgcc gcagcgccga ccgcagtcgc gccggaagag agcggattct
     9901 atgcccgtat ggtcagcaaa ggtcgtgcac cgctgtaccc tgatgatatt ttatggaatt
     9961 ttgagaaatt tttagttggc agggacggat tggtcatcca gcgtttttcc ccggatatga
    10021 cgccggaaaa ccccattgtg atggaaagca ttaaactggc gttggcaaaa taatgtctat
    10081 tgtgatgcag ttacaggatg ttgcggaatc tacccgccta gggccacttt ctggcgaggt
    10141 tcgggctggg gagatcctgc acctggtggg gccgaatggc gcgggtaaga gtaccttact
    10201 ggcacgaatg gccggaatga ccagcggtaa gggaagtatt cagttcgcgg ggcaaccact
    10261 ggaagcatgg tccgcaacaa aactcgcgct gcatcgcgcc tatctttcac aacagcagac
    10321 gccgccgttt gcaatgccgg tctggcacta tctgacactg catcagcacg ataaaacgcg
    10381 taccgaacta ctgaatgatg tcgcaggggc gctggctctt gatgacaaac tcggacgtag
    10441 caccaatcaa ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg tggtgctgca
    10501 aatcacacca caagccaatc ccgcaggcca attgctgctt cttgatgagc cgatgaacag
    10561 tcttgatgtt gcgcaacaaa gtgcgttaga caaaattctg agcgctctgt gtcagcaagg
    10621 actggcgatt gtgatgagca gtcacgatct caaccacaca ttgcgtcatg cgcatcgggc
    10681 gtggttgcta aaaggtggaa aaatgctggc cagtggacgc agagaagagg tgctcacgcc
    10741 gccaaatctg gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg aaggtcacag
    10801 aatgctgatt tcgaccatct gatttcatta gtatcttgaa aatcgggtaa ttacgacgct
    10861 aaattaatgc aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg ccttattttg
    10921 atcacagcac tgtttctggc cgggtgtagc caccataaag caccgccgcc aaatgccaga
    10981 ctttctgatt cgattaccgt tattgccggt ttgaacgacc agctacaaag ctggcatggc
    11041 acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg actgttcggg atttgtggtt
    11101 gtaacgatgc gcgatcgttt cgatttgcag ctgccccgag aaaccaaaga acaagcctct
    11161 atcggcacgc aaattgataa agacgagttg ctgcctggtg acctggtctt tttcaaaacg
    11221 ggttccggac aaaatggttt gcatgtaggt atttacgata ccaacaacca atttatccac
    11281 gcctctacca gcaagggagt gatgcgttcc tcacttgata atgtctattg gcagaaaaat
    11341 ttctggcagg cgagacg
//
LOCUS       DAJTYZ010000124        11309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000124 DAJTYZ010000000
VERSION     DAJTYZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.124"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..208
                     /locus_tag="OO596_003252"
     CDS             <1..208
                     /locus_tag="OO596_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6719037.1"
                     /translation="IVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFA
                     QTTKGVLETGIYRGVVSIYLSQDI"
     gene            300..2984
                     /locus_tag="OO596_003253"
     CDS             300..2984
                     /locus_tag="OO596_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6719038.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2981..4069
                     /locus_tag="OO596_003254"
     CDS             2981..4069
                     /locus_tag="OO596_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6719039.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="OO596_003255"
                     /pseudo
     CDS             complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="OO596_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5932..7344
                     /gene="uxaC"
                     /locus_tag="OO596_003256"
     CDS             5932..7344
                     /gene="uxaC"
                     /locus_tag="OO596_003256"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6719040.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7359..8846
                     /gene="uxaA"
                     /locus_tag="OO596_003257"
     CDS             7359..8846
                     /gene="uxaA"
                     /locus_tag="OO596_003257"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6719041.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8929..9480
                     /locus_tag="OO596_003258"
     CDS             8929..9480
                     /locus_tag="OO596_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6719042.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9485..10729)
                     /gene="sstT"
                     /locus_tag="OO596_003259"
     CDS             complement(9485..10729)
                     /gene="sstT"
                     /locus_tag="OO596_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6719043.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2969 a         2773 c         2934 g         2633 t
ORIGIN      
        1 gattgttccg ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac
       61 ttttaccgca accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa
      121 cctgatgttt gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt
      181 aagcatttat ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg
      241 aggggtagcc ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca
      301 tggataaaaa attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat
      361 taaccgaaat tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg
      421 tttatttttc agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa
      481 catttcttga tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa
      541 tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcaccacggt gaattggctt
      601 gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa
      661 tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt
      721 tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc
      781 agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg
      841 gtgcgctggg aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg
      901 atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt
      961 ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac
     1021 aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg
     1081 ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc
     1141 tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt
     1201 atctcaatag tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg
     1261 tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca
     1321 aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat
     1381 cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc
     1441 cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag
     1501 gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt
     1561 tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat
     1621 ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca
     1681 gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt
     1741 ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt
     1801 tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa
     1861 gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt
     1921 tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg
     1981 gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc
     2041 attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat
     2101 ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg
     2161 tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat
     2221 acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt
     2281 cggcctttac gggcacttat agctctacgc tggctgtcag ttgctatggc gttaatttgg
     2341 gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg
     2401 aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt
     2461 tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg
     2521 ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg
     2581 cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca
     2641 gctttaacta caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga
     2701 ttgtggggct gaacagcgcc atgttactgg tagctgaaga tggaggattt acacttgaaa
     2761 ttaacggcag cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac
     2821 tgagtgttgt aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg
     2881 tgacttattc acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat
     2941 tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc
     3001 ggcgatattg ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag
     3061 tgaaattacg tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc
     3121 gtcggcagga aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac
     3181 ctgtacagct aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg
     3241 gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc
     3301 gccgccagga tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc
     3361 ttcatgtaca gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta
     3421 ccttgagcag acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa
     3481 attacattcg aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga
     3541 ccccaaccat attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt
     3601 agacttgcat ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt
     3661 ggacatgtgc ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa
     3721 agatgaaggg cgaccagctg cagggcgcag agacggtgac ttctctatct atcgtcaagg
     3781 agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga
     3841 aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct
     3901 gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac
     3961 acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat
     4021 gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattgac cccgcagacc
     4081 agaaaaaact aaacaacgaa gccgcctgat aaggcggctt cgtcattaca acttacgtta
     4141 aacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
     4201 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
     4261 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
     4321 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     4381 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
     4441 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
     4501 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
     4561 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     4621 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     4681 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgccagg
     4741 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     4801 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     4861 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     4921 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
     4981 gccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtagc
     5041 ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg
     5101 ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagccagg ccaccccagc
     5161 tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac attgcataac
     5221 cgattttcgt acccagcaca tccagtacat aacctgctac cggttgcatg accgtataag
     5281 cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca
     5341 tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccga
     5401 gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca
     5461 tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag
     5521 ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt
     5581 gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat
     5641 ttgcattccg tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa
     5701 aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag
     5761 gccagtactg gcgcggcctg cagcgagatt tatcactttg agagtaattt ttttaactac
     5821 gtttattgat ctaactcacg aaaatatctt cggactctgg aaattggtgt gataactttg
     5881 tcagcatcgc accataagca agctagctca ctcgttgaga ggaagacgaa aatgactccg
     5941 tttatgactg aagatttcct gttagatacc gaatttgccc gccgtctgta tcacgactac
     6001 gcaaaagacc agccgatttt cgattaccat tgccatttgc cgccgcagca gattgcggaa
     6061 gactatcgtt ttaaaaacct gtatgacatc tggctgaaag gcgatcacta caaatggcgc
     6121 gctatgcgta ccaacggtgt ggccgagcgt ctgtgtaccg gtgatgcatc tgaccgtgaa
     6181 aaatttgacg cctgggcggc gactgttccg cacactatcg gcaacccgtt ataccactgg
     6241 acgcaccttg aactgcgtcg tccgtttggt atcactggca aattgctttc tccgtcaact
     6301 gccgatgaaa tctggaacga atgtaacgaa ctgctggcgc aggataactt ctccgcgcgc
     6361 ggcatcatgc agcagatgaa cgtgaaaatg gtcggcacca ccgatgaccc gatcgattct
     6421 ctggagcatc acgcagagat cgccaaagac ggctctttca ccatcaaagt gctgccgagc
     6481 tggcgtccgg acaaagcctt caacatcgaa caggcgacct ttaacgacta catggcgaag
     6541 ctgggcgaag tttccgatac cgacattcgc cgctttgctg acctgcaaac tgccttgacc
     6601 aaacgtctgg atcacttcgc cgctcacggc tgtaaagtgt ctgaccatgc gctggacgtg
     6661 gtgatgtttg ctgaagcgaa cgaagcggaa ctggacagca ttctggcgcg ccgtctggct
     6721 ggcgaaaccc tgagcgagca cgaagtggca cagttcaaaa ctgcggtact ggtgttcctt
     6781 ggtgccgaat atgcacgtcg cggctgggta cagcagtacc acattggcgc gctgcgtaat
     6841 aacaacctgc gtcagttcaa actgctgggg ccggatgtag gctttgactc catcaacgac
     6901 cgtccgatgg cggaagagtt gtctaagctg ctgagcaagc agaacgaaga aaacctgctg
     6961 ccgaaaacca ttctctactg cctgaacccg cgcgataacg aagtgctggg caccatgatc
     7021 ggtaacttcc agggcgaagg tatgccgggc aaaatgcagt tcggttccgg ctggtggttt
     7081 aacgatcaga aagacggtat ggaacgtcag atgacccaac tggcgcagct cggtctgctg
     7141 agccgctttg tcggtatgct gactgacagc cgtagcttcc tgtcatacac ccgtcacgaa
     7201 tacttccgcc gcattctgtg ccagatgatc ggtcgctggg tggaagcggg cgaagcaccg
     7261 gcggacatca acctgctggg cgagatggtg aaaaatattt gctttaacaa tgcgcgtgac
     7321 tacttcgcca ttgaactgaa ctaaggtctg gggttgatat gcaatacatc aagatccatg
     7381 cgctggataa cgtcgcggtc gctttagcag atttggctga aggcacagaa gtcagtgtcg
     7441 ataaccagac tgttacgctg cgccaggatg ttgctcgtgg acataaattt gcgttaacgg
     7501 atatcgcaaa aggggccaac gtcatcaaat atggcctgcc gattggttat gcattggcgg
     7561 atattgcggc gggggaacac gtccacgccc acaatacgcg cacgaatctg agcgatctgg
     7621 atcagtatcg ctatcaacct gattttcagg atctgcctgc gcaagcggca gatcgtgaag
     7681 tgcagatcta tcgtcgcgct aacggcgatg tcggggtgcg taacgagctg tggatcctgc
     7741 caaccgtggg ctgtgtcaac ggcatcgcgc ggcaaatcca gaaccgtttc ctgaaagaga
     7801 ccaacaacgc cgaaggtacc gacggcgtgt tcctcttcag ccacacctac ggctgctcac
     7861 aactgggcga cgatcacatc aacatccgca ccatgctgca aaacatggtg cgccacccga
     7921 acgcgggcgc agtgctggtg attggtctgg gctgtgaaaa caaccaggtt gccgcattcc
     7981 gtgaaacgtt gggcgatatc gatcctgaac gcgttcattt catgatctgc caacagcagg
     8041 atgatgagat cgaagccggg atcgagcatt tgcatcagct gtataacgtg atgcgcaacg
     8101 ataaacgcga gccaggcaaa ctcagcgaac tgaagtttgg tctggagtgc ggtggttctg
     8161 acggtctttc tggtattact gctaacccga tgctggggcg tttctctgac tacgtgattg
     8221 ctaacggcgg tactaccgta ctgaccgaag tgccggagat gtttggcgca gagcagttgc
     8281 tgatggacca ttgccgcgac gaagcaacgt ttgaaaaact ggtcaccatg gtcaacgact
     8341 ttaaacagta ctttattgcc catgatcagc cgatctacga aaacccatca ccggggaaca
     8401 aagcgggcgg tatcaccacg ctggaagaca aatcacttgg ctgtacccag aaagcgggtt
     8461 ccagtgtcgt ggttgacgtg ctgcgttacg gcgagcgtct gaaaacgcct gggctgaact
     8521 tgttaagtgc gccgggtaac gatgccgtag cgaccagcgc cctggcgggt gcgggctgcc
     8581 atatggtgct gttcagtact ggtcgcggca cgccatacgg tggatttgtg ccgacggtga
     8641 aaatcgccac caacagtgaa ctggcggcga agaaaaaaca ctggatcgac tttgacgcgg
     8701 gtcagctgat ccacggtaaa gcgatgccgc agttgctgga agaatttatc gacaccatcg
     8761 ttgagtttgc caacggtaag caaacttgca acgagcgtaa cgacttccgc gaactggcga
     8821 tcttcaaaag cggcgtaacg ctataaagtc gaaatgagtt gttaataaaa aacggcgttt
     8881 cataatgtga agcgccgttt gcttttccat acctgataag gatttatcat gaccgcgtat
     8941 tggctggccc agggcgtggg tgtcatcgcc tttctgattg gtatcacaac atttttcaat
     9001 cgtgacgaac gtcgcttcaa aaagcagctt tcggtctata gcgccgttat tggcgtacat
     9061 ttttttcttc tgggcaccta tcccgctggt gccagtgcca tccttaatgc cattcgtaca
     9121 ttgattacct tacgcacgcg cagcttatgg gtaatggcga tttttattgt gctgactggc
     9181 ggaattggcc tcgcgaagtt ccatcatcct gtcgaactat tgccggttat cggtacgatt
     9241 gtcagtacct gggcgctgtt ccgctgtaaa gggctcacca tgcgctgcgt gatgtggttt
     9301 tcaacgtgtt gctgggtgat tcacaacttc tgggcggggt cgataggcgg cacaatgatt
     9361 gagggtagtt ttctgcttat gaatggcttg aatatcattc gtttctggcg gatgcagaaa
     9421 aggggaattg atccgtttaa agtagagaaa acccctcccg ccgtagacga aaggggttaa
     9481 acaattaatt acgcagggcg ctatttgcca gacgatcgtc ttccgcctgg caagctgccg
     9541 cagtgaacag cacgtcagtt gaagagttca gcgcggtttc gcaagagtcc tgcaatacgc
     9601 cgatgataaa gccgacggca accacctgca tggcgatatc gttcgaaata ccgaacatat
     9661 tacaggccag cgggatcagc agcagagaac cccccgccac gccggatgcg ccacaggcac
     9721 acagagaagc caccacgctc aacagcagcg ccgtgggcag atcgaccggg atacccagcg
     9781 tattaaccgc agccagcgtc aacacggtaa tggtgattgc tgcgcccgcc atattgatgg
     9841 tggcacccag cggaatagaa acggaatagg tatcgcgatc cagattcagc ttttcacaca
     9901 gtgccatatt caccggaatg ttcgccgcag agctgcgggt gaagaaggca tacacgccgc
     9961 tttcgcgcag gcacagcagc accagcggga acgggttacg acgaattttc caccacacca
    10021 gcaatgggtt aaccaccagc gccaccagta acatacagcc aaccagcacg accagcagtt
    10081 gcgcgtagcc ccacagtgtg gagaaaccgg tggttgccag ggtagaagaa accagaccaa
    10141 aaataccaat cggtgcgaag cgaatgacca gtttcaccat aaaggtgacg gcattcgaca
    10201 tatcgttgac caggtttttg gtggtctcgt taccgtgacg cagtgcgaaa ccgaggccaa
    10261 ttgcccacac caggatcccg atgtagttac ctttcagcag cgcgtcgatg gggttggaaa
    10321 ccatgctcat taccagcccg cgcatcactt ctacaatgcc tgacggtggc gaaatatcac
    10381 ccgcgctact ggataagtgc agggtagaag ggaaggcgaa gctgaaaacg actgcggcca
    10441 gagcagcaga gaaggtaccc agcagataga ggaacaaaat agggcggata ttggttttct
    10501 gcccatgctg gtggttagca atagatgcca tcaccagcat caacaccagg atgggggcaa
    10561 cggctttcag tgcgccgacg aacaaagtac ctaacagacc aacagcttcc gccgcgggtt
    10621 ttgatatcca tgccagaaga atccccagaa caaggccgac caggatttgt tttaccaggc
    10681 tgccatgagc cagacgccgg aatagccccg gtgaacgttg cgtagtcatt tttcgatcct
    10741 ttcattgtgt tatcgcacat ccctggtgcg ttctgtcgac gcatctttca ggatgtaaca
    10801 aagtgtttgc aaggtcgagt ataaggaaag ttacagcttc gggaagcgaa aaatgctgga
    10861 ttttaaagag aagtcatatt ttgaaactta atggttacat ttgtttgaca taaacagagg
    10921 gaaggactgg ctactaaggc tgattagata acatatgggc aaaactattt ggaatatgag
    10981 aacggttgcg ctcctttaat gtgctatgcc atataaagtc actggcgcag ggagcgcgca
    11041 gggggcggcc aatcgccgcc gccccctgca cccccaggct ctggcgaaca aaatcgccgc
    11101 tgcgcgattc cctcagctta tcccttgcgg ctaccgggtc gggcgcaagg gaacgtccct
    11161 gtaagatgcg ccctcgccgc gcatccatgc gcgtcgcccc ggcctccagg gaacgcttcg
    11221 gcgattttga cgccaccaac acccgtgcgg tctttttgta aagagctaca agccgattac
    11281 ataattaata gctcaccttt gtctggaat
//
LOCUS       DAJTYZ010000125        11290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000125 DAJTYZ010000000
VERSION     DAJTYZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.125"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            486..1157
                     /locus_tag="OO596_003260"
     CDS             486..1157
                     /locus_tag="OO596_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCR6719044.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1167..2363
                     /locus_tag="OO596_003261"
                     /pseudo
     CDS             1167..2363
                     /locus_tag="OO596_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2363..2923
                     /locus_tag="OO596_003262"
     CDS             2363..2923
                     /locus_tag="OO596_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6719045.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2925..3698
                     /locus_tag="OO596_003263"
     CDS             2925..3698
                     /locus_tag="OO596_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCR6719046.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3886..4161
                     /gene="frmR"
                     /locus_tag="OO596_003264"
     CDS             3886..4161
                     /gene="frmR"
                     /locus_tag="OO596_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCR6719047.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4196..5305
                     /gene="frmA"
                     /locus_tag="OO596_003265"
     CDS             4196..5305
                     /gene="frmA"
                     /locus_tag="OO596_003265"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCR6719048.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5398..6231
                     /gene="fghA"
                     /locus_tag="OO596_003266"
     CDS             5398..6231
                     /gene="fghA"
                     /locus_tag="OO596_003266"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6719049.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6457..6996)
                     /locus_tag="OO596_003267"
     CDS             complement(6457..6996)
                     /locus_tag="OO596_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCR6719050.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OO596_003268"
     CDS             complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OO596_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCR6719051.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OO596_003269"
     CDS             complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OO596_003269"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCR6719052.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="OO596_003270"
     CDS             complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="OO596_003270"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCR6719053.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10553..>11290)
                     /gene="mhpD"
                     /locus_tag="OO596_003271"
     CDS             complement(10553..>11290)
                     /gene="mhpD"
                     /locus_tag="OO596_003271"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCR6719054.1"
                     /translation="PLRDLIGIDHAEAAYAIQHINVQYDVVQGRRVVGRKVGLTHPKV
                     QQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQPRIEAEIALVLNRDLPATDITFDEL
                     YNAIEWVLPALEVVGSRIRDWSIQFVDTVADNASCGVYVIGGPAQRPAGLDLKNCAMK
                     MTRNNEEVSSGRGSECLGHPLNAAVWLARKMASLGEPLRAGDIILTGALGPMVAVNAG
                     DRFEAHIEGIGSVAATFSSAAPKGSLS"
BASE COUNT         2776 a         3005 c         2754 g         2755 t
ORIGIN      
        1 aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt ttagtgatgc
       61 ataaacaacc acattacgag atttattact tcattgtttc tatcaataca tataataaaa
      121 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
      181 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
      241 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat accaaacata
      301 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
      361 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
      421 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      481 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      541 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      601 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      661 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      721 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      781 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      841 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      901 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      961 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     1021 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     1081 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     1141 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
     1201 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     1261 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     1321 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1381 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1441 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1501 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1561 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1621 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1681 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1741 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1801 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1861 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1921 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1981 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     2041 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     2101 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     2161 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     2221 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     2281 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     2341 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2401 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2461 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2521 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2581 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2641 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2701 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2761 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2821 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2881 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2941 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     3001 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     3061 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     3121 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     3181 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     3241 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     3301 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3361 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3421 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3481 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3541 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3601 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3661 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3721 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3781 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3841 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3901 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3961 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     4021 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     4081 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     4141 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     4201 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     4261 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     4321 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4381 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4441 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4501 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4561 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4621 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4681 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4741 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4801 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4861 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4921 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4981 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     5041 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     5101 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     5161 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     5221 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     5281 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     5341 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5401 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5461 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5521 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5581 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5641 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5701 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5761 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5821 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5881 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag
     5941 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     6001 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     6061 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     6121 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     6181 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     6241 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6301 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6361 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6421 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6481 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6541 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6601 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6661 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6721 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6781 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6841 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6901 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6961 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7021 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     7081 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7141 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     7201 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     7261 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     7321 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7381 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7441 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7501 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7561 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7621 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7681 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7741 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7801 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7861 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7921 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7981 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     8041 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     8101 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     8161 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     8221 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     8281 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     8341 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8401 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8461 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8521 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8581 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8641 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8701 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8761 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8821 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8881 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8941 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     9001 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     9061 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     9121 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     9181 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     9241 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     9301 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9361 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg
     9421 tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat
     9481 cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata
     9541 ctgatgacga atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt
     9601 accgttcatg ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc
     9661 gccagcgcac tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct
     9721 gcgccttcga cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt
     9781 aaattgaccg gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa
     9841 cccggtacgt aagcctgcac cgcctcagcc atttcattga ttgaggcttc gatatcatct
     9901 tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca tcatcaacgg tggctctgct
     9961 gggttaagca caataatcgc cttccctttt gccgcgccgc ccaccacttc aatggctcgg
    10021 gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc caggtccggc agatttactg
    10081 gcgatagaag cgataatttc ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc
    10141 attggaatgg tggcctggcc gccgcaggtg accatgttga cgttcagttg atcgacgttc
    10201 gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt
    10261 aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg
    10321 ctggtcgcat caaatacaat gtcgatatca gcaaattcag gcatgttcat cagcccgatc
    10381 accccttcat gggtggtggc gacgcccata cgtctggcac gcgccagacc gtcggactga
    10441 ggatcaatgc cgaccatcac cgccatctcc agatgctgac cgtgacgcaa aattttaatc
    10501 atcagatcgg tgccaatgtt gccagaaccg ataatggcga ctttacgctt actcatgaca
    10561 gacttccttt tggggctgcg cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat
    10621 gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg gcctaatgcc ccggtaagaa
    10681 tgatatctcc ggcgcgcagc ggttcaccca gactggccat tttgcgtgcc agccagacgg
    10741 cggcattaag cggatgtccc aggcattcgc tgccgcgccc gctggaaacc tcttcgttat
    10801 tacgcgtcat cttcatggcg cagtttttca ggtctaaccc cgccggacgt tgcgccggac
    10861 cgccgatgac atacaccccg caggaagcgt tatctgccac ggtatcgaca aactgaatcg
    10921 accagtcgcg aatgcggctc cccaccactt ccagcgccgg aagtacccat tcaatggcgt
    10981 tatacagttc gtcgaaggtg atatcagttg cgggtaaatc gcggttcaac accagtgcga
    11041 tctccgcttc aatgcggggt tgcagaacac gggaaaaagg aatgatttcg ttatcgccat
    11101 aacacatgtc ggcaaataac gtcccaaaat ccggttgatc aacgcccagt tgttgttgca
    11161 ctttcggatg cgtcaggccc actttacgcc ctaccacgcg acgcccctgc acaacgtcat
    11221 attgcacatt tatgtgctga atggcgtagg ccgcttcagc gtgatcgata ccaatcagat
    11281 cgcgcagcgg
//
LOCUS       DAJTYZ010000126        11232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000126 DAJTYZ010000000
VERSION     DAJTYZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.126"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..298
                     /locus_tag="OO596_003272"
     CDS             <1..298
                     /locus_tag="OO596_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6719055.1"
                     /translation="GKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFFDQQ
                     IRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            497..778
                     /gene="ppiC"
                     /locus_tag="OO596_003273"
     CDS             497..778
                     /gene="ppiC"
                     /locus_tag="OO596_003273"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6719056.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(865..2340)
                     /gene="ilvC"
                     /locus_tag="OO596_003274"
     CDS             complement(865..2340)
                     /gene="ilvC"
                     /locus_tag="OO596_003274"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6719057.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2490..3383
                     /gene="ilvY"
                     /locus_tag="OO596_003275"
     CDS             2490..3383
                     /gene="ilvY"
                     /locus_tag="OO596_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6719058.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3435..4979)
                     /gene="ilvA"
                     /locus_tag="OO596_003276"
     CDS             complement(3435..4979)
                     /gene="ilvA"
                     /locus_tag="OO596_003276"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6719059.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4982..6832)
                     /gene="ilvD"
                     /locus_tag="OO596_003277"
     CDS             complement(4982..6832)
                     /gene="ilvD"
                     /locus_tag="OO596_003277"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6719060.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6897..7826)
                     /gene="ilvE"
                     /locus_tag="OO596_003278"
     CDS             complement(6897..7826)
                     /gene="ilvE"
                     /locus_tag="OO596_003278"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6719061.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7846..8109)
                     /gene="ilvM"
                     /locus_tag="OO596_003279"
     CDS             complement(7846..8109)
                     /gene="ilvM"
                     /locus_tag="OO596_003279"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6719062.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8106..9752)
                     /locus_tag="OO596_003280"
     CDS             complement(8106..9752)
                     /locus_tag="OO596_003280"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6719063.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9755..9805)
                     /locus_tag="OO596_003281"
     CDS             complement(9755..9805)
                     /locus_tag="OO596_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719064.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9892..9990)
                     /gene="ilvL"
                     /locus_tag="OO596_003282"
     CDS             complement(9892..9990)
                     /gene="ilvL"
                     /locus_tag="OO596_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6719065.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10343..11232
                     /locus_tag="OO596_003283"
                     /pseudo
     CDS             10343..11232
                     /locus_tag="OO596_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2553 a         3087 c         2947 g         2645 t
ORIGIN      
        1 cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc gcgatcatga
       61 caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg acctgcaatt
      121 ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca gtcgaaaccg
      181 attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac atgagttggt
      241 tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca taaattgaca
      301 ataaaataac catccccgat aatgactttg tagcccatag aggccctaag ttttctcccc
      361 ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac cagcccctcc
      421 ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct gacaattatt
      481 cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg taaaagaaga
      541 gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg gcaagctggc
      601 gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg aattccgcca
      661 gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac tggagccaac
      721 cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc gtaactaata
      781 gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt gggagagggt
      841 cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca tatccgtcat
      901 atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg cttcgttcac
      961 atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca gatcgcccgg
     1021 ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga acagatagtt
     1081 accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct tacgggcgat
     1141 ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt cttcgatgat
     1201 gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca tcgcgatcat
     1261 cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact gcggcgcggt
     1321 ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat catcgttggc
     1381 ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca tatgtttctg
     1441 gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac gcagtttcgc
     1501 cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg cttcggtgat
     1561 ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc cctcttccac
     1621 cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga tggtttgctc
     1681 acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc ccgcacggtg
     1741 accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt tcggatcgtt
     1801 ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact cttcacgtac
     1861 ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac ggatctgctc
     1921 gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt ctttcatcag
     1981 tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt taaccaccag
     2041 atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt cggtcgcttt
     2101 acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg agatatcgag
     2161 accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc cgacgataac
     2221 cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc ccataaagcg
     2281 acatttgccc agctgtgcca gctgctggcg cagattcagt gtattgaagt agttagccat
     2341 ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga gattgaattc
     2401 actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac attgcaattt
     2461 ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa accttcctgc
     2521 atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt agcccatcca
     2581 cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta tttgtgcgcg
     2641 ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc gcccagcaaa
     2701 cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg tcgctctctg
     2761 gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg ccgattctgg
     2821 atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt gatgcggcag
     2881 atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg aaaccggaga
     2941 cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg ctgattgccc
     3001 ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac tggtcaacgg
     3061 tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg tggtttcgac
     3121 gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa gcgatggtat
     3181 cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg gaaaacagcc
     3241 cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa acgccgtttg
     3301 aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag gcgttctgga
     3361 ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag cgaagcgcta
     3421 tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt ggtttcgtcg
     3481 tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg gtcgccgagt
     3541 tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg gaacaaagaa
     3601 atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg tgattccggg
     3661 aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc gaccatatag
     3721 cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta gccgccgtcg
     3781 ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg cacgccgaca
     3841 aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac cgatcgcccg
     3901 ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt caccgccaac
     3961 aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag gccgtggaag
     4021 ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt gtgctgagcg
     4081 atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac tgcgcgcaca
     4141 tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt gatgatgtcg
     4201 tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac cgcgacacct
     4261 tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag cgctgctttc
     4321 aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat cagttgtttg
     4381 atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa tacgcggtcg
     4441 agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc aatcaccatc
     4501 ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc gatcgcttta
     4561 gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa tccacgtacc
     4621 gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt cacgcctaac
     4681 cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt gatcacgccg
     4741 tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg cagcttaaag
     4801 ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag acgcgacgac
     4861 agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg cgcgcgcagc
     4921 actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga gtcagccatt
     4981 attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg gttgccaggc
     5041 tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc gtccaggctt
     5101 tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg cttacctgta
     5161 actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca atcagaccaa
     5221 tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca gaggtgccgc
     5281 cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt gatttcagga
     5341 agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct tcatagcgga
     5401 tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc gcgtcgtcct
     5461 ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg acgcccgctg
     5521 ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg ccgccgtctt
     5581 tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca tcgagagaat
     5641 cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg cctgcgcgga
     5701 acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc agagtttgcg
     5761 gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg cgatccagtt
     5821 caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg tatttttggg
     5881 tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg atatcactca
     5941 tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc gtgttggtag
     6001 agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg ctagcaatat
     6061 tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat tcaacaatgc
     6121 gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc agcgagccgt
     6181 tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta gcggtaaaca
     6241 tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg ctctgggagt
     6301 cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc ttgatgatct
     6361 gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag atcaccggaa
     6421 tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag ttagagatgc
     6481 agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca gcgatcagtt
     6541 cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca tcatccaccg
     6601 caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg gcgaccagtt
     6661 taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag ttcacaaccg
     6721 cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac agcgcacgag
     6781 cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc atactttatt
     6841 tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt ttgtatttat
     6901 tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa gaggccgaag
     6961 aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac ctgaataccg
     7021 tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac ttcatccgcc
     7081 aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag ctctttcgcc
     7141 agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa cggtggggtg
     7201 aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga gatgtaaccg
     7261 ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct acccaccagc
     7321 agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt tggtgctgcg
     7381 cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc gcccagatac
     7441 gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg cgggtttact
     7501 cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt gagattattt
     7561 ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga aaccgggaag
     7621 cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg gaatacaacc
     7681 ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc atagtgcagc
     7741 gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc attgaaccaa
     7801 atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg cgcggatttg
     7861 ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca gtttatttaa
     7921 ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga tatttatatt
     7981 ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac gatgacgcac
     8041 cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat tgacctgatg
     8101 ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg caccagcggc
     8161 cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc actgttcagc
     8221 atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc agggatgccg
     8281 aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc gctgtatcgt
     8341 tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt atcgagtaag
     8401 acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac attcatcatg
     8461 aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac ttgtgcgcca
     8521 accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat gaaattttcc
     8581 gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac atctgtggtc
     8641 acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg cgcgtagata
     8701 gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc gcagtattgc
     8761 tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt taaatcaccc
     8821 tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat atccatatgg
     8881 ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc atcaaaacgt
     8941 gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc tttgatgccg
     9001 tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc cagccctttc
     9061 agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc cggaactgcc
     9121 tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt tgccagcatc
     9181 tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac ggtggtgaac
     9241 cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac cagaaccgga
     9301 ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg caactcttcc
     9361 agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag gacatccact
     9421 tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat ggcaacaaca
     9481 gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc gcccggacca
     9541 gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat agccgccatt
     9601 gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata caatgcatcg
     9661 taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc ctgtgcccgc
     9721 aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg aatcttgaga
     9781 aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta aggtcaaaaa
     9841 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
     9901 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
     9961 taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    10021 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    10081 atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa tagtgagtga
    10141 aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc tttgtccgct
    10201 gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc acaaataaac
    10261 aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc ttatgcacat
    10321 aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg cagccctggg
    10381 agtaaatgcg ccccctgatc actgttgagg tacatatcag taaaggtcta cccggcttaa
    10441 cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg cgcagcgcca
    10501 ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt gctccagccg
    10561 atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg ctggcggcct
    10621 cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa ctggctctta
    10681 caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct attaagtcgg
    10741 gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt aacggtgaag
    10801 gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt aagcacgctc
    10861 tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat ctcagtgatg
    10921 ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc aggcacaacc
    10981 ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt attaatggcc
    11041 tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta agtctggtaa
    11101 atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct catcacagtg
    11161 cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa atttcgttgg
    11221 cgcataacgg cg
//
LOCUS       DAJTYZ010000127        11225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000127 DAJTYZ010000000
VERSION     DAJTYZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.127"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..390
                     /locus_tag="OO596_003284"
     CDS             <1..390
                     /locus_tag="OO596_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6719066.1"
                     /translation="ALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEK
                     QKPYYSVSSLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETA
                     QMVYEIYGMWIDDMNDEQIAMLNARLS"
     gene            442..1377
                     /gene="ttcA"
                     /locus_tag="OO596_003285"
     CDS             442..1377
                     /gene="ttcA"
                     /locus_tag="OO596_003285"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6719067.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1506..2879)
                     /gene="dbpA"
                     /locus_tag="OO596_003286"
     CDS             complement(1506..2879)
                     /gene="dbpA"
                     /locus_tag="OO596_003286"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6719068.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3357..4340)
                     /gene="zntB"
                     /locus_tag="OO596_003287"
     CDS             complement(3357..4340)
                     /gene="zntB"
                     /locus_tag="OO596_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6719069.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4595..5827
                     /gene="dgcM"
                     /locus_tag="OO596_003288"
     CDS             4595..5827
                     /gene="dgcM"
                     /locus_tag="OO596_003288"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR6719070.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5848..6411)
                     /gene="smrA"
                     /locus_tag="OO596_003289"
     CDS             complement(5848..6411)
                     /gene="smrA"
                     /locus_tag="OO596_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6719071.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6741..7649)
                     /locus_tag="OO596_003290"
     CDS             complement(6741..7649)
                     /locus_tag="OO596_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6719072.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7825..9135
                     /gene="abgA"
                     /locus_tag="OO596_003291"
     CDS             7825..9135
                     /gene="abgA"
                     /locus_tag="OO596_003291"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCR6719073.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPTSALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9135..10580
                     /gene="abgB"
                     /locus_tag="OO596_003292"
     CDS             9135..10580
                     /gene="abgB"
                     /locus_tag="OO596_003292"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR6719074.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10617..>11225
                     /locus_tag="OO596_003293"
     CDS             10617..>11225
                     /locus_tag="OO596_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCR6719075.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFN"
BASE COUNT         2860 a         2865 c         2784 g         2716 t
ORIGIN      
        1 gcactgaagg agcaatacaa actgaccggg catcatcgca aaagcgaaat cactttttat
       61 catcgggagt acggcagaac tgaaaagcaa aaactgcatt ttgttttcat gcccagggtg
      121 tgtaacgaaa aacagaaacc ttattactcg gtaagcagtt tgggtgcaag atggaatgca
      181 gcagtaaaac gtgctggtat tcgccgccgt aatccgtacc atacgcgaca tacttttgcc
      241 tgctggctgt tgacggcagg agcgaacccg gcatttatag ccagccagat ggggcatgaa
      301 actgcgcaaa tggtgtatga aatttacggt atgtggattg atgacatgaa cgacgaacag
      361 atagccatgt tgaatgcgcg gttatcgtag ttgcaaagtt tgcccccaat ttgccccatt
      421 tagtaccaga gaactgaaat aatgcaagaa aatcaacaaa ttacaaagaa agaacaatac
      481 aacctgaaca aattacaaaa acgtctgcgt cgtaacgtgg gcgaagccat tgctgacttc
      541 aatatgattg aagaaggcga tcgcatcatg gtttgcctct ccgggggtaa agacagctat
      601 accatgctgg agattctgcg caatttgcag caaagcgcgc caatcaattt ttcgctggtg
      661 gctgttaacc tcgatcaaaa gcaaccgggc ttcccggaac acgttctgcc cgagtatctt
      721 gaaaagctgg gcgttgagta caagattgtc gaagagaaca cttacggtat cgtgaaagag
      781 aagattccag agggcaaaac cacttgctca ctgtgttctc gccttcgtcg cggtatcctt
      841 tatcgtaccg caacggaact gggggcgacg aagatcgcgc tgggtcacca tcgtgacgat
      901 atcctgcaga cgctgttctt aaatatgttc tacggcggca agatgaaagg tatgcctccg
      961 aaactgatga gcgatgatgg caaacatatc gttattcgtc cgctggccta ctgccgcgag
     1021 aaagatattc agcgatttgc cgatgcaaaa gcgttcccga ttattccgtg caacctgtgc
     1081 ggttcacagc ctaacctgca acgtcaggtg attgctgaca tgttgcgtga ctgggataaa
     1141 cgttatccag ggcgtatcga gacgatgttc agcgcgatgc agaatgtggt gccgtcgcat
     1201 ctgtgcgata ccaacctgtt cgatttcaaa ggcatcaccc acggttctga agtggttaac
     1261 gggggtgatc tggcgtttga tcgcgaagag atcccactac aaccggcggg ctggcagcca
     1321 gaagaagatg aaaatcagtt ggatgagtta cggctgaatg tggttgaagt gaaataacca
     1381 ggatggcgcc cgatgcgcaa gcgtatcggg ctactcttat ggaggccgga taagacgcgg
     1441 ccagcgtcgc atccggcaat cccgaataag atgtttactc ttgcacccgg caattcaaca
     1501 tttcattatt ttaataaccg cacccggctc gtttttcctt taatcttccc gccctgtaac
     1561 tgtttccatg ctttatgagc aacagcctga cggaccgcga catagacatg cgccggatgc
     1621 acggcgattt tgccaatatc tgcgccatca agcccgatat ctcctgtcag tgcacctaat
     1681 acatcacccg ggcgcatttt ggcttttttc ccaccatcga tacacaacgt tgccatttct
     1741 gcttccagcg gcacaatgga actattagct ggcggcgttt gccagttaag ttttatctgc
     1801 aacatgtcag aaatgatatt ggcccgctgt gcttcttccg gagcacagaa actgatcgcc
     1861 agaccgctat ttcctgcacg agctgtacgg ccgatgcgat gtacatgaac ttcagggtcc
     1921 cacgccagct caaagttcac caccagctca agcgatttaa tatccagacc acgcgcagca
     1981 acatcagtcg caaccagtac acgggcgcta ccgttagcaa aacgtaccag ggtctgatcg
     2041 cgatcgcgtt gctccaaatc gccgtgtaat gacaatgcac tttgccctac ttcattcagc
     2101 gcgtcgcaga cagcctggca atcttttttg gtattgcaaa acaccacgca agaggatggc
     2161 tgatgcaagc ttaataaccg ttgcaacaga ggaattttgc ctttgctgga tgtctcataa
     2221 aattgttgtt caatgggtgg caaagcatct gttgagtcaa tttcaatcgc caaaggatcg
     2281 cgttgcactc gtccgctgat tacggcaatg gcttccggcc aggttgccga aaacagaagc
     2341 gtctgtcgag atgcaggcgc aaaacggatg acatcatcaa tggcatcgct aaatcccata
     2401 tccagcatgc ggtcggcctc atccatcacc aaagtattca gcgcatccag tgataccgtg
     2461 cctttttgca agtgatccag caaacgaccc ggcgttgcca cgataatatg cggcgcatgt
     2521 tgcaacgaat cacgctgcat accgaacggt tgaccaccgc acaacgtcaa aattttggta
     2581 tttggcagaa aacgcgccag ccgacgcaat tcacctgcca cctgatccgc cagttcacgc
     2641 gtaggacaca gcactaaagc ctgggtttga aatagcgacg catcaatttg ctgtaacaag
     2701 ccgaggccaa aagccgccgt tttgccgctg ccggttttcg cctgcacgcg aacatctttt
     2761 ccggcaagga tcgccggaag cgcggcggcc tgcaccggcg tcatagttaa ataacccaac
     2821 tcattaaggt tcgtgagttg ggcgggaggc aaaacattca gggtagaaaa agcggtcaca
     2881 atctattctc gtggtcatcg acgcaaagtt agcaggcgcg tatcctcgca gatctacgct
     2941 cacgatgcga caatttaatc ggttcttcat cgggtggtgg gtcaggcatg ggttgcgggc
     3001 gagggatcgg atcgggcact ggaacaggat cgccaggaat cggttcaggg acaggaattt
     3061 gcaaataaat aagtgtcgtc atatttccct ccggtcattg ggtggactct taaagggtag
     3121 acgctgataa ataacaggca aaaaaaagcc gactcatcaa agtcggcgtc gtacgaatca
     3181 attgtgctat gcagtaattc aaaaaaggaa gtaagacaat atggagcgca acgcccatcg
     3241 cttgacgttg cattcacctg caagggagat attgccctga atgggtagag agtttattga
     3301 cttcgctcaa actttgcggc gtttttgtat acagacagcc ggaaaaattg cttttgttac
     3361 aaccatttac tacggtgcaa ccataaagca acaccaccaa taagaacaac taacagaata
     3421 caaaaaattg aaaatccgaa ttgccacccg ccgccaggga tcccaccaag gttgacgcca
     3481 aataacccgg tcagaaaggt actgggtaaa aagaccattg ccatcaacga cattgtatag
     3541 gtacgacgag ctaaattttc ctgcatcacc tgagcgattt catccgccat cacgccagtc
     3601 cgtgctatac aggcgtcgat ttcgtcaagg ccgcgcccaa ggcgatcggc aatatcctgc
     3661 atccgacggc gttggtcatc gctcatccac ggcaaacgtt cactggcaag acgagcataa
     3721 acatcacgtt gcggtgccat atagcgacgc atcacaatta attgtttgcg cagcagagcc
     3781 aggaatccac gcggtggaat ttgctgatca aggagattat cttcaaggtc gataatttta
     3841 tcgtgcagct gctcgataaa ttcgctggaa tgatcggtca acgcatcgca cacatccacc
     3901 agccaccctc cgcaatcggt cggacccgtg ccctcttcca gatcgctcac cacatcgtcc
     3961 agcgccagca ctttgcgttg tcgggtcgaa acaattaacc gcccgtccat atatacacgc
     4021 atggcgacca gttgatcggg gcgttcatcg gtgctgccgt ttatacagcg caatgtaatc
     4081 agcgtgcctt caccgagacg gctgactcgg ggccgcgtgc tctcgcccgc cagcgcatca
     4141 cgtacgttat tgggaagcag cggtgttgtc gccagccatt gggcgctatc atggtgtaca
     4201 taattaaggt ggagccagca gggatgcgct tcatcaatca catctgtatt ttccagcggt
     4261 ttaacgccgc ctctaccatc cagcatccag gcaaatactg catccgggac attaacgtcc
     4321 gatcccttaa tcgcttccac agtgcctcca tcatcaacgc attattttgt agtctagcct
     4381 tctggccctg ttacgcaaca tctcatcacc ccattaccct gaaatgatta ataaaattct
     4441 gtctaaattg aatacaaaaa gcaaaatgct tttccgtata caaaccgtgt gaagtgttaa
     4501 atagcgtcta tcattatcag aattatctga tcatatgacg tggctttttt gcgatcggat
     4561 agcaacaaaa attgataaaa ataacgggat ctcaatgatt acgcacaact tcaataccct
     4621 ggacttactc accagtcctg tctggatcgt ttcgcccttt gaggaacagt taatttatgc
     4681 caatagcgcg gcgcgactgt tgatgcaaga cctcacgttt agtcagctac gaaccggtcc
     4741 ctattccgtc tcctcacaaa aagaactgcc gaaatacctc tccgatctgc aaaaccaaca
     4801 cgatattatc gaaatcctca ctgttcagcg taaagaagag gaaacagcat taaactgtcg
     4861 gcttgttttg cgaaagctga cagaagcaga accggtgatt attttcgaag gtatcgaagc
     4921 gtcggcaacg ctgggtttaa aagccagtcg ctcggcaaat tatcagcgca aaaaacaagg
     4981 tttttatgcg cgcttttttc tgactaactc tgcaccaatg ttgttgattg acccctcacg
     5041 agatggccaa atcgtcgatg ctaacctcgc cgcgctcaat ttctatggtt ataaccatga
     5101 aacgatgtgc cagaaacata cctgggaaat aaatatgctc gggcgtcgcg tcatgcctat
     5161 catgcatgaa atttcgcatt tacccggtgg tcataaacct ctgaatttta ttcataagct
     5221 ggcggatggt tcgactcgtc atgtgcagac ctacgccggg ccgattgaaa tttatggcga
     5281 caagctcatg ttatgtattg tacatgatat tactgagcaa aaacggctgg aggagcagct
     5341 ggaacatgct gcccaccatg acgcgatgac cggattactg aatcggcgac agttttatca
     5401 cattaccgag ccaggccaaa tgcagcatct cgccatcgcc caggattaca gcttgttgct
     5461 catcgacacc gatcgtttta aacacattaa cgatctctat gggcattcta aaggtgatga
     5521 ggtgttatgc gccctcgccc gcaccctcga aagttgcgct cgcaaaggcg atttggtgtt
     5581 tcgttgggga ggcgaagagt ttgtcttatt gctaccaaga accccactgg ataccgcgct
     5641 ttcgctggct gaaactatcc gcgtaagcgt ggcaaaagtg agtatttcgg gcttaccacg
     5701 ctttaccgtc agcattggtg tggcgcatca cgaaggaaat gaaagcatcg atgaactgtt
     5761 taaacgcgtt gatgatgctt tgtatcgggc gaaaaatgat ggacgcaacc gcgtgctggc
     5821 ggcataagcc gcggatgcgt ctcgagatca acgactgcgc ttagcgtggc gctcccagtt
     5881 ttcttgcttc gcctgcgccg ttttacgtag tgcgacgtaa cacgccccgc tgccgccatg
     5941 atgcggtagc gcagtgcaat atgcctgaac atcatcaaat tcggtcagcc agcgcgccac
     6001 atagctgcgg acaatattgg catgcgattt atcatcccgt cctttgccat gaataatcag
     6061 cacgttacgc aaaccatcag ccatggcttg ttgaatgaaa ctgaacatca ttttgcggca
     6121 ctcttccacc ggctggcgca aaagattcag gctcgcctgt tgcggatatt taccactgcg
     6181 cagcttatcc agcaccccat gttgcaaccc ttcccgccga aactccagcg gctgacttag
     6241 tgggatgatg tcgagaaatc cggtggtgag gaaattatca agctgcagcg tgtcgatacg
     6301 ctgcggcgca cgttggttac gcgttggatg ccagtggaca tcggtagcac gtttcagcgg
     6361 ctggacatct tccatggcgt caagaaacag cgatttgtcg tcaaggttca tgttacatcc
     6421 tcccgcaatt aagagcgcga tatgataacc agaccgggtc ggtccaacaa cgtattaccc
     6481 aaaattccag taataagttc caaatattgc cgatatttta agcaaaatgc ttatgcatga
     6541 ttattcattc acgatattaa taatgtaact tatattttcg tgaaatctgt cactgaagaa
     6601 aattggcaac taaaggttaa aaccgttata acacagtcac cggcgcagag gagacaatgc
     6661 cggatttaag acgcggatgc actgctgtgt gtactgtaga gtctggcgga tgtcgacaga
     6721 ctctattttt ttatgcagtt ttaactttgc agatagccgc attctcgccg gaattgcgtg
     6781 attaatgacg cggtcagtgg tgtctggcga ctatcacgcc gctgaatcaa ataataggcc
     6841 gctttcggta aaatttcgct aaccggcaac atcaccagcc cctgtccgtg caagggatcg
     6901 cagcccattt cttcaggcag tatgctgaga aaatcgcttt ttgccaccag actgatacag
     6961 gctgagaacg tctcgcagac tacaccgacc tgtggcgttt gcgcctgatc gtcaagcaat
     7021 tcactcaact gtttgtagta actgccgtgt ggcgtcggca ttgtccagct gtaatccagt
     7081 aactgtttga tcgaacgggc accaatggcg gggtgtcccg ggcggcaaaa gatcgcgaat
     7141 tgcttttcca gtaatttctc aaaagtaaat tcgtggtcgt acggtccctg ataataggta
     7201 ttgatggtga aatccaattc tccctgacgc aattcattaa tcatcgacac cagttgccct
     7261 tccataatgc gtacttttac ctgcggatgc tgctgatgaa aacgagatat gacagctggc
     7321 atcagactgc gggaaatact ggcccccatg ccgatattaa tctgccctgc cagttgccct
     7381 tgtcgttggc gaatatcctc ttgggctgcg cgcagctctt caagaataag actggcgtgc
     7441 tgataaaaac tttcaccggc atcagttaac gtcacgcctt tactacggcg aaaaaagagt
     7501 tgcgccgcta atccttcttc cagctcctga atagatttac tcagtgccgg ttgcgacata
     7561 ttcaacattc ggctcgctcc gcgaatgctg ccctgacgag ccacttcaac aaaagcccga
     7621 atttgatgaa tttttacctg aaaagccatg acgccaccga taaccgttat ttatcagacc
     7681 aaagaaactg gcatctactt taatgcagat gattgtgtca gggtaattta tgaacggtta
     7741 aaactgtgaa aaatcagtta gtgataagta aaaactatcg ctacgtgaac cgggtcacac
     7801 tttttactga tgacgggaaa ggttatggag tctttgaatc aatttgttaa ttcgcttgcc
     7861 ccaaaattat cgcactggcg acgtgatttt catcactatg cagagtctgg ctgggtggaa
     7921 ttccgcactg ccacccttgt tgcggaagaa ttgcagcagc tcggctattc actggcgctg
     7981 ggccgcgaag tagttaatga aagtagccgg atgggactac ctgatgaact cactctacaa
     8041 cgcgaattcg agcgcgctcg tcaacagggt gcgctagcac aatggattgc ggtttttgaa
     8101 ggtggtttca ctggtatcgt cgccaccctg gataccggtc gccccggtcc ggtgatggct
     8161 ttccgtgtcg atatggacgc gctggatctc agtgaagagc aggatgtcag ccatcgcccc
     8221 taccgcgacg gttttgcgtc atgtaacgcc ggaatgatgc atgcctgtgg tcatgatgga
     8281 cataccgcca ttgggcttgg gctggcgcat acccttaaac aattcgagtc cggactacat
     8341 ggcgtcatca aactgatttt tcagcctgca gaggaaggta cgcgtggcgc gcgggcgatg
     8401 gtcgatgcag gtgtcgtaga tgatgttgat tattttactg ccgtgcacat tggcactggc
     8461 gtacctgcgg gcaccgtggt gtgcggcagt gataatttta tggcaaccac caaatttgac
     8521 gcgcacttca ccggtaccgc cgctcacgca ggcgcaaaac cagaagacgg tcacaatgcc
     8581 ttgttggcgg cagcacaagc cactcttgca ctgcatgcaa tcgccccgca cagcgaagga
     8641 gcttccagag taaacgtggg cgttatgcag gcaggaagcg gtcgtaacgt tgttcctacc
     8701 tcggcgttgc tgaaagtgga aacacgcggg gccagcgacg tcattaatca atatgttttt
     8761 gaccgtgcac aacaagcgat tcagggcgca gcaaccatgt atggtgtcgg cgttgaaact
     8821 cgtctgatgg gtgcagttac cgccagttct ccttcgccgc aatgggtcgc atggttgcaa
     8881 agtcaggcgg ctcaggtcgc gggggtcaat caggccattg aacgtgttga agcgcctgcg
     8941 ggttccgaag atgccacatt aatgatggcc cgcgtgcagc aacatcaagg gcaagcctcc
     9001 tacgtggtgt ttggcacaca gctggcggca ggtcatcaca acgaaaaatt cgattttgac
     9061 gagcaggttc tcgctattgc cgtcgaaacg ctggcgcgca ccgcgctcaa ttttccctgg
     9121 acgcgaggta tctgatgcag gaaatctatc gttttatcga cgatgcgatt gaagccgatc
     9181 gccaacgtta taccgatatt gccgatcaaa tctgggatca tccagaaaca cgttttgaag
     9241 agttctggtc agcggagcat ctggcttcgg ctctggaatc tgcaggcttc accgttaccc
     9301 gcaacgtagg caatatccca aatgccttta ttgcttcgtt tggtcaaggc aaaccggtta
     9361 tcgccctgct gggagaatat gacgccctgg caggtttaag tcagcaagct ggttgcgcgc
     9421 aacctacatc cgcgacgccc ggtgaaaatg gtcacggttg cggacacaat ttgcggggaa
     9481 ccgccgcctt tgccgctgca atagccgtca agaaatggct ggaacaatat gggcaaggcg
     9541 gcacagtgcg cttttatggt tgtcctggcg aagaaggcgg ctcgggtaaa acgttcatgg
     9601 tccgcgaggg ggtatttgat gatgtggatg cggcactcac ctggcacccg gaagcctttg
     9661 ccggtatgtt caatacccgc acgctggcaa acattcaggc atcatggcgc tttaaaggga
     9721 tcgcagcaca tgccgcgaat tcccctcatt tgggacgcag cgcccttgat gccgtaacgt
     9781 tgatgaccac tggcaccaac ttcctcaacg aacatattat tgaaaaagcg cgcgtacact
     9841 atgccatcac aaatagcggc gggatctcgc ccaacgtggt ccaggcgcag gcagaagtgc
     9901 tttatcttat ccgcgccccc gaaatgaccg acgtgcagca tatttatgat cgggtcgcca
     9961 aaatcgccga aggtgcggca ttgatgaccg aaaccacggt tgaatgccgc ttcgacaaag
    10021 cctgttccag ttatctcccg aatcgcacct tagaaaatgc catgtaccat gccctatccc
    10081 attttggtac cccggaatgg aactccgaag aactggcttt tgcgaaacaa attcaggcta
    10141 cgctcacccc caacgatcgg caaaacagtc tgaataatat cgccgcaacc ggtggcgaaa
    10201 acggcaaggc ttttgcacta cgtcatcgtg aaacggtact ggcgaatgag gtcgctccat
    10261 atgccgccac cgataacgtg cttgcggcat cgactgatgt cggcgacgtt agttggaaac
    10321 tgcctgttgc ccagtgtttc agcccctgtt ttgccgtcgg tacaccgcta catacgtggc
    10381 aactggttag ccaggggcga acatctattg ctcataaagg aatgctgctg gcggcgaaaa
    10441 ctatggcagc aaccacagtc aatctcttca ttgattcagg gctattgcaa gaatgccaac
    10501 aagagcatca gcaagtaacg gacacgcaac cgtatcactg ccctatcccg aaaaacgtga
    10561 caccgtcacc tttaaaataa caacaacaac gcaaacacaa caaccgagga atgcccatga
    10621 gtatgtcatc cataccgtcg tcctcccaat ccgggaagct ctatggctgg gtcgaaagaa
    10681 ttggtaacaa ggttctccat ccttttttgc tctttatcta tttgattatc gtactcatgg
    10741 tgacgacggc aattttgtcg gcctttggcg tcagtgcgaa aaacccgacc gatggaacgc
    10801 cggtggtggt gaaaaacctg ctcagtgtgg aaggattaca ctggtcttta cccaatgtta
    10861 ttaaaaactt tagcggtttt gctccacttg gtgcgatcct ggcgctggtt ttaggtgccg
    10921 gtctggcgga gcgcgtcggc ttactgccag cgctaatggt taaaatggca tcgcatgtta
    10981 atgcccgcta cgccagttat atggtgctgt ttattgcttt tttcagccac atttcttccg
    11041 atgcgacgtt agtgatcatg ctaccgatgg gtgcgctgat ttttctggcg gtgggcaggc
    11101 atccagttgc aggtttactg gctgccattg caggcgtagg ttgcggcttt acggctaatt
    11161 tactgattgt cacaaccgac gtgttgctgt cggggatcag cacggaagcg gcggctgcgt
    11221 tcaat
//
LOCUS       DAJTYZ010000128        11196 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000128 DAJTYZ010000000
VERSION     DAJTYZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11196
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.128"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..651
                     /locus_tag="OO596_003294"
     CDS             <1..651
                     /locus_tag="OO596_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HCR6719076.1"
                     /translation="DIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAF
                     EEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAV
                     SFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDC
                     KIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            821..1099
                     /locus_tag="OO596_003295"
     CDS             821..1099
                     /locus_tag="OO596_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6719077.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            1099..1476
                     /locus_tag="OO596_003296"
     CDS             1099..1476
                     /locus_tag="OO596_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719078.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            1945..3081
                     /locus_tag="OO596_003297"
     CDS             1945..3081
                     /locus_tag="OO596_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6719079.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(3123..3893)
                     /gene="yafV"
                     /locus_tag="OO596_003298"
     CDS             complement(3123..3893)
                     /gene="yafV"
                     /locus_tag="OO596_003298"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6719080.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            4074..4520
                     /gene="ivy"
                     /locus_tag="OO596_003299"
     CDS             4074..4520
                     /gene="ivy"
                     /locus_tag="OO596_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6719081.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(4563..7007)
                     /gene="fadE"
                     /locus_tag="OO596_003300"
     CDS             complement(4563..7007)
                     /gene="fadE"
                     /locus_tag="OO596_003300"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6719082.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            7247..7825
                     /gene="lpcA"
                     /locus_tag="OO596_003301"
     CDS             7247..7825
                     /gene="lpcA"
                     /locus_tag="OO596_003301"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6719083.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            8031..8798
                     /locus_tag="OO596_003302"
     CDS             8031..8798
                     /locus_tag="OO596_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6719084.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(8769..9509)
                     /locus_tag="OO596_003303"
     CDS             complement(8769..9509)
                     /locus_tag="OO596_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6719085.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(9665..9943)
                     /gene="yafQ"
                     /locus_tag="OO596_003304"
     CDS             complement(9665..9943)
                     /gene="yafQ"
                     /locus_tag="OO596_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCR6719086.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(9946..10206)
                     /gene="dinJ"
                     /locus_tag="OO596_003305"
     CDS             complement(9946..10206)
                     /gene="dinJ"
                     /locus_tag="OO596_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HCR6719087.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            10392..11165
                     /locus_tag="OO596_003306"
     CDS             10392..11165
                     /locus_tag="OO596_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6719088.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         3003 a         2650 c         2791 g         2752 t
ORIGIN      
        1 gatattaaag gaaaaatcat cacaactgat gcgatgggtt gccagaaaga tattgcagag
       61 aagatacaaa aacagggagg tgattattta ttcgcggtaa aaggaaacca ggggcggcta
      121 aataaagcct ttgaggaaaa atttccgctg aaagaattaa ataatccaga gcatgacagt
      181 tacgcaatga gtgaaaagag tcacggcaga gaagaaatcc gtcttcatat tgtttgcgat
      241 gtccctgatg aacttattga tttcacgttt gaatggaaag gactgaagaa attatgcgtg
      301 gcagtctcct ttcggtcaat aatagcagaa caaaagaaag agccagaaat gacggtcaga
      361 tattatatca gttctgctga tttaaccgca gaaaagttcg ccacagcaat ccgaaaccac
      421 tggcacgtgg agaataagct gcactggcgt ctggacgtgg taatgaatga agacgactgc
      481 aaaataagaa gaggaaacgc cgcagaatta ttttcaggga tacggcacat cgctattaat
      541 attttaacga atgataaggt attcaaggca gggttaagac gtaagatgcg aaaagcagcc
      601 atggatagaa actatctcgc gtcagtcctt gcggggagcg ggctttcgta atctttccct
      661 agggagtcag ccgaatgttg ggaaggattt gacggatgaa gagaagaact gcttggccgg
      721 cggcagtacg ggaagcccgg gaggatgggg accagaagat gagaatagcg caagggatcg
      781 tatgcagcat gaacggtata aagatgaatt gcgacagaaa atgggaaaac ccgacgttac
      841 agattcaaat ttgaaaaata ttatagacga tctttatcgg ccaaatgcaa aagtaggaag
      901 tggaagcaca gcagatgctg ttcgttatga attggccaca ggtgaaaaag ttggtggtag
      961 aggacatgta ttgaaagcac aggattatac aagggcttta caggattggt tggataagaa
     1021 tccaacagca tctccagggg atcgagcagc agcagaaaac gttcttcagg atttacagaa
     1081 tgctttggga ggcaagtaat gaaccaatat attgctagtt atatagaaaa aatggcatca
     1141 ttttccactg ttttgtctga gacagtctca ttagctaatg aatactggca tccagaaacg
     1201 ccaccggtgg ttatgttgtt ctctcaggtt ggaaaaacga ttgtaaaaaa tttttatgaa
     1261 atgggggata ttgaaagaga aaaaatattt aaacatgtcg aagatggtat ggtctcgctt
     1321 gatgatgaat tggccacagt tgtagctaca gggcttatag aggcaatagt cactgcgacc
     1381 gatagcaatt ctttactttg gcaagattta agtaaatttc tgggtgacaa atcaaagtct
     1441 catgcattag tgtggaaaaa ttttggtcag gagtgatatt attcaagaag tattaagctc
     1501 gctattgaga tcttaatgct tcttgagatt aaattaaagt taggacgaag taattgcagg
     1561 tggaaataaa tccatagtga cgtgtggcaa tggctattag atgttatggt gtgatgggct
     1621 ttggtgattt aagagctaag ttagattgcc ttatgaaaaa gtatggttat atatttaatg
     1681 atgtgtatca tatatttaaa taaaaaacgg ttctctcttg tcaagatagt aataggatag
     1741 actattgtgt tgataaatat aattaagagt ctactttgtt gattttagaa gactttggag
     1801 cagggaaaga tcatcggaag tacacttttt gtactaaata attcgcgcta aatgttcaat
     1861 aattgagata ttccttatta cctaaaacta tttttcatag cttatatatg atcaaatact
     1921 ccttgcataa ataaggagaa caaaatggaa cttaaaaaac tgatggaaca tatttctatt
     1981 atccccgatt acagacaagc ctggaaagta gaacataaat tgtcagatat tctactgttg
     2041 actatttgtg ccgtcatttc tggtgcagag ggttgggaag atatagagga ttttggtgaa
     2101 acacatctcg attttttgaa gcaatatggt gattttgaaa atggtattcc tgttcacgat
     2161 actattgcca gagttgtatc ctgtatcagt cctgcaaaat ttcacgagtg ctttattaac
     2221 tggatgcgtg actgccattc ttcagatgat aaagacgtca ttgcaattga tggaaaaacg
     2281 ctccggcact cttatgacaa gagtcgccgc aagggagcga ttcatgtcat tagtgcgttc
     2341 tcaacaatgc acagtctggt tctcgggcag attaagacgg atgagaaatc caatgagatt
     2401 acagccattc ctgaacttct taacatgctg gatattaaag gaaaaatcat cacaactgat
     2461 gcgatgggat gccagaaaga tattgcagag aagatacaaa aacagggcgg tgattattta
     2521 ttcgcggtaa aaggaaacca ggggcggctt aataaagcct ttgaggaaaa atttccgctg
     2581 aaagaattaa ataatccaga gcatgacagt tacgcaatca gtgaaaagag tcacggcaga
     2641 gaagaaatcc gtcttcatat tgtttgcgat gtccctgatg aacttattga tttcacgttt
     2701 gaatggaaag ggctgaagaa attatgcgtg gcagtctcct ttcggtccat aatagcagaa
     2761 caaaagaaag agccagaaat gacggtcaga tattatatca gttctgctga tttaaccgca
     2821 gagaagttcg ccacagcaat ccgaaaccac tggcacgtgg agaataagct gcactggcgt
     2881 ctggacgtgg taatgaatga agacgactgc aaaataagaa gaggaaatgc agcagaatta
     2941 ttttcaggga tacggcacat cgctattaat attttgacga atgataaggt attcaaggca
     3001 gggttaagac gtaagatgcg aaaagcagct atggacagaa actatctggc gtcagtcctt
     3061 gcggagagcg ggctttcgta gtcttacccc gactctcccc cagccttaaa cacaaccccc
     3121 actcaccgca acctaaactc atccgcatcc tgccatgccg gaaacttttc tctatattcc
     3181 cgcaatgcca tcatcgacag ctccgcatca atgcgcgttg cctgatgcgc gtcggcagta
     3241 gcgataatct cgccttgtgg attaatcacc cggctgtcac cgcgataatg gcagccgttg
     3301 ccatcgctgc cgacgcgatt gcatcccgcc acatacgcct gattctcaat cgcgcgggcc
     3361 gtcagcaatg cctgccagtg cagagagcgc ggagcaggcc agttggcaac gtagatggcg
     3421 aggtcataat cgttgagatt gcgcgaccac accggaaaac gtaagtcgta gcacaccaac
     3481 ggcaaaatac gccagccgcg ccattccaca atcactcgcg cattgcccgc tttataatgt
     3541 agatgctcat ctgccatgcg gaacagatga cgcttatcat aaaaatgtac cgtgccgccc
     3601 ggctcaacca gcaaaaagcg gttaaccgaa ccagactccg tttgtaatgc aacactgcct
     3661 gcaatcagcg cattgcactg ctgcgcctta gctgtcatcc agtccactac gtcatcttgt
     3721 gctagcgacg aagctgccgc ttccatggca aagccgctgg taaacatctt cggtagaacg
     3781 atcacatcgc gcccggtaat accttccagt tgacgatcaa aatgacgcag gttggcaggg
     3841 ccatccatcc acaccagtgg ttgctgcaaa agcgtaatct tcaaaccagg cacggtgtac
     3901 aactccttta tgcgaagggt tttataactt taacacctta tcaggcagtt gccttagcgc
     3961 agaataaatt gataacaaat gctgatattg gaaatatctg atttgcaaat tatcgtgtta
     4021 tcgccaggct ttagaaggtt aataacatgg gcaggataag ctcgggagga atgatgttta
     4081 agacaataac gacagtcgcc gctctggtca tcgccaccag tgcaatggcg caggatgatt
     4141 taaccattag cagccttgca aagggcgaaa ccaccaaagc tgcatttaat cagatggtac
     4201 aagggcataa gctgcctgcc tgggtgatga aaggcggtac ttatactccc gcacaaaccg
     4261 taacgttggg agatgatacg tatcaggtga tgagcgcgtg caaaccgcat gactgtggct
     4321 cgcaacgtat cgctgtgatg tggtccgaga aatctaatca gatgacgggg ctgttctcga
     4381 ctattgatga gaaaacgtcg caagagaaac tcacctggct gaatgtgaac gatgcgcttt
     4441 cgattgatgg taaaacggtg ctgttcgcgg cgttgaccgg cagcctggaa aaccatccgg
     4501 atggctttaa ttttaaataa ttagcggata aagaaacgga gcctttcggc tccgttattc
     4561 aattacgcgg cttcaacttt ccgcactttc tccggcaact ttaccggctt cgtcgccagc
     4621 tcttccggat caaagtcatc aacgttaata ctgcgcagac ggctttcttc agctttcacc
     4681 agaatagcgg cttcatcttt atcaatcagc cccttcgcca gcgcgttgtg cgccagttca
     4741 tccagacggg taaacggcag gtttttaccc agctctttac agatccgctg atgaattggg
     4801 tcggcggcaa tcacatccac cagcgcctct tccagcaagc caaccggatt atgctcgctc
     4861 ggcgtcaggt actgaccgcg accaatgcgg gaacgggtgg cgttcggcac ttgtaaaatc
     4921 ttcgccactt tatgatccag cttgtcagaa ggtgccagat aatgacgtcc ggtcgggaag
     4981 atcaccacat tcagcagccc ggcaaccacg cggttcggga agttttgcag taaatcatcc
     5041 atcgcctgtt cagcctgata cagcgcatct tgtacgcccc agtgcaccag cggcaggtcg
     5101 gcttcattac ggccttcgtc gtcataacgc ttcagcacgg cagaggcgag gtagagctgg
     5161 cttaaaatat cccccagacg ggccgagatg cgctcgcgac gtttcaggct gccgtccagc
     5221 actgccatcg agacatcaga aagcagggcg aggttggcgc tcaggcggtt caggtgctga
     5281 tagtagcgtt tagtggcatc gccggttggc gtgctgctgg ttaaaccgcg cgtcaggccc
     5341 agccagaagc tgcgaacttt gttgctaccg acgtgaccga tatgtttgaa caacagttta
     5401 tcgaacgcgt tgacgtcatt gttcttcgcc gcttccatct cttccagcac gtacggatgg
     5461 caacgaatcg ctccttgtcc gaagatcatc atgctgcggg tcagaatgtt agccccttca
     5521 acggtgatgg caatcggtgc gccctggtaa gcacgcgcca ggaagttgct ttgcccgagc
     5581 ataatgcctt tgccaccagt gatatccatc gcatcaataa tcgactgctg cccgcggtgg
     5641 gtacagtgat acttaacgat agccgacagc acggcaggtt tttcgccgag cataatgccg
     5701 taggtaatca gcgatgccgc agcatccatc acgtaggcat taccggcaat acgcgccagc
     5761 ggctcttcaa tcccttccat cttaccgata gagattttga actgacggcg aatgtgagca
     5821 tacgcgcccg ttgccagcgc taccgatttc acgccgccgg ttgagttgga aggcagggtg
     5881 atgccgcggc ctaccgagag gcactccacc agcatccgcc agccttgccc ggccattttc
     5941 ggcccaccga tgatgtaatc gatcggtacg aagacatctt taccgcgcgt cggtccgttc
     6001 tggaacggta cgttcagcgg gaagtggcga cgaccaattt ccacgcccgg cgtggtggtc
     6061 gggatcagcg cacaggtaat acctaaatct tctgcgccac ccagcagctt ttctgggtcg
     6121 gagagtttaa acgccagccc aagcacagtc gcaatcggtg ccagcgtaat atagcgtttg
     6181 ttccaggtca gacgcatccc cagcacctgc tggccctgcc attcgcccat gcagacaatc
     6241 ccggtgtccg gaatcgcgcc cgcatcggaa cccgcttccg ggcttgtcag tgcaaagcaa
     6301 gggatctcct gaccacgcgc cagacgcggc agatagtgat ctttctgctc gtcagtgccg
     6361 taatgttgca gcagctcgcc cgggcctaat gagttcggca cgccgacggt aatcgccagg
     6421 atcccgctca cgccggagag tttttgcagc acgcgagact gggcataagc tgagaactcc
     6481 agtccgccgt actctttttt gataatcatc gcgaagaagc gatgctcttt aaggtacgcc
     6541 cacaactccg gcggcagatc cgccagctca tgagtaatct ggaaatcatt cgccatccgg
     6601 caggcttctt ctaccgggcc gtcgagaaac gcttgctctt cggcggtcag gcgcggctgc
     6661 ggatagttat gcagcttttt ccagtccggc ttgccctgga acaagtcgcc ctcccaccag
     6721 gtggtgcccg catcaatcgc ttctttctca gtgcgcgaca tcggcggcat caccttacgg
     6781 aaaccgcgaa ataccggcgc ggaaatcatc gacttacgca taggcgcaaa gttaaatggc
     6841 acgaggataa tggccagagg caccagtacc cacgctgacc acagaccagc aacgccgagg
     6901 gcggctgtcc aggcgagcaa aatcagactg ctgataaata agctcacgcg gtgatagaac
     6961 aacgcgccga gcaggacaac cgtagcgaga atactcaaaa tcatcataac gaaaagcccc
     7021 ttacttgtag gaggtctgac cacttgtgat gatatggttg tagtggatgt aaaaacattt
     7081 agcaatgtgt ttacaatata attacaacaa agctcacatt gttgctgttt ttatccgcac
     7141 ttcaggtcaa aaagtcctgg tcatagcacc tgcccgtact tctcgctttt ggcggtatcc
     7201 ggtacactgc attttgtcta ttacatttat gctgaaggat atcctcatgt accaggatct
     7261 tattcgtaac gaactgaacg aagcggcgga aacgctggct aactttttaa aagatgacgc
     7321 caatattcac gccattcagc gcgcggcggt cctgttagca gacagcttta aagccggtgg
     7381 caaagtgctt tcctgcggca acggcggttc ccattgcgac gctatgcact ttgccgaaga
     7441 gttgaccggt cgctaccgtg aaaaccgtcc gggctacccg gcgattgcta tttctgacgt
     7501 tagtcatatt tcctgcgtcg gtaatgattt cggtttcaat gatattttct cccgctacgt
     7561 tgaagcggta ggtcgcgaag gcgatgtact gctggggatc tccacctccg gtaactctgc
     7621 aaacgtgatc aaagcgatcg cagcggcgcg tgagaagggg atgaaagtga tcaccctgac
     7681 cggtaaagac ggcggtaaaa tggctggcac ggcggatatc gaaattcgcg ttccgcactt
     7741 tggttatgcc gaccgcattc aggagattca cattaaagtg atccatatcc tgatccagtt
     7801 aattgaaaaa gagatggtta agtaagtctg gcgtaggccg gataaggcgt ttacgccgca
     7861 tccggcactt gtgcgctgat gcctgatgcg acgctgatgc gtcttatcat gcctacaaat
     7921 ctgtacgcga accgtaggcc gaataagacg tttacgccgc atccggcctg aaaattctta
     7981 aatcaatctt cgccgggggc catgcgctcc cgctgttgtg gaggttaccc atgtgcgaat
     8041 tgctcgggat gagcgccaac gtccctaccg atatctgctt tagtttcacc ggtctggtgc
     8101 agcgtggtgg tggaaccggg ccacataaag atggctgggg cattaccttt tacgaaggta
     8161 aaggctgtcg cacatttaaa gatccacaac ccagctttaa ttcccccatc gccaaacttg
     8221 tccaggacta cccgataaaa tcctgttcgg tggtggctca tattcgccag gccaatcggg
     8281 gcgaggtggc gctggaaaat actcacccgt ttacccgtga gttatggggg cgtaactgga
     8341 cttatgccca taacggacaa ctgacgggct acaaatcact ggaaaccggc aacttccgcc
     8401 cggtcggtga aaccgatagc gaaaaagcct tctgctggct cctgcataaa ttaacgcagc
     8461 gttacccgcg cacaccgggc aacatggcgg cagtgtttaa atatatcgcc tcactggcgg
     8521 atgaactgcg gcagaagggc gttttcaaca tgctgctttc tgacgggcgc tatgtgatgg
     8581 cgtattgctc gactaattta cactggatca cccgccgcgc gccgtttggc gtggcaacgc
     8641 tgctggatca ggatgtggaa atcgacttca gctcgcagac cacaccgaat gatgtggtca
     8701 cggtgattgc aacacagccg ctgacgggca atgaaacctg gcaaaagatt atgccaggcg
     8761 aatggcgctt attttgcctc ggggagcgtg tagtttgatg ccagttgtgg ctgcacaact
     8821 tcgtggctta acggcttgct gaccacgtaa cgaccattga ccacagaaac ggtcggtggc
     8881 ttacgggttt gctcaaagta gtcgtaaccc ggcttcagtt gctcccagaa gtccttaaag
     8941 ttggaatatt tatggcgctt catattggcg tcggtcatgc ggaacgggta aatactcact
     9001 tgcacgctcg gctgaccaaa caccagcgca ccagtaacga actggaagat ctcatcaata
     9061 ccctgattgg tcattgcgta gcagccgatg gaaacacaat cgccgtggat catcaggtat
     9121 ttcccttcat aaccatgcgc acggtcatag gcattgggga aaccaatatt aatcgctttg
     9181 tagtaacggc tgtctggttt taactgatta cgctggacgc tataaaaccc ttccgggctt
     9241 ttgaaatcgc cctgacgctg ttttggccct aagccgccgg aatatttaca gattttataa
     9301 ctgtcgagca gctgatattg ctcgcccatt ttgacgtaga gatcgagcgt acgttcttcc
     9361 ttgaagatct ggatgtagac aggggatccc atcaactgct gcttatactc tttgctcact
     9421 ggcgtggtcg agctactgct gcccagcaaa ccggcaaacg aaacgcacgg gatcaacaac
     9481 atcgcaagaa ttaatgcgat tttacgcata ctgcttattc cttgataaaa cggttacaca
     9541 cgccaggacg gcaaaataga tcccaaatcg gaatagtctg gatttggaag gctcacatta
     9601 tcaccaaaag agttttacgc aagcctgtcg gcgcagggtt tacaaatttc atcggcagcg
     9661 tgctttaccc aaagagcgcc gcgtgagttc cagttctctc aaatcgtaaa agtttatcgg
     9721 taagtttgta aatcaggatc cagtccggtt cgacatgagc atcgcgataa cctttccatg
     9781 aaccttgcag cgggtggtct ttataaacag ctggaagcgg taaagcatta ttgataagaa
     9841 gcgtcataag atatttcaat ttattcatat ccttatgacg cttttgtgca agttttacat
     9901 cctttgaaaa ttgtcccgag tattcaatat ccctttgaat catatttaaa ttcctaattt
     9961 atcaaataaa tcatcagcgt ctttggcctt atgaacatca acgccagctt cgctgttttt
    10021 gattgattga atggttaatt gattaggctc gcgtaaatca aacggcaatg ccttttcacg
    10081 cgcgaccttt gtgagggtta tgcgaaccag gtcagagatg gtcagcccca tcccggccag
    10141 tacgtccgcc gcctgattct tcagatcttc atcgattcgg gcgcgaacaa acgcgttagc
    10201 agccattggg ggatctcctg tgctgtatat ttattcagct tgaattgtag ctcaaatgag
    10261 caacagcgac aatccatgag taatttaata aacggatgtt tttacataat aaactgaaat
    10321 atatttatta attttataat aatggtttat taagtttgtt caggtacggg ccaggataca
    10381 ctgcaacatc aatgcatgac cagccattat tgagaatgtc tttgccgtcg attccctctt
    10441 ttgtgttgtc gggattactg ttgctttgct tgccgttttc ttcatttgcc tgcgccacca
    10501 catcacatat ctctttcagc tacgccgccc gccagcggat gcaaaaccgt gcgcgtttat
    10561 taaaacagta ccaaacccat ctgaaaaaac aggccagcta cattgtggaa ggcaatgccc
    10621 aaagcagaag ggcgctgcgc cagcacaacc gggagcagat aaaacagcat ccagaatggt
    10681 ttcctgcgcc gctcaaggcg agtgacagac gctggcaggc gctggcggaa aacaaccact
    10741 ttttaagcag cgatcacctg cataacatca ccgaagtggc gattcaccgt ctggagcagc
    10801 agcttggcaa gccttacgtc tggggcggca cgcggcccga tcaaggcttc gattgtagtg
    10861 ggttagtttt ttatgcctac aacaagatcc tcgaggcgaa gctcccgcgt acggccaatg
    10921 agatgtatca ctaccgtcgg gcaaccattg tggcgaataa cgacctgcgc cggggagatt
    10981 tgctgttttt ccatatccac agccgcgaga ttgccgacca tatgggggtg tatctgggcg
    11041 acggtcagtt tattgagtca ccgcgcaccg gggaaaccat tcgggtaagt cggttagctg
    11101 aacctttctg gcaggatcat tttttgggcg cgcggcggat attgacggaa gagacgattt
    11161 tgtaggacgg ataaggcgtt tacgccgcat ccggcg
//
LOCUS       DAJTYZ010000129        11142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000129 DAJTYZ010000000
VERSION     DAJTYZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.129"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1225
                     /gene="narU"
                     /locus_tag="OO596_003307"
     CDS             <1..1225
                     /gene="narU"
                     /locus_tag="OO596_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6719089.1"
                     /translation="VTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGG
                     RRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFP
                     KAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAW
                     IWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSA
                     GFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSA
                     LLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSD
                     EQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCV
                     LLTWLVYGRRKFSQK"
     gene            1307..5047
                     /gene="narZ"
                     /locus_tag="OO596_003308"
     CDS             1307..5047
                     /gene="narZ"
                     /locus_tag="OO596_003308"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCR6719090.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFHSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5044..6588
                     /gene="narH"
                     /locus_tag="OO596_003309"
     CDS             5044..6588
                     /gene="narH"
                     /locus_tag="OO596_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6719091.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6588..7283
                     /gene="narW"
                     /locus_tag="OO596_003310"
     CDS             6588..7283
                     /gene="narW"
                     /locus_tag="OO596_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6719092.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7280..7960
                     /gene="narI"
                     /locus_tag="OO596_003311"
     CDS             7280..7960
                     /gene="narI"
                     /locus_tag="OO596_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6719093.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8039..8932
                     /locus_tag="OO596_003312"
     CDS             8039..8932
                     /locus_tag="OO596_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCR6719094.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9028..9873)
                     /gene="nhoA"
                     /locus_tag="OO596_003313"
     CDS             complement(9028..9873)
                     /gene="nhoA"
                     /locus_tag="OO596_003313"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCR6719095.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10046..10615
                     /locus_tag="OO596_003314"
     CDS             10046..10615
                     /locus_tag="OO596_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR6719096.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10619..10846)
                     /gene="pptA"
                     /locus_tag="OO596_003315"
     CDS             complement(10619..10846)
                     /gene="pptA"
                     /locus_tag="OO596_003315"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCR6719097.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(10946..>11142)
                     /locus_tag="OO596_003316"
     CDS             complement(10946..>11142)
                     /locus_tag="OO596_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6719098.1"
                     /translation="VVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMGRNYLASVLTGSGLS"
BASE COUNT         2591 a         2907 c         2974 g         2670 t
ORIGIN      
        1 agttaccgtt aatctcaata aaatcggttt taattttact accgatcaac tctttttatt
       61 aaccgcatta ccctccgttt ctggcgcatt attgcgtgtt ccctactcct ttatggtgcc
      121 tatattcggt ggacgccgat ggacggtttt tagtactgca atcctgatta ttccttgcgt
      181 ctggctcgga attgccgtgc aaaatccgaa tactcctttt gggatattta tcgttatcgc
      241 tttgctatgc ggttttgcag gtgcaaactt tgcttcgagc atgggcaata tcagtttctt
      301 ctttccaaaa gccaaacaag ggagcgctct tgggattaat ggcggattag gaaacttagg
      361 tgtaagtgta atgcagctgg ttgcaccgct ggtcattttt gtacctgtat ttgcctttct
      421 cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg tcgctggcga atgccgcatg
      481 gatttgggta ccgctactgg cgattgccac gatcgccgcc tggtcaggga tgaatgatat
      541 cgccagttca cgcgcctcaa ttgacgacca gctccctgtc ttacaacgcc tgcatctctg
      601 gctgctgagc ctgctttacc ttgccacctt cggttcgttt atcggttttt ctgcgggttt
      661 tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg
      721 cccatttatc ggtgccatcg cgcggtcggt tggtggtgct atttccgata aattcggcgg
      781 cgtgcgggtg acgttgatca actttatttt tatggcgatt ttcagtgccc tgctgttcct
      841 taccttaccg ggcacaggct ccggtaattt catcgccttt tacgccgtat ttatggggct
      901 gtttctgact gcgggtctgg gaagtggttc tactttccag atgatcgccg tcatctttcg
      961 ccagataacc atttatcggg tgaagatgaa aggcggtagt gatgagcaag ctcagaaaga
     1021 agccatcacc gaaacggcgg cggctctggg ctttatctca gccattggcg cagtgggcgg
     1081 cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg accggctctc cggtcggcgc
     1141 gatgaaagtg tttttaatct tctacatcgt ttgtgtgctg ctgacctggc tggtttatgg
     1201 tcggcggaag ttcagccaaa aataagagat cgatgcctga tgtacaaccg tgtcgcatcc
     1261 gacaaggcat cagatcccaa ttcattcctg gagcaggagt catgtcatga gtaaactttt
     1321 ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt gccgatggtc acggacaggt
     1381 gatgcatagc aaccgcgact gggaggacag ctatcgccag cgttggcagt tcgacaaaat
     1441 tgtgcgttcc acccacggtg ttaactgtac aggctcctgt agctggaaaa tctacgttaa
     1501 aaatggtctg gtgacctggg aaatccaaca gaccgactac ccgcgcactc gccctgacca
     1561 gcccaatcat gaacctcgcg gctgcccgcg tggcgcaagt tactcctggt atctctacag
     1621 cgctaaccgc ctgaaatacc cgctcattcg taaacgactg attgaactgt ggcgtgaagc
     1681 tctcaagcaa cacagcgatc cggtactggc gtgggcatcg attatgaacg atccgcaaaa
     1741 gtgcttaagc tacaaacaag tgcgtgggcg cggcgggttt atccgctcca actggcagga
     1801 acttaaccaa ctgattgccg ccgctaacgt ctggaccatc aaaatctatg gtccggatcg
     1861 cgttgccggt ttctcgccga tcccggcgat gtcgatggtg tcatacgctg ccggaacgcg
     1921 ttatctgtcg ctgttaggcg gcacctgttt aagcttctac gactggtatt gcgacctgcc
     1981 gcccgcctcg ccgatgacct ggggcgaaca aaccgacgtg ccggaatctg ccgactggta
     2041 taactccagt tacatcatcg cctgggggtc taacgtaccg cagacacgta cgccggacgc
     2101 ccacttcttt accgaagtac gttacaaagg caccaaaact atcgccatta cccctgacta
     2161 ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg aaacaaggca ctgatagcgc
     2221 cctggcgatg gcaatgggcc atgtgatttt aaaagagttt catctcgata atcccagcga
     2281 ctactttatc aactactgcc gccgctatag cgacatgccg atgctggtaa tgctggaacc
     2341 tcgcgacgat ggtagctacg tacccgggcg catgatccgc gcgtctgacc tggtagatgg
     2401 actaggcgaa agcaataatc cgcagtggaa aaccgtggcg gttaataccg caggtgaatt
     2461 ggtagtgccg aatggttcga ttggtttccg ctggggagaa aaaggcaaat ggaatctgga
     2521 atccattgcc gccggtaagg aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc
     2581 tgttgcaggc gtggccttcc cctactttgg cggcattgaa aatccgcatt ttcacagcgt
     2641 aaaacacaat ccggtgctgg tgcgccaatt gcccgttaaa aacctgacgt tagtcgatgg
     2701 caacacctgt ccggtggtca gcgtttatga tttggtgctg gcgaattacg gcctcgatcg
     2761 cgggctggaa gatgaaaaca gcgcgaaaga ttacgctgaa atcaaaccgt acaccccagc
     2821 ctggggtgag caaattaccg gcgtaccgcg ccagtatatt gaaaccatcg cccgcgaatt
     2881 tgccgatacc gcccataaaa cgcatggacg ctcaatgatt atcctcggcg ctggcgtcaa
     2941 ccactggtat cacatggata tgaactaccg tgggatgatc aatatgctga tcttctgcgg
     3001 ttgtgtcggg caaagcggcg gcggctgggc gcactatgtc ggtcaggaaa aactgcgccc
     3061 acaaaccggc tggttgccgc tggcctttgc gctcgactgg aaccgcccac cgcgccagat
     3121 gaacagtacc tcgtttttct acaatcattc cagccaatgg cgctatgaaa aagtctctgc
     3181 tcaggagtta ctttcaccgc ttgccgatgc cagtaagtac agcggtcatc tgattgattt
     3241 caacgttcgc gccgaacgca tgggctggct accttctgcg ccgcagctgg ggcgtaaccc
     3301 gctcgggatt aaagctgaag ccgacaaggc cggattatcc cccacagaat ttaccgccca
     3361 ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa ccagatagcg gcagcaatca
     3421 tccgcgtaat ttgtttgtct ggcgctctaa cctgcttggc tcctccggca aaggccacga
     3481 gtatatgcag aagtatctgc tggggaccga aagcgggatt cagggcgagg aactcggtgc
     3541 cagcgacggg atcaaaccgg aagaagtcga gtggcaaact gcggcgattg aaggcaagct
     3601 cgacctgctg gtgacgctcg acttccgcat gtccagtacc tgcctgttct ccgatatcgt
     3661 tctgcccacc gccacctggt acgaaaaaga cgatatgaac acctcggata tgcatccgtt
     3721 tattcatccg ctttctgcgg cggtcgatcc ggcgtgggaa tcacgcagcg actgggaaat
     3781 ctacaaaggt atcgccaaag cattttcgca agtgtgcgtg ggtcatcttg gcaaagaaac
     3841 cgacgtggta ttacaacccc tactgcatga ctctccggca gagctctcac agccgtgtga
     3901 agtgctcgac tggcgcaaag gcgaatgcga tctgattcca ggcaaaaccg cgccgaatat
     3961 tgtggcggtg gagcgcgact accctgctac gtatgaacgc tttacctcgc tcgggccatt
     4021 gatggacaaa cttggcaatg gtggtaaagg gatttcgtgg aatacccagg atgaaatcga
     4081 tttcctcggc aaactcaatt acaccaagcg tgatggcccg gcacaggggc gtccgctgat
     4141 tgacaccgcc attgacgctt cagaagtgat tctggcactg gcaccggaaa ccaacggtca
     4201 tgttgcagtc aaagcgtggc aggcgctggg cgagatcacc gggcgcgaac atacccatct
     4261 ggcgctgcac aaagaggacg agaagattcg ctttcgcgat attcaggcgc agccgcgtaa
     4321 aattatctcc agccccacat ggtccggtct ggaaagcgat cacgtctcct ataatgcggg
     4381 atacaccaac gttcatgagt taattccgtg gcgcacgctg tcgggacgcc agcagctcta
     4441 tcaggatcat ccgtggatgc gtgcttttgg tgaaagcctg gtggcttatc gcccgcctat
     4501 cgacacccgt agcgtcagtg agatgcgcca gatcccgcca aacggcttcc cggaaaaagc
     4561 acttaacttc ctgacgccgc accagaaatg gggcattcac tcaacctaca gtgaaaacct
     4621 gctaatgctg acgctctctc gcggtggacc gattgtctgg atcagcgaaa ccgatgcccg
     4681 agaactgacc attgtcgata acgactgggt ggaagtcttt aacgccaacg gcgcgctgac
     4741 ggctcgtgcg gtggtcagcc aacgtgtacc gccgggtatg accatgatgt atcacgccca
     4801 ggaacgcatt atgaatattc ctggttcgga agtaactggc atgcgcggcg gcattcataa
     4861 ctcggtcacc cgtgtttgcc cgaaaccgac gcatatgatt ggcggttacg cgcagctggc
     4921 ctggggcttt aactactacg gcaccgtcgg ctcgaaccgc gacgaattca tcatgatccg
     4981 caagatgaag aacgttaact ggctggatga tgaaggtcgc gatcaggtac aggaggcgaa
     5041 aaaatgaaaa tacgttcgca agtcggcatg gtgcttaacc tcgacaaatg tatcggttgc
     5101 catacctgtt cggtgacctg taaaaacgtc tggaccggac gcgaaggcat ggagtacgca
     5161 tggtttaaca acgtcgaaac caaaccgggc attggttatc cgaaaaactg ggaagatcag
     5221 gaagagtggc aaggcggctg ggtccgcgat gtgaatggca agatacgccc gcgtctgggc
     5281 agcaagatgg gcgtaataac caaaatcttc gccaacccgg tggtgccgca gattgatgat
     5341 tactacgaac ctttcacctt cgactacgaa catttgcata gcgcaccgga aggcaaacat
     5401 atccctactg ctcgcccgcg ttcgctgatt gacggtaagc ggatggacaa agtgatctgg
     5461 gggccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     5521 ttcgaggcca tgcaaaagga gatgtacggg cagtttaaaa acaccttcat gatgtacctg
     5581 ccgcgcctgt gcgaacactg cctcaatccc agttgcgtgg cgacctgccc aagcggcgct
     5641 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     5701 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     5761 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     5821 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatt
     5881 gaggaagcgg cgagcaccga gcgcgaagtt gacctctatg aacgtcagtg cgaagtgttc
     5941 ctcgatccac acgatccctc agtgatcgag gaagccctga aacaaggtat tccacaaaac
     6001 gtgattgaag ctgcccagcg ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg
     6061 ctaccgctgc accctgaata tcgcaccctg ccaatggtct ggtacgttcc tccgctgtcg
     6121 ccgattcagt cctacgcaga tgcgggcggt ttgccgaaaa gcgaaggcgt gctgcccgcc
     6181 atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata tgttgagtgc cggcgatacc
     6241 ggtccggtac tgcgggcgct gaaacggatg atggcgatgc gccactatat gcgttcacaa
     6301 accgtggaag gcgttactga tactcgtgcc atcgacgaag taggcctgag cgtcgcccag
     6361 gtcgaagaaa tgtatcgcta tctcgccatt gccaactatg aagatcgttt tgttatcccg
     6421 accagccatc gggaaatggc gggcgatgcc ttcgcagaac gcaacggctg cggttttacc
     6481 tttggcgacg gttgccacgg ttcggacagt aaattcaacc tgttcaacag tagccgtatc
     6541 gatgccatca acatcaccga agtgcgcgac aaagcggagg gcgaataatg cagatcctca
     6601 aagtgatcgg cctgttgatg gagtatccgg atgagctgtt gtgggaatgc aaggaggacg
     6661 cgctggcgtt gatccgccgc gacgcgccga tgcttacgga tttcacccac aacctgctta
     6721 acgcgccgct gctggataaa caggccgaat ggtgcgaagt gtttgaccgc gggcgcacca
     6781 cgtcgctgct gctgttcgaa catgttcatg ccgagtcccg cgatcgcggc caggcaatgg
     6841 tggacctgct ggcggagtat gaaaaggtcg gcctgcagct ggattgtcgg gaactgcccg
     6901 attatctacc gctgtatctg gagtatttaa gcgtactgcc tgacgatcag gcgaaagaag
     6961 gattgctcaa tgttgcgccg atcctcgccc tgcttggcgg tcgcttaaaa caacgcgagg
     7021 caccgtggta cgcgttgttt gatgctctgc tgcaactggc gggaagtata ctttcaagtg
     7081 acagtgtcac aaaacaagta aacagcgaag agcgcgatga cacccgccag gcgcttgatg
     7141 cagtgtggga agaggaacag gtgaagttta ttgaagataa cgccacggcg tgtgacagct
     7201 cgccgcttaa tcaatatcag cgacgcttta gccaggatgt cgcgccgcag tatgtcgaca
     7261 tcagtgcggg aggtgggaaa tgattcagta tctgaacgtc tttttttacg atatctaccc
     7321 gtacatttgt gcgacggtgt ttttcctcgg cagctggctg cgctacgact acgggcagta
     7381 cacctggcgc gcctcctcaa gtcaaatgct cgataaacgc gggatggtga tatggtcgaa
     7441 tctgttccat atcggcattt tggggatttt cttcgggcac ttgttcggca tgttaacgcc
     7501 gcactggatg tacgcgtggt ttttgccagt ggcagcgaaa caactcatgg cgatggtgct
     7561 cggcggtatt tgcggtgtgt tgacgttgat tggcggcgca ggactgctgt ggcgcagtct
     7621 gacaaatcag cgcgtgcgtg ccacttccac tacgccggat atcatcatca tgagcatttt
     7681 gctgatccag tgtctgttgg ggctaagtac cataccgttt tcagcacagt atcctgatgg
     7741 tagcgaaatg atgaagctgg tgggttgggc gcaaagcatt gtcactttcc gtggtggatc
     7801 gtcagagatg ctcaacggcg tagcgttcgt cttccgcctg catctggtgt tgggaatgac
     7861 catcttcctg ctcttcccgt tcacccgact ggtgcatgtg tggagcgcgc cgtttgagta
     7921 ctttactcgt cgatatcaaa ttgtgcgttc gcggcgataa tttcaccctg gccggatagc
     7981 ccttcgctct ccggccactt gcaggctatg atagttctcc agtcacagga gaacgttaat
     8041 gaaaccgcag gtgtaccacg tcgatgcttt tacctctcaa ccatttcgcg gcaattctgc
     8101 cggtgttgtt ttccccgccg ataatcttag cgaagcacaa atgcagctta tcgcccgcga
     8161 gttaggccat tcggaaactg cttttctgct gcacagcgac gacagcgatg tgcgcatccg
     8221 ctactttacg ccaacggttg aagtgccgat ttgcggtcac gcgacagtag ctgcgcacta
     8281 tgtacgtgcg aaggtgttag gtttaggaaa ttgcacgatc tggcaaacat cgctggcagg
     8341 aaaacatcgc gtgactatcg aaaagcacaa cgatgattat cgtatttcgc tggaacaagg
     8401 tacgccgggc tttgaactgc cgctggaggg tgaagtacgc gcggcgatta tcaacgcatt
     8461 gcatcttact gaagacgata ttctgccggg tttgccgatc caggtcgcga cgaccgggca
     8521 ttctaaagtg atgatcccat tgaagccgga agtggatatc gacgctcttt cgcctgacct
     8581 taatgcgctg accgctatca gtaaacagat tggctgcaat ggtttcttcc cgttccagat
     8641 ccgtccaggc aaaaacgaaa ccgatggtcg tatgttctcg cctgcgattg gtattgtgga
     8701 agatccggtg actggcaatg ccaatggccc gatgggtgca tggttggtac atcacaacgt
     8761 attgccccac gatggcaacg tgttgtgtgt taaaggccat caggggcgcg cattggggcg
     8821 tgacggcatg attgaagtga cggtgacaat tcgtgataac caaccggaaa aagtcaccat
     8881 ttcaggcaca gcggtgattt tgtttcatgc cgaatgggca attgaaatct gaaatcgttg
     8941 cctgatgcgc ttcgcctgtc aggtctacaa ccgggctaat gtatgccgga taaggcgttt
     9001 acgccgcatc cggcaacctg acataaatta ttttcccgcc tccggatgcg tatcaaacgc
     9061 cgccatcacc agcgctaact catccacagt aaagccatgt ttcgcatcat ccacgcccag
     9121 accaaactgt tcctgcatca cggcatataa cgacgccaca tccggtagat ttcgctgctc
     9181 caccgcgtgc ccattttcat aatgggtaaa atgaaaattg gtcagtgtca gcttgccgcc
     9241 gtccggcaaa tggcggcaca tcagcaaatg atggcgaaaa tgcgactgcg gccagtgcgc
     9301 cgaccagaaa ttgcccatca cataatcgct ttgttgctgc tcgcagagat cgaagcggta
     9361 catcggctgc caatgctgat gatgattaaa ctgcaacacc caatcatcac cctcctgcaa
     9421 caaccgatac tctccgtgtg gcgtggtctg cacgagatcg gaaactaaac gaatcggcgc
     9481 ggttagcgtc tgcccaccga aaccgacatc agcaatccat ttttcccctt ccagttccac
     9541 caacagcaaa cgatgggtgc gcggcggtaa tgctggcgga tttgataaca ctacgcgccc
     9601 taacaagctg cgaacgttaa accccagctc gcgtaacacc cgctcaaaca cgccattctg
     9661 ctcaaaacag taaccgccac gacgggctat caccagtttc tcttccagcg attgatcatc
     9721 aagctgtatt tccctcggca gcaaaacgtc aaggttttca aacggaatgg tgcaattgtg
     9781 tttcaggtgc aatgcacgaa gcgtatcaat attgaccgca gcagctcccg accagttaat
     9841 acgggcaaaa tagtgattca gaatgggcgt catcaggcgt ttcccttagt ggttttctca
     9901 acttataacc tggaatagac gccgcaccgg ctattttgtg cgatcgcgga catgcatcac
     9961 gtttgcaaat cgccaatccc gttaatttcg ttacttattg cgttacggac tacgcttaaa
    10021 aaaaccttct tcgcgaggcg ataccatgag ccgattcatc cccatagaat tacaccatgc
    10081 cagccgtctg ttaaatcatg gcccaactgt cttgatcacc agtttcgatg agcaatccca
    10141 gcggcgtaac attatggctg cagcctggtc aatgccggtg gagtttgaac ctccacgcgt
    10201 ggcaattgtg gtagataaat cgacatggac cagggagttg attgaacgta acggtaagtt
    10261 tggcatcgtt atcccgggcg ttgcagcaac taactggacg tgggcggtgg gaagtgtgtc
    10321 ggtgcgtgaa gaagataaat ttaattgcta tggcattccg gtggtgaaag gtccggtgct
    10381 tggtttgcca cttgttgaag agaaatgcct ggcgtggatg gagtgccgcc tgctacctgc
    10441 gacttctgcg caacaaaaat acgacacgct gtttggcgag gtggtatcag cagcggcaga
    10501 cgcacgggta tttgtcgaag gccgctggca gtttgatgat gataagctca atacgttgca
    10561 tcatttaggt gctggcacgt ttgttactag cggcaagcgt gttacggcag gttaagcatt
    10621 acatgctgta accgggtttc tttatcaaag cctccatttg gggctcgatt tcggcatccc
    10681 agatagcttg ccaagattct ggttgaatct gctgtagagc aatgcttatc gagctatctt
    10741 tactgttcag atggcgaata ataacgtcgg taatatctgc agcaagtgct gctttttgtt
    10801 gttcgtccag ttcacgcgga aagcatttaa tgtcgatgtg cggcatgatt acgctccttt
    10861 tgaaagatgt cagtaaaatt aacacacatt gtgaatgtca tgtgtccggt aaagcgttta
    10921 tacgacaata atgcccaggg caagattacg aaagcccgct ccccgtaagg actgacgcca
    10981 ggtagtttct gcccatggct gcttttcgca tcttacgtct taaccctgcc ttgaatacct
    11041 tatcattcgt caaaatatta atagcaatgt gccgtatccc tgaaaataat tctgctgcat
    11101 ttcctcttct tattttgcag tcgtcttcat tcattaccac gt
//
LOCUS       DAJTYZ010000130        10919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000130 DAJTYZ010000000
VERSION     DAJTYZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.130"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..302)
                     /locus_tag="OO596_003317"
     CDS             complement(<1..302)
                     /locus_tag="OO596_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR6719099.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA"
     gene            complement(321..2198)
                     /gene="bglF"
                     /locus_tag="OO596_003318"
     CDS             complement(321..2198)
                     /gene="bglF"
                     /locus_tag="OO596_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6719100.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2332..3168)
                     /gene="bglG"
                     /locus_tag="OO596_003319"
     CDS             complement(2332..3168)
                     /gene="bglG"
                     /locus_tag="OO596_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6719101.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3454..4179)
                     /gene="phoU"
                     /locus_tag="OO596_003320"
     CDS             complement(3454..4179)
                     /gene="phoU"
                     /locus_tag="OO596_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6719102.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4194..4967)
                     /gene="pstB"
                     /locus_tag="OO596_003321"
     CDS             complement(4194..4967)
                     /gene="pstB"
                     /locus_tag="OO596_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6719103.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5058..5948)
                     /gene="pstA"
                     /locus_tag="OO596_003322"
     CDS             complement(5058..5948)
                     /gene="pstA"
                     /locus_tag="OO596_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6719104.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5948..6907)
                     /gene="pstC"
                     /locus_tag="OO596_003323"
     CDS             complement(5948..6907)
                     /gene="pstC"
                     /locus_tag="OO596_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6719105.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(6994..8034)
                     /gene="pstS"
                     /locus_tag="OO596_003324"
     CDS             complement(6994..8034)
                     /gene="pstS"
                     /locus_tag="OO596_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6719106.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8282..9355)
                     /locus_tag="OO596_003325"
     CDS             complement(8282..9355)
                     /locus_tag="OO596_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6719107.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9366..>10919)
                     /locus_tag="OO596_003326"
     CDS             complement(9366..>10919)
                     /locus_tag="OO596_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6719108.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2850 a         2824 c         2477 g         2768 t
ORIGIN      
        1 gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc aatggaaata
       61 cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta acggtgataa
      121 aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc catttttccc
      181 ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc cagccaggca
      241 ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg aaatgctttc
      301 ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc tgcgcttatc
      361 tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat taataccggc
      421 gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat cagccgatcg
      481 cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc gtccagtttt
      541 acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc aatgccaatg
      601 gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg cacttcacca
      661 accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt ggtatcagcg
      721 acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc ctgctcaggt
      781 ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg acgtttagcg
      841 gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat gacaccgcca
      901 ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg catgaaggtg
      961 aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat gatggtggcc
     1021 cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag attaacgcca
     1081 tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc cgccaccact
     1141 tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac ctgcgccata
     1201 atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa gttattgata
     1261 cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca gaagccgccc
     1321 attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc ggcggcaatc
     1381 agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg tgtgataacc
     1441 atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca cgcattaagt
     1501 cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac cgatgacgag
     1561 taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc cgctttttgc
     1621 ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat caccatggca
     1681 gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat cgggaagaac
     1741 caaaacaagg catcactggc gctgaataaa attaaataag taccactttg ttcggtcgtc
     1801 cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc cgccatcaaa
     1861 ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag cagattacct
     1921 ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac gctgttgacc
     1981 gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc accgctttcc
     2041 accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt gctttcatcc
     2101 tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt atctgcgccc
     2161 ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct accgctttgc
     2221 ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag caattttttt
     2281 caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat ttcagtgttc
     2341 tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg aaattttacg
     2401 ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg cttgcgggta
     2461 attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag catgttcaag
     2521 aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca agctttcttc
     2581 ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca ttaactgcgt
     2641 gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat gcatggcaat
     2701 aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa tggttaacgc
     2761 ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca gcaacgggtt
     2821 gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt cagttagcga
     2881 gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga taatacgatc
     2941 acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc gcccgttcag
     3001 ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc tttcgccagc
     3061 gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt gatcatcaat
     3121 aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag caaggacctt
     3181 ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag gttttgcctg
     3241 cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat ccaggttcgg
     3301 aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa atatgaccat
     3361 gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata taacaaatcc
     3421 caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt ttcccgccag
     3481 cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca cgtagtagaa
     3541 gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac gcgcgcagaa
     3601 cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg tcatcagttg
     3661 acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa tacgtaccgc
     3721 ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct ggatggtatg
     3781 acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga atttctccag
     3841 cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg caatggtttt
     3901 actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg cgataatgcg
     3961 cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc cttcgatcac
     4021 gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag aaagctgctg
     4081 ctccaccatg ccacccatgg tcatcacctg cgtgcggata ctttccagtt cggcgttgaa
     4141 ctggccggaa atatgtttat taagattgag actgtccata acgcactcct gaattaaccg
     4201 taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa cagatcgtcc
     4261 gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc ggaacaacgc
     4321 gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt cagttcggtg
     4381 atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg ttcgtcgagc
     4441 agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg ttgctgacca
     4501 ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca caatgcggct
     4561 ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt ctcaaacaga
     4621 cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg tttctggaac
     4681 accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag gatgttgtcg
     4741 ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa cattttgttg
     4801 aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc cgttacctgg
     4861 tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta gtagaagttc
     4921 aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc aatcttttcc
     4981 tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa ggcgttcacg
     5041 ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg cgccagaatg
     5101 ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag ttgttgccat
     5161 tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc gatcggctgc
     5221 atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg cgcggtttca
     5281 ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac cgacgctttc
     5341 agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc cgcttcacgc
     5401 aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat cggcacctgc
     5461 aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg tgccaccacg
     5521 atggtgtaga caaacagacc aaccacaatc gacggcacgg agagcagaat gtcgttaatg
     5581 aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata aatccccgcc
     5641 ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc gctacccgcc
     5701 agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc agtgaacagc
     5761 gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca gatcagccag
     5821 aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt tttgaggcgg
     5881 cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg catttcaacc
     5941 atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac ttcgatgcgg
     6001 cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt gcggcaacgt
     6061 gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata ctattgcccg
     6121 gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag gtcaccgcca
     6181 tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca ccatttttgg
     6241 tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata ccgtaggccg
     6301 actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc gcaatgtacg
     6361 gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca aatgcggggc
     6421 cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc ggctcctgaa
     6481 agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa acgatacttg
     6541 gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag ccaggcgcaa
     6601 gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc agcgcgataa
     6661 acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg ttcggtgcat
     6721 cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc caggaggaga
     6781 tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc gccagtttta
     6841 ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca ggcttggttg
     6901 cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa tgccggatgc
     6961 ggcgtaaaac accgtacccg gcctggagtt ttattagtac agcggcttac cgctactgtc
     7021 tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg gcaggctggc
     7081 gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga agaatttcag
     7141 cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga acgtggtaga
     7201 ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga aggttttgct
     7261 ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa ccggtttacc
     7321 atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag catattcaac
     7381 ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt tacctttacc
     7441 gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt tccactcttc
     7501 gttcactttc gccaggtagc tggtgaagac gaaggtagtc ccggagccat ctgcgcggcg
     7561 tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg cgatggtttc
     7621 atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac catccagcac
     7681 cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc caatcacggt
     7741 cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg agtcagaggc
     7801 accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac cgataccctg
     7861 gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg catacaccgg
     7921 cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag agaaagcgct
     7981 catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt tcataatgtc
     8041 tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata ggacaaacag
     8101 gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta acaaataact
     8161 tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca tgatgcattt
     8221 aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt tgtcagacct
     8281 attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc ggggctgggc
     8341 gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat ttttcaccat
     8401 acactgttga tttacccatg tctgttggca actcaccacc gttcactttg atttccttat
     8461 tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc attgaattgt
     8521 ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc agtatcccca
     8581 ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc gcacttccaa
     8641 caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact ggctgtaaat
     8701 tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca tcagccatcg
     8761 gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc actatctttg
     8821 tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat actatcgctt
     8881 tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt gtattaccag
     8941 acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca agaatatcta
     9001 tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta gagtgaaccc
     9061 cagcataagt taatgcctgg tttatttttg acgtataata aatatagggg aattctccta
     9121 cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt acagtggtat
     9181 taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag aaatcaagcg
     9241 tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca agtgctacac
     9301 tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg ttcataaaaa
     9361 tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc tggtgtcgaa
     9421 agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa cgtaacctgt
     9481 cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat tcctgaatct
     9541 gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt accatttgca
     9601 tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc tttaaccact
     9661 gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt cgacttcgag
     9721 aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg tgtcttgacc
     9781 gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa cccggcggcc
     9841 ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt taccccttcg
     9901 ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt ttggctgtag
     9961 ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat attaccgcca
    10021 acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt atttctttcc
    10081 agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt ttgcgaaaca
    10141 gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa agagagcatg
    10201 tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg tctggcagta
    10261 ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct gtagccaaat
    10321 tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa tgtcaggtat
    10381 aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca actacgtcgg
    10441 ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc attgagggta
    10501 tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc tgtgtcgtca
    10561 aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc atggctaaaa
    10621 ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag atctttaccg
    10681 acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata taaagtgatc
    10741 ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa tccgtcttca
    10801 tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt aagaatcgct
    10861 aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt gatggttac
//
LOCUS       DAJTYZ010000131        10829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000131 DAJTYZ010000000
VERSION     DAJTYZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.131"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OO596_003327"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OO596_003327"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6719109.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OO596_003328"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OO596_003328"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6719110.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3388)
                     /locus_tag="OO596_003329"
     CDS             complement(2696..3388)
                     /locus_tag="OO596_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR6719111.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3518..5278
                     /locus_tag="OO596_003330"
     CDS             3518..5278
                     /locus_tag="OO596_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6719112.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5684..6541
                     /gene="focA"
                     /locus_tag="OO596_003331"
     CDS             5684..6541
                     /gene="focA"
                     /locus_tag="OO596_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6719113.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6596..8878
                     /gene="pflB"
                     /locus_tag="OO596_003332"
     CDS             6596..8878
                     /gene="pflB"
                     /locus_tag="OO596_003332"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6719114.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9070..9810
                     /gene="pflA"
                     /locus_tag="OO596_003333"
     CDS             9070..9810
                     /gene="pflA"
                     /locus_tag="OO596_003333"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6719115.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9903..10079)
                     /locus_tag="OO596_003334"
     CDS             complement(9903..10079)
                     /locus_tag="OO596_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR6719116.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            10179..>10829
                     /locus_tag="OO596_003335"
     CDS             10179..>10829
                     /locus_tag="OO596_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6719117.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGN"
BASE COUNT         2788 a         2709 c         2566 g         2766 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtctctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgcccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaggat ggaataatcc agaatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccattgc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgtggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gtttttcccc cgctttcata tgaatgattt cactacaacg aatacaggca tagtccgccg
     2821 actccggcac cactttgtaa cgctgcgtac aatcactggg taaaataagt aaacccggtt
     2881 tcaccttgtc atcttcaaag aaatagacac tgatttgccc gttggtttcg agaatcgcca
     2941 gacgtacctg ccccagctgc tccacgccac gcaatcgtag ctccataaag aactcaaatt
     3001 ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca ataataacaa
     3061 ccggcttacc ttccagtaga tcttccagtt tttcactgtg cgccatcaac cacattacca
     3121 ggcggtataa caacgccaga gtgataaaga cgataagtac cggcaccatc ggcacatcat
     3181 cataaaacgc cacatctccc gccgctgaac ccagcgtgag aatgattaac acttcaaaca
     3241 gcgacatctg tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa accagtacaa
     3301 aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact ttatcaaatg
     3361 ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt atccgctaaa
     3421 agcatagtca acagttcatc ggcatggcgg cggtgatagc gcctttgaga taaaacccgt
     3481 attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat ttatccccgg
     3541 caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt cagacctcgg
     3601 ctttcagatt gaagaggcct cctggctgaa tcctgtgcct aacgtctggt ctgtacatat
     3661 tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca aaaaagcggc
     3721 gctggcttct gcacttggtg aatatttcga gcgtctctca accaactact tttttgctga
     3781 tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg cattatccca acgaaaaatg
     3841 gttcccactg accgaaaatg acgatgtgcc agaaggacta ctcgatgacc gtctgcgcgc
     3901 gttttatgat ccggagaatg aactgaccgg cagcatgctg attgacctac aatccggtaa
     3961 cgaagatcgt ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc agaccgttta
     4021 tattccgatg aatatcattg gtaacctgta cgtctccaac ggtatgtccg caggtaatac
     4081 ccgcaacgaa gcacgcgttc agggattgtc tgaagttttc gaacgctacg tgaaaaaccg
     4141 cattattgct gaaagcatca gcctgccaga gatcccggca gacgtgctgg cgcgttaccc
     4201 agcagtagtt gaagccatcg aaacgctgga agcggagggt ttcccaatct tcgcttatga
     4261 cggttcgctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc ctgctaacgg
     4321 tacctgcttt gcctctttcg gtgcgcatcc tgattttggc gtagcactgg aacgtaccgt
     4381 gaccgagctg ctgcaaggtc gtggcctgaa agatttggat gtgtttactc cgccaacctt
     4441 cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg attccagcgg
     4501 tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgtgg actggaattt
     4561 ctccggcacc acggaagaag agtttgctac gctgatggct atcttcaaca aagaagataa
     4621 agaagtttat attgccgatt acgagcatct gggcgtttat gcttgccgta ttatcgtgcc
     4681 tggcatgtcc gatatttatc cggctgaaga tctgtggctc gcgaataaca gtatgggcag
     4741 ccatttacgt gaaacgattc tttcgctacc aggcagcgag tgggaaaaag aagattacct
     4801 gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg tgcgtgagct
     4861 gttgggtctg gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg gtgaattaaa
     4921 agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga ccgaatggac
     4981 gatggagttt aactcatcag tatttagtcc ggaacgcgcc aactattatc gctgcctgca
     5041 aacgttgtta ttactggcac aggaagaaga tcgccagccg ctgcaatatc tgaatgcgtt
     5101 tgttcgcatg tacggcgcag atgccgtaga agccgccagt gcggcaatga gcggcgaagc
     5161 ggcgttttac ggcctgcaac cggtagatag cgatctgcac gcgtttgctg cacatcagtc
     5221 gttgttgaag gcctacgaaa agctgcagcg cgccaaagca gcattctggg caaaataaaa
     5281 tcaaatagcc tacgcaatgt aggcttaatg attagtctga gttatattac ggggcgtttt
     5341 tttaatgccc cgctttacat atatttgcat taataaaata attgtaatta taaggttaaa
     5401 tatcggtaat ttgtatttaa taaatacgat cgatattgtt actttattcg cctgatgctc
     5461 ccttttaatt aactgtttta gcggaggatg cggaaaaaat tcaactcatt tgttaatttt
     5521 taaaatttat ttttatttgg ataatcaaat gtttactccg tatctgcata aaaaccatgc
     5581 gagttacggg cctataagcc aggcgagata tgatctatat caatttctca tctataatgc
     5641 tttgttagta tctcgtcgcc gacttaataa agagagagtt agtgtgaaag ctgacaaccc
     5701 ttttgatctt ttacttcctg ctgcaatggc caaagtggcc gaagaggcgg gtgtctataa
     5761 agcaacgaaa catccgctta agactttcta tctggcgatt accgccggtg ttttcatctc
     5821 aatcgcattc gtcttctata tcacagcaac cactggcaca ggcacaatgc ccttcggcat
     5881 ggcaaaactg gttggcggca tttgcttctc tctggggctg attctttgtg ttgtctgcgg
     5941 agccgatctc tttacttcca ccgtgttgat tgttgttgct aaggcgagtg ggcgcatcac
     6001 ctggggtcag ttggcgaaaa actggctaaa tgtctatttt ggcaacctgg tcggcgcact
     6061 gctgtttgta cttttaatgt ggctttccgg cgagtatatg accgcaaatg gtcaatgggg
     6121 actaaacgtc ctacaaaccg ccgaccacaa agtgcaccat acttttattg aggccgtctg
     6181 tcttggtatc ctggcaaacc tgatggtatg tctggcagta tggatgagtt attctggccg
     6241 cagcctgatg gacaaagcgt tcattatggt gctgccggtc gcgatgtttg ttgccagcgg
     6301 ttttgagcac agtatcgcaa acatgtttat gatcccgatg ggtattgtaa tccgcgactt
     6361 cgcatccccg gaattttgga ccgcagtcgg ttctgcaccg gaaaattttt ctcacctgac
     6421 cgtgatgaat ttcatcactg ataacctgat tccggttacg atcggcaaca ttatcggtgg
     6481 tggtttgttg gttgggttga catactgggt catttacctg cgtgaaaacg accaccatta
     6541 atggttgtcg aagtacgcag taaataaaaa atccacttaa gaaggtaggt gttacatgtc
     6601 cgagcttaat gaaaagttag ccacagcctg ggaaggtttt accaaaggtg actggcagaa
     6661 tgaagtaaac gtccgtgact tcattcagaa aaactacact ccgtacgagg gtgacgagtc
     6721 cttcctggct ggcgctactg aagcgaccac caccctgtgg gacaaagtaa tggaaggcgt
     6781 taaactggaa aaccgcactc acgcgccagt tgactttgac accgctgttg cttccaccat
     6841 cacctctcac gacgctggct acatcaacaa gcagcttgag aaaatcgttg gtctgcagac
     6901 tgaagctccg ctgaaacgtg ctcttatccc gttcggtggt atcaaaatga tcgaaggttc
     6961 ctgcaaagcg tacaaccgcg aactggatcc gatgatcaaa aaaatcttca ctgaataccg
     7021 taaaactcac aaccagggcg tgttcgacgt ttacactccg gacatcctgc gttgccgtaa
     7081 atctggtgtt ctgaccggtc tgccagatgc atatggccgt ggccgtatca tcggtgacta
     7141 ccgtcgcgtt gcgctgtacg gtatcgacta cctgatgaaa gacaaactgg cacagttcac
     7201 ttctctgcag gctgatctgg aaaacggcgt aaacttggaa cagactatcc gtctgcgcga
     7261 agaaatcgct gaacagcacc gcgctctggg tcagatgaaa gaaatggctg cgaaatacgg
     7321 ctacgacatc tctggtccgg ctaccaacgc tcaggaagct atccagtgga cttacttcgg
     7381 ctacctggct gctgttaagt ctcagaacgg tgctgcaatg tccttcggtc gtacctccac
     7441 cttcctggat gtgtacatcg aacgtgacct gaaagctggc aagatcaccg aacaagaagc
     7501 gcaggaaatg gttgaccacc tggtcatgaa actgcgtatg gttcgcttcc tgcgtactcc
     7561 ggaatacgat gaactgttct ctggcgaccc gatctgggca accgaatcta tcggtggtat
     7621 gggcctcgac ggtcgtaccc tggttaccaa aaacagcttc cgtttcctga acaccctgta
     7681 caccatgggt ccgtctccgg aaccgaacat gaccattctg tggtctgaaa aactgccgct
     7741 gaacttcaag aaattcgccg ctaaagtgtc catcgacacc tcttctctgc agtatgagaa
     7801 cgatgacctg atgcgtccgg acttcaacaa cgatgactac gctattgctt gctgcgtaag
     7861 cccgatgatc gttggtaaac aaatgcagtt cttcggtgcg cgtgcaaacc tggcgaaaac
     7921 catgctgtac gcaatcaacg gcggcgttga cgaaaaactg aaaatgcagg ttggtccgaa
     7981 gtctgaaccg atcaaaggcg atgtcctgaa ctatgatgaa gtgatggagc gcatggatca
     8041 cttcatggac tggctggcta aacagtacat cactgcacta aacatcatcc actacatgca
     8101 cgacaagtac agctacgaag cctctctgat ggcgctgcac gaccgtgacg ttatccgcac
     8161 catggcgtgt ggtatcgctg gtctgtccgt tgctgctgac tccctgtctg caatcaaata
     8221 tgcgaaagtt aaaccgattc gtgacgaaga cggtctggct atcgacttcg aaatcgaagg
     8281 cgaatacccg cagtttggta acaacgatcc gcgtgtagat gacctggctg ttgacctggt
     8341 agaacgtttc atgaagaaaa ttcagaaact gcacacctac cgtgacgcta tcccgactca
     8401 gtctgttctg accatcactt ctaacgttgt gtatggtaag aaaacgggta acaccccaga
     8461 cggtcgtcgt gctggcgcgc cgttcggacc gggtgctaac ccgatgcacg gtcgtgacca
     8521 gaaaggtgca gtagcctctc tgacttccgt tgctaaactg ccgtttgctt acgctaaaga
     8581 tggtatctcc tacaccttct ctatcgttcc gaacgcactg ggtaaagacg acgaagttcg
     8641 taagaccaac ctggctggtc tgatggatgg ttacttccac cacgaagcat ccatcgaagg
     8701 tggtcagcac ctgaacgtta acgtgatgaa ccgtgaaatg ctgctcgacg cgatggaaaa
     8761 cccggaaaaa tatccgcagc tgaccatccg tgtatctggc tacgcagtac gtttcaactc
     8821 gctgactaaa gaacagcagc aggacgttat tactcgtacc ttcactcaat ctatgtaatt
     8881 agatttgact gaaatcgtac agtaaaaagc gtacaataaa ggctccacga aagtggggcc
     8941 ttttttagca cgagagcctt ttttgtcagc tatctatact ctaaggtgac tgccaaaaca
     9001 gactcgacgt agccttcgag ctgcgcacca acacggcctc agatgggcca catctggaga
     9061 aacaccgcaa tgtcagttat tggtcgcatt cactcctttg aatcctgtgg aaccgtagac
     9121 ggcccgggta ttcgctttat cacctttttc cagggctgcc tgatgcgctg cctgtattgt
     9181 cataaccgcg acacctggga tacgcatggc ggtaaagaag ttaccgttga agatttaatg
     9241 aaagaggtgg tgacctatcg ccactttatg aacgcttccg gcggcggcgt taccgcatcc
     9301 ggcggtgagg caatcctaca agctgagttt gttcgtgact ggttccgcgc ctgcaaaaaa
     9361 gaaggcattc atacctgtct ggacaccaac ggttttgttc gtcgttacga tccggtgatt
     9421 gatgaactgc tggaagtaac cgacctggta atgctcgatc tcaaacagat gaacgacgag
     9481 atccaccaaa atctggttgg agtttccaat caccgcacgc tggagttcgc caaatatctg
     9541 gcgaacaaaa atgtgaaggt gtggatccgc tacgttgttg tcccaggctg gtctgacgat
     9601 gacgattcag cgcatcgcct cggtgaattt acccgtgata tgggcaacgt tgagaaaatc
     9661 gagcttctcc cctaccacga gctgggcaaa cacaaatggg tggcaatggg tgaagagtac
     9721 aaactcgacg gtgttaaacc accgaagaaa gagaccatgg aacgcgtgaa aggcattctt
     9781 gagcagtacg gtcataaggt aatgttctaa tttccccgta aagcggcaac tttattgagt
     9841 tgccgctttt ttattccgca cttcattctg ctacagcgtt tttaacacat cttatagtgc
     9901 atctaccata tcccgtacct gagataataa cgactgataa tattacgccg atataaatcg
     9961 agttccgtca cctgtatttt ccgctccatt gcccgctgat ggatcgcatc agcaatatgt
    10021 gcggtcaatg aatcatgacg aggatgggcc cataccaaat aaatacgttc agactgcata
    10081 attttacctc cacgttaagt tctggagcgc actttacgca ggcggcataa taatgtaaac
    10141 gtgatgattc aggatgagtt aatgaaggat tttcactaat gcggatgaat atgtctgact
    10201 ttgccacttt ctttgctgtg gcccgtaatc aaagctttcg tgcagcgggc gatgagttag
    10261 gcttatcctc gtccgccatt agccatagta ttaaaacact ggaacaacgt cttaaaattc
    10321 gcttattcaa tcggacaacc cgaagcgtat cgttaacgga agcgggttct aatctctatg
    10381 aacgattgcg cccagctttc gacgaaattc agatcatgtt agatgagatg aatgattttc
    10441 gtctgacacc aacaggaacg ctcaaaatca atgccgcccg ggtagctgca cgtatatttt
    10501 taatgccttt actggttggt ttcacacgtg aatatcctga tattaaagtc gagctcacaa
    10561 ctgacgactc tcttgtcgat atcgtccaac agggctttga cgctggcgtt cgacttagtg
    10621 gcatcgtcga aaaagatatg atcagcgtgg cgatcggccc accagtgaaa ttatgtgtag
    10681 cagcaacgcc agagtacttt gcaagatatg gcaaaccccg ccatcctcat gatctgctta
    10741 atcatcaatg tgttgtattt cgttatccca gcggcaaacc tttccactgg caatttgcaa
    10801 aggagcagga aattgctgtc gcagggaat
//
LOCUS       DAJTYZ010000132        10754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000132 DAJTYZ010000000
VERSION     DAJTYZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.132"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..264)
                     /locus_tag="OO596_003336"
     CDS             complement(<1..264)
                     /locus_tag="OO596_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6719118.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFE"
     gene            complement(387..2033)
                     /gene="fumB"
                     /locus_tag="OO596_003337"
     CDS             complement(387..2033)
                     /gene="fumB"
                     /locus_tag="OO596_003337"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6719119.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2111..3451)
                     /gene="dcuB"
                     /locus_tag="OO596_003338"
     CDS             complement(2111..3451)
                     /gene="dcuB"
                     /locus_tag="OO596_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6719120.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4022..4741)
                     /gene="dcuR"
                     /locus_tag="OO596_003339"
     CDS             complement(4022..4741)
                     /gene="dcuR"
                     /locus_tag="OO596_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6719121.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(4738..6369)
                     /gene="dcuS"
                     /locus_tag="OO596_003340"
     CDS             complement(4738..6369)
                     /gene="dcuS"
                     /locus_tag="OO596_003340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6719122.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6550..6780
                     /locus_tag="OO596_003341"
     CDS             6550..6780
                     /locus_tag="OO596_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCR6719123.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            6792..7064
                     /locus_tag="OO596_003342"
     CDS             6792..7064
                     /locus_tag="OO596_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6719124.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7291..7587
                     /gene="ghoS"
                     /locus_tag="OO596_003343"
     CDS             7291..7587
                     /gene="ghoS"
                     /locus_tag="OO596_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6719125.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7615..7788
                     /gene="ghoT"
                     /locus_tag="OO596_003344"
     CDS             7615..7788
                     /gene="ghoT"
                     /locus_tag="OO596_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCR6719126.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(7907..9424)
                     /gene="lysS"
                     /locus_tag="OO596_003345"
     CDS             complement(7907..9424)
                     /gene="lysS"
                     /locus_tag="OO596_003345"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6719127.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(9661..>10754)
                     /gene="dtpC"
                     /locus_tag="OO596_003346"
     CDS             complement(9661..>10754)
                     /gene="dtpC"
                     /locus_tag="OO596_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCR6719128.1"
                     /translation="LLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLAAQWYGW
                     HVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVMLCLAPV
                     FFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTLFWVLAQ
                     QGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESRGNSTLR
                     VWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFIDPVAIAQ
                     ITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQMGEWTL
                     ACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT         2778 a         2576 c         2636 g         2764 t
ORIGIN      
        1 ctcaaaaccg atggggactt tcgcgtaggt gtattgcccg ccgaccatca ggatgatggc
       61 gtgaaagaaa atgagcgtat agagcagcgg cgttaacgga taacgtttag cggtggcaag
      121 cagtagcggc acgacaataa tcaccggtgt cacttccatc agccaggtga gtttgtcatg
      181 ggccgaaatg ccggtataaa tcaggattaa cgttagcgcc agtgcgccgg tgttaagaat
      241 taacggtttg agtgtgcggg tcatggtgag ttcgcaagtc agggaaaata ctgactattc
      301 accagcaagc atgaaattac aatcgcaaaa gaggtggccc agggggatca cctggcagca
      361 tgctgccagg cgctgggccg aagaggttac ttagtgcagt tcgcgcactg tttgttgacg
      421 atttgctgga agaagtcgtt acctttgtca tcgaccagga taaacgccgg gaaatcttct
      481 acttcgattt tccagatagc ttccataccc agctccggat aagcgacgca ttccagatgc
      541 ttgatgctct gctgcgccag taccgccgcc ggaccgccga tgctaccgag gtagaagccg
      601 ccgtgtttat gacacgcgtc ggtaacctgc tggctgcggt tgcctttcgc cagcatgatc
      661 atgctgccgc cgtgggattg cagcagatcc acataggagt ccatacgacc tgcggtggtt
      721 gggccaagtg aacctgatgg ataaccggca ggagttttcg ccggacccgc gtagtagatc
      781 gggtgatctt tgatgtactg cggaagttct ttaccggcgt caatcagctc tttcagcttg
      841 gcgtgtgcaa tatctcgacc cacaataatg gtgccagtga gcgacaaacg agtggaaacc
      901 ggatattgcg aaagctgggc gaggatctct ttcatcgggc ggttaaggtc gaccttcacc
      961 gcttcgcctt caccggcctg acgcagttct tgtggaatgt actgacctgg attgtgttcc
     1021 agtttttcga tccagatacc ttcgcggttg attttcgctt taatgttacg gtcagcggag
     1081 caggagacgc ccatgcctac cgggcaggat gcgccgtgac gtggcagacg gataacgcga
     1141 atgtcgtgcg cgaagtattt accgccaaac tgcgcgccaa gaccgagttt ctgggcctct
     1201 tccagcagtt cctgttccag ctggacatcg cggaacgcct gaccatgttc gttcccttcc
     1261 gtcggcagtt catcgtaata gtgagcgctt gctaatttga cggttttcag gttggtttcc
     1321 gcagacgtac cgccaatcac aaacgcgata tggtacggcg ggcaggctgc agtaccgagg
     1381 gtacgcattt tctcgacgag gaagtttttc agtttgccgg gagtcagcag ggctttggtt
     1441 tcctggtaga gatacgtttt gttggcagag ccgccgcctt tcgcaacgca aaggaatttg
     1501 tactcatcgc catctaccgc gtacaggtcg atttgcgcag gcaggttagt gccggtgttg
     1561 acctctttgt acatgtccag cgccgcgttt tgcgagtagc gcaggttatc ttcgatatag
     1621 gtgttataga cgcctttcga cagcgcttct tcatcaccgc caccggtcca cacgcgctgg
     1681 ccttttttac cgacgatgat cgcggtgccg gtatcctggc aggtcggcag cacgcctttg
     1741 gcggcgattt cggagtttct taagaattgc agtgccacgt acttgtcgtt ttcgctggct
     1801 tctggatcgt gaagaatagc cgcaacctgt ttctggtgtg ccggacggag catgaaagaa
     1861 gcgtcgtgaa aggcttgctg cgccagcagg gtcagggctt ctggttccac tttcaggatg
     1921 gtttcgccgt cgaagtcggc aacgctaacg taatcggaag tgagtagata gtattcggta
     1981 ttgtctttcc ccatcgggaa aggtgcctgg tagataaagg gtttgtttga catagcttcc
     2041 agcctgtaac tctgtatttg ttattcgaaa atggcgtgcc gcgtagtcac gggcggcacg
     2101 caaagtgcat ttataagaac ccgtacatcg cggcgaagat ccagccgaag acgcacgata
     2161 cgctcacacc aatcaacccc ggcagaataa agctgtggtt gatgacgaag cgaccgatgt
     2221 gggtggtgcc ggaacggtca aactgaatcg ctgccagatc gctcggataa gtcggcagga
     2281 tgtaataacc gtagcaagcc ggtgctgaag ccacgatgta tgccggatca acgccgatcg
     2341 ccagcgcgac cggaacaatc gccgccagcg ccgcagcctg agagtttaca aacttggaaa
     2401 ccagcagcag aacaatggca taggcccacg gatactcttt caccatttca cccagtacgc
     2461 cctgaatttc agacatatgc gcaccgaaca tggtttctgc catccatgcg ataccgtaca
     2521 ccgccacgat ggcgatcata ccggaacgga agacttcgtt ttttgagata gacgcgggat
     2581 tggttttggt caggataata atcagcgccc cggtcagcag cataaacatc tgaataacca
     2641 gtaccatcga cagcggtttg ccgccgaagg atggacgcag gtccgaatca gcaccaagaa
     2701 gggcgactac agcgattgcc ccgaggaaaa tccacattgc cagccagttg cttttcggca
     2761 gttttttgtc cagcagcgtc gcggtatcac cgtaaacata ctcacggttt tccggtacgg
     2821 agatgaattt ctggaactct tcgtctttat ccagatcttt accacggaac cagctgaaga
     2881 taccgatcgc caggataccg attaacgtcg atggaatggt gattgccagc aggtcgagga
     2941 actcaagatg gcgaccatca aaggtgacat tacccagcat tgcaaccaga gacacgaccg
     3001 caaccgacac cggactggcg atgatcccca tctgtgcacc gatagaactt gccgccatcg
     3061 gacgttccgg acggatgttg ttcttaatgg cgacgtcgta gatgatcggc agaatggtgt
     3121 aaaccacatg acccgtaccg caaagaatgg tcagcgtaca ggtcacaaac ggcgcgacaa
     3181 ttgagacata tttcgggttg cggcgcagca gcttctcggc aatttgcagc atgacatcaa
     3241 gaccgcccga agcttgcaag gtcgccgatg ccgccaccac cgcaatgata accagcatga
     3301 catcaaccgg tggtttacct ggctgaaggt ggaagacgaa gaccagaatg accagaccga
     3361 taccgcctaa taaacccagc gcgataccac cctttctggc accataaaac agacatatca
     3421 gtattatgat aagttggata gtaaataaca tgtgtgaacc ctcgcgataa tcctatttaa
     3481 atttttgctg aatagatcac agtcacgttc tgttttgtat gaactgtttt cagagcctga
     3541 ctaaatatcc gtttggtcgt taccggcttt agcaaatacc tcacagtgaa tattggctga
     3601 ttaaagcagc ggcaaattta tgcactgtga taagcggctt tttcagagag aaagactctc
     3661 tggcgatggg tttatagttt gtactgcgca gatgttaatg ttttgtttgc tgttatctta
     3721 ttgatattta tcggtctatt ttttgttctc agggcagatg tcattaggtt tatagattaa
     3781 tctgatctac ccatttgtgg gtaaaaatac acataatgcg ggtgacataa tagttaatta
     3841 acttttgtta gcgttttgaa attaaaaaca ccgttcacct gaagagatat taatttttag
     3901 cgatgatgca gggataatta tatttgatct ggcacaagtt ttactgatga aggatgtaac
     3961 ttgtgccagg ggtattttgc attacggtaa taattattac ttacagataa ccagcgatcc
     4021 gttattggca atattgtttc agtagtgagt agtgttctgc ctgaatacgg taacggtaaa
     4081 ctggacgccc cgtgacgcca taatggatac tggtgaacaa gatgtggcag ttgaccagcc
     4141 agatgaggta tttacggcag gaaacacgcg aaatgttaac ctcgttggct agctcgtcgg
     4201 ttgaaaattc atagtcctga tgcgcgtcaa tccactggca cagtgtgcgt aacgtctgcg
     4261 gcgttaagcc ttttggcaag cgacgaggat cctgttcgtt ggagctgctg ccgtggatta
     4321 gctgatcaag ctcggcctgg tcataatact gatgtttttc cagcgccatt ttcttttgcc
     4381 gccagtcggt gagcgcctct tcaaagcggg aagcctggaa gggtttgatc aggtaatcca
     4441 cgacaccgta atgcagcgaa tctttaatgg ttgccgcatc ggctgcggag gagatgacaa
     4501 tcacatcact tttgcaacgc gcgttatgca ggacaggcag taaatcgagc ccgttctctt
     4561 tttgcatata gatatcgagc aatatcaggt cgataggcgt atcgctattg aagataatct
     4621 ctttggcttt ctccagcgtc gaggctgttc cacagcattg aaagcctggg atttgtgcta
     4681 cgtatcggcg attcagctcc gcgaccattg cgtcgtcatc gataattaat acattgatca
     4741 tctgttcgac ctctccccgt cccagggtat ctggacaaaa aattgtgtga aaatcccggg
     4801 ttccgattcc acggcgatgc tgccgccgag attttctacc tgttgtttga caagtgctaa
     4861 accgacgcct cgctcgcttc cttttgtcga gacacctttg tcaaaaatgt gatcgatttt
     4921 gtcgggtgcg atccccggtc catcatcgtt aacttcgcag tgcagccagc cgtgacggta
     4981 gtgcaatgtt acgctaattt cgccaccggg ttccggccct aatgcttcca gcgcgttttc
     5041 tatcagattt cccaatgtgg taatcagcgt cgcgacctgg tcctcactgc cgctgtctgg
     5101 cagctggctt tcactgttta aaatcagcgt atggcctaaa tcggtcgcgc gattaatctt
     5161 gctgattaaa aaaccagcga taaccggaga tttgatctta cccagcagag agccaatctc
     5221 ttcctgatag ttattggctg tttttagaat gtaatcttcc aactgcttat aactcttcag
     5281 atgcaataat ccgagaatca cgtgcaattt attcataaat tcgtgggatc gttcacgaag
     5341 tgcgtcggca tagttgacca gaccgtcgag tcgctgcatc agtttacgta cttcggtttt
     5401 gtccctgaag gttgaaatgg caccgatgat aacgccatta ctgcgcaccg gaacggtgtt
     5461 gatcagtaat agccggtctt taatcgtaat ctcttcgtcg cggcgcggcg taccgtcgcg
     5521 taacacttcc gagacatcta ccacctgtga ccatgagtgg ctgagcgtcg acagtttctc
     5581 atcgtcctgc gacttacggt aattcagcaa ttcttgtgcg gcatcgttga tcagcgtgac
     5641 ctcgccgcga tcgtccacgg caacgacgcc ttctttgata gactgcaaca tggcctggcg
     5701 ttgctcaaac agcgtggaga tttcgtaggg ttccaggccg aaaaggattt ttttcagtac
     5761 cttaaccaga atgcaggtgc caatcagtcc gaccagcatg ccaaataata ccgaccagat
     5821 aatgctccag cgactgtcat tgatttgttg ggtcacgcgg cttaactcaa ggccgatcgc
     5881 caccacgcca atttgtttat gattttcatc gtagatgggg gtaaatacgc gtaaagcctg
     5941 cgccagaaaa ccgcgattga tagcgacatt ttctttgcca ttcagcgctt taaggatgtc
     6001 atcaccttta aatggctgac caatacgctg ggcttcagga tgcgagtagc gaagactttg
     6061 catatcggta acgacaataa acagcagatc gttgcgtttg cgcacggctt ccgcgatggc
     6121 ctggatgccg ctctcctgcg gttttttctg caagccctga cggatttccg gcgagttggc
     6181 gagggtacgc gccactgcca gtgccttgtt ggctagcccg tctcgcgtca tatcactgat
     6241 ttgcgagaag taaatcagat gcaccaccaa tagcaccgag aacagtaccg cactgaccat
     6301 taagatcact gtggtactca atttcatcgg acgtttgcgt aacatgcggt agggcaatga
     6361 atgtctcatc agcttccttg tgtgacaaat ttcttaaaca ttatctctga tgaggtgggt
     6421 aattcaaagg gagtaagaat gattggctat ataggggaag agattctggc aacggaaact
     6481 gccagtgctg tatgaagatt ccagggctat gcttatagcg ataatcatac tgatgagaga
     6541 gggaaagtca tggatcaggc gctactggac gagggttatc gctgttatac cggcgaaaag
     6601 atcgatgtct atttcaacac tgcgatatgt cagcattctg gcaattgcgt acgtggcaac
     6661 ggcaagttat ttaatctcaa acgaaagccg tggatcatgc cggatgaagt cgacgtcgcc
     6721 actgtggtta aagtgattga tacgtgcccg agcggcgcgc tgaaataccg tcataaataa
     6781 gcgagggtaa aatggaaata cgcgaaggcc acaataaatt ttacattaat gacgaacaag
     6841 gcaagcaaat cgctgaaatt gtctttgtgc cgactggaga gaatttagcg attatcgaac
     6901 ataccgatgt cgatgaaagc ctgaaagggc aggggattgg taaacagctg gttgcaaaag
     6961 tcgtggaaaa aatgcgtcgg gaaaaacgaa aaattatccc actatgccca tttgcgaaac
     7021 acgaatttga taaaacgcgg gagtatgatg atattcgcag ttgatgggag agtacagagt
     7081 cacgatattt ttcatgctct ccgcggagtg atgcaggaga gcatctgaag ggtaggggga
     7141 tgcacaaaga atgggcagag agcgcgtttt tttgtcccaa gtcatcccct ttactgagca
     7201 aaaaaaagaa tatctcctat atgagaatca tcaatcgggg ttatcaagtt ttgcgtcccc
     7261 agagcattca atattgatag gagtcatatt atggaaggta aaaacaagtt caatacttat
     7321 gttgtttctt ttgattatcc atcatcttat tcctcagtgt tcttaaggtt aagatcattg
     7381 atgtatgata tgaatttctc ttctatcgtg gctgatgaat atgggatacc acgacaattg
     7441 aatgaaaatt ccttcgcaat aacgacatcg ttagccgcaa gtgaaatcga agatttaatc
     7501 aggcttaaat gcttagactt accggatatt gattttgacc tcaacattat gacagttgat
     7561 gactatttcc gtcagtttta caagtagcca ggaaggataa gaagaacata aaaaatggca
     7621 ctattctcta aaatattaat tttttatgtg attggtgtga acatatcctt tgtcattatc
     7681 tggtttatct cacatgagaa aacacatatt cgtttactta gtgcattcct ggtcggaata
     7741 acctggccaa tgagtctgcc tgtggcatta cttttttctc tcttttagga gctgtagttg
     7801 agtggacccg ctggttatcg tgaaacgaag cgtctctcct caaaactgga tttgcggaac
     7861 gacgaacgaa gcagtcgcct gactgcttcg ttcattaaag tgaaatttat ttctgtgggc
     7921 gcatcgccgg gaagagaata acgtcgcgaa tagtatgact gtttgtaaac agcataatca
     7981 ttcggtcgat accaataccc agaccagcgg ttggcggcag accatattcc agcgcagtta
     8041 cgtaatcttc gtcatagaac atggcttcgt cgtcacctgc agctttagcg ttaacctgtt
     8101 cctggaaacg ttcagcctga tcttctgcgt cgtttaattc gctaaaacca ttaccgattt
     8161 cacgaccacc gatgaagaat tcaaaacggt cggtgatttc cgggttaaca tcattacggc
     8221 gcgccagcgg tgacacttct gccggatatt ccgtaataaa ggttggctga atcagatgtg
     8281 cttctgccac ttcatcaaag atctccgtga taatacgtcc cagcccccag cttttctcta
     8341 ccgtaattcc gatagattca gctaatgctt tagcagcatc aaaattatcc aggtcggcca
     8401 tatcggtttc tggacgatat tttttgattg cttcgcgcat ggtgagtttt tcaaacggtt
     8461 tgccgaaatc aaacacatgc tcgccataag tgactttagt ggtacccaga acctcttgtg
     8521 ccagggtgcg gaacagtgac tctgtcagtt caatcaaatc gtggtaatcc gcatacgcca
     8581 tgtagagttc catcattgtg aactcaggat tatggcgaac agaaatacct tcattacgga
     8641 agttacggtt gatttcgaat acccgttcaa aaccgcctac aaccagacgt ttcagataca
     8701 gctccggcgc gatacgcaga tacatatcta aatccagagc attatgatgg gtaataaacg
     8761 ggcgagcaga tgccccacct ggaattacct gcatcatcgg ggtttctact tccataaagc
     8821 cgcgcgcgac catgaactga cggatagcgg ccagaatttt tgaacggaca acaaacgttt
     8881 gacgggattt atcgttagcg atgaggtcca gataacgttg acgataacgg acttcctgat
     8941 cctgcagacc atggaattta tctggtaaag gacgtagtgc tttagtcagc aggcgcaact
     9001 cagtacagtg aatggaaagc tcacccgttt gcgtcttaaa cagcgtaccg cgggcaccga
     9061 taatgtcacc cagatcccat tttttaaact gatcgttata aacaccttct ggcaggctat
     9121 ctcttgcaac gtacagttga atacggccac cgacatcctg caacgttaca aaggaggctt
     9181 tccccatgat acgacgggtc atcattcggc cagcaaccga gacttcaatg tttaaggatt
     9241 ccagttcctg gttattcttc gcatcaaact cttcgtgcaa ctggtcagag gtatggtcgc
     9301 ggcgaaaatc attgggaaac gccacacctt gctgacgtag tgccgccagt ttttcgcggc
     9361 gatttctcag ttcatcgtta aaatcaatag cctcattggc tccccgtgtt tcttgttcag
     9421 acattttggt tcctctaaat ccagctttca atttagcgta gataaagaga cagatcggta
     9481 cactgtatta tctgcctcga ctattaataa ctcagtttat cttatcgaaa ctaacgctta
     9541 cctcaagttg atatcactaa taaataacca tcaaaatggt ttaaatgact tattaatctc
     9601 gatttactag tgtgattaac tgaaagttaa tttattcata accaattgtt tttacgacag
     9661 ttaatcgttg ctctcctgta tcatattcgt cggcgtgcta aacagaaaac gggtagcaaa
     9721 ggccaatacg acgatgatcg cgacacaagc caacgtccac tctcccatct gagaaaagaa
     9781 tcgctggtaa gctgcaatta ccataccgct aatttgcgac tctgtcgtct gctgtgccac
     9841 gacgcctgcc agccagttgg cgaccgcgcc tgttgccagc atataaatac cggttaatac
     9901 gccagacatt ttcagacgcg tgatttgcgc aatcgccacc gggtcaataa agagttctgc
     9961 aaagcccatt agcgccaacc cggatatcat cacgcccatt gacgcttgac cgtcagccgc
    10021 tgcatgtcgg gcatcaaatg ccaacaacat aaagccacaa gccatcagca gtaagccaaa
    10081 ggcaaacttc agccagacgc gcaatgttga gttgccgcgg ctttctggcg acgccagcca
    10141 ggccagtaca accccagcga gcatcactgc aattgcattc accgactgga atagtgctgt
    10201 aggtacttca atattgaatg cctgacgatt cacaaagcga tcgataaaca agctgatggt
    10261 actgccgccc tgttgtgcca gtacccagaa caacgtcccg acaaacatca acaatacaat
    10321 ttgccaaaga gcacggcggt gttcggggaa tttgatcatc atgcgggcaa tgatttgtgc
    10381 ggcaatgagg caaacgatcg ccagcaaata tccggaccag tcgttctcca gcagcagggt
    10441 aaaaaatact ggggctaaac agagcatcac cactaaccag ctccatactg gtaaggcaaa
    10501 tttgacgcta gtgagcgctt ttttatccat actacgcgtg gactggaaat gacgatgacc
    10561 gcttaagaaa atcaacaaac cgataaacat gccgccaccc gcaagggcaa agccaacatg
    10621 ccatccatac cactgagcag ccaggccgca ggcgattggg gcggcgatag aaccgatatt
    10681 gcccgcagca tacagcagcg aaaaaccgcc atcacgtcga tgatcgttct cgtcgtagag
    10741 ctcgccaagc aaac
//
LOCUS       DAJTYZ010000133        10706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000133 DAJTYZ010000000
VERSION     DAJTYZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.133"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..716
                     /locus_tag="OO596_003347"
     CDS             <1..716
                     /locus_tag="OO596_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719129.1"
                     /translation="LSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMTEFDDNGKII
                     FNINALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGAL
                     SESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLA
                     SFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDK
                     WKRENMTPFMYVLNTPPVI"
     gene            726..1205
                     /locus_tag="OO596_003348"
     CDS             726..1205
                     /locus_tag="OO596_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719130.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(1342..2127)
                     /locus_tag="OO596_003349"
     CDS             complement(1342..2127)
                     /locus_tag="OO596_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719131.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(2457..2533)
                     /locus_tag="OO596_003350"
     tRNA            complement(2457..2533)
                     /locus_tag="OO596_003350"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2497..2499),aa:Asp,seq:gtc)
     gene            complement(2666..3397)
                     /gene="dnaQ"
                     /locus_tag="OO596_003351"
     CDS             complement(2666..3397)
                     /gene="dnaQ"
                     /locus_tag="OO596_003351"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6719132.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            3462..3929
                     /gene="rnhA"
                     /locus_tag="OO596_003352"
     CDS             3462..3929
                     /gene="rnhA"
                     /locus_tag="OO596_003352"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6719133.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(3926..4648)
                     /locus_tag="OO596_003353"
     CDS             complement(3926..4648)
                     /locus_tag="OO596_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6719134.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            4682..5437
                     /gene="gloB"
                     /locus_tag="OO596_003354"
     CDS             4682..5437
                     /gene="gloB"
                     /locus_tag="OO596_003354"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6719135.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5509..6867
                     /gene="mltD"
                     /locus_tag="OO596_003355"
     CDS             5509..6867
                     /gene="mltD"
                     /locus_tag="OO596_003355"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6719136.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(6916..7512)
                     /locus_tag="OO596_003356"
                     /pseudo
     CDS             complement(6916..7512)
                     /locus_tag="OO596_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(7543..8343)
                     /locus_tag="OO596_003357"
     CDS             complement(7543..8343)
                     /locus_tag="OO596_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6719137.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            8584..9498
                     /locus_tag="OO596_003358"
     CDS             8584..9498
                     /locus_tag="OO596_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6719138.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(9495..10298)
                     /gene="dkgB"
                     /locus_tag="OO596_003359"
     CDS             complement(9495..10298)
                     /gene="dkgB"
                     /locus_tag="OO596_003359"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6719139.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(10461..10537)
                     /locus_tag="OO596_003360"
     tRNA            complement(10461..10537)
                     /locus_tag="OO596_003360"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10501..10503),aa:Asp,seq:gtc)
BASE COUNT         2951 a         2450 c         2429 g         2876 t
ORIGIN      
        1 tactcagccc aataacatca tttgattaca ttcattttat cactacacat ccttcaggta
       61 taaaagatac actcgcctgg ttagttaacg cgagtaaatt aatgactgag tttgatgaca
      121 atgggaaaat tatatttaac attaatgcct taaagtatac aaaagcaagt tactttgaaa
      181 tacttggaga gaaatatata aaaatcacaa catccagccc ctggctttta gaaaagctcg
      241 gtaaatatat tttctcttca cgagcgcctc aagttctgga actcgctatt ggctggcgag
      301 gagctttatc tgaatcaata aaaggagtta agttctgtat ctggttttca gtagcctgga
      361 gaaccataga atttattatg agttcagagc gagatctggt taattttctt ggcgattttt
      421 caatggatgt ggccaaagca gttatagccg gtggtgttgc aaccgctatt ggaagcctgg
      481 cttcttttgc ctgtgttagc tttggctttc cagtaattct tgtcggagga gcaattttac
      541 tgacagggat agtgtgtaca gttgttttaa atgaaatcga tgctcaatgc catttatcag
      601 aaaaattaaa atatgcaatt agagatggac taaaacggca acaggaactt gataaatgga
      661 aaagggaaaa catgactcca tttatgtatg ttcttaacac tccacccgtg atatgaggat
      721 taaccatgcg tttacatgtt aaattaaaag agtttctttc gatgtttttt atggccatat
      781 tatttttccc tgcattcaat gcttctttgt tttttaccgg agttaaacct ctatattcaa
      841 taataaaatg ctccacggaa atattttatg attggagaat gttgattctg tgctttggtt
      901 tcatgtcgtt ttccttttta aatattcatg taattttact tacgataata aaattattct
      961 taatcaaaaa aacaaaggta gtcaatttcg ccacagacat aacaatacag ctaactctta
     1021 tatttttact aatagctatc gtaatagcac ctttaatagc tccttttgtc acaggatatg
     1081 tcaatgctaa ttatcacccc tgcggtaata atacgggaat tttcccagga gcaatctata
     1141 ttaaaaatgg catgaaatgt aataatgaat atatttctcg caaggaagat tcagcagtta
     1201 aataattaaa cattctaaaa caaacagttt ttaactccgg attccatgtc ctttcttgca
     1261 ctgtctttgt caagaaaaat ttcttatgca aagaattttg tattatttcc gccagaaatt
     1321 cctggcggca gatataattt attattgata gcggttaacc aaatctttta ccatcgcttt
     1381 ataggtcttt tgcttaagtt gcatgatttg ctgctctgtc atatcagtcg tactggcttc
     1441 attcttgtcg ggcaatggtg gaagcacttt atattcataa ttcaccggcg accaggcagc
     1501 ccactcacct gtagctacat ccaccagcgt gaatcgtacc agatagcgca gtgacatcgt
     1561 gtcagtcaat ttttgatttt tataatctga ccaaactatt gatttcaatc cagagtcata
     1621 ttttcctgtc tgcaacgtat cctggaaaac aataatcgct ttctgatgcc cttttgcagc
     1681 cacaaaacgc agtgcctgca tccagttcat attctcagca ctggcaacat cctcgttctc
     1741 atttttatct ttgtttttgt tacaagtcag aggcttttgc ctgtccggga taccagagaa
     1801 tgtggaaaca gtataatatt ttcgcatctc ctcctgcata atggtttccg gtgcgcggtt
     1861 gccggattga acaagtatta caggagaatt aaggggaacg cggaactcat ctccatcaag
     1921 cgcagcttga atatcctctt cggatacggc cagagtggtt tcattaccga ataaatcttt
     1981 gtctgtcagt tgctggggag tgtttttatt aatattactt tgtgtgactt gagctttttt
     2041 cttcttttca tcaatagaag attcagagac acatcctgca agcagcgaga ataatacaca
     2101 tatacaacaa acccttctta aattcataat cggacactcc ctcgcctgtc ataatcatgg
     2161 taatgtttag ctaacgattt ttaattatat actccacttg aatcagaaca aacaaaaata
     2221 acagtatata taaattcatt aaatcaatag aaaaatgaaa atcataacat tcaaatatat
     2281 ttaaattgaa tgatttgata gtatgattaa atattaatga tatggtctac catatgtgat
     2341 taacacattt acagcataac aaaaccatag accgaagaaa tagcgggatt taaatgcaaa
     2401 aaaaccgcaa aattaaaaat cttgcggttc tctgaactca ttttcatgag tgaatttggc
     2461 ggaacggacg ggactcgaac ccgcgccccc ctgcgtgaca ggcaggtatt ctaaccgact
     2521 gaactaccgc tccgcgttgt gttccgttgg gaacgaggcg aatattacgg attgcctcac
     2581 ccttcgtcaa cggtttttct catcttttga atcgtttgct gcaaaaatcg cccaagccgc
     2641 tatttttagc gccttttaca ggtatttatg cccgccagag gcagcttccg cccttcttct
     2701 ccaccagatc aagacgggct tcatgagctg caagctcttc atctgtcgca aaaacaacgc
     2761 gtaacttact tgcctgacgt acaatgcgct gaattgttgc ttcaccttgt tgctgctgtg
     2821 tctctccttc catcgcaaaa gccatcgacg tttgaccacc ggtcatcgcc agataaactt
     2881 ccgcaaggat ctgtgcatcg agtaatgccc cgtgtagcgt tcgtttactg ttatctattt
     2941 cgtagcgggc acataacgca tcgaggctgt tgcgcttacc aggaaacatt ttcctcgcca
     3001 ccgcaaggct atcggtgacc ttacagaagg tattggtctt cggaatatcg cgcttaagca
     3061 acgaaaactc gtagtccata aagccgatat cgaacgctgc gttatggatc accaactccg
     3121 cgccgcggat atagtccatg aactcatcgg ctacttcggc aaacgtgggc ttatcgagca
     3181 aaaattcatc ggcaatacca tgtacgccaa aggcttccgg atccaccagc cgatcgggtt
     3241 tgagataaac atggaagtta ttgcccgtca ggcgacggtt caccacttca acggcaccaa
     3301 tctcaatgat cttgtggcct tcatagtgcg caccaatctg gttcataccg gtggtttcgg
     3361 tatcgagaac gatctggcgt gtaattgcag tgctcatagc ggtcatttat gtcagactta
     3421 tcgttttaca gttcgattca attacaggaa gtctaccaga gatgcttaaa caggtagaaa
     3481 ttttcaccga tggttcgtgt ctgggcaatc caggacctgg gggttacggc gctattttac
     3541 gctatcgcgg acgcgagaaa acctttagcg caggctacac ccgcaccacc aacaaccgta
     3601 tggagttgat ggccgctatt gtcgcgctgg aggcgttaaa agaacattgc gaagtcattt
     3661 tgagtaccga cagccagtat gtccgccagg gtatcaccca gtggatccat aactggaaaa
     3721 aacgtggctg gaaaaccgca gacaaaaaac cagtaaaaaa tgtcgatctc tggcaacgtc
     3781 ttgatgctgc cctagggcaa catcaaatca aatgggaatg ggttaaaggc catgccggac
     3841 acccggaaaa cgaacgctgt gatgaactgg ctcgtgctgc ggcaatgaat cccacactgg
     3901 aagatacggg ctaccaagtc gaagtttaag cttgtggttt acgacattgc cgggtggctc
     3961 caaccgcctg gcgaattcgt ggcttgtttt tactctgttt catcgggttt agcgttaaag
     4021 gaatagtccg tttccgggca acaataagtt gtaagcaacc aagcgcagga atatgcgcat
     4081 tcaatagttt tcctccgtgt ttgttccacg ggagaacgtg gaaacggctg gcgtgcagca
     4141 cttcaaaatt caacaaagag agccagtcca gttgccgcat cagagtaaac atccggctgt
     4201 tatagggcga ggtttttcgc aataccggca caagtttgcg taatcccatt aaactaatgg
     4261 gattgaagcc actaatcacc agccagccat catcaatcaa tacccgatcg gtttcacgca
     4321 ataaacgatg cggatcggtg caccacggca atgtatgtgc cagtagacaa acatcaacgg
     4381 atttatcggc aaaaggaaga tgaagtggat ccgcctgcac ctggacgggc attccttgcg
     4441 cagaaacatt cacttgatga gaaaccgcgc acgcttcgca attgatttct gcgcttaaat
     4501 tgccaatctt aagcagatga aaaccataca ttttagtgaa ccacgggttg agctggcgct
     4561 ccagcgcctt gcgataaagc tctccccagg gcaaatcgcc ccagcgatca ggagccacga
     4621 cagtttgagg gactcttgcc ggtttcatca caaccttccg tttcacacta agaggtaatc
     4681 tatgaatctt aacagtattc ccgcctttga tgacaattac atctgggttt tgaatgatga
     4741 agcaggtcgc tgcctgattg tcgatcccgg agacgcagag ccagtattaa acgccattgc
     4801 cgccaataac tggcaaccgg aggccatatt tctcacccac catcatcacg atcacgttgg
     4861 cggcgtaaaa gaactggtgg aaaagtttcc gcaaattgtg gtgtatggtc cacaagagac
     4921 acaagataag ggaacaacac aggtagtcaa agatggcgaa actgccttcg ttttggggca
     4981 tgaatttagt gtaattgcca caccgggtca cactttagga catatctgtt acttcagtaa
     5041 accttatcta ttttgcggcg acacgctgtt ttctggtggg tgtggtaggt tgtttgaagg
     5101 gacagcatca caaatgtatc aatcacttaa taagttaagt gcgttacctg acgatacatt
     5161 ggtatgttgt gctcatgaat atacgttatc aaatatgaag tttgctttga gtattcttcc
     5221 gcacgatttg tccataaatg attattatcg taaagttaag gagttacggg caaaaaatca
     5281 aataacacta cccgtaattc tgaaaaatga gcggcaaatt aatgtttttt taagaacgga
     5341 agatattgat ttaattaatg taattaatga agaaacatta ttgcaacaac ctgaagagcg
     5401 ttttgcatgg ttaaggtcaa agaaagatag gttctgataa aactttcttg tcatcggctc
     5461 cgttcgccgt tatgatcggt cgtcttttaa gcaactattg acacacacat gaaggcaaaa
     5521 gcgatattac tcgcctctgt cctgctcgtg ggttgccaga gtaccggcaa cgttcaacag
     5581 cacgcacaga gcctttctgc agctggtcaa ggggaagcag caaagtttac aagtcaggca
     5641 cgatggatgg acgatgggac gtctatcgcg ccagatggtg acttgtgggc tttcattggc
     5701 gacgagctaa agatgggaat tccggaaaat gaccggattc gcgaacagaa acagaaatat
     5761 ttacgcaata agagctatct ccacgatgta actttacggg cagagccgta tatgtactgg
     5821 atagcagggc aagttaaaaa acgtaacatg cctatggaac tggtactact acccatagtg
     5881 gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg ccgcaggcat ctggcagatc
     5941 attccgagca cggggcgcaa ttatggtttg aaacagaccc gcaattatga cgcgcgtcgc
     6001 gatgttgttg cttcaacaac tgccgcgctg aatatgatgc agcgtctgaa caaaatgttt
     6061 gacggcgact ggcttctgac cgtagcggct tataacagcg gcgaaggtcg ggtcatgaag
     6121 gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact tctggtcgtt accgttgccg
     6181 caggaaacga agcagtacgt gcctaaaatg ctggcattga gtgatattct caaaaacagc
     6241 aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc gtgctctggc gcgtgtgcac
     6301 ctgagtagcc cggttgaaat ggcgaaggtt gcagatatgg cggggatttc cgtcagcaag
     6361 ctgaagacat tcaacgctgg cgtgaaaggc tccacgctgg gcgcaagtgg gccgcagtac
     6421 gtgatggtgc caaagaagca tgcagatcaa ctgcgtgaat ctctcgcttc aggcgaaatt
     6481 gctgctgtac agtcgacgct ggttgccgac aatacgccgc ttaacagccg tgtttacacc
     6541 gtacgctctg gcgacacgct ttcaagcatc gcttcacgtc tcggcgtaag caccaaagat
     6601 ttgcagcagt ggaacaaact gcgcggatct aagctgaagc caggccaaag tttgacgatt
     6661 ggtgcaggca gtagcgcaca gcggttggca aacaacagcg atagcattac gtatcgtgtg
     6721 cgcaaaggcg attcgctttc aagcattgct aaacgccacg gcgtgaacat caaagatgtg
     6781 atgcgctgga acagcgacac tgcgaatctg caaccaggcg ataagctgac gttgtttgtg
     6841 aaaaacaaca acatgccaga ttcctgacaa accagataat aaaaaaggca ccgattcccc
     6901 cggtgccttt tttatttatg ccgctttatg tgcttctacc atgatgatat cactggtgaa
     6961 agagccatca gtctgcaagg aaaaatacgt tctcacctct gtcgatgcgc tctgttggta
     7021 aatgcgaata gcgtctacta acgcttctgg cgtacgcatt ctcacgaccc atgaagaaaa
     7081 ttccagcggt aacttatctg taattaaatt atcaactatc agattggctt cattgattaa
     7141 cgtcaaccac gcaccgctgg cgtagtttcg tacgtgagag gtatcgcgta atgcttctac
     7201 cgcctgtaac cagatgtcgc gcactgggtg acccggagac attacatcca tcacaatcag
     7261 cctaccgcca ggtttcaata tcctattcac ttctcgcagt gctgcaccaa catcatgcca
     7321 gtgatgggca gaataacggc tgataacaat atcaaatgcg ttatcggcaa atggcagact
     7381 tgcggcatat ccctggcggg tggtgatatt tttcagttgc cgggcttcgg cggcttgtgc
     7441 cacgacatcc agcatgtggg cagataagtc atacgccacc accgcgctca cgttttgcgc
     7501 agcgccaaac gttggtctgc cctgcggcag acctgacata ccttatttat caggcttgcc
     7561 gggactgaat tcaacgagta gcggattgtg atcggaagcg cgcgtaacca gtacagaagc
     7621 ttcactgacg ttcagaccac ggtagaaaac aaaatcgagc gggcgaccaa acgcccgacg
     7681 gcgctgatca tcggtaaaac gcacctggcg cagcgacatt tcccgcgcaa agcgatataa
     7741 cgcgttcatc cttctacggc tccaggcatt gaaatctccc gccataatga ccgggccgct
     7801 gtggtgagct atctgatcac caataggaag taactgctta ctatagacat ccacgcccag
     7861 actgaagttg acggcgtgta tattaaccac catcaacagg cgggtgtcag gtaatggata
     7921 gaccgtcacc agtgccgact tcgccagacg caaaatgggt tctcgttcac gtaacgggca
     7981 gcaatacact ggatgtgccg ccgaaagggt cattacgcca gaaggatgtt gtggcagcac
     8041 gaaagcgggt acctgatcgg cggcaagata gttagcggtc gcaaactgta ccaactctgg
     8101 cgttgtttgc gcttcctgca ataacaccag atgtgcgtct ttgccgtaat tctttaatac
     8161 cgacaaccat tcagcgcgtt gctgtttgta tatgttccac accaggatcc gaatacgctc
     8221 ttcggtactt aacggttctc cgggtggtaa tgcctggccg atgctcgcaa aagaccccgg
     8281 cggtaagatc ctctccgcag gttgtccggc aacatagcgc atggcatagg tgttttttcg
     8341 cacttttgac tttgaaacct ctattacgtc aacccttccg cactcgggag ggttctactg
     8401 ttatagggat tttagctcac tctttcaacg agcaattgta tattgttatg taagcaagtg
     8461 cttacaccgg tcagaaattt gctcattatt gagaacatta aatcggatga catatcacgc
     8521 atcctgatgg tttaatgaca aataaattca aaatatctca tctattttgc aacaggagca
     8581 aaaatgaaag ccacgtcgga agaactcgcc atttttgttt cggtcgtcga aagcggcagc
     8641 tttagccggg cagcggaaca attagggcaa gcaaactcag cggtaagccg ggcggtgaaa
     8701 aagctggaaa tgaaacttgg cgttagcctg cttaatcgga ccacgcggca acttagcctg
     8761 acggaagaag gcgagcgtta ttttcgtcgc gtacagtcaa ttttgcagga gatggcagcg
     8821 gcagaatcag aaattatgga gacgcgtaat acaccgcgtg gactgttacg gatcgatgcc
     8881 gcaactccag tggtgctgca ctttctgatg ccgttaatta agcctttccg tgaacgctat
     8941 ccggaagtca ctttatcgct agtctcctcc gaaacgatta ttaatttgat cgaaagaaaa
     9001 gtggatgtcg cgatacgcgc tggtacgtta acggactcca gcttacgtgc caggccgtta
     9061 tttaacagtt atcgaaaaat tatcgcctcc cccgattata tttcccgcta cgggaagcca
     9121 gaaacgatcg acgatttaaa gcaacatgtt tgcctgggat tcactgaacc cgcttccctc
     9181 aatacctggc cgatagcctg tagtgatgga caattacatg aggtgaagta cggtttgtca
     9241 tccaatagtg gggaaacact gaaacagctt tgcctgagtg gcaacgggat tgcgtgtttg
     9301 tccgactaca tgatcgacaa agaaatcgct cgcggagaat tggtggagtt aatggcagat
     9361 aaagtgttgc cagtggaaat gccattcagt gccgtctatt acagcgaccg tgcggtaagt
     9421 acgcgcatcc gggcttttat cgatttcctt agcgagcatg taaaaacagc tcccggagga
     9481 gctgtcagag aggcttaatc ccattcagga gccagacctt ccgggctaac caggcggtcg
     9541 ttgcaatcca gtgcggcgat cgctttttta tcttcggcat caagctgtaa attttgtgcc
     9601 ttaagattac tttccaggtt tttacgttta gtagaagaag gaattactga gtaaccttcc
     9661 cccatagccc acgccagaat cacttgtgcc ggagtcgcat tgtgtttagc tgcaatacga
     9721 gcaataacct catctttcag tgccttacca taagccagcg tcatatagga agtgatatga
     9781 ataccgtgct gtttagccca ggcaaccact ttacggtttt gcagataagg agagagttca
     9841 atctggttgg tagcgatgtt ttcagcgcca acagcagcaa tcgctttttc catcaacggg
     9901 atcgtgaagt tggaaatacc gatctcacgt gtcagacctt gttttttggc ctccagcagc
     9961 gcctgcataa actcttcaac agagacttca tcgtttggtg acggccagtg gattagcgtc
    10021 agatcaacat aatcggtacg caatttttgc aggctctctt ttagactcgg gatcaatttg
    10081 tctttgctga gattttcaat ccagatttta gtggtgatgt agagttcatg acgtggcacg
    10141 ccactttctg caatcgcctg acctactgcg gcttcgttat catagatttg tgcagtatca
    10201 attgcgcgat aaccaagttc aagcgccgtt ttcacagatg aaataacaac gtcgtctttg
    10261 agacggaaag tacctaaacc aaatgcaggg atagccataa tatgcctctt ttaatgctat
    10321 tttcgttaac tgaggtgatt atgggcttca gactgttgcg gaaaaagagc ataaaatgca
    10381 gaggattttt gcgattctgg caataataga tatacagacg aaaaaaaacc tcgagcgtta
    10441 actcgaggct tcttaataag tggcggaacg gacgggactc gaacccgcga ccccctgcgt
    10501 gacaggcagg tattctaacc gactgaacta ccgctccacc gaattctttt acaaccaccg
    10561 gttttatgac cggcttactg cttaatttga tgcctggcag tttatgaatc acttcgggct
    10621 gttgcttcgc aacgttcaaa agctttcgct tttgtcctac tctcttatcg agtagtgaac
    10681 tgcctcaaag gtcagtgcca ttcttc
//
LOCUS       DAJTYZ010000134        10687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000134 DAJTYZ010000000
VERSION     DAJTYZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.134"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..784)
                     /locus_tag="OO596_003361"
     CDS             complement(<1..784)
                     /locus_tag="OO596_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719140.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPESGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="OO596_003362"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="OO596_003362"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6719141.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="OO596_003363"
     CDS             1860..2747
                     /locus_tag="OO596_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6719142.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="OO596_003364"
     CDS             2780..3790
                     /locus_tag="OO596_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR6719143.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="OO596_003365"
     CDS             3860..5293
                     /locus_tag="OO596_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6719144.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="OO596_003366"
     CDS             5392..6741
                     /locus_tag="OO596_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6719145.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="OO596_003367"
     CDS             6731..7618
                     /locus_tag="OO596_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6719146.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="OO596_003368"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="OO596_003368"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6719147.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="OO596_003369"
     CDS             8352..9224
                     /locus_tag="OO596_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6719148.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="OO596_003370"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="OO596_003370"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6719149.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="OO596_003371"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="OO596_003371"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6719150.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2532 c         2854 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgctctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtc
//
LOCUS       DAJTYZ010000135        10592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000135 DAJTYZ010000000
VERSION     DAJTYZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.135"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(137..1165)
                     /gene="malI"
                     /locus_tag="OO596_003372"
     CDS             complement(137..1165)
                     /gene="malI"
                     /locus_tag="OO596_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6719151.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1340..2932
                     /gene="malX"
                     /locus_tag="OO596_003373"
     CDS             1340..2932
                     /gene="malX"
                     /locus_tag="OO596_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6719152.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2942..4114
                     /gene="malY"
                     /locus_tag="OO596_003374"
     CDS             2942..4114
                     /gene="malY"
                     /locus_tag="OO596_003374"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6719153.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4218..5219
                     /gene="add"
                     /locus_tag="OO596_003375"
     CDS             4218..5219
                     /gene="add"
                     /locus_tag="OO596_003375"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6719154.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5255..6295)
                     /locus_tag="OO596_003376"
     CDS             complement(5255..6295)
                     /locus_tag="OO596_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6719155.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6539..6664
                     /gene="blr"
                     /locus_tag="OO596_003377"
     CDS             6539..6664
                     /gene="blr"
                     /locus_tag="OO596_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6719156.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6929..7144
                     /gene="ydgT"
                     /locus_tag="OO596_003378"
     CDS             6929..7144
                     /gene="ydgT"
                     /locus_tag="OO596_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6719157.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7230..7670
                     /locus_tag="OO596_003379"
     CDS             7230..7670
                     /locus_tag="OO596_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6719158.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7747..8328
                     /gene="rsxA"
                     /locus_tag="OO596_003380"
     CDS             7747..8328
                     /gene="rsxA"
                     /locus_tag="OO596_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6719159.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8328..8906
                     /gene="rsxB"
                     /locus_tag="OO596_003381"
     CDS             8328..8906
                     /gene="rsxB"
                     /locus_tag="OO596_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6719160.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8899..>10592
                     /gene="rsxC"
                     /locus_tag="OO596_003382"
     CDS             8899..>10592
                     /gene="rsxC"
                     /locus_tag="OO596_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6719161.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGTCAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         2430 a         2736 c         2703 g         2723 t
ORIGIN      
        1 gtctcaagtt gtcagaatta aacacataaa cctcctgtga acttcagtaa aaataaaagg
       61 caccgcctgt gtgccacatt ttttatcatt aaccttgagg aaaggacact accaggtata
      121 gcagataaag agaggattat ttcgctgcaa tgagccgggc gggaataata agattgcgtg
      181 aatgcgtctc ttcatgggtg attttttgca tcatgcgatc cgcaagtgtg gtaccaagtt
      241 cccgcgcagg cgtgctggcc caggtaacgg gtatatcatc aagtgtggtt ggtgtcgcat
      301 cggtaaatgc cgccagcgaa acttgttgct caaagtaacg atcgactccg ctttccccgc
      361 tttgccgccc tgctttcagc aaaccaaacc atgcccccat cgcaatagtt tcgttatagc
      421 aaaccacagc actgatggtc gggttatgac gtaaaagcgc cgtgatagct tccgcggctt
      481 gcttctggct ggatgtgcac tccagcaccc aatcgctgtg aaacggcagg ccaaatttta
      541 gtagagttgc acaatagcct cccacccgtt ctgcccgggt taatgaggaa ctttgccctc
      601 ccagccaggc gatccgctga tgcccattgc gaatgagatg ctccgtcaac aactgtgcag
      661 cctgcatgtt gtccgggcga accgtatcaa catcatcaag ataactggca cgggaagcga
      721 aaatcaccgg gatagctttt tcttctgcca tccgtcgcag gtcatcgctg cttcctgcag
      781 ccccggcaat taccacacca tcgacaccct gattcagtaa cagtgaaaac cgctgtgcca
      841 gctgctcgcc gtctttaccg ccgtgaagca aaaaaaccat ccgtccctgc gcttccagag
      901 cttccgtcaa tccggccgtc aattcggcgt aaaacggcgc agataaatca cggacgatca
      961 aaccaatgac gccgctttgc ccgccgcgca gcgccgacgc ctggcgattg cgcacaaatc
     1021 ccagctcttc aatggcggcg ttaacgcgtt ctcctgtggc ggtagagatt cgccctttgc
     1081 cactaagcac cagcgaaacg gtacttaccg acacgcccgc agccagcgca acatcatgaa
     1141 tggttatttt tttggcggta gccatgcaga tgacctactc cctgattatg tgacagataa
     1201 aacgttttac cttttattta tcttataccc gctattatcg ttgcgtaatg tgatttatgc
     1261 ttcactaaaa tttgataaaa cgttttatct tctcgcgcaa tttactgaat ccagattgtt
     1321 ctctacgagg agtcgtttta tgacggcgaa aacagcaccg aaagtcacgc tgtgggagtt
     1381 cttccagcag ttaggcaaaa ccttcatgtt acccgtggca ttattgtcgt tctgcggcat
     1441 tatgctcggc attggtagtt ctcttagtag ccacgatgtc atcaccctga tcccggtcct
     1501 gggcaacccc gtgttgcagg ctatctttac ctggatgagt aagattggct cgtttgcttt
     1561 tagtttcctg ccagtcatgt tctgtatcgc catcccgctg ggtctggcac gcgaaaacaa
     1621 aggcgtagcg gcattcgctg gcttcgtcgg ttatgcggta atgaacctcg cggtaaactt
     1681 ctggttgacc aataaaggca ttctgccaac cacggacgcc gcggttctga aagccaataa
     1741 catccagagc attcttggga tccagtcgat cgacaccggg atcctcggtg cggtgatcgc
     1801 cggtattatc gtctggatgc tgcatgagcg attccataat atccgcctgc cggatgcgct
     1861 tgcattcttc ggcggtacgc gcttcgtacc aatcatctcc tcgctggtga tgggccttgt
     1921 cggcctggtg attccattag tctggccgat tttcgcgatg ggtattagcg gcttgggcca
     1981 tatgataaac agcgcgggtg atttcggacc gatgctgttt ggtaccggtg aacgtctgct
     2041 gttgccgttt ggtctgcatc acattctggt ggcattaatt cgctttaccg acgcaggcgg
     2101 cacgcaggaa gtctgcggtc aaaccgtcag cggcgcattg accatcttcc aggcgcaatt
     2161 gagttgcccg accactcacg gtttttctga aagcgccacg cgtttccttt cgcaaggcaa
     2221 aatgcctgcg tttctcggcg gtctgccagg tgcagcgtta gcgatgtatc actgcgcgcg
     2281 cccggaaaat cgccataaaa ttaaagggct gctgatttct ggcctgatcg cctgtgtcgt
     2341 tggcggcacg accgaaccgc tggaattcct gttcctgttc gtggcgccag ttctgtatgt
     2401 catccacgcg ctgttaaccg gcctcggctt cactgtcatg tctgtgctcg gcgtcaccat
     2461 cggtaatacc gacggcaata tcatcgactt cgtggtgttc ggtattttgc atggtctgtc
     2521 aaccaagtgg tacatggtgc cagtggtggc ggcaatctgg tttgtcgttt actacgtcat
     2581 cttccgtttc gctatcaccc gcttcaatct gaaaaccccg gggcgcgata gcgaagtggc
     2641 cagttcaatc gaaaaagccg ttgccggtgc gccgggtaaa tcaggttaca acgttccggc
     2701 aatcctcgaa gccttaggcg gtgccgacaa tattgttagc ctcgataact gcattacccg
     2761 tctgcgtttg tctgtgaaag atatgtcgct tgttaatgtg caggcactga aggacaatcg
     2821 ggcaattggc gtagtacaac ttaatcaaca taacctgcag gttgttatcg ggccacaagt
     2881 tcagtcagta aaagatgaaa tggccggtct gatgcatact gtccaggcat aaggataaga
     2941 tatgttcgat ttttcaaagg tcgtggatcg tcatggcaca tggtgtacac agtgggatta
     3001 tgtcgctgac cgtttcggca ctgctgacct gttaccgttc acgatttcag acatggattt
     3061 tgccactgcc ccctgcatta tcgaggcgct gaatcagcgc ctgatgcacg gcgtatttgg
     3121 ctacagccgc tggaaaaacg atgagtttct cgcggctatt gcccactggt tttccaccca
     3181 gcattacacc gcaattgata cgcagacggt ggtgtatggc ccttctgtca tctatatggt
     3241 ttcagaactg attcgtcagt ggtctgaaac aggtgaaggc gtggtgatcc acacgcccgc
     3301 ctatgacgca ttttacaagg ccattgaagg taaccagcgc acagtaatgc ccgttgcttt
     3361 agagaagcag gctgacggct ggttttgcga tatgggcaag ctggaagccg ttttggcgaa
     3421 accagaatgt aaaattatgc tcctgtgtag cccacagaat cctaccggga aagtgtggac
     3481 gtgcgatgag ctggagatca tggctgacct gtgcgagcgt catgatgtgc gggttatttc
     3541 cgatgaaatc catatggata tggtctgggg cgagcagccg catattccct ggagtaatgt
     3601 ggcgcgggga gactgggcgt tgctgacgtc gggatcgaaa agtttcaata ttcccgcgct
     3661 gaccggtgct tacgggatta tagaaaatag cagcagccgt gatgcctatt tatcggcact
     3721 gaaaggacgt gatgggcttt cttccccttc ggtactggcg ttaactgccc atatcgccgc
     3781 ctatcagcaa ggtgcaccgt ggctggatgc cttacgcgtc tatctgaaag ataacctgac
     3841 gtatatctca gataaaatga acgccgcgtt tcctgaactc aactggcaga tcccgcaatc
     3901 cacttatctg gcctggcttg atctacgtcc gttgaatatt gacgacaacg cgttgcaaaa
     3961 agcgcttatc gagcaagaaa aagtcgcgat catgccgggg tatacctatg gtgaagaagg
     4021 tcgtggtttt gtccgtctca atgccggctg cccacgttcg aaactggaaa aaggtgtggc
     4081 tggattaata aacgccatcc gcgctgttcg ctaaccccaa ttgcgcaacg taaaaaatcg
     4141 ttgcgcaatc gtggattttt accctgcttt gtttttataa tggtgcgcac ttttatatcc
     4201 agaaaaagag tgcgaccatg attgatacca ccctgccatt aactgatatc catcgccacc
     4261 ttgatggcaa cattcgtccc cagaccattc ttgaacttgg ccgccagtat aatatctcgc
     4321 ttcctgcaca atccctggaa acactgattc cccacgttca ggtcattgcc aacgaacccg
     4381 atctggtgag ctttctgacc aaacttgact ggggcgttaa agttctcgcc tctcttgatg
     4441 cctgccgccg cgtggcattt gaaaacattg aagatgcagc ccgtaacggc ctgcactatg
     4501 tcgagctgcg tttttcacca ggctacatgg caatggcaca tcagctgcct gtagcgggtg
     4561 ttgtcgaagc ggtgatcgat ggcgtacgtg aaggttgccg cacctttggt gtgcaggcga
     4621 agcttatcgg cattatgagc cgaaccttcg gcgaagccgc ctgtcagcaa gagctggagg
     4681 cctttttagc ccaccgtgac cagattaccg cacttgattt agctggtgat gaacttggat
     4741 tcccgggaag tctgttcctt tctcacttca accgcgcgcg tgatgcgggc tggcatatta
     4801 ccgtccatgc aggcgaagct gccgggccgg aaagcatctg gcaggcgatt cgtgaactgg
     4861 gtgcggagcg tattggacat ggcgtaaaag ccattgaaga tcgggcgctg atggattttc
     4921 tcgccgagca acaaattggt attgaatcct gtctgacctc caatattcag accagcaccg
     4981 tagcagagct ggctgcacat ccgctgaaaa cgttccttga gcatggcatt cgtgccagca
     5041 ttaacactga cgatcccggc gtacagggag tggatatcat tcacgaatat accgttgccg
     5101 cgccagctgc tgggttatcc cgtgagcaaa tccgccaggc acagattaat ggtctggaaa
     5161 tggctttcct cagcgcagag gaaaaacgcg cactgcgaga aaaagtcgcc gcgaagtaac
     5221 aaaaatggat ggtgcaattt gcaccatcca tttttcacgc aaggcacaac gtcgcgcgat
     5281 gtttggcgga ttcgatgccc agctcaatca actccattac ctggattgcc tggcttgccg
     5341 gaaccggatt ttcaccatcg ccatttaacg catcacgaat cgccgcatag taagccggat
     5401 aattcccagg caccgtcaac agcgtttctt cgacacgttc ctcaccttcc acgcgggtaa
     5461 gtacgccatc acgcatatcg tagccccagt cttcctgcgg cagacgctcg ccatttttca
     5521 gacgttcttc ctgtggatcg aggccatatt tcacacaact gcctcgggat ccatgcacga
     5581 tataccgtgc tgactcagcg gctgccagca tggtaccgtg taaaatgact cgccgctgtg
     5641 gataggacaa gatggcgtgg aaataatcgg tcgactgcgc tccgggccgt aactgtgcca
     5701 aatctaccgt catgctgacc ggtaaaccaa atagcgtaat ggcctgatca agaagatgcg
     5761 gtgctaaatc gtaccagata ccgctgcctg ggccgccctg ttcacgccaa cgatcgcgca
     5821 cctgcggacg gaagcggtca aaatgagact caaagtaagc aacttcaccc agcacgcctt
     5881 ccgcgagcaa accttttagc gtcaagaaat cgctatccca acgacggtta tggaatacag
     5941 acagcacacg ccccaggctt tttgccagcg catccagctc tcgcgcttgt gacagtgtca
     6001 cggtaaaggg tttatcaacg accacatgtt tacccgcctc aagcgccgct ttggctaacg
     6061 ggaaatgggt atcgttgggt gtaggaatga caatcaggtc tatgttggga tcgttaaaca
     6121 gatgcttcgg ctcagagaca accgtaaccg ttggccagtc ggcttttact tttgtttcat
     6181 cactgctgga gattaccgcc agttccagcc cgggcgtgcc cgcaatcagg ggcgcatgga
     6241 aggttttgct cgcataacca tacccaatca acccaacacg gatgttgtcg ctcatgtcat
     6301 tgcctctcat tgaagtatga tggctatttg acactatcct ttacccacgc tcaacagttt
     6361 aataacctgc cagcaataag ggatgttgtt taacttaagt caaaaaaaat agcgaatttt
     6421 ccaacgacaa aagctaaata tcgcaaaaac ctcagtaaaa atcttgctgg agctattatt
     6481 gctaagtaac atttaccccc tgaagttaat ggatcaatca agagagatgt gggctgtaat
     6541 gaatcgtctt attgaattaa caggttggat cgttcttgtc gtttcagtca ttcttcttgg
     6601 cgtggcgagt cacattgaca actatcagcc acctgaacag agtgcttcgg tacaacacaa
     6661 gtaagctctg cacttgtgga gcgacatgct gcccgtccgg gtgcatgttt tcacttgtcg
     6721 gatattaaac caggaattta ttatcttgtt cgatgttgtt ggtgattgtc agggatagta
     6781 aagcgcgtta ctcttttcag agcagttttt catccctgaa tatttgccat cagcgcattc
     6841 ttattattgt aataacacaa gcgttcactt ttggtgaaat tttatcatca ctctacccct
     6901 ttggtattaa ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg
     6961 caaaatcagt tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac
     7021 ggacgatcag gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt
     7081 ttctggcggg cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca
     7141 ataaagaaag tagcgatcgg ttataaaagc cttataataa cgcctctaaa attagatttg
     7201 cctgtttgcg tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag
     7261 gctggttgct tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt
     7321 tgttgctgta taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag
     7381 cactgacgac acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt
     7441 acacactgtg gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata
     7501 ttatctggct gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg
     7561 aagatggcat agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc
     7621 cctatttcaa acgctcgtcg cgggtaaaag cgacgtttgt gaatccgtaa taaccttaca
     7681 gttaacctgt tgtcgcctgc tctggattaa cggataatag gcggcttttt tatttcaggc
     7741 cgaaaaatga ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta
     7801 ctggtcaagt ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg
     7861 atgggcatgg ggctggcaac aacgtttgtg atgacgctgg cgtctatttg cgcctggctt
     7921 atcgatacgt ggattttgat cccacttaat ctgatttacc tgcgcaccct ggcatttatt
     7981 ctggtgattg ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagcccggtg
     8041 ctttaccgtc tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc
     8101 gtggcgttgc tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt
     8161 tccgccgctg tcggtttctc gctggtgatg gtgctcttcg ccgccatccg cgaacgcctt
     8221 gctgtggctg atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt
     8281 cttatgtctc tggcctttat gggctttaat ggtttggtga agttgtaatg aatgctatct
     8341 ggattgccgt tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg
     8401 cctcccgccg ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac
     8461 cgcagagcca gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca
     8521 gctgtaacgg tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa
     8581 ttgccgagtt gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc
     8641 ctgcgcggat ggtggcggtt attgatgaaa ataactgtat tggctgcact aaatgtattc
     8701 aggcttgtcc ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg
     8761 atctctgtac gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc
     8821 aaccggtcgc agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta
     8881 tcattcccgt ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg
     8941 gatttcaacg gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacacccctg
     9001 cgccaggtac cactggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa
     9061 ggtgagttgt gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt
     9121 cgcggcaaaa tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc
     9181 cactctacgg ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt
     9241 gaagactgct ggatcccgcg cgacggctgg gccgattatc gcactcgcag tcgcgaagag
     9301 ttaatcgagc gcatacatca gtttggtgtt gccgggctgg gcggtgcagg attcccgaca
     9361 ggcgttaaat tgcagggtgg cggagataag attgaaacgt tgattatcaa cgcggctgag
     9421 tgcgagccgt acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta
     9481 gagggtattc gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa
     9541 gataacaaac cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt
     9601 tctctgcggg tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt
     9661 ctgaccggga agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa
     9721 aacgtcggca ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag
     9781 cgtgttgtaa ccctgactgg cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg
     9841 gggacgccag tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg
     9901 gtgattatgg gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta
     9961 aagattacca actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa
    10021 caaagctgca tccggtgtag cgcctgtgct gacgcctgcc ctgcggatct tttgccgcaa
    10081 cagttgtact ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgct
    10141 gattgcattg aatgtgggac ttgcgcgtgg gtttgcccga gcaatattcc cctggtgcaa
    10201 tatttccgtc aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca
    10261 gaagccaaag cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agcggctcgc
    10321 cttgaacgac ataagagcgc agccgttcaa cctgcagcca aagataaaga tgcgattgct
    10381 gccgctctgg cgcgggtgaa agagaaacag gcccaggcta cacagcctat tgtgattaaa
    10441 gcgggcgaac gcccggataa cagtgcaatt attgcagcac gggaagcccg taaagcgcaa
    10501 gccagagcga aacaggcaga actgcagcaa actaacgacg cagcaaccgt tgctgatcca
    10561 cgtaaaactg ccgttgaagc agctatcgcc cg
//
LOCUS       DAJTYZ010000136        10375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000136 DAJTYZ010000000
VERSION     DAJTYZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.136"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="OO596_003383"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="OO596_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6719162.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="OO596_003384"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="OO596_003384"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6719163.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="OO596_003385"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="OO596_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCR6719164.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="OO596_003386"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="OO596_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6719165.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="OO596_003387"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="OO596_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="OO596_003388"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="OO596_003388"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6719166.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="OO596_003389"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="OO596_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6719167.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OO596_003390"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OO596_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6719168.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="OO596_003391"
     CDS             complement(9457..10149)
                     /locus_tag="OO596_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6719169.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2530 a         2505 c         2743 g         2597 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcag
//
LOCUS       DAJTYZ010000137        10363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000137 DAJTYZ010000000
VERSION     DAJTYZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.137"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..154
                     /locus_tag="OO596_003392"
     CDS             <1..154
                     /locus_tag="OO596_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6719170.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="OO596_003393"
     CDS             258..1172
                     /locus_tag="OO596_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6719171.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="OO596_003394"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="OO596_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6719172.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="OO596_003395"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="OO596_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6719173.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="OO596_003396"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="OO596_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6719174.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="OO596_003397"
     CDS             5467..7149
                     /locus_tag="OO596_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6719175.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="OO596_003398"
     CDS             7201..8358
                     /locus_tag="OO596_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6719176.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="OO596_003399"
     CDS             complement(8324..8446)
                     /locus_tag="OO596_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719177.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8655..10340
                     /locus_tag="OO596_003400"
     CDS             8655..10340
                     /locus_tag="OO596_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR6719178.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         3028 a         2208 c         2214 g         2913 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     8521 agcataacca taaccataac cactgaaaat taaaaatttc tatatatttc atattgttaa
     8581 atgatactta aataaatttc atcagtaaat cattgccata gataatcatt ggatggcaca
     8641 atcaattttc gctaatgata accattccca ttacgtttta tatgttgata gcaaagtatc
     8701 tctgtctgct gaagcccttt tggctgcgta agaataataa aacttcagtg ttgttaatca
     8761 ttattatcct cgcaatgatc cttggcgtgg ttaaaattca ggtctggtta aacgactgga
     8821 ataatgattt tttcaatgcg ctaagccaga aagaaaccga taagctttgg cagcttgttc
     8881 tttggttccc tgccctgctg gggatctttg tattaatatc cgtaaacaaa acatggttaa
     8941 ttaaactact gactattcgc tggcgcgaat ggttaacaga ttactacctt aatcgctggt
     9001 ttgcagataa aaactactac ttcacgcaga tctacggcga gcataaaaat acagataacc
     9061 ctgaccaacg tatcgctgaa gacattctct tactgatcag caaaacgctc agtctttcat
     9121 ttggcttcat ccagtcactt agcatgctga tcacctttac cgttattctt tggcaaagcg
     9181 cgggtacgct ctcattcact gttggtggaa cagaatggaa tatccagggg tatatggtct
     9241 ataccgtcgt gctcattgtg atcggtggaa ctttatttac ccataaggtg ggtaaacgta
     9301 ttcgtccgct taatgtggaa aaacaacgta gcgaagcgac atttcgaact aatcttgtgc
     9361 agcataataa gcagacagaa ctgattgcgc taagcaatgc tgagtctcta caacgccagg
     9421 agttgcgcga caattttcat accattaaag agaactggca tcgtttaatg aatcgtcagc
     9481 ggtggcttga ttactggcaa aatatttatt cgcgctcgct tagcgttctt ccctactttc
     9541 tgttattgcc gcagtttatt agcgggcaga tcaatctggg cggactgatg aaatcgcgcc
     9601 aggcatttat gctagtatcg aacaatttaa gctggtttat ttataaatat gacgaacttg
     9661 ctgaactggc tgcggttatc gatcgcttgt atgagttcca tcaactcact gaacagcgcc
     9721 ctacgaataa gcctaaaaat tgccaacatg cggtacaagt ggctaatgcg agtattcgta
     9781 cgcctgataa taagatcata ttagagaact tgaactttca tgtttcgcca ggcaaatggc
     9841 tattactgaa aggctactct ggtgcgggaa aaaccacact gcttaaaaca ttatcccact
     9901 gctggccgtg gtttaaaggt gatatttctt ctcctgctga cagttggtat gtgtcacaaa
     9961 caccgttaat caaaagcggc ttactgaaag agattatttg taaagcactt cccctgcccg
    10021 tagacgataa atcgttgagc gaagtactgc atcaggttgg tctggggaaa ttggctgcgc
    10081 gtattcatga ccacgatcgc tggggagata ttctttccag cggcgaaaaa caacgtatcg
    10141 ccctggcacg attaatttta cgacgtccga aatggatatt tcttgacgaa actacctctc
    10201 atcttgagga acaagaggct atacgcttac tgcgtttagt gcgtgaaaaa ctacccacaa
    10261 gcggcgtcat tatggttaca catcaacccg gtgtctggaa cctggctgat gatatttgtg
    10321 acattagcac ggttttataa caacaataaa agccctgctg tac
//
LOCUS       DAJTYZ010000138        10332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000138 DAJTYZ010000000
VERSION     DAJTYZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.138"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(43..1047)
                     /gene="cra"
                     /locus_tag="OO596_003401"
     CDS             complement(43..1047)
                     /gene="cra"
                     /locus_tag="OO596_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6719179.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1129..1215)
                     /locus_tag="OO596_003402"
     CDS             complement(1129..1215)
                     /locus_tag="OO596_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6719180.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1227..1718)
                     /gene="ilvN"
                     /locus_tag="OO596_003403"
     CDS             complement(1227..1718)
                     /gene="ilvN"
                     /locus_tag="OO596_003403"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6719181.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1721..3445)
                     /gene="ilvI"
                     /locus_tag="OO596_003404"
     CDS             complement(1721..3445)
                     /gene="ilvI"
                     /locus_tag="OO596_003404"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6719182.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQAHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3564..3608
                     /locus_tag="OO596_003405"
     CDS             3564..3608
                     /locus_tag="OO596_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719183.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3763..4707)
                     /gene="leuO"
                     /locus_tag="OO596_003406"
     CDS             complement(3763..4707)
                     /gene="leuO"
                     /locus_tag="OO596_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6719184.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5367..5453
                     /gene="leuL"
                     /locus_tag="OO596_003407"
     CDS             5367..5453
                     /gene="leuL"
                     /locus_tag="OO596_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6719185.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5546..7117
                     /gene="leuA"
                     /locus_tag="OO596_003408"
     CDS             5546..7117
                     /gene="leuA"
                     /locus_tag="OO596_003408"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6719186.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7117..8208
                     /gene="leuB"
                     /locus_tag="OO596_003409"
     CDS             7117..8208
                     /gene="leuB"
                     /locus_tag="OO596_003409"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6719187.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8211..9611
                     /gene="leuC"
                     /locus_tag="OO596_003410"
     CDS             8211..9611
                     /gene="leuC"
                     /locus_tag="OO596_003410"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6719188.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9622..10227
                     /gene="leuD"
                     /locus_tag="OO596_003411"
     CDS             9622..10227
                     /gene="leuD"
                     /locus_tag="OO596_003411"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6719189.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2707 a         2715 c         2523 g         2387 t
ORIGIN      
        1 cgccagaggg aaattcacct ggcgcgtatt tttgttcgcg gcttagctac ggctgagcac
       61 accgcggcga tagagattac gtttaatgcg cgttaaacca ggttttggct tacgcggttc
      121 gtccaggctt gccaggacaa tctccagcac acgctctgcg acatcgcggt gacgttgagc
      181 cactgccagc accgggcact gtaagaagtc gagcagttcg ttatcgccaa aggtggcaat
      241 tgccaggtca gaaggcagtt taccatcgcg acgtagcgtg acatccatca ccccttgcaa
      301 caacgcaaac gacgttgtga acagcgcctg cggcatcgga tgcgtttcca gccatttttc
      361 gaataactgg gcagccacct cccgctcata gctgttggca tacaggaaat gcacttcgcg
      421 cggatcatct ttccaggcag tacggaaacc ttgttcacgc aggaagctga cagaaagctc
      481 cggcagcgcg ccaagataaa gcaccgtctc ggcgggaaac ttacgtaact cttccgccag
      541 catttcggca tcatcctgat cggcaccaac cacgctggtg aagtgttcac gatcgagggc
      601 gcggtccagc gcgacaatcg ggaacgggtc gttagcccag cgttgataaa aaggatgctc
      661 aggaggcaac gacgtcgaaa caataatggc atcgacctga cgctgtaaaa ggtgctcaat
      721 acagcgcatt tcgttgtctg gctgatcttc tgagcaggca attagcagtt gataaccccg
      781 ttgccgcgcc tggcgttcaa gatagttagc gatgcgggta tagctggtgt tctccagatc
      841 ggggatcaca agaccaatag aacgtgtgcg tccagcacga agcccagctg ccacggcgtt
      901 cgggtggtaa ttgtgctcac gcaccacagc catgactttt tcaacggttt tgtcgctcac
      961 acggtattgc ttcgctttgc cgttaataac atagcttgca gtggtccgcg acactcccgc
     1021 cagccgagcg atttcatcca gtttcacaat tgccccttgc gtaaaatgta aaaaccatag
     1081 ccattgtact ggtatgggtt aaattcatta acatctaagc gcataaaatt aactgcggca
     1141 actgctttta ttccaggttc ccactgattt cgcaaaaaaa gcccaacgtg acatgttggg
     1201 ctgcaaattg cgcattcaga tcattctcaa cgcattattt tatcgccgcg cgaaagtccg
     1261 accacaccag aacgagcaac ttccacaatt ttcgccacct cgcgaatcga tgctaaaaat
     1321 gcatcaagct tatcgctggt gcctgctaat tgaacggtat aaagcgaggg tgtgacatcg
     1381 ataatttgcc cacggaatat ttccgtatta cgtttcactt cgtcacgccc gtaaccgctg
     1441 gcctgaattt tcaccagcat gatttcccgc tcaacatgcg cgccctgccc caactcactc
     1501 acgcgcaaga catcgaccag cttgtgtaat tgcttttcga tctgctcaag tactttttca
     1561 tcgcccacgg tctggatggt catacgcgat aatgtcggat catcggttga cgcaacggtc
     1621 aggctttcaa tgttgtagcc acgctgggaa aaaaggccaa tcacgcggga taacgcgcct
     1681 gattcatttt cgagtaagac tgataatatc cggcgcataa tcaggttctc tccgttttgc
     1741 ttaaccacat ttcatccatt ccgcccccgc gaatctgcat cgggtagacg tgctcgctgc
     1801 catcgacggt aacatcaaca aacaccaggc gattattgtg cgcctgttcc agcgcctcgc
     1861 taagtttgct ttccagctca tgcggatgag aaatctggat cccgacgtga ccataggctt
     1921 ccgccagacg gacgaaatcg ggtagcgatt gcatataaga ttgtgaatga cggccggaat
     1981 agatcatgtc ctgccactgc ttcaccatcc ccagatagcg gttattgaga ttcaccacca
     2041 gtacgggcaa ctcgtattgc aacgcggtag acagttcctg gatgttcatc tgaatactgc
     2101 cgtcgccagt gacgcaaacc acggtttctt ctggcaacgc cattttgacg cccagtgccg
     2161 caggtaaacc aaaacccatc gtgccgaggc caccggaatt gatccagcga cgcggtttgt
     2221 cgaatggata ataaagtgca gcaaacatct ggtgctgccc gacatcggac gtcacgtaag
     2281 cgtctccctt cgtcaaccgc caaagagtct cgatcaccgc ctgcggttta atcttttcac
     2341 tgtgagtgtc atatttcagg cactgacgag cgcgccactg ttcaatttgc tgccaccagt
     2401 cgcggatctc atccagtggt tgatgggcgg attcttgcga caagagttca agcatttgtt
     2461 cgaggacctg gcgagcatcc cccacaatcg ggatatccgc agtcacggtt ttagaaatgg
     2521 aagtaggatc aatatcgata tgcagaacag tggcatttgg gcagtacttt gccagattgt
     2581 tcgtcgttcg gtcatcaaat cgtaccccga cggcgaaaat cacatccgcg ttatgcatcg
     2641 tcatattggc ttcgtaggta ccgtgcattc ccagcatgcc cagtgcctga cgatgcgttg
     2701 ccggaaacgc tcccagcccc atcaatgagg aaacaacggg cagattcaac gcctccaccg
     2761 tttctttcaa ctgctgatgg cagcccgccg tgattgcccc accgcctacg tagacaaccg
     2821 gtttttttgc cgctaccagc gtttgcagag cgcgcttaat ttgcccttta tgtccggtag
     2881 tagtgggatt gtaagaacgc atactgaccg actccggcca gacatagggt aatttgttcg
     2941 ccggattaag aatatctttc ggtaaatcaa cgactactgg tccaggacga ccacttgccg
     3001 ccagccagaa agcctttttc agcacctgcg gaatgtcttc cgtttgctta accagaaaac
     3061 tgtgtttaac cactggtcgc gaaatcccca ccatgtcgca ctcctgaaag gcatcgtaac
     3121 ctatcaacga ggtcgctacc tgcccggaaa ggacaactaa tggaatggaa tccatataag
     3181 cggtggcgat gccagtaatc gcattggtcg cccctggacc cgacgttacc agcacgacgc
     3241 cgacttcccc ggtcgcgcgc gccagaccat cggccatatg cactgccgcc tgctcatgac
     3301 gaactaaaac atgatcgata ccacccacgg tatgcaatgc atcgtaaata tcaaggactg
     3361 cgcctccggg ataaccgaat acttgtttaa cgccctgatc gataagcgat cggacgacca
     3421 tctcggctcc agacaacatc tccatggcct gcctcactgt ttgacggaaa aaatgtgtaa
     3481 aacacattaa ccgtccgatc ttgcttaagc aattggcagc cagagacata aacgcagggt
     3541 aataagaata aacaggagga aaggtgaaaa gaaacgataa aatcctccat tacgaaggat
     3601 taaattgata ataaatcggc tgaatcccac aacttacagc aatttctcta tttttttaat
     3661 taatcctaaa atagagagcc cacacattca gaataaaatt ctacattcaa taaaaaatac
     3721 agaataaacc agacattcat gtctgaccta ttctgcattc agttagcgtt tgcaaattga
     3781 gactaattgc tcttccatcc actgatggcc tttatcgcgc ccggcagctt catgccagga
     3841 gagataacag gttctgctat tttgttttaa cggcagcggt aatacctgta attctaagga
     3901 ttcagcgaac tcttcagcca gccaacgcgg cgcaatagcg accaaatgcg tttgcgacac
     3961 cacgctaagt acgctcatca ttgccatgcc ctgatacgcg atactggctt gcttatctac
     4021 cgtgtcatac caaggttgac taaatgacgc gaaacgatcg agcgaaaccg ccgcatgttg
     4081 ttcgttataa acatcatgtt tcagtaacgg gcccttaatt gttggatgat ttttgctggc
     4141 taccagcacc atttcatctt taaataatgg cacgctggta aattcaggac gatggaagtc
     4201 ttcataacta atcacaaact ccgtttcctg ataacgcagc tgatgttcag tgttctgatt
     4261 taatgaagac ttgaacataa catgtatatt tggcgcaatc tgctcaatgt gattataaat
     4321 ctgcgaggtc agaatgctgt ctaacgggct gcaaacacaa agatgaaata cacgttcact
     4381 gctcgcgggt tcaaaacctg aaccaggcaa ttcattttgt actagttgca atgcctgacg
     4441 aactgagcca aaaagttgaa atgcgcgagc agtcggttga ataccacggc cataacgaac
     4501 aaaaagctcg tcattaaaca tcaccttcag gcgtgcaaca gcgttactga ccgcaggttg
     4561 cgacataccc agaacatgag cggcacgggt aatgttttgc tcctgcatca cggcatcgaa
     4621 aacggttaat aagttgagat cgaccatgcg tagctgtggc ttgcttaact ccgccgtctc
     4681 tgaatgatct gtttgtacct ctggcatact taactccact gtcacactta actccctttc
     4741 ccttattgga atgcagagaa ataatcctga agaatatgat ttatcatgca tataaaataa
     4801 gaaaaagcaa aatgagtaaa attcggaaaa cataaagata cgggttctga taaaaacaga
     4861 atccattgcc gtggaatgag tcatttacca cagcataaga atccataatg tttttgttgc
     4921 gaaaacaatc taatcataac taccgcgaat actcaatcat ctacaaaatg gattaaatgt
     4981 gcttatttaa taattaattt atatatttaa tgcattaatt cttaacatta attgatcaat
     5041 aatattcacc aaatcaatat caaaaaaaat cgcaaaacat ataattcaat acaaatcatc
     5101 aggataggtt ttgcaacgcg tgcattttgt cccctttttc ctcgttgatt agatgcaaaa
     5161 atttatgctg aaatatgtca accgatgaaa agcgtcggta gttaagcaga aattaatatc
     5221 gcttacttta accaccgcag cacaattagc taattttacg gatgcagaac tcacgctggc
     5281 gggacgtttt tattgcgtca gggttgacat ccgtttttgt atccagtaac tctaaaagca
     5341 tatcgcattc atctggagct gatttaatga ctcacatcgt tcgctttatc ggtctactac
     5401 tactaaacgc atcttctttg cgcggtagac gagtgagcgg catccagcat taagccagca
     5461 cgcagtcaaa caaaaaaccc gcgccattgc gcgggttttt ttatgcccga agcgaggcgc
     5521 tctaaaagag acaaggacct aaaccatgag ccagcaagtc attattttcg ataccacatt
     5581 gcgcgacggt gaacaggcgt tacaggcaag cttgagtgtg aaagaaaaac tgcaaattgc
     5641 gctggccctt gagcgtatgg gtgttgacgt gatggaagtc ggtttccccg tctcttcgcc
     5701 gggcgatttt gaatcggtgc aaaccatcgc ccgccaggtt aaaaacagcc gcgtatgtgc
     5761 gttagctcgc tgcgtggaaa aagatatcga cgtggcggcc gaatccctga aagtcgccga
     5821 agccttccgt attcatacct ttattgctac ctccccaatg cacatcgcca ccaagctgcg
     5881 cagcaccctg gacgaggtga tcgaacgcgc tatctatatg gtgaaacgcg cccgtaatta
     5941 caccgatgat gttgaatttt cttgcgaaga tgccgggcgt acacccattg ccgatctggc
     6001 gcgagtggtc gaagcggcga ttaatgccgg tgccaccacc atcaacattc cggacaccgt
     6061 gggctacacc atgccgtttg agttcgccgg aatcatcagc ggcctgtatg aacgcgtacc
     6121 taacatcgac aaagccatta tttccgtaca tacccacgac gatttaggcc tggcggtcgg
     6181 caactcactg gcggcggtac atgccggtgc acgccaggtg gaaggtgcaa tgaacgggat
     6241 cggcgagcgt gccggtaact gttcgctgga agaagtcatc atggcgatta aagttcgtaa
     6301 ggatattctc aacgtccaca ccgccattaa tcaccaggag atatggcgca ccagccagtt
     6361 agttagccag atttgtaata tgccgatccc ggcaaacaaa gccattgttg gcagcagcgc
     6421 attcgctcac tcctccggta tccaccagga tggcgtgctg aaaaaccgcg aaaactacga
     6481 aatcatgaca ccagaatcta ttggtctgaa ccaaatccag ctgaatctga cctctcgttc
     6541 ggggcgtgcg gcggtgaaac atcgcatgga tgagatgggg tataaagaaa gtgaatataa
     6601 tttagacaat ttgtacgatg ctttcctgaa gctggcggac aaaaaaggtc aggtgtttga
     6661 ttacgatctg gaggcgctgg ccttcatcgg taagcagcaa gaagagccgg agcatttccg
     6721 tctggattac ttcagcgtgc agtctggctc taacgatatc gccaccgccg ccgtcaaact
     6781 ggcctgtggc gaagaagtca aagcagaagc cgccaacggt aacggtccgg tcgatgccgt
     6841 ctatcaggca attaaccgca tcactgacta taacgtcgaa ctggtgaaat acagcctgac
     6901 cgccaaaggt cacggtaaag atgcgctggg tcaggtggat attgtcgcca actacaacgg
     6961 tcgccgcttc cacggcgtcg gcctggccac cgatattgtc gagtcctccg ccaaagccat
     7021 ggtgcacgta ctgaacaata tctggcgtgc cgcagaagtc gaaaaagagt tgcaacgcaa
     7081 agctcaacac aacgaaaaca acaaggaaac cgtgtgatgt cgaagaatta ccatattgcc
     7141 gtattgccgg gggacggtat tggtccggaa gtgatgaccc aggcgctgaa agtgctgaat
     7201 gccgtgcgca accgctttgc gatgcgcatc accaccagcc attacgatgt aggcggcgca
     7261 gccattgata accacgggca accactgccg cctgcgacgg ttgaaggttg tgagcaagcc
     7321 gatgccgtgc tgtttggctc ggtaggcggc ccgaagtggg aacatttacc accagaccag
     7381 caaccagaac gcggcgcgct gctgcctctg cgtaagcgct tcaaattatt cagcaacctg
     7441 cgcccggcaa aactgtatca ggggctggaa gcattctgtc cgctgcgtgc agacattgcc
     7501 gctaacggct tcgacatcct gtgcgtgcgc gaactgaccg gcggcatcta tttcggtcag
     7561 ccaaaaggcc gcgaaggtag cggacaatat gaaaaagcgt ttgataccga ggtgtatcac
     7621 cgttttgaga tcgagcgtat tgcccgcatc gcgtttgaat ctgctcgtaa acgtcgccac
     7681 aaagtcacct caatcgacaa agctaacgtg ctgcaatcct ctattttatg gcgtgaaatc
     7741 gtcaacgaga tcgccacgga atacccggat gtcgaactgg cgcatatgta catcgacaac
     7801 gccaccatgc agctgattaa agatccatca cagtttgacg ttctgctgtg ctccaacctg
     7861 tttggcgaca ttctgtctga cgagtgcgca atgatcactg gctcgatggg gatgttgcct
     7921 tccgccagcc tgaacgagca aggttttggt ctgtatgaac cggcaggcgg ctcagcacca
     7981 gatatcgcag gcaaaaacat cgccaacccg attgcgcaaa ttctgtcgct ggcactgctg
     8041 ctgcgctaca gcctggatgc cgatgatgcg gcttgcgcca ttgaacgcgc cattaaccgc
     8101 gcattagaag aaggcattcg caccggggat ttagcccgtg gcgctgccgc cgttagtacc
     8161 gatgaaatgg gcgatatcat tgcccgctat gtggcagaag gggtgtaatc atggctaaaa
     8221 cgttatacga aaaattattc gacgctcacg tagtgtacga agccgaaaac gaaaccccgc
     8281 tgttatatat cgaccgccac ctggtgcatg aagtgacctc accgcaggcg ttcaatggtc
     8341 tgcgcgccca cggtcgcccg gtacgtcagc cgggcaaaac cttcgccact atggatcaca
     8401 acgtctctac ccagacgaaa gacattaatg cctgtggtga aatggcgcgc atccagatgc
     8461 aggagctgat taaaaactgc aaagaatttg gcgtcgaact gtatgacctg aatcacccgt
     8521 atcaggggat cgtccacgta atggggccgg aacagggcgt caccttgccg gggatgacca
     8581 ttgtctgcgg cgactcgcat accgccaccc acggcgcgtt tggcgcactg gcctttggta
     8641 tcggcacttc cgaagttgaa cacgtactgg caacgcaaac cctgaaacag ggccgcgcaa
     8701 aaaccatgaa aattgaagtc cagggcaaag ccgcgccggg cattaccgca aaagatatcg
     8761 tgctggcaat tatcggtaaa accggtagcg caggcggcac cgggcatgtg gtggagtttt
     8821 gcggcgaagc aatccgtgat ttaagcatgg aaggtcgtat gaccctgtgc aatatggcaa
     8881 tcgaaatggg cgcaaaaacc ggtctggttg caccggacga aaccaccttt aactatgtca
     8941 aaggccgttt gcatgcgccg aaaggcaaag atttcgacga cgccgttgcc tactggaaaa
     9001 ccctgcaaac cgacgaaggc gcaactttcg ataccgttgt cactctgcaa gcagaagaaa
     9061 tttcaccgca ggtcacctgg ggcaccaatc caggccaggt gatttccgtg aacgacaata
     9121 ttcccgatcc ggcttcgttt gccgatccgg ttaaacgcgc gtcggcagaa aaagcgctgg
     9181 cctatatggg gctgaaaccg ggcattccgc tgaccgaagt ggctatcgac aaagtgttta
     9241 tcggttcctg taccaactcg cgcattgaag atttacgcgc ggcagcggag atcgccaaag
     9301 ggcgaaaagt cgcgccaggc gtgcaggcac tggtggttcc cggctctggc ccggtaaaag
     9361 cccaggcgga agcggaaggt ctggataaaa tctttattga agccggtttt gaatggcgct
     9421 tgcctggctg ctcaatgtgt ctggcgatga acaacgaccg tctgaatccg ggcgaacgtt
     9481 gtgcatccac cagcaaccgt aactttgaag gccgccaggg gcgcggcggg cgcacgcatc
     9541 tggtcagccc ggcaatggct gccgctgctg ctgtgaccgg acatttcgcc gacattcgca
     9601 acattaaata aggagcacac catggcagag aaatttatca aacacacagg cctggtggtt
     9661 ccgctggatg ccgccaatgt cgataccgat gcaatcatcc cgaaacagtt tttgcagaaa
     9721 gtgacccgta cgggttttgg cgcgcatctg tttaacgact ggcgttttct cgatgaaaaa
     9781 ggccaacagc caaacccgga cttcgtgctg aacttcccgc agtatcaggg cgcttccatt
     9841 ttgctggcac gagaaaactt cggctgtggc tcctcgcgtg agcacgcgcc ctgggcattg
     9901 accgactacg gctttaaagt ggtgattgcg ccgagttttg ctgacatctt ctacggcaat
     9961 agctttaaca accagctgct gccggtgaaa ttaagcgatg cagaagtgga cgaactgttt
    10021 gcgctggtga aagccaatcc ggggatccat ttcgacgtgg atctggaagc gcaagaggtg
    10081 aaagcgggag agaaaaccta tcgctttacc atcgatgcct tccgccgcca ctgcatgatg
    10141 aacggtctgg acagtattgg gcttaccttg cagcacgacg acgccattgc cgcttatgaa
    10201 gcaaaacaac ctgcgtttat gaattaatcc ccttgcccgg tcaaatgacc gggctttccg
    10261 ctatcgtcca cgtcatcaaa cgtctttaac ctttgcggtt aaaaataatg cgacaacaga
    10321 aataaccgca at
//
LOCUS       DAJTYZ010000139         9986 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000139 DAJTYZ010000000
VERSION     DAJTYZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.139"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..611
                     /locus_tag="OO596_003412"
                     /pseudo
     CDS             1..611
                     /locus_tag="OO596_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /locus_tag="OO596_003413"
     CDS             608..1222
                     /locus_tag="OO596_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR6719190.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OO596_003414"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OO596_003414"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6719191.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="OO596_003415"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="OO596_003415"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6719192.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="OO596_003416"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="OO596_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6719193.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="OO596_003417"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="OO596_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6719194.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6338)
                     /gene="phoE"
                     /locus_tag="OO596_003418"
     CDS             complement(5289..6338)
                     /gene="phoE"
                     /locus_tag="OO596_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6719195.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVMHYMSD
                     NASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKY
                     KDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGV
                     IDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQ
                     SRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDF
                     GLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLN
                     INNDDIVAVGMTYQF"
     gene            6626..7729
                     /gene="proB"
                     /locus_tag="OO596_003419"
     CDS             6626..7729
                     /gene="proB"
                     /locus_tag="OO596_003419"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6719196.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7741..8994
                     /gene="proA"
                     /locus_tag="OO596_003420"
     CDS             7741..8994
                     /gene="proA"
                     /locus_tag="OO596_003420"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6719197.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9109..9184
                     /locus_tag="OO596_003421"
     tRNA            9109..9184
                     /locus_tag="OO596_003421"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9142..9144,aa:Thr,seq:cgt)
     gene            9790..9948
                     /locus_tag="OO596_003422"
     CDS             9790..9948
                     /locus_tag="OO596_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719198.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2472 a         2437 c         2598 g         2479 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcataacttt gccatagaca
     6241 tccagtttat taccgtcttt attatatatt tctgcagcct gtacagatgc agatgccaca
     6301 atgcccatca ccactaatgc cagagtgctc tttttcattt tcattcctga ttttaattaa
     6361 cgcgcgaata ttcagcggga gagccccgtt gaaaacagga aagtttttaa cctgagattg
     6421 ttaaagatat attacagatt aataatattc ttaaaatgtg gtaatttatt aaatctgtaa
     6481 taaaagcgta aacaactgcc gctaggcttg ctgatcccgc gcaacaaaac gccatgcttt
     6541 gctcgcagat ggttggcaac cgacgacagt cctgctaaaa cgttcgtttg atatcatttt
     6601 tcctaaaatt gaatggcaga gaatcatgag tgacagccag acgctggtgg taaaactcgg
     6661 caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt gcccatattg ttgaacttgt
     6721 tcgccagtgc gcgcagttac atgccgccgg gcatcggatt gttattgtga cgtcgggcgc
     6781 gatcgccgcc ggacgtgagc acctgggtta cccggaactg ccagcgacta tcgcctcgaa
     6841 acaactgctg gcagcggtag ggcagagtcg actgattcaa ctgtgggaac agctgttttc
     6901 gatttatggc attcacgtcg ggcaaatgct gctgacccgt gctgatatgg aagaccgtga
     6961 acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc gataacaata tcgttccggt
     7021 aatcaatgag aacgatgctg tcgctacggc agagattaaa gtcggcgata acgataacct
     7081 ttctgcgctg gcggcgattc tggcgggtgc cgataaactg ttgctgctga ccgatcaaaa
     7141 aggtttgtat accgctgacc cgcgcagcaa tccgcaggca gaactgatta aagatgttta
     7201 cggcattgat gacgcactgc gcgcgattgc cggtgacagc gtttcaggcc tcggaactgg
     7261 cggcatgagt accaaattgc aggccgctga cgtggcttgc cgtgcgggta tcgacaccat
     7321 tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg atggaaggca tttccgtcgg
     7381 tacgctgttc catgcccagg cgactccgct tgaaaaccgt aaacgctgga ttttcggtgc
     7441 gccgccggcg ggtgaaatca cggtagatga aggggcaact gccgccattc tggaacgcgg
     7501 cagctccctg ttgccgaaag gcattaaaag cgtgactggc aatttctcgc gtggtgaagt
     7561 catccgcatt tgcaacctcg aaggccgcga tatcgcccac ggcgtcagtc gttacaacag
     7621 cgatgcatta cgccgtattg ccggacacca ctcgcaagaa attgatgcaa tactgggata
     7681 tgaatacggc ccggttgccg ttcaccgtga tgacatgatt acccgttaag gagctggccg
     7741 atgctggaac aaatgggcat tgctgcgaag caagcctcgt ataaattagc gcaactctcc
     7801 agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc acaaagcgaa
     7861 atcatcctca acgctaacgc ccaggatgtt gctgacgcgc gagccaatgg ccttagcgaa
     7921 gcgatgcttg accgtctggc actgacgccc gcacggctga aaggcattgc cgacgatgta
     7981 cgtcaggtgt gcaacctcgc cgatccggtg gggcaggtaa tcgatggcgg cgtactggac
     8041 agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg ttattggcgt gatttatgaa
     8101 gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg taatgcggtg
     8161 atcctgcgcg gtggcaaaga aacgtgtcgc actaacgctg caacggtggc ggtgattcag
     8221 gacgccctga aatcctgcgg cttaccggcg ggtgccgtgc aggcgattga taatcctgac
     8281 cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca tcgacatgct gatcccgcgt
     8341 ggtggcgctg gtttgcataa actgtgccgt gaacagtcga caatcccggt gatcacaggt
     8401 ggtataggcg tatgccatat ttacgttgat gaaagtgtag agatcgctga agcattaaaa
     8461 gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta atacggttga aacgttgctg
     8521 gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat ggcggaaagc
     8581 ggcgtgacat tacacgcaga tgcagctgca ctggcgcagt tgcaggcagg ccctgcgaag
     8641 gtggttgctg ttaaagccga agagtatgac gatgagtttc tgtcattaga tttgaacgtc
     8701 aaaatcgtca gcgatcttga cgatgccatc gcccatattc gtgaacacgg cacacaacac
     8761 tccgatgcga tcctgacccg cgatatgcgc aacgcccagc gttttgttaa cgaagtggat
     8821 tcgtccgctg tttacgttaa cgcctctacg cgttttaccg acggcggcca gtttggtctg
     8881 ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat ggggctggaa
     8941 gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc gtaaataaaa
     9001 ccgggtgatg caaaagtagc catttgattc ataaggccat tgacgcatcg cccggttagt
     9061 tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc ctttcagggc cgatatagct
     9121 cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag gttcgactcc tattatcggc
     9181 accattaaga aaattaataa cttagcttaa cttctctaag aaattgtatc ttctgttatc
     9241 acttacaaac gcctatacgt tgttatgggc tttggtagtg taacacgtat gggcgagtag
     9301 ttcccaggct caacctcgta acaagttcct ttgaagctgg aagacgtgcc accgtctcta
     9361 aaccttctac caaaactctt agcaagatgt tccacacctg gcgcggtgtc caatgatgct
     9421 acacgtcgtg agacgttgca gaggataaac aggctaaatg atgggaagag ttttaagtgc
     9481 aatagtggat taaagcccac ctcttcaaaa cctaacttga tcgggaaaga atcaagctct
     9541 agcacggttt cgtccaccgg agtacaacgg gtgcctgata agagaagaac tgctaagact
     9601 tcatagcttt tgtgaaaaag ccctttttag tggatacgcc tacggcgctt attttttggt
     9661 ataaccatca aaggcgcttt atggctccta ttgtcttgaa aatgtatctt tacttgtatt
     9721 gtgttttatg cgtagtctcc ctgtgtagta tgtaacttgt taattttcat atgaatacag
     9781 aggcacacga tgaaaaacat tgcagcaatc aaacgtaata accgcaagat tcacgctcgt
     9841 aagttcctgt ctacgccaga aggaaaagcc tggctagagc gtaaacagag agagaacgaa
     9901 gaaagaaaac tccttagtga gttgaaatgg cttagagagg atttctgaaa atctccagaa
     9961 cgcaacagga cgcattctaa cgctgt
//
LOCUS       DAJTYZ010000140         9820 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000140 DAJTYZ010000000
VERSION     DAJTYZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.140"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OO596_003423"
     CDS             complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OO596_003423"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6719199.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1374..1787
                     /gene="msrB"
                     /locus_tag="OO596_003424"
     CDS             1374..1787
                     /gene="msrB"
                     /locus_tag="OO596_003424"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6719200.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1829..2101
                     /locus_tag="OO596_003425"
     CDS             1829..2101
                     /locus_tag="OO596_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6719201.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OO596_003426"
     CDS             complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OO596_003426"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6719202.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OO596_003427"
     CDS             complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OO596_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6719203.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIMTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OO596_003428"
     CDS             complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OO596_003428"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6719204.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6114..6665
                     /locus_tag="OO596_003429"
     CDS             6114..6665
                     /locus_tag="OO596_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6719205.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6782..7825
                     /gene="selD"
                     /locus_tag="OO596_003430"
     CDS             6782..7825
                     /gene="selD"
                     /locus_tag="OO596_003430"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6719206.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7830..9791
                     /gene="topB"
                     /locus_tag="OO596_003431"
     CDS             7830..9791
                     /gene="topB"
                     /locus_tag="OO596_003431"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6719207.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2434 a         2450 c         2617 g         2319 t
ORIGIN      
        1 gaagtcgctc tttttagatc acagtttcat ctcaacttat ttggagatgt gagcgatcag
       61 gtccagaact ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa
      121 gttgtcgttc agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt
      181 gaaatcggta gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc
      241 agcagcggct ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac
      301 ggtcaggtca actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt
      361 gccattcagt tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat
      421 gatgttctgg gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt
      481 tttctgagta gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt
      541 atcgttgata actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac
      601 gatgtcctgg ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc
      661 tttagacgga ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt
      721 ttcgtcagtc aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca
      781 tttcaggtta gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag
      841 atgaccgtct ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag
      901 catgtatgcc atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga
      961 acgtttctga gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac
     1021 tttgatagtc atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt
     1081 acaaaaacct tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg
     1141 tgcctaagca ttacgcgact gactcgcctc actcttcctt tgggcttgaa accacatggg
     1201 gtcggcgccc cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta
     1261 ggtgcagcca tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt
     1321 tttgttagaa tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta
     1381 ataaaccttc ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc
     1441 agaatcatgg gacagagccg ccatttacgg gtcgtttact gcataacaag cgtgacggcg
     1501 tatatcactg tttgatctgc gatgccccgc tgtttcattc ccaaaccaag tatgattccg
     1561 gctgtggttg gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag
     1621 acttgtcaca tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg
     1681 ggcatgtctt ccctgacggg ccgcagccaa cgggcgaacg ttattgtgtt aactctgctt
     1741 ctttacgctt cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag
     1801 caacttattc cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac
     1861 gcctgaggta taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ccgatggcgt
     1921 tgcgttgacc gaggaacaaa aagcgaactg cctgcaactg gtgatgctgt ggcaagctcg
     1981 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
     2041 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctattgcta tgtttaagtg
     2101 aatgagttat ctagtattta agcctgttat taagggaggg caaatatact tgttaggacg
     2161 tcgtgtttta gctgcaagta ggttaaatgc aacactgaca agtattatct ggcctacaat
     2221 gtcattgagg aaatgtaggc cagagatgaa ttcaactaat attacccctg tgtctcttcc
     2281 cagtctgcca gcgtatatag cgttgcccca gctgctgaca tctccataaa cgcgtgcgca
     2341 ctgtcctggg gctggatatt cacgccacga caaccatcgg taatcacatt taccttataa
     2401 cctaactgta acgcgtccag cacggtaaac ttcacgcaat agtcagtagc caggcccata
     2461 acgatcaatt catcgatttc atgatcgcgt aaccagtcat cgagcgaggt tttctgccga
     2521 cggccgttat caaaaaaggc actgtaactg tcaactaaag gattttcgcc tttatggaac
     2581 accgctgcga tcgctttttg gttcagtaac ggatgtaatt gtgcgccttc actgttctgc
     2641 acacagtgat ctggccagaa ggtttgtggc aaaccgtcga gttggcctgg cgtataaggc
     2701 tctacaccgt gctgactggc aaaactgccg tgattcgccg ggtgccagtc ctgactggcg
     2761 ataaccgctt caccgcgcga ctggcaccag tcaatcaggc ggttagcgac atccaccgta
     2821 ctgtcacctt ccggcacggc gagcgcgcca ccagcacaga aatcattttg taaatcgacc
     2881 agtaacaggg cgcgaggggg cattacagtc tccttaatca tccggcgtca gttcgccgcg
     2941 caggttttgg ctcatggcct tgcgaatggt ttcagtatcc agttcctggc tcagcaggta
     3001 atgcagtttg gttagcgtgg cttctacagt catatctgca ccgccaataa cgccggcatg
     3061 ggcgagggcg ttaccggtgg cataaccacc catattcact ttaccggaca tacattgtgt
     3121 caggttgacc accacaatac cgcgatcgct ggcttcttgt aattcctgca ggaaggcttt
     3181 gttttgtggc gcattaccca cgccatagga gcgcagaatc aatgctttca ccggttggcg
     3241 cagaaaattg cgcacgacgt cagcagaaat ccctggataa atcgtcacta cgccaattgg
     3301 ttgtggcgtg attggatgaa cgattaattc cccttcaccg tgcggggcgg gtggcgtatt
     3361 caaacgacga atatggatac cagcttccag taacggagga aggtttggag aggcaaacgc
     3421 atcaaaacca tcggcatggg ctttggtagt gcggttgccg cgatacaatc ggttattgaa
     3481 aaagagcgtt acttcgttga tcggataatt cgccgcaacg tacaacgcat tcagcagatt
     3541 aatttgtccg tcagagcgta actcagccag cgggatttgt gaccctgtca taatgaccgg
     3601 tttaccgaga ttctcgagca tgaacgacag cgcagaggca gtatacgcca tcgtgtcggt
     3661 gccgtgcagg atgacaaaac catcatagtc gtcatagtgc gctttaatat cttcagcaat
     3721 atgctgccag tcttctggcg tcatatcaga agaatccatc agcggcgtat attcatgaat
     3781 ggtgaaatct ggcatctccg ggcgatggaa ttccggcatc agcgccagtt ggcgttgtag
     3841 atgacctgac accggaatat aaccctgctc ggaacgctgc atcccgatgg tcccgcccgt
     3901 gtaggcgacg taaattgatt tcttttgcat gatattgata tgaatgatat cgaaagagca
     3961 aaagtatata cgtgaggcag gaggagcgca cctgatacta tgcggataag gaaatatggg
     4021 gcgagacagc gggaagagga ttcgtcgacg taaaaaataa tgtggccgac gatctgtcgg
     4081 ccactcagta caagacttaa cgcacgttgg cgcaggtcag gcaaaacgca taacggtttt
     4141 gtgggtcgtt aaacgcggcc agcttgtcgc tttcactttt aacggtagag gctaccgagg
     4201 caagcggtgc aggtaacatg gcctggaacg catctggcaa cattgcccgg acagaaccag
     4261 acatgttgtc catcactttg tcgaagaagg tcggttcatc aacgtagtat tccagatgcc
     4321 actgtttcac ttttgccagc tctgctgctt tggcgaccgc atcatcgaaa tccccgagac
     4381 tatcgaccag cccgttagct tttgcatcct gaccggtcca gacgtggccc tgggcgattt
     4441 tatcaatctg ctccggcgtc gaatgacgcg catcagcaac cagcgtgata aagcgtttat
     4501 agccattctc aatgcttaat tgcatcatct gctgcgcttc cggcggcagt gccctggtga
     4561 tagaaacatc cgccagcggt gaagttgaga caccatcagt atgaacacca atcgaatcca
     4621 gactattttc tacggtggtg atcacgccga agataccgat agaaccggtc agggtgctgg
     4681 ggttagccac aatgtaatta gctggcgtgg aaatccagta accaccagat gccgccatgc
     4741 cgcccatcga tacaaccaca ggcttacccg ctgcccgggc tgctgccagt tcagcgcgaa
     4801 tcacttcaga cgcggtaacg ctgccgcctg ggctattaac acgcaggaca atcgctttca
     4861 ctttcgggtc aaggcgagcg tcgcggattt gtgccgcagt ggtatcaccg ccaacattcc
     4921 cctgagtttc ctcgccatcc ataattgcgc cattagcgaa gacgacaccg atgctgtcac
     4981 cggtatctgc cggcgttttc aatgcgtaat cgtaataact gatggcgcga taatttttat
     5041 cagtcttact ccagccgaac tctttggtta gagctttttc gatttcggca ctcgatgcca
     5101 gtgcatcgac cagcttgttt tccagtgcat atttcgcggt atcgccaccg gttttggtta
     5161 aaccctcaag caacccttgc gcgccaggga atacctgctg agcagggatc tgccggttag
     5221 cggcaacagt attcagatag ttttgccaca gctcaccaat ccagcggctg tcagcttcgc
     5281 gggctgccgg tgacatgtca tcacgaataa acggttcaac ggcagattta tacgtaccca
     5341 cgcggaacac atgggtggaa actttcagct tatccagcaa cgatttgtag tacagaccgt
     5401 tggtagcaaa accgtgcaaa tccaccacgc cttgcggtga cagccaaatt ttattggcga
     5461 aactggcgag ataatattgc ccctggctgt agttctcgcc aacggcataa accggtttcc
     5521 cgctgtcacg aaactctttc agggctttgc cgatgtactg catagacggc tggtcgccgc
     5581 ctgcgaagtt tttcagatcc atcacgatac cggtgatatt gcggtcgtcc ttcgcctggc
     5641 gaatggtgtt gacgatatca aacagtgagt tttcctgcag acgatcggaa ctggcaccaa
     5701 gcagctggcg gcttaattta ctaaaccgct gagaactgtc gggtttatcg acgatcacac
     5761 cagaaatgtc cagcagcagt gcgccacgac tggccgtttc tttcgaatca ccaccactga
     5821 cctgcatcca gatccccaca ccaaccagta cgaggaaaat aaagaacagg ttaagtacca
     5881 tttcacggac gaaattcagc agacgccacg tccatttaaa aaatccggca ataaatcgcc
     5941 aaagggttcg catgtattct cccaacttaa ggtcacacct gtcacaggcg caatagatgt
     6001 ggttatcgta atgacacaac cggcacttgt cagcaggaat tgcggactgt gctgtaacaa
     6061 aatccactct cgtgttaatt ttgtgaataa atatcacgac aggagttaat caaatggatg
     6121 cactcgaact attgatcaat cgccgtagcg cctcccgcct ggctgaaccc gcgccaacgg
     6181 gtgaacaact gcaaaacatc ctgcgtgcgg gtatgcgtgc gccggaccat aagtccatgc
     6241 aaccgtggca tttttttgtg attgaagggg aagggcgcga gcgtttcagc gccgtactgg
     6301 aacagggggc gattgctgcc ggtagtgatg acaaagctat cgacaaagcc cgtaatgcgc
     6361 cgttccgcgc accgctcatc atcacggtgg tggcgaaatg cgaagaaaat cataaagtcc
     6421 cgcgatggga acaggaaatg tctgccggat gcgcggtcat ggcgatgcaa atggcagccg
     6481 ttgcccaggg atttggcggc atctggcgca gtggcgcatt aactgaaagt ccggtagtgc
     6541 gtgaagcatt cggttgccgt gagcaggata aaattgtcgg ttttctctac ctcggcacgc
     6601 cgcagttgaa agcatctacg tcgattaacg tcccggaccc gacgccgttt gtaacttatt
     6661 tctgataatt gtcgcgaaac tgtctggatt gtgagcaaag gcgcaggaat tcagacactc
     6721 tcacttatca cttcacggaa tgagggttac catacccgga ttgcattgac aggagatgtc
     6781 catgagcgag aactcgattc gtttgaccca atacagccac ggagctggtt gcggctgtaa
     6841 aatttcccca aaagtgttgg aaaccatcct gcacagtgag caggcgaagt ttgttgatcc
     6901 gaatttgctt gtgggtaatg agacccgcga cgatgcggcg gtgtacgatc tgggcaatgg
     6961 caccagcgtt atcagtacca ccgacttctt tatgccgatc gttgataatc ctttcgattt
     7021 tggccgcatt gcggcgacta acgccatcag cgatatcttc gcgatggggg gcaaaccgat
     7081 tatggcgatt gcgatcctcg gctggccgat taacaaactt tccccggaaa ttgcccgcga
     7141 agtaaccgaa ggtggacgct atgcatgccg tcaggcgggt attgcgctgg ctggcggtca
     7201 ctccatcgat gcgacggagc cgatttttgg tctggcggta acggggatcg taccgaccga
     7261 gcgggtgaag aaaaacagta ccgcacaagc cggatgcaaa ctgttcctga cgaaaccgct
     7321 ggggatcggc gttcttacca cggctgagaa aaaatcactg ctgaaaccag aacatcaggg
     7381 actagcgacg gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg ccaacatcga
     7441 aggcgtaaaa gcgatgaccg acgttacggg ttttggtctg ctgggccatt tgagcgaaat
     7501 gtgtcagggc gcaggtgtgc aggcgcgcgt tgactatgac gcgatcccaa aattgccggg
     7561 ggttgaagag tacattaagt tgggcgcagt gcctggcggc accgaacgta actttgccag
     7621 ctacggtcat ctgatgggtg aaatgccgcg tgaagtgcgc gatttgctgt gcgatccgca
     7681 aacttctggc ggtttgctgc tggcggtcat gccggaagca gaaaatgagg tcaaagctac
     7741 agccgccgag tttggcattg aactgacggc aattggcgaa ctggtgccag cgcgcggcgg
     7801 tcgtgccatg gttgagattc gttaattcaa tgcggttgtt tattgccgaa aaaccgagtc
     7861 tggcgcgcgc cattgctgat gtcctgccca aaccgcaccg gaaaggcgat ggctttatcg
     7921 agtgcggtaa tggtcaggtg gtgacctggt gtatcggtca cctgcttgag caggcgcagc
     7981 cagacgccta cgacagccgc tatgcgcgct ggaatcttgc ggatttgccg attatcccgg
     8041 aaaagtggca attacagccc cgaccctccg tgaccaaaca acttaacgtc atcaaacgct
     8101 tcctgcatga agccagcgaa atcgttcacg ccggggaccc ggatcgtgaa gggcaactgc
     8161 tggtggatga agtgctggac tatctgcaac tggcaccgga aaagcgccag caggtgcagc
     8221 gttgcttgat aaacgacctg aacccgcagg cggttgagcg ggcgctcgac cgtcttcggt
     8281 ccaacagtga gtttgtgcca ttgtgcgttt ctgcgctggc gcgagcgcgt gccgactggc
     8341 tgtacggcat caatatgacc cgtgcgtata ccattctcgg tcgcaatgcc ggttatcagg
     8401 gcgtactttc cgtgggacgc gtgcagacac ccgtgctcgg gctggtggtg cgccgcgatg
     8461 aagagattga aaacttcgtg gcgaaagact tctttgaagt caaagcacat atcgtgacac
     8521 ctgccgatga gcggtttacc gctatctggc aaccgagcga agcgtgtgaa ccgtaccagg
     8581 atgaagaagg gcgcttgtta catcgtccac tggcggagca tgtggttaac cgcattagtg
     8641 gtcaaccagc aattgtcacc agctataacg ataaacggga atcagaatcc gcgccgctgc
     8701 ctttttcgct ttcggcgttg cagattgaag cggcaaaacg ctttggtctg agcgcgcaga
     8761 acgtgcttga tatctgccag aagctgtacg aaacgcacaa gctaatcact tatccgcgtt
     8821 ccgattgtcg ctatttgcca gaagaacatt ttgccggacg ccacgcggtg atgaatgcca
     8881 tcagcgttca tgcaccagat ctgttgctgc agccagtggt agatccagat atacgcaacc
     8941 gctgttggga tgacaaaaag gtcgatgcac accacgccat catcccgacc gcacggagtt
     9001 cagccatcaa cctgacggag aacgaagcga aggtctataa cctgattgcc cgtcagtact
     9061 tgatgcagtt ctgcccggat gtggtgttcc gcaagtgtgt tatcgaactg gacattgcca
     9121 aaggcaaatt tgtcgctaaa gcgcgttttc ttgctgaagc aggctggcgc acgctgttag
     9181 gcagcaaaga gcgcgatgaa gaaaacgacg gtacgccatt gcctgtggtg gcgaaaggcg
     9241 atgagttgct gtgtgaaaaa ggtgaagtgg tagagcggca aacccagccg ccgcgccatt
     9301 ttaccgatgc aacactgctt tcggcgatga ccgggatcgc gcgctttgtg caggacaaag
     9361 atctgaaaaa gatccttcgt gcgaccgatg gtctggggac agaagcaacc cgtgccggga
     9421 ttattgaact gctgttcaag cgtggttttc tgaccaaaaa agggcgctat atccactcta
     9481 ccgacgccgg aaaagcgtta ttccattcgc tgccagaaat ggcgacgcga ccggacatga
     9541 ccgcgcactg ggaatcggtg ctgacgcaaa tcagcgaaaa gcagtgtcgc tatcaggact
     9601 ttatgcagcc gctggtgggg acgctatatc agcttattga tcaagccaaa cgtacgccgg
     9661 tgcggcagtt tcgcggcatt gtggctccgg gcagtggtgg cagtgctgat aagaaaaagg
     9721 ctgcaccgcg taaacgtagt gcgaaaaaaa gtccgccagc agatgaagtc ggaagcgggg
     9781 cgatagcgta agcgagtgaa tctttcgtgc tattcgagtc
//
LOCUS       DAJTYZ010000141         9565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000141 DAJTYZ010000000
VERSION     DAJTYZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.141"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..1344
                     /locus_tag="OO596_003432"
                     /pseudo
     CDS             19..1344
                     /locus_tag="OO596_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            1347..2675
                     /gene="ssnA"
                     /locus_tag="OO596_003433"
     CDS             1347..2675
                     /gene="ssnA"
                     /locus_tag="OO596_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCR6719208.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2726..3505
                     /locus_tag="OO596_003434"
     CDS             2726..3505
                     /locus_tag="OO596_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCR6719209.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3502..6441
                     /locus_tag="OO596_003435"
     CDS             3502..6441
                     /locus_tag="OO596_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCR6719210.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6374..6688
                     /locus_tag="OO596_003436"
     CDS             6374..6688
                     /locus_tag="OO596_003436"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR6719211.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6724..7074
                     /gene="ghxQ"
                     /locus_tag="OO596_003437"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6724..7074
                     /gene="ghxQ"
                     /locus_tag="OO596_003437"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7143..7631)
                     /locus_tag="OO596_003438"
     CDS             complement(7143..7631)
                     /locus_tag="OO596_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6719212.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7631..9550)
                     /gene="ygfT"
                     /locus_tag="OO596_003439"
     CDS             complement(7631..9550)
                     /gene="ygfT"
                     /locus_tag="OO596_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCR6719213.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2307 a         2564 c         2492 g         2202 t
ORIGIN      
        1 gatccttaac tgatcggcat tattcctcag ttccgtattc ctgcagagtt aattcagcac
       61 gatatcgatt ttgttgccgc tcacggcgtg aaatttgagt atggctgctc acccgattta
      121 accgttgaac agttaaaaaa tcagggcttc cactatgttc tgattgccac cggcactgat
      181 aaaaatagcg gtgtgaaact ggcgggcgac aaccaaaatg tctggaaatc actccccttc
      241 ctgcgtgaat acaacaaggg tacagcgctc aagctgggca aacatgtggt cgttgtcggg
      301 gcgggtaaca ccgcaatgga ctgcgctcgt gcggcgttac gcgttccagg cgtagaaaaa
      361 gcaacgatcg tttaccgtcg ttcactacaa gagatgcccg catggcgcga agagtatgaa
      421 gaagcgttgc acgacggcgt agagttccgt ttcctgaata atccggaacg tttcgatgct
      481 gatggcacct taaccttgcg cgttatgtcg cttggcgaac cggatgagaa aggtcgtcgc
      541 cgtccggttg aaaccaacga aacagtaaca ctgcatgtag acagcctgat caccgccatt
      601 ggtgaacagc aggatactga agccctgaat gcgatgggcg tgccgctgga caaaaacggc
      661 tggccagacg tcgaccataa tggcgaaact cgtctgactg acgtctttat gatcggcgac
      721 gtacagcgcg gaccatcctc cattgtcgct gctgtcggaa ccgcgcgtcg ggcgaccgat
      781 gccatcctta gtcgggaaaa tatccgttcc caccagaacg ataaatactg gaacaacgtc
      841 aatccagcgg aaatctatca acgtaaaggc gatatctcta tcacgctggt gaacagtgac
      901 gatcgtgacg cgtttgtcgc ccaggaagcc gctcgctgcc tcgaatgtaa ctacgtttgc
      961 aacaagtgtg tggatgtctg cccgaaccgc gccaacgtct ccattgcagt ccctggcttc
     1021 cagaatagat tccagacgct gcacctcgac gcttactgta acgaatgcgg caactgcgct
     1081 cagttctgtc cgtggaacgg taaaccatac gaagacaaaa tcaccgtctt cagcctggcg
     1141 caagactttg ataacagcag caccccaggc ttccttgtgg aagattgccg ggtacgagta
     1201 cgtctgaata accaaagctg ggtgttaaac atcgacagcg aaggtcagtt taacaacgta
     1261 ccaccggagc tgaacgatat gtgccgcatc atcagccatg tccaccagca tcatcattat
     1321 ctgctgggcc gcgtggaggt gtaatcatgt tgattctgaa gaatgtcacc gcagtgcagt
     1381 tacacccggc gaaagtgcag gaaggcgttg atatcgctat cgaaaatgac gtgattgtcg
     1441 ctatcggcga tgccctgacg caacgctacc ccgatgccag ctacaaagag atgcatggtc
     1501 ggattgtgat gccgggaatt gtctgctcgc acaaccattt ttactcggag ctttcccgcg
     1561 gaattatggc aaacatcgcc ccttgcccgg atttcatctc aacgctgaaa aatctctggt
     1621 ggcggctcga tcgcgccctt gatgaagagt cgctctatta cagcggactg atttgttccc
     1681 tggaagcgat taagagcgga tgtacatcgg ttatcgatca ccatgcctcg ccggcgtata
     1741 tcggcgggtc gctctccaca ttgcgcgacg catttttaaa agttggcctg cgcgcgatga
     1801 cctgttttga aactactgac cgtaacaacg gcatcaaaga gttgcaggaa ggtgtagaag
     1861 aaaacatccg tttcgcccgt ttgattgatg aggcgaagaa agcgacaagc gagccgtatc
     1921 tggtggaagc acatatcggt gctcacgcgc cgtttaccgt gccggatgcc ggtctggaga
     1981 tgctgcgtga agccgtgaaa gccacaggcc gtggtttgca tattcacgct gcggaagacc
     2041 tttacgacgt ttcctacagt caccactggt acggcaaaga cctgctggca cgactggcgc
     2101 aattcgacct catcgacagc aaaacgctgg tcgctcatgg gctgtacttg tcgaaagatg
     2161 acatcgccct actcaatcag cgcgatgcgt tcctggtgca taacgcccgt tcaaacatga
     2221 acaaccatgt cggctacaac catcacctta gcgacatccg caacctggca ttgggaacgg
     2281 acggcattgg ttcggacatg tttgaagaga tgaaatttgc cttctttaaa catcgcgatg
     2341 cgggtggtcc gctgtggcct gacagttttg ccaaagccct gactaacggt aacgaactga
     2401 tgagccgcaa ctttggcgcg aaatttgggc ttctggaagc cggttacaaa gctgatttga
     2461 ccatttgcga ttacaactcg ccgacgccgc tgctggcaga caatatcgcc gggcatatcg
     2521 ctttcggtat gggctcaggc agcgttcaca gcgtgatggt caatggtgtg atggtctatg
     2581 aagaccgtca gtttaacttc gattgcgatt ccatttatgc acaagccaga aaagccgctg
     2641 ccagtatgtg gcgtcggatg gatgcgctgg cataaatgac agatgccctc ttcccgcagg
     2701 gaagagggtt aaataaggaa agaagatgat tgaacaattt ttcaggcccg actctgtcga
     2761 acaggcgctg gaactgaagc gccgctacca ggatgaagcc gtctggttcg ccgggggcag
     2821 caaactcaat gctacaccaa cccgtaccga taaaaagatt gccatttcct tacaggatct
     2881 ggaactggac tgggttgact gggataacgg tgcactgcgg attggtgcaa tgtctcgctt
     2941 gcagccactg cgtgatgcgc gctttattcc tgcagcactg cgtgaagccc tcggttttgt
     3001 ttactcacgc catgttcgta atcagtcgac cattggtggt gaaatcgccg cccggcagga
     3061 agagtcagtg ctgcttcccg tcctgctagc actggatgct gaactggttt ttggcaacgg
     3121 cgaaacgctg tcaatcgagg actacctggc ctgcccatgc gatcgcctgc taaccgaaat
     3181 tatcattaaa gatccgtatc gcacctgtgc gacccgcaaa attagccgtt ctcaggcagg
     3241 tttaaccgtc gtgacggcag ccgttgcaat gacagaccac gacggtatgc gaattgcgct
     3301 ggatggcgtc gccagtaaag cactgcgtct gcacgatgtc gaaaaacaaa atctggaagg
     3361 caatgcactt gaacaggctg tcgccaacgc cattttcccg caggaagatt tgcggggcag
     3421 cgtggcctat aaacgctata tcacgggagt tctggtagcc gacctgtatg ccgactgcca
     3481 acaggctggg gaggaagccg tatgatcatc cactttactt taaatggcgc gcctcaggag
     3541 ctaaccgtta atccaggcga aaacgtgcaa aagctgttgt ttaacatggg aatgcactct
     3601 gtacgcaaca gtgatgatgg cttcgggttt gccggttctg acgcaataat ctttaacggt
     3661 aatatcgtta acgcgtcctt gcttattgcc gcacagttag agaaggcaga tattcgtacc
     3721 gcagaatctc tgggtaaatg gaacgagtta agtctggttc aacaggcaat ggttgatgtt
     3781 ggcgtggtgc agtctggtta taacgatcca gctgcagctc tgattatcac cgatcttctc
     3841 gatcgcatcg ccgcacctac ccgcgaagag atcgacgacg cgctttctgg tttgttcagc
     3901 cgcgatgcag gctggcaaca atactatcag gtcattgaac tggcggttgc acgtaaaaat
     3961 aatccgcagg ccaccattga tatcgctccg actttccgtg acgaccttga cgtcattggc
     4021 aagcattatc ctaaaactga tgccgcgaaa atggtgcagg cgaaaccctg ctatgttgaa
     4081 gactgcgtaa cggctgacgc ctgcgtcatt aaaatgttac gtagcccaca cgctcacgca
     4141 ctgattactc atctggatgt cagcaaagct gaagccttac cgggcgtcgt tcacgttatt
     4201 actcacctga attgccctga tatctactat accccggggg gtcagagcgc accggaaccg
     4261 tcaccgcttg accgccgtat gttcggcaag aaaatgcgtc acgtcggcga tcgtgttgct
     4321 gcggtcgtcg cagaaagtga agaaattgcg ctcgaagcac tgaagctcat cgacgttgaa
     4381 tatgaagtgc ttaagccggt aatgtcgatc gacgaagcaa tggcggaaga tgcgcctgtc
     4441 gtgcacgatg aaccggtggt gtatgttgct ggtgcgccag atactctgga agacgataac
     4501 agccatgcag cccagcgcgg cgagcatatg atcatcaact tcccgatcgg ttctcgccct
     4561 cgcaaaaata tcgccgccag tattcatggt catattggcg atatggacaa aggctttgcc
     4621 gatgccgatg tgatcattga gcgaacctat aactcaacgc aggcacagca gtgcccgact
     4681 gaaacacata tctgctttac ccggatggac ggcgatcgtc tggttatcca cgcctccacc
     4741 caggtaccat ggcacttacg ccgccaggtc gcgcgcctcg tgggcatgaa acagcataaa
     4801 gttcatgtca ttaaagagcg agttggcggc ggttttggtt ccaaacagga catcctgctg
     4861 gaagaagtgt gcgcctgggc aacctgcgtg accgggcgtc cggtactatt ccgctacacc
     4921 cgtgaagaag agtttattgc taacacctct cgtcacgtcg cgaaagtcac cgtcaaactg
     4981 ggagcgaaaa aagatggtcg cctgacggca gtgaagatgg atttccgcgc caacactggc
     5041 ccttacggca accactcact caccgtaccg tgtaacggac cggcgctgtc gctgccgtta
     5101 tatccgtgcg ataacgtcga tttccaggtc accacctact acagcaacat ttgcccaaat
     5161 ggtgcttatc agggttatgg cgcaccgaaa ggtaacttcg ctatcaccat ggcattagcg
     5221 gaactggctg aacagttaca gatcgaccaa ctggaaatta tcgaacgtaa ccgggtacac
     5281 gaagggcaag agctgaaaat tctcggtgca atcggtgaag gtaaagcgcc gacctccgtt
     5341 ccttccgccg ccagctgtgc actggaagag atcctgcgtc agggtcgcga gatgatccaa
     5401 tggtcgtcac cgaaaccgca aaatggtgac tggcacatcg gtcgcggcgt cgccattatc
     5461 atgcagaaat cgggtatccc ggatatcgat caggctaact gcatgatcaa actggaatcg
     5521 gacggcacct ttatcgttca ttctggcggt gcggatattg gtactggtct ggatactgta
     5581 gtgacgaaac tggcagcaga agtgctgcac tgcccaccgc aggacgtgca tgttatctcc
     5641 ggtgataccg atcatgcgtt gtttgataaa ggcgcatatg cctcgtccgg tacttgcttc
     5701 tcgggtaacg cggcgcgttt ggcagcggaa aatctacggg agaaaattct gttccacggc
     5761 gcgcaaatgt tgggtgaggc agtggcagat gttcaactgg caacgccggg cgtcgtgcgc
     5821 ggcaagaaag gcgaagttag tttcggggat attgcccata aaggcgaaac cggcatcggc
     5881 tttggttcac tggtgggaac tggcagttat atcacgcctg atttcgcctt cccgtatggc
     5941 gcaaacttcg ctgaagttgc cgtcaacacg cgtacgggtg aaatccgcct ggataaattc
     6001 tacgccttgc tggactgcgg tacaccggtc aacccggaat tagcattggg acaaatctac
     6061 ggtgctaccc tgcgagctat cggccacagt atgagcgaag agatcattta tgacgccgaa
     6121 ggtcacccgt taacgcgtga tttacgcagt tacggcgcac cgaaaattgg tgacattccg
     6181 cgtgatttcc gcgctgtgct ggtgccgagc gacgataaag tcggcccgtt cggggcgaaa
     6241 tcgatctcgg aaatcggtgt aaatggcgca gctccggcga ttgctaccgc aattcacgat
     6301 acatgcggca tctgggcaga agaaagtcaa agtgggcatg ggaacggata tcggtgccgg
     6361 aacaactttc aacatgctgc aaacgctgaa cgaagcctac aaagtgttgc aattacaagg
     6421 ctatcgcctc tcggcatatg aagcgtttta cctggccacg ctcggcggag cgaaatctct
     6481 gggccttgac gatttgattg gcaacttttt acctggcaaa gaggctgatt tcgtggtgat
     6541 ggaacccacc gccactccgc tacagcagct gcgctatgac aactctgttt ctttagtcga
     6601 caaattgttc gtgatgatga cgttgggcga tgaccgttcg atctaccgca cctacgttga
     6661 tggtcgtctg gtgtacgaac gcaactaata ataaaacttt aacatcctcg tgaggacatc
     6721 attatgtctg gagacatcct acaaacaccg gacgcaccaa agccacaggg cgcgctggat
     6781 aattatttta aaattaccgc tcgtggcagt accgttcgtc aggaagtact ggctggctta
     6841 acgacctttc tggccatggt ttattccgtt atcgtcgttc cgggaatgct gggcaaagca
     6901 gtttttcctc ccgcagctgt gtttgttgcc acctgtctgg tcgcgggctt cggctcgttg
     6961 ctgatggggt tgtaggccaa tttgccaatg gcgattggtt gtgcgatttc cttgacggca
     7021 tttaccgcat tcagtctggt actcgggcaa caaattagcg ttcctgtcgc actggtcgca
     7081 ttttacgcgg gtggttgggc aatctaatag attctccgcg cctttcagcg cggagatttc
     7141 tttcacagag gattctcacc gctggcaaca atacgctgct ggcggactcg cctgagttct
     7201 ctttcagtaa gcaactgcag cgcctgtgtc gggcaagatt caacacaggc tggcccctct
     7261 tcccgctgtt cacagagatc gcatttcaca atttgttgtc gggtatcccc cggcaacgac
     7321 tgaatggtga tcattccaaa cgggcacgca ctgacgcaac tctgacagcc aatacaacgg
     7381 gcagaattgg tctgcaccac ctgctcgccc atcgtcagcg cccccacggg gcaagcgcca
     7441 acacaagggg cgttttcaca ctgatggcac atcaccggcg cactgatgct gtccagtcgc
     7501 tgtaccttca gccggggtaa aaagacatcg gcattcaact cctgttctga agggtgagcg
     7561 accacacagg ctacttcaca agtacggcag ccaatacagt cagccggatt aacgataatt
     7621 aacgatttca tcacgatgcc ttcgtatcaa acagagttaa catatcgcgc gccgcctgcc
     7681 ttcctgcggc cattgcagtg acaaccagat ccgcgccatg aactgcatca ccaccagcaa
     7741 agactttttt cagatgcgtc tgggtaggta aatacccgac gtcaccggtt tgaatcaggc
     7801 cccatttatc gagtttaatt ccgctgccct gcaaccacgg catggcatgc gcctggaaac
     7861 caaaggccat aatcagaaca tcggcgggca attcaaactc tgaacccgct accggacgag
     7921 gacgacggcg accatccggc cccggctcac ccatggcggt acgaatcagg cccaccgcag
     7981 ttaaacgccc atcttcgtca caagcgatat attgcggttg aacattgaac tgaaactcga
     8041 caccttcctc gcgcgcatta accacctctt tgcgcgagcc cggcatactg acttcatcac
     8101 gacgatacgc gcaggtcacg ctggcagcat tgaggcgaat ggaagtccgc aaacaatcca
     8161 ttgtcgtatc gccaccgccc aataccacga ctcgcttacc ttccacgtcc gtcagcggat
     8221 actcttcaga ctccggcaat cccatgagct ggcgggtatg ggcagtcagg aacggtagag
     8281 cctgaatgac accgggcgca tcttcatgcg gcagatctgc tcgcatcatc ccgtaagtcc
     8341 ccacgccgat gaaaactgca tcatattcag aagttaaatc gctaaaggta atatcgcggc
     8401 caatttcaca gttaagatga aagtcgattc ccattgcggt gaatatctct cgccgctggc
     8461 ttaataccgt tttatcgagt ttgaaaggag gaatgccaaa agtcagcata ccgccaattt
     8521 ctggatggcg atcaaagaca tcaacctgaa ctcccgcgcg cgccagaata tcagcacacc
     8581 ctaaccctgc tggcccagcg ccaatcaccg ccactttttc gatacgggga acaactttgc
     8641 tgacatcggg acgccagccc atcgccagcg cggtatcggt gatgtagcgt tccagattac
     8701 cgatagagac tgcgccagag tgatctttca aagtacatgc gccttcacaa agacggtcct
     8761 gtgggcatac cctgccgcag atttctggta aggaactggt ctggtggcaa agttctgccg
     8821 cttcaataat ctttccttcc tgtaccagac ggatgtaatc cggaatagcg ttatgcagcg
     8881 ggcaatgcca gttgcagtta gctttttcgg cacaataaac acagcggtca ctctcataag
     8941 tcgcttgcag tggatccagc ccgcaataga tttcgccaaa gtgggttttc cgttcactcg
     9001 ctgaaatttt atctgcgcct ttacgcgagt taacggggag caacgctgca ctgcgagatg
     9061 gctgagcgtc tgatgacgct tttcctgctg ccgttttgcg ctggcgagcc acctttatcc
     9121 actgtaaccc tttatcgtcc atcagtcgta acgcctgtgt tgggcagact tcaatacagg
     9181 cttgcgtgcc ggaactgcgc tggttacaaa ggtcgcattt ctgcgcaatc gtatcgacca
     9241 tctcaacgac gccaaagggg caagcgattg cgcatctttt acagccaata catttttgct
     9301 cgtccagttg tacgctatcg gactggaaag tcagagcatt aaccggacaa gccgtaacac
     9361 aaggggcatt gttgcagtga tggcaggcca ccggattcgc agcctggcct ttccctacaa
     9421 cgtggatacg cggtcgaaag tcactgtgac tcagcggcca gttttcttga ttgtgtgcca
     9481 ccgcacaggc aatttcacaa gcatgacagc ctatacattc cgcagcttca gcagcgataa
     9541 acttattcat ttgcatccct ttcat
//
LOCUS       DAJTYZ010000142         9502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000142 DAJTYZ010000000
VERSION     DAJTYZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9502
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.142"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(150..1232)
                     /locus_tag="OO596_003440"
     CDS             complement(150..1232)
                     /locus_tag="OO596_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCR6719214.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1229..3025)
                     /locus_tag="OO596_003441"
     CDS             complement(1229..3025)
                     /locus_tag="OO596_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6719215.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3276..3632)
                     /locus_tag="OO596_003442"
     CDS             complement(3276..3632)
                     /locus_tag="OO596_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719216.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3690..4112)
                     /locus_tag="OO596_003443"
     CDS             complement(3690..4112)
                     /locus_tag="OO596_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6719217.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4128..4853)
                     /locus_tag="OO596_003444"
     CDS             complement(4128..4853)
                     /locus_tag="OO596_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR6719218.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4875..5621)
                     /locus_tag="OO596_003445"
     CDS             complement(4875..5621)
                     /locus_tag="OO596_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6719219.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5628..6692)
                     /locus_tag="OO596_003446"
     CDS             complement(5628..6692)
                     /locus_tag="OO596_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR6719220.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6764..7189)
                     /locus_tag="OO596_003447"
     CDS             complement(6764..7189)
                     /locus_tag="OO596_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6719221.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7186..7449)
                     /locus_tag="OO596_003448"
     CDS             complement(7186..7449)
                     /locus_tag="OO596_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6719222.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7561..8061
                     /locus_tag="OO596_003449"
     CDS             7561..8061
                     /locus_tag="OO596_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6719223.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8081..8275
                     /locus_tag="OO596_003450"
     CDS             8081..8275
                     /locus_tag="OO596_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR6719224.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8275..8565
                     /locus_tag="OO596_003451"
     CDS             8275..8565
                     /locus_tag="OO596_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6719225.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8845..8997
                     /locus_tag="OO596_003452"
     CDS             8845..8997
                     /locus_tag="OO596_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6719226.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            8997..9374
                     /locus_tag="OO596_003453"
     CDS             8997..9374
                     /locus_tag="OO596_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719227.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT         2444 a         2494 c         2016 g         2548 t
ORIGIN      
        1 ccacagaaca attattgctt ttgccgttga tgacaataaa gtttttatcg caggcgtata
       61 ccacggtggg caaatctatg aaaccgattt tttatccagt gcaccgtaaa acccccatcc
      121 ttcagggcgt ggcggatatc aaactttggt cattttctct ggctgatctt taccggataa
      181 ccatcccgaa gatcaattaa gtgctccggt tcagaagcaa accatgcata agatccccac
      241 gccaattctg gtattgcctt tttaaaaggc agagcacttc tgtccataaa tgccgtaagg
      301 aatgcaagat ttttctcgct gaacccagcg tcaatagcca aatgagtgag agtaactttt
      361 cgttccctgt ttataggccc atctgtcgca actacttcag tgaaaactac aagcatgtcc
      421 gaacccgttc ggtcctctcc caaatcaatc aaaataatat ctggaagtgc ttttgaggca
      481 tcaatattca gccccaatgc ttttgccaat acatcatcct gagcaacaac tttattcccg
      541 gattcagaca accagagcac agttggattc ttaagaaaat taggagcaaa aacttcgatg
      601 acggccttag ctattgtact tgacgggccc ggagctaaag ttctcttctc tccatttggg
      661 aaagtgacga ctacggcatc agcagcaatc actgcccctg atttcaataa acgcaaccta
      721 gcaagagcgg ctttgttcaa atgattttct tgccaggaac ttattgcact cagcagcgaa
      781 tctccttcaa gcccggcatc aaagagtgct gcaaaagagc gattcaaact gtaacgagga
      841 cgagaggatg tggtgggtat acctttacgc tcaacaattg cattgcaagg tattaggcca
      901 ttttttatag tttcatcacg tattggctca cgcgtatttt ccgcatacca tgcatcagca
      961 ggccttatct tttgtttaga gaaagtcatc tttacccagc tttctctgga cgcatcatca
     1021 gtaagagcag cctgcgaatc tcccatactt acaacctggc tgggccttat ccatcgcccc
     1081 agcccctcaa ccgcaccagc ataaaacatg acaaaaattg tttttgcagc tatttcccga
     1141 attaaatatc cacgattctc agtcccttca gggaaaatta attgtaatct ttcccggata
     1201 acattcaatg ggggaacaac aggcagattc atgcaatcat cactccatag aaaccagcga
     1261 tagttctctc aataatccgt ctgtggacgc cagcatcaat taacgattga atctccatca
     1321 cctgctccag agaaggcaac gggattgaat tcaattcata ggccgaaaca gcaacacttc
     1381 cgcttataca cctgaacgcc cgatcgacaa catgcgaatt aagtagcata tcaataacat
     1441 cagggcgaac cgcagagaac aatccgtttg aatatacaat attaatatgg ttttcaacta
     1501 ctacgccacc agtttcatct ataaatccct gaggtaaaat tgccgctaaa atccttctgt
     1561 cctgttcctt cgatgttgtc cgctgaacaa gcacacattc agattttgtc acaaggaaac
     1621 cctgttgtgg ggttatatcg atatagggaa catgattttt tctgtctgca ctaaaacgaa
     1681 aaccagctga agttattgat tccgcccaga ctaatgggta gctatttttt cctttcgtcg
     1741 tacgcaactg cgacttaaat cgattccaga ctaactgacc tgtagagact gagtatccca
     1801 gatcagctaa acgcgtagac atctgtttta atacattaag aaaacaggca tcatctttaa
     1861 cgcgtggcaa aagccacgtt gctccacctt tttctatatc aacattacct attttctcaa
     1921 tttttgcttt gttcaacccc ttagggacaa gtgaggatac ctgggcccga actttatgtt
     1981 ctcccgcctt gaatgctgtc aacattgttt cctggagaac atcgtcaaag accccatccc
     2041 tgtcagtgac gaaatcaagg gcatacggag tagttttttc ggtcaaaagg gttcgcagcg
     2101 cggtgaaata ctgcccacca agaaaagaag ttggcgtaag aaatgcaata actcctgttt
     2161 tctctttaac catgcgaaca gctaagtcgg tgaacaatcc atatagattt gcatgaccaa
     2221 ataacgaacg agaatatttt tctctcgttt taatatcgag agtgacacgt ccgtaaggcg
     2281 gattccctac cacaagatca taaccagaaa atttatcttg ctgaagcgca tcagcaacaa
     2341 taattgtatc ttcaggcaat ctacgtttta cctttacaca tagtggcatt agtacagatt
     2401 cgagcaatac caaactcatc caagccgcaa atggatcgat ctctatcccc tttaaacgac
     2461 gactgattct cctcaacatc cattcagggg aagacccctt gtcttttttt aacattcgaa
     2521 tggctactgg tgccaaaaat gcaccgccac cacacgcagg atcaataacc gatgcatgag
     2581 aaaaatcaac accagacttt tcagccaggt ccaagagacg cgctaccaaa ggtggggggg
     2641 tatagtaagc ccccaattcc gaacggtaag cggatggcag cattaccgta taaattgacc
     2701 caatcagata tccagcgtcc tccgccggaa attgagcaat aagctcacct gttcgttcag
     2761 cgagctcgcg tgcatcacat ggaatgtctt ctatgtaaac agtgtgcggt actgccctca
     2821 ttttaatgct gcttttttgc ttttttgcaa gggtatccca atatgcagct acgatgctga
     2881 agcagtatga gcgggcatgc atcaaccttg actgcccggt tttgtaattg cccgcataac
     2941 ctttggccat ggctttacaa gtttgatatc tttccagatt agaaaactca ttatctgtaa
     3001 aaaaatcaaa atgttgcaca ttcataatgt aaaatcctaa tgtatcaaac atgcgggcca
     3061 taacatctca tggtggcgca tagcaaactg gcccgaagaa tatcacgcac caagtcgatc
     3121 tcataacccg ttatgacaat aaatctttgt agctgaactc acttggtgat ttgtcaatca
     3181 ttcctggcct ccagttcgtc ctgaatctct ttatcgagtt caccgtattt gttagtcctc
     3241 atcactcacg acttgacttc cagaaataac cttgattatt cggggctata ttttgtcacc
     3301 ctgcgtatcc gcgctttcgc attacgctca atctggatta gcttttctat attttttcgc
     3361 ctttcccgct cctcctggcg caagagcctt acatcatctg ccagtctggt ttctcttttc
     3421 gccacagaga gcatccagtc aaatggctcc acaactgcac cgcaaatttt acagcggacc
     3481 tgacgctctt tttcgtcaac ccggacagaa gcgtgatggc aatatggtct ttccgatggc
     3541 tcataaagaa aattaacctg attacgaggg ttatcctctt ttaccggaaa taaaacaata
     3601 ttgcttaact catcttctgg ttttatttcc atgctcctct cctttgatgc gaatgccagc
     3661 ggtaattgaa gcctgatagc taatttcact cacagtaccg cctcctgaaa atcaccctga
     3721 tagaaagccg gtacacgctg cataacttca ctcttccggc actcgcgaca gattatgttc
     3781 tgatgtctgt cgtagcggcg tatttctccg tccggtaatg accagataag gtccggatca
     3841 accacagatg gtttcttcag ctttgccctc gagagttttt tgcgggcgtt ttgccagtcc
     3901 ttacgagcct gttcagacgg gaataacccg tagccagagt tgtatacatc cccactggca
     3961 accagctctc tggcgagaac gcttattaaa tatcttgtcg ccccggtttt agcttccagt
     4021 tgtcgtaacg tctcgcgccc actctggcgt acgagttcaa caacctgccc cttaattttt
     4081 tcccgctctt cctgtgtaaa tacttttgcc ataagcgccc ccggcaatca cttttccgac
     4141 acaatacgac cggaggaatc gacaatctgt cgaacaatat cccggtgctt gttcagctcc
     4201 cgcaacgcgg cgcagacacg ctcccacttc tggacatgac ttttcgcccg acgcagttcg
     4261 cggtttgcca tatgcagcga tggtaaaacc aggtcatccg cttgcgtttc ggtgaacgat
     4321 ggcaacgact gcacaatgtc cgccacagtt tctgttttaa tttcttcctg tgttgcagct
     4381 tcctgtaccg gtaacgcaac acctgctggc tgaggaaaga ctttaccatc cgtttccgct
     4441 atggatgcgg ctttcggctc tgccggtaaa tcagcgcccg gtatgcagta ccgaaattta
     4501 ccgccctgat tcacgcgaat cagacgccct ttgctgattg ccatggccag cgatgaattc
     4561 gcccggcggg aggtaatccc gaacatcagt gccagctcat ccgccgtttg tgggccatgt
     4621 tgttcaatcg cctcagtcag catttgcgct gtcactttcg gtaccggtga cactggttca
     4681 ctttcaccag cctgaatcag ccaccacatc gaacccttgt tatccgcttc accacggcgc
     4741 ttcagtttcc acagttcgtt gaccgcatct tcacggctga ttccaaggcg ggacgccacc
     4801 acatgtgaag aggctctttt cagtgctttc agtgcgtcaa atacagtttc cattaaaaca
     4861 tcctccaaca aaaattactt cacaactccc ggaaagctga catttgaacg ccagctatcc
     4921 cagttaaacg tcacccaccg acaaccgttc atggtcatgc ggtccataat cctctcacca
     4981 agaagcgtgc tcattgcggc atgattcagg tttgttaaca tcccgacact gcacagtgat
     5041 gctgtccggc gatcaattat ctggtgcaat accacctgct cgtttttcgt ctcccgctga
     5101 atgcctattt catccaggac cagcaaatca accccgcaaa gctcctgtaa aaatttttca
     5161 ccggatttgc cgttgtcgta gctgtcatgc aacacgctca tgacatcaga ctcggtgacg
     5221 ataatcacgc tgcgcccctt cgccatcagc cggttaccca tcgccgctgc aaggtgattt
     5281 ttcccggtgc cggttttacc gctgaacaca aaattcgtgc acccggtcat cagttcgtca
     5341 gctatggatt tggcctggct cagcgcgtat ttttgcccgt cgttctgcac ctgataattc
     5401 gcaaacgagc atttgctgtg cagaggctgg atgcccgaac gattcaggat tttttccacc
     5461 cgcaactggc gattctggcg gttaatctcc tcgctgcgtt ttcgtccttc agcaagttgc
     5521 cattcccgcc actcctccac cgtccggtac ggtggaaccg ccccctgtgg tgcaagtctg
     5581 tgaatacgtt caagaacccc ggctgccgca atgtttttca tgccacatca ccccctgaat
     5641 cccggcggaa tttcggtatc cggttcagaa atatgattca cacaacgctg gttgttcgtg
     5701 ccgcttaccg ggagcaacca ggggttttca aaattccggt ccggcccaaa aaacgtcgtc
     5761 gctcgctgaa caaattccgt tcccgctttc ccggtcgccg ccaggtatct cgcgtaacgc
     5821 ctcacaccat ccagcatggt ctctggtggc accccctcgc gcaatctggc cttccaggca
     5881 ctgaatgcgg atttcttcgg gtttgccccg gcacgcaacg ggtattcccg ccagacctgt
     5941 tcgaacacat ccggataatc cactcgtccc acaggctgcc cggtgttttc cggggctacc
     6001 cgatcggctt cccgctgaat ggcggaatcg gcttcaggct gctgcagttg gagtgattgc
     6061 tccggccttg cggtcatcac ctgctgcaca gcgcccgaat cggctttcag cgcatacgct
     6121 gaatcggctt ccggtgtcga gcctgctggc tgaccaggat tgacggtctg aacatcccct
     6181 gcctggttcg tggcgttttt tacgccatgg accatagtgt tttgatcttc tttatctgta
     6241 tctttatctg tatctgtcgt gactcgtcgt gacatgtgcg tgacatttcg tgacgcgccg
     6301 tgacaatcgc cattttgttc ccgctttctt tccctctctc gctgcgccct cttgcgctct
     6361 gcaggagatt ttgcggtttg cgaaatattg ccgttgtcct ctttaagcac ctggcgtttt
     6421 tcccatccag tgattaaatc accatcaagt acccgcccct gcatcgtctg caaaattgaa
     6481 tcaattacct cttctgtcac gtcgagcgca cttgccaaat cttctgtcgt gacatcaatg
     6541 tgacctcgcg tgacatttcg tgacgcgctc accaggaggt ggatatacac tgccatcact
     6601 gttgcaattg gctgccctga caccctggca attgttcgcc acttagggtc atttggcatg
     6661 tcatgccata atctgagcca ggcgttagcc atactcacct cttctgatac cgaatctttt
     6721 tactcacgag ttgccgggag cgattcgata tggctattgt cagtcaatgt actgccacag
     6781 catttcctgc cgggccacca cggttcatct gattgaaacc ggcgattgcc actgcgacaa
     6841 aatcatcagc gtctctcacc agtcgttcat gcgtctccac cagttcccga aaataagctg
     6901 aactgtggct gcgcattcga gccaccagcg gaggtggcat tgccctttcg atcgcaggta
     6961 acaacgcctg aattttttca acagcgtcag gggtgtcttt atccagccaa cggaaaatct
     7021 tttgggtgtt gagataaagc gtgttgtgct tgctgtcgtt atgcagaacc ggaaacgtca
     7081 ttcccagttc gaaataaacc ctggttattt cagctgccgg aactttttca ccgtccggat
     7141 gcgcccaggc tttcatcgcc atgcggatgt gttcatgctt gattttcatg aatcaacccc
     7201 cgccgatggt tgtgtgttag cctgaaactc agcaggcaag ccgtcagttg ggttggggta
     7261 ggttttactg tcaatctcat gtggggtcac cacccatccc gttgcttcac accaacgtaa
     7321 tattttttct cctgtaagtt ttgcacgccc cgttataacg tggctgacca tcccttggga
     7381 tgcgccaaca atctcagcaa atgaactctg ggttatatga aaatgatgca aatattctcc
     7441 aagattcata atccgcccta tgtgatgtgt taccaatgca taattaatag cactgttatt
     7501 tttaaaagta aatagtgata ctatttcaaa gtgattagca atcttattag aattggatgt
     7561 atgaaaagaa aatcattgtc agacaccgac ctcaacgccg ccagaagact gaaagaaatc
     7621 tggatggcaa agaaatctca gttgggattg actcaggaac gtgcggcgga aattatgggg
     7681 ttttcaacac aaggggcggt aagtcactac ctaaatggtc aaaccccact aaaccttgag
     7741 gctgttctaa agtttgctgg cttactgaaa gttccccctg agtcaatcag gccagacatg
     7801 gtagatttgc tgcaaattgc aaggatgtat tgtcgagaac cacagcaaga taacatagtc
     7861 gcattaccag cagacacaga acaaacagag gatgaactac cgtttgccgt agagccgatg
     7921 gaacgagatt taatccatac gttcagagct tttccgaaag aagatcaaga acacatgctt
     7981 caggagatga aagataaaaa agaatcaatg gatcgtacgg ttgcgcggtg gttagctgca
     8041 caaaaaggcc gtcgcgccta aatcagagga gatatgtaaa atgagtacag tcctttcccc
     8101 aatggtttca gaatttgaga cagttgagca agaaaatagt tacaacgaat ggctgcgggc
     8161 caaagtagca gcaagcctcg cggatccccg cccggcgatt ccgcatgatg aggtaatggc
     8221 ggaaatggaa caaattattg cccaaattgc tgctgcaact cacagaggtg aataatgtta
     8281 cccgttttat ggctaccatc tgctcgtgat gatttgcgcc agatcgtagc ctacatagct
     8341 aaagaaaatc ccgcggcagc acgcagaatg aaaattcgaa ttgaaacatc cgttttgccg
     8401 ctcacagagc atccttacct gtatcctcca agcgagcgag ttcttggtct tcgtgaaatt
     8461 gtaactcatc ctaactacat catcctttac cgagtaacag catcaagcat agaagtagta
     8521 aatattgtgc attctcgaag acagtatcca ggcaaaaaca gttaattatt cccgccgaca
     8581 gaaccacctt agggtggttt tttcttgccc caacaaaaaa caatagcgat gctatttaca
     8641 tcaaaaaaaa taatgatgct attgtttcag gcgtcaaccc accccgcccc acagaatgca
     8701 gggcaatact tcgagttacc agacagtggt caggggttaa gtagccagcc cgaggcgtaa
     8761 gaacatgacg gcagggttca atctttaact atgcagcagg ttttttgttc cgccaccccg
     8821 gcgttaaggg gaaatgaggt cagcatggat actatcgatc ttggcaacaa cgaatctctg
     8881 gtgtgcggcg tgttccctaa ccaggatgga acgtttaccg ccatgacgta taccaaaagc
     8941 aaaacgttta aaaccgaatc tggcgcgcgt tgctggttag ccagaaacac tgactgatga
     9001 ggctgacgat ggaatttaaa gatttaccgt ctgatgtaca gaaaacagca gctcatacat
     9061 tgcattctgt gctgcgagaa atcgggaaag atattgcaag cgagccagca aaagatctgg
     9121 cccggaaaat caagaccgct ttcgttgagc tttataatgt tggcactgac tctgaaactg
     9181 tcgagaccaa gaccgtaagt tcaccaatat tctcacttga gccaagcgta ttatcaggtg
     9241 aaatatgcac cgagatttcc agcgaactgc ttccggtaat acgagaagca attcgccgtc
     9301 gcggtttgga tggaagttat gatcatgacg ttctgcaagt tctcaggaca atggtgactt
     9361 cactggggat ttgatccctg cgtcctctaa gcttttgata tagcggtcct ccaagaagcg
     9421 gtaaattttg tcgaaatcat cttttccctc tggtttattc agaggattac aggacccgta
     9481 agtagcggca tacatttcaa cc
//
LOCUS       DAJTYZ010000143         9478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000143 DAJTYZ010000000
VERSION     DAJTYZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.143"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(383..1360)
                     /gene="epmA"
                     /locus_tag="OO596_003454"
     CDS             complement(383..1360)
                     /gene="epmA"
                     /locus_tag="OO596_003454"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6719228.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1685..3493
                     /gene="frdA"
                     /locus_tag="OO596_003455"
     CDS             1685..3493
                     /gene="frdA"
                     /locus_tag="OO596_003455"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6719229.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3486..4220
                     /gene="frdB"
                     /locus_tag="OO596_003456"
     CDS             3486..4220
                     /gene="frdB"
                     /locus_tag="OO596_003456"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6719230.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4231..4626
                     /gene="frdC"
                     /locus_tag="OO596_003457"
     CDS             4231..4626
                     /gene="frdC"
                     /locus_tag="OO596_003457"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6719231.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4637..4996
                     /gene="frdD"
                     /locus_tag="OO596_003458"
     CDS             4637..4996
                     /gene="frdD"
                     /locus_tag="OO596_003458"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6719232.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5059..6192
                     /locus_tag="OO596_003459"
     CDS             5059..6192
                     /locus_tag="OO596_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6719233.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6281..6814
                     /gene="blc"
                     /locus_tag="OO596_003460"
     CDS             6281..6814
                     /gene="blc"
                     /locus_tag="OO596_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6719234.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="OO596_003461"
     CDS             complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="OO596_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6719235.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7303..7449)
                     /gene="ecnB"
                     /locus_tag="OO596_003462"
     CDS             complement(7303..7449)
                     /gene="ecnB"
                     /locus_tag="OO596_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6719236.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7560..7685)
                     /gene="ecnA"
                     /locus_tag="OO596_003463"
     CDS             complement(7560..7685)
                     /gene="ecnA"
                     /locus_tag="OO596_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6719237.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7737..8303)
                     /gene="efp"
                     /locus_tag="OO596_003464"
     CDS             complement(7737..8303)
                     /gene="efp"
                     /locus_tag="OO596_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6719238.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8345..9373
                     /gene="epmB"
                     /locus_tag="OO596_003465"
     CDS             8345..9373
                     /gene="epmB"
                     /locus_tag="OO596_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6719239.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2399 a         2433 c         2440 g         2206 t
ORIGIN      
        1 aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa
       61 cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc
      121 aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt
      181 tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt
      241 aaaaaccaat acagaattga tacccggatg atatcaaata tccagtctta aattgtggcc
      301 tttaacggaa aaatgaaaac atatgctaca gaatggcgct tatcacgcca ttcttcgctg
      361 ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca
      421 gtgtctccgc gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac
      481 agtcaggcat accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca
      541 aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat
      601 ccgttaattc atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt
      661 cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt
      721 ggtacacaaa ggtcggtttt tctttgccaa tatttggctc tacgccaaag gtaaacagca
      781 attgtagcaa cgtgtcgcgg tcttcttcgg tatcagcaac gttactcaaa tccagtttcg
      841 ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca
      901 agaaagcttg ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga
      961 gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactcaagca
     1021 tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct
     1081 ggaataccgg cccacaaccg gcaaccagca ggcgtttcat atggtattcc gggctggtca
     1141 ttaaccagag attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga
     1201 ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc
     1261 cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcacgtttt aataagttag
     1321 gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg
     1381 acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa
     1441 cagcggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat
     1501 cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac
     1561 ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc
     1621 gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa
     1681 tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg
     1741 tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt
     1801 atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga
     1861 tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga
     1921 gcaggatgtc gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact
     1981 gtgggggtgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg
     2041 catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac
     2101 gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct
     2161 ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaatgaaca tgatggaagg
     2221 cacgctggtg cagatccgtg ctaacgcggt cgttatggct actggcggtg cgggtcgcgt
     2281 ttatcgttac aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag
     2341 ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg
     2401 ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa
     2461 tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa
     2521 aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg
     2581 gcgtaaaggc aacaccatct ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca
     2641 cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta
     2701 cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgaccgcac actacaccat
     2761 gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg
     2821 tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga
     2881 actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg
     2941 taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga
     3001 tctggttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc
     3061 aatggaagaa ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa
     3121 gctggcagag ctgcaggaat gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt
     3181 caacaccgac ctgctctaca ccattgaact gggccacggt ctgaacgttg ctgaatgtat
     3241 ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg
     3301 ttgcaccgag cgtgacgacg tcaacttcct caaacacacc ctcgccttcc gcgatgctga
     3361 tggcacgact cgcctagagt acagcgacgt gaagattact acgctgccgc cagctaaacg
     3421 cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata agaaggagaa
     3481 ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg
     3541 ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg
     3601 atgcgctggt ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc
     3661 gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta
     3721 aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga
     3781 ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac
     3841 cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc
     3901 agatggcaaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt
     3961 gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc
     4021 gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc
     4081 agaacggcgt atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg
     4141 tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg
     4201 cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg
     4261 ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa
     4321 ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct gtttgccctg
     4381 aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg
     4441 atcattaacc tgatcactct ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg
     4501 gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc
     4561 aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac
     4621 tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg
     4681 gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct
     4741 ggtgggtatt ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt
     4801 tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc
     4861 gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc
     4921 tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg
     4981 tatcgttaca atctaacgta tcgccaatgt aaatccggcc cgcctatggc gggccgtttt
     5041 gtatggaaac cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct
     5101 tgctccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg
     5161 cttatagagc aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct
     5221 tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg
     5281 ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt
     5341 gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttactgct
     5401 aaacagtgga atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca
     5461 ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg
     5521 cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc
     5581 ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc
     5641 ttccagccac tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat
     5701 tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct
     5761 gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta
     5821 accccccttg atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc
     5881 tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc
     5941 aatcctgaca gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta
     6001 aaagcgatta agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg
     6061 accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg
     6121 ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac
     6181 gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc
     6241 tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt
     6301 tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg
     6361 cgtgacagta gtgaataatt tcgacgctaa acgctatctc ggtacctggt atgagattgc
     6421 ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat acagtctgcg
     6481 tgatgacggc ggcctgaatg tcatcaataa aggctataac cctgacagag aaatgtggca
     6541 gcagagtgaa gggaaagcgt actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc
     6601 attctttggt cctttctatg gcggttataa cgtcattgca cttgatcggg aataccgcca
     6661 tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat
     6721 ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag
     6781 taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat
     6841 ccccaatacg attaacgcca gactcgccag gcgcatcggg ttagcggact caccgagcag
     6901 cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt
     6961 ccctaccggt aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt
     7021 cacagtaata acacttggcg tcaaacgact aaagccgtgg gtatatttca ggccaacggc
     7081 ccataccact tccagcagac cagcaataac taagataatc caggacatat caggctccgg
     7141 agaattgggg ccatccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg
     7201 ttgaacggat attttgccag gtggcagggg aatttcaact attacctttg tgactatcgc
     7261 aaaaaaacaa acggcacgac ataactgtcg taccgttatt gcttattgct gcgctttcgt
     7321 tgctgcacca gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt
     7381 gttgcaggca gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt
     7441 cttcaccata acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat
     7501 aatagacaac atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat
     7561 tagctggcag cgcgggagat ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga
     7621 gcggtgttac agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc
     7681 atcatctctg acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac
     7741 ttcacgcgag agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt
     7801 acgaacagcg gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta
     7861 cctgcggtat cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc
     7921 ggcggagtaa cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc
     7981 agcagccatt tagcgttgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt
     8041 tcgttgttca tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg
     8101 acaacatcag cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg
     8161 gtcagcagac gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat
     8221 tcactcgctt caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa
     8281 tcgttgctat agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca
     8341 caaaatggcg catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact
     8401 tgccgatgtt gtgaccgatc ctggtgaact tctgcgtctt ttgaatatag acgcggacga
     8461 aaaactgttg gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt
     8521 tatcgatcgc atggagaaag gcaatccgga cgatcctatt ttgcgtcagg tacttacctc
     8581 gcaagatgag tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag
     8641 cgtagtgcct ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg
     8701 ctgcgcggta aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg
     8761 caacaagcgt aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga
     8821 gatgattttc tccggcggcg atccgctgat ggcgaaagat cacgagctgg actggttgct
     8881 cacacaactg gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat
     8941 tgtgatcccg gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca
     9001 aatcttgctg gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc
     9061 gatggctaaa ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg
     9121 tgtgaacgat aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt
     9181 gatgccgtat tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag
     9241 tgatgacgaa gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt
     9301 gccgaaactg gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg atctccagct
     9361 acgccagcag taactgatta aacagggaag ttaaacatta gcctgatgcg ctacgcttat
     9421 caggcctaca ttgtgcattg aaattgaatg gatttgcaat gaactgtagg ccggataa
//
LOCUS       DAJTYZ010000144         9432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000144 DAJTYZ010000000
VERSION     DAJTYZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.144"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            225..2201
                     /locus_tag="OO596_003466"
                     /pseudo
     CDS             225..2201
                     /locus_tag="OO596_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2194..3330
                     /locus_tag="OO596_003467"
     CDS             2194..3330
                     /locus_tag="OO596_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6719240.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3327..5327
                     /locus_tag="OO596_003468"
     CDS             3327..5327
                     /locus_tag="OO596_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR6719241.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5449..5910
                     /locus_tag="OO596_003469"
     CDS             5449..5910
                     /locus_tag="OO596_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6719242.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5951..6421)
                     /locus_tag="OO596_003470"
     CDS             complement(5951..6421)
                     /locus_tag="OO596_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6719243.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6468..7187)
                     /gene="yehT"
                     /locus_tag="OO596_003471"
     CDS             complement(6468..7187)
                     /gene="yehT"
                     /locus_tag="OO596_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6719244.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7184..8869)
                     /gene="btsS"
                     /locus_tag="OO596_003472"
     CDS             complement(7184..8869)
                     /gene="btsS"
                     /locus_tag="OO596_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6719245.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9091..9156
                     /locus_tag="OO596_003473"
                     /pseudo
     CDS             9091..9156
                     /locus_tag="OO596_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT         2298 a         2493 c         2418 g         2223 t
ORIGIN      
        1 agcggccacg atacgtattg attgaaggcc cggctgattt taatgaccgg gtagacgaac
       61 tgtttttagc ccaccagctt ccggtagcta tttacagtta ttgccagtat caggacggtg
      121 cagcccccgg gcgtggtgcc tggacgccat ttgctgaatt ttcgccggag tggcaggcgc
      181 tacaagccgc acgtcgtatt caggcacaaa cttacttcat cgatttgcct tgctgggcgc
      241 agagtgaaga agtggacgat tcgcctgata cgcaagagga aagccaggcc ttgctgctgc
      301 gtgccacccg catggataac agcgacaccc tgtgggatca cttgttcgaa gatgaaagcc
      361 agcaaactgc attaccctct gcactggcgc actattttgc ccaactgcgg ggcgattccc
      421 ccggcgatgc actcaatcgt cagcgcgaag cctttatggc ccgctggatt ggatgggcga
      481 tgcagcaaaa caatggcgac gtgttagtcg tctgcggtgg ctggcacgct cctgcactgg
      541 caaaaatgtg acgcgaatgc ccgcaggaca ttaacacgcc ggaattgccc tcgctggcag
      601 atgccgttac aggttgttat ctcaccccct acagtgaaaa gcgccttgat gtgctggcag
      661 gatacctttc cggtatgcct gccccggtct ggcaaagctg gtactggcag tggggcttac
      721 agcaggcggg agaacaactg ctaaaaacgg ttcttacccg tttgcgccag cacaaattgc
      781 ctgcttcgac cgcggatatg gctgccgctc atctgcatgc gatggcgctg gcacagttgc
      841 gcggacataa actaccgtta cgcactgact ggctggatgc catagcaggc tcgctgatta
      901 aagaagccct gaacgcgccg ttgccgtgga gctatcgcgg agttattcat cccgacaccg
      961 atccgattct gctaacgttg atagacacat tagcgggtga cggattcggt aaacttgccc
     1021 cttctacgcc acaaccgcct ctgccaaaag atgtcacctg cgaactggaa cgtaccgcaa
     1081 tctctcttcc ggcggagctt accttaaatc gctttaaccc caatgggcta gcgcaaagtc
     1141 aggtgttaca tcggctggca atactggaga tcccagggat tgtacgccag cagggaagca
     1201 cactgacact tgcaggcaac ggtgaagaac gctggaaatt aacccgcccg cttagccagc
     1261 atgcggcatt gattgaggcc gcctgctttg gtgccacact ccaggaagcc gcacgccata
     1321 aattagaagc cgatatgctg gacgcgggtg gaatcggcag tatcaccaca tgtcttagcc
     1381 aggcggcgtt agcgggtctg gcgtccttca gtcaacaatt actggagcaa ctcacattat
     1441 taatcgccca ggaaaatcaa tttgccgaaa tgggccaggc gctggaagtg ctatatgcct
     1501 tatggcggct ggatgaaatt agcggtatgc aaggcgcgca gatattacaa acgacgttat
     1561 gcgcggctat cgatcgcacg ctgtggctgt gtgaatctaa cggcagacca gatgaaaagg
     1621 agtttcacgc tcacctgcat agctggcagg cgctttgcca tatcctgcgc gatctacata
     1681 gcggcgttaa tttacccggc gtttttcttt ctgcggcggt agccttactg gagcgccgca
     1741 gtcaggcgat tcatgtcccg gcgctggatc gcggcgcggc tcttggcgca ctgatgcgtc
     1801 tggaacatcc caacgccagt gccgaagcgg cgctgacgat gctggcgcag ttatccccgg
     1861 cacaatccgg cgaggcgctg cacggtttgc tggcgctcgc ccgtcatcaa ctggcctgcc
     1921 aaccagcatt tatcgccggt ttcagtagtc atttaaatca actgagtgac gacgatttta
     1981 ttaacgccct gcccgatttg cgcgcggcga tggcctggct accgccacga gaacgcggga
     2041 cactggcgca tcaggtgctt gagcattacc agcttgcgca gcttcctgtt tcagcgctgc
     2101 aaatgccgtt gcattgtcct ccgcaagcca ttgcacatca tcaacaactc gaacagcagg
     2161 cactggcatc gctgcaaaac tggggagttt tccatgtctg aactgaacga tcttctgacc
     2221 acccgtgagc tacaacgctg gcgattaatt cttggcgaag cggcagaaac gacgctttgt
     2281 gggctggatg acaacgcccg gcagatagac cacgcgctgg aatggctgta tgggcgcgat
     2341 cctgaacggc tccagcgtgg tgaacgctcc ggtggattag gtggctcaaa tctcaccact
     2401 cctgagtgga tcaacagtat tcacacgctg tttccgcaac aggtgattga gcggctggaa
     2461 agcgatgccg tactgcgcta cggcattgaa gatgtggtga cgaatctcga catgctggaa
     2521 cgtatgcagc cttctgaaag cctgctacgc gccgttttgc acaccaaaca tctgatgaat
     2581 cccgaagtac tggctgccgc ccgccggata gtgcgccagg ttgttgaaga aattatggct
     2641 cgactggcaa aggaagttcg tcaggctttt tctggtgtcc gcgatcgccg tcgccgctca
     2701 tttattccac tggcgcgaaa ctttgatttc aaaagtactc tgcgcgctaa cctgcaacac
     2761 tggcacccgc aacacggcaa gttgtatatc gaatcccccc gctttaacag ccgcattaaa
     2821 cgccaaagcg aacaatggca actggtctta ctggttgatc aaagcggatc gatggtggac
     2881 tcggtgatcc actctgcggt gatggcggcc tgtttgtggc agttacccgg cattcgtacc
     2941 catctggtgg cgtttgacac aagcgtggtt gatctcacgg cagacgttgc cgatccggta
     3001 gagttattaa tgaaagtaca gttgggcggc gggaccaata tcgccagtgc cgtggagtat
     3061 ggtcggcaac ttattgaaca accagcaaaa agcgtcatta tcctcgtgag cgatttttac
     3121 gaagggggtt catcatcatt gctgacgcat caggtgaaaa agtgtgtcca gagcggcatc
     3181 aaagtgctgg gactggctgc gctcgatagc accgcaacac cttgctatga ccgcgatacg
     3241 gcccaggcgc tggttaatgt cggcgcacaa atagccgcca tgacgccggg cgagctggca
     3301 tcatggcttg cggagaatct tcagtcatga attcactacg tccggaatta ttagaactga
     3361 caccgcaggc cctgacggcg ttaagcaatg ccggttttgt taagcgcagt cttaaggaac
     3421 tggaaaatgg caacgtcccg gagatcagcc atgagaacga cgctttaatc gccaccttca
     3481 gtgacggtgt ccgtacccag ctggcgaacg gccaggcact gaaagaggct cagtgcagtt
     3541 gcggggccaa cggtatgtgc cgtcatcgcg tgatgctggt gttaagttat caacgacttt
     3601 gtgccaccac tcagtctacg gaaaaagaag aagagtggga tccggcaatc tggctggaag
     3661 aactggctac ccttcccgat gctacccgca aacgcgcaca ggcgctggtc gctaaaggca
     3721 tcaccattga gttgttctgt gcgccgggtg aaattccctc tgcccgctta ccgatgagcg
     3781 atatgcgttt ttattcccgc agcagtattc gtttcgcccg ttgtgattgt attgaaagca
     3841 cactttgcga acatgtcgta ctggcagtac aggcctttgt ccaggcaaaa gcgcagcaag
     3901 cggaattgac tcatttaatc tggcagatgc gcagcgaaca cgtcacatca tctgacgatc
     3961 cgtttgccag cgaagaaggc aaaacgtgtc gtcaatatgt tcaacaacta agccaggcat
     4021 tatggctgag cggcatcagc cagccgctta ttcactacga ggccgctttc agccgcgcgc
     4081 agcaagcagc ggaacgctgc agctggcgat gggtgagtga atcgctacgg caactgcgcg
     4141 ccagcgttga tgccttccac gcccgcgcca gccactatca tgccggagaa tgcttacgtc
     4201 agcttgcggc attaaacagt cgattaaatt gcgcacaaga gatggcccgg cgcgacagta
     4261 ttggtgaagt tcctcctgtg ccgtggcgca cggtcgttgg ctctggcatt gccggagaag
     4321 caaagcttga tcatctgcgg ctggtgtctt taggtatgcg ttgctggcag gatattgagc
     4381 attatggttt acgcatctgg tttaccgatc ccgacaccgg cagtattttg cacctttcgc
     4441 gcagttggcc gcgaagtgaa caggaaaact caccggcagc tacgcgtcgg ctgtttagtt
     4501 ttcaggctgg cgcactagcg ggcgggcaaa ttgtttcaca agcagcaaaa cgcagtgccg
     4561 atggcgagct gctgttagct acccgcaacc gcttaagcag cgttgtgccg ctgtcgcctg
     4621 atgcctggca aatgttgagc gcgccgttac gccagccggg cattgtggct ttgcgggaat
     4681 atttacgcca gcgtcccccc gcctgcatac ggcctcttaa tcaggtcgat aacttattta
     4741 ttctgccggt cgctgagtgt atttcgctcg gttgggatag cagccgccag acgctggatg
     4801 cgcaggtaat cagcggcgaa ggtgaagata atctcctgac gttatccctt ccggcatctg
     4861 cctgttctcc ttttgccgtt gaacgcatgg cggcgctttt gcaacaaaca gacgaccccg
     4921 tgagtctggt ttctggcttt gtcagctttg tagaggggca attgacactg gaaccacggg
     4981 tgatgatgac aaaaacccgc gcctgggcgc tggacgcaga aactgcgcct gtggcaccgc
     5041 taccttctgc cagcgttttg cctgtgccgt ctaccgctca tcagttgctg atgcgctgcc
     5101 aggcgttact tattcaactg ctccataacg gctggcgcta tcaggaacag agcgctatta
     5161 gtcaggcaga gttgctggcg aatgacctct ccgcggtcgg tttttatcgg ctggcacatg
     5221 tgttgggaca atttcgtaat acagaaagcg aggcacgggt agaagcaatg aataacggtg
     5281 ttttactttg cgaacaatta ttccccatgc ttcagcaaca aggatgaaat agtgcttact
     5341 aagagttcta ctccagttcc ggactgctca cgccacggta ttaggcatat cctatatagc
     5401 ccctggtgag agtcaccagt tccttgatta aataaaatgg agttttacat gaaggctttc
     5461 aataagctgt tttccctcgt tgttgcatct gttctggttt tctctcttgc tggctgcggt
     5521 gacaaagaag aatcgaagaa attcagcgcc aatctgaacg gcactgaaat tgccattacc
     5581 tatgtctaca aaggtgacaa ggtgcttaag caatcttctg aaaccaaaat tcaatttgcc
     5641 tccattggtg caaccaccaa agaagatgct gccaagacac ttgagccgtt aagcgccaaa
     5701 tacaaaaaca tcgcgggtgt tgaagaaaaa ttaacctata ccgataccta cgcgcaggaa
     5761 aacgtgacta tcgatatgga aaaagtggat tttaaagccc tgcagggtat ttcaggaatc
     5821 aacgtttctg ctgaagatgc caaaaaaggt atcactatgg cgcaaatgga actggtgatg
     5881 aaagccgctg gttttaaaga agtgaaataa tctgtcggcg gccatgtttc gcatggccgc
     5941 catacccgtc ttagcttttc ttcacatgct ggcgcgctgc cagtccacgc agaaaataac
     6001 gtaaaaattg atcgccgcat tcgcggaagt ttttatgatc cggtgcgcgc atcatcgctg
     6061 taatttccgg catcgaaacg cggaactgct gttcggtgag gatcgccaga atgtcatcgg
     6121 ttttcagcga aaacgcgatg cgtaattttt tcagcacgat gttgttatta atgcgacgtt
     6181 ccggctccag tgccggagca gactcatcct tgccgcgttt ttcataaatc aggccattga
     6241 ggaatgacga caaaacaatg tccggacaac gctgaaaacc ctcttcgtct tctttacgta
     6301 gccagacggc gatctgttcc gcggtggctt cgacattacc cagcgccaga atacgcacca
     6361 ggtcattatt attggctttc aaaatgtagc gcacgctgcg cagaatatcg ttacttagca
     6421 tgaggccttc aggtgttaat gaggcaaaaa gccattttag cagtctttta caggccaatc
     6481 gcctctttta agcttttcag ataacggcgg ctgaccggca cggttaagcc attacgcaaa
     6541 atcaactcgg cctggccgtt atcttccaga cgaatctcct gtaaatgcgc gaggttaacc
     6601 agatactgac gatggcagcg cagtagtggt gtacgacttt ccagggtacg taatgtcaat
     6661 tcggtaaagc cctctttccc ttcgtggctg gtaacgtaga caccgctcat ccgactgctg
     6721 acaaatgcca catctttcat ttgcagcaaa taaatccgac tatgccccgt acaagggata
     6781 aatttcagcg cctgttgatt ttccggtaac agcgaaacat cctgcttgct gcgctcctga
     6841 cgcaatcgcg ccagcgtttt ctccagtcgc gcttcatcaa ttggcttcag cagataatca
     6901 aaggcatgtt cttcaaaggc tttaatagcg tattcgtcaa acgcagtgag aaaaacaata
     6961 tacgggcgat gttccgggtc aagcatcccc accatttcca gaccactgat gcgcggcatc
     7021 tggatatcga gaaacagcac atccgggcgc agtttatgca ccgcgccgat cccttccacg
     7081 gcgtttgaac actctccaac gatttcaata tcgctctgct cctgcaaaaa tacacgcagg
     7141 ttctcccgtg ctaacggttc atcatcgaca attaagactt taatcatgcc tcgtccctcc
     7201 atggtagtcg taacgttatt cgggtgtaac tatcaggctc acaggcgacg cttattccat
     7261 agtcatcgcc aaaccgttca cgtaaacgct tatccaccag attcatcccc agcccactgg
     7321 cattggttac cggttgatac aaaccggcat tgtcttcgat ctccagcatc aaatgttgcc
     7381 cctcacgtcg ggcgctgatt gccactcgcc ctgtatccag cagttgtgat gtcccatgtt
     7441 taatggcgtt ttccactatc ggttgcaggg taaacgcggg caattgctgc tgggataatt
     7501 cttgcggaat agcaatgttg acctgcaacc gcgactggaa gcgcgccttt tcaatttgca
     7561 gataagcgtt cacatgttca atttcgtcgg cgagagtaac aaactccgaa ggccgcttta
     7621 agtttttgcg gaaaaaagtg gaaagatact gcaccagctg gctggcctgt tcgctgtcgc
     7681 ggcggatcac cgctttaatg gtgttaagcg cattaaacaa aaaatgagga ttcacctggg
     7741 cgtgaagcag tttgatttct gactgggtga gcatcgcttt ttgccgctca tattgcccgg
     7801 caagaatctg cgccgaaagc agttgcgcaa tcccctcgcc cagcgtgcgg tttattgaac
     7861 tgaataaacg gtttttggct tcatacaatt tgatggtgcc catcacgcgc tgattttcgc
     7921 cacgcaacgg aattaccagc gtcgacccca gtttgcattg cggatgcaaa gagcaacgat
     7981 aaggtacttc gttgccatca gcgtagacca cttcaccggt ttcaatcgct tttaaggtgt
     8041 aaggcgaaga aatcggtttg ccgggtaaat ggtggtcgtc accaattccg gtaaaggcca
     8101 gcaatttctc tcgatcggta atcgcgactg caccaatatc cagctcctga tacagcacct
     8161 gagccacttt catgctgttc acttcgttaa acccctgtcg caaaatgcct tccgtcgagg
     8221 ctgccacttt cagcgcagtg gcagaaaaag ccgaagtgta tttttcaaac atcgcgcgtt
     8281 tatcgagcaa tatacgcata aacagcgccg cgccgacggt attggtgacc atcattggcg
     8341 cagcaatatt actcaccaga cgcaccgcat cttcataagg tcgggcgatc gccaggatga
     8401 tcagcatttg caccatttca gcgacgaacg tgacggcacc ggcggtaatg gggttaaaga
     8461 ctttatcagt gcgcccgcga cggatcagga tgctgtgtac caggccgccg agcaatcctt
     8521 caacgatggt tgagatcatg caacttagcg cggtcatgcc ccccatcgaa tatcgatgta
     8581 agccgccggt cagaccaacc agcccaccga cgaccggacc gccgagtaag ccgcccatta
     8641 ccgcgcctat cgcacgggta ttggcaatag aatcgtcaat gtgcaaccca aaccaggtgc
     8701 ccatgatgca gaagatggaa aagacgatgt agcagagaaa tttatgcggc agacgaaccg
     8761 tgacctgcat taacggtatg aataatggcg ttttactcat taaccacgca atgactaaaa
     8821 aaacgcacat ctgctgaagc agcagcaaca ccagattaaa atcgtacata cccgcaaacc
     8881 acacttccct ttaaaacgcg taacatacat tgcctgcgtt taactttctt tgaactcttg
     8941 cagaaaaatg agaattcgtg agtacgatca ctcaaaatcg cctggcaaaa ataaaatcac
     9001 cctatagatg cacaaaaaac gggcaaaact acctggttcg caaaactgcg tctaaagtta
     9061 aaccgggacc tcgcgagcaa gggtgagacg atggcgcttt acacaattgg tgaagtggcg
     9121 ttgctttgtg atattaaccc tgtcacgtta cgcgcgtgac gcaacatttt taaaaatcga
     9181 cgaagatcgt ccaggagcgc cgtttttcaa gggttggata gatgattcgt ttaccatgtc
     9241 gacgaacttc gacgaatttc gccgataaaa agtgtcccat acatgcccca ttgatgcccc
     9301 ataaacaata tcaggaattg acactggtta tccatacaga taaaaataac acctgtacat
     9361 aaccacagtt acaggagggt gttatgcgtg ttgagatctg tatcgctaaa gaaaaaatca
     9421 ctaaaatgcc aa
//
LOCUS       DAJTYZ010000145         9428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000145 DAJTYZ010000000
VERSION     DAJTYZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.145"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..514)
                     /locus_tag="OO596_003474"
     CDS             complement(<1..514)
                     /locus_tag="OO596_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6719246.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNP"
     gene            complement(786..4688)
                     /gene="hrpA"
                     /locus_tag="OO596_003475"
     CDS             complement(786..4688)
                     /gene="hrpA"
                     /locus_tag="OO596_003475"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6719247.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4889..5494
                     /gene="azoR"
                     /locus_tag="OO596_003476"
     CDS             4889..5494
                     /gene="azoR"
                     /locus_tag="OO596_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6719248.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5545..6861)
                     /locus_tag="OO596_003477"
     CDS             complement(5545..6861)
                     /locus_tag="OO596_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR6719249.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(6851..8608)
                     /locus_tag="OO596_003478"
     CDS             complement(6851..8608)
                     /locus_tag="OO596_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6719250.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8624..9428)
                     /locus_tag="OO596_003479"
                     /pseudo
     CDS             complement(8624..9428)
                     /locus_tag="OO596_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2384 a         2576 c         2226 g         2242 t
ORIGIN      
        1 cgggattgtc cccagtcata cggcggaacg tcgccatcgt gcgccgctgc atggtggggt
       61 cctgaacaac gatagccgta tgaactcgtt ctacggcctg attcaatagc gcgatgctaa
      121 agcgtgcgtt ttcaccgcag tttgttgact ggtcttcaat ccagattttt tcatgcggaa
      181 tgtgccagaa ctgatgagcg atatccgcct ggatggtcgc ttctgctctg ccagtggtgc
      241 ggatagtgtt gtagtgcgga tgctgtgcga tggcgctata caaaaaagtt gtcgagtgac
      301 cgataccacc actaatcagt aaaggaattt gctgatcgcg agcaatctta catgccgcgt
      361 cgatagtcgg cataaccgca ttgcctgcaa ggatcacgca atcggcctga tacggcacct
      421 caccggagaa atcatcctgc gccagccact gtccgataac atttatggca tctatagttg
      481 ccggcgaaag cgtcggaaac ggggtaatgt tcatggttcc tccttatcgt tcccttaaga
      541 atactgcgtg agataatagt gagaagcgga gcactctgaa aggaggaagt cgtatttcct
      601 taaccgccac tccgcaagcc tgacatatct tttaaggaaa tgctgtagat aaacattatt
      661 catcttgcag cagagaattg tatttttctg aattcctgaa tttttaccct taccgtttta
      721 gaattaataa aaaacccggc gctaaatgct taccgggttt tttctttatc aggcaaatag
      781 cagggttaac cgctaatctg ctccatcgcc tgcaaaatac gcttatctga aatcggataa
      841 ggcgtaccaa gttgttgagc gaagtaacta acacgcaact cttctatcat ccaacggatc
      901 tctttcacgt cttcatcctc acgacgtgca ggcggcagtt tgttgatcca ttgctgccac
      961 gcctgctgga cgttttcgac tttcagcatc tgagcacggt cgcgatgtgg atcaaccgcc
     1021 agtttttcca gccgtttttc aatcgcctgc aaatatcgca gcgtgtcgcc cagccgtttg
     1081 aagccgttac cagtgacaaa accgcgatat accagcccgc ccatctgcgc tttaatgtca
     1141 gaaagcccca gcgccatcgt catatccacc cgccccttca gacgtttgtt gatattgaac
     1201 actgccgtaa ggatttgctc gacctgcttc gcaatatcca ccaccgtgtc gttcagttcg
     1261 gcacgcactt tttcatgcag cgcagcaaag ccttcttccg tccagaccgg gccaccattg
     1321 gcgtcgatca atttatccac accgcaggag atacagtcgt cgatcagctc cagcactttg
     1381 ccatacgggt taaagtacag ccccagcttg gctttgtttg gtaacttctc atgtaaatac
     1441 ttgatcggcg agggaatatt cagcagcagt agacggcgaa gaccgttcca cattacctgt
     1501 ttttgctcca gcggattatc aaacagtttg atcgccacac tgtcgcgttc atccactagc
     1561 gctggccacg ccttcacttt gtagttgcca cgcttctgtt cgtagctttc cggcagctga
     1621 ccaaaactcc agatatgcag tccgctctgc tcgataccgt catccgccac cgcagacagc
     1681 gtttcctgca ctttgccttt cagcgcatct ttcagatctt gtagcgagcg cccttctttt
     1741 agcttcttgt ttttgtcatc caccacgcgg aaggtgattt tcaggtgatc gggcacctga
     1801 tcccagtgcc agtcttcgcg gtcaacggta acgccggtca tccgccgtaa ctcgcgctca
     1861 aggctgtcga gcaacggtaa ctccagcggt gtgacgcggc ctaaaaacgc ttcggcgtag
     1921 tttggtgcag gcacaaaatt acggcgtacc ggtttcggca acgatttaat cagagcaatc
     1981 accaattcgc ggcgcagacc ggggatctgc cattcaaacc cgctttcctc aacctggtta
     2041 agtaacggca gcggaatatg tacggtcaca ccgtccgcgt ccgcgcctgg ctcaaactga
     2101 tagctcaaac gcagcttgag attgccctga tgccagaagt tcgggtaatc cagctttctg
     2161 attttttccg ccccttcttt gatcaacatg ctcttttcaa agttgagcaa atcaggcgtt
     2221 tcgcggctga cttttttcca ccagctatcg aagtgacgag cggagattac atcgtggctg
     2281 atgcgctggt cgtagaactc aaacagcgtt tcgtcatcaa ccagaatatc gcggcgacgt
     2341 gatttgtgtt ccagctcttc cacttcggcc cgcagtttca ggttttcacg gaagaatgcg
     2401 tgacgcgtct gccagtcacc ttccaccaga gcgtggcgaa taaagagttc acgacataac
     2461 gccgaatcga tctggctgta gttgaccttg cgcgcagcaa caatcggcaa accataaaca
     2521 gtgacttttt ccgttgccat caccgcgccc tgcgcccgtt cccagtgcgg ttcgctgtag
     2581 gtgcgtttaa tcaaatgctg agcaacgggt tctacccatt ccgggtcgat acgcgcagca
     2641 atgcgccccc acaggcggct ggtttctacc agttccgcca ccattaccca tttcggcggc
     2701 tttttgaata aaccagaacc ggggaagatg gagaaacgcg cgttacgtgc accggtatat
     2761 tcctgtttat cggcatcttt catgccgata tgggaaagca aaccggtcag taaggcgatg
     2821 tgaatttcac gatactccgc cggttcgctg ttgaccggaa tgccaagttc tttcaccacc
     2881 tggcgcaact gggtgtagat atcctgccat tcgcgcacgc gcagatagtt gaggtaatcg
     2941 gtacgacaca ggcgacggaa ggcgttggaa gaaagcgcct tttgctgctc gccaagataa
     3001 ttccacagat ttacaaacgc gaggaagtcg gattctttgt cgtggaagcg acgatgtttt
     3061 tcgtccgatg cctgctgttt gtccatcgga cgttcgcgcg gatcctgaat ggagagcgcg
     3121 gacgtgataa tcatcgcctc acgcacgcag ccatgttttt gcgcttccag caccatacgc
     3181 gccagacgtg ggtcgacagg caactgcgag agctggcgac cgagcggcgt cagtttatag
     3241 gcgctggcct gttcatccgt ggtgatcgcg cccagttctt cgagcagacg cacgccatcc
     3301 tggatattgc gtttatccgg tgcttcgaca aacgggaacg cagcgatatc gcccagcccc
     3361 agcgcggtca tctgcaaaat aaccgaggcc aggttggtac gcagaatctc cggatcggta
     3421 aactccgggc gcgagaggaa atcgtcttcg gaatagagac gaatacagat cccttcggac
     3481 acacgaccac agcggccttt acgctgattg gcagacgcct gcgaaatggg ttcaatcggc
     3541 aaacgctgca ctttggtgcg atagctgtag cggctgatac gcgctgtacc ggggtcgatg
     3601 acgtatttaa tccccggtac ggtcagcgag gtttccgcga cgttggtcgc cagcacaatg
     3661 cgccgtccgc tgtgcgactg gaacacgcgg ttctgttcgc tgttcgaaag ccgcgcataa
     3721 agcggcaaga tttcggtatg gcgtaagttc agcttgttca gcgcatcggc ggtatcgcga
     3781 atttcccgct caccactcat aaagatcaga atgtcgccag ggctttcctg actcagttcg
     3841 tctacggcgt caaaaatcgc ctgcaactga tcgcgctcgg tgtcatcggc ttcttcaaca
     3901 atcggtcgat agcgcacttc caccggatag gtccgaccgg agacttcgat aatcggcgca
     3961 ttattaaagt ggcgcgaaaa gcgttccggg tcgatagtcg cggaagtgat aatgattttt
     4021 aggtcaggac gccgcggcag caactctttc aaatagccga gcagaaaatc gatattcagg
     4081 ctgcgttcgt gcgcttcgtc aataatgatg gtgtcgtact gcatcagcag gcggtcttgc
     4141 tggatctccg ccagcaggat accgtcggtc atcagcttga ccatcgtgtt atcacttacg
     4201 tgatcgctga aacgcacttt gtaaccgatg caaccgcccg gctccgtttt cagctcttcc
     4261 gcaatacggt tcgccaccgt tcttgccgcc aggcgacgcg gctgggtatg gccgatcagc
     4321 cctttaatcc cgcgccccag ctccatacag attttcggta actgagtcgt tttaccagaa
     4381 cccgtttccc cggcgacgat caccacctgg tgatcacgaa tcgcttcgag aatgtcctgt
     4441 tttttctgac taaccggtaa attgtcagga taagtaattt ccggtcgtgc cgcttcacgc
     4501 agcaggactt tccctgccgc ctggtcaatc tctttcgcca tctcctggaa aatggcctgt
     4561 tgtgcatcag gatttttaac cttcttcacg ccgtgcagac ggcgagaaaa acgcagtctg
     4621 tcacgcagca tcagcgaatc cagccgctgc tgcaaggccg taaaggtcaa tttttgttgt
     4681 tctgtcataa cgttagtggg cagtagtctg cctgattaat tttctttgaa atgatagata
     4741 tagagtacca catcacggct ttctatgcgt tgttcaataa attcgaacat aggcttcgat
     4801 ttattgcgct atctctgtgg caggattgtg aataaagtgt caacaagcaa cggggcatcg
     4861 cccattcaaa catctataag gaaacaccat gagcaaggta ttagttctta aatccagcat
     4921 cctggcaggg tactctcagt ctaatcagtt gtccgattat tttgttgaac aatggcgcga
     4981 aaagcactcc gctgatgaaa tcaccgttcg cgacctggct gcaaatccga ttccggtact
     5041 ggatggcgaa ctggttggtg cactgcgtcc gagcgatgcg ccgctgactc cgcgtcagca
     5101 ggaagctctg gcgctttccg atgagctgat tgccgagctg aaagcccacg acgttatcgt
     5161 tattgcggca ccgatgtata acttcaacat ctcaactcag ttgaaaaact attttgacct
     5221 ggttgcacgc gcaggcgtta ctttccgcta taccgagaac ggtccggaag gtctggtaac
     5281 gggtaaaaaa gccatcgtta ttaccagccg cggtgggatc cacaaagatg gaccaacgga
     5341 cctggtgacg ccgtatctgt ccacgttcct cggctttatc ggcattactg atgtgaaatt
     5401 tgtcttcgcc gaagggatcg catacggtcc ggaaatggca gcgaaagcgc agtctgacgc
     5461 gaaagcagcc atcgacagca ttgttgctgc ataaccggac acgatcttga tgtggactgt
     5521 agtccagtcc acatcccaat ccacttacct gttttcccat aatttcagca tcgctttgtg
     5581 atcttcctta agcacaatat gcgagcgtct ggctcgaata aagctaatcg cttcatcaac
     5641 ggttttacag tatccgtaac ataacagcca tgccgccact accagcgcac tgcgcgataa
     5701 tcccagtgcg caatggacca gaacgctgcc ttgctcttcg cgtaatgttt ccagcatcgc
     5761 cacggcctgt cggagctccc cctcttccgg aaccaccaga tctagcatcg gtacacagaa
     5821 atagagtcga tcttttgtcg ctcgtccacg agggaattca aaggtgacgt ccagaaccgc
     5881 attctgtgcc ggaatatgtc gtggaaacgc ccctaaataa acaccagcag tcattttgct
     5941 caccggctcc aggcgacgac aaaaccaacg catagatagc cacatcccga tgcgccaggg
     6001 caatgtcagc cagtaaacgg cgggcgggag tttcccctga ctatctttcc ctgttgttat
     6061 cgcgccaagc ccaccgtacc cacggccaat aatgagtagc gataatactg gccaacataa
     6121 ccagactgac caccataact gaaccatcat cattagctcc atcaacacaa tgcacgcgca
     6181 cgcgcctacg acatatggca gtgctatttt gattcgacgt tgatcaggtt tctgataatt
     6241 ccaacgacgg tcgacgggca ccatccagtc aatcaacata cctaccgcca gccctgtgat
     6301 gacatcaata aaatgatgct gccatgtcgt cagcgtcgaa atggcgatga gtaaaaacca
     6361 tccgccgcag actttacgcc acctcacagc cagatgctga cgaaagtgac gccaaagtag
     6421 ccagcagaga ataatatgca gcgaaggaga ctggttataa ggcagatcaa acagttcaag
     6481 ttgcgaaaat agccatcccg tcaccccact cacttcagga cggataaaac taaacttcag
     6541 cggatagagc aaaaaaccgc agcaggccat taccgttgcc agaataagcc ggtggacaag
     6601 tcggcgctgt tcgaatgtcg tgctacaaac gaacagcgaa aatccatata aaagatccag
     6661 actccagtaa ggaacaatag tccagggaag aaaagggatc gccgtttccc aaccgaatac
     6721 ctgactggga atatcatggc tattactgtc ctgaaccgcg gtgaactgat taagagatcc
     6781 ataggtgaag aagaaaaacg gggccagcaa caataaccag ccagctcctt gtagcaatac
     6841 gttccggcgt tcagttatca cgacgcaccg ccatcgaaac cgtaaaaatg ccccactcat
     6901 caatccgttg tgtgcattta tcaaatccag catcatgcac gagcaaatcc atctcccctt
     6961 gcgtacgcac gcgcattacc cacagtttac catctttatg actggttaac accccggcaa
     7021 tcagctccag ttgtgggtgc cacggctgcc cggtgtagat gagaatgcca cccggttcga
     7081 tggcattggc aagacctgcg agtgagtttt ttacctgctc attttcggga aacagctcat
     7141 acagtccaga gacaatcgcc agcgtaggcc gcggagttaa cgcgctgagt tcctccgggt
     7201 taaacgcatc gccctgttca aaacgcaccc gcccagacat tccccgttgg gcaatcatct
     7261 cttgcccctg tgcaacattt aactcactgt aatcacgtaa taaaatatcg cttacggcag
     7321 gctcgtttgc cagtgcatcc agtacatagc gcccatgccc tgcggcaatg tcaaccacgc
     7381 ggacggctaa acctttgacg tggagatcgg caacggcttg tttaatcagt atttgtaaat
     7441 gagttttgcg ctggcgaata ccgcgccagc caacactgtt caggtaaatt ttgtcgatta
     7501 agcgcccgaa tgcgttactg ccttgcggtt gattttgata gacataatcc agcgaactgc
     7561 ctgaatcaaa gccagtgctc attccgagat gcaggcccgc agagtgcgtc ccgaacaatt
     7621 tcatcgcttt acgcataaag cgatacgcca aatcaaccgg cgataatggc acgggtccac
     7681 ctgaaaggag gcgccagcga tccgctgatg gtccagtgcg gtcttcatgc tgataatcaa
     7741 atttttgtga cttgttagca tataaacgac gaataaagct ttgcattttt tcaaatgcct
     7801 gcgccctgtt ctcttcaccc aacgtgtcgt gataaaagcc tggcagcaga tgcagctctt
     7861 tcagagggct acgtaatctc tgataaaaat caatctgcgg ttggcgatgc accacatagt
     7921 catcgcctga tatcagaagt tgcgtgggga gcgtaatcgc cgccgcatct ctaataatac
     7981 gttcagacgt tttatagaga tcgagcaaga tattgacggc aatcgcccgt gtgatcagcg
     8041 gatcattatt gaaactcgcc acccgttgcc gatcgtgggt caaatagcgt cttttcacat
     8101 aggaattaat aaaaaacaga ccacgcagac gatgccatag cgtcagcgca ggacgtgcca
     8161 gcggcacata caatttaacc ttaaaggccg gagaagccag caccagcccg cgaattgcag
     8221 gcgcataatc atgaacccat gtggcaacca gcactgcgcc gacgctttgc gcgatcacaa
     8281 ccacctcttc cagtccaacc tggctgtcgc tggcagcaaa acggacaaat tcatcgacat
     8341 cctgcactga acgcgcaaga gatgggctat aaccacgcgg ccccgaagtt tttccatgcc
     8401 cccgggcatc ccatgcataa aaagcagtat ctggcatcgc cagttcatca acgatatgtt
     8461 gtagacgacc agaatgttca tggccacgat gaaataagac gatgaccttt ttcgcccctg
     8521 gctgtaaagc gggccagtgc cgataaaaca acgctgtatt atcactggtg gtaaaaaaat
     8581 gttccccaga gatgcgtgaa ttttccattt tctctatttt ccttcagtaa cagcagtaac
     8641 gaataaaata aaaaaatacc ggagcggtga aaattaatga gtcaatcctg tcaaggattc
     8701 cgccatgtcc aggtaatagt ttcccgctat ctttaacacc aacatctcgt ttgatggctg
     8761 acatcactac atcgccgcaa aaaccactaa taccaattaa caaacccgct aatattgcct
     8821 gtaatgtatt tagcggtgtc agtaacggtc caatgatcat cgacgctatc atggtggtga
     8881 ttacgccgcc gaccagacct tccagcgttt ttcccgggct gactttgggg accacttttc
     8941 ttctgccgca ggatttcccc cataaatact gtgcaatatc gtttgacgcg gttaatgcca
     9001 aaagaaataa caccagtaag gccccgccct ggatattcat ggatggcaac attaataact
     9061 aggcggcatg actcaaggcg aaaacggtca ttatccagcc ccaaaaaatg gcgctcacgg
     9121 tatgcagaaa accggagggg tcacccacaa aaactcgcca ggcggctaat atcagaaacc
     9181 cagccaaagg tataaataac agaaacagct caaagcaatt aaaaccgata agcaaatagt
     9241 taagaggaat aacccaatat aaccaccgcg gaaaatgtac ggatataagc atacaatatt
     9301 ctttcaatgc cagaaaactt atcagcgcaa aaaacgccaa cgtcatccag cgtggacctg
     9361 aaatcaccag tgaaaaacaa atgacgatgc cccaccaggt acgaatacgt aatgtaacct
     9421 caccccct
//
LOCUS       DAJTYZ010000146         9422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000146 DAJTYZ010000000
VERSION     DAJTYZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.146"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            195..1061
                     /gene="folD"
                     /locus_tag="OO596_003480"
     CDS             195..1061
                     /gene="folD"
                     /locus_tag="OO596_003480"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6719251.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1063..1275
                     /gene="ybcJ"
                     /locus_tag="OO596_003481"
     CDS             1063..1275
                     /gene="ybcJ"
                     /locus_tag="OO596_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6719252.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1383..1904
                     /locus_tag="OO596_003482"
                     /pseudo
     CDS             1383..1904
                     /locus_tag="OO596_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1940..3325)
                     /gene="cysS"
                     /locus_tag="OO596_003483"
     CDS             complement(1940..3325)
                     /gene="cysS"
                     /locus_tag="OO596_003483"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6719253.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3499..3993
                     /gene="ppiB"
                     /locus_tag="OO596_003484"
     CDS             3499..3993
                     /gene="ppiB"
                     /locus_tag="OO596_003484"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6719254.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3996..4718
                     /gene="lpxH"
                     /locus_tag="OO596_003485"
     CDS             3996..4718
                     /gene="lpxH"
                     /locus_tag="OO596_003485"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6719255.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4836..5345
                     /gene="purE"
                     /locus_tag="OO596_003486"
     CDS             4836..5345
                     /gene="purE"
                     /locus_tag="OO596_003486"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6719256.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5342..6409
                     /gene="purK"
                     /locus_tag="OO596_003487"
     CDS             5342..6409
                     /gene="purK"
                     /locus_tag="OO596_003487"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6719257.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6546..7439)
                     /locus_tag="OO596_003488"
     CDS             complement(6546..7439)
                     /locus_tag="OO596_003488"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6719258.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7436..8089)
                     /locus_tag="OO596_003489"
     CDS             complement(7436..8089)
                     /locus_tag="OO596_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR6719259.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8100..9359)
                     /locus_tag="OO596_003490"
     CDS             complement(8100..9359)
                     /locus_tag="OO596_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR6719260.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDRAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2097 a         2672 c         2494 g         2159 t
ORIGIN      
        1 aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct ttcaatctca
       61 gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg aaacgaaacc
      121 tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac gtaacagatg
      181 gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg cagcaggtgc
      241 gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg gcaccaggac
      301 tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca agcaaacgca
      361 aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa accaccagcg
      421 aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc gatggcattc
      481 tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa cgtattcacc
      541 cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc cagcgcgcgc
      601 cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt tacaacattg
      661 ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt cgtccaatga
      721 gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc actaaaaatc
      781 tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag ccgggcttta
      841 ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc aaccgtctgg
      901 aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc gcctcataca
      961 ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt gaaaacacgc
     1021 tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac attttcttta
     1081 ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg gagcgaaagc
     1141 ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg cgcggttgaa
     1201 acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg tcacagcgta
     1261 caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc ttttcagact
     1321 tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc gaagcgacca
     1381 tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta gggttgagtt
     1441 cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg ctgcccgacg
     1501 ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt catcgcgggt
     1561 ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg attggacgcc
     1621 gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg ttgctatcgc
     1681 acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg tggccgtggt
     1741 cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt gcattgtctt
     1801 gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg gtgtggttgc
     1861 cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat gcgaaaactc
     1921 gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg ccatcttcca
     1981 gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc cagtctttcg
     2041 ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact tcgctgtcgt
     2101 ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg cctaatacag
     2161 cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct tctgctttca
     2221 gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg gtgttgaaat
     2281 cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca ggagcaacgg
     2341 ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc gcctgcttca
     2401 ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg aagtaacgca
     2461 cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta cccagcgatt
     2521 tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag ttaacatact
     2581 gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg aacatcaggt
     2641 cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc attgccgaac
     2701 attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc ggttcgccct
     2761 ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg accacgtcaa
     2821 cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca taagtcggat
     2881 cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga cctttggcga
     2941 tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc ggctccatat
     3001 ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc atgcgatcca
     3061 ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg tcgtcgatat
     3121 cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag cgcgcaacca
     3181 cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa acggtgattc
     3241 cacacacata catgccgact tccccggcgt gaataggctt aaattcctct ttttggcgtg
     3301 tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg ggtaattgag
     3361 ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat gatccgatcg
     3421 tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag caccaatcaa
     3481 acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat tgtcatcaaa
     3541 acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg ccgcgaaggt
     3601 ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca gggcggcggt
     3661 tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga agccaacaac
     3721 ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc gcactctgca
     3781 actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc tggcgaaagc
     3841 ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga cgtggtagac
     3901 aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc aaaagaagac
     3961 gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt attgcagatc
     4021 ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt ttagcggggg
     4081 aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg tggattggcg
     4141 acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg gtgtccgatt
     4201 ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc aaacgctttg
     4261 cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt tatggccgcc
     4321 gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat caggcttttc
     4381 gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg ttgtttgtgc
     4441 gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc agtaaatcgc
     4501 tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa catcaggtgc
     4561 aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc gccaaccagc
     4621 aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg gtgaaagtca
     4681 cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac tttgctgatt
     4741 tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt gccctctaaa
     4801 gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat aatccggcgc
     4861 gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc gccgccgaaa
     4921 tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac cgcacccccg
     4981 ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg attattgcgg
     5041 gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg gtgccggtgc
     5101 tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac tccatcgtac
     5161 aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc gcggcaaacg
     5221 cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac cagcgtctga
     5281 atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg cgaggtacgg
     5341 catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc gtcaggcagg
     5401 cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg cggcggtgcc
     5461 ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg cattaacccg
     5521 cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta ttgctgaccg
     5581 tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt ggcagttact
     5641 tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg cgattgttaa
     5701 gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca atgaaaccga
     5761 acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta acttctctgg
     5821 tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt atacgctgac
     5881 gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc aggccaacgc
     5941 acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc tgggctatgt
     6001 gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca acgaactggc
     6061 tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca gccagtttga
     6121 gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga ataatccgtc
     6181 ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat tgccgctggt
     6241 gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc atctgaattt
     6301 gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc cgctgctgcc
     6361 gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag caggtgctca
     6421 tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt acccattgta
     6481 ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc cggatgctct
     6541 catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc aattcgcgat
     6601 aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac cgccgttacc
     6661 ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg tgtggcatgg
     6721 cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc atcggtgagg
     6781 atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag atctttatca
     6841 atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc gccactgcaa
     6901 atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag aattttacgc
     6961 ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat ctgccagccg
     7021 taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat aaatttctct
     7081 ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac cgtcgtaaca
     7141 ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat catcccctgg
     7201 ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc cagattctgt
     7261 aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg ataagaacgg
     7321 gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt ttccgccgtc
     7381 agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag tgttttcatg
     7441 gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct gcccctgacg
     7501 tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg caactcagag
     7561 catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa cgtaaataac
     7621 tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa aactggcgta
     7681 actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc ccggtttcct
     7741 ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc gtaatacgca
     7801 gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc ccttgagcca
     7861 caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat tgcgcgcggc
     7921 gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt aacagttcgc
     7981 cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc caggactggc
     8041 gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt gcgcgttcct
     8101 caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca cgccagcggt
     8161 gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc gataccggta
     8221 ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc caggcacgcg
     8281 ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta aatttccgcc
     8341 atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa gcgcgttacg
     8401 ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat cgctctatcg
     8461 cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc ttgcggagtg
     8521 tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc gaacatattg
     8581 ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc agcatccatc
     8641 gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac gctgaggaaa
     8701 tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg tggggccagc
     8761 gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc gcccatcgta
     8821 atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc cagcgccgcg
     8881 cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac atcttcgccg
     8941 taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta cgcccggttg
     9001 ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat accgcccatt
     9061 ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg ctccagtatt
     9121 gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc cacgcacgcc
     9181 cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg aagcagtgtt
     9241 ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag ccagtgtgga
     9301 cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga tgtaaacatg
     9361 ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca acttattgat
     9421 ag
//
LOCUS       DAJTYZ010000147         9217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000147 DAJTYZ010000000
VERSION     DAJTYZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.147"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..732
                     /locus_tag="OO596_003491"
     CDS             73..732
                     /locus_tag="OO596_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6719261.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="OO596_003492"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="OO596_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6719262.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="OO596_003493"
     CDS             complement(1860..2570)
                     /locus_tag="OO596_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6719263.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="OO596_003494"
     CDS             3004..3294
                     /locus_tag="OO596_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR6719264.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="OO596_003495"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="OO596_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6719265.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="OO596_003496"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="OO596_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6719266.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="OO596_003497"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="OO596_003497"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6719267.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="OO596_003498"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="OO596_003498"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6719268.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="OO596_003499"
     CDS             complement(7538..7837)
                     /locus_tag="OO596_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6719269.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="OO596_003500"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="OO596_003500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6719270.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9217)
                     /locus_tag="OO596_003501"
     CDS             complement(9049..>9217)
                     /locus_tag="OO596_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719271.1"
                     /translation="MVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPK
                     ETQVTQEEYSE"
BASE COUNT         2287 a         2331 c         2268 g         2331 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc taccatc
//
LOCUS       DAJTYZ010000148         9213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000148 DAJTYZ010000000
VERSION     DAJTYZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.148"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..722)
                     /gene="glsB"
                     /locus_tag="OO596_003502"
     CDS             complement(<1..722)
                     /gene="glsB"
                     /locus_tag="OO596_003502"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6719272.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMAT"
     gene            complement(786..2174)
                     /gene="sad"
                     /locus_tag="OO596_003503"
     CDS             complement(786..2174)
                     /gene="sad"
                     /locus_tag="OO596_003503"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCR6719273.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2275..3156
                     /locus_tag="OO596_003504"
     CDS             2275..3156
                     /locus_tag="OO596_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6719274.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3233..4348
                     /gene="yneK"
                     /locus_tag="OO596_003505"
     CDS             3233..4348
                     /gene="yneK"
                     /locus_tag="OO596_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6719275.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4498..5688
                     /gene="ydeA"
                     /locus_tag="OO596_003506"
     CDS             4498..5688
                     /gene="ydeA"
                     /locus_tag="OO596_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6719276.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(5713..6378)
                     /gene="marC"
                     /locus_tag="OO596_003507"
     CDS             complement(5713..6378)
                     /gene="marC"
                     /locus_tag="OO596_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6719277.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6590..7024
                     /gene="marR"
                     /locus_tag="OO596_003508"
     CDS             6590..7024
                     /gene="marR"
                     /locus_tag="OO596_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6719278.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7044..7427
                     /gene="marA"
                     /locus_tag="OO596_003509"
     CDS             7044..7427
                     /gene="marA"
                     /locus_tag="OO596_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6719279.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7459..7677
                     /gene="marB"
                     /locus_tag="OO596_003510"
     CDS             7459..7677
                     /gene="marB"
                     /locus_tag="OO596_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6719280.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKPDALGVPYYNQHAM"
     gene            complement(7734..9173)
                     /locus_tag="OO596_003511"
     CDS             complement(7734..9173)
                     /locus_tag="OO596_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6719281.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2372 a         2350 c         2076 g         2415 t
ORIGIN      
        1 ctggtcgcca tcagcgcgtt aatttgccgc gcctgcattg gcgtcactac aggttcatca
       61 atatgaatag ctttcccctg attagccaga aagacaaacg tccgagccag ctctacacag
      121 ctcattttca gagcgcagta atgaaagtag ttttgcagaa cggttgtcac gtcatgatgg
      181 aaattgccaa acgacttcat cagccaggcg atagccgcat ttcgcgcgga atgttcaaat
      241 tcggaacgcg ctaccaccgt atcgtatgaa atatcagaca caccgcttaa gccgcgcacg
      301 acttccagca tacgttgccg tggtgcgctt aatcgccctt gcaacatatc gcagaccacc
      361 agcgcaccag cattaataaa cggattacgc ggaataccct gttccatttc cagttgcact
      421 aaagaattga acggtgatcc agacggatct ttgccgacgc gttgccagat ttcctcttcg
      481 gagtaatgac gcatggcgac aacgagactc agcactttgg aaatagactg aatggaaaaa
      541 cgttcttgcg cgtctccggc ctgaaaaagc tgtccgtcaa cggtacagat agcaatgccc
      601 aatcgggaac cgtctactgt agccagcgcc ggaatataat ccgcgacttt accctgacca
      661 atgagcggcc gcacttgccg caagatgttt tctaaaattg cattatccat ggcgactgcc
      721 actttctact cctggaccgc aggtctgaaa agacctgcga gtatatcaga gctgaatatg
      781 tcgcgtcaga tccggtcttt ccacaccgtc tggatattac agaattcgtg taagccgaaa
      841 tgggaaagct cacgaccaaa gccactcttt ttcacgccac caaaggccac tcgcgcgtcg
      901 ctggcacaat aaccattgat aaacacccca ccgcattcca gacgtgccgc catctgtctg
      961 gcctgtgttt cgtcggtggt aaaaatggtc gctgaaaggc cgaactcact atcattagcc
     1021 agttccagcg catgttctgc atctttcgca acggtgattg ccgcaacggg gccaaacatt
     1081 tcttcccgaa acgcggtcat ttctggggta acattcgcca gaaccgttgg cggatagtag
     1141 ttacctgccc cagccatctt ttccccgccc agtaacaaac gcgcaccctg cgccagggtt
     1201 ttctctacct gatgatgcag ctcatcacgt aaatcaaaac gagccattgg tccgagagtg
     1261 ttctcttcgt cacggggatc gcccattttc aaggctgccg cagctgccac aaaacgttcg
     1321 gtaaatgcag aagcaattcc ctcttcgata ataaagcgtt ttgccgctgc acatacctgt
     1381 ccggtattct gataacgtcc ggctaccgcc gctttaaccg ccagttccag atcggcatcg
     1441 ttaagcacaa taaacggatc cgaaccgccc agttccagta cgcatttttt cagtgccgct
     1501 ccagcctgtg cgccaatagc cgctccagca cgaacacttc cggtcaccgt gacagcagca
     1561 atgcgcgagt ctttaatcat ctgactgaca ccgtcgttgt cggcattcag ccagccatat
     1621 acgccttgcg ggattcccgc atctttaaac acctgggcaa tgagctgagc acatcccatc
     1681 acattcggcg catgtttaag taagtagccg ttacctgcaa gaatgatggg aacagcgcca
     1741 cgcatcacct gccataacgg aaaattccac ggcataatcg ccagaatcgt ccccaacggt
     1801 cgatactcaa taaccgcctg ctgattttcc accagtgtag gttccgcctt cagcattgcc
     1861 ggaccatgtt ctgcatacca gtcacacaaa ttcgccgatt tcgccacttc agcgcgcgcc
     1921 tggttgattg gtttgcccat ttcgcgggtg atcatttgcg ccatttcttc gctacgggcg
     1981 cgcagagcct taccgatatc acgcattttt tcagcacgat aatctatatt tgtctcgcgc
     2041 cagtcgcgaa agcctgctgc cgccagctca agtgcgtttt cgatatcgtt agcgccagcc
     2101 cacggcagca cagaaagttg ttcacccgtg gcaggattta ttgaaattgc atgagttgcc
     2161 ggagtaatgg tcatcggggt atctccttta tgagtcatgg tatgaagata cgcagattta
     2221 ctcttgcttt aaaatgaata atattaagcc acttattcac gaatcgagaa tgttatggat
     2281 ctgacccaac tggagatgtt caacgcggtt gccgaagctg gcagcattac ccaggctgca
     2341 gcaaaagtgc atcgcgtacc gtcgaatctc actacccgtc tacgccagct ggaaacagaa
     2401 ctgggggtag atctgtttat tcgcgagaat cagcgtttac gtctctctcc tgccgggcat
     2461 aactttttac gctatagcca acaaattctc gcgttagtgg atgaagcgcg gagcgttgtc
     2521 gctggcgatg agccgcaagg tttattttct cttggttcgc tggaaagcac cgcagcagtg
     2581 cgcattccgg ccacgctggc ggaattcaac catcgttatc ccaaaattca gttttcactt
     2641 tccaccggcc cttccggcac tatgctggaa ggagtgctgg agggaaaact gaatgcggca
     2701 tttattgatg ggcccattaa ccatactgcc attgacggga taccggtata ccgcgaggag
     2761 ctgatgatcg tcacgccaca aggtcatgcg ccagtaatcc gcgccagtca ggttaacggc
     2821 agtaacattt atgccttccg caccaattgt tcgtatcgtc gccacttcga gagctggttt
     2881 catgctgacg gtgccgctcc gggaactatt cacgagatgg agtcgtatca cgggatgttg
     2941 gcctgtgtgg tcgcaggagc aggcattgcg cttattccac gctctatgct ggaaagtatg
     3001 ccggggcatc accaggttga agcgtggccg ttagctgagc aatggcgttg gttaacaacc
     3061 tggctggtct ggcgtcgcgg tgcgaaaacg cgtccgcttg aggcatttat tcaactactg
     3121 gatgtgtctg actcggcaaa acagagttac caatgaacta ttttgataat tcttgcggct
     3181 aatatgctct atatagtgat gttccgatga cttatgacta tatggggcaa atatggttac
     3241 gccagtaagc atcagcaatt acatatctct tcctgatgat tttcccgttc gtaatattgc
     3301 accacaggta aaagaagttt taaaggactt tattgatgcg ctaagtacaa taatatgtga
     3361 tgaagagtgg cgcacgtctt taaacatcaa ctcagccaca aaaaagatat ttaataacct
     3421 tgataactta tcatatattc agaggacttc tttccggggt aacgacacgc tatacaatga
     3481 aaaggttcag tttaaactca cttatcccgt caagaatgga agacacaaag aaaatattga
     3541 atttcaggta gtaataaatt taagccctat ttatttagat aattttcgcc atgatggaga
     3601 aattaatatt ttttgcgctc ccaacccaaa gcctgtcact atggggcgcg tatttcagac
     3661 cggcgtcgag cgtgtgctct ttctgtttct gaatgatttt attgaacaat ttccaatgtt
     3721 caaccctggc gttccgatca aaatagcgca tacaccacat attgaacccc tgcctccgga
     3781 tcaccatacc gctgcagatt acttacgcca gtttgatttg cttgtcctga attttatctc
     3841 tcgcggtaat tttgtcatac tcccccgatt atggaataac tctgaggttc acagatggtt
     3901 cgtcaataaa gatcctaact tgatcaccgc cattctcgac ataacggaca gcgaattaaa
     3961 agaggattta ttgcaaagcc tgatggattc attaggttct aacaaacatg tactacccga
     4021 agtctgcatc tgctttttat tccttttagc agaacaagaa tctcctcatt ttcaggactt
     4081 gtttttattt ttcgccaata tgttattgca ctatcaccaa tttatgaatc ccaatgaaag
     4141 tgatttgaat gacgtgttga tgccagcatc attaagtgat gataaaatta tcaagcatat
     4201 ggcgcgcagg accctcgaac tgtttgtaaa aaatgaaaca ccgccaaaag ttactcacga
     4261 agacctggtg aaaaacaggc ctcgctcccc tgtcagacca cctatacccg caacctccaa
     4321 aacgccagac ctccctgaac gtcattaaac cgtgatgtta ccgactctct gacgcggaac
     4381 agaatcagcg tcagagaaac ggaaaacgcg atccagatca caaatacatt gtattcacat
     4441 cattaaccgt tttaagatca tttcatcact ttttcgcaac tcacccgata atctgttatg
     4501 acaacaaaca ctgtttcccg caaagtggcg tggctacggg tcgttacgct ggcagtcgcc
     4561 gccttcatct tcaacaccac cgaatttgtc cctgttggcc tgctctctga cattgcgcaa
     4621 agttttcaca tgcaaaccgc tcaggtcggc atcatgttga ccatttacgc atgggtagta
     4681 gcgctaatgt cattgccttt tatgttaatg accagtcagg ttgaacggcg caaattactg
     4741 atctgcctgt ttgtggtgtt tattgccagc cacgtactgt cgtttttatc gtggagcttt
     4801 accgttctgg tgatcagtcg cattggtgtg gcttttgcac atgcgatttt ctggtcgatt
     4861 acggcgtctc tggcgatccg tatggctccg gccgggaagc gggcacaggc cttgagttta
     4921 attgccaccg gtacagcact ggcgatggtc ttaggtttac ctctcgggcg cattgtgggg
     4981 cagtatttcg gttggcgaat gaccttcttc gcgattggta ttggggcgct tatcaccctt
     5041 ttgtgcctga ttaagttact tcccttactg cccagtgagc attccggttc attgaaaagc
     5101 ctcccgctat tgttccgccg cccggcattg atgagcattt atttgttaac tgtggtggtt
     5161 gtcaccgccc attacacggc atacagctat atcgagcctt ttgtgcaaaa cattgcggga
     5221 ttcagcgcca actttgccac ggcattactg ttattactcg gtggtgcggg cattattggc
     5281 agcgtgattt tcggtaaact gggtaatcag tatgcgtctg cgttggtgag tacggcgatt
     5341 gcgctgttgc tggtgtgtct ggcactgctg ctacctgcgg cgaacagtga aatacacctc
     5401 ggggtgctga gtattttctg ggggatcgcg atgatgatca tcgggcttgg tatgcaggtt
     5461 aaagtgctgg cgctggcacc agatgctacc gacgtcgcga tggcgctatt ctccggcata
     5521 tttaatattg gaatcggggc gggtgcgttg gtaggtaatc aggtgagttt gcactggtca
     5581 atgtcgatga ttggttatgt gggcgcggtg cctgcttttg ccgcgttaat atggtcaatc
     5641 attatatttc gccgctggcc agtgacactc gaagaacaga cgcaatagtt gaaaggccca
     5701 ttcgggcctt ttttaatggt acgttttaat gatttccagg atgccgttaa taataaactg
     5761 cacacccata cataccagca ggaatcccat cagacgggag atcgcttcaa tgccaccctt
     5821 gcccaccagc cgcataattg cgccggagct gcgtaggctt ccccacaaaa taaccgccac
     5881 caggaaaaag atcagcggcg gcgcaaccat cagtacccaa tcagcgaagg ttgaactctg
     5941 acgcactgtg gacgccgagc taataatcat cgctatggtt cccggaccag cggtacttgg
     6001 cattgctagc ggcacaaagg caatattggc actgggttca tcttccagct cttccgactt
     6061 gcttttcgcc tccggtgaat caatcgcttt ctgttgcgga aagagcatcc gaaaaccgat
     6121 aaacgcgacg attaagccgc ctgcaattcg cagaccggga atcgaaatgc caaatgtatc
     6181 catcaccagt tgcccggcgt aatacgccac catcatgatg gcaaatacgt acaccgaggc
     6241 catcaacgac tgacgattac gttcggcact gttcatgttg cctgccaggc caagaaatag
     6301 cgcgacagtt gttaatgggt tagctaacgg cagcaacacc accagcccca ggccaattgc
     6361 tttaaacaaa tctaacattg gtggttgtta tcctgtgtat ctgggttatc agcgaaaagt
     6421 ataaggggta aacaaggata aagtgtcact ctttagctaa ccttgcatcg cattgaccaa
     6481 aacttgaacc gatttagcaa aacgtggcat cggtcaattc attcatttga cttatacttg
     6541 cctgggcaat attatcctct gcaactaatt acttgccagg gcaactaatg tgaaaagtac
     6601 cagcgatctg ttcaatgaaa ttattccatt gggtcgctta atccatatgg ttaatcagaa
     6661 gaaagatcgt ctgcttaacg agtatctgtc tccgctggat attaccgcgg cacagtttaa
     6721 ggtgctctgc tctatccgct gcgcggcgtg tattactccg gttgaactga aaaaagtgtt
     6781 gtcggtcgac ctgggagcac tgacccgtat gctggatcgc ctggtctgta aaggctgggt
     6841 tgaaaggttg ccgaacccga atgataagcg cggcgtactg gtaaaactta ccaccagcgg
     6901 cgcggcaata tgcgaacaat gccatcaatt agttggccag gacctgcatc aagaattaac
     6961 aaaaaacctg acggcggacg aagttgcaac acttgagcat ttgcttaaga aagtcctgcc
     7021 gtaaacaaaa aagaggtatg acgatgtcca gacgcaatac tgacgctatt accattcata
     7081 gcattttgga ctggatcgag gacaacctgg aatcgccact gtcactggag aaagtgtcag
     7141 agcgttcggg ttactccaaa tggcacctgc aacggatgtt taaaaaagaa accggtcatt
     7201 cattaggcca atacatccgc agccgtaaga tgacggaaat cgcgcaaaag ctgaaggaaa
     7261 gtaacgagcc gatactctat ctggcagaac gatatggctt cgagtcgcaa caaactctga
     7321 cccgaacctt caaaaattac tttgatgttc cgccgcataa ataccggatg accaatatgc
     7381 agggcgaatc gcgcttttta catccattaa atcattacaa tagctagttg ataacgtgac
     7441 aacgtcactg aggcaatcat gaaaccactt tcatccgcaa tagcagctgc gcttattctc
     7501 ttttccgcgc agggcgttgc ggaacaaacc acgcagccag ttgttacttc ttgtgccaat
     7561 gtcgtggttg ttcccccatc gcaggaacaa ccaccgtttg atttaaatca catgggtact
     7621 ggcagtgata agccggatgc gctcggcgtg ccctattata atcaacacgc tatgtagtta
     7681 ttgtcaggct ctgatcacag agcctgacat ctccgtaata tcttcctcaa gtctcaaata
     7741 ttttcgccat tgttggcgat gagatcccga taccagtaaa acgacgcttt acgtatccgt
     7801 tccagcgttc ccttcccttc gttgtcttta tcgacataaa tcattccgta gcgctttttc
     7861 atttcccctg ttccggcaga aaccaggtca atgcagcccc acggggtgta gccaattaag
     7921 tcaacaccat cttcaactac ggcttttttc atttcccgaa tatgggaagc aaagtaatca
     7981 atgcgatagt gatcgttcac cgtgccgtca gcttgtctct ggtcaaccgc accaaatcca
     8041 ttttcgacaa taaacagcgg caactggaaa tgatcccaga accagttgag tgaataacgt
     8101 agccctgccg gatcaatttg ccatccccat tcggatgtat cgatataagg gttgctgacc
     8161 aaatctcgtg gttcaacata atccagttgc gggttatcgg gagaaaattg tgtcgtaaaa
     8221 gacatgtagt agctaaaacc gataaagtcg acacatccac tggcaagaat cgcgttatct
     8281 tctggtgtga tatcgaggtt gaatcctttc ctggcaaagt aattcagcat atgttgcgga
     8341 taatatccac gagcatgaac atcagtaaac cagtaacgac gatgcatcgc tttcgtggcc
     8401 atcatcatat cgttgggtgc acacgtcaga ggatagatgg ggcacatggc gatcatacag
     8461 ccaatgttaa attcaggatt gatcgatttt ccagtctgta ccgccagggc actggcaact
     8521 aactcgtaat gtaccgcctg gtacattatt tgttcgcgct cattgatatc ttcctctggc
     8581 gaatacaaga taccggaatt agtaaatgga cacaggcttt cgctgaaatt cacctgatta
     8641 ttgatttcgt taaacgtcat ccagtacttt actttttctt tatagcgcgt gaagaccgtt
     8701 gatgcgaagc ggataaaaaa gtcgatcagc ttacggtttc gccagccacc atattttgtc
     8761 accagatgat aaggcatctc aaaatgcgaa agcgtcacca caggttccat tccttgcttc
     8821 aggcattcat cgaacagatc atcataaaat tgtaaaccct cttcattcgg ctcctgttcg
     8881 tcaccttgcg gaaagattcg tgtccaggca atggaagttc gaaagcattt gaatcccatc
     8941 tcggcaaata actgaatatc tgttttatag cgatgataaa aatcaattgc ttcatgattg
     9001 ggataattaa gcccgtcgat aacgccttct gtcacttcac gcggcacccc gtgagcgcca
     9061 gcagtcatca catcagcgat actgatgcct tttcctcctt cattccagcc accttccaac
     9121 tgatgcgcgg ctaccgcgcc accccataaa aaaccttttt taaatcctga catacacaac
     9181 tccttaacta aaatagatta ccaccagact tcc
//
LOCUS       DAJTYZ010000149         9185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000149 DAJTYZ010000000
VERSION     DAJTYZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.149"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="OO596_003512"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="OO596_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="OO596_003513"
     CDS             complement(1751..2509)
                     /locus_tag="OO596_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6719282.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="OO596_003514"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="OO596_003514"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6719283.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="OO596_003515"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="OO596_003515"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6719284.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="OO596_003516"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="OO596_003516"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6719285.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="OO596_003517"
     CDS             complement(5474..6079)
                     /locus_tag="OO596_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719286.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="OO596_003518"
     CDS             complement(6082..6849)
                     /locus_tag="OO596_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719287.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="OO596_003519"
     CDS             complement(6852..7562)
                     /locus_tag="OO596_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719288.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="OO596_003520"
     CDS             complement(7578..9083)
                     /locus_tag="OO596_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6719289.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2359 c         2260 g         2194 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctt
//
LOCUS       DAJTYZ010000150         8874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000150 DAJTYZ010000000
VERSION     DAJTYZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8874
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.150"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(350..1603)
                     /locus_tag="OO596_003521"
     CDS             complement(350..1603)
                     /locus_tag="OO596_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6719290.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="OO596_003522"
     CDS             1710..2603
                     /locus_tag="OO596_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6719291.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="OO596_003523"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="OO596_003523"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6719292.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="OO596_003524"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="OO596_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6719293.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OO596_003525"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OO596_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6719294.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALSASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OO596_003526"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OO596_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6719295.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="OO596_003527"
     CDS             complement(6839..7693)
                     /locus_tag="OO596_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6719296.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OO596_003528"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OO596_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6719297.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>8874)
                     /locus_tag="OO596_003529"
     CDS             complement(8323..>8874)
                     /locus_tag="OO596_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCR6719298.1"
                     /translation="FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2336 a         2257 c         2308 g         1973 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcgga aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaa
//
LOCUS       DAJTYZ010000151         8861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000151 DAJTYZ010000000
VERSION     DAJTYZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.151"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            375..1250
                     /locus_tag="OO596_003530"
     CDS             375..1250
                     /locus_tag="OO596_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR6719299.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1258..2034
                     /locus_tag="OO596_003531"
     CDS             1258..2034
                     /locus_tag="OO596_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR6719300.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2203..4464
                     /locus_tag="OO596_003532"
     CDS             2203..4464
                     /locus_tag="OO596_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6719301.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4533..5708
                     /gene="senB"
                     /locus_tag="OO596_003533"
     CDS             4533..5708
                     /gene="senB"
                     /locus_tag="OO596_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR6719302.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6234..6695)
                     /locus_tag="OO596_003534"
                     /pseudo
     CDS             complement(6234..6695)
                     /locus_tag="OO596_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(6733..8271)
                     /locus_tag="OO596_003535"
     CDS             complement(6733..8271)
                     /locus_tag="OO596_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6719303.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPMEEALHGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
     gene            complement(8321..8668)
                     /gene="tnpB"
                     /locus_tag="OO596_003536"
     CDS             complement(8321..8668)
                     /gene="tnpB"
                     /locus_tag="OO596_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6719304.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT         2315 a         2197 c         2151 g         2198 t
ORIGIN      
        1 ttaacgtacg ttttcgttcc attggccctc accccccgat aacgacccag gtagcgcgcc
       61 gtattccccc cgccggccgt tttcttcgac atgtacatca ggcgcggaac tcatcggggc
      121 gcgggaatga aatcccattc agactccgga tagattcaag caagcccact tatcgtcaaa
      181 atcggtgttg caaaaacggg agtgaccata gatccgtttt ccaagcggac cccataatat
      241 cggctggtaa acatcactcg ctagtgcatt ctttttgttg gtaatgataa tcatttacat
      301 tcattctttg atgttttatc ataattaatg ttttgtcaat aaatatatgg aaacatattg
      361 tttcagatgg gaatatgaga aagttcatcc ttatttctat gataacgtta gctagtgtta
      421 gtctgggagc ctgcagacag aatgtcacca taaagcagga tgcgccaggg cagaaagctt
      481 tcctgactga cgggcagatt tacgatttgc acagtggcaa aattatttct agcagcgagc
      541 ttttggcgga tcttgctacc gctcgacatc ttattattgg tgaaaaacac gataatgccg
      601 agcatcatca gattgagctt tggcttattc aaaatttgct catccagcga ccacagggga
      661 gtgtgttgct tgagatgttg acgtcagagc aacagccccg agttaaccaa gttaaatgtt
      721 ggctgaaaga taatcctgtg gttcgagata gccgtgttca ggaactactt aactggcaaa
      781 aagggtggtc ctgggagatg tatggcgaca tcgtcatgca gctattacgt gggccttacc
      841 cgctgctcaa tgccaacatt ggccgggagc agatgttggc actgtacaaa aaaaacgaat
      901 ttcctaaagg aaaaaaatcc actgcacctg tggtacaaga agctctgcgt gaaaccatca
      961 tatctatgca tgaaggcaac cttgaaagcc agcaactgac gtcaatgctc tctattcagc
     1021 agcagcggga tcgctacatg gcccgccagc ttctgagtgc cccggtaccg tcgttgttga
     1081 ttgcaggtgg ttatcacgcg tcaaaaagca tgggcgtacc gttacacatg gaagatcttg
     1141 ccaccggcac ccatccagtg gtgttgatgc ttgcagaaaa aggaatgaac atcacagtag
     1201 atcatgctga ctacgtgtgg tttgtagcgc cagatacaac aaaaaggtaa accatggatg
     1261 atgaatattc tccacttctc acagtcggta aaatggagca gctggtttat ctgttctcta
     1321 ctgcttcatg gactgatttt ccttgcgttt atctggcgtt tttccgaagt tcagcccgct
     1381 atgtctcctg caccggcgat catgctgcaa tgggcagaaa ttatagaggc tccttcttct
     1441 ccactttcgc ttccggtggg aatcgctcaa caggagtccg cagtgacaga agaaaagcaa
     1501 caaactgagg acagacaaca acgtcctgtc actgaagaca gtgatgcaac cattgaaata
     1561 actaggaaga aaaagtcatc cgatggagaa aagaaaaaga cacgtccacc tcgtaagata
     1621 aaggctcaaa ccagcgacag caaccccaca gccgtttcca gtaatgctgc acctcaggct
     1681 ctggtcgaat cgtcccgtat tgctgcgcct tttaacagtg actcaacaaa gcgagataat
     1741 tctgaggcat cttgggaaag cagagtaaaa ggccacctaa accgttataa acgttaccct
     1801 ggcgatgcca gaaaacgtgc acgaaccggc acagccgtcg tgacttttac cgtaaacacg
     1861 gaaggcacta ttgtcagtag tttcttggaa atctcttcag gaaccctttc tcttgaccgc
     1921 gaagctattg ctgttttaga acgcgctcaa ccacttccca aaccaccgcc tgaaatattg
     1981 gagggaggat tatttaaagt aaaaatgccg attaccttta agttgaagga gtaaagggat
     2041 aattggccgt tcaagctcat ttaactgaaa tttaattttc atttttatgc tgaacatttt
     2101 atcaaacttt tccactgtcg aaaaaatcaa aaagacagta gaacataatt aataaacaaa
     2161 tagttatact tatctttatt aatattatac ccaaggaaat ttatgaacgt tataaaactg
     2221 gctatcggct caggcatatt attgctcagc tgcggtgctt actcacaatc catcagtgaa
     2281 aaaactaatt ccgacaaaaa aggagcggca gaattcagtc cgctcagcgt ttctgtcggg
     2341 aagacgacca gtgagcagga agctctcgag aaaacaggcg cgaccagttc ccggacaacg
     2401 gacaaaaacc tgcaatcact tgacgcaaca gtgcgtagta tgcctggtac ttatactcaa
     2461 atagatcctg gtcagggagc aatcagtgtg aatattcgag gcatgagcgg atttggtcgt
     2521 gtaaacacta tggtcgatgg tattacccag agtttttacg gaacctctac ctccggaaca
     2581 acgacgcatg gttcaactaa caatatggct ggcgtactta tagatcctaa cttactggta
     2641 gcagttgatg ttacacgcgg tgacagcagt ggctctgaag ggatcaacgc ccttgccggt
     2701 agtgcaaata tgcgtactat tggcgttgac gatgtaatat ttaacggtaa tacatatggc
     2761 cttcgttcac gtttctctgt cggtagtaat gggctgggac gcagcggaat gatcgccctt
     2821 ggtggaaaaa gcgacgcttt tacggatacg ggaagcattg gcgttatggc tgctgtgagc
     2881 ggcagttctg tgtactctaa tttctcaaat ggttctggaa ttaacagcaa agagtttggt
     2941 tatgataaat atatgaagca gaaccccaaa tcccaactgt ataaaatgga tatcagacca
     3001 gacgaattta acagcttcga actttccgct cgaacctatg aaaataaatt tacacgtcgt
     3061 gatataacca gtgacgacta ttacattaaa tatcattaca cccctttttc tgaattaatt
     3121 gactttaacg taacggccag taccagtcgc ggtaatcaaa agtatcgtga tggctcgctg
     3181 tatactttct acaaaacctc agcgcaaaat cgttctgacg cgctggatat caacaatacc
     3241 agccggttca ctgtcgcgga caatgacctg gagtttatgc tgggcagcaa actgatgcgt
     3301 acccgctatg accggaccat tcactcagcg gcgggcgacc cgaaagcgaa tcaggaatcg
     3361 atcgagaaca atccgttcgc accctccggc cagcaggata tttcagcgct gtataccggg
     3421 ctgaaggtta cgcgcggcat ctgggaggca gatttcaatc tcaactacac acgtaacagg
     3481 atcacagggt acaagcccgc ctgcgattca cgcgttatct gcgtgccaca gggtagctac
     3541 gatattgacg ataaagaggg tggcttcaac ccttcagttc agctttctgc tcaggtaaca
     3601 ccatggcttc agccgttcat tggctacagc aaatccatgc gcgccccgaa catccaggag
     3661 atgttcttct ctaattcagg aggcgcatcc atgaacccat tcctgaagcc tgaacgtgca
     3721 gaaacctggc aggcgggttt taacattgat accagagatt tactggtcga acaggatgcc
     3781 ctgcgcttta aggctctggc gtaccgcagc aggatccaga actacatcta cagcgagtct
     3841 tatctggttt gttctggagg tcgtaaatgc agtatgcctg aggtgattgg caatgactgg
     3901 gagggcatta gcgatgaata cagcgacaat atgtacatct acgttaactc ggcaagcgac
     3961 gttatcgcaa agggcttcga actggagatg gattatgatg caggttttgc ttttggccga
     4021 ctctctttca gccagcagca aacagaccag ccaacctcca tcgccagcac ctactttggc
     4081 gcaggggata tgaccgaact gcccagaaaa tacatgacgc tggatactgg tgttcgcttc
     4141 ttcgataacg cgttgaccct gggcactatc ataaaataca caggcaaggc tcgtcgcctg
     4201 tcgcctgatt ttgagcagga cgaacatacc ggcgcaataa tcaaacagga tttgccgcag
     4261 atcccaacga ttatcgatct ctatggtact tacgagtaca accgcaacct aacactgaaa
     4321 ctttcggtac aaaacctgat gaacagagat tattcggagg cgctgaataa gctcaacatg
     4381 atgccaggtc ttggtgacga gacccaccca gccaattccg cgcgtggcag aacatggata
     4441 tttggcgggg acattcgttt ctgatgacaa tttccgctag gttcagacaa tacgttttct
     4501 ctcttatgtc aattttattg caggaacgaa aaatgaatat tttcacttta tccaaagcac
     4561 cgctatacct gttaatttca ctatttttac ccacgatggc catggctatc gatccacctg
     4621 aacgcgaact ttcgcgattt gccctgaaaa cgaattacct tcagtcccct gatgaaggcg
     4681 tctatgaact ggcgtttgat aatgccagta aaaaggtgtt tgcagcagtc accgatcgtg
     4741 taaatcgtga agccaataaa ggctatctgt attcgtttaa ttcagattcg ctgaaagtcg
     4801 aaaataaata cacgatgcca taccgggcat tttcgctggc gataaatcag gataaacatc
     4861 agctctatat cggacacacc cagtcagcgt ccctgcgtat cagtatgttt gacaccccaa
     4921 ccggcaaact ggtaagaacc agcgacaggt taagttttaa agcggcaaac gctgcagatt
     4981 cgcgttttga gcattttcgc catatggttt acagccagga ttccgatacc ctgtttgtga
     5041 gttatagcaa tatgctgaaa acggccgagg gcatgaagcc tctgcataag ctgttaatgc
     5101 tcgacgggac gacgcttgcc ttaaaaggcg aggttaagga tgcttacaaa ggtacagcgt
     5161 atggtctgac gatggatgaa aaaacacaga aaatctacgt tggcggaaga gattacatca
     5221 acgaaattga tgcgaaaaat cagacgctgc tgcgtaccat cccgttgaaa gatccgagac
     5281 cacaaatcac aagtgtgcag aatctggcgg tggactccgc ttctgaccgt gcctttgtgg
     5341 tggtattcga ccatgacgat cgttccggta caaaagatgg actctatatt tttgacttac
     5401 gcgacggtaa acagcttggc tatgtgcaca caggagccgg agctaacgcg gtgaaataca
     5461 atccgaaata taacgaactg tatgtcacca acttcactag cggcaccatc agcgtagtgg
     5521 atgccaccaa atacagcatc acccgtgaat ttaacatgcc ggtctaccca aaccagatgg
     5581 tgttgtcgga cgatatggat accctttaca ttggcatcaa agaaggcttt aaccgcgatt
     5641 gggatcctga tgtgtttgtg gaaggagcta aagaacgtat tctgagcatt gatttgaaaa
     5701 agtcgtgatc ggggggaaaa aacatgggat aaaaactccg ttattccact aaactggccg
     5761 gactaggaaa agcaaacatc tctgtcctgc cggtacgcct gttacgccgc tccatgaggc
     5821 tgaggaatct ccagaaaacg agacaggcac agcattgtac gatcaactga tttgtgccaa
     5881 taattaaatg aaatcacagc tatgcctgtt catggggttc taggccggaa ccccagattt
     5941 ttccgattgt ttgttttcta actgattatt cagtagtttt agagggctga cgctcagtgg
     6001 aacgaaaact cacgttaaga ggttttgact atgataaaag tcaaagtagg ggtgagtttg
     6061 gatatggaaa ttattgtact ataaggagag catgaaaacc cctggggcag tcactgaatg
     6121 agggaccagc agcggtaaaa taaccagcat aatttctgca tggtcgattt ttataccgtg
     6181 acaggctgtt ttttatactt ggggtcagtt tggatatcga aaattattgt acgttaaggt
     6241 tgttttttga ccttgtcgat cttaactgat ctcaaacgac gaaacttacc tgaagtcggt
     6301 atccggctgt ttttccagat ataatcgcct gtcagattga tatgttccca acctaatggt
     6361 gataaatgcg ataatagttg agcatttatt ttcatgccct tacgcttcag agcatcaacc
     6421 gctctttcca tgtaaactgt gttccataag gaaatagccg ccgtcaataa agtcaatcca
     6481 ctggcgcgat aactttgatt ttccggtttc cgatctctaa tctcccccaa tcgatgcatg
     6541 aatacagcac gggccagagc attacgggct tctcctttgt tcagcccggc ctggacacgt
     6601 cgccgcaatg ccggatcccg aaaccagtcc agcataaaca gtgttcgttc tatacggcca
     6661 atctctctca gagctttagc caacccattt tgtttgtaag cgtgcagcaa gaaccgtatt
     6721 gacgggaatg tgttattcag tcggcagtgc tacgcgccag gggagcagtt cgccgacccg
     6781 gtttatcggc cagtcggcta tgacgtcaag gacatagcgg aggtagcttt ctggctccac
     6841 tccgttcagt ttgcacgtcc cgatcaggct gtacagcagc gctccccgct ctcctccatg
     6901 atccgaaccg aagaacaggt agtttttgcg gcccagactg accatccgca acgcattttc
     6961 agcgatgtta ttgtccgcct cagcccagcc atcatctgca tagtacgtca gcgccggcca
     7021 ctggttcagg gcgtatgcga acgctttcgc cagttctgag tgtcgcgaca gggttttcat
     7081 cttttcacgc agccagcttt ccagggattt cagcagcggt ttcgttttca actgacgttc
     7141 ggcaaggcgc tgttccgccg tcattcccct tatctctgcc tcgatggcgt acagttcgcc
     7201 gatccgtttc agcgcttcct ccgtcagggc tgacggggtg cgaacgtgca catcgtggat
     7261 tttacggcgg gcgtgagccc aacaggcggc ttccgttatc cggccatccc ggtacagctc
     7321 gttgaacccg gcgtatgcat ccgcctgcag tacaccactg aaccccgcaa gatgggtctg
     7381 cggatggatg ccttttctgt ccgggctgta agcgaaccac accgccggcg ccagcgttga
     7441 cccggcgtta cggtcgtcac gaacatacgc ccacaaccgc ccggtcttcg ttttcttatt
     7501 gcctggcaac agcaccggga caggcgtgtc atcaacatgg agcttaccgt cagtcagcac
     7561 atagccatga agcgcctctt ccatcggaga cagcagccgg cagcatgcat ccacccagcc
     7621 cgacagcagt gaacggctca gctccacacc ttgccggccg tatatttctg actggcgata
     7681 cagcggggtg tgctctgcat acttcgaggt cagcacgcgg gccagcagcc ccggtccggc
     7741 gataccccgc tcgatgggcc gcgaaggtgc aggtgcctgc acgatggcat cgcactgagt
     7801 acaggcatgt ttttcccgta ccgtccggat aacccggaag gcgctacgca tcaactccag
     7861 ctgttcggcg gtatcctcgc ccagatagct cagtgaaccg ccgcagttcg ggcagcacgg
     7921 cgccgcaggc aacagtcgct tttcgtcacg gggtagtgat tcagggaacg gcttacgggt
     7981 gcgggtctga cgcaacggac gctgtactgc cgggtcatac accctaccag tcagcgtatc
     8041 gctctctttc tgaagccggt tcagatcggc ttccatttgt gcgatacggc gggagacttt
     8101 ttcggaacga ctgccgaagt tcatccggcg gagtttatcc agctgcgcct gcagatggtc
     8161 tatttcgcgc tcccggttgc taagcttttc ctgcagggcg tggatcagcg cttcctgttc
     8221 ggccaggcgc tgtttcagca ggaagatgtc gtcagaagag atgtcgttca taagcccgta
     8281 ttttaccggg cttattctgt gacaaccagg ataaagagat ttacagcatg gtcagggagg
     8341 tcagcagccg cttaggctgt cgccagtcga taccttccag cagcatcgcc agctgcgcct
     8401 gtgtaaggaa cactttgcca tcacgggctg acggccaggc gaagcgccca cgctccagcc
     8461 gtttggtcag gaggcacagt ccgtcactgg tggaccacag cagtttaacc tgactgccgc
     8521 tgcggccccg gaaaatgaaa acatggccgg acatgggatc gtctttcagc gccgtctgta
     8581 ctttcgcagc caggccgttg aagccatttc tcatatcggt gataccggca accagccaaa
     8641 ttttgatccc ggaaggtaac gggatcatcg cttcagttcc tgtatcagca gagtcaggag
     8701 cttttcgctg acattgccat tgaagcggag cgtcccgtgc cggaacgtta cctcacagct
     8761 gatactgagg gttttcgggt cctctgcgag cgattctggc tgttcggcag ctgcatcgag
     8821 agtcacagga agtagctggg ggctctctga agaaggtaat a
//
LOCUS       DAJTYZ010000152         8800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000152 DAJTYZ010000000
VERSION     DAJTYZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.152"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..766)
                     /locus_tag="OO596_003537"
                     /pseudo
     CDS             complement(1..766)
                     /locus_tag="OO596_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            1045..1998
                     /gene="tal"
                     /locus_tag="OO596_003538"
     CDS             1045..1998
                     /gene="tal"
                     /locus_tag="OO596_003538"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6719305.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2113..2700
                     /gene="mog"
                     /locus_tag="OO596_003539"
     CDS             2113..2700
                     /gene="mog"
                     /locus_tag="OO596_003539"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6719306.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2735..3301)
                     /gene="satP"
                     /locus_tag="OO596_003540"
     CDS             complement(2735..3301)
                     /gene="satP"
                     /locus_tag="OO596_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6719307.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="OO596_003541"
     CDS             complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="OO596_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6719308.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            complement(4189..4593)
                     /locus_tag="OO596_003542"
     CDS             complement(4189..4593)
                     /locus_tag="OO596_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6719309.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4970..6886
                     /gene="dnaK"
                     /locus_tag="OO596_003543"
     CDS             4970..6886
                     /gene="dnaK"
                     /locus_tag="OO596_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6719310.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6975..8105
                     /gene="dnaJ"
                     /locus_tag="OO596_003544"
     CDS             6975..8105
                     /gene="dnaJ"
                     /locus_tag="OO596_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6719311.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(8209..8361)
                     /gene="mokC"
                     /locus_tag="OO596_003545"
     CDS             complement(8209..8361)
                     /gene="mokC"
                     /locus_tag="OO596_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6719312.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2333 a         2184 c         2257 g         2026 t
ORIGIN      
        1 aaggtatatc ccgccgcgcc gcctgccgct tcctgccagc caaaagcact ctcaaaaata
       61 gaccaaatga cgtggggaag ttgcccgata tttattacgc aaatcaccat gctggtcagc
      121 acccagatta tcgccatcaa cgggacaaac ccctgcatga gccgggcgac gccatgaaga
      181 ccgcgagtga ttgccagcag agcaaagaca gcgagaataa tgcctgtcac cagcggggga
      241 aaatcaaaag aaaactcagg gctcgggcaa cggcgttcgc ctgaactccg ctgaaaatta
      301 tgccgtaggc gatgagcaaa aagacggcga acagaacgcc catccaacgc atccccagcc
      361 cgcgcgccat ataccatgcc ggtcctccac gaaactgtcc attgacgtca cgttctttat
      421 aaagttgtgc aagggaacat tcggcaaacg aggtcgccat gccgataaac gcggcaaccc
      481 acatccaaaa gacggctcca ggtccaccgg cggtaatagc cagcgcaacg ccggccaggt
      541 tgccgctacc cacgcgcgcc gcaagactgg tacacaatga ctgaaatgag gttaaaccgc
      601 ctggctgtgg atgaatgcta tttttaagac ttttgccaaa ctggcggatg tagcgaaact
      661 gcacaaatcc ggtgcgaaaa gtgaaccaac aacctgcgcc gaagagcagg taaatcatta
      721 ccgatcccca aaggacgctg ttaataaagg agaaaaaatc tggcatgcat atccctctta
      781 ttgccggtcg cgatgacttt cctgtgtaaa cgttaccaat tgtttaagaa gtatatacgc
      841 tacgaggtac ttgataactt ctgcgtagca tacatgaggt tttgtataaa aatggcgggc
      901 gatatcaacg cagtgtcaga aatccgaaac agtctcgcct ggcgataacc gtcttgtcgg
      961 cggttgcgct gacgttgcgt cgtgatatca tcagggcaga ccggttacat ccccctaaca
     1021 agctgtttaa agagaaatac tatcatgacg gacaaattga cctcccttcg tcagtacacc
     1081 accgtagtgg ccgacactgg ggacatcgcg gcaatgaagc tgtatcaacc gcaggatgcc
     1141 acaaccaacc cttctctcat tcttaacgca gcgcagattc cggaataccg taagttgatt
     1201 gatgatgctg tcgcctgggc gaaacagcag agcaacgatc gcgcgcagca gatcgtggac
     1261 gcgaccgaca aactggcagt aaatattggt ctggaaatcc tgaaactggt tccgggccgt
     1321 atctcaactg aagttgatgc gcgtctttcc tatgacaccg aagcgtcaat tgcgaaagca
     1381 aaacgcctga tcaaactcta caacgatgct ggtattagca acgatcgtat tctgatcaaa
     1441 ctggcttcta cttggcaggg tatccgtgct gcagaacagc tggaaaaaga aggcatcaac
     1501 tgtaacctga ccctgctgtt ctccttcgct caggctcgtg cttgtgcgga agcaggcgtg
     1561 ttcctgatct cgccatttgt tggccgtatt cttgactggt acaaagcgaa tactgataag
     1621 aaagagtacg ctccggcaga agatccgggc gtggtttctg tatctgaaat ctaccagtac
     1681 tacaaagagc acggttatga aaccgtggtt atgggcgcaa gcttccgtaa catcggcgaa
     1741 attctggaac tggcaggctg cgaccgtctt accatcgcac cggcactgct gaaagagctg
     1801 gctgagagcg aaggggctat cgaacgtaaa ctgtcttaca ccggcgaagt gaaagcgcgt
     1861 ccggcgcgta tcactgagtc cgagttcctg tggcagcaca accaggatcc aatggcagta
     1921 gataaactgg cggaaggtat ccgtaagttt gctattgacc aggaaaaact ggaaaaaatg
     1981 atcggcgatc tgctgtaatc attcttagcg tgaccgggaa gtcggtcacg ctacctcttc
     2041 tgaagcctgt ctgtcactcc cttcgcagtg tatcattctg tttaacgaga ctgtttaaac
     2101 ggaaaaatct tgatgaatac tttacgtatt ggcttagttt ccatctctga tcgcgcatcc
     2161 agcggcgttt atcaggataa aggcatccct gcgctggaag aatggctgac atcggcgcta
     2221 accacgccgt ttgaactgga aacccgctta atccccgatg agcaggcgat catcgagcaa
     2281 acgttgtgtg agctagtgga tgaaatgagt tgccatctgg tgctcaccac gggcggaact
     2341 ggcccggcgc gtcgtgacgt aacgcccgat gcgacgctgg cagtagcgga ccgcgagatg
     2401 cctggctttg gtgaacagat gcgccagata agcctgcatt ttgtaccaac tgcgatcctt
     2461 tcgcgtcagg ttggtgtgat tcgcaaacag gcgctgatcc ttaacttacc cggtcagccg
     2521 aagtctatta aagagacgct ggaaggtgtg aaggacgctg cgggtaacgt tgtggtacac
     2581 ggtatttttg ccagcgtacc gtactgcatt cagttgctgg aagggccata cgttgaaacg
     2641 gcaccagaag tggttgctgc attcagaccg aagagtgcaa gacgcgacgt tagcgaataa
     2701 aaaaatcccc ccgagcgggg ggatctcaaa acaattagtg ggattcacca atcggcagaa
     2761 cggtgcgacc aaactgctcg ttcagtactt cacccatcgc cagatagatt gcactggcac
     2821 cacagatcag accaatccag ccggcaaagt ggatgattgc ggcgttaccg gcaatgttac
     2881 cgatcgccag cagggcaaac agcacagtca ggctaaagaa aacgaattgc agaacgcgtg
     2941 cgcctttcag cgtgccgaag aacataaaca gcgtaaatac gccccacaga cccaggtaga
     3001 caccaaggaa ctgtgcattt ggcgcatcgg tcagacccag tttcggcatc agcagaatcg
     3061 caaccagcgt caaccagaaa gaaccgtaag aggtgaatgc ggttaaaccg aaagtgttgc
     3121 cttttttgta ctccagcaga ccggcaaaaa tttgcgcgat gccgccgtag aaaatgccca
     3181 tggcaagaat aataccgtcc agagcgaaat aacccacgtt gtgcaggtta agcagaatgg
     3241 tggtcatgcc gaagcccatc aggcccagcg gtgccggatt agccaactta gtgttgccca
     3301 taattcctca aaaatcatca tcgaatgaat ggtgaaataa tttccctgaa taacagtagt
     3361 gttttcaggg cgcggcataa taatcagcca gtggggcagt gtctacgatc ttttgagggg
     3421 aaaatgaaaa ttttccccgg tttacggtgt cagacctgag tggcgctaac catccggcgc
     3481 aggcaggcga tttgtattac ggctggaatt gtcacgcgat aggcactgcc gctgaccgct
     3541 ttaaccccat ttaatgctgc gcccacaggg cctcccagac ccgcgccgcg cagcaaacca
     3601 tgcccaagca cgctcattgc tgcgtgggtg cgtaaaatgc gggtcaattg gctggaaagc
     3661 aaatgcgaca cgccttttgc caataatttg tctttcatca gcagcggcag cagctcttcc
     3721 agctcattca ccctggcatc gaccgcgtgc agaaactcct gcttatgttc ctcgtccata
     3781 ttcttccagg tgttacgcag aaattgttcc agtaactgtt gctcaatctc aaacgtagac
     3841 atctctttgt cggctttcag cttcaatcgt tttgaaacat cgagcaaaat ggcccgatac
     3901 aatttaccgt gtccgcgcag tttgttggcg atactatcgc caccaaaatg ctgtaattct
     3961 ccagcaatca gctgccagtt gcggcgatgt tgctcgggat gcccttccat cgatttaaac
     4021 agttcgttgc gcatcagtac gctggagagg cgagttttgc ctttttcatt atgggtgagc
     4081 aatcgggcga aatttgccaa ctgttcctca ctacaatgct gaagaaaatc cagatctgaa
     4141 tcattcaggt aattaacatt cattttttgt ggtttctata ttctggcgtt agtcgtcgcc
     4201 gataattttc agcgtggcca tatccgatga gttcaccgta tgaccggaaa aggtgatttt
     4261 tgagacgcag cgtttattgt cgttatcact gttaatgttg atccagtcag tggtttgccc
     4321 ttcttttatt tctgaaggaa tattcagact ctgactggcg ctacgggcgg ctttgaaata
     4381 aaccgatgca ccgcttaact gtaaatcgcc atggtcggca gagagttgta tgcgtttcac
     4441 aatgcgacaa acaggaagtt tcagcgccag atcgttggtt tcgttacgcg gcattgcaat
     4501 gacgccgagg agtttatggt cgtttgcctg cgccgtgcag cacagcatca ggctaatcgc
     4561 caggctggcg gaaatcgtaa aaacggattt cataaggatt ctcttagtgg gaagaggtag
     4621 agggatgaat acccactagt ttactgctga taaagagaag attcaggtac gtaatctttt
     4681 ctttttatta caattttttg atgaatgcct tggctgcgat tcattcttta tatgaataaa
     4741 attgctgtca attttacgtc ttgtcctgcc atatcgcgaa atttctgcgc aaaagcacaa
     4801 aaaatttttg catctccccc ttgatgacgt gggttacgac cccatttagt agtcaaccgc
     4861 agtgagtgag tctgcaaaaa aatgaaattg ggcagttgaa accagacgtt tcgcccctat
     4921 tacagactca caaccacatg atgaccgaat atatagtgga gacgtttaga tgggtaaaat
     4981 aattggtatc gacctgggta ctaccaactc ttgtgtagcg attatggatg gcaccactcc
     5041 tcgcgtgctg gagaacgccg aaggcgatcg caccacgcct tctattattg cctataccca
     5101 ggatggtgaa actctggttg gtcagccggc taaacgtcag gcagtgacga acccgcaaaa
     5161 caccctgttt gcgattaaac gcctgattgg ccgccgcttc caggacgaag aagtacagcg
     5221 tgatgtttcc atcatgccgt tcaaaattat tgctgctgat aacggcgacg catgggtcga
     5281 agttaaaggc cagaaaatgg caccgccgca gatttctgct gaagtgctga aaaaaatgaa
     5341 gaaaaccgct gaagattact tgggtgaacc ggtaactgaa gctgttatca ccgtaccggc
     5401 atactttaac gatgctcagc gtcaggcaac caaagacgca ggccgtatcg ctggtctgga
     5461 agtaaaacgt atcatcaacg aaccgaccgc agctgcgctg gcttacggtc tggacaaagg
     5521 tactggcaac cgtactatcg cggtttatga cctgggtggt ggtactttcg atatttctat
     5581 tatcgaaatc gacgaagttg acggcgaaaa aaccttcgaa gttctggcta ccaacggtga
     5641 tacccacctg gggggtgaag acttcgacag ccgtctgatc aactacctgg ttgaagaatt
     5701 caagaaagat cagggcattg acctgcgcaa cgatccgctg gcaatgcagc gcctgaaaga
     5761 agcggcagaa aaagcgaaaa tcgaactgtc ttccgctcag cagaccgacg ttaacctgcc
     5821 gtacatcact gcagatgcga ccggtccgaa acacatgaac atcaaagtga ctcgtgcgaa
     5881 actggaaagc ctggttgaag atctggtaaa ccgttccatt gagccgctga aagttgcact
     5941 gcaggacgct ggcctgtccg tatctgatat cgacgacgtt atcctcgtgg gtggtcagac
     6001 tcgtatgcca atggttcaga agaaagttgc tgagttcttt ggtaaagagc cgcgtaaaga
     6061 cgttaacccg gacgaagctg tagcaatcgg tgctgctgtt cagggtggtg ttctgactgg
     6121 tgacgtaaaa gacgtactgc tgctggacgt taccccgctg tctctgggta tcgaaaccat
     6181 gggcggtgtg atgacgacgc tgatcgcgaa aaataccact atcccgacca agcacagcca
     6241 ggtgttctct accgctgaag acaaccagtc tgcggtaacc atccatgtgc tgcagggtga
     6301 acgtaaacgt gcagctgata acaaatctct gggtcagttc aacctggatg gtatcaaccc
     6361 ggcaccgcgc ggcatgccgc agatcgaagt taccttcgat atcgatgctg acggtatctt
     6421 gcacgtttcc gcgaaagata aaaacagcgg taaagagcag aagatcacca tcaaggcttc
     6481 ttctggtctg aacgaagatg aaatccagaa aatggtacgc gacgcagaag ctaacgccga
     6541 agctgaccgt aagtttgaag agctggtaca gactcgcaac cagggcgacc atctgctgca
     6601 cagcacccgt aagcaggttg aagaagcagg cgacaaactg ccggctgacg acaaaactgc
     6661 tatcgagtct gcgctgactg cactggaaac tgctctgaaa ggtgaagaca aagccgctat
     6721 cgaagcgaaa atgcaggaac tggcacaggt ttcccagaaa ctgatggaaa tcgcccagca
     6781 gcaacatgcc cagcagcaga ctgccggtgc tgatgcttct gcaaacaacg cgaaagatga
     6841 cgatgttgtc gacgctgaat ttgaagaagt caaagacaaa aaataatcgc cctataaacg
     6901 ggtaattata ctgacacggg cgaaggggaa tttcctctcc gcccgtgcat tcatctaggg
     6961 acaatttaaa aaagatggct aagcaagatt attacgagat tttaggcgtt tccaaaacag
     7021 cggaagagcg tgaaatcaaa aaggcctaca aacgcctggc catgaaatac cacccggacc
     7081 gtaaccaggg tgacaaagag gccgaggcga aatttaaaga gatcaaggaa gcttatgaag
     7141 ttctgaccga ctcgcaaaaa cgtgcggcat acgatcagta tggtcatgct gcgtttgagc
     7201 aaggtggcat gggcggcggc ggttttggcg gcggcgcaga cttcagcgat atttttggtg
     7261 acgttttcgg cgatattttt ggcggcggac gtggtcgtca acgtgcggcg cgcggtgctg
     7321 atttacgcta taacatggag ctcaccctcg aagaagctgt acgtggcgtg accaaagaga
     7381 tccgcattcc gactctggaa gagtgtgacg tttgccacgg tagcggtgca aaaccaggta
     7441 cacagccgca gacctgtccg acctgtcatg gttctggtca ggtgcagatg cgccagggat
     7501 ttttcgctgt acagcagacc tgtccacact gtcagggccg cggtacgctg atcaaagatc
     7561 cgtgcaacaa atgtcatggt catggtcgtg ttgagcgcag caaaacgctg tccgttaaaa
     7621 tcccggcagg ggtggacact ggagaccgca tccgtcttgc gggcgaaggt gaagcgggcg
     7681 agcatggcgc accggcaggc gatctgtacg ttcaggttca ggttaaacag cacccgattt
     7741 tcgagcgtga aggcaacaac ctctattgcg aagtcccgat caacttcgct atggcggcgc
     7801 tgggtggcga aatcgaagta ccgacccttg atggtcgcgt caaactgaaa gtgcctggcg
     7861 aaacccagac cggtaagctt ttccgtatgc gcggtaaagg cgtcaagtct gtccgcggtg
     7921 gcgcacaggg tgatttgctg tgccgcgttg tcgtcgaaac accggtaggc ctgaacgaga
     7981 agcagaaaca gttgctgcaa gagctgcaag aaagcttcgg tggcccaacc ggtgagcaca
     8041 acagcccgcg ctcaaagagc ttctttgatg gtgtgaagaa gttttttgac gacctgaccc
     8101 gctaacctcc ccaaaagcct gcccgtgggc aggcttgggt aaaattaggg tgcgttgaag
     8161 atatgcgagc acctgaaaag tggcggggat cactccccgc cgttgctctt actcggattc
     8221 gtaagccgtg aaaacagcaa cctccgtctg gccagttcgg atgtgaacct cacagaggtc
     8281 ttttctcgtt accagcgccg ccactacggc ggtgatacag atgacgatca gggcgacaat
     8341 catcgcctta tgctgcttca ttgctctctt ctccttgacc ttacggtcag taagaggcac
     8401 tctacatgtg ttcagcatat agggggcctc gggttgatag taaaatatca ctcggggctt
     8461 ttctctatct gccgttcagc taatgcctga gacagatagc ctcaagcacc cgccgctatt
     8521 atatcgctct ctttaaccca ttctgtttta tcgattctaa tcctgaagac gcctcgccta
     8581 tttttgtggc gtaatttttt aatgatttaa ttatttaact ttaatttatc tatttatcgc
     8641 aattattgac gacaagctgg attatttttg aaatattgtc ctaacaaata acgccgactg
     8701 acaagaaatt aattattact tttcctaatt aatccctcag aaatcctgac cttaagctat
     8761 gattatcagg gcttagggtc acccgttagc gctgatatga
//
LOCUS       DAJTYZ010000153         8634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000153 DAJTYZ010000000
VERSION     DAJTYZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.153"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="OO596_003546"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="OO596_003546"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6719313.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="OO596_003547"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="OO596_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6719314.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="OO596_003548"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="OO596_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6719315.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="OO596_003549"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="OO596_003549"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6719316.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="OO596_003550"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="OO596_003550"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6719317.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /locus_tag="OO596_003551"
     CDS             complement(7825..8136)
                     /locus_tag="OO596_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6719318.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="OO596_003552"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="OO596_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6719319.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2247 c         2051 g         2180 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taat
//
LOCUS       DAJTYZ010000154         8535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000154 DAJTYZ010000000
VERSION     DAJTYZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.154"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            814..1056
                     /locus_tag="OO596_003553"
     CDS             814..1056
                     /locus_tag="OO596_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719320.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1386..2315)
                     /gene="fdhE"
                     /locus_tag="OO596_003554"
     CDS             complement(1386..2315)
                     /gene="fdhE"
                     /locus_tag="OO596_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6719321.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2312..2947)
                     /gene="fdoI"
                     /locus_tag="OO596_003555"
     CDS             complement(2312..2947)
                     /gene="fdoI"
                     /locus_tag="OO596_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6719322.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2944..3846)
                     /gene="fdoH"
                     /locus_tag="OO596_003556"
     CDS             complement(2944..3846)
                     /gene="fdoH"
                     /locus_tag="OO596_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6719323.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3859..6909)
                     /gene="fdnG"
                     /locus_tag="OO596_003557"
     CDS             complement(3859..6909)
                     /gene="fdnG"
                     /locus_tag="OO596_003557"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6322..6324),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6719324.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7103..7936
                     /gene="fdhD"
                     /locus_tag="OO596_003558"
     CDS             7103..7936
                     /gene="fdhD"
                     /locus_tag="OO596_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6719325.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            8089..>8535
                     /locus_tag="OO596_003559"
     CDS             8089..>8535
                     /locus_tag="OO596_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR6719326.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVS"
BASE COUNT         2009 a         2178 c         2201 g         2147 t
ORIGIN      
        1 tcatttacat cattccctgt ctatgccggt aatattgaat aagaactcta taaattttta
       61 ttcttcgttg gcaccatacg tgtcttgcta catcactgtt caggagcatt taaatattat
      121 ttgtagagct gagtggcaat tatagcagat aatgcttaac tcatcatctt agttcaggtt
      181 tgagcgattt ttgatagtca aaacctcctc tggtgccata cttttctcat aaatattttt
      241 aaaatctata gctcacaaac attatgcaaa gataatgggc tggcatcatt gtcagtatca
      301 ttcatttact gctctgtatg ttatgaatca ttatgttgta tgtttgctct taaaccgtgg
      361 gaaggaggct ataatggttg ccaatattaa tctcattaaa caagaaagtt attctgttgt
      421 taatttagaa aaacaacttt cccgaactgt taccaacaaa attataacga tgagcaagta
      481 aagaccggtt ggtggcagta ctgaccggtc tgtgatgaaa gattaatttc accttaaatc
      541 ctggtgctgt taggatgctg acgtttttcg gctacaccat tcagattatg caaataaata
      601 tccctgaata taatatattc atagcaccca taactcatcc tgggcgataa ctaaaccatt
      661 tttgagtaat aaatgaaaca actgcaacgc tcttattatt tctggaaaac gcctcgcaaa
      721 aataaaaaat gatgcgtaaa tgagctttct ttaaagattc ggcaagggta cttttctctc
      781 catagattca aaaatgatat tggcgagata tttatgaact ctctggcagg tatagatatg
      841 ggcagaattt tactcgattt atcgaatgag gtgattaagc aacttgatga tctcgaggtg
      901 cagcgtaatc ttcctcgtgc ggacctatta agggaagcag tagatcaata cctgataaat
      961 caatcgcaaa cagcaagaac cagtgttcct ggcatctggc aagggtgtga ggaagatggt
     1021 gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg aaaaaatgat gtcaatcaga
     1081 ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt gaacgcctta tccggcctac
     1141 aaagtcatgc aaattcaata tattgaagtt tattggtagg cctgataagc gcagcgcatc
     1201 aggcaatcat acgcttgtca tcagtgacat acccataacc ttatcgatgt gttggaggga
     1261 agaacgaggc aaacagacaa ttgcggcatg atcttgaatg ggtaacgcgg aatacgagag
     1321 atggtgtcgt cacaccctat gagctgtaat ccgggcgacc tgcgccaccc ggaaaacgtc
     1381 agagattact ctccttcacc cggaaacagg aacgggttaa tggaactacg ggcgtagcct
     1441 tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt catctgccac ggcttcaact
     1501 ttcggttctt tttcctgata aagaatcttc aggtaagtgc cgcagtcatc gcagctttcg
     1561 gctttaatcg cggcctgttc gtcatccagc gaccagtaat gcagtttgcc gctctgctcg
     1621 cagttgctgc attttacgcg cactacgtgc cattcggttt cacacaggtt gcagtgcagg
     1681 taacgcagac cctgagtggt gccaatttgc accatgctgg acaccggcat agagccacat
     1741 accgggcaat attgacgttg ttcgccgtat tcagcgcggg ctttgccggg gatcagattg
     1801 gccatctgcg cccagtagag cgacagcgca gcccagataa acggcgcttt atcgctgctg
     1861 acggacgaga aatcagaggc aaacagcgcg ctggccatat cttccagctc ctgagtcgat
     1921 gccttctcca gattctcaat cactgccagc gccgggccgc tcatttcagg tttcagctca
     1981 gcaattagcg ccatcagcag cttttgccag tgcttatcac gcggcagaac gtgaatatcc
     2041 agcgggggct tgccctgtgc gctggcttct ttaatgcgcg cggtcagatc catctccagc
     2101 ggatggtcgt acagcaccac ttcctgggcg tgggcgataa gcgcagcaaa gcgcaggtaa
     2161 tcacccagcg gattattttc tgccagctcg cgcagacgct cggcgcggcg gttgtataaa
     2221 ttcttgagcc gagggaacaa taacggcgga atcatatccg ccgtacgttt ctcgctcgaa
     2281 cccagctcat cttgcgggat tatgcgaata ctcattcagc tttcttttcc gttgtcttgc
     2341 ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca ggcgctggta acccatcctt
     2401 ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc gtagatatgc accatgataa
     2461 ccacaattaa cgccactgcg gcaaatgaat gcagcattaa cgcgaatcgg atcaccggga
     2521 ttgagaaagc aggcgcaaaa taaggacgcc agataatcac gccgctcacc agcagcagaa
     2581 ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc gaagttataa cgcccggtgt
     2641 cacctacttc ctcgttgacg acgatcttac gaatattctt cgcccaaaag atatcatccc
     2701 gattgattag gttgtgatgc cagtaacgga aaaacatgat gatgaacgag gcaaacataa
     2761 ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg tgtgcccatg atttgcatca
     2821 accagttgaa ggacgggaac aaaaagccca gcccgctcac cgccgccagg atgaagcaga
     2881 aggcggtgat ccagtggttg atacgttccg gcgcggtgta gcgcacgatg gtgtcacgtc
     2941 gtttcatttg cgctcctcgt ctttctcttc gtgcagatta ttctcttcct catccgcacg
     3001 gttcggaccg acacccacgt agtggaagat actggccgca aaggtagccg caaagccaac
     3061 agccgcgagc ggtttccaga tgcctttcca gaatttcacg gtttcgctga tttccgggtt
     3121 ctccggcaag ccatgataca gatttggctt gtcagcatgg tgcagcacgt acatgacgtg
     3181 tgtaccaccg acgcctgccg gatcgtacag acccgcattg tcgtaaccgc gggttttcag
     3241 ctcagcaacg cgctcgctcg ccagcgtttt catcgactct ttcgtaccga agtgaatcgc
     3301 gcccgttggg caggtcttca cgcaggctgg ttcttgccca accaccacgc ggtcaacgca
     3361 cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg cgcggaatgt cgaacggaca
     3421 gcccgcaatg caataaccgc agccaatgca ctgctcggac tggaagtcga cgataccgtt
     3481 ggcatactga ataattgcac cttccgccgg gcacgctttc aggcagcctg gatcggaaca
     3541 gtgcatacag ccgtctttgc ggatcagcca ttccagtttg tcgttctgct ccacttccga
     3601 gaagcgcatc accgtccacg atttggcgct taaatcattg gggttgtcgt acaccccaat
     3661 gttattgccg acggtatcgc gaatgtcgtt ccactctgaa cacgccacct gacaggcttt
     3721 acagccgata caggtggtga cgtcgatgag tttcgccact tcttcctgga agtcccgcgc
     3781 ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga atgatatctt gcgattgata
     3841 agccatatgt cgtctccgtt acaccttttc cacattcaca aggaaggact taaactccgg
     3901 cgtctgcgtg ttcgcatcac caacgaatgg cgttaacgta ttggcaataa agcctttttt
     3961 agctacgcct tcatagcccc agtgaatcgg aataccgatg gtatcgatat ctttgccgtt
     4021 cgctttcagc gtgcgaatac gtttggtcac caccgctttg gctttgatat agccacggtt
     4081 ggaggagact ttcacggtat cgccctgggc aatgccaagt ttattcgcca gcgactcccc
     4141 gatttccaca aactgctctg gttgcaaaat cgcgttcaac agcgcgtgtt tggtccagta
     4201 gtggaagtgc tcggtcagac gataggtggt tccgacatac gggaacttat cggctttacc
     4261 caatgcttcg gcgtcgtctt taaagattcg cgcagccggg ttcgagataa cgtttggatg
     4321 cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag tgttccggga acggaccttc
     4381 cgccatctta tcgagggcaa acagacgccc catgccttcc tgctgcatga taaacggccc
     4441 gacgccgctg cccggaggcg ctgcgctgta gtccggaata tcccagccgg tccacttagt
     4501 gccgtcccat ttcagcaact gacgcttcgg atcccacggg ttaccctgcg gatctgcgga
     4561 ggcgcggtta tacagaatgc ggcggttaag cggccatgcc catgcccagc ccagcgtgtt
     4621 accgaggcca gacggatcgg cgttatcacg acgcgccatc tggttgcctt ccggcgtcca
     4681 gctaccggcg aaaatccagc agccacagga ggttgtaccg tcatcgcgca attgggcgaa
     4741 cgagctaagt tgttggcctt tcttgacgat aaccgcaccg gttgccggat cggtaatatc
     4801 ggccagcgcc ttaccgttgc tctccatcgc cacttcttcc gacttcggct catgcggaat
     4861 ggcgtagttc caggtcatgt tcagcacctg atccgggttt gcgccaccct gttcggcata
     4921 catcttgcgc aagcgcagga agataccgga gaggatctcg ccatcagtca gcgcaatccc
     4981 tggggcgtcc gcacctttcc agtgccactg caaccagcga cctgagttaa cgattgaacc
     5041 gttctcttct gcgaagcagg tcgatggcag acggaacact tcggtctgga tcttcgacga
     5101 gtcaacttcg ttcagctcac cgtggttctg ccagaagtta gaggtttcag tgttcagcgg
     5161 gtcgatggtg acgaggaact tcagtttcga cagacaaccg atcactttgt ttttgttcgg
     5221 gaatgaggca acagggttaa agccctggca gatatagcca ttgaccttgc cctctttcat
     5281 catctcgaag tattgcagga cgtcgtagcc tttatcccac ttcggcaacc agtcaaagcc
     5341 ccagctattt tccgccgtcg ctttatcacc aaagaaggcc ttcatcatag agacgaagaa
     5401 tttcgggtag ttgccccagt agtttacctg gccttccagc agcggttttg gcgtgttggc
     5461 ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc agcgtcatgt aacctggcag
     5521 gctctgcgac agcagcccca ggtccgtcag cccctgaata ttggagtgac cgcgcagggc
     5581 gttaacgccg ccgcctgcca tccccatatt gccgagcagc agctggatca tcgccatcgt
     5641 acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg agggcataca ggaacgaggc
     5701 agttttatcg tgagcactgg tttctgcgat gtattcgcag actttcagga acgcgtcttt
     5761 tggcgtacca cagatgtttt caaccacatc tggcgtgtag cgggaaacgt gctgtttcag
     5821 caagttccac acgcagcgcg gatgttgcag cgtggtatcg cgtttggcga agccgttttc
     5881 gtccagttcg taagtccagg aggatttatc gtacttgcgt ttttccgcgt catagccggt
     5941 gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc aggctggcgt tggtataggc
     6001 ttcggtgtat tcgcggttga atttttcatt gttcagcagg tacagcaata cgcctgacag
     6061 gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag tcagccaccg ccgccgtacg
     6121 cgtaaagcga ggatcgatca caatcagctt cgcgccattg tgaattttgg cttccatcgc
     6181 ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg cccatcacca cgacgaggtt
     6241 ggcgttcttg atgtcgaccc agtggttggt catcgcaccg cgaccaaatg ttggagcaag
     6301 acttgctacc gttggtccgt gtcagacacg cgcctggttg tcgaccgcga gcatacccag
     6361 cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta ctcgacgcgg aagcacacag
     6421 catcccggtg gagagccagc ggttaacagt cacgccttcg gcgttttgcg caatgtagtt
     6481 agcatcgcgg tcttctttca tcagtttggc gatgcgatca aacgcctctt cccaactgat
     6541 ttgctgccat ttatcagaac ctggcgcacg gtattccgga aacttcagac ggctttcgga
     6601 gtggatgaaa tccaccaggc cagcgccttt cggacaaagt gcaccgcggt tgaccgggtg
     6661 atccggatcg ccttcgatat ggaagataga tgctttggcg ttttttgctc cgtcaccgag
     6721 gctgtacatc aacagcccac agcctacgga acaataggtg caggtattac gggtttcgcg
     6781 ggtgcgcagc agtttatact gccgggtttc cgcgagtgct acgctgggtg caaaacccag
     6841 tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc ttaaagaact gccttctgct
     6901 gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc ccatttacat tcgcttgctg
     6961 cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg gccgcaaagg tccagaattg
     7021 gctcaatttc cctccatcca ggaagggttt gtaacagaat accataatgt tggtgtgtgt
     7081 gttcttatct ggttaagaga aagtgaaaaa aacacagcga aaagaaatcg aaaatgtgac
     7141 aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt gatgatttac aacacccacg
     7201 gttagatgag gtcgcggaag aagttcccgt tgcgctggtc tacaacggca tttcgcatgt
     7261 ggtgatgatg gcgtcgccca aagaccttga gtactttgcg ctcggttttt cgctttccga
     7321 ggggataata gaaagtccgc gcgatatctt cggcatggat gtcgttcctt cctgtaatgg
     7381 tcttgaagta caaattgagc tttccagccg ccgctttatg gggttgaagg agcgccgccg
     7441 ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg gagcaactta atgacatcgg
     7501 aaaaccggtg cagccgctac cgttcaccca gacgtttgat ctcaacaaac tggatgatgc
     7561 attacgtcat ctcaacgatt ttcagccagt ggggcaactg actggttgta ctcacgccgc
     7621 tgcctggatg ttgccatctg gcgaactggt cggcgggcat gaagacgtgg gtcgccatgt
     7681 ggcgctggac aaactgttag gccgccggtc acaagaaggg gaaagctggc agcaaggtgc
     7741 ggtactggtt tccagtcgtg ccagttatga aatggtgcaa aagtcggcga tgtgcggcgt
     7801 agagattttg tttgcggtgt ctgccgcgac cacgcttgct gtagaagtgg ctgagcgctg
     7861 taatctgaca ctggtaggtt tttgtaaacc gggtcgggca acggtttata cccatccgca
     7921 gcgtttgagc aattaattaa aataagtcct gcgatatata ttaaattgca ggacttattc
     7981 atttcgtgaa ttttattatt ttatttataa ataaaacgcg tgtagtgacg ttttcttttt
     8041 tcatttatta tgttcactct cttatctata tatttataag gcaattaaat gaaaaggaat
     8101 ttattatctt ccgctattat agtcgccatc atggccctcg gtctgacggg ttgtgatgat
     8161 aaaaaagccg aaacagaaac gcccccgcct gccaatagtc aacctgccgc accagctcct
     8221 gaagctcccg ttgcaaaagc agaagctaaa cctgaaacac ctgcgcaacc ggtggtcgat
     8281 gaacaagcgg ttttcgacga aaaaatggat gtctatatca agtgctacaa caagttacag
     8341 atccctgtac agaacagtct ggcgcgttat gctgactggc tgaaagattt taaacagggg
     8401 cctaccggta aagagagtac tgtttatggc atctacggca ttagtgaatc caacctcgct
     8461 gagtgtgaaa aaggcgtaaa aagtgctgtg gcgttaacgc ctgcgctgca acctattgat
     8521 ggcgtggcgg tgagt
//
LOCUS       DAJTYZ010000155         8484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000155 DAJTYZ010000000
VERSION     DAJTYZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.155"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..395)
                     /gene="mscS"
                     /locus_tag="OO596_003560"
     CDS             complement(<1..395)
                     /gene="mscS"
                     /locus_tag="OO596_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6719327.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRA"
     gene            complement(753..1832)
                     /gene="fbaA"
                     /locus_tag="OO596_003561"
     CDS             complement(753..1832)
                     /gene="fbaA"
                     /locus_tag="OO596_003561"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6719328.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(2047..3210)
                     /gene="pgk"
                     /locus_tag="OO596_003562"
     CDS             complement(2047..3210)
                     /gene="pgk"
                     /locus_tag="OO596_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6719329.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(3260..4279)
                     /gene="epd"
                     /locus_tag="OO596_003563"
     CDS             complement(3260..4279)
                     /gene="epd"
                     /locus_tag="OO596_003563"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6719330.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            4651..5082
                     /locus_tag="OO596_003564"
     CDS             4651..5082
                     /locus_tag="OO596_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6719331.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            5105..5683
                     /locus_tag="OO596_003565"
     CDS             5105..5683
                     /locus_tag="OO596_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6719332.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            5684..6391
                     /locus_tag="OO596_003566"
     CDS             5684..6391
                     /locus_tag="OO596_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6719333.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            6379..7056
                     /locus_tag="OO596_003567"
     CDS             6379..7056
                     /locus_tag="OO596_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6719334.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            7050..7727
                     /locus_tag="OO596_003568"
     CDS             7050..7727
                     /locus_tag="OO596_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6719335.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(7699..8412)
                     /locus_tag="OO596_003569"
     CDS             complement(7699..8412)
                     /locus_tag="OO596_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6719336.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(8409..>8484)
                     /locus_tag="OO596_003570"
     CDS             complement(8409..>8484)
                     /locus_tag="OO596_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6719337.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         1959 a         2274 c         2168 g         2083 t
ORIGIN      
        1 gcacggaacg ggcggaacat gacaagtaac acgccagcgg ccaggttaga aagtgacccc
       61 tgcaaagcca gaccaacagc taagcctgcg gcaccgagta cagcaatgac tgacgctgtt
      121 tgtacaccca cgcgtcccag ggcagcgatt agcgtaaagg cgataatacc gtaacggact
      181 aatgcagaaa gaaaatcagc aacagtggca tcgattttac gggagatcat caggcgattc
      241 accgcgttgg aaatcatccg cgcgataatc aaaccaacga tgatgatcgc gagtgccgcc
      301 acgatgttta ctgcataact tagcagcagc gcctggttag ctaccagcca gcttcccgcg
      361 ccgtttatgc tatcgacaac attcaaatct tccattcaat attccttttc aactgactca
      421 aaatggagaa atacactccg ccgccttatg acgggcagtc tgacaagggt aaacaataaa
      481 gcataatttt gccaaataga tcacagattt ctcatctgaa tcaccttgct tttggcgaaa
      541 aaatccccgc agagcggggc tcagacagat atcaatgtgc gctttgtcat gccggatgcg
      601 gcgtgaacgc cttatccggc ctacaaaaac ctgcaaattc aatatattgc aggatacgtg
      661 taggcctgat aagcgtagcg catcaggcaa ttttgctttt ggcgaaaaaa agacccgcag
      721 agcgggcctt gagataagca gaaaggaata tcttacagaa cgtcgatcgc gttcagttcc
      781 tggaatgctt tttccagacg agcgatcatc gaagtctgac cggcacgcag ccatacgcgc
      841 ggatcgtagt atttcttgtt cggctgatct tcgcctttcg ggttacccaa ctgcccctgc
      901 agataagctt cgttcgcttt gtagtagttc agaacgcctt cccaggttgc ccattgggta
      961 tcggtatcga tgttcatttt tactacgccg tagcttacgg agtctttgat ttcctgagca
     1021 gtagaaccgg aaccaccgtg gaatacgaag ttcaggctgt tgtgcggcag gttgtgtttc
     1081 ttggaaacat attcctgaga atcacgcagg atggtcggag tcagaaccac gttacccggc
     1141 ttgtaaacac cgtgtacgtt accgaaggac gcagcgatgg tgaaacgcgg gctgattttg
     1201 ctcagttcgg tgtatgcgta atcaacgtct tccggctggg tgtacagtgc agaagcgtcc
     1261 atgtggctgt tgtccacgcc gtcttcttca ccaccggtgc aacccagttc gatttccaga
     1321 gtcatgccga ttttggacat gcgctccagg tatttagagc agatttcgat gttctcttgc
     1381 agagattctt cagacaggtc gatcatgtga gaagagaaca gcggcttacc ggtagctgcg
     1441 aagtgttttt cacccgcgtc cagcagaccg tcgatccacg gcagcagttt cttcgcgcag
     1501 tggtcagtgt gcaggataac cggaacacca taatgttcag ccatctggtg aacgtgatgc
     1561 gcaccagaga tcgcgcccag gatagcagca ccctgcggaa cgtcagattt cacgccttta
     1621 ccagcgataa aggaagcacc accgttggag aactgaacga taaccggcgc tttaacttta
     1681 gcagcggttt ccagtacggc gttgatggag tcagtaccga cgcagtttac tgctggcagt
     1741 gcgaagttgt tttcttttgc tacctggaaa actttctgta cgtcatcacc agtgattacg
     1801 ccaggtttta cgaaatcaaa aatcttagac atgtctcttg tcctgtatcg tcgggcctta
     1861 tactcatcat acttcaagtt gcatgtgctg cgtctgcgtt cgctcacccc agtcacttac
     1921 ttatgtaagc tcctggggat tcactctctt gtcgccttcc tgcaactcga attatttaga
     1981 gtatgaaaaa atgcgtgctc taaaagcgcg ctgaaacaag ggcaggtttc cctgccctgt
     2041 gattttttac ttcttagcgc gctcttcgag catcgctact gcaggcagta ctttaccttc
     2101 cacgaattcg aggaatgcgc cgccgccagt ggagatgtag gagattttgt cagcaatgcc
     2161 gaacaggtcg attgctgcca gagtgtcgcc gccgccagcg atggagaacg cttcgctgtc
     2221 tgcgatagcg ttagccacga tttcagtacc tttgcggaag ttcgggaatt cgaacacgcc
     2281 aaccggaccg ttccacagaa tggttttcgc attcttcagg atttcagcca gttcctgagc
     2341 ggaagcatca ccgatatcca ggatctgctc gtcagctttc acatcgttaa cagatttcag
     2401 ggtagccgat gcagtttcag agaactcggt tgctacgcga acatcagacg gaaccgggat
     2461 gttgcaggtg gtcagcagac gtttagcttc gtcaaccagg tcagcttcgt acagggattt
     2521 acccacatcg tggccttgtg ccgcgataaa ggtgttagcg ataccaccac caacaatcag
     2581 ctggtcagcg attttagaca gggagtccag aacggtcagt ttggtagata ctttagaacc
     2641 accaacgata gccaccatcg ggcgagcagg ttctttcagt gctttaccca gcgcgtccag
     2701 ttcagctgcc aacagcgggc ctgcgcacgc aacatcagcg aatttaccga taccgtgagt
     2761 agaagcctgc gcgcggtgag cagtaccgaa tgcgtccatt acgaacacgt cacacagtgc
     2821 agcgtatttt ttggacaggg tttcgtcgtc tttcttctcg cctttgttga agcgaacgtt
     2881 ttccagaaca accagttcac cttcagcaac gtcaacgccg tcgaggtaat ctttaaccag
     2941 acgaaccggg ttagacagtt tgtctttcag gtagttaaca accggcagca gagagaattc
     3001 ttcgttgtac tcgccttcgg taggacgacc caggtgggaa gttaccatca ctttcgcgcc
     3061 ttgtttcagg gccagttcga tggtcggcag agaagcacgg atacgcgcgt cgctggttac
     3121 tttcccgtct tttactggta cgttcagatc cgcacggata aatacacgtt tcccagcaag
     3181 atccagatcg gtcatcttaa ttacagacat ggtgaatcct ctcgttgatt ctaaaagttt
     3241 tgcagacgct gcttgcgtct tacctgaaag caacagtagc catagctaac gtcgtgtcga
     3301 gcattcggtt agcaaagccc cattcgttat cgcaccagac caacgttttg atcaggtgtg
     3361 cgccactgac ccgggtttgg gtgccatcga caatggcact gtgcggatcg tggttaaaat
     3421 ctacagagac caacggcaat tccgtatagt caactatacc atgaaatgca ccttgtgctg
     3481 ctttttgcag caacaggttg acttcattgg cttttacagg tttcttcacc gtcacgctta
     3541 aatcgattgc cgtcacattt atggttggca cacgtaccgc aatcgcttca aagcgatcgt
     3601 taaattgcgg aaaaaatcgt gtgataccgg cggccagttt agtatcgacc ggaatgatcg
     3661 actggctggc tgcccgggtg cgacgcaggt caggatggta tgcatcaata acctgttgat
     3721 cgtgcatggc ggagtgaatt gtggtcacag tgccggactc aataccgtac gcatcatcta
     3781 acaatttgat gacgggaatt atgcaattcg tggtacagga agcgttagaa acgatgcggt
     3841 gttccgcacg aagttgatcc tgattgacgc cgtaaacaac ggtcgcgtcg agatcgttac
     3901 tgccaggatg tgaaaagagc acttttttgg ccccggcggc aatatgcgct tcgccatgct
     3961 cgcgggagcc atatacgccg gtgcagtcga ggactacatc aacgccaagt tcacgccagg
     4021 ggagcgattg cagtgaacgt tcatgcaata cgcggatggc gtcatcacca acaaaaagtt
     4081 gatcgcgttc ctgtcgtact tcccatgcaa aacggccatg gctggtgtca tatttcaaca
     4141 aatgcgccat gcccgcagca tccgccagtt cgttgattgc caccacggta atttccgccc
     4201 ggcgtccgga ttcatacaaa gcacgaacca cattacgccc gatgcgaccg aagccattta
     4261 tcgctacgcg tacggtcata gatctcctgc aaggttttcc ctgagcaaat ttgccagaca
     4321 gagtaatcca gcaaatcgtc cggggaaacc ttacctgtcg caaactgcga ctgattggtt
     4381 aattgtcgaa catttaatcg actgaaacgc ttcagctagg ataagcgaaa cgtggaataa
     4441 aaggaatgat tgtccagtca aagaaaacaa ttatctgact tacgtcacgt tttcagcccc
     4501 gcccctgtgg aatttattcc actttgcaga atcacgaata agcgtagcaa aatcttttcg
     4561 ttgtgccgtc acaaaccctg acttatatca gaatatgact gatgcagcgt cgatattatc
     4621 cgcaatacgt ccgagtttcg gagtactcca atgatgacat tgccctaccc atattcttcc
     4681 cccgcacgct tttatgcact gcgcctcctc cccgggcagg aagttttttc ccagctacac
     4741 gcttttgcac agcagcatca acttcatgcc gcctggattg caggctgtac cggtagcctg
     4801 actgacgtcg ccttgcgcta tgccgggcaa gaaggcacca cgctccttaa cggtacattc
     4861 gaagtgatct cactaaacgg cacgctggaa cagagtggtg agcatcttca tctctgcgtt
     4921 tccgatccac acggcacgat gcttggcggt catatgatgc ccggctgtac cgtgcggacc
     4981 acgcttgaac tggtgatcgg tagccttgag gaactggcgt ttagccgcca accttgcgcg
     5041 ctttctggtt acgacgagtt gcatatttcc cccgttaaat aactactttc aaagaggttt
     5101 tcttatggcg cgtagccact tttccagtca ggcactggtg ttgatcgtta tatctatcgc
     5161 catcaatatg atcggcgggc aactggccag catggtcaaa ttacctatat ttctcgactc
     5221 catcggcaca cttattagcg cggtactgct tggtccggtg atcggaatgc tgaccggctt
     5281 actgaccaat ttactctggg gattgctgac cgacccgatc gccgccgcat ttgcacccgt
     5341 cgcgatggtc atcggcctgg tcgccggttg gctggcgcga gcgggttggt ttcgcaccct
     5401 gccaaaagtc gttgtcagcg gcgtgattat tacgcttgcg gtaacggtcg tcgctgtacc
     5461 gctgcgtact gcgctgttcg gcggtgtcac cggtagcgga gccgatttgt tcgttgcctg
     5521 gatgcactcg atggggcaaa acctggtgga gtcggtggcg attacagtga taggcgcgaa
     5581 cctggtcgat aaaattctta cagcggtgat tgtctggctt ttactgcgcc agctgccgat
     5641 tcgcaccacg cgccattttc cggcaatggc tgccgtgcgc taaatgcatc catttacctc
     5701 attaacactg tgggcattgg cggcctgcac cacgcttatc ctgcccgcgc agaccatttt
     5761 gccgatctat agcgcggcga cctttttttg tcttattgcg ctaaaggcca cgcggcggcg
     5821 ggcaaaatat gtcgcctggc tgatgttctc gctgggagcc gggctgtggc tggtacacgg
     5881 tggctggcta acagaatggc taagcggcac gccgcgttcg ccggagcgtt ggacgcatgc
     5941 tattacgctc tggttgcgga ttctggcgat tgtttcaacc tcacagttgt ggatgcagta
     6001 cgtccccgtg caatgtttta tccgcgctct gtttgcttct cgcctgccgc caggcgtcgc
     6061 ttatctgttt gccggaccgt tattggtcgt tgagcagtta aaacggcaat tagcgattat
     6121 tcatgaagcg cagcgtgccc gcggcgtgcc gctggatgag ggctggtatc agcgtttacg
     6181 tgcgatgccc gcgttaatta ttctgctaac acacaatgcg cttaacgatc tggctgtccg
     6241 tggtgcggcg ctggatatgc gggcatttcg gatccacaac cgacgaacaa cgttgtgggc
     6301 accagccgat agcaccctac aacgcgtagc gcgttacacc atgatcttgc tgatgctaac
     6361 agaattcgga gcgtggatat ggttacgtta gaacagttcc gttattgccc gacacactca
     6421 acgcatcctc cgttctgtta tgactttcac tatgtcaaac cggggatggt cgccatcttt
     6481 ggtgacaacg gcagtggcaa aagtacgctg gcacagctga tggcgggctg gtatccggac
     6541 tttctgccag gagagataac aggcacgggg acgctcctcg gtacgcccat tgggcatctc
     6601 ccgcttaatg aacaatccgc caccatccag ctcgtccagc aatcgcccta tttgcagctc
     6661 tccggctgta cctttagcgt ggaagaagaa gttgcctttg ggccagaaaa tctgtgtctt
     6721 gctgaaacag agattatggc gcggattgat gccgccctga ctctgaccga gtgccagccg
     6781 ttgcgccatc gtcacccggc aacactctcc ggtggagaga cacaacgcgt ggtcatcgcc
     6841 tgcgccatcg cgatgcagcc aaagctctta attcttgatg aagcctttag tcgcctgacg
     6901 ccgcaggcca gcgaaatgct gttgcagcgc ttacaacact gggcgcttga acgtggttcg
     6961 ctgattattc tttttgaacg tcatcgcaca cctttcctga actactgcca gcaagcgtgg
     7021 caattgcaaa acggagcgct ccagccacta tgttaacgtt aaatcagata tcttatcgct
     7081 ggcctagcgc agcaacggat tgtctttgcg acatttctct gcaactcaaa caaggtgaat
     7141 ggttggcgct gaccggcgac aatggcgcgg gaaaatcgac gctgttacga gtaatggcag
     7201 gtcttcttac gcccacagcc ggtacggtta tgctgcaaca acaggcgatg aaaaacctga
     7261 aaaaccgcca gcgcgcggca aaggttggcg tactgtttca ggaagcggaa aaccaacttt
     7321 ttcacagcac cgtcgctgac gagatcgcat ttggtttgaa actgcaaaaa tgcccggcgg
     7381 atgaaatcac tcaacgtacc catgctgccc tgcaatgttg tcagttagca gacactgcca
     7441 actcgcaccc gttggatctg catagtgcgc agcgacggat ggtcgccgtc gcctgcctgg
     7501 aagcactctc tccgccacta ttactgctgg acgagccgag tcgggatttt gacgaaaatt
     7561 ggttgagcgt gtttgaaagc tggctggaga agtgccgcca gcgaggcacg agcgtggtag
     7621 caatcagtca tgacgccgca tttacccggc gtcatttctc tcgtgtggtg cgactggaag
     7681 atggtgtaat caggaacgtt aatccaccag acgatattca cccgtagcgg tcatcattaa
     7741 ggtcaaattt gcaggcagac tctcttccag cacccggcga acattcggcc cgtcggtacg
     7801 gtcataaaag gcttctgcat ctgccagcga tacccctccc gccagcttgc gccccaccag
     7861 acgctcccgc aacgcagtag ctggcgcgtt gataaagatc gagaaatcac aaaattgcgc
     7921 aagctggcac cacttctcgt cgtccagcaa caaccagttt ccttcgacga tgacgagtgg
     7981 tgcggtaacg tgcaacgcat cttcaacagg atcatgcttt tgtcgatcgt actgcggcca
     8041 cgtacaatcc ccttccacca cctggcgcag attttccgcc agtttcgcaa cgtcaaatgt
     8101 ctctggtgcg cctttgaagg ggcgcaattg atgcgcgtcc agccagctat tgtaatggtg
     8161 aaaaccatcc atcgggagcg tctgaatagc gggcagctcc gggtcttgtt gcacgagata
     8221 ttcccagaag gtggttaggg tagatttacc cgtccccggc ggagcgcaaa gaaaaaccac
     8281 cgtacgccgc tgcggattta ccgtctgtaa ggccgccagc atacgcagca acggtttatg
     8341 cacgcgttct atctcttcat catggtactg cgcttgcacg ttcagcccat tcaccgttaa
     8401 ttcaattttc acggcgttaa ttcctttaag gtggtcgtta ctgccagcgt cagcgccgtt
     8461 ttgaccattc cgctaaaacg cgcc
//
LOCUS       DAJTYZ010000156         8389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000156 DAJTYZ010000000
VERSION     DAJTYZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.156"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            339..860
                     /gene="fecI"
                     /locus_tag="OO596_003571"
     CDS             339..860
                     /gene="fecI"
                     /locus_tag="OO596_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR6719338.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            857..1810
                     /gene="fecR"
                     /locus_tag="OO596_003572"
     CDS             857..1810
                     /gene="fecR"
                     /locus_tag="OO596_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR6719339.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1897..4221
                     /gene="fecA"
                     /locus_tag="OO596_003573"
     CDS             1897..4221
                     /gene="fecA"
                     /locus_tag="OO596_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR6719340.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            4266..5168
                     /gene="fecB"
                     /locus_tag="OO596_003574"
     CDS             4266..5168
                     /gene="fecB"
                     /locus_tag="OO596_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR6719341.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5165..6163
                     /gene="fecC"
                     /locus_tag="OO596_003575"
     CDS             5165..6163
                     /gene="fecC"
                     /locus_tag="OO596_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR6719342.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLCLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6160..7116
                     /gene="fecD"
                     /locus_tag="OO596_003576"
     CDS             6160..7116
                     /gene="fecD"
                     /locus_tag="OO596_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR6719343.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPGILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7117..7884
                     /gene="fecE"
                     /locus_tag="OO596_003577"
     CDS             7117..7884
                     /gene="fecE"
                     /locus_tag="OO596_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR6719344.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1838 a         2399 c         2334 g         1818 t
ORIGIN      
        1 ccttcagtag cggagcagga aggacatctg atggaaatgg aagccacgca agcaccttaa
       61 aatcaccatc atacactaaa tcagtaagtt gacagcatta ccgaaatccc tatcctttag
      121 ggcagtgagg atgtcaataa tagcagacca catcatgagt cattctgacc gacacatgcc
      181 agaagcagag gctccatccc attttatacc taccttataa cacttagaaa aacaacatgt
      241 taaaaatgtc tattggaaac aattttattt ccaattgtaa tgataactat tctcatatta
      301 atatggctac gtgataatta acttttgatg cactccgcat gtctgaccgc gccactacca
      361 cagcttcctt aacattcgag tcgctttatg gcacacatca cggctggttg aaaagctggc
      421 tgacgcgcaa actccagtct gcttttgatg cagatgacat tgcccaggac acttttttgc
      481 gggtaatggt cagcgaaacg ctctcgacga tccgcgatcc tcgctccttc ctctgcacta
      541 tcgccaaacg cgtgatggtg gacctgtttc gccgaaacgc gctggaaaaa gcgtatctgg
      601 agatgctggc gcttatgccg gaggggggag cgccttcacc tgaggaacgc gaaagccaac
      661 tcgagaccct acaactcctc gacagcatgc tggacgggct aaacggcaaa acacgtgaag
      721 cgtttctgct ttcgcaactg gatggtctga catacagcga gattgcgcac aaactcggtg
      781 tttccatcag ctccgtgaaa aaatacgtgg cgaaagccgt cgagcactgc ctgctgttcc
      841 gtctggagta tgggttatga atcctttgtt aaccgattcc cgccgtcagg cgctgcgttc
      901 agcttcccac tggtatgccg tgctaagcgg cgagcgcgtc agcccacaac aggaagcgcg
      961 ctggcaacag tggtatgaac aggatcagga taaccagtgg gcctggcagc aggttgaaaa
     1021 cctgcgcaac cagcttggcg gtgtgcctgg cgacgttgcc agccgggcgt tgcacgatac
     1081 ccgcctcacc cgccgtcacg tgatgaaagg attactgctg ttgctcggcg ctggcggagg
     1141 ctggcagctc tggcagtcgg aaaccggcga aggtctgcgg gcagattacc gcaccgccaa
     1201 aggcacagtc agccgtcagc aactggaaga tggctccctg ctcacgctga atacccaaag
     1261 cgcggcggat atgcgttttg atgcgcatca gcgcaccgtc cggctctggt acggtgaaat
     1321 cgccattact accgcgaaag atgccctgca acgccccttc cgtgtcctga cccgtcaggg
     1381 ccagctcact gctttaggga cagaatttac cgtccgccag caggataatt tcacgcagct
     1441 tgacgtgcag cagcacgccg tggaagtgct tctcgccagt gcccccgcgc aaaaacgcat
     1501 cgtgaacgct ggtgaaagcc tgcagttcag cgcctctgag tttggcgcag tgaaaccgct
     1561 ggatgacgag agtacaagct ggacgaagga catcctgagc ttcagcgata aaccgctggg
     1621 tgaggtgata gccacgctaa cccgttaccg caacggcgtg ctgcgctgcg atcccgccgt
     1681 tgccgggctg cgcctgagcg ggacgttccc gctgaaaaat accgatgcga tcctgaacgt
     1741 tatcgcgcaa acgcttcccg ttaaaattca gtctattacg cggtactgga taaacatttc
     1801 accactgtaa ggaaaataat tcttatttcg attgtccttt ttacccttct cgttcgactc
     1861 atagctgaac acaacaaaaa tgatgatggg gaaggtatga cgccgttacg cgtttttcgt
     1921 aaaacaacac ctttggttaa cgccattcgc ctgagcctgc tgccgctggc cggtctctcg
     1981 ttttccgctt ttgctgcaca ggttaatatc gcaccgggat cgctcgataa agcgctcaat
     2041 cagtatgccg cacacagcgg atttaccctc tcggttgacg ccagcctgac gcgcggcaag
     2101 cagagcaacg gcctgcacgg cgattacgac gtcgagagcg gcctgcaaca actgctggac
     2161 ggcagcggac tgcaggtaaa accgctggga aataacagct ggacgctgga gcccgcgccc
     2221 gcaccaaaag aagatgccct gaccgtggtc ggcgactggc tgggtgatgc gcgtgaaaac
     2281 gacgtatttg aacatgctgg cgcgcgtgac gtgatccgcc gtgaggattt cgccaaaacc
     2341 ggcgcaacca ccatgcgtga ggtacttaac cgcatccctg gcgtcagcgc gccggaaaac
     2401 aacggcaccg gcagccacga cctggcgatg aactttggca tccggggcct gaacccgcgc
     2461 ctcgccagcc gctcgaccgt cctgatggac ggcatccccg tccccttcgc cccttacggt
     2521 cagccgcagc tttcactggc tcccgtttcg ctcggcaaca tgaatgccat tgacgtggta
     2581 cgcggtggtg gtgcggtgcg ttacggaccg cagagcgtgg gcggcgtggt gaactttgtt
     2641 acccgtgcca ttccgcagga ctttggtatc gaggcgggcg tggaaggtca gctcagccca
     2701 acctcttcac aaaacaaccc gaaagagacg cacaacctga tggtgggcgg cacagcggac
     2761 aacggttttg gcaccgcgct gctctactcc ggcacgcgcg gcagtgactg gcgcgagcac
     2821 agcgccaccc gcatcgacga cctgatgctg aaaagcaaat atgcgccgga tgaggtgcac
     2881 accttcaaca gcctgctgca atattacgac ggtgaagccg acatgcccgg tggcctgtct
     2941 cgcgcggatt acgacgccga tcgctggcaa tccacccgcc cgtatgaccg cttctggggt
     3001 cgtcgcaagc tggcgagcct gggctaccag ttccagccag acagccagca taaattcaac
     3061 attcaggggt tctacaccca aaccctgcgc agcggctacc tggagcaagg caaacgcatc
     3121 accctctcgc cgcgtaacta ctgggtgcgc ggtattgagc cacgctacag ccagatcttt
     3181 atgatcggcc cttccgcgca cgaagtgggc gtgggctatc gctatgtgaa tgaatcaacg
     3241 catgaaatgc gttactacac cgccaccagc agcgggcagt tgccgtccgg ctcaagccct
     3301 tacgaccgcg atacgcgttc cggcaccgag gcgcacgcct ggtatctgga tgacaaaatc
     3361 gacatcggca actggaccat cacgccgggt atgcgtttcg aacatatcga gtcataccag
     3421 aacaacgcca tcacaggcac gcacgaagaa gtgagctata acgcaccgct tccggcgttg
     3481 aacgtgctct atcacctgac tgacagctgg aatctttatg caaacactga aggctcgttc
     3541 ggcaccgtac agtacagcca gattggcaag gctgtgcaaa gcggcaatgt ggaaccggaa
     3601 aaagcgcgaa cctgggaact cggtacccgc tacgacgacg gcgcgctgac ggcggaaatg
     3661 gggctgttcc tgattaactt taacaatcag tacgactcca accagaccaa cgacaccgtc
     3721 actgcacgtg gcaaaacgcg ccataccggg ctggaaacgc aggcacgtta cgatctgggt
     3781 acgctaacgc caacgcttga taacgtttcc atctacgcca gctatgcgta tgtgaacgcg
     3841 gaaatccgcg agaaaggcga cacctacggc aatctggtac cattctcccc gaaacataaa
     3901 ggcacgctgg gcgtggacta caagccagga aactggatgt tcaatctgaa cagcgatttc
     3961 cagtccagcc agtttgcgga taacgccaat acggtgaaag agagcgccga cggcagtacc
     4021 ggccgcattc ccggcttcat gctctggggc gcacgcgtgg tgtatgactt tggcccgcag
     4081 atggcagatc tgaacctggc attcggtgtg aaaaacatct tcgaccagga ctacttcatc
     4141 cgctcttatg acgacaacaa caaaggcatc tatgcaggcc agccgcgcac gctgtatatg
     4201 cagggggcgt tgaagttctg atgtgttaac gcccggcttg ccgggctttt agctggaatg
     4261 tgattatgtt ggcatttatc cgttttcttt ttgcaggcct gctgctggtg atcagccacg
     4321 cctttgccgc cacggttcag gacgaacacg gcacgtttac actcgaaaaa acgccacaac
     4381 ggattgtggt gctggaactc tcgttcgccg atgcgctggc cgccgtggac gtcagcccga
     4441 tcggtattgc cgacgataac gatgcaaaac gcatcctgcc cgaagtgcgt gcgcacctga
     4501 aaccgtggca gtccgtcgga acgcgcgcgc agccgagcct ggaagccatt gccgctctga
     4561 aaccagacct gatcattgcc gacagcagtc gccatgcggg ggtttacatc gccttgcagc
     4621 aaatcgcgtc ggtactgctg cttaagtccc gcaacgaaac ctacgctgaa aatttgcaat
     4681 ctgcggctat catcggcgaa atggtgggta aaaagcgaga gatgcaggca cgtctggaac
     4741 aacataaaga gaggatggcg cagtgggcca gccagcttcc caaagggaca cgcgtggcct
     4801 ttggcacatc acgcgaacag caattcaacc tgcatactca ggagacctgg accggcagcg
     4861 tgctggcctc tctggggctg aacgttcccg ctgcgatggc gggtgcgtcc atgccgtcca
     4921 tcggcctgga gcaactgctg gcggtcaatc ctgcctggct gctggttgcc cactatcgcg
     4981 aagagagcat tgttaaacgc tggcaacaag atccgctctg gcagatgtta accgccgcgc
     5041 agaagcagca ggttgcttcg gtcgacagta acacctgggc gcggatgcgc ggtatttttg
     5101 ctgcagagcg tattgccgct gacacggtaa aaatcttcca ccatcagccg cttaccgttg
     5161 tgaaatgacc gcgataaaac acccggtgct gctgtggggg cttcccgttg cagcacttat
     5221 tattattttc tggctgagtc tgttttgcta ctcggccatt cctgtttccg gagcagatgc
     5281 aacccgcgcc ctactgcctg gacacacgcc aacgctacca gaagcgctgg tgcaaaacct
     5341 tcgtttgcca cgaagcctgg tcgccgttct gatcggcgca agcctggcgc tcgcgggcac
     5401 gctgctgcaa accctgaccc acaacccaat ggcctctcct tcactgctcg gcattaacag
     5461 cggcgcggcg ctggctatgg cgcttaccag cgcgctgagt ccgacgccga ttgcaggcta
     5521 ttctctgtcg ttcatcgcgg catgcggggg cggcgtgagc tggctgctgg tcatgaccgc
     5581 aggaggcgga tttcgtcata cccatgacag aaacaaactg atcctcgcgg gtatcgcgct
     5641 gtcggccttt tgtatgggcc ttacccgcat caccctgctg ctggccgaag atcatgctta
     5701 cggcatcttt tactggctgg ctggcggagt gtcccacgcc cgctggcagg atgtctggca
     5761 gctcttgccg gtggtggtca ctgcagtccc tgtcgtgttg ctgctggcga atcaactgaa
     5821 cctgctcaac ctcagcgaca gcaccgccca tacgctggga gtgaacctga cgaggctatg
     5881 tttggtcatc aatatgttag tgctgcttct ggttggtgcg tgcgtcagcg tggcaggtcc
     5941 ggtggcgttt atcggtctgc tggtgccaca tctggcgcgc ttctgggcag gcttcgatca
     6001 gcgcaacgta ctgccagtga gcatgctgct gggggccacg ctgatgctgc tggcagatgt
     6061 actcgcacgc gcgctggcct tccccggaga tctgcccgca ggcgcagtgc tggcgctgat
     6121 tggcagccct tgctttgtct ggcttgtgag gaggcgagga tgaaaattgc gctggttatt
     6181 ttcatcaccc ttgccctggc gggctgtgcg ctgttatcac tccatatggg agtgatcccc
     6241 gtgccgtggc gcgcgctgct gaccgactgg caggccggac gcgagcatta ttatgtattg
     6301 atggagtacc gactgccgcg cttgctgctg gcactgtttg tcggtgcagc cctcgccgtg
     6361 gcgggcgtgc tgatacaggg gattgtgcgc aaccctctgg catcaccggg tattctcggc
     6421 gttaaccatg ccgccagcct ggcctctgtg ggggctctac ttcttatgcc gtcactgccc
     6481 gtgatggtgc tgccgctgct ggcctttgcg ggcggcatgg cggggttgat attgctgaag
     6541 atgctggcaa agacccacca gccgatgaag ctggcactca ccggcgtggc gctttctgca
     6601 tgctgggcca gcctgacgga ttatctgatg ctctcgcgcc cgcaggatgt gaacaacgcc
     6661 ctgctgtggc tgaccggcag cttatggggc cgtgactgga gctttgtgaa gattgccatc
     6721 ccgctgatga ttttatttct gccgctgagc ctgagttttt gccgcgatct cgacctcctt
     6781 gcactcggcg atgcgcgcgc caccacgctc ggtgtgtcgg tgccctatac ccgattctgg
     6841 gctttgttac tagctgtcgc catgacatct accggcgtgg ccgcctgcgg cccgattagc
     6901 tttattggtc tcgtggtgcc gcatatgatg cgtagcatca ccggtggacg tcaccgcaga
     6961 ctgctgcctg tttcggccct gacaggtgcg ttgctgttgg tggttgccga tctgctggcg
     7021 agaattattc atcccccact ggagctcccg gttggcgtgc tgaccgccat tatcggtgcg
     7081 ccgtggtttg tctggttgct tgtgagaatg cgataaatga ctttacgaac tgaaaatctg
     7141 acggtcaatt acggaacaga caaggtactt aacgacgttt cactctcact gccaacgggg
     7201 aagatcaccg ccctgatcgg tcctaacggt tgcgggaaat cgacgctgtt aaactgtttt
     7261 tcgcggcttt taatgccgca gtctggcacc gtatttctcg gcgataatcc cataaatatg
     7321 ctctcatcgc gccagttggc ccgcaggctt tcgctgctgc ctcagcacca tttaacgcca
     7381 gaggggatca cagtccagga gctggtttcg tatggtcgta atccctggct gtcactctgg
     7441 gggcgtctct ccgctgaaga caatgcacga gttaatgtcg ccatgaacca gacccggatc
     7501 aatcatcttg ccgttcgtcg gttaaccgag ctttccggcg gtcagcgcca gcgcgcattt
     7561 ctggcaatgg tcctggccca gaatacgccc gttgtattac ttgatgagcc aaccacctat
     7621 cttgatatca atcaccaggt ggacctgatg cggttgatgg gcgaactccg gactcagggg
     7681 aaaacggtgg tcgctgtgct gcacgacctt aatcaggcta gccggtactg cgatcaactg
     7741 gtggtaatgg caaacggaca tgttatggcg caaggcacac cagaagaggt gatgacccca
     7801 ggattgctga gaacagtatt cagcgtggaa gcggaaatac accccgagcc ggtatctggc
     7861 aggccgatgt gcctaatgag gtagattgca caggccgtaa gaaccaaacc acgactgaat
     7921 gaaactggac tggcgccagc aagcctgttc agactggggc tgaacttttc cggactctga
     7981 aagattacca atactcatcg tccatccgct tgctttaggc tgacaggttc ataatcaacg
     8041 caaaccagag ctgtacaggc ttgggcgcgg ctttcaaacc agtcgtgatc acggcaatca
     8101 attttgaact ctgcttaacg gacatttctg tataaccctt acggcaacga aaaacgcgaa
     8161 gttaaaattt tagaaaccca aaaacgtgac atgactaagt ttagatttca ggggggggag
     8221 atcaaaaaat ttcgctctgt gccagagcgg acattcacgg agctggttca ttaccaatga
     8281 ggttgggctt ttgaggataa atcaatgatc agacgccaac gtaaatcaaa agcaaccctg
     8341 gaacggaatt gctaatccag tttctgacca tcgatttttc taaaaagtg
//
LOCUS       DAJTYZ010000157         8272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000157 DAJTYZ010000000
VERSION     DAJTYZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.157"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            52..936
                     /locus_tag="OO596_003578"
     CDS             52..936
                     /locus_tag="OO596_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6719345.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO596_003579"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO596_003579"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR6719346.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO596_003580"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO596_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6719347.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="OO596_003581"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="OO596_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6719348.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="OO596_003582"
     CDS             5160..6443
                     /locus_tag="OO596_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR6719349.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="OO596_003583"
     CDS             6590..8065
                     /locus_tag="OO596_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6719350.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2192 a         1980 c         1914 g         2186 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacgtta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgc tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgacctgg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcagaaac tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgagacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgcccc gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actaaccgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc gg
//
LOCUS       DAJTYZ010000158         8215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000158 DAJTYZ010000000
VERSION     DAJTYZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.158"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OO596_003584"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OO596_003584"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCR6719351.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OO596_003585"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OO596_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR6719352.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2028..2546
                     /locus_tag="OO596_003586"
     CDS             2028..2546
                     /locus_tag="OO596_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6719353.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OO596_003587"
     CDS             complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OO596_003587"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6719354.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4773..5519
                     /gene="ydfG"
                     /locus_tag="OO596_003588"
     CDS             4773..5519
                     /gene="ydfG"
                     /locus_tag="OO596_003588"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6719355.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5608..6294
                     /gene="ydfH"
                     /locus_tag="OO596_003589"
     CDS             5608..6294
                     /gene="ydfH"
                     /locus_tag="OO596_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6719356.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6471..6674
                     /gene="ydfZ"
                     /locus_tag="OO596_003590"
     CDS             6471..6674
                     /gene="ydfZ"
                     /locus_tag="OO596_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6719357.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6710..8170)
                     /locus_tag="OO596_003591"
     CDS             complement(6710..8170)
                     /locus_tag="OO596_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6719358.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2171 a         2055 c         1870 g         2119 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata
     1981 cagaaaaagc aaatcacctt tacatataat agcgtggaca aaaaacagtg aacattaata
     2041 aagataaaat tgtacaactc gcagataccg acactattga aaacctgaca tccgcgttga
     2101 gtcaaagact tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta
     2161 agttggctag cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg
     2221 ttactttcac cgatcaggca aagatgaaaa tcctcagcgt aagccagcag tctcttgaac
     2281 gatattctgc ggtgagtgag aaagtggcag cagaaatggc aacaggtgcc atagagcgtg
     2341 cggatgctga tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg
     2401 gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa aggccagaac tacactgcgg
     2461 ttatgcattt tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg
     2521 cccagctgct gcaattactg ctataaccag gctggcctgg cgatatcccc aggccagcca
     2581 ttggtggtgt ttatatgttc aagccacgat gttgcagcat cggcataatc tgaggagcct
     2641 taccgcgcca ttgtcgatgc aggcgttcca gatcttcgct gttacctctg gaaaggatcg
     2701 cctcgcgaaa acgctgccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt
     2761 aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgca gcatatccgc
     2821 caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca
     2881 tattttccgc caccagcgcc cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt
     2941 cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat
     3001 accctttgtt gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg
     3061 ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt
     3121 cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg
     3181 cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta
     3241 ttacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga
     3301 cgttataaat taccggatgc gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc
     3361 atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca
     3421 cgccattatg atcaaaaatt tcccacaccc gaacgtcagg atggtagaca ggaatatcaa
     3481 aacgttcgac aaacttaata ccgaagagct gattcgcggt ccagaataca ccttcattca
     3541 acaccgtgtt taattcaaaa tatggcttga gctgcgcttc atcaagatca aatttctccc
     3601 gccgtacctg ttccgcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct
     3661 gcttatcgat aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgctg
     3721 gaacaatttc ccgcataaag ttgagtgctg cttctggcgt ttttgccatc tgatcggcga
     3781 ttttccatgc ggcataatga ggaaaaccaa gcagtttcgc ctgctgtgcg cgaatctcta
     3841 ccagacgttg aatgatagcg cgggtatcat tgccatcatt tttttccgct cgcgtccagc
     3901 ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt
     3961 gggtggtatt cagcagcgga atcagccatt tgttatccag acctttctcg cgagccgcct
     4021 ctgccgccag cgcaatctct tgctcactca ttcctgccag ctgcgcgata tcgttcacaa
     4081 ccagaccgcc ggatttattt gctgccagta atcgctggtt aaactggctg gtcagggtcg
     4141 cagcttctgt attcagtact tttaattttg ctttatcagc ttgcgcaagt ttggctccgg
     4201 caaggacaaa acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca
     4261 gggattcacg gcgctgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga
     4321 tatcattagc cagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat
     4381 cattggtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc
     4441 cgctttgttc cagtgccaga atagtattgt tgaaatcagg cgtttgcggg ttaagcgcga
     4501 tggcagcaat ttctgcccgc ttttgctgca ttccctcatc gaatgccggg cgatagtgat
     4561 gattggcaat ttgatcaaaa tggggagcca gatacggtag tgtgctttgc acaaggaaag
     4621 gattcattgt tgtcattttc ttctcctgaa cgcgagatgt tccatagcgt aggcttactg
     4681 ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt
     4741 attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg
     4801 caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca
     4861 ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga actgggagat aatctgtata
     4921 tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg
     4981 ccgagtggtg caatattgat atcctggtaa ataatgccgg cctggcgttg ggcatggagc
     5041 ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac aacaaaggcc
     5101 tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta
     5161 ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga
     5221 cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg
     5281 tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct
     5341 ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag
     5401 aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata
     5461 ccctggaaat gatgccggtt acccaaagct atgccggact gaatgtccac cgtcagtaat
     5521 ttttataccc ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc
     5581 agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag
     5641 cctgtcaatc agcaaattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca
     5701 ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg
     5761 gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt
     5821 ggcagctacg tcaacaaaat ttccatggcc caggtgcgca atggcagttt tatccgtcag
     5881 gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac
     5941 caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat
     6001 ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg
     6061 gcgtgggata ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc
     6121 gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgatatttt ctctgccctg
     6181 caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc
     6241 gaatccgtgc gccagatccg ccaggaaaat agcgactggt ttagcgaaga gtaattcatt
     6301 tcctctcatc ccatccgggg tgagagtctt ttcccccgcc ttatggctca tgcatgtatc
     6361 aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat
     6421 tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaatacct atgacgacct
     6481 acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc
     6541 acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg
     6601 gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc
     6661 tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg
     6721 aatatttcgc cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca
     6781 cttttgcagt aaacaaacta ttgtctggca aatcatcacc aaagatcgcc ttaatcgcca
     6841 gcaatgactg gacgcgcgct ttcccttcag cactactttg tacagccttc tgaataacag
     6901 gtaacagtgg gtcactgatt tcaatcggat ttccctgttc atcaacacca ccgacataac
     6961 gcatccaacc cgcgacgccc agtgccagca gatcgaactt gctgtcatgc gccagatgcc
     7021 agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc
     7081 aggttcgatg acgtaacgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt
     7141 gcaaatcaac gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg
     7201 ccgcataacg ataatgttca tcttccatgc agtcattaat gtgctgatat ccagcaagat
     7261 aacccaaata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat
     7321 aaggcagcac atcactaacc agttcggctc ccgctttttc ccattctgga cgtccggcaa
     7381 caaagttatc ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc
     7441 ccacaccggt aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac
     7501 ggtccaccat tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat
     7561 caacagcttt cgcgtatgaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt
     7621 tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcgg gccagcgcct
     7681 caacaatcac ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca
     7741 tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga
     7801 ttgtcagaga gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc
     7861 catccatctg tacgtgcaag gctttcttaa cgacgccaac gacgcgagcc gtccacgcat
     7921 cggccgacat ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct
     7981 gttcgccgcc gattaagttg acctcataat atccccagtc actgaaatgt tctgtagcaa
     8041 gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaataa
     8101 ttcttggggc caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat
     8161 tatttcccat aacaattcct taaatataaa tatggcaaac tatatgtttt gttat
//
LOCUS       DAJTYZ010000159         8155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000159 DAJTYZ010000000
VERSION     DAJTYZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.159"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..449)
                     /locus_tag="OO596_003592"
     CDS             complement(<1..449)
                     /locus_tag="OO596_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6719359.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="OO596_003593"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="OO596_003593"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6719360.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="OO596_003594"
     CDS             complement(1788..2507)
                     /locus_tag="OO596_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6719361.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="OO596_003595"
     CDS             complement(2691..3017)
                     /locus_tag="OO596_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6719362.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="OO596_003596"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="OO596_003596"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6719363.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="OO596_003597"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="OO596_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6719364.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="OO596_003598"
     CDS             4977..5252
                     /locus_tag="OO596_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6719365.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="OO596_003599"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="OO596_003599"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6719366.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /gene="nupG"
                     /locus_tag="OO596_003600"
     CDS             6598..7854
                     /gene="nupG"
                     /locus_tag="OO596_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6719367.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8155)
                     /locus_tag="OO596_003601"
     CDS             complement(7903..>8155)
                     /locus_tag="OO596_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6719368.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1921 a         2160 c         1987 g         2087 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaattc
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc cgtatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggtcaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcga
//
LOCUS       DAJTYZ010000160         8095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000160 DAJTYZ010000000
VERSION     DAJTYZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.160"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(102..797)
                     /locus_tag="OO596_003602"
     CDS             complement(102..797)
                     /locus_tag="OO596_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6719369.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(790..2217)
                     /locus_tag="OO596_003603"
     CDS             complement(790..2217)
                     /locus_tag="OO596_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6719370.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2228..2947)
                     /locus_tag="OO596_003604"
     CDS             complement(2228..2947)
                     /locus_tag="OO596_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6719371.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3475..4329)
                     /gene="rclR"
                     /locus_tag="OO596_003605"
     CDS             complement(3475..4329)
                     /gene="rclR"
                     /locus_tag="OO596_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCR6719372.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4555..5880
                     /locus_tag="OO596_003606"
     CDS             4555..5880
                     /locus_tag="OO596_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCR6719373.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5989..6225
                     /locus_tag="OO596_003607"
     CDS             5989..6225
                     /locus_tag="OO596_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6719374.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6237..6830
                     /gene="rclC"
                     /locus_tag="OO596_003608"
     CDS             6237..6830
                     /gene="rclC"
                     /locus_tag="OO596_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCR6719375.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6990..7859)
                     /locus_tag="OO596_003609"
     CDS             complement(6990..7859)
                     /locus_tag="OO596_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6719376.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2189 a         1983 c         1728 g         2195 t
ORIGIN      
        1 cagcataacc atgacggaac ggggttaagt caggttgtcc ttaacgctat tttcaacctc
       61 gtatgccttc ttcaggttta tgttcagatc tcatatctct ctcagcaatc ctcaataatc
      121 agatacaccg ctttcaccgg accatgaacc ccgacgactt tgataagctc aatatccgcc
      181 gttgagctgg gaccactgat gatgttaata catgacggca ttcgttcacc ggcctgcgct
      241 ttctgatgca atttttctgc gagttgcgct acacgcggca ggattgtgct tttacgcagg
      301 ataaaaagag aagattccgg gagcaggctc aatgaacgcc cgcgctcgac ggcggaaaaa
      361 agaaccacgc ctcccgattc ggttaaacca tattcagcat acacgacacc cactttagcc
      421 tgctctgcct gcgagatatt ctcggcacct ttcgccggat cccaaacaac ggcattgcat
      481 tcctgctgca aacgttcact aatccccaat tcctccagcc tcgtgtcacc gctaatcacg
      541 accgactgat ctcccagctc tttacacaga cgtattgcag cttctgccgc cttcgcctcg
      601 ctggtcagct cacagcgcgt caacataaca tcgctggcaa actgaataaa cgcgtcacag
      661 cgctgctgtt ggttaagttg ggtaagccgc tcgttagcat agttgttaag cggcgcatct
      721 tctgcttgcg gttcaagtcg cagcgggcga cccagtgcct gagcaacgtt attcaaaaat
      781 tcgctccgat tatccattct ttttctcctg cgcctgatgt ttcttaaacc aactacggaa
      841 actctctccg tcagcttcag gaagatcgcg tgcttccatc cagtcgctaa tcgcgccaaa
      901 tttgagtggt gttttgccgc cattgataaa ccagcttgcc gcatgagcgc cggccatcat
      961 cccgactttc cacaatcctg gatgactatt ggcataagcg aacattttta tcgcccgttg
     1021 ctctgctttt gcggtgatcc ctttttcagc catcacccga cgatgacgca aaatcagttt
     1081 tgacagcgga atacgcaccg gacacacgct gtcacaagca gtgcataaag agcaggcgta
     1141 gggtaaatct ttaaaatctt tatagccgcc aagtagcgga gaaatcaccg caccaattgg
     1201 ccctggataa atagagccat atccatgacc gccaatatgg cgatatgccg gacaagtatt
     1261 catacaagcc ccgcagcgaa tacagcgcag cacatcccga aattcagagg ccagcacctc
     1321 agaacgcccg ttatcgacaa taaccagatg aaactcttca ggaccatcaa cgtgccccgc
     1381 ttcgcgcggt cctgtcagcc aggtgttgta tcccgtcaaa cgtgcaccaa cggcactgcg
     1441 cgccagcatg gtgatcaata catctacctc ggcaaacgtg ggggcaatac gctccattcc
     1501 catcacagca atatgcgttt taggcagcgt ggtacacatt cgcgcattac cttcattggt
     1561 caccaggcat accgaacctg tctctgccac cgcgaaatta cagccggtaa tacctatttc
     1621 agcactgagg aaatcttcgc ggattttttg ccggatgaat aaggtcatcg cttcaggcgt
     1681 ttccggcccc tcatagccca gacgttcgtg tagcactcga cggatctgat ggcgatcttt
     1741 atgaattgcc gggaccacaa catgagatgg cggatcttga tccagctgga gaatatattc
     1801 acccagatcg gtttcaatca cctgaatgcc agcatcctgc aacacatgat tgacaccaat
     1861 ctcttcggtc accatcgatt tagatttcac caccttccgg gcatttttgc gttgggcaac
     1921 ctgtaaaatg tagcgggtag cgtcttcttt ggtttttgca aaccagacgt gaccgccgtt
     1981 ttgcgtcact ttttctgaga gctggtacag ataagcgtcg agattactca gaacatgatc
     2041 acgtatctgg gcggcccgat cgcgccactc ctcccagtgc cccaattcat cgaccatttt
     2101 ttgccgattt gccccaatac gctgctgcgc gtttgccacc gctttgcgca tgatcggatc
     2161 ttcaatttgc tgacggatgc gtgtcttaaa atctgtatta ctggttttga tcgacatctt
     2221 tatatcctca gcggctcatc aacacttcag caatatgcat cactttgact ttctgccctt
     2281 cccgttgtaa tcgcccactg atgttcagca ggcaactcac gtcagcacca attaaatact
     2341 cagggcggac ttccatcagg tgcacaacct tttctttcac catctcgccg gatatttcgg
     2401 ccattttgac cgagaacgtg ccgccaaatc cgcagcaggt atcctgttca gcaaaggtca
     2461 acagctccag tccacgcaca tttttcagca gcgtaagtgg ctcgtccttc actcccagct
     2521 tacgggccag gctacaagat gggtgataca cagctctccc ttgcaaactg gcacctacat
     2581 cgactacccc taatttatta acaataaaag aggtgagatc ctgcatacgc gcggcaacct
     2641 tttcggcacg tgatgcccat tcaggttcat ccgccagata cgtcgggtaa ctttttacgg
     2701 cataggtgca agagccagcc ggtgaaataa tgggatcgtc gttatcctcc agtgcggcga
     2761 tcagattttt catccctgga atcgcttctt tgatataacc gctattgatc gcaggctgac
     2821 cgcagcatcc ctgtttctcc gggaaattta cgcgacagcc gagtttttcc agtagcagca
     2881 cggagtctcg tgccattctt gatttcaggg cgtcaccaat acaggtgaca aagaaattga
     2941 cattcacaac taatactcca ttacttcatg cccatttatg cccgatggtt cccctccttc
     3001 cgggggatca atcatcaata aatatggcgt tcttttttaa ttggctagca acataccacc
     3061 cctcatttgt gtgacaaatg ataatctttc atccaaactt caaatgcaat aactaatttt
     3121 atttctttcg gcatcgctca cattttatgg ggaaatcgac gttcattttt ggcataagaa
     3181 atccatttaa aaaggctaca tcccttatac ctattcagag ctacttattc aggggttatt
     3241 gagatataca ctgatggtgt acaaaaaaat caccgacaat aatcacgtat caagtaatag
     3301 tcggggtttt atttaattga agcaatttag tttgcatttt tatttaaaaa ctgagctaga
     3361 gtccatcaga aaaaaatata taaagcccaa accaccgttg gaaatagaaa tagttgaaag
     3421 gctcacaact cccggaatgc ggattatctt tgccctgatt tctggcggtt ttattcaggg
     3481 tgcaagctgt ctgacccttt cccgatattc tcccggagta caaccaaact cgcggacaaa
     3541 cgccttgtga aaagatgatt cactggcata gcctactgac tcagcgatca ccacaacagg
     3601 gagcatttcc cgggaaaaca tctgggccgc tatttgtaga cgcaactttg ttaatacagc
     3661 cagcggcgtg gttccggaaa catcacggaa aagctgggca aaacttgccc gagacatgtg
     3721 ggcgatgctg gccagcgatt cgacggtcca ggcgtgtcct ggcatttcca gcatttgctg
     3781 tattaccgca ccaagacgtg gatgcagaag caaactgaga atgtttttct ctgtattaac
     3841 ctgtgcaatc cactcacgca ccgcgagggt aaagaatgta gcgcagatct ggctacacag
     3901 tgcatccact cccggcatcg ccgatctgga ttcctgttgt aaaaacggga tcgcctccct
     3961 cagccagtta tattccacgc tgtggttaac cggtgctaaa aacagcgttt ccggcaaaga
     4021 cgttaaaaaa taacgcgccg aatgttgcaa ccgaagagtg ccgcagacaa tacaggtcga
     4081 ttcattatcg acatgactca ggcgatgagc tgaattttgt ggtagcagga ccacatttcc
     4141 cgggcgtaat gtaaaaatct ctcccgtcgg catttccagc ttcgccgctc cttgcgttaa
     4201 cgcatgccaa cgaataaccg ataattcccc ggcaccatgt tgaagctgcc agtcacttcc
     4261 taacacgcaa ttcttatcga tcgttccttg tggagcgtta agcatcaaca aacggctaag
     4321 ggcatccata tcagactcct gagtggtttt tgattacaaa caccaacaat cttagtaaat
     4381 aactaatcta ataccatcaa atggttaatc atgatttttg ttttgtgatt ctgattaaga
     4441 aaaagaaaat accagacgat aagacaaaat atcaagcgtt gtgaagaaat ggtatttgct
     4501 cttttgcgtc tatagtcatg atgtcaaatg aacgtgtttc gacaggaaat catcatgaat
     4561 aaatatcagg cagttattat tggttttggc aaggctggaa aaacattagc cgtcacgctg
     4621 gcaaaagcag gttggcgagt ggctctcatc gaacaatcaa atgcaatgta tggcgggacc
     4681 tgtattaata tcggctgcat cccaaccaaa acattggttc atgacgcaca gcagcacaca
     4741 gattttgtcc gtgccataca gcgtaaaaat gaagtggtta attttttacg taataagaat
     4801 tttcataatc ttgcggatat gcccaatatc gacgtgatcg acggccaggc ggagtttatc
     4861 aataatcata gcctgcgtgt tcatcggcct gagggaaatc tggaaattca tggcgagaaa
     4921 atttttatta ataccggtgc acaaaccgtg gttccgccaa ttcctggaat taccaccacg
     4981 cctggtgtat atgacagcac cggattactt aatctaaaag aattgcctgg gcatttaggt
     5041 attttgggcg gcggatatat tggcgttgag ttcgcctcta tgttcgctaa ttttggcagc
     5101 aaggtaacca ttttagaagc agcttcgctg tttttgcctc gggaagatcg ggatattgct
     5161 gataatatcg cgacgatttt acgcgatcag ggcgtcgata ttatcctcaa tgcccatgtg
     5221 gagcgaatca gtcaccatga aaatcaagtg caagtgcata gcgagcacgc ccaactggcg
     5281 gtggatgcac tgttaatagc ttccggtcgt caaccggcta ccgcttcgtt acatccagaa
     5341 aatgccggta tcgcagtaaa cgagcgcggg gcaattgtcg ttgacaagca attacatacc
     5401 accgcagaca atatttgggc gatgggagat gttaccggcg ggctgcaatt tacttatatt
     5461 tcactggatg attaccgcat tgtgcgtgat gagttgctgg gagaaggcag acgtagtact
     5521 gatgatcgga aaaatgtgcc ttattccgta ttcatgacac cgccactgtc cagggttggt
     5581 atgacagaag aacaggccag agagagtggt gctgatattc aggtggtgac attgcctgta
     5641 gctgcaattc cgcgtgccag agtgatgaat gatactcgtg gggtattaaa agcgattgtt
     5701 gataataaaa cccaacgtat attaggggca tcactgctgt gtgttgactc ccacgagatg
     5761 atcaatatag tgaaaatggt gatggatgcc gggctgcctt atagcatatt acgcgatcag
     5821 atatttactc atccgtcgat gagcgaatca ctcaatgatc tattttcatt agtcaaataa
     5881 actcaaaatc agacgccaga acaaatattc tggcgtctca gagaaaagaa tcttattaat
     5941 tctctgttac tctataactt cttaatcact tcattgatgg tattttatat gtttaaaaaa
     6001 tctgttttat ttgcaacact attatctggc gttatggaat tttccaccaa tgcagatgat
     6061 aaaataattc tgaaacatat cagcgtctcg tcagtatcag catcaccgac agttctggag
     6121 gataccattg ctgatatagc cagaaaatat aatgcttcat cctggaaagt cacatcgatg
     6181 cgaattgata ataattcaac cgcaacagca gtattgtata aataaggatg ttcacaatgg
     6241 aaaaatacct gcacctgtta agtcggggag ataaaattgg cctgtcattg attcgtctga
     6301 gtattgcgat tgtttttatg tggattgggt tattaaagtt tgtcccttac gaggcagaca
     6361 gcattacacc attcgtcgca aacagtccac taatgtcgtt cttttatgaa cacccggaag
     6421 actataaaca gtatctgact cacgaaggcg aatacaaacc agaagcaagg gcatggcaat
     6481 cggccaataa tacctatggt ttttccaacg gtcttggcgt cgtggaggtg attattgctc
     6541 tgctggtttt ggctaatcct gtcaatcgct ggttaggttt attgggagga ctgatggcat
     6601 ataccacgcc gctggtaaca ctctcatttt taatcaccac cccggaggca tgggtacccg
     6661 cattgggtga cgctcatcat ggtttccctt atttatccgg tgctggtcgc ttggtattga
     6721 aagatactct gatgctggca ggtgctgtca tgataatggc agattcggcg cgggaaattc
     6781 ttaaacaacg cagtaatgaa tccagttcaa cgttaaaaac tgaatattga taatccgaac
     6841 actgtctgtt gttcactttt tcaggcgtca ggcccccttt ctgatgatga agcctgacgc
     6901 catattaccg gagatatgac agagtgaaga tcaatatcac tcccctgtta aaagattctt
     6961 gtcgtctcag gtaaaatata tgattaatat caatcatgta tttttacagc aagacaacca
     7021 cgaacaaatt caggattaaa atgttttacc gactccccaa cataacccaa atcaagcgaa
     7081 ctaatttgtg ccatttcttt ctctgtcagt gagaaatccc agatagcaaa attttcttcg
     7141 atacgctgtt gatgtgtcga tttcggaata acgaccactc cccgttgaat attccaacgc
     7201 agaatgactt gagaaatcga tttttgatgg gcatctgcaa tactctgaag cagattattt
     7261 tcaaagggtt tatgtcgtcc accacctaac ggagcccatg cttcaggctg cacgttataa
     7321 tacttcatgg ttgccagcgc ctctggttgg gcaaaatagg gatgcaactc gacctggttg
     7381 accatcggtt taactcttac cgtttcacaa aagttcgcca gaacatgagg atagaaattg
     7441 gaaaccccaa ttgcttttaa tttgccctct tcataggcat cttcgagtgc acgccacaca
     7501 ctgaaataat cgcgcatagc ctggtgcaat aaatagaggt cgaagtactc tagtcccgat
     7561 ttttttaatg atgcttcaat acctgctgct gccgtatcct gattcaacat atcctgcccc
     7621 cacaatttag acgttataaa taactcatct cgggtacata aaccttcaga tatagcttca
     7681 cgaacggctt caccgacggc atcttcatta ccgtataccg ctgcagtgtc gatgagtcga
     7741 taacccgtgc ggatagcgtt caacactgac tgtttgcaca cgtttttatc ggtaacctga
     7801 aaaacaccaa atcccatcat cggcattttc agattgttac ttaatacgga aaattccact
     7861 gtcagtccct ccattaggca tgaacaatga gtctacgtta aaacataacc tcaaattagt
     7921 atgtggattt tgatatcact tatgcaaaaa attcattaat aatgtaggac tgaaacctct
     7981 ctattttcgg ggacaacgaa gcagacgcta ccagtgcttt tgccttcgac cttgctgttt
     8041 ttgatacact tagggccttg ggcataacga aaatgtgcga tatcacgaag aatta
//
LOCUS       DAJTYZ010000161         8058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000161 DAJTYZ010000000
VERSION     DAJTYZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.161"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            267..1925
                     /locus_tag="OO596_003610"
     CDS             267..1925
                     /locus_tag="OO596_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6719377.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="OO596_003611"
     CDS             complement(1922..2878)
                     /locus_tag="OO596_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6719378.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="OO596_003612"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="OO596_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6719379.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="OO596_003613"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="OO596_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6719380.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="OO596_003614"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="OO596_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6719381.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6789..8027
                     /locus_tag="OO596_003615"
     CDS             6789..8027
                     /locus_tag="OO596_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6719382.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRYELNRAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGALPLEKCELRHWLACF
                     DQMAKRSPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRATELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVDPHVVEQLTAHQLPGVQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDEKIVVMTPRIYSQNS"
BASE COUNT         1978 a         1909 c         2189 g         1982 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatttatc atctagtgtt cgcgggtgtt cgtggtagtg ttttgtgtgc
     6661 attcatcgct atgattataa aggaaaattt tgttttcctt gttcgctctt gttcgtcacg
     6721 gtttgccctt gtacgatgca cggtgtagtc aatggtgtag ttaattttga tgaccggaaa
     6781 cgcgaaacat gagcagcgca attcatagac ttagcgacac tcttttacga aaattaagcg
     6841 gatcaccaac cacaaaaaac gcctttttta atgacggtgg caatcttagc gtaagacatt
     6901 ccaccagcgg cctgttaacc tggtatttca cttacagggc cggaactggc aggcaggtat
     6961 caccggaacg cctgagactg ggcaactatc ccgatctgag cctgaaagca gccagggaaa
     7021 aagcggcaca gtgtcgcgcc tggctggctg aggggaaaaa tccgcgctat gagcttaatc
     7081 gtgctgtaca ggatgcgtta gcccccgtga cagttaagga agcgctcact tactggcttg
     7141 aatcgtatgc aaaggaaaag cgcactgact acgaatcact gaaaagccgg atcaataaac
     7201 acataatcag ccagattggc gctttgccgt tggaaaaatg cgagctacgg cactggttgg
     7261 catgttttga ccagatggca aagcgaagcc cagtatctgc cggatttttg ctacaggtat
     7321 gcaaacaggc gcttaagtac tgccgaaaac gacgatacgc aatcagcaac gttcttgatg
     7381 atatggttgt cggtgatgtg ggaaaaaaag cagaaataag cgagcgcgta ctaacaaaca
     7441 aggagcttgg ggaattactc cgcgccctgg atgaaaaaat ttacccaccg tactacagcg
     7501 ccctgattcg cctcctgatt gtcttcggat gccgtgccac tgagctaaga cgctctgagg
     7561 tccaggaatg ggattttaaa gaaatgctct ggaccgtgcc gaaagaacac agcaaaacga
     7621 aggtagcaat attcaggcca ataccggaag cgatcttgcc gttcgtaacg aagctggtgg
     7681 agcagaacag gcacacggga ttattgctcg gagaactgaa agggcaatca tccgtatcag
     7741 agtacggaag aacggcacac agacgcatta atcaagcccc ctggacgttg cacgacatcc
     7801 ggcacacgtt tacaacgatg ctgaacgatt taggcgtgga tcctcatgta gtggagcagc
     7861 taaccgcgca ccagttgcca ggagtgcaac gagtctataa tcactcccgt tatctcgatg
     7921 caaaacgtga cgctcttaat ctatgggttg agcgtctcga gcttctccag aacaatgatg
     7981 aaaaaatcgt tgttatgacc ccgcgaattt actcccaaaa ttcttgacaa attacggctg
     8041 tttttcttgc gaaaaatg
//
LOCUS       DAJTYZ010000162         8032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000162 DAJTYZ010000000
VERSION     DAJTYZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.162"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(266..1297)
                     /gene="rsmC"
                     /locus_tag="OO596_003616"
     CDS             complement(266..1297)
                     /gene="rsmC"
                     /locus_tag="OO596_003616"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6719383.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1400..1813
                     /gene="holD"
                     /locus_tag="OO596_003617"
     CDS             1400..1813
                     /gene="holD"
                     /locus_tag="OO596_003617"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6719384.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1782..2228
                     /gene="rimI"
                     /locus_tag="OO596_003618"
     CDS             1782..2228
                     /gene="rimI"
                     /locus_tag="OO596_003618"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6719385.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2243..2920
                     /gene="yjjG"
                     /locus_tag="OO596_003619"
     CDS             2243..2920
                     /gene="yjjG"
                     /locus_tag="OO596_003619"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6719386.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3011..4600
                     /gene="prfC"
                     /locus_tag="OO596_003620"
     CDS             3011..4600
                     /gene="prfC"
                     /locus_tag="OO596_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6719387.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4993..5598
                     /gene="osmY"
                     /locus_tag="OO596_003621"
     CDS             4993..5598
                     /gene="osmY"
                     /locus_tag="OO596_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6719388.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5725..5886
                     /locus_tag="OO596_003622"
     CDS             5725..5886
                     /locus_tag="OO596_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6719389.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6008..7081
                     /locus_tag="OO596_003623"
     CDS             6008..7081
                     /locus_tag="OO596_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6719390.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7078..7860
                     /locus_tag="OO596_003624"
     CDS             7078..7860
                     /locus_tag="OO596_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6719391.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1914 a         2074 c         2181 g         1863 t
ORIGIN      
        1 accatcaatt cttaaaaaga attgctacca cggaggcgca ttctagtggt tttcagcttt
       61 tcgtcaatag ttaattatcg ccggaggtgt aattgctgga aaaatgtcca tcaggaaact
      121 agcgtgcagg tttggtaggc atgcgggggc agatgccaga tgcgacgctg gcgcgtctta
      181 tctggcctac gaagggctaa cgtgcaggtt ttgtaggtcg gataaggcgt tcacgccgca
      241 tccgacacgg tattcggcga gataattaac ctttcttcgc ctggcgggtc ataatggcgc
      301 gatacacctt gaaacgcccg gtttgcgcaa tcacttcatg gaagccaaat gtctcatcca
      361 gcacgtccgg gtaaggcagg aaggcgttcg ctacaattcg cagctcgccg ccgctattaa
      421 gatgacgcac cgcgccgcga atcagcgttt gcgccgcatc caggctggtt tgcatcccat
      481 cgtggaacgg cgggttggag atgatcatat caaaacgacc tttcacctcg gaaaagacgt
      541 tgctggcaaa gacttcacct tcaacacagt tggccgcaag tgttgcgcgg ctggcttcta
      601 ccgctggcgc agagacatcg cacaaggtga gacgaatttt cggcgaatgg cgcgcaaagg
      661 caactgaaag cacccccgcg ccacagccga catccagcac tttacctttc gtgtgcggag
      721 ttaacgtcga gagcagcaac tggctaccga catccagacc gtcgcggcta aacacgccag
      781 gcagcgtttt gaccgtcagg ccatcgacgc tgtattcgcc ccagaattta tcggcatcaa
      841 ataccggctg tttttccaga cgaccaaaat agaggccaca gcgacgagcg ctgtcgactt
      901 tattcaacgg cgcataatct gccagcatct gctcggcgct gcgcacgccg ctgcggttct
      961 cgccaacgac aaaaatatct gtccccactg gcagcagaga aagtaaattc atcaactgga
     1021 actgggcttc cggtttgttc ttcggccagt agtaaatcag catatcgcaa tctgcgacgt
     1081 catccaccgt ggcgaccaga ctgaaacggg cgttatcccc catctggcgg cttaatacct
     1141 gccagtggtg gaattgctgg gtatgagcac ggctggccgc ggtatctaaa cgcgcgggca
     1201 ggtcatcctg taagtctccg gcaaacagaa tacggctttg ttcgaaatca tcactgtgac
     1261 gcagcaagac ttcacttgcc ggggtaaatg cagacatgga atgctcctca attgatactg
     1321 gcggcgatta tagccatatg ttggcgcggt atcgacgaat ttgctatatt tgcgcccctg
     1381 acaacaggag cgattcgcta tgacatcccg acgagactgg cagttacagc aactgggcat
     1441 tacccagtgg tcgctgcgtc gccctggcgc gttgcagggg gagattgcca ttgcgatccc
     1501 ggcacacgtc cgtctggtga tggtggcaaa cgatcttccc gccctgaccg atcctttagt
     1561 gagcgatgtt ctgcgcgcat taaccgtcag ccccgatcaa gtgctgcaac tgacgccaga
     1621 aaaaatcgcg atgctgccgc aaggcagtcg ctgcaacagc tggcggttgg gtactgacga
     1681 accgctatca ctggaaggcg ctcaggtggc atcaccggcg ctcaccgaat tacgggcaaa
     1741 cccaacggca cgcgccgcgt tatggcaaca gatttgcaca tatgaacacg atttcttccc
     1801 tcgaaacgac tgatttacca gcggcttacc acattgaaca acgcgcccac gcctttccgt
     1861 ggagtgaaaa aacctttgcc agcaaccagg gcgagcgtta tctcaacttt cagttaacgc
     1921 aaaacggcaa aatggcggcg tttgcgatta cgcaagtggt gctggatgaa gctacattgt
     1981 tcaatattgc ggtcgatcct gactatcagc gtcagggatt gggaagggtg ctgctggaac
     2041 atctgatcga cgaactggaa aaacgcggcg tggcgacact atggctggaa gtccgtgcct
     2101 caaatgctgc cgccattgcc ctgtacgaaa gtttaggctt taacgaggcg acgattcgcc
     2161 gcaattacta ccccaccacg gacggtcgcg aagacgccat catcatggcg ttgccaatca
     2221 gtatgtaaga caaggtggaa taatgaagtg ggactggatt ttctttgatg ccgatgaaac
     2281 gctgtttacc tttgactcat tcaccggcct gcagcggatg tttcttgatt acagcgtcac
     2341 ctttaccgct gaagattttc aggactatca ggccgttaac aagccactgt gggtggatta
     2401 tcaaaacggc gcgatcactt cattacagct tcagcacggg cgttttgaga gctgggccga
     2461 acggctgaaa gttgaagcag gcttgcttaa cgatgccttt attaatgcga tggcggaaat
     2521 ctgcacgccg ctgccgggcg cggtttctct gcttaacgcc attcgtggca acgccaaaat
     2581 cggcatcatc accaacggct ttagcgcctt gcagcaagtg cgtctggaac gcacgggcct
     2641 gcgtgattac ttcgatttgc tggtgatttc cgaagaagtt ggcgttgcca aaccgaataa
     2701 gaaaattttc gattatgcgc tggaacaggc gggcaatcct gaccgttcac gcgtgctgat
     2761 ggttggcgac actgccgagt ccgatattct cggtggcatc aacgccgggc ttgcgacctg
     2821 ctggctgaat gcgcacaatc gcgagcaacc agaaggcatc gcgcccacct ggaccgtttc
     2881 ttcgttgcac gaactggagc agctcctgtg taaacactga ttgcctcccc cccgttgatg
     2941 ggtaaaatag ccgcaatttt tcgttttcaa caagcgcggc gcgatgccgc ttactcaaga
     3001 agaaagaatt atgacgttgt ctccttattt gcaagaggtg gcgaagcgcc gcacttttgc
     3061 cattatttct cacccggatg ccggtaagac taccatcacc gagaaggtgc tgctgttcgg
     3121 acaggccatt cagaccgccg gtacagtaaa aggccgtggt tccaaccagc acgctaagtc
     3181 ggactggatg gagatggaaa agcagcgtgg gatctccatt actacgtctg tgatgcagtt
     3241 tccgtatcac gattgcctgg ttaacctgct cgacaccccg gggcacgaag acttctcgga
     3301 agatacctat cgtaccctga cggcggtgga ctgctgcctg atggttatcg acgctgcaaa
     3361 aggtgttgaa gatcgtaccc gtaagctgat ggaagttacc cgtctgcgcg acacgccgat
     3421 cctcaccttt atgaacaaac ttgaccgtga tatccgcgac ccgatggaac tgctcgatga
     3481 agttgagaac gagctgaaaa tcggctgcgc accgatcacc tggccgattg gctgcggcaa
     3541 gctgtttaaa ggcgtttacc acctttataa agacgaaacc tatctctatc agagcggtaa
     3601 aggccacacg attcaggaag tccgtattgt taaagggctg aataacccgg atctcgacgc
     3661 tgcggttggt gaagatctgg cacagcagct acgtgacgaa ctggaactgg tgaaaggcgc
     3721 gtctaacgag ttcgacaaag aattgttcct tgcgggcgaa atcactccgg tattcttcgg
     3781 tactgcgctg ggtaacttcg gcgttgatca tatgctggat ggcctggtgg agtgggctcc
     3841 tgcgccgatg ccgcgtcaga ctgatacccg taccgtagag gcgagcgaag acaaatttac
     3901 cggcttcgta tttaaaattc aggccaacat ggacccgaaa caccgcgacc gcgtggcgtt
     3961 tatgcgcgta gtatccggta aatatgaaaa aggcatgaag ctgcgccagg tgcgtactgc
     4021 gaaagatgtg gttatctccg acgcgctgac ctttatggcg ggcgaccgtt cgcacgttga
     4081 agaagcgtat ccgggcgata tcctcggcct gcacaaccac ggcactattc agatcggcga
     4141 cacctttacc cagggtgaga tgatgaagtt caccggtatt ccgaacttcg caccagaact
     4201 gttccgtcgt atccgcctga aagatccgct gaagcaaaaa cagctgctca aagggctggt
     4261 acagctttcc gaagagggcg cggtgcaggt gttccgtccg atctccaaca acgatttgat
     4321 cgttggtgct gttggtgtgc tgcagtttga tgtggtggta tcgcgcctga agagcgaata
     4381 cagcgttgaa gcagtatatg aatcagtcaa cgttgccact gcccgctggg tagaatgtgc
     4441 ggacgcgaag aaattcgaag agttcaagcg taagaacgaa agccaactgg cgcttgatgg
     4501 cggcgataac ctcgcttaca tcgctaccag catggtcaac ctgcgcctgg cgcaggaacg
     4561 ttatcccgac gttcagttcc accagacccg cgagcattaa ttcttgcctc cagggcgcgg
     4621 tagccgctgc gccctgtcaa tttcccttcc ttattagccg cttacggaat gttcttaaaa
     4681 cattcacttt tgcttatgtt ttcgctgata tcccgagcgg tttcaaaatt gtgatctata
     4741 tttaacaaag taatgacatt tctgacggcg ttaaataccg ttcaatgcgt agatatcagt
     4801 atctaaagcc gtcgattgtc attctaccga tattaataac tgattcagag gctgtaatgg
     4861 tcgttattca tcacccatcg cttttgtgat ggcgaccatt gacttctgta gagggtgaag
     4921 tctctcccta ttcagcaatg caacctcgtg ttgccaggct caaattacga gcaaacatac
     4981 aggaataaat cgatgactat gacaagactg aagatttcga aaactctgct ggctgtaatg
     5041 ttgacctctg ccgtcgcgac cggctctgcc tacgcggaaa acaacgcaca gactaccaat
     5101 gaaagcgcag ggcaaaaagt cgatagctct atgaataaag tcggtaattt catggatgac
     5161 agcgccatca ccgcgaaagt gaaggcggcg ctggtggatc atgacaacat caagagcacc
     5221 gatatctctg tcaaaaccga tcaaaaagtc gtgaccctga gcggtttcgt tgaaagccag
     5281 gctcaggccg aagaggcagt gaaagtggcg aaaggcgttg aaggggtgac ctctgtcagc
     5341 gacaaactgc acgttcgcga cgctaaagaa ggctctgtga agggctacgc gggtgacacc
     5401 gccaccacca gtgaaatcaa agccaaactg ctggcggacg atatcgtccc ttcccgtcat
     5461 gtgaaagttg aaaccaccga cggcgtggtt cagctctccg gtaccgtcga ttcccaggca
     5521 cagagcgacc gtgctgaaag tatcgccaaa gcggtagatg gtgtgaaaag cgttaaaaat
     5581 gatctgaaaa ctaagtaatt cgtcgtaatt cgtcctcccg aactttgtcg ggaggcgcga
     5641 tgtgcaccac actaaaaata tcgcattgag tgattttcac gctcatatca agcggtgaca
     5701 ttaactatgg taaaggagac gcttatgttt cgttggggca tcatatttct ggttatcgcg
     5761 ttaatcgccg ccgcacttgg gtttggtggt ctggccggta ccgctgcagg cgcagctaaa
     5821 attgtctttg tcgtcgggat tattctgttc ctggtgagtt tgttcatggg ccgaaaacga
     5881 ccctagattt cagaccatta agaaaatttt atccaaagcc agtccagcgg actggctttt
     5941 gcggttttag cgaataataa attgcgttac tttgtcattc tttcacaaca aaaacaggaa
     6001 ggcagaggtg gggcagcgaa tacctgtaac gcttggtaat attgcgccgt tgtcgctaag
     6061 gccgttccag cctggacgaa tagctctggt gtgcgaaggc ggcggacagc gtggaatttt
     6121 cacggctggc gtgctggatg agtttatgcg cgcgcagttt aatcctttcg atctttatct
     6181 cggcacatct gccggggcgc agaacctctc ggcgtttatc tgcaatcagc ccggttacgc
     6241 gcgcaaagtc atcatgcgct ataccacaaa acgcgaattt ttcgatccat tgcgctttgt
     6301 ccgtggagga aatcttatcg atctcgactg gctggtggag gccaccgcaa gccagatgcc
     6361 gttgcaaatg gataccgccg cgcggttgtt tgacagcggc aaatcgtttt atatgtgcgc
     6421 ctgtcgtcag gatgactacg cgcctaatta ctttttacca accaaacaaa actggctgga
     6481 tgtgattcgc gcctccagtg cgatacctgg cttttatcgt agcggagtgt cgctggaagg
     6541 cattaactac ctggatggcg ggatcagtga tgcgattccg gttaaagagg cggcaaggca
     6601 gggcgctaaa acgttggtcg tcattcgcac tgtgccgtca caaatgtact acacgccgca
     6661 gtggttcaaa cgcatggaac gctggctggg tgacagtagc ctgcagccgc tggtcaatct
     6721 ggtgcagcat catgaaacca gctatcgtga cattcagcaa tttattgaga aaccaccggg
     6781 caagctgcgg atattcgaaa tttatccgcc gaagccatta catagtatcg cgcttggcag
     6841 tcggattccg gcgctgcgtg aagactataa acttgggcgt ttatgcggtc gttatttcct
     6901 cgccacggtt ggcaagctat taactgaaaa agcgccgctt acccgccatc tggtgccagt
     6961 ggtgacgccg gaatcgatcg ttattccgcc tgcgccagtc gccaacgata cgctggttgc
     7021 cgaagtgagc gatgctccgc aggcgaacga cccgacattt aacaatgagg atctggcttg
     7081 atttgccgtt ttatcgatac ccactgccat tttgatttcc cgccgtttag tggcgatgaa
     7141 gaggccagcc tgcaacgcgc ggcacaagcg ggcgtaggca agatcattgt tccggcgaca
     7201 gaggcggaaa attttgcccg cgtgcaggcg ttagcggaaa aatatcaacc gctgtatgcc
     7261 gcattggggt tgcatccggg gatgttggaa aaacatagcg atgtgtctct tgatcagcta
     7321 cagcaggcgc tggaaaggcg tccggcgaag gtggtggcgg tgggggagat cggtctggat
     7381 ctcttcggcg acgatccgca atttgagagg cagcagtggt tactcgacga acagctgaaa
     7441 ctggcgaaac gctacgatct gccggtgatc cttcattccc ggcgtataca cgacaaactg
     7501 gcgatgcatc ttaaacgtca cgatttaccg tgcactggcg tggttcacgg tttttccggc
     7561 agcctgcaac aggccgagcg gtttgtacag ctgggctaca aaatcggcgt aggcggtact
     7621 atcacctatc cacgcgccag taaaacccgc gatgtcatcg caaaattacc gctggcatcg
     7681 ttgttgctgg aaaccgacgc gccggatatg ccgctcaacg gctttcaggg gcagcctaac
     7741 cgaccggaac aggctgtgcg ggtgtttgac gtgctttgcg agttgcgccc ggaaccagaa
     7801 gatgagattg ccgaagtgtt gcttaataat acgtatgcgt tgtttagcgt tagtgggtag
     7861 cctacccttc gtaggcagca tccggcagtc ggcgcataat gcctgatgcg acgcttaacg
     7921 cgtcttatca tgcctacagg tttgtgccga accgtaggcc ggataaggcg ttcacgccgc
     7981 atccggcagt tggcgcacaa tgcctgatgc gacgcttgac gcgtcttatc at
//
LOCUS       DAJTYZ010000163         7987 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000163 DAJTYZ010000000
VERSION     DAJTYZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.163"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="OO596_003625"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="OO596_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR6719392.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="OO596_003626"
     CDS             complement(2975..3697)
                     /locus_tag="OO596_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6719393.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="OO596_003627"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="OO596_003627"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6719394.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>7987
                     /locus_tag="OO596_003628"
     CDS             6679..>7987
                     /locus_tag="OO596_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCR6719395.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTL"
BASE COUNT         1926 a         1977 c         2279 g         1805 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgc
//
LOCUS       DAJTYZ010000164         7959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000164 DAJTYZ010000000
VERSION     DAJTYZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.164"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..239
                     /locus_tag="OO596_003629"
     CDS             <1..239
                     /locus_tag="OO596_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCR6719396.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="OO596_003630"
     CDS             279..1142
                     /locus_tag="OO596_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR6719397.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="OO596_003631"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="OO596_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCR6719398.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAVLKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="OO596_003632"
     CDS             2679..4037
                     /locus_tag="OO596_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR6719399.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="OO596_003633"
     CDS             4180..5130
                     /locus_tag="OO596_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCR6719400.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="OO596_003634"
     CDS             5140..6522
                     /locus_tag="OO596_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR6719401.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="OO596_003635"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="OO596_003635"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCR6719402.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1916 a         2016 c         2306 g         1721 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggtgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaata
//
LOCUS       DAJTYZ010000165         7821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000165 DAJTYZ010000000
VERSION     DAJTYZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.165"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            60..476
                     /gene="hicB"
                     /locus_tag="OO596_003636"
     CDS             60..476
                     /gene="hicB"
                     /locus_tag="OO596_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCR6719403.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            555..1961
                     /locus_tag="OO596_003637"
     CDS             555..1961
                     /locus_tag="OO596_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR6719404.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2206..3351
                     /locus_tag="OO596_003638"
     CDS             2206..3351
                     /locus_tag="OO596_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6719405.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3369..4382
                     /locus_tag="OO596_003639"
     CDS             3369..4382
                     /locus_tag="OO596_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6719406.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4383..5283
                     /locus_tag="OO596_003640"
                     /pseudo
     CDS             4383..5283
                     /locus_tag="OO596_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5273..6067
                     /locus_tag="OO596_003641"
     CDS             5273..6067
                     /locus_tag="OO596_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6719407.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6089..7513
                     /gene="patD"
                     /locus_tag="OO596_003642"
     CDS             6089..7513
                     /gene="patD"
                     /locus_tag="OO596_003642"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6719408.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            7723..>7821
                     /locus_tag="OO596_003643"
     CDS             7723..>7821
                     /locus_tag="OO596_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCR6719409.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKI"
BASE COUNT         1839 a         1844 c         2148 g         1990 t
ORIGIN      
        1 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
       61 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
      121 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
      181 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
      241 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
      301 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
      361 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
      421 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
      481 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
      541 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
      601 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
      661 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
      721 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
      781 cgccagtcat tccagtcact cgagatgaag cagtcgatat caacacttat atttttgata
      841 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
      901 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
      961 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     1021 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     1081 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     1141 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     1201 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     1261 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     1321 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     1381 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     1441 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     1501 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     1561 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     1621 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     1681 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     1741 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     1801 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     1861 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     1921 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     1981 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     2041 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     2101 tctcagggac gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     2161 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     2221 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     2281 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     2341 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     2401 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     2461 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     2521 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     2581 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     2641 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     2701 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     2761 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     2821 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     2881 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     2941 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     3001 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     3061 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     3121 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     3181 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     3241 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     3301 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     3361 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc
     3421 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     3481 cgtccggctc cggcaaaacc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     3541 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     3601 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     3661 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     3721 cgctggagaa agtggcgttg gggtttgtac agcaacgtaa accgtcacaa ctttctggtg
     3781 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     3841 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     3901 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     3961 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     4021 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccgggttc gttggtacat
     4081 cgaatgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     4141 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     4201 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     4261 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     4321 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     4381 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctggggctg tttttactgc
     4441 tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg acactgttat
     4501 ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg
     4561 cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta
     4621 tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg
     4681 cgcgctatac cagcgggaaa atgaaagcgt ttttatattg cggtaatgtt gccgatgtgg
     4741 gcgagctaca ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct
     4801 cagtggtttt tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct
     4861 gcggtgggcg gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat
     4921 atctggttgc cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca
     4981 ttgttgcagg cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg
     5041 ctgccgctgg caatcccggg tattgccgct ggctctatct ttaccttctc actcactctg
     5101 ggcgatttta tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt
     5161 tattcccagc agggggcgat tggcaatatg ccgatggcgg cggcattcac cttggtgccg
     5221 attattctca tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc
     5281 tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt
     5341 tcccatcctg ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc
     5401 accgcagggc ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga
     5461 tgccgtgaca ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg
     5521 gacgctggca gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct
     5581 gttactgctg ctgcccattg cgctgccggg cagcgtcact ggtctggcgt tattaaccgc
     5641 ctttaaaacc atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt
     5701 ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct
     5761 ggttgaggcg tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt
     5821 gccgaatctc agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga
     5881 tgaaatcatc gttacgacct ttacggcagg tcatgaacga acgttactgt tgtggttgct
     5941 caatcagctt gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat
     6001 gttggtaaca accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg
     6061 tcaataacca ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact
     6121 ggttagcggc gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact
     6181 ggaaattgcc gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc
     6241 atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc
     6301 tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa
     6361 accgctgcat agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt
     6421 cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac
     6481 ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc
     6541 gctgatgatg gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct
     6601 taaaccatca gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat
     6661 cttcccggca ggcgtgatta acatactgtt tggcagaggc aaaacggtgg gtgatccgct
     6721 gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca
     6781 catcatcagc catgccgcgt cgtccattaa gcgtactcat atggaacttg gtggcaaagc
     6841 gccagtgatt gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt
     6901 tggctattac aatgctggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg
     6961 catttacgat acgctggtgg aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc
     7021 gccggatgac gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt
     7081 cagcaaggca gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga
     7141 aaagcgcaag ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga
     7201 cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc ccttcgacaa
     7261 cgaagaacag gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg
     7321 gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg
     7381 ggtcaatacc catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg
     7441 ttacggcaag gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat
     7501 ggttaaacat taagttagat gttagtggct gttataggac ggccactatc gttttaggtt
     7561 ctgagtttaa tggcggttaa gtattagaat gattacatta ataactagcg taaaaattag
     7621 gctaaatgtt tgatggcgat ttttcatcct gtcacagttg attagcataa ggctttagag
     7681 tatcgttatt aaactaataa tttataagta gaagtattaa caatgaaagt tcaattgtac
     7741 aaattcacgg aagataaaaa taaaaccctt accttcagat ggacgaaaaa acactttgag
     7801 ttttgtatgg ataataaaat c
//
LOCUS       DAJTYZ010000166         7805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000166 DAJTYZ010000000
VERSION     DAJTYZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.166"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(323..1159)
                     /locus_tag="OO596_003644"
     CDS             complement(323..1159)
                     /locus_tag="OO596_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6719410.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1159..1962)
                     /locus_tag="OO596_003645"
                     /note="aph(3'')-Ib"
     CDS             complement(1159..1962)
                     /locus_tag="OO596_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6719411.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(2023..2838)
                     /gene="sul2"
                     /locus_tag="OO596_003646"
     CDS             complement(2023..2838)
                     /gene="sul2"
                     /locus_tag="OO596_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6719412.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            4027..4176
                     /locus_tag="OO596_003647"
     CDS             4027..4176
                     /locus_tag="OO596_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719413.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(4932..5372)
                     /locus_tag="OO596_003648"
     CDS             complement(4932..5372)
                     /locus_tag="OO596_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6719414.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            5590..5979
                     /locus_tag="OO596_003649"
     CDS             5590..5979
                     /locus_tag="OO596_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719415.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            6023..6211
                     /locus_tag="OO596_003650"
                     /pseudo
     CDS             6023..6211
                     /locus_tag="OO596_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6178..7377)
                     /gene="tet(A)"
                     /locus_tag="OO596_003651"
     CDS             complement(6178..7377)
                     /gene="tet(A)"
                     /locus_tag="OO596_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR6719416.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            7483..>7805
                     /gene="tetR(A)"
                     /locus_tag="OO596_003652"
     CDS             7483..>7805
                     /gene="tetR(A)"
                     /locus_tag="OO596_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR6719417.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAP"
BASE COUNT         1796 a         2124 c         2135 g         1750 t
ORIGIN      
        1 agggcggccg tcttcacgtt ctgccttgcg cagatgagat agatctagcg tggactcaag
       61 gctctcgcga atggctcgcg ttggaaactt tcattgacac ttgaggggca ccgcagggaa
      121 attctcgtcc ttgcgagaac cggctatgtc gtgctgcgca tcgagcctgc gcccttggct
      181 tgtctcgccc ctctccgcgt cgctacgggg cttccagggg tcgtttgcgg gaaggggcgg
      241 aatcctacgc taaggctttg gccagcgata ttctccggtg agattgatgt gttcccaggg
      301 gataggagaa gtcgcttgat atctagtatg acgtctgtcg cacctgcttg atcgcggccg
      361 cgatagctag atcgcgttgc tcctcttctc catccgcgtt ccaagctgcg gaaaggcacc
      421 cataagcgta cgcctggtcg agcaggcgac gcggatcgac gtccagcgca cgagagaatg
      481 cgtccgccat ctgtgcaatg cgtctaggat cgagacaaag gtcgtctctg tcagccggat
      541 cgtagaacat attggcggcg ccaaagccca cttcaccgac cagaccgacg ggatctatca
      601 ccagccagcc gcgactggag aacatgatgt tttcatgatg cagatcgcca tgtagcccac
      661 gcagttccga ggcattgctc atcatttgat cggctataat cgccgcgtgg acgtagtcag
      721 tttgacaacc tgcgttttga tcatcgcgcg cccgctgaaa caaagctgca aagcgatccc
      781 ggatcgggag aagggcagaa ggcaggggtt cctcagatgc ggcatacagc ttcgccatta
      841 gttccgctgc aatttcggtc gcctggtagt cgccgtgctc ggcaacgatg tgagagagca
      901 ttcgctcccc ggcatattcg agcaacatca gattgttctc acgaccgagc aaccggactg
      961 ctcccctccc attgcgccat accagatagt cggccccgcg cagttcatca gcaatgtctt
     1021 ctataggttt caatcccttg acgattgcag gagtcccgtc tggcaatgaa actttccaaa
     1081 cgaggctgga aaaggtgtcc gcaatgagaa caggttgcga aacgtgccaa tgagcaggaa
     1141 aaacaggcgg catgaacatc aaccccaagt cagagggtcc aatcgcagat agaaggcaag
     1201 gcgttcgcgg tcgggggctt cgatccccaa tacattgaat aggacagcga aggcgcgctc
     1261 tgcttcatct ggcgctgccc agttctcttc ggcgttagca atcatgagtg ccaaatcggc
     1321 atagcgatct gctgttccga gccgcccaag gtcgatcaga cccgtgcatt gaagagtttt
     1381 agggtccacc atgaagttcg gcatgcaggg atcaccatgg caaacaacca tatcggtgcg
     1441 ctcttggtcg agccgcaccg gtagctctcg ttcgacacga gccaaaagat cgagctgcgg
     1501 cgtactcttg tcctcgtccg gtaagaagtc gggattgacg gcattgcggg acaccacatc
     1561 aacggcgcgt ccgaacattc gcgacagcct gcgctcaaac ggacattgat caaccgatag
     1621 gctgtgaaca gcgccaagtt gctgccccat tgacggccac gctttgagca aatccgctcc
     1681 agacagatca gccgccggta ctcccggaat tgccgttatc accaagcatg caccctcctg
     1741 ttcctcctgc cagttgatga cctcggggca agccacacct cgacctttga gccaaatgag
     1801 gcggtcacgc tctccagcga gctcaccgcg gcgggaagca ggtgcgattt tcgcgaaggc
     1861 atgcccgtca ccacgtcgaa aaacaaaatc accagattct ccgcctctga caggcaacca
     1921 gtcagaatgc gattcaccaa aaaaaatatt agttcgattc aatggaggtt ccttcagttt
     1981 tctgatgaag cgcgaatata gagaaatatc ccgaatgtgc agttaacgaa ttcttgcggt
     2041 ttctttcagc gccgccaata ccgccagccc gtcgcgcaag gggcgcggct cgtgtgtgcg
     2101 gatgaagtca gctccacctg cggcggcggc aagctctgca gcgagtgtcg cggccccgac
     2161 atcccccgga ccacggcctg tgagcgcgcg cagaaaggat ttgcgcgaaa cagacagaag
     2221 caccggcaaa tcgaagcgca gccgcaattc atcgaaccgc gccagcaccg agagcgaggt
     2281 ttcgggagca gcccccagaa aaaaccccat gccgggatca aggacaaggc ggttgcgttt
     2341 gataccggca cccgtcagcg ccgcgatgcg cgcgtcaaag aacgccgcaa tgtgatccat
     2401 gatgtcgcca gcgggtgcct cgcgccgatc tgcctgcccg tcttgcaccg aatgcataac
     2461 gacgagtttg gcagatgatt tcgccaattg cggatagaac gcagcgtctg gaaaaccgcg
     2521 aatatcattg agataggcca caccacgcga caaggcatag gcttgcgtcg cgggttgata
     2581 actgtcgagc gagacgggaa tgccatctgc cttgagcgcg tccagcaccg gcgcgatacg
     2641 cgcgatttct gtgtcggacg aaacaggcgc ggcgtcggga ttgctggatg ccggaccgag
     2701 gtcgatcaca tctgccccct cggccatcag cttacgcgcc tgcgcaatgg ctgcgtctgg
     2761 cgccagatac cggcctccat cggagaaact gtccgaggtt atgttgacga tgccgaaaat
     2821 gatgagcgat ttattcatgg gggcttctat aataataata atcgagcatg agtctcatac
     2881 ggatgctcgg gtcgaaaggg aatccccagg cgagtaacct gtttgcggtg atccgtttca
     2941 tgatgatgta tctgtagcat tttggaaata aatatatcat ctctcaataa gtagatatat
     3001 ttatcttttt tgcttctttg gtgctgcaag tcaccaatac gagggaaacc tgttacggag
     3061 cggtttaacc gtaatcaaca gtgactggtg tcatgttcgg aagattaatt gatagtaatc
     3121 tacaaaggat cttcttgaga tccttttcaa tgaatcgtaa tctcttgctc tgtaaacgaa
     3181 aaaaccgcct ggggaggcgg tttgttcgaa ggttaagtca gttggggaac tgcttaaccc
     3241 ggtaacaggg ttcacgagac cgccattacc aaaactgttc tttcagtgta gccgcagtca
     3301 ggcctctact tcaagacctc ttaatctatc ttctcgtgca ctcctgttac taatggctgc
     3361 tgccagtggc gttttgtcgt gtctttccgg gttggactca agttgatagt taccggacaa
     3421 ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     3481 cttcacggaa ctgagtgtca ggcgtggaat gagaaaccgc ggccataaca gcggaatgac
     3541 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagctaccag ggggaaacgc
     3601 ctgggatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agattttgcg
     3661 atgcttgtca ggggggcgga gcctatggaa aaacggcttc gccgtggcct tatctcttct
     3721 ttgctaagtg tcttcctggt atcttccagg aaatttccgt ccccgtccgt aagccggtac
     3781 cgctcgccgc agccgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatct
     3841 cctgtatcgc atattctgct gacgcgccgg tgctgccttt tttctcctgc cacatgaagc
     3901 acttcactga ttttcgcaat cgtggcaaca tagtaagcca gtatacactc cgctagcgct
     3961 acatgactgg ttcagggctg cgccccgaaa cccgctaaca acctctcagc tactggatat
     4021 caccccatgc catctgaata tgatgaacaa ctggattcga ctgatacgca gcttcccggc
     4081 gaatatgacc tttttgattt aatggcgcag gttactgaag agaacctgca tgcccctgtg
     4141 gattttgggt cgcctgtcgg ccgggaagcg ttatagcttt ttcgcttgcc cgcgtacgtg
     4201 gtaatactcc ggtattacct tttatttctt tattttcatg taattacagt ggatttttaa
     4261 tacccgggta ttactcgtga aaacgaaata ttcctttttt ttcttttaat tcatgcagtt
     4321 gcgatgaaaa tcgcggctgc gttaggtgta tgaccatttt gaggttgaaa aataatcaac
     4381 catggcgggt gatttacgca ggttaagggg agatcgtgac aaaaaaagcg ttcctcactg
     4441 tatcgttcat gcctgcatga acgggttatt cttcctgact acagaattac atcgctcctt
     4501 cagttcatac atcacttata catcactttt acatcactta tacatcactt ttacatcatt
     4561 ccttcagctc atacgtcact ttcatcctct gtgccatttg tacatcaccc ccttcctgaa
     4621 cgtgctgaca gcgggttttc cggctgcgcc ggcaacgctt cacggccacg caataaattg
     4681 cgtccccgtt caccgttttc ggatgggaag aacgccggaa acaggactta ccccggctga
     4741 atggtgtgaa tttccgcgct atgcacttgt gcgcatactc atgcatgccg taaaaacaga
     4801 gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc tgttttaccg gtttcccgtc
     4861 agaaacgccc tgagggcctc tcaggcggtg ctgataacag atgttatggt aaatcagagt
     4921 cacctgcact gttactctga accggagcga aacagccgcg accagaatcc ccgctttttt
     4981 tcctgtgcca gcgcgtcagc cagacggcta atttcggcct gtagttgttc cctctcctca
     5041 tttcgcaggc ggttttctgc ttcccgcgcc atcttgtctt caagcattag ggtaacagtc
     5101 tgctgcagag cgtccagttt ctgctgaatg acgtctagct gagcattttc cgcctggcta
     5161 acgacagatt tggagtgtcc ctccttgtgc ctttttggtg tctcagaagt gcgcagctcg
     5221 ccataggccc gaatcagttc actggtctcg aactcccggc gatcattttt tcctgtgcga
     5281 tagctgacaa ggcctttagc catgtcacga tataattgcg agcgcgactt tcctgttaat
     5341 ttcagtgtct cttgaactgt gtgccatgcc ataaaagtga cagtgtctcc tgtgtgtacc
     5401 gtattgtaac agcagaacat attactgttt taacacaaaa ggtctgaggc tgcccgtgag
     5461 gcgtgaaaaa tacacaaaaa acctgttgac ggtgtggttt tgacgggcgt agtatttcga
     5521 atactgtata taaacacatt gttataaata cagtttttgc tgtttttaaa agtcaaagag
     5581 gataatttta tgagtacaga gttaaatagc ggtactgtcg gtggttttgg tcacatggga
     5641 ccaaatgacc cctacaatga tggtcatagc ggctctggaa gttcgggagg tagttttaac
     5701 ggtggtggta atggtggcca tgataatagt agtggttcat cttatactgc atcctttggt
     5761 ctcatcactg gccgacaccc gataactgtc ccaggtacga agaatattaa ttctgtcgat
     5821 ctttctgctg tcattaataa cattatacat catgaaggtg cacgcaataa catgtaccta
     5881 gatactgcga acaatgtaag agtgggagtg acgggcactg gctggcaatg tctagcaacg
     5941 gcaggcattt cggctgaggg taaaagaact ttccgctaag cgatagactg tatgtaaaca
     6001 cagtattgca aggacgcgga acatgcctca tgtggcggcc aggacggcca gccgggatcg
     6061 ggatactggt cgttaccaga gccaccgacc cgagcaaacc cttctctatc agatcgttga
     6121 cgagtattac ccggcattcg ctgcgcttat ggcagagcag ggaaaggaat tgccgggcta
     6181 tgtgcaacgg gaatttgaag aatttctcca aagcccgcga cgcagcgccg gcaggcagag
     6241 caagtagagg gcagcgcctg caatccatgc ccacccgttc cacgttgtta tagaagccgc
     6301 atagatcgcc gtgaagagga ggggtccgac gatcgaggtc aggctggtga gcgccgccag
     6361 tgagccttgc agctgcccct gacgttcctc atccacctgc ctggacaaca ttgcttgcag
     6421 cgccggcatt ccgatgccac ccgaagcaag caggaccatg atcgggaacg ccatccatcc
     6481 ccgtgtcgcg aaggcaagca ggatgtagcc tgtgccgtcg gcaatcattc cgagcatgag
     6541 tgcccgcctt tcgccgagcc gggcggctac agggccggtg atcattgcct gggcgagtga
     6601 atgcagaatg ccaaatgcgg caagcgaaat gccgatcgtg gtcgcgtccc agtgaaagcg
     6661 atcctcgccg aaaatgaccc aaagcgcggc cggcacctgt ccgacaagtt gcatgatgaa
     6721 gaagaccgcc atcagggcgg cgacgacggt catgccccgg gcccaccgga acgaagcgag
     6781 cgggttgaga gcctcccggc gtaacggccg gcgttcgcct ttgtgcgact ccggcaaaag
     6841 gaaacagccc gtcaggaaat tgaggccgtt caaggctgcc gcggcgaaga acggagcgtg
     6901 gggggagaaa ccgcccatca gcccaccgag cacaggtccc gcgaccatcc cgaacccgaa
     6961 acaggcgctc atgaagccga agtgccgcgc gcgctcatcg ccatcagtga tatcggcaat
     7021 ataagcgccg gctaccgccc cagtcgcccc ggtgatgccg gccacgatcc gcccgatata
     7081 gagaacccaa aggaaaggcg ccgtcgccat gatggcgtag tcgacagcag cgccggccag
     7141 cgagacgagc aagaccggcc gccgcccgaa acgatccgac agcgcgccca gcacaggtgc
     7201 gcaggcaaat tgcatcaacg catacagcgc cagcagaatg ccatagtggg cggtgacgtc
     7261 gttcgagtga accagatcgc gcaggaggcc cggcagcacc ggcataatca ggccgatgcc
     7321 gacagcgtcg agcgcgacag tgctcagaat tacgatcagg ggtctgttgg gtttcacgtc
     7381 tggcctccgg accagcctcc gctggtccga ttgaacgcgc ggattcttta tcactgataa
     7441 gttggtggac atattatgtt tatcagtgat aaagtgtcaa gcatgacaaa gttgcagccg
     7501 aatacagtga tccgtgccgc cctggacctg ttgaacgagg tcggcgtaga cggtctgacg
     7561 acacgcaaac tggcggaacg gttgggggtt cagcagccgg cgctttactg gcacttcagg
     7621 aacaagcggg cgctgctcga cgcactggcc gaagccatgc tggcggagaa tcatacgcat
     7681 tcggtgccga gagccgacga cgactggcgc tcatttctga tcgggaatgc ccgcagcttc
     7741 aggcaggcgc tgctcgccta ccgcgatggc gcgcgcatcc atgccggcac gcgaccgggc
     7801 gcacc
//
LOCUS       DAJTYZ010000167         7732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000167 DAJTYZ010000000
VERSION     DAJTYZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.167"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..400)
                     /locus_tag="OO596_003653"
                     /pseudo
     CDS             complement(1..400)
                     /locus_tag="OO596_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(586..3894)
                     /gene="icsA"
                     /locus_tag="OO596_003654"
                     /gene_synonym="virG"
     CDS             complement(586..3894)
                     /gene="icsA"
                     /locus_tag="OO596_003654"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6719418.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGNGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVVGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQNEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4423..5625
                     /gene="virA"
                     /locus_tag="OO596_003655"
     CDS             4423..5625
                     /gene="virA"
                     /locus_tag="OO596_003655"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6719419.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASNSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5804..6491
                     /locus_tag="OO596_003656"
                     /pseudo
     CDS             5804..6491
                     /locus_tag="OO596_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    6084..6200
                     /locus_tag="OO596_003656"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            6493..6859
                     /locus_tag="OO596_003657"
                     /pseudo
     CDS             6493..6859
                     /locus_tag="OO596_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6787..7260
                     /locus_tag="OO596_003658"
                     /pseudo
     CDS             6787..7260
                     /locus_tag="OO596_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT         2274 a         1785 c         1539 g         2134 t
ORIGIN      
        1 gttcagttca ggctgtacgc ttccccatgt aatttgcctc gccacttcac cgggctcagg
       61 accccgtagg atcttcaccg aaaaaaatgc atcaccagtt gctttattat ttgttttagg
      121 tagacctgtt actaatttat acctactctg accaagtctg gataagaaag gcacacctgc
      181 acatttctac ttggaaatgt tttcttttac cattttgctc aaaaatttca acatccaata
      241 ttcctcgtac agaactactc aagtcctgaa tagaaaaagt tccagctggt acagtagagt
      301 catatattac tcgaccttgt tgagatacaa caacacgtgc attagtttct gcaataccag
      361 taatctgagg cgcataacca cgaatatttg gcggtagcat tcgttcatcg ctttctaatg
      421 caattcctgt gtaacgccaa gcaacagcgc attctggcgc atgatctggt aaacacgttt
      481 ggcattgatc gcaggcatac catcatgtgc acaaaaaccc accgagcttt tacacggtgg
      541 gtcccagaga aatgcaggac atcaacacgc cctgcatttt tattatcaga aggtatattt
      601 cacacccaaa ataccttggg tgtctctgta actgttatta cccaattgct gagatacgtt
      661 tccccagatg cttaggttat ctgcaagacg cccccccaca ccagttttga gttcaatcac
      721 attacggttg cctatctggt gatttgacat gccattcata attacaccat attgctttga
      781 gctccatttc caattcactt ccaaaaatgg ttcgaactta cgaagagtct ccttatcgat
      841 acaactctga ctaattaagg atgtacgcaa tcccaatttt gcttgaatat tattgacccc
      901 agaggatgtg accatagtgc cattgtgttc aatatagtcg tttgctttta ccccatgcct
      961 agttatctgg acttgtggat tgagatacaa tgcgttgtca atattattat gagcagtcca
     1021 gcgtatggtt gggtagatat agccagcttc caaagctcct gttattcctt tgctggaata
     1081 tttctcacca gttaacccat ctcctttgac tgtattatta aaccaaccgt actgcaacca
     1141 agcatccata tatagacctg ttcgttcttt ttcattctga aaccaagaac tgtacaaacc
     1201 ggcagtataa ccgctcacag ttcctcgtga actataacta gtatacttat tatgagtttt
     1261 gctatcctga tatccataag ctcccataat acccacatgc aagccatcat gatcagtgaa
     1321 gtttgtcttc aaaatatctc caccaatctg ataaaccatc ctgttagctg tggtttttaa
     1381 ttgtctatca gctaaattat gctcattatg tccaccaaca gtacgaatcc acatggtggt
     1441 ttcgctgagc tgacctgtag taggatcaac aatagtgcta ccgtgtcggt catagagaga
     1501 aggactaaac atcgtgttca ttgctatcag gttagcaata taactgccag cttcagggcg
     1561 ccatgtgtga atactacctt cacccaaatt aagtgaagat acaagctgtg aactagctcg
     1621 gcgactactc atttgagtag actcttgatt gctcatctgt tttgattctt gattatccat
     1681 ctgactagtt agataccact tattggtatt cagtccagat acagtgccct gtttcaggcg
     1741 atagtcataa ctaccagcaa caatacggcc tttctgaata aaagcattct tatcagaaga
     1801 atctgtcgaa ataatttgaa caccttcaag agttttatct ccagagcctc cctcatttgt
     1861 cactgcaaca taggtaatac cagacgcatg gcctgcaatt tccagccggt cagttttact
     1921 gttgtcattg ccgagaaccg cgcccaaaga taatattccg ccctttccat gccaattacc
     1981 tttctgaaca taggtctgac cgacatttga cgaactatta ttgagaataa catgaccgtt
     2041 tttctccaga gtcatgttac ctgtgacaat gacggggtca ggcaaaaaag gggcattaat
     2101 attattgatt agcacagtcc cactatttaa taaagtgtta acagtttggt tcatgcctga
     2161 aaaattcaag gtagcacctt tattaacaat aacaccagcg gtacgtgtca tagcttcaac
     2221 tgtccccatt tttagaatgc ctgcattgat gttggtgaaa ccagagtagg tatttttttc
     2281 cgccaaaatg agagttccct cccctgattt tgttagggat tttccgtccc agctatttat
     2341 attatgatgg ctgagattat ctgccagaat agtactaaca gtaagttcag cgttttcttt
     2401 cagattgaac tctccatgac tgtcgccatc tgtatcattc cagctcaaac cgtagatcaa
     2461 tttattctgg tcactgatat agctttttgt atagtcaata gcaccagtag tgagtaacgg
     2521 attgatgata gatatattag aaacactgaa acctgttatc cctttatctg cacttagcag
     2581 tttatagtcc ttctgcgccc agttatttat aatagcttcc acttttacat tgttgccaaa
     2641 tgacatattt ccagaagctt ccagactact gattccagct aaatttaaag aactgttctc
     2701 accaaaagaa acgtcacctg aaacagtgaa tttgttacct gatagtgaaa cagttgtaca
     2761 atcaccagca cataaatcac cttcaatagt tggtgtggtc ccagtgtaat cattgttgat
     2821 aattttcaga atggatgaat tgttaacctg tacatcacca gtaatgacag aaccttcatg
     2881 gagtattaat gaattgtttc caccagtgat gtggatagca ttacctacta cattttgtgt
     2941 tccatcatct tctttacctg aaattatata tccgctatta gcaatggtca tattgctacc
     3001 atttatagca tccccccaat gtccaccatt accgcctaaa atagctccat tgttgattat
     3061 agacaggtta tctcctgtga tagcattacc accgtagcca tcataaccat aaccgtaagc
     3121 agcacctccg ttgcctccag cgaacgtgcc cttattgatt acagagaggc tgcttcctgt
     3181 gatggcatca cctccattac cgccatcacc ctcaccataa ttgttacctc cattaccacc
     3241 ggaaatagtc cctttgttga ttatgggtag attgactcct gtgacggcat cacctccatt
     3301 ccccccatca ctatcactat aactatcgcc accatgtcca cctgaaataa tttctccatt
     3361 tattataaac agattgtctc ctgtaacagc ctcaccacca tcaccgttat ggtcagcacc
     3421 gctaccgccg ttaccaccaa gaatcatgcc ttgattgatt atagacaagt cagatccggt
     3481 aatagagtca ccaccattac cgccacaact attaccatca ttattatcac cagcaccacc
     3541 atgacctgcg ctaatcataa tatcagagtt aatccgtagc ttagttctag atgcatgaga
     3601 ggggttggaa gaagttatat accacgcatc cattccatct tcaccagctc ccaagggaga
     3661 aagtccatca acaggtgtta ataatttttc ataattgtcc tctgaaaaat gaagttcttg
     3721 agtacccgaa agaggaatag caaaagctat tggccccccg agcaacaggg atgcaccaac
     3781 aacaaaagga gaaaaagaca atttgcatgt tttttccttt accgtaggta attctccggc
     3841 ccccccctgt gaacattggg tcatattaca aaaaaatttg tgaatttgat tcatgcacta
     3901 tattatcagt aagtggttga taaaccccta aaaagattcc gttttatccg gaataaaggg
     3961 acgatatatg caaaaatata ttaaacaaag cctcaaccaa aacatgagag ctgtgttata
     4021 tatcaatgca acgggagaaa tttacctgtg tcttgctttg aaaaaaagag ggggaaagct
     4081 atatgttcga taataaatta ataattttac tatgatagtc tgcttcagac cgcaactttt
     4141 cagttctttc tgtacgcttg cccaaagttt aaccactttg tttttctata cacccaatgg
     4201 ttctatctgc cccttgatgt taatccccaa agccaataac atgaatgtat tcatatcaca
     4261 actttcctac cagactctcc aatgcaataa ctgcataatt gtttttagtg gaaatgtatc
     4321 gttttcttaa agagaagaat aacattccat tttattatgt tcccaattta attatattaa
     4381 tatatattgg cattaatagg aaaatacatc aggagaaatc aaatgcagac atcaaacata
     4441 actaaccatg aaagaaatga ctcatcatgg atgtcaactg tcaaatcaac tacagaagta
     4501 agttggaata aattgagttt ttgtgacgtt ttactgaaaa taataacttt cggcatatat
     4561 tctccacacg aaactctagc cgaaaaatat agcgaaaaaa aactaatgga tagtttctca
     4621 ccatcactat cacaagataa aatggacgga gagtttgccc acgcaaacat agatggcatt
     4681 tcaatacggc tatgccttaa caaaggcata tgttcggttt tttatcttga tggagataaa
     4741 atacaatcca ctcaactttc atcaaaagaa tataataact tgttaagttc actccccccc
     4801 aaacaattca acttaggaaa agtacatact attacagctc cggtatcagg gaattttaaa
     4861 actcacaagc ctgcaccaga agttattgaa acagccatta attgctgtac atcgataata
     4921 ccaaacgatg attacttccc cgttaaagac actgatttta atagtgtttg gcatgatatt
     4981 tacagagata ttagagcatc taacagcaat agtacaaaaa tatatttcaa caatattgaa
     5041 ataccactaa aattaattgc agatctaatt aatgagttag gaattaatga attcattgat
     5101 agcaaaaaag agcttcaaat gctaagttac aaccaagtaa ataaaataat caattcaaac
     5161 ttcccccaac aagacctatg ttttcaaaca gaaaaacttt tgtttacttc actctttcaa
     5221 gaccctgcat ttatatctgc actaacatct gcattctggc aatctcttca catcacgtct
     5281 tcctctgtgg aacacatata tgcccaaatt atgtccgaaa acatagaaaa caggcttaat
     5341 ttcatgcctg aacaacgagt tattaacaat tgtggacata ttataaaaat caacgcagtt
     5401 gtccctaaaa acgacactgc aatctctgcc tctgggggga gggcttacga agttagctca
     5461 tcaatattac catcgcacat aacatgtaat ggtgttggta ttaataaaat agaaacaagc
     5521 tatctcgttc atgctggaac acttccaagc agtgaagggc ttcgcaatgc tatccctcca
     5581 gaaagtcgtc aagtatcatt tgccatcata tctcctgatg tttaagccaa tattatattg
     5641 gctgccagac tgtaaaaaaa cgttcttcag aacagctcta taaactatta aaccaaagct
     5701 aaaaggatgg ggaccaatag aatctcagcc catccttttt atatcattat gaaccgcccc
     5761 ggaaatcctg gagactaaac tccctgagaa agaggtaaac aggatgacta aaaatacacg
     5821 tttttcccct gaagtccgtc aacgggcagt tcgtatggtt ctggaaagtc agggcgaata
     5881 tgactcacaa tgggcagcga tttgttccat tgccccaaag attggctgta tactggagac
     5941 tctgcgtgtc tgggtacgcc agcatgagcg ggataccggg ggcggtgagg tgggctcacc
     6001 accgctgaac gtcagcgtct gaaagagccg gaacgtgaaa atcgtgaact gcgccgcagt
     6061 aacaatatcc ttcgcctggc ttccgcttat tttgcaaagg cggagttcga ccgcctctgg
     6121 aaaaaataat gccactgctg gataagctgc gtgagcagta cggggtcgga tcggtatgca
     6181 gtgaactgca tattgccccg tcaacgtatt accactgtca gcaacagcga catcatcatg
     6241 ataaacgcag tgcccgtgcg cagcgcgatg actggctgaa gaaagagata ctgcgcgtat
     6301 acgatgagaa tcatcaggta tacgctgtgc gtaaagtctg gcatcagttg ttacgggaag
     6361 gtatcagagt ggccagatgc actgtggcac gtctcatggc ggttatgggg cttgccggtg
     6421 ttctccgggg taaaaaggtc cacactaccg tcagccggaa agccgttgcc gcaggcgacc
     6481 gcgtaaaccg tcatcaaggc aatatgccgc gtactccggg tggcccgcag cggctggtat
     6541 acgtggtgtc agcggcggac aaggataaac acacgtcagc agttccgtca gcactgcgac
     6601 agcgttgtcc gcaaggcttt tacccagtcc agcgttacgg tgccccacgc ctgacggatg
     6661 aactgtgtgc tctggttacc actttaacgt aaaaaccgtg gcagccagtc tgcgccgtca
     6721 ggaactgagc gcaaaagcct cccagaagtt cagcccgatc agttaccgtg cacacggcct
     6781 gcctgtatca gaaaatctgc tgacgcagga tttttacgcc agtggcccga accagaagtg
     6841 ggcgggagac atcacgtact attacagctc accgactgcg gggaaacacg gggcgccggg
     6901 ttattgaagg tgacctgtcc agctacttcg acacagtaca tcaccggctg ctgatgaaag
     6961 ccgtctgccg caggatcagt gatgcacgtt ttatgagact gctgtggaaa acgccgtgtt
     7021 gatgttggtc tcttccgggc ggtcagtgaa agtatgccac agggcggtgt gatctcgccg
     7081 ttgctgtcga acatcatact gaatgagttc gaccagtatc tgaataaacg ctacctgagt
     7141 ggaaaagcca gaaaggatcg ctggtactgg aatcacagta tccagcgagg ccgaagtact
     7201 gctgtcaaag aaaactggca atggaaacct gccgttgctt actgttgcta tgccgatgac
     7261 tgtgtgccac gaaggcgcgt cctggggacg tgagtccctg tgcagccatg cggcacaaga
     7321 gatgagggaa ggcccctcgg tgtcggcgtg caggcgcagg caccaaacca tccactgctg
     7381 ctgcatttaa gagatgtggt ggtgagcgag atgggaaaag gtcggacgtg agccgatcag
     7441 gtatggttcg aagagacgaa cgaaagtgaa ccgctgatga tgtgtcgaaa gcatatagat
     7501 gatgtcaaaa caatggtgga gtcgttcctg tgggatatgt ttactgcctg cgcggcatcc
     7561 ggcataaaag cggcgtgaag tcgattcagg ctcttgtggg gaacgtggac ctgtcacccc
     7621 gatgtgaagg gagaagcgca agtggaagcc ccatgagcgc cagagtacca atgcggggta
     7681 cagggacgga gcaccaagcg attgcgcagt aaacatgctt gacaacttaa tt
//
LOCUS       DAJTYZ010000168         7640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000168 DAJTYZ010000000
VERSION     DAJTYZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.168"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(110..2488)
                     /gene="ppsA"
                     /locus_tag="OO596_003659"
     CDS             complement(110..2488)
                     /gene="ppsA"
                     /locus_tag="OO596_003659"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6719420.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            2821..3654
                     /gene="ppsR"
                     /locus_tag="OO596_003660"
     CDS             2821..3654
                     /gene="ppsR"
                     /locus_tag="OO596_003660"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6719421.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            3811..4857
                     /gene="aroH"
                     /locus_tag="OO596_003661"
     CDS             3811..4857
                     /gene="aroH"
                     /locus_tag="OO596_003661"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6719422.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            4989..5180
                     /gene="hemP"
                     /locus_tag="OO596_003662"
     CDS             4989..5180
                     /gene="hemP"
                     /locus_tag="OO596_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6719423.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5184..6620)
                     /locus_tag="OO596_003663"
     CDS             complement(5184..6620)
                     /locus_tag="OO596_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6719424.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6683..7396)
                     /gene="ydiV"
                     /locus_tag="OO596_003664"
     CDS             complement(6683..7396)
                     /gene="ydiV"
                     /locus_tag="OO596_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6719425.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         1939 a         2027 c         1816 g         1858 t
ORIGIN      
        1 gaaaagatat tatgcggcgg ttaactcagg atgtctgtga agagattgaa taagtttcat
       61 cttcggggat cacataaccc cggcgactga acgccgccgg ggatttattt tatttcttca
      121 gttcagccaa gcttaaccag gtttgcacca cggtgtccgg gttcagagac aggctatcga
      181 tcccctcttc catcaaccat gcggcaaagt cttcgtggtc ggacggaccc tgaccgcaaa
      241 tcccgacata tttgccctgt ttcttcgcgg cacggatagc catcgacagc agtgctttca
      301 ccgcatcgtt gcgctcatcg aacaattcag acaccacgcc ggagtcacgg tccagaccga
      361 gcgccagctg cgtcatatcg tttgagccaa ttgagaagcc gtcgaaatat tcgaggaact
      421 gctcggccag caaggcgttg gacgggattt cacacatcat gatgattttc agcccgttct
      481 caccacgctt cagcccctga cgcgccagtt cctcaaccac cgctttcgcc tgatctacgg
      541 tacgcacgaa cgggatcatg atctcaacgt tggtcagccc catgtcgttg cgcacacgtt
      601 tcactgcttc gcactccagc gcgaagcagt cgcggaagct gtcggaaaca tagcgtcccg
      661 cgccacggaa gccgagcatc gggttctctt catctggctc gtaacgctca ccaccgacca
      721 ggttggcata ttcgttcgat ttaaaatcag agagacggac aatgacgcgc ttcggataaa
      781 acgcggcacc cagcgtcgcg atcccttcag tcagacgacc aacgtaaaat tcacgcggag
      841 aatcaaaacc tttcatcatc tcgcggattt cattttgcaa ctgcggttcc tgatcgtcaa
      901 actcaagcag tgcgcgtggg tggacgccaa tcatacggtt gatgataaat tccagacgcg
      961 caaggcccac gccttcgttc ggcaggcagg cgaagtcgaa agcacggtcc gggttaccga
     1021 cgttcatcat cactttcaac ggcagatccg gcatcatttc tacgctggag cttttcacgc
     1081 taaattccag caactccgca tagacgtaac cggtatcacc ttcggcacaa gaaacagtga
     1141 cgttctcacc gtctttcatc cgttctgttg catcaccaca gcccactacc gccggaatgc
     1201 ccagttcacg agcgatgatc gccgcgtgac aggtacgacc gccacggttg gtgacgatgg
     1261 cagatgcttt cttcatgatc ggttcccagt ccgggtcggt catgtcagtg accagcacgt
     1321 caccaggttc gatgcggttc atttcgctga tctcatggat gactttcacc ggacccgcgc
     1381 cgatgcgatg accgatagca cggccttcgg cgataatctt accctgtgaa tgcagcgtat
     1441 aacgctccat gacctgaccg cgtgagcgca cggtttccgg acgcgcctgc acaatgaaca
     1501 gtttgccagt gtggccatct ttcgcccact caatatccat cgggcgaccg tagtgtttct
     1561 caatttgtac ggcctgtttt gccagttcct gcacttcttc gttggtcagc gagaagatgt
     1621 cacgctgttc ctgcggtacg tcttcgattt taacctgctt gccgtgctcc tgggtcggcg
     1681 cgtaaaccat gcggattttt ttcgacccca tggtgcgacg cacgatagcc gggcgattcg
     1741 ccgccagtgt cggtttatgg acgtaaaact catccgggtt aaccgcaccc tgcacgacca
     1801 tttccccaag gccccatgcg gaagtgataa acaccacctg gtcaaagccg gattcggtat
     1861 caatggagaa catcacgcca gaagatgcga ggtcggagcg caccatccgc tgaacaccgg
     1921 cagagagcgc cacgccacgg tgatcgtaac cctggtgcac acgataagag atggcgcgat
     1981 cgttaaacag agaagcaaat acatgcttca ctgccacgag aacggcgtca aaaccctgaa
     2041 cgttgaggaa ggtttcctgc tgaccggcaa aagaagcgtc cggcatatct tctgcggtgg
     2101 cggaggagcg caccgcaaaa gaggcgtttt cgtcatcggc ggaaagctgt gcataggctt
     2161 cgcggatggc gttttccagc tcaggctgga agggagtgtc gataatccac tggcggattt
     2221 gcgcgcccgc tttcgcaagc tgagtaacat cgtcaatatc cgttttatcc agcagttcat
     2281 aaatgcgctg gtttacgccg ctttggtcca gaaactggtt aaacgcgtcg gcggttgtgg
     2341 cgaaaccatt cggaacggaa acacccattc cggaaaggtt agtaatcatt tcacccaggg
     2401 aggcattttt gcccccaacc ctgtctacat cattcatgcc gagttggtta taccaaagca
     2461 ccagcggtga cgagccattg ttggacatcg aacaatcctt ttgtgataaa tgaacggttt
     2521 gagaaacaca tttctgcgca tttatctttg catatttaac cggatgaaaa aaacggtgaa
     2581 tcgttcaagc aaatatattt ttttactttt taagactgat cccagcgttg cgcaaatctg
     2641 ctctcttcga caatttccac ataaaccatc ggtataaacg aaaccataaa aatgaaatgc
     2701 tgttttcata aaaaataaaa ttgaaggttc attttataaa ccagacataa cgtcctacgc
     2761 ttctgtgcgt ttttaattta tgctttcata gaattatgtc tgcatcacgg gaagaacaaa
     2821 atggataatg ctgttgatcg ccacgttttt tatatttctg atggtacggc aataactgcg
     2881 gaggtattag gacacgcagt aatgtcacaa tttcccgtca ctatcagcag cattacgctg
     2941 ccgtttgtcg aaaatgagag ccgtgcacgg gcagtgaagg atcagattga cgcaatttat
     3001 caccagacag gtgtgcgccc gctggtcttc tactccatcg tgttgccgga gattcgcgcc
     3061 atcatcttgc aaagtgaagg cttttgccag gatatcgttc aggcgctggt tgccccgcta
     3121 caacaagaga tgaaactgga tccaacgccg attgctcatc gtacccatgg ccttaaccct
     3181 aataatctca ataaatatga tgcgcgcatt gcggcgattg attacaccct cgcccacgat
     3241 gacggcattt cgttgcgcaa tctggatcag gctcaggtga tcctgctcgg tgtttctcgc
     3301 tgtggtaaaa cccccaccag tctttatctg gcaatgcaat ttggtatccg cgcggcaaac
     3361 taccccttta ttgccgacga tatggataat ctggtgctac ccgcgtcgct caaaccgctt
     3421 cagcataaat tgttcggcct gactatcgac ccggaacgtc tggcggcgat tcgcgaggaa
     3481 cgtcgggaga acagtcgcta tgcctcgctt cgtcagtgca ggatggaagt cgcggaagtg
     3541 gaagcgttgt accgtaaaaa tcagatcccg tggattaaca gtaccaatta ttcggtagaa
     3601 gagattgcca ccaagatcct cgatatcatg ggccttagtc gccgaatgta ctagagaact
     3661 agtgcattag cttatttttt tgttatgatg ctaaccaccc ggcgaggtgt gacacacctc
     3721 gcacttgaaa tcagcagcga ttggtttatc gtgatgcgca tcacttcccg gcagtcctgc
     3781 cgtagaagca acaaatttct gagacttgta atgaacagaa ctgacgaact ccgtactgcg
     3841 cgtattgaga gcctggtaac gcccgccgaa ctcgcgctac ggtatcccgt aacgcctggc
     3901 gccgccaccc atgtcaccga ctcccgccgc agaattgaaa aaatactcaa tggtgaagat
     3961 aagcgactgt tggtcattat tggcccctgc tcgatccacg atctcaccgc tgcaatggag
     4021 tacgcccccc gtctgcagtc gctgcgcaac cagtaccagt cacggctgga aatcgtaatg
     4081 cgcacctatt ttgaaaaacc acgaactgtt gtcggctgga aaggactaat ctccgatcca
     4141 gatttaaacg gcagctatcg ggtaaatcac ggtctggagc tggcgcgcaa attactttta
     4201 caggtaaatg agctgggcgt cccaaccgcg actgagttcc tcgatatggt gaccggtcag
     4261 tttattgctg atttaatcag ttggggcgcg attggcgcac gtactaccga aagtcagatc
     4321 caccgtgaaa tggcttcggc actctcctgt ccggtaggtt ttaaaaatgg taccgatggc
     4381 aatacgcgga ttgctgtgga tgctatccgc gcagcccgcg ccagccatat gttcctctcg
     4441 ccagacaaaa atggtcagat gaccatctat cagaccagcg gcaacccgta tggccacatt
     4501 attatgcgtg gcggcaaaaa accgaattat catgccgatg atatcgccgc agcctgcgat
     4561 acgctgcacg agtttgattt acctgaacat ctggtggtgg atttcagcca cggtaactgc
     4621 cagaagcagc accgtcgcca gttagaagtt tgtgaggata tttgtcagca aatccgcaat
     4681 ggctctacgg cgattgctgg aattatggcg gaaagtttcc tgctcgaagg aacgcaaaaa
     4741 atcgtcggca gtcagccgct cacttacggt caatccatta ccgacccgtg tctgggctgg
     4801 gaagataccg aacgcctggt cgaaaaactc gcctctgcgg tagatacccg cttctgaatg
     4861 cgtgcccatt cctgacggaa tgggcatttc tgcgcaactt gttgtcttct caacaaatta
     4921 ctgcttgctc tggcctgcca taatattgat aataagaatc attgttatat caattattat
     4981 taatctttat gcgttatacg gatagcagaa aactcacgcc tgaaacggac gccaatcaca
     5041 aggccgcttc cccgcagccc atccggcgaa ttaccagcca gacactgtta ggtccggatg
     5101 gcaaactgat tatcgatcat gacgggcaag aatatctgct ccgtaaaact caggctggca
     5161 agctgctgtt aactaagtag cctttaactc gagcagctga cttccagccg tttaccccag
     5221 tcaggtggac gactgacata gtcatcatct ctatcgctga aaggatttcg caacgcccca
     5281 tgcaggcggt gcaattccgt catatcaccc ttttctgccg cttcaatcgc ccgttgcgcc
     5341 aaccagttgc gcaataccag agcggggtta acgctttgca tcaactgctg acgctcacta
     5401 tcgctaacct cgtcttgttg caaacgcccc cgataacggg caaaccagcc atcaaatgcc
     5461 gcacgatcaa taaactcatc acgtagcggt gacgccgtgc tgtgctgctc ggtcagactc
     5521 agcatgcgga atgtgcgggt ataatcgctg cgctctcgcg ccatcagact gaataattca
     5581 ttcagtagcg cgttatcctc tttttgctcc gtgatgaagc ccagtttctg ccgcatccgt
     5641 tgtccataat gcgtcaacaa aacctgctga tagctgtcca gggcctcatt cagggcatct
     5701 acggcaacaa atggtgacaa tgtctgcgcc agacgctgta aattccacaa cgcgacagca
     5761 ggttgattat caaaactata acgcccttga tgatccgagt gattacaaat aaaaccgggt
     5821 tcgtaatcat caagaaaacc aaacggcccg taatcaagcg tcagtcccag cagcgacatg
     5881 ttgtcggtat tcatcacccc atgtgcaaag ccgaccgtct gccattgggc aattaacgac
     5941 gcggtacgtg cgacaacatc gctaaaccag agacggtatt tgtcctcatc atcttcaaga
     6001 tgtgaccagt aatgacgaat agcaaagtca gctaactgac gaaccttttc cgactcgcgg
     6061 cggtagtaaa aatgttcgaa atgaccaaag cgcagatgac ttggtgccac acgcatcagc
     6121 atcgcgcctg gctccgccgt ttcccgatac actggcgaat cgctggtgac gatacttaac
     6181 gcgcgggtcg tcggaatgcc cagataatgc atcgcctcac tggcgagact ttctcgtatc
     6241 gtcgaacgta aaaccgcccg tccatcaccc attcgcgaat aaggcgtcag gccagcacct
     6301 ttcagatgcc agtccattgt agtgccatca gcaagcagtt gttcgccgag taaaatgccg
     6361 cgcccatcac ccagttggcc cgcccagacg ccgaactgat gaccactgta aacctgcgcc
     6421 agtggtgaca taccaggcag tagggcttcg ccgccccaga cacctgcgcc atttttaaac
     6481 agcgacgatg gaatactcag cgtgttagcc agttcggtat tatgccaaat cagccgggca
     6541 ttatttaaag gcgtagggga aagtgctgta taggtttctg gcaattcatc gcgccagcgg
     6601 gtaacaaaag acagggtcat agatcctcct gtttgatagt gtagacggtt actctcgtct
     6661 taaacaccag caaacagaag ggttatcgct gaaccaacgt cgttatctga tttatgggaa
     6721 cggcaggcca caggcatccc tggagagcat ggaaatcgaa gggggtaatc tgtgccaata
     6781 tttctgccgt atcaatgcct cccgcaatga tgcaattgca acaaggggaa atttgcgcct
     6841 ggatcgcacg aataaacggt tcgaaggagc ggtgcgtgat ttgttgctga ataaaacttt
     6901 tatccagcat gacgcgggtg aaaagaccat caaaaaccgc tttcattgtg ctattgcctg
     6961 ctcccaaatt acctaacaca aggggataca cttgcgataa ggataaaaga ccgcgattat
     7021 cttttccttc attgagatgc gggtaattct cattaatcaa cagttcaata aacggatatt
     7081 ttaatagttc gcctgcgtaa ttatccctct ctaataataa tgtagccact tgtggcgtta
     7141 aattaagcca ggcaaagagt ttatgttgaa taaaaaaatg ttggcatgat tttagtaatt
     7201 ccaactgctc tgaaaataat tgccaatgtt gctcctcggt aagttgcgct ataacccgac
     7261 tcgtcgggat acgcaccgtg ccatcttcac tggagaaatg ggtaattaac tcaacgccga
     7321 caaggacttg ctggttatct cttatcggca ggaaatagca atcagaatga taaagattct
     7381 ccaaaaaaat cttcatggta accgtccctc tcgaaggatg tttttcagta tccggctaaa
     7441 agggatgagg ccataagaca taacaattac agaaggagta actttcattt gttccatgtt
     7501 aaccactttt tcaggggtcc tttttttaga ttatcctgat tataaacgaa taatccattt
     7561 acggaatttt tgtctgcaaa atactactgt attcaggagt aaatggacgg cgatgtatca
     7621 tcgccgtcca tttttcgaca
//
LOCUS       DAJTYZ010000169         7603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000169 DAJTYZ010000000
VERSION     DAJTYZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.169"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(142..477)
                     /locus_tag="OO596_003665"
     CDS             complement(142..477)
                     /locus_tag="OO596_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6719426.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(481..825)
                     /locus_tag="OO596_003666"
     CDS             complement(481..825)
                     /locus_tag="OO596_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6719427.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(827..1000)
                     /locus_tag="OO596_003667"
     CDS             complement(827..1000)
                     /locus_tag="OO596_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719428.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1101..1286
                     /locus_tag="OO596_003668"
     CDS             1101..1286
                     /locus_tag="OO596_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719429.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1247..1366
                     /locus_tag="OO596_003669"
     CDS             1247..1366
                     /locus_tag="OO596_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6719430.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1845..2111)
                     /gene="minE"
                     /locus_tag="OO596_003670"
     CDS             complement(1845..2111)
                     /gene="minE"
                     /locus_tag="OO596_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6719431.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2115..2927)
                     /gene="minD"
                     /locus_tag="OO596_003671"
     CDS             complement(2115..2927)
                     /gene="minD"
                     /locus_tag="OO596_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6719432.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2951..3646)
                     /gene="minC"
                     /locus_tag="OO596_003672"
     CDS             complement(2951..3646)
                     /gene="minC"
                     /locus_tag="OO596_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6719433.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4166..4534
                     /locus_tag="OO596_003673"
     CDS             4166..4534
                     /locus_tag="OO596_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR6719434.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4642..5043)
                     /locus_tag="OO596_003674"
     CDS             complement(4642..5043)
                     /locus_tag="OO596_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR6719435.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5285..5578
                     /locus_tag="OO596_003675"
     CDS             5285..5578
                     /locus_tag="OO596_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6719436.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5650..6309
                     /locus_tag="OO596_003676"
     CDS             5650..6309
                     /locus_tag="OO596_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6719437.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6401..6847
                     /locus_tag="OO596_003677"
     CDS             6401..6847
                     /locus_tag="OO596_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6719438.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7054..>7603)
                     /gene="hlyE"
                     /locus_tag="OO596_003678"
     CDS             complement(7054..>7603)
                     /gene="hlyE"
                     /locus_tag="OO596_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCR6719439.1"
                     /translation="DGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSY
                     FQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFFT
                     TLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKM
                     INTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1954 a         1705 c         1767 g         2177 t
ORIGIN      
        1 gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac accgcagtga
       61 tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt cgaattcatc
      121 gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt catcaattgc
      181 cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt ctaccacttt
      241 ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc tggcattacc
      301 catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt cgttggcttc
      361 tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt cctgcgccca
      421 ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca tcatcattcc
      481 ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac ctttaatcgc
      541 cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg cacccacttt
      601 tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac caacaccagc
      661 gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta atccaacgcc
      721 tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg gcgtggagca
      781 gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag atgtccggta
      841 ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta actaaagaac
      901 gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga attatgtttt
      961 ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa tatttttatt
     1021 caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct gatttatcaa
     1081 tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt acattagtca
     1141 ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat tatctgaaaa
     1201 gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc ctctttctta
     1261 tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca atacacctgc
     1321 gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt gcagcagagc
     1381 ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa tcagtcgata
     1441 cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact gcgcttcggc
     1501 gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa gcccgccagt
     1561 tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg gggttatcat
     1621 cccccagtgt atccacacta acaataaggt tatttactca gccaggcata aacattatgt
     1681 tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca atgaggagta
     1741 tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa aactcaaggc
     1801 agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt cagctcttct
     1861 gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt ttgctcaagc
     1921 tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc aagaatatct
     1981 ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc agcaacaata
     2041 atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag aaagaaatcg
     2101 agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt ctcttcttca
     2161 atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc gtaggcttta
     2221 cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc gcgtaatact
     2281 gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag cacatcttcc
     2341 atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa cagcaggtgc
     2401 tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag aatgcctaaa
     2461 atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat ggcttcgtct
     2521 gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc acaaacgata
     2581 aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc acgggtgagg
     2641 gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt acgcttatct
     2701 ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa atcgtaaacg
     2761 acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc aaaatctatc
     2821 acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct ggaggttgtc
     2881 ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga aattccttgt
     2941 taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac tgcagtcgcg
     3001 ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca cctgcgatgg
     3061 acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca ccacttgccc
     3121 ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg gcaatcaatt
     3181 cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca taaatacgct
     3241 gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg gtattttgcg
     3301 ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc gtcaggatag
     3361 gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca cttacgccaa
     3421 taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt accgggtctt
     3481 ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg gcctgagcga
     3541 ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga tgaaccacag
     3601 ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg gccttactca
     3661 attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag gcatgttata
     3721 gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag tctattctgt
     3781 gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc attgccgccg
     3841 tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg acagcatctc
     3901 cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg ccaggctttc
     3961 ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct taatatccgc
     4021 cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt caggtcatcg
     4081 taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc ataagtgttg
     4141 tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata tcggattgtc
     4201 aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg gacttcaatc
     4261 tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg gcatttcccc
     4321 agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat cattacaagg
     4381 ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta aagaaaaatt
     4441 atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa tcaatcaaac
     4501 caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta tgttctctgc
     4561 tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc tatgatgggg
     4621 atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt ttttagtttt
     4681 gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc cagaagcagg
     4741 gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat caacagaatc
     4801 atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac tcacatgcac
     4861 tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct ttatttcgcc
     4921 ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt tacccgcagc
     4981 aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc tcttgatttt
     5041 cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa caatccctta
     5101 cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca ttttttgccg
     5161 gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac agctggttgg
     5221 cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct taaagagtaa
     5281 aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt tatatgtcga
     5341 aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg gtcagcctca
     5401 gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg atattgaaaa
     5461 ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac cacctgaaga
     5521 tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta acaaataacc
     5581 gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg ataaggtgag
     5641 gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta tccggtgagt
     5701 aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg cagcgcagtg
     5761 cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct gcggcagcca
     5821 ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc ggtgttgatt
     5881 ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc cggttacggc
     5941 gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccg
     6001 tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat ctccgcggcg
     6061 gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg cgaacaacgc
     6121 cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc ttatatgagc
     6181 aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga tggcgtcggc
     6241 ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt gacaactcgc
     6301 gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc tgtgccagac
     6361 tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg tacctttctg
     6421 gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg
     6481 cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct acttcactaa
     6541 cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg aacgtcgttt
     6601 cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct
     6661 gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg cgtggcatcc
     6721 gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc gtcatatcgc
     6781 agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac ctgactgggc
     6841 gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct ggatgctcgc
     6901 catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc gataccttat
     6961 tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt gataaacctt
     7021 ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct caaagagtgt
     7081 ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt tggccgcttc
     7141 ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg tttcagtttc
     7201 cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg cggcatcgat
     7261 atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct gcacagattt
     7321 taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag caatagaata
     7381 ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg catacgcttc
     7441 cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa aatcattggt
     7501 taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt gtgagcttac
     7561 cagcagggat ttttgcgctt cattcagctt cgtgatgccg tca
//
LOCUS       DAJTYZ010000170         7508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000170 DAJTYZ010000000
VERSION     DAJTYZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.170"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1780)
                     /locus_tag="OO596_003679"
     CDS             complement(<1..1780)
                     /locus_tag="OO596_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6719440.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="OO596_003680"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="OO596_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6719441.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="OO596_003681"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="OO596_003681"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6719442.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="OO596_003682"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="OO596_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6719443.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="OO596_003683"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="OO596_003683"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6719444.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="OO596_003684"
     CDS             complement(5968..7005)
                     /locus_tag="OO596_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6719445.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7301..>7508
                     /locus_tag="OO596_003685"
     CDS             7301..>7508
                     /locus_tag="OO596_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6719446.1"
                     /translation="MLKGTFTPKRTKEERLADRPGIKQLVACIDGDVVGHLTIDVQQH
                     PRRSHVADFGICVDSRWKNRGVASA"
BASE COUNT         1896 a         2045 c         1800 g         1767 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggagg agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc
     7381 ctgtattgat ggagacgtcg tgggccatct caccattgac gtgcaacagc acccacgccg
     7441 cagtcatgtt gccgattttg gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc
     7501 cagcgccc
//
LOCUS       DAJTYZ010000171         7491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000171 DAJTYZ010000000
VERSION     DAJTYZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.171"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="OO596_003686"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="OO596_003686"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6719447.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="OO596_003687"
     tRNA            647..722
                     /locus_tag="OO596_003687"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OO596_003688"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OO596_003688"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR6719448.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="OO596_003689"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="OO596_003689"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6719449.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OO596_003690"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OO596_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR6719450.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="OO596_003691"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="OO596_003691"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6719451.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="OO596_003692"
     CDS             complement(5917..6249)
                     /locus_tag="OO596_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR6719452.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="OO596_003693"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="OO596_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6719453.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1825 a         2029 c         1868 g         1769 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAJTYZ010000172         7466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000172 DAJTYZ010000000
VERSION     DAJTYZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.172"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..323)
                     /locus_tag="OO596_003694"
     CDS             complement(<1..323)
                     /locus_tag="OO596_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6719454.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRI
                     GLRQN"
     gene            complement(1144..1356)
                     /locus_tag="OO596_003695"
     CDS             complement(1144..1356)
                     /locus_tag="OO596_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HCR6719455.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(1432..2049)
                     /locus_tag="OO596_003696"
     CDS             complement(1432..2049)
                     /locus_tag="OO596_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCR6719456.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2316..3815
                     /gene="ansP"
                     /locus_tag="OO596_003697"
     CDS             2316..3815
                     /gene="ansP"
                     /locus_tag="OO596_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR6719457.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3930..4991)
                     /locus_tag="OO596_003698"
     CDS             complement(3930..4991)
                     /locus_tag="OO596_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR6719458.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5233..7335
                     /locus_tag="OO596_003699"
     CDS             5233..7335
                     /locus_tag="OO596_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6719459.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7371..>7466)
                     /locus_tag="OO596_003700"
     CDS             complement(7371..>7466)
                     /locus_tag="OO596_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6719460.1"
                     /translation="ALKAKDKEGCRHCLAEIMQQNIAILYQQYNR"
BASE COUNT         1904 a         1746 c         1804 g         2012 t
ORIGIN      
        1 aagttctggc gcagccctat gcgccacagc ggtgggcata ccttcatgct gtacagcatc
       61 tggtttttgt cgttctcccc cagttcaaac caggtcacca cacctttcac ccggcgctgt
      121 acgtcatcgc cctgccatac cgtcaggtag gccattttgt ccagcacctg cgcaaattca
      181 agggagagaa actgctggct gaccagagag agatcgaggg aaaagagcga agagagtgac
      241 tggttcaaat gaaaggaaac caccgcaaaa gcatccggtg gcaggccgtc cacttccagt
      301 gtgaaacgta atccggttga catagcaacc tccgtgaaaa tgaataataa tccgcacata
      361 ttgcatgtac aacatgtcgg gcggatgaga taagcgagtc gacatctggc gttaccgggg
      421 aatatcagaa aaatgtcatg gttatgtgat gatttagtct gggattttac ttcagagaaa
      481 cgggtgtctg tttagtcaac aaaataatca caaacgcaac ggatgggttc tcgacgttat
      541 tcacgacagg ctcgtacatt acagcgagta ataaaaaacc tgatatgcaa agtcaaacca
      601 atattcataa tgtcttttta ttcacaacaa taaataattc aataacaatc attgaaatga
      661 atgatggttt gtataaggaa tatacgctac cgcccaccct gataacatga tctgcacatt
      721 gccatcttaa cgtcgttaaa cactggctta atagtggata tttcaaatta ggaatttatt
      781 aaattcacct gatgaatata ataagcaaat agcatagaaa ataatggcaa ctccccccga
      841 gaggaccatt tccaaagcac tgcaccacca cctggtgccc ggatgatctt gcttcagtgg
      901 taagagtacc tgattcattg ttcccaaaat gatgttttcc ccggccccgc caataatgtg
      961 cagctctcac gtgataatct attgattagg ttatattaag aatcaatatt taaaatacat
     1021 ccattttctt gaataaatca acatatttat ttatcaattg ctgttttagc tctgactcat
     1081 caagctctcc atatttatcc tatagataat tgcgaataaa tacagagaac gccatttatc
     1141 cttttagcat acctgccgga tatttttatt tgcattaata ttaattgcat ctccaactcc
     1201 gagcatatcc atcattaaaa actttttgag attatagaat acctgtgata acaagtgaaa
     1261 catctgcaca atgtgaatag ttagtttctt ttcagaggaa atgaaataga caagctgact
     1321 gaaggggaga gtgatataaa tcaaaaaaca taacatgaat ttaattaatt acgcccatca
     1381 ttgtggataa agttactgaa taataaataa cataatcctc tggtatccca ctcaaataat
     1441 ttcattcctt tttaatactt cttgtaattt cggtaactga catacagcat cagcaatcgc
     1501 actgatattc gtggcattat cctgaaacca ttcatgccca gacccccatg tgcgcatagt
     1561 gcaaagataa cagtccacca gcgttagttg ctcaccaaac gcataaggtt cagcggtcaa
     1621 ttgtgaattc agccagatat aaagcgattt acgatattca ataacattct tctttaactg
     1681 ttcaggtgcg tcaggagccc aacgctcagg gtaatcggcg aaagtgaatg ttggatagac
     1741 attagcaacc agccagacca atagccgttg aaactgctga cgttcggcgc gcccaaccgg
     1801 tggcgcaagg tccgggcgac gatcgagaac catcaatgca atcgccgctg tctccgtcat
     1861 aatctcgtca ttttccagcg ccagtgtcgg aatctgacac aacgggttca gggttttcaa
     1921 taactcgcgt gaagttccct cgtggtcaaa accactgaca tcaacgaact gataaggaat
     1981 atcagccagt gttagcatca gctcactgat tgtagagccc catccgggta cgccatacac
     2041 cttaatcata tgcccctcac aatcttttaa agattaagtg taggacagta cacaggttaa
     2101 ccagcaaaag tatttgtcat tttttcgtca gttgtatgaa tccccttcag gattgtaagg
     2161 ggatgttttg catcgtatat cgataacggg taggatgctg cgccgatagt tttataccca
     2221 taaagaataa tggtgataac tatcatcgcc aggatgaata aacattgttc atggcaactt
     2281 atatgacttt ttcattaaag caatcaggga gagcaatgag taaacacgac accgacactt
     2341 cagatcaaca cgccgcgaaa cgccgctggc ttaatgccca cgaagagggg tatcacaaag
     2401 cgatgggcaa tcgccaggta cagatgatcg ccattggcgg cgcgattggc accggcttgt
     2461 ttttaggtgc aggagcccga ctgcaaatgg cgggtccagc actggcactg gtttatttaa
     2521 tttgtggctt gttttcgttt tttattctgc gtgcattggg tgagctggtg ctacaccgcc
     2581 cttccagtgg cagttttgtc tcttatgccc gtgagttttt gggtgagaaa gccgcttatg
     2641 ttgctggctg gatgtacttc atcaactggg cgatgacggg gattgttgat attaccgccg
     2701 tcgctctgta tatgcattac tggggtgcgt ttggcggcgt gccgcagtgg gtctttgcgc
     2761 tcgctgcact taccatcgtt ggcaccatga atatgatcgg tgtgaaatgg tttgcggaga
     2821 tggagttctg gtttgcgctt attaaagtgc tcgccattgt gacctttttg gtcgtgggta
     2881 cagtgttcct cggtagtggt cagccgctgg atggcaacac cactggcttt catttaatca
     2941 ccgataatgg cggcttcttc ccccacggtt tgctgcctgc gctggtgttg attcagggcg
     3001 tagtgtttgc ttttgcctcc attgaaatgg tgggtacagc tgccggagaa tgtaaagatc
     3061 cgcagaccat ggtgcctaaa gccattaaca gtgtgatttg gcgtattggc ctgttttacg
     3121 tcggctccgt ggtgttgctg gttatgttat tgccgtggag cgcgtatcag gcggggcaaa
     3181 gtccgttcgt gacgtttttc tctaaactgg gtgtgccata tatcggcagc attatgaaca
     3241 ttgtggtgct gaccgctgcc ctctccagcc tgaactcagg tctgtactgc accgggcgta
     3301 ttctgcgctc gatggcgatg ggcggttccg caccgagttt tatggcgaaa atgagtcgtc
     3361 agcatgtgcc gtatgccggg attctggcga cactggttgt gtatgtcgtc ggcgtattcc
     3421 tcaactatct ggtgccgtcg cgcgtatttg agattgtgtt gaacttcgcg tcgctgggaa
     3481 tcatcgcttc atgggcgttt atcatcgtgt gccagatgcg cctgcgtaaa gcgattaaag
     3541 aaggcaaagc agcggatgtc agttttaaac tgcctggcgc gcccttcact tcctggctga
     3601 cattactgtt tttactgagt gtccttgtgc tgatggcgtt cgattacccg aacgggactt
     3661 atactatcgc ggcgctgccg attatcggta ttctgctggt tataggctgg tttggtgtgc
     3721 gcaaacgcgt tgctgaaatt cacagcactg cgccagtcgt cgaggaagat gaagaaaaac
     3781 aggaaattgt gtttaagcct gaaacggcga gctaattgca aaattgcccg gctacgtgcc
     3841 gggcaaattt agattactga cgaaggtgtg ccgcatgcgg cgtgataaaa gtcaggcaag
     3901 tctctccgtt ttcaccctcg ccatatctat tacagcgcaa tacgaatcac atcgtctggc
     3961 tgggtagctt cctgctgttt agtggatttt tgtttcacac tgacatacag cgttttgcca
     4021 tcggcagaca gcgccaggct gtttggatga gtcggcgtat cgaacgtttt cactacttta
     4081 tagcttttcg cgtcaatcac actgacttta cctgcctgac gatgcgttac gtaggcttca
     4141 ttacgcgctg ggttaaacag cacagccagt gattccggtg ctgcaacctt cgccagaata
     4201 ttgccattac gggtatcgac cactaacact tctgcggctt tagaatcggt gataaatgca
     4261 cgctgcctag cggtatcaag gctaatgttg ataaagaagt gctctttgcc gtcatccagc
     4321 agctttttac ggctgaggat tttattgtcg gcggtgtcga tggtaatcaa ttcgccgtca
     4381 gcgttagtgg tgtaaagacg tttgcctttg ctatcaagcg ccagaccggt actcatttta
     4441 ccggtgttct ggatggcggt tttcagtttg atattctcgc catcaacgac ccaaatcacg
     4501 ctctctttac caataccact gatgtaaacg gtgttcgtgg catcgtcagc taccagctca
     4561 cgcggttgca gcgggcgcac ctcttccgta cgcttacgat catccagcac cagacggcct
     4621 ttcacctcgc ccgttttggc atctatcgcc gtgaccgcgc tgtttacagt gttaccaaac
     4681 cacagtgtct gagtcgtgtt attgatggtg acaccaaacg gcttgagatc gttatggatc
     4741 gcctgcgtca cttccagagt gaccggatca agacgataaa ccacgccgcc tttatccagt
     4801 ttgcggcttt gcgaagtggc gagccacagc gcgttttctt gctggctata agccatttcg
     4861 taggcacctt tacctaccgc tttacgcagc atttcttctg cggcctgcgt actgaatgat
     4921 gaagcaacaa gcaatgaacc taacagtaat gaaccacgca ggcgcgatga aaacagatga
     4981 cgtaaatgca tgacgactcc ctttgatgaa ccgatatttt tgctgcttca caatgcttcg
     5041 ctaagcgtaa tcatcgttac gctcttgtta taaatgggaa tggtaatcat tattttctca
     5101 aatgtggagc atttcttgcc gttacgacta caattttctc aattacttaa cgtatcgtgc
     5161 cgtttcgttt tccatgaatt tacaatttta ttaccataac ggcgttttcc cattcttaac
     5221 ctggcttttt tcatgaagat tttttccgtc cgacagaccg ttttgcccgc actacttgcc
     5281 ctttcccccg ttgtttttgc cgctgatgaa cagaccatga ttgtcagtgc cgcaccgcag
     5341 gtggtttcag aactggatac cccagcagca gtaagcgtgg tggatggcga ggagatgcgc
     5401 ctggcaacac cgcgcattaa cttgtccgaa tcactgaccg acgtgcctgg tttgcaggta
     5461 caaaaccggc agaactatgc gcaagattta cagctgtcga ttcgcggatt tggctcccgc
     5521 tccacttacg gtattcgcgg tattcgcctg tatgtggacg gtattcccgc caccatgccc
     5581 gacgggcaag ggcaaacatc caacatcgat ttaagcagtg tgcaaaatgt ggaagtgctg
     5641 cgtggcccct tctctgccct gtatggcaac gcgtctggtg gggtaatgaa tgtcaccacc
     5701 cagaccggac aacagccacc aaccattgaa gccagtagtt actacggcag ttttggcagc
     5761 tggcgctatg ggctgaaagc aacgggcgca acgggagacg gcacacagcc tggcgatgtc
     5821 gattacaccg tctcaaccac gcgttttacg acccacggct atcgtgacca tagtggcgca
     5881 cagaaaaatt tagccaatgc caaactgggc gtacgcattg atgaagccag caaattaagt
     5941 ctgattttca atagtgtgga tatcaaagca gatgacccgg gtgggctaac caaagcagaa
     6001 tggaaggcta atccacaaca agcgcctcgt gcagaacagt acgacacgcg aaaaaccatc
     6061 aagcaaactc aggctgggtt gcgctatgag cgtagcctga gcgcgcaaga tgatatgagt
     6121 gtgatgatgt atgccggaga gcgagaaacg acccagtacc agtcaatacc catggcacca
     6181 caacttaacc cgtcacatgc gggcggcgtg attaccctgc aacgccatta ccaggggata
     6241 gacagccgct ggacacaccg tggtgaactg ggcgttccgg tcacgttcac taccggcctg
     6301 aactacgaaa acatgagtga aaaccgcaag ggctacaata acttccgcct gaatagcggc
     6361 atgccggagt acgggcaaaa aggtgagttg cgtcgcgacg aacgcaatct gatgtggaac
     6421 atcgatccct atttacagac gcagtggcag ctgagcgaaa aactgtcgct ggatgctggc
     6481 gtgcgctaca gctccgtgtg gtttgattcc aacgaccatt acgttactcc gggtaacggc
     6541 gatgacagcg gtgatgccag ttatcataaa tggctacctg ccggttcgtt aaaatatgca
     6601 atgaccgatg cctggaatat ctatctggca gccgggcgag gttttgaaac gccgacgatt
     6661 aatgagctgt cttatcgtgc tgatgggcaa agcggtatga actttggttt aaaaccatcc
     6721 accaacgata caattgagat cggcagtaaa acgcgtattg gtgatgggct gcttagtctc
     6781 gcattgtttc agaccgacac tgatgatgaa attgttgtcg atagcagtag cggtgggcgt
     6841 acgacttaca aaaatgccgg aaagacccgt cgtcaaggcg ctgaactggc atgggatcaa
     6901 cgtttcgcag gagattttcg cgtaaacgcg tcctggacct ggcttgatgc gacctatcgc
     6961 agcaatgttt gcaatgaaca ggattgtaac ggtaatcgga tgccagggat cgcccgtaat
     7021 atgggctttg cgtcgatagg ttatgtaccg gaagatggtt ggtatgcagg cacggaagcg
     7081 cgttatatgg gcgatattat ggcagatgat gaaaatacgg caaaagcgcc gtcttatact
     7141 ctcgtcggct tattcaccgg gtataaatac aattaccaca atttaactgt ggatttattt
     7201 ggtcgtgtcg ataatttatt cgataaagaa tacgttggtt ctgtcattgt caatgagtca
     7261 aacgggcgat attacgaacc ttcgcccgga cgaaattatg gtgtcggcat gaatattgcg
     7321 tggagatttg agtaattacc ttgatgcccg gtatttgccg ggcatttact ttaacgattg
     7381 tattgctggt ataaaatagc aatattttgt tgcataattt cagcaagaca atgtctgcat
     7441 ccctctttgt cttttgcttt taacgc
//
LOCUS       DAJTYZ010000173         7441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000173 DAJTYZ010000000
VERSION     DAJTYZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.173"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(99..1607)
                     /gene="putP"
                     /locus_tag="OO596_003701"
     CDS             complement(99..1607)
                     /gene="putP"
                     /locus_tag="OO596_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR6719461.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2029..5991
                     /gene="putA"
                     /locus_tag="OO596_003702"
     CDS             2029..5991
                     /gene="putA"
                     /locus_tag="OO596_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6719462.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDNEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6031..6669)
                     /gene="rutR"
                     /locus_tag="OO596_003703"
     CDS             complement(6031..6669)
                     /gene="rutR"
                     /locus_tag="OO596_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6719463.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6957..>7441
                     /locus_tag="OO596_003704"
     CDS             6957..>7441
                     /locus_tag="OO596_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6719464.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1777 a         2065 c         1945 g         1654 t
ORIGIN      
        1 ctgcgcgctc cctgcaccat tcaatttata tggcgaggca cattaaggga gcgcaacccg
       61 ctgctgaagc caaacaaaac cgccgcaggc taagtccctt agttttcctg caaccgtgac
      121 ggcggagccg aatgatagtg cgcatcggcc tcggcaaagc gtttttgcat cgccgctgac
      181 ggcgctttac ccagcaaact aaacactaca atcccgatac tgccgaagat gaagcccgga
      241 ataatttcgt acagtcccag ccagccgaac tgcttccaga cgataactgt cagcgcaccg
      301 atgatcatcc ccgccagcgc accgttgcgc gtcatgcgtg accacatcac cgagaacagc
      361 accaccggac caaacgccgc gccaaagcct gcccacgcgt agctcactaa gcccagcacg
      421 cggttttccg ggtttgccgc cagcgcaatc gccaccagcg ccaccaccag caccatcaca
      481 cgtcctaccc acaccagctc tttctggctg gcctgtttac gcagaaacgc tttgtataaa
      541 tcttcggtaa tcgcactgga gcacaccagc agctggcaac ttaaggttga cattaccgcc
      601 gccagaatcg ccgacagcag aatcccggca atccacgggt taaacagaat ttgcgccagt
      661 tcgataaaca cgcgttcagc gttctggttt accgcacctg ctaccgccgg atgctcgtta
      721 aagtaagcga taccaaagaa gccaacagcc accgcccctg ccaggcaaag gatcatccag
      781 gtcatactga tacgacgcgc atggacaatg ctgtggtgag aatctgccgc cataaaacgc
      841 gccaggatgt gcggctgccc gaagtaaccc agcccccaac ccatcaatga gataatggca
      901 acaaagttca gccctttgag catatccacg ttttcgatgc tcttttgttt gatcacttcc
      961 agcgagtcac caaagccacc gacactgata atgacgataa ccggcgtcag gatcagggca
     1021 aaaatcatca ggctggcctg tacagtgtca gtccagctca ccgcgaggaa accgccaata
     1081 aaggtgtaaa ggatcgtcga cgcagctccc gccaacagag ccgtttcgta actcatgcca
     1141 aaggtacttt caaacagacg cgcgcctgcc acaatgcccg aagcgcaata aatggtgaag
     1201 aacagcaaaa taaccagcgc ggagataatg cgcaaaatgc ggcttttatc ttcaaaacgc
     1261 ccggtgaaat aatccggcaa tgttaaggcg ttattattgt attcggtatg cacacgcaac
     1321 cgcccggcca ccagcttcca gttgatccac gcgcctaatg tcaggccaat ggcgatccag
     1381 ctttcggaaa tcccggaaag aaaaaccgcg ccaggcaacc ccattaacag ccagccgctc
     1441 atatccgacg cacccgccga taatgccgtc acgaatggcc caagactacg accgcccaga
     1501 atatagtcgt caaagttttt cgttgatcgc caggcgataa acccaatcaa tatcatgcca
     1561 aagatataga cacaaaatgt caccaacatc ggtgtgctaa tagccatcta aagtctccaa
     1621 aaaattatta tcggcaatgt cgaaacttgc cgttatatct gccaccggaa cggggtaaca
     1681 gagtttatgt tttaccaggg cgaccgtatc ctgccggaag cgctggttat tcacaatcga
     1741 tttaacacac catttacatt aaattttagt gctcagcgac actatttttc atcaggttgc
     1801 actctctcac attttttgcg gttgcacctt tcaaaaaggt taactgccgc agagaaaaag
     1861 tctgagttat tttttaatca ctgtcatatc gatttctttt attaacattt cattcatttt
     1921 taagcttgct acgcaagtca catttaacat ggttgcacaa agttgcaaca tcatggatat
     1981 ttcacgataa cgttaagttg cacctttcag aacaacagga gtaatggcat gggaaccacc
     2041 accatggggg ttaagctgga cgacgcgacg cgtgagcgta ttaagtctgc cgcgacacgt
     2101 atcgatcgca caccacactg gttaattaag caggcgattt tttcttatct cgaacaactg
     2161 gaaaacagcg atactctgcc ggagctacct gcgctgcttt ctggcgcggc caatgagagc
     2221 gatgaagcac cgactccggc agaggaacca caccagccat tcctcgactt tgccgagcaa
     2281 atattgcccc agtcggtttc ccgcgccgcg atcaccgcgg cctatcgccg cccggaaacc
     2341 gaagcggttt ctatgctgct ggaacaagcc cgcctgccgc agccagttgc tgaacaggcg
     2401 cacaaactgg cgtatcaact ggccgataaa ctgcgtaatc aaaaaaatgc cagtggtcgt
     2461 gcaggaatgg tccaggggtt attgcaggag ttttcgctgt catcgcagga aggcgtggcg
     2521 ctgatgtgtc tggcagaagc gttgttgcgt attcccgaca aagccacccg cgacgcgtta
     2581 attcgcgaca aaatcagcaa cggtaactgg cagtcacaca ttggtcgtag cccgtcactg
     2641 tttgttaatg ccgctacctg ggggctgctg tttactggca aactggtttc cacccataac
     2701 gaagccagcc tctcccgctc gctgaaccgc attatcggta aaagcggtga accgctgatc
     2761 cgcaaaggtg tggatatggc gatgcgcctg atgggcgagc agttcgtcac gggtgaaacc
     2821 atcgcggaag cgttagccaa tgcccgcaag ctggaagaga aaggtttccg ttactcttac
     2881 gatatgctgg gcgaagctgc actgactgcc gcagacgcac aggcgtatat ggtttcctat
     2941 cagcaggcga ttcacgccat tggtaaagcg tctaacggtc gtggcatcta tgaaggtccg
     3001 ggcatttcaa tcaaactgtc ggcgctgcat ccgcgctaca gccgcgccca gtatgaccgg
     3061 gtaatggaag agctttaccc gcgtctgaaa tcactcaccc tgctggcgcg tcagtatgat
     3121 attggtatta acatcgatgc cgaagaggcc gatcgtctgg agatctccct cgatctgctg
     3181 gaaaatctct gtttcgagcc ggaactggca ggctggaacg gcatcggttt tgttattcag
     3241 gcttatcaaa aacgctgccc gttggtgatc gattacctgg ttgatctcgc cacccgcagc
     3301 cgtcgccgtc tgatgattcg cctagtgaaa ggcgcgtact gggatagtga aatcaagcgt
     3361 gcgcagatgg acggccttga aggctatccg gtttataccc gcaaggtgta taccgacgtt
     3421 tcttatctcg cctgtgcgaa aaagctgctg gcggtgccga atctaatcta cccgcagttc
     3481 gcgacgcaca acgcccatac gctggcagcg atttatcaac tggcaggtca gaactactat
     3541 ccgggtcagt acgagttcca gtgcctgcat ggtatgggcg agccactgta cgagcaggtc
     3601 accgggaaag ttgccgacgg taaacttaac cgaccgtgtc gtatttatgc cccggttggc
     3661 acacatgaaa cgctgctggc gtatctggtg cgtcgcctgc tggaaaacgg tgctaacacc
     3721 tcgtttgtta accgtattgc cgacacctct ttgccactgg atgaactggt cgccgatccg
     3781 gtcactgctg tagaaaaact ggcgcaacag gaagggcaaa ctggattacc gcatccgaaa
     3841 attcccctgc cgcgcgatct ttacggtcac gggcgcgaca actcggcagg gctggatctc
     3901 gctaacgaac accgtctggc ctcgctctcc tctgccctgc tcaatagtgc actgcaaaaa
     3961 tggcaggcct tgccaatgct ggaacaaccg gtagcggcag gtgagatgtc gcccgttatt
     4021 aaccctgcgg aaccgaaaga tattgtgggc tttgtgcgtg aagccacgcc acgtgaagta
     4081 gaacaggcgc tggaaagtgc ggttaataac gcgccaatct ggtttgccac gcctccggct
     4141 gaacgcgcgg cgattttgca tcgcgctgcc gtgctaatgg aaagccagat gcagcaactg
     4201 attggtattc tggtgcgtga agccggaaaa accttcagta acgccattgc cgaagtgcgc
     4261 gaagcggtcg attttctcca ctactacgcc ggacaggtgc gggatgattt cgctaacgaa
     4321 acccaccgtc cattagggcc tgtggtgtgt atcagtccgt ggaacttccc gctggctatt
     4381 ttcaccgggc agatcgctgc cgcactggcg gcaggtaaca gcgtgctggc aaaaccggca
     4441 gaacaaacgc cgctgattgc cgcgcaaggg atcgccattt tgctggaagc gggtgtaccc
     4501 cctggcgtgg tgcaattgct gccaggtcag ggggaaaccg tgggcgcgca actgacgggt
     4561 gatgatcgcg tgcgcggggt gatgtttacc ggttcaaccg aagtcgctac gttactgcag
     4621 cgcaatatcg ccagccgcct ggacgctcag ggtcgcccta ttccgctcat cgctgaaacc
     4681 ggcggcatga acgcgatgat tgtcgattct tcagcactga ccgaacaagt cgtcgtggat
     4741 gtgctggcct cggcgttcga cagtgcgggt cagcgttgtt cggcactgcg cgtgctgtgc
     4801 ctgcaagatg agattgccga ccacacgttg aaaatgctgc gcggtgcaat ggccgaatgc
     4861 cggatgggta atccgggtcg cctgaccacc gatatcggtc cggtgattga taacgaagcg
     4921 aaagccaata ttgagcgcca tattcagacc atgcgtagca aaggccgtcc ggtgttccag
     4981 gcggtgcggg aaaacagcga agatgcccgt gaatggcaaa gcggcacctt tgtcgccccg
     5041 acgctgatcg aactggatga ctttgccgaa ttgcacaaag aggtctttgg tccggtgctg
     5101 catgtggtgc gttacaaccg taaccagcta ccagagctga tcgagcagat taacgcttcc
     5161 ggctatggtc tgacgcttgg tgtccatacg cgtattgatg aaaccatcgc ccaggtcact
     5221 ggctcggccc atgttggtaa cctgtatgtt aaccgtaata tggtgggcgc agtggttggt
     5281 gtgcagccgt tcggcggcga agggttgtcc ggtaccgggc cgaaagcagg cggtccgctc
     5341 tatctctacc gtctgctggc gaatcgcccg gaaagtgcgc tggcagtgac gctcgcgcgt
     5401 caggatgcag agtatccggt cgatgcacag ttgaaagccg cattgactca gccgctaaat
     5461 gcactgcggg aatgggcagc aaatcgtcca gaattgcagg cgttatgtac gcaatatggc
     5521 gagctggcgc aggcaggaac acaacgattg ctgccggggc cgacgggtga acgcaacacc
     5581 tggacgctgc tgccgcgtga gcgcgtgttg tgtattgccg atgatgagca ggatgcgctg
     5641 actcagctcg ccgccgtgct ggcggtgggc agccaggtac tgtggccgga tgacgcgctg
     5701 catcgtcagc tagtaaaggc attgccatcg gcagtcagcg aacgtattca actggcgaaa
     5761 gcggaaaata taaccgctca accctttgat gcggtgatct tccacggtga ttcggatcag
     5821 cttcgcgcat tgtgtgaagc cgttgctgcg cgggatggcg caattgtttc ggtgcagggt
     5881 tttgcccgtg gcgaaagcaa tatccttctg gaacggctgt atatcgaacg ttcgctgagt
     5941 gtgaataccg ctgccgctgg cggtaacgcc agtttaatga cgatcggtta aacaggtagc
     6001 cggaggatgt tacaacctcc tccggcatct ttaacgtggt cgaatcccct caataataat
     6061 ccgctgcacg ttttcaaccg tttgattgaa aaatatctca tcgcgcaacg tcgcgcctgt
     6121 caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc aaatcatgaa
     6181 aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac cggcaatcag
     6241 cgcagatttc tcatcaatta atgccttcaa atcgcccgtc agttcatcca ttaacagcgg
     6301 cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat cgcgggagac
     6361 ttccagcttc agacggatgt actcttttat cgccgccagc ggagcgaaat cttcacgaaa
     6421 cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa tatacagcgc
     6481 ctcttttgac ggaaagtaat acagcagatt ggtttttgaa acacccgcca actctgcgat
     6541 ctgctccagc cttgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg ctgcgctaag
     6601 aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt tcactgcgcc
     6661 ttgcgtcatg cgctctcccc tcttgtagat tggcaacagg atagcaaaac ccccgcccgg
     6721 cctcgacctt ctgcactctc atcgcgcaca gatgcatgtt ttatgtgcaa ctgttttgac
     6781 cgtttagtcc actttttatc agatatttta gataacgtaa tattaacata ttaataacat
     6841 taacttttta aaaactggca tccgctttgc aaacaagcca atacgcggct gaaaaggata
     6901 tgcaggatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg ttgatgatga
     6961 aaattggcgt attcgtacct attggcaaca acggctggct catttcgacc cacgcgccgc
     7021 agtacatgcc gacctttgaa ctgaataaag ccattgtgca gaaagcggag cattaccatt
     7081 tcgatttcgc cctgtcgatg atcaaactgc gtggctttgg cggcaaaact gagttctggg
     7141 atcacaacct tgagtcgttc accttgatgg cgggtctggc agccgtgacc tcgcgcattc
     7201 agatttacgc cactgctgcc accttaacgt tacctccggc aatcgtcgcc cgtatggccg
     7261 caaccatcga ctccatctct ggcgggcgtt ttggcgtcaa cctcgtgact ggctggcaaa
     7321 agcccgtgta tgagcagatg ggtatctggc ctggcgatga ctatttctcc cgtcgttacg
     7381 actatctcac cgaatatgtt caggtgctgc gcgacctgtg gggcacgggg aaaagcgatt
     7441 t
//
LOCUS       DAJTYZ010000174         7430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000174 DAJTYZ010000000
VERSION     DAJTYZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.174"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..100)
                     /locus_tag="OO596_003705"
     CDS             complement(<1..100)
                     /locus_tag="OO596_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HCR6719465.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAEE"
     gene            complement(125..1225)
                     /locus_tag="OO596_003706"
     CDS             complement(125..1225)
                     /locus_tag="OO596_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6719466.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1613..2359)
                     /gene="cutC"
                     /locus_tag="OO596_003707"
     CDS             complement(1613..2359)
                     /gene="cutC"
                     /locus_tag="OO596_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6719467.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2373..2939)
                     /locus_tag="OO596_003708"
     CDS             complement(2373..2939)
                     /locus_tag="OO596_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6719468.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3155..4891
                     /gene="argS"
                     /locus_tag="OO596_003709"
     CDS             3155..4891
                     /gene="argS"
                     /locus_tag="OO596_003709"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6719469.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            5068..5430
                     /locus_tag="OO596_003710"
                     /pseudo
     CDS             5068..5430
                     /locus_tag="OO596_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5434..6504)
                     /locus_tag="OO596_003711"
                     /pseudo
     CDS             complement(5434..6504)
                     /locus_tag="OO596_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6497..>7430)
                     /gene="flhB"
                     /locus_tag="OO596_003712"
     CDS             complement(6497..>7430)
                     /gene="flhB"
                     /locus_tag="OO596_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCR6719470.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1921 a         1947 c         1775 g         1787 t
ORIGIN      
        1 tctcttctgc ccatgcgggg agcggtgctg ccgatgtcac cacctgcgct ccagcggcaa
       61 taccgctgtg tttaataaat tcacgtcttg ttaatgtcat aacttcctcc ctgatcaacg
      121 aggatcactg tttctcggta atatctttgg cgttgtactg gaaataccgc gttaaaatgt
      181 ccagttcgtt ttcgctcatg ctggttcgtg cccccattcc tttggcaatg gacggccacg
      241 cattgacggt gtaatggtcg gcggcaatag gggcatgaca accagcgcaa taggtatcgt
      301 caagtttttc agcgtattgc catagcggtt tacggtccgc taatccggga tcggtaagcg
      361 caccctgtaa agacgcctga cgccattgat tgccgtattc gtcagcctgc cattccccgt
      421 ttaccgtgag cgccttgata ccttcttcac ttaatgtggc tagcgccagc cgttgacctg
      481 ccgccaggta gagcgtgttt tcactgccct gcatttgata accctgcaac agaacgatcg
      541 gctgtttgcc actggcatca acgacggtga gatcggtacc aggattcacg gtagccagct
      601 cgcctatgcg agaagttttg aaaggataaa tatgtgcgcc attagtcact gaagtagcgg
      661 cctgactttc cagctcatgc gccgcgttgt catccatttt tatttctggc ggaaaatggg
      721 caatgccttt atgacaatcg atacaggttt cgccgccctt ttgtgctttg ttatgcattt
      781 tctgcgcaga ttcactttgc gaggcaatat ccatggcatc aaaagaatgg caactacggc
      841 acgttgcaga gtcagttgct tttaattctt tccatactgt ttcggccatt tcctggcgat
      901 gagtttcgaa cttatcgtca ctgtctattt tgccgctaac aaattcatga taaatatctt
      961 tagatgcttt taatttagca aataaataat ccatccctga ctttggaata tggcaatctg
     1021 cacattccgc acgtatccct ttctggttcg aaaagtggac agttccctga tattcctcaa
     1081 aagatttact catcgagtgg caggaaaggc aaaatgctgt atccgacgtt ttatgtaagg
     1141 ctttttgcgc cagcaataac ccgccgccac caaccacaac cgctatcagc aaaaacaata
     1201 acccaatgcg ttttttccct cgcatagatt cctctaaata aattccagaa caccggaatc
     1261 attgtataat taaatacaca acgaattaac cgcttgcata actcggcaca acactgcctg
     1321 aaataatcga taaagaatat gatttattac aatgtaatca ttaattgcta aggaataacc
     1381 cagttgctat ttagaatata tatttatcag ttttagcaat ttaaatccca taatcaatgt
     1441 gaatataaaa aatattatta gcacaccgct atgttcactg tcacccgctc aacagatcac
     1501 ggttatcgtt cgttttttat actgctcagg gataaaaaaa ggcccctgtt gaaattgcag
     1561 gggcctggta cgagcaagca tcatattggg cgacatgatg caacggtaaa aatcatttgg
     1621 cctgatggcg ttcaatgatt cctttcattt cagcaaccgc cgccccgtct acgatataac
     1681 gcgaatactc gtccgcgtgt tcatctgatg acatggacaa tccttgatta cgataacgca
     1741 tcggtgaggc ttgccacgct cccgcggagc tatggacttc cagcactccg gcatcgagga
     1801 agtggtgcag attttctgca cggactcctg ctccggccat aatgattgga gcatcacgat
     1861 gggcaataag ttccataatt tttgataaac cttgcagtgc gtctgatttt tgccctgatg
     1921 tcagtacccg ggcaatgccc aattccgcaa gattattgag tgtatataaa gggttagcgc
     1981 acatatcgaa ggcgcgatga aaagtcactg ccagcggacc ggcagcagcc attatttttt
     2041 ccattcgtgg catatcgaca ttcccgtcaa catcgagaac gcccgtcacc agtccaggaa
     2101 aacctaattc gcggaccgtg cgcacatcct caagaatggc ggcaaactca ccgtcgctgt
     2161 agcaaaaatc accaccgcgt gggcgaatta tcggatgcac agggatcgtc acccgctggc
     2221 gcacggattt cagtaccccc aacgacggcg ttaagccccc ctcttttggg gctgcgcata
     2281 attcaactct gtctgcgccg ttttgctgcg ccgttagtgc acattccatg ctgtaacagc
     2341 aaatttccag taatgccatt tttgctcctt aattacgctg actgctcgct ggcaacgatc
     2401 tcttcaatgg accacggatg aaacttaata gttgttttgc catcggtgac cgccagcgtc
     2461 ggattcggta atcgctcatg ctcacctttt ggtgaactgg ttttaactga gatccccggt
     2521 aggctgagtc cttcatcaga aagcaatgcc agcgcacggg cattcagcgt ttctggatca
     2581 cccagcagaa cgatttcaat atgttcccaa ccttcgtgtg ggtaacgttt ttccccgggc
     2641 cacggtagct ccacaataga aaactgccag tgcgcaacct gtaccggttc atgcaattta
     2701 aacagacaaa tcggtctgcc attgatcata ttttctgaca aaagctcgcc acactgttca
     2761 aacccgcgac gccagcgttc agcagtggcg ttttgatggc aacgcaaaga aatgtgatcg
     2821 gcagtcagcg gagtgatatt caaccccaga cggagggaaa gttcatctaa tgcgtgggta
     2881 aatcgcggta aatccgatgc aatatcctgc agctcgtcga tagattgcca gttcgccata
     2941 atcactcttc gtctttcagt aaaagcgtta atttaccctg ttgccctgcg ccaaccaacc
     3001 gctgatttca cgccgcttct gatgcaatag tgaaaacggc aatacgccac gcgcacgttg
     3061 ctgacgaaaa cagccatttg cagtatactc ccgccctaat ttctttaact ggtgcgggca
     3121 atttttgctc gcttcatcaa tgtaaggtat tccggtgaat attcaggctc ttctctcaga
     3181 aaaagtccgt caggccatga ttgcggcagg cgcgcctgcg gattgcgaac cgcaggttcg
     3241 tcattcagca aaagttcagt tcggcgacta tcaggctaac ggcatgatgg cagttgctaa
     3301 aaaactgggt atgacaccgc gacaattagc agagcaggtg ctgactcatc tggatcttaa
     3361 cggtatcgcc agcaaagttg agatcgccgg tccaggcttt atcaacattt tccttgatcc
     3421 ggcattcctg gctgaacatg ttcagcaggc gctggcgtcc gatcgtctcg gtgtttctac
     3481 gccagaaaaa cagaccattg tggttgacta ctctgcgcca aacgtggcga aagagatgca
     3541 tgtcggtcac ctgcgctcta ccattattgg tgacgcagca gtgcgtactc tggagttcct
     3601 cggtcacaaa gtgattcgcg caaaccacgt cggcgactgg ggcactcagt tcggtatgct
     3661 gattgcatgg ctggaaaagc agcagcagga aaacgccggt gatgaaatgg agctggctga
     3721 ccttgaaggt ttctaccgcg atgcgaaaaa gcattacgac gaagatgaag agtttgccga
     3781 gcgtgcacgt aactacgtgg taaaactgca aagcggtgac gaatatttcc gcgagatgtg
     3841 gcgcaaactg gtcgacatca ccatgacgca gaaccagatc acctacgatc gtctcaacgt
     3901 gacgctgacc cgtgatgacg tgatgggcga aagcctctac aacccaatgc tgccaggaat
     3961 tgtggcggat ctcaaagcca aaggtctggc agtagaaagc gaaggggcga ccgtcgtatt
     4021 ccttgatgag tttaaaaaca aggaaggcga accgatgggc gtgatcattc agaagaaaga
     4081 tggcggctat ctctacacca ccactgatat cgcctgtgcg aaatatcgtt atgaaacact
     4141 gcatgccgat cgcgtgctgt attacatcga ctcccgtcag catcaacacc tgatgcaggc
     4201 atgggcgatc gtccgtaaag caggctatgt accggaatcc gtaccgctgg aacaccacat
     4261 gttcggcatg atgctgggta aagacggcaa accgttcaaa acccgcgcgg gtggtacagt
     4321 gaaactggcc gatctgctgg atgaagccct ggaacgtgca cgccgtctgg tggcagaaaa
     4381 gaacccggat atgccagctg acgagctgga aaaactggct aacgcggttg gtattggtgc
     4441 ggtgaaatat gcggatctct ccaaaaaccg caccacggac tacatcttcg actgggacaa
     4501 catgctggcg tttgagggta ataccgcgcc atacatgcag tatgcttaca cgcgtgtatt
     4561 gtccgtgttc cgtaaggcag aaattaacga agagcaactg gctgcagctc cggtaatcat
     4621 ccgtgaagat cgtgaagcgc aactggcagc tcgcctgctg cagtttgaag aaaccctcac
     4681 cgtggttgcc cgtgaaggca cgccgcatgt gatgtgtgct tacctgtacg atctggctgg
     4741 tctgttctct ggcttctacg agcactgccc gatcctcagc gcagaaaacg aagaagtgcg
     4801 taacagccgt ctgaaactgg cacaactgac agcgaagacg ctaaagctgg gtctggatac
     4861 gctgggtatt gagacagtag agcgtatgta atcgattttt cgtgagagta aagcctgatc
     4921 agggttacct gatcaggctt ttttattgcc atctaaatgt attctgaaaa tggacatgcc
     4981 attgttttct gactgttgga taagaggcca gaagcgtaat atccggcccc agggaaacga
     5041 taacggttga atttaaggaa taccgcagtg tttaaatttc ttgtattaac attaggcatt
     5101 atctcttgcc aggcttacgc agaagatacg gttatagaaa acgaccatga catttcagcc
     5161 atcaaagatt gttggcaaaa aaattcagat gatgatactg acattaacgt gatcaaatca
     5221 tgcctgcgac aagaatacaa tctcgtcgat gcgcaattaa ataaagccta tggtgaagct
     5281 tatcgttata tagaacaagt gccacgcaca ggtgtaaaaa aacctgatac cgaacaactt
     5341 aacttgctta aaaaatcaca gcgagcctgg ctggatttta gggacaaaga atgtgaatta
     5401 atcctttcaa atgaggacgt tcaggattta atgtacatcg ttccacgtcg cttcgacaac
     5461 ggtattattc tctgccattt ttaccggttt gggttcggca ggcgcttttt gttcgcgtcc
     5521 gcgtatccac caggccagcc cgagcaatcc ggcagtgaac agcaaaaata ccaggttcgg
     5581 cattccaggc accaggccga gtaaaccgag cacggcggcg cttaacaaca taacgcttgg
     5641 gttactgaaa agctgattca ccatctgctc gccaacatcc tgatcggtgc tgacacgcgt
     5701 aacgatgacc cccgcggcgg taaaaatcac cagcgccgga atttgtgcca ccagaccatc
     5761 gccaatggtc aatagcgtat aactttccgc cgcgtgtccc atgctcatgc catgttgcag
     5821 cacgccgacc agcaacccgc cgacaacgtt aatgaccatg atgaggatcc cggcgatggc
     5881 atcgccgcga acaaacttac ttgccccgtc cattgagccg taaaaatcgg cttcctgagt
     5941 cacttcggag cggcgttttt tcgcctcatc ttcaccaatc aatccagcgt taaggtcggc
     6001 gtcaatcgcc atctgcttac ccggcatacc atcgagaaca aagcgcgcac ccacttctgc
     6061 gatacgcccg gcacctttgg taatgaccat aaagttgatg atcacgagaa tgacaaacac
     6121 cacgataccg atagcgaaat tgccaccaac gaggaagtga ccgaacgctt cgaccacctt
     6181 ccctgccgcc gccgcgccgg tatgcccttc catcaaaatg atacgggttg aagccacgtt
     6241 aagtgccaga cgcaacaacg tagtaaacaa cagaatggtc ggaaacgcag caaactcaag
     6301 cgtgcgctgg gtaaacatcg ccaccagcaa caccatgatc gacaaggcaa tattgaaggt
     6361 aaacaacagg tcgagtatga atgcgggcag tggcagcacc atcatcgaca agatcaacag
     6421 gatcaaaatc ggtccggcaa ggatctgcca ttgtgtcgat ttcaggtttg cgggcaggcg
     6481 cagcatcgcg gccagattac tcatgggtcg gtttctcgtt aataaaatcc agggcttccg
     6541 gcaccggaag atgagtaggt tgtacagggc gctgtccacc agccagacgc cagcgtttca
     6601 gttgccagac ccaggccagc acttccgcca ccgcggcgta cagttgaccc gggatttgtt
     6661 gaccaatctc cgcatgtcga tacagcgctc gcgccagcgg cggcgcttca agcgtcggga
     6721 cgttattttc agcgccaatt tcacgaatgc gcagcgcgac cagccctgca cctttagcga
     6781 ccactttcgg tgcgctcatt ttgttttcgt catactgcaa cgctaccgaa tagtgggtcg
     6841 ggttattgac aatgacatca gctttcggca catcggccat catccgacgc cgtgcagcag
     6901 ctcgctgcat ctgacggatc cgccctttaa catgagggtc accttcgctt tgtttgaact
     6961 catcacgaat atcctgccgc gacatacgca gctttttcag gtggctgaag atttggaaaa
     7021 agacgtcaaa tcccaccatt ggaatgacac caagcaccac cagcagtgcg catagcccaa
     7081 ccagatccat cgcattaccc atggcggtag tcggagactc ggccattaag cgcatcatct
     7141 gcggccagtg atgccagaga taaaaccccg tcacgctgcc aaccaggatg gttttcaaaa
     7201 ttgccttaag caactccgcg ccagtctgtg ccgagaacat ccgtttaatg cccggtagcg
     7261 ggttgagttt ggaaaacttc ggctgcaagg atttgccgct aaataccagc ccccccagca
     7321 tgaccggaga aatgagcgcc accagcacca cgccgctaat cagcggcagc agcgccagca
     7381 tggcttctct gatcagcaga ataatctgcc cgaggatcag attcgggtca
//
LOCUS       DAJTYZ010000175         7398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000175 DAJTYZ010000000
VERSION     DAJTYZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.175"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..147
                     /locus_tag="OO596_003713"
     CDS             <1..147
                     /locus_tag="OO596_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6719471.1"
                     /translation="NGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGK
                     RFER"
     gene            complement(500..1240)
                     /gene="phoN2"
                     /locus_tag="OO596_003714"
     CDS             complement(500..1240)
                     /gene="phoN2"
                     /locus_tag="OO596_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6719472.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(1569..2435)
                     /gene="ospB"
                     /locus_tag="OO596_003715"
     CDS             complement(1569..2435)
                     /gene="ospB"
                     /locus_tag="OO596_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6719473.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            complement(2685..2804)
                     /locus_tag="OO596_003716"
                     /pseudo
     CDS             complement(2685..2804)
                     /locus_tag="OO596_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            3048..3420
                     /locus_tag="OO596_003717"
                     /pseudo
     CDS             3048..3420
                     /locus_tag="OO596_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3982..4929)
                     /gene="icsP"
                     /locus_tag="OO596_003718"
     CDS             complement(3982..4929)
                     /gene="icsP"
                     /locus_tag="OO596_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6719474.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIDYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(6311..6463)
                     /locus_tag="OO596_003719"
                     /pseudo
     CDS             complement(6311..6463)
                     /locus_tag="OO596_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6519..7211
                     /gene="ospZ"
                     /locus_tag="OO596_003720"
     CDS             6519..7211
                     /gene="ospZ"
                     /locus_tag="OO596_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR6719475.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYGK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RLSQIKARMPVT"
     gene            complement(7235..>7398)
                     /locus_tag="OO596_003721"
     CDS             complement(7235..>7398)
                     /locus_tag="OO596_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6719476.1"
                     /translation="VGYVSFREAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWK
                     NSNSVASFC"
BASE COUNT         2248 a         1432 c         1409 g         2309 t
ORIGIN      
        1 aatggattgg ttgatgtaaa caaaaaattt caaaaagcaa atagtgggga cactatgctt
       61 gacaatgcaa tgaaaagcaa agattcaaaa atgattgatt ttttattaaa aaatggagcg
      121 gtatcaggca aacgatttga gaggtgacct aaaatatatt taggttgtgc cctgaatctg
      181 aagttgtcat ttctgttata ttgatcgaca taggggatgg tgcagaccgt tcccctgcaa
      241 caccacattg catgtggtga acggaaccgc cccgagtttc ctagagaatg ttttatctgt
      301 gaactcaggt tgccagatca tcgtttccga tggcagcata ataagctttt tctgcttctg
      361 ccggagggat atgtcccagc ctttccagta atcccttaaa ttcaggggta aaacgtggtg
      421 tgcccataga ctcctcctat gctcatatag ggtagatttg tctatgggcc ctccatagcc
      481 tgatacaggc tgtccagctt tatggggtca gttcattggt aggagtattt aattcttcaa
      541 actctttttt ggcttcgcta aggcttttgg taaattcagg tgtattatga agtactgcaa
      601 caaccgatgc tcccattaaa cgcccagcct ctacatcgct ttgccaatgc gcaccgcaga
      661 tgacccgact ttctccaaac tcatatccac gtcgaagtat ttccgcttta cgttgaggat
      721 taatctccgc aagtatcagt gctactgccc aaccaaagga tgcatgacca gagggataag
      781 agccagtgat agccatgttc tcatctttat caggtgtaca ggttgcgtct ttataaataa
      841 cgaatggacg aactctttta taatattctt tggcgttacg cacggcgtaa tcatggctat
      901 cctgtagtac ttgtgttaac aactgatata agattggggt attttccctt gaaatatcca
      961 tgccataagc atctgaaaat gcaagaccaa aattctcgta gtatgcatct tcgctagcga
     1021 gacgtacacg attagcatcc cggagcgagc ggccgaattc ataatgagct ttgtcagcct
     1081 gaaatactac tgaatcctct gccggaggcg gcggaagtat tgacaaactg tctggtgaag
     1141 tttgttgagt gagaaaacct tctgccttca gagcatttgc tgaggggata aaaatcatat
     1201 ttgtagcaat acaaaaaaga agaaagtttt tggttttcat aatttatttc cttagcataa
     1261 ttaattgatg attttggtca gctgtaaaaa acgagctgtg ttacaaaaaa cacaagtcat
     1321 gcggaaaaat ttgaacagag tgatggaaaa acaaaaaata gtcttatatt aaagtaataa
     1381 cattattttt catattcacg aaaaataatg tggtaaaaga atgatgtttt ttatctattt
     1441 gttcgtatcg ttttgtctct gtattatgat gatttgtgat tttccctcat agggaagcac
     1501 ttttctacta aagataaaat aacttcatct taaatgcaaa caattaacta taggttaaaa
     1561 attttatact aatccagttc tttattaata ataaaagtct ttttctgaga gcttcttttt
     1621 actgtatgct ccggatcagc aggaattgaa gttgaacgat aatgtgagta gggaaaaagc
     1681 tcttgaccat agtgaactcc atatccatga tagccggata tttttagacc atcactcaat
     1741 gactcatcgt tttcaaggtg tttttttatc tgctctagca aagattcttt ttccttaaaa
     1801 aaaggcagag agttaaggat acaagaaaga ctttcagcag gggcattgtt aaaattttta
     1861 ggagccactt tatcagcgga gccgcatgaa gtaaaacgga tatctttcac atttagaata
     1921 ccacagtctt tcattcttga aatgattgtt tgtacatcaa taagttccga accaaggcct
     1981 aattgatgag aaccaggact accgtgccca agaatataaa ccttattata ctctttaaaa
     2041 tcagtatcgt ttactgtttt tattttcgct ttttttacag aaaaataagc agcagcattc
     2101 aatgcgtcat catcattatt tattactatt ggttcactct gtggtagcca tgattcagcc
     2161 atttttagaa aatcattttt acttttttca tttttaaaaa cataatcata taacatcttg
     2221 ttcttaaata ctcttggtat ttgttgtccc ataatagata gaacatcatt gctatcacaa
     2281 aaacctttct ctcctaaaaa aaccaatgct gcttttgggt gagtacatga tgaatttttt
     2341 acccttttta ttatatgtgc aaatgcaata tctgatatgc tttcattctt tttattgact
     2401 attctacagt atggtctaac accatctaaa ttcataccta catgactcca tatattgttt
     2461 tataaacatc atagtaaagg gtgttatatt ttcatatagt atgcaagctt ctttgtatac
     2521 tatgatttaa aaaaacggaa caaaaatgca attatattct ggcattgttt aatgctgttt
     2581 gtctttttta ttcattattt caatgaaaga atttcaacta caaagttaat tgatcctacc
     2641 tacataatat agacacagta cctaaagtac cgtggagatc atccggggaa gccccgggtc
     2701 tgataccagt gcaggtagac cgctatcttg ttccggtaat gttctgccgt tctttacggc
     2761 atcacctcta gcagaaacat cgcgtaactg cgccaagtat gatgaacttc tcttaccgct
     2821 gcgatctggt tcaacaatat gcagatggta cacaacctct ttaaccagcc catccggatt
     2881 attcagagcc gcttccgcaa tccggaataa aagtgctgtt ttaccgtgaa ccctctgaaa
     2941 atcagcgagc agtgttttaa ccagccgacg ttcagagcgt actgacagac ggtggacaat
     3001 ttgaataaac agttcaatca gaccgtagat gctctcccgt tggcgacatg ctgggagcgg
     3061 tggaacaccg cttcggcaac gagcttccgg cgtctccagt gaagtgactg acggataatg
     3121 gttcatgcta ccgggctaat gaaacacgcc agttcacccg gatgttggga ctttgttgaa
     3181 ccgaagaaca cggcggtgcg gagtccggag agtaacagaa tagcaaagag cttcgtgaaa
     3241 acaataaagt gtgactacat cagtatcatg cccaaacctg acgggttaac ggcagcaaag
     3301 aaccttgcag aggcattcga gcattataac gaatagcatc cgcatagtgc gctggattat
     3361 cgctcgccac gggaatatct gcggcagcgg gccaatgata acaggtgtct ggaaatatag
     3421 gggcaaagcc acccacgaac gatccagcat ataatcaaga aattagattt catttaacat
     3481 aagatagatt atgtgcacca agaggcgttt ccttaatttc tgaatttaca ggctcttttg
     3541 ctctctttgc tatgctgtgg atttggaaag atagagtgct ggctgattat ttaggtattt
     3601 tcatcagcat gtccctgata gcactgttcc atcagcgctt tcaggcgata atagtagacc
     3661 ttgggtagcc tctgcccatg tgtaacttga taccccgaga acagccacga tgatctaagc
     3721 ggttcttatt tctccggtct cagggtcggt aatgccaaca gtaagtccac agtaatcaac
     3781 gaagagtttt ttgaaccgcc ccggaaatcc tggagactaa acgccctgag aaagaggtaa
     3841 acagaatgac taaaaataca cgtttttccc ccgaagtccg tcaacgggca gttcgtatgg
     3901 ttctggaaag tcagggcgaa tatgactcac aatgggcggc aatttttagc catgaaaacg
     3961 taatcaactc ggtgaaccaa ttcaaaaaat atactttata cctgcggaag tgagaaaacc
     4021 aatatattca atacttgcac tatttttaat ggtgccagat atattggtac tttgctcata
     4081 aagagatgta tcaccttttt tattagaaat gtaatttctg gagccttcta tataaaattt
     4141 tgcactgggg gtgacatagt atccaatatt cgcagcaaca gagcagtaat tttggttttt
     4201 tatttcatct ttaaaaattg tctcagtctg atagtgttta tctgtatcag atgaacgaac
     4261 ccaaccacta tatttcagtt ctgctccaaa ctcaaaattc tcatggcggt aattagcagt
     4321 taatccaata taaggtattt tgaagagctg tttgtaatct attgttcttt caccactagg
     4381 atgtgcccct tttttattcc tgttcccccc gttttcacta taaatataac tccctcccat
     4441 tgcattaaaa ctgtaacgac tctcttggta acctgctatt agtccaagtc gataatccaa
     4501 attatttaat aaccaacctt taagattcag ctcgaattcg ttagcatcac ggagatgcgt
     4561 attgggatgc cagctctggt cggtccatac ctgaggggta ttggaattgt tccagtcttt
     4621 atcaaccatg cttgctttct ggttacctaa agtagtccaa ccggaaactc cgaaagacac
     4681 ttttggaatt agcttccaat cgatgccacc tctaacaata gtcgcattac tgtatttcca
     4741 gtccagttga ctaatttttc tccccccttc cttgggatga taaacgcgtt cttttgtttt
     4801 gccactcaga gatcctagac taatatccgt actgatgttg tccggtatga aaagtggata
     4861 gttagtggta gcatgtgtag taaagatcgc tggtacacaa agtgcaagta caaggaattt
     4921 taatttcatc gagaactcta cttttttggt tgaaatgtcc atgatcttct tactttataa
     4981 gataaacgtc aatgccaagc attattttaa ttaaatattt gtttatgtta ccaagaacat
     5041 atcttttatt ataatattta ttgaactttt gtaaaaataa aaaaaatacc tcattgtgcg
     5101 aataaagtaa cgggggccat agatttctct acctaccttt tggtgttcat acctgaggct
     5161 gatagtttca ggattccgcg acaattctac tcaccttctt gttctctcgc caatatgaaa
     5221 tcaatcagaa aagcgttcca taagaacaac gataagacaa taaattgtca tcacggctgc
     5281 aatagccaac gtgcctttca gtaccaacat gacaacctcc tgtatttgac gtaacgtgtg
     5341 ctgataaata tgtatttgta tctcaactga ctcaatacaa cacttaagta tagtttttca
     5401 gattttgttt ttcccgtgaa atattatgga aatggcagaa gcaatacatt gaattccgat
     5461 tcgagtgaat acggctagcg ggcacctcac tttagcactg aagccatcaa cttttatttg
     5521 acttatttca atggaaagct ttctcactat cagtacaggt agttcccatc cttttaaagt
     5581 cgagagtcag actgcgacta atacagttaa agtgaccaaa ctcagttatc ctctacacat
     5641 tttacctcat gtttacttgg acaattcatt ggtgctttta taaaagaata aatattctac
     5701 taatttaatg catcacctaa aatagtattt tttataatag aactgtatct atagtatatt
     5761 attaaagctt aattatttat tatgctttaa tgtggttgtc cgattaagga ctaatccttg
     5821 aaaacgccgt atagggcggg agttcactct attgcgataa cgtaaccgta caatattgtg
     5881 cgtttcccaa attgaccact actgaactat tctgggattt aaatgcaata aaatggttgg
     5941 ttgaaggtcg tggtgatgat gttagtcctt ttgagaatat tccagtgcca gatcttactt
     6001 ataataaaga ggcttggcag tttggataag ctattgtcct tattccacta atacaaactt
     6061 tttgtaaagt atattaaata tacttcattt catactgaaa tccccggaag tattcaatca
     6121 aacaattaca ccataaaagg aagagggagc cccaattaag ttatcaagca tgtttatgat
     6181 acctgcaggg tataaatttt ctggctgcaa gcagacacat ataaaacgtc tttattgatc
     6241 ctacccacat aatatggacg cagtcgtaag tgaggctctg tgagcctacc cacgtaatat
     6301 ggacacaggc ctaagcgagg ttctggtttt caaattgttc cggactgagg ccgccacacc
     6361 aacggtgccg tcgccagcga ttgtaatcgc actcgatata attaaacact gttgcccgca
     6421 ctatttcccg atagataaag tgttctccgt ggagacattc cacttaggaa caattatatt
     6481 gttcatggta atgtttatat ttgagtatag agattaaaat gattagtccc atcaagaata
     6541 ttaagaacgt atttccaata aatactgcaa atactgaata tatagtgaga aatatttatc
     6601 ctagagttga acatggttac tttaatgaat caccaaacat atatggcaag aagtatatct
     6661 ctggtataac cagaagtatg gcacaattaa aaatagagga gtttattaat gagaaatcca
     6721 gacgacttaa ttatatgaag acaatgtatt ctccatgtcc ggaagatttt cagcctattt
     6781 ccagagacga agccagtaca ccggaaggaa gctggttgac agttatatct ggaaaacgtc
     6841 ctatggggca attttctgtg gatagtttgt accatcctga cttacatgca ttatgtgagc
     6901 tcccagagat tagttgtaag atcttcccta aagaaaatag tgattttttg tatataattg
     6961 ttgtattcag aaatgattct ccccaaggag aactacgggc aaatcggttt atagaactat
     7021 atgatataaa aagagagatt atgcaagtgt taagagatga gtcgccagag ttaaagtcta
     7081 ttaaatctga gattattatt gctcgtgaaa tgggagaact ttttagttat gcatcagagg
     7141 aaatagatag ctacataaaa caaatgaatg acaggctttc tcaaatcaag gctcgaatgc
     7201 ctgttactta atgtatatta attgtatgaa gtggtcaaca aaaactggcc accgagttag
     7261 agtttttcca gtatcgattt tccgattcgt ttgggggtaa cccaccgtta tattcgtgcg
     7321 gtcttagtgc gctgtaatat ccaacgatat agtccgttat ggcgtgagct gcctctctga
     7381 agcttacgta acccacca
//
LOCUS       DAJTYZ010000176         7163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000176 DAJTYZ010000000
VERSION     DAJTYZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.176"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..4274)
                     /gene="traI"
                     /locus_tag="OO596_003722"
     CDS             complement(<1..4274)
                     /gene="traI"
                     /locus_tag="OO596_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR6719477.1"
                     /translation="MMSIAQVRSAGSADNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVTNVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKRKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     MYTDLLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLT
                     LRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     VGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLERVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKL"
     gene            4356..4583
                     /gene="vapB"
                     /locus_tag="OO596_003723"
     CDS             4356..4583
                     /gene="vapB"
                     /locus_tag="OO596_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6719478.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            4583..4981
                     /gene="vapC"
                     /locus_tag="OO596_003724"
     CDS             4583..4981
                     /gene="vapC"
                     /locus_tag="OO596_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6719479.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(4990..>7163)
                     /gene="traD"
                     /locus_tag="OO596_003725"
     CDS             complement(4990..>7163)
                     /gene="traD"
                     /locus_tag="OO596_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HCR6719480.1"
                     /translation="LPRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCI
                     YWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATV
                     VALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVARMLKKDGRDSDIRIG
                     DLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPS
                     IDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAY
                     LMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVK
                     AIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIR
                     GLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYG
                     EKAAATLFDVMNTRAFFRSPSHKIAEFAVGEIGEKEHLKASEQYSYGADPVRDGVSTG
                     KDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQERPKVAPEFIPRDINPEM
                     ENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQPQQPQQPQQ
                     PQQPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMA
                     AYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT         1485 a         2207 c         1838 g         1633 t
ORIGIN      
        1 agtttccggt taccttccgt gccttcaagc gcgatggcaa aggtttcact cgccccctgt
       61 gcaccgtggg cggtgatggc ataggccagg tcaatatgtt gctctgcccg ctcctgaccg
      121 gggcgaatca cacgggtctg ctgaccgtca gaaagtgtga cactgtcacc ggaaaccgct
      181 gtcaccgtcc agacgctgtt ggccacataa ccgcgctccc ggtcactctt cgtgaagcgt
      241 atcctgtcac cggtccccac ccggatggtg tccggggtgt acagcgtgac gccttcagcc
      301 accgcctccc ggggcgaaat cagccgcgtg ttaccttccg catcctgcag ggttatcagc
      361 ccgtcatcct tgctgatacc ggcaatccgg tgatacacat tatccaccag ggcaagggca
      421 tccgggttat tctcccaggt ggagagacga cgcagctccc cgtcgcgtat attcgctgtg
      481 ttcaggacag gcaccatgac ctgcacctgc cccagttctc cggccttttc ccgtgcatca
      541 tgaatcatgc tgttcagtac gcgccgatcc tcattcaggt gcgtgacaat cagcgtctgc
      601 tcccgtgctt ccggtgtcct gccggtatag tcgcggacaa tggcttcata cagtgtcatg
      661 gggacatccg gaaaagcctc gcctttcagc atcgccttct gctgcgcttc tgccagtttc
      721 gcttcctggc tgtgactgaa ctccgtcacg gagtgctccg gtgcccatgc gccctccaga
      781 cgtggcacct gagacggttt cacacgctca agcccggaca gcgccctttc cacatcccgg
      841 ttaatcaggc tgtataccgc ctcccgcagt tccggcgtct gacgcacaat ctccttcatg
      901 atgaccacat cggcagcact gcgcgtctgc tggagacgga aaggctgacc aggcgcgatg
      961 gcctgcagct ggtccgtgtc accgctggcc acagcccgac cgccaccgac cgcaatcagg
     1021 gcgtatgccc gtgccatgtc ggtattgccc accattgagc tctcatcgag caggaacagc
     1081 gtgttgctga aatccggcgt ttctccgctg cgctgctgca gctgcgtgtc atgcagaaag
     1141 gacgccagtg tctgcgcatc cacgccggcg ctgcgcatct ccccgaccgc gcggtgcgtg
     1201 ggacccagcc caacgacacg gggacgttca ctctccggca gcatgttcac ggctgacatc
     1261 accgcccgga actgtgtggt cttacccaca ccggcatagc cctgcaccac cgtgaaacgg
     1321 tcggacgttt ccagtatcat acgggtggcg gcgcgctgtc ctgatgtcag tttctccatg
     1381 agttcgccag gtactctctc catcagcggc gtgaccgcct ccttaccttc gagaatatgg
     1441 cgaagaatgc tcttttctgc ctcatacgac gcacgggaaa ccagcaggcc tgtgccatag
     1501 ccttttgcca catccacata cagtaaatca ccccgtttta tctgcgcatt gatttcccct
     1561 cccaggtcag caaaaccggt tccttctgca gcaaacgact tcgcctctgt cagcaggtcc
     1621 accatgctga aggccaggtt tttactttcc agcacaggaa gggccagatg aatggcctgc
     1681 tgtgccggcg tgtgaagccc ggctttctgc agactgatag cggtttccag caatgtctca
     1741 ccggcacgcg cctttatctg ctcagaaacc accgtaaagg agggatggcg ggcaagtttt
     1801 tccgcagtac gggtttcatc cagtgaggaa tacagccgga catcacgacc actgcgggcc
     1861 agaccgttca gggtggcatt gtccattgcc atctgtgtga cggaggcaaa aaccgtcgca
     1921 ctgtcgctga cggaatgccc gggcgtttcc acccagccgt tctccagctt cagtgccgtg
     1981 aacggtgaat cgctcacagg cagggtggcc ggttcagccc gtcccggcac aacaaccgtc
     2041 atcgcatctt cactgacgga tgccacctgc aggcggtcac cgccggagac gcggagtccg
     2101 ggaattttcc ctgtcaccct cagacgctca ccgtctgcca ccggcatttt ttccggccgg
     2161 aacagcgacc agctgctgtc cagggaagaa atacgcacca cctgcgtttc gccctgcgca
     2221 ttccgcaggg tcaggctgtg actctgcgcc gtcacacgtt ctgtcacata gcggtcatga
     2281 ctgcgtgtct ccgggttcca ctgctccatc accattcccg ggcggtacat atcccgcaga
     2341 taacggctcc ggctgtccag ccagaccggt gacagggcgg tcatggttac ctccgggtgt
     2401 ccgagcacgc cctgtgtttt cagctcactg cgaatggcct gtgtcagtat ggcctgttcc
     2461 cgtactccgc tgacctgtgc cacgctctct tctccggctt tcacgctggc cgcaaaatct
     2521 ccggccagcc gggcatagcg gacattacgg tccggttcac tgatgatggt ggccggtcgc
     2581 tgttctcccc cctgccagcg atatgtgttc accccggcat ccttcatggc catcagtgca
     2641 ctgcctgtac cggttcgctg cccgctgtcg gttatcagga cctgtacgtt atgacgtgcg
     2701 gcaccatcca gcagggttaa cgtctctttc agggagagtt tttcgccctg gtcgacgata
     2761 acggtactgc ccggcgtgaa ggccatgcct tccagcaact gacgacgtcc ggttatcagt
     2821 tcaccggaca gccgttcatc ctgcttcagg ttcatctgcg agcgacggtc agcagcgata
     2881 atctgcacct cccgcccctg ctcccgggcc atcatgacca gttcagccac ccgctcacgc
     2941 tgcccggctg caccgccctg cccggacaca atggccagcg acggacggtc ctgtgccaga
     3001 acactgacgg catcgctgta accggccgtc cgggggacac ttttctccgg atgtacggtc
     3061 acccggttct gtttcatgat gtcacgactg agtgcccgga ctgacagctc atcgagcata
     3121 tgaatcccgg acgtgaacag ccccttctca cgatcgaggg ggataagctg ctcacggctg
     3181 atggcctcat cgataccggc gcgtgcccgt tcaatcacac cattttccgg cggcagtatg
     3241 ccaaccgtcc tggccagcag gtccgtatac ataaactgca ctttgcgttc acttaatccg
     3301 gcaatggcct gtgtcaccgc ctgctgcaca tccggcccgt cctgtgaagc gggtccgggt
     3361 gtctgcgtgc gggtatatgc ccgttgttcc gctgcgtcac gatatgcccg gatgtcgaac
     3421 ccggtttcct tcagcgtctg catccactcg gccattctga cttcaggatc gacgtgctgt
     3481 ttggatttac gcgtatccag cgccgccaca tcccgggatt tcagcgaggc gtcttccccg
     3541 acggcctccc ggatagtctg tgagcgtccg gaaaaggcct ccaccggtac acccggcatt
     3601 tcccacatac cgtgcttacc gaccacttca gtttcatagc ccagcgcctc aacctgctct
     3661 ttccgttttt cccggtagag cctgccaaag gcaatctgat tagcgtacac attctcaatg
     3721 aaccccgttt tccccacttt gtcactgctc agtgtcttcc attcgccgtt atgctgcgta
     3781 acattagtca ccaccgcatg cgtgtgtaac tgtggttcct gatcgcgact ggtgtcgtgg
     3841 ttaaacagtg ccatcaccag attaccggtc agcaccgttt ctgactgtcc gtccgtcatc
     3901 acccgtgtgg aggccagtgc ctccacctga cggacagcaa aatccacggc ctggttatgt
     3961 gcttcaataa gacgtttgtc ccccccgagc atggccatca tggagacact tttgggagcg
     4021 gagaaggtca ggtcgtagcc gggacgatgc ctgttactgc catcctgcat gcggcttaaa
     4081 tccgccccgt ccggcagcct gccctccaga aggcgggtaa agacatcctt atcgacactg
     4141 ccctgcagcc ccagttgttc agccccctgg ccggcccagc gttctcccat gctgcccagc
     4201 acatagtaat tatccttgtc ggtataataa ttatcggcac ttccggccga tctgacctgc
     4261 gcgatactca tcataaacgt atatcccttt gacatatccc ggcatcaatc ccacaataga
     4321 tatacacaag acatatccac ataaggaggc aaataatgga aaccaccgta tttctcagca
     4381 accgcagcca ggcggtcaga ctgccaaaag cggttgcatt gccggaaaac gtaaaacgcg
     4441 ttgaagtgat tgctgtcgga cgaaccagaa tcattacgcc agccggagag acatgggacg
     4501 aatggttcga cggacacagc gtcagcgccg attttatgga taacagggaa cagcccggca
     4561 tgcaggaaag ggagtcattc tgatgctgaa gtttatgctc gataccaaca tctgcatttt
     4621 tacgataaag aacaaacccg ccagcgtcag ggagcgtttt aacctgaacc aggggagaat
     4681 gtgtatcagt tcggtcaccc tgatggagct gatatatggt gcagaaaaaa gccagatgcc
     4741 tgaacgtaat ctcgctgtga tcgagggatt tgtttcccgc attgatgttc tggattacga
     4801 cgctgctgca gccacacaca ccggccagat aagagcagaa cttgcccgtc agggacgccc
     4861 tgtcgggcca tttgatcaaa tgatcgcagg tcatgcccgc agtcgggggc tgattattgt
     4921 gactaataac acccgggaat ttgaacgtgt gggcggcctg agaactgaag actggagctg
     4981 acctgttcat cagaaatcat ctcccggctc aacatcctcc ccgcgttccc ggtgcacatt
     5041 gatgttcacc tcttcacgac gctgcatctg ctgccaggtg tccggatgat tttcctgctg
     5101 ccatgcctca taagcggcca tatccaccac ttcaccggat tcactgatcc cgggtggcag
     5161 ttgctgttcc atctcttctt ccggtttcat tttcagctcc tgctcaatcc cccctgccgg
     5221 aacattcaca cctgaatctg acttcttatc gttgatgaca ggagacaccg gctgttgcgg
     5281 ctgttgcggc tgttgcggct gttgcggctg ttgcggctgt tgcggctgtt gcggctgttg
     5341 cggctgttca gcctgagtca cgtcttctcc ggaaacaacc tccgggacat ccggttcgaa
     5401 gaggctggcc atctgacgac cttctgcttc ccttgcggca agtacggcac tcagacggtt
     5461 ctccatttcc gggttgatgt cacgcggaat aaactccgga gcgaccttcg gacgttcctg
     5521 atatttcagt gagagtttga ctgccggata cggtccgggc agggtgacat agcaggtcag
     5581 atccggcagg gactgaatgt cggaataact gaccagcgtc tggcgctcca tatccttacc
     5641 ggtcgatacc ccgtcacgta ccggatcagc accgtaggaa tactgttcgc tggctttcag
     5701 gtgctctttc tcaccaattt caccgacagc gaactccgca atcttatggc tgggagaacg
     5761 gaaaaaggca cgggtgttca tgacgtcaaa cagcgtggca gccgctttct caccgtagat
     5821 atcttccagc tgggcatagg actggatacc aaacacataa cagccaccga acttacgggc
     5881 ttccggcagg atctccacca ggtccggcag tttgtgtaat gtgggtaact catcacagaa
     5941 gaaccacaca cgacgattac ggttttctcc cattgccagc agaccacgaa tggcaatgga
     6001 cagccacatg gagatcaccg gcttcaggga ggcatgagtg tcggcattcg acgagataaa
     6061 cagccagccg tttttctgat cctcccggac accacgcatc cagtcacgga tggtgaaggg
     6121 ctcaccgtta tgctcaatcc cctgcaggta acggatggct ttcacatagt tggtcagcac
     6181 agcacggatg gaaatcgccg ttttctcaat tttctcttcc accaggttgg ccgccggtga
     6241 attacgcagg taggtacgca gtttttcaat tttgatggaa agcagtgtgt ccaccagttt
     6301 gctgtagctg cggttggggt cattacgcat cagatacgcc gcttccgcaa aaatggtacg
     6361 tcctgaaccc tgccagaacg ggtcctcttt tgtgcccatc gggatcagag tatttgcggt
     6421 attatcaaaa tccggctgtg tcaggcactc cttccacaaa tcccaggcgg cacagcgtgc
     6481 atccagcgga ttcaggatct tatcgatgga gggatcatag taacttttaa caaattcccc
     6541 tgaacggtca taaatcacca ccatatcacc acgctgacgg gcgtagttgg ccagacgacg
     6601 gataacctcc gacttaccgg ccccgaccgt gccgtgcaga cagaagttct ggatttcgga
     6661 atcccggata atcggcagat cgccaatccg gatatcggag tccctgccgt cttttttcag
     6721 catccgggca acgtctttcg gattgtctgt cagttgacga ccacctgtga cttcattttc
     6781 gctctgttgt ttaccctgac gccccagaat ccaggagaca acaaagaagg taatcaggca
     6841 aataaccagt gccacaactg tggccagaac gaatgcagac cataactgct caccgcacca
     6901 gatcatatat ttatcatgca gtacctgagc agcgttcatc cggaaggttt tgccgtaata
     6961 ctgaatctca tataccggct gagacttgat taaatcccgc atgccttcca gcgtggtaca
     7021 ccaccagtaa atacagccat tcacaaacgt ctgccagctt atttttaccc ataaaaccag
     7081 accaacgagt atccagaaaa aaataaacag gcagtaaagc ataatattgg cgatctggct
     7141 gaacatgcgg atacgcggta atg
//
LOCUS       DAJTYZ010000177         7049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000177 DAJTYZ010000000
VERSION     DAJTYZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.177"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..302
                     /locus_tag="OO596_003726"
     CDS             <1..302
                     /locus_tag="OO596_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6719481.1"
                     /translation="QRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVP
                     SSAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(391..903)
                     /locus_tag="OO596_003727"
     CDS             complement(391..903)
                     /locus_tag="OO596_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6719482.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1098..1862
                     /locus_tag="OO596_003728"
     CDS             1098..1862
                     /locus_tag="OO596_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719483.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1813..3216
                     /locus_tag="OO596_003729"
     CDS             1813..3216
                     /locus_tag="OO596_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6719484.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3216..4682
                     /locus_tag="OO596_003730"
                     /pseudo
     CDS             3216..4682
                     /locus_tag="OO596_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4573..5316
                     /locus_tag="OO596_003731"
     CDS             4573..5316
                     /locus_tag="OO596_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6719485.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5320..6540
                     /locus_tag="OO596_003732"
     CDS             5320..6540
                     /locus_tag="OO596_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6719486.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6544..>7049
                     /locus_tag="OO596_003733"
     CDS             6544..>7049
                     /locus_tag="OO596_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719487.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1866 a         1683 c         1930 g         1570 t
ORIGIN      
        1 gccagcgtga tgctgatgca ctcgatgcta aatacacgaa ggagttagct gatgcgaaag
       61 ctgaaaatga tgctcttcgg cgcaagcttg ataatggtgg tcgggtgctc gtcaaaggaa
      121 aatgccctgt gccatcctca gccgaaacct ccagcgcctc cggcatgggc aatgatgcca
      181 ccgtcgaact ctctccagtt gctggacgaa acgttctcgg tatccgggac ggaatcatca
      241 gcgaccagac agcattgaga atgcttcagg agtacatcag gatccaatgc cttggggggt
      301 agcggtaatt ttactcatta tccttcaaat cagattctgt tgtcagagga atgggggaag
      361 ctggattcgc aagagctttt tatgggcagt ttagctaaaa aaaggcaaca cgaagctaaa
      421 actaccaaca taaagtgctg accagctagc agctgaaatc agactcactg tatacacttt
      481 cactggatga agttgcaaca ttcctgccac caaaggagca atataacgca gcacggcgat
      541 aaagcgcgaa gtgaaaagta ttgacacaga gttattttgc aactgaaggc gaacccgttc
      601 tattgtattg gaacggcttg atattataca tgcaagttga gggatatgcc caattattat
      661 accaagatgg taattaacga tagtaccgca ccatgcgcca ctcatgaccg tgattccagc
      721 ttcccatggt gaaagagtcg tcttgctgac tgtaattaca gtggccatca taattgatgc
      781 tggtggcaat atcgccgaaa tgagtaaagt ggattttgtg aacgctatta taaaaagaag
      841 cccccatagg cgatatggat gaagtacaaa atagttcatc aaagcattta tccactccat
      901 caatgtcttt ccttctgtga atttttcttt gctatttaac cgtgatacga cttaatttag
      961 gcttaaacac cttacattta caaggtactc ctgtgggggg ccctgccacg gagcgtcgca
     1021 ggagtgagtg atgaggaaga attaaagcaa cctattgtga caggcagcga taacagtaaa
     1081 tcacaggaag cataatcatg gcaaaaccgg actgggaggc catcgaatcg gcataccggg
     1141 ccggagtcct tagcctccgt gatataggcg agaaatacgg cgttacagaa ggggctatca
     1201 ggaagagggc caaaaagctt ggttgggcac gcagtggcgg tacgcaggtt tgcaaaaatg
     1261 gtacgcaaaa aaggaaagtg cgtaccagca gaaagcctgc cattactggc cttacacaaa
     1321 aaagtacgca actaaaaaca gaatctacac cggatacgaa accgatacgc ggaatgcgta
     1381 ccgatccccc gactaaccca ttccaacccg gtaaccagca ggcattaaaa cacggtggtt
     1441 atgcccgtcg ccttctgctc aaagatgagg tgatagagga cgctaaagcg ttgacgctcg
     1501 aggacgaatt atttcgcctt cgtgctaaca accttgtcgc tgcagagaat attggtcggt
     1561 ggttggtgtc gctggaagat gctaatgggg accaggaaag gaagatgctg attgaaaata
     1621 tcagcgccgc cgagaaagca atgatgcgca atacagttcg tattgagtcc atcgttggca
     1681 cgcttgcgac ggtaggaaaa atatttgctg atacagccta ccgcaaggcc gccactgata
     1741 aggtgtctct ggaggctgat cgtcttcgcc gtgatgcagg tattgatgat ggcaatggag
     1801 agcgtgacct caatgacttc tactctgaca tccaaaccga cgctgaatcc ggctttacgt
     1861 agtttctgga ctatgcgggc acgtaacaaa gtgctttatg gtggtcggtc atcgtcaaaa
     1921 tcatgggatg ccgctggcat tgccatattt ctgtcgaata aatacaccct gcgtttttgt
     1981 tgtgcccgtc agatccagaa taaaatcgaa gagtcggtgt ataccctgct caaaattcag
     2041 atagacaggt ttggtctgcg gcaccgtttc cgtattctga acaacaaaat cattaaccgg
     2101 gttactggct cggaatttgt tttttatgga ttatggcgca acatcgaaga aattaagtca
     2161 ctggagggga tcgatgtgtt gtggctggaa gaagcccacg cactgacgga ataccagtgg
     2221 aaaattctgg agccaacgat ccgtaaagag ggttcggaat gctggttcat attcaacccc
     2281 ggacttgtta ctgatttcgt ctggcgcaac ttcgttgttg atccgcccga aggcactctc
     2341 atccgcaaaa ttaactatga cgaaaatccg tttctgtctg acaccatgct taaggttatc
     2401 gacgcggcgc gacgccgtga tccggatggt tttaaacatg tgtatgaggg cgttccggag
     2461 tctgatgatg atgcggcaat catcaaactg tcttggatag aagccgcagt ggatgcgcac
     2521 aaaacgttaa atttcgaacc cagtggaaga aagcgtattg gctttgacgt ggctgacagt
     2581 ggtacagata agtgcgctaa cgtttaccgt cacggatccg ttgttttctg ggccgacgaa
     2641 tggaaggcca aagaagatga attactgaag agctgccagc gtacttatca ggcggcgctg
     2701 gagcgtgaag cagatattgt ttacgactct atcggtgttg gtgcgtctgc cggtgctaaa
     2761 ttctctgaaa ttaacgctga ccggaagagc gagaacgcat acgcgcgacg tgtgaattac
     2821 cagaggttta acgccggtgc tggtgtgcat gagccagatg acgagtacaa cggcatcccc
     2881 aacaaagact ttttcgcaaa tcttaaggct caggcatggt ggctggtggc tgaccgtttc
     2941 agaaatacgt ttaacgccat taacaacgga gaacagtatc ctgtggatga gctgatcagc
     3001 atagattctc gttgtccgtt gcttgaaaag ctgaaactgg aactgacaac acctcatcgt
     3061 gatttcgacc gtaacggacg tgtgatggtc gaaagtaaaa aagacctcgc aaaacgcgag
     3121 ataccgtcac caaacgttgc tgatgcattc attatggcct tcgcgccaat cgatacatcg
     3181 ctggatatct gggaacagct ggggagacag gcctgatggc acgaaacaaa caagccctgc
     3241 ggcgaactgt gcaggccaca gctgatggtt atgagaattt tattgcccgc gtagggatgc
     3301 agacacctaa ccagcactca gcatccacct accgggctaa tttcaccagt cgtaaccgca
     3361 tgctggtgga atggtcctat cgttcatcct agatcatcgg cgaagcagtc gatgctatcc
     3421 cggatgatat gacccgcaaa ggcattcgca tcacttcgga aattgatgca aaagatcgtg
     3481 gcattctcga atcacaactg gatgagttgc aaatctggga tgcgctgaat gacgtgctga
     3541 aatggtcgcg cctctacggc ggcgcggtgg gtttcatcat gattgagggg taggcaccaa
     3601 tgaccccgct gcgacccgaa accatcggta agggcaagtt taaggggatt ctcccgctcg
     3661 accgctggat ggtcgacccg gcactgaccc gccgcattaa agatatgggg ccggacctgg
     3721 gtaaacctga gttttacgat gtggtgacca cagcaacggg aattcctgcc tggcgcattc
     3781 atcacagtcg cctgattcgc tttgatggcg tcacgttgcc atttcagcag aagatgaccg
     3841 agaacgaatg gggaatgtcg gttgtagagc gtatctggga tcgtcttacc gcgttcgaca
     3901 gcgctactgt cggcgcggcg cagctggtct acaaggcgca tttgcgtacc tacagcgtgg
     3961 agaagctacg cgagcttatc gcacttggtg gtcctgcgta tgaagcgttg ctgaagaata
     4021 tcgacctgat tcgacagttc cagagtaatg aaggcatgac gctcatggac tcgcgggata
     4081 agtttgaaac ccatcagtac agcttcagtg gtctggatga catcctttcg cagtttgtag
     4141 aacagattag tggcgctgtt ggtatcccac tggtgcggtt gttcggacag tccccgaaag
     4201 gattttctac cggtgatgca gaccttgcca actattacga tcgcatcagt tcgttgcagg
     4261 agaggcgttt acgtcttccg gtgcggcgga tactggacat catgcatcgt tcggaacttg
     4321 gcaagccgct gccggacgat ttcacgtttg agtttaaccc gctctggcaa atgtctgatg
     4381 tcgatcgctc aacggtggcg ttaaacacca ccaacgcaat cagtacggcg ctgggtgatg
     4441 gtctgatgac actgaaagcc gctatgactg atttgcgaga aaattctgac gtaaccggca
     4501 tcggggcatc cattaccgac gaggacatcg agaatgccga agatgaagcg ccgcccggca
     4561 tcggcgaacc tgatgacgaa ccgcaggagc cgtcaggcgg aaatccggta tcgaaccagc
     4621 ctacgcagga tagcgagggc ggtcggagac atcgtaaatg gtcgctacga tggttcaaat
     4681 gacagtatca cggaaattat tgcggcgctg gagcgttaca gtgaaatcat caccccctgg
     4741 gcgacaaagg tcgcggaaaa ctttactgcg gacctaaccc ggcagaacga gaaagtttgg
     4801 cggcaacaca gcaagaacat cagtcgcgag ctccgcaatc ttgtggaaag cgctcctgtg
     4861 ggccaggtga tgcaatccat catcgccgaa caggtcaagt acatcaaatc tctgcctctc
     4921 gaggccgcag acagggtgta cgacatccag aacaaagcga tagaggctgt tgtcaccggt
     4981 ggtcgggcgg agcagtttgc taaagagatt gcatctaccg gcgatgttgc taagtctagg
     5041 gccgatctga ttgcccgaac ggaactggga agagcaacgg gcgcgctgga tatggcccga
     5101 gcgatagcta ttggctcgga tggttatatt tggcgtacag ccgatgatgg cgatgtccga
     5161 gattcccacg atcacatgaa aggtaaattc gtccgctggg attcacctcc aactttggac
     5221 ggcatgaccg gccacgcggg cgagctgcca aactgccgct gctataaaga gatcgtgttt
     5281 gttcgcgttc cattcgcaat gaaaagggca gcataaccca tgaaatactt ttttgagacc
     5341 aggctcgggg aaacccgata ccgcctggct gacggctcgt tgctgtgcaa agacgtgccg
     5401 ataggacgaa caggtaagca gctctatggt gctgatgacc tgccaaaact gaaacccgat
     5461 aagttcggtg aaatagtcgt cacgcgttct cctgagcagg tattccatcc ggccacgctt
     5521 gcctcattcg aagggatgag catcacgatc ctgcatcctg aagatgaaaa cgggaatgtg
     5581 cggctggtaa atcccgagaa ctggaaagag cttgctgtcg ggcacctcca gaatgtccgg
     5641 cgcgggacgg gtgagcagtc tgatttgatg ctggctgacc ttatcgtcaa agacgaaagc
     5701 gccattcagc ttatcgaaga tggcctgcgc gaagtgtcgt gcggctatga cgcggagtac
     5761 gagcagaccg agccaggtaa agccgagcag gtcgatatta ccggaaacca tgtggctctt
     5821 gtccctaaag gcagagccgg aaatcgttgt gcaattggag acagagacac aatggcaaat
     5881 caaaagaaaa actggtggaa ccgcatgcgt gcggccatca agacaggaga tgccgacacc
     5941 atgaacgaac tggtggagtc ggctcccgca tcggttacag gagatgaggg ggatttgccg
     6001 cagggcgtta atctcaacat caacctgtcc ccgcagcaac cactaccgga caaagcacca
     6061 gagatgggcg gagatccaac cggcgacagt gatgatgacc tcaaaacatt actgaaagcc
     6121 ctgctggcta agctggaaag aaatgccacg ggcgataacg ataataagcc tgacgataat
     6181 ccgaccggtg acggcgagga cgatgaagag gaaaccacga ttactggtga ctcagcctgg
     6241 cgtgccgaag ttatcgttcc gggtatcgat ctgagccgta agatgaaacc gaccgcgttc
     6301 aaacgcgagg ttctggcttc tgctgacaaa acgctggttc gccagatagt cggtgatgcg
     6361 gatatccgca aattaccgaa acaatcggtc gacatggcgt ttaatgccgt gtctgaggtt
     6421 gccaaagggc gaaacacccg cgccaccacc ggcgatgcac agcgcctaaa catgggcatg
     6481 accagtatcg cttccctgaa caaacaaaac gctgaattct gggcaaaccg taaagggtaa
     6541 aaaatgaata atgtatttct gtaccggatg cctgttggca ttgccggggc tgtctctcgc
     6601 ccgcaggact taaccgtcga accggtggtc cttaaatccg ataacgcctt cgctgcctat
     6661 ggcctggctg gtaaatacga tgatgacggt tttttcgtgc cgctggcaga tggtgatacc
     6721 gcagacaagg tgaaggggat ctacgtgcgc ccttatccga ccacgtcgca gccggacatg
     6781 gttcgccagg tgggaacagg caagaacttc ccgggcgacg ccatgaagcg tggctacgtg
     6841 accgttaatc tcggttctga ttttgatgcc agcactatca aaaaaggcga tccggtatac
     6901 gttgtcgtct ccactgatga atccatcaaa gtgccgctgg gtggattcat gtccacgtca
     6961 gtcagtggca aaaacgtggt gctgaccaac gctgaattca caggtgccgg tgatgctgac
     7021 ggcaatgcag aaatttcctg gaagattta
//
LOCUS       DAJTYZ010000178         6988 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000178 DAJTYZ010000000
VERSION     DAJTYZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.178"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..645
                     /locus_tag="OO596_003734"
     CDS             <1..645
                     /locus_tag="OO596_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6719488.1"
                     /translation="PHAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICL
                     SLADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASG
                     MRGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDR
                     QLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            896..2458
                     /gene="hpaB"
                     /locus_tag="OO596_003735"
     CDS             896..2458
                     /gene="hpaB"
                     /locus_tag="OO596_003735"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR6719489.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2476..2988
                     /locus_tag="OO596_003736"
     CDS             2476..2988
                     /locus_tag="OO596_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6719490.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3381..5531
                     /gene="btsT"
                     /locus_tag="OO596_003737"
     CDS             3381..5531
                     /gene="btsT"
                     /locus_tag="OO596_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6719491.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5581..5784
                     /locus_tag="OO596_003738"
     CDS             5581..5784
                     /locus_tag="OO596_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6719492.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5795..6751
                     /gene="yjiA"
                     /locus_tag="OO596_003739"
     CDS             5795..6751
                     /gene="yjiA"
                     /locus_tag="OO596_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6719493.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
BASE COUNT         1611 a         1906 c         1850 g         1621 t
ORIGIN      
        1 cctcatgcgt ttattacgga gtctgacgcc gacggtcatg tgttgacggt acgggaagat
       61 ctgatctggc ccctgctgga agttctttat ccaggcactc gggaaacctt cggcctgccg
      121 gggatttgcc tgtcactggc agataaaccc gacgaactgg cggcgctgga acactattgg
      181 caactgatag agcgggaatc ggtggaacaa ctgcctggac gggaacacac cctgacgcta
      241 ctggcacagg cagtgttcac cctactgctg cgtaatgcaa aactcgacga ccatgccgcc
      301 agcgggatgc gcggagaatt aaaactgttc cagcgtttta atatgctgat tgaaagccat
      361 tttcatcagc actggacagt accggattac gctaacgaac tgcatatcac cgaatcacgc
      421 ctcacggaca tctgccgccg ctttgccaac cgtccgccaa aacggttgat ttttgacagg
      481 cagctgcgag aagccaagcg gctgctgctg ttttctgata acgccgtgaa caatattgcc
      541 tggcaactcg gttttaagga tccagcttat tttgcgcgct tttttaatcg cttagtcggt
      601 tgctcgccca gtgcttatcg tgccaaaaaa gtacctgtga cgtgaaaatc ccttagcctt
      661 aacgggaaac caggcaccac ctgctattcc cctttctccg cctggagcaa ggggatttag
      721 taacgtcaaa aatcgctaaa agcgaccgcg atcacaaaac cgtacccgac ctgaaaagta
      781 ccagccattg tcccaaaagt gtcttcccgt aatcgcccct gcaagcttca attgaataac
      841 aaaaaaaaca cacaaaatta acaatcgtat tccgattaat actgtagagg tcgacatgaa
      901 accagaagat ttccgcgcca gtacccaacg tccgttcacc ggggaagagt atctgaaaag
      961 cctgcaggat ggtcgcgaga tctatatcta tggcgagcga gtgaaagacg tcactactca
     1021 tccggcattt cgtaatgcgg cagcgtctgt tgcccaactg tacgacgcgc tgcacaaacc
     1081 ggagatgcag gactctctgt gctggaacac cgacaccggc agcggcggct atacccataa
     1141 attcttccgc gtggcgaaaa gtgccgacga cctgcgccag caacgtgacg ccatcgctga
     1201 gtggtcacgc ctgagctatg gctggatggg ccgtacccca gactacaaag ctgctttcgg
     1261 ttgcgcactg ggcgcgaatc cgggctttta cggtcagttc gagcagaacg cccgtaactg
     1321 gtacacccgt attcaggaaa ctggcctcta ctttaaccac gcgattgtta acccaccgat
     1381 cgatcgtcat ttgccgaccg ataaagtgaa agacgtttac atcaagctgg aaaaagagac
     1441 tgacgccggg attatcgtta gcggtgcgaa agtggttgcc accaactcgg cgctgactca
     1501 ctacaacatg attggcttcg gctcggcaca agtaatgggc gaaaacccgg acttcgcact
     1561 gatgttcgtt gcgccaatgg atgccaatgg cgtcaaatta atctcccgcg cctcttatga
     1621 gatggtcgcg ggtgctaccg gctcgccgta cgactacccg ctctccagcc gcttcgatga
     1681 gaacgatgcg attctggtga tggataacgt gctgatccca tgggaaaacg tactgatcta
     1741 ccgcgatttc gatcgctgcc gtcgctggac aatggaaggc ggttttgccc gtatgtatcc
     1801 gctgcaagcc tgtgtgcgcc tggcagtgaa actcgacttc attacagcac tgctgaaaaa
     1861 atcactcgaa tgtaccggca ccctggagtt ccgtggtgta caggccgatc tcggtgaagt
     1921 ggtggcgtgg cgcaacacct tctgggcatt gagtgactcg atgtgttctg aagcgacgcc
     1981 gtgggtcaac ggggcttatt taccggatca tgccacactg caaacctatc gcgtactggc
     2041 accaatggcc tacgcgaaga tcaaaaacat tatcgaacgc aacgttacca gtggcctgat
     2101 ctatctccct tccagtgccc gtgacctgaa caatccgcag atcgaccagt atctggcgaa
     2161 gtatgtgcgc ggttcgaatg gtatggatca cgtccagcgc atcaagatcc tcaaactgat
     2221 gtgggacgcc attggcagcg agtttggtgg tcgccacgaa ctgtatgaaa tcaactactc
     2281 cggtagccag gatgagattc gcctacagtg tctgcgccag gcacaaagct ccggcaatat
     2341 ggacaagatg atggcgatgg ttgatcgctg cctgtcggaa tacgaccaga acggctggac
     2401 tgtgccgcac ttgcacaaca acgacgatat caacatgctg gataagctgc tgaaataacg
     2461 cagcaggagg ttaagatgca attagatgaa cagcgcctgc gctttcgtga cgcgatggcc
     2521 agcctgtcgg cagcggtaaa tattatcacc accgagggcg acgccggaca atgcgggatt
     2581 acggcaacgg ccgtctgctc ggtcacggat acaccaccat cgctgatggt gtgcattaac
     2641 gccaacagtg cgatgaaccc ggtttttcag ggcaacggta agttgtgcgt caacgtcctc
     2701 aaccatgagc aggaactgat ggcacgccac ttcgcgggca tgacaggcat ggcgatggaa
     2761 gagcgtttta gcctctcatg ctggcaaaaa ggtccgctgg cgcagccggt gctgaaaggt
     2821 tcgctggcca gtcttgaagg tgagatccgc gatgtgcagg caattggcac acatctggtg
     2881 tatctggtgg agattaaaaa catcatcctc agtgcagaag gtcacggact tatctacttt
     2941 aaacgccgtt tccatccggt gatgctggaa atggaagctg cgatttaagc tccagcccct
     3001 tcacgcattc gtcgaagggg catttttatt gtgatctcaa tcacatatcc acccgatgaa
     3061 taattcttgc ccaaccgctc accgccattc ctgaccgctt acgtccctaa aataaccact
     3121 cagttattta ccttacttta cgcgcgcgta actctggcaa catcactgca ggatagcggt
     3181 caatttacct cctcaaacgc aacgcaaacc tagaacggct tcggcctatg attaacaaat
     3241 tactctatga attttggttt gcaggaaggc agcaagtgag tgaatccccg ggagcttaca
     3301 acagtaagtg acaggggtga acgaacgcaa ctgccgcacc tgtaagccaa aagacgacga
     3361 gtaaaatgcc aggttttact atggatacga aaaaactatt caagcacata ccctgggtga
     3421 ttctcggaat catcggtgca ttctgcctcg cggtagttgc attacgtcgg ggggagcacg
     3481 tcagcgccct gtggatcgtg gtcgcctctg tgtcggtgta tctggtggcg tatcgctact
     3541 acagtctgta catcgcccag aaggtgatga aactcgaccc cacgcgcgcg acgcctgcgg
     3601 ttattaacaa cgacggtctg aactacgttc cgaccaaccg ttacgtgttg tttggtcacc
     3661 acttcgccgc tatcgccggt gctggtccgc tggtcggtcc ggttctcgcc gcacagatgg
     3721 gccacctgcc tggcacgctg tggctgctgg cgggggtagt actggccggt gcggttcagg
     3781 actttatggt gctgtttatc tcctctcgcc gtaacggcgc atctctcggt gagatgataa
     3841 aagaagagat gggaccagta ccggggacta tcgcgctgtt tggctgtttc ttaatcatga
     3901 tcatcatcct tgccgtcctg gcgctgattg tggttaaagc cctggccgaa agtccgtggg
     3961 gtgtcttcac cgtttgctca accgtaccga ttgcgctgtt tatgggtatc tacatgcgct
     4021 ttatccgtcc ggggcgtgtg ggtgaagtct ctgtcattgg tatcgtgctg ctggttgcct
     4081 ctatctactt cggtggcgtg attgctcacg atccgtactg gggtccggca ctgaccttta
     4141 aagacaccac cattaccttc gcgctgattg gctatgcgtt tgtttccgca ttgctgccgg
     4201 tgtggctgat cctcgcaccg cgcgactatc tggcaacctt cctgaaaatc ggcgttatcg
     4261 tcggcctggc gctgggtatc gtggtgctga acccggaact gaaaatgcct gcgatgaccc
     4321 agtacattga cggtactggc ccgctgtgga aaggtgctct gttcccgttc ttgttcatta
     4381 ccatcgcctg tggtgcggta tctggcttcc acgcactgat ctcttccggt acgacgccga
     4441 aactgctggc taatgaaacc gacgcgcgtt tcattggcta cggcgcaatg ctgatggagt
     4501 ccttcgtggc gattatggcg ctggttgctg cgtccatcat cgaaccgggt ctttacttcg
     4561 cgatgaacac cccgcctgct ggccttggca tcaccatgcc taacctgcat gaaatgggtg
     4621 gcgagaacgc gccgatcatc atggcgcagc tgaaagacgt taccgcacac gcggcagcga
     4681 ccgtcagctc ttggggcttc gtgatttcgc cagagcagat cctgcaaacc gcgaaagaca
     4741 ttggtgagcc ttctgtcctg aaccgtgcag gtggtgcgcc aacgctggcg gtaggtatcg
     4801 ctcacgtgtt ccacaaagtg ctgccgatgg ctgacatggg cttctggtat cacttcggta
     4861 ttctgttcga agccctgttc atcctgaccg cgctggatgc gggtacccgt tctggccgtt
     4921 ttatgctgca agacctgctg ggtaacttca tcccgttcct gaaaaaaacc gactctctgg
     4981 ttgccggtat catcggtact gcgggctgtg tgggtctgtg gggctacctg ctgtatcagg
     5041 gcgtggtcga tccgctgggc ggcgttaaga gcctgtggcc gctgttcggt atctccaacc
     5101 agatgctggc agccgtagcg ctggtactgg gcaccgttgt gctgattaag atgaagcgca
     5161 cccaatacat ctgggtaact gttgttccgg ctgtatggct gcttatctgc accacctggg
     5221 cgctgggtct gaaactgttc agcaccaacc cgcagatgga aggcttcttc tacatggcaa
     5281 gccagtacaa agagaagatt gctaacggta ctgacctgac ggcgcagcag attgccaaca
     5341 tgaaccacat cgttgtgaac aactacacca acgcaggcct gagtattctg ttcctgattg
     5401 tggtgtacag catcatcttc tacggtttca aaacctggct tgcggtgcgt aacagcgaca
     5461 aacgtactga caaagaaact ccgtacgttc caatcccgga aggcggcgtg aagatctctt
     5521 cgcaccacta accgtgttta gccccgcttc ggcggggctt tgttcttatc agagtgaact
     5581 atgtttggta acttaggaca ggcaaaaaaa tatctcggcc aggcggcgaa gatgttgatt
     5641 ggcattccag actacgacaa ctacgtcgag catatgaaga ccaaccatcc cgacaagccg
     5701 tacatgagct atgaagaatt cttccgcgaa cgccagaacg cgcgctacgg cggcgatggt
     5761 aaaggcggta tgcgctgttg ttaatggaga gagcatgaac ccgattgcag ttaccctact
     5821 caccggtttt ttaggcgcgg gcaaaaccac cctgctgcgc catattctta acgaacaaca
     5881 cggctacgag attgccgtga ttgaaaacga attcggcgaa gtctccgttg atgatcaact
     5941 gattggcgac cgcgccacgc agatcaaaac gctgaccaac ggctgcatct gctgttcgcg
     6001 ctccaacgag ctggaagacg cactactcga cctgctggat aatctcgaca agggcaatat
     6061 tcagttcgac cgtctggtca ttgaatgcac cggcatggcc gatcccggcc cgattattca
     6121 gaccttcttc tcccatgaga ttttatgcca gcgctacctg ctggacgggg tgattgcgct
     6181 ggtggatgcg gtacatgccg atgagcagat gaaccagttc accatcgccc agtcccaggt
     6241 tggctacgcc gaccgtattc tgctgacgaa aaccgacgtc gcaggcgaag cagagaaact
     6301 gcgtgaacgc ctggcgcgca tcaacgcccg cgcaccggtc tacaccgtta cccacggcga
     6361 tatcgacctg ggccttctgt tcaacaccaa cggttttatg ctggaagaaa acgtcgtcag
     6421 caccaaaccg cgtttccact ttatcgcgga caaacaaaac gatatttcgt cgattgtggt
     6481 ggaactggat tacccggtag atatcagcga agtttcccgg gtgatggaaa acctgctgct
     6541 ggaatcggcg gataaactgc tgcgttacaa agggatgctg tggattgacg gcgaacctaa
     6601 ccgcctgctg ttccagggcg tccagcgcct ctacagcgcc gactgggacc gcccgtgggg
     6661 cgatgaaaaa ccgcatagca cgatggtgtt tatcgggatt cagttgccgg aggatgagat
     6721 tcgggcggcg tttgcgggac tcaaaaagta accagccctc caacatcaca atacctgaga
     6781 ctttatcacc ctcatattcc tccagaatat gagggcattc gggcttaatt atctctgaac
     6841 gtgcaacact tcctgcgact ccttatgcgc ctccacatcc tgcttcgtta accagagcga
     6901 cttacgacca aaattttcgt acattttcag ctgatcttca tagtgctgat caactgttcc
     6961 atcgggagca ataaacccac tctgaccg
//
LOCUS       DAJTYZ010000179         6907 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000179 DAJTYZ010000000
VERSION     DAJTYZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.179"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(308..889)
                     /locus_tag="OO596_003740"
     CDS             complement(308..889)
                     /locus_tag="OO596_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719494.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(936..4793)
                     /gene="sigA"
                     /locus_tag="OO596_003741"
     CDS             complement(936..4793)
                     /gene="sigA"
                     /locus_tag="OO596_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6719495.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5154..6158)
                     /locus_tag="OO596_003742"
                     /pseudo
     CDS             complement(5154..6158)
                     /locus_tag="OO596_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6143..6520
                     /locus_tag="OO596_003743"
                     /pseudo
     CDS             6143..6520
                     /locus_tag="OO596_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6559..>6907)
                     /locus_tag="OO596_003744"
     CDS             complement(6559..>6907)
                     /locus_tag="OO596_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6719496.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1723 a         1531 c         1559 g         2094 t
ORIGIN      
        1 taccaatact ggcttaatgg ctacatacgg cagtcggttt acgcttacct ccgttaacgc
       61 ttacctccgt taacgcttac ctttaatctg ctgaatttcg aattgttcag gagtaatagt
      121 ccaaagtatc gggaattttc cagacaatcc tgataatacg cttatgaaca ttacaacacg
      181 ctcatgcgga acctggatgg gggaaggaac ggtgaagctg ttagcgaatg accggcgttt
      241 gtataaacgc cggtcatgat gaatgatatt tcagaatata cctcgggcta aaataccggg
      301 acagagatca ggcgtgatta atctgcagca tttttaataa cagaagtact gagcttactt
      361 tacctttgat gatgtaatag ttatttccgt cggtactcat ttttccctga aataatgtca
      421 cggatgtttc ttcctgctga tcctgcaggc gcagtcgtaa ggtcatattt ttaatgagct
      481 gagatccggg tggcaagggg tatttgctgt cagatactgg gcgactgagt ctgatgtggc
      541 gcgtatacag cccgttttcg tacagggtgg cctctgttat ggtgtcagtg accagcatgt
      601 gggagacaat gctgtattct tttttaaagc actcaccaga aacggtgatt gtaatgataa
      661 tggtcttctc gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg
      721 agaactcagt tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt
      781 ctttttttat tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa
      841 agctaataat gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctacgg
      901 tagaagaagg gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg
      961 ttgatcgcgt tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag
     1021 cgaagattat cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt
     1081 tcacctttga tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac
     1141 aggtcaaact ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag
     1201 gatttaccca catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg
     1261 gtcacgctca ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc
     1321 agttctgcct gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca
     1381 gcacccacat acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc
     1441 gagtactcat tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca
     1501 aatatagcgg aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta
     1561 ctgctggcat gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc
     1621 agctcatgtt ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacta
     1681 gaagctgaac ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca
     1741 cgcagatccc ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta
     1801 ccggacatat acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg
     1861 gtcagggtcc acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg
     1921 ctgaaaccta tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct
     1981 gttatcaggt caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac
     2041 agactgttat tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca
     2101 gactggctca gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact
     2161 gtcacagtat ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt
     2221 tttaatgatt gcagggctga ctgtccgttt actctccata aggtgttata catgctggct
     2281 gaggcagcag gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct
     2341 gtagccagcg gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca
     2401 aacagaatag atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga
     2461 gcctttaacg tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg
     2521 ctgtatttat tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata
     2581 gctttatcta catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg
     2641 tctgtattaa ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt
     2701 attttgctgt tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg
     2761 gagcgaagct gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg
     2821 tcaatgtatg cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct
     2881 tttacattag catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa
     2941 ttaaatttac gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta
     3001 tattccgtat tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag
     3061 tctttttgac ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt
     3121 tctgtcgcat gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata
     3181 tcgactgagc catcaaagat cagattggca ttattgttat gttgctgggt agtattgata
     3241 atatgattta tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta
     3301 gtaagtgata atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt
     3361 gctgcgattt tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg
     3421 gtaaaataga tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc
     3481 gtacctttac ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca
     3541 gtcccattac ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta
     3601 cctattttat gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta
     3661 ttatcaatat ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc
     3721 tggttcttat cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat
     3781 tcccctcctc ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt
     3841 ttaccatcca gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta
     3901 ttaaataacg agtagtttgt ccaggctata ttggtactac tgagaaaacc aactgtactg
     3961 gtaacaccat ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca
     4021 ctatcgccaa aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg
     4081 tttttccagt ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca
     4141 aacagatatt gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca
     4201 attatttgtt ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa
     4261 agtgagaagt cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga
     4321 tgaaccgaaa agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat
     4381 gactgtgttg ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca
     4441 cctccaattg atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt
     4501 tctggaaaat gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt
     4561 ctgaactctc ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata
     4621 tccagcattg ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata
     4681 ccgaggataa ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca
     4741 gcaactaatc cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa
     4801 ctccgatatt tctgagttgt ggttatggga tagcatttaa ttacaacggg gaactatcag
     4861 tcatggtacg taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca
     4921 gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggtcaga tattgatcac
     4981 ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt ccggtcaatc
     5041 ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt
     5101 gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag
     5161 gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca
     5221 tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg
     5281 ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat
     5341 tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc
     5401 agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc
     5461 tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg
     5521 gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca
     5581 ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg
     5641 ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg
     5701 gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc
     5761 acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc
     5821 tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta
     5881 ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg
     5941 ggaagggccg ccacagcaga aactcccagc tgtgcggcgg catgtgtgtt acggaagtcc
     6001 cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg
     6061 cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga
     6121 ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat
     6181 ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg
     6241 tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt
     6301 caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga
     6361 acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg
     6421 ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga
     6481 aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt
     6541 gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag
     6601 tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg ctacttgtga
     6661 ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca
     6721 tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc
     6781 aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc tcagttcgct
     6841 gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg
     6901 caccagt
//
LOCUS       DAJTYZ010000180         6858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000180 DAJTYZ010000000
VERSION     DAJTYZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.180"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..123
                     /locus_tag="OO596_003745"
     CDS             <1..123
                     /locus_tag="OO596_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6719497.1"
                     /translation="CTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            127..1482
                     /locus_tag="OO596_003746"
     CDS             127..1482
                     /locus_tag="OO596_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6719498.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            1530..2570
                     /gene="gatD"
                     /locus_tag="OO596_003747"
     CDS             1530..2570
                     /gene="gatD"
                     /locus_tag="OO596_003747"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6719499.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            2676..3449
                     /locus_tag="OO596_003748"
     CDS             2676..3449
                     /locus_tag="OO596_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6719500.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(3531..4430)
                     /gene="yegS"
                     /locus_tag="OO596_003749"
     CDS             complement(3531..4430)
                     /gene="yegS"
                     /locus_tag="OO596_003749"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6719501.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            4836..5153
                     /locus_tag="OO596_003750"
     CDS             4836..5153
                     /locus_tag="OO596_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719502.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(5483..6844)
                     /gene="yegQ"
                     /locus_tag="OO596_003751"
     CDS             complement(5483..6844)
                     /gene="yegQ"
                     /locus_tag="OO596_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6719503.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
BASE COUNT         1698 a         1549 c         1767 g         1844 t
ORIGIN      
        1 tgcaccactg ccaaagtgga tcgtagtttt ggcgatattc cgttagttca cggcatgcct
       61 tttatttctg gtgtcggtat cgaagcatta caaaataaaa ttctgactat cttacagggg
      121 tgacctatgt tttcagaagt catgcgttat attctcgacc tcggccctac ggtgatgctt
      181 ccgattgtca tcattatttt ttctaaaata ttaggcatga aggcaggcga ttgctttaaa
      241 gcgggtctgc atatcgggat tggctttgtt ggcattggcc ttgtgattgg cttaatgctg
      301 gattccattg gtccggcggc gaaagcgatg gcggaaaatt tcgacctgaa tctgcatgtg
      361 gtcgatgtcg gctggccggg ctcttcacca atgacctggg cgtcgcaaat tgcgctggtg
      421 gcgattccga ttgcgattct ggttaacgtg gcgatgttac tgacccgtat gacgcgggtg
      481 gtaaatgttg atatctggaa tatctggcat atgaccttca ccggcgcgtt gctgcatctg
      541 gcaaccggtt catggatgat agggatggca ggtgtggtaa ttcacgcggc gtttgtttat
      601 aagctcggcg actggtttgc ccgcgatacc cgaaatttct ttgagctgga aggtattgct
      661 attccgcacg gtacgtcggc gtatatgggg ccgattgcgg tgctggtcga tgctatcatc
      721 gagaaaatcc caggcgttaa ccgaattaaa tttagcgccg acgatattca gcgcaaattt
      781 ggtccatttg gcgagcctgt caccgtgggt tttgtgatgg ggctgattat cggcatcctc
      841 gccggttacg atgtcaaagg tgtattgcag ctggcggtaa aaacggcggc agttatgctg
      901 ctgatgccac gggtgattaa acccatcatg gatggtttaa cgcccatcgc taagcaggcc
      961 cgtagtcgtt tacaggcgaa gttcggcggt caggagttcc tgattggcct ggatcccgca
     1021 ttactgctgg ggcatacggc ggtggtatcg gcaagcctga tttttatccc gctcaccatt
     1081 ttaattgctg tttgtgttcc gggtaatcag gtactgccgt ttggcgatct tgccaccatc
     1141 ggcttcttcg tggcgatggc ggtcgcggtg catcgtggaa atctgttccg caccttaatc
     1201 tcgggtgtca tcattatgag catcaccctg tggatcgcga cgcaaactat tggtttgcac
     1261 acccaactgg cggctaatgc tggggcgtta aaagccgggg gtatggtggc ttcaatggat
     1321 cagggcggtt ctcccattac ctggttactg attcaggttt tctccacgca aaatattccc
     1381 ggtttcatta ttatcggcgc aatttatctt accggtattt tcatgacctg gcgtagagcg
     1441 cgtggtttta ttaaacaaga gaaaaccgtt ctcgcagaat aatttttacc tgagggggag
     1501 gttatccccc ttaacaatca ggagttttta tgaaatcaat ggtgaatgat actgatggta
     1561 tcgtgcgcgt tgcagaaagc gtcattcctg aaattaaaca tcaggatgag gtgcgggtaa
     1621 aaattgccag ctcgggctta tgtggttccg atttacccag gatatttaaa aatggtgcac
     1681 attattatcc aataacgtta ggccatgaat ttagcggcta tattgatgcg gtgggatccg
     1741 gtgttgatga tttacaccct ggcgatgcgg ttgcctgtgt gccgttatta ccctgtttta
     1801 cttatccaga gtgtttgaaa gggttttatt cccagtgcgc aaaatatgat tttattggct
     1861 cgcggcgtga tggtggattt gctgaatata ttgtcgttaa gcgaaaaaat gtctttgctc
     1921 tacccacgga tatgcctatt gaggatgggg cttttattga gccgattacc gttggcctgc
     1981 atgcttttca tttagcgcaa ggttgtgaga ataaaaacgt tattattatt ggtgccggaa
     2041 ccattggcct gctggctatt cagtgcgctg tcgcgctggg agcaaagagt gtgacggcga
     2101 tcgacattag ctcagaaaaa ctggcattgg caaaatcttt cggtgcgatg caaacattta
     2161 acagtagcga aatgagcgcg ccgcaaatgc agagcgtttt acgcgaactg cgctttaatc
     2221 agcttatcct cgagacggct ggcgtaccgc aaactgtcga actggcggta gagattgccg
     2281 gtcctcatgc ccaactggcg ctggtgggca cgttgcatca ggatctgcat ttaacatcgg
     2341 caacgtttgg caaaatattg cgtaaagagc tgacggttat cggcagttgg atgaactatt
     2401 ccagcccttg gccggggcag gagtgggaaa cggcgagccg gttgctgaca gaacgtaagt
     2461 taagcctgga gccattaatc gctcaccgtg gaagctttga aagcttcacc caggcggtgc
     2521 gtgacatcgc tcgtaatgcc atgccgggca aagtgttgct cattccatga aaccgcgggc
     2581 cagcgtgatg ctggcccggt attgtgcaaa acagattatt caccaatggt cccccttcgt
     2641 ttacactagc cacaattgaa tatgggtaaa tgacgatgaa ttcattcgag cgaaggaata
     2701 agatcatcca attagtgaat gaacagggaa ccgtgcttgt tcaggatctg gcgggagtat
     2761 ttgctgcctc ggaagcgaca atccgtgccg atttgcgctt tctcgaacaa aaaggcgtgg
     2821 ttacgcgctt tcatggcggt gcggcgaaaa taatgtccgg taatagtgaa accgagaccc
     2881 aggaagtcgg gtttaaagag cgatttcagc tcgccagcgc gccaaaaaac agaatagcgc
     2941 aggcggcagt caaaatgatc catgaaggga tgaccgttat tctcgacagc ggaagtacga
     3001 caatgcttat cgccgaagga ttaatgaccg ctaaaaatat cacggtgatt accaacagtc
     3061 tcccggcagc gtttgccctt tccgaaaata aagacattac tctggtcgtc tgtggtggca
     3121 cggtccgcca taaaacgcgc tcgatgcatg gttccattgc cgagcgttca ttgcaggata
     3181 ttaatgccga tttaatgttt gtcggtgctg atggtattga tgcggttaat ggtattacga
     3241 cctttaatga aggttattcg attagcggtg cgatggtcac cgccgctaat aaagtgattg
     3301 ccgttctcga ttcatcgaaa tttaaccgtc gcggttttaa tcaggtactg ccaatcgaaa
     3361 aaattgacat tattattacc gatgatgctg tatcagaggt ggataaactg gcattgcaga
     3421 agacacgggt aaaattaatt acggtgtagt attgttctgt atgccggata aggtatttac
     3481 accgcatccg gcatttcgga ttctccctgg ttcaagagag aatagtttga ttaacgcaat
     3541 aatagacaat ctggtggtaa tcgacaacgc aacgccgccg gaagtatttc aatatgaaag
     3601 ttttgtccgc tcaacggttc gccatcaaga ttaaaagtga tttcgtgtgg ggcttgtata
     3661 tcaaaccacg acgaagcgcc ttcgataata ttcgggttat cttcgtcaga ttttaaggtt
     3721 gatacgagag ccggaagaat ttcatcgccg gtaaaaatgc gcagttgcag caagccatcg
     3781 ttaattaacg cgttcggaca caattgctga ccgccaccgg cctgacgccc gttaccaata
     3841 ccaatgacca gggcgtcacc ttgccagtga aagttttcac cgcggatttc acaacggtcc
     3901 ggttgcagag tatccatgcg cattaagcca tgaatgatgt aagagacgcc acccagcgcg
     3961 gcttttaatt tttccggcgt ttctgtggta atacgcgtcc caaatccgcc tgtcgccata
     4021 ttaataaaac aggtttgttt gttgacctgc gccatatcta tcgcaatggc gttaccggca
     4081 attgccagtt tcagcgcctt atccagtgcc tcaggaatcc ctacactggt ggcaaaatca
     4141 ttggcggttc ctaatggcaa aattcccagc gcgggtatgc catccccctc acactgaatc
     4201 aacgccgtag aaacttcatt aatggtgcca tcgccaccac cggcaatcac cgttgcgacg
     4261 cccaacttcc gggcctcctc tacatatcgt gcggcatcgc ctttctccca ggtgacccgc
     4321 acatggatcg tcattccttc ctcacgcaac agcataattg cttcgcgcaa gggtagattg
     4381 tcagtacttt tgccattaag aatcagtaag ctggcgggaa attctgccat gatcgtatgt
     4441 gcctttatga ttggtctgta gatagtgtag agcagaaaac aaaaggtgga gtcagaacag
     4501 gatgaaagtc ggaggataaa aagtcagttc aagtatggag gattcagtgg ttaccaccag
     4561 gagcttgcaa tggggacagc gaatactaac ggctgccacc gataaatttc aaaaaagagc
     4621 atatacctaa tattcaacta aacagtggca tcttcaatat aatatattaa agcccccatg
     4681 gagttaccct gaagggcctc aatgtccgta attcctactt atgtaggaaa tgttgtacag
     4741 aacatttatt ataatcctat tcaattataa taatcatgcc attattatat ttaaacacta
     4801 gagagtgtcg ttggtattta atgggggaag gtgagatgaa aaagatagct gctatatcat
     4861 taattagtat ttttcttata tctggttgtg ctgtgcataa tgatgaaaca agtatcggta
     4921 aatttggtct tgcatataaa agtaatattc agcgtaaact cgataaccaa tactacaccg
     4981 aagccgaagc ttctttagct aggggaagaa tatctggtgc agaaaatata gtaaaaaatg
     5041 atgcagccca tttctgtgtt actcagggca aaaaaatgca gatagttgac ctgaagacag
     5101 aaggtgcagg attacatggc gtcgctcgtc tgacattcaa atgtggagag tgagaatatt
     5161 ttttggtaag catcaaacat gcgcgttctg gttgtgctta gccggaacct gtgcgagcac
     5221 gatgccgtta cgtgaaaggc atcgtgctat gaagggagat tctatcgatg tggtcaatgg
     5281 aagacggtga ccagggatag ggcttatgca taaaaaataa gcccgtgtaa gggagattac
     5341 acaggctaag gaggtggttc ctggtacagc tagcatttta tgggttatgt ttttcagcga
     5401 aacggatgat aaccttaata aatgcagctg tatgtgatcg gtttctaaga attttccatc
     5461 cgggaaaaat aatcgaaatt aatcacttac cgtgtgggtt acgcgtggtt tccccggaga
     5521 aattacgcat cagcagcgcg taatttagct caagatcctg cgggaccggg agccacacag
     5581 tataaccatc gcctggtgcg accggcatcg cttcaccttt ggcgttttcc atgtgctcaa
     5641 gggtaaagtt aatgttgcct tgcggcgtca tcagctcaag gctgtcgcca acggagaatt
     5701 tatttttcac cgctaccgct gcgaggtccc ccttgcgctc accggtaaac tcaccaacaa
     5761 actgctggcg gtcagaaact gaataaccgt attcgtagtt ctgataatcg tcgtgagtat
     5821 gacgacgcag gaaaccttcg gtatagccac gatgcgccag accttccaga gtttccagca
     5881 ggctggtatc gaacggtttg cccgcagcgg catcatcgat agctttacga taaacctgtg
     5941 cggtgcgtgc gcaatagtag aaagatttag tacggccttc gattttcagc gaatgcacgc
     6001 ccattttggt caggcgttct acatgggcga tggcgcgcag atctttcgag ttcatgatgt
     6061 aagtgccgtg ctcatcttca aacgcggtca tatactcgcc cggacgctgg gcttcttcga
     6121 tcataaacac tttgtcggtt ggcgcgccga tacctagcgt cggctcaaca ttttgcaccg
     6181 gaatcggctc gtacttgtgt acgatgttgc caacgtcatc ttctttccct tcctggacgt
     6241 tgtactccca gcggcaggcg ttggtgcagg taccctggtt cggatcgcgc ttgttgatat
     6301 agccagagag caggcagcga ccggagtagg ccatgcacag cgcaccgtga acgaagatct
     6361 caatctccat atccggcacc tgattgcgga tctcttcaat ctcttccagc gacagctcgc
     6421 gagagaggat cacgcgggtc aggcccattt gctgccagaa tttcaccgtc gcccagttta
     6481 cggcgttagc ctgcaccgag aggtggatcg gcatttcagg gaagtgctca cgcaccagca
     6541 taatcagccc tggatcggac ataatcagcg catccggccc catttccacc accggtttca
     6601 ggtcacggat aaaggttttc agcttggcgt tgtgcggtgc aatgttgacc acgacataaa
     6661 actttttccc cagcgcgtgg gcttcattga tgccgagctg aagattttcg tggttgaatt
     6721 cgttgttgcg cacacgcagg gagtaacgcg gctggcccgc ataaacagca tctgcgccat
     6781 aagcgaaagc gtaacgcata tttttcagcg ttcccgccgg ggaaaggagt tccggtttaa
     6841 acataattct ctcgttct
//
LOCUS       DAJTYZ010000181         6762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000181 DAJTYZ010000000
VERSION     DAJTYZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.181"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(61..204)
                     /locus_tag="OO596_003752"
     CDS             complement(61..204)
                     /locus_tag="OO596_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HCR6719504.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGL
                     ATQ"
     gene            complement(302..553)
                     /locus_tag="OO596_003753"
     CDS             complement(302..553)
                     /locus_tag="OO596_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5 immunity protein"
                     /protein_id="HCR6719505.1"
                     /translation="MKLSPKAAIEVCNEAAKKGLWILGIDGGHWLNPGFRIDSSASWT
                     YDMPEEYKSKIPENNRLAIENIKDDIENGYTAFIITLKM"
     gene            complement(621..2291)
                     /locus_tag="OO596_003754"
     CDS             complement(621..2291)
                     /locus_tag="OO596_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HCR6719506.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALSTAMESRKKKEDNKRDAEGKLNDEL
                     AKNKGKIPDLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGR
                     NDPATVYGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
     gene            complement(2524..4743)
                     /locus_tag="OO596_003755"
     CDS             complement(2524..4743)
                     /locus_tag="OO596_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6719507.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            4922..5188
                     /locus_tag="OO596_003756"
     CDS             4922..5188
                     /locus_tag="OO596_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR6719508.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARIRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
     gene            complement(5402..6328)
                     /locus_tag="OO596_003757"
     CDS             complement(5402..6328)
                     /locus_tag="OO596_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6719509.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNVSLPV
                     PLSPAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRG
                     AVATSARTLKPWETLGISRAWYYQLKKRGLVE"
BASE COUNT         1597 a         1889 c         1547 g         1729 t
ORIGIN      
        1 aaaaaaaagc gcgtcagaat cagcctgaca cgctttgttc ttgtcaaaga ggatttcggg
       61 ttactgcgtt gctaatccgg tcagttcagc tgaggatgac ggggatacag tcccgccctg
      121 aacatcgcgg atatagtttg cctgacatgc agcaagaata atggctgcaa gaagcaataa
      181 aataatccct gttatttttt tcatactgtt ccttgtatat atggactcag gcattcatcc
      241 ataaaaacct gaaatccaaa ggttgaaatc atccatgaaa cataaaattc tataacacta
      301 tttacatctt taacgtgata atgaaagcag tgtatccatt ctcaatatca tctttaatat
      361 tttcaatagc caatctatta ttttcaggga tttttgattt gtattcctcc ggcatatcat
      421 atgtccatga tgctgaacta tctatcctga atccaggatt cagccaatgc ccaccatcaa
      481 tgcccaaaat ccataagcct tttttcgctg cttcattaca aacttctatt gcagcttttg
      541 gtgataactt catagaagca tttcctttta attcaaaaaa ggcatcacca ctatcatata
      601 acaccgtatg ttcaaataat ttattggtca tttttatttc cccattgaat tctcgaatcg
      661 tccacccaac ccggatctgt cttatcacta atctgagtaa cctcaccagt acgatcattg
      721 acaacaacat atttaccagg agaaccatac actgttgctg gatcatttcg acctaaatag
      781 tctggaggtg ttttcttcgg actacgctta tcaaaagatg taccagttgc accgttagat
      841 acggtatttt tgatatcatc ctctgtccat cctctttctg gcatttgtcc cctaattttc
      901 tgatctattt ttagatctgg tatctttcct ttattttttg ctaactcatc atttagtttg
      961 ccttcagcat ctcttttatt atcctctttc ttcttcctgc tttccatcgc agtacttaat
     1021 gcagcatcag catctgcctt ctcttttgca gcagcatcaa atgcagcctg cttattattt
     1081 acatccgtct gcgcccgctg ggctttaagc ccggccattt gccacattct gtgaccgcca
     1141 gccattgggt catgtgcaaa tcgttcaaat tgttttattt cagctattgc atcagcaaga
     1201 gttttattcg ctgcatcaag ttcgctttta cgcgaattat aaacctgaac agctttagcc
     1261 tgtcgctcct gatttctggc aacatcttca tttgcctgat tcagctctgc acgcgcgcgt
     1321 tcataatttc gctcagccgc ttcaaccgga tgcgtagcat cccattcctg ctgacggcga
     1381 ttttcttcat cctgacgttg ttttacctgg tcagggctaa gaacatcact cactgaaaca
     1441 tatacggcat tatgaccgct gtccttcggg aatcgaataa ctgcatccct ggtattaccc
     1501 ccctgagtaa atcctgccgg gcgaacatcc ttatcagtat tatttgtaac acctgggctt
     1561 aatgtctgta ctgctggcgt actgttatta actgaaatat tcagaacagg tgcacctgga
     1621 attgatgccg taaaaacacc tggacgttcg gtaggttttg catcaaccac cggaacactc
     1681 atcggaacac ctgaaacaac cgaaatattc tgtcgttcgt cttttacatc atcaacaaca
     1741 cgaacattta cgtttactgt tgccttatcg agaggtaatg aactgacagg tgattcagta
     1801 atatcatctg cgggtaatga cgtcacaatc tttgacatca tattggggtc atctttcgct
     1861 atttgtgatg gcaatacacc atataaagcc accccccaaa gaccaaattt aaacggtcct
     1921 ttcagggcag ccataatatc agcaatagct gccgataatg ctcccgctga aatactgacc
     1981 gccagaccgc cagcacctgg agtggaaagt gccggaaaac caaatgccac tggcgcagct
     2041 actgctgaca gattaccgcc tgttcccgag ccaccaccgg aattaccatt ccccccgcca
     2101 ttaccatgac cggaaccacc tccccagtga atgccgctac cggaaccacc accccacggg
     2161 ttattttccg aactccaccc ggaaccatca gaagcaccac cacctacacc aagcccggtc
     2221 gggccaccat taatgttacc acttgtgcta tgcgcgcccg tgttatggcc gcgtccatcg
     2281 ccaccgctca taaaaattcc ctcttttaag cgttaaaaca attaattaca cgattaatac
     2341 tgtacataaa accactggtt ttatgtacag atcaaaaagt acccgtcatc gttttttctg
     2401 tcaagctcag gtggaaaatt ttttagaaat caggagggat aacacttaga aaacgcagag
     2461 cgggccacaa aaaattttgt ggcccgtgat gtgttggcgg ggtccggggt gcagccccgg
     2521 acatcacata ctcatgcccc ggaagtgccc ggggcggcgt tgttgttgcc tgtgctgttc
     2581 gtcccgcttc cactcatgcc aggtctgatt aaaaccgagt tgacggaaga gataatccgg
     2641 catctcttcc ggtttgtgga cagcagccaa aagcctgtca tgatggcctt tgagccggaa
     2701 ctgcatcagg tgcggttccg gtgggtacgg agtatcctca tcatggttta tagcttctcc
     2761 gtaacctgcc aggcataccc cgttctccgt gatggtgctg actatcagat gactgtaatc
     2821 cggcgacgga agcacaagaa cggggcaaaa ccctccttcc cggcgaacaa acccgacaag
     2881 catttcctcc ggccctttgc ataacaacag attcagcgtc ctgctgtttc cctctctcga
     2941 aaatacaaca ggcatgagtt gttccggtac agcatcagcg gtgatatcca cccggtgcgc
     3001 tgacggcagc ggagaggcta cgggcctgcg gtcgtcgctg aaataaaaat tatccggcca
     3061 gtgatgcggc agcgccggaa aaccgtaaga gggccacccc tggcgcatct gttccagtac
     3121 atgccggacc gtcgcaccgg aatacattct cacacgctca ggccgcgcca tcgtctcatg
     3181 agattctggc attccctcac gtatatccga gaccatcaca gccccgcctg gcgacaggta
     3241 aacattcacc gggtcatgaa cactctgggg gtaatgcgcc agctcatcac cccataccgt
     3301 gaattctccg gctctgttac tcagcatcat gtagcgggtt atcctgcccg ttcccggctc
     3361 tctggcacgc cccagctccg taataacccc gactaatgcc gtttcacggc tcagacggtg
     3421 gttatcatga cgtttcagcc gcgcctcatg ctcctgttcc ttccggcgcg cttccgcttc
     3481 ggcttcctcc ctgcgccgct ccgcttcccg tgctgacagt ttttctttca gctctgccat
     3541 tcccggcgca atccacgtat tccactgcgt ttcaaaggcc tgacgacggg cgggaatgtc
     3601 ccgttctttc acggccctgt aggtgcggcg aagagattca atctgtttcc cgagtttcca
     3661 gaaacggagt tgtcttttgc ggtattccgg ttcagcccat gtcatcaggg ggatctctga
     3721 cagaagggaa gcgacagggc gtgctttttt gagtccggtc atctcctttt cgagaatggc
     3781 ctgctctttc agcagcgcat tcccctcacg ttcgatgtcg ttcaccttcc cgctcatcgc
     3841 caggaactcc ggggtcatct cccacgcgtt gccgcattcc tgcggcaacg tggcaatgaa
     3901 ttcccgcagg gtatccgctg aggcattcgc agcgggaatg gtcaggttaa gggcttccgc
     3961 tgcacgacgt gcggcatcgc tgagttcccg tttccgcacg ttctcctgta cctggatgac
     4021 tgccagcctt gtgcgggaaa cctgtatttc caggttaagg ctgttaatca gacggttctg
     4081 ctcaccgcgt tctgtttcca ttccccgttt ttccatcgca caggcagcct ttccgaggtg
     4141 aaggccgggc gctttctcca gtccctgacg ctcgtatgaa cgatggtcta tctcttcctg
     4201 gtagccggcg ttcgcaagcg ctctgttcgc atggtcagcc catgatgcgc gccacgtctc
     4261 ggcgagtgcc cggtcgttcc agtccctgac ctttcctgca aatcccgttt ccccgacagc
     4321 tctcgtggtc agcatgatgt gcgcatgggg attggtccgg tccatgtgat ggaacgccac
     4381 atcggcaatc atgccccgac tgacaaagtt ttcccggaca aaagcgagaa cggtctcccg
     4441 tgcggcctcg cgggaaatct cacggggaat ggcgagttca atctcccttg ccagctggga
     4501 gttccggcgc tgttcggctt tctcgaccgc attccagaga acggaacggt caccacacca
     4561 ggacggtgca ttcgcagggg tgagaatgac cgcatcatca acgccgcgtt tacgggtgta
     4621 atcgtgggtt aatccggtac ggtcatcggg tatgacagag ccggaacggt aggcagcaga
     4681 agcaacggca ctgtagccgt tttttcgcga aatgattttc atgctgagat gataaatcgc
     4741 catttctgcc gtaatgcctt tttgccgttt tcttttagcg tcgcagaccg cacacaatcg
     4801 aaacgaagtg gagattgtat aagtgcgcac ttcgtgatgt gccgtgcatt atgccagttc
     4861 agttttgcaa ctaccagtga cgcgctaaaa tgcgattttt caccgcgcat ggagtcacaa
     4921 aatgagcaga acactcgaac agaagattgc cgaagctgag gccagattac agcgcctgaa
     4981 ggcgaagagc cggagcctgg acacggcgca aaaagtcatt gtgggggctg ccatgctggc
     5041 gaggatacgg aggccggaag aggcgcaact gcgtgcgttc ctgctccagt tcctgcgaaa
     5101 ggaagtcaca agacaggccg atgtgaaccg gatacaaccg ctgattaacg agctggagaa
     5161 gctacccaaa ccacctgcga aaccgtagaa cggagaaacc ccggcgcgta agtgccacat
     5221 gcaccaccga cacctttgcg cttcctcgct cattcgctcg ctacgctcgg tcatgagact
     5281 gcggcgagcg ctacaacctc aatcatgcgg aaagccgcac cattgcaggg aggaaacgaa
     5341 aaagccacca aacgccaaca gctcaaaaaa gcgctgttat ctgatatagg ttttatttgg
     5401 tctactctac aagacctcgt tttttcagtt ggtaatacca ggcgcggcta attccaagag
     5461 tctcccacgg tttcaacgtt cgcgcagatg tggcaacagc accacgctta gacttagcac
     5521 ctccaatttt cccgcccttg cgaccacgtg cagcctgaat ttctggcgtg tgcgtatcag
     5581 ccacatactg tgcgaaagtt tccggcgtga agttcctgtg cgtgtatttc gcaatgctct
     5641 tgccaatagc ccgacattcc gccggagaaa gcggaacggg aagcgatacg ttgtacattt
     5701 cgacacgctg gatcacggca tcaagccatt gtgagaatac aggccagccc tgacgaatcg
     5761 ccctgtaagc ccatttgcgc gtcatttcga acaggtggca gtttcgcccc atcccgtaat
     5821 gtttatcgat gctacggcgc gctgaggcgc tcaaatcgag ataatcagcc agttcatcga
     5881 gggtataggc ttcctcgcgc cactccatca ccagccattc caggtggaac ggatttttgc
     5941 aaatcagtcc gctgtagttc acatctgcac caagtttctc acacagcgca cgctctatcg
     6001 cggcagcgta cttcagtgcc ttaaccgacg catcaggcgc ggttctcacg gcgatgttga
     6061 gtgcatagag caggtgagca tgaccgttca cgggattttt tacggtgatg ttcggcgcgg
     6121 gggcgttccg gtcgctccag tcaatcgcag ccccctcgcg gtcaacgtca aaaaccagcc
     6181 agaactgcgc atgaggctgg ttctgctgga tgtaccgcgc aagaagcgca cgccctttgc
     6241 cggagatgcg aagaccaaaa gcgagatcat ccgtgtgata gggacggtgg ggtaaatttt
     6301 cttcgaagta ttgaagcgcg gcgctcatgt gttacgattc cttccaggtt atcgaccgac
     6361 ctcgcccctg tattctcgtg acaatcttgg cggagagaca cgaatttcag gggcgtttgt
     6421 ttttcagtcg cttacactac acccaacggc aaatcctgtc gaaccttccc gaaaacccga
     6481 tccatttacc ataactcccg ttgtacgtac cccttaaaac gccctcaatc gctttctgac
     6541 gcaaaaaacg caaaaagaca aggaaacaca gggtaaacat aaaaaaatgc gtcagaatcg
     6601 cttttagcgc gttgcataac tatgcacgaa agcgatgcgc gatcggtttc gtgcgttctg
     6661 aacaccgaac ggctcaaaac gacaagtttt ctccccctgg atggaaagtt ctgcacgagg
     6721 attttagatc ccatctccca catggaattt ttgtgacact tc
//
LOCUS       DAJTYZ010000182         6668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000182 DAJTYZ010000000
VERSION     DAJTYZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.182"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(37..413)
                     /gene="rnpB"
                     /locus_tag="OO596_003758"
     ncRNA           complement(37..413)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO596_003758"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(446..1591)
                     /gene="garK"
                     /locus_tag="OO596_003759"
     CDS             complement(446..1591)
                     /gene="garK"
                     /locus_tag="OO596_003759"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6719510.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1688..2578)
                     /gene="garR"
                     /locus_tag="OO596_003760"
     CDS             complement(1688..2578)
                     /gene="garR"
                     /locus_tag="OO596_003760"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6719511.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2608..3378)
                     /gene="garL"
                     /locus_tag="OO596_003761"
     CDS             complement(2608..3378)
                     /gene="garL"
                     /locus_tag="OO596_003761"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR6719512.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3394..4728)
                     /gene="garP"
                     /locus_tag="OO596_003762"
     CDS             complement(3394..4728)
                     /gene="garP"
                     /locus_tag="OO596_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6719513.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5103..>6668
                     /gene="garD"
                     /locus_tag="OO596_003763"
     CDS             5103..>6668
                     /gene="garD"
                     /locus_tag="OO596_003763"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6719514.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPV"
BASE COUNT         1629 a         1831 c         1657 g         1551 t
ORIGIN      
        1 agcaaaaacc cgccgaagcg ggtttttacg taaatcaggt gaaactgacc gataagccgg
       61 gttctgtcgt ggacagtcat tcatctaggc cagcaatcgc tcactggctc aagcagccta
      121 cccgggttca gtacgggccg taccttatga acccctattt ggccttgctc cgggtggagt
      181 ttaccgtgcc acggactgtt accagccgcg cggtgcgctc ttaccgcacc ctttcaccct
      241 tacctgatcc cgcttgcgcg ggccatcggc ggtttgctct ctgttgcact ggtcgtgggt
      301 ttccccccca ggcgttacct ggcaccctgc cctatggagc ccggactttc ctcccctccg
      361 cccgtctccc ccgaagagga cgacgacgaa gcggcgactg tctggtcagc ttcggcgcgc
      421 agtatagagg gtttgcgcgc ccttgtcacc ccgcgttgcg cattccaatc gccagtgtcg
      481 cggcgatatt acgtgaagca cggcagatat tgtcataagc cccgcggaat gcttcgtcca
      541 acgtacctat gctggtcaat acgctgaaga ccgcatcgat gccatgctga tgtacaacgc
      601 caacatcatc ggtcaggcta cccgcaatgc caatcaccgg tttatggtac ttcttcgcca
      661 cgtttgcaac gccaatcggt accttgccgt gaatactctg gctgtcaata cgcccttcac
      721 cggtgatcac cagcgtacaa tcgtgaatat gttcttccag attcagcgcc gtagtgacga
      781 tttcaatacc acttttcagt tccgcaccaa gaaacgccat cagcgccgcg cccataccgc
      841 ccgcagctcc tgcgccgggg acatctttca catcaacatg cagcgctttt ttaatgacat
      901 cggcatagtg agagaggtta ttgtccagct caacaatcat cgcttcactg gctccctttt
      961 gtgggccaaa gatgcgcgat gcgccgttat cgcccaccag cggattggtg acatcacaag
     1021 cgacgcgaat gacgcaatct tttaagcgcg gatcgaggcc ggaaatatca atatcattca
     1081 gagtattaag actaccgccg ccaaaaccaa tttcattgcc gttggcgtcg cataatttcg
     1141 cccccagcgc ctgtaccatg cctgcgccgc catcatttgt agcgctgccg ccaatgccga
     1201 taataatgtt tgttgcactg ctctccagcg cctgcaggat taactcgcct gtgccgcgtg
     1261 aagtggttac gagtggatcg cgtttttccg caggtaccag ctccagccca ctggccgccg
     1321 ccatttcaat aaacgcggtt ttgccatcgc cggagatccc ccaactggca ttcactttct
     1381 cgcccagcgg ccctgtaacc caggcgtgac gttcagcccc ctgggtggct gcaatcatcg
     1441 cttccaccgt tccttcacca ccgtcggcaa ccggaacaga aacatactgt gcatcaggaa
     1501 aaatttcccg aaatcctttt tctatcgcct gcgcaacctc gctggcagat aaactttctt
     1561 tataagagtc tggggcgatt acgattttca tacctatgcc tgttaccaca tgacgccgga
     1621 gggcgtttct cttattcggc ctagattcca ggcccggatt gcaatacacc atccgggcac
     1681 gacgtcatta acgagtaact tcgactttcg ccagtttttc gtagtagcac gccagggcgc
     1741 tatgatccgc cgttcctaaa ccatctgcac gcagtgcctg catcatctcc ataaccgcag
     1801 ctgtgagcag cagttgtgcg ccgacgccgt gagaagtatc cagcgcattc gccagatcct
     1861 taatatgcag atcaatgcgg aagcccggct tgaagttgcg gtccatcacc atcggcgctt
     1921 tggcatccag cacggtactg cccgccagcc caccgcgaat tgcctgataa accaggtccg
     1981 ggttaacgcc cgctttggtt gccagcgtta acgcttctga catcgcagca atattcagcg
     2041 ccacaatgac ctgatttgcc agtttggtga cgttacctgc accgatttcc ccggtatgca
     2101 ccacggaacc cgccatcgct ttcatcaaat catagtattt gtcgaaaata gccttgtcgc
     2161 cgcccaccat cactgacagc gtaccgtcga tggctttcgg ttcaccgccg ctcaccggag
     2221 catccagcat atcaatgcct ttcgctttca gcgcttcgct gatttcacgg cttgccagcg
     2281 gtgcgataga actcatatcg atcaataccg tacctggctt cgcgccttca ataatgccat
     2341 tctcacccag cgccacctct ttcacatgag gggagtttgg cagcatggtt atgatgacgt
     2401 cgcactgttc agcgatcgct ttagccgtag acgctgtttc tgcacctgca gcaatcacgt
     2461 cagcaatagc ttctgggtta cggtcagcaa ccaccagcga gtaacctgct ttcagaaggt
     2521 ttttactcat tggtttaccc ataatcccca ggccaataaa accaactttc atagtcatat
     2581 caaccatctc tcttgttgcg gtggtggtta ttttttaaag gtatcagcca gtttctgagt
     2641 ggcagagcgg aagacgccaa gatcgctgcc gacagccaca aacgtcgcgc cccattccag
     2701 ataacgacgc gcatcggctt cgaccggcgc gagaataccg ctgggtttgc cgtgcgcgct
     2761 ggcacggtta aaaatatgct gaattgcttt ttgtacatcc ggatgcgagg cattgccgag
     2821 atggcccaat gccgcagcca gatcgctggg gccgacgaag ataccgtcta caccttcggt
     2881 agcggcaata gcatcgacgt tatctacgcc ctgctgactt tctatctgga ccagaatagt
     2941 aatgttcttg ttcgactgag cgaaataatc cgccacggtg ccaaacatat tggtgcggtg
     3001 agaaacggag acgccgcgaa tgccttccgg cgggtaacga gttgatgcca ccgcctgctc
     3061 tgcttcctct tttgtttcta caaaaggaat caggaagtta tagaaaccga tatccagaag
     3121 acgcttaata attaccggct cgttggtcgg cactcgcact actggcgcgc tggcgctgcc
     3181 tttcagggcc attaactgcg gaataaacgt ggaaatatcg tttggcgcat gttcgccatc
     3241 cagcaccagc cagtcaaacc cagccaaacc aagaacttca gtgctaatcg ggttagagag
     3301 tgctgaccag caaccaattt gtacctgttt cgcagccagt gcggctttga atttattcgg
     3361 gaaaacatcg ttattcatcg cttatacctt tgcttatttc tgcaattcca tacgtttaat
     3421 gtcgccaact acgaagaggt agcagaccat cgccatcagc gctgaacatc ccacgaaaac
     3481 cagtgctgca ttgaaggagt gcagttcact taccaggtag ccaatcacca gtggagtgac
     3541 aatggaggca acattgccaa agacgttaaa gacgccgcca cagaggccaa caatctcttt
     3601 cggcgcggtg tcagaaatca ccggccagcc cagagcacca aatcctttgc caaaaaaagc
     3661 cagcgccatc agcatgacca ccagcgtggt gttgttggtg tagttacata agatgatggt
     3721 ggaaaccagc aacattccca gcacaatcgg tagcttacgt gccagggtca gggataaacc
     3781 gcgtttgatc agataatccg agaagacacc tcccagcacg ccgcccgcaa aaccacacag
     3841 tgccggaatc gaggcgacca gacccacttt cagaatcgac atgccttttt cctgcaccag
     3901 ataaatcggg aaccaggtga ggaagaacca ggtgatggtg ttgataaaat attgtccgaa
     3961 aaatacgccc aacatcatgc ggttagagag caattgcttg atgtaatgca gtttgggacc
     4021 gcttgctgcc gcgctgcccg gctttttgtg gtccatatcg accaccgcgc cattttcaga
     4081 gataaacttc agctcttccg cagacatacg tgggtgatct gtcgggttat gaatcaactt
     4141 gatccacagc gccgtcagca caaaaccaat cccccccata acggtgaaga cgtgctccca
     4201 gccccaagcg aaagtcagcc agccaagcag cggcgaaaag agcgccagcg agaaatattg
     4261 cgccgagtta aagatggcgg aggcagtacc acgttctttc gtcgggaacc aggcggcgac
     4321 aatacgggcg ttcgccggga atgatggcgc ttccgagaac cccagcataa agcgcataaa
     4381 gaacatggag atccctgccc aggccagcgg gaacatatca acaaagcctt gcaggaaggt
     4441 gaacagcgac cagaaaaaga ggctgtaggt gtaaactttt ttcgagccaa acttatcaag
     4501 caaccagccg ccggggattt gcatcagcaa gtaggcccag ccaaaagcag agaagatgta
     4561 acccatcgaa accgcactta actgcaactc ttttgccact tcggtaccag caatagacag
     4621 cgttgcacga tcggcgtagt taacggcggt aacaataaaa ataatcagta atattaaata
     4681 gctggtatgc acgcctttct tttttacgtc aacggtgtcc agaatcattt tatttacctc
     4741 gggtacttat gctgattttt attattatgg ggaaggtgtt atttatgagt tttatttatg
     4801 ccgttacgac aatgaactcg ggaattagta taagcagcgg gagaataata atcattgtgc
     4861 aaatgctaat ttaatcagta ctatttaaat attattttga gcatatgcac ataaggttgc
     4921 gcgctaaagc acagatttgc gctttacctt accgggcggc actgcaatcc ctgaaatgat
     4981 tgacattgat cacatttctg cgtttaaact cctgacattc ttatttcacc caatgaagtc
     5041 atttattttt aaatgagacc agatcctcat tttaataacc cctggctgga gaatattgca
     5101 caatggccaa catcgaaatc agacaagaaa cgccaactgc gttttatata aaagttcacg
     5161 acacagataa tgtggcaatt attgttaatg ataatggcct gaaagcagga acgcgttttc
     5221 cggatgggct ggaattaatt gaacatactc cccaggggca taaagtcgca ttgctggaca
     5281 ttccagctaa tggtgaaatt attcgttatg gcgaagtgat tggttacgcc gtgcgcgcaa
     5341 tcccacgcgg aagctggatc gacgaatcaa tggttgtact accggaagcg ccgccgttac
     5401 acacgctgcc actggcaacc aaagtcccgg cccccttacc gccgctggaa ggatacacct
     5461 ttgagggcta tcgcaatgcc gatggcagcg tgggcaccaa aaacctgctc ggcatcacca
     5521 ccagcgtcca ctgtgtggca ggcgtggtgg actatgtagt aaaaatcatt gaacgcgatc
     5581 tgctaccgaa atacccgaac gtcgatggcg tggtggggct gaatcatttg tacggctgtg
     5641 gcgtggcgat taacgcaccg gcggcagttg tgcctattcg taccatccac aatatttcgc
     5701 tgaaccctaa ctttggcggc gaagtaatgg tgattggtct gggttgtgaa aagttgcagc
     5761 ctgagcgcct gctggtcggc acggatgatg tgcaagctat tccagtagaa agcgccagca
     5821 ttgtcagttt gcaggatgaa aagcatgtcg gctttcagtc catggtcgag gatattttgc
     5881 aggtcgccga acgccatcta caaaaactga atcaacggca gcgagaaacc tgcccggctt
     5941 cagaactggt cgttggtgtg cagtgcggtg gcagcgatgc gttttctggt gtaacggcaa
     6001 acccggcggt tggctatgcg tctgatctac tggtgcgctg cggcgcaacg gtgatgtttt
     6061 cagaagtaac ggaagtgcgt gacgcgatcc atctgctgac accacgcgca gtgaacgaag
     6121 aggtcggcaa acggctgctg gaggagatgg agtggtacga taactatctc aatatgggaa
     6181 aaaccgaccg cagcgccaac ccttcgccgg gcaacaagaa aggcggtctg gcaaatgtgg
     6241 tggagaaggc actcggctcc attgctaaat cgggtaaaag tgcaattgtt gaagtgctgt
     6301 cgcccggtca acgcccgact aaacgcggat taatttacgc cgcgacgcca gccagcgatt
     6361 ttgtctgtgg cacgcaacag gtggcttcgg gtatcaccgt acaagtgttt acgaccggcc
     6421 gtggtacgcc gtacggcctg atggcggtac ccgtaattaa aatggcaacc cgcaccgagc
     6481 tggcgaaccg ctggtttgat ttaatggata ttaacgcggg cactatcgct accggcgaag
     6541 agagcattga agaggtgggc tggaagttgt tccactttat tctcgacgtc gccagcggga
     6601 agaagaaaac cttctcggat caatgggggc tgcataacca gctggcggtg tttaacccgg
     6661 caccggtg
//
LOCUS       DAJTYZ010000183         6651 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000183 DAJTYZ010000000
VERSION     DAJTYZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.183"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1252
                     /locus_tag="OO596_003764"
     CDS             <1..1252
                     /locus_tag="OO596_003764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719515.1"
                     /translation="ENMLLEYVERKISLALSKYPKVRDVIKFFYLYIASLFGIILNKN
                     KTVIQSKIYEISIDDSEESFFGYYDHSPMSSNGRYVLFHSSAFSTKRHPKKVKYISIC
                     VKDLLNNKVYKLYDTRAFNWQQGSRLMWIDDDNIIFNDYENNGYISVVYSLSLMKVIK
                     KINYPIYDVNNYKAVTLDFSWLAKYDSDYGYYNKKSFSTDISIINLNTGGIELFLSLD
                     EMLKRTNFKCNIDVEHVVNHFMFAPDGRSVMFIHRYYTPKGKRERLIHWNLINDNVRV
                     LINESIISHCCWNGNDEIIGFFGAEIDSLNYYRLSIESCNTEKLFFDARKYSDGHPTI
                     VHNRYIISDTYPDKNRIKKLFVYDLVKNDYRELGLFYESMSFFSYSRCDLHPRISVDN
                     RFLFVDSVHSGKRKLYFMRSGICE"
     gene            1245..2231
                     /locus_tag="OO596_003765"
     CDS             1245..2231
                     /locus_tag="OO596_003765"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6719516.1"
                     /translation="MSDVLVSLIIVCFNAEKYIEKSLLAFINQDVGLDKFELIIVDGD
                     SSDNTISIVQDVFSKHSNIKHKIINNKKRTLATGWNIGVLEANGKFVCRVDAHSDIPN
                     NYISKLLDDYFNIMQFDDSVVGVGGVLTNSYKTKFGSIVADFYASKFGVGNSPFRCVD
                     KNNRLKKTDTAVFALYNKDVFFDVGLFNEVLDRNQDIDFHKRVLSNNLSLYTDNSLFV
                     EYYVRDNFKDFIKKGFLDGFWVVMSGAYYFRHIVPLFFVLYLIVSFSLFFATGDYIYL
                     SFLFFYFLISILFSIRDGRSFIGRVFLPFIFLSYHISYGCGSLLSFLKRYFK"
     gene            2228..3382
                     /locus_tag="OO596_003766"
     CDS             2228..3382
                     /locus_tag="OO596_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HCR6719517.1"
                     /translation="MKNFIPFALPEIGEEEIAEVIDSLRSGWITTGPKAKQFEQEFSN
                     YLGANVQSLAVNSATSGLHLALEAVGVKPGDQVIVPSYTFTATAEIVRYLGADPVIVD
                     VDRKTFNISVDAIEKAITNETKAIIPVHFAGLACDMDSILSIAKKYDLKVVEDAAHAF
                     PTTYKGSKIGTLDSDATVFSFYANKTMTTGEGGMVVSKNKDIIERCKVMRLHGISRDA
                     FDRYQSKTPSWFYEVVAPGFKYNMPDICAAIGIHQLRKIDDFQKKRQRMAKIYDDALK
                     ELPLELPEWPTNASDIHAWHLYPIRLKTDSAINRDDFIKKLSDLGIGCSVHFIPLHKQ
                     PVWRDTYNLNASDFPVSEECYLNEISIPLYTKMTDQDQLFVIKSIRQLFM"
     gene            3399..3989
                     /locus_tag="OO596_003767"
     CDS             3399..3989
                     /locus_tag="OO596_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR6719518.1"
                     /translation="MKRIFDVIVAGLGLLFLFPVFIIVSMLIVADSKGGVFFRQYRVG
                     RFGKDFRIHKFRTMFIDSEKKGRITVGQDARVTRVGWYLRKYKIDELPQLIDVLSGTM
                     SLVGPRPEVREFIDEYPDDIREKVLSVRPGITDLASIEMVDENEILSSYDDPRRAYID
                     IILPIKQRYYLDYVANNSVKYDCVIIWKTIIKILSR"
     gene            4145..5923
                     /locus_tag="OO596_003768"
     CDS             4145..5923
                     /locus_tag="OO596_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR6719519.1"
                     /translation="MWFAAAILTIFTVFIFIRIGLYRAVLRYVSAKIMLLIPVGILAS
                     TLSLVVISYSLSIMLPRTVVGIYFLVLLLLTSGSRLLFRMILNYGVKGSAPVLIYGAG
                     ESGRQLLPALMQAKEYFPVAFVDDNPRLHKAVIHGVTVYPSDKLSYLVDRYGIKKILL
                     AMPSVSKSQRQKVITRLEHLPCEVLSIPGMVDLVEGRAQISNLKKVSIDDLLGRDPVA
                     PDAKLMAENITGKAVMVTGAGGSIGSELCRQIVRYKPAKLVLFELSEYALYAIEKELS
                     ALCDKEVLNVPVIPLLGSVQRQNRLQMVMKSFGIQTVYHAAAYKHVPLVEHNVVEGVR
                     NNVFGTLYCAESAIESGVETFVLISTDKAVRPTNTMGTTKRLAELVLQALSARQSQTR
                     FCMVRFGNVLGSSGSVVPLFEKQIAQGGPVTLTHRDIIRYFMTIPEASQLVIQAGAMG
                     HGGDVFVLDMGDPVKIYDLAKRMIRLSGLSVRDDKNPDGDIAIEVTGLRPGEKLYEEL
                     LIGDSVQGTSHPRIMTANEVMLPWQDLSLLLKELDQACHDFDHERIRSLLLQAPAAFN
                     PTDDICDLVWQQKKSLLSQASNVIRL"
     gene            6042..6549
                     /gene="aqpZ"
                     /locus_tag="OO596_003769"
                     /pseudo
     CDS             6042..6549
                     /gene="aqpZ"
                     /locus_tag="OO596_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
BASE COUNT         1868 a         1048 c         1459 g         2276 t
ORIGIN      
        1 tgagaatatg ttgttagaat atgttgaaag aaaaatttcc ttagccttga gtaagtatcc
       61 taaggtaagg gatgttatta agttctttta tttatatatc gcatcattat tcggaattat
      121 tttgaataaa aataagacgg ttattcaatc aaaaatatac gagatttcaa ttgatgattc
      181 tgaagaatca ttttttggct attatgacca tagtccaatg agctctaatg ggcggtacgt
      241 attgttccac tctagtgcgt ttagcactaa acgacatcca aagaaagtta agtatatatc
      301 tatttgcgta aaagaccttc ttaataacaa agtttataag ctatatgata cgcgagcatt
      361 taattggcag cagggaagcc gattaatgtg gattgatgat gacaatataa tttttaatga
      421 ctatgaaaat aatggataca ttagtgttgt ctattctttg tctttgatga aggttataaa
      481 aaaaataaac tatccgattt atgatgtgaa taattacaag gctgtgacgt tagatttctc
      541 atggctggct aaatatgata gcgattatgg ttattataat aaaaaatcat tttctacaga
      601 tatttcaatc attaatttga atacgggggg aatagaatta tttttatcct tagacgaaat
      661 gctaaagaga actaatttta aatgtaatat tgatgttgaa catgtggtca atcattttat
      721 gtttgctccc gatggacgtt ccgttatgtt catacatcga tactatacac ctaaaggaaa
      781 gcgtgaaagg ttaatacatt ggaatttaat aaatgataat gttcgagtcc taataaatga
      841 atcgattatt agtcattgtt gttggaatgg gaatgatgaa attataggtt tttttggtgc
      901 agaaatagat tcgctaaatt attatagatt gtcaattgaa tcctgtaata cagagaaatt
      961 gttttttgat gcaagaaaat attctgatgg acatcctact atagttcata atagatatat
     1021 tatatctgat acttacccag ataaaaatag aattaaaaag ttgtttgttt atgaccttgt
     1081 caaaaatgat tatcgcgagc ttggattatt ttatgagtca atgagttttt tttcttattc
     1141 tcgatgtgac ttacatccaa ggatctcggt tgataataga tttttgtttg ttgattcagt
     1201 tcactcaggg aaaagaaaac tatattttat gaggagtggt atttgtgagt gatgttctag
     1261 tatctttaat tatagtttgc tttaatgcag agaagtatat tgaaaaatct cttttggcat
     1321 ttattaatca agatgttgga ttagataaat ttgaattgat tattgtagat ggggattcat
     1381 ctgataatac aatatctatt gttcaggatg ttttttctaa acatagcaac attaagcata
     1441 aaattatcaa taataaaaaa agaactcttg ctacgggttg gaatattggg gtgctagaag
     1501 ctaatggtaa gtttgtgtgt agagttgatg cacatagtga tattccaaat aactatatat
     1561 ctaaattatt agatgattat tttaatatta tgcagtttga tgatagcgtt gttggtgttg
     1621 gaggtgtatt aactaattct tataaaacta agtttggttc aattgtagcg gatttttatg
     1681 catcgaaatt tggtgttggt aattctccat ttaggtgcgt agacaaaaat aatcgactaa
     1741 aaaaaacaga tacagctgtc tttgctttat ataataaaga tgtgtttttt gatgttggac
     1801 tttttaatga agtattagat agaaatcaag atattgattt tcataagaga gttttaagca
     1861 ataatttgtc attatataca gataatagtt tatttgttga gtattatgtt agagataatt
     1921 ttaaagattt cataaagaaa ggttttcttg atggtttttg ggttgttatg tctggagcat
     1981 attattttag acatatagtg ccactttttt ttgttttgta tttaattgta tctttttctc
     2041 ttttctttgc tactggtgat tatatatatt tatctttttt atttttttat tttcttattt
     2101 ctattttgtt ttcaattcga gatgggcgaa gttttatagg tagagtattt cttcctttta
     2161 tatttttgtc ttatcatatt tcttatggat gtggatcgtt attatctttt ttgaaaaggt
     2221 attttaaatg aaaaatttta ttccttttgc gttacctgaa attggcgaag aagaaattgc
     2281 agaggtaatt gactctttac gttcaggttg gattacgaca ggtcctaagg ctaagcaatt
     2341 tgaacaagaa ttttctaatt acctaggagc gaacgttcaa tcattagctg ttaactctgc
     2401 tacgtcgggc ttacatttgg ctcttgaagc tgttggcgta aagccgggag accaagttat
     2461 tgtcccatca tatacattca ctgctactgc cgaaattgtc aggtaccttg gtgctgatcc
     2521 tgtaattgtt gatgtagatc gtaaaacatt taatatatca gttgatgcca ttgagaaggc
     2581 tattactaat gaaacaaagg cgattattcc agtacacttc gctggattag cttgtgacat
     2641 ggattcaatc ttatcaattg ctaaaaaata tgacctaaag gttgtcgagg atgccgctca
     2701 tgcatttcct acaacatata aaggaagtaa gataggaacg cttgattcag atgctacggt
     2761 ttttagcttc tacgccaata aaactatgac aaccggtgaa ggcggaatgg ttgtttcaaa
     2821 aaataaagat ataattgagc gttgtaaggt aatgcgttta catggaatca gtcgtgacgc
     2881 ttttgaccgg taccagtcta aaactccttc ttggttttat gaggttgtag ctccagggtt
     2941 taaatacaat atgcctgata tctgtgcggc aatcggtatt catcaactta gaaagatcga
     3001 tgattttcag aaaaaacgtc aacgaatggc aaaaatttac gatgatgcgt taaaagaatt
     3061 gccacttgaa ttgcctgaat ggcctactaa tgctagtgat attcatgctt ggcatctata
     3121 tcctatccgc ttaaaaactg attcggctat taatcgcgat gattttatta agaagttatc
     3181 agatcttgga attggttgtt ctgtccattt tataccgttg cataagcaac cggtttggcg
     3241 tgatacatat aatttgaacg ccagtgactt tccagtttct gaggagtgtt atttaaatga
     3301 aatatctatt cctctttata ctaaaatgac ggatcaagat cagttgttcg ttatcaaatc
     3361 gattagacaa ttatttatgt aatggtattt tatattaaat gaaacgtatt tttgatgtta
     3421 tcgtggcagg cttaggcctg ctttttctat ttcctgtttt tatcattgtg tcaatgttaa
     3481 ttgttgctga ttctaaaggg ggggtttttt ttaggcagta tagagttggg agatttggga
     3541 aagattttag gatacataaa tttagaacga tgtttatcga ttcagaaaaa aaaggacgga
     3601 taacagttgg tcaagatgct cgggtaacca gagttggatg gtatttacgg aagtacaaaa
     3661 tcgatgagct tcctcaattg atagatgttc tttctggaac aatgagtttg gttggcccaa
     3721 gaccggaagt gagggagttt attgatgagt atcctgatga tataagggaa aaagttttat
     3781 cggttaggcc agggataact gacttagcat ctatagaaat ggtagatgaa aatgagattt
     3841 tgtctagtta tgatgaccca cgtagggctt atatagatat aattcttcca atcaagcaaa
     3901 gatattattt agattatgtt gctaacaatt cagtaaagta tgattgtgtg ataatttgga
     3961 aaactattat taagattttg tcgcgataat aaggtagtgt aggatgattg atagaatatt
     4021 ggagctgcca agaattgtta agagaggtat catcatctgc attgatgtag ttatggtgat
     4081 attctcattt tggttgtctt attggttgag gcttgatgag caaacggctt ttcttagtgc
     4141 accgatgtgg tttgctgcag ctattcttac catatttacc gtgtttatat ttatcaggat
     4201 tgggctttat cgggcagtct tacggtatgt tagtgcaaag ataatgttgc taataccagt
     4261 tggtattctg gcctcaacgt tatctcttgt cgttatatca tattcgctat ccataatgtt
     4321 gccgcgcact gttgtcggaa tttatttttt ggttttactt ttactgacat caggctctag
     4381 attgcttttt agaatgatac ttaactatgg agttaagggt agtgcgcctg ttttgattta
     4441 tggcgctggt gaatctggcc gacaattatt gccagcatta atgcaggcaa aagaatattt
     4501 tcctgtggca tttgtggatg ataatcctcg cttgcataag gctgtcattc atggtgtaac
     4561 agtttatccc tcggataaac tgagttacct tgtagatcgc tatggtataa agaaaattct
     4621 tttggcgatg ccgagcgtca gtaagtcaca aaggcagaaa gtgattactc gtttagagca
     4681 tctaccgtgt gaagttctct ctattccggg tatggtcgat ttagtcgaag gtcgagcaca
     4741 aatcagtaat ctaaaaaaag tatcgattga tgacttacta ggtcgtgatc cggttgctcc
     4801 tgatgccaaa ttgatggccg aaaacattac tggcaaagcc gttatggtca ctggggcggg
     4861 aggctcgatc ggctctgagc tttgtcgtca aattgttcga tataagccgg ccaaattggt
     4921 tctatttgaa ctgtctgaat atgccctcta cgctattgag aaagagctct cggcgctgtg
     4981 tgacaaagaa gttttgaatg ttccagtgat ccctctgttg ggctcggtgc agcgtcagaa
     5041 tcgcttacag atggtgatga agtcctttgg tattcaaacg gtttatcatg cggccgctta
     5101 taaacatgtg cctctggttg agcataatgt ggtggaaggg gtacgtaata acgtgtttgg
     5161 taccttgtac tgcgctgagt cagcgatcga aagtggcgtt gaaacttttg tgttgatttc
     5221 caccgataaa gcggtgcgcc cgaccaacac tatggggaca actaagcgtc tggccgaatt
     5281 ggtattgcag gctttgtctg cacggcaaag ccaaactcgc ttttgtatgg tgcgatttgg
     5341 taatgtactc ggttcttcgg gctctgtcgt gccgttgttt gaaaaacaga ttgcccaagg
     5401 tgggccagtt accttgactc atcgtgacat tattcgctat ttcatgacaa ttccggaagc
     5461 atcacagttg gtgattcaag cgggggcgat ggggcatggc ggcgatgtct ttgtcttaga
     5521 catgggcgat ccggtcaaga tttatgactt agccaaacgc atgatccggt taagtggctt
     5581 gagtgtacgg gatgataaaa atccagatgg cgatattgcc attgaagtta cgggattacg
     5641 tccaggtgag aaactgtatg aagaattact gattggtgat tcagttcaag gtacctctca
     5701 tccacgaatt atgacggcca acgaagtgat gctaccgtgg caggatctat cgctcttact
     5761 taaagagctg gatcaagctt gtcatgactt tgatcatgag cgaattcgca gtttgttgtt
     5821 acaagcacca gcggcattca atccaactga tgatatttgc gatctagttt ggcagcagaa
     5881 aaaatcgctg ttatcacaag cgagcaatgt cattcgcctg tgattgctta ggtttaacct
     5941 tccacaccaa ttcttcacct ctcttacaaa tccccgctag gcggtacatc gtgaccgcct
     6001 ttaccctgat gcctgctctt taacaaacag gacatcagtg tatgtttaaa ccttttagcg
     6061 ccgaattttt cggcactttc tggctggttc tgggtggctg tggtagcgcc ttgatctctg
     6121 ctgctttccc acagttaggt ataggctttt tgggcgtggc gttggcgttt ggtctgacag
     6181 tagtcaccat ggcttatgcg gtcgggcaca tctctggtgc gcattttaac cccgcggtga
     6241 ccttgggtct gtgggccggt ggacgcttcc cagcagcgcg cgtgttacct tacattatcg
     6301 ctcaggttat cggcggtatt gccgctgcgg cagtgctgta tggtatcgcc agcggtaagg
     6361 ctgggtttga tgcgacaacc agcggttttg cggctaatgg ttatggcctc cattcacctg
     6421 gcggctatgc gttaagcgcc tgtatgctga gcgagtttgt cctcagtgcg ttttttgtcc
     6481 ggagcgacag aaaaacgcgc tcctgcgggc tttgcgccac tggcgattgg tctggtaatc
     6541 accccgtaaa ttaaccagcg tcaaaagtag aattttctcg taccataaac gcaggagatt
     6601 ctttatgaaa acatcaaaat ttaccgacag ccaaatcatg gcgatcctca a
//
LOCUS       DAJTYZ010000184         6647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000184 DAJTYZ010000000
VERSION     DAJTYZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.184"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..737)
                     /locus_tag="OO596_003770"
     CDS             complement(<1..737)
                     /locus_tag="OO596_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR6719520.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="OO596_003771"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="OO596_003771"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6719521.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="OO596_003772"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="OO596_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6719522.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="OO596_003773"
     CDS             4397..4678
                     /locus_tag="OO596_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6719523.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4921..5463
                     /locus_tag="OO596_003774"
     CDS             4921..5463
                     /locus_tag="OO596_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6719524.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5543..6217
                     /locus_tag="OO596_003775"
     CDS             5543..6217
                     /locus_tag="OO596_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6719525.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6233..>6647
                     /locus_tag="OO596_003776"
     CDS             6233..>6647
                     /locus_tag="OO596_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6719526.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1756 a         1507 c         1593 g         1791 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agagggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg atttttattg atatcaacaa taccatttac ataaatctac attaattgat
     4741 agatgcgata aatagttaat actcttaaat atttgttgta tttatattgc aatggttaat
     4801 ttgtttcaaa taaataaaac aatgtgttta cattgcggta acttttttga tgtgagaaat
     4861 ttgtttccaa gagatacaga ctcttaacaa ccaaaaaaaa attttattaa ggaacatatt
     4921 atgaaacgtt caattattgc tgccgctgtc ttttcttctt tttttatgag cgctggagta
     4981 tttgctgcag acgttgatac cggaacatta actattaagg ggaatattgc agaatctccg
     5041 tgtaaattcg aagcgggtgg tgattcagta agtattaata tgccgactgt accaaccact
     5101 gtctttgaag gtaaagctaa atattctacc tatgatgatg cagtcggtgt aaccagcagc
     5161 atgttaaaaa ttagctgccc gaaagaagtt gctggtgtaa aactctcgtt gattaccaac
     5221 gataaaataa ccggtaacga taaggcgata gccagtagca acgataccgt gggttactat
     5281 ctctatttag gtgataacag cgatgtcctg gatgtttctg caccttttaa cattgagagt
     5341 tataaaacag cggaaggtca atatgctatt ccgtttaaag caaaatacct gaaactgaca
     5401 gataactcag tgcaatcagg tgatgtgtta tcttctctgg ttatgcgtgt ggcgcaggat
     5461 taataaataa aattgtaagt ttctccaggt aataaacatg gccgctatcc catggcggcc
     5521 ttttaattta agaggcatta aaatgaaagg attattatct ttactcattt tttctatggt
     5581 ccttcctgca catgccggaa ttgttatcta cgggacgcgc attatttacc cggcagaaaa
     5641 taaagaagtg atggtgcagt tgatgaacca gggaaaccgt tcttcgctgc tgcaggcgtg
     5701 gattgatgat ggcgatacgt cattaccacc agaaaaaatt caggttcctt tcatgttaac
     5761 gccaccagtg gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc
     5821 gaataaactg cccactaata aagaaagcat tttttatctg aatgttctgg atattccacc
     5881 aaatagtcca gagcaagaag gtaagaatgc actgaagttt gcgatgcaaa acagaattaa
     5941 gttgttttac cgaccagcgg gtattgctcc ggtaaataaa gcgacattta aaaaattgct
     6001 ggtaaatcgc agtggcaatg gtttggtgat aaaaaatgac tcagctaatt gggtgacgat
     6061 ttcggatgtc aaagctaata atgtcaaagt caattatgaa actattatga ttgccccctt
     6121 agaaagtcag agtgttaatg tcaaaagtaa taatgcaaat aactggcatc tgaccattat
     6181 cgatgaccat ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag
     6241 aatgacccca cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc
     6301 tgaagaaacc tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa
     6361 tattcgtctt gatgataatc aacccttacc ggggcagtat gacatcgata tttatgtcaa
     6421 taagcaatgg cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt
     6481 atcaagagaa gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca
     6541 atgtttaaca tttgagcaac ttgttcaggg tgggagctat acctgggata tcggggtttt
     6601 tcgtctcgat ttcagtgtcc cgcaggcctg ggtggaagaa ctggaaa
//
LOCUS       DAJTYZ010000185         6614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000185 DAJTYZ010000000
VERSION     DAJTYZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.185"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            68..397
                     /locus_tag="OO596_003777"
     CDS             68..397
                     /locus_tag="OO596_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6719527.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            407..1105
                     /locus_tag="OO596_003778"
     CDS             407..1105
                     /locus_tag="OO596_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6719528.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1110..1853
                     /locus_tag="OO596_003779"
     CDS             1110..1853
                     /locus_tag="OO596_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6719529.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            1790..2392
                     /locus_tag="OO596_003780"
     CDS             1790..2392
                     /locus_tag="OO596_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6719530.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2453..5932
                     /locus_tag="OO596_003781"
     CDS             2453..5932
                     /locus_tag="OO596_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6719531.1"
                     /translation="MGKGSRKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6000..6599
                     /locus_tag="OO596_003782"
     CDS             6000..6599
                     /locus_tag="OO596_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6719532.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT         1505 a         1599 c         2103 g         1407 t
ORIGIN      
        1 aagcggccgc ggactttatg caacagcagg cccgtgatgg tggccggtta agtggagcat
       61 accggtaatg gagacgtttc actggaaagt gcgcccggat atgaatgtgg tatcagagcc
      121 gaaagtggtg acagtgaagc tgggcgatgg ttatgaacag cgtcgtgcgg cgggactgaa
      181 taaccagttg tcgacttaca gcgtgaagat acgtgttcgt aaaggtgaac acccattttt
      241 aaaagccttt ctggaacggc acggtggcgt ccgcgcattt cagtggacgc caccttatga
      301 ctggaagccg atcagggtgg tttgtcgtaa atggtcggca agcgtggggg cgttgtgggt
      361 aaccataacg gcagattttg aacaggtcgt ggcataggag gctctgatgc aggatattcc
      421 acaggaaaca catcatgaga cgacacgcct cactcagtca gcccaggtgg tgctctggga
      481 aatcgatctg acagaggtcg gtggtgaacg ttattttttc tgtaatgagc agaacgaaaa
      541 aggtgagccg gttacctggc aggggcggca gtatcaggca taccccattc aggggacggg
      601 atttgaactg aacggcaagg gcagtgctgc ccgtccgaca ctgacggttt ctaacctgca
      661 cggaatggtc accgggatgg cggaagacct gcagagtctg gtcggcggaa cggtggtcag
      721 gcgtaaggtt tacgcccgtt ttctggatgc ggtgaacttc gtcaacggaa acagcgacgc
      781 cgatccggaa caggaggtga tcagccgctg gcgcatcgag cagtgcagcg aactgagcgc
      841 ggtcagtgcc tcttttgtgc tggccacacc aacggagacg gatggcgcgg ttttcccggg
      901 gcgtatcatg ctggcgaata cctgtatgtg gacttaccgt tctgatgagt gtggttacac
      961 gggcagggca gtggctgacg agttcgacaa accaacgacg gatatccgga aggacaaatg
     1021 cagcaagtgt atgcgcgggt gtgagttgcg caacaatacc ggtaatttcg gcggtttcct
     1081 ttccatcaat aaactttctc agtaaatcca tgacacagac agaatcagcg attctggcgc
     1141 acgcccggcg atgtgcgcca gcggagtcgt gtggcttcgt ggtaagaacg ccggagggag
     1201 acaggtatct tcccagcgag aatatctccg gtgagccgga ggaacggttc cggatggctc
     1261 cggaggactg gctgcgggca caaatgcagg gtgagattgt ggcgctggtc cacagtcatc
     1321 ccggtggtct gccctggctg agtgaggctg accggcggct gcaggtgcag agtgatttgc
     1381 cgtggtggct ggtctgccgg ggggcgattc acaagttccg ctgtgtgccg catctcaccg
     1441 ggcggcgctt tgagcacggg gtgacggact gttacacgct gttccgggat gcttatcatc
     1501 tggcagggat tgagatgccg gattttcatc gcggggatga ctggtggcgt aacggtcaga
     1561 atctctatct tgacaatatg gaggtgacgg gtttttaccg tgtcgcactg acagaggcac
     1621 agccgggcga tgtgctgctg tgctgttttg gttcatcggt gccgaatcat gccgccattt
     1681 actgcggtga cggcgggctg ctgcaccata ttcctgaaca actgagcaaa cgagagaggt
     1741 acaccgacaa atggcagcga cgcacacact ccctctggcg tcaccgggca tggcgcgcat
     1801 ctgcctttac ggggatttac aacgatttgg tcgccgcatc gaccttcgtg tgaaaacggg
     1861 ggctgaagcc atccgggcac tggccacaca gctcccggcg tttcgtcaga aactgagtga
     1921 cggctggtat caggtgcgca ttgccgggcg tgatgcaggt gaaaccgaat tgtctgcccg
     1981 tcttaatgag ccgctggcaa atggtgcagt gatccacata gtaccgcgtc tggtgggagc
     2041 taaaagtggc ggtgtgtttc aggcggtgct gggggcggct gttatggcgg ttgctatatg
     2101 gatgccgggg gtaggaatta tggcgagtaa tctgctgttt tctctcggtg ccagtatgac
     2161 gcttggcggt gttgcacaga tgctggcacc gaaagccaga actccccgta cacagacaac
     2221 ggataacggt aagcagaaca cgtatttctc gtcactggat aacatggttg cccagggcaa
     2281 tgttctgccc gttctgtacg gtgaaatgcg cgtggggtca cgtgtggttt ctcaggagat
     2341 cagcacggca gacgaagggg atggtggtca ggttgtggtg attggtcgct gatgcaaaat
     2401 gttttatgtg aaaccgcctg cgggcggttt tgtcgtttat ggagcgtgag gaatgggtaa
     2461 aggcagcagg aaggggcata ccccgcgcga agcgaaggac aacctgaaat ccacgcagtt
     2521 gctgagtgtg attgatgcca tcagcgaagg gccggttgac ggtccggtgg atggattaaa
     2581 aagcgtgctg ctgaacggta cgccggtcct ggacagcgag gggaagacaa acttttccgg
     2641 tgttacggtg gtgttccgtg caggtgagca ggagcagaca ccgccggagg gttttgaatc
     2701 ctccggctcc gagacggtgc tgggtacgga agtgaaatat gacacgccga tcacccggac
     2761 catcacgtcg gcaaacatcg accgtctgcg cctgaccttc ggtgtgcagg cactggtgga
     2821 aaccacctca aagggtgacc ggaatccgtc ggaagtccgc ctgctggttc agatacagcg
     2881 taacggtggc tgggtgacgg aaaaagacat caccattaag ggcaaaacca cttcgcagta
     2941 tctggcctcg gtggtggtgg gtaacctgcc gccgcgcccg tttaatatcc ggatgcgcag
     3001 gatgacaccg gacagcacca cagaccagct gcagaacaaa acgctctggt cgtcgtacac
     3061 cgaaatcatc gatgtgaaac agtgctaccc gaacacggcg ctggtcggcg tgcaggtgga
     3121 ttcagagcag ttcggtaacc agcaggtgag tcgcaattat catcttcgcg ggcgcattct
     3181 gcaggtgccg tcgaactata acccgcagac gcggcaatac agcggtatct gggacggaac
     3241 gcttaagcca gcatacagca acaacatggc ctggtgtctg tgggacatgc tcactcatcc
     3301 gcgctacggc atggggaaac gtcttggtgc ggcagatgtg gacaaatggg cgctgtatgt
     3361 catcggccag tactgcgacc agtcagtgac ggacggattt ggcggcacgg agccgcgcat
     3421 cacctgtaac tcttacctga ccacacagcg taaggcgtgg gatgttctca gtgatttctg
     3481 ctcggcgatg cgctgtatgc cggtatggaa cggacagatg ctgacgttcg tgcaggaccg
     3541 accgtcggat aaggtgtgga cctataaccg cagtaatgtg gtgatgccgg acgatggcgc
     3601 gccgttccgc tacagcttca gcgccctgaa ggaccgccat aatgccgttg aggtgaactg
     3661 gattgaccca gataacggct gggagacggc gacagagctt gtggaggata cgcaggccat
     3721 tgcccgttac ggtcgtaatg tcacgaagat ggatgccttt ggctgtacca gccgggggca
     3781 ggcacaccga gccgggctgt ggctgattaa aacggaactg ctggaaacgc agaccgtgga
     3841 cttcagcgtg ggcgcagaag ggcttcgcca tgtaccgggt gatgtcattg aaatctgcga
     3901 tgatgattat gccggtatca gcaccggtgg tcgcgtactg gcggtgaaca gccagacccg
     3961 gacgctgacg ctcgaccgtg aaatcacgct gccgtcctcc ggtaccacgc tgataagcct
     4021 ggttgacgga agtggcaatc cggtcagcgt ggaggtccag tccgtcaccg acggcgtgaa
     4081 ggtgaaagtg agccgggttc ctgacggcgt tgcagaatac agcgtgtggg ggctgaagct
     4141 gccgacgctg cgccagcgtc tgttccgctg cgtgagtatc cgtgagaacg acgacggcac
     4201 gtatgccatc accgccgtgc agcatgtgcc ggaaaaagag gccatcgtgg ataacggggc
     4261 acactttgac ggtgaccggc gcggcacggt gaatggtgtc acgccgccag cggtgcagca
     4321 tctgaccgcc gaagtcaccg cagacagcgg ggaatatcag gtgctggcgc gatgggacac
     4381 gccgaaggtg gtgaagggtg tgagcttcct gcttcgcctg accgtggcag cggacgacgg
     4441 cagtgagcgg ctggtcagca cagccaggac gacggaaacc acataccgct tcaggcagct
     4501 ggcgctgggg aattacagtc tgacagtccg ggcggtaaat gcccgggggc agcagggcga
     4561 tccggcgtcg gtatcgttcc ggattgcggc accggcagcg cctgtcacta ttgaactgat
     4621 accggggtat tttcagataa cggcggtccc gaaactggct gtatatgacc cgacggtgca
     4681 gtttgagttc tggttttcgg aaaagcggat tgcggatatc aggcaggttg aaaccagcgc
     4741 ccgctatctt ggcacggcgc tgtactggat agccgccagt atcaatatca ggccgggcca
     4801 tgattattat ttttacgttc gcagtgtgaa caccgttggc aaatcggcat ttgtggaggc
     4861 catcggtcgg gcgagcgatg atgcagaagg ttatctgaat ttttataaag ggttgatcaa
     4921 taaaacgcat ctcggcaagg agttgtggac gcagattgat aacggtcagc ttgcgccgga
     4981 cctgactgaa atcaggacgt ccattacgaa tgtcagcaat gaaatcacgc aaaccgtcaa
     5041 taaaaaactg gaaaatcaga gtgctgcaat ccagcagata cagaaagttc aggttgatac
     5101 aaataataac ctgaacagca tgtgggctgt gaagctgcaa cagatgaagg acggacgcct
     5161 ttatattgcg ggtatcggtg ccggtattga gaatacgcca gcaggaatgc agagtcaggt
     5221 gctgctggcg gcagacagga ttgcgatgat taatcctgcg aatggcaaca caaagccgat
     5281 gtttgttggt cagggcgatc agatattcat gaacgacgtg ttcctgaaat gcctgacggc
     5341 tccgaccatt accagcggcg gtaatcctcc ggcattttcc ctgacacctg gcggacggct
     5401 gacggcgaaa aatgccgata tcagcggtaa cgtgaacgcg aactccggga cgctcaacaa
     5461 cgtcacgatt aataagaact gtcgggttct gggaaaattg tccgcgaacc agattgaagg
     5521 cgatctcgtt aaaacagtgg gcaaaccttt cccacgggac tcccgggcac cggagaggtg
     5581 gccatcaggg accattaccg tcagggttta tgacgatcag ccgtttgatc ggcaaattgt
     5641 tattcccgcg gtggcgtttc gcggtgctaa acatgagcgg aagaataaca atatttattc
     5701 gtcatgccgc ctgatagtga agaaaaacgg tgctgaaatt tataaccgaa cgaccctgga
     5761 taatacgctg atatatacgg gtgttattga tatgcctgcc ggtcacggtc acatgacgct
     5821 ggagttttct gtatcggcat ggctggtaaa tggctggtat cccacagcaa gtatcagcga
     5881 tttgctggtt gtggtgatga agaaagccac tgcaggcatc acgattagct gaattttata
     5941 acccagatac gggcaccaga aatggtgcct tttttattgc agaaaagcga gaggtaatta
     6001 tgcgtaaagt ttgtgcagcc attttgtccg cagccatctg tctggccgta tccggtgcgc
     6061 ctgcatgggc gtctgaacat cagtccacgc tgagcgccgg gtatcttcag acccatactg
     6121 atatgccagg cagcgatgac ctgaagggca ttaacgtgaa ataccgttat gagtttacgg
     6181 acacgctggg gctgattacg tcattcagtt atgccaacgc tgaagatgag caaaaaacgc
     6241 attacagcga tacccgctgg catgaggatt ccgtgcgtaa ccgctggttc agcgtgatgg
     6301 cggggccgtc tgtgcgcgtg aatgaatggt tcagcgcgta tgcgatggcg ggcgtggctt
     6361 acagccgtgt gtcgaccttc tccggggatt atctccgcgt aactgacaac aaggggaaaa
     6421 cgcacgatgt gctgaccgga agtgatggcg gtcgccacag caacacgtct ctggcgtggg
     6481 gggctggcgt gcagtttaac ccgaccgaat ccgtggccat tgaccttgct tatgaaggtt
     6541 ccggcagtgg tgactggcgc actgacggtt tcatcgtggg tgtcggttat aagttctgat
     6601 tagccaggta acac
//
LOCUS       DAJTYZ010000186         6539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000186 DAJTYZ010000000
VERSION     DAJTYZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.186"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            100..725
                     /gene="gstB"
                     /locus_tag="OO596_003783"
                     /pseudo
     CDS             100..725
                     /gene="gstB"
                     /locus_tag="OO596_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(722..1837)
                     /gene="yliI"
                     /locus_tag="OO596_003784"
     CDS             complement(722..1837)
                     /gene="yliI"
                     /locus_tag="OO596_003784"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCR6719533.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(1948..2331)
                     /gene="bssR"
                     /locus_tag="OO596_003785"
     CDS             complement(1948..2331)
                     /gene="bssR"
                     /locus_tag="OO596_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6719534.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2544..3869
                     /gene="rimO"
                     /locus_tag="OO596_003786"
     CDS             2544..3869
                     /gene="rimO"
                     /locus_tag="OO596_003786"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6719535.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(3916..5237)
                     /locus_tag="OO596_003787"
                     /pseudo
     CDS             complement(3916..5237)
                     /locus_tag="OO596_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(5245..>6539)
                     /locus_tag="OO596_003788"
     CDS             complement(5245..>6539)
                     /locus_tag="OO596_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6719536.1"
                     /translation="IAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAV
                     GIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQ
                     TFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSE
                     FIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSA
                     ERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQ
                     LNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDD
                     FGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVES
                     EDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         1721 a         1641 c         1495 g         1682 t
ORIGIN      
        1 ctggtgtcat ttttttgcca gctgttgctt aaaagttaac tttacttcac catcggagtc
       61 ctgttcagcc tgtggctgcc tgtcggaaaa ggggtgagta tgattacgct gtggggtcgg
      121 aataattcaa ctaacgtaaa aaagtattgc tgacgctcga agaactggaa ctaccttatg
      181 agcaaattct cgcgggccgt gagtttggga taaatcacga tgctgatttt ctggcgatga
      241 accctaacgg gctggtgccg ttgttgcgtg acgacgaaag tgatctcatt ctttgggaat
      301 caaacgccat tgtccgctat ctggcggcac agtacgggca aaaacgcctg tggatcgact
      361 caccggcacg tcgtgcggaa gcggaaaaat ggatggactg ggcaaaccag acgctcagta
      421 atgctcatcg cgggatcctg atgggattag tcagaacacc accggaagag cgcgatcagg
      481 ccgccattga tgccagttgc aaagagtgcg acgccctgtt tgccctgctc gatgcggaac
      541 tggcaaaagt aaaatggttc tctggcgacg agtttggtgt gggcgatatc gctatcgcac
      601 cgttcatcta caatttgttt aacgttggcc tgacctggac accgcgtcca aatctgcaac
      661 gctggtatca gcaactcact gaacgccccg cagtgcgcaa agtggtgatg attcccgtta
      721 gctaattgcg tgggctaact ttaagtaatt ccccactgga ctcgtcggtg agaacgtata
      781 aataaccgtc gggtccagtg cgaacatcac gaattcgctg ccctctgtcc gttaaaatac
      841 ggccatcttc tgtcactttg tcgccgttga cgctcatcac aatgacatct ttatctttca
      901 gcgcgccaat aaataatttt tgctgccact gggggaattt atcgctgtta tagaaggcca
      961 tgccgctcac agcgggcgaa tctttccagt aaaaaacagg ttgctcggtc ccggcgacga
     1021 tctccccttt cgcttccggt atcttaaagc ctgaatagtt gattccccag gttgccagcg
     1081 gccagccgta gtgtttgcct ttttgcggga tattaatttc atcaccaccg cgcgggccat
     1141 gttcattcag ccacagtgca ttactccacg gattcatcgc cattccttgc ggattacgaa
     1201 tgccataaga ccagatctcg gcgcgcgcac cggattcctt tataaaagga ttatcatccg
     1261 ggatttcgcc ctggtcggtc agacgcacca gtttgccctg taatttatcc agatcctgcg
     1321 ccgtcgggcg ctgattgttt tcgcccagag caataaaaag ataaccttta ccgtcgaata
     1381 ccagccgccc gccaaaatgg ttgccggtag acagttttgg catctggcgg aagacggtgc
     1441 ggaagtcggt cacttttgag agatcatcac ttaagcggcc ataacccaca gcagttccgg
     1501 ctttgccatc atcgccaact tcggaataac ttaaccagat gcggcgagac tgagcaaaat
     1561 caggcgctaa aaccacgtcc agcaggccgc cctgcccgtg cgcccaaacg tccggaactc
     1621 cggaaagcgg cgcagataat ccttttcctg cttgccagtg acgcaactcg ccgccgcgca
     1681 gagtgattaa cataccgtga ttatcgggta aaaaggccag tgcccaggga tggtcgagtt
     1741 tgtcttgcag tacttcgaca tttaccgttg caggagccgc ccagagagcg gaagaaagac
     1801 aaataagggg cacaaggaaa aaggattgtc gatgcataac gcctccggat tgactctggc
     1861 ttaaagcgta gtcagtggag gagataacaa attcattttt acaaaaactt aaacatgaag
     1921 ggggagacgc tttctccccc ttagttttca ggccttctca agcatggcgt gcttctgcag
     1981 gctctggata ctcagcgtta agctcatcag acaattttca agcttatcgg cgttgacggt
     2041 aataacagtc gggcaatcat ggtgcccact catcaaacat actgcggctg tcgctaatgc
     2101 ttcttcagca tgatgaagag cactccactc ttcctgatcc agatgaagat tcaaccgcag
     2161 cgatttatcg tgcagttcgc gattcagttt aaaaaagtta tctcgtagat gattgctttc
     2221 gctgacggac atgtatcctt ttgccttcct cagttgcacg tatgcagcga ggtcaacgcg
     2281 tgcgtcgatc aaccggttca gcagatcgat tcgctgtctg tcaacgaaca tacttcgatc
     2341 ctcctctttc cagccttgtt gcgattaaat agtatggcta ttttttgagc aaaatgctgt
     2401 ccaggatcaa ttcttcacgc caaattaaca aaatttacat ttccttcgcc tccgccagcg
     2461 aatgagattg cgatacaaat tttatccaga actctgtaga atccctgccc tgacctttcc
     2521 tgtaactgaa tattttttaa gctatgagca aagtaactcc ccagccgaaa atcggctttg
     2581 tttcccttgg ctgtccgaaa aaccttgtcg attcagagcg tattctcacc gaactacgca
     2641 ctgaaggtta tgacgtggta ccgagctatg acgatgcgga catggtgatc gtcaacacct
     2701 gcggctttat tgacagcgcg gtacaagaat cactggaagc cattggtgaa gcgttgaatg
     2761 aaaacggcaa ggtaattgtg accggttgtc tgggggcaaa agaagatcag atccgcgaag
     2821 tccacccgaa agtgctggaa atcaccgggc ctcatagcta tgagcaggtt ctggagcacg
     2881 ttcatcacta cgtgccaaaa ccgaaacaca acccattcct gagcctggtg ccagaacaag
     2941 gtgtgaaact gacaccgcgt cattatgcct atctgaaaat ttcggaaggc tgtaatcacc
     3001 gctgcacctt ctgcattatt ccgtctatgc gcggcgacct ggtgagccgt ccgattggcg
     3061 aagtgttaag tgaagcgaaa cgtctggtag atgcgggcgt taaagagatt ctggtgatct
     3121 cgcaggatac ttccgcctat ggcgttgatg ttaaacatcg tactggcttc cacaacggcg
     3181 agccggtaaa aaccagcatg gtcagcctgt gcgaacagtt atcgaaactg gggatctgga
     3241 cacgtctgca ctacgtttac ccttatccgc atgtggacga cgtcatccca ctgatggcag
     3301 aaggcaaaat cctgccgtat ctggacattc cgttgcagca cgccagcccg cgcattctca
     3361 aactgatgaa gcgtccgggt tctgtagatc gccaactggc gcgcatcaaa cagtggcgcg
     3421 aaatctgccc ggaactgacc ctacgctcaa cctttattgt cggcttccct agcgagacgg
     3481 aagaagattt ccagatgcta ctcgacttcc tgaaagaagc gcgtctggat cgcgttggct
     3541 gctttaaata cagcccggtt gaaggtgcag acgccaatgc cctgcctgac caggttccgg
     3601 aagaagtgaa agaagaacgc tggaaccgtt tcatgcagct gcagcagcag atttccgccg
     3661 agcgcctgca agagaaagtg ggccgtgaaa ttctggtgat tatcgacgaa gtggacgaag
     3721 aaggcgcgat tggtcgcagc atggcagatg caccggaaat cgacggcgcg gtttatctca
     3781 atggtgaaac caacgttaag ccgggtgata tcctgcgtgt gaaagtcgag cacgccgatg
     3841 agtacgattt gtggggtagc cgggtttaat taattcaggc cgggcaaaat catttgtccg
     3901 ccctgcgaat aatgattact gccagctaat ctgagccctg ccgtttttct tactcttata
     3961 cagccgctca tccgccagca ttttcaagcg ttcgacggtg gtacattcat ttgaatgcgc
     4021 tacaccgata ctggtactta aattaatgct tcccacctgg aaatgaacga tttcttttag
     4081 aatcgatagc aactcttcca gcgttccacc cgtataaatc accgcaaatt cgtcaccgcc
     4141 gaagcggtag attttacctt tatctaccgg gatctgtgat ttaaggattt tcaccacttt
     4201 catcaacact tcatcgccca gcaaatgacc gaaggtgtcg ttaatgtttt tgaatttatc
     4261 gacgtcaaac aggcacaaat aaccgccttt ttccggcagc gcttttaaat cttcgtcaaa
     4321 ggattttctg cccattgctt tggtcaggtc gtccaggaac gccgagttat acatactgta
     4381 gagttttttg acatcggcag ccgaaaagaa aagcccgcca tccagtacgc gttcaaaatg
     4441 gttattaatg atggcttctt tctccacaat accacgatta atcactctgc gtaccagata
     4501 gaggcaactg gtacccgata tcactgccac caatgcggca aagaaaataa tcaatgtaca
     4561 gacccgcaaa taaacatctt ttttatacgc aaatacgtac aatccggtcg gcaaataggc
     4621 gaagtgctcc atcgcttccg aaacggtgaa gatggcgttt ttagcattat attcatcacc
     4681 ggtttaccgt tcttaaatag agagaacatc aggttctcgc tgtccatcgc cagcgatttt
     4741 gacagtaaag aaaaacgctc cagaccaagc aaaaatatca gactacgatt gtgagttacc
     4801 ggatagaggt aatagacgaa ggttttaccg tcaaaagaga taaaccctgt atttttgaca
     4861 tgtcgatact tcacaacctg cataatatct gcacaataat ttttctcgaa ctcctttacc
     4921 tgaatacatt cgtcactgga aatcagataa ggaccgacat cgtacaaata agagggggaa
     4981 accgagtcgg cgtaagctgt gcgcaaacca accaacagct gttcctccgt gggatttagc
     5041 gtgttgataa gatcaattgc cacgctacgc cgatattcag caatatttgc caccgcaaaa
     5101 gcagtatcaa taacggactg tttcaggcta tacacccgtt cctttaccat ttgagtgtag
     5161 atcccgcagg caactgctga aatcatgata aaaatcagca gactgactgt aagtacgaac
     5221 ttattgattc tggacatacg taaactactc ttttactaat tttccacttt tatcccaagc
     5281 ggagaatggc atgggtttct ggtacagata cccctgagcc tgaacacatc ctaatttaat
     5341 taattcgcgc gcaacgtctt cgctctctac gccttccgca atcaccggga tccggttata
     5401 gcgtgccaga tcgaaaataa tgcgcacaat ttttcccgca gtaccgaaaa tatcgccggt
     5461 aaattgcgag tccaccttta tgctgtctgg attaatttca taaaaacgtt tcaggtttga
     5521 caaaccggtg ccaaagtcat caatcgatat ccgtacccct tgtttacgca gttgttcaat
     5581 attgagataa aaatgttcat taattcgctc gatcacggtt tcagtgatct caacacagag
     5641 gctgttcgcc ttcaggccat tttctttcat gatggtggta aaacgctcat gaaagtgcgg
     5701 ttcgtttaac tgccagggcg aaacattaat atgcagtaag aaatcatcct tcagcgacgc
     5761 acgctgacta aaatgtctga attcacccat tgccgtttta ataatctgat aacccagctc
     5821 attgataaac ccgatatctt cagcaatggt aatgaacttc agcggtgaaa tgatcccccg
     5881 ctccgcagaa acccaacggc acaacgcctc cgcgcccaga atggcgcggg tgttaatatc
     5941 aacgatcggt tgatagacga ggaatagttc gccaatttcc attgcatggc gaatattacg
     6001 cgcaatccag atattctctt ctatttcgtt gagtttcgcg tcatcacaga aaataaactc
     6061 ggagaacata tggtcacgga tactggaaat cgccagtttc agtttttctg cgtaactcca
     6121 ggcgttttcc ccctgcaaag gttcacaaat ggcagactta tggatgcgca gattgcggta
     6181 gccaaagctg ctgtcaaaat cgtttaccat cgctatttta cgggtaaatg tctgtacatt
     6241 ttcccgacac agaagtaaat agagatcggc attgaatgtc acgatatcgc agcattgcgc
     6301 atatttctcg gcaattcgct gacacaattt cctgacgatt ttattgcgtt catcaacacc
     6361 aaacaccgct tccacggctt ccagaccaca cagtttgatg atccccacag ccagatactg
     6421 attattgttg gactcaataa tcgccatatt tttaataaaa ccgctttcct gatcgtaaga
     6481 gatcagtgag agcaatttat ttgaccgttc aatttcggca tctttcattt ccgcgatcg
//
LOCUS       DAJTYZ010000187         6527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000187 DAJTYZ010000000
VERSION     DAJTYZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.187"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="OO596_003789"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="OO596_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6719537.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="OO596_003790"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="OO596_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6719538.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="OO596_003791"
     CDS             2408..3157
                     /locus_tag="OO596_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6719539.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="OO596_003792"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="OO596_003792"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OO596_003793"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OO596_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6719540.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="OO596_003794"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="OO596_003794"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6719541.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="OO596_003795"
     CDS             6018..6491
                     /locus_tag="OO596_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR6719542.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1604 a         1649 c         1649 g         1625 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcaga
//
LOCUS       DAJTYZ010000188         6496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000188 DAJTYZ010000000
VERSION     DAJTYZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.188"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1161)
                     /locus_tag="OO596_003796"
     CDS             complement(<1..1161)
                     /locus_tag="OO596_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6719543.1"
                     /translation="MLIYLYPVLLLFNILPVFFYGQMNSDLERFFGVPIGYIPDLIFY
                     FFVVLTSIITLRFHVSLWTKKLLFLGIIFLIYISIQMLLLSADISGVVILLSFFSNFI
                     ALVLLVSFCIGKDELYLTHSVRNINVVMCFGIICGVVKLFIGYSEDSNFIVYLNRNAT
                     AIIVVCFYCVYSYFYRGRKSWYVSSVLYSLFFLFLDSRAGIISFAISLFFVFLQLTKK
                     EKLLISLFFVPLLTLGISFTDIGTRLERMLSSSQVIFSGGNTLTKSQNDYRRVELVFI
                     GVDVLKENYLIGTGLGVANYVKAIDKKFLGSTNFGLAHNFYLSYSAQLGIIGFILLIS
                     VFYIMLSPIFKCGGYIGKGCVFALAFYVFFNEYILTPAIYIYISIFLSVVFIR"
     gene            complement(1230..2510)
                     /locus_tag="OO596_003797"
     CDS             complement(1230..2510)
                     /locus_tag="OO596_003797"
                     /inference="COORDINATES: protein motif:HMM:NF014051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR6719544.1"
                     /translation="MFRKMIDAGGTFLLKAIFQIGVFVYFTHVSDITTFGIISYVFTV
                     YWFVLNFSDYGFRTKLVKDISDNSYSASELLSRSDGVKTYVFFFIFIIFMFYSYVSDS
                     ISLTLLVYISSAYFVCISSGRFSLLQAVGRFRCELYINIYSTIIYIGCNLFLSLFIEP
                     LYYSAISIFIYSISLLVFSSHKCNVPCFHIKRPSILVYKDFLDATPFAILVLLNVVLS
                     SIDLFILKEYFSYNSVAIYQVVTRVNTGLIIVFNVIYTVLLPSFSYYLKNSEWGNIRK
                     LQRYISLLVLLLCLCYYFFGIYFVGILFGDEYKVISSATFLIMFMALIKYNFWLINEL
                     YLVCSGNQSERVKSYCIGVVISMAVFFYFIPRYGWSGAVFGSAIATLVIGIFYIISVK
                     KDCGKILHDKYSLMMIFVPIFFYFIINGQQRLLY"
     gene            complement(2599..3624)
                     /gene="tviC"
                     /locus_tag="OO596_003798"
     CDS             complement(2599..3624)
                     /gene="tviC"
                     /locus_tag="OO596_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosaminuronic acid C-4 epimerase TviC"
                     /protein_id="HCR6719545.1"
                     /translation="MSRYEEITQQLIFSPKTWLITGVAGFIGSNLLEKLLKLNQVVIG
                     LDNFSTGHQYNLDEVKTLVSTEQWSRFCFIEGDIRDLTTCEQVMKGVDHVLHQAALGS
                     VPRSIVDPITTNATNITGFLNILHAAKNAQVQSFTYAASSSTYGDHPALPKVEENIGN
                     PLSPYAVTKYVNEIYAQVYARTYGFKTIGLRYFNVFGRRQDPNGAYAAVIPKWTAAML
                     KGDDVYINGDGETSRDFCYIDNVIQMNILSALAKDSAKDNIYNVAVGDRTTLNELSGY
                     IYDELNLIHHIDKLSIKYREFRSGDVRHSQADVTKAIDLLKYRPNIKIREGLRLSMPW
                     YVRFLKG"
     gene            complement(3717..4997)
                     /gene="tviB"
                     /locus_tag="OO596_003799"
     CDS             complement(3717..4997)
                     /gene="tviB"
                     /locus_tag="OO596_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HCR6719546.1"
                     /translation="MKFDTLNAKIGIIGLGYVGLPLAVEFGKKVTTIGFDINKSRIDE
                     LRNGHDSTLECSNLELLEATKLTYACSLDALKECNVFIVTVPTPIDKHKQPDLTPLIK
                     ASETLGKIIKKGDVIIYESTVYPGATEEDCIPVVEKVSGLKFNIDFFAGYSPERINPG
                     DKEHRVTNILKVTSGSTPDVAEYVDQLYKLIITVGTHKASSIKVAEAAKVIENTQRDV
                     NIALINELSIIFNKLGIDTLEVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQS
                     VGYHPEMILAGRRLNDSMGQYVVSQLVKKMLKQRIQVEGANVLVMGLTFKENCPDLRN
                     TKVIDIISELKEYNINIDIIDPWCSTDEAQHEYGLTLCEDPKVNHYDAIIIAVAHNEF
                     REMGESAIRALGKDEHVLFDLKYVLDKKSIDMRL"
     gene            complement(5066..6172)
                     /locus_tag="OO596_003800"
     CDS             complement(5066..6172)
                     /locus_tag="OO596_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6719547.1"
                     /translation="MSKASEPQQTPYLIPQGVYPTYMPKAEDEIDLFELLGTLWKKKW
                     VILCVTLLTTGLAAVYAFTAKEQWTAKTYIQAPRIAELGSYLKFHQAYARILNQPLDT
                     NALANGLFSDLILIAESPDTKVKFLESTEYYKKETNNLSTEQDKKIWLAEQANKGLVI
                     TPPKEKGNTSYYIIQASADSAQEAYKLLQGYLKNVNNQAVTLSLDEFGQNVNTLLVNL
                     NKEIIDIDFQRKSEKLDQIAHIQRDLTTAEQAGIIDYRSSKGGFDNAQSSYKFLLGEK
                     LLSAELKATKDAPIIYPFRYYEVKRQIDELEGMLRDNIQAQAYRYQMKPSEPVIKDKP
                     NKALILILGALPGAMFAIVGTLVYATLKDKTKLD"
BASE COUNT         2354 a         1256 c          913 g         1973 t
ORIGIN      
        1 acgtataaaa accaccgata aaaaaataga aatataaata tatatcgctg gcgtcaatat
       61 atactcatta aaaaagacat agaaagccaa agcaaaaacg catcctttac caatataccc
      121 tccgcattta aaaattggag acagcattat ataaaataca gaaataagca aaataaaacc
      181 aataatccct aactgagccg aataagataa ataaaaatta tgcgccaacc caaagttggt
      241 acttcctaaa aactttttat ctatagcctt tacataattt gcaacaccta atccagtgcc
      301 aattaaataa ttttctttta aaacatcaac cccaataaat actaactcaa ctcgacgata
      361 atcattctga ctttttgtaa gagtgttacc accagagaat ataacctgtg acgaagacag
      421 cattcgttca agacgagtgc ctatatcagt aaaagaaata cctaaagtta gaagaggaac
      481 aaaaaacaat gatattaata acttttcctt ctttgttaac tgaagaaaaa caaaaaacaa
      541 cgatatagca aatgatatta ttcctgctcg gctatccaga aaaagaaaga acagagagta
      601 caatacagat gagacatacc aagactttcg accacgataa aagtatgaat atacacaata
      661 aaagcacact actataattg cggtggcatt tctatttaaa taaactataa aattactatc
      721 ttcagaataa ccaataaata attttacaac tccacagata ataccaaaac acattacaac
      781 atttatattt ctaaccgaat gagttaaata aagctcatct ttaccaatgc aaaatgacac
      841 caaaagaacc aaagctataa aattagaaaa aaacgataat aaaattacga cacctgatat
      901 atccgctgat aacaacaaca tctgaatgct gatataaatc aggaatatga tgcctaaaaa
      961 taataatttc tttgtccaca gagaaacgtg aaacctcaac gttattatag atgttaaaac
     1021 aacaaagaaa taaaatatta gatctggaat atagccaata ggaactccaa aaaaacgctc
     1081 taaatcagag ttcatttgtc cataaaaaaa aaccggaagg atattaaata acaaaagtac
     1141 aggatatagg tatattaaca tacttaacct ttattaggct tccaatcgag tctaaacata
     1201 ttaatggaac gatataaaac cacaacatat taatataaca accgctgctg accattaata
     1261 ataaaataaa agaaaattgg gacaaagatc atcattagtg aatacttatc atgaagaatt
     1321 ttcccacaat cttttttcac agaaataata taaaatattc caattactaa tgttgcaatg
     1381 gcacttccaa aaaccgcccc actccatcca taccgaggta taaaataaaa gaaaaccgcc
     1441 attgaaatga ccacaccaat acaatacgat ttaactcgct cgctttgatt tccactacac
     1501 acaagataaa gttcatttat tagccaaaaa ttatatttaa taagagccat aaacattatc
     1561 aaaaatgttg cagaagatat taccttatac tcatcaccaa acaatatccc tacgaaatag
     1621 atgccaaaaa aataatagca taaacatagt aataagacta acagtgatat atatcgttgt
     1681 aatttcctta tattacccca ttcagaattt ttcagataat aagaaaatga aggcaataaa
     1741 acagtataaa taacattaaa cactattatt agaccggtat taaccctagt taccacctga
     1801 tatatagcaa cactattata agagaaatat tcttttaata taaaaaggtc aatactagat
     1861 aaaacaacat ttagtaacac cagaatagcg aacggagttg catccaaaaa atctttataa
     1921 actaaaatac ttggtctttt tatatgaaaa catggcacat tgcatttatg tgatgagaaa
     1981 accaaaagcg aaattgagta tatgaatatt gatatcgcac tataatatag aggttcgata
     2041 aacagagata aaaataaatt acacccaata tatataattg ttgagtagat atttatatat
     2101 aattcacatc taaaccgacc aacagcctgt agcaagctaa atctaccact tgaaatacaa
     2161 acaaaatatg cagatgaaat ataaacaagc agagttaatg aaattgaatc agaaacataa
     2221 gaataaaaca tgaagattat aaaaatgaag aaaaaaacat atgttttaac tccatcactt
     2281 cttgataata attctgatgc agaataacta ttatcagaaa tatctttcac taattttgtt
     2341 ctaaatccat aatcagagaa gttaagcaca aaccaataaa cagtaaacac ataactaata
     2401 ataccaaatg tagtaatatc tgacacatgt gtgaaataaa caaaaactcc tatttgaaat
     2461 attgctttaa gtaaaaatgt accacctgca tcaatcattt ttctaaacat acactgacca
     2521 tttagaataa atacatggat tttcacccct gcattaataa cagaggtgaa atagattatt
     2581 tattcatgtt aatataattt agccttttaa aaatctcaca taccacggca ttgaaagtcg
     2641 taatccctct ctgattttta tatttggtct atactttagt aaatctatag ccttagtaac
     2701 atcagcctga gaatgcctaa catctccaga tctaaactct ctatacttaa tgctcaattt
     2761 atcgatatgg tgaattaaat taagctcatc ataaatgtaa ccagataatt catttaacgt
     2821 tgttctatca ccaactgcaa cattatatat attatcttta gcactgtcct tcgctaatgc
     2881 agataatata ttcatttgta taacattatc tatataacaa aaatcacgac tcgtttcacc
     2941 atcgccatta atatatacgt catcaccttt aagcattgct gctgtccatt ttggaattac
     3001 tgcagcataa gctccattag gatcttgacg acgaccaaat acattaaaat aacgtaatcc
     3061 aatagtttta aaaccatatg ttcgagcata tacctgagca taaatctcgt taacatattt
     3121 agtaactgca taaggagaaa gtggattacc aatgttttcc tctacttttg gtagtgcggg
     3181 atgatctcca taagttgagc ttgatgcagc ataagtaaaa ctttgtactt gtgcattttt
     3241 agccgcatgt aagatattca aaaatccagt aatattagtt gcattggttg ttataggatc
     3301 aacaattgaa cgaggtacag aacctagcgc agcctgatgt aagacatgat caacaccttt
     3361 cataacttgc tcacaggtag tgagatctcg aatatcacct tctataaagc aaaatcgact
     3421 ccactgttca gtggaaacta atgttttaac ttcatcaaga ttatattgat gtcccgtgga
     3481 aaagttatct aacccaataa caacctggtt taatttaagt aacttttcta aaagatttga
     3541 tcctataaag ccagcgacac cagtaattaa ccaagttttc ggtgaaaaaa ttaactgctg
     3601 tgtaatctct tcatagcgag acatataaat atccatgtat attaatcaag tgctaaacct
     3661 agcataaaat gctgaggtgt atcatatcaa agaggatttg atttttttaa tcactcttac
     3721 aagcgcatat cgatactttt tttatcaagc acatatttta aatcgaacaa aacgtgctcg
     3781 tctttaccta atgcacgaat agcgctctct cccatctcgc gaaactcatt gtgtgcaaca
     3841 gcgataatta ttgcatcata atgattaact ttaggatctt cacataaagt taatccatat
     3901 tcatgttgtg cctcatcggt agaacaccat ggatctataa tatctatatt gatattgtat
     3961 tcttttaact ctgaaataat atcaatcact ttagtgtttc gtagatctgg gcaattctct
     4021 ttaaatgtaa gccccatcac taacacattc gccccttcaa cttgaatccg ttgtttcaac
     4081 atttttttga ctaactggga aacgacatac tgccccatac tatcatttaa acgacgtccg
     4141 gctaaaatca tctccggatg atagccgaca ctttgcgctt tatgtgtaag ataataagga
     4201 tctacaccta tacagtggcc acctactaaa ccgggcctaa aaggtaaaaa attccacttc
     4261 gtacctgcag cctcaagaac ctctaaggta tcaatcccta acttattaaa tataatagat
     4321 aactcattaa tcaatgcaat attgacatct cgctgcgtgt tttcaattac ttttgcagcc
     4381 tctgctactt ttatcgatga tgctttatgc gtaccgacag taattattaa tttatatagc
     4441 tgatctacat actcagcaac atccggtgta gatccactgg tcaccttaag gatattagtt
     4501 acacgatgct ctttatcccc aggattaata cgctcaggtg aataaccggc aaaaaaatca
     4561 atattaaact taagacctga tactttctct acaactggta tacaatcttc ttctgtcgct
     4621 ccagggtaaa ctgttgactc ataaataata acatcgcctt tctttattat cttacccaat
     4681 gtttcagatg ctttaattag aggtgttaga tctggctgtt tatgtttatc aattggagtt
     4741 ggaacagtta caataaatac attacactct tttagtgcat ctaatgaaca ggcgtacgtc
     4801 aatttagttg cttctaacaa ctctaaattt gagcactcta atgtactatc gtgaccattt
     4861 cgtaattcat caatacgaga cttattaata tcaaatccaa tcgtcgttac tttctttcca
     4921 aactcaacag caagaggcaa tccaacataa ccaaggccta taatcccaat tttcgcattc
     4981 aaagtatcga atttcatttt cttatccatc ataaagtgca catagaacat ctatttcgca
     5041 ttgacacaac aatacgtaac ccagtttaat ctaacttggt tttatctttt aatgtcgcat
     5101 aaactaatgt accaactata gcaaacattg cccctggtaa tgcaccaaga atcaaaatta
     5161 atgctttgtt gggtttgtct tttataactg gctcagatgg cttcatttga tatcgatatg
     5221 cttgcgcctg aatgttatcg cgtaacattc cttctaactc atcaatttga cgtttcactt
     5281 cgtaatatct aaatgggtaa ataattggcg catctttagt tgcttttagc tctgctgata
     5341 acagtttttc gccgagcaag aacttatagc tactttgcgc attatcgaag ccgcctttgc
     5401 tagagcgata atcaatgatt ccggcttgtt ccgcagttgt taaatctcgc tgaatatgag
     5461 ctatttgatc aagcttttct gattttctct ggaaatctat gtcaataatt tctttattta
     5521 gattaaccaa aagagtatta acattttgac caaactcatc aagacttaat gttacagctt
     5581 gattattaac attctttaga tatccctgca gtagtttata tgcctcttgc gctgagtctg
     5641 ccgatgcttg tattatgtag taacttgtat ttcccttttc ctttggtggc gtaatcacaa
     5701 gacctttatt cgcttgctca gctaaccaaa ttttcttatc ttgttcagta gataaattat
     5761 ttgtttcctt tttataatac tcagtactct ctagaaattt aactttggtg tctggcgatt
     5821 cagcaatcaa aatcaaatcg gaaaacaatc cattagccaa cgcattcgta tctaacggtt
     5881 gatttaatat tcgggcatac gcttggtgaa atttaagata gctgcctaat tcagcaatac
     5941 gtggtgcttg aatataggtt ttagccgtcc actgctcttt tgcggtaaag gcatacactg
     6001 ctgctaaccc cgtagtcagc aacgtgacac ataaaataac ccattttttc ttccacaagg
     6061 tgcctaaaag ctcgaaaaga tcgatttcat cctctgcttt tggcatataa gttggataga
     6121 ccccttgcgg gatcagataa ggggtctgtt gtggttcaga tgcttttgac atgaaaccaa
     6181 tccattattt atcaatgggt taaaaataaa aatgaagaat aagcctgaca aaaaagtacg
     6241 ctaccgccct tgagtttcag ctcccaaagc gctgcgcaga aacaggatat agccaactgc
     6301 gatacaacaa gtgatatctg ggattagagg acataatcga gaacactccg tgtgaagaga
     6361 catatacagt cccacacttc cctgctgcaa accggcggat agcccgcaga atttggagtg
     6421 cagattataa agcaatgcct gtataagacc aaatctgcct tcaaaatgat aacagtatgt
     6481 ttgtaaattc tgtgag
//
LOCUS       DAJTYZ010000189         6426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000189 DAJTYZ010000000
VERSION     DAJTYZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.189"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(47..397)
                     /locus_tag="OO596_003801"
     CDS             complement(47..397)
                     /locus_tag="OO596_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6719548.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(440..904)
                     /locus_tag="OO596_003802"
     CDS             complement(440..904)
                     /locus_tag="OO596_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6719549.1"
                     /translation="MSRVTAIISALVICIIVRLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(901..1398)
                     /gene="rrrD"
                     /locus_tag="OO596_003803"
     CDS             complement(901..1398)
                     /gene="rrrD"
                     /locus_tag="OO596_003803"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCR6719550.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(1398..1613)
                     /locus_tag="OO596_003804"
     CDS             complement(1398..1613)
                     /locus_tag="OO596_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6719551.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(2294..2368)
                     /locus_tag="OO596_003805"
     tRNA            complement(2294..2368)
                     /locus_tag="OO596_003805"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2333..2335),aa:Gly,seq:tcc)
     gene            complement(2372..2447)
                     /locus_tag="OO596_003806"
     tRNA            complement(2372..2447)
                     /locus_tag="OO596_003806"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2412..2414),aa:Thr,seq:tgt)
     gene            complement(2450..2526)
                     /locus_tag="OO596_003807"
     tRNA            complement(2450..2526)
                     /locus_tag="OO596_003807"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2490..2492),aa:Arg,seq:tct)
     gene            complement(2528..2604)
                     /locus_tag="OO596_003808"
     tRNA            complement(2528..2604)
                     /locus_tag="OO596_003808"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2568..2570),aa:Ile,seq:cat)
     gene            complement(2794..3357)
                     /locus_tag="OO596_003809"
     CDS             complement(2794..3357)
                     /locus_tag="OO596_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6719552.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(3461..3679)
                     /locus_tag="OO596_003810"
     CDS             complement(3461..3679)
                     /locus_tag="OO596_003810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719553.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(3838..4224)
                     /locus_tag="OO596_003811"
     CDS             complement(3838..4224)
                     /locus_tag="OO596_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6719554.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(4214..4585)
                     /locus_tag="OO596_003812"
     CDS             complement(4214..4585)
                     /locus_tag="OO596_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6719555.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(4585..4842)
                     /locus_tag="OO596_003813"
     CDS             complement(4585..4842)
                     /locus_tag="OO596_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719556.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(4839..6230)
                     /locus_tag="OO596_003814"
     CDS             complement(4839..6230)
                     /locus_tag="OO596_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6719557.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(6227..6334)
                     /locus_tag="OO596_003815"
                     /pseudo
     CDS             complement(6227..6334)
                     /locus_tag="OO596_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT         1537 a         1769 c         1459 g         1661 t
ORIGIN      
        1 ggtcacaggg atttgccccg cccctcccta caagtgagaa taattatcac cggattcgtt
       61 cgcgcgctgt tttcgcttta tggcagggcc agcacagact ctgcaggttg ctgtctgcgt
      121 ctgttccgcc atgcgctttc gggatgatgt ggtcgacggt tttcgcctcg ctcaccacac
      181 cgacacgcag acacaactga cacagacctt tatcgcgctt cagaatacgg gcacgaatca
      241 ccgtccattt tgagccatag ccacgctggt ggcggctcag tccgcgctgg tgctgcaccc
      301 agccttcacc gcgatgttta tcgcagtaac ccgaactgtc tgtggttgta cctgcacatc
      361 cacgcttacg gcaggcacgt gggattagtg atggcataaa tacctcatac cctgcgaaat
      421 gtttaccacg ataaaaaggc tacttaatgc actgagtgcg gatatactcc tgtgcccctt
      481 ccagttgcat ctgcatcgtc atcagccgct ctctgagggt gaaataatcc cgttcagcgg
      541 tgtctgccag tcgggggctg gttgcattat ccacgccgga ggcggtggtg gcttcacgca
      601 ctgactgaca gactgctttg atgtgcaacc gacgacgacc agcggcaaca tcagcgcgca
      661 gagtttcatt ttcagctttc gcatcagcta actccttcgt gtatttagca tcgagtgcat
      721 taacatcacg ctgtctctgc tgcatgtcag taatggttgc gttcgccagc ttcagttctc
      781 ttgcgttttt gtcgcgctgc tctttgtagg taatggcgtt atcacggtaa tgattaacag
      841 cccatgacag gcggacgatg atgcagataa ccagagcgga gataatcgcg gtgactctgc
      901 tcattgctgc ccccacaaac agacttcacg ctcaatctca cgacgggtca tcagcccttt
      961 ccattgctta ccgccagcat atgtccagcg acgtagctgg tcacatgcgc ctttgatatc
     1021 gccctggttt attttgcgaa gaagcgtcga tgttctgaaa ttgccagcgc ccacgttgta
     1081 aacgaacgag taaagagcgc cgcgcgttgt ttccggtata tcgactttga tgtacgggtt
     1141 aatttgtctg gcgacagtgg caaggtcttt attcaggagg gctttgcatt ctgcttcggt
     1201 atacgtttta ccgagcatga tgtcttttcc ggtgtgtccg tgacatacag tccatacacc
     1261 aatgatatct ttgtatggta tgtagctgac accttccaga ccatcgttac cacctgggcc
     1321 agtgattaac acagatgcta tagcaacagc cccaccacca atagcagctg caacagcctt
     1381 gcgtaatgat ggcgacatta ttcacctctc gcagccttac gcttgtcttc tcttattttg
     1441 aaatacagat ttgtcagata agtcagaagc cccagaagca gacttcccag cacaccaatc
     1501 gcagcccact gtgatggact gacctgatcc aaccactgca aaaaccagta gccggcactg
     1561 ccggcggagg tgccgtaggc aatgcctgtt gatatttttt ccatctgata catatcccgc
     1621 cccccgacag acctgtgcta tcggaaagaa aaaaggccat cagcaaaact ctgatggcct
     1681 gaatcacctt taccaatatt gtatgaaaaa acacgcacga ctaattgaca ataattttca
     1741 tttccattaa aaaagatcgc gtagcattct taattcatga ggaactttac ccgccagcaa
     1801 tctgagtagc gaaagctgtt catccccaat gtttttgctg gcgggtcctt ttttctacgg
     1861 tcctcctccc cggagggggg catttcatta tttttctaat cgttactggt gagaaggcgg
     1921 aagaaaaagc cagttcttcg cccctaaata acagcatgat tccagtccat gcaaaatctg
     1981 atttccagct cacacatacc gagcgctgta caaaaaaccg ccagccaagg cagttaagag
     2041 tgtgttcccg gggtttgctt agaatatttt gtaagttgtc cgaagagata tttacaacac
     2101 cagaatgatg aatcatcagt cccctgccag aggttcacta cgtaacctga ccgacaatgc
     2161 accattctgg tgctgaaaaa agagcactgg aactgcaaca aaacaacatc acagaacagg
     2221 agatggtaag gagtcagaca ttcccacaca atattgtgtc agtgcaaata acaaccttcg
     2281 tctcaaatct cgctggagcg ggcagcggga atcgaacccg catcatcagc ttggaaggct
     2341 gaggtaatag ccattatacg atgcccgcat atggtgccga ctaccggaat cgaactggtg
     2401 acctactgat tacaagtcag ttgctctgcc tgctgagcta agtcggcgct ggcccaccac
     2461 cgaggactcg aacctcgcac cgtcaactta gaaggttgat gctctatccg gatgagctag
     2521 tggtggttgg tggcccttgc tggacttgaa ccagcgacct ggcgattatg agtcgctcgc
     2581 tctgaccaac tgagctaaag ggccggaggc agaataataa ccatatgtca tcacatctgc
     2641 aaactcatct gaccaccagc acgtttaacg tcctgtgccg tttttcaggt ataaaaaaac
     2701 ccgctcatcg gcgggtttaa gctgtgtggc gtagtaacca ctcttaacag gatattcaac
     2761 tttttacgat cgtaaagttt tcggaaaaaa tttttaaaac cgcatcaacc gctccaccag
     2821 ttgctcttta cgggcaacga tccacccgtg ttgctccaga taaaatttaa accgttccag
     2881 agtacatacc atcgcatcgg cgggtacctt ttccgtgaac tcaacctgac cgtgtttatc
     2941 gaagtggctc agtaatgcgc atccatcatt ttcgatagat gtgttttgtg ccgctggtgg
     3001 ctgtttctgg ttgaaataac agtcttccag tttttcgaac acttcccacg cctgatcggt
     3061 ttcgagcatt ttggcgtgac gggctgcgcc acgttctgtc cagaggatga gggagcgaac
     3121 gttacgggca attttcacag aaactcttaa agagttttcg ttcttcaact ctcgtaacgc
     3181 atcaccttct atcaggaaaa aatgtttacc ttctataaac cgttctttgt ttcgattgaa
     3241 attgtttgtc aaacgctggc gctctgttcc gtacaaatca gccagcagct ctgttgtcat
     3301 gacaggaaga tggttatgcg taagtgacgg gagtttttca atagacgtgc gattcataga
     3361 catgtccttt cgaatatttt gatttacccc ttttgagagg gtgaccgggc gctcaaaacc
     3421 gtcgaaagtc ggcgggcgta tttccattgc tggtattgta ttagccgcac acccggtcat
     3481 aaaccaagaa ttctggacac aaaaaaacca catggctatc gggtgtggat accgctttcg
     3541 aggtgttttg agcaccgcgg ggaaatgtaa actgcaaacc tgaaactgtc aacaccgtca
     3601 ggttccaatc atcgggtggt gagacgcaca gggttggaac taccgggaaa ccgaccggcg
     3661 agcttttcag ctcccccatg cgccccacca taattcagat gtgcgcgtgc atacgacaat
     3721 aaaaaacacg ctcgcggcgt gtgtctgtcg cggtctctat ccggggttcc aatcccgacg
     3781 ccagattttg ctggcgcgtg aggaatatag ccccggacaa tgtgtctggt caagcaccta
     3841 cataattcgt tctacgtatc tgtccatctc cagccggata tcaagcatca tcaacatgcc
     3901 atcaataacc ccttccgctt tctgcaggcg cttgccaata caggtatccg aacacccatg
     3961 tttccgtgcc agactcataa aagtcattcc acctacgtaa taatccacca gcaaatcgtg
     4021 caaatcctga tttttcttgt tcaaccgggc catacagcca caaattatca ttgcatcatc
     4081 atcagaacac tgagggcgtg atttcacttt cgacggaatt aaccctttaa aaccagcagc
     4141 gatcgacgcc caggatacat cttcatgagt gtttgcagcc caagcccccc accgttccat
     4201 aacctgctga atatcacgcg ccatcgttat cacctgtgat ttcataaatc ttcacgccca
     4261 gtcgcccacc aggaacgagc tgaccgcgca caatattgat ttcatcaaac tgctcgtcgt
     4321 ctatgagtag tccggcatgc gtcagcgcat ccagtggtgc tttcaggata ttgtccaggt
     4381 cacgacggcg cttatccggt ggctctgcaa taatctttat cgccagcctt ccggacaggt
     4441 ttaatttcag ccgctgctgg cgaacaatta gcgtcacatc acggcgataa cgctcaccaa
     4501 cttttgatac aaaatatgtg ctgccacgac gtcgccagta agtattcacc gtcggcggat
     4561 aaggtaaaat aaactcatgg cgcatcagcg cagcacctcc tgcaccattt tttcaaactt
     4621 tccaactttg gtttccagct ctgccacaca atcaacgagc tcatccaccg ctttttgtgc
     4681 gcgatgtttc gcctgcatca gttccctgag cgctggcacc atatcccgac gaatggcatc
     4741 ttttgttaca cctgtttttt ccagttgttc cgcctgtcgc aacatttcct gcgcgtgttt
     4801 acgcaattgt tcagaggtaa aagtcattgt ctggttgttc aaaagaaacg ctccatctta
     4861 ctgctgtcgg tacgtgtatt gctgtatctg cgcggctggg gctgccgcat tggggtggaa
     4921 agaacctgtg cgctttcctg gtccacaggc agaaaatgtc cgttataaaa acgccggtaa
     4981 atcgtcccca gagaaccgtt acgttgtttc gtgatattaa tttctgctat gcctgtagcc
     5041 tgtgtatccg ggttgtacac ttcatccctg taaagcatca gaatgatgtc tgcatccgcc
     5101 tctatttctc cggaattttt caggtctgag ttcatgggac gtttattggg tctggactcc
     5161 acaccgcggg agagctggct cagcgcaatc aacggaaaac caccggattt tgccaggcct
     5221 ttaagcccct ttgagatttc acccacggca aggtcatgac gccccgtggt tcgggttttt
     5281 atcagcccga gataatcaac caccaccagc gccgtttccg gatgtttaat cagatggtgt
     5341 ttcgttgttg cgcatatctc atcaatggtc aggttcgcct ggtccaccat ccagatattg
     5401 cgcccggtca tccgccccac cccttgtgag aaacgcgccc agtcttcatc ttcaaagtga
     5461 gccacagatt tcaggcgtga tactggcatc cctccagccg cagacaccat acgttcacca
     5521 atctggatgt tcgccatctc catggtgaac agaagcacac catgcccctg ctcagtcacc
     5581 ttgtcgatga tgtccagcgc aagttcggtt ttccccatcg aaggacgggc ggcaatgaat
     5641 accaggtctc cgggctccat accgcctgtt tttgcgtcca gttcatcaat accggtcatc
     5701 aacgtcctgg atttctccag cccctgattc cggcattcaa cacgctcaac cacttccgga
     5761 agcacatcat caatatgtac cggctgaatg acgccctttc ctgtcgacag tgtgaccatc
     5821 atgttctgcg catccttcag ggcatcttca gctgcttcac aggtatgcgc atcacgtaat
     5881 ttctgcagcg cctcattcag tgttttttct gcatcgcgca gtgcggcatt gcgccgcaac
     5941 gctgcaacat agtgctccag tgaagacttc acccaggttt tacgcccggt atcagtaatc
     6001 accggggcaa gttccggcat ctcattacac aacagcacgg ggtcaatcac tcctgaaaca
     6061 cgggcctgtc tgcagatgcc tgtgtagata tcccgatacg ctcgtacaga aaaaatgtcc
     6121 gccggtagtg tgatcagaat atccatcact tcatgatctg ccccacgcag aaagaatgcg
     6181 ccaatgacag cgccttccag gtcatcatta cgccagactg gtgttgtcat gcagccacac
     6241 ctctgataca agaacggtag ctgggccagt tgaacgacaa ccagttgcgc cccccgtctg
     6301 tgatcctgtc ggcaatgcgg gggctgatga acgctggatt tgcccctata tttccagaca
     6361 cctgttatca cttaacccat tactggcttg ctgccgtaga tattcccgtg gcgagcgata
     6421 acccag
//
LOCUS       DAJTYZ010000190         6247 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000190 DAJTYZ010000000
VERSION     DAJTYZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.190"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..326
                     /locus_tag="OO596_003816"
     CDS             6..326
                     /locus_tag="OO596_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HCR6719558.1"
                     /translation="MTMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASY
                     RYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD
                     SQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="OO596_003817"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="OO596_003817"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6719559.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2213..2929
                     /locus_tag="OO596_003818"
     CDS             2213..2929
                     /locus_tag="OO596_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6719560.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3382..3457)
                     /locus_tag="OO596_003819"
     tRNA            complement(3382..3457)
                     /locus_tag="OO596_003819"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3422..3424),aa:Asn,seq:gtt)
     gene            complement(3558..5201)
                     /gene="yeeO"
                     /locus_tag="OO596_003820"
     CDS             complement(3558..5201)
                     /gene="yeeO"
                     /locus_tag="OO596_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR6719561.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5206..5281
                     /locus_tag="OO596_003821"
     tRNA            5206..5281
                     /locus_tag="OO596_003821"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5239..5241,aa:Asn,seq:gtt)
     gene            complement(5319..>6247)
                     /gene="cbl"
                     /locus_tag="OO596_003822"
     CDS             complement(5319..>6247)
                     /gene="cbl"
                     /locus_tag="OO596_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6719562.1"
                     /translation="IREAARQDYNLTEVANMLFTSQSGVSRHIRELEDELGIEIFVRR
                     GKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHTQARYSLPE
                     VIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVAFPWFRWHH
                     SLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLADIVLSAQD
                     SDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKRGQLQRNYV
                     WRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT         1773 a         1509 c         1351 g         1614 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccgccaacaa tataagtttt tataaaatta tggctttaga
     2101 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
     2161 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     2221 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     2281 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
     2341 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     2401 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
     2461 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
     2521 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     2581 ggcaggttct gtcagctata tgtttgacaa tgctggtgtg attgtattta aaggatcaga
     2641 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
     2701 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
     2761 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     2821 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     2881 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatcttttaa
     2941 agaaatctgt ctttacggca gatttcttta atctcatata attcttataa aaaatataag
     3001 attcaactcg tcatattgat tatacccccc cgttcccaga gaaataatat ttattaaaat
     3061 tccagttctt ctttttctga ttacagaagg caaagtggca attacgcata gtttcccgat
     3121 aaagacgcga tagcgacatc ccgcataagg catttttctc tttatctttg tacggtactt
     3181 catggaacag agtttttgac cttgcgaatc gtgatgtctg ttggggaggg gcaatttgct
     3241 cactgaagcg tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggtcact
     3301 tttttacaag gtaaccgggt aaaaataatt tttatttttt aactgttttg agactcatag
     3361 agatgtctca aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat
     3421 taacagtccg ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt
     3481 agcgatgccc acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat
     3541 aatctgcatt ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggttcg
     3601 gatcgagggt atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc
     3661 cgcacagccc agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg
     3721 atgcccagca cataaccgac gacaacccgg caaccccaca tgctcaacat agaaacccac
     3781 atggcgtaac gggcatcgcg agcaccttta aatccagcgg gtagcaccca tgaggcggac
     3841 caaataggca taaataaagc atttagccaa atcagaatca caacgacatg tttaacctgt
     3901 ggatcctggg tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg
     3961 gccgttaatc caagagtgga aagccagaac acatgccgca actgaatctc tgcttgcgct
     4021 atctgcccta cccccaacct tcggcctgta atgatcgtag aagcagagcc gagcgcactt
     4081 ccgggtaagt tgataagagc cgcaattgaa aacgcgataa aatttccggc aataacactg
     4141 gtccccatcc cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat
     4201 tcgacactcg cgggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt
     4261 ttaaaatagc tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac
     4321 aaaattgcaa ctgcgccaat ataacgagaa atggttaaac ccagccctgc cccgacaaat
     4381 cccagtcccg gccaggagaa aaggccgtaa atcaatatgc cgctaataat aatattaaga
     4441 atattcaggc taccgttaat caatagcggt attttcgtat tccctgcacc acgaagtgcc
     4501 ccgctaccaa taagagtgat ggcagctgct ggataactga gtaccgtcag ctccagataa
     4561 gtcaacgcca gtgctttaac ttctgtcgtg gcatcacccg cgacgaaatc aataatttgt
     4621 tcgccaaaat gatgaataag cgttgccaac agtacggcaa acaacgtcat gatcaccaat
     4681 gactgccgcg tcgccaccct cgctcgtcgt cgatcccgct taccgagact aaatgccaca
     4741 acgacagtag taccaagatc gatagcagca aaaaaagcca taatgaccat attgaagctg
     4801 tccgccaatc ccacgccggc catcgcatct tttcccagcc agctgaccag aaaagtgctc
     4861 agaaccccca tcaacaggac acaggcattc tccatgaaga taggaacagc aagcggggtt
     4921 atctcgcgcc agaacaacac tttgtagctc ttgcgtttag cgtgccagcg agtgccgtga
     4981 acaacctggc gtaaagcaga ggagatattc aaagccgacc ttaattgcag aaagtgaaac
     5041 cacatttcaa ataatgaggg agaatcagca aagctgcaaa gattttcgcc aacaaattgt
     5101 ctgcaaatgc aacaaactgt tgatagaaac ggcaaacagt tggggaattt aaaaatcggg
     5161 tttgacaaaa gatttttcgc cgttaagatg tgcctcaaca gcgattcctc tgtagttcag
     5221 tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc
     5281 aaatttctga aaagcccgct tttatagcgg gctttttgct atatctgata atcaatttcc
     5341 tcttcactgt tttccatcac ctgccgcttg atatcctcaa ctgacagtcc tgcattacaa
     5401 agttccagaa agcgccagac atagttacgc tgaagttgtc ctcgcttcag tcccaaccag
     5461 acagtattag catcgaaaag atgccgcgta tccagacgga ttaaattctc ttcctcttgt
     5521 tcgccgctgg attgttcggc aactaatccg atcccaagcc caagagcaac ataggtttta
     5581 atgacatcag aatcctgcgc acttaataca atatctgcca gcaaaccttt gcgggcaaat
     5641 gcgtcatcaa tacgtgagcg ccccgtaatc ccctgtcggt aagtgattaa cggccacttc
     5701 gctattgatt ccagcgtcaa tggtgtaatt tgcgttaagg gatgatcgag tggaacaagc
     5761 aaactatggt gccaacgaaa ccacgggaag gcgacgagct gcgggtcatt actcaaacgc
     5821 tcgctggcga taccaatatc agcttcgcca ttttgcaaca acgtcgcaat ttcctgtggc
     5881 gtcccctgga ttagctcgag ccgaacctcc gggaaaagtt cgcgaaaagc tttaatgacc
     5941 tctggcaagc tataacgtgc ctgagtatgc gtcgttgcaa tagtgagaac gccagacgta
     6001 tcgttggtaa acaggtctgc aagccgacga acattactgg cttcattcag aatacgttct
     6061 gcaatgacca gtaatgcttt gcccggttca gtcatgccca gcagtcgctt acctcgtcga
     6121 acaaatattt cgatgccaag ttcatcctcc agttcccgaa tatgacggct gacgccggac
     6181 tgtgaggtaa aaagcatatt cgcaacctct gtcaggttgt aatcctgacg tgcagcctcg
     6241 cggatta
//
LOCUS       DAJTYZ010000191         6226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000191 DAJTYZ010000000
VERSION     DAJTYZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.191"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..613)
                     /locus_tag="OO596_003823"
     CDS             complement(<1..613)
                     /locus_tag="OO596_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HCR6719563.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGD"
     gene            1309..3594
                     /gene="nrdA"
                     /locus_tag="OO596_003824"
     CDS             1309..3594
                     /gene="nrdA"
                     /locus_tag="OO596_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6719564.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3828..4958
                     /gene="nrdB"
                     /locus_tag="OO596_003825"
     CDS             3828..4958
                     /gene="nrdB"
                     /locus_tag="OO596_003825"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6719565.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4958..5212
                     /gene="yfaE"
                     /locus_tag="OO596_003826"
     CDS             4958..5212
                     /gene="yfaE"
                     /locus_tag="OO596_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6719566.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5289..5915)
                     /gene="inaA"
                     /locus_tag="OO596_003827"
     CDS             complement(5289..5915)
                     /gene="inaA"
                     /locus_tag="OO596_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6719567.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            6094..6226
                     /locus_tag="OO596_003828"
                     /pseudo
     CDS             6094..6226
                     /locus_tag="OO596_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1608 a         1606 c         1462 g         1550 t
ORIGIN      
        1 catcgccatc actgtaactt gttgatgtat atgcctggtt gttatcaaaa ataacatcac
       61 ttaaatagac gtcattatta atggtataaa ttgcgccacc tttgccatca ttagcgatat
      121 tattgcgaaa catggcgtta gtgacactta aatcgacggc accagtatcg ttagtaccag
      181 aagaatagat tgcgccacca tagccgcctg cgacgttacc ggaaaaaatg acatcagtaa
      241 gattcagcgt tgagttttct ttagcaaata tggccccgcc gttataatat tctcctgtaa
      301 cggtgttatt ggcaaacaga gtcattccag tttcgttttc tggtgataat gtaaattccg
      361 ctccttgttg caaaaatacg gccccaccgc tggcattatt ggtcatatcc gaaaaaacca
      421 gccattgccc atcggcgata ctccagtcct gcgtaatgcc tgaaaggctt tgcctgctgg
      481 cgtgacaact cgctttgata tcatatccct ggcataaatc aatggccgcc gcgacaccgt
      541 tagcagagaa aagagatgca atcattgacg ggagtagaga taaatactcc ttgcgtagaa
      601 agataatccg catattaatc tgaccatcat tttatataag acggcatatt taaacgcatt
      661 atacaatatg ggttatcttt cttttgtaaa ttgtccaacc cgatgcaatg gctattttgt
      721 tattatttga tgaataatat cagtgcgtca taattcaagt taataacctt aagggatacc
      781 agttatattt aaactaaatt atagtaatga ataattttct tataatataa ggtaaattaa
      841 caaaatggct tcgcatttaa caataaccga atagaaaaca accatttcga catcaacaat
      901 cgcttacatt cgcttatata ttgaccacaa ctgatacatc agattatgtg atgactcgtg
      961 cttagatcaa tttttgcaat cattaacaaa aagattaata aaccatctat atcaatttat
     1021 ccaacctatt ctgccgttca agaaatcgcc gaacagttat ttttaacaaa tttttctctt
     1081 cccattgact ttcccggaca ccttgtctga cctaaggtgc gcgaaagcca ctttttcctt
     1141 cctgagttat ccacaaagtt atgcacttgc aagagggtca ttttcacact atcttgcagt
     1201 gaatcccaaa cataccccct atatatagtg ttctaagcag cttcccttac tacaggtagt
     1261 ctgcatgaaa ctattgcgga aagaattcca aaaacaggta cgacatacat gaatcagaat
     1321 ctgctggtga caaagcgcga cggtagcaca gagcgcatca atctcgacaa aatccatcgc
     1381 gttctggatt gggcggcaga aggactgcat aacgtttcga tttcccaggt cgagctgcgc
     1441 tcccacattc agttttatga cggtatcaag acctctgaca tccacgaaac cattatcaag
     1501 gctgccgcag acctgatctc ccgtgatgcg ccggattatc agtatctcgc cgcgcgcctg
     1561 gcgatcttcc acctgcgtaa aaaagcctac ggccagtttg agccgcctgc gctgtacgac
     1621 cacgtggtga aaatggtcga gatgggcaaa tacgataatc atctgctgga agactacacg
     1681 gaagaagagt tcaagcagat ggacacgttt atcgatcacg accgtgatat gaccttctct
     1741 tatgctgccg ttaagcagct ggaaggcaaa tatctggtac agaaccgcgt gaccggcgaa
     1801 atctatgaga gcgcccagtt cctttatatt ctggttgccg cgtgcctgtt ctcgaactac
     1861 ccgcgtgaaa cgcgcctgca atatgtgaag cgtttttacg acgcggtttc cacatttaaa
     1921 atttcgctgc cgacgccaat catgtccggc gtgcgtaccc cgactcgtca gttcagctcc
     1981 tgcgtactga tcgagtgcgg tgacagcctg gattccatca acgccacctc cagcgcgatt
     2041 gttaaatacg tttcccagcg tgccgggatc ggcatcaacg ccgggcgtat tcgtgcgctg
     2101 ggtagcccga ttcgcggtgg tgaagcgttc cataccggct gcattccgtt ctacaaacat
     2161 ttccagacag cggtgaaatc ctgctctcag ggcggtgtgc gcggcggtgc ggcaacgctg
     2221 ttctacccga tgtggcatct ggaagtggaa agcctgctgg tgttgaaaaa caaccgtggt
     2281 gtggaaggca accgcgtgcg tcatatggac tacggggtac aaatcaacaa actgatgtat
     2341 acccgtctgc tgaaaggtga agatatcacc ctgttcagcc cgtccgacgt accggggctg
     2401 tacgacgcgt tcttcgccga tcaggaagag tttgaacgtc tgtataccaa atatgagaaa
     2461 gacgacagca tccgcaagca gcgtgtgaaa gccgttgagc tgttctcgct gatgatgcag
     2521 gaacgtgcgt ctaccggtcg tatctatatt cagaacgttg accactgcaa tacccatagc
     2581 ccgtttgatc cggccatcgc gccagtgcgt cagtctaacc tgtgcctgga gatagccctg
     2641 ccgaccaaac cgctgaacga cgtcaacgac gagaacggtg aaatcgcgct gtgtacgctg
     2701 tctgctttca acctgggcgc aattaataac ctggatgaac tggaagagct ggcaattctg
     2761 gcggttcgtg cacttgacgc gctgctggat tatcaggatt acccgatccc ggccgccaaa
     2821 cgtggagcga tgggtcgtcg tacgctgggt attggtgtga tcaacttcgc ttactacctg
     2881 gcgaagcacg gtaaacgcta ctccgacggc agcgccaaca acctgacgca taaaaccttc
     2941 gaagccattc agtattacct gctgaaagcc tctaatgagc tggcgaaaga gcaaggcgcg
     3001 tgcccgtggt ttaacgaaac cacttacgcg aaagggatcc tgccgatcga tacctataag
     3061 aaagatctgg ataccatcgc taatgagccg ctgcattacg actgggaagc attgcgtgaa
     3121 tcaatcaaaa cgcacggcct gcgtaactct acgctttctg ccctgatgcc gtccgagacc
     3181 tcttcgcaga tctctaacgc cactaacggt attgaaccgc cgcgcggtta cgtcagcatc
     3241 aaagcgtcga aagacggtat tttgcgccag gtggtgccgg actacgagca cctgcacgac
     3301 gcctatgagc tgctgtggga aatgccgggt aacgatggct acctgcaact ggtaggtatc
     3361 atgcagaaat ttatcgatca gtcgatctct gctaacacca actacgatcc gtcacgtttc
     3421 ccgtcaggaa aagtgccgat gcagcagttg ctgaaagacc tgctcaccgc ctacaaattc
     3481 ggggtcaaaa cgctgtatta tcagaacacc cgtgacggcg ctgaagacgc gcaggacgat
     3541 ctggtaccgt caatccagga cgatggctgc gaaagcggcg catgtaagat ctgatattga
     3601 gatgccggat gcggcgtaaa cgccttatcc ggcctacggc tcggtttgta ggcctgataa
     3661 gacgcgccag cgtcgcatca ggctccgggt gccggatgca gcgtgaacgc cttatccggc
     3721 ctacggctcg gatttgtagg cctgataaga cgcgccagcg tcgcatcagg cacaggatgc
     3781 ggcgtaaaat gccttatccg gcattaaact cccaacagga cacactcatg gcatatacca
     3841 ccttttcaca gacgaaaaat gatcagctca aagaaccgat gttctttggt cagccggtca
     3901 acgtggctcg ctacgatcag caaaaatatg acatcttcga aaagctgatc gaaaagcagc
     3961 tctctttctt ctggcgtccg gaagaagttg acgtctcccg cgaccgtata gattaccagg
     4021 cgctgccgga gcacgaaaaa cacatcttta tcagcaacct gaaatatcag acgctgctgg
     4081 attccattca gggtcgtagc ccgaacgtgg cgctattgcc gcttatttct attccggaac
     4141 tggaaacctg ggtcgaaacc tgggcgttct cagaaacgat tcattcccgt tcctatactc
     4201 atatcattcg taatatcgtt aacgatccgt ctgttgtgtt tgacgatatc gtcaccaacg
     4261 agcagatcca gaaacgtgcg gaagggatct ccagctatta cgatgaactg atcgaaatga
     4321 ccagctactg gcaccttctg ggcgaaggca cccacaccgt taacggtaaa accgtaaccg
     4381 ttagcctgcg cgagctgaag aaaaaactgt atctctgcct gatgagcgtt aacgcgctgg
     4441 aagcgatccg cttctacgtc agctttgctt gttcctttgc ctttgcagaa cgcgaactga
     4501 tggaaggtaa cgccaagatt attcgcctga ttgcccgcga tgaagccctg cacctgaccg
     4561 gtactcagca tatgctgaat ctgctgcgca gcggcgcgga tgatccggag atggcggaaa
     4621 ttgccgaaga atgtaagcag gagtgctatg acctgtttgt ccaggcggca cagcaagaga
     4681 aagactgggc ggattatctg ttccgtgacg gttcgatgat tggtctgaat aaagatattc
     4741 tctgccagta cgttgaatac atcaccaata tccgcatgca ggcagtcggt ttggatctgc
     4801 cgttccagac gcgctccaac ccgatcccgt ggatcaacac ctggctggtg tctgataacg
     4861 tgcaggttgc tccgcaggaa gtggaagtca gttcttatct ggtcgggcag attgacgcgg
     4921 aagtggacac cgacgatttg agtaacttcc agctctgatg gcccgcgtta ccctgcgcat
     4981 cactggcaca caactgctgt gccaggatga acacccttcc cttctggcgg cgctggaatc
     5041 ccacaatgtt gcggttgagt accagtgccg cgaaggttac tgcggctcct gtcgcacccg
     5101 actggtcgca ggtcaggtgg actggattgc cgaaccgtta gcctttattc agccggggga
     5161 aattttgccc tgttgttgcc gggcaaaagg cgatattgaa atcgagatgt gaattggttg
     5221 tagtgccaga tacaacgctt atacgtctta tctggcctac aacgattaca tggcgtagta
     5281 atacgctttc acctgctccc agtccgcttt ggggattggc tccagatatt ttccagctgg
     5341 cggaagtcat gattaatcgc tttattgcga cgcaagcggc gacgactttt ctccaggtca
     5401 agaaaaccag cttctgcttt accttctgtt ttcacataga tatggcgaac ataacaacag
     5461 ccatgctgac gattaacgct atgcattttc ttaaacgcca gcgccactgc tttcaacatg
     5521 gcttgccgta cttcgtcaga atagggcgat actgcatgcc gggcatacca gtcagcaatg
     5581 ctgatgaacc ccgccatatc ttcagtcacc agcaacgcgc gccattcacc ctcaattttc
     5641 accgcttcac caaaaacgat cttcggcacg atgacaccgg cccgttccag ttctttaatt
     5701 accgcaacct cacggacaat cgttggtcgg ccgaacggat aacgtacgga atgaaacaga
     5761 tgatgcgtca tgcgctttac atacagcttt ttgccgttgc gctcgacgca ttgcaccccg
     5821 ctcataccat tacggcgata gttaggctct tcaacccagt cgccctctgt tgcccaccag
     5881 tgattaaatt cgtcgtactt tgctgaaact gccataccct atcgcctgtc atttttatta
     5941 acgacaatga ctataggtgg ttacctgagg aaaatcttaa tgaaacgtgt cgtattaatg
     6001 aatttctttt gccgctacta gtgtgacacc gcttttgccc ttcatttgca gcgcatagat
     6061 tccaccagcc acgaccacta atagtgcgcc cagcagataa aatgtgaatt taatcaggtg
     6121 gatacagcga tgaattttat tcgacaggga ttaggcatcg ccttattacc agagttaacg
     6181 ctgaaaacca ttgcaggtga attgtgttcc gttcctcacg aaccaa
//
LOCUS       DAJTYZ010000192         6166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000192 DAJTYZ010000000
VERSION     DAJTYZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.192"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..572)
                     /locus_tag="OO596_003829"
     CDS             complement(<1..572)
                     /locus_tag="OO596_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6719568.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDP"
     gene            complement(934..3717)
                     /locus_tag="OO596_003830"
     CDS             complement(934..3717)
                     /locus_tag="OO596_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6719569.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3774..6146)
                     /locus_tag="OO596_003831"
     CDS             complement(3774..6146)
                     /locus_tag="OO596_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6719570.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1556 a         1416 c         1398 g         1796 t
ORIGIN      
        1 gggtccataa acaactgacc ggggcgcata gggatctcac gcagctccac atcccagtag
       61 cgggcgaatt tatgccagca gatttgtacc ggaccgcaca ccaggtttgg tttatccgtt
      121 ggtttgcctg cagcttccat acgcttgcgc caacgccatt tcatcgccat cccgccgagc
      181 atacaggcct cggaagaacc aatggtgttg gtgccaacgg cctgaccatt tttcggcgca
      241 ggcgcatgcc acagatcggc aaccatattt acgcaacgca ggtcgatggc tgcggattgc
      301 ggatattctt ctttgtcgat ccagtttttg ttaatggata aatccatcaa tttgtggaca
      361 ttttcgtcgt cccaggtctg gcagaaagtg gccaggttct gacgagcgtt gccatcaaga
      421 tataattcgt cattgataat ctggaatgcg acatcgtcgc gcatttcgtg cagcggaaaa
      481 cgttttgatt ctgcgatagt ggaaatagac ttcgcaccaa aacgtgaatc gagtagttcc
      541 gaccttaaat ccgttacttg cttcttatcc attttaaact ccttaaaatg attggatagc
      601 attaaaaaag taggatttat cgataaagta agcaagtgca aaggactcgt gtttaaataa
      661 caaaatccta atgttattta tcgtgagata ttgcgagaat aatatttttt cattgaaaaa
      721 caatataata tgaaattctt gggtggtggt aaggtgtttt atgctgttat ttttatgcgc
      781 attctgtgcc tcctgaattt tcacgtaaaa atcagacctt aaaatatcac tattagtacc
      841 tggttattat tttgaacgca tttataaaat tattacataa aaatggcgaa tattgctaaa
      901 atccccgcca acgatgtgtt gacggggctg ttattatttt ggcaataata ctccagtata
      961 agtatttacc ggatgagaaa gatattgttt aacggcagtg ttaacattct ctaccgtcat
     1021 ttgtttcaac aattgctcct gctcagtcca tgctgcagga tcgtcatatt gaataagact
     1081 atttacaata gtgttcgcta attgttgaac gctacgctgt tggatatcga ggctgcgctg
     1141 aacgttttgc tggtattcat tcagttcttg ctcactgatc cctttagcca gacgcttaac
     1201 catcacttca ttcgctaacg ttaacagttc atcatgtcgt tctggttgac aagtaaaagc
     1261 cagcaaatga ctgatatctt tggcctgagg atcaaccgag aggcgagaag aaacgctgta
     1321 tgctccagat gcctgttcac gaatattaac gcgtagatct tttgccagtg cgacgttaaa
     1381 agcatcgagc gccatacgcg tcgccagatt aacaggtgtc cgggaatcat aacgcttcca
     1441 ctgtgaaacc tgtgccacag gttcattttg ttcttttaca gtaaccgatg cgttgtccgt
     1501 cgcgcgagtt aatggtttac ctgcggctaa tggcgaatca gagtgtttga ttgatcctaa
     1561 gtaacgcgta attaacgcca cgagtttgtc ttctgagaca ttaccgacaa tgacaaacgt
     1621 gatatccgct ggagatgaaa acaactggcg atcggcagcc agcgcatctg cggcagtaaa
     1681 ctgtacaatc tgattttctt gcagtaattt cgtgcggtca tcagcatagc gcgtctcata
     1741 catctgctgg gcgaatttct ccgctggacg ctggtcgagc gttttcaacg cctgaatttg
     1801 agcattttgt agcgatgccc aaatattgtc gttaatcgtg ctgtgggtga ttcgctggtt
     1861 aatcaactga aaaccaggtt cagggttatt agtccgcgcg ctaacggaga gcaacgtatt
     1921 catgccactg actttactgc tcatggttac cgaattttcc gcactccagc gtttcaggct
     1981 ggaagaggag agctcaccaa cgccgcttcc gcttactgct ttatttgcca gcgcgataag
     2041 tgatttttgc tgcgcaggga aacttaaatc gcctttatta gatacggcag taatttgtag
     2101 cttttgctct tcacccgcgg attttgccag aataaccctg gcaccattgg aaagtgttaa
     2161 tgatgtcaga ttctcagcca gcgtttcttt gctgctaatt tccgcctgtg ggtcagcgtc
     2221 tactgttaac gataaatttc tgcctgggaa gatgtaagcc gccagctttt tgttggcgta
     2281 ctctttttcc agcgccagga tagccgcaag agacaatgct tttttggcgg caagctcatt
     2341 gtttaccatt tgctcccaaa atgcgtcctg gttctttctt aactgctgcc atttttccgc
     2401 cagactttgc acggtaattt gctgccacag acgtttcgaa agttgatatg tctcttccgg
     2461 cgacaagaac ggcgtattat ttaatgagct ggatgccagg cgactggtca gcatacgtaa
     2521 atcacgttca gcttgctgat caaccgcatt tttcagccag gtgaggcggg tagatttgac
     2581 atcatcgagt tcttcagcag aaaaaccatg ctgatcaatg gttgccaact ctgccattaa
     2641 tgcatttgca gcatcctgca tattatcgtc tcgtgcattt acacggaaaa acagcgactg
     2701 ataatcgggt gcaattttaa cgctgcgcgc agtgccgcca gaaatagtct tcaactcgcc
     2761 cgactgtatg cgttcctgca gacgttgatt gaacagctga actaacatgc tccattcagc
     2821 ttgttcgaca aagctttgct catcgttcac ttgtaccatt ggcaggcgat aatagagtgc
     2881 gatgccgttc actcggtttt ctttatcatt aatgatatta aagcgcaggt ggttttcggc
     2941 ttttgtcggc cagacgcgat tttcagctgc tttgttagcc ggaagcttac ttaaattatc
     3001 ctttatcagc gccagcgctt ctttactgtc gatatcgccg accacgataa aggtcatatt
     3061 atttggttga taccagcgtt gataaaattg gcgcaattgt gccggtgtga ccgtggcgac
     3121 agtatccatc aggccgatag gttcacggtc taaattacgg gtatttgcca gcaggaaagg
     3181 gcggcgcgcc tgagaggtgc gccatttcgc atcctgatgg gcacgccatt cctcagtaat
     3241 tacgccgcgt tcagcgtcta cttcgagtat ttcaaaggtt gcggcattac tccattcact
     3301 gaagattgcc atcacttgtt gcagattttg tttctgcgta gtcggcagac tcacctgata
     3361 cagcgtttcg tcatagctgg tataggcatt aacatcgcga ccgaaacgca ggcccattga
     3421 ctcaaatgtt tcgatgactt tattacccgg ccatgttttt gtgccgttaa acatcatatg
     3481 ttctacaaaa tgagccacgc cgcgctcatt gtcttcttcc tgcaatgaac cggtatgaat
     3541 ttgcagccat aaatttacct gatcctttgg ttgagcatgc ggataaatca tatatcgcaa
     3601 gccattgtcc agttgcccgg taattaactt ttcatcctgc ggtaaggcgg cggcaatcag
     3661 ccgcccgggg agcaacagag ttgccactaa cgtcagtaag aaacagaggt ttctcataat
     3721 tatctccatg cgaaaaccgg gcgaatttac ccggttaagt aaaatccgaa ctattaaaat
     3781 ttcatgctga catcaagcca gaaagtacgg ccactggcgt aggtcgccac accggtgttc
     3841 gttgtatcaa cagcggtttt gctatccagt acattgagaa tatcggcact gatcgtcagg
     3901 ttttgttgtt tcagaaattg tggcgtccag gacaagcggg tgtcccaggt caggctgcta
     3961 tccagttttt cgtcaacata ctgcttgtaa tctgaatatt cgctgatgta ttgcgcattt
     4021 gtcttaccaa ggataatgcg agctttacgc gcttcttgcc aggccagcgt attcgcccac
     4081 actaacccgc tcggttgatg cgtgaaatcc atgtttaagg agatctttaa tgggttatta
     4141 aaatctgcca ctggaacact atcgtaagag accagattac cgttataaac cacctggtta
     4201 tcaccggtat tgctctcctc ataaccattg tttaacgaca agttgccctt gctcttgata
     4261 tagctaaaga caatttgtgg gttaatatct acttggcgga tatgcagggg ttcggccagt
     4321 tcaaaactga ggctgaacga atgcgttttg gttttgccat cgttgttata ttcagtaatg
     4381 gtggttttag tcgcgctgtc ggtacgactg cttttgctga tttgatcatg cgcttcacgg
     4441 taaacatagt ttgcacgcgc aataacgttc ttaccgattt tctgctgcaa tcccattgcc
     4501 agttcatcgt tataaggcgt tttcaaatcc tgataacgcg tcagagtttt attacctgat
     4561 accgattccg tccagctatt gcggatatca cgtaatccca tatcaagaat attcccgcca
     4621 tagtaacggt tataaccggc ggtaatcatt gaggtttgat cagcaaaaat atcccattcc
     4681 gtcataaagc gcggggagat attgtggttt gacagatagt tgtcatagtc ataacgcaca
     4741 ccgggcatta acgacacatt ccgccagcta atgtgctccg ccatatacag cgtgtagttg
     4801 tcaattccca ggctgccttt acctttatgg taaatggtat ggttagtttt ctttccggca
     4861 gcattaatca cataggattc agactggtta tggcgttcag tccatgcatc ggaatagatg
     4921 tattccgcgc cgaagtaggg ttgatgcgaa acatcaccca cggcgaattt ttgccagtcc
     4981 aggcgtgttt tgaaggtgta attatctaca gcctgggaaa tgtgtcctaa tccgccacag
     5041 gtacaacgcc ctgtaatatc accatatgta catgaaagtt cggtgtacca gatgtcatga
     5101 tcgtgacggg tataatcact aatatgatcc caaccaacgg tggtacgtag tttggcccat
     5161 gcgagttgcg tatccatatc ccatgccaga ccataggatt tattacccat ttcgcgatca
     5221 gactgcggga aggtgctggt attataatca cggctggagc cggtatattt taaggttaaa
     5281 tcgtgggtaa agcggtcgct ggcaaaccag gtaaatttgc tcaatgcagt atcgataacg
     5341 tttttatact gtgcccgacc ggtgacaatg ccgtcattcg aaacataatc cgcgcgggtg
     5401 atatcagact ggcggcgcga taaaccggcg gtaacgccga agttatcagc gagttcctga
     5461 ttaaacgaca aggtataaaa gttcttttta aaatctgggg agtaataagt acttcctgaa
     5521 gagccttgat taaatgcgct cttgttattc tcatcgatat gcgatgttaa ccagtccgaa
     5581 cgcgtagtgc gataacccaa tttcaccttg ctatcatcag cgttgaagcg tttgatcttt
     5641 gcatcaatta ccccgccatt gaagcgacca aattcaaccg gcacaaaact gtcataaagc
     5701 gtcacattgt ccagtaagct gacatcaaga taatacccct gtgacatccc gctaatattg
     5761 gttgcactac tggcatcgga ctcattcgct gggttcaggt tattagttgc actaataccg
     5821 tcaatcaaat aggcattctg gtagggcgac gcaccgtgaa tagagatttt ctcagggcga
     5881 atatctccct ggttcaacga ggtactttgc gttgaatcca tgcgtaccgc agggttggtt
     5941 ctcagcagat cgctgatatt gccattcccg gtcggtaaac gctcgatgct ttcactggtg
     6001 taatgcgtgt tgccgtttac gggtacaggg acaggagcat agaccgtcat ctcttcaaaa
     6061 aaatgcatgt acatgttttt gtcatcgacg gcctgcgcca catcagcgcc acataaaatg
     6121 acgccaggaa taagaactcg cttcatttat ttatatctcc gtacag
//
LOCUS       DAJTYZ010000193         6132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000193 DAJTYZ010000000
VERSION     DAJTYZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.193"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            34..864
                     /locus_tag="OO596_003832"
     CDS             34..864
                     /locus_tag="OO596_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HCR6719571.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1005..1778)
                     /gene="uxuR"
                     /locus_tag="OO596_003833"
     CDS             complement(1005..1778)
                     /gene="uxuR"
                     /locus_tag="OO596_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6719572.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1993..3453)
                     /locus_tag="OO596_003834"
     CDS             complement(1993..3453)
                     /locus_tag="OO596_003834"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6719573.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3534..4718)
                     /gene="uxuA"
                     /locus_tag="OO596_003835"
     CDS             complement(3534..4718)
                     /gene="uxuA"
                     /locus_tag="OO596_003835"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6719574.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5058..>6132
                     /gene="gntP"
                     /locus_tag="OO596_003836"
     CDS             5058..>6132
                     /gene="gntP"
                     /locus_tag="OO596_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6719575.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGV"
BASE COUNT         1446 a         1595 c         1567 g         1524 t
ORIGIN      
        1 acatcagtga aatactcata aaaggtaact attatgtcta tgccattaag caatgccctt
       61 cagagtcaaa ttattacaga taatcacttc cttcatcacc caatagtcga cagtgagttt
      121 acacataaat taaaatatgc acgcatggat ttggaaaaca tctatcttcc gcctcttact
      181 cgtggcaata accataatta cgatggaaaa agcgctgtcg aaattagaaa acttgatatt
      241 agtaaagagt cctggccatt taattatgtg acaggagcat gcagagaatc cgatggtatt
      301 acaacaacag gacgtatgct ctatcggaac ttgaaaatca cctcagcttt agacgaaata
      361 tatggtggca tatgtaaaaa ggcgcatgct gccacagagt tagcagaagg tttacggcta
      421 aatttattta tgaagagccc tttcgatcct gttgaggact atacagttca tgaaataaca
      481 ttaggccctg gttgcaatgt tcctggttat gccggaacca cgataggata tatttcaacc
      541 cttccagctt ctcaggcaaa aagatggacc aatgagcagc caaggattga tatctatatt
      601 gatcagataa tgacggtaac aggggtagca aattcttccg gattcgccct tgccgcatta
      661 cttaatgcta atattgagtt gggtaatgac ccaataattg gtatagaagc ctatcccggt
      721 accgcggaga tccatgcaaa aatgggatat aaagttatcc cgggtgatga aaatgcacca
      781 ctcaaacgta tgactctgca accgtcatct cttccagaac tgtttgaatt aaaaaatggt
      841 gagtggaatt atattggtaa ataactacca ggaagaatga gtactaacat tcaggttata
      901 cagatagcta aacaataccc tgcaatttac aaagtaatac tcattgcgac ttcttaattt
      961 aagaaagcgc aaggaacatt tacccttgcg cttattataa taagtcaggc gtcaacttta
     1021 tccagcggcc atgaatcaaa cagatagcca tcaaaataaa tatcgtcaac gttcgagaat
     1081 tccagcagac gttgcttaac gttttccaga tgccgccaca ttgccagctt cgccgctcgg
     1141 gcatcttttt tgattaacgc ggcgaggatc tgtttgtgat cgcccaacca ctctttgcga
     1201 tacaggctgt catccagatg gctgtgcaac tggatccaca ttggattgtt ttcccgccac
     1261 tgccaggact gacggaacag ctccaccagc atgctgttat gcgttgcttc ggcaatggcg
     1321 agatggaact gcatatcacc actttcgctg ctgcccggcg cgctggaagc cagctcacgc
     1381 tcttccagtt gcaatgcctg acgcattttg acgatatctt cgcgggtagc ctgcaaagcg
     1441 gcaaattccg cgatgttact ttccagtaac tgacgcgcct gtaacagctc aaaaggaccg
     1501 gcatcattgc agacgttagc atccggactg tctgtgttgt ggctgcctga gctatcaaga
     1561 acatagatac cggcaccccg gcgcacttcc accagccctt tgatctccag catgatcagc
     1621 gcttcacgta ccaccgtcct cgtgacatca agcatttctg caatttcacg ctccggcggc
     1681 agccgttcgc caggattgta tggcgtcttt atgatcagat cgcggatcat cgccccgact
     1741 tcctggtaag gtctttgcgc agaggtggca gatttcatgg tgttgtgtcc gttaggtgcg
     1801 gttaatcaaa tttttgccgg atgcggcgtg aacgccttat ccggcctaca acgggcaatt
     1861 ttacggcacc cgttaatctc aaactatacc attagcactg gaagttggca cgtacaggtg
     1921 ggcaaaaaaa cgaaaactgg tcaaccaaat atcagtttga ttgaccagta agtctgtagg
     1981 gtaatcagtt agttacagcg cagccacaca ctcgcgcgca ccgcgttcgc acagctgctg
     2041 atatgccgcc gtcaccgcgc caacaaagtc ggcattctgc ggcagatcat cggcaaaaat
     2101 gccgctcagg cccagcagcg ctttcacgcg gtctgcgccc tgatactgcg cgttaatctt
     2161 ctggaactcg gccagcatcg ggtcaaccac gtcaatggca ttaccctgtt catccacgcc
     2221 ctgggtgtag cgcatccagc cagccacgcc cagtgccagg tgacgccagc tgccgccgtt
     2281 ttgcaggtgc agacgcaccg gatccagaag acgctgcggt aacttctggc tgccgtccat
     2341 tgcaatctgc caggtacggt gacgcagaga cgggttgctg aaacgctcga tcagcagcgt
     2401 cgcataggcg ttcaggtctg taccttccgg catcgacagc gttggcgctt gttcctgcat
     2461 catcagggca aaggccgctt tgcgataatc cggattagtc atggtgtcgg caatggtttc
     2521 atagccgccg aggtaaccga ggtacgccag gaaagagtgg ctgccgttca gcatacgcag
     2581 tttcatcatt tcgaacggca caacgtctgc aacgaactgt gcgcccactt tatcccagtc
     2641 cggacgacca ttaacgaagt tatcttcaat cacccactga cggaacggtt cgcaggcaat
     2701 ggcgcacggg tcgtaaacac ccagctggtc agcaatttcc tgtaaggttt ctggcgtcgc
     2761 cgccggaacg atgcggtcaa ccatggtgca cgggaaggtg acgttctctt caatccatgc
     2821 cgccagctgc ggatcacgtg cctgagccag ccccagaacc gcgaccttcg ccacatgacc
     2881 gttttcacgc acgttgtcgc aggacatcac cgtaaacgct ttcagccctt tttcacgacg
     2941 cagacgcaag gcttcgacga tgtaaccaat cgcggacttc ggcgcagtcg ggttttccag
     3001 atcgtgcttg atcagcgggt tattgagatc cagctgaccg cttgccgcat cggcgcaata
     3061 acctttctct gtgaccgtca gggagacaat cgccgtctgc ggacgcgcca tcgcgttgag
     3121 aataccttca caaccgtcga tttccggatg cagggcttct ttcatcgaac cgataatttt
     3181 cagctcggtg ctttctgcgc ctttttccgc cacggtgtac agcagttgct gttttttcag
     3241 gttttcaatc agcacacggt cgttgcctgg catcaggtta acttcgcaga taccccagtc
     3301 gctgtcggta ctttccagca gatgatgggt gtacagcgcc tggtgcgcgc ggtgaaacgc
     3361 cccgcaaccg agatgcacaa tgcgtgattc cagacgagaa tgatcccatg acgggcgggc
     3421 aaccggcaga ttgctgtcaa caatagtagt catcgtaaac tccagaatcc gtcttcagag
     3481 agtgacggca aggaagaagg aaccggcacg gcgcgcagcc atgccggtgg gtatcaacgg
     3541 ctaaagaaag cgcgctggat cgccagttcg acaccgcgaa cttcagccag gcctttcaga
     3601 cgaccaattg cggagtaacc tgggttggtt ttcttcttca ggtcgtccag catctgatga
     3661 ccgtggtccg gacgcatcgg gatcaggtct tctttgcctt ccgctttacg acggtgttct
     3721 tcttcaacaa tcgctttcac cacttcgtac atatcaacgt caccgttcag gtgcgccgct
     3781 tcgtggaagg ttttcgggtt atcttcacgc atggtggagc gcagatgggt gaagtaaata
     3841 cgcggaccga actgcttgat catatcaacc agatcgttgt cagcacgcac gccgtaggaa
     3901 cctgtgcaca tggtgaaacc gttcgccatg ctgtttacgg tatcaaccat ccactgcata
     3961 tcttcaatgg tggaaacaat gcgcggcagg ccgaggatcg ggcgcggcgg atcgtccggg
     4021 tgaacagcca tacggacgcc aacttcttcg gcaaccggaa taatcgcttt caggaagacg
     4081 gcaaagtttt cgcgcagttt ggctttgtcg atatctttat acagctccag gtgcttacgg
     4141 aactggtcga gggtataccc ttcttccgcg cccggcagac cggcaatgat gttacgggtc
     4201 agacgcgctt tgtcttcgtc gctcatagtg gcgaagcgtt cagcggcctg agcaatttct
     4261 tcttcggtgt aatccgcttc cgcgcctgga cgcttcagga tatgcatttc gaatgcagcg
     4321 aattcgatct ggtcgaagcg cagagctttg gagccgtctg gcagcacgta ttcgaggtca
     4381 gtacgggtcc agtcgagcac cggcatgaag ttgtagcaca cggtgcgaat gccgcactgc
     4441 accaggttgc gcagagtctg ctgatagtta gcgatccact gttcatagtt gccagtgtgg
     4501 gttttgatat cttcgtgaat tggcacgctt tctacgacag accataccag gcctgcgtct
     4561 tcaacgatcg ccttgcgttt gaggatctct tctacggacc atacttcgcc gttcgggata
     4621 tggtgcagcg cggtaaccac gccagttgcg cccgcctgac ggacatcagc tagagaaacc
     4681 ggatcgtttg ggccgtacca gcgccaggtc tgttccatga tttttcctct ttcacatagt
     4741 gggttccaca gcaaagagac gtggagcccc ggagttgttt aggtatccgg atgaacaacc
     4801 agaccgcagc cgtggcgttg catcggatga gatggcgtat aagttctacc ttttggtaag
     4861 acatgtcaac ccgaaatctg aaattggtta accacatcgc aagaatttca ctctcacggt
     4921 tgttgctgca aattgcagca aagggtaatc gatggttatt gatcagagga tgtgacattc
     4981 atcgcaacaa tggttgacca atttacataa catgtcgccc aaaataacaa cggttcaacc
     5041 aggtaaggta aaacgttatg catgtgctta acattctctg ggtggtattc ggcattggcc
     5101 tgatgctggt actgaatttg aagttcaaaa tcaattcaat ggtggctttg ctagtggcgg
     5161 cgctgtccgt cgggatgctg gcgggcatgg atttgatgtc gctgctgcac acaatgaaag
     5221 cgggcttcgg caacacgctg ggggaactgg ctatcatcgt ggtgttcggt gcggtcatcg
     5281 gtaaattgat ggtcgactcc ggcgcggcgc accagatagc gcatacgctg ctggcgcgtc
     5341 tcggtctgcg ctatgtacag ctgtcggtaa ttatcatcgg cctgattttc ggtctggcga
     5401 tgttctatga agtggccttt atcatgttag cgccgctggt tattgtcatt gccgccgaag
     5461 ctaaaatccc gtttctgaaa ctggcgatcc cggcagtagc agctgccact accgcacatt
     5521 cactgttccc accgcagccg ggtccggtgg cgctggtgaa tgcttatggc gcggatatgg
     5581 ggatggttta tatctatggc gtactggtga cgatcccaag tgtcatctgc gcaggtctga
     5641 tcctgccgaa gttcctcggc aatcttgagc gcccaacgcc atcattcctg aaagcagatc
     5701 aaccggtaga tatgaataat ctgccctctt tcggcgtttc gattctggtg ccgctgatcc
     5761 cggcgatcat tatgatctct accaccatcg ccaatatctg gctggtaaaa gatacccctg
     5821 cctgggaagt ggttaacttt atcggttcct cgccgattgc aatgtttatt gcgatggtgg
     5881 ttgcattcgt actcttcggc accgcgcgcg ggcacgatat gcagtgggtg atgaacgctt
     5941 ttgaaagcgc ggtgaagagc attgcaatgg tgattctgat catcggtgcg ggtggcgtgc
     6001 tgaagcagac catcatcgac accggcattg gtgacaccat cggcatgtta atgtcccacg
     6061 gcaatatctc gccctacatc atggcatggc tgatcaccgt actgattcgt ctggcgacgg
     6121 gtcagggtgt gg
//
LOCUS       DAJTYZ010000194         6069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000194 DAJTYZ010000000
VERSION     DAJTYZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.194"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..202)
                     /locus_tag="OO596_003837"
     CDS             complement(<1..202)
                     /locus_tag="OO596_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6719576.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCN"
     gene            complement(571..1122)
                     /locus_tag="OO596_003838"
     CDS             complement(571..1122)
                     /locus_tag="OO596_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6719577.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            1709..2056
                     /locus_tag="OO596_003839"
     CDS             1709..2056
                     /locus_tag="OO596_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6719578.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            2109..2504
                     /locus_tag="OO596_003840"
     CDS             2109..2504
                     /locus_tag="OO596_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6719579.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2545..3288
                     /gene="cmoA"
                     /locus_tag="OO596_003841"
     CDS             2545..3288
                     /gene="cmoA"
                     /locus_tag="OO596_003841"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6719580.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3285..4256
                     /gene="cmoB"
                     /locus_tag="OO596_003842"
     CDS             3285..4256
                     /gene="cmoB"
                     /locus_tag="OO596_003842"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6719581.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4421..>6069)
                     /gene="torZ"
                     /locus_tag="OO596_003843"
     CDS             complement(4421..>6069)
                     /gene="torZ"
                     /locus_tag="OO596_003843"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6719582.1"
                     /translation="DNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQ
                     FEEYLRGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQ
                     KHWMLVTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADD
                     GGMRAIPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAW
                     QKPEMIVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFE
                     ARNDFDVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFW
                     QQNKLIEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHP
                     TWLAPDEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAAR
                     FSIANGDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTA
                     DIPSSQLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1480 a         1512 c         1362 g         1715 t
ORIGIN      
        1 ggttacagaa gaccttttca ctaacccatg attgacggct tatatcatcg gtgaatcgtg
       61 gtaacagcgt gtcatgctca gttgcagcac catctagact gaaaataaat tcacggtgta
      121 agttcttaaa taacaaaaat ggagttctcc agtaatcaga acccccatga tgcttcccga
      181 ttaaaatatg ctcaattgac ataagaaata aaaattttta ttgcaatcac tcttgacaag
      241 gtttactctc ttttaagaga tgcttaacag gttttctaaa cggatatcct ttctgggata
      301 tctatctttt atcttaaaag tttttacaga tactttaaag atatctgtat atattctgta
      361 cagtaacttt tcagagtctg aaaattttta taaaaaatga ctagccctct ttgaatctca
      421 tcttccccga atcttcgaaa atatctgtct agttgattcg gtagaatcta cctcatgatg
      481 tatacgtgtt tagtacccgt cacaccagct atgtgacccc cttggcaaaa aagccaaata
      541 tcagctacat ccatgtaact attcagtaac ttatttagca agtctgtaat aagttgctct
      601 agagattcct agctcttcct gaatctgcaa cctcgtttta ccttgtgaaa taagcatctg
      661 tacagcctct gaagaagctt ttacaggtct tcccatatgt accccttcag ctttagcacg
      721 ttcaatacca gcctttctac gctctgctat caagcttctt tcaagctgtg ccaacgttga
      781 cataatgcct aacattgctt cacccatagc gcttgataag tcaaatcctt ctctaaggct
      841 gataactgtc acacctttag cctgtagcgc tttaaccgtt gataacacat caattgtgtc
      901 acgtccaaga cggtctacag ctaccacaac tacggtgtca ccttctcgtg cataagctaa
      961 caaactgctg aaaccgtttc ttaaagaagc tttgacagaa ccactgacag cttcatcagc
     1021 gaaccattta gcaatgttgt accctgcctc ttcaatttgc atttgctggt tagtgatgga
     1081 ttggtcatct gtactcaccc tgcaatatcc aagtatcgcc ataatgttcc ccttagaagt
     1141 ctcaaaagtt agtctcataa gcttagctgt ctcataatcc aaaaacaacc ttttgagact
     1201 acttgagggt gcattctacc gttacgttga aagtgtctca aaaggacttc cttatgagac
     1261 tggttaatag agcgctatga agattcagga gggtaactat aaccatcact ttctaacttt
     1321 cacgtaggaa atcgttaagt ttcttgatag ttacaccatg tgaccaacta tctatatatc
     1381 gttatatagc cctgtatata gcactatatg tagtgtctgg agagggacga ctaacacgat
     1441 acagtttacc ctgaaaagag acttcacaga ttctttatat atttcaataa gttatgctaa
     1501 aaagagacta taccgatact gcatagaatt tgtgtaggat tgcatagata cttggaaaaa
     1561 caccgttaaa ttgcatagtc attcacctga tattgtcctg tttttacagg tagttgattc
     1621 ggatgttcta ggaatcatcc tcatagttga ttcggttagt tgggcttgta ccaaccccta
     1681 atcaacctca tagtgtattt agaccttgat gattttagac agccgccctg ttcatgcagc
     1741 acgtccacac agtgaagcta ttcgcgacgc tcaacgaaaa aaacctaaag ttccggtaca
     1801 tgctgtactg acggcgacaa atccgctgat tcgttttatc ggtagtgatg atatgacgca
     1861 aaaccgggaa ttatttcagg tctggttaca gaaattagcg cagtggcatc agaccactac
     1921 gccttatctt tttttacata cgccagacat tgcccaggcc ccggaactgg tacataccct
     1981 gtgggaagac ttacgtaaaa cgcttccaga gatcggagca gttccggcta ttccacagca
     2041 atcttctctt ttctgaattt gccacctatc atagacaggt gccatcggcc attttaaagg
     2101 gagtttgtat ggtaagcgcg ctatatgccg ttttaagtgc gttgttatta atgaagttct
     2161 cttttgatgt cgttcgcctg cgaatgcagt accgcgttgc ctatggtgac ggtggtttta
     2221 gcgaactgca aagcgctatt cgcattcatg gtaacgcggt ggaatatatt cccatcgcga
     2281 ttgtgctgat gctgtttatg gaaatgaatg gcgcagaaac ctggatggtg catatttgcg
     2341 gcatcgtttt gcttgctggt cgtctgatgc attattacgg ttttcatcac cgtctgtttc
     2401 gctggcgacg ttccggcatg agcgccacct ggtgtgcgct gttgctgatg gtgctggcga
     2461 atctttggta tatgccctgg gagttggttt tctccctgcg ttagcgcaca atacgccact
     2521 ttctttttcc cggattttta cgttatgtct caccgcgaca cgctattttc tgcccctatc
     2581 gccagactgg gcgactggac ctttgatgaa cgggtagctg aagtcttccc ggatatgatc
     2641 cagcgttccg ttcccggcta ttccaatatt atttccatga ttggtatgtt agccgagcgc
     2701 ttcgttcaac ctggtacgca ggtttacgat ctgggttgtt ctctgggcgc ggcgacgctc
     2761 tcggtgcgtc gcaacattca tcatgataat tgcaaaatta ttgccatcga caactccccg
     2821 gcgatgattg aacgctgcag tcgtcatatt gacgcctata aagcccctac gccagtagac
     2881 gtcattgaag gtgatattcg cgatatcgcc attgaaaacg catcgatggt ggtgctgaat
     2941 tttaccctgc aattcctgga accttccgag cgccaggcat tactggataa aatttatcaa
     3001 ggactgaacc cgggcggcgc gctggtgctt tcggaaaaat tcagtttcga agatgccaaa
     3061 gttggtgaac tgttgttcaa catgcaccac gactttaaac gtgccaacgg ttacagcgaa
     3121 ctggagatca gccagaagcg cagcatgctg gaaaacgtga tgctgaccga ttccgtggaa
     3181 acccataaag cacgcctgca taaagccggt tttgagcata gcgagctgtg gttccagtgc
     3241 tttaactttg gttcactggt ggcattaaaa gcagaggacg ctgcatgatc gactttggta
     3301 acttttattc tctgattgcc aaaaatcatc tttcacactg gctcgaaacg ctgcccgcgc
     3361 agattgctaa ctggcagcgc gagcagcagc acgggctgtt taagcagtgg tccaacgcgg
     3421 tggaatttct gcctgaaatt aaaccgtatc gtctggattt attgcatagc gtaaccgccg
     3481 aaagcgaaga gccactgagc accgggcaaa ttaaacgcat tgaaacgctt atgcggaacc
     3541 tgatgccatg gcgcaaaggg ccgttctcac tgtatggcgt caacatcgat accgaatggc
     3601 gttccgactg gaaatgggat cgtgttctgc cccatctttc tgatttaacc ggacgcacca
     3661 ttctggatgt cggctgcggc agcggttatc acatgtggcg catgattggc gcaggcgcgc
     3721 acctcgcggt ggggatcgac cctacgcaac tgtttctgtg ccagtttgaa gcggtgcgta
     3781 aactactggg taacgatcag cgcgcacatt tgttaccgtt aggtattgaa cagcttccgg
     3841 cactgaaagc ctttgatacc gtcttttcga tgggcgtgct ttaccatcgc cgttcgccgc
     3901 tggagcatct ctggcagttg aaagatcaac tggtgaatga gggtgaactg gtgctggaaa
     3961 cgctggttat tgatggcgat gagaacacgg tgctggtacc aggcgatcgt tacgcgcaaa
     4021 tgcgtaatgt ctatttcatc ccttccgcgc tggcgctgaa aaactggctg aagaagtgtg
     4081 gttttgtaga tatccgcatt gtggatgtgt gcgttaccac tactgaagag cagcgacgca
     4141 ctgaatggat ggttaccgaa tctctgtccg atttcctcga cccacatgat ccgagtaaga
     4201 cagttgaagg ttatcctgcg cctaaacgcg cggtgctgat tgcgcgcaag ccgtaaaggt
     4261 ctggtattac tgccggatgc ggcgtgaatg ccttatccgg cctacaaacc cttgctaatt
     4321 caatctattg cagggactat gtaggcctga taagcatagc gcatcaggca gttttacgtt
     4381 tgcataacct cagcgcccgt ttccgggcgc tattcacttc ttacgcctga acagcaggct
     4441 tatcaaatgc cgttaacttc ggtgcgttac ccgtatattt ttcgatatac accagcgcag
     4501 agttaccggc acaggcattt gccagctgcg agctggggat atccgccgtt aacacgttcg
     4561 cactgccgtt tttacacaag ccgttttcca gatctggcca tgcaccttca tgcacgcata
     4621 ccacgccttt tttgatcccg tcagtcacca ccgcgcctgt caaaatctga ccacgtttgt
     4681 tccagacacg caccagatcg ccattcgcaa tactaaatcg agcagcatct tcggtgtgaa
     4741 tagtgatcgg ttcacgatcg gcaaccgcat attttttacg cagttccgca tagttaagct
     4801 gactgtgtaa acggtgtgcc ggatgtgcgg tcagaagctg caactgcttc tcgtcggcgg
     4861 tacccttcca ctcatcaggc gcaagccagg ttgggtgtgc cgggcaatcc ttatagccaa
     4921 atttttccag cgttttggag taaatctcaa ttttgccact tggcgtaccc agcgcatttt
     4981 tcaccggatc ggcgcggaaa tcaccataac gaacgtactg ttcgttcttc tcgctgcggc
     5041 gcatttcgat cagtttattt tgctgccaga aggcattaaa cattggcata gtgacgcgtt
     5101 gcgcacgggc ccctttctga gcggcatcat agaaaaattt cagccacgcc atttcatctt
     5161 taccttcggt atagatctct tttccgccag gtttgagtaa ttccgcaaga tcggcaaaca
     5221 catcaaaatc gtttcgcgct tcaaattgcg gagcaacagc ctgcttcatc ggcacaatat
     5281 gctggttgct gtaatcaccg gtcatcgtca agtcattgcg ctcaaacgat gtggtgatcg
     5341 gtaatacgat atctgcgtgt ttagcagctg cggtccagta gcattcagaa acgacgatca
     5401 tctccggttt ctgccatgct ttaatcagac gattagtatc ctggtgatgg gtaaagttac
     5461 cgccacccgc ccaccagatc attttgatat tcggatacgt ttgctcttta ccgttatgct
     5521 gatatttgcc gccaggattt tccagcgcat cgacgatacg ggcaacagga atcgccctca
     5581 ttccgccatc atcagcggcc tcgctggcct gtccggcaat cgccgcagac atttccggca
     5641 gcacgccgcc aacacgtgtc gggttgccac cattggagta atgataagag aaaccaaaac
     5701 cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag cgtcaccagc atccagtgtt
     5761 tctgttcacc atattgctgg cgctgaattc cccagcctgc catcagcatt gtgcgattcg
     5821 ccgccatcag ttcagcgagt ttgactatct gcgcttccgg tacgccagta atttccgccg
     5881 cccaggccgc gctcttcggc gtattatcgc tcttacctct cagatactct tcaaactgcg
     5941 gatacccggt agtgtatttt tcaaggaaca ctttatcgtg tttgccttgt gtcatcaggg
     6001 tatgcgcaat ccctaacatc agtgccacgt cggtgcccat attcggcgcg atccaggtgg
     6061 cattatcgc
//
LOCUS       DAJTYZ010000195         6037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000195 DAJTYZ010000000
VERSION     DAJTYZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.195"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..330
                     /locus_tag="OO596_003844"
     CDS             <1..330
                     /locus_tag="OO596_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6719583.1"
                     /translation="GLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACC
                     GHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQSGRPLKQEPT
                     EMIQATA"
     gene            complement(370..1041)
                     /locus_tag="OO596_003845"
     CDS             complement(370..1041)
                     /locus_tag="OO596_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR6719584.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1148..1381)
                     /gene="yedF"
                     /locus_tag="OO596_003846"
     CDS             complement(1148..1381)
                     /gene="yedF"
                     /locus_tag="OO596_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6719585.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1378..2583)
                     /gene="yedE"
                     /locus_tag="OO596_003847"
     CDS             complement(1378..2583)
                     /gene="yedE"
                     /locus_tag="OO596_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6719586.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2770..3183
                     /locus_tag="OO596_003848"
     CDS             2770..3183
                     /locus_tag="OO596_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6719587.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3217..4704)
                     /gene="amyA"
                     /locus_tag="OO596_003849"
     CDS             complement(3217..4704)
                     /gene="amyA"
                     /locus_tag="OO596_003849"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6719588.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4782..5147)
                     /gene="fliT"
                     /locus_tag="OO596_003850"
     CDS             complement(4782..5147)
                     /gene="fliT"
                     /locus_tag="OO596_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6719589.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5147..5557)
                     /gene="fliS"
                     /locus_tag="OO596_003851"
     CDS             complement(5147..5557)
                     /gene="fliS"
                     /locus_tag="OO596_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6719590.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5572..>6037)
                     /gene="fliD"
                     /locus_tag="OO596_003852"
     CDS             complement(5572..>6037)
                     /gene="fliD"
                     /locus_tag="OO596_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6719591.1"
                     /translation="GDSVVRTIQSGIRAQFANGASDSAFKTLNEIGIKQDGTTGKLKI
                     DDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGYLADDGIIDSAQDSINATL
                     KKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTSTYLSQQFTAMSNS"
BASE COUNT         1479 a         1588 c         1508 g         1462 t
ORIGIN      
        1 ggtttaaacc gttcgatact ggatcagggc tggtatgaaa tgcgccgcca gcttgagtac
       61 aagcagctct ggcgtggcgg tcaggtgctt gctgttccgc cagcgtacac aagccagcgt
      121 tgcgcgtgct gtggtcatac agcgaaagaa aaccgcctgt cacaaagtaa attcagatgc
      181 caggtatgtg gatatacagt gaacgccgat gtaaatggcg ctcgtaacat tttagcggcg
      241 gggcacgccg ttcttgcctg tggagagatg gtgcagtcag gccgcccgtt gaagcaggaa
      301 cccaccgaaa tgattcaggc gacagcctga acgtagcagg gatcctcgtc cttcagggcg
      361 tggaggatgt caacaaacag gttgaattaa ctccgcgccc tggtttttga cattacctac
      421 cgcgcgcgat accgggtgcc aggtgaactg gtttgctggc acacagccgt tggtagcaat
      481 ttctgatgct tctttaccgc caatctcttg ccgcatccat tcctgtgcag cttctggcga
      541 cagaaccagc gggcggcggt catgaatgtc taccagaccc tggtctgctg cagcagtgac
      601 aattaaaaat ccttcggctt cgtcaccacg ctcaaatggt gtgctaccta tcgcggccat
      661 aaaaataggt tgtccatcag cacgatagat aaaaaaaggc tgttttttgt cgccttcttt
      721 tttccactca aaccagccat cggcaaaaca gattgcccga ccatgttgcc agagcggttt
      781 aaacatgcga ctggttgcgg cagtttctac gcgggcgtta atcagcggcg gtttatccca
      841 ccatccggga gcatatcccc agaaaaccgg atccagatga aggtgttcat cacgttcact
      901 gagcagaaga actttggttc ccggcgcgac gttgtatctg ccaatgggtt cgggatcgta
      961 gggaatatcg cgttcaatat cttccgcgag aagcgcaagg taatcttcac gcgtttggga
     1021 ttgggcaaag cgtccacaca tagaaacctc ctgagatcat actgaaaaga ataggagagg
     1081 gggcgggtaa aatctatgtg tcagaggcaa caatgttccc gctgtgggcc acagcgggaa
     1141 tgagggatta cttctgaatt aaataacgaa tggtcggccc gtcttgctga atatccagca
     1201 ccgtatagcc gtgattacgc gcatccagtg gaatattatt gatcgactgt ggacagtcgc
     1261 tcaccacttc caggatttcc ccttttttta actgcggcat cgcctcaagg gttgcgactg
     1321 ccggataagg gcagggttca cccaccatat caaggcggta atcaggaacg atatttttca
     1381 tgcgatctcc ttagcagtct gcggtgccgc acggcggaag aagcgttttt cccagccgat
     1441 aatcaacatc agcgcagtaa acaacagcaa atatgtcacc agcaggccac ccatcgggcc
     1501 aaaggttttc agcaggttga ttttgtccca gtcggtggcc agcgccggag cgaaatcatc
     1561 ccagtaatac gccagaatcg tcgagccgat cacattgccc agaccgaccc accagtagtg
     1621 cacctggcct tctaccgcgc ggtacatcca gccggtttcg cagccgcctg ccagcacgat
     1681 gccaaaacca aacagtaaac caccaattac cgcgtttggt cccgcccaca tgattttggg
     1741 ttcaacgcct aactgtacgt aactgaagat cccgatggca ctcaccgcca taccgataat
     1801 gattgctttc gccatatggg tacgtccggt gatccacata tcgcggaacg ccgaagtaaa
     1861 gcagatttgc gcacgttcaa tcagtaaacc aaagccgacg ccaaacagca ttgccagccc
     1921 cagtttgggt tggttcatcg ccgtgagcag cgcccagccc agcatgccga aaaagaccag
     1981 catcccgaga cgaaaacgac gccgcgcctg atccggtttt tgcgtcagcg gtgaggcggc
     2041 agaaactttc tgcattttca cgggaatacg gaagatgggc agcagggtaa agcgcgcgcc
     2101 aaaccatgaa ccaatggcag tggcgatggc aaagaaccag gcatgcagcg agaactgagg
     2161 aatgccggta aagaacgccg ccaggttaca gcccattgcc agacgcgcgc caaaaccggc
     2221 gataatgccg ccaataatgg cctgcataat gcggatacgg ctgcgcggca tgcgcagttt
     2281 gacattgttg gcccacagcg ctgcggcaaa gcagccgcca aacatgccga ggatcatcat
     2341 cccgtcgatg cgggttaatg gcgatccttc cagatggata attttaaaat aaccccactc
     2401 ttcagcatgg acgccaaaca gctgcaggag ctgaccgccc caacgggtaa attcacccgt
     2461 gacagcccaa aaggtgccag taatgccaaa atagtaagtg gagagaatac ccgccgcgat
     2521 gaccgcaggg atgggtgccc agaatttaat caaccaggcg tgtttgaatt gctgccatga
     2581 catgaatgta gcctctgaac tcagaaggtt tgaaacaaga atcgcgaatc atacatcatg
     2641 caaatttatt ttcggggtaa taattcacaa aaataatagt tagatcaaat ttctctctga
     2701 acctggaaag gctggcgaca tcagggcttt aatgccacaa tcctcttttc ttctcatgca
     2761 ggacgaatga tgaaaaaact cgcaattgca ggtgcgctca tgttgctggc gggctgtgcc
     2821 gaagtcgaaa actataacaa tgtcgtgaaa acccccgcgc cggactggct ggcgggctac
     2881 tggcagacca aagggccgca acgtgcgctg gtcagtccgg aagagatagg cagcctgatt
     2941 gtcactaaag agggcgatac cctggattgc cgtcagtggc agcgggtgat tgccgtgccg
     3001 ggcaagttga cattgatgtc tgatgatctc actaacgtta ccgtaaaacg tgagctgtac
     3061 gaggttgagc gtgacggtaa caccattgag tacgatggta tgacgatgga gcgtgtggat
     3121 cgcccgacgg cagagtgtgc cgccgcgctg gataaagcgc cgttaccgac gccactgccg
     3181 taatgaaatc tccccggctt gccgccgggg atgaatttaa atcacctctt cgataaccca
     3241 cacgctgacg ctgccgccgt tgcaaaagaa ggtcgcttcg ccgttttcgt cggtcactac
     3301 gctctcttgc cgattcccca gaaaatcacg ccaggtttta ttgccgtaat tctctcccag
     3361 atgaatggtt ttttcgccat catccccgtt cgacatgacc accacgcagc cgggatattc
     3421 gtcagtgcca ctgcggctaa aggcaatgca gttcggatgg tcgaaaaata acgtctgtac
     3481 accgtgggcg aaacgctgac gggcgagaat taactcatca agctgttcga ttattggcat
     3541 atctatcgga taggtttgcc cgtcaccacc gacatcttcg taatgcgcac cgtagaggtc
     3601 cggatagaac accgaaggaa cgccattttc ccgcaacaaa attagagcat acgccagcgg
     3661 tttaaaccac ggttcgaccg gcgcttcgag ggcttgcaac ggttgggtgt cgtgattggc
     3721 tacgagggtt acggcgtgga aaggatcggc ttccactaat gtacccgtga aaatctgcgt
     3781 catgtcgtag tcgcgtccca tgcgcgatgc ttcatggaat ttcatctgca gcggcgcatc
     3841 gaacagcatg gttttgcctt ccacctgatc aatatacgtt tgcagtttat cgacttcatg
     3901 cgaccagtac tctgccacaa taaacagcgg ctttggcgca acttcctgta cgtgttcgat
     3961 ccactcttta taaaaccacg ccggaatatg tttaaccgca tcaagacgaa aaccgtcgca
     4021 ttgtgtttgt tccatcaccc agcgcgccca gtatttaatc tcttccgtca cggcatgatt
     4081 gcggaaatcg atattctcac ccatcagata atcgaaatta ccgagttcat catcaacctg
     4141 atcgttccag ccttcgccgg tgtagtcgtt aacaatttta aatatgccat cttcatcagg
     4201 attttcgata tggtcgatac cgctaaaaca tttgaagtcc cagataaact gcgagtattg
     4261 cccggcacgg gcggggaagg tgtaacgcgt ccagccttcg cactcaatga tttcttcatc
     4321 aatttgcgta cggtcatcag catttacacg ctgcacgcga atagcttctt tttcatccgc
     4381 ccccattttg tggttgacta ccacgtccag tagcaccgca atgtcattac gtttcagagc
     4441 gtcaatggcg gccagcagtt gtgctttatc gccatattta gtagggatgc tgcctttctg
     4501 atcaaactca cctaaatcaa ataaatcata ggagtcgtag ccgaccgaat acccgcccga
     4561 tgcgccttta taggcgggcg gcaaccagac catattgata ccaatatcat taaaaccgtc
     4621 ggcgcgctcg gccagttcag gccagagctt accgccttcc gggtaatacc agtgaaaaca
     4681 ttgtaacagc gtgggattac gcattcttcg actccattca aggggaacat tagaagcgta
     4741 gccgtaatcg gattattcgc gagccatcgg ctcgttaaga ttcaaaagag gttatcctgc
     4801 ggagccagca caaagccgcc ctgatctcca taggcactta acactgattt ttgcaccgat
     4861 gactgaccga ccagtttcgc cagttcatcc atacgaatct gtaataactg ctttaccttg
     4921 ctttcgttgt cgagaatcag gcgcagcatc gggcggagct gctcctgcat cgtggtggac
     4981 ggggcaacct cttcagtcag atgtgcaatc tcctgcaccg cattcacata cgccatttcg
     5041 ctggcgatga gttcgtccca ttgttcttcc gttgccaggc gtaacatgag ctggctttta
     5101 tcgacgagtt gttgccaggc gaaatacaaa tgcggtgcat ggttcatcag actgggtcct
     5161 gaatcaaaga aggggagagt aaagactctt tccaggcatc ggcaatattg cgcattaatg
     5221 cttccacttc ttcgactgcg gagacatcgt tgcgtaaatt ggcttgcagc aagcgcctga
     5281 ccatatagct ataaagagca atcaagtttt gggttagttc gtctttgctc tcttcatcaa
     5341 gactcacccg cagcccgttc tcaatgatgt tgatcgcttt tgacaaagag acgcctttgc
     5401 cttgctgatt gttgtcctgc ataaacaggc gcgctctaac cagtgcgctc agcactccat
     5461 caaatagcat ggtgaccagc tgttgctggc tggcgctcat tacggcgctt tcgacgccaa
     5521 tttgtgcata ggcttttgag ccgctcgcgg tatacatgct gacctccgtg atcaggaatt
     5581 actcattgca gtaaattgtt ggctgagata ggtactggtg ttattcagtt tgctcatcat
     5641 ggtatctaat tgcgtaaact gtgcagtgta acgcgccacg gtgtcatcaa tgctggcact
     5701 gacggataga tactgtttgg tcagtttttt cagtgtggcg ttaatactgt cctgtgcgct
     5761 atcaataatg ccatcatcgg caaggtaacc ttttacctcg gtggcaattt tggtggtaat
     5821 tcctgtttct ttaccatcgc ctaccagcaa ttcacgtacg gatgctgtat tttcattcag
     5881 gacttttttg agtttatcat catcaatttt cagcttaccc gtcgtgccgt cctgcttaat
     5941 cccgatctcg tttaatgttt tgaaggcact gtcgctggca ccgtttgcaa attgtgcgcg
     6001 gatgccgctt tgaattgtac gtacaacgct gtctccg
//
LOCUS       DAJTYZ010000196         5994 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000196 DAJTYZ010000000
VERSION     DAJTYZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.196"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..545
                     /locus_tag="OO596_003853"
                     /pseudo
     CDS             1..545
                     /locus_tag="OO596_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(587..1039)
                     /locus_tag="OO596_003854"
                     /pseudo
     CDS             complement(587..1039)
                     /locus_tag="OO596_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1103..1249
                     /locus_tag="OO596_003855"
                     /pseudo
     CDS             1103..1249
                     /locus_tag="OO596_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1249..2406)
                     /gene="intS"
                     /locus_tag="OO596_003856"
     CDS             complement(1249..2406)
                     /gene="intS"
                     /locus_tag="OO596_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6719592.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2568..2642)
                     /locus_tag="OO596_003857"
     tRNA            complement(2568..2642)
                     /locus_tag="OO596_003857"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2607..2609),aa:Arg,seq:cct)
     gene            complement(2718..3650)
                     /locus_tag="OO596_003858"
     CDS             complement(2718..3650)
                     /locus_tag="OO596_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6719593.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3944..4699
                     /gene="mlaA"
                     /locus_tag="OO596_003859"
     CDS             3944..4699
                     /gene="mlaA"
                     /locus_tag="OO596_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6719594.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4881..5216)
                     /locus_tag="OO596_003860"
     CDS             complement(4881..5216)
                     /locus_tag="OO596_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719595.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(5174..5566)
                     /locus_tag="OO596_003861"
     CDS             complement(5174..5566)
                     /locus_tag="OO596_003861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719596.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5573..5949)
                     /locus_tag="OO596_003862"
                     /pseudo
     CDS             complement(5573..5949)
                     /locus_tag="OO596_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1495 a         1341 c         1320 g         1838 t
ORIGIN      
        1 ttaagggata tttctgattg tagggaatat ctatcttatt taaaaataaa ctttgctgac
       61 ttcagtttaa ttaaaggatt aattagttgg gctaatcact gtgctgaata catagaaatt
      121 tttgatgagt ctattgcttt tacatgtctc tctgcatttt cttctgaaaa acaatttgga
      181 gtatttctgt ttggatgttt gaaaagcaca gggctaaagt taaaacgatt attcatacgg
      241 atttatctgc accatggcgt cttaaggata tatcatcaag attatatctc agcgaaagtt
      301 tactaaagat gaaattgaaa gaagaggggg tatcattcag taagatcata cttgatgaga
      361 ggatgcaaat ggctgaatat ttactcagca ctcgttgtta tcctattagt aaagtagcta
      421 aggtctgtgg ttatgccagt gtctcatact ttacttatgt atttagacgt tattttggtg
      481 tttctccaag tcaatactct cagaggagtt cagaaagtaa aattcttact caccagggaa
      541 tctgatcatt gttcttgccc ccttatttcc agacaggggg tgtatcttaa gttaacgtta
      601 cccgctgacg tcgatattct cgcggagagc gataacccag cgcactgtgc ggatggtttt
      661 cattgtaatg ttcgatcgcc actgcaagat tatgcaatgc cgttcttaca ttcggtttcg
      721 gcatgaacgc gatgtagtct tccttcatcg ttttcacgaa cctttctgcc attccattac
      781 tctgaggact gctgattgcc gttgtgcagg gctccagatt caactctctg gcgaactgcc
      841 gtgtttcatg cgctctatac gctgaaccgt tatctgtcag ccactggatg gactgttccg
      901 gtactttatc gccaaagcgt ttttctatcg ctcctaacat gacatcctgc actgtcgctt
      961 tatcgtaacc tcccgtgctt gcggcccagt ctatggcttc acgatcgcaa cagtccaggg
     1021 cgaacgtaac acgcagtttt gaatcgccac gggtttaaca gacacctcag agtcatttaa
     1081 gatggcttaa agagaggtgc ccatgagcgg taagcgttat cccgaagagt ttaaaactga
     1141 agcagtcaaa caggttgttg atcgcggtta ttctgttgcc agcgttgcaa cacgtctcga
     1201 tatcactacc cacagccttt atgcctggat aaagaagtac ggtccggatt actccacttt
     1261 ttcatcaagc caatccgccc accactgcat catttctctg cgcttatcga gatactgagc
     1321 atggttgtaa ataccgcgca cagatccgcc gttggcatgt gccagctgca cttcaatagc
     1381 gtcagcaggc cattcgtgct cgttcataat cgtgctgaat tcatgcctga atccgtgacc
     1441 gctttccaga ccttcatagc cgatttgttt gatcacaagc aataccgcgt tctcgcagat
     1501 tggcttcttc ttatcgttgc gcccggcaaa aacaaactct gagactggtt tggtgattga
     1561 gcttagcgta gtgagaagtt caaccacctg gtctgacatc gggaccacat gaattttgcg
     1621 tcccttcatc acactggcgt cgatggtgat aatcctgttt tcaaaatcga cgttcttcca
     1681 tagcatggaa cgaagctctt ttgttcttag ggctgtgtag cgtaaaactt tggtcgcaat
     1741 gagcgatacg atacttcctg aaaatgttgc cagtgctttg ttgaatgcag gcatctggtc
     1801 tgctggaagg aacgggaagt tcttcttgcg gtatcctttc atggcgtctg caaggtcagg
     1861 tgccgggtta tatttagccc ttccggtgac aatagcgtaa cgaaaaacct cgccgcatct
     1921 tctgcgggct ttgttggctc gctccattgc accgcgatct tcaaatctgc ggattacttc
     1981 cagcagttgc atcggctcaa tatcctgaat ctcaagaccg ccgatgatgg gtaaaatgtc
     2041 gtcatcaaac attttggcaa gttcagttgc atagcctact gaccagactt gcttcttgtg
     2101 ctcgtaccat tccttgtaaa tcgcactaaa tgaattgttg ttagacgaag cctttttcgc
     2161 ctttaccgga acgatgccaa ccgagatgtc tttcctcgca gtccatgcct tatcccttgc
     2221 ttcctgcaaa gtcataagcg gatattttcc gacggtcagg attttctcct taccgtcaat
     2281 cttgtagcga agctgccata cctttttccc ggatacagga acataaaggt acaggccatt
     2341 accatcgaga aggcggtatg gtttttcttt cggctttgct gcttcaatct gcttaacggt
     2401 gagcatgggt aaaaatccgg tgggtaaaat tattttatcc actttttacc cgtcatggag
     2461 tgcggctgtc aacgatctga cgcgaaccat gacgaaccgt gaatatacgg aaggcttgat
     2521 attcagggga ttttgcggac tggtacggat gggagcgaac tgataaatgg tgtcccctgc
     2581 aggaatcgaa cctgcaatta gcccttagga ggggctcgtt atatccattt aactaagagg
     2641 acaatgcggc atgagtatac ccgctaatgg agtgcggggt aagtacgctg ccgctcgatt
     2701 gcttaaaccc tcgccattta tgccgggttt ttatcatttt tcttaatgtt ttccgcacgt
     2761 tctgcttttt ggcgtgcttc tgctttacgc ttgttgctca tgtcgttacg aatctgtgca
     2821 tgactcatta acgcgaagat aaaggtgccg ccgcagatgt tccccgctaa agtaggtagt
     2881 gcgaagggcc agatgaaatc gctccagtgc agcgtaccgt taaacaccag atagaggatt
     2941 tcaacagaac cgaccacgat atgggtggtg tcacctaggg caataagcca ggtcatcaat
     3001 ataatcacca caatctttgc cgcacctgct gcaggaaaca tccataccat agtggcgatc
     3061 agccagccgg aaatgatcgc gttggcaaac atctcgctgg gggtgttctt catcacatcc
     3121 atgccgattt tgacaaatgc atcgcgagtt tcttcattga agataggcat atattcaaat
     3181 gcccacgcag caataccagt cccgagaata ttacccagca gcacgactcc ccataaccgc
     3241 ataagtaagc caacgttgct cattgtcggt ttttgcatga cgggtagtac cgcagtcacg
     3301 gtgttttcgg taaataattg ctggcgggcc attatgacga taataaaacc aaaggtataa
     3361 ccgagattct ccagtaagaa gctgcccggc acaccttcca gttcgacatg aaatatccct
     3421 tttgccagta acgaagcgcc catcgacaga cccgccgcaa tggctgacca cagtagcgcc
     3481 attgcgtcgc gttccagctc tttttcacca tcctggcgga tatgctcatg aattgccatc
     3541 gcccgggagg ggagtcggtc ttcatctatt tctatttttt tgccgcgctc tttttcttcg
     3601 ctctcaactt caatttcgtc gctgtgttga tcaattttgt cgttgtccat ggtctcttct
     3661 cttgaattag cacgtatagc taaagcgtag cggctttttt gctcgcaact ggcgggagtt
     3721 actctgaaaa tgtagaaaag gctgcgtttg cctttttctg tttctataga atcaagtagc
     3781 ctacagggcg gcgattacca ggctatgatc aaatcagcaa atcagggcgt ctggacatca
     3841 gttgacgtgc tgttacaatc gcccacacct aaacaggcgg atacggtatc gttccgtcat
     3901 ggatggcaaa ctgcataagc cataaaaaaa cagggagaca tttatgaagc ttcgcctgtc
     3961 ggcgcttgct ctgggaacta cgcttctggt ggggtgtgcg agttccggta cagatcagca
     4021 agggcgttct gacccgttag aagggttcaa ccgcaccatg tacaacttca acttcaacgt
     4081 attagacccg tatattgttc gaccggtcgc tgtcgcctgg cgtgattatg ttccgcaacc
     4141 ggcgcgtaat ggtttgagca actttactgg caaccttgaa gaacctgcgg tgatggttaa
     4201 ctacttcttg cagggcgacc cttatcaggg gatggtccac tttacccgct ttttcctgaa
     4261 caccattttg gggatgggcg gctttattga tgttgcaggg atggcgaacc cgaaactgca
     4321 acggactgaa cctcaccgct tcggtagtac gcttggtcat tatggcgtgg gttatgggcc
     4381 ttacgttcag ttaccgttct acggtagctt cacgctgcgt gatgacggtg gtgatatggc
     4441 ggatggtctt tacccggttc tttcctggct gacctggccg atgtctgtgg gtaaatggac
     4501 gcttgaaggg atcgaaaccc gcgctcagct gctggattcc gatggtctgc tgcgtcagtc
     4561 gtccgatcct tatattatgg tgcgcgaagc gtacttccag cgtcatgatt tcatcgctaa
     4621 tggcggcgaa ctcaaaccgc aggaaaatcc gaacgcacaa gcgattcagg atgatttaaa
     4681 agatattgat tctgaataag aaacaaataa aaaaggtgag tcgcaaaact cacctttttt
     4741 gttgttatcc ctcaattacc acaagtaaaa ggagataaac cgacaatgac ccggacgtaa
     4801 tgatgtaaaa atactgatgt tcgtcactga ctatttccga tattcaggcg ttgaaaaatc
     4861 aaaaaactgg aaaaataatt ttaaatatca tcaaaattaa cacctggcgc agatagttga
     4921 ttaatttcat tttgatattt cgcctggcga ttttgaagtc ttttttcaac gttatcttcg
     4981 agatccttaa agtctttgta tttattttca ggatggtcaa cgtcaacttt gttagcgaat
     5041 aaattagaaa ttatttcttt tacattgtcg cgtctaaatt ttgcatcgtc cacttgaaaa
     5101 ttatatctta ttgctcgacc taaacgttta aaaacttcgt taggttctct cagtctttcc
     5161 ccgttgagct cgtttacaac ctgatgatcg gaacctgttg caaatgattc tgccatgata
     5221 gctttactgc tttcaaacca cgctgaagca ttgctgaagc attcctggct ttttcactac
     5281 aatattgggt gagactatca ccattaatac gaattcctct ttgaatgtgg tagtcgtcct
     5341 gatcgtagta accgagagat aagtaatcat ttccgttttt atttttaatt acaatagcga
     5401 gagagttgcc ttttttatct ttaccgtacc aggtaaataa gtccgttggt tcgctattcc
     5461 cgctctcatc aaattctgtt accgtatgtc tgagtttgtg tgatatctta tcgaagataa
     5521 aaccaggatc tccatcatat atttctttta atgcgctgaa gatcatttcc ttgttattga
     5581 tatcatgagt cttctcaact atgttgtcat taatgatagt acggacgagt tctttgtctt
     5641 tatagttcgc aggtaattct attggttctt ggttgtcgat accaatagta atatggtctg
     5701 tatcattgga gccaatatca atataagtaa ctgaggaatt attaatatta aatcttaagg
     5761 atcgtcgttt actcgtatgg cgttttttat aagctcaagt agatgtttaa tacaggtgct
     5821 tattccttcc ttaccattaa aaaggttttt gagctcttgt actaatccat taacttcatc
     5881 attgttattt tcattaatgg attctgggta agttgcgctg gtattgttga ttgaaataga
     5941 tggtagcatg attttattcc attttgattg atttgttaca aatatatatt acct
//
LOCUS       DAJTYZ010000197         5951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000197 DAJTYZ010000000
VERSION     DAJTYZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.197"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            282..358
                     /locus_tag="OO596_003863"
     tRNA            282..358
                     /locus_tag="OO596_003863"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Arg,seq:tct)
     gene            complement(403..1164)
                     /gene="envY"
                     /locus_tag="OO596_003864"
     CDS             complement(403..1164)
                     /gene="envY"
                     /locus_tag="OO596_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6719597.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1347..2237)
                     /locus_tag="OO596_003865"
     CDS             complement(1347..2237)
                     /locus_tag="OO596_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6719598.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2238..5210)
                     /gene="nfrA"
                     /locus_tag="OO596_003866"
     CDS             complement(2238..5210)
                     /gene="nfrA"
                     /locus_tag="OO596_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCR6719599.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5197..5928)
                     /gene="nfrB"
                     /locus_tag="OO596_003867"
                     /pseudo
     CDS             complement(5197..5928)
                     /gene="nfrB"
                     /locus_tag="OO596_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1352 a         1563 c         1554 g         1482 t
ORIGIN      
        1 aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc tcataggagc
       61 ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac gggttttctt
      121 gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa tcagaaaatc
      181 agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca gcaagggttg
      241 accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta gctcagttgg
      301 atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg gcgcgccatt
      361 tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca tcactttttg
      421 tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa aaacagagat
      481 aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat ttttgttatc
      541 catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct ggctatagct
      601 gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata gcgaactggc
      661 gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc agacgctgtc
      721 gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt gctcaagaaa
      781 gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg gtttgctggg
      841 ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc tatgacaagc
      901 gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct tatttaaaaa
      961 caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt gatttaacgt
     1021 tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt tcggaagggt
     1081 aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga tcaccacgca
     1141 aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact ccgctcaaag
     1201 gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt ctttctttag
     1261 cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag gtaagccagg
     1321 tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc gacgggcaaa
     1381 tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc taacagcgag
     1441 gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc ggcgacaaac
     1501 agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct ggcctgtaga
     1561 taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg tacccagaca
     1621 ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg cgacatgttt
     1681 ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat cagccgctgc
     1741 gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca gttcaggtcg
     1801 tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc gctccataat
     1861 ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag cgctgcggac
     1921 gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat cagtttcagg
     1981 ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc tggctgggta
     2041 aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg caaacgtaac
     2101 tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt ttgtggttgc
     2161 cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt cagcaatacg
     2221 aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt gttgcgctct
     2281 ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag actgactttg
     2341 tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac cccgactccg
     2401 ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac gtgagcgtaa
     2461 ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata atctgccgtc
     2521 cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt ttgggcaaac
     2581 cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa gaatgaggcg
     2641 ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa ctgctgttcg
     2701 acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt gccggacatc
     2761 ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac gcggctataa
     2821 actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc ggcttccagt
     2881 tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt gttagcggta
     2941 ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca gcggcgaccg
     3001 acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg ggtcagtggg
     3061 gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg ctgcgtcgca
     3121 ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc cggatcgtcc
     3181 ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc accgctatcc
     3241 cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag ttccagcgcg
     3301 gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat tgtcgcccgc
     3361 gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc gttcagtgcg
     3421 agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag ggcattgctt
     3481 cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc atttcctgcc
     3541 gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg aagactgatt
     3601 ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg ataggctacc
     3661 gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc atacaacgcc
     3721 acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc agcatcgtag
     3781 gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc aatacccggc
     3841 aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa aatcgccacc
     3901 ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg cgccattaaa
     3961 gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa atcggcagct
     4021 tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg ggtaagattt
     4081 gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt gttacgggtc
     4141 gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt ggcgagcagt
     4201 ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc gccaacaaca
     4261 tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac ggggttggcg
     4321 ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt aaacagcggc
     4381 ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc cagcgcggtc
     4441 gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag tgctaggatc
     4501 cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc tgccgcgctt
     4561 aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca ttgtttcagg
     4621 tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga tgcagcaaac
     4681 gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag ggcattctgc
     4741 ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg ctgggcaagc
     4801 agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag actgcgctca
     4861 agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg cgcccggtca
     4921 tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat attatccggc
     4981 acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt atttttctgt
     5041 gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc gctcagcccc
     5101 agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa taaagacgtc
     5161 agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat tttcggactc
     5221 cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt gcatcgaaac
     5281 ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga taacgccttc
     5341 ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata acaacacgtt
     5401 aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca ggaactgatg
     5461 cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact gatgctgaac
     5521 cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc agtgacgtaa
     5581 ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc cgactttacg
     5641 cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa ttaactcatc
     5701 attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg gcatttcggc
     5761 aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc cgttttgctc
     5821 tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca ttgaaccgcc
     5881 caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttcgg taatgactcc
     5941 aacttattga t
//
LOCUS       DAJTYZ010000198         5749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000198 DAJTYZ010000000
VERSION     DAJTYZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.198"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            17..355
                     /gene="spa13"
                     /locus_tag="OO596_003868"
                     /gene_synonym="spaM"
     CDS             17..355
                     /gene="spa13"
                     /locus_tag="OO596_003868"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6719600.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            342..1220
                     /gene="spa32"
                     /locus_tag="OO596_003869"
     CDS             342..1220
                     /gene="spa32"
                     /locus_tag="OO596_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6719601.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            1214..2095
                     /gene="spa33"
                     /locus_tag="OO596_003870"
                     /gene_synonym="spaO"
     CDS             1214..2095
                     /gene="spa33"
                     /locus_tag="OO596_003870"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6719602.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            2100..2750
                     /gene="spa24"
                     /locus_tag="OO596_003871"
                     /gene_synonym="spaP"
     CDS             2100..2750
                     /gene="spa24"
                     /locus_tag="OO596_003871"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6719603.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            2765..3025
                     /gene="spaQ"
                     /locus_tag="OO596_003872"
     CDS             2765..3025
                     /gene="spaQ"
                     /locus_tag="OO596_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6719604.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            3025..3795
                     /gene="spa29"
                     /locus_tag="OO596_003873"
                     /gene_synonym="spaR"
     CDS             3025..3795
                     /gene="spa29"
                     /locus_tag="OO596_003873"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6719605.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            3802..4830
                     /gene="spa40"
                     /locus_tag="OO596_003874"
                     /gene_synonym="spaS"
     CDS             3802..4830
                     /gene="spa40"
                     /locus_tag="OO596_003874"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6719606.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            4843..5085
                     /locus_tag="OO596_003875"
     CDS             4843..5085
                     /locus_tag="OO596_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719607.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         1824 a          823 c         1061 g         2041 t
ORIGIN      
        1 ctctttgtat cgagatgtgg aggcattaga taaaaggatt atatacttcc tgcaactgga
       61 gaatgactta gaacctgtag gcgctcagag tgttagtcag cttttcaata caagacgtaa
      121 gatcgctata gtaaaaaagc atattatcca atatcagtct gaacgaattc ttctaaaggg
      181 aagaattgaa gaaattcaaa aagatattga tgaggcaaat gcttctaaaa gaaaactgct
      241 tcataaagag agtaaaatat gtaaacggat tggcttgatt aaaagaaata actttgccaa
      301 acagttgata cttgatgagt tatcacaaga ggatatgaag tatggcatta gataatataa
      361 acctaaattt tagtagtgac aaacagatag aaaaatgtga gaaactatct tcaatagata
      421 atattgatag tctcgttttg aagaagaaga ggaaggttga aattcctgag tactctttaa
      481 ttgcatctaa ctattttact attgataagc actttgaaca taagcatgat aaaggagaaa
      541 tttatagtgg cattaaaaat gcgttcgaac ttagaaacga acgagcgaca tattctgata
      601 ttccagaatc aatggccatt aaagaaaata ttttgatacc agatcaagat ataaaagcaa
      661 gggaaaaaat aaatatcggc gatatgaggg ggatcttttc atataataag agtggaaatg
      721 cagacaagaa cttcgaaaga agtcatactt cttctgtaaa ccctgataat ctgctagaat
      781 ctgataatag aaatggtcaa attggtttaa aaaatcatag cttgtctatt gataagaata
      841 ttgctgacat catttcttta ctaaatggaa gtgttgctaa atcatttgag ctgcctgtaa
      901 tgaataaaaa tactgcagac ataaccccat ccatgtcatt gcaagaaaaa tcaatagttg
      961 aaaatgataa aaatgttttt caaaaaaata gtgaaatgac ttaccacttt aaacagtggg
     1021 gggctggaca ttctgttagt atttcagtgg agtctggttc ttttgttcta aaaccgtcag
     1081 atcaatttgt aggaaataaa cttgacttaa ttttgaaaca agatgctgag ggtaattata
     1141 gatttgatag cagtcaacat aataagggga ataaaaataa tagtacagga tataatgaac
     1201 agagtgaaga agaatgctaa gaattaaaca ttttgacgct aacgaaaaac tacagatttt
     1261 atatgcaaag caactctgcg agcgtttttc gattcagaca ttcaaaaata aatttacagg
     1321 cagtgaaagt ttagtcactc ttacttccgt gtgtggggat tgggtaattc gtattgatac
     1381 attatctttt ttgaaaaaaa aatacgaggt attttcagga ttttctacac aagaatcttt
     1441 actgcattta tcaaaatgtg tctttataga gtcgtcatct gtattttcga ttccagaact
     1501 gtctgataag attactttcc ggatcacgaa tgaaatccag tatgcaacta ctgggagtca
     1561 tttatgctgt ttttcctctt ctttaggtat tatttatttt gacaagatgc cggtattacg
     1621 taatcaagtt tctcttgact tattgcatca tcttttagag ttttgcttag gttcatctaa
     1681 tgtaaggctg gctactttaa aaagaattcg cactggtgat ataatcatag ttcagaaact
     1741 ttataattta ttattgtgta atcaagttat tattggggat tatattgtga atgataataa
     1801 tgaggcaaaa attaatctgt cagaaagtaa tggtgagtca gaacacacag aagtttcttt
     1861 ggcattattc aattatgatg atatcaatgt aaaagtggac tttattcttt tagaaaaaaa
     1921 tatgacaatc aatgaactaa aaatgtatgt agaaaacgaa ttattcaagt ttcccgatga
     1981 catagttaaa catgtaaata ttaaagtaaa tggttctttg gttgggcatg gggaacttgt
     2041 ttctattgag gatggttatg gtatcgagat tagttcttgg atggtaaagg agtaatctca
     2101 tgctgagtga catgtccctc atcgctacac tttctttttt tacgctatta ccatttttag
     2161 ttgctgctgg tacctgttat ataaagtttt ctattgtttt tgtaatggta cgaaatgcac
     2221 ttggtttgca gcaggttcca tcaaatatga ctctcaatgg tattgcattg attatggctt
     2281 tgttcgtgat gaaacccatt attgaagctg gttatgaaaa ttatctaaat ggccctcaaa
     2341 aatttgatac aatatctgat attgtccgtt tttctgattc tggcttgatg gaatataagc
     2401 aatacttgaa aaagcataca gatcttgagt tagcacgttt tttccaaagg tctgaagaag
     2461 aaaatgctga tttaaagagt gcagagaata atgattattc attgttttcc ctcttgcctg
     2521 catatgcgct aagtgaaatc aaagatgcgt tcaaaatagg tttttattta tatttgccat
     2581 ttgttgttgt tgatcttgtt atttcaagca ttttacttgc acttggtatg atgatgatga
     2641 gtccaatcac aatatcagta ccaataaaac ttgtattatt tgttgcgctt gatgggtggg
     2701 gtatcctgtc taaggccttg attgaacaat atatcaatgt tcctgcttag atgatggagg
     2761 taatgtgtct gatatagttt atatgggtaa taaggctctt tatttaatcc ttatcttttc
     2821 cttatggcca gtaggtatag ctacggttat tggattatct attggtttgt tacagacagt
     2881 gactcaactc caagagcaga cacttccttt tggtataaag cttataggtg tctcaatatc
     2941 tttgctactt ctttctggat ggtatggtga ggttttattg tctttttgtc atgaaataat
     3001 gtttttaatt aagagtgggg tttgatggac atttcaagct ggttcgaaag tattcatgtg
     3061 tttttaatac tcctgaacgg cgtttttttt agattggctc cattattttt ctttcttcca
     3121 tttttaaata acggtataat ttctccatct attagaatac ctgtgatttt tcttgttgca
     3181 tcaggattaa ttacttctgg taaggtagac ataggttctt ctgtttttga acatgtttat
     3241 ttccttatgt tcaaggaaat aattgttggc ctccttctct ctttttgctt gtctcttccc
     3301 ttttggatat ttcatgctgt tggtagcatt attgacaacc agcgtggggc aacgcttagt
     3361 agttcaattg atcctgccaa tggtgttgat acgtctgagt tggcaaaatt tttcaatctt
     3421 ttttctgcag ttgtatttct atacagtggt ggtatggtct ttattttaga atccatacaa
     3481 ttgtcttata atatatgccc gttattttct caatgttctt tccgtgtctc aaatatctta
     3541 acatttctga ctttattggc aagtcaggct gttattttag ccagtcctgt tatgatagta
     3601 ttgttactat cagaagtatt acttggtgta ttatcgagat ttgctccgca gatgaatgct
     3661 ttttccgtat cattaactat taaaagttta cttgcaatat ttattatttt catctgttct
     3721 tctactattt acttttctaa agttcaattt ttcctcggtg aacataaatt tttcacaaat
     3781 ctatttgtta gataaaatat tatggcaaat aaaacagaaa agccgacacc taaaaaacta
     3841 aaggatgccg caaaaaaagg acagtcattt aaatttaagg atttaacgac tgttgttatt
     3901 attctggtag ggacatttac tataatatca ttcttttcct taagtgatgt aatgctctta
     3961 tacagatatg taataattaa tgacttcgaa attaatgagg gtaaatactt ttttgcagtg
     4021 gttattgtct tttttaagat aattggcttc ccactttttt tctgtgttct ttcggctgtg
     4081 ttgccaacat tggttcaaac aaagtttgtt cttgcgacta aagctatcaa gattgatttt
     4141 tcagtattaa accctgttaa agggttaaaa aaaatattta gtataaagac aataaaagaa
     4201 tttttcaaaa gcattctgct tcttattatt ctagcattaa caacctattt cttttggatt
     4261 aatgaccgaa aaataatttt ttctcaggtg ttttctagtg ttgatggctt atatcttatt
     4321 tggggggggc tgtttaagga tataatatta tttttcttgg cattttctat ttttgttatt
     4381 attcttgact ttgtgattga gttcatttta tacatgaaag atatgatgat ggataaacag
     4441 gagataaaaa gagaatatat agagcaagag ggacactttg agacaaagtc gagaaggcgt
     4501 gagttgcata tcgagattct ttcagagcag actaaatctg atatacgtaa ttcaaaatta
     4561 gtggtaatga acccgactca tattgcaatt ggtatttatt ttaatccaga aatagcgcct
     4621 gcacctttta tttctctcat tgaaactaac cagtgtgcct tggctgtcag aaaatatgca
     4681 aatgaagttg gtataccaac tgtgcgtgat gtgaaattag ctagaaaact atataaaaca
     4741 catacaaaat atagttttgt tgattttgaa cacttggatg aagtcctacg tcttattgtt
     4801 tggcttgagc aggttgaaaa cactcattaa taagtaaagg agatgtgtta tatgggagtt
     4861 aatttctgta ataaaatagg tattgatcag tctgaatttg aaatagaatc ttccatcatt
     4921 aactccattg ctaatgaggt attgaaccca atatctttcc ttagcaataa ggatataata
     4981 aatgttttac tcagaaagat ttcatctgaa tgtgacctgg ttagaaaaga catttatcgt
     5041 tgtgctctgg agttggtcgt tgaaaaaaca cctgatgatt tataatcatt cataataact
     5101 cttttgctga tataaatatt tttgttgaag atatttatgt agtcacagga tcaattgtag
     5161 aaaattgtta tgtttttgtt aggaactgca ctaaataata tatgataaga cagcaaaaac
     5221 gactgaccat aatccttctt ttattaggag ttgataagag agactattca tcctgtaatg
     5281 ttaaaactct gttatatagt attcgcgatt atgcaaagtc tgtaaatgat catgagatac
     5341 taaccgagag taatcgttta ttatctcatt gcatttcaga tagtaacgga gctttcttta
     5401 aaagtagtaa atacgttccc cttaagtact tacgtaaaag gagaatagca cgaaagatac
     5461 caaatgatta gcactttgct agtaagcttg ctggtaaact atattgaccg ttgggtttgt
     5521 tttgagactg taatttaaat tgtgtaattg cctgtttttg atatgtttac tccaacaacg
     5581 gagacaggta aattatggac gaaaagaagc ttaaagccct tgcggctgaa ctggctaaag
     5641 gcctcaaaac tgaggccgac ctcaatcagt tttcccgtat gctgacgaaa tatcccggct
     5701 cttaaaacga tagtggctca ggctttcatt tttcagcgtt ccccagaag
//
LOCUS       DAJTYZ010000199         5714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000199 DAJTYZ010000000
VERSION     DAJTYZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.199"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(21..1958)
                     /locus_tag="OO596_003876"
     CDS             complement(21..1958)
                     /locus_tag="OO596_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6719608.1"
                     /translation="MNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSRSGI
                     LALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYD
                     TLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLI
                     HEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHH
                     GALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFRLAI
                     IQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDP
                     GIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFA
                     ALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDYPTS
                     VLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVPATI
                     LAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAEVLP
                     SVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAHSAD
                     MTFRVKPESLILTIFFLGLIP"
     gene            2369..3076
                     /locus_tag="OO596_003877"
     CDS             2369..3076
                     /locus_tag="OO596_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6719609.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3182..3257
                     /locus_tag="OO596_003878"
     tRNA            3182..3257
                     /locus_tag="OO596_003878"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3215..3217,aa:Phe,seq:gaa)
     gene            3455..4426
                     /locus_tag="OO596_003879"
                     /pseudo
     CDS             3455..4426
                     /locus_tag="OO596_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            4564..5583
                     /locus_tag="OO596_003880"
     CDS             4564..5583
                     /locus_tag="OO596_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR6719610.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT         1526 a         1451 c         1419 g         1318 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc
      121 actgacggga aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg
      181 acatacagat cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc
      241 ggatatttgt tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc
      301 tgttcaaatt gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga
      361 gttaataaga acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc
      421 agatagtgcg ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca
      481 ttgatacctg gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca
      541 tatccttcaa agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg
      601 gctaacacag atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac
      661 gggcggaaca gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg
      721 cactcagccc acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt
      781 gcagcaaaca gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac
      841 cgcttatgcg ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc
      901 gacgtctgtg agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga
      961 tcgttttcgt taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt
     1021 tcgtaaccga cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg
     1081 atcacctgac gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg
     1141 cgaaacgggc gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga
     1201 tactcttcat taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa
     1261 gcttccagat agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta
     1321 tggttgttac ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact
     1381 ttattcgctg ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg
     1441 gctgcgaatt tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt
     1501 acgtcagcgt tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg
     1561 cgtccggcag gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg
     1621 ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct
     1681 tcatactgac aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc
     1741 gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga
     1801 aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg
     1861 actgccgcga catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa
     1921 agtcgggata ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc
     1981 gtttcgggga agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc
     2041 aaactggtca ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt
     2101 tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg
     2161 gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg
     2221 caaactccgt acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc
     2281 aagccaggac tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa
     2341 tcaaacaata cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc
     2401 agtcttactg ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg
     2461 cgtctccatg acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt
     2521 gaaatgcgcc aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga
     2581 aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg
     2641 tcactcacgt aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt
     2701 cctgttttgc gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga
     2761 tccgagtatt ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg
     2821 ctcgctgggt attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct
     2881 agcgtgggct gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag
     2941 cgccgtaggc ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt
     3001 cccggtggtc aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac
     3061 cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct
     3121 gcagtaaatt gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt
     3181 tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat
     3241 tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc
     3301 agaacgccac gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc
     3361 cccccacatc aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt
     3421 tgtaccctct ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg
     3481 ggctgcaaag cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct
     3541 ggtacatcct aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa
     3601 gactctggca cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga
     3661 tgaggcccga aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa
     3721 agtagtccct gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa
     3781 taaacaatgg gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt
     3841 cccctttatc ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt
     3901 tcgtgctgca gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc
     3961 tgccgtaatg cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat
     4021 ggctggcgca ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct
     4081 gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt
     4141 tatgctgaat ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga
     4201 aattgatatt gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt
     4261 aaaattctca caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca
     4321 ggctgtagcc atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac
     4381 gggagcacat gacccgcgta aaccaatcag tgaaaatgta atgaacgcat cccacacaat
     4441 taaacacgga gcagtatgat ggttaaaagg gtgaaactgg caggtgtgga gcctcgcaga
     4501 tttccggcat catagtgccc aatacgggat gcataaagct ccctcagact ggagactcca
     4561 caaatgaaat atacaccggt tggcgttgat atcgcaaaac atgtcattca gattcacttc
     4621 atcaatgagc acacaggtga agtggttgat aaacagttgc gtagacagga ttttctgacg
     4681 ttcttcggca accgtgagcc atgcctgatt ggtatggagg cctgtggagg ttctcagcac
     4741 tgggcacggg aactgacaaa acttggtcat aaagtccggt tgttgcaggc ccgcttcgtt
     4801 aaggcattcg tcatgggcaa taagaatgat gtgatggatg cccgggctat ctggatggcg
     4861 gttcagcagc cgggtaaaga aatcgccgta aaaacagaag aacagcagtc ggtactggtt
     4921 ctgcaccgta cccgcatgca actggtgaag ttccggaccg cacaaattaa tgccctgcac
     4981 gggacgttac tggagtttgg tgaaaccatc cacaaaggcc gggcagcgat ggagcgggag
     5041 ttccccgaag cactggaacg gatgaaagag agactgccac cgtatctcat tatggttctg
     5101 gaaaaccagt acaaccgact gaatgagctg gactcactga tagaggatat tgaaaaacag
     5161 cttaccagcg tggcgaggca gaatgaaacc tgtaagcggt tgctggatat tcctggcgtt
     5221 ggaccactta ttgcgacggc agcggtggcc accatggggg aagcatcagc gtttaaatcg
     5281 gggcgagagt tcgccgcata tgttggtctg gttccaaaac aaactggctc cggagggaaa
     5341 gtacgtctgc tggggataag caaacgtggt gacacttatc tcaggacatt atttatccac
     5401 ggtgcaagag cggtggcatt agtagctaaa gagcctggcc cgtggataac cgaactgaaa
     5461 aaacgtcgtc cagccagtgt ggcaatcgtc gccatggcaa acaagctggc acgaacagta
     5521 tgggcgataa ccgcccatga ccgtaagtat gacaggaacc acgtcagtat cagaccatat
     5581 taatcgctga taccattaaa caatgaactc ttaacaaaag ggtgaatgct gaaaggttgc
     5641 tatggcggcc agagtgatga caaagacagg taagaccgtg actcactaaa cctgaacagt
     5701 attttgggct tgaa
//
LOCUS       DAJTYZ010000200         5683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000200 DAJTYZ010000000
VERSION     DAJTYZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.200"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            525..1651
                     /gene="ltrA"
                     /locus_tag="OO596_003881"
                     /pseudo
     CDS             525..1651
                     /gene="ltrA"
                     /locus_tag="OO596_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1734..1913
                     /locus_tag="OO596_003882"
                     /pseudo
     CDS             1734..1913
                     /locus_tag="OO596_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1943..2425)
                     /locus_tag="OO596_003883"
                     /pseudo
     CDS             complement(1943..2425)
                     /locus_tag="OO596_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2681..3661)
                     /locus_tag="OO596_003884"
     CDS             complement(2681..3661)
                     /locus_tag="OO596_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6719611.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIHLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3661..4860)
                     /locus_tag="OO596_003885"
     CDS             complement(3661..4860)
                     /locus_tag="OO596_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6719612.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5182..5535)
                     /locus_tag="OO596_003886"
                     /pseudo
     CDS             complement(5182..5535)
                     /locus_tag="OO596_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1569 a         1296 c         1332 g         1486 t
ORIGIN      
        1 agggtttaca ggagcactga ccaaacgacc cggtgctgct tcattctaag gaatgaggtg
       61 gtgagcgact cgggaaaagg cagagatcta ctgtcacgtg gggttcggga agctgaacga
      121 aagtaaactg tccgatgaag catcgaaatc ttagatgatg tcaaaatggg gccgtacagc
      181 cgccccctga taagcgcaga agacacctgc ttactgactg cgcggcatcc ggtgtgaagg
      241 cagcaggatc ccgggccaga ccatctcagg gaacgtgaga acctgtttcc ggcacgtatc
      301 gggaaacagg ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg gtctggagcg
      361 aagggatggg gatcacattc attgcagaag acacaaccca aaaggggatg acgtaactga
      421 tgcaacgaaa atcatttgaa ataccaaaag cactggtctg ggcatcatat ctggatgtgc
      481 gtcgcaacaa gggagccccc ggatgcgacg ggcagacttt gaaaatgttc gaccagcaac
      541 gtgatggcaa tctatacaag atctggaacc ggctatgttc gggaacatgg tttccgccac
      601 cagtgctgga aaaacggatc ccgaagtcga atggcaagga acgcattctg gggatcccaa
      661 cagtatccga tcgaattgct cagggagcga taaaactttt catggaagaa aagcttgacc
      721 cgattttcca tgctgattca tatggttacc gaccaggcaa atcagctcat gacgctctga
      781 aacaatgtgc catccggtgc tggcgttata gctggatact ggaagtggac atcagtgcgt
      841 ttttcgacca tgtgagacat gatctggtgc tcaaagctct ggaacaccac gggatgccta
      901 aatgggtcat cctgtactgt cgacgctgga tggaagcgcc aatgcagagt tgtgaaaatg
      961 gagaattaat aacccgaaca cgaggcactc cgcagggcgg ggtcattagt ccgctacttg
     1021 ctaacttgtt ccatcactat gcatttgatt tgtggatgga aagagaatat cgggggtacc
     1081 gtttgagagg tacgctgacg atattgtagt acattgttca cgaatgagtg atgcaacaag
     1141 gctgaagaac aggttatcgg agcgcttctc ggaagtcggg ctggtcctga atgcagggaa
     1201 aacgaatatc gcctacattg acacgtttaa aaggagaaac gtcgcaacga gtttcacctt
     1261 tctcggatat gacttcaaag tgcgtacgct gaagaatttc aaaggcgaac ggtaccgaaa
     1321 atgcatgccg ggtgcgtcaa atgcagcaat gcgcaaaata acagaaacaa tcaagaagtg
     1381 gcgtatacat cgctcaacag ctgagagttt gctggatttt gcgaggcgct acaatgcgat
     1441 agtgagaggc tggatcgagt actacggaaa gttctggtcc agaaatttca actatcgact
     1501 gtggagtgca atgcagtcac gtctgctcaa gtggatgcag tctaaataca gactttcgaa
     1561 ccggaaggct cagcgaaagc tgacgctggt aaggaaggag tatccgaagc tatttgttca
     1621 ctggtattta ctacgtgcat caaatgagtg atcaagagcc gtatgacgcg agagtgtcac
     1681 gtacggttcc gtgagcagcc ggggagtgaa attctctccg gctgactcga ctcgcgggtt
     1741 ccgacagcga tgacgaacat gcggcggtgt tgtactcgct gatcggcaca tgccgtctga
     1801 acaatgtgga accagaaaaa tggctgcgtt acgtcattga gcatatccag gactggctgg
     1861 caaaccgggt acgtgatctg ttgccctgga aagttgatct gagctctcag taaatatcaa
     1921 tacggttctg acgagccgct tacttttggc gtaaatctgc tgcgccttta ctgaacacaa
     1981 tgccgtattc agcaagaagt ccacgaatgt gatttatcag ttgtgtcctt tccattgaaa
     2041 ccaaccgacg catattcaaa aaagtctgga cgtcctgttg ctccagagtt tttattggta
     2101 cgggtcgtat agttccatgt tggcaggcct cagctattgc ctgtgcgtcg ttataatcat
     2161 ttttctggca ccgctgataa gctttaacat gttgtgccgg aagcaatatt acatcatggt
     2221 cgagtttcct gatttcccgg gcccagtaat gagatgcccc gccagcctcc atcgcaacag
     2281 tcattttagg ctgttgcacc agccagttaa gcagttgatt gcgagaaagt ttggtctttc
     2341 ctgcgggttt gccatgagca ttgatactga aaagataaaa gttcgtttta gccagatcaa
     2401 tgccggtgat tttctgttca gacatggtat tccgcttact tttgtggtta aagtttatcc
     2461 atcatggcgc aattgaaggc tgccgtgaaa gagggggaag actatctcat cggttactgt
     2521 taaacggcta agagaaacta tctttttcaa gcaaagaaga tacggtgaaa caggcgattt
     2581 caccgtgaaa tactgttcaa aaaaaacgct aataaaagca aaatttcttt taaaacagcc
     2641 aattaaaaac tggtgagttg ctggcgaaaa acttgaattt tcacagcgaa ttcagattat
     2701 cttttagtac aagagcaatc atacgatcta acttatcctg aacttccaac ggtaatctgc
     2761 cgaattcata tgagaacgta cgtcccttta ctcgttttcg agcaaattta tctttactat
     2821 caaatttcca taacaaagtt acaaccgctt tatcctttac tcctgaatca agcaatgaag
     2881 acatttcctt ggttataagc cgcataatac tgtttttaac ctcgtcttca gaaagattgt
     2941 catcagcagt aataagctca atactcggcg agatattctt cacaacttca tcaatagata
     3001 tatcacccag cctcagtcgt tcacttaatt cagccagttg cctgtaatcg gcgtatgtca
     3061 gctcagactg aacaggaaaa agagacacaa aatctttagg tacggacgct gcttggaggg
     3121 cacgagtcac cttagctgcc gacaatccct ctctttccgc aatttgcttc tggttcatac
     3181 cggcctcttt gagacgtact aaacgcaacc caatttcacg aatattatgt tcaagtgacg
     3241 tctgcaaatc tttcgccaga tgctgagctt cagaaacagt aagctcatca tccgttacaa
     3301 gtacacgtaa tccaaccttg catagcaaag ctgctgcccg acgacgggaa ccatccaaaa
     3361 tctcaataag atcgccagtc cgaatcccaa tacatggata gaattgttgt aaatgtatag
     3421 ttctggtgat gtcttttagt gagtccggcg tcagggctgt ctgttcgcgg ccattcacct
     3481 cttcaaccac aaaagtttga gtgtccactt tgtcatgatc aaccttgatc tcagtaaatt
     3541 ttgcctttcg gccagtattg agggtaaata catgaactgg agccgatatt tcttcagtat
     3601 tattcaggat atttgtattc agcgtacggc caatagtcgg tcggtgcttt ctgttttcca
     3661 tcagtagttg gcccttataa attcaatacg atcaaaaact gctttagcga aatcttctgc
     3721 tgccatacgt gcatttttca aagcttcacc actgccaaca taagtaacag gattcgcaga
     3781 tatcacggta tcaaacgact cgccgcttct ctcaaaacca tcaagacgtg gcattgaaac
     3841 atcaagcatg tcaccaccga aaatctcttt tgtcaggcta tggcagtatt tatgatctga
     3901 cttattcgcc agtttcgaca tgaagccaat atttgcctgc aaacggcatg aacatccatc
     3961 ctgttcaata atctgtacaa gttcaggtaa acgagccaaa tatttaagcg tggaatggaa
     4021 atcaacttgt gctggcggca ccggtgtaaa catgatatct gcggcagcaa tggcattttt
     4081 caaaaatgcg tccagatgcg ggcctgtatc aataaggatg aagtcgaaat catgcttcag
     4141 cttatcaata atattctcac gcaatatcgc gtgtttgttt tgtcctaata gatgctcctc
     4201 gcacagagta tcccagtttg atgcaataaa ggcgtcgtca atagaagccg gcataacata
     4261 aacgccaggg ataactgacg gaacgataaa atcttccagt agttcctcac gactcacgtt
     4321 ctgcaacata gcctgcggtg cggttgtatc aaccagcccc accgcatgca agtagttcag
     4381 aaacatagtg gctgatgact ggggatctaa atcaaggagc agaatacgta aatcttctga
     4441 cagcaggtga ggatgagcac gcagggcgtg agctacggaa acactactca cggttttaga
     4501 cacaccgcct tttagactac cgatgaatat ggaatatgcc tcactataac gatcgcgata
     4561 tttaggaatg ccacgatgtg catagatatc gataatgttc tggatggtta atgcatattt
     4621 tgtcgcggtt cctgctgggc gtttttcaaa ctgatagccc tgagcctcca tctcaccgac
     4681 tgcaccatcc acgcttgcgc gcgttaattt tggaagctta gctacagcag actttgaata
     4741 gacttgataa aaaacatcag cctgaaactc ctgcttttgc tcctgaatct gttttgttag
     4801 ggcgagcaac attttatctg cacgctgcgc aaccttactt aactgctcaa aactagtcat
     4861 tccccctccg tgctatgtag gattttaact tattacaaaa aaaactgcat tagcagggta
     4921 atgcatgcaa gtacatattg tacatgctta tattaaaaat gcgcgcattg ctgatttatt
     4981 aatcatcgga atgaatatta ctgtaagata gtatgccttt ccgggagaaa gacgctgatt
     5041 tcccccgaat aatccagttc accaccacac tccggacaac agctttcttc aggctccaga
     5101 tggtgtattt cgcgggaaac atgttccggt aacggacggc gatgacgtga ctgtcgcaac
     5161 aggcgggggt agtgcttcca atcagtagaa catgtgcttt tcaatgaagg agccgatgac
     5221 tttttcgtgg atttccacgg aacgcgagaa gcagattgtt ttacgtgcca gccgcttgat
     5281 gcgggtgcgg agggtccggt tgttacgttc aatgcgttgc gtgaagattt tgccggtcag
     5341 gtgtttctcc ttcggcactt ctctggcgta gctgccccag tcatcgctgg tcaccatgcc
     5401 accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc
     5461 cgtggcccga aagtcgaaag tgtaagccaa cacaccgcca gttttagtgt tgtacgcata
     5521 ccagagccag tgttgtgtca acgacggatg aaaagtgatc cacttatatc tccaccaacg
     5581 gcccaatatt gatccaccgt tttactcagg attagcttct gctataaccc cggcctttcg
     5641 tttctgtctg agtcgatagc tttctccttt gatttgaacg aaa
//
LOCUS       DAJTYZ010000201         5542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000201 DAJTYZ010000000
VERSION     DAJTYZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.201"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO596_003887"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO596_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6719613.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO596_003888"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO596_003888"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6719614.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO596_003889"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO596_003889"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6719615.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO596_003890"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO596_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6719616.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1371 a         1327 c         1507 g         1337 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aa
//
LOCUS       DAJTYZ010000202         5478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000202 DAJTYZ010000000
VERSION     DAJTYZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.202"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(279..650)
                     /locus_tag="OO596_003891"
     CDS             complement(279..650)
                     /locus_tag="OO596_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719617.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(711..1208)
                     /locus_tag="OO596_003892"
     CDS             complement(711..1208)
                     /locus_tag="OO596_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719618.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1284..2072)
                     /gene="aadA1"
                     /locus_tag="OO596_003893"
     CDS             complement(1284..2072)
                     /gene="aadA1"
                     /locus_tag="OO596_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6719619.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2130..2654)
                     /gene="sat2"
                     /locus_tag="OO596_003894"
     CDS             complement(2130..2654)
                     /gene="sat2"
                     /locus_tag="OO596_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6719620.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(2749..3222)
                     /locus_tag="OO596_003895"
     CDS             complement(2749..3222)
                     /locus_tag="OO596_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6719621.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3554..4090
                     /locus_tag="OO596_003896"
     CDS             3554..4090
                     /locus_tag="OO596_003896"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6719622.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            4205..4531
                     /locus_tag="OO596_003897"
     CDS             4205..4531
                     /locus_tag="OO596_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6719623.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(4719..4958)
                     /locus_tag="OO596_003898"
     CDS             complement(4719..4958)
                     /locus_tag="OO596_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6719624.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1426 a         1306 c         1219 g         1527 t
ORIGIN      
        1 tctctctaga tagcagggat ttcttggcct tgcctcatgc tgatagcttg taaatatcac
       61 acgatcctta gccccacaaa gtgagcatac tccgtagctt tcatttatag aaatcgtcgt
      121 tagattgtcg cagcatgtgc acgttccgac tatttgctca ggaagcatcg ccataccttg
      181 gcctaggtaa agaagatcag actcgtagcc acatcggtgg caactgagct tgtacgcaat
      241 gcccatctgg ttgtctcacc tgaatcgtaa cgtcagagct attcatccat ccattcggca
      301 tcaatgtatg ccactaagcc gaccagctga ccgtttggac tgaagtatgt tgcctgcacc
      361 ccacttgaca tctcatcaat accaccgcac tgcgctaggc gccggtccca cttgcccaca
      421 gaagcgtcag gatcggctga gttccccaag acccttgtga gccatgcctc ctttaactca
      481 acctctcgaa tgattgcgtc ggccagctcc tccgcgttat ctagttcgac atagtctgga
      541 attcggacgt acgttctgaa cacagctgga tacttacttg ggcgattgtc atacatgaca
      601 tcaacaatgt acccgtttgt gtaaccgtct cttggaggtt cgtatgacac tagtggttcc
      661 cctcagcttg cgactagatg ttgaggccta acattttatt agagagcagg ctagttgctt
      721 agatacatga tcttcaggcc gttatctgtc agggcaagcg aaaattggcc atttatgacg
      781 accaatgccc cgcagaagct cccatctttg ccgccataga cgccgcgccc cccttttggg
      841 gtgtagaaca tccttttgcc agatgtggaa aagaagttcg ttgtcccatt gttggcaatg
      901 acgtagtagc cggcgaaagt gcgagaccca tttgcgctat atataagcct acgatttccg
      961 ttgcgactat tgtcgtaatt ggatgaacta ttatcgtagt tgctctcaga gttgtcgtaa
     1021 tttgatggac tattgtcgta attgcttatg gagttgtcgt agttgcttgg agaaatgtcg
     1081 tagttggatg gggagtagtc atagggaaga cgagcttcat ccactaaaac aattggcagg
     1141 tcagcaagtg cctgccccga tgccatcgca agtacgaggc ttagaaccac cttcaacaga
     1201 tcgcgcatag tcttccccag ctctctaacg cttgagttaa gccgcgccgc gaagcggcgt
     1261 cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc gcctttcacg
     1321 tagtgaacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttgtccaaga
     1381 taagcctgcc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
     1441 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
     1501 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
     1561 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
     1621 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
     1681 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
     1741 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
     1801 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
     1861 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
     1921 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
     1981 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
     2041 gtcgatactt cggcgatcac cgcttccctc atgatgttta actcctgaat taagccgcgc
     2101 cgcgaagcgg tgtcggcttg aatgaattgt taggcgtcat cctgtgctcc cgagaaccag
     2161 taccagtaca tcgctgtttc gttcgagact tgaggtctag ttttatacgt gaacaggtca
     2221 atgccgccga gagtaaagcc acattttgcg tacaaattgc aggcaggtac attgttcgtt
     2281 tgtgtctcta atcgtatgcc aaggagctgt ctgcttagtg cccacttttt cgcaaattcg
     2341 atgagactgt gcgcgactcc tttgcctcgg tgcgtgtgcg acacaacaat gtgttcgata
     2401 gaggctagat cgttccatgt tgagttgagt tcaatcttcc cgacaagctc ttggtcgatg
     2461 aatgcgccat agcaagcaga gtcttcatca gagtcatcat ccgagatgta atccttccgg
     2521 taggggctca cacttctggt agatagttca aagccttggt cggataggtg cacatcgaac
     2581 acttcacgaa caatgaaatg gttctcagca tccaatgttt ccgccacctg ctcagggatc
     2641 accgaaatct tcatatgacg cctaacgcct ggcacagcgg atcgcaaacc tggcgcggct
     2701 tttggtacaa aaggcgtgac aggtttgcga atccgttgct gccacttgtt aacccttttg
     2761 ccagatttgg taactataat ttatgttaga ggcgaagtct tgggtaaaaa ctggcctaaa
     2821 attgctgggg atttcaggaa agtaaacatc accttccggc tcgatgtcta ttgtagatat
     2881 atgtagtgta tctacttgat cgatcaggct tttgtatatc tccccaccac ctgaaacaat
     2941 gacatgatcc gttattttct ttaggttggt taaagcatct ttaattgatg gaaagatcaa
     3001 tacgttctca ttgtcagatg taaaacttga acgtgttacg accgcatact ttcggttggg
     3061 taatgctccc attgattcaa aagtcttgcg tccaaccaac agccattggt tataggtaat
     3121 agctttaaac aggagctgtt cacctttggc actccatgga atatcagggc cattcccgat
     3181 aactccattc ttcgatatag ctaccattag tgatagtttc acaattcttc ctcagaggtt
     3241 aacattttat tagtaagcat gctcgttttg tcacccgctg atgcttaccg ttaattaata
     3301 taatcacttg tattaaatag actttcttta aaaatacaag acactctgtt attacaaatc
     3361 gtgcatgccg tctatcctgc ttgcacgatg cacattatct cacccaaaga actcataaca
     3421 ttttaattta tttagcatta aattaaaact ctaccggtaa acaagcatct ctaggcgtaa
     3481 aaaacaggca cttttttaaa tcccagctag aataggctgt ataggcagac agttgcaaga
     3541 caaggagcgt tttatgtcta acagtccatt tttaaattct atacgcacgg atatgcgaca
     3601 aaaaggttat gcgctgaaaa ctgaaaaaac ttacctgcac tggattaagc gttttattct
     3661 gtttcacaaa aaacgtcatc ctcagaccat gggcagtgaa gaggtcaggc tgtttttatc
     3721 cagcttagca aacagcagac atgtagccat aaacacgcag aaaatcgctt taaatgccct
     3781 agcttttttg tacaacaggt ttttacaaca gccgttgggc gatattgatt atatccctgc
     3841 aagcaagcct agacggctac cctctgttat ctctgcaaat gaagtgcaac gcattttgca
     3901 ggttatggat actcgcaacc aagttatttt tacgctgctg tatggtgcag gtttgcgcat
     3961 taatgaatgc ttgcgtttgc gggttaaaga ttttgatttt gataatggct gcatcactgt
     4021 gcatgacggt aagggtggga aaagcagaaa cagcctactg cccacgcgcc taatcccagc
     4081 aataaaataa ctcattgagc aagcgcggct tattcagcaa gacgacaact tacaaggcgt
     4141 agggccatcg ctgccttttg ctttagatca caaataccct tctgcttatc gacaagcggc
     4201 gtggatgttt gtctttccct ccagcacgct ctgcaaccac ccgtataacg gcaaattatg
     4261 ccgccatcat ctgcatgact ccgttgcgcg aaaggcattg aaggcagccg tacaaaaagc
     4321 aggcatcgtt agcaagcgtg tcacttgtca tacatttcgt cactcgtttg ctacgcatct
     4381 attacaagcg gggcgtgata ttcgcactgt gcaagaactc ttagggcata acgatgttaa
     4441 gaccacgcaa atctatacgc atgtgttggg tcagcatttt gccggcacca ccagtcctgc
     4501 ggatggactg atgctactta tcaatcagta aaagtagata aatatcttta tgtagccctg
     4561 agtagcccct gatacttttt atggtgaaaa agataaacaa tcatagaggg gataaatatg
     4621 agccagttct accattgttc agataagttt aagcaagata ctgtcaatca cagtgaccat
     4681 tcatggtcat tacacttcct ccagttgcca gacaatgcct aacataaaaa cgcaaaagcc
     4741 caaaagtcag agcttaggtg ttctaggcgt tttctaagcg ctgaagaaat acgtcttacc
     4801 gtagattgcg tgcgtttctg aagcacattt tccttataca tggctttatc ccaggcagct
     4861 tcatcaagct cttggagtaa caagccagta ataatgcgac actctcgggt ttgtagcgag
     4921 ccgctggtga gatcggagtt atagcggaaa ggggtcattt gtaattattt gtgggtggag
     4981 gatagttaac ttattattgt agttggaaga ggctgctttc caaacattag cacctcgtaa
     5041 tgcttgttac tgtatattaa tttatgctct tagcgcatgt taatgggcta ttacaatgca
     5101 taatgtccac atcaagataa ctgtggctta acacctgtac aagcgaaaaa aatcaatcac
     5161 ctgtccggaa atacgggatc actgtaaagt gttggtgagg cagtctagga gggggattta
     5221 atttgggata ctgatgaaag gattatgatc agttggatat aggatcgtac taagctgagc
     5281 ggcaggttag ctagattagt taaccagata agtgaaatct agttccaaac tattttgtca
     5341 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     5401 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     5461 gcccacaagg gctgccga
//
LOCUS       DAJTYZ010000203         5462 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000203 DAJTYZ010000000
VERSION     DAJTYZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.203"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..115)
                     /locus_tag="OO596_003899"
     CDS             complement(<1..115)
                     /locus_tag="OO596_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6719625.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="OO596_003900"
     CDS             complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="OO596_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6719626.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2333..2968)
                     /gene="nth"
                     /locus_tag="OO596_003901"
     CDS             complement(2333..2968)
                     /gene="nth"
                     /locus_tag="OO596_003901"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6719627.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="OO596_003902"
     CDS             complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="OO596_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6719628.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="OO596_003903"
     CDS             complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="OO596_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6719629.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="OO596_003904"
     CDS             complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="OO596_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6719630.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5350..>5462)
                     /locus_tag="OO596_003905"
     CDS             complement(5350..>5462)
                     /locus_tag="OO596_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HCR6719631.1"
                     /translation="NAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         1445 a         1384 c         1340 g         1293 t
ORIGIN      
        1 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
       61 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
      121 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta
      181 acgaaaagtt agcggcttgt aaaagagttt ccctgaattt tacgctacag cagctttcgc
      241 cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat
      301 cagtactgca ataatggcag tagcgacacc aatctgcaag aatacgcgac cataaacttc
      361 cagtgacatc agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc
      421 accaatcagg tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag
      481 acgctgcgga acgagttgtg caaccattgc cagacccaga ccagagatca tcagttcccc
      541 gatgctctgc aggccatagc ttgcgaccag ccagcttaca gacacgatac cagcgtcaga
      601 cgcgaatttc gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat
      661 tgcaaacttg gttggcatcg gcagggtatc gcccatcttg ttatagatag cggccagaat
      721 cggactaccg atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc
      781 cagacccaga atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat
      841 ctggctgtac agcacgaaga agataatggc ttcgagcatc aggatgaagg caacgatcat
      901 tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc
      961 gaaggcaaca acgcccagcg ccatacgcgc aacttcctga ttgtgcagca gccaggtggc
     1021 gatagcgatc agtgccacaa caccaataat ggtcagcagc aggttacggt agttgatagg
     1081 ctcgaagtct ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac
     1141 gatagtgatc agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc
     1201 ggccagccac ggcgtagcaa tcatagagaa gaaagagccg atgttgacgg acatgtagta
     1261 catggtgaat gcaccgtcca gacgcgggtc gtttttctca tagcatgtag aaagcagaga
     1321 agatgggtta gctttaaaca ggccgttacc gaccgcaata gccgccatac ccatataaac
     1381 gataccggcg tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc
     1441 accgagcata attacgcgtt tagtacccag taccttgtca cctaaccagc cgccgatagc
     1501 gaccagacca taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga
     1561 catacccagt tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa
     1621 acgttcccat aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt
     1681 caaactgacg ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac
     1741 atcccatatt aacgggggtg ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt
     1801 atagtgggag gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt
     1861 gtaataatct gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc
     1921 agcgttaaat ttttcaaaaa acgtaatggc agattttttc actgcactga gaggggggtg
     1981 atggcttaac ggcgatatgt tctgctattt agcgttttta ctagtgagat agtccagttt
     2041 ctgaaaaata gccagtgtaa tgttttgtag gtcaatattt gttgcgtgtg taaatttaaa
     2101 ttattttaac ctgattcttg caatgttttt acattgcgac agcatcgtga tcttgatcac
     2161 gaatgaatag aaaatttcgc tattaaaaaa acaattaacc tgcatttttg gttatccgca
     2221 aacggattac ctgatgataa cgctaagcaa tggcatcatt cacgttaaaa agatgtttta
     2281 aaagaggata aagaaaggtt atcaatgggg taatcggtgt tacccctttt cttcagatgt
     2341 caactttctc tttgtattca caaagatctt caataataca agagccacag cggggcttgc
     2401 gggcaatgca ggtataacgc ccgtgcagga tcaaccaatg gtggcagtcg actttaaact
     2461 ctgccggaac cactttcagt agcttttctt ctacctgttc gacgtttttc cccggcgcaa
     2521 attgagtacg attacaaacg cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga
     2581 atgcagtgtt taatacgacg ttggctgttt tacgacctac gccgggcagg gcttcaagcg
     2641 cagcacgatc ttccggaacc tcgccattat gctgctccag taagatacgg caggttttga
     2701 tgatattttc tgctttgctg ttataaagcc caatcgtttt gatataggtt ttaacccctt
     2761 caacgcccag ttcaagcatc gctgcaggcg tattcgccac cgggtaaagt ttcgccgtcg
     2821 ccttattaac actgacatcg gtcgcctgag cggaaagcag tacggcaatc agcaattcaa
     2881 aaggcgaact gaaattaagc tcggtggtgg gatgaggatt gttctcacgc aggcgagtga
     2941 ggatctccag gcgttttgct ttgttcatca gacattccct gtttcaccgt ttggcaatgc
     3001 acgttcggca gctgcttcag tacggcgctt tttcattctt tcatcaatca ggtattttcc
     3061 tgccagcatc agtcccaggc caataaatgc acctggtggc agcatcgcca gcaggaaagg
     3121 ggagtcggtg tggaaaatct ccacgcgtaa tacttttgcc cagctaccta acagcgcatc
     3181 tgcaccgtca aacaatgtgc cattgccgat aatttcgcgt agtgaaccca gcacgaacat
     3241 ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt gccgaaagcg ccggaccttt
     3301 tttggcggcg aaggcttcag cgcggcccac aacgatacag ttagtgacaa tcagcggaat
     3361 aaaaatccct aatgattgat acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc
     3421 gctgaccacc gaggcgatga tcatcacgta aatgggaatg cggatctcgg ctggcgtcca
     3481 gtgacgcagc gtcgaaatgg tcaggttggt cagcgtcagt accagcgtag tcgcaagtcc
     3541 taaacccaga gcgttagtgg cagtggacgt gaccgccaac agaggacaaa ggccgagcaa
     3601 ctggaccagc gcagagttgt ttttccacaa cccctgaaca ataacgtctt taatttcgct
     3661 cacgctttat tctccacagg caggaagttg agaaagttgt gccggtaacg tctgagcgta
     3721 caatccggcg cgttttaccg cattaaccac cgcgcgggga gtaatcgtcg cgccggtgaa
     3781 ctggtcgaaa tcaccaccat ctttcttcac cgcccagtgc gcatcatccg caccactgat
     3841 ttttttaccc gcaaaatggg tgatccagtc agaaaggcgc agttcgattt tatcgccaag
     3901 ccctggcgtt tcgtggtgct ctgtcacgcg cgtgccaagt accgtgccgt taaaatcggc
     3961 tcccaccagc agctgaatcg caccggaata gccatctggc gcggttgctt ccagaacggc
     4021 ggctaccggt ttgtcatcct gtttggcgat gtaaacccga tgctcacctt tacctaactc
     4081 tggcgcagtt accagatagc aactctgtgc cagcgtattg ttatagcgtt cggctggcag
     4141 cacctgatca aataatgcct tttgttgcag actggcctgt tcagcaatcg tcgttttggt
     4201 catctggttg atggccgcag ttaaccctgt tgaacccgct gcaaatagcg ctaacgtaat
     4261 gccgtgtttt cggatagttt tcagcatggt ttaccctttg cgatggccgt agacgcgcgg
     4321 acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc agcaggacgg caaaagccac
     4381 gccgtcagga tagccgccga aactgcggat caaccagact aataagcccg caagcgcgcc
     4441 gaaaataaga cgaccacgat tggtcgtaga agcggtaacc gggtcagtca aaataaagaa
     4501 tgcgccgagc atggtcgcac cagacagcag atgaatttgc ggtgctgcca gtgtttctgg
     4561 tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt actaagaagc tgaggggaat
     4621 atgccagcga atcgctttct gccatagcaa ccacaggccg ccagccagcc aggcgagatt
     4681 tacccattgc caaccagcgc ccgccagaat accgctgtag atcggatatt gcataatctg
     4741 ttcaaccgaa tgaccggcac ggacagaggt tttaaatgta tccagcggtg tcgcctgact
     4801 aatgccatca atacctaagc gtagtgtgtt catatcacca ccactggcgg tatgcccgct
     4861 gaaaataacc tgtatggcgt cgataaaacc agggatgttg accgcaattt catgtggcgg
     4921 taaccagctg gtcatctgca ccgggaagga gatcagtaag accacataac caatcattgc
     4981 cggattaaac gggttttgcc ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa
     5041 caccgtaccc agcacgacca tccaccatgg cgcgaggggg ggaatactta ccgccagcaa
     5101 taagcctgtc agcaatgctg agttatcttt caacgttgcg gctaccgact gcttgcgtag
     5161 tttgagtacg agagcttcgg ctaacagagt actaaccgat gccaacagga tctgaacgag
     5221 agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct ggcacggctg cgagcaacac
     5281 caacagcata atgcgcgatg tctggcgctg gttatgggta taaggggagc tagctattct
     5341 gaataccatt tagtcctcgt ttacaacctt ctgctgggcg gcttttttgg cctgagcgcg
     5401 ggcaatagcc gcggcaactg ccgctttgcg cggatcaacc tgttcttctg gttctgcgtt
     5461 ag
//
LOCUS       DAJTYZ010000204         5387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000204 DAJTYZ010000000
VERSION     DAJTYZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.204"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            200..433
                     /locus_tag="OO596_003906"
     CDS             200..433
                     /locus_tag="OO596_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6719632.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(466..1104)
                     /gene="ompW"
                     /locus_tag="OO596_003907"
     CDS             complement(466..1104)
                     /gene="ompW"
                     /locus_tag="OO596_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6719633.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1344..1433
                     /locus_tag="OO596_003908"
                     /pseudo
     CDS             1344..1433
                     /locus_tag="OO596_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1461..2204
                     /locus_tag="OO596_003909"
     CDS             1461..2204
                     /locus_tag="OO596_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6719634.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2234..2773
                     /locus_tag="OO596_003910"
     CDS             2234..2773
                     /locus_tag="OO596_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6719635.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            2878..3276
                     /gene="yciA"
                     /locus_tag="OO596_003911"
     CDS             2878..3276
                     /gene="yciA"
                     /locus_tag="OO596_003911"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6719636.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3316..4035)
                     /gene="tonB"
                     /locus_tag="OO596_003912"
     CDS             complement(3316..4035)
                     /gene="tonB"
                     /locus_tag="OO596_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6719637.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4126..>5387
                     /locus_tag="OO596_003913"
     CDS             4126..>5387
                     /locus_tag="OO596_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6719638.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRR"
BASE COUNT         1379 a         1257 c         1304 g         1447 t
ORIGIN      
        1 ttcaacacac tgcacaataa aattaatccg tataaggaaa actgatatgg caactttgac
       61 aaaaaaagaa cgggcatggt tgaacgaatt acaggaagtt cttgatcgct gtcctgtggt
      121 gtcatcctgc cgcgattgcg ttcttagtcc gtggctattg caccaccatc acgcgaaagg
      181 gacagctaag cgcggcggga tggttaagcc agcaacatta accgttgcat ttaaaaaagc
      241 ccgggattct gtggattaca actggcgtgc taatggcacc ccaccctctt tccatgagca
      301 gagatcttta tcagagcgat tgttcagaga gcagggggtt gataccaaaa ttttgctagg
      361 ccattcgaat caaaaaatga ccgatattta caacgatgta cgcggtaagg agtggaaaaa
      421 actggtcatt tgataaccag ttttgcagag gttttgcaga gaaaattaaa agcggtaccc
      481 ggcagagaac ataaacaccc acggatccag tcgtaccgag tcttttacgg tagtaacacc
      541 agatttgtac ttagccgtgg tatcgatatc catgtaccac actgacatgt taaccaacca
      601 gtcacggtta atcagataat caacccccac ctgcccggca gctccccagt aatctttcag
      661 actgagatcg gaaagccccg cctctttgcc atgatcgtta aatccattat caaagaaggt
      721 ggtgtagtta atacctgccc caacataagg acggaatttg ctgccggcat caccaaaata
      781 ccactgcgcc atcagtgttg gcggcagatg atgaacggtt gcaatatcgc cggtcgcccg
      841 ggtgccgatt ttatggcgga acggcgtcgc tgccagtaat tccacaccaa tgttgtcggt
      901 cgccatataa gtaaacgtca ggcccagttg cgtgttattg gtcacgctga atccacccag
      961 acttcctaac gtaccaccag caccttctgt tggacgtacg gttgcagaac ctgcacgcat
     1021 aaaaaattcg cctgcttcat gcgcaaaggc actgccagag agaagagttg ttactgccaa
     1081 agccgccact gttaactttt tcatatccgc tccgtcgtta tggttataaa aacgtgacga
     1141 atatacctac attggagtaa caaatgatcc tacatagatc acattacata tgataattca
     1201 ccctttattg atctggatta atttagaagt tgcattgaga atcctggtta acgtgattta
     1261 aatcaatttt tagatatttt cgctacgcaa atattaactc gttcgccact tgccgcggtt
     1321 tgcccacgcc tgaaggccag caattacctc cgctttacaa agatatgctt ttccatacaa
     1381 gacctgatgc tgccacccga ggcttaacca gggtacaatt gcccgctaat taacacctgc
     1441 ataaaactca aggagagtgc atgtctatca cggcgcagtc cgtataccgt gacaccggaa
     1501 atttcttccg taatcaattt atgaccattc tgttggtatc gttgctatgt gcgtttatca
     1561 cagtggtgtt agggcatgtt ttctcaccca gtgatgcaca gcttgcgcag ctcaatgacg
     1621 gcgtgcccgt tagcggcagt agtgggttgt tcgacctggt tcagaatatg tcaccggaac
     1681 aacaacaaat tttgctgcag gcttcagcgg cgtccacttt ttcaggatta atcggtaacg
     1741 ccattctcgc cggaggcgta atattaatta tccagctggt gtctgcgggt cagagagtca
     1801 gtgcgctgcg agctattggt gccagtgcgc cgatattgcc caagttattt attctgattt
     1861 ttctgactac ccttttagta cagattggca tcatgctggt ggtcgttccg ggaattatca
     1921 tggccatttt actggccctg gcaccggtga tgctggtaca ggacaaaatg ggcatttttg
     1981 cctcgatgcg tagcagtatg cggctgactt gggcgaatat gcgtctggtg gcacccgcag
     2041 tactgagctg gttgctggca aaaacactgt tgctgctttt tgcctcttct tttgccgcat
     2101 taaccccgga aattggtgcc gtactggcga acaccttgag caacctgatt tcagccatat
     2161 tgctcatcta tctgttccgc ctgtatatgt tgattcgcca ataaccttaa gcatttgatt
     2221 acggaatcgt aaaatgaagc agtttcttga ttttttaccg ctggttgtct ttttcgcgtt
     2281 ttacaagatt tatgacatct atgcggctac tgcggcgctg atcgtcgcca cggcgattgt
     2341 gcttatctat agctgggttc gctttcgtaa ggttgagaag atggccctga tcacttttgt
     2401 tctggtggtc gtcttcggtg gcttgacgct gttcttccac aatgatgagt ttattaaatg
     2461 gaaggttaca gtcatttatg ctctgtttgc gggagccctg ttagtcagcc aatgggtgat
     2521 gaaaaagccg ctaattcagc ggatgctggg taaagaactc acgctgccgc aaccggtatg
     2581 gtcgaagcta aatctggcct gggctgtttt ctttatcctt tgcggtctgg caaacatcta
     2641 catcgcattc tggctgccgc aaaatatttg ggtcaacttt aaagtctttg gcctgaccgc
     2701 ccttacctta atcttcacat tgttaagcgg tatctatatc taccgccaca tgccgcagga
     2761 agataaatcc taaccagact gccagtcctt cggggctggc gctgtatctc ctctgttctc
     2821 ctcaggaaaa tcatagtagc atcgcgcctg tgattttcct tttaagtcgg ttttaccatg
     2881 tctacaacac ataacgtccc tcagggcgat cttgttttac gtactttagc catgcccgcc
     2941 gataccaatg ccaatggtga catctttggt ggttggttaa tgtcacaaat ggatattggc
     3001 ggcgctattc tggcaaaaga aattgcccac ggtcgcgtag tgactgtgcg ggttgaagga
     3061 atgactttct tacggccggt tgcggtcggc gatgtggtgt gctgctatgc acgctgtgtc
     3121 cagaaaggga cgacatcggt cagcattaat attgaagtgt gggtgaaaaa agtagcgtct
     3181 gaaccaatcg ggcaacgcta taaagcgaca gaagcattat ttaagtatgt cgcggttgat
     3241 cctgaaggaa aacctcgcgc cttacctgtt gagtaatagt caaaagcctc cggtcggagg
     3301 cttttgactt tctacttact gaatttcggt ggtgccgtta attttaaaca ggatattcac
     3361 cacaatccca ctgcctggct tacccggctc ataacgccat ctgcgcatcg catttttcac
     3421 ctcacgctca aacatgttcg caggcttggc tgagaggatt tgtacgttat ccacgcgacc
     3481 atccggcgtg acgtcaaatt taactttaac ctgcccttca atgcgcaatg cctgtgctcg
     3541 tgccggatac tgcggctgat tacggcttaa tgcgcgtggt cctgaagcca cactggtaac
     3601 cggcttgctg gttgcagccg ttgccgtact tgatgtgggg cgcgcgggtg ccgtattttc
     3661 aaacggtgat gccggacgcg actctacggg tttgacatcg cgttttggct gctcctgtac
     3721 ctttttcacc ggcttcggtt ttggcttagg tttcggcttc ggcttttcaa tgaccaccgg
     3781 tgcttctttt ggcggttcgg ggatcggctc aggttccggt tctggctcta ccaccggctc
     3841 cggtggcggc tgaacggctt gtggcggttc gagatcagca ggcgcaacca tcgtgacaga
     3901 aattggctgc gcaggcgcag gtagttcaat aacctgatgt accgaggtat agagcagacc
     3961 cgccacaaca gcaccatgaa tgcagaccga aagtaacgtc ggccagggga agcggcgagg
     4021 taaatcaagg gtcattgtat tgtagctatt cggcattgta gcattagcca taacgtttat
     4081 aagttcatct ttaaatgata atcaattgca tgggagttta tcaccgtgag aaccgtaata
     4141 acatacttga gattctcatc agccatacaa ggcgctgaag gtgcagattc aaccagacga
     4201 caaaacgacc tgttcaagca gtggctgaag aagaatggcg atgctcaaat agtagcgtct
     4261 ttcagtgatg aagggttgag tggttacaaa ggtaaacacc ttactggtca gtttggtgac
     4321 atgttagccc gtattgaggc tggggagttt ccagaaggta caattctgct agttgagagt
     4381 atcgaccgta taggccgatt agagcatctt gaaacagaag ccttgatgaa ccgcatctta
     4441 ggcaacggga tagagattca cacattgcaa gatggcctta tctacacaaa ggatgcttta
     4501 gccgatgacc ttggcataag catcatccag cgtgtcaaag cctacatagc gcatcagaaa
     4561 tctaagcaga agtctttccg tgtttctcag aaatgggggc aacgtgcaaa acttgccctt
     4621 gctggtgaac aacgtcttac aaagatggtt cctggatgga ttgaccctga gacttttaaa
     4681 ctcaatgaac acgctgagac tgtgagattg atattcaaac tgctgctaga tggtgaaagt
     4741 ctgcataaca ttgcacgtca ccttcaaagc aacggtataa agtcgtttag tcgccgtaaa
     4801 gatgctaatg ggttctctgt tcactctgta cgcaccattc taaggtcaga gacaacaata
     4861 ggcacgttac cagcatcaca acgtaatgac cgccccgcta taccgaacta ctacgaaggt
     4921 gttgtagata taccaacgtt caataaagct caagagattc tcgacaagaa tcgtaaaggc
     4981 cgtacacctg caagtgacaa cccactaacg attaacatct tcaaaggtct gtttaggtgt
     5041 cagtgtgggg ctagtgtcca tcctaccgga acaaagaata agtatgctgg ggtctacagg
     5101 tgcaataacc acttagacgg tcgctgtgat gttccaccgt tgaagcgtaa accgtttgac
     5161 cgatggatga ttgataattt tctggggatg attgacgtgg ggaatgatgg agaatcagag
     5221 agaaagattg cagcgttaca gcatgaggtt gaaattgtca cagccagaat caagaaagct
     5281 accgccctac ttcttgagat ggatgatatt gatgaactaa aaattcagct taaggaactg
     5341 aaccagaagc gcacagaact acagaccacg attgataaca tgaggcg
//
LOCUS       DAJTYZ010000205         5210 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000205 DAJTYZ010000000
VERSION     DAJTYZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.205"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..513)
                     /locus_tag="OO596_003914"
     CDS             complement(<1..513)
                     /locus_tag="OO596_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6719639.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="OO596_003915"
     CDS             complement(759..1211)
                     /locus_tag="OO596_003915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6719640.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OO596_003916"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OO596_003916"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6719641.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OO596_003917"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OO596_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6719642.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="OO596_003918"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="OO596_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..>5210)
                     /gene="rayT"
                     /locus_tag="OO596_003919"
     CDS             complement(4926..>5210)
                     /gene="rayT"
                     /locus_tag="OO596_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6719643.1"
                     /translation="DFSSRWREIKKQFTHACGLKNIWQPRFWEHAIRNTKDYRHHVDY
                     IYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAGERIIL"
BASE COUNT         1227 a         1372 c         1367 g         1244 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc
//
LOCUS       DAJTYZ010000206         5163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000206 DAJTYZ010000000
VERSION     DAJTYZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.206"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            12..428
                     /gene="soxR"
                     /locus_tag="OO596_003920"
     CDS             12..428
                     /gene="soxR"
                     /locus_tag="OO596_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6719644.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="OO596_003921"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="OO596_003921"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6719645.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="OO596_003922"
     CDS             2475..4124
                     /locus_tag="OO596_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6719646.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5163)
                     /locus_tag="OO596_003923"
                     /pseudo
     CDS             complement(4278..5163)
                     /locus_tag="OO596_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1117 a         1202 c         1393 g         1451 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tag
//
LOCUS       DAJTYZ010000207         5114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000207 DAJTYZ010000000
VERSION     DAJTYZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.207"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..379
                     /locus_tag="OO596_003924"
     CDS             <1..379
                     /locus_tag="OO596_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719647.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="OO596_003925"
     CDS             complement(575..2125)
                     /locus_tag="OO596_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6719648.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="OO596_003926"
     CDS             2297..2743
                     /locus_tag="OO596_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCR6719649.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="OO596_003927"
     CDS             complement(2937..3872)
                     /locus_tag="OO596_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6719650.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="OO596_003928"
     CDS             4384..>5114
                     /locus_tag="OO596_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719651.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADEC"
BASE COUNT         1418 a         1244 c         1126 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa gaagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAJTYZ010000208         4888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000208 DAJTYZ010000000
VERSION     DAJTYZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.209"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(67..143)
                     /locus_tag="OO596_003929"
     tRNA            complement(67..143)
                     /locus_tag="OO596_003929"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(107..109),aa:Arg,seq:tct)
     gene            complement(157..233)
                     /locus_tag="OO596_003930"
     tRNA            complement(157..233)
                     /locus_tag="OO596_003930"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Arg,seq:tcg)
     gene            complement(241..316)
                     /locus_tag="OO596_003931"
     tRNA            complement(241..316)
                     /locus_tag="OO596_003931"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(281..283),aa:Ile,seq:cat)
     gene            complement(357..1415)
                     /locus_tag="OO596_003932"
     CDS             complement(357..1415)
                     /locus_tag="OO596_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR6719652.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1523..1763)
                     /locus_tag="OO596_003933"
                     /pseudo
     CDS             complement(1523..1763)
                     /locus_tag="OO596_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1988..2809)
                     /locus_tag="OO596_003934"
     CDS             complement(1988..2809)
                     /locus_tag="OO596_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6719653.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(2806..3186)
                     /locus_tag="OO596_003935"
     CDS             complement(2806..3186)
                     /locus_tag="OO596_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6719654.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(3187..4173)
                     /locus_tag="OO596_003936"
     CDS             complement(3187..4173)
                     /locus_tag="OO596_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6719655.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            complement(4247..4519)
                     /locus_tag="OO596_003937"
     CDS             complement(4247..4519)
                     /locus_tag="OO596_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719656.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            complement(4823..>4888)
                     /gene="hokD"
                     /locus_tag="OO596_003938"
     CDS             complement(4823..>4888)
                     /gene="hokD"
                     /locus_tag="OO596_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6719657.1"
                     /translation="EVRIRTGQTEVAVFTAYEPEE"
BASE COUNT         1143 a         1381 c         1125 g         1239 t
ORIGIN      
        1 tcaaccgtct gaattgtgag acatttaaaa aaaaggcccg caaaagcgag ccagggaaaa
       61 taagtgtggc gcgttgtact ggattcgaac cagtgaccga ttgcttagaa ggcaattgct
      121 ctgtccggct gagctaacaa cgcatgatgc agataatgga ccgccatcgg ggacttgaac
      181 cccgcgcagc cagcttcgaa ggctggcgct ctttcctgat gagctaatgg cggtatgtga
      241 tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct cgctctcacc
      301 actgagctaa agggccggga gccgcataat aacgacgcgt aattaattct tcaatatcat
      361 ccgttctggc tgactaaatc ctgtacttcc cgaaccgtct gctcaaaacg ttcagtctcc
      421 agctcaacgc caattgcacg acgcccgagc gccagtgccg ctttcactgt cgaacccaac
      481 cccatgaaaa aatctgcaat caggtcaccc ggacgactgc tagcgctgat tatctgctgc
      541 agcatttctg ccggtttttc gcacggatgt ttcccgggat agaactgcac cggtttatgc
      601 gtccacacat cggtatacgg cacctgcacc gtcacgccaa aataccgccg cagatgttta
      661 tattcactca gcagctccgc atactgccgg ttcagtgagg tatacgtatc caccagctgg
      721 tggtggagct tttccagttc accacgccga tgcttctctt ctgccacccg ggcaaacagc
      781 gcctgtaatt tcagataatc gctttcattc ggcaactgcc actgaccggc actgaaccag
      841 tgcgacacca tgtttttctt tcctgtggca tctgcaatct gttttgccgt tatccccagg
      901 gcagctcgcg catcacgaaa gtaagaaatc agcggggcca tcacatgctg tttcagtgca
      961 ctgcccttcg ccgcataccc ggcatctttc ggacgatacg gcccctgata atgttccgcg
     1021 aacagaatgc gctctgtggc ggggaaatac gcccgcaggc tttccttgtt gcatccgttc
     1081 cagcgtccgg acggcttcgc ccagataata tggttcagca cactgaagcg ttcacgcatc
     1141 atgatttcga tatcagatgc caggcgatga ccacagaaca ggtaaagact tccggcaggt
     1201 ttcagcaccc gccagaactg cgccagacac tggtccagcc acttcaggta atcatcgtcg
     1261 cccttccact ggttatccca gccctcaggc ttcactttaa agtacggcgg gtccgtgact
     1321 atcaggtcaa cagaattttc gggtaacgac cggataaatt ccaggcagtc ggcgttgatt
     1381 aactcacaac tggatatttt tacagtatta agcatggatc attaagcctg tctctgatag
     1441 gctcattctg cttttgcgca aagcagtggg cctgaggttt gcttgtgaac ccaacgcatg
     1501 agcagatggc tggtgggtgc ccctaacacc caccagccgc ccatttacca caaataaaaa
     1561 agccttcact gcggaaggcg tctgtaacaa ccgaactgat agtctgccag acccgccata
     1621 accagctggg tcagtattaa ctggcagcgt tcgcgtgaaa ggtaagtatt ctgcgctatc
     1681 tccccgactg tcgccggttc ggtaacgctt aattcattaa acaccactct ggcggtttct
     1741 gtcatatcct gctgttttag catgtctttt ccttttccgg ttaacgtgac acaccaataa
     1801 ctcttgtcga aaaagccagc aagctgaaag acaggtattc accgccacca gcgcgtttac
     1861 tgtactgacg cgatttcagt cataaaaaac ccgctcggcg gcgggttgta gaaactcttc
     1921 taacgtcagg cataaaacgc ccatcgttat gacgaattta ccacagattc cggaaaaatc
     1981 aaccttgtta cctagttacc ttttttaact gccgctcagc ccatgcttct tcaatatcaa
     2041 accgggtcac cagcgcatca taaaatttct taactgtttt ttcccatgac gcgcgtgtta
     2101 tctggtttgt cacctcgcat atagcattaa atgcctccgt tgatggtagt ctttcatagc
     2161 cacgaccacc acaacgctgg cagtctctga taacaggcat accacgtttt accgactctt
     2221 cacgatgaat ggcgacacca cgcccacggc aatccttaca ggcggtggaa acctcaccct
     2281 ttccgccaca ctccggacag gcaactttta ccacctccct gacttttttc cattcttccc
     2341 agtaagacgg atacacacct ttcgtacact ttgcccatac cggcggctta ccatccggat
     2401 actggatctt gtttgtaaaa acctcgcttt caataaattt ttttccgtga cagcaggggc
     2461 actgtttttt gctcgccgcg ctacgggcat aatcttcaaa cgcatacgaa gccataatac
     2521 gcatcactgc cggttttatt tctgccggga gttttcttaa cgccgccacg cgatcacacc
     2581 gactgagtgc atattctgtc agcaattctg ttgcccgctc tctgtcattc atactaatgc
     2641 ccattttccc aaggaacgca gaaaccccca tctcagcccg attctgtgtc atgccctgcg
     2701 cggccatcac atcagtgata ctcagcgcat ctttcgacgt tgaggccgat gcatcagtca
     2761 ggccggggga ttttggggag tagtatttcg gtaaatcttc cagtttcatt ttttgacctg
     2821 cccttcaagc attatgggat aaatcttcac ccccagacgt ccaccagata ctggctgagc
     2881 acgaacgata ttgatttcat caaactgctc atcgtccatt agcaaccccg catgcgtcag
     2941 cgcatccagc ggcgctttca gaatattgtc caggtcacgg cggcgcttat ccggtggttc
     3001 tgcaataatt tttattgcca accttccgga caggcttaat ttcagccgct gctggcgaac
     3061 aataagcgcc actgcccggc gataacgctc cccggctttt gatacaaaat atgtgctgcc
     3121 acgacgacgc cagtaagtgt tcaccgtcgg cgggtaaggt aaaacaaatt ctatgcgctc
     3181 ggtcatttat gctttccact tcagaacacc cgaatttctc gcgtgcatta aaaaacgaat
     3241 cagcaacaac agctgactgc cgtgtttctc ttcaaaatct tttactccgg catgtagttc
     3301 gttacggcat ttacggcata acggaataac aaacaaatca tcagcctttg ttcccatccc
     3361 tcccagtcca tgaccaatga tgtgatgcgg atcatctgcc tgattgccac acgtcatgca
     3421 tttctgcgtt tttacccagc gcgtgtatac aggcatctct tcccgttgtg atttctggcg
     3481 ctggagatac tgagccggtg actccggatc aacggcaatg ctgaccaccg tcttttcctg
     3541 tggtgggttc tgttgctggt ggacgtgagg cagtaacgca atattttttg tgcgctgctt
     3601 cagtatgctg gtggctgtct gctctcccgg tatgatgtcg ctttcactgt acacagagcg
     3661 aattttttcc gcacgcaacc ccagcgaacg acgtaatacc gcttccggta gcgcgtccgc
     3721 cacctgattg cggaccgccc accaggataa ttcagccaga gataattcac gctcctgcgt
     3781 accgcttatt gcgtgaccga tgacgtcaat catccatgct gacaggtttt gatgagcaag
     3841 ttgctcgagt gattcggatg tctggtcacg cagctggttg tcgcagtgcc agcacaacac
     3901 cattgcgccg gtaccataac ggtgaatgac ggtttcgctg tgatgataat cgccgtgtgg
     3961 ccactggcag gatttaatat ggcgcaacag ccagtcagac aatgcaccag caccaccagc
     4021 agcacgaatc acccgtgcgt tactgaaaaa cggcagcaat gttttgtctt ccaccagcgg
     4081 ctggcgagcg gcaggaacga ccccggacgg cagattacgc atgctttttg gttccggctc
     4141 caccagtatt ctgccgttat ggaatgctga cattgattca cggcctggct taacgataac
     4201 cagaccgagt tccggtacca gaacaggtcg aagtaatacc cgcacattac ctccagatcc
     4261 gttgctggaa tgtgcgggac ggacgcgttg gccgttcgga gtaagggagc ctgacggaga
     4321 ttatccagtg acggtagtcg aggctaaggg cttttttaac ctcgcatccg cgcctgcggt
     4381 aacactgaat gagccattcg gcctgttctt cagtgcatgg gggatgctgg taccaatcag
     4441 atttaaatgc gtgagagcgc cgcccgtgcc tgctggcaaa gacggctgaa ttatcagaat
     4501 tgtgtagtct ggaaggcagt ggctgacaat agcgccattc tcaacgcgca cagtatagag
     4561 gccatcttca ctaaaaattt cacgtaattc ttcgattttc atcaacagaa tccttccaga
     4621 taaatagcac tcccctgttc ggggtccatc cctcttctcc ctgcgcgcta cttaagtatt
     4681 tttgattcta ttccggcacc atctaaaact tcaaacgcgt tgaaaataaa aacaaaaacc
     4741 cgccgaagcg ggttaagtgt gggtgcattg aggatgcctg acccatcaga ggtggcgagg
     4801 gatttctccc ccgcctggtc tcttactcct caggttcgta agctgtgaag acagcgacct
     4861 ccgtctggcc ggttcgaatt cgtacctc
//
LOCUS       DAJTYZ010000209         4804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000209 DAJTYZ010000000
VERSION     DAJTYZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.210"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(279..1322)
                     /locus_tag="OO596_003939"
     CDS             complement(279..1322)
                     /locus_tag="OO596_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6719658.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1734..2918)
                     /locus_tag="OO596_003940"
     CDS             complement(1734..2918)
                     /locus_tag="OO596_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6719659.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3218..3312)
                     /locus_tag="OO596_003941"
     tRNA            complement(3218..3312)
                     /locus_tag="OO596_003941"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3276..3278),aa:Sec,seq:tca)
     gene            3605..>4804
                     /locus_tag="OO596_003942"
     CDS             3605..>4804
                     /locus_tag="OO596_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR6719660.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1253 a         1056 c         1092 g         1403 t
ORIGIN      
        1 gggataccag ttcaaccgaa aacgccagag gaggggatta cccgctggca gggtaaatct
       61 gtggtttttg atgccagcta taacgtgttt gacgataaag tattacgaaa gaactttaat
      121 gagttcatgg agtaattcgt taaagtaatt tacaacgcta tccttttgca aaggaaactt
      181 tcagaaaata accccagggt aatcacctgg ggaaggaaca cagtgttatt attggataat
      241 aaattattac ttgttttaaa gttacactaa tgcaatatct actgatactg gatgcttgaa
      301 tgtttcatcc ctgctgcgga aaagggcaat atccagggcc agatatattc accgttcata
      361 tttctgcatt tctgtacaat ttcctcagaa agtgtcttgt caattctttt ccacaggtgc
      421 tcctgctgtc ttaaaatact ttcgcatatt tcgaaatcat cttcctgaat cagcatttcg
      481 gcgcatttaa tgatttctga tcgttcatgc agcacggagg aatggttata gagccagttt
      541 acaaacagca atgcttccgg atattgtccc cataatggtg agtatgttgc atagtaatct
      601 tctgccgtat ccagagagcc ggagaagtca tgccacacag caagtggaaa tggatgcaga
      661 tttccgcaca gcatttctgc gatgtaagtg tttgtggcaa agccatggcg aaaagctgtg
      721 gcggccttaa catgttcccc gttcagaaaa tgcaggagac cgagttcata ccagtaaggt
      781 gaaaattcat cagcatgctc tttcagtaca ctgaaggctc gctcatgatc gccggtgcgt
      841 aacagctctg aacccaacaa ccagcgagcc ccctgattat cttcaggatt gtatgccagt
      901 attttatcag tgagcataac cgcatcctga tggcgctgta agtgcacatt tgcgagaatt
      961 gctgcataaa gcgcgcgcag ataaggccgg ttttcgggat gcatccagat tatttcacca
     1021 cagaaacttt ccgggatgat acgattacct gccgccagtc ctttaagaga ggcgttcagt
     1081 gccttacgcg gggcattctg ttccagaaat gcatatgcca gatgagcgtg tatatcaata
     1141 aagtccggct cccgttgagc aaggcgtcgg agtttgttga tgtaactcgt ctcgctgatt
     1201 tcatcagcgt cgtatgcatc aagcacttct tcaaactccc ccagcaatgg gccaaaccag
     1261 gtatccggaa atacgaaata acccttgtta tcgtgcactt caaagcataa tctatcgttc
     1321 atttctgagc ctgtaaaaca tgtggtgata tagtctgaga gcataaaagt tgagattctg
     1381 tgtatcaagt cctgtcttgg caccgaatga atattgctta cgaagcccct tcatgctttc
     1441 cttgagaaca ataattttat ctttatgtga atctcatttt ttataatgat gtcagatgat
     1501 tgaaatgcta aattatatct gaatcaactt ttcctttgct aaaggtatcg aaataactgc
     1561 aaggctgtat tttgtaattg ccaatttaaa aatgtcaatt tgtttttaat tatgtttttg
     1621 taataagttt gaagacgcaa aaaaaataat ataataacaa aaatcctcca gccttgtagg
     1681 ccagaggata aaatacttaa ttgaggaaaa tatcagggaa aagtatttct gacttaactt
     1741 tcccggctgg agagtgaatc aatgtaatca gcgtaccatt gcatcatttc acggcggcca
     1801 tcgagataca gggcatgatt ataggtaccc ctgatggaat ttttatccac atgagctaac
     1861 tgcatttcaa tccaggcgga attaaagcct tgttcatgca gaattgtact catggtgtgt
     1921 ctgaaaccgt gccctgtgag tcttccctta tatccaagta attcaattac ttggttaatg
     1981 ctttccttac taattggttt gcggggatca tttctaccga taaagactag gggatgatgt
     2041 tttgagattg gttcaagctg cttaaatagc ataatgacct gctcagagag cggcacaata
     2101 tgaggtcgct tcatcttcat tacttctgcc gggatctccc acaattttgt ctcaaaatcg
     2161 atatcttccc agcgtgcaaa acgcagttct tgtgttcgta cgccggttaa cataatgatc
     2221 tgagtagctg tcttagtgat tatacttccg gtataacccg ccagatcgtt gaggaagtgg
     2281 ggaagttcat tagcagtaag aaagggaaaa tgtacttttt tcggtgtagc taaagcactg
     2341 gcaagatcgg gggctgggtt atagtctgcc cggccagtaa caattgcata gcggaacacc
     2401 tcaccacagc gctggcggac cttgcgcatt ttctccagag ctccacgttt ttccatttta
     2461 cgcagtgctt ccagtaactc cattggtttg atttcggcaa tagggcgctt gccaatgtag
     2521 ggaaaaatgt ctttttcaaa ggtatcaatg atttcatcac gatagcgtaa tgaccaccga
     2581 tcagcttttg actggtgcca ttcgcgagca atagcctcga acgtattttt cagactcatt
     2641 tgctgcgcga ttttttcttc cttttttgcc tcccctggat cgcctccagc agcgaggagt
     2701 ttcctggctt cactgcgttt ctcgcgagca tcggctagcg ttacatcggg gtatacgcca
     2761 atagaaagca tcttttcttt accagcaaag cggtacttca tgcgccagta tttagaacca
     2821 ttagtgttaa tcagtagata cataccgccg ccatcagcaa gcttataggg tttgtctttg
     2881 ggttttgcgg ttttcacttt gatatcggtt aatgccatga catcctccgg cttttgatga
     2941 attcatgttc ctgaggggga tttctgccag taactaacct gataccccca atacacccct
     3001 tgtataacaa agatattgtt aaaagccgtt gttatgtatc acatttttcc tattatgggg
     3061 gtgcttgtat ttcaatagga aataacaccc cctaaagtac ccccaaaaga aacttgatat
     3121 ggggtgaagt aggttgacct caggatacaa ataaatactg atatacaagg gattagaaga
     3181 gatttattga cattgggaga gttcagaaga ggtaaactgg cggaagatca caggagtcga
     3241 acctgcccgg gaccgctggc ggccccaact ggatttgaag tccagccgcc tcaccggaga
     3301 cgacgatctt ccgcgcctcg attgctacat ggaggcgggg cgcattatag ctacttcctt
     3361 gagtttctac atcccccaga tcagtttttt ccgcctaata cacgcagcgt attatcacga
     3421 aagcattata actcatataa aacccatatt ttacaatgat atatgagcgg aattaacgct
     3481 accacgatca cataaaacaa gaatcataaa ttaataacca gatatcggaa tattcgctct
     3541 cacagggatg gtttacaaaa tgcgttatcg gtctcagcac ccctatagca tcaaggaaaa
     3601 gcagatgaag agtgaagtgt tgtccgttaa agagaaaatt ggttatggca tgggagacgc
     3661 cgccagccac attattttcg ataacgtaat gttatatatg atgttctttt ataccgatat
     3721 ttttggcatt cctgccggat ttgtcggaac catgtttttg gtcgctcgtg cactggatgc
     3781 gatttccgat ccttgcatgg ggttgttggc cgatcgaacg cgctctcgct ggggtaaatt
     3841 tcgtccgtgg gtactgtttg gcgcactgcc attcgggatc gtctgtgtac tggcctatag
     3901 cacgccagat ctcagtatga acggcaaaat gatctatgca gcaattactt acaccctact
     3961 taccttactt tataccgtcg tcaatatccc ttactgcgca ttgggtggtg taatcaccaa
     4021 tgacccgact cagcgtatct cgctgcaatc ctggcgtttt gtgctggcga ccgcgggagg
     4081 catgctttct actgttctga tgatgccact ggttaattta attggcggtg ataataaacc
     4141 actcggtttc cagggcggta tcgcggtcct ttccgtggtg gcattcatga tgctggcatt
     4201 ttgtttcttc accactaaag aacgcgttga agcaccacct acaacaacgt ctatgcggga
     4261 agatttacgt gatatctggc aaaacgacca gtggcggatt gtcggtttac taaccatttt
     4321 caatatcctg gcggtgtgcg tacgcggtgg ggcgatgatg tattacgtca catggatttt
     4381 gggcacgccg gaagtgtttg tcgcttttct caccacttat tgcgtgggta acctgattgg
     4441 ttccgcactg gcaaaacctc tgaccgactg gaaatgtaaa gtcactatct tctggtggac
     4501 gaacgccctg ctggcagtga ttagcctcgc gatgttcttt gttcccatgc aggccagcat
     4561 cactatgttt gtcttcatct tcgtgattgg tgtgttgcat caactggtga cacctatcca
     4621 gtgggtaatg atgtccgata ccgtcgacta cggcgagtgg tgcaatggta aacgcctgac
     4681 cgggatcagt tttgctggca cgctgtttgt gctcaaactg gggttggcct tcggcggcgc
     4741 tcttatcggc tggatgctgg cttatggcgg atatgatgcg gcagaaaaag cgcagaacag
     4801 cgcc
//
LOCUS       DAJTYZ010000210         4799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000210 DAJTYZ010000000
VERSION     DAJTYZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.211"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="OO596_003943"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="OO596_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6719661.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="OO596_003944"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="OO596_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR6719662.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="OO596_003945"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="OO596_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6719663.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="OO596_003946"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="OO596_003946"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6719664.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="OO596_003947"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="OO596_003947"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6719665.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..>4799
                     /locus_tag="OO596_003948"
     CDS             4447..>4799
                     /locus_tag="OO596_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6719666.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENAST"
BASE COUNT         1311 a         1229 c         1096 g         1163 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaat
//
LOCUS       DAJTYZ010000211         4778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000211 DAJTYZ010000000
VERSION     DAJTYZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.212"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(51..494)
                     /locus_tag="OO596_003949"
     CDS             complement(51..494)
                     /locus_tag="OO596_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6719667.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            651..1580
                     /gene="metA"
                     /locus_tag="OO596_003950"
     CDS             651..1580
                     /gene="metA"
                     /locus_tag="OO596_003950"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6719668.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1849..3450
                     /gene="aceB"
                     /locus_tag="OO596_003951"
                     /pseudo
     CDS             1849..3450
                     /gene="aceB"
                     /locus_tag="OO596_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3480..4771
                     /gene="aceA"
                     /locus_tag="OO596_003952"
                     /pseudo
     CDS             3480..4771
                     /gene="aceA"
                     /locus_tag="OO596_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1189 a         1259 c         1209 g         1121 t
ORIGIN      
        1 tgcgacgctg gcgcgtcttc tccagcctgg gcaggcacgg caggggcagc ttattcctcc
       61 acatacgcca gattcagcaa cggatacggt ttccccaaat cgtccacctc agagcgtccc
      121 gtaaccttaa aacccacctt cttatagaac ccaaccgcct gctcattttg ctcattaacg
      181 ttggttgtca gttccggtgc catcgagagc gcatgctcca ccagcatccg acccacgccg
      241 cagccgcaca catcaggatc gataaacagc gcatccatat gctgcccact taacaacata
      301 aacccaaccg gctgatcccg ttcattaacc gcgacccaca acggcgcttc cggcaggaag
      361 gaacgaacta gcacctccag ctcggcccga tactctgctg ataaaaaatc gtgtgtggca
      421 tcgacagaac gacaccaaat cgcaacgagt tcctcccctt cctcatgccg tgagcggcga
      481 atactaataa ccattttctc tccttttcgt cattcttata ttctaacgta gccttttcct
      541 tgaaactttc tcaccttcaa catgcaggct cgacattggc aaattttctg gttatcttca
      601 gctatctgga tgtctaaacg tataagcgta tgtagtgagg taatcaggtt atgccgattc
      661 gtgtgccgga cgagctaccc gccgtcaatt tcttgcgtga agaaaacgtc tttgtgatga
      721 caacttctcg tgcgtctggt caggaaattc gtccacttaa ggttctgatc cttaacctga
      781 tgccgaagaa gattgaaact gaaaatcagt ttctgcgcct gctttcaaac tcacctttgc
      841 aggtcgatat tcagctgttg cgcatcgatt cccgtgaatc gcgcaacacg cccgcagagc
      901 atctgaacaa cttctactgt aactttgaag atattcagga acaaaacttt gacggtctga
      961 tagttaccgg cgcgccgctg ggcctggtgg agtttaatga tgtcgcttac tggccgcaga
     1021 tcaaacaggt gctggagtgg tcgaaagatc acgtcacctc gacgctgttt gtctgctggg
     1081 cggtacaggc cgcgctcaat atcctctacg gcattcctaa gcaaactcgc accgacaaac
     1141 tctctggcgt ttacgagcat catattctcc atcctcatgc gcttctgacg cgtggctttg
     1201 atgattcatt cctggcaccg cattcgcgct atgctgactt tccggcagcg ttgattcgtg
     1261 attacaccga tctggaaatt ctggcagaga cggaagaagg ggatgcatat ctgtttgcca
     1321 gtaaagataa gcgcattgcc tttgtgacgg gccatcccga atatgatgcg caaacgctgg
     1381 cgcaggaata tttccgcgat gtggaagccg gactagaccc ggatgtaccg tataactatt
     1441 tcccgcacaa tgatccgcaa aacaaaccgc gcgcgagctg gcgtagtcac ggtaatttgc
     1501 tgtttaccaa ctggctcaac tattacgtct accagatcac gccatacgat ctacggcaca
     1561 tgaatccaac gctggattaa tcttctgtga tagtcgatcg ttaagcgatt cagcacctta
     1621 cctcaggcac cttcgggtgc cttttttatt tccgaaacgc acctcagcaa gtgaataaat
     1681 tttattcata ttgttatcaa taagttatca agtattttta attaaaatgg aaattgtttt
     1741 tgattttgca ttttaaatga gtagtcttag ttgtgctgaa cgaaaagcgc acaacgatcc
     1801 ttcgttcaca gtggggaagt tttcggatcc atgacgagga gctgcacgat gactgaacag
     1861 gcaacaacaa ccgatgaact ggctttcaca aggccgtatg gcgagcagga gaagcaaatt
     1921 cttactgccg aagcggtaga atttctgact gagctggtga cgcattttac gccacaacgc
     1981 aataaacttc tggcagcgcg cattcagcag cagcaagata ttgataacgg aacgttgcct
     2041 gattttattt cggaaacagc ttccattcgc gatgctgatt ggaaaattcg cgggattcct
     2101 gcggacttag aagaccgccg cgtagagata actggcccgg tagagcgcaa gatggtgatc
     2161 aacgcgctca acgccaatgt gaaagtcttt atggccgatt tcgaagattc actggccccg
     2221 gactggaaca aagtgatcga cgggcaaatt aacctgcgtg atgcagttaa cggcaccatc
     2281 agctatacca atgaagcagg caaaatttat cagctcaagc ccaatccagc ggttttgatt
     2341 tgtcgggtac gcggtctaca cttgccggaa aaacatgtca cctggcgtgg tgaggcaatc
     2401 cccggtagcc tgtttgattt tgcgctctat ttcttccaca actatcaggc actgttggca
     2461 aagggcagcg gtccctattt ctatctgccg aaaacccagt cctggcagga agcggcctgg
     2521 tggagcgaag tcttcagcta tgcagaagat cgctttaatc tgccgcgcgg cgccatcaag
     2581 gcgacgttgc tgattgaaac gctgcccgcc gtgttccaga tggacgaaat ccttcacgcg
     2641 ctgcgcgacc atattgttgg tctgaactgc ggtcgctggg attacatctt cagctatatc
     2701 aaaacgttga aaaactatcc cgatcgtgtc ctgccagaca gacaggccgt gacgatggat
     2761 aaaccattcc tgaatgctta ctcacgcctg ctgattaaaa cctgccataa acgcggtgct
     2821 tttgcgatgg gcggcatggc agcgtttatt ccgagcaaag atgaagagcg taataaccag
     2881 gtgctcaaca aagtaaaagc ggataaagcg ctggaagtca ataacggtca cgatggcaca
     2941 tggatcgctc acccaggcct tgcggacacg gcaatggcgg tattcaacga cattctcggc
     3001 tcccgtaaaa atcagcttga agtattgcgc gaacaagacg cgccgattac tgccgatcag
     3061 ctgctggcac cttgtgacgg tgaacgcacc gaagaaggta tgcgcgccaa cattcgcgtg
     3121 gctgtgcagt acatcgaagc gtggatctcc ggcaacggct gcgtgccgat ttatggcctg
     3181 atggaagatg cggcgacggc tgaaatttcc cgtacctcga tctggcagtg gatccatcat
     3241 caaaaaacgt tgagcaatgg caaaccggtg accaaagcct tgttccgcta gatgctgggc
     3301 gaagagatga aagtcattgc cagcgaactg ggcgaagaac gtttctccca gggccgtttt
     3361 gacgatgccg cacgcttgat ggaacagatc accacttccg atgagttaat tgatttcctg
     3421 accctgccag gctaccgcct gttagcgtaa accaccacat aactatggag catctgcaca
     3481 tgaaaacccg tacacaacaa attgaagaat tacagaaaga gtggactcaa ccgcgttggg
     3541 aaggcattac tcgcccatac agtgcggaag atgtggtgaa attacgcggt tcagtcaatc
     3601 ctgaatgcac gctggcgcaa ctgggcgcag cgaaaatgtg gcgtctgctg cacggtgagt
     3661 cgaaaaaagg ctacatcaac agcctcggtg cactgactgg cggtcaggcg ctgcaacagg
     3721 cgaaagcggg tattgaagca gtctatctgt cgggatggca ggtagcggcg gacgctaacc
     3781 tggcggccag catgtatccg gatcagtcgc tctatccggc aaactcggtg ccagctgtgg
     3841 tggagcggat caacaacacc ttccgtcgtg ccgatcagat ccaatggtcc gcgggcattg
     3901 agccgggcga tccgcgctat gtcgattact tcctgccgat cgttgccgat gcggaagccg
     3961 gttttggcgg tgtcctgaat gcctttgaac tgatgaaagc gatgattgaa gccggtgcag
     4021 cggcagttca cttcgaagat cagctggcgt cagtgaagaa atgcggtcac atgggcggca
     4081 aagttttggt gccaactcaa gaagctattc agaaactggt cgcggcgcgt ctggcagctg
     4141 acgtgacggg cgttccaacc ctactggttg ctcgtaccga tgccgatgca gcggatctga
     4201 tcacctccga ttgcgacccg tatgacagcg aatttattac cggcgagcgt accagtgaag
     4261 gcttcttccg tactcatgcg ggcattgagc aagcgatcag ccgtggcctg gcgtatgcgc
     4321 catatgctga cctggtctgg tgtgaaacct ccacgccgga tctggaactg gcgcgtcgct
     4381 ttgcacaagc tatccacgcg aaatatccgg gcaaactgct ggcttataac tgctcgccgt
     4441 cgttcaactg gcagaaaaac ctcgacgaca aaactattgc cagcttccag cagcagctgt
     4501 cggatatggg ctacaagttc cagttcatca ccctggcagg tatccacagc atgtggttca
     4561 acatgtttga cctggcaaac gcctatgcct agggcgaggg tatgaagcac tacgttgaga
     4621 aagtgcagca gccggaattt gccgctatac cttcgtatct caccagcagg aagtgggtac
     4681 aggttacttc gataaagtga cgactattat tcagggcggc acgtcttcag tcaccgcgct
     4741 gaccggctcc actgaagaat cgcagttcta agcaaccg
//
LOCUS       DAJTYZ010000212         4760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000212 DAJTYZ010000000
VERSION     DAJTYZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.213"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..279
                     /locus_tag="OO596_003953"
     CDS             <1..279
                     /locus_tag="OO596_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6719669.1"
                     /translation="YTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWK
                     NRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA"
     gene            620..2272
                     /locus_tag="OO596_003954"
     CDS             620..2272
                     /locus_tag="OO596_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6719670.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2283..2453)
                     /locus_tag="OO596_003955"
                     /pseudo
     CDS             complement(2283..2453)
                     /locus_tag="OO596_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2586..2705)
                     /locus_tag="OO596_003956"
                     /pseudo
     CDS             complement(2586..2705)
                     /locus_tag="OO596_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2779..3213)
                     /locus_tag="OO596_003957"
     CDS             complement(2779..3213)
                     /locus_tag="OO596_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HCR6719671.1"
                     /translation="MDTFKRRNVATSFSFLGYDFKVRTLKNFKGELYRKCMPGASNAA
                     MCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSR
                     LLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            complement(3390..4699)
                     /gene="ltrA"
                     /locus_tag="OO596_003958"
                     /pseudo
     CDS             complement(3390..4699)
                     /gene="ltrA"
                     /locus_tag="OO596_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1273 a         1151 c         1111 g         1225 t
ORIGIN      
        1 tacacacagc ggcttaagga agccggatta ctggcatcaa caggaagtac aggcgactcg
       61 tatgacaacg cgatggcgga gagcatcaat ggtctttaca aagcggaggt aatacaccgt
      121 aagagctgga aaaaccgtgc agaagtggaa ctggccacac tcacgtgggt ggactggtat
      181 aataatcgac gattgctgga aaggctgggc cacatccctc cggcagaagc agaaaaagct
      241 tattatgctt ctatcggaaa cgatgatctg gcagcctgag ttcacagata aaacattctc
      301 caggaaactt ggggcggttc acgtcagtat cagaccatat taattgctga aactgataaa
      361 catcaaacct tatagaaggg tgaatgctga aagggggcta tggtgatcga cgtgatgaca
      421 acgacaggta agaccgtgac ctgataaacc tgaattgtgt cctagacata aagtccgtga
      481 agggaataag gctcaggtca gcgaatctta tagtggcccg cagctctgac tgcaataagg
      541 ccggatacag agcagcaact actcgtcgat gtcagaacaa cggaagcctt gcaaacgggg
      601 atgcgttcat acaggtacaa tgataccgtt aaaaaaaaac attacgctaa taatgttcac
      661 cctgtcatta ctgacaggaa atcctgctat agcgtatgaa acagataaag tatataaaat
      721 aaccgttctg catacaaatg accaccatgg acacttttgg cgtaacaatc atggtgagta
      781 cgggttatcc tcccaaaaaa ctctggtgga caatatccgg cagaaagtaa taaataatgg
      841 cggaagtgtt ctattattat ctggtggtga tattaacacc ggtgtacctg aatcagacct
      901 tcagaaggct gaacccgata ttcgtggaat gaatctaatt ggttatgatg caatggccgt
      961 cggtaatcat gaatttgata atcctctaaa tatattacgt cagcaggaaa aatgggccac
     1021 atttcctttc ctttctgcta acatatatca aaaaagtaca gggaggcgtt tattcagtcc
     1081 atggaaaatt ttcatccgtc agaatttgaa aattgcagtc attggcttga cgacagatga
     1141 tactgcgaaa acaggaaatt ctgaatattt tactgatatt gagttccgtc aacctgctgc
     1201 tgaggcgagg agtgtcattg atgaacttaa tcagcaggaa aagccagata ttattattgc
     1261 ggcaacacat atgggtcact atgacaatgg cgagtcaggt tcgaatgctc ctggtgatgt
     1321 tgaaatggct cgcagtttgc cgactggctc attagcaatg attgtgggag gacattcaca
     1381 agctcctgtc tgcatggcca gcgataataa aaaacagtgg aattatattc cggggacaac
     1441 ctgtgtacca gataaacaaa atggcatctg gattgtacaa gcgcatgagt ggggaaaata
     1501 tgtcggtcag gctgattttg aattctgtaa cggtacaatg aaactggtta attatcagtt
     1561 acacccagtt aatctcaaaa tgagaattac cagagaagat ggaaaaacgg aattttcttt
     1621 ttacaccccc gaaattactg aagatccaca gatgttatcc ttactgacac cattccagaa
     1681 taaaggcaaa gcacaactgg atgtgaaggt tggggttgtg aacggacgtc tggaagggga
     1741 cagaagtaaa gttcgttttg ttcagaccag tatgggccat ctgattctga gcgctctgac
     1801 agaacgtatt gacgcagatt ttgctgttgt cagtggtggg gaaattcgag attccataga
     1861 gagtggtaac atcacataca aagatatact gaaagttcag ccatttggta atacggtagt
     1921 aagtattgat ttgacgggga aagaagtagc tgactaccta gctacagtgg cacagatgaa
     1981 accggattcc ggagcctacc cacaattttt gaataccagc tttgtagtca aaaaaggaaa
     2041 aatcgaaatg ctaaaaataa aaggtaaatc ggttgacctt aacaaaaaat atcgtatgac
     2101 aacatttagc tttaatgcta ctggtgggga tggctatcca cgtattgata atagacccgg
     2161 atacataaat accggattta ttgatgctga agtacttatc gaatatatcc gggaacattc
     2221 accgctggat gctgcttcat atgaaccaaa aggtgaagtc agttggcagt gataacgtaa
     2281 ttgacgatca tcacgtgttt tttgctgaca gtcgctgtga ctttgtgaaa gtagtctctg
     2341 cggatgtttg cgatatggga atgcagttgc tgtattttgt gtttctgctt ctgccagttg
     2401 ttgctgaatt taaccttgcg gcttaactga tgttggagcg ctgccagctt cttcgtcaat
     2461 ttcgctgatt aaagtttatt cattatggca tgatctgcgt aagcggctcg tcagaaccgt
     2521 attgatattt actaagagct cagatcaact ttccagggca acagatcgcg tacccggttt
     2581 gccggtcagt cctggatatg ctcaatgacg taacgcagcc atttttctgg ctccacattg
     2641 ttcagacggc atgtgccgat cagcgagtac aacaccgccg catgttcgcc accgctatcg
     2701 gaaccccgcc ggagagagtt tcactctccg gctgctcacg gaaccgtacg tgacactctc
     2761 gcgtcatacg gctcttgatc actcattcga tgcacgtagt aaataccagt gggcaaatag
     2821 cttcggatac tgtttcctta ccagcgccag ctttcgctga gccttccggt tcgaaagtct
     2881 gtatttagac tgcatccact taagcaaacg tgactgcatt gcactccaca gtcgatagct
     2941 gaagtttctg gaccagaact ttccatagta ctcaatccag cctctcacta tcgcattgta
     3001 gcgcctcgca aaatccagca aactctcagc tgttgagcga tgtatacgcc acttcttgat
     3061 tgtttctgtt attttgcaca ttgctgcatt tgatgcaccc ggcatacatt ttctgtacaa
     3121 ttcgcctttg aaattcttca gagtacgcac tttgaagtca tatccgagaa aggagaaact
     3181 cgtagcgacg tttctccttt taaacgtgtc aatgtaggcg atattcgtct tccctgcgtt
     3241 caggaccaac ccgacttccg agaagcgcac cgataacctg ttcttcagcc ttgttgcatc
     3301 actcattcgt gaacaatgta ctacaagtcg agtcacccga gggaacctcc ccctctggtg
     3361 ctcacggaac cggacgtgaa cctctcagct catccggctc ttctcattga ggcctgtccc
     3421 catagtaacg gccagtgtgc aaacaacctc gtttcactcc gcgcaacacg ggccagccag
     3481 tggctggccc gtcgtcggtg gcccctaagt cgcttgtgtt tacggggcag ccataacacg
     3541 agtttgcgat cgatttgcct cagcgtcaga tacagtgctg atttataaaa agcaccgtag
     3601 tactttatcc agcctctgat tttcgcattg aacatatgcg ccagctcgta caaggacttg
     3661 tcactgcgat tctcctcacc tcctgacgga ttgcttttcc tgctctggca ctcatcgccg
     3721 gactgaagtt aacgaaggtc ttaccccagc gattcatcga cagacgcggc cggaatgtat
     3781 agcccagaaa gtcgaagctc gtttccgggt agtccccccg tcggtcagca tccttacagt
     3841 aaactattcg tgttttttgc ggatgcagct gcagcccgca ttgcgcaagc cgctgcctca
     3901 gtccacttat cagagcgtca gcctgccact ggctgtgaca gtggcataca acatcatcgg
     3961 cgtacctttc gaagggaacg ccgggaaact gacgttgcat ccacatatca aaggcatagt
     4021 gcaggaacag atttgagagc aggggggaga ttacgccgcc ctgcggcgtt cctcgacccc
     4081 gtgccagcat tgctccatcc ggcatttgca ccggtgcctt cagcctgcgc tcaatgtaca
     4141 tcaccaccca gcgtgcctga gtatgatgct gcactgtctt cagtagcagg tcatgatcga
     4201 tatcatcaaa gaagccttta atatcgactt ccaccaccca gtcaaacttc cagcagcgtt
     4261 tcctcgcctg cgcaatcgcc tggtgtgcgg atttacccgg tcgataacca tacgagtctg
     4321 catggaacag gggttccagt accggctcga gtatctgctt aacgaccatc tgggcgatcc
     4381 ggtccgacac ggccggaata ccgagcggac gtacaccacc gtcagctttg ggaatgtcca
     4441 cacgccttac cgcctgcggc atataactgc cggaagccat tctgttccac agtttgtaga
     4501 gattgtctgt aacattctcg tcaaacccgg ccagtgtctg cccgtcaatg cctgccgccc
     4561 cacggttggc ttttactcgt ttataagctt cccatacctg tcgtttactt acaggaaatg
     4621 gctttgccga ttttacagca gtcctcccat cggcattgtc gcccgtggtt gccgccgcac
     4681 catcagcaca atggggcaag ccctttgctc cggacccatt acggtccttc accgctacta
     4741 cggtttactc cgtccctgta
//
LOCUS       DAJTYZ010000213         4757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000213 DAJTYZ010000000
VERSION     DAJTYZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.214"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(67..1011)
                     /gene="mhpB"
                     /locus_tag="OO596_003959"
     CDS             complement(67..1011)
                     /gene="mhpB"
                     /locus_tag="OO596_003959"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCR6719672.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1013..2677)
                     /locus_tag="OO596_003960"
     CDS             complement(1013..2677)
                     /locus_tag="OO596_003960"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HCR6719673.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            2719..3552
                     /gene="mhpR"
                     /locus_tag="OO596_003961"
                     /pseudo
     CDS             2719..3552
                     /gene="mhpR"
                     /locus_tag="OO596_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
     gene            3629..4711
                     /gene="lacI"
                     /locus_tag="OO596_003962"
     CDS             3629..4711
                     /gene="lacI"
                     /locus_tag="OO596_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HCR6719674.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
BASE COUNT         1096 a         1364 c         1305 g          992 t
ORIGIN      
        1 cattcagaaa acggctggtg gcggcttcgg tttgtggctg ataactcatc atcttctcct
       61 gcatattcag ttctctgttc tggcgcttaa cgagccaaat ccggcaatcc actccgggat
      121 tgggcggtaa taacgccctt cgctacgcca gttgccaaat gcagaaatag cggcaaaagc
      181 ggcgacccag gttttgattt catgtgtcga ctttccggca atggcggaaa gctcttcgtt
      241 actgacggca tccagttcct gtatgcgtcc ctgctccagc aaagtcatga actggttatc
      301 ccaaatcggg ttgagcggat gcagagttct ctgatcctca acaaacttct cagcggcgct
      361 aatcaccctt tgctgacgca attcgcgctc actggcgggt aaatctttcc cgctccccaa
      421 cagacggtcg cgcatatggg catcggcttt cgccagttcg ggcaccggcg gctgatggga
      481 aagcccaccg gaacccagga acagcacgcg tttattgaga gtgctggtga aacgtccaat
      541 ggcttcaccc aacatgcggg tacgctggaa accgggcagc ggcgtggcga caccgttgat
      601 gaacacaggc agaactggca ccttatccag cccaccgagc aggaactcca gcggctgggc
      661 gaacccgtgg tccacctgca tacagtaaga aacggcaaga tcgatcccgc tcttcatgac
      721 agcatgcgca caggcctccg ccagctccac aggcacgggc agctctcctg ccgcactgcc
      781 gaaatcacca attgccgtcg ctccaacgcc taaacagaac ggtggcatca cgtcatagaa
      841 aaagccgttg tagtgatctg gcgcaaacag caccaccagt tcaggggaga aagccgcaat
      901 acgctcgcgg gcgctggcaa tcacgccatt gacctcatcg agtacctctt gcgccgggtc
      961 gacatacccc accaacggcg agtgggaaag acagtgaaga taagcgtgca tatcaggcta
     1021 ccttttcgac agaaacgtcg gcatcagggc gggtcagcgt catcaccgac gccagtttat
     1081 tcagcgtatt gcccagggtt tgcggaatgg cggtggcggc aacaaagcga tccgggcgca
     1141 tcaccaccag cgaagcattg tgttgcgcga accagctacg cagacgacct tgcgtatcgc
     1201 ccacgcgtag cacgccgtcg tggttatcct gtgcggtatg aatttgcact tccggcacca
     1261 cctgaatgaa gcgtgtgccc aacgcgcgcc actgctggat ttgctcgtcg ctcatccccc
     1321 acagtggatt gcatccccag ccaattaccg cgaagttcgc gccgatcgcg ttatcgagca
     1381 gcgtcacgtc gccgttttcc agcgtgactt tcggctgaat aaacatcttg ccgaccggag
     1441 agtgcttcgc ttcgccctca cgcaccagcg caccgccgta atattgcggc atcggcttga
     1501 agcgcatttc gaggaagtag cgttttactg gcggcagata attcaacagc caggaaacgc
     1561 cgtcacgtaa cgtaccctgc cagcgtttcg gcggagccag cacgttgccc gccgtcacgg
     1621 acaggtcaat catcgctttg gcgtgatcgc gacgttcttg ttgataggta tcgagcagcg
     1681 catcgcgggc tttcccctgg ataaccaacg ccagtttcca ggcgaggtta aaggcgtcgc
     1741 gcataccact gttatagccc tgcccctgcc acaccggcat gatgtgcgcg gcatcgcccg
     1801 ccagcagtac gcgatcaata cggaaacgtt gcgccagtcg cgcgttatgg gtgtagacac
     1861 gctggcgaat caattcaaca ttgtccggat taggcagcac tttgcttaac agcttgcgca
     1921 tattttgcgg ctcacgcagc tgctcttcgg tttctcccgg catcaccata aattcaaagc
     1981 gacgtaccgc atgaggcagc gcggcagaaa catacgggcg caccggatcg caacacaaat
     2041 agatatgcgg cgtacttaac ggatcgttgg cgatatctac cacaatccac tgatttggcg
     2101 cagttttacc ttcaaacggc acattcaggg tgcgacgaac aaaacttgcc ccaccatcac
     2161 aggccaccag ccactgggct ttgatcgttt cccgctgccc ttctgccgtt ttcaggtgca
     2221 aggtcacttc gtcatcttgc tggctgaagg cctccagctc gcgggaaaac aagcagcgca
     2281 cattcggaaa acgcgacaac ccttccagca tcaccgcatc gacctgtggc tgaataaagg
     2341 cgttacggcg cggccagcca aattcatcgg tcattggctg aatatcagca aaacagcggc
     2401 cttttggggt gagaaaacgc atcgcgtgcc acggtgtagt gtgcggcaga acattctcga
     2461 ccaggccgac cgactgcatg gtgcgcagcg cctcgtcatc aataccaatc gcacgcgggt
     2521 agtcgatcaa cttatcgagt ttctccacca ccagcacgtc aatgcccatc tgaccgagat
     2581 agttcgccat catcagccca accggaccgg caccagcgat cgccacctga acgctatggt
     2641 taacagcagg ctggatgtca gggtgttgta ttgccatttc tgcacaacgt tgttttaact
     2701 atagaaatgt caattaatat gcagaacaat gagcagacgg aatacaaaac cgtgcgcggc
     2761 ttaacccgcg gtctaatgtt attaaatatg ttaaataaac ttgatggcgg tgccagcgtc
     2821 gggctgctgg cggaactcag cggcctgcat cgcaccactg tgcggcgact gctggagacg
     2881 ctgcaggaag agggatatgt ccgccgtagc ccctccgatg acagttttcg tctgaccatc
     2941 aaagtgcggc aattaagcga aggatttcgt gacgaacagt ggatttctgc actggcggcc
     3001 ccgctgctgg gcgatctgtt gcgcgaagtg gtatgaccaa cagatgtgtc caccctggat
     3061 gttgatgcaa tggtggtacg cgaaaccacc caccgtttca gccgcttatc ctttcaccgg
     3121 gcaatggtcg ggcgacgttt accgctcctg aaaaccgcct cgggcctgac ctggctggcc
     3181 ttttgcccgg aacaagaacg caaggaatta atcgaaatgt tagccgcccg ccccggtgat
     3241 gactatcaac tggcacgaga accgttaaag ctgcaagcca ttctggcgcg cgcgcgcaaa
     3301 gagggttacg gacagaacta ccgcggctgg gatcaggagg agaagatcgc ctctatcgcc
     3361 gtaccgctgc gcagtgaaca acgggtgatt ggctgtctga atctggtgta tatggcgagc
     3421 gcaatgacca ttgaacaggc agcggaaaag catcttccgg cgctacaacg ggtagcaaaa
     3481 cagatcgaag aaggggttga atcgcaggct attctggtgg ccggacggcg aagcggcgtg
     3541 catttacgtt gacaccatcg aatggcgcaa aacctttcgt ggtatggcat gatagcgccc
     3601 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgctatacg atgtcgcaga
     3661 gtatgccggt gtctcttatc agaccgtttc tcgcgtggtg aaccaggcca gccacgtttc
     3721 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
     3781 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
     3841 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
     3901 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
     3961 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
     4021 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
     4081 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
     4141 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
     4201 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
     4261 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
     4321 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
     4381 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
     4441 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
     4501 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     4561 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     4621 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4681 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     4741 ctcattaggc accccag
//
LOCUS       DAJTYZ010000214         4712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000214 DAJTYZ010000000
VERSION     DAJTYZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.215"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            134..511
                     /locus_tag="OO596_003963"
     CDS             134..511
                     /locus_tag="OO596_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719675.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            534..686
                     /locus_tag="OO596_003964"
                     /pseudo
     CDS             534..686
                     /locus_tag="OO596_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(742..2358)
                     /locus_tag="OO596_003965"
     CDS             complement(742..2358)
                     /locus_tag="OO596_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6719676.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(2359..3690)
                     /locus_tag="OO596_003966"
                     /pseudo
     CDS             complement(2359..3690)
                     /locus_tag="OO596_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
     gene            3760..4712
                     /locus_tag="OO596_003967"
                     /pseudo
     CDS             3760..4712
                     /locus_tag="OO596_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1278 a         1171 c          998 g         1265 t
ORIGIN      
        1 gaagtttcaa atatcaatgc ccaagatgcc atgccttcag tatggagcct ccggatctac
       61 cggagatgct ttgggactaa tgctccgctt tgcctcgggt atccgttcga cctatgttta
      121 agaggaaacg cacgtgtctg ccgccaaagc tcttgcctat gcactttgtg acaagttgga
      181 aagcaaaaaa gttgatgaag tcgtacgaaa tcttgtttct agcggctgga atattaggga
      241 agtggcctgg gaagcttcgt cctttgatca tgaaatgccc tactttgaca cggggcggga
      301 cttcgatatt ggatttcctg acacgctgtc atccaagatt gagtttttga ccgcgaatgg
      361 atcaatcgtc caagcacgtg ctcgcctgca cttcaagcag gctctaattt ttgccaaggc
      421 gtccaaacat gtcgccgcgc tcaagaactc attggattac tactatggat cgtctgtaat
      481 cccccctcaa aaccacggag gctatttgta gaaaattcca ttaatatgaa ctcaaggtgt
      541 tccctacgga cgaatcagcg atgaaggtgg tgtacctggc tatccaggcg gcgaccaaga
      601 aatggacgat gccgatccgc aactggaagc cggccatgaa ccgttttatg atcgaatttg
      661 gtgaccgttt aaatggccac ctttaactga ccggcaaaag cacttacaca aaagggttta
      721 caggctcttt cagtcattcc tttaatgcgt aaattgctct ctaaccactc tctcagccca
      781 cccttccata tcctctctcg atacttgcag ctcccccaac ccctttggat gattcacccc
      841 tctatgcccg tcttgtccat ataattgatc aaacaaacta tttggccaat tcaatgagct
      901 cttcaccact ccacgccgta tcttttcaaa atcattccgc catgtttcgc tatcaacgcc
      961 actcaacact ttggtagaaa gcattttcac cccatccgat gcatccacct ctagcaatac
     1021 aaattcactg ccattacgtt gcaccccaac ggctttaatc actcttggtg agccatcttt
     1081 acacagatgt aatcggctac gcccaacttt tggcaaaacc aaggtttctt caaaaagcac
     1141 ccgacatgca aatttagttt ttagaattga aagtagctca tcaaaagcag caaatcgatt
     1201 agcaaaaata gagttgtaat tggttgcatc ctgcttccca cccacatctg ccgctgctag
     1261 gacaccacct aagtgtggct catcagtact aaccaaatta ctattttcac ttgttgttgc
     1321 ttcttctttt tctaaaatag tttggctact ttttcttgcc ctgcgagatt tttcaattcg
     1381 actaggtcta ctaaaactta tccatgtcgg ctcagcctct atgactcgtg tgtctgtgtc
     1441 tatattggct gtttcctcat cgtccaactg tagatgctca tcatcaacaa ctgactctgt
     1501 tgaaaccgct atgtgctgcg tactaccatc acctgcctcc ttttcctgaa aagaggcatg
     1561 gctaatagct gttgtgctag gaagcatagc gttgatttct aaacctacta tttcctcaac
     1621 taaataatcc ttatcctcgt tagaagaacg tccttttaca tgtaatttcc aaccctgcat
     1681 gggcggaggg tcaaagctaa agcaccagct ttcaacgccg ttcttaattt ctctattttg
     1741 ttgatagtgc ctaaaaatac tttcatacga atccataaca tcttgatccg aaaacaacca
     1801 aaccaaaagc tgcactacgg ccgactgctc taacgcccct ttaggaaacg atgaactggg
     1861 taagatcctt atctctaaat gatctcgctc aacctcatac tgaacgtcga actcttgctg
     1921 taaagcggta ctgctcaaac agcttcgaca gaaataggag ttaattaaaa ataatgaccg
     1981 agctagctct aactgtggga tgtgataacg agtcttgcta tatggtgttt cagcttcgta
     2041 aataaatgaa ctctgcgctc catccttgtt gcgtacggaa gcaaaggacg agagattagg
     2101 aaaatcgatt attttggctc gttgaagatc cgatgcttca aactcaatca cacgatccgc
     2161 cttatttatt tgctttgttg tactgttaac caccttagct cgacttaata aaggcaaatg
     2221 agtaaatcga gtccatttcc gttcttgcat tggattaaac caaacaaaaa tacgccactc
     2281 cttatgaccc gagttacgga ataaatgacc aatatgtaca acctgcacat tgtcattaaa
     2341 tgtagctagc ctaaccactc actcttcccc ataaaccatt tccaagaatc tttgtgcttc
     2401 ctcagttatc cgctctttag ataacgttgc acttcttaat aatcgccagc gcctaagctc
     2461 aacatcttcc tgtttaagct taataaaagc ttggctaatc cgtctaattt gataatcttc
     2521 cacactctct gagtaacgct ttaagcaaag cgctaccaaa ggcagtttct gtagattttt
     2581 tgcaagagag gttccgttag gagtttgctt taacagccag ctcgatgtcg ctcttgggtg
     2641 atcaaggcta ctatctagac gctttatgat tcttaatagt tgtcgtacag caattcgatc
     2701 tctttggttc caatcaactc taggggcggg tgccagacgc tcttgttgat gctgttgatt
     2761 ccagtgaact agccaatccc tgtcatgtcg gtaaagccaa gcgtatagca ccccaccctc
     2821 taaagactgt cttgccgcct taattccttg gtatttatgc actagttgct gccagtcttt
     2881 atgcttaaca gataaatctt cagagttagg ttgcacagac tggctaacag gtcttgtcgt
     2941 tatagagtgc tcagtaagag cacttgcctg ctgtagcgct tctataaccg ttagttttgg
     3001 caataaggct tgccacacaa tactatgctg taaataacta aaggcttttc tatgcttacg
     3061 gaatatactt ttcagccaac acgtgtcctt gttctctgcc aactttaaat ccagtttctc
     3121 tagtgcctca tcactaaaag tctgcctcac tctctccgcc accaagtcat gacgaatgtg
     3181 cttgcttttg gttagcccta gatcctgcgc taagcgctga taaaacagcg tccactgctc
     3241 aaggcttggg gaaagctctt gcgctcgtgg agcatctaac agaggggcta tataagcagc
     3301 tagtgctgtt aattgagata gggagtcttt ggggtagtct gaaagcagct cagtatgacc
     3361 caaagcccaa aattgatgtc ggtgatcatc tacagctcta tcaaagaaga ctaaagcacc
     3421 gtgttttgga caatatggca aagcgggcaa ataccaatct cgttgccaaa aggcttcccc
     3481 atacctattt agctgaagag caacgcaatc agggcagtat ctaaagcggt tatcgctctt
     3541 aactctagaa gcagcgactc ctagcattaa atgcaccgca ccttgcgctt ggtactccat
     3601 taaccgaata gcttcgtctc ggcgctcctt gcctacaaac ggagcatata aagggaataa
     3661 ggtatgctca taaataagct gctgaactga agtggcacac tgaatttggc cacctgaaca
     3721 gaggtgatat gctcacctca gaacaacaca ggtgctccaa tgaaaaaaag aaattttagc
     3781 gcagagttta aacgcgaatc cgctcaactg gttgttgacc agaaatacac ggtggcagat
     3841 gccgccaaag ctatggatgt tggcctttcc acaatgacaa gatgggtcaa acaactgcgt
     3901 gatgagcgtc agggcaaaac accaaaagcc tctccgataa caccagaaca aatcgaaata
     3961 cgtaagctga ggaaaaagct acaacgcatt gaaatggaga atgaaatatt aaaaaaggct
     4021 accgcgctct tgatgtcaga ctccctgaac agttctcgat aatcgggaaa ctcagagcgc
     4081 attatcctgt ggtcacactc tgccatgtgt tcggggttca tcgcagcagc tacagatact
     4141 ggaaaaaccg tcctgaaaaa ccagacggca gacgggctgt attacgcagt caggtacttg
     4201 agctacatgg catcagccac ggttcggccg gagcaagaag catcgccaca atggcaaccc
     4261 ggagaggcta ccagatggga cgctggcttg ctggcaggct catgaaagag ctggggctgg
     4321 tcagctgtca gcagccgact caccggtata aacgtggtgg tcatgaacat gttgctatcc
     4381 ctaactacct tgaaaggcag ttcgccgtga ccgagccaaa tcaggtgtgg tgcggtgatg
     4441 tgacctatat ctggacgggt aagcgctggg cgtacctcgc cgttgttctc gacctgttcg
     4501 caagaaaacc agtgggctgt gccatgtcgt tctcgccgga cagcaggctc accatgaaag
     4561 cgctggaaat ggcatgggaa acccgtggta agcccggcgg ggtgatgttc cacagcgatc
     4621 agggcagtca ttatacgagc aggcagttcc ggcagttatt gtggcgatac cagatcagac
     4681 agagtatgag ccggcgcgga aactgctggg at
//
LOCUS       DAJTYZ010000215         4599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000215 DAJTYZ010000000
VERSION     DAJTYZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.216"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(30..230)
                     /locus_tag="OO596_003968"
     CDS             complement(30..230)
                     /locus_tag="OO596_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719677.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(230..502)
                     /locus_tag="OO596_003969"
     CDS             complement(230..502)
                     /locus_tag="OO596_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6719678.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(505..783)
                     /locus_tag="OO596_003970"
     CDS             complement(505..783)
                     /locus_tag="OO596_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719679.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(784..1341)
                     /locus_tag="OO596_003971"
     CDS             complement(784..1341)
                     /locus_tag="OO596_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719680.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPGKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            complement(2905..3456)
                     /locus_tag="OO596_003972"
     CDS             complement(2905..3456)
                     /locus_tag="OO596_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6719681.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            4255..4551
                     /locus_tag="OO596_003973"
     CDS             4255..4551
                     /locus_tag="OO596_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6719682.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT         1294 a          941 c          879 g         1485 t
ORIGIN      
        1 gtttttctaa tttctcttaa agtctcggac taccaataac ctaattccgc tttatttcgt
       61 tcaaaccatt ccgctgtaac tgctgtcttt agtgttgcgt ttgggctggt ggcatgaatg
      121 aagatatctt ttggattgca agcatcaccg aaaccacctt ctttgatgag caggtctgat
      181 acatgcttaa tcattttatc tgtcaaatat tcgaatttaa tacataacat taaatctcca
      241 ggagtaaatt gtttgggtgg caatttcttt tattaccatc cctgaagata atctttttag
      301 ttgcctgaac ttcatgatgc aaaaaccaga tgacatactt cagataataa tcacgagtgc
      361 cataacgaat cattggatta ccgtaagcat catctcttgt cttaacctct ccaccatcaa
      421 tccattcaag cattcctgtc tcataattaa tcatgaagta agttgcaaag aaccgtttaa
      481 tatcatctcg ccattgcttc ataatcagac ctcctgagac agtttgtgaa ttttgatact
      541 tagtaacctt gctaaatcca gtccaaaagc atttaaggat ttcagactat caaggtcatc
      601 ttgcttaatc ctgatatggt gtcttgcagc ctctaagcga ctattaagca aatcctgttc
      661 gttaatatca cggttaattg caagcttcat cactgctgag tgtggcagac ctgtaatgtt
      721 tttgattcgg ttcagttcat caaaggctgt ctttgtaact ctggttaatg tgtgtttatt
      781 catttaaaaa tcctctgtaa ctaaataggg gtcaaactcg aaatctggtt gctcgttttc
      841 cattggctca atattcccaa cggcaatatg atgttcaaca atgccaatta atttcgcgca
      901 catcacagca agcaaagcgc aatatttatc gcttggtaat tcgtaactaa gtctggttgc
      961 ctgtcgctga agatgatttg tcacggtgga tgcgttaatt tcgtcaagat tctttagcag
     1021 ataaaatagt tttttgttgt atttcgggtg gccttcactt ctacagaacc caataaaaac
     1081 atcatcaagc ttgcctggta actgctcgta actgtcaaag ttagccagat aatttttaat
     1141 agtattcttt gttgttttgc gtactggcat aaatctgtct cgtggcgtaa tgctacgaat
     1201 gaccgcttca atgttcttac gttcggattt gctaaaggtt gggacttcgt agctggtggg
     1261 gttgtttgtg taagcgtagg agttgtgctt gttgcgttct cttgatgcct gaagcatcgc
     1321 tctaaattct gaatactgca ttcatgttct gactctcata agatgttatt attctgttaa
     1381 cctatataca atattagcat tttcaaagac taatgtcaag tatttttaac attttcgtaa
     1441 catctgtggt ataatgggtt tatggggaga gtaaaatatg aggcgtggct atgtatttgg
     1501 tcgcatattt tgcattgcac acagacctct tcaatcgccc tgtaacgagt taaacgacct
     1561 cttcaggtca actcataagg tcaggtactt tttgtgtcag aatgcgattg agagcgtctt
     1621 acaaggaaaa ctaatggtaa atctccctcc tgagctgggg tcacctgatg tatggactgg
     1681 tggaggtatt gggctgattg caggcctgct acttcgctac gtcctcgtca gagtgcttgt
     1741 tgtctttaaa ctgttcggtc gtacatggac gcttgacctt aaggataacg accacacaga
     1801 cccccgttaa actccttcaa aacccctgca cgagatgaca gatagaagtc tatggtacag
     1861 gggtgtcttt agcattttgt gctatctatt aaaaaaatag cttgacagaa ccggaaagcc
     1921 gtatctatca agcattcacc tctctgacag taatttctaa aagttcttat ttttcaataa
     1981 gttgtaatta gccatatata gagaactaaa agatttttta gctattttgt ttaaaagcat
     2041 atcaaacgat acattttatt gatttaggtc aaagacaaaa gcattgacaa aatggtaaga
     2101 ttgttcacat gaccttactt aacagacttt taagagactt tgaaacagcc tgtttaacag
     2161 gtcactacct ctggtcttat cgttcccttc tttctaggtg gttatctgtt aagagttttt
     2221 cagtttttat tcttaagata attgttaaga tgatactctc ccttcgggtt atatcgtatc
     2281 tcttctcccg ttaaatatcc cttcacagtc agactaaata acacctgtgt agttcttggt
     2341 agaatctttc aaggtaactt agctctgagt ttatgaagtt gacctatgag atagactcta
     2401 ccgaatctgt tagtcaattg ttgcttaggt gctgctaagg cttacttagt ggaaagagag
     2461 gggatatgtg accatagggg aacccctcct gttaaaagac tacttagaag tctgaggttt
     2521 tagtaaaatc tgtgaatatg gcttctcaga cgctctgtaa cgcattctaa gcgattttac
     2581 ccctttattt taaggtggtt agtacatccc tataggttac ccctaaaaat ggggttaaaa
     2641 ctcgctacag agctttctat gagattagct tctaatcgaa tcaactatga ggtttggtag
     2701 gattgtgcaa aatctgtaca gtaacttttc agaatctgaa aatttttata aaaaatgact
     2761 agccctcttt gaatctcatc ttccccgaat cttcaaaaat atctgacaga tgtctgtagc
     2821 tgctaagtac ccgtcacacc agctatgtga cccccttggc aaaaaagcca aatatcagct
     2881 acatccatgt aactattcag taacttattt agcaagtctg taataagttg ctctagagat
     2941 tcctaactct tcctgaatct gcaacctcgt cttaccttgt gaaataagcg tctgtacagc
     3001 ctctgaagaa gcttttacag gcctccccat atgtacccct tcagctttag cacgttcaat
     3061 accagccttt ctacgctctg ctatcaagct tctttcaagc tgtgccaacg ttgacataat
     3121 gcctaacatt gcttcaccca tagcgcttga taagtcaaac ccttctctaa ggctgataac
     3181 tgtcacacct ttagcctgta gcgctttaac cgttgataac acatcaattg tgtcacgtcc
     3241 aagacggtct acagctacca caactacggt gtcaccttct cgtgcataag ctaatagtct
     3301 gctgaaaccg tttcttaaag aagctttgac agaaccactg acagcttcat cagtgaacca
     3361 tttagcaatg ttgtaccctg cctcttcaat ttgcatttgc tggttagtga tggattggtc
     3421 atctgtactc accctgcaat atccaagtat cgccataatg ttccccttag aagtctcaaa
     3481 agttagtctc ataagtttag ctgtctcata atccaaaaac aaccttttga gactacttga
     3541 gggtgcattc taccgttacg ttgaaagtgt ctcaaaagga agtcctttta agactggtta
     3601 tgaaggagct atgaggttga ttaggggttg gtacaagccc aactaaccga atcaactatg
     3661 aagattttag gagggtaact gtaaccatca ctttctaact ttcacgtagg aaattgttaa
     3721 gtggtttgat agttacacca tgtgaccaag catctatata tcgttatata gccctgtata
     3781 tagcactata tgtagtgtcg agagaggaac gactaacacg atacagttta ccctgaaaag
     3841 agacttcaca gattctttat atatttcaat aagttagggt aaaaagggac tataccgaga
     3901 ctgtatagaa tctgtgtaga attgcataga tacttggaaa tctactatta aattgcatag
     3961 ggactcacct gatattgtcc tgtttttaac aggtctccta gaaaaccgaa tagacttcat
     4021 tcaagggtca ttgaagtcat aatcatttca gtagaaaaac caagtctcga ttttaaatgc
     4081 aaatagcaat catattcaat aaggcaagac gatcaggatc agtgaaaatg tcatttttgg
     4141 cgtaattgac cttactttat gcatggtctt aacaaagaca tcatctttcc gttgcagatc
     4201 ttcgcccttt caaataacgt actttacaac tttcccgaac aaggagttgt gcccgtgttg
     4261 tatgttattt acgctcaaga caaagctgat agcctcgaaa aacgcctttc cgttcgtccg
     4321 gcacatttag cacgtttaca gttactgcat gatgaaggtc gtttgttgac agctggtcca
     4381 atgccagcgg tagacagtaa cgatccaggg gctgcgggat ttactggctc aaccgttatt
     4441 gctgaatttg aatcgctgga agcagctcag gcctgggccg atgctgaccc gtacgtagcg
     4501 gcaggtgtct atgaacacgt ttcagtgaaa ccatttaaga aagtgttctg aatagagatt
     4561 caatatttaa gggcacggtt tttgcaaccg tgttcttgt
//
LOCUS       DAJTYZ010000216         4595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000216 DAJTYZ010000000
VERSION     DAJTYZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.217"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(98..856)
                     /locus_tag="OO596_003974"
     CDS             complement(98..856)
                     /locus_tag="OO596_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6719683.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(864..1967)
                     /locus_tag="OO596_003975"
     CDS             complement(864..1967)
                     /locus_tag="OO596_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6719684.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWQINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1977..3158)
                     /locus_tag="OO596_003976"
                     /pseudo
     CDS             complement(1977..3158)
                     /locus_tag="OO596_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3226..4251)
                     /locus_tag="OO596_003977"
     CDS             complement(3226..4251)
                     /locus_tag="OO596_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6719685.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1269 a         1190 c         1085 g         1051 t
ORIGIN      
        1 gggtctcccc atgcgagagt agggaactgc cagacatcaa atcaagcgaa aggccatccg
       61 aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc tgcgataaaa
      121 atgccttcgt acgctctgat ttaggatgcg caaaaaattc atcaggtgca gcttgctcca
      181 ctatttcccc acgatccata aaaatcaccc ggtcagcgac ggttcgcgca aaccccatct
      241 catgcgttac acacaacatt gtcatacccg attgcgccag cccaatcatc gtatccagca
      301 cctctttcac catctccgga tcgagcgccg acgttggctc atcaaacaac ataattttcg
      361 gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacca gaaatttgtc
      421 cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc atcgccagag
      481 cttcagcctc tttctttggc atcttgcgta cccaaatcgg tgccagggta cagttctgta
      541 gaacggtcag atgagggaag agattgaaat gctgaaagac cattcccact tcctgcctga
      601 cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctactacg atccgtccct
      661 gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg gaaccagaag
      721 ggccacacag aacgatccgt tctcccagtt gcacggttaa atttatattt ttcaaaacat
      781 ggaattgtcc ataccattta ttgacgtttt ccagcgtaat catcgcgtta gcaggttgca
      841 gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa acgtttttcc
      901 agatgctggc tatagcgcga catgctgaaa cagaagatcc agtagatcag tgcggcgaaa
      961 acataccctt ccgtcgacat acccaaccag gcgggatcaa cggttgcctg ctgaacgcta
     1021 ctgaaaagat cgaacaaccc gataatgatc accaggctgg tatctttgaa gagtgcgatg
     1081 atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat aaccagcccc
     1141 tgagttttcc agtaacccaa tgccagtgac tctgccgctt catattgccc tttaggcagc
     1201 gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag gatcacgcca
     1261 accagcgcgc ggatcagttt gtcgatattg gtgccttctg ccataaacaa aggcagcatg
     1321 accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc gataaaaatg
     1381 accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag taagatcccc
     1441 cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt cagcccgccc
     1501 cattgccggg tttcaacccg ctccagacca aaaaaaccgc catacatcag ccaccagaca
     1561 atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg gagtattttc
     1621 cagaacattg gtgcgattga cacaaggcca atcagtaatg ccaggttaat ttgccagcgt
     1681 tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat gaagacccaa
     1741 caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccaattagc ctgcaaaaat
     1801 gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc aatagtcagc
     1861 aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc gcggcttgag
     1921 ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat gactccttag
     1981 cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat agtcaggctg
     2041 ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc cgtctggttc
     2101 agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc ggcaagagag
     2161 gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac tcgcagtgcc
     2221 tggggaataa tgacctagcg tagcgtaacc gggttgggta atcccagtga ccgagccgct
     2281 tcatgttgac cataaggcac tgcctggatc ccagcgcgga taatctcggc gataaatgca
     2341 gatgtataaa ccgaaagtgc cagcgttaag gctgccagtt caggaattaa aaccatcccg
     2401 ccgcggaagt taaagcctcg tagggctggg acatcccagt gtagtgctgc gccaaaaagc
     2461 cattgcgcca gtaaaggcaa accaatgatc aacactgcgg cgatcggcca ggtgcggcgc
     2521 agttgtccgg tctttatctg gtatgtttta ttaaagcgga atagcccaac agaaaggact
     2581 atagccataa caacagccag gataaacgca ataaatccgt ctcccagctg cggtgacgga
     2641 atataaagcc cacgattgct caaaaaggcc agatcaaacg cgctcacagc ctggcgtggt
     2701 ccgggcaaat tgcgcaacac ggcaaagtac cagaagaaga tttgcagcag cgggggaata
     2761 ttacggaaga tctcaatata aattgtggaa agctttcgta gcagccagtt atccgaaagt
     2821 ctcgccagac cgataaagaa gcccaaaaca gaagcgaaca caatacacaa tgcagaaacc
     2881 agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc ctgctggtaa
     2941 tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc aaaacctgaa
     3001 gtaatgccac gattattgag attcgttaca gtgttgtgaa ataaccaacc aacaatgccg
     3061 acaacagcaa caacggcaag gatctggaat aaccaggcac gaaccgtagg gttggcaaaa
     3121 gagagtgagc ctttaacggt tgagcggcga tgagacatag aaacctcgga aagcataacg
     3181 ctgaagagag cgccgccgta gcgacgctcg ttacctcaca ccgacttaac gcacgggcgg
     3241 cgcgtactga ataccgccgt tattccagag attattttgc ccacgtttaa ttttcagcgg
     3301 gctttctgaa cctacgttac gctcgaaaat ttccgagtag ttacccacct gtttgatgat
     3361 gttataggcc catttattat ccagcttcag atccttgccg taatcgcctt ctttacccag
     3421 cagatgtgcc atatcaggcg ttgctggatt agccgctttt tcatcgacgt tctgggaatt
     3481 gatgcccatc tcttcagcat tcagcatggc gaaaagcgtc cagcgtacaa tcgagaacca
     3541 ttcatcatcg ccacgacgaa ctaccggccc aagcggttct ttagagataa cctccggtaa
     3601 gacaatccat tcagctgggt tgcttaattt gatgcgcagg gcatacagtt gcgactgatc
     3661 cgaggccagc gtatcgcagc gaccagattc cagtgccttc gctgattcgt cagagcgatc
     3721 gaaagtcacc ggtgtgtact tcatattgtt tgccttgaaa tagtcggcga cgttgagttc
     3781 ggtatctgta cccgcctgaa tacagacggt agcgccatcc agttctttcg cgctttttag
     3841 ccccgcttta tcgtgcgtca ggaagccaat gccgtcgtaa tcagtgacgc cagtaaatgc
     3901 cattcccatc ccggcatcgc gagatgaagt ccaggtcgta ttacgggaga gcaaatccac
     3961 ctcccctgac tgtaaagcgg tgaagcgttc ttttgcagtg agcggggtat atttcacttt
     4021 cgtgtcgtca ccaaatacag cagcggcaac accacgacaa acatctacat caatacctga
     4081 aaacttaccg tctgcatcgg cataagagaa cccaggtaat ccatcactga tcccgcattg
     4141 cacaaaacct tttttctgta cggcatcaag cgtcgcgcca gcatgcgcct gatttgcaac
     4201 ggcaagcagc acgctggcgg cagccagtgt ggctatcatc atctttttca taatgcttcc
     4261 tgtgaggcga aaattatcgt tataggacgt ttgggaaacg tcttttacct gtctgtggcc
     4321 atcctcttat aagcaaaagt agtgccatct tttgcgcacg atttcgcgtc gcaataagct
     4381 gagtgtagac aggaaataat aaaaacagcg ccccgcaatg gtgcgaaaat gaaacatgcg
     4441 ccacattttg gcgcaaaata tttacgcgtg ttttactaag cagaataatg attgttagcg
     4501 tgagtatatt tctggtggga ggtgtgaaag ttaaattgat gcgttatttc gaaagcagcg
     4561 aaataacgcg atataacatg ctaaggtaat gactc
//
LOCUS       DAJTYZ010000217         4583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000217 DAJTYZ010000000
VERSION     DAJTYZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.218"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..156)
                     /locus_tag="OO596_003978"
     CDS             complement(<1..156)
                     /locus_tag="OO596_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6719686.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIY"
     gene            complement(156..1076)
                     /locus_tag="OO596_003979"
     CDS             complement(156..1076)
                     /locus_tag="OO596_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6719687.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1214..2113
                     /gene="pgrR"
                     /locus_tag="OO596_003980"
     CDS             1214..2113
                     /gene="pgrR"
                     /locus_tag="OO596_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6719688.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2450..4063
                     /gene="mppA"
                     /locus_tag="OO596_003981"
     CDS             2450..4063
                     /gene="mppA"
                     /locus_tag="OO596_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6719689.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4114..>4583)
                     /gene="ynaI"
                     /locus_tag="OO596_003982"
     CDS             complement(4114..>4583)
                     /gene="ynaI"
                     /locus_tag="OO596_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6719690.1"
                     /translation="DRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRM
                     TNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMV
                     YCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT         1210 a         1024 c         1093 g         1256 t
ORIGIN      
        1 atagatattg ccagacgtct gctccttaca actgccaaaa ggatggacgc tgataatcgc
       61 cggatactgt tttgactcat caaaatttgg tggaaactga atattggcag caatatgcca
      121 ggccatatca ctattgcgga tttgaatatg tttcattaca gagtttcctc aaagaacgga
      181 gctaatactg aaattgcttc agccacatag gctttgccgt cataaagatc catatggttt
      241 gcgccttcaa caatgtgata gcgtttatct tggcttgatg ctcgatcgta caggtcgtca
      301 ctcatccatt tgctccctgc ctggctgccc gccacaatct gcatcggctg agtcaggtac
      361 acttccgcca tatggtaagc atcaaaggta ataatctggt taaggctgcg caaagtagcg
      421 taacccggtg ctgttggata ctgcgcgcga ggggtgtggt aatactccca ggcctgacgc
      481 agttcttcgt tcggcgcatc ggactccttc attggtgcca gtggcataac ggcatattct
      541 ccgctgctaa tatcgctggt tcgggcgttt gaacccgctt caacgtatgg cagggcatca
      601 atagatttca cattgttttc ccaaccatta cggaacatcg aaccaatatt gaccgcacta
      661 acggtaccga tggccttgat gcggcgatcc tgaattgcag cattggctgt atatcctgca
      721 ccggcacaaa ttcccatcgc accaattcgg gtattgtcga cataagaaag cgttgtcagg
      781 taatcaatca cggcactgat gtcttcagta cgaatgtatg ggttttctaa ctgacgcggc
      841 tcgccgccac tttcaccttg ataagatgcg tcataagcaa tagtgacaaa tcccttttcc
      901 gccagttttt tggcataggt tccggccgtt tgttctttaa cgcccccacc tgggtgagat
      961 accacaattg cctgatactg acgggtttca tcaaattttg gagggaaata gatcactgca
     1021 gacaaagaga tggttggatt attgctgtta gtgaagctga ctttattatt catcattccg
     1081 ttcctcatgg agttgtcggt tcgttttaac ggttggtgat atcactatag atattgatca
     1141 ttaagttgat tagacccaaa attatgatta gacctatcat ttaaatgatt aatagattgc
     1201 atggagatac tgaatgaagc gtgaagaaat cgctgatctg atggcgtttg tcgtcgttgc
     1261 agaggagcgt agcttcactc gtgcagcagc ccgcctgagc atggcgcagt cagctttaag
     1321 ccagatagtg cgtcgtatag aagaacgatt gggattgcgg cttctgacgc gaaccacgcg
     1381 cagcgttgtt ccaactgaag cgggcgagca tcttttgtct gttcttggcc ctatgttgca
     1441 tgacatagat tcagccctgg catccctgag cgagctgcag aaccgcccat ccgggacaat
     1501 acgtattact actgtagaac atgcagccaa aacgatattg ttaccagcaa tgcgcacatt
     1561 cctgaaatcg catcctgaaa ttgatattca gctcaccatt gattatggtt tgaccgatgt
     1621 cgtttctgaa cgttttgatg caggcgtccg tctgggtggg gagatggata aagatatgat
     1681 cgccattcga atcgggccag atataccaat ggctattgtt ggctcaccgg attatttttc
     1741 tcgccgaagt gttccaacgt cagtgtcaca attaatagat catcaggcaa ttaatttgta
     1801 tcttcccaca tcgggtacag caaatcgctg gagattaata cgcggtggac gtgaagttcg
     1861 tgttcgcatg gaaggtcagc ttttactgaa tacgatagac ctgatcattg atgctgcaat
     1921 tgatgggcat ggattggcgt atctacctta tgatcaggtt gagcgggcta ttaaagaaaa
     1981 aaaactgata cgtgttttgg ataaattcac accagattta cccggttatc acctgtacta
     2041 tccacaccgt cgacatgctg gctcggcatt ctcattattt atagataggc tgaagtataa
     2101 aggtgctgtt tagcactact tgctgataca ttaatttaat ttttctctta acgtattctc
     2161 agttcctttc aacgttttgg ttatttttta ttcttcgtac aatggcgaca gatgctgatt
     2221 atgataccga aaactggttt gaacgtgcga agcctgaacg tagtgttcgg tgttctatgt
     2281 gctttaccgc attttgtcgt ctattattta cactaaatct gatttaatat attgatacat
     2341 aaaacatttt atgctccctg tgtcactttt ttgatggaag ttgtttgcat ttctttaagg
     2401 cgaaacaaat aattacgcat caattttaat gtcggttaga gggaaactta tgaagcactc
     2461 tgtttcagtc acgtgttgtg cgctgttggt cagcagcatt tctctttcgt atgctgcaga
     2521 agttccgagc ggcacagtac tggcagagaa gcaggagctg gtgcgccaca ttaaagatga
     2581 gcctgcgtcg ctggatcccg ctaaagccgt gggcctgcca gagattcagg tcattcgcga
     2641 tctgtttgaa ggactggtga atcagaacga gaaaggggag attgtccccg gcgttgcgac
     2701 tcagtggaaa agtaatgaca accgtatctg gacttttacc ctgcgcgata acgcaaaatg
     2761 ggcggatggc acaccggtaa cggcgcaaga ttttgtctac agctggcagc gtctggtgga
     2821 cccgaaaacg ctatcgccat ttgcatggtt tgccgcgctg gcgggaatca acaacgcaca
     2881 ggcgattatt gatggtaaag ctacgccaga ccagcttggc gtcaccgcag ttgatgccca
     2941 tactttgaaa attcagcttg ataaaccgtt gccgtggttt gtgaatttaa ccgctaactt
     3001 tgccttcttc ccggtgcaaa aagccaacgt agaaagcggt aaagagtgga cgaaacccgg
     3061 aaatctgatc ggcaatggcg cttatgttct taaagatcgc gtagtcaatg aaaaactggt
     3121 cgtggtaccg aatacccatt attgggataa cgccaaaacg gtactgcaaa aagtgacctt
     3181 cctgccaatt aatcaggaat ccgcagccac taagcgttac ctcgcggggg atattgatat
     3241 caccgaatcc ttcccgaaaa atatgtatca gaagctgttg aaggatattc cggggcaggt
     3301 ttatacgccg ccgcagctcg ggacctatta ttatgcgttt aacacgcaaa aagggccgac
     3361 ggcagatcag cgcgttcgtc tggcattaag tatgacgata gatcgccgcc tgatgaccga
     3421 aaaagtatta gggacgggcg aaaagccagc gtggcatttt acaccagatg ttaccgccgg
     3481 atttacgccg gaaccttcgc cgtttgaaca aatgagtcag gaagaactga atgcgcaggc
     3541 aaaaactttg ttgagcgcag ctggttatgg tccgcaaaaa ccgctgaagc tgacgctttt
     3601 gtataacact tcagaaaacc atcaaaaaat tgcgattgct gtagcatcga tgtggaaaaa
     3661 gaaccttggc gtagatgtta aattgcaaaa tcaggaatgg aaaacctata tcgatagccg
     3721 taacaccggc aattttgatg ttatccgcgc ctcatgggtg ggggattata atgaaccctc
     3781 cactttcctg acattattaa cgtcaacgca ttcaggaaat atttcacgct ttaacaatcc
     3841 ggcatacgac aaagttctgg cccaggcatc gacggaaaat accgttaaag cgcgtaatgc
     3901 cgattacaac gcggcagaaa aaatcctcat ggagcaagca ccgattgcac caatttatca
     3961 atataccaat ggacgattaa tcaagccgtg gctgaaaggt tatcccatta ataacccgga
     4021 agatgtggcg tacagccgga ctatgtatat tgtgaagcat tgatgtgatg ggaactggcg
     4081 ttacccttgt ggataacgcc agtgatgtct gatttagcgc ccctgttccg gcggtgtaat
     4141 gttatccata tacagcgtct ggctggggaa ggcaaagtcc gcgccgtgtg actgcacaat
     4201 atcgataatc ttcaaataaa cgtcttgctg tgcagcaagc cattcagccc ataccgtggt
     4261 tttggtaaag caataaacca taatattcaa tgacgagtcg gcaaactggt tgaaataaac
     4321 cagtaaggtt tgtcgctggt cgatggccgg gtgatttttc agcatctcac gtacggcttc
     4381 gacaataacg cccacttttg ccgcatcctc ataacgtaaa ccaatggtcg tggtaatgcg
     4441 gcggttggtc attcgtcctg ggttttctac gctgatcgac gaaaacagcg agttcggtac
     4501 gtacaatgga cgattatcaa aggtcgtaat tttggtaatt cgccagccaa tttccgctac
     4561 tgtaccttcg atatttctgt ccg
//
LOCUS       DAJTYZ010000218         4429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000218 DAJTYZ010000000
VERSION     DAJTYZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.220"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..226)
                     /locus_tag="OO596_003983"
     CDS             complement(<1..226)
                     /locus_tag="OO596_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719691.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILS"
     gene            455..1063
                     /locus_tag="OO596_003984"
     CDS             455..1063
                     /locus_tag="OO596_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6719692.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1161..2552
                     /gene="selA"
                     /locus_tag="OO596_003985"
     CDS             1161..2552
                     /gene="selA"
                     /locus_tag="OO596_003985"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6719693.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2549..4393
                     /gene="selB"
                     /locus_tag="OO596_003986"
     CDS             2549..4393
                     /gene="selB"
                     /locus_tag="OO596_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6719694.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          997 a         1128 c         1307 g          997 t
ORIGIN      
        1 gggagaggat gaacggcagc ggggcgggca gggcgatgtc ggtttcaccg ggaaggacct
       61 ttgcaccgag caggggattg accggatggc cggacgtcac tccgccgggg cacaccgaac
      121 aggccacacc ggtgggggca ccgatgcgca ccccggctga accctgaaca atgctaccgc
      181 catactgcgt catgtcgccc tgacgcgctg ccggttttcc gctcatctgc tcatccttgt
      241 tttatcgggt tatttgtctg cttccaggca aaaccctaca gatttataag aaagccttaa
      301 gtgagatatc catcagatag aaacaattag gaaaatattt atatcaccat gataaatatg
      361 tgatttattt ggaagtaaca aaccatcaac attttctgcc ctttttccca tcaccaggcc
      421 atactgatat ttttcctcgc acaaaggact cggcatgaaa ctcgtcggta gctacaccag
      481 cccgtttgta cgcaaacttt ctattctgtt gttagaaaag ggcataactt tcgaatttat
      541 taatgaactg ccctataacg cggacaacgg cgtggcgcaa tttaacccgt taggaaaagt
      601 gccggtgctg gtgaccgaag agggcgaatg ctggtttgat tcgccgatca tcgctgaata
      661 tattgagtta atgaatgtcg ctccggcgat gttgccgcgc gatccgctgg agtcgttgcg
      721 ggtgcgcaaa attgaggcgc tggcggatgg cattatggat gccgggctgg tatcggtgcg
      781 tgaacaggcg cgtccggcgg cgcagcagtc tgaagatgaa ttgttacgcc agcgggagaa
      841 aatcaaccgc agtctggatg tgctggaagg atatctggtc gatggcacac tcaaaaccga
      901 tacggtcaat ctggcgacta tcgccattgc ctgtgctgtc ggatatctca atttccgccg
      961 cgttgcgcca ggttggtgtg tcgaccgccc acacttagtc aaactggtcg aaaacctgtt
     1021 tagccgcgaa agttttgctc gcaccgaacc gccaaaggct tgatgcgcga tatgtcctcc
     1081 tgacccatct cacgttacaa tccgtggtta tgttaaacgc ccttctccgt gtgagagggc
     1141 cttgatcagc caggtttcct atgacaaccg aaacgcgttc cctctatagt caacttccgg
     1201 ctattgatcg cttattgcgc gatagctcct tcctttcttt gcgtgatact tatggtcaca
     1261 cccgcgtggt ggaattgttg cgtcagatgc tcgacgaagc gcgagaagtg attcgtgaca
     1321 gccagacgct tcctgcgtgg tgtgaaaact gggcgcaaga agtcgatgcc cggttgacga
     1381 aagaggcgca gagcgcgctg cgtccggtga tcaacctgac gggaaccgtg ctgcatacca
     1441 accttggacg agctttacag gcggaagccg cggtggaagc cgttgcgcag gctatgcgtt
     1501 cgccagtgac cctcgagtac gatctggacg acgccggacg cggacatcgc gatcgggcgc
     1561 tggcgcaact gctgtgccgt attacggggg cggaagatgc ctgtatcgtc aataacaatg
     1621 cggcggcggt gttattgatg ttggcggcca ctgccagcgg gaaagaggtg gtagtatctc
     1681 gcggcgaatt ggtggagatt ggcggcgcgt ttcgtattcc cgatgttatg cgtcaggcag
     1741 gctgcaccct acacgaagta gggaccacca accgcacgca cgcgaatgat tatcgtcagg
     1801 cggtgaatga aaataccgca ctgttgatga aagtacatac cagtaactac agcattcagg
     1861 ggttcaccaa agcgatagat gaagcggaac tggtggcgct cggcaaagag ctggatgttc
     1921 ccgtagtgac tgatttaggc agtggctcgc tggtcgatct tagccagtac ggtttgccga
     1981 aagagccaat gccgcaggag ttgattgcgg cgggcgtcag tctggtgagc ttctccggcg
     2041 acaagttgtt aggcgggccg caggcaggga ttatcgttgg taaaaaagag atgatcgccc
     2101 gcctgcaaag ccacccgctg aagcgtgcat tacgcgcgga taaaatgacc ctcgcggcgc
     2161 tggaagccac gttgcgtctt tatttacacc ctgaagctct gagtgaaaaa ttaccgaccc
     2221 tgcgcctgct tacccgcagc gcagaggtca ttcaaatcca ggcacaacgt ttacaggctc
     2281 cccttgccgc acattacggc gcggagtttg cggtacaggt tatgccatgt ctttcgcaga
     2341 ttggcagtgg ttcgctgccg gttgatcgcc tgccgagcgc ggcattaacg tttacacccc
     2401 atgatggacg cggtagccac cttgagtcat tagccgcccg ctggcgtgaa ttgccagtgc
     2461 cgatgattgg tcgtatttat gacggacgat tgtggctgga tttacgctgc cttgaagatg
     2521 agcaacggtt tttggagatg ttgttgaaat gattattgcg actgccggac acgttgacca
     2581 cggcaaaaca accttattgc aggtgattac tggcgtaaat gctgaccgtc tgccggaaga
     2641 aaaaaagcgc ggcatgacca tcgatctcgg ctatgcctac tggccgcagc cggatggtcg
     2701 cgtgcctagt tttatcgacg tacccggtca tgaaaagttt ctttccaaca tgctggcggg
     2761 cgttggtggt atcgatcacg cgctgttggt ggtggcgtgc gatgacggcg tgatggcgca
     2821 gacccgtgag catctggcga ttttgcagct gaccggtaac ccgatgctga cagtggcgct
     2881 gaccaaagcc gatcgcgtgg acgaagcgcg tgttgatgag gttgaacgcc aggtaaagga
     2941 ggtgctgcgg gaatacggtt ttgctgaggc aaaactgttt atcaccgcag caaccgaagg
     3001 tcggggaatt gatgccctgc gcgagcatct gcttcagtta ccaggacgtg aacacgccag
     3061 ccaacatagt gtccgcctcg cgatcgaccg cgcatttacc gtaaaaggtg ccggtctggt
     3121 cgtcaccggt acggcgttaa gcggggaagt gaaggtaggc gattcactct ggctgactgg
     3181 tgtaaataaa ccgatgcgtg tacgtgcgct gcatgcacaa aaccagccaa cagaaaccgc
     3241 ccatgccggg cagcgtatcg cgcttaacat cgcgggtgat gcggaaaaag agcagattaa
     3301 ccgtggcgac tggctgcttg ccgatgcgcc gccagagccg ttcacacggg tgattgtcga
     3361 gcttcaaacc catacgccgc tgacccagtg gcagccgctg catattcacc acgccgccag
     3421 ccacgttacg ggacgcgttt cgctgctgga agataacctt gccgagctgg tgttcgacac
     3481 gccgctgtgg ctggcggata acgatcgctt agtgctgcgc gatatctcgg cccgcaacac
     3541 gctggcgggg gcgcgtgtgg tgatgcttaa cccgccgcgt cgcggtaaac gtaagccgga
     3601 atatctgcaa tggctggcgt ctcttgcacg ggcgcagagc gatgccgatg cgttatctgt
     3661 tcatctggaa cgcggcgcgg ttaaccttgc ggatttcgcc tgggcgcgcc agctcaacgg
     3721 cgaagggatg cgcgaattgc tgcaacagcc tggctatatt caggctggtt atagcttgtt
     3781 gaatgcgccg gttgccgccc gctggcagcg gaaaattctc gacacattag cgacttatca
     3841 tgagcaacat cgcgatgaac ctggtcctgg gcgcgaacgt ctgcgacgta tggcgttgcc
     3901 aatggaagat gaagcgctgg tactgttgct gattgaaaag atgcgcgaaa gcggcgacat
     3961 cctcagtcat cacggttggc tgcatctgcc ggatcacaaa gcgggcttca gcgaagagca
     4021 gcaggccatc tggcaaaaag cagagccgct gtttggtgac gagccgtggt gggtgcgtga
     4081 tctggcaaaa gagacgggta ccgacgagca ggcaatgcgc ctgacgctac gccaggcggc
     4141 gcagcaagga ataattaccg cgatcgtcaa agatcgttat taccgtaacg atcggattgt
     4201 cgaatttgcc aatatgatcc gcgatctcga tcaggagtgt ggttcaacct gcgcggcgga
     4261 tttccgcgat cgcttaggcg taggccgaaa gctggcaatt cagattctgg aatattttga
     4321 ccgcattggc tttacgcgtc gtcgtggaaa tgatcattta ttgcgcgacg cattattatt
     4381 tccggaaaaa taaggaaatg attaattaat ttttaaaata aattaatac
//
LOCUS       DAJTYZ010000219         4404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000219 DAJTYZ010000000
VERSION     DAJTYZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.221"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(139..3654)
                     /locus_tag="OO596_003987"
     CDS             complement(139..3654)
                     /locus_tag="OO596_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6719695.1"
                     /translation="MDKNALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4404)
                     /locus_tag="OO596_003988"
     CDS             complement(4106..>4404)
                     /locus_tag="OO596_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6719696.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1141 a         1128 c          905 g         1230 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acag
//
LOCUS       DAJTYZ010000220         4402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000220 DAJTYZ010000000
VERSION     DAJTYZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.222"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(163..2112)
                     /locus_tag="OO596_003989"
     CDS             complement(163..2112)
                     /locus_tag="OO596_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6719697.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2197..2532)
                     /locus_tag="OO596_003990"
     CDS             complement(2197..2532)
                     /locus_tag="OO596_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6719698.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2532..2888)
                     /locus_tag="OO596_003991"
     CDS             complement(2532..2888)
                     /locus_tag="OO596_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6719699.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2888..4384)
                     /locus_tag="OO596_003992"
     CDS             complement(2888..4384)
                     /locus_tag="OO596_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6719700.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1016 a         1314 c          969 g         1103 t
ORIGIN      
        1 ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca gcaagaggtt
       61 aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt gttagcatgt
      121 tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt taaatggaga
      181 atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca aatcgataac
      241 gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg ttgccggtga
      301 agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt agcgtttatc
      361 gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat agacaaaatc
      421 atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac tgttcagtgc
      481 atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct gcgccatcat
      541 gcccagttca agcaatccac caatcagact gatctttccg aggcccttta atttcagccc
      601 tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac gaatacttgc
      661 caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta ctgcctgttc
      721 ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt tcacaaccgg
      781 accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa gcgcaattaa
      841 tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc gtgtcaatgt
      901 gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg tttttacgac
      961 ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca tctcttttat
     1021 gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg cattgatctg
     1081 gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat attctttctg
     1141 cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc gcaacaaatc
     1201 cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca gcgctttcag
     1261 cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt gcagcatggc
     1321 cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc gcaacgagcg
     1381 gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca acataaaatt
     1441 ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg ttgcccccat
     1501 tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa tacgggtcac
     1561 aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt taatcttatc
     1621 cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact gcgccatcat
     1681 ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg catcgtcagt
     1741 aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca caatttcggc
     1801 gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct ccgtcattgc
     1861 cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg ccatttttga
     1921 ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg cggttccggc
     1981 acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca gctggcgctg
     2041 cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct tcagctgaaa
     2101 actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc agttctgtaa
     2161 actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac caggcaacat
     2221 tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac gcaggatctg
     2281 catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac gcgggatccc
     2341 cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac tgccttcacc
     2401 ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct cgcgcaactc
     2461 cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta tttctgtaac
     2521 gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc tgccgtgcct
     2581 tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac atacaccatg
     2641 ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt gtcgaccgga
     2701 aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt ctccttgtaa
     2761 tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat cggcacttca
     2821 atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt tccggcgatc
     2881 tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc accgcaaaga
     2941 cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc gggttatctg
     3001 catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca ataccggcac
     3061 gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga gtgacaatcg
     3121 cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg tacttgcttg
     3181 tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt tcactgtcca
     3241 gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg gaacgctgga
     3301 tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa agggtctgct
     3361 gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc agctcccccg
     3421 tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca aggcgactgg
     3481 caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc gtgatatgtt
     3541 gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt tttgcggtat
     3601 agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca ttcatttcgg
     3661 tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag tcgaatgcct
     3721 catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg gcagcaccga
     3781 cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa ccataataat
     3841 tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc agcgtcacca
     3901 cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc gcttccttaa
     3961 tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt acacgggagc
     4021 gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc gtcaccctga
     4081 ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca ccgaaaggat
     4141 ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca caaatctggc
     4201 gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct gcatcgttgc
     4261 tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc gcagaattgt
     4321 tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta aaacttatcg
     4381 tcatgatttt ttagccttat cc
//
LOCUS       DAJTYZ010000221         4327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000221 DAJTYZ010000000
VERSION     DAJTYZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.223"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..581)
                     /locus_tag="OO596_003993"
     CDS             complement(<1..581)
                     /locus_tag="OO596_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6719701.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYTSYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(644..1093)
                     /locus_tag="OO596_003994"
     CDS             complement(644..1093)
                     /locus_tag="OO596_003994"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6719702.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1090..4182)
                     /gene="ebgA"
                     /locus_tag="OO596_003995"
                     /pseudo
     CDS             complement(1090..4182)
                     /gene="ebgA"
                     /locus_tag="OO596_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1029 a         1089 c         1167 g         1042 t
ORIGIN      
        1 aagttcggga tcatcgcgtc aacggtgatg gcgtcagcag gctgtacgcc gccaaccagc
       61 gccgtacctg ccagtaaaat gtaggagagc gttaacagca gcatcagcgt tgaagtgacg
      121 gaggtaattg gccccaacat tttcgccccg ttggtggaaa cccaggtgga gaaggcgaac
      181 agcaccatac tgataatggt ggtggcaacc ggcgtcataa tatattcgta tccgaggaag
      241 gcatacgaag tataagcaat aacgcgcggc aacagtgagg tgaaaaagaa caggtttacg
      301 aaccagtagg tataggcagt aataaatgcc caacgtccgc ccagcgaact ttttacccat
      361 gcgtagacac cggcttctga gtttttattt aacgaaacaa attctgcgat gatcagacag
      421 aagggaataa aataaaaaat cgtcgcgagg aaaaacatcg gtgccgaggc cagtccaaga
      481 tcaatattat tattgataac gttgttaaag ctgtaaacgg cggcaaaagt cagcgagagc
      541 aagccgaatt tgccgattgt attacgtttg gtatcagaca taacacatct ccgaaaatga
      601 gtagggtagc ggtggcggca aactcctttt gccgccgtag ttgttatttg ttatggaaat
      661 aaccatcttc gatggtgact ttgagaacca cttttttgac cgcgttattg cagataaacc
      721 gatacgcttc atggatatcg caaataacga tttgcccttc gtggacctca acggtttctc
      781 cgcagccttt taaatattca cggtcagttt catcacgata atattccact acctgtaatg
      841 tctctttcgg cgcatattca attttttgct gcccttgcag gtagtaatgc acttcaaaat
      901 agcgtcgatg cccggtaaat agcgcatcgg tcgcggagtc tgtctccacg cggtaagtca
      961 atgagtcacc gatggagtgg gcgacgccag gctgaatgtt gtcgatattt tcaatcgctt
     1021 caacgcagcg ttgccacttc ttgccagagg cgtaaatctg gcggaactgt tctaagttat
     1081 cgatgatcct cattgcttat tctcgctgtg caaattcgtg gaaaagaacc ctgcgccgaa
     1141 ctcatacgac gccaggcttt gcgcggtagc ttctccgcca gaaaccggca gcaacgtaaa
     1201 gccgtagctg aagtcacgga accagacgcg ccaggagtcc agcacctcgc tgccccagga
     1261 gttggagccg aggccaagca gctggtgatc gaggttcagg gtgatgtcat cactgcgctg
     1321 cagctcgtta cagtgctggg cagcgtggat gttttcctag gtatagtgcc aggcgctgaa
     1381 gttaattggg cgctgcggaa ccaccagcag accgttaccg tggcggttag tcagtgccgt
     1441 ccagcggaca tgctgacggt taccgttgtt ctgcgggaag ggatagttct cgaacatggc
     1501 atcgacggtg ctgcgccaga tatcgatgat gttagcctgc tggctgtcgg cgtagttttc
     1561 gcccggtcca cgaccgtaat acgccacctg atcgtattcg ccgttaattc ccatggtgaa
     1621 accgatgcac ggaatgatgt gcggatagtc gccgtaacgc tcgccggaaa gcgccacgtt
     1681 aacctggcca tcggcagcga tgcgccagat gtaggtgcag cgcattccga aatcaaacac
     1741 cggcggggca ataaccgtgc ggctgatgat caacacttca ccctcgctct gttctacggc
     1801 aaagtcgcgc agatgttcct gcatgatctg caaatgattc ggctgccaca acccttcgta
     1861 ctcctgcttg tggttgtcga tcatcggctt gaagaagttg atctttggct cgcgggtcag
     1921 cagcgactcg ccatttacct gccaggatgt cggtttgcca ctcatttttg agaaggtgat
     1981 cgcgaagttg tagccgcgaa cggtgcagct caaacgatcg tcttccagcg tcagcggacg
     2041 cgcattattt ggtgcgaaag gcactggctg cgcggtgttt tccttcagcg ggaactgata
     2101 agtggcgata gaatgcccgg cttcgctgta gcgggtgcgg gaatctttgg tcaccgtaat
     2161 gttgaggaac gcttcgcggg cgtccagctg cggcagcgtg atctgcaagg gggcttcgct
     2221 gttcggcgca acgtcgcgca gtttaatctg ctgcgtcgcg agcgtttcac cttcggcgcg
     2281 cacctctgcg tgcagggggt agtcatcaag cgtggtaaac cacagtttat tttcgacttt
     2341 caactcgccg cgagtcagat cccgcgcgtg gatttttacc ggcgcgataa cctgtttgta
     2401 ctctttcagg cccggtcccg gcgtctgatc ggaatagatc aaaccatcaa gacagaagtt
     2461 atagttgttg ggatagtcgc cgtagtcgcc gccgaattta taccagacat tgccgtggtc
     2521 gtcctgtgcc tggatcccgt ggtcgcacca ctcccagaca taatgaccct gaatgcaatc
     2581 gtgcttatag aagacgttct ggtactccgt cagcccgccc ggtccgttcc ccatcgcatg
     2641 agcatattca cagatgatgc gcggcttcgg atgcgggtat tcaccaaact cattcatcag
     2701 cggcacgcgg gtgtacatgg tggaaataat atcgaccact tcagcatcgc gatcttcttc
     2761 gtaatgcacc agtcgcgtgt catccagcgc cttcgctgcg tggtacatcg cgcggatgtt
     2821 acagccatag ccggattcat tgcccagcga ccagatgatg atcgacggat ggtttttctg
     2881 cgcgtgaata tggcgaacaa tgcgctcgac gtagactttt tcccactgcg gatcgtcggt
     2941 aatacggctg atatcgccga cattagcaaa gccgtgcgat tcgacgtcgg tttccgccat
     3001 cacaaacagg ccgtagatat cacacagttc gtaaaaacgc ggatcgttcg ggtagtgagc
     3061 ggtacgcacg gagttgatgt tgtgctgctt catcaactgg agatctttct cgacgcgatc
     3121 cattccaacg gcgcggcctt tgcgatgatc gttgtcgtga cggttgacgc cgtgcagcat
     3181 cacataacgg ttattgatcc agaacagacc gtcgcgcact ttgatatcac ggaagccaac
     3241 gcgttgtggt accacttcca gaacgttgcc gttggcgtct ttcagcgtca tgaccagatg
     3301 gtaaagataa ggggattctg ctgaccattg ctgcggctgt tcgacagtaa aagcaaagct
     3361 ggcgctggtc agtttttcaa ttgccaaatg atcaatggcg ctgctgtgca ccacgcgttc
     3421 gccatcaaac agggtatatt ccagcgtcgt gacgacaggg gaggcggcga gattttccag
     3481 caccacttcg caggaaagcg tggcatcgca ataggcttcg tcaaagtcgg tacgcacggt
     3541 gaaatcgtta atatgtgtta gttgttttcc gatcagataa acatcgcgga agatccccgc
     3601 tgaccaccac atatcctggt cttccacgta ggtagagtcc gcccattgca tcacgcgcac
     3661 acacaacagg ttgtcgccgg ttttaaccat cgcgctgatg tcaaactctg cggtcaggcg
     3721 actgcccttg ctgaaaccca catactgacc gttaacgtag acttcaaaat aggtttcgac
     3781 gccgtcaaat ttaatcagcg tctgtttacc ctgccagccg tcgctgaggg tgaaaatacg
     3841 ttgataggca ccggttgggt tatcgctggg aacaaacggc acatcgatgg ggaacggaaa
     3901 accttcgtcg gtatattgca gtttgccgtg accttccatt tgccacatgg cggggacggt
     3961 aatatgcccc cagtcagcca ttaactcaga ggtgaaggct tcaggcactt gcagcgggtg
     4021 gtcaaaaaag tggaaattcc actgaccgct taagggcaga aacaggctgc tggtttcgcg
     4081 ggcaaaggta cgcgcttgcg caacagaatc atatgaaaaa aagtacgcac gtggcgcaag
     4141 tcggttttcg tgggtgagct gaatgttttc ccagcgattc atagggcctc ccggtagaga
     4201 gaaatcggaa ccattcatgg tgatggtagt ttgtgtaatt gctggggagt ttagtaaaat
     4261 ttttactaaa aagtgacagg taaatgcatt tactttaact ggatcacgaa aataaacggc
     4321 agcactg
//
LOCUS       DAJTYZ010000222         4302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000222 DAJTYZ010000000
VERSION     DAJTYZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.224"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..2885
                     /locus_tag="OO596_003996"
                     /pseudo
     CDS             1..2885
                     /locus_tag="OO596_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            3866..4302
                     /locus_tag="OO596_003997"
                     /pseudo
     CDS             3866..4302
                     /locus_tag="OO596_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         1092 a         1033 c         1322 g          855 t
ORIGIN      
        1 aggccgccgg tgacctggcc ggggaaatca ccgacgtgac ggacggtgcc gggcgggagt
       61 tccgtctggt gctgaccacg caggcgcagc gtgcggaaga ggcccgcacc tcttcgctat
      121 cttcttctga cagttcccgc cctctctcag cctcagcgtt ccccgacaca ctgcccggta
      181 ccgaatacgg ccccgacagg ggtatccgcc tttcggcggt gtggttgatg cacgacccgg
      241 catacccgga gagcctgccc gctgcgccac tggtgcggta cacgtatacg gaagccggtg
      301 aactgctggc ggtatatgac cgcagcaata cgcaggtgcg cgctttcacg tatgacgcgc
      361 agcacccggg ccggatggtg gcgcaccgtt acgcgggaag gccggagatg cgctaccgct
      421 acgacgatac ggggcgggtg gtggagcaac tgaacccggc agggttaagc taccgctatc
      481 tttatgagca ggaccgcatc accgtcaccg acagcctgaa ccggcgtgag gtgctgcata
      541 cagaaggcgg ggccgggctg aaacgggtgg tgaaaaaaga actggcggac ggcagcgtca
      601 cgcgcagcgg gtatgacgcg gcaggaaggc tcacggcgca gacggacgcg gcgggacggc
      661 ggacagagta cggtctgaat gtggtgtccg gcgatatcac ggacatcacc acaccggacg
      721 ggcgggagac gaaattttac tataacgacg ggaaccagct gacggcggtg gtgtacccgg
      781 acgggctgga gagccgccgg gaatatgatg aaccgggcag gctggtatcg gagacatcgc
      841 gcagcgggga gacagtacgc taccgctacg atgacgcgca cagtgagtta ccggcgacga
      901 caacggatgc gacgggcagc acccggcaga tgacgtggag ccgctacggg cagttgctgg
      961 cgttcactga ctgctcgggc taccagaccc gctatgaata cgaccgcttc ggccagatga
     1021 cggcggttca ccgcgaggaa ggtatcagcc tttaccgcca ctatgacaac cgtggccggt
     1081 taacctcggt gaaagacgca cagggccgtg aaacgcagta tgagtacaac gccgcaggcg
     1141 acctgactgc cgttatcacc ccggacggca accggagcga gacacagtac gatgcgtggg
     1201 gaaaggcggt cagcaccacg cagggcgggc tgacgcgcag tatggagtac gatgctgccg
     1261 gacgtgtcat cagtctgact aacgagaacg gcagtcacag cgacttcagc tacgatgcgc
     1321 tggaccggct ggtacagcag ggcggctttg acgggcggac gcaacgttat cattatgacc
     1381 tgaccggaaa actcacgcag agtgaggatg aggggcttgt caccctctgg cactacgatg
     1441 cgtcggaccg catcacgcac cggacggtga acggcgaccc ggcagagcag tggcagtatg
     1501 acggccacgg ctggctgaga gaaatcagcc acctgagcga aggccaccgt gtcgctgtcc
     1561 actacggcta cgacgataag ggccgcctga cgggcgaacg gcagacggtg gagaacccgg
     1621 agacggggga actgctgtgg cagcatgaga cgaaacacgc ctacaacgaa caggggctgg
     1681 caaaccgcgt cacgccggac agcctgccgc cggtggagtg gctgacctac ggcagcggtt
     1741 acctggcggg catgaagctg ggcgggacgc cgctggtcga gtatacgcgg gacaggctgc
     1801 accgtgagac ggtgcgcacc ctcgcaccgg acctggacat ccgcatcccg tatgccaccg
     1861 acccggcggg aaaccgcctg ccggacccgg agctacaccc ggacagcacg ctcacggcgt
     1921 ggccggataa ccgcatcgca gaggatgcgc actatgttta ccactatgat gaatacggca
     1981 gactgacgga gaagacggac cgcatcccga cgggtgtgat acggacggac gacgagcgga
     2041 cccaccacta cgacagccag caccgcctgg tgttccacac gcggatacag catggcgagc
     2101 cactggtcga gagccgctac ctctacgacc cgctgggacg gcgaatggcg aaacgggtat
     2161 ggcggcggga gcgtgacctg acggggtgga tgtcgctgtc gcgtaaaccg gaggagacat
     2221 ggtacggctg ggacggcgac aggctgacga cggtacagac cgacaccaca cgtatccaga
     2281 cggtatacga gccgggaagc ttcacgccgc tcatccgggt cgagacagag aacggcgagc
     2341 gggagaaagc gcagcgccgc agcctggcgg agaagctgca gcaggaaggg agcgaggacg
     2401 ggcacggcgt ggtatttccg gctgaactgg tgcggctgct ggacagactg gaggaagaaa
     2461 tccgggcaga ccgcgtgagc agtgaaagcc gggtgtggct tgcgcagtgc gggctgacgg
     2521 tggagcaact ggccagacag gtggagccgg aatacacacc agtgcgaaac gttcatcttt
     2581 accactgcga ccatcggggc cagccgctgg cgctcatcag cgaagacggc gatacggtgt
     2641 ggagcggggc gtatgatgag tggggcaacc agctaaatga ggagaacccg catcatgtgt
     2701 atcagccata ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaatc
     2761 gtcaccggta ctatgatccg ttgcagagac ggtacatcac ccaggacccc attgggctga
     2821 aaggtgggtg gaatctgtat cagtatccgc tgaatccggt gagcaggata gatccgttgg
     2881 ggttattctc atgtgagagt tgtagtcatt ttatagatga tataaataat aaactggata
     2941 accttccgtt taacccgaca aaaatgttta cagctagttt aaatactgct aattcagcaa
     3001 ggctgtatgc aagtggcatg acaaaattcg gtataagtgt tgctgccgat ggaacgatca
     3061 ttgctgcccc agtcggtgca gcttctatgg ctttaggggc gtggaatata aaatctgcga
     3121 cagctgcata taacagagca aatttacaat tatcagaatc acttcaggaa aatggtagag
     3181 ataggtgttg gagaaattta ttgggtattt taccttatgg tgagcagttt gatgaccctg
     3241 gtgaaccatc aatattagac gtttatggtg aaaagattga gaatatcaaa gattctccaa
     3301 tgaagttttt tgaagagctt gggacgctgg ggttgtaatg tgtattttta atgatatttt
     3361 tatgtttgtg tttattgtta gtagtatact tgtaaccgtg cattttgtgt attctttatt
     3421 ttttaaaagt ggaaaggtaa aagataaggt aaaaaaatgg attaaagtaa tgtgtgattt
     3481 attttggggg ataggatagt ggtgtgatat ggataaatat aaattacagt ggctggcata
     3541 tttcgacctg acgccggagt agataaaaaa acagacagga ttggagtata gcaagcgtct
     3601 gacaatctct cgggacagta gtatgatgaa gaggcggggt tgtactataa ccgtaaccgg
     3661 tactacgatc cgctaaaggg caagtatatg actcagggaa agatcatcag aagttcactt
     3721 tttgtactaa ataattcgca ttttatgttt aaaaattgag atattcctca ttacctgaag
     3781 ctgttttttt attgcttata cataatcaaa tactccttac ataattaagg agaacaaaat
     3841 ggaaattaaa aaattgatgg aacatttttt gaagcaatat ggtgattttg aaaatggcat
     3901 tcctgttcac gataccattg ccagagttgt atcctgtatc agtcctgcaa aatttcatga
     3961 gtgctttatt aactggatgc gtgactgcca ttcttcagat gataaagacg tcattgcaat
     4021 tgatggaaaa acgctccggc actcttatga taagagtcgt cgcaggggag cgattcatgt
     4081 cattagtgcg ttctcaacaa tgtacagtct ggtcctcggg cagatcaaga cggatgagaa
     4141 atccaatgag attacagcca ttcctgaact tcttaacatg ctggatatta aaggaaaaat
     4201 catcacaact gatgcgatgg gttgccagaa agatattgca gagaagatac aaaaacaggg
     4261 aggtgattat ttattcgctg taaaaggaaa ccaggggcgg ct
//
LOCUS       DAJTYZ010000223         4294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000223 DAJTYZ010000000
VERSION     DAJTYZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.225"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            30..1064
                     /gene="ompC"
                     /locus_tag="OO596_003998"
     CDS             30..1064
                     /gene="ompC"
                     /locus_tag="OO596_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6719703.1"
                     /translation="MAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYAR
                     LGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGRN
                     YGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQYQ
                     GKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQVIYGNNSLNASGQNAE
                     VWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQYQFDFGLRPSVAY
                     LQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDKSDFTKASGVATDD
                     IVAVGMVYQF"
     gene            complement(1253..1636)
                     /gene="quuD"
                     /locus_tag="OO596_003999"
     CDS             complement(1253..1636)
                     /gene="quuD"
                     /locus_tag="OO596_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCR6719704.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1722..1862)
                     /locus_tag="OO596_004000"
     CDS             complement(1722..1862)
                     /locus_tag="OO596_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCR6719705.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1859..2221)
                     /gene="rusA"
                     /locus_tag="OO596_004001"
     CDS             complement(1859..2221)
                     /gene="rusA"
                     /locus_tag="OO596_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCR6719706.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2218..2508)
                     /locus_tag="OO596_004002"
     CDS             complement(2218..2508)
                     /locus_tag="OO596_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6719707.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2501..2671)
                     /locus_tag="OO596_004003"
     CDS             complement(2501..2671)
                     /locus_tag="OO596_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719708.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2671..3126)
                     /locus_tag="OO596_004004"
     CDS             complement(2671..3126)
                     /locus_tag="OO596_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HCR6719709.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3391..3768
                     /locus_tag="OO596_004005"
     CDS             3391..3768
                     /locus_tag="OO596_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719710.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT         1181 a          983 c          924 g         1206 t
ORIGIN      
        1 caatttctgc tgtagctgca tcagtactga tggcgatgtc tgctcaggca gctgaaattt
       61 ataataaaga cagtaacaag ctggatctgt acgggaaagt taatgctaag cactacttct
      121 cctctaatga tgcagatgat ggtgatacta cttatgcccg tcttggcttc aaaggtgaaa
      181 cccaaatcaa cgatcaactg actggtttcg gtcagtggga atatgaattc aaaggcaacc
      241 gcgctgaatc tcaaggttcc tccaaagata aaacccgtct tgccttcgct ggcctgaaat
      301 tcggtgacta cggctccatc gattacggcc gtaactacgg tgtagcatac gacatcggtg
      361 cgtggactga cgtcctgcca gaattcggtg gtgacacttg gactcaaaca gatgtgttca
      421 tgactggtcg caccactggt gttgcaactt atcgtaacaa cgacttcttt ggtctggttg
      481 atggtctgaa ctttgctgct cagtatcagg gtaaaaatga ccgcactgac gtaactgaag
      541 ctaatggtga tggtttcggt ttctccacta cttatgagta tgaaggattc ggtgtaggtg
      601 caacctatgc taaatctgac cgcactaata atcaggttat ctacggtaac aacagcctga
      661 atgcatctgg tcaaaatgct gaagtatggg cagctggtct gaaatatgat gcgaacaaca
      721 tctatctggc taccacctat tctgaaaccc agaacatgac tgtttttggt aataaccata
      781 ttgccaacaa agcacaaaac ttcgaagtag ttgcacaata tcagttcgac ttcggtctgc
      841 gtccgtccgt tgcttacctg caatctaaag gaaaagactt gggtgcgtgg ggtgatcagg
      901 acctggttga atatattgat gtaggtgcaa cctattactt caacaaaaat atgtccactt
      961 ttgttgatta caaaatcaac ctgattgata agagcgattt cacgaaagca tctggcgttg
     1021 ctaccgatga tatcgttgct gtaggtatgg tttaccagtt ctaatttgat tactaaaaga
     1081 tatgttgcgg gaggctttgc ctccccaaca tataagtggc tccctcaagc cacttccttt
     1141 aggagcacaa ccttgcttct aactatataa accttctgtt atatattacc ctttattttt
     1201 gggggcgttg caacgcccca tttttaataa tttttagtaa acaattggca tattaattag
     1261 agttattaac aacgatatcc atctctaacc ggatatctaa tgccattaac atcccttcaa
     1321 ttatgccctc agccttctgt aaccttttcc cgatataacc atcagagcag caatgcttac
     1381 ctgccagtga catgaatgtc ataccgacta cataataatc tactaataaa tcgtgcaaat
     1441 cgctgttgtt ctttttcaga cgggccatgc acccgcaaat aatcatcgcg tcatcgtcac
     1501 aacattgcgg gcgagatttt acttttgaag taattaatcc cttaaaaccg gcggcaatgg
     1561 acgaccaggt cacatcttca tgattattag ccgcccacgc tccccaacgc tcaagaacca
     1621 tctgaatatc acgcatcaac ttactccaca aaaatcagac cagaacgcca attacaagca
     1681 aaaatcaaca aaacagtatt agttgattgt tatctctgac ttcatactcc tgctcctgtc
     1741 agggttttgg cgtaattctt cagtattcgg taatcggtca aaacagaacc ggggaaacga
     1801 tataagcgca gatgccccca gcggtggcga agaagttctg ccatataaaa ctcaaacatc
     1861 attcattccc catttcggtg atggtcagtt ccagcctccc acctttggta acaggcatct
     1921 tcacaacgcg gtaatcaacg acctgagcat catccagcca gaaacctgct ttggtgagtg
     1981 cgtcaaaagc ggctttttgc agattatcca ggtcacggcg acggcgatcc ggcatgtggc
     2041 actcaatacg gattttcact ggcatagcca ggccgatatc cagcattgcg tttttaatga
     2101 ttcgggtgac gttatcgcgg tatgcctgcc cttctgcgct gatgtgtgtg cgcccgcgat
     2161 tatggcggta atagcggtta ttgctcggag gccagggtaa tgtgatgctg taggtattca
     2221 cgccttaata accccctctt tcagccagat aacctgcgtt ctcgccatac cttccagcgc
     2281 gcattctttt gcatactcag catcgacaaa atatgtgcgg cggtcgattt cgtcgtgaca
     2341 ggcagaacat gcaatggtgg caatcaggtc tggcggtttg ataccggtac cgcacaatcc
     2401 agccagccgg atatgtgcca gtactgacgt ttcaggattg ccattacata cgccagggat
     2461 tcttacctgg cattcccgac cacgcgctgc ttttctcaaa tcagccatga ttcctccttg
     2521 ctgccagtcg caaccatttt ttatcaacca ggctagcggt atatccgagc agtgttggta
     2581 tttcggatgg cttcagctca ggcttacgct tacgacgatt tgatactctg tagatgtgcc
     2641 cgttcatgac acgaataagc ggtgtagcca ttacgcctcc tgcttatcgc gcagctgctg
     2701 gtattcacag ctctgcggaa ttgtcaggtg gcagccgata ttcatcgccc aggcttcgac
     2761 tttgcacagg aagatgtaca tctcgccggt ttccagctct gacgtatggc ggagggattg
     2821 gacggtggtg acctcgccgg acacgacatc tacacggtct ttgctttcgt agccgagata
     2881 ggtgtgcttc atcgcgtctt tgacccactc aggcgtagcg aaggtcttgc cgcgggcgat
     2941 gagatattcg ctgatctccg tgtaccacat gtggctgagc gcgttctgcg acaggctgcg
     3001 cttctcgcgc cacggcttaa cctgcaggcg taagcattgc ccggcatcca gcaatggctg
     3061 aatctgctgg ccgatggccg cgaagttacc gcgatggagt ttgatgccgt ctactggcag
     3121 agtcatacgg cctccctaac ggaaaccgca gaatgcagaa aatcgcaggt gcatttctgc
     3181 atctgtgaca aggtgatgag ttcagattgt ggtcgcattt aagtcccctt aaatgcgcag
     3241 aagtcaccgg agttgttcag gctccgatga gaggattatg gcggattgat tacgataaat
     3301 caaaactatc ttgaagccaa aacagtgatc ttcgctttaa aaaccttgta tactttcacc
     3361 taatcacagc cagaactatg gatctaacca atgaccaaca aagaaaacaa agataaatat
     3421 attaacaagt tcaagaaaga gctttcaaaa tatatacaac caactgctgg agtggacatt
     3481 caattattta actctaagga tggtggtggt gtaattaaag ccactttgaa tagaagtgga
     3541 aaaagaaaat catctattgc tggtaatttt actaaattag gtgaggcaat tacctcgagt
     3601 gggcagaggg tttttggtgg tgatctatca aatgtgaatt tttacggaac aaatacgatt
     3661 tttgatggag atacaatatt tttaattaaa tcacctgatt cagaggagtg gtcatcacaa
     3721 aaagcttcca aagacgtaga gggcatcgta tttggaggga aaaaatgatt aacattacta
     3781 ctaaggatgc actcaagtca gtggtgaagt aagagatcta gtaattcttc ttgaaactga
     3841 tggaaaaacg gtgtctgatt atttgcagcc aatccctccc aaaaaagata cacactggag
     3901 catgattttc attccggtag caatttatat actacttaat cttgtataca ttattgctca
     3961 cccattattt cctgaaaaat atctaaaaac atcaacatct atatttctat tcatttcctt
     4021 catattaagt gcaatagtag catattttgt tttccataag cataaatctg ccactctatc
     4081 catatgcagc ttagtgttct tagtgttaat tgtaggtgct aacgtagggt ttatcagtta
     4141 cagcgaccta acacaaaaag cgacgcaaaa attagatagt ttgtctaaat aattatgttg
     4201 ccatgcgaag tatgcatggc tgcatgtctg ccttccattt aaaatgggct aagacctata
     4261 accctaaata ttattcttta ttatcttctt tacc
//
LOCUS       DAJTYZ010000224         4289 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000224 DAJTYZ010000000
VERSION     DAJTYZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.226"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            224..1066
                     /locus_tag="OO596_004006"
     CDS             224..1066
                     /locus_tag="OO596_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6719711.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            1069..2157
                     /gene="capU"
                     /locus_tag="OO596_004007"
     CDS             1069..2157
                     /gene="capU"
                     /locus_tag="OO596_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6719712.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2162..3112
                     /gene="virK"
                     /locus_tag="OO596_004008"
     CDS             2162..3112
                     /gene="virK"
                     /locus_tag="OO596_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6719713.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            3177..4121
                     /gene="lpxM"
                     /locus_tag="OO596_004009"
                     /gene_synonym="msbB"
     CDS             3177..4121
                     /gene="lpxM"
                     /locus_tag="OO596_004009"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6719714.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         1225 a          799 c          980 g         1285 t
ORIGIN      
        1 tcaggtagcc aaattcagtg tgccacctca aggttcttag tgagtcgttt ttaaaagata
       61 tgataatata tcattgttta tgaattgttg atgtttaatt tagtaaatta tatcaaatat
      121 gccatgggtt tgctatgtta ttcatccagt atctggaaat aaaatgcacc agctttattg
      181 ggttttcctt ttgtctctgc ttactaggtg ccagaaactt taaatgttaa acgaaggggg
      241 cattttgttc aaagccaatc atgttccggt attaatgtat catcatgtca gtcattgccc
      301 cggactggtt acactttctc ccgtgacatt ccggaagcaa atgaaatggt tggctgaaaa
      361 caactggaaa acactgtcat cagatgaact ggagtttttt tatcggggcg gaaaactccc
      421 ccggaaaagc gttatgttga catttgatga tggatacctt gacaactggt ttcaggtata
      481 tcctttatta aaagagttta atctcaaagc acatattttt ttaattaccg gttttattgg
      541 gaatggtccg gttcgtcact ctcctggaaa ggaatattct catcgcgatt gcgaacatca
      601 aatagcgaca gggaatgctg ataatgttat gcttcgctgg tctgaagtaa acgaaatgtt
      661 gcaatctggt ctggtcgaat ttcatgttca tacgcatact catacacgct gggataaaaa
      721 gttcagttca cgagaagaac agtgtaaaca tcttcggcag gacttgttga gcgggagaga
      781 atatttgaaa gaaatgacag gaaaatgcag taaacatttg tgttggccgg aaggttatta
      841 taacaaggat tatattcagg tcgctgaaga acttggattc tattatttat atacaacaga
      901 aagaagaatg aatgctcctg ctaaaggaac tacacggata ggaagaataa gcaccaaaga
      961 acgggaaagt tgcgcatggc tgaaaagacg acttttttat tatacaacac cgtttttttc
     1021 atcgttgctg gcctttcata aggggccaag attaccggat gattaataat gaatatacta
     1081 tttacggaat catcaccgaa tataggtggt caggaattac aggctgttgc tcaaatgaag
     1141 gccctgaaga aaatggggca ttcagttctg cttgtctgca gggaaaacag caaaattgct
     1201 tttgaagcca gtaaattggg aattgatatc acattcgcgt tatttcgaaa cagtcttcac
     1261 atccctactg catggagatt actcggaata gttcatggtt ttcagcccaa tgcaatcgtt
     1321 tgtcacagtg ggcatgatag caatattgtt ggtttagtac ggttattcac ttggaaacat
     1381 ccattcagaa ttatcaggca aaagacatat ttgacacgaa aaacaaaagt tttttcaata
     1441 aatcattttt gcgatgaggt gattgttccc ggaacaagta tgaagacaca tctggagcag
     1501 gaaggatgtc gaacccgggt tactgttgtg cctccaggct ttgatttcca gaaattatac
     1561 gttgattccc gaaacagttt gcctccaaat gttctttctt ggctggcgtc ccgaagggga
     1621 tgccccgtta ttgctcaggt aggaatgttg cgcccggaaa aagggcacga atttatgttg
     1681 aatttactgt tccatttaaa aatgaatgga cgacagttct gttggttgat tgtggggtct
     1741 ggttcgcctg aactgcggga gcatttacag tatcagattg acagtatggg catgcatgat
     1801 gatgttttta ttgctgacaa tgtttttcct gccgcccccg tatatcgggt tgccagtctg
     1861 gtggttctgc cttcagaaaa cgaatcgttt ggtatggtac tggcagaagc atcggcattt
     1921 tctgtgcctg tactggccag tcagattggt ggaatccctg atgttattca gaacaaccag
     1981 accgggacat tgttaccagc aggtaataag cacgcatgga tgtgcgccct gaatgatttt
     2041 tttaatgacc ctgggcgttt ttatcagatg gctcgccagg caaaacagga tatagaagag
     2101 cggtttgata ttaataaaac tgcgttaaaa atactcacat tagcgaagca caagtaacaa
     2161 tatgttttct gtaagtaact tatcatttat cggtttcctt aaaaggattg ttttttcctc
     2221 agattcactc ccggggaagt gggaacacag aaaattccgg ttcatgtaca ttttgcgatg
     2281 tgctataaat ccggttgcca gtattcgata ttattacgaa ctgcgttcct tgcagtgcat
     2341 tgaggatatt ctggcaatac aacccacgtt gccagcaaga attcatcgac cttatttgca
     2401 taaagggggg cgagcctggt cccgggggca atatattctt gagcattatc gctttgtgca
     2461 aaatttgcca gaaaaatatt ctgaattttt gtttccacaa aaatcggtat cattagtcca
     2521 attcatcggg aaagatggag aggattttga tatccagtgt tctcccagtg gttttgaccg
     2581 ggaaggagag ttgatgttgt cattgttttt caataaaata gtgattgccc ggttaacatt
     2641 ttcagtcatt ctgactcaaa atggacacac cgcttttatt ggcggattac agggagcccc
     2701 caaaaatacc ggacctgatg ttattcggtg cgcaacccgg gcctgttatg ggcttttccc
     2761 aaaacggatt atttttgaag cattttgtgc gttgatgaaa gcttgtaatg tctctgagtg
     2821 tctggcagtc agtgagcaca gtcatgtttt ccggcaattg agatactggt atcagaaacg
     2881 caaaaccttt gttgctgtct acagtgattt ctgggagtct gtagcaggga aaacctgtgg
     2941 tgactggtac aaattaccaa cacaggtggt tcgaaagccg ctaagcaata tagctagtaa
     3001 aaaacgctct gagtatcgaa aacgatacgc tttgttggat tatatccatg aaactgctat
     3061 ccgctctttg gatgcatatc ctgtaaactc agaacatcag gacttaaatt aaggtttgat
     3121 gtattctctg aataaaatat taatgatgat tttggtaggg gcattcgcac taaataatga
     3181 aaaaatacaa atccgagttt attcctgaat ttaagaaaaa ttatctttcc cctgtttact
     3241 ggtttacatg gttcgttttg ggaatgattg caggtatttc aatgtttccc ccttcattca
     3301 gagatcctgt cttggccaaa atagggcgtt gggtgggcag attgagcaga aaagctcgtc
     3361 gcagggcgac gattaattta tcgctttgtt tcccggaaaa gagtgataca gaacgggaaa
     3421 taattgtcga caatatgttt gccacagcat tgcaatccat agtgatgatg gcagaactgg
     3481 caattcgtgg tccggaaaag ttccagaaac gagtgttctg gaaaggcctc gaaattcttg
     3541 aggaaatccg gcacaataac agaaatgtga tttttctggt tccccatggc tggagcgtgg
     3601 atattcctgc aatgttgctg gcagctcagg gggaaaaaat ggccgccatg tttcatcagc
     3661 aacgaaatcc agtgattgat tatgtctgga attctgtacg gcgtaaattc ggggggcgct
     3721 tacattcccg ggaggatggg ataaaaccat ttattcagtc agtacgccag ggatactggg
     3781 ggtattacct tccagatcag gatcatggtc ctgaatacag tgaatttgct gatttttttg
     3841 cgacctataa agcgacatta ccaattattg gacgtctgat gaacatcagt caggctatga
     3901 ttataccgct tttcccggtt tatgatgaaa aaaaacattt cctgactatt gaagttcggc
     3961 caccaatgga tgcatgcatt gccagcgcgg acaataaaat gattgcccga caaatgaaca
     4021 aaacagtgga aattttggtg gggtcacatc cggaacagta tatctgggtt ttaaaattgt
     4081 taaaaacgcg caaatcaaac gaagcggacc cgtacccttg aaattataaa gtacaggtat
     4141 ttccactagt tgtttcttac aggttaccaa tcgaaacaca tcccccttcc gtgtgttgcc
     4201 cgtttggcac ggaggactgt gtagatgcag ttcgttaaat ttaatttatg cctaaatagc
     4261 tgcgcttaat accgctacac ttttgccag
//
LOCUS       DAJTYZ010000225         4249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000225 DAJTYZ010000000
VERSION     DAJTYZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.227"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            245..367
                     /locus_tag="OO596_004010"
                     /pseudo
     CDS             245..367
                     /locus_tag="OO596_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(629..1195)
                     /gene="ipgB2"
                     /locus_tag="OO596_004011"
     CDS             complement(629..1195)
                     /gene="ipgB2"
                     /locus_tag="OO596_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6719715.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSREIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1478..1792)
                     /locus_tag="OO596_004012"
     CDS             complement(1478..1792)
                     /locus_tag="OO596_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719716.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(1870..1980)
                     /locus_tag="OO596_004013"
                     /pseudo
     CDS             complement(1870..1980)
                     /locus_tag="OO596_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2304..2981)
                     /gene="ospD1"
                     /locus_tag="OO596_004014"
     CDS             complement(2304..2981)
                     /gene="ospD1"
                     /locus_tag="OO596_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6719717.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKSNIINVTNSAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(3352..3489)
                     /locus_tag="OO596_004015"
                     /pseudo
     CDS             complement(3352..3489)
                     /locus_tag="OO596_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3568..3774)
                     /locus_tag="OO596_004016"
     CDS             complement(3568..3774)
                     /locus_tag="OO596_004016"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6719718.1"
                     /translation="MDAENETVLNANMTHHLGCEKNQLRSGSNSRNGCLTKIITTGDE
                     PLEIRTLRDRNGTFEPQQLKKNQP"
BASE COUNT         1158 a          868 c          815 g         1408 t
ORIGIN      
        1 aaattcaggc gaatgctgtt tacgtgtttt tttgctggtt gatactgttt ttgtcatgtg
       61 agtcacctct gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat
      121 caatttgctc actggtattt actacgtgca tcgaatgagt gatcaagagc cgtatgacgc
      181 gagagtgtca cgtacggttc cgtgagcagc tggagagtga aactctctcc ggctgactcg
      241 actcgcaggt tccgacagcg gtgacgaaca tgcagcggtg ttgttctcgc tgatcggcac
      301 acgccgtctg aacaatgtgg agccagaaaa atggctgcgt tacgttattg aacatatcca
      361 ggactgaccg gcaaaccggg tacgcgatct gttgccttgg aaagttgatc tgacttctca
      421 gtaaatatca atatgattct gacgagccgc ttacctttga gcagagaaag gggagaggga
      481 tcccacatcc atgagctagg aaaatacaaa aactggctat acaacaattc aatatattag
      541 gacttacttg cgaggagaaa tatagcaccg ctcaggcaat tcctgggtat ttacttttct
      601 tagtttcttt actcgggatg ttctgtcatc agaaaggcga ttctaaattt gtaatatagt
      661 cactcatatt cccacgtaat ttctttagat caatatattg cacatttttc tcaaaaaact
      721 gatcggaaat aagatttgca acattgcttg ttgtatcatt tttaacgctt ctattcatcc
      781 cttcacaaag ttcgtccatt ttctttttga aataactgct tcctgataca agatgattta
      841 tggaactttg tgcagcattg atgtctagag aaatgcgata atctttctca acctgtttaa
      901 atatctgttt acggaggtta tcgccgattt ctcggctgtg ttctgcacaa aacttatcaa
      961 tacattggtt taccacccgg gatacgtaga cttttttttc atcatttttc caatctgtaa
     1021 tattttcacc gatttgttct gaaacagagc tcaaaatact agtatttatt ttggttgatg
     1081 atatttttac tatccttttc cccattgtac agcggataat ctcatccacc tttcctgaaa
     1141 aatatctttt gtgagataaa ctgattccaa aattattaaa agatgttcca agcattataa
     1201 tcactttgtc atgtaaaaat agtaacattg tcattatttt agctgattca aacaattctg
     1261 taaatgcttt gagttcttta ctcccttggc tcaatgatgg accgtctgga atcccagaga
     1321 ctaaactctc tgagaaagag agtatgaaaa aacttggggc agtccctaaa tgaagggacc
     1381 aacagcggta aaataaccgg catcattcat aatgatgccc tgttagttgt agttatgaag
     1441 atttacgtca ttaaaattgc tgttcgtagt gtcagtccta tggtctggca tcagctgagg
     1501 atcgtagccg atacatcact ggcttcacga cattcgtgtt gaggctattc atgaaaactc
     1561 cacgctgaaa acgccgtttt gtataaacgg tcacggtatg cccggagcca ctgttgctga
     1621 tgaattcgat aaaaccttgg cgtttcttga agctatcgtt aatgcggata atgaaacaac
     1681 gattggtgag attcgctctt ttgccgacgc cctggacgct gtccggttca atcgcaacaa
     1741 aatcaaccgg cagctgagca aacccaacct tgcctctctg gcgctggagc atgaagtgat
     1801 ttggctggga cgtagccgct gacagcttct ataaaagtat gatcctaccc acgtaatatg
     1861 aacacaggct taagtgaggt tctggttttc aaattgttca ggactgagac ctccacacca
     1921 actgtgccgt cgtcagcgat tgtaatcgca cttgatataa ttaaacactg tatatttcat
     1981 ttgtggagtc tccagtctga gggggcttta tgcattccgt attgggcact atgatgccag
     2041 aaatctgcga ggctccacac cttcctgtat ttcccttcct ctatcacagt gcactttact
     2101 taatagggtg ggatgtgttc attacattta ttgtgttttt tatgattgtt tttgcgtcca
     2161 gtgtcgttcg tcatgggaaa ctggttctat gggtagtatc gctcagtcta gttaactgtt
     2221 tgtttctttt tagaaattaa gcagatcaca caattcgggg agagatcctt ctaagtgcaa
     2281 ttctttgcag ctatttagca cacttaaaac aactcttgta ttttgtcaga aataatatta
     2341 aacatgtctt gttcattatt gttaagtctg tctgctattt ctgtaagttt tatcccacta
     2401 tgatggcata tatttatata tgccatcata gcctcgatat tccctttccc tgcggccaga
     2461 aataatcctg gtaaaccctc attatctctt acatccaata gtttaatcgt ttcttttggt
     2521 gataaatcaa aagttttaat aaaatctcca taagcttgaa tcgcagcgga atgaccttct
     2581 tgaagagcta tgaacaaagc agattcccca gcattatttc gtgctgcaag aacttctagt
     2641 ttttcctctt tgggtatttc ggctgccaac agcgattcac cgtatttacg cgctgcatcg
     2701 gcatttccat gccaaatcaa tgaaaacaaa ttatggcggt tatactcctt attattcttt
     2761 gtcggaatgg atatgctctg tattttggcc aattttctga aagaaacacc actatatccc
     2821 ccccctgatt tttcttcatt gatggcgtgg gatatagcgg aatttgtcac gttaatgata
     2881 ttacttttca ttcctttatt ttcattagca gtattgctgc ctattattag gtgcatattt
     2941 ttgttgtttg ctggatgtaa tccatagtta tttattgaca tttttgttca tcctttttgc
     3001 taaaaaaaac aataacctat ttttgtaatg taatagatat tagtctattg ccaatctaaa
     3061 aaaaccattg ccacattgaa tggctatatt caccaataaa ttctttcgat ttcgtatatt
     3121 gacatatcgt agatatatct tatccctaag attcaataat cgagtccgtt taccattctg
     3181 tgcaagcgcc ttcactcaga agtgaccgtc aaggcgatca ccaaacttga taataaagca
     3241 gctcattgcc atacgccagt ccttaagggg cgttgtccat ttctgtgatg ctgcctagat
     3301 ttaactgcca gctccggctt atctacatct aaatatgtga gcctaatacc gctacacttt
     3361 ggccagacta tgtttttctc cgggaagtgg gctaaccgtc tccataaagt gacgaacttt
     3421 tttcaacagt ggccatattg aacggcactg atgattacgg gttattgtct catgaagagc
     3481 cggccacatc actgacagca gtgacttcag gccttcagca tcagacagcc tgaaccagtt
     3541 aatccatcgc ccgacagatt tagtaatcta gggctggttt ttcttcagct gttgcggttc
     3601 gaaagtgcca ttacgatcac gcagagtacg gatttccaga ggttcatctc cagtagtgat
     3661 aatcttcgtt aagcaaccat tgcgggaatt gctgccagat ctaagctgat ttttctcgca
     3721 cccaagatga tgagtcatat tagcattgag tacggtttca ttttcagcat ccaatcaaac
     3781 tgactgagat cttcaggatt tttgagactt ttggctagtt cattagctaa agcccgcaac
     3841 ttattagatg tcaactgttt ttcattcata aattcacctt catttgatat caaattaaat
     3901 agttcaacat cagagaatta tacaaactta tgtacaggct cctatgatct tcgaatcctg
     3961 aagattatag tgaaaataag ttttcacgtt gaaatcccac atagttagta tatgataaat
     4021 aatcatggat gacctgatta acctcttggg gtagtgcttt tatagaagaa taaatattct
     4081 actaatttaa tacatcacct aaaatagtat tgttttttat ggttggttga aggtcgtggt
     4141 gatgatgtta gtccttttga gaatattcca gtgccagatc ttacttataa taaaggggct
     4201 tggcagtttg gataaactat tgttcttatt ccactaatac aaacttttt
//
LOCUS       DAJTYZ010000226         4237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000226 DAJTYZ010000000
VERSION     DAJTYZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.228"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="OO596_004017"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="OO596_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6719719.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OO596_004018"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OO596_004018"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR6719720.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="OO596_004019"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="OO596_004019"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCR6719721.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4086..>4237
                     /locus_tag="OO596_004020"
     CDS             4086..>4237
                     /locus_tag="OO596_004020"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6719722.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIA"
BASE COUNT         1046 a         1078 c          976 g         1137 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgtga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgctcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc
     3841 atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac
     3901 tctatgtgaa ataaatcaaa atttcacgct gcaatactcc ttagaatgta tagcgaaagg
     3961 agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa ggttatcaac
     4021 ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat
     4081 ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg
     4141 cggaaatcgc ccaattcctg catgaaaatg atttgagcgt tgacaccaca gtcgaagtat
     4201 ttattaccgt aacccgcgat gaaaagctta tcgcgtg
//
LOCUS       DAJTYZ010000227         4236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000227 DAJTYZ010000000
VERSION     DAJTYZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.229"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..145
                     /locus_tag="OO596_004021"
     CDS             <1..145
                     /locus_tag="OO596_004021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719723.1"
                     /translation="RLRALLQTQQDTIHQMAEYNRLLSQRMAAYASEINRLKALVAKL
                     QRM"
     gene            complement(214..276)
                     /locus_tag="OO596_004022"
                     /pseudo
     CDS             complement(214..276)
                     /locus_tag="OO596_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            727..2139
                     /gene="ospC1"
                     /locus_tag="OO596_004023"
     CDS             727..2139
                     /gene="ospC1"
                     /locus_tag="OO596_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6719724.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSIANQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2243..2392)
                     /locus_tag="OO596_004024"
                     /pseudo
     CDS             complement(2243..2392)
                     /locus_tag="OO596_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2468..4165
                     /gene="ospD3"
                     /locus_tag="OO596_004025"
     CDS             2468..4165
                     /gene="ospD3"
                     /locus_tag="OO596_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6719725.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1501 a          735 c          764 g         1236 t
ORIGIN      
        1 ccgcctgcgg gcactgttgc agacgcaaca ggacactatc caccagatgg ctgaatacaa
       61 ccgcctgctc tcacagcgga tggcggctta tgcttccgaa atcaaccgac tgaaggcgct
      121 ggttgcgaaa ctgcagcgta tgtagttcgg taaaagctca gaaaaacttc gcgcaaaaac
      181 cgaacggcag attccatttt cacgggcgat ctacgccaca caggctctgg gttgcagtga
      241 ctgctccatt aaggcgattt tgaactcata agggaagtaa caaaaaaact gcaaaagtac
      301 aataattata tatggccaaa ctaacctcta ttaaaaaaac atcagttttt cacacttagt
      361 tgagcttcat gaaaaaatca tcgaggattt cattgcagaa tacacattcc actaactgga
      421 agcactccat aagaggaatc agagaaagta tcgttttttt atagtaaaat tcattgctgt
      481 tcaataaaat tgatatagtt attaaattat gaaaataatt tcacaaaaaa gattaacttg
      541 agttcttaag catcggattt tagatatttt gaaatatctg gcatcacata acatcaaaag
      601 tatataacac ccttatctaa ttacatgcag tatattgtat atatactcaa aaattgtaat
      661 tcataccctg ctatatcttg tagggaaaga tattaattaa aactgttttc atataaggtt
      721 cattttatga atatatcaga aacactgaac tcagcaaata cccaatgcaa tatagattct
      781 atggataaca gattacatac attgtttcca aaagtgacat cagtgcgaaa cgctgcacaa
      841 caaactatgc cagatgaaaa aaatttaaaa gatagtgcaa atattattaa agatttcttt
      901 aggaaaacta tagcagcaca gagttatagt agaatgttct ctcaaggctc taactttaaa
      961 tctttaaata tagcaattga tgcaccatca gacgctaaag cctcatttaa ggctattgag
     1021 caccttgaca gattatcgaa gcattatata tctgaaataa gggaaaaact tcatcctctt
     1081 tctgcagagg aactcaattt gctttcgcta attattaatt ctgatttaat cttcagacat
     1141 caaagtaatt ctgatttgtc tgataaaatt ttaaacatta agtcattcaa taaaattcag
     1201 tctgaaggaa tatgcacaaa acgaaacaca tacgctgatg atataaaaaa aatagctaat
     1261 catgactttg tgttttttgg cgttgaaatc tctagccatc agaaaaaaca ccccctgaat
     1321 acaaaacatc acactgttga ttttggtgca aatgcgtata tcattgatca tgactctcca
     1381 tatggatata tgacattaac cgatcacttt gataatgcta ttccacctgt tttttaccat
     1441 gagcaccaat catttttaga taaattttca gaggttaata aagaagttag tcgatacgta
     1501 catggaagta aaggaattat agatgtacca atattcaata ctaaagatat gaagttaggg
     1561 ctcggattat acctgattga ctttattaga aaaagtgaag accaaagctt caaggagttt
     1621 tgctatggaa aaaatcttgc ccctgtggat ctggatagaa tcataaactt tgtttttcag
     1681 ccagagtacc atatacctag gatggtaagt acagaaaact tcaaaaaagt taagattaga
     1741 gaaatatcct tagaggaggc tgttacagca tctaattacg aagaaattaa caagcaggtc
     1801 actaacaaaa aaattgctct ccaggctctt tttctttcga ttgctaatca aaaagaggat
     1861 gtcgccttat atatattatc taattttgag ataactagac aagatgttat ttccataaag
     1921 catgagttgt atgatattga gtatctactt agcgctcata attcaagctg taaagtactt
     1981 gagtatttta tcaataaggg attggttgat gtaaacacaa agttcaaaaa aactaatagt
     2041 ggggattgta tgttggataa cgcaataaaa tatgagaatg cagaaatgat aaaactatta
     2101 ttgaaatatg gtgcaacatc tgacaataaa tatatttaat caaaattgaa tatcgtttag
     2161 caaaaggcag tatttgcaca aaatatcgat atattgttta ttgtcagtat ggctcaatgt
     2221 gataatagtt ggaaagtttg atgggtttcg ccccgttgta gcggtagtcg agctggaagg
     2281 tcttcaggca ctgcttacag cgatagagca gcccccctga actggaatgg ccgttccgga
     2341 tacccccctg agtttcagag taacggggac aaaccacatc aatctttgcc atcaatcatc
     2401 caaagggcaa agagtacaac aacactaagt ctgcgtcaca acccatcaat gaaaggaata
     2461 tatacatatg ccatcagtaa atttaattcc atcaaggaaa atatgtttgc aaaatatgat
     2521 aaataaagac aacgtctctg ttgagacaat ccagtctcta ttgcactcaa aacaattgcc
     2581 atatttttct gacaagagga gttttttatt aaatctaaat tgccaagtta ccgatcactc
     2641 tggaagactt attgtctgtc gacatttagc ttcctactgg atagcacagt ttaacaaaag
     2701 tagtggtcac gtggattatc atcactttgc ttttccggat gaaattaaaa attatgtttc
     2761 agtgagtgaa gaagaaaagg ctattaatgt gcctgctatt atttattttg ttgaaaacgg
     2821 ttcatgggga gatattattt tttatatttt caatgaaatg atttttcatt ccgaaaaaag
     2881 cagagcacta gaaataagta catcaaatca caatatggca ttaggcttga agattaaaga
     2941 aactaaaaat gggggggatt ttgtcattca gctttatgat cccaaccata cagcaactca
     3001 tttacgagca gagtttaaca aatttaactt agctaaaata aaaaaactga ctgtagataa
     3061 ttttcttgat gaaaaacatc agaaatgtta tggtcttata tccgacggta tgtctatatt
     3121 tgtggacaga catactccaa caagcatgtc ctccataatc agatggccta ataatttact
     3181 tcaccccaaa gttatttatc acgcgatgcg tatgggattg actgagctaa tccaaaaagt
     3241 aacaagagtc gtacaactat ctgacctttc agacaatacg ttagaattac ttttggcagc
     3301 caaaaatgac gatggtttgt caggattgct tttagcttta caaaatgggc attcagatac
     3361 aatcttagca tacggagaac tcctggaaac ttctggactt aaccttgata aaacggtaga
     3421 actactaact gcggaaggaa tgggaggacg aatatcgggt ttatcccaag cacttcaaaa
     3481 tgggcatgca gaaactatca aaacatacgg aaggcttctc aagaagagag caataaatat
     3541 cgaatacaat aagctgaaaa atttgctgac cgcttattat tatgatgaag tacacagaca
     3601 gatacccgga ctaatgtttg ctcttcaaaa tggacatgca gatgctatac gcgcatacgg
     3661 tgagctcatt cttagccccc ctctcctcaa ctcagaggat attgtaaatt tgctggcctc
     3721 aaggagatat gacaatgttc ccggacttct gttagcattg aataatggac aggctgatgc
     3781 aatcttagct tatggtgata tcttgaatga ggcaaaactt aacttggata aaaaagcaga
     3841 gctgttagaa gcgaaagatt ctaatggttt atctggattg tttgtagcct tgcataatgg
     3901 atgtgtagaa acaattattg cttatgggaa aatacttcac actgcagacc ttactccaca
     3961 tcaggcatca aaattactgg cagcagaagg cccaaatggg gtatctggat taattatagc
     4021 ttttcaaaat aggaattttg aggcaataaa aacttatatg gaaataataa aaaatgaaaa
     4081 tattacacct gaagaaatag cagaacactt ggacaaaaaa aatggaagtg attttctgga
     4141 aattatgaag aatataaaaa gctgaatatt atctcgtggt gatacactga atttgaggta
     4201 accggctcat ttaaaccgtc tggtctgtta cctccg
//
LOCUS       DAJTYZ010000228         4192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000228 DAJTYZ010000000
VERSION     DAJTYZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.230"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO596_004026"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO596_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6719726.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="OO596_004027"
     tRNA            complement(330..405)
                     /locus_tag="OO596_004027"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="OO596_004028"
     tRNA            complement(412..486)
                     /locus_tag="OO596_004028"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="OO596_004029"
     tRNA            complement(603..687)
                     /locus_tag="OO596_004029"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="OO596_004030"
     tRNA            complement(696..771)
                     /locus_tag="OO596_004030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="OO596_004031"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="OO596_004031"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6719727.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OO596_004032"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OO596_004032"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6719728.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OO596_004033"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OO596_004033"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6719729.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1042 c          936 g         1134 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAJTYZ010000229         4176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000229 DAJTYZ010000000
VERSION     DAJTYZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.231"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(108..1472)
                     /locus_tag="OO596_004034"
                     /pseudo
     CDS             complement(108..1472)
                     /locus_tag="OO596_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1537..1656)
                     /locus_tag="OO596_004035"
     CDS             complement(1537..1656)
                     /locus_tag="OO596_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6719730.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1811..2029)
                     /locus_tag="OO596_004036"
     CDS             complement(1811..2029)
                     /locus_tag="OO596_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6719731.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2383..3861)
                     /locus_tag="OO596_004037"
     CDS             complement(2383..3861)
                     /locus_tag="OO596_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HCR6719732.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1185 a          915 c          727 g         1349 t
ORIGIN      
        1 cctaagacat caatcatctg ctctccaatg actagtctaa aaactagtat taagactatc
       61 acttaaataa gtgatactgg ttgtctggag attcaggggg ccagtctagc aactaaagca
      121 ctgcccgtct cctggttaac cccatgtgct ataccgccgg tatagacaat cagcgagcct
      181 ccggcgctaa tattgctgcc agttgccgta ccatctttct caacaacctg ccggctcccg
      241 gtggaaatat ttgtcgtgtc agctttcccg ccgctcttga tattttgtgt tccgctgttg
      301 atattggtac ccgtggcaat accatgatta ttaatattct gtgtaccacc attgattatg
      361 gtattggtcg cgtttctggt aacatccatt accccgccgt tatctattcg ggtcgcatca
      421 gctttagcat tcgttaaaac tgacattgtt cctttatctt taataatcgt cttatttgcc
      481 gaaccatatg cgtttatgtc taaatgacca ccgttttcca gcaacacatt gtctgccacg
      541 tgattgtgga tggagaatgc accttcacta ttcgtaccgc tcaccgtcgt accgttagtg
      601 tttgttttta aaattgcacc atcgtgctgg gtaacatttg ttgccgtacc accactaaca
      661 tcaagcacgc caccggaata aacttcaata acatccgagg agctggtgta atcaacaatt
      721 tgcgtaccac cagaatagat ctgagtattt tttgccgttg acttattatt cagagactga
      781 gttccacctt caatcgtcgt gtccagcgca tggctttcat atactctttg ctcaccgcca
      841 tttttaatgg tggttgttcc tactgtgctc tgttcaacat actgtcgtcc accatttatg
      901 attgtgttcg ttgccatact tccttgcgtg atgtcctgat aaccggactt atttatcgtt
      961 gtaccatcaa catgcccctg agttgttact gtctgccctc caccatcaat gatggttcca
     1021 ttcgcagtcc ccccacttat gtgtgaatta ccacccttaa ttgtcgttcc attgctgata
     1081 ccgcctgtat aaacgtcctg attgccactg tcgattatcg taccggtgga aatgcccccg
     1141 tcatgaattg tctgtcttcc tccgttaatg gttgtattat tagactgccc catataattg
     1201 ttattccttc caacatcttg atatccacca gattcaataa gacttccatt agatactccg
     1261 ccataaacac tctgctggcc atggttgata atatgagtgt tatttgttgt acctcgttca
     1321 tctactcgtt ggttgccatc tacagtctca tcgtttacca caccaataac attagtagta
     1381 aaggccgcca tcacgggcgg ggcatatatc aaggcagata tcaataagga aagtactgag
     1441 cggcgacaat aatggggact ggtcctgttc ataaatttca tcctctgaaa agtgaatact
     1501 gagtagcgtt taagcgactt tagctctgct gcaacatcag cccgcaggca ccagaccggg
     1561 gggttcatcc tgaagagaca gcgcaagtgt attgtgttca ccgctcatca aagacatcat
     1621 gatgaaatga tgatgttcca cataagaaag aggcattttt taaacacagt gcgctgaagt
     1681 gtgattggat aaaaaagtca atctattcag gaaatacggg cgtattcttt tctttcgaca
     1741 atcaggccgt cggcaaaata aaatgattta cataatcgtt tctgatgaat atcttctgct
     1801 cacataaaaa tcacacaata actttgagat cgcagattct tttactttta cagcattcgt
     1861 ccccccattg ttgggcaaat atagattggg ccagagcacg aaagttaata ccacgtttgc
     1921 acagctcctc caacagcacg acaagatgcc acatactgcg ccccagtcgg tgcggtttac
     1981 agactacccg tgcgtccacc gccgataatg tcctgagcag ttttttcagt ccggacctcc
     2041 tgccacggtt ttacgttgaa gaggtaaccc tgagcacgca gttcttcagt caggcgtggt
     2101 gcaccgtaac gctgttattg ctgggtaaga tcaaaaaact ttcaggcagc taaggaaagt
     2161 tgaaccagac attagatgaa atatttcaac caattacagc accaatttaa actcagtaaa
     2221 tcacagcaca gatcaagggg ttacgtgaaa gcgtagcccc tttttctttg gtagtggcag
     2281 caaaatggtt gtagtgtaaa aaataatccc gtttaatcaa tcaataatac atattgtttc
     2341 aatctacgtt attatctctt tgtaaaaata gccatttatt aatcattgaa aactgctttt
     2401 agaacttgat acaacgggac tagtcacaac aggactattc tcaacgggat catcctcaga
     2461 ggaactatca tcaaagtcat catccataaa taaaatatca tcgaatggtg ccacgcccgt
     2521 gatgagtttt attttattat tacgatcagt caagactcca cttaaaccgt tttcgctcac
     2581 aggttttaat gatttttcat tactcttgtt gtaagcaggc gcattaaaaa tacacggagt
     2641 atcaagatca aacaatgacg ttctccagtt cacatattga atatcatagt tactgaagtt
     2701 ctgtccagaa aagaagcatc ccttaaaatc caatccacct aaattatata aaccatcctc
     2761 ttctttttgg agagtaatgt taattctggc tatctcccgg acaaaacttc cttttttgta
     2821 tttgaatacc gtttcaagat tttcaccaga cagcttgact tctgggaata atttaaaatt
     2881 aaaccctaca tcattattat tcaaagtaga tgaacgaaag atggaaaaag cttgtagtgc
     2941 tgaattatag ctttcgattt tatcttgagg ctgcgctctt ggtaaaaact tatagcagca
     3001 ctcataaaaa ttgaataaac gttctgaaca attcccattt ttatcaatta ataccccccg
     3061 gaattctttc acaaaggcat ccgttatttt ttgaatatct atggtgtgtt cctttgactc
     3121 tgacaaagat ggagtctgtt gaagtgcatt gctcatctgg ttaaggaaca tctgcatttc
     3181 tttaaattta tctccatagt ggcctttaac ttgctgagag tttttagtta caggtgaaac
     3241 cttatctatt tggttgtcat ataaattatg tggttccata cagacatttg gttgtgtttc
     3301 taaaataaca ctacgtgtac tacctggctt aaatgaatca acatgaaaat cactttttct
     3361 tatattatcg aatagggtct gttcatttct cttagcgcat tcaataaact gatcaacatt
     3421 atttttattc gatgacaggt cagtttcaga aaccgcattt tctttgttct ccagctttag
     3481 tttaatgaga agatcttccc agacctgctt gcttaaacat actacgtcag gctcaccatc
     3541 agtaactaac tcttcattat tatcaaagta tacgttgaat acttttaagt tattttcatc
     3601 aacatcatat ttaatacgtt ttaattgctc tccagatccc ataatgacaa aggcgttgcc
     3661 ttcatgatat atacattcag acatcatttt ttgtaaagtt tcaagagcac cgcgatacgt
     3721 ccctgaggca gcttccttac aaattaaatt tataatacta taagctagta gctccatgga
     3781 ttcagaagat tttctttcat tacaatttcc actatctata aatgagctag tgttaaattc
     3841 gttgctttta ctaactaaca ttgtctattc ctcagttaat gtctacatgg ctatttttaa
     3901 ttttattatt gtttgtcact ataaaaaatc gctcatttga gacaattgct ggcattaaca
     3961 gcttcatatg ctatacatgg tacttttaat taaattagca caagaatgtt aaatttaata
     4021 aacaaaaagt tatttcgctg tatgataaaa accacccgtt ataatttatt agtgaaattc
     4081 gtttttcgag tgttagaaat ttatatctaa atagcgttga ttaatgagca aagccagact
     4141 cctgtaacgc tcacaagact catctacctg cgacgg
//
LOCUS       DAJTYZ010000230         4132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000230 DAJTYZ010000000
VERSION     DAJTYZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.232"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            649..1526
                     /locus_tag="OO596_004038"
                     /pseudo
     CDS             649..1526
                     /locus_tag="OO596_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1526..1816
                     /locus_tag="OO596_004039"
     CDS             1526..1816
                     /locus_tag="OO596_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719733.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            2137..2427
                     /locus_tag="OO596_004040"
     CDS             2137..2427
                     /locus_tag="OO596_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719734.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2427..2846
                     /locus_tag="OO596_004041"
     CDS             2427..2846
                     /locus_tag="OO596_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719735.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            3014..3337
                     /locus_tag="OO596_004042"
     CDS             3014..3337
                     /locus_tag="OO596_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719736.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            3767..>4132
                     /locus_tag="OO596_004043"
     CDS             3767..>4132
                     /locus_tag="OO596_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719737.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRL"
BASE COUNT         1366 a          751 c          837 g         1178 t
ORIGIN      
        1 cataacaggc ttactattag gtcaccgttt ccaagtgcag tcgttgtttt taaaaaagaa
       61 ccacgtaagc atgaaattac acagatggtt tgctgtgctg atacattgct accgttaacc
      121 atcattaacc gtggtggact aagaggaaga cccaccattt acccacctga gacgctcgac
      181 agcttcataa aactatacag gcaaggaaaa cccattaagt acattgcaga tgctctcaga
      241 atgcctctga gcacgtcata caggattgct caaaggttga gctaaaattc tcataaatat
      301 ggtcatttac aagacaggtg attcagatgt taagaagtat ccctgtgaac aataaatgac
      361 caatcagtaa tcattctgtg aaataattgt tgcaatttat aatcgcatct gttatagttt
      421 ctatatgggc tgataaaaaa gttcatgtct tgaagtggta acacttgggg aggtgtcaga
      481 tggttggcgt atattaacct ctcattcagg gtatacctca gcgttttaat catcgtactg
      541 tttcaggcat ggacttcttt atcagtccat caatccccca gatattttaa tcacacaggc
      601 taatcggttt tatccggttg gccttttcac gtatgaggta atttatgaat gtctaaatat
      661 aacccaaatc agttttataa atttgatgtg cgaatctctc aactttcaga agaaattaac
      721 gcagcagcaa gattgattta tatggtcatg gtccatagct gtgacttcta tgaagagaaa
      781 tacaaatcat attcaccaag tattgaccat ctcgcttata tggctggtgt atcaaagaga
      841 acagcaatca gagccattga gacacttgag aaacacggtt taattcaggt gcaaaagaat
      901 caggggcgtc aaagcactta tcgtgtattc acactggatg ataagccgga attacttgta
      961 agacctacat cacaggaagt tgcagcctca gtaagacaaa atgacaccac taccagtgac
     1021 aaaatgtcat gggggtagtg acaaaatgtc acccgtgtta gagtttagat aagttattag
     1081 ataataatat tacagataag aataaagaga ctggacttaa tgatgatatt aaagaagatg
     1141 actttctttc tttggatgaa ttaaatgatt ttctctctat gaatgaatct gaccttgaag
     1201 taagctctaa aaagacttct aaacaagaca gagaaatcac tgatgtgagt gttgatagtg
     1261 accataaaaa cacacagtgc cccgccactg atgatgagtt ggaccccttc gatgatgaag
     1321 aggatgaacg tgaaaaagcc attaagaggg cagagaagaa aaagagattc gagcttgcaa
     1381 gacaaaagaa caaagtatcg ggtttagacc cattcgatga cagtgaagat gattattatc
     1441 tttcaccgaa aaagaaacaa actcaggatg acaagaagca aaaaattaaa ccgaagatgt
     1501 attttgccgg tggaggaatt aagtaatgaa agaatatcac ggtgaaaaga gatacaaaga
     1561 ttacctgtta aagcgttaca gcatttctcg tgaaggtcat cttatgaagg atactcacgg
     1621 ggaagtttac cggattagac caaagaaaga agggaggggt tatttctttt acgatggtgt
     1681 gacagattta aagattgacg ctttgaggtt tgcagtgctg tatcattttg atatctggaa
     1741 ttcaatccac cagctacgat taaaagacgg tgaccccagc aacttaaagg ccaccaatat
     1801 tatcacaaaa cgttagtttg ttttaccatc atcaatcgta attgtgattt catatttgaa
     1861 aagcttaatg ctgaaattga atcgtgtact tctggcgata atcattttta aaagctgata
     1921 cacaagaagc accagaaaca caaagcaacc taagcttgcg aatctgtgag attcagcaat
     1981 tgtttcaaga aagcccatta attcccctta tttatcaaag ggaatgacct gtatcagccc
     2041 cctacaacca tattaacatg taaatgagtt gttgacaata tgttataaat atgttaaagt
     2101 tagttataag tcaaagattt taagaaggaa aatttaatga gacacacagt tacacgcctt
     2161 ttgccagagg catattccag actggcagca atcaaagaca aatacccagc catgagccac
     2221 acagcacttg ttggtgagat tttgaaggaa cacatagccc ttacaccgtt tgacgctatc
     2281 aagggcgttg cagagggttc taaggccatc agaatcacaa cgggccaact ggaactactc
     2341 aaagcacagt ccgcagtggc aggttgctca atgattcagg tgttgagtgc caaggttcat
     2401 caactggcgg ataagctggg ggtttaatgc tatgcacatt tgattatcat gcgttttatc
     2461 gccatagagg tcaccttgtc cgtgagaata acggaacatg gaaagattta actcgtaatg
     2521 ctgaaccctt cacacaccag caattaaaat cccgcttctc cagatggaag aatgcaccag
     2581 cagatactgg tgattttgag attatccata ccttcgatta tgcctcagaa gatgaccttc
     2641 acgatgcaat taagctgatt gaagacttca atacctcaga agatgtggat ggtgacaaat
     2701 ataaatcccg tggtaaccgc tataaccgtg atgatatcac tcaggccata aatgctggtc
     2761 aatctcagcg atacattaca gaaaaatatg gtatttcacg gagacagtat ttctacatta
     2821 agccattgat taataaagac aattaaaaat aaccatctaa gaaacggtag aatgcacttt
     2881 acaggcagag ttgcacctta atttgataaa ttaccttacc agccagtgca actccctctg
     2941 taacccgcat ggttactggc ctcagaatgg ctattttaag aggtctgagg tgtgtttcat
     3001 aggagattta aaaatgaata attacccaat gcaaattatg gttgataacg acactgctat
     3061 gatggtgcaa agcttcattg atgcaggtat cgcaattaat tttgataaat tactaaagct
     3121 gatggctgat aatgtagaaa acatctctga ctttattcag tcggtggagt ttaacgagcc
     3181 tcgaatgatg ctaccaatta cagacagcaa catgaaaaga ttggttatcg agcaaacgaa
     3241 catatacagt atttccccag agcaattttt gaaaggtgct gtgacaatcc tgtacagtga
     3301 taatattctg gtgacggatt ctgtgagggt tcactaatgg gtttaataat tgcttggacg
     3361 ttgcttggct tatttgttgg ttgtattatt aacaatgcgg taaataagaa ttgatgttat
     3421 caacctgtta aaaagctgtt gacaaataac cttaagcatg gcataatttt aattatggga
     3481 gagcaattct ctcttatttt tctttcaata atccgatgga tggatattct gagttggaaa
     3541 ggtgcaatgc ttttcgtcat ggcctttcag gttagtctga gaggctatct caaaaagtac
     3601 tcttgttcat tcattattga atcctttgcc cttcacagaa atgtttaggg ctttttcagt
     3661 ttttaaaagc acacttggca ggttcttgtt cagtcagact cttcacctct gattgctgag
     3721 tgtgcttcta taaattgaaa taacaaataa actaaggggt gaacgaatga ttagaaatga
     3781 acatgaaatg cttatcgaac acagaccaag tgcacttgat attcacgaat taactttgcg
     3841 tgaatctttt gatagagctt ggcgggaatc acacgaagta aataaaggtc catatattcc
     3901 accagaacca ctcgagattc caagagttga aattgatttt tctatgaacg aacggtgtga
     3961 acttgatttg aagttgaagc tacaacgccg atatttccaa gctattaaag actctcaaga
     4021 agcaatgaga agtgtttacg agaaaattca gtccaagctt gaagacgaaa ttgacgaaat
     4081 ggttatttcg atggagcttc aaaccaattc ttatgcttat ttctctcgac ta
//
LOCUS       DAJTYZ010000231         4087 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000231 DAJTYZ010000000
VERSION     DAJTYZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.233"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..226
                     /locus_tag="OO596_004044"
     CDS             <1..226
                     /locus_tag="OO596_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6719738.1"
                     /translation="AKGKATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAA
                     IGFLLALFIGLRSRKKTRHHSLKENVAPGG"
     gene            453..1718
                     /locus_tag="OO596_004045"
     CDS             453..1718
                     /locus_tag="OO596_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6719739.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1730..2596
                     /gene="ydiB"
                     /locus_tag="OO596_004046"
     CDS             1730..2596
                     /gene="ydiB"
                     /locus_tag="OO596_004046"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6719740.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2627..3385
                     /gene="aroD"
                     /locus_tag="OO596_004047"
     CDS             2627..3385
                     /gene="aroD"
                     /locus_tag="OO596_004047"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6719741.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3529..>4087
                     /locus_tag="OO596_004048"
     CDS             3529..>4087
                     /locus_tag="OO596_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719742.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFI"
BASE COUNT         1075 a          923 c          980 g         1109 t
ORIGIN      
        1 cgctaaaggt aaagctacag ggatctatta cagtgcgggc agtattgcga cctttactat
       61 tccgttgatt acggctcatc tgtcccaaag aagtattgcc gatattatgt ggttcgatac
      121 cgccatcgct gccatcggtt ttttactggc actgtttatc ggcttacgca gccgcaaaaa
      181 aacgcggcat cactcgctaa aggaaaatgt cgctccgggt gggtaatgca atattctttt
      241 caggtcatgc aagatcttac ggataaataa ctctttctgc gctaactaag gaaaatcgcg
      301 atcaaaaaca aactatgaca tgcaatattc ctggaaacat aaactttatg ccatgtaccc
      361 agggaaaatc atcttcagta tagtaattat gtaaaccgtc ggagaacaat acgtacggta
      421 acgaaattat ctttcagcaa ggagctgtga aaatgtctca aaataaggct ttcagcacgc
      481 catttatcct ggctgttctt tgtatttact tcagctattt cctgcacggc attagtgtta
      541 ttacgcttgc ccaaaatatg acatctctgg cagaaaagtt ttccactgac aacgcgggca
      601 ttgcctattt aatttccggt atcggtttgg ggcgattgat cagtatttta ttcttcggtg
      661 tgatctccga taagtttggt cgtcgggcgg tgatattaat ggcagtaata atgtatctgc
      721 tattcttctt tggtattccc gcttgcccga atttaactct cgcctacggt ctggcagtgt
      781 gcgtaggtat cgctaactca gcgctggata cgggtggcta ccccgcgctc atggaatgct
      841 ttccgaaagc ctctggttcg gcggtcatac tggttaaagc gatggtgtca tttgggcaaa
      901 tgttctaccc aatgctggtg agctatatgc tgctcaataa tatctggtac ggctatgggc
      961 tgattattcc gggtattctg tttgtactga tcacgctgat gctgttgaaa agcaaattcc
     1021 ccagccagtt ggtggacgcc agcgtagcta atgaattacc gcaaatgaac agcaaaccgt
     1081 tagtctggct ggaaggtgtt tcatcggtac tgttcggtgt agccgcattc tcgacctttt
     1141 atgtgattgt ggtgtggatg cccaaatatg cgatggcttt tgctggtatg tcagaagctg
     1201 aggcattaaa aaccatctct tattacagta tgggctcgtt ggtctgtgtc tttatttttg
     1261 ccgcactact gaaaaaaatg gtccggccca tctgggctaa tgtatttaac tctgcgctgg
     1321 caacaataac agctgccatt atctacctgt acccttctcc actggtgtgt aatgccggcg
     1381 cctttgttat cggtttctca gcagctggcg gcattttaca gctcggtgtt tcggtcatgt
     1441 cagagttttt ccccaaaagc aaagccaaag tcaccagtat ttatatgatg atgggtggac
     1501 tggctaactt tgttattccg ctgattaccg gttatctgtc gaacatcggc ctgcaatata
     1561 tcattgttct cgattttact ttcgcgctgc tggccctgat taccgcaatt attgttttta
     1621 gccgctatta ccgcgttttt attattcctg agaatgatgt gcggtttggc gaacgcaaat
     1681 ttagcacccg gttaaacaca attaagcata gaggttaaag gagttaatta tggatgttac
     1741 cgcaaaatac gaattgattg ggttgatggc ttatcctatc cgccacagtt tatcgcctga
     1801 aatgcagaat aaagccttag aaaaagcggg attgccattt acctatatgg ccttcgaagt
     1861 ggataacgat agctttcccg gagcaattga aggattaaaa gccctcaaaa tgcgcggaac
     1921 tggtgtgtcg atgccgaaca aacaactggc gtgtgaatat gttgatgaat taacgccagc
     1981 agccaaactg gtgggtgcca tcaacaccat cgttaatgat gatggctatc tgcgtggcta
     2041 taacaccgac ggcacgggcc atattcgcgc cattaaagag agcggttttg atatcaaagg
     2101 caaaacgatg gtgctgttag gggccggagg tgcctcaaca gcgattggcg cgcagggggc
     2161 aattgaaggt ttaaaagaaa ttaaactctt taaccgtcgg gatgagttct tcgataaagc
     2221 cctcgccttc gcgcagcggg ttaacgaaaa caccgattgt gtcgtcacgg ttaccgatct
     2281 cgccgatcag caagcctttg ctgaagtcct ggcttccgcc gacattttaa ccaatggcac
     2341 aaaagtaggt atgaaccccc ttgagaatga atcattggtt aatgatatca gtctgttaca
     2401 tccgggactg ctggtcactg aatgcgtgta taacccgcat atgacgaagt tattgcagca
     2461 ggcacaacaa gcaggttgca aaacgattga tggatacggc atgttgttgt ggcaaggggc
     2521 tgaacagttc acgttatgga ctggcaaaga tttccctctg gaatatgtta aacaggtcat
     2581 ggggttcggt gcctgacagg ctgaccgcgt gcagaaaggg taaaaaatga aaaccgtaac
     2641 tgtaaaagat ctcgtcattg gtgcgggcgc acctaaaatc atcgtctcgc tgatggcgaa
     2701 agatatcgcc cgcgtgaaat ccgaagctct cgcctatcgt gaagcggact ttgatattct
     2761 ggaatggcgt gtggaccact ttgccgacct ctccaatgtg gagtctgtca tggcggcggc
     2821 aaaaattctc cgtgaaacca tgccagaaaa accgctgctg tttaccttcc gcagtgccaa
     2881 agaaggcggc gagcaggcga tttccaccga ggcttatatt gctctcaatc gtgcagccat
     2941 cgacagcggc ctggttgata tgatcgatct ggagttattt accggcgatg atcaggttaa
     3001 agaaaccgtc gcctacgccc acgcgcatga tgtgaaagtt gtcatgtcca accatgactt
     3061 ccataaaacg ccggaagccg aagaaatcat tgcccgtctg cgcaaaatgc agtccttcga
     3121 cgccgatatt cctaagattg cgctgatgcc gcaaagtacc agcgatgtgc tgacgttgct
     3181 taccgcgacc ctggagatgc aggagcagta tgccgatcgt ccaatcatca cgatgtcgat
     3241 ggcaaaaact ggcgtaattt ctcgtctggc tggtgaagta tttggctcgg cggcaacttt
     3301 tggtgcggta aaaaaagcct ctgcaccagg gcaaatctcg gtaaatgatt tacgcacggt
     3361 attaactatt ttacaccagg cataagcaat aatatttcgg cgggaacacc ctccccgccg
     3421 aacttaaaaa atatatttaa ccgtatttaa taaaaatatt tcgtgagtct ctgtgcgcta
     3481 attctccatc tggcgtaggg aaaatcacat ctgaatcagg aattaacaat gaaacctgta
     3541 aaaccacctc gtattaatgg acgagtgccg gtcctgtcgg cacaggaagc ggtgaattat
     3601 attcccgacg aagcaacact ttgtgtgtta ggcgctggcg gcggtattct ggaagccacc
     3661 acgttaatta cagctcttgc tgataaatat aaacagactc aaacgccacg taatttatcg
     3721 attattagtc cgacagggct tggcgatcgc gccgaccgtg gtattagtcc tctggcgcaa
     3781 gaaggtctgg tgaaatgggc attatgtggt cactggggac aatcgccgcg tatttctgaa
     3841 ctcgcagaac aaaataaaat tattgcttat aactatccac aaggtgtact tacacaaacc
     3901 ttacgcgccg ccgcagccca ccagcctggt attattagtg atattggcat cgggacattt
     3961 gtcgatccac gccagcaagg cggcaaactg aatgaagtca cgaaagaaga cctgattaaa
     4021 ctggtcgagt ttgataacaa agaatatctc tattacaaag cgattgcgcc agatatcgcc
     4081 ttcattc
//
LOCUS       DAJTYZ010000232         4077 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000232 DAJTYZ010000000
VERSION     DAJTYZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.234"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..784
                     /locus_tag="OO596_004049"
     CDS             <1..784
                     /locus_tag="OO596_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6719743.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1757
                     /locus_tag="OO596_004050"
                     /pseudo
     CDS             797..1757
                     /locus_tag="OO596_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="OO596_004051"
     CDS             complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="OO596_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6719744.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="OO596_004052"
     CDS             complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="OO596_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6719745.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1225 a          954 c          861 g         1037 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaaatagag cttccaggaa ggtcatctta taatttagaa gaaatgctat
     1141 ctgcgataaa tttcaccatt ccatactcta tcgcatctgt atcagatagt tccaacaccg
     1201 cagatatttt tccaggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta
     1261 cagggaataa tactaattgt aatagcgtcc gctttaatta taatttaaat actattttac
     1321 aggtaaattt aatgacctgt ggttttgaag atcaatccat cgacactgga cgatttgtac
     1381 tgtctcgtat caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg
     1441 gcgagcaagg tgaattagtt tttgaaccca gtgatgttga atattacttt gaaccagtga
     1501 cgatacagga gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg
     1561 cgggtcaagt aggctttcaa ccctccaatg atggtacgaa tgaaatccag tatggtaaaa
     1621 gcaattacta ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc
     1681 gcccgcgtac atatggaaat aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg
     1741 tggttatgta caactaatct atctccccca ccttaacttt gggggaggtg ccgattaacg
     1801 cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     1861 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     1921 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     1981 gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag
     2041 gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga
     2101 acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg atttttctgg
     2161 tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     2221 ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact
     2281 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc
     2341 ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac
     2401 gccaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg
     2461 gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc
     2521 gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat
     2581 ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc
     2641 ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     2701 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     2761 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     2821 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     2881 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     2941 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     3001 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     3061 ggcagaatgg aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc agcattagca
     3121 aatgcaaaca gcggtaaaat cagatacgcc acccacgggt gcaatacatg ctccagtcgc
     3181 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     3241 gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacaccac gccaacaaga
     3301 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct
     3361 acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     3421 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagtgctaac
     3481 ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     3541 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     3601 gccggaacaa tcatcccacc aatagcggcg ataaccggaa atgcggcctg gcgtaagctg
     3661 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     3721 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     3781 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctgttg
     3841 gccatcatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     3901 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     3961 tactatttta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt
     4021 tttttacgag caattaaagg atttgcgcca tcagcggtga gatgagtttt tgacggc
//
LOCUS       DAJTYZ010000233         4049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000233 DAJTYZ010000000
VERSION     DAJTYZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.235"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            25..1171
                     /locus_tag="OO596_004053"
                     /pseudo
     CDS             25..1171
                     /locus_tag="OO596_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1465..2706
                     /gene="agp"
                     /locus_tag="OO596_004054"
     CDS             1465..2706
                     /gene="agp"
                     /locus_tag="OO596_004054"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6719746.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2744..2971)
                     /locus_tag="OO596_004055"
     CDS             complement(2744..2971)
                     /locus_tag="OO596_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6719747.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(2992..3597)
                     /gene="wrbA"
                     /locus_tag="OO596_004056"
     CDS             complement(2992..3597)
                     /gene="wrbA"
                     /locus_tag="OO596_004056"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6719748.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3649..>4049
                     /locus_tag="OO596_004057"
     CDS             3649..>4049
                     /locus_tag="OO596_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6719749.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIGTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
BASE COUNT         1142 a          863 c          954 g         1090 t
ORIGIN      
        1 tattaaattt tatagacgct atatatgggt agtaatatac atggaattag ttgcactgca
       61 aataattatt tgaaacaggc ctggaacgat ataaaaaatg agtacgaaaa aaatcaaaca
      121 tattcaatca cgctttttga aaacacactg gtgtgtttta tgcggttata caatgaactc
      181 agacgtaaag taaatgaaga ggatactcca tgtctggaat gtgaatcact agaaaaagaa
      241 tttgaggaaa tgcagaatga taatgatcta tcattattta tgagaacatt gcgtactaat
      301 gatacacaaa tttattcagg ggtttcagga ggtattacat atactataca atatgttcaa
      361 aatgttgata ttgttagagt gtccttgccg ggcagaggtt cagagtctat cacagatttt
      421 aaaggttatt attggtatgg ctttatggaa tatattgaaa acattactgc gtgtgatgat
      481 attttttctg agtattgttt agatgatgaa aatatgagta tccagccaga acagataaat
      541 acgccgggaa tatctgattt agatactggt tttaaaagaa catgagtatt catgggcgct
      601 gggcggccat aagacgcaaa agcaagtata cgaagagttt attaaaattg ttgataaaca
      661 aacgatcgag gctcttgttg atacggcatt ctataatctc agggaagatg taaagacgtt
      721 atttggcgtt aatctacaat ctgacaacaa aattcagggg cagggtggtt ttttatcatg
      781 gagttttttg tcttttaaaa aagaatttat tgatagttgc ttgaatgaca aaaaatgcat
      841 actgcattta cccgaattta tatttaatga taacaaggca cgccttgttt tagacaccga
      901 cccagagcag aaggttaatg aggtgaaaaa tttcctcact gctctttcag atagcatttg
      961 ctcattattt attgtttaca gcaatgtggc atcaatctcc ttagggaatg aatccttttc
     1021 aacagatgac gaccttgagt atgggtattt aataaacact ggcaatcatt atgacgttta
     1081 cctccctcct gaactttttg ctcaggctta cgagttaaac aataaggaaa ggaatgcgca
     1141 aattgatttt ttaactcgtt atgcaattta atgacaaagg catgtgctaa aaaccattgt
     1201 tattagcctc acttctctta ttattggtac tattgcctct gtattagtaa cgccgctgat
     1261 attctgttta tccacaggtg caattatcag cggcgtacgc gaggcagggg ctaatcaggc
     1321 atagtttgcg tcaaaccttg cctgtttttg aagatgtata tagaaaaaca ggcgttcaac
     1381 aagccatttt gcgaacctgt tcccggaaaa aagtcatatt tctgtcacac tctttaatga
     1441 ttgataacaa aagaggtgcc aggaatgaac aaaacgctaa tcgccgcaac tgtggcaggg
     1501 atagttttac tcgcttcaaa tgctcaggca caaaccgtac cggaaggcta tcagctacag
     1561 caagtgctca tgatgagccg ccataactta cgtgcgccgc tggcgaacaa tggcagtgtg
     1621 ctggagcagt cgacgccgaa taaatggcca gaatgggacg tccccggtgg gcaactcacc
     1681 accaaaggtg gcgtgctcga agtgtatatg ggccattaca tgcgtgaatg gctggcagag
     1741 caggggatgg tgaaatcggg ggaatgcccg ccgccggaca ccgtttatgc ctatgccaat
     1801 agtctgcaac gtaccgttgc gaccgcacag ttctttatta ccggcgcatt cccggggtgt
     1861 gatattcctg tgcatcacca ggaaaaaatg ggcactatgg acccaacctt taacccggtg
     1921 atcaccgatg attccgccgc attcagtgaa caggcggtgg cggcaatgga gaaagagctc
     1981 agcaaactcc agcttaccga cagctaccag ctactggaaa aaatcgttaa ctataaagat
     2041 tcccctgcct gtaaagagaa acaacagtgt tcgctggtgg atggcaaaaa tacctttagc
     2101 gccaagtatc aacaagaacc aggtgtttcc gggccgctga aagtcggcaa ctcgctggta
     2161 gatgcgttta ctttgcaata ttacgaaggt tttccgatgg atcaggtggc ctggggagaa
     2221 atcaaatctg accagcagtg gaaggtgttg tcgaagctta aaaacggcta ccaggacagc
     2281 ctgtttacct caccggaagt ggcgcgcaat gttgcgaaac cgctggtcag ttatatcgac
     2341 aaagctctgg tcaccgatcg caccagcgca ccgaaaatta cagtgttggt tgggcacgac
     2401 tccaacattg cctctctgtt aacggcgctg gatttcaaac cgtatcagtt gcatgaccag
     2461 aacgaacgca cgccgattgg cggcaaaatc gttttccagc gttggcatga cagcaaagcc
     2521 aatcgcgatt tgatgaaaat tgaatatgtg tatcagagtg cggaacagtt acgtaatgcc
     2581 gatgcgttaa ccctgcaggc acctgctcag cgtgtgacgc tggaattaag cggttgcccg
     2641 atagatgcca acggtttctg cccgatggat aaatttgata gcgtgttgaa tgaagcggtg
     2701 aaataacaga aaactccccc gcgagaagcg ggggagtcgc tggttaaacg tttttacgtt
     2761 cgatggtctg ttcgccccaa aaaagcgaat ctttatcggc cttagcaaag gctttgacta
     2821 acacttcatc gctaccttct tcccaaatct tttctgccat tttttcgtcg tacccggcga
     2881 cttcgaaaat ggcctcggct atttccggcg acgtattgcg cagagatgcc cattcaccga
     2941 cgtgatgagc tttcgcttct tgagttggca tgcgtatcct cctgttgaag attagccgtt
     3001 aagtttaact gccagacctg cgacatattc cccttgataa cgagcaatag acagttcttc
     3061 ctggcttggc tggcgtgagc cgtcaccgcc tgcgatggtg gttgcgccgt acggcgtacc
     3121 gccgcgaacc tgtgaaacgt caaataattc ctgcgctgcg tagccaatgg ggacaatcac
     3181 catgccgtga tgcgcaaggg tcgtccaggt tgaagtaata gtttgttcct gaccgccgcc
     3241 agtaccggtg gaactaaaga cgctcgccag ttttccgtat agtgcgccgg aagcccacag
     3301 gccgcccgtc tggtcgagga aggtacgcat ttgaccggac atgttgccaa agcgggtagg
     3361 tgtaccaaaa ataatggcgt cgtaatcggc cagttcttgc ggggttgcaa ccggtgcagt
     3421 ttgcgtttta ccgcctgctt tttcaaataa ttgcggcggc atggtttccg gtacacgctt
     3481 aacgacaact tctgcgccat cgactttgct tgcaccctca gcgactgcgc gtgccatcgt
     3541 ttcaatatgt ccgtacatgg aataataaag cactagtatt cttgccttaa cactcatatc
     3601 attgttctcc tgttcaatga accacttaga taagcatagt ccgaagatat gaagctgcta
     3661 gtgacataca tcaggtggag tactaaagag caagactcag gagactcctt aagacggcaa
     3721 acaaacctca ttgatgcttt ctactctaag cacaaaaacg attattacct tcttcctgct
     3781 cacagatatg ttgataaggg taaatctggt tttcaccagc aacataagaa tcaaggttct
     3841 gacttcagac gtatgtttga aaatgttatg tctggggcta ttccagaagg ttccttgatt
     3901 gttgtagaga actttgaccg cttcagtagg gctgatatcg gcacagctat agatgatgtc
     3961 aggcagatac tgaggaaggg ggtctctata ctgactctgg gtgatggtga actctacgac
     4021 aagtcagcct taacagaccc tgtgaagtt
//
LOCUS       DAJTYZ010000234         4000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000234 DAJTYZ010000000
VERSION     DAJTYZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.236"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            234..1898
                     /gene="tssM"
                     /locus_tag="OO596_004058"
                     /pseudo
     CDS             234..1898
                     /gene="tssM"
                     /locus_tag="OO596_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3261
                     /locus_tag="OO596_004059"
     CDS             1909..3261
                     /locus_tag="OO596_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCR6719750.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3285..3767
                     /locus_tag="OO596_004060"
     CDS             3285..3767
                     /locus_tag="OO596_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCR6719751.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            3811..>4000
                     /locus_tag="OO596_004061"
     CDS             3811..>4000
                     /locus_tag="OO596_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719752.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDV"
BASE COUNT         1074 a         1029 c         1069 g          828 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaaca
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
     3961 cagaacactc atcatgttat tctcttgaca cttgatgttg
//
LOCUS       DAJTYZ010000235         3978 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000235 DAJTYZ010000000
VERSION     DAJTYZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.237"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            171..506
                     /locus_tag="OO596_004062"
     CDS             171..506
                     /locus_tag="OO596_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6719753.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            499..981
                     /locus_tag="OO596_004063"
     CDS             499..981
                     /locus_tag="OO596_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719754.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1015..1095)
                     /locus_tag="OO596_004064"
     CDS             complement(1015..1095)
                     /locus_tag="OO596_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719755.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1131..1555)
                     /locus_tag="OO596_004065"
                     /pseudo
     CDS             complement(1131..1555)
                     /locus_tag="OO596_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1985..2475
                     /locus_tag="OO596_004066"
                     /pseudo
     CDS             1985..2475
                     /locus_tag="OO596_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2700..3191
                     /locus_tag="OO596_004067"
     CDS             2700..3191
                     /locus_tag="OO596_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6719756.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3526..>3978
                     /gene="ygeH"
                     /locus_tag="OO596_004068"
     CDS             3526..>3978
                     /gene="ygeH"
                     /locus_tag="OO596_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HCR6719757.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKID"
BASE COUNT         1491 a          635 c          640 g         1212 t
ORIGIN      
        1 agatccataa tgacattaac atacaaaatc atgtaataaa ctcgtcaaca gacgcacata
       61 cacacaatgc cccacctgcc ataaaagtta atgcgggata caaagagagc attggtggcg
      121 caaagaattt caataacaaa atcctcaaac atataaaaag tcatctaatt atgttgagcg
      181 catttgtcat aggcgcatat tctgcatatt ggctatggaa taataatcaa ccgaagccat
      241 ttttcaagga ttacaagact gttgcggaaa ttaatggctg ccattttaat gtaacagaag
      301 atacaattga tgggttgaaa gagttcgata aatataaaac acggatactg gattcgggga
      361 ttaactgtaa aaaatatccg tggttatact tccctcttgc aaaatcctca ccggggatga
      421 ttgttatggc atgcaataaa aactataacc aacatgaagt ggcagactgt ttaaccctgt
      481 cttaccgtga ggttaatcgt gattgattat aaaaaaaact tattatttat tttagttttt
      541 atcagtgggt ttattctctt cactgtctat tcttacactg cagaaaaaat gatatataac
      601 gaaacatgca ctgcaaattg ggtaattttt aatgaccagg gacgagcaaa tcttaccatc
      661 gactttatgt ataacaaaaa aaataaaaca ggaactgttg cgttgagtgg aacctggcaa
      721 caaggtaaca gagaaagtaa atcaatacgg cgaaatattg aatacacatg gattgaaaac
      781 tatgacacag cccatttaac atcaaagaaa gttaataagt ttgagattat ggatcaagtt
      841 gatgatgata gacttgcaca acttattcct gatttttatg tctttccaga aaaaagtgta
      901 agctataata tactaaagca aggtaagcat gcttttattt tgagcattgg taacagagca
      961 ataatgcatt gtgcaaggta aactaaaata gataacaaaa aagatggaac ctcgttaatc
     1021 ataaaacaat aaaattaaat attctcgcag tatatggcag tctaaagcat caaagttttg
     1081 atcaacatct ttcattttag acatctcctt gcaatgtaaa atatcatgta tcaacaacat
     1141 ctggtgcaaa tccatgagtc gaactcgaca ttcatcttag ctcgattatt attccttcgt
     1201 acagtcgatg taaacaatac agaaagagga ttattaagaa ccagtttact caggaatcaa
     1261 tagaggaaag gactaacgtt tctttaaaag aattgatttc atcatctgtt aaactaaact
     1321 catcattgac agatcgtgag atataactgt ttttaacttt actctttacg ttgactttat
     1381 tgacagaatt aacattcaca tatcttgaat ttaatgtcca tgatgttgtt tgcgtgagaa
     1441 cattctcagc attaaggaat ttttttaacg gaatccttgg tttctttttc gagtccgaat
     1501 ttacaatatc atgcatatag accatttcat gaatctgcga aaactcaatg tccattgtat
     1561 acctcacatt tttaccgtga ctcgatgtta ctgttcaata atcaccttcc atcaatacta
     1621 aaattaatac ccctaatgtg ccgataacaa atatagtcat tctacgtaac gtctccataa
     1681 ggtgataacc ttgatcaaat gaatttcgaa tataataaag gaaatctttt gagatgttta
     1741 tcacgaataa agctatttat gttaccaata tcatttcgag tgcgcacact atctatctgt
     1801 tttttcgaga tataaaaaac aacgtgtaag ttatgtaaat tgccgatgct actgagtaac
     1861 attatggtaa aaaaagtaaa aatgagcgat ttttgataga ataaagcatg agaaaaggtg
     1921 atatttgaca ttatcagaag ctgcgaattc ggattttgct ctaatcaaat gagagaaatg
     1981 taaaatgaag ccacgaaata ttaataatag cctaccactg caaccattag ttcctgatca
     2041 ggagaacaaa aataagaaaa atgaagagaa atccgttaat ccagttaaaa tcacaatggg
     2101 gtctggttta aattatattg aacaagaatc tcttggagga aaatatctaa cacatgattt
     2161 gtcaataaag atagcggata tttctgaaga gataattcag caagcaatat tatctgctat
     2221 gagcatatat aaattttcga taacagatga tttaatgagt atggctgtaa atgaactcat
     2281 aaaactgacc aaaatagaga ataatgtaga cctgaataaa ttcactacta tatgcacaga
     2341 cgttctatcc cccctcgtca ccagacataa taaagaaaaa aaacaaacga cattctaccc
     2401 ttctcaaaaa tccccttttt aattttcatt gaaaaaatgg caattgatac tatgaatcca
     2461 ggtcatacac attaaggcat ttatgtaatg tcgtaattaa gataactaat aaggtgaata
     2521 ttagtaaaac aactaatgat gttattagtt tgtagtgaac agtactttta ccaataatga
     2581 aaaatatacc ataagcaatg caagcttaaa actaataatt taaaaaagat aaatgtaaaa
     2641 atattaatga gtcaatcaat aataatttag catcacgaga cacatcacag aggaatatta
     2701 tgagcacaga aacaattgaa atattcaata atagtgatga atgggcaaat caactaaaac
     2761 acgcattatc gaaaggagaa aatctggcat tactacatgg tttaactcct gatatccttg
     2821 atagaatata tgcatatgca ttcgactacc atgaaaaggg taatataacg gacgcagaaa
     2881 tttattataa atttttgtgc atttatgcgt tcgaaaatca tgagtatcta aaagattttg
     2941 catcagtatg tcagccaaaa aagaaatatc aacaagcata tgacctttac aaactaagtt
     3001 acaattactc cccgtatgat gactattcag ttatttatcg tatgggtcaa tgtcagattg
     3061 gggctaaaaa tatcgataac gcaatgcaat gtttctatca cattattaac aattgtgagg
     3121 atgatagtgt taagagtaaa gcgcaggcat atattgaact cttaaacgat aattcagaag
     3181 ataatggcta agcattacaa tcatttggtt aaatttgctg gttatagtgc agttaactac
     3241 tataaccagc acctcttatg ttttcttaaa tcagcatctt aaaacagtta agagcatact
     3301 ataaatcatt atttttcaat gcgttagaga acaatctact ttataaaaat tgaataaggc
     3361 tatttcagat tacatcactg aattcctaag ataaattagt gaacacaacc atagatatgt
     3421 cgaatgtatt ttcctgtagc attaatgaca cataaatatg aatagccata atttctattg
     3481 cttaagcagg atgcaagaaa ccaatttttt catagaggtt aactaatgga cttagaaaat
     3541 aaattctcat atcattttct tgagggatta acgctcacgg aagatggaat tcttactcaa
     3601 ggaaatgagc aagtttatat tccacagaaa gagttaggtg tattaatagt attacttgaa
     3661 tccgctggtc atgttgtact gaaagatatg atcatcgaat cagtatggaa aaatattatt
     3721 gttagtgacg agtccctgac aagatgtatc tattctttgc gctgcatttt tgaaaaaatt
     3781 ggctatgatc gttgcataga aacaatctac cggaaaggtt atcgtttcag cgggcaggtt
     3841 ttcaaaacta aaattaatga agataatact tcagactatt ccatagctat attccctttc
     3901 actacttcat tgaatacact ggatccatta atacttaatc aggaattagt gcaaatcatt
     3961 tcaaataaaa aaatcgat
//
LOCUS       DAJTYZ010000236         3962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000236 DAJTYZ010000000
VERSION     DAJTYZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.238"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..996
                     /locus_tag="OO596_004069"
     CDS             <1..996
                     /locus_tag="OO596_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCR6719758.1"
                     /translation="MAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDV
                     FEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYV
                     ALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLA
                     DNMQDGVRIARDNPDSGVVVSIAGEGRPWNPGAITDGRVWGDIPDNSVQPGAGNGEPV
                     TAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTELAVREIAGQERDRAAI
                     TEREAALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKEKTPGGD"
     gene            1016..1763
                     /gene="traX"
                     /locus_tag="OO596_004070"
                     /pseudo
     CDS             1016..1763
                     /gene="traX"
                     /locus_tag="OO596_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            1818..2378
                     /locus_tag="OO596_004071"
     CDS             1818..2378
                     /locus_tag="OO596_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6719759.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            2508..2720
                     /locus_tag="OO596_004072"
     CDS             2508..2720
                     /locus_tag="OO596_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6719760.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            2964..3425
                     /locus_tag="OO596_004073"
     CDS             2964..3425
                     /locus_tag="OO596_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6719761.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWMHRK"
     gene            3471..3680
                     /gene="hha"
                     /locus_tag="OO596_004074"
     CDS             3471..3680
                     /gene="hha"
                     /locus_tag="OO596_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6719762.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            3719..>3962
                     /locus_tag="OO596_004075"
     CDS             3719..>3962
                     /locus_tag="OO596_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719763.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKR"
BASE COUNT         1006 a          909 c         1204 g          843 t
ORIGIN      
        1 atggccggct ttgagtcagc ctacgtggcc ctgtcgcgta tgaagcagca tgtgcaggtg
       61 tacaccgata accgtcaggg ctggacggat gccatcaaca atgccgtaca gaaaggaact
      121 gcccacgatg tgtttgagcc gaaaccggac cgggaggtca tgaatgcaga gcggctgttc
      181 agtacggcgc gggagctgcg ggacgtggcg gcaggccgtg ccgttctccg tcaggcgggg
      241 ctggccgggg gagacagtcc tgcacggttt attgctccgg gacgtaaata tccgcaaccg
      301 tatgtggcac tgccggcgtt tgaccgtaac ggcaagtccg ccggtatctg gctgaacccg
      361 ctgaccacgg atgacggaaa cgggctgcgg ggattcagcg gtgaaggtcg ggtaaaaggc
      421 agcggggatg cgcagttcgt ggccctgcag ggcagccgta acggagaaag cctgctggct
      481 gataatatgc aggatggcgt ccggattgcc cgtgataatc ctgacagtgg tgtggtggta
      541 agcattgccg gtgaaggccg tccgtggaat cccggtgcca tcaccgatgg ccgcgtgtgg
      601 ggggatatcc cggacaacag cgtccagccg ggagccggaa atggcgaacc ggtcacggca
      661 gaggtactgg cacagcggca ggctgaagag gccatccgcc gtgaaacgga acgtcgcgca
      721 gatgaaattg tccgtaaaat ggcagagaac aaacctgacc tgccggacgg caaaacagag
      781 ctggctgtca gggaaattgc cgggcaggag cgtgaccggg ctgccataac tgaacgggaa
      841 gccgcgctgc cggagagtgt gctgcgtgaa tcacaacggg agcgggaagc ggtccgggag
      901 gttgcccggg aaaatctgct gcaggagcgt ctgcagcaga tggagcggga tatggttcgt
      961 gacctgcaga aagagaaaac cccgggcgga gactgataca ggaagacaaa cactgatgac
     1021 aacggataac acgaacacga cacgtaacga ttcactggct gcacggaccg atacctggtt
     1081 gcagtcattt ctggtctggt cacccggaca gcgggatatc atcaaaacgg tggcactggt
     1141 gctgatggta ctggaccaca tcaatctgat attccagctg aagcaggaat ggatgtttct
     1201 ggccgggcga ggagcctttc cgctttttgc cctggtgtgg gggctgaatc tgtcccgtca
     1261 tgcgcatatc cggcaaccgg ccattaaccg gctgtgggga tggggaatta ttgcccagtt
     1321 cgcatattat ctggcaggct ttccctggta tgaggggaat atcctgtttg cctttgcagt
     1381 ggcggcacag gtgctgacgt ggtgtgaaac gcgcagtggg tggcgtacag cagcggccat
     1441 tcttctgatg gccctgttgg gggcctttgt ccggcaccag ttacggcatt gccgggctgc
     1501 tgatgctggc agtcagccac cgactgtacc gggcggaaga cagagcggaa cgtctggcgc
     1561 tggtcgcctg cctgctggct gttattccgg cgcttaacct tgccaccagt gatgcagcgg
     1621 cggtggccgg tcttgtgatg acggtgctga ccgttggtct ggtgtcgtgt gcaggaaaat
     1681 cattaccccg tttctggccc ggggattttt tcccggtatt ctacgcctgt catctggctg
     1741 tgctgggcgt tctggcgctg tgacgggtgt ggtatctttg accgtaacag gatgattcgt
     1801 cagaggcaga acacagcatg acagagcaga agcgaccggt actgacactg aagcggaaaa
     1861 cggaagggga gacgcctgtc cgcagccgga aaaccatcat caatgtcacc acgccaccaa
     1921 agtggaaggt gaaaaagcag aagctggccg agaaagcagc ccgggaagca gagctggcgg
     1981 caaaaaaagc gcaggccaga caggcactgt ccatttatct gaacctgccc acgctggatg
     2041 aggccgtgaa taccctgaaa ccctggtggc cgggattatt tgacggtgac accccccggc
     2101 ttctggcctg cggtatccgg gacgtgttac tggaagacgt ggcgcagcgg aatatcccgc
     2161 tctcgcataa aaaactgcgc agggcgctga aggccatcac ccgttcagaa agctatctgt
     2221 gtgccatgaa agccggtgcc tgccggtatg acacggaagg gtatgtgacg gagcatattt
     2281 ctcaggagga ggaagcgtat gcggcagcgc gtctggataa aatccgccgc cagaaccgga
     2341 taaaggcaga acttcaggcc gtgcttgatg agaaataaaa aaaagcctcc atcagagagg
     2401 aggcagggaa ataaggctta aaaggaaata acttccgctt ccgaaaggaa gccagtccac
     2461 tgaattattt atcacaataa tactgacaac taaaggggga caagaaaatg aatggattca
     2521 gaaacagttc acggaacggc caggtctggc gttaccagcg tgccggcggg cgggccgtta
     2581 ttctggaagt cagtggacgc tggatggaag cggcagaagc atggcgacgg gctgcctgtg
     2641 ttgcgcccag gacagactgg cagcagtttg cccgaaaaag ggctgaacac tgccaccggc
     2701 gctgccgggg aagggtgtaa cgaaaaaagc agccttcatt actccaggag tcagaaggct
     2761 gaaaggagtt tatgagattt gcttcttaat atccggtcgg gcagtatgac cggacagttc
     2821 tgagtagcac agagtctttt ccgcattaag tcgatttttt atattttgtg attaatttca
     2881 caaaataagg tgttattcaa tgtgtgctgc cctattcagg ggggctgaaa tacaggtgcc
     2941 atttatctga tatggagaat aacatgagga aatatattcc actggtatta tttatctttt
     3001 catggccggt attgtctgca gatattcatg gacgggttgt tcgtgtactg gacggtgata
     3061 ccatcgaggt catggactca ctgaaggcgg tgcggatcag gctggtaaat attgatgcac
     3121 cggaaaagaa acaggattat ggccggtggt caacagacat gatgaaatca ctggtcgcag
     3181 gaaaaacggt taccgtcaca tattttcagc gagatcgtta tggtcgtatt ctcgggcagg
     3241 tttatgcacc agacggaatg aatattaatc agtttatggt ccgggccggg gctgcctggg
     3301 tatatgagca gtacaacaca gatcccgtgc tacctgtcct tcagaacgaa gccagacaac
     3361 aaaaacgtgg gctctggtca gatgctgatc cggttccccc ctggatatgg atgcaccgta
     3421 aataaagcga tggcaatcct atcattcatc tgttctcagc gggaataaaa atggcaaaaa
     3481 caaaacagga atggctgtat cagttacggc gatgttcatc agtcaatacg ctggaaaaaa
     3541 ttattcataa aaacagagac agtttatcaa acagtgaacg tgaatcattt aattcagcag
     3601 cagaccaccg gttagcggaa ttaatcaccg gaaagttata tgacaggata ccaaaagaga
     3661 tatggaaata tgtaaggtaa taattcagca ataattaatt gccaggagca gataaataat
     3721 gaagttgatt atttttattc tcattgttct gattattgca gcgctactta tccggattat
     3781 cctcagaagt gtaaatcagc attctccttt gctgatgcaa ttgcatgcgg caggtatacg
     3841 gaccggtgat gcagagcgaa tactgtccag cggtgaatac tggcaacgtc agaagacgct
     3901 gctgacagaa agggaagtca gttttatgaa aggactgttc agaattgtgg atatgaagcg
     3961 gt
//
LOCUS       DAJTYZ010000237         3961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000237 DAJTYZ010000000
VERSION     DAJTYZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.239"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..242)
                     /locus_tag="OO596_004076"
     CDS             complement(<1..242)
                     /locus_tag="OO596_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6719764.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OO596_004077"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OO596_004077"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6719765.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIANLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="OO596_004078"
     CDS             2255..3904
                     /locus_tag="OO596_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6719766.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1033 a         1000 c          928 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagattagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 g
//
LOCUS       DAJTYZ010000238         3956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000238 DAJTYZ010000000
VERSION     DAJTYZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.240"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..663
                     /locus_tag="OO596_004079"
                     /pseudo
     CDS             1..663
                     /locus_tag="OO596_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            670..1416
                     /locus_tag="OO596_004080"
     CDS             670..1416
                     /locus_tag="OO596_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6719767.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1431..1853
                     /locus_tag="OO596_004081"
     CDS             1431..1853
                     /locus_tag="OO596_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6719768.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1911..2267
                     /locus_tag="OO596_004082"
     CDS             1911..2267
                     /locus_tag="OO596_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719769.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2436..3101
                     /locus_tag="OO596_004083"
     CDS             2436..3101
                     /locus_tag="OO596_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6719770.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            3329..3484
                     /gene="hokD"
                     /locus_tag="OO596_004084"
     CDS             3329..3484
                     /gene="hokD"
                     /locus_tag="OO596_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6719771.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            3734..>3956
                     /locus_tag="OO596_004085"
     CDS             3734..>3956
                     /locus_tag="OO596_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719772.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEI"
BASE COUNT         1079 a          869 c         1097 g          911 t
ORIGIN      
        1 gggcatgggc ggatcaacaa atgatagacg gtaatgcaga ttgtaacgct cgcggcgtta
       61 caaaaagtgc aatagatcgc atcactttta tgtctggttt tgctgatgcg ttaattcagg
      121 ttggatggct ggtcgaaaat gacggagggc tttctctacc taactttgaa cgtcataacg
      181 gaaaaagctc taaaaaaaac gggcggttac aaacgagcga gtaacaaaaa tacgcgaact
      241 gaaacgaaaa ggtaacgctg ccagcgttac acaaacggat caaaaagcgt taccagagga
      301 agaggaagag gaagatctaa atactgatct ccccctcgcc aaaaacgagc gtctaaaaaa
      361 ttcgagccgg aggctattga gctgcccgat tggttgccgg aaacactctg gcatgagtgg
      421 gtccggttca gacaggcatt gcgaaaaccg attcgaacgg agcagggcgc taacggggcg
      481 atacgggaac tggaaaaatt ccgtcagcag ggttttacac ctgagcaggt gattcgacac
      541 agcatcgcca atgaatacca gggcctgttc gcgccgaaag gtgttcggcc tgagacgttg
      601 ctccgacagg ttaacaccgt ctcgttgccg gacagtgcga tcccgccagg cttcaggggg
      661 taacggacca tgaaaaatat tgcgacaggc ggcgttctgg aacgcatccg ccgactgacc
      721 ccgccacatg taaccgcccc attcagaacg gtagcggagt ggcgcgagtg gcaacttgct
      781 gaaggccaga aacgtagcga ggagatcaac cgcctgaatc gccagttgcg ggtggaaaaa
      841 attctgaatc gctcaggcat ccagccgttg caccgtaaat gctcgtttgc gaattaccag
      901 gtgcagaacg acggccagcg atacgcgtta agccaggcga aatccatcgc cgatgaactg
      961 atgaccgggt gtacaaattt tgcgttcagc ggaaaacctg gtaccgggaa gaatcactta
     1021 gcggcagcta tcgggaatcg cctgctgaaa gacggtcaga cagtgattgt ggttaccgtg
     1081 gctgatgtta tgagcgccct gcacgccagc tatgacgacg ggcagtcagg cgaaaaattt
     1141 ttgcgggagc tgtgcgaagt ggatctgctg gttcttgatg aaattggcat tcagcgcgag
     1201 acgaaaaacg agcaggtggt gctgcaccag attgttgatc gccggacagc gtcgatgcgc
     1261 agcgtgggga tgctgacaaa cctgaactat gaggtgatga aaacattgct cggcgagcgg
     1321 gtgatggatc gcatggtcat gaacggcggg cgctgggtga attttaactg ggagagctgg
     1381 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
     1441 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
     1501 acggtcgggt cacgattcgg cagttgtcag atgaaacagg tatcagtcgt gcgtctgtcg
     1561 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
     1621 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
     1681 aggtgaggaa accggttgtt gttgatccgg accttatctg gtcattacct gacggagaaa
     1741 tacgccgcta cgacaggcgc ctgaatataa tctgtcgcga gtgccggaag agcgaagcta
     1801 tgcagcgtgt actggctttc tatcagggta attttcagga ggcgatactg tgaatgaaat
     1861 tagctatcag gcttcaatta ccgctggcat tcgcatcaaa ggagaggagc atggaaataa
     1921 aaccagaaga tgagttaagc aatatcgttt tatttccggt aaaagaggat gaccctcgta
     1981 atcaggttaa ttttctttat gagccatcgg aaagaccata ttgccatcac gcctctgtac
     2041 gggttgacga aaaagagcgt caggtccgct gtaaaatctg cggtgcggtt gtggagccgt
     2101 ttgactggat gctctctgtg gcaaaaagag aaaccagact ggcagatgat gtaaggctat
     2161 tgcgccagga ggaacaggaa aggcggagaa atatagaaaa gctgatacag attgagcgta
     2221 acgcgaaagc gcggatacgc agggcgacaa aatccagaac tgaataatta aatttagcac
     2281 tgttaaaaat ttaatcctta accggaggga tttctgcacc ctcaaatcat caggagacca
     2341 cccgaaaggg cggggagcag tcacacatct gtttccgata gccccgttct aatgctacac
     2401 tctttgatat ttttatgacc ccaataaaca tatttatgac agttgctgat ttcaaacggc
     2461 ccaaattgga gctcccaaac ggggcaaaca aactactact gcactcttgc tgtgctccat
     2521 gttccggtga ggtgatggag gcgcttcagg cctcgggaat cgactacacc atctttttct
     2581 acaacccgaa cattcatcct cagaaagagt atttaattcg taaggatgag aatattcgct
     2641 ttgctgaaca acacggcgtg ccgtttattg atgctgatta cgacacagac aactggtttg
     2701 aacgagccaa aggaatggaa tgggagcccg aacgagggat ccgttgcacc atgtgttttg
     2761 acatgcgttt tgagcggaca gcgctgtacg ccgctgaaaa tggtttcagt gtgatcagca
     2821 gttcactggg catttcacgc tggaaaaata tgcagcaggt taacgactgt gggcggcgag
     2881 ccgtcgcgca ttatccgggc atggtgtact gggattataa ctggcgcaag cagggcggct
     2941 cgtcccgtat gattgaaatc agcaagcgcg aaaaattcta tcagcaggaa tattgtggct
     3001 gtgtgtattc tctgcgcgat accaatctac accgcaaatc tcagggacgc cctcttatca
     3061 aaattggtca actccactac ggtaaagaag agaaggagtg attttatggg gcacctttct
     3121 gattgatttc atattggcga ggtaagtaga atgactgcgg gtgcttgagg ctatctgctt
     3181 caggcatgaa caccaaaagg cagatagaga aaagccccag ttaacattac gcgtccggca
     3241 agacgcttaa cattaatctg aggccaattt catgctttgc acatgtaggt tagcctctta
     3301 catgccgaaa ggcaaggaga agcaggctat gaagcagcaa aaggcgatgt taatcgccct
     3361 gatcgtcatc tgtttaaccg tcatagtgac ggcactggta acgaggaaag acctctgtga
     3421 ggtacgaatc cgaaccggcc agacggaggt cgctgtcttc gtagactacg aatccaggaa
     3481 gtaagagtga ccgggcgggg agctgatccc atccccgccc acctctgatg tgtcaggcat
     3541 cctcaacgca cccgcactta acccgcccat cgctgtgatc tctcagcgtt tcggcgggtt
     3601 ttttgttgtt tatttccggt gaatttgatt cgcgcacctt cgcagataga atcgactcac
     3661 ttaagtagcg cgcagggaga agagggatgg accccgaaca ggggagagct atttatctgg
     3721 aaggattctg aagatgaaaa tcgaagaatt gcgtgaaatt tttagtgaaa atggcctcta
     3781 tgctgtgcgc gttgagaatg gagcgattgt cagccattgc cgcattagat gtttgcaatc
     3841 tcaacaaagg aagagcggtg ctgtgttatt ttatttttgt aatggacttc tgacggacgg
     3901 ttttattttg cgtgaggacg aatttgtcac atcattacgg gttttgaaag agattg
//
LOCUS       DAJTYZ010000239         3934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000239 DAJTYZ010000000
VERSION     DAJTYZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.241"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..206
                     /locus_tag="OO596_004086"
     CDS             <1..206
                     /locus_tag="OO596_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCR6719773.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
     gene            345..1295
                     /locus_tag="OO596_004087"
     CDS             345..1295
                     /locus_tag="OO596_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6719774.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            1522..2970
                     /gene="uxaB"
                     /locus_tag="OO596_004088"
     CDS             1522..2970
                     /gene="uxaB"
                     /locus_tag="OO596_004088"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6719775.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3142..3450)
                     /locus_tag="OO596_004089"
     CDS             complement(3142..3450)
                     /locus_tag="OO596_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6719776.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(3444..>3934)
                     /locus_tag="OO596_004090"
     CDS             complement(3444..>3934)
                     /locus_tag="OO596_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6719777.1"
                     /translation="SGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNIK
                     FNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIAA
                     QNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQEA
                     SC"
BASE COUNT          951 a          932 c         1007 g         1044 t
ORIGIN      
        1 cggcattacc ggctaatgac ggtaagcaaa cccccatgcg cgggcatccg gtgtttatcg
       61 ctcagcacgc caccgcgacc tgctgtcgcg gttgtctggc taagtggcat aacattccgc
      121 agggtgtttc gttaagcgaa gaacaacaac gttatatcgt ggcggtaatc tatcactggc
      181 tggttgtaca gatgaatcag ccatgagaat tgcggaaaac gataaaatag cattcccagg
      241 gtacctttat ttgccgccgc tgttagcgaa catgctatcc tttatgagaa tttacttatt
      301 acgttgagat aattgccacc cgcaattgta tggacagata attaatgcat gtacacccca
      361 tttcaacttt ccgtttgttc caggaaggtc atctgctacg taatagcatc gctatttttg
      421 cgctaaccac gctgttctat tttattggtg cagagttacg gctggttcac gaactttctc
      481 ttttctggcc gctgaatggc gtaatggcgg gggggtttgc ccgctatgtc tggcttaatc
      541 gactgcatta ctatgcgatc agttatgtgg cgatgctggt ttatgatgcc ataaccaccg
      601 aatggggact ggtttcactg gttatcaatt tctccaatat gatgtttatt gttaccgtcg
      661 ccttactggt cgtgcgggat aagcgtctgg ggaaaaataa gtatgagcca gtaagtgcct
      721 tacggctatt taattactgt ctgattgccg cattattatg cgctattgtc ggggcgattg
      781 gttcggtcag tattgatagt ctggatttct ggcctttgct tgcggactgg ttcagtgagc
      841 aattctcaac gggcgtgttg atcgtgcctc tgacggtcat gctgcttgat atcgactatt
      901 tcaaaagcat taacgataac tatggacatg aatgtggcga taaagtgtta agcgtgtttg
      961 cccggcatat tcagaagatt gtcggtgata aggggctggt ggcgcgcatg ggcggcgaag
     1021 aatttgctgt tgcagtgccg tcggtgaatc ctgtcgatgg tctgctaatg gcggaaaaaa
     1081 tccgtaaagg cgttgaactg caaccattca cctggcaaca aaaaacgctt tatctcacgg
     1141 taagtattgg cgtcggtagt ggtcgcgcat cgtaccgaac gctgaccgat gattttaata
     1201 aattgatggt cgaagccgat acgtgtctgt atcgctcgaa gaaagatggg cgcaaccgta
     1261 ccagcactat gcgttacggt gaggaagttg tctgacatct ttattcattc cgcgcgattt
     1321 gaatgcaatg tgttctacca ctttatgatc caggaaaagg cgtatttcct cgattaaacc
     1381 atgatccgcg ccacactttt ctcataaaac tgctcattaa tcatcaggag ttgatcaaaa
     1441 caggggttcg gcgtattaaa gtttgctaac ctgtcgtacc aattaaaaca ggttgtatga
     1501 ctaatcagaa gggaacccat tgtgaaaaca ctaaatcgtc gcgattttcc cggtgcacag
     1561 tatccagaac gtatcattca gtttggtgaa ggtaacttcc tgcgcgcctt tgttgactgg
     1621 caaatcgatc tcctgaatga gcacaccgat ctgaattctg gcgtggtcgt tgttcgtccg
     1681 attgaaactt cattcccgcc gtcactgagc acgcaggatg gtctgtatac caccattatc
     1741 cgtggcctga atgagaaggg ggaagcggtc agcgacgcgc gtctgattcg ctcggttaat
     1801 cgtgaaatca gcgtctacag tgaatacgat gaattcctga aactggcgca caacccggaa
     1861 atgcgctttg ttttctctaa caccaccgaa gcggggatca gctaccatgc gggcgataaa
     1921 ttcgatgacg cgccagcgat aagctatccg gcaaaactga cccgtctgct gttcgaacgc
     1981 tttagccatt tcaacggtgc gctggataaa ggttggatca tcatcccgtg tgagttgatc
     2041 gactacaatg gcgacgcgtt gcgtgaactg gtgctgcgct atgcgcaaga gtgggcactg
     2101 ccagaagcat ttattcaatg gctggatcag gctaacagct tctgttctac gctggtggac
     2161 cgtatcgtta ccggttatcc gcgcgatgaa gtggctaaac tggaagaaga actgggttat
     2221 cacgatggtt tcctcgacac cgctgaacac ttttacctgt ttgtgattca ggggccgaaa
     2281 tccttagcga ctgagctgcg cctggacaaa tatccgctca acgtgctgat tgttgacgat
     2341 attaaaccgt ataaagaacg caaagtggca atcctcaacg gtgcgcacac cgcgctggtg
     2401 ccggtggcgt tccaggcagg gctggatacc gtaggtgaag cgatgaacga tgctgaaatt
     2461 tgcgcattcg ttgaaaaagc catctacgaa gaaattattc cggtactgga tttgcctcgt
     2521 gatgaactgg aatctttcgc cagtgcagtc accgggcgtt tccgtaaccc gtataagcat
     2581 cagctgctgt ctatcgcgct caacggtatg accaagttcc gcacccgcat cctgccgcag
     2641 ctgctggcag ggcagaaggc aaacggcaca cttccggcgc gccttacttt cgcattagcg
     2701 gcattgattg cgttctatcg tggtgagcgt aacggagaaa catatccggt acaggacgat
     2761 gcacactggc tggaacgtta tcagcaactc tggagccagc atcgtgacca tgttatcggt
     2821 acccaggaac tggtagcgat tgtgttggca gaaaaagacc actgggagca ggacctgacg
     2881 caagtgcctg gtctggtcga gcaggttgct aatgacctgg atgcgattct tgaaaaaggg
     2941 atgcgcgaag ctgtacgtcc gttgtgctaa taacaccgtt acttctgaac ccggttttga
     3001 ccgggtttct tatttcacta tattagttac aacatcctat gtgcctttat cattaacaga
     3061 tgccacatgc agcgcctgtt aatgttgtaa gcttgtaggt aatttgtaag tagcgtcttg
     3121 agaatgtgat cgtaatcacg tttaataggg cgactttttc acgcctgaag ataaccatga
     3181 ttgttcgtcc acaacaacac tggctgcgcc gtatttttgt ctggcacggc tcagtattat
     3241 ccaagatatc atcgcgctta ctactttgcg cataagcatt accagcgtcg cgcccattgc
     3301 cagcaggatc aggatttgcg cgctgctata aaccatggtc agcgtttgca cacttaaata
     3361 ctggcggtcg agaaaaccgg gtaatacaaa cagcagcacc accgccagca gtgccgtgat
     3421 ttcacggttg ttctgaataa acttcagcat gacgcctcct gacgctgact atcgccgaag
     3481 gcaacgcgca taatagtctc gacattaata tcgcgcccgg tcagtgcaga gtgggtaatt
     3541 tcgccctgat gcatcacata cacacgatct gccatcagtt cgatctcttc cagatcagag
     3601 gagataagca gcactgccac attctgtgcg gcgatgctgc gcaacagctg gtagatatca
     3661 ttacgggccg agacatccac gccgcgcgtc ggctcatcga caatcaatgc ttgcggcgaa
     3721 gcttccaggc atttggcaat caggattttt tgctggttgc caccggataa tgtccgtgca
     3781 gcttgttccg gttggttaaa tttaatattc agcgcccgac gataacgttc cagggtggca
     3841 ttatctttcg cggttttcgc ccagaatcca cgaaggttat gagtaagggc gcagacgttc
     3901 caggccagcg aagcatcgag attcagtccg gatg
//
LOCUS       DAJTYZ010000240         3915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000240 DAJTYZ010000000
VERSION     DAJTYZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.242"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..301
                     /locus_tag="OO596_004091"
     CDS             <1..301
                     /locus_tag="OO596_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719778.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..2258
                     /locus_tag="OO596_004092"
     CDS             390..2258
                     /locus_tag="OO596_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCR6719779.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2295..>3915
                     /locus_tag="OO596_004093"
     CDS             2295..>3915
                     /locus_tag="OO596_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6719780.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICP
                     YALMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTYADAFRESLPIIS
                     GELYFEAHQGCFTSESATKAHNRNMENKLHDA"
BASE COUNT         1230 a          721 c          766 g         1198 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgcaa agtaactttc tccaatatag agagttaata ttttcgagtg ttataattaa
     2281 ttaaagggat ttctatgtac cttactgata atattatcaa actgtcgaat aaaataggtc
     2341 agcgtgttta tactgttgtc tctgaaatgt cgattgaagc atggataaca aaagaaccgc
     2401 agccttttgt ttatcgccgt agcggcgctt accagaaact caccattggc tcagactggg
     2461 gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa acgtccagca gataaattta
     2521 gacacaaact cgtcgctctc gttgatatcg gtggcgaagg tcttattgtg gataagaatg
     2581 gttcacccat ctgcggcatc acgaataaag catcatctta tggcgtaccg ccagataaac
     2641 caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa tgacgaggtt gagttttgga
     2701 ttgatgctgc ctgcaacgat ttatttggct atgttactaa tggagggatt attactgatg
     2761 ttcatatcgc gacacgtaat caactactga aatcccttta ttatgatatt gaggtgcttt
     2821 tcgactggat taatgatggt cagaagtttg aatccataca tcctaaaggt atcaccccag
     2881 aacaatttat tgccaaaaga agtgagcgta ctgacgaaat cataagtact ctcgaatata
     2941 ttgataatac gttaattacc tttagcaatg aagaaataat taaatgtcaa attgcaatcc
     3001 aatccataat caataaaaac aataatcctt cagagttcag gattatggct actggtcatg
     3061 cccatttaga tatagcgtgg atgtggcctt taagagaagg acgcagaaaa gcaataagaa
     3121 catttgccac cgcccttgca aacattgaaa aatatcctga ttacattttt ggcgctagtc
     3181 agtaccaact ttttcactgg ataaaaaaag attatcccta tttcttcgaa aagttaaaaa
     3241 aacaaatcgc cgcaggccgc tttgagttac aaggttgctt ctgggtcgaa tgcgatttaa
     3301 atctggtaag tggtgaatcg ctgataaggc aaatcatgca tgggacacga ttcacactgc
     3361 agaatttttc aaaaaagata aattatgtct ggcaaccgga tgtctttgga ttccccgcaa
     3421 cgcttccaca aattctcaaa aaatcaggca ttaactatat tgcatcgcaa aagctctcac
     3481 aaaacaagat taataaattt aataattacc ttttccgctg gcagggcctg gatggctcag
     3541 aaattctgat gcataacttt cctgaagaca cttatgatag ccgagcacgt gctcgctctt
     3601 tagaatatat tgaacaaaac tataatgaaa aagagatctg tccctatgcg cttatggtgt
     3661 atggtgtcgg tgatggcggc gctggccccg gtgaggaaca tatagaacga ttaacacgta
     3721 tacgcaatat tgatggctta cctcatgttg atttcagccg tgttgataag ttcttcactt
     3781 acgccgacgc ctttcgcgaa agcttgccca ttatttcagg tgaactttat ttcgaggctc
     3841 atcaagggtg ctttacatca gagtcagcaa ctaaagctca taatcgaaat atggaaaata
     3901 aattacatga tgctg
//
LOCUS       DAJTYZ010000241         3831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000241 DAJTYZ010000000
VERSION     DAJTYZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.244"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..265)
                     /locus_tag="OO596_004094"
     CDS             complement(<1..265)
                     /locus_tag="OO596_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6719781.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            352..759
                     /gene="nudG"
                     /locus_tag="OO596_004095"
     CDS             352..759
                     /gene="nudG"
                     /locus_tag="OO596_004095"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6719782.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(725..997)
                     /locus_tag="OO596_004096"
     CDS             complement(725..997)
                     /locus_tag="OO596_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6719783.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1233..2576
                     /gene="gdhA"
                     /locus_tag="OO596_004097"
     CDS             1233..2576
                     /gene="gdhA"
                     /locus_tag="OO596_004097"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6719784.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2693..3732)
                     /locus_tag="OO596_004098"
                     /pseudo
     CDS             complement(2693..3732)
                     /locus_tag="OO596_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          997 a          932 c          917 g          985 t
ORIGIN      
        1 caagagaaat atcaagaaag ccgcccgcgt cggtaagctc tctgcgccgc gccagcgcac
       61 cgtccagacc atcaagcagc ctgttcaaca aaatgacgac tagcgccgcc agataccagc
      121 ccagcgccag aaacggcaga gccagcacgc cgatggcaaa tcccactaat gttaaaccat
      181 ctggcgtaat gcccggttta tcaagaaccc gcacgcactg atgcaacaac ggtttaatcc
      241 ggggatgaag atggcggtct agcacaggca ctccttaaat ataaagcctt tctgattgag
      301 caacagtgcg gatattatgg catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg
      361 attgaagttg ttgccgccat cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc
      421 gcccagagcg atcaggcggg attatgggag tttgccgatg gtaaagtcga gctggatgaa
      481 agccagcagc aggcactggt gcgtgagtta aacgaagaat tgggcatcga agcaactgtg
      541 ggtgagtatg ttgccagcca tcagcgagaa gtttcgggcc ggattatcca tcttcatgcc
      601 tggcacgtac ccgacttcca cgggacgtta caggcacatg aacatcaggc gctggtctgg
      661 tgctcacctg aagaggcgct gcaatatccg ctggcccctg ctgacattcc actattagag
      721 gcgtttatgg ctttacgcgc cgccagagca gcggattagt gctaagggtt ttgtcatccc
      781 gctggcattg cagcagtatt ccttcggctt taattaccgc cccttcagaa taattttgat
      841 cctgataaac gcagcactga gtacagggct gcgctgactg cccgcctgaa ctgaacactt
      901 ctggcggtac gtttacctcc acgtccggac gataatgcgg gttagccagt gcaattaatg
      961 gaaatgctaa tactacggcg aacaatgctc gactcacagg gaactcctta acgttattgt
     1021 ctctgcaact gataacggta gccgggtggc aaaactttag cgtctgagtt atcgcatttg
     1081 gttatgagat tactctcgtt attaatttgc tttcccgggt catttttttc ttgcttaccg
     1141 tcacattctt gatggtatag tcgaaaactg caaaagcaca tgacataaac aacataagca
     1201 caatcgtatt aatatataag ggttttatat ctatggatca gacatattct ctggagtcat
     1261 tcctcaacca tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg
     1321 aagtaatgac cacactctgg ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat
     1381 tactggagcg tctggttgaa ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg
     1441 atcgcaacca gatacaggtc aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc
     1501 cgtacaaagg cggtatgcgc ttccatccgt cagttaacct ttccattctc aaattcctcg
     1561 gctttgaaca aaccttcaaa aatgccctga ctaccctgcc gatgggcggt ggtaaaggcg
     1621 gcagcgattt cgatccgaaa ggaaaaagcg aaggtgaagt gatgcgtttt tgccaggcgc
     1681 tgatgaccga gctttatcgt catctgggcg cggataccga cgttccggca ggtgatatcg
     1741 gcgttggtgg tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata
     1801 ccgcctgcgt cttcaccggt aagggccttt catttggcgg tagtcttatt cgcccggaag
     1861 ctaccggcta cggtctggtt tatttcacag aagcaatgtt aaaacgccac ggtatgggtt
     1921 ttgaagggat gcgcgtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa
     1981 aagcgatgga atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg
     2041 atgaaagcgg attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg
     2101 atggtcgagt ggcagattac gccaaagaat ttggtctggt ctatctcgaa ggccaacagc
     2161 cgtggtctgt accggttgat atcgccctgc cttgcgccac ccagaacgaa ctggatgttg
     2221 acgccgcgca tcagcttatc gccaatggcg ttaaagccgt cgctgaaggg gcaaatatgc
     2281 cgaccaccat cgaagcgact gaactgttcc agcaggcagg cgtactgttt gcaccgggta
     2341 aagcggctaa tgctggcggt gtagcaacgt cgggcctgga aatggcacaa aacgctgcgc
     2401 gcctgggctg gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc
     2461 accatgcctg tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggcgcga
     2521 acattgccgg ttttgtgaag gttgccgatg cgatgctggc gcagggtgtg atttaagttg
     2581 taaatgcctg atggcgctac gcttatcagg cctacaaatg ggcacaattc attgcagtta
     2641 cgctctaatg taggccgggc aagcgcagcg cccccggcaa aatttcaggc gtttatgagt
     2701 atttaacgga tgatgctccc cagggaacat ttcttatggg ccaacggcat ttcttactgt
     2761 agtgctccca aaattgcttg tcgtaacgat aacacgcttc aagttcagca tccgttaact
     2821 ttctgcgata gcagcagata tgccagtaaa gaaaccccat tagactattt ttttgataat
     2881 cttcttcgct ttcgaacaac tcgtgcgcct ttcgagaagc aagcattata taatgccagg
     2941 ccagttcttc ttcaattgtc ccgttttgaa aagctgtgct tgatatcgag gtcatccatg
     3001 ataattccgc cgcccatatt agcttcgccg aggatttacc ggagcgatta ttagcgcaat
     3061 cagagatata gtctgaggga aaaacagcaa atttattcaa caaggcgata acctgctctg
     3121 gggcttcctc catgtttgct ttaaaggtat tggctccatg gtcgccagaa agaaaatgct
     3181 ccattaggca caataacttt cgctatcttc gataccccat tgattctcta aagactcgcg
     3241 tcttttactg atgatatcga tcgagtcaaa aggaagcaca tgatattgga aggtatcttt
     3301 accaggttca ggctttcgcg gccagaactc tagcgtttca gaccattgct tatgatagaa
     3361 tcgataaggt gcgatcaatt gtagcgcctg taacttctcg atactgagcg gctcaatacc
     3421 tttagcctga taataatgca gttgttcttt ttttgcttta aaaccggccc gaacaataag
     3481 ccccatcata attaatagat aaagaaaaga gcatcccgcg gtaatcaggc ctctttcatt
     3541 caaaccgttg gatgttatcg ctgcgaacac aaacattaca gcgacaacac atgttaaata
     3601 aaacccccac ttacaaagca gcatggcctt attttcttta atcatccgtt caaaattact
     3661 attaaatatt tcccagccat taaaagaata cttctcgctc ccaggatggt tttgtaataa
     3721 aacttttttc atcacattcc ctgttattac atactttaga ggcgataatt ttatccatgc
     3781 aaaaaaaata tccaacgaat tttcttgatc cgctggatat ttcagaatat g
//
LOCUS       DAJTYZ010000242         3805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000242 DAJTYZ010000000
VERSION     DAJTYZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.245"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2152
                     /locus_tag="OO596_004099"
     CDS             <1..2152
                     /locus_tag="OO596_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6719785.1"
                     /translation="AWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGME
                     RFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMA
                     GGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPY
                     VGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQ
                     TDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDT
                     LNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAA
                     ANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGD
                     TPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASELDC
                     WDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKI
                     LDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVS
                     ELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAE
                     EYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDA
                     PVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2386..3669)
                     /locus_tag="OO596_004100"
     CDS             complement(2386..3669)
                     /locus_tag="OO596_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6719786.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
BASE COUNT          915 a          952 c         1022 g          916 t
ORIGIN      
        1 tgcctggtct attctctctt ctcataatac gtccggaaat atggataaac ttaaaattaa
       61 gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga ccgctaaagc
      121 gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata actcactctg
      181 cgccgtcggc ggcaccatta acggtgatga ccatgttttt ggtttatcgg cagctcagcg
      241 ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata tgcgtgagat
      301 gatggcaggc gggggcaaaa tgatcctcgg gtcagacagc cacacccgtt acggtgcatt
      361 agggacaatg gcagtcggtg agggtggcgg tgagttggta aaacagctgc ttaatgacac
      421 ctgggatatc gactatccgg gcgtggttgc ggtgcatctg accggaaaac ctgcgccgta
      481 tgtggggcca caggatgtgg cgctggctat cattggcgcg gtgttcaaaa acggttacgt
      541 caaaaacaaa gtcatggagt tcgttggacc gggcgttagc gcgctctcta ccgatttccg
      601 taacagcgtt gacgtgatga ccacggaaac cacctgttta agctccgtct ggcaaactga
      661 tgaagaagtg cataactggc tggcgctgca cggtcgcggc caggattact gccagcttaa
      721 ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg ccatcaaacc
      781 aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac tgaaccagaa
      841 cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc acggtaaagc
      901 caaactctcg ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc aggggattat
      961 cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg cactgcgcgg
     1021 tcaatcctgt ggcaatgaca ccttctcgct ggcagtttac ccgtcatcac agccggtgtt
     1081 tatggatctc gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg caatcatcag
     1141 aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca acggtttgag
     1201 tattcgccac accacgcgta acttcccgaa ccgcgaaggc tctaagccag ctaatgggca
     1261 gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg caaacggtgg
     1321 ctatttaacc tctgccagcg aacttgattg ctgggacaac gtgccggagt acgccttcga
     1381 tgtaacgccg tataaaaacc gtgtttatca gggctttgtg aaaggggcaa ctcagcaacc
     1441 gctgatttac gggccgaaca ttaaagactg gccggaattg ggtgcactga ctgacaatat
     1501 cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg aactgattcc
     1561 ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt ttaccctgtc
     1621 tcgccgtgat cccggttatg ttggcagaag taaagcgact gctgagctgg aaaatcagcg
     1681 tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc agattgctgg
     1741 tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg cggtgaaacc
     1801 aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg gcggtctggc
     1861 gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca actgggggat
     1921 gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca tttacatccc
     1981 tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg tgatccatga
     2041 agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg aagagcgcga
     2101 gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt aaaaagcgcc
     2161 atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgcttgcg catcttatcc
     2221 gacctacgaa tcgcatcgaa tctgtaggcc agataaggca tttacgcagc atccggcact
     2281 tattgtcgga tgcgatgctt gcgcatctta tccgacctac aaatcgcatc gaaccgtagg
     2341 ccggataagg cgtttacgcc gcatccggca aatagttaat tgctcttact tcttcgcctc
     2401 tgcaaccact ttactaccca cgccgcggtt attgtattcc cacatgcggt tgtagttagt
     2461 gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat tacccgcaaa
     2521 cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac cttcgttgat
     2581 ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca gcgagcggcc
     2641 cagttgcgcc acaccatcac cgaaagcatt gaaacggctg tttacggcga ggaaaccgta
     2701 gtacatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag tacgtgagtt
     2761 caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa cgatatccac
     2821 atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg tttccagacg
     2881 gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa tgttaacgtt
     2941 gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt tacctgcatc
     3001 tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcaggc cattgttttg
     3061 tgaccagaag accgcagagc agagaacacc gatactgtcg ctgcgtttgc tctggcagct
     3121 atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt tgacgtcgtg
     3181 acgccagtcg gcagggctca tgccaccatc aagggaaagc ccaatcttca catcaatcgg
     3241 tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat ataccgttcc
     3301 ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct tgataattgc
     3361 cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtgcccg ccgggccgac
     3421 aacaaagtca ggttgcgcag gcagggtaat cggggaagga ttccacgctg cagcacctgg
     3481 tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg ccgacagaat
     3541 cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg gcggggttga
     3601 gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg aaactgaaaa
     3661 tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga agcctgcttt
     3721 tttatactaa gttggcatta taaaaaagca tcgcttatca atttgttgca acgaacaggt
     3781 cattatcagt caaaataaaa tcatt
//
LOCUS       DAJTYZ010000243         3799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000243 DAJTYZ010000000
VERSION     DAJTYZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.246"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(100..909)
                     /gene="proC"
                     /locus_tag="OO596_004101"
     CDS             complement(100..909)
                     /gene="proC"
                     /locus_tag="OO596_004101"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6719787.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1029..1487
                     /locus_tag="OO596_004102"
     CDS             1029..1487
                     /locus_tag="OO596_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6719788.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1670..2194
                     /gene="aroL"
                     /locus_tag="OO596_004103"
     CDS             1670..2194
                     /gene="aroL"
                     /locus_tag="OO596_004103"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6719789.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2244..2435
                     /gene="yaiA"
                     /locus_tag="OO596_004104"
     CDS             2244..2435
                     /gene="yaiA"
                     /locus_tag="OO596_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6719790.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2693..3370
                     /locus_tag="OO596_004105"
     CDS             2693..3370
                     /locus_tag="OO596_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6719791.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3442..3726
                     /gene="ppnP"
                     /locus_tag="OO596_004106"
     CDS             3442..3726
                     /gene="ppnP"
                     /locus_tag="OO596_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6719792.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          952 a          904 c          990 g          953 t
ORIGIN      
        1 atcgtgagtg gttgaaatcg gcagatttgg cgctttacaa agcaaagaaa gccggacgta
       61 accgcaccga agtggcggcc tgacgtccgg cgaaagtcat caggatttgc tgagtttttc
      121 tgatttttcc atacacttcg tcatcgcttc gatcactgcg gcacggaagc ctttctcttc
      181 cagtacgcgt accgcttcaa tggtggtgcc tcccggtgag cagaccatat ctttcagtgc
      241 ccccggatgt tctcccgttt ccagcaccat ttttgcggaa cccattaccg cctgagcggc
      301 aaatttatac gcctgggcgc gtggcatccc gcccagcacg gcggcgtcgg ccatcgcttc
      361 gataaacata aatacgtagg ctggcgaaga accgctcaca ccgaccaccg ggtggatcat
      421 cggctcagca attacttccg cttcgccaaa gcagcggaaa atattcagca catcagcggt
      481 atcttcgggg gttaccagcg cgtttggcgt tacggaggtc atcccggcat taaccagcgc
      541 gggagtgttc ggcatggcgc ggataatttt ccggtcatgg cccagcgcgc gggcaagctg
      601 gtcgagcgtg acacctgcag caatagaaac gaccagagag tctttattca ggctggaggt
      661 gatttcgcta agcactttaa tcatgatgcc aggtttaacg gcggcaaaaa tgatgtcggc
      721 gatttgcgcc acttcttgcg ccgattctgc ggcgttgatg ccgaactggt catgcagggc
      781 ggcgacttta tccggggagg gggtgtatac ccagatttgc cctggaagca cctgaccgct
      841 ggcaatcaga ccgccgagaa tggcttttcc catattgccg cagccaataa aaccgatttt
      901 cttttccatt gcctcactcc tgccgtgaaa ttcattgttt tgataatcgc tggcagaagc
      961 ataaacagaa ctatgccgga aggcaaaagc gcgacacaat agaggattac ccaacaaagg
     1021 atgactttat gacaatttgg gtggatgccg acgcgtgtcc caatgtaatt aaagagattt
     1081 tgtatcgcgc ggcggaacgt atgcagatgc cgctggtact ggtagcaaac cagagtttac
     1141 gcgtgccgcc atcgcgattt attcgtacgc tgcgcgtcgc ggcaggtttc gacgttgccg
     1201 ataacgaaat tgtccggcag tgtgaagcgg gcgatttggt gatcaccgca gatatacctt
     1261 tggctgctga agccatcgag aaaggcgcag cggcgcttaa tccgcgcggc gaacgttaca
     1321 cgccagcgac cattcgtgag cgcctgacga tgcgcgattt tatggatacc ttacgtgcca
     1381 gtgggatcca gaccggcgga ccagatagcc tttcacaacg tgaccgccag gcctttgccg
     1441 cggagctgga gaagtggtgg ctggaagtgc aacgtagtcg tggctaaatg taatttatta
     1501 tttacacttc attcttgaat atttattggt atagtaaggg atgtattgag attttcactt
     1561 taagtggaat tttttcttta caatcgaaat tgtactagtt tgatggtatg atcgctattc
     1621 tcatgacacc ggctttcgcc gcattgcgac ctattgggga aaacccacga tgacacaacc
     1681 tctttttctg atcgggcctc ggggctgtgg taaaacaacg gtcggaatgg cccttgccga
     1741 ttcgcttaac cgtcggtttg tcgataccga tcagtggttg caatcacagc tcaatatgac
     1801 ggtcgcggag atcgtcgaaa gggaagagtg ggcgggattt cgcgccagag aaacggcggc
     1861 gctggaagcg gtaactgcgc catccaccgt tatcgctaca ggcggcggca ttattctgac
     1921 ggaatttaat cgtcacttca tgcaaaataa cgggatcgtg gtttatttgt gtgcgccagt
     1981 atcagtcctg gttaaccgac tgcaagctgc accggaagaa gatttacggc caaccttaac
     2041 gggaaaaccg ctgagcgaag aagttcagga agtgctggaa gaacgcgatg cgctatatcg
     2101 cgaagttgcg catattatca tcgacgcaac aaacgaaccc agccaggtga tttctgaaat
     2161 tcgtagcgcc ctggcacaga cgatcaattg ttgattttag agcgcctata cttaacgttc
     2221 atcccgtgaa ataaggaaga acgatgccaa cgaaaccgcc ttatcctcgt gaagcatata
     2281 tagtgacgat tgaaaaagga aagccaggac agacggtaac ctggtaccaa ctcagagccg
     2341 atcatcctaa accagactcg ttgatcagtg aacatccgac ggctcaggaa gcgatggatg
     2401 cgaaaaaacg ctatgaggac cctgacaaag agtgaccgca tcagactgct cggaagggat
     2461 tctgagtgcc actacaaggg atctgcgtca catttttcat aattcatgtt tttctaataa
     2521 ttagaatatt aaacaataac aatccattac tggaatcatt tggaatcttt acattatgcc
     2581 gtgcacgtct gctgctacgc tttttgtcat ttgtagcaca agtaagtgtc agcagtggtg
     2641 ctccacactt gcccggtaat taacgacgaa agaaaagtaa ggtggatgaa caatgagtgc
     2701 gtcgttggcg atcctgacca tcggcattgt acctatgcag gaggttttgc cgctcctgac
     2761 ggaatacatt gacgaagata atatttccca tcatagcctg ctggggaagt taagtcgtga
     2821 agaagtgatg gcggagtacg cgccagaagc aggcgaagac accattctca cattattaaa
     2881 tgacaaccag ctggcccatg tttcgcgtcg taaagtggag cgagacctgc aaggtgtggt
     2941 tgaagtgctc gataatcagg gttatgacgt cattttatta atgagtacag caaacattag
     3001 tagtatgact gcgcgtaata cgatctttct tgagccgtcg cgaatattgc ctccactggt
     3061 ttcctctatt gttgaagatc atcaggtggg ggttatcgtt ccggttgagg agatgctgac
     3121 cgttcaggcg caaaaatggc aaattttgca gaaatcgccg gtattttcat tgggtaaccc
     3181 cattcatgat tcagaacaaa aaatcattga tgccgggaaa gaattactgg caaaaggggc
     3241 tgatgtcatc atgctggatt gtttaggatt tcaccaacgt catcgcgatt tactgcaaaa
     3301 acagctcgat gttcctgtct tgctgtctaa cgtattgatt gcacggctgg ctgcggaatt
     3361 actggtgtaa ttttgcgtga cagccagcgc ctctggcccc tatagtgaag tagatgttca
     3421 actaccagac agggccagtt tatgcttcaa agtaatgagt acttttccgg caaagtgaaa
     3481 tcaatcggct tttccagcag cagcactggt cgcgccagcg tgggtgttat ggttgaaggc
     3541 gaatacacct tcagcaccgc tgagccggaa gagatgacgg taatcagtgg cgcgctgaat
     3601 gtgttactgc ctgacgcgac cgactggcag gtgtatgaag ccggttcggt gtttaatgtt
     3661 cccggtcaca gtgagtttca tctgcaagtt gccgaaccca cctcttatct gtgccgctat
     3721 ctgtaattcc tcgccttccc cttgaacggg agggcatttt tctgaaatat cctctcttta
     3781 acccataata atatttcct
//
LOCUS       DAJTYZ010000244         3701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000244 DAJTYZ010000000
VERSION     DAJTYZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.247"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            105..518
                     /locus_tag="OO596_004107"
     CDS             105..518
                     /locus_tag="OO596_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6719793.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(563..880)
                     /gene="hspQ"
                     /locus_tag="OO596_004108"
     CDS             complement(563..880)
                     /gene="hspQ"
                     /locus_tag="OO596_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6719794.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(938..2128)
                     /gene="rlmI"
                     /locus_tag="OO596_004109"
                     /pseudo
     CDS             complement(938..2128)
                     /gene="rlmI"
                     /locus_tag="OO596_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2223..2501
                     /gene="yccX"
                     /locus_tag="OO596_004110"
     CDS             2223..2501
                     /gene="yccX"
                     /locus_tag="OO596_004110"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6719795.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2498..2827)
                     /gene="tusE"
                     /locus_tag="OO596_004111"
     CDS             complement(2498..2827)
                     /gene="tusE"
                     /locus_tag="OO596_004111"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6719796.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2918..3577)
                     /gene="yccA"
                     /locus_tag="OO596_004112"
     CDS             complement(2918..3577)
                     /gene="yccA"
                     /locus_tag="OO596_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6719797.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
BASE COUNT          971 a          942 c          936 g          852 t
ORIGIN      
        1 acatatagac atatttcccg cttttgctct cattcattcg tattagctgc atggttggca
       61 tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa gaaaccgata
      121 ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc gacaaacccg
      181 atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac gtcattccgg
      241 tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc acgctggcag
      301 acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg gcgtggggcg
      361 tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg ggggtgatta
      421 atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg gatcgctgcc
      481 cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga aggcaaaaac
      541 cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt tgtttgcgga
      601 tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc aattcgctac
      661 ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc tccatcacta
      721 cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca ggcgacggtt
      781 cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct aacagggaat
      841 ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc cgggaaatct
      901 ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga caggcaaacc
      961 ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct gcctgacgga
     1021 actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg atgattttct
     1081 gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata ccgccttcat
     1141 tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg cccatcaact
     1201 ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat ttttcaccgc
     1261 gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca gccttgctca
     1321 gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc taggaggtat
     1381 caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa ccaccggtat
     1441 aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc aggcggctgt
     1501 ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc agcagcttca
     1561 tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg aggccctggg
     1621 tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa atcgcacatt
     1681 ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct gccccagcac
     1741 tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc ggcaggccat
     1801 cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc agtcagtcac
     1861 gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata gactcagacg
     1921 ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg ccgcgtgcta
     1981 accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct ttaccttcca
     2041 tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat ttttcgcgcc
     2101 ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca aagaaatggg
     2161 cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata aggagagcag
     2221 caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc gtaggatttc
     2281 gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc aaaaatcttg
     2341 acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa aaattaatgc
     2401 agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc agcgagccgc
     2461 atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt accggttttg
     2521 gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt cggtataaat
     2581 agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc agcatacgaa
     2641 tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc acttcccagt
     2701 gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc ggctcactcc
     2761 actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta ccttcgaaga
     2821 tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa acaaagcccc
     2881 gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg gcgaagccca
     2941 gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta accgtggcac
     3001 gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa atagcgccag
     3061 aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc tgcaggaaga
     3121 tattagcaac cataccaatc agcaccacca caatacccgc catcagcata ccgccgagga
     3181 acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac actaacgccg
     3241 ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag gtgttcagaa
     3301 taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata atcccggtcg
     3361 gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc agcgtcagaa
     3421 tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc gccgaaaagg
     3481 ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg ctaagcagtg
     3541 atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct atgacagatg
     3601 taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt acccatcttt
     3661 acgcatttga tctggaacag gtttaacagc ggattatcag g
//
LOCUS       DAJTYZ010000245         3693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000245 DAJTYZ010000000
VERSION     DAJTYZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.248"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..110)
                     /locus_tag="OO596_004113"
                     /pseudo
     CDS             complement(1..110)
                     /locus_tag="OO596_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
     gene            complement(344..2053)
                     /locus_tag="OO596_004114"
     CDS             complement(344..2053)
                     /locus_tag="OO596_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6719798.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2485..3204)
                     /locus_tag="OO596_004115"
     CDS             complement(2485..3204)
                     /locus_tag="OO596_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6719799.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPNYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3329..>3693)
                     /locus_tag="OO596_004116"
     CDS             complement(3329..>3693)
                     /locus_tag="OO596_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6719800.1"
                     /translation="QEKLIWLRAQMKLTSLCWALKELAKDIWSRPWSEERRNDWQRWL
                     SLAANSDVPMMKNVAKTIGKRLYGILNAMRHGVSNGNAEALNSKIRLLRIKAKGYRNR
                     ERFKLGVMFHYGKLNMAF"
BASE COUNT         1089 a          757 c          626 g         1221 t
ORIGIN      
        1 tttttgttat aacaaaactc tcggaatcct tgatctttac tctttcttat aaaatcaata
       61 agatgcaacc caattccaag cctcatatct ttagtattaa atattggaac atactcaccc
      121 tctggatgtg acctttatcc atgaagaaga tttggccaca ccatggaaac ctgaacaatc
      181 atcagtttac gcccatgtaa acccctacgg gatatttgaa ctcgatatgg aaataaggtt
      241 gctaatcgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
      301 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      361 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      421 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      481 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      541 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      601 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      661 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      721 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      781 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      841 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      901 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      961 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
     1021 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
     1081 tgcacatatt taatatctta ccatatactt gaataatgtc tgaacatccg ttttgaagtg
     1141 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
     1201 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1261 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1321 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1381 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1441 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1501 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1561 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1621 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1681 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1741 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1801 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1861 tatttacctt cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1921 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1981 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     2041 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     2101 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     2161 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     2221 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2281 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaagtggt ggacaactgt
     2341 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2401 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2461 aaggtggtgt agctggcatc ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2521 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2581 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2641 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2701 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2761 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2821 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2881 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2941 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     3001 agaaaaaaat tgacatatat cagagtaatt tggcacgttc gatgctactt tgaaatagta
     3061 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     3121 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     3181 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3241 tattgattgt ctatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3301 gggtcttctc cgatcatggt gggaaggctt agaacgccat attcagtttc ccatagtgga
     3361 acatcactcc cagcttaaag cgctcccggt tgcggtagcc cttggctttt attctcagca
     3421 gcctgatctt gctgttcagt gcttccgcat ttccgtttga tacgccatgg cgcatggcgt
     3481 tcaggatacc gtacaacctt ttccctatcg ttttagccac attcttcatc atggggacgt
     3541 cactgttggc cgccagcgac agccatctct gccagtcgtt gcgtctttcc tcgctccatg
     3601 gtctgctcca gatatccttt gccagttctt tcagcgccca gcacaggctc gtcagcttca
     3661 tctgcgcacg cagccatatc agcttttcct gcc
//
LOCUS       DAJTYZ010000246         3575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000246 DAJTYZ010000000
VERSION     DAJTYZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.249"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            25..519
                     /locus_tag="OO596_004117"
     CDS             25..519
                     /locus_tag="OO596_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6719801.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            516..2249
                     /locus_tag="OO596_004118"
     CDS             516..2249
                     /locus_tag="OO596_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6719802.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            2261..2443
                     /locus_tag="OO596_004119"
     CDS             2261..2443
                     /locus_tag="OO596_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719803.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            2443..3575
                     /locus_tag="OO596_004120"
                     /pseudo
     CDS             2443..3575
                     /locus_tag="OO596_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          830 a          895 c         1074 g          776 t
ORIGIN      
        1 gaaatttaac caggagtgcc gcatatggct ggaacggcgg ggcgttccgg gcgtcgcccc
       61 aagccaacgg cgcgcaaggc gctggccgga aaccccggca agcgagccct gaacaaagat
      121 gaacctgttt ttacgcccat caaaggtgtt gagccaccag agtggttcgc tgaagaagat
      181 ctccctctcg ccacgatcat gtggcaactg acaaccaaag aactctgcgg tcagggcctg
      241 ctgtgcgtga ctgacctggc ggtacttgag cggtggtgcg tggcctatga gttctggcga
      301 cgtgccgtga aaaatattgc catacagggc aacaccatca ccggtgcaat gggcggcagg
      361 gtcaaaaatc cggagctgac cgccaaaaaa gaacaggagt ccgagatgag cagcacgggg
      421 gcaatgctcg gactcgaccc cagcagccgc cagcgtctga ttggcctggc ggggcagaag
      481 aaagccacta acccgtttct gaaaattatc gaatcatgag ccggaaatct taccccaacg
      541 taaatgctgc aaatcagtat gcccgggatg tcgtgcgcgg aaagattgtt gcctgccagt
      601 ttgtgattca ggcctgccag cgccatcttg atgacctgat ggcggaaaaa agtaagtcgt
      661 ttcgttaccg cttcgacaag gacctggctg aacgggccgc gaaatttatt cagctgttgc
      721 cgcacaccaa gggggagtgg gcattcaaac ggatgcccat cacgctggag ccgtggcagc
      781 tatttgtgat ctgctgtgcg tttggctggg tcaataaagg cacccggttg cgccgcttcc
      841 gggaggtgta caccgaaatc ccccgtaaga acggcaaatc agcaatctct gccggtgttg
      901 ccctgtattg ttttgcctgt gataacgagt ttggcgcgga agtgtattcc ggtgccacga
      961 cagagaaaca ggcgtgggaa gtctttcgcc cggcgcgact gatgtgtaaa cgcacaccca
     1021 tgctgacgga agcgttcggg attgaggtta acgcctcaaa catgaaccgt ccggaggatg
     1081 gcgcgcggtt tgaaccgctg atcggcaacc caggtgatgg ttcatcaccc cactgtgccg
     1141 tggttgatga atatcacgag catgccaccg atgcgcttta taccacaatg cttaccggga
     1201 tgggggcgcg acgtcagcca ctgatgtggg ccatcaccac cgccgggtac aacattgagg
     1261 ggccgtgcta cgacaagcgg cgggaagtca tcgagatgct caacggctcg gtgcctaacg
     1321 atgaactgtt cgggatcatc tataccgttg atgaaggtga cgactggacc ggcccgcagg
     1381 tgctggaaaa agccaatcca aatattggcg tgtcggttta tcgcgaattt ttgttaagtc
     1441 agcagcagcg tgcgaaaaat aacgcccgtc tggcaaacgt ctttaaaaca aaacacctca
     1501 atatctgggt gtcggcgcgt tcggcgtatt tcaacttggt gagctggcag agctgcgagg
     1561 ataaatcact gacccttgag cagttcgagg ggcagccgtg cattctggcc tttgacctgg
     1621 cgcgtaagct ggatatgaac agcatggcgc gactttatac ccgcgagatt gacggtaaaa
     1681 cgcattacta cagtgtggcc ccgcgtttct gggtaccgta tgacacggtg tacagcgtcg
     1741 agaaaaatga agatcgacgg acagccgaac gctttcagaa atgggtggaa atgggcgttc
     1801 tgaccgttac cgatggtgcg gaggtggatt atcgctacat cctcgaggag gccaaagcgg
     1861 cgaacaaaat cagcccggtc agtgagtcac ccatcgaccc cttcggggcg accgggttgt
     1921 cacatgacct tgctgatgaa gacctgaatc ccgtcactat cgtccagaac ttcaccaata
     1981 tgtccgatcc gatgaaagag ctggaagcag cgattgaatc gggacgtttt catcatgacg
     2041 gcaatcccat catgacctgg tgtatcggca acgtggtcgg caaaaacatg ccgggtaacg
     2101 atgatgtggt gaaacccgtc aaagagcagg cggaaaacaa aatcgatggt gcagttgcgc
     2161 tgattatggc ggttggcaga gccatgctgt acgagaaaga agacacgctg tctgaccaca
     2221 ttgagtccta cgggatccgc tcgctttaac tgaggtaatt atgatcatgc tgattctcgc
     2281 gcctctggtg ggcgtgctgg gtgcgctttt gctggcgtat ggtgcctggc tgatttatcc
     2341 cccggcgggt tttgttgttg ccggggcgct gtgcctgttc tggtcgtggc tggtggcgcg
     2401 atatctcgac cgtacacggc agtctgtcgg cggaggtaaa tagtgttctt ttcgggatta
     2461 tttcaacgaa aaagcgatgt gccggtgacc acgccagcag aactgctgat gctatcgggc
     2521 tgtcatacga cacctatacc ggaaagcgga tcagtagcca gcgggccatg cgcctgacgg
     2581 cggtctattc ttgcgtcagg gtgctggcag agtctgttgg tatgctgccc tgcagtctct
     2641 acaaaatcag cggtaccctg aaaacacggg cggtggatga acgactgcat aagttggttt
     2701 cggcaaaacc taatggctac atgacaccgc aggaattctg ggagctggtt attgtctgcc
     2761 tgtgtctacg ggggaatttt tacgcctaca aggtaaaggc actgggagaa gtggtggagc
     2821 ttcttccgat agatccgggc tgtgtggaac cgaagctgaa cagccagtgg cagccggttt
     2881 atcaggtgac gtttccggat ggttccgtgg atgtgttgac ccagaatgaa atctggcatg
     2941 tgcgcaccct gacgctggat ggacttgtcg ggctgaatcc cattgcgtat gcgcgtgagg
     3001 ccatttcact ggcagcggca accgaggagc acggtgccag gttgtttagt aatggtgcgg
     3061 tgacatccgg tgtgttgcgt acggaacaaa agctcacgcc ggatgcttac gagcgtctga
     3121 agaaagattt tgaggagtgt cacacggggc ttggcaatgc tcaccgcccg atgattctgg
     3181 aaatggggct ggactggaag tcgatggcgc tgaacgccga ggacagtcag ttcctggaaa
     3241 cccgcaagtt tcagctggaa gaaatctgtc gtctgttccg ggtgccgttg cacatggtgc
     3301 agaacaccga tcgcgccacc ttcaacaata tcgaagagct ggggctggga tttatcaact
     3361 attcactggt gccgtatctg acccgcatcg aacagcggat caacaccgga ctggtacgaa
     3421 aaagtaagca gggcgtttat tacgccaaat ttaacgccgg ggcgttactg cgcggggata
     3481 tgaagtcccg ttttgaagcc tacgccaccg ggatcaactg gggaatttac tcttccaatg
     3541 actgccgcga cctggaagat atgaatccgc gtccc
//
LOCUS       DAJTYZ010000247         3527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000247 DAJTYZ010000000
VERSION     DAJTYZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.250"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..351
                     /locus_tag="OO596_004121"
     CDS             <1..351
                     /locus_tag="OO596_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6719804.1"
                     /translation="RWYLCPQVRVADIVQLNGNIRPRSRQWWQLFRMVSQWHVDVVIV
                     ELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWL
                     NTTGADQQSPEHRS"
     gene            591..851
                     /gene="repA"
                     /locus_tag="OO596_004122"
     CDS             591..851
                     /gene="repA"
                     /locus_tag="OO596_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6719805.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1075..1149
                     /gene="tap"
                     /locus_tag="OO596_004123"
     CDS             1075..1149
                     /gene="tap"
                     /locus_tag="OO596_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6719806.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1142..1999
                     /gene="repA"
                     /locus_tag="OO596_004124"
     CDS             1142..1999
                     /gene="repA"
                     /locus_tag="OO596_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6719807.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESDAGTLSITRATRALTFLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDV
                     SEEAVASARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2699..2890
                     /locus_tag="OO596_004125"
     CDS             2699..2890
                     /locus_tag="OO596_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719808.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTAACRLT
                     CRWRGIRIIQAAFNAFAKV"
     gene            2963..3214
                     /locus_tag="OO596_004126"
     CDS             2963..3214
                     /locus_tag="OO596_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR6719809.1"
                     /translation="MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            3211..3498
                     /locus_tag="OO596_004127"
     CDS             3211..3498
                     /locus_tag="OO596_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6719810.1"
                     /translation="MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
BASE COUNT          934 a          867 c          903 g          823 t
ORIGIN      
        1 cggtggtatc tgtgtccgca ggtacgggtc gcggatatcg tccagctgaa cgggaatatc
       61 cggccacgat cacgccagtg gtggcagtta ttcaggatgg tgtctcagtg gcatgttgat
      121 gtggttatcg ttgagctgcg ttcgttcagt attgttgcag cagtagagct ggatgatgcc
      181 agccatttac gaccggaacg cagacgccgg gatattcttc tggaagaggt tctgaggcag
      241 gctggtattc cgttgctcag aagccacgat gccagaaaac tgctgcagat gaccggagaa
      301 tggctgaata caacaggggc tgatcagcag tcaccggaac atcgtagctg acgccttcgc
      361 gttgcttagt tgtccaaccc cggaaacggg aaaaagcaag ttttcctcgc tcccggcgtt
      421 tccataactg aaaaccatac tatttcacag tttaaatcac attaaacgac agtaatcccc
      481 gttgatttgt gcgccaacac agatcttcgc cacaattctc aagtcgctga tttcaaaaaa
      541 ctgtagtatc ctctgcgaaa cgatccctgt ttgagtattg aggaggcgag atgtcgcaga
      601 cagaaaatgc agtgacttcc tcattgagtc aaaagcggtt tgtgcgcaga ggtaagccta
      661 tgactgactc tgagaaacaa atggccgcag ttgcaagaaa acgtcttaca cacaaagaga
      721 taaaagtttt tgtcaaaaat cctctgaaag atctcatggt tgagtactgc gagagagagg
      781 ggataacaca ggctcagttc gttgagaaaa tcatcaaaga tgaactgcag agactggata
      841 tactaaagta aagactttac tttgtggcgt agcatgctag attactgatc gtttaaggaa
      901 ttttatggct ggccacgcca taaggtggca gggaactggt tctgatgtgg atgtacagga
      961 gccagaaaag caaaaacccc gataatcttc tttaactttg gcgagtcaga aagattaccg
     1021 gggcccactt aaaccgtata gccaacaatc aagctatgcg gggagtatag ttatatgccc
     1081 ggaaaagttc aagacttctt tctgtgctcg ctccttctgc gcattgtaag tgcaggatgg
     1141 tgtgactgat cttcaacaaa cttattaccg ccaggtaaag aacccgaatc cggtgttcac
     1201 accccgtgaa ggtgcccgga cgcttccgtt ctgcgggaaa ctgatggaaa aggcggtggg
     1261 cttcacctcc cgttttgatt tcgccattca tgtggcacat gcccgttcgc ttggtctgcg
     1321 tcggcgcatg ccaccggtac tacgtcgccg ggccattgat gcgctgctgc aggggctgtg
     1381 tttccactat gacccgctgg ccaaccgcgt tcagtgctcc atcaccacgc tggccattga
     1441 gtgcggactg gcgacggagt ctgatgccgg aacactttcc atcacccgtg ccacgcgtgc
     1501 cctgacgttc ctggcagagc tggggctgat tacctaccag acggaatatg acccgcttat
     1561 cggctgcaac attccgaccg acatcacgtt cacaccggcg ctgtttgccg ccctcgatgt
     1621 atcagaggag gcggtggcgt ccgcgcgccg cagtcgtgtg gaatgggaaa acagacagcg
     1681 caagaagcag ggactggata ccctgggtat ggatgaactg atggcgaaag cctggcgttt
     1741 tgtgcgtgag cgtttccgca gttaccagac agagcttaag tcccgtggaa tgaagcgtgc
     1801 ccgtgcgcgt cgtgatgcgg acaggcagcg tcaggatatt gtcaccctgg tgaaacggca
     1861 actgacgcgt gaaatctcgg aagggcgctt cactgccagt cgtgaggcgg taaaacgtga
     1921 agtggagcgt cgtgtgaagg agcgcatgat tctgtcacgt aaccgtaatt acagccggct
     1981 ggccacagct tccccctgaa agtgacctcc tcagaataat ccggcacgcg ccggaggcat
     2041 tcgcacgcct gaagcccgcc agtgcacaaa aaatcagcgc tacatacaaa aaacaacctc
     2101 atcatccacc ttctggtgca tccggtcccc cctgtttttg atacaaaaca cgcctcacag
     2161 acggggaatt ttgcttatcc acattaaact gcaagggact tccccataag gttacaaccg
     2221 ttcatgtcat aaagcgccag ccgttagctt tacagggtgc aatgtatctt ttaaacagct
     2281 gtttatatct cctttaaact acttaattac atccatttaa aaagaaaacc tgttcactgc
     2341 ctgtcctgtg gacagacaga tatgcacctc ccaccgcaag cggcgggccc cgaccgaagc
     2401 cgatttagtt acaacatgca aacataaacc cctaaaaaac ccaagtccga cgcagaactg
     2461 aaaccacaac gcccctcctc ataactgaaa agcgccgctg cccccgcccg aagggcggga
     2521 acagagtcgc ttttaattat gaatgttgta actacatcgt catcgctgtc agtcttctgg
     2581 ctggacgtat cgagtacacg ctcgtaagcg gccctgacgg cccgctaacg cggagatacg
     2641 ccccgacttc gggtaaaccc tcgtcgggac cactccgacc gcgcacagaa gctctgtcat
     2701 ggctgaaaac gggtatggct tagcagggct ggggatgggt aaggtgaaat ctgtcaatca
     2761 gtaccggcta acgccgggct tcggcggttt tactccggta tcacatgtaa ccgcagcgtg
     2821 ccgccttaca tgccgctggc gcggcatcag aataatacaa gcagcattta atgcatttgc
     2881 aaaagtgtga gtaataaaga cttgttaagt ggtcgtttca gggatatcgt taagacttgt
     2941 taaacagtct caaaggtgcg ttatgcctaa catcattctg tctgagacca gtgcaagtgt
     3001 cagtgaactg aaaaaaaatc ctatggctac agtcagtgcc ggagatggtt accctgtcgc
     3061 cattcttaac cggaatcagc cggcattcta ctgtgtgcct gctgaactgt atgaaaggat
     3121 gcttgatgct ctggatgacc aggaactggt gaaactggtt actgaacgca gcaatcagcc
     3181 actgcatgat gtggacctgg acagttattt atgacttata cggtaaaatt cagggatgat
     3241 gcgctcaagg aatggctgaa actggataag accattcaac aacagtttgt gaaaaaacta
     3301 aaaaaatgca gcgaaaatcc tcatataccg tcggcaaaac taagagggct aaaggactgc
     3361 tacaaaatta aactacgtgc atcgggattt cgtctggttt atcaggtgat tgacgatatg
     3421 ttaattattg cggttgttgc ggtaggaaaa cgagaacgta gtaacgttta taatctcgcc
     3481 agtgagagaa tgagatagta acatataaat aacctttccc gcagata
//
LOCUS       DAJTYZ010000248         3491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000248 DAJTYZ010000000
VERSION     DAJTYZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.251"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(75..1472)
                     /locus_tag="OO596_004128"
     CDS             complement(75..1472)
                     /locus_tag="OO596_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6719811.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            1627..2625
                     /locus_tag="OO596_004129"
     CDS             1627..2625
                     /locus_tag="OO596_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6719812.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(2736..>3491)
                     /gene="ompG"
                     /locus_tag="OO596_004130"
     CDS             complement(2736..>3491)
                     /gene="ompG"
                     /locus_tag="OO596_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR6719813.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT          874 a          913 c          860 g          844 t
ORIGIN      
        1 ctgcgccctg aattttggat tctgatcgac ctccagcgga ccgtcgaaat caatacgtgg
       61 ttttaacggt tcggtcatcg caatttatct cctattaaaa attccagcgc ggcatcaaga
      121 cgaatatgcg gcagcggttt gtcgacatcc atcacctgcg ggcgaaacgc ttcaaactgg
      181 aacccttgct tgtcccagaa cgcctgaccg ggcaaacgcg caggaacttc accggggtaa
      241 acggtgagag gcgcaccatc gctgagccga ttaccgcgca atgccggaat tttctcgccg
      301 ttgacatcaa taatgccgct ggtggtcgcc tgcaccgagg ccagccccag gcaatccata
      361 ctaatccctt caaacgccgc attttgccag gcatcctgaa tcagttgttg cagtaatgaa
      421 accatattgg cgtgctgatc gaccgttaca tggtccgctt tcgttgcggc aaacagcagt
      481 ttgtcgataa ccggcgagaa taagcggcga aagagcgtgc gctgcccgta gtggaaactt
      541 tgcatcagct gcgtcagtgc cagacgcata tcattaaatg cctgtggccc actgttgaga
      601 ggttgcaggc aatccaccag cacaatctgg cggtcaaagc gcagaaaatg attcttatag
      661 aaccccttca ccaccttctc gcagtaataa ttaaaccgct cgcgcagcat tccggcattg
      721 gtgtgcttat cggcctgcgc cagtttggac tcgccccagg tatcgacatc cggccacggg
      781 aagaattgca gcgcgggcgc acctgccata tctcctggca agacaaagcg ccctggctga
      841 ataaagtgca gcccctgctg tttacagtgg tggagataat cggtccacgc ggcggcaatg
      901 tctgccagcc ggttttcgtc ggcaggtgct agcgggtcca gcccttcgca catcattcgc
      961 catttgaccg accattcgcc gcgctgacca ttgagtaagc ccgtcatctg gcgtgaccag
     1021 cttaaatagt cctgcgccag catcggcagg tcgagcaacc attcgccggg ataatccaca
     1081 atctccagat acagcgtgga ggtgtctttg aagtggcgta gcagcgaatc gttcgattta
     1141 aaacgtagcg ccaggcggat ttcactgacg ccgcgcgtag gcgtcggcca ggcgggagga
     1201 tcgccatata actgcgccag tccttcatca taggtgaagc gcggaatgcc aaagtcacgc
     1261 tgaggaatgc gttttacgcc cagcaggcgc tcttcacgca ccgcgcttaa cagcggcaag
     1321 cgtgctccgg cgtgaatatt gagcaactga ttgaccatcg cagtgataaa cgctgttttg
     1381 ccgctgcggc ttagtccggt tacggcgagg cgcagatgtc tgtcgacacc ccgattcacc
     1441 agcgcattaa gttcattttt aagtcgcttc attgctgtcc tgtgctgctc tgaagaccag
     1501 aaaaaaatca ttcgtaagga tatgaatgtg ggggccaggc cattgattat caactgcccg
     1561 tgttgtcatc gacgggcgaa ctccataaga tgtgcagcat tcacgttcgg acggagttcc
     1621 tgacctatgt cccctactat ttatgatatt gccagggttg caggcgtatc aaaatccacc
     1681 gtatcacgcg tgctgaataa gcaaactaat atctccccgg aagcgcgcga aaaagtgtta
     1741 cgggccattg aagaattaca gtatcagcca aacaagctag cccgcgcgct gacctcttcg
     1801 ggttttgatg ccattatggt gatttctacc cgttcgacca aaactacggc gggtaatccg
     1861 tttttctctg aagttttgca tgccatcacc gccaaagctg aagaagaagg tttcgacgtg
     1921 atattgcaga cgtcgcacaa cccggcagaa gacttacaaa aatgtgaaag caaaattaag
     1981 cagaaaatga ttaaaggcat tattatgcta agttcgccag cggatgagtc attttttgcc
     2041 caactcgata aatacgatat tccggtggtg gtgattggca aagttgaagg tcaatatgcc
     2101 catgtttatt ctgtcgatac cgataatttt ggcgacagca ttgcgttgac cgatgcatta
     2161 attgaaagtg ggcatcaaaa tattgcctgc ctgcatgctc cgcttgatgt ccatgtttca
     2221 gtggatcggg taaatggtta taagcagagc ctggctgcgc ataatattgc agtgcgtgat
     2281 gaattgattg ttgacggtgg ttatacccat gaaacagcac tgcaagccgc gagagaatta
     2341 ttaagccagt caccgttgcc tgaggcggtg tttgccactg acagtctgaa attaatgagt
     2401 atttatcgtg cggcagcaga gaaaaatatt gctattccgc agcagttagc ggtggtgggt
     2461 tatagtaatg aaacgctgtc gtttatatta acgcctgcac cgggcggcat cgatgttccg
     2521 acgcaggagt taggtcaaca aagctgcgag ttattattcc gcttaatttc tggaaaaccg
     2581 tcaccacaga atattaccgt tgccacgcat atgacgctga aataaatacc agctaccttg
     2641 cctgaatgcc ggatgcgatg ctagcgcatc ttatccggcc tacatttata gatcggataa
     2701 agtactcgcg ccgcatccgg caatttagcc cattatcaga acgagtaatt tacgccgaca
     2761 cctgcataat ggaatttatc actgtctcct tcgtcgtgat cctgccactc aaacgcgtat
     2821 tccagcgaaa cggaaagtcc gttctggaaa tcataaccgt aaaataaacc tacacggtta
     2881 aaatcatggc cttcacgttc aatatcatcc tgccagtccc agttactcca gcgatccagc
     2941 ccaatgcgcg tatacggcgt caccgagtgg ttgccgagcg tcagcggcaa ataggcgcga
     3001 atttcttgcg tggaaaactc accgttattg cggctgtcgt ccatattgaa gccgcgctcg
     3061 agataatagt tcactcgcaa ggcaaccgtt tcgttgaagg tatattgcag acctgtttcc
     3121 gtttcgacac gggtatcagc gtaaccggta gtgttcagat cgttggcaaa tttatacatc
     3181 gacaaccaac cgttgaaacg taaatcgtca gtcagtttca catcccagtc tggcgcgatt
     3241 ttccagcgct gcatattcgc cgtgtcttta cccggttcat caacgtagtg ataaccataa
     3301 ttacggaaac cgccggtcag gccgaaactg aaatcatcgt tttcgaggaa ctgataatgc
     3361 acctccagct ccgggcgatc aaaccacgtt ccacgtttac ccgcgctata atctaccggc
     3421 ccttcctgat aataggccag agcaattctc cacggcccgt tggcggcatt aaaatagact
     3481 gaaggctccg c
//
LOCUS       DAJTYZ010000249         3490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000249 DAJTYZ010000000
VERSION     DAJTYZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.252"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="OO596_004131"
     CDS             complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="OO596_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6719814.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEYYLSILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
     gene            1018..1197
                     /locus_tag="OO596_004132"
                     /pseudo
     CDS             1018..1197
                     /locus_tag="OO596_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1214..1912)
                     /locus_tag="OO596_004133"
                     /pseudo
     CDS             complement(1214..1912)
                     /locus_tag="OO596_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2248..2433
                     /locus_tag="OO596_004134"
                     /pseudo
     CDS             2248..2433
                     /locus_tag="OO596_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2533..2742)
                     /locus_tag="OO596_004135"
                     /pseudo
     CDS             complement(2533..2742)
                     /locus_tag="OO596_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2792..2971)
                     /locus_tag="OO596_004136"
                     /pseudo
     CDS             complement(2792..2971)
                     /locus_tag="OO596_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1005 a          750 c          780 g          955 t
ORIGIN      
        1 ttaatcctgt aacttgaatc taattgtgaa aaactctccg gaagagtggt tatctgatta
       61 aaatgaaaat aatattcctt ttgtctgata ttgtttctat cagaaaattg aggaaaagta
      121 accagattat tgcgctgagc acagagaatt tccagtttat ccggtaagtc gggtaatgtt
      181 gtcagcctgt tataataaac atgtaaaaat ttcaattttt cgggaagagc cggtaagtta
      241 tatagcctgt tattttcaac aaataatgat gttaaatggt ttggcaaaac aggaaggttt
      301 tccagttgat tagaacgaac acttaaagtt gttaatgact caggaagttc tggtatcaca
      361 gaaagcctgt tattatcagc attaagttct ttgagaaatg caggcagctt tggcaatgat
      421 gttaatttat tattttctac aatcaaagca gaaatatgct ttggtaattc gggaagagac
      481 gtcaaattta ggtctgataa atttaatact gcttcttgat tctgaaagca ctgggagagt
      541 ctgttaaaag caatgcctcg ctcttccccg ggagaagagt tcttttccca ttcagtcagg
      601 atgctcagat aatattcatt actggttgag tttgagttaa tagaggggga gcaagaaact
      661 gatgagtgtg tattatttac agagaacata atgctaggtc aaccttatat ttgtgagaat
      721 aaatatttaa aattgtatat ttatacaaat ttcacttacc atttccgata ttaagtttta
      781 atagtttcca ttttacacat aacagaagtt agttgtaatt tatttattga ggttttctat
      841 ggaattcttt ttttattgtg actgcagtca ataataacac tgaattcaac attccagtga
      901 gcaaggaaat gtaatccgag taagtatatt tgttttacat catattttag ccaagctcat
      961 ttattcctat tatagaatga taaattaaaa ttactgtaaa aaaacgatac acatttaata
     1021 cgagggaggc cggcagaaaa acggcttgcg gtaaggcagg agaaatgtgt gccgcgaatg
     1081 aagtccttgt atgactggat aaagctccag aggaaaactt tgtcaaacca tgctgaaatg
     1141 gtaaaggcgt tcaactatat cctgaatcac tggaatgcac tgaatgagtt ttgccgggta
     1201 gtacttccaa tcattagaat atgtgctttt caatgaagga accgatgact ttttcgtgga
     1261 tttccacgga acgcgagaag cagattgttt tacgtgccag ccgcttgatg cgggggcgga
     1321 gggtcaggtt gttacgttca tacgttgcgt gaagattttg ccggtcaggt gtttctcctc
     1381 cggcacttct ctggcgtagc tgccccagtc atcgctggtc accatgccac catgccgata
     1441 cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg tggcccgcaa
     1501 gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca gtgttgttgc
     1561 gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc aacatcagcg
     1621 tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc cgtatgacgg
     1681 tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg ttatgtgcca
     1741 tttcaacgat ctgctcctta acgccgggtt ttctggcttc ataagagtaa gtgagctgga
     1801 aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta cggccatgtc
     1861 gataaacttc atcggaatga cagcgcggac aatgaacagt aacggtagcc atggaaaacc
     1921 ttaaatacca gtagattacc agaaacttct gtcgattgaa gacatcaccc gaagtttgaa
     1981 ttcattcgaa tactttttac gaggttcttt tcgccagtcc tgtaattcca tacttaggtg
     2041 ttcgtctatg tcagatgggc gtctaagtta ccaattcttg tctaatggct acatacggtg
     2101 gtcggtttac gcttaccgca ggacaggtgg taactgaaaa atagtttgct gaggcgactg
     2161 gaaacagaag ggcaagatcg attccgacag cagtgatggt cagctacctc atcgataact
     2221 ttgcaacagt gcccttcttg aagcatcatg cttcatgaag aaaaactggc acgtcatcaa
     2281 cgtaaacagg cgatgtatac ccgaatggta gccttcccgg cggtgaaaat gttcgaagag
     2341 tatgacttca cgttcgccac cggagcaccg cagaagcagc tccagtcgtt acgctcactc
     2401 agcttcatag agcgcaatga aaacatagtg ttacagggga catctgacat cacaaatccc
     2461 cgggtgggga tttgtgtata agagacagcc catggaggaa ggggatatat cgcagctctg
     2521 acgactcact ccttatttac ccgggaaata cttctccagc aatgcataca ggatggcagc
     2581 ggtttcagca tccattatgc cgtcataatt tttctgccgg aaatgaagct gaaaagcgcg
     2641 gacaagcgcc ttatatccgt catccgtctc cacaccagaa atactgtatc cataggtctt
     2701 cagccgtgac acgatatcaa ccttatccgg aatgccctgc gattcaaaaa ataaatattt
     2761 agtcagtaat tttatttttt tcctgaaaaa attactcttt ttcgaactcc agtgagcgtt
     2821 cacgactcat cagtgtgaaa atgatggcaa aaacagcaga cagtatcagt acgataaatg
     2881 tggcccccca tcccagataa tcgacaacaa gtcccattcc cagtgtcgca acaattgagc
     2941 cgaagatata ggaaaatgag cctgacagcc ctaacagaat aagtttacgc gtaaatcgcc
     3001 cggaatccag aagagcatta agtatcacat aaacaggttc cataccagtc ctgaccgcat
     3061 caggacgcca gtaaataaaa tctcgtagtc caccggcatc agagacaaga gtaatcgtcg
     3121 ttaccggtat ttcgactggt ttgtactggt gtgagcggac aaaactactg tacgtctctc
     3181 atacacgctc gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgactgcggg
     3241 taaacccttg tcgggaccac tccgaccgcg cacagaggca ttttcatcgc tgacaggccg
     3301 catggcctcg cagagccgga gggggactgg tgatattcgg acgaccgtac cggcttgcgc
     3361 cgggcttcgg cggttttaca cctgtgctac atgtaaccac cgtatgccgc cttatatgcg
     3421 ctgactcgca ttatttgcct tgttccgtct gaaagtaatc actatgatta aatatgatta
     3481 acaactaatc
//
LOCUS       DAJTYZ010000250         3479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000250 DAJTYZ010000000
VERSION     DAJTYZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.253"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..493
                     /locus_tag="OO596_004137"
     CDS             <1..493
                     /locus_tag="OO596_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6719815.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="OO596_004138"
     CDS             493..1083
                     /locus_tag="OO596_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR6719816.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1070..2110
                     /locus_tag="OO596_004139"
     CDS             1070..2110
                     /locus_tag="OO596_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6719817.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYPPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2113..2658
                     /locus_tag="OO596_004140"
                     /pseudo
     CDS             2113..2658
                     /locus_tag="OO596_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2682..>3479
                     /locus_tag="OO596_004141"
     CDS             2682..>3479
                     /locus_tag="OO596_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6719818.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSEIAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYDIIIPDTRFEGVLSIRWTDA"
BASE COUNT          894 a          785 c         1004 g          796 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattaccccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctgtgttggc gacattgctg tatacgacga ctcaaagccc ggcacgctga
     2941 atgactttct gattgctcct gatgagggcg acctgaaacc ggatgtcgtc aaacgctttg
     3001 aggaaatggt ggcgcaggcg cagcagagcg ccggggccgc agccggaaac gcacagcaga
     3061 cggcgcagga tgtggcggca gccgcaggtt atgcccgcgc agcagagcag gccaaaaatg
     3121 acattgatgc tgcgctgacc ggcactctga aaacggctaa ccatctgtct gaaatcgcag
     3181 cagcaggcga aaaggcacaa cagaagtccc gggataatct ggggctgaaa agtgcggcca
     3241 cgatggaagc acagagcgac atttacgacc ggacaaaagg ccgtctggcg atacccggcg
     3301 cattcggctt tgggtgtgct tttctgcctg aagatgttat ccgttttgac actaagagtg
     3361 atttcctggc ctgggtaagg aatgcgctgc caggtgaata ttccgttgct ggcccctacg
     3421 acatcatcat acccgacaca cggtttgaag gggtgctcag catccggtgg actgatgca
//
LOCUS       DAJTYZ010000251         3400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000251 DAJTYZ010000000
VERSION     DAJTYZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.254"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..171)
                     /locus_tag="OO596_004142"
     CDS             complement(<1..171)
                     /locus_tag="OO596_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HCR6719819.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAV"
     gene            415..672
                     /locus_tag="OO596_004143"
     CDS             415..672
                     /locus_tag="OO596_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCR6719820.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(722..1672)
                     /gene="uspE"
                     /locus_tag="OO596_004144"
     CDS             complement(722..1672)
                     /gene="uspE"
                     /locus_tag="OO596_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6719821.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1824..2576)
                     /gene="fnr"
                     /locus_tag="OO596_004145"
     CDS             complement(1824..2576)
                     /gene="fnr"
                     /locus_tag="OO596_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6719822.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2771..3286)
                     /gene="ogt"
                     /locus_tag="OO596_004146"
     CDS             complement(2771..3286)
                     /gene="ogt"
                     /locus_tag="OO596_004146"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6719823.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3297..>3400)
                     /locus_tag="OO596_004147"
     CDS             complement(3297..>3400)
                     /locus_tag="OO596_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCR6719824.1"
                     /translation="PYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT          889 a          812 c          803 g          896 t
ORIGIN      
        1 aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata aaaaaccttt
       61 tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg cgcagctacc
      121 gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca ttgttttatc
      181 aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat tttaccgcga
      241 caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct aactaatcaa
      301 tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac ggtgaaatgt
      361 catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta catcatgatt
      421 atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt ggcggttttc
      481 attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga acagtacaat
      541 attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat gatttttgtt
      601 tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt ccttggcggc
      661 gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta ttgaagagtc
      721 gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca ggtttaatga
      781 ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg aggaatgctg
      841 ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt aagtgctccg
      901 ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg ttttcattaa
      961 tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga atggcatcgt
     1021 tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc gttactgggt
     1081 aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg atcgtctctt
     1141 tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga ttcaccgcca
     1201 ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc gggcttgggc
     1261 atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga cggtcatgtt
     1321 ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga atgatggctt
     1381 cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca ttgagataat
     1441 attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga cgcatagcgg
     1501 tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag atcggcaaaa
     1561 aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc aatgctggtt
     1621 ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc atacagggtc
     1681 tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat ctggtgaatg
     1741 aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga tatgacagaa
     1801 ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc aagctgggcc
     1861 agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat accgcttttc
     1921 tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata gttaccgata
     1981 tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg ggcaaaacga
     2041 cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt cgacaacagc
     2101 aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat ctgctgacgc
     2161 agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc acataccatc
     2221 gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc gtcaaatccc
     2281 accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc agtgatggta
     2341 taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc accagcctta
     2401 aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc aagctgatca
     2461 agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca atcctggcaa
     2521 tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg gatcataggt
     2581 ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag caagtaagtc
     2641 taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt ctatctattt
     2701 gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc tggcacaaat
     2761 tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct tttcgctgaa
     2821 ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg caaggtacga
     2881 cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg ccagggcggc
     2941 ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata gtgcgtagag
     3001 ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc gtatcaataa
     3061 tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct gggttggtgg
     3121 cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc accatgcgtt
     3181 cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa atcacccaca
     3241 gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt tctctcttaa
     3301 gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca cgccagcaac
     3361 atcagcagcc ataccaccaa aaagataagc ggatagggca
//
LOCUS       DAJTYZ010000252         3362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000252 DAJTYZ010000000
VERSION     DAJTYZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.255"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..879
                     /gene="adeQ"
                     /locus_tag="OO596_004148"
                     /gene_synonym="yicO"
     CDS             <1..879
                     /gene="adeQ"
                     /locus_tag="OO596_004148"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6719825.1"
                     /translation="IGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFII
                     TVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLEL
                     AGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTS
                     SVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLM
                     TSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLC
                     VVVVASLFALKIILVD"
     gene            932..1384
                     /locus_tag="OO596_004149"
     CDS             932..1384
                     /locus_tag="OO596_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6719826.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1595..2775
                     /gene="nepI"
                     /locus_tag="OO596_004150"
                     /pseudo
     CDS             1595..2775
                     /gene="nepI"
                     /locus_tag="OO596_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2816..3109)
                     /locus_tag="OO596_004151"
     CDS             complement(2816..3109)
                     /locus_tag="OO596_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719827.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
BASE COUNT          685 a          754 c          889 g         1034 t
ORIGIN      
        1 attggattat ttatcgcctt aatgggatta aaaaatactg gcgttattgt cgccaataaa
       61 gacacgctgg tgatgattgg cgatttaagt tctcacggcg tgttgttagg tattttaggg
      121 ttttttatta taaccgtgtt gtcatcacgt cattttcatg ccgcggtgct ggtttctatt
      181 gtggtgacgt cttgctgtgg attatttttc ggtgatgttc attttagcgg cgtctattcc
      241 attccgcctg atattagcgg cgtcattggt gaagtagatt tgagcggcgc gttaacactt
      301 gaactcgccg gtatcatttt ctcctttatg ctgatcaacc tatttgattc atcaggaaca
      361 ttaattggtg taactgataa agcgggctta atagatggta acggtaaatt ccccaatatg
      421 aataaggcgc tgtatgttga tagcgtcagt tcggtggcgg gtgcgtttat cggcacctcg
      481 tctgttactg cctatattga aagtacttct ggtgtggcag tcggtggccg cacggggctg
      541 actgcggttg tggttggcgt tatgttcctg ttggttatgt tcttctcccc gctggtggcg
      601 atggttcctc cttacgcaac cgccggagcg ttaatctttg ttggcgtgct gatgacttcg
      661 agcctggcgc gcgttaactg ggatgatttt accgaatcgg tgcctgcgtt tattaccacg
      721 gtgatgatgc cctttacttt ctcgatcacc gaagggattg cactcggctt tatgtcgtac
      781 tgcatcatga aagtgtgcac cgggcgctgg cgcgatctga acctgtgtgt ggtggtggtc
      841 gcatctctgt ttgcactgaa gattattctg gtggattagt aactggcgag ggtttccaga
      901 tatcatgagt tctgataacg caggagaact catgatctgg ataatgctcg ccacgctggc
      961 ggtagtgttt gtggttggtt ttcgggtgct gacatccggg gccagaaaag cgattcgccg
     1021 tctcagtgat cggctgaata tcgatgtcgt acccgtggag tcgatggtcg atcaaatggg
     1081 aaagtcagcc ggtgacgaat ttttacgtta tttgcatcgc ccggatgagt cgcacctgca
     1141 aaacgccgcg caggtgttgc tcatctggca aattgtcatt gtcgatggta gcgaacagaa
     1201 cctgctgcaa tggcatcgga ttttacaaaa agcccgcctt tccgcgccga ttaccgatgc
     1261 tcaggtcagg ctggcgctgg gttttctgcg cgaaaccgaa cctgaaatgc aggatattaa
     1321 tgcttttcag atgcgctata acgcgttctt tcagcctgcc gagggcgttc actggctgca
     1381 ttaaaaccgc ttcgtcttat cagcctacag accgcgcctg acctgtcggg tggataagac
     1441 gttcacgccg catccggtaa tctttgccct gttccgtttc gatcttaact ccagctcaag
     1501 caacaccgta aaagttgcaa aaacgttaaa tgcgtcacac atttcaatgt taaagtggtc
     1561 ggcttttccc ctgaaacatg ccacgggtaa caccatgagt gaatttattg ccgaaaaccg
     1621 cggcgcggat gccatcaccc gaccgaactg gtcagccgtt ttctcggtgg cgttttgtgt
     1681 cgcctgtctg attatcgttg agtttttgcc cgtcagtttg ttgacgccaa tggcccagga
     1741 tttaggcatt tcggaagggg ttgccgggca atcggtgacc gtgaccgcct ttgtggcaat
     1801 gtttgccagt ttgtttatta cccagacaat tcaggctact gaccgccgca acgttgttat
     1861 tttgtttgcc gttttgctga cgctctcctg cttgctggtt tcctttgcta actcattcag
     1921 tttgctttta atcggtcgtg cctgtctggg gctggcgctg ggcgggttct gggcgatgtc
     1981 ggcgtcgctg accatgcgtc tggtgccgcc gcgtacggtg ccgaaggcgc tgtcggtgat
     2041 cttcggcgcg gtttctattg cgctggtgat tgccgcgccg ttgggcagtt ttttaggcga
     2101 gcttatcggt tggcgcaatg tctttaatgc ggcggcggtg atgggcgtgc tgtgtatttt
     2161 ctggattatc aaatcattgc cttcactgcc aggcgaaccc tcgcatcaga aacaaaatac
     2221 tttccgctta ttacaacgtc cgggtgtgat ggcagggatg atcgccatct tcatgtcttt
     2281 cgccgggcag tttgctttct tcacatatat tcgcccggtg tatatgaacc tagcgggatt
     2341 cagcgtggat ggcttaacgc tggtgctgtt gagttttggc attgccagct ttatcggtac
     2401 gtcgctttcg tcattcattc ttaaacgttc ggtaaaactg gccttagcag gcgcgccgtt
     2461 aatactggct gtgagtgcgt tggtgctgac gttgtgggga agcgataaaa tcgttgctac
     2521 cggcgtggcg attatctggg ggctaacttt tgcattggtt cccgtcggct ggtcaacgtg
     2581 gatcacccgc tcactggccg atcaggcaga aaaagccggg tctattcagg tggcggttat
     2641 tcagcttgtg gcgcggcaat cggcggttat gcgctggata atattggtct gacttcgccg
     2701 ttgatgtttt ctggcacatt gatgttgctg actgcattgt tggttactgc aaaggtgaaa
     2761 atgaagaaat cctgatatca ggtaaagaag cgttggtcag tagttgttgc tgttcttaca
     2821 tatccgggca ttctacctta cccaacgctt cccaataatt gctctgatta ttacgttcca
     2881 tcgccacgat ggcctcgcgt atatgttgtt tattgttttc atctgacaac attaactttt
     2941 cccacgcttc atctgataat gacgcctgtg gcgtgcaaat tttacgtaaa tcttgtaata
     3001 ccgtggtttt ctcttttgcg gactgcagtg agctaaaagg cgactcggcg tatacgatgc
     3061 ctggcatagc gaaaaaagtg aaaataagca gtagtgtcgt tggtttcatg gattcaacct
     3121 tttacaaatg tgtatcgaat gaagcataag tgtagtttgc tttcatcgct ctcatcatgc
     3181 tgtacgaaac gcgcttgcgt ggtcagtaag aagtgccaga ctttatattc cacttttatt
     3241 cctttttatt cttatcgata gcgtttcgtt ttttaaaccg cagcgacctt accgctatag
     3301 tcaggtaatc attaataaaa ggataaaaaa atgaaactga caacacatca tctacggaca
     3361 gg
//
LOCUS       DAJTYZ010000253         3344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000253 DAJTYZ010000000
VERSION     DAJTYZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.256"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..628
                     /locus_tag="OO596_004152"
     CDS             <1..628
                     /locus_tag="OO596_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6719828.1"
                     /translation="IYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHL
                     DIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALH
                     GQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKL
                     MRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(683..781)
                     /locus_tag="OO596_004153"
                     /pseudo
     CDS             complement(683..781)
                     /locus_tag="OO596_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(827..1009)
                     /locus_tag="OO596_004154"
     CDS             complement(827..1009)
                     /locus_tag="OO596_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6719829.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            1108..1998
                     /locus_tag="OO596_004155"
                     /pseudo
     CDS             1108..1998
                     /locus_tag="OO596_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2001..2534
                     /locus_tag="OO596_004156"
     CDS             2001..2534
                     /locus_tag="OO596_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6719830.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2563..3090)
                     /locus_tag="OO596_004157"
     CDS             complement(2563..3090)
                     /locus_tag="OO596_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6719831.1"
                     /translation="MMHLKNIKAVNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(3092..>3344)
                     /locus_tag="OO596_004158"
     CDS             complement(3092..>3344)
                     /locus_tag="OO596_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6719832.1"
                     /translation="GDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYKNVWRDYGFGDA
                     SGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          944 a          735 c          728 g          937 t
ORIGIN      
        1 aatttacaca ccacttaccc taaagcttta tgactggtgg gttttgggag tatcaaatcg
       61 gcttgcatgg ggatgtccta caaaggaaca ccttcttcca cactttctgg aacatttagg
      121 taacaaccat ctggatattg gtgttggaac tgggttttac cttactcacg tacctgagag
      181 tagtctgata tctttaatgg atttgaacga agctagcctg aacgcggcat ctacaagggc
      241 tggggaatca aaaattaaac ataaaattag ccatgatgtt tttgaacctt atcccgcggc
      301 gttacatggt caatttgatt ccatttccat gttttacctt cttcactgcc tgcctggaaa
      361 tatatctaca aaaagctgtg taatacgcaa tgcggcgcag gccttaactg acgatggaac
      421 tctatacgga gccacaattc ttggcgatgg agttgtgcac aatagcttcg gtcaaaaact
      481 gatgcgcatt tacaatcaga aaggcatctt ttcaaacaca aaagattccg aagaaggctt
      541 aacacatata ctctcagagc atttcgagaa tgttaaaacc agggttcaag gtactgtagt
      601 aatgttttcc gcttcaggga aaaaatagca tccaaccgca gcacgttctt gcttaagacg
      661 tgctgcggca taatcccaat gattactccc tgacagggtt cgtaggccac tcaatatcag
      721 gtgcagttga tgtatcaaca cggttcagca acacccgata ctttttctag gcttccacca
      781 ccagcacgac aagatgccgc atacagtgcc ccagtcagtc cagttttcag acaaccagtg
      841 cgtcaccttt ttgaaggcgc tttaaagcac gttttaatcc aggtcggcct gtccttgttc
      901 cgcttaattt atcttcaaat atttgttcat atcctgcaca aacaagagcg tttcgttgca
      961 ggtctgtatt ctggtcattt gttgataccc ttacatagcc aatcagcacg ctgaatctcc
     1021 cgtccaaaag cacaaatcat gccatgcagg ccagaaaccg ccattatcta aaacctcggt
     1081 ttacgagaac tcggcacaag cggggagaaa ataccgttac tcagtacagc gaatacctgg
     1141 actaatcgac aaacattcag cggtggcctt tctggtgaac tatccggcaa tgcttctaca
     1201 gctgcaaaat taaaaactgc caggaaaata agcaatgtgg cttttgatgg ttcctccgat
     1261 atcacattaa aagcaagtca tgttggtgcg tttgccttag ggaaaacagg aagcaccgtt
     1321 gcgaatgata aagcagttgg atggaactgg agtagcggag cctataacgc aactattagt
     1381 ggtgcatcaa cgttaattat tcatttttat atgggagaag gaagttgtcc tgcagctcag
     1441 tttcggatta attataaaaa tggcggtatt ttttatcgtt cagcccgtga tggttatggt
     1501 tttgaagccg actggtccga attttacacc accagaaaac cttcagcagg agatgttggt
     1561 gcactgccgt tatctggtgg tcaactgaat ggtgcactgg gtatcggaac atccagtgct
     1621 cttggcggta attcgattgt attgggtgat aatgacacgg gctttaaaca aaaaggcgat
     1681 ggtaatctgg atgtttatgc taataacgtc catgttatgc gctttgtctc cggaagcatt
     1741 caaagtaata aaaccataaa tattacggga cgtgttaatc cctcggatta cggtaacttt
     1801 gattcccgct atgtgagaga tgtcagactt ggcacacgag ttgtttagac catgaagaaa
     1861 ggcgtgatgt atgaaaaatc aggccatgta atcacggggc ttggtattat cggtgaagtc
     1921 gatggtgatg atccggcagt attcagacca atacaaaagt taattaacgg aacatggtat
     1981 aacgtatcgc aggtataatc atgcagcatt taaaaaacat tgtcgcaggt aatccaaaaa
     2041 ccgttgaaca ataccagcta acaaagaatt ttgatgttat ctggttatgg tccgaagatg
     2101 gaaaaaattg gcatgaggaa ttaaaaaact ttcaggagga tacaataaaa ctggcttaca
     2161 cagttgaggg aataattatt gctatagaca aggatgtatc agcaattaat ccagaaggtt
     2221 taagtgtcgt tgagttgcct gatattacag caaatcgccg ggctgatatt tcgggcaact
     2281 ggatgttcaa agatggtgcg gtgataaagc gaacttatac cgaggaagag caaaggcaac
     2341 aagcggaaaa tgaaaagcag agcctgctgc agctcgtcag ggataaaacc cagctgtggg
     2401 actctcagct acagctgggc atcatttccg atgagaataa acaaaaatta accgagtgga
     2461 tgctctatgc gcagaaggtt gaatccacag acacctccag cctgccagta acgtttcccg
     2521 aacaaccaga atgagagaag gcccgctatc gggccttaat ttttattcag gcttttgtgg
     2581 ccattcagga tttgccgtat ccacacggct gaccagaaca ctgtagcgtt cccatgcttc
     2641 cagtcgtgtg cgttcctcgt ctgttgccat attcagcctg acagctcgtt ccagcggctg
     2701 gatgactgat tcagcttcgg aaagcaatgc ggccttttgt gattcggcct gttgttgcag
     2761 ttcctgttct gtatattccc tctttactac tttaccatcc agaaacatcc attttccgtt
     2821 attgtctgcg tgacgatttg ccgtaatatc aggaacttca actatgctta gcccttcagg
     2881 attaatagtt gaaacgtctt tactgacagc aacgatgatg ttgttctcat cgtaagcaat
     2941 ttttatggtg tctggctgaa aattcttttg ttcctcatac cagtttttgc catcttcgca
     3001 aaagagccat accacgcccg cttttttagt tagctggtat tgttccggtg ttttcggatt
     3061 cacggcctta atatttttca ggtgcatcat cttaaacact ccccacgttg taccatattc
     3121 ccccaatcaa tttctgaatt ggccttctgg caacagtgtc gacaatatca tccgcattgc
     3181 tgttaattgc ggctgttaaa acataacctg acgcatcgcc aaagccgtag tcgcgccata
     3241 cgtttttata ttcaatgctg ccaagacgaa tatcccgaac gacatgatta tgcagccatg
     3301 tgcttaacca gttgttttcc cacactgaac cataaatatc gcca
//
LOCUS       DAJTYZ010000254         3333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000254 DAJTYZ010000000
VERSION     DAJTYZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.257"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(194..784)
                     /gene="caiE"
                     /locus_tag="OO596_004159"
     CDS             complement(194..784)
                     /gene="caiE"
                     /locus_tag="OO596_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6719833.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(790..1575)
                     /gene="caiD"
                     /locus_tag="OO596_004160"
     CDS             complement(790..1575)
                     /gene="caiD"
                     /locus_tag="OO596_004160"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6719834.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(1684..3237)
                     /gene="caiC"
                     /locus_tag="OO596_004161"
     CDS             complement(1684..3237)
                     /gene="caiC"
                     /locus_tag="OO596_004161"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6719835.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          759 a          950 c          810 g          814 t
ORIGIN      
        1 accacgcagc ctggttgctg gatgcaatgc tggcgcatct tatccagcct acggttgctc
       61 tccagccggg ttggataagg cgtttatgcc gcatccgaca gctatcgccg gatgcgatgc
      121 tggcgcatct tatccggcct acggttgctc tccagcagag ttggataagg cgtttacgcc
      181 gcatccggca agatcaccgt ttaggcgtca catccgtcgt cccctgcaaa cgggggcgat
      241 tttcctccat ttgcctcagt ggctgtgttt catgtaacga tgcatgacag cgcccgacaa
      301 gatcctgata ctctttggta ttcaaccgtt tccagtgtaa ctcgtcgtca ctaacactgc
      361 gtacagcgcg ggctggcgta cccatcaaca actggcgttt ctcgccgtga aagcctgctt
      421 tgacaaagct catggcggca acaatgctct cttcgccaat gaccgcgcca tccataatca
      481 cgctgttcat cccgaccaat gcatcgcgac caatcacaca accatgcagg atcgctccgt
      541 gcccgatatg gccgttttcc ccaacgatgg tgtcagtgtc gcagtagcca tgcataatgc
      601 agccatcctg aatattggct cccgcttgca cgatcaaccg cccgtagtca ccacgcagtg
      661 aggcgagtgg gccgatgtag acaccggctc ccacaatcac atcgccaatc aagacggcac
      721 tgggatggac aaacgccgtc gggtgaacca ccggaattaa cccctcaaag gcgtaatagc
      781 tcacggttgt taacgtcctt tccacaccgg atcgcgcttc tcggcaaacg ccagcggccc
      841 ttcaatggca tcctccgaat gcagaaccga cggatagtgt ttcaacacgc cgctgcgaat
      901 atagcgatac gcttcttcta ccggcatttc gctggtggtg cggaagatct ctttcagcgc
      961 cgcaatcgcc agcggggcgc tgttaaccag ctgctgcgcc agttcgcggg cgttatccat
     1021 cagttccgcc tggctaacca cgcggttgac tatcccccaa cgcagcgcct cttctgcgcc
     1081 cattcgtctg ccggtcatca ccatttcatt gacgatggca ggcggcagga tcttcggtag
     1141 acgcagcacg ccgccgctgt caggaacgat gcccagcttg gcttccggca gggcgaagct
     1201 ggcgttatcg gcacaaacaa taaaatctgc cgccagcgcc agttcgaaac cgccgtcaaa
     1261 ggcatagccg ttcacagctg cgataaccgg tttgtcgaga ttgaaaattt cggttaatcc
     1321 cgcaaatcca cccgggccaa agtcagcatc cggtgcttcg ccttctgctg ccgcttttaa
     1381 atcccagccc gcggaaaaga acttctctcc ggcaccggta ataatggcga cacgtaattg
     1441 cggatcgtca cggaaattta gaaatacttc gcccatttca aagctggttt ttgcatcaat
     1501 agcattcgct tttggacgat caagggtaat ttccagaatt gatccattgc gggtcagatg
     1561 taatgattca ctcattcctt ttctccattt ttgctttttc agggacgaca acatccctgc
     1621 aaaaaatgca tattgtttta tagcgtgatt attagttggc agggtagttc cctgctgttt
     1681 catttatttc agattctttc taattatttt ccccgagcaa ttacgtggca gatcttttct
     1741 gatctccaga taagagggca ctttaaattt cgccatattt tgttcgcaga agcggaaaaa
     1801 ttcctcttcg ctcaatgttt caccttcatt cagcaccaca aatgctttga tggcttcatc
     1861 gcgaatcgaa tctttaatac ccacaaccac gatatcctga atttttggat gggtggcgat
     1921 gatattttcc agctccacgc aggagacatt ctcgccaccg cgtttgatca tattgcagcg
     1981 gcgatcgacg aaataaaaaa agccctcttc gtcgcagtat ccggtatcgc cggtatgtag
     2041 ccagccgtcg gcttccagca ctttcgcagt ggctttcggg ttgagaaaat actctttgaa
     2101 gatggttttc cctggtacgc ctttaataca gatttcaccg atctcacctg ccgggagcgg
     2161 gcgattgtga tcgtcgcgga tctccgcttc gtagcaaaac cccgcccgac caatcgacgg
     2221 ccagcgtcgt ttatcgccag ggcgatcgcc gataatgccc acaatggttt ccgtcatccc
     2281 ataagacgtc agcaagcgaa cgccgaagcg ttcacaaaac gcatcttttt cctgctccga
     2341 caagttgaga taaaacatca cttcccgcag gcggtgttgc cgatcgttcg ctgaaggcgg
     2401 ctgcgccatc aaggtacgga tcatcatcgg aatacattcg gtaacggtgg cgcggtactt
     2461 ctgtacctgt ccccagaagg cgcgggcgct gtatttctcg accagcacaa aggtggcccc
     2521 ggcagaaaac gccgccatcg ccgcagtaca ctggcaatcg atatgaaacg caggcattac
     2581 cgtcaggtag acgtcatcgt cacgcagtgc acactgccag gcggagtaat atccagcgaa
     2641 gcgcaggttg taatgggtaa tcaccacacc tttcggtcgg gaggtggtgc cggaggtgaa
     2701 gagaatttcc gccgtatcgt cagtcgatag cggcggtgca tagcacaagg tggcaggttg
     2761 ttgatttttc agttgagtaa acgaactcac gccatcatca gcgggaagtg ccacatctgt
     2821 caggcaaatg tgccgcaatt gagtggcatc ttcctgctga atctgttgat acataggata
     2881 gaattgcgca ctggtcacca gcaggcacgc ctggctattt tgcaggatcc acgcgctttc
     2941 ctcgcgcaac aggcgggcgt taatcggcac cataatcgcg ccaatttttg ccagcccgaa
     3001 ccagcaaaag ataaattccg ggcagttgtc gagatgtaga gcaaccttgt cgcctttgcg
     3061 aatccccagc gtataaaaca ggtttgtcgt gcggttaatc tcctgattta actcaagata
     3121 actataccgg ttaacgactc cgccgctgga ttcacaaatc agcgccgttt tatgaccgta
     3181 aacgtccgcc agatcgtccc acatttgacg tagatgttgt ccgccaatga tatccattgc
     3241 acctctatcc atttttgttc gtttgttatt gggcgggcgc tagtcaggca agccgactga
     3301 cgccacgcgt ttagtcctca actttggcca gac
//
LOCUS       DAJTYZ010000255         3282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000255 DAJTYZ010000000
VERSION     DAJTYZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.260"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..145
                     /locus_tag="OO596_004162"
                     /pseudo
     CDS             1..145
                     /locus_tag="OO596_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(175..3087)
                     /locus_tag="OO596_004163"
     CDS             complement(175..3087)
                     /locus_tag="OO596_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719836.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPKGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3090..3206
                     /locus_tag="OO596_004164"
     CDS             3090..3206
                     /locus_tag="OO596_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6719837.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
BASE COUNT          827 a          882 c          798 g          775 t
ORIGIN      
        1 ggcaaaggcc tcccggaagt tcagcccggt cagttaccgc gcacatggcc tgcccgtgtc
       61 agaaaatctg ctggagcagg atttttacgc cagtggtccg aaccagaagt gggcgggaga
      121 catcacgtac ttacgtacag atgaagttcg gttacatcct gtcagcacag aacctcacgc
      181 tttttgagcg gcacgacgga tcccccccac ccagtatccg acagactttt cactgcgttc
      241 gtaaaaaccc ttcttccctc ccagcatatc cactaattcc ggtgacatat cctgcagata
      301 agtctgcaaa tcatgcccca gatcttccgg tgactgcaac tcataaaccg cgcgccacac
      361 atcaccctgc cttattttaa gccccgggtt aagaatatac tgtccgtggt tcagcctcag
      421 aaaaagcttt tttccataag gatttttgct gttaacttca tgttgagaga gctggctgct
      481 gatataactc cttcgtttgc ggcgttccgg cagcaacgct tcaggaagat gaaccacaca
      541 ctctgccagt cgtgccgcac tgaaggcttc cgaaaaaaac agagcacttc ccagatgcgt
      601 gtggaacagc gcctgcatca ggatcaccag aaacattgcc atctgccggt tgtccagttt
      661 gatgaggcgt ccttccacct gcagtgacag actttcaggt ttcagcaggg tatacaactg
      721 cgcacttttc tgttgcgcat aacggggcgt actgaccgcc gctgacagaa cttgatgata
      781 agccgccagc ccgtcattac cggtcagcaa gggatctgca ccacgctcca gcagtaactg
      841 aaccagtgca atattacctg cccgggctgc tgacatcagc ggagtaagat taaacggcga
      901 acggtgatca acaccataac gttcaatatc ccgcaacacg gcaaccgggt tattcattga
      961 gtatggccgg aaatacttca gctccagtaa ctgcagagtc ttcgttctgg gctgccttgt
     1021 gggcttaaaa tcttcccggt acagtgcatt atacagcggg aacagggaat aaatcagact
     1081 gtactccagc gcctgcagtt gtatggtttt atcggctgta cctgcgagga tatgctgttt
     1141 ccactgtcgt gcatcctctg cggtgataat ctgccatggc atctcagatg tctgcaggat
     1201 ccggctacgg atggtgtccg cctgctcctg cttcccctgt ttttcaagca gatgtgcggt
     1261 tttctgccac tcatcacggc tggaaatctc ttcctgcaga ttcagaggct cttcctgatg
     1321 cgtgagcgca agcagactcc acagaggatg ctccacctgc tgttcaacaa gatacacatt
     1381 gtgtgtagca cgggtgagcg caacatacag cgcgttggta aaaaatttat agatttccgc
     1441 tgaacgatcc tgcctgtccc gcggacggga ataacgggcc tcgccctcaa gatcagccgg
     1501 tgtaagaccc tcgcaaatat catcaaatgc ctgccgggca gcgcttacta tattgtaaag
     1561 gatcaccgtt tcgtattcca gccccttaac ctcctgaacc gaaaagacca gcggcgtact
     1621 gaaccagcat cgcgcctgtg ctttctgttc cggatgcatc acaatgaccg ctacacgatt
     1681 cgaaagtgag gttttggcat tcagttcctg ctttgtctct tccctgtcgt ccagcaaccg
     1741 gatggtccct tctgcctgac cgcagcttct gacaaaatgg tgactttccc gatcaatagc
     1801 actaaagcgg acctgtttga gtctcagcaa ccggttagcc agtgcagtga cgtggtgccc
     1861 gttacggtaa ttcgcctgca ggatattaac ggttgtgtcg tttccctgtt gctgccggaa
     1921 aaagagactt ttgagcgaac tccatgagaa aaagctcgga tgaactatct gattggcatc
     1981 accacagagt aaaaactgac cgggatgacg tagcgttttc agtaccagtt gcagttgaac
     2041 aggcgtcata tcctgaactt catcaacaaa aatgacatca taacggggag ttgccaacga
     2101 aagatactga tgactgagca gattactgtc atactgatgc gactgtttca gccatgcgat
     2161 ataacgttca aacaggacat agaccgtcgg gcggtcctcc atgccataga gcgactgccg
     2221 tatccccagt gacagatacg cctcccggga cagcggccca ttcccggagg caaccgcacc
     2281 gatcacgccc ctgaactcct catacagggt atgggcagcc cccagtgcgg cgatagccct
     2341 gttgcggggc agccagtcgc tgaacgcact gaaagtcacc tccctgcctt tcggaatgcg
     2401 cagagtttcc agaaattccg tcagcgaaag aaagtcaata ttctgtacct cactcccttc
     2461 ccctgaagcg tcatagagag tacgcgcttt ttccaccaga aatgaggaaa ggctgagata
     2521 cagtatatca ccagcagcct gcttcatttt ttccagtacc agcgccgtct taccacttcc
     2581 cgccgagcca acaatcacca gcggtagcgg gtgttcgtac agtgcctgct gatcatcatc
     2641 aaaggacagc attttatcca ggcggtggaa acgcccatga gacggattga tatacgtcag
     2701 cgcttcagtc gcgatatcaa caggatccgg cactggttgt tgttgtaagc gcccttcatc
     2761 aatcaccacg tttctgcgaa gaaaacgtga agtgttatag gcatgattgc gaatatattc
     2821 cagaacaagg cagatagttt caccgtggta gcgatacagc gaaaacagta atcggtcccg
     2881 gatactgagc cgggcgcggt aaagattggt gtcaatttta cgaacatcag cctgaacaaa
     2941 gttatcggtg gcaatagcct gtgcaaattt tttataaccc gggatcgtgt caggattcag
     3001 agtatgataa agacagattt tcatggctgc cggtttccgt cgtgcaaaaa acatgctgat
     3061 tttacgaagg aatgccttac tgaacaacct tgcccggaat accccccgaa atcagatgga
     3121 acaatcagaa aaatgctctg cagttatgga ataaagaagg tggggtggta cgccgggagg
     3181 gcgatccgct ggaaatggcg cagtagctgc aggcctgtca tgatgccgga atagaagttc
     3241 gtgtacaggt taatgaaagc gtcaccccgt aaataaactc tg
//
LOCUS       DAJTYZ010000256         3216 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000256 DAJTYZ010000000
VERSION     DAJTYZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.261"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            750..1466
                     /gene="nanC"
                     /locus_tag="OO596_004165"
     CDS             750..1466
                     /gene="nanC"
                     /locus_tag="OO596_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6719838.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1486..2592
                     /gene="nanM"
                     /locus_tag="OO596_004166"
     CDS             1486..2592
                     /gene="nanM"
                     /locus_tag="OO596_004166"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6719839.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2657..>3216
                     /locus_tag="OO596_004167"
     CDS             2657..>3216
                     /locus_tag="OO596_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6719840.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMT"
BASE COUNT          975 a          659 c          725 g          857 t
ORIGIN      
        1 cttcagaagc gaccagttac aagccacaaa tcaggaaaca tatttcatgg tgagaattat
       61 gatcagcctg atggcgggta aacacttgtt ttcaccacgc tcactgagtt agcttgcaaa
      121 gcttcccttg tttagaccct aaataaattt tttataaaac aaacagttaa aagaaatcct
      181 ttagtttttg aagaccacct caagtgttcg tctggcttca catggcatct tcctcttaga
      241 aaaagatcga catattttgt gacacgaatt gcaaatctcg ttttgttgta tggcttgcgt
      301 gatttttgat ctggtataac aggtataaag gtgcaccgcg atagtcaatg agacagggca
      361 tctcgcaatc tatggcaaac atcacttcag ttctttctca tcgggtgatg aaaacgcact
      421 tcagtctgaa aggaatatga aaatgagatc aacagacact ctattttatg actctgggta
      481 aaatggattg agtaagtgat atagcttacg aacattcaaa tcaattaaac atcagaagag
      541 attttatact caggtattta atctgattct ctgtttattt aaataatgtg gaaagagaat
      601 tttcacagga gaccttatac aaaaatataa aataagctac ccgttgccaa agacactata
      661 agcctggcaa aaaatattac acaacataaa tgctaattgt ttatgcgggc tttgtattgc
      721 tttctgtatc ctacaaatga gtgaaattta tgaaaaaggc taaaatactt tctggcgtat
      781 tattactgtg cttttcgtca ccattaattt ctcaggctgc gacactggac gtacgtggtg
      841 gatatcgtag cggaagccat gcctatgaga ctcgactcaa agtcagtgag ggatggcaaa
      901 atggatggtg ggcaagcatg gaaagtaata cctggaatac cattcatgat aataaaaagg
      961 aaaatgccgc actcaatgat gttcaggttg aagttaatta cgcgattaaa cttgatgatc
     1021 aatggacggt gcgcccggga atgttaacgc attttagcag caacggcaca cgctacggac
     1081 cctacgtaaa actgtcctgg gacgcgacaa aagatcttaa ttttggcatt cgctatcgtt
     1141 acgactggaa agcttaccga caacaagact tatccggtga tatgtctcgt gataacgttc
     1201 atcgttggga tggatatgtc acttaccata ttaatagtga tttcaccttc gcatggcaaa
     1261 cgacgctata cagcaaacag aatgattatc gctatgcaaa ccacaagaaa tgggcgacgg
     1321 aaaatgcctt tgttctacaa taccatatga cgcccgatat tacgccatac atagaatatg
     1381 actaccttga ccgccagggt gtttacaacg gcagagataa tttatcggaa aacagttatc
     1441 gcattggtgt gtcatttaaa ctgtagtaga caggagacag tcacaatgaa taaaactata
     1501 atggcgcttg ctatcatgat ggcttcattt gccgcaaacg cgtctgtatt accggaaact
     1561 cctgtgccat ttaaaagtgg taccggagca attgataacg acactgtcta cattggttta
     1621 ggtagcgcag gtacagcatg gtacaagcta gatacacagg ccaaagataa aaaatggact
     1681 gcgttagctg cattccctgg tggaccgaga gatcaagcaa cctctgcatt tattgatggc
     1741 aatctatatg tgtttggcgg cattggcaaa aacagcgagg gattgactca ggtatttaat
     1801 gacgtacaca aatacaaccc caaaaccaat agctgggtta aattgatgtc gctcgcgccg
     1861 atgggcatgg cgggtcatgt gacttttgta cacaacggca aggcttatgt tactggcggt
     1921 gttaaccaga atatcttcaa tggctatttt gaagatctca acgaggctgg aaaagattca
     1981 accgctatag ataaaatcaa cgcccactat tttgacaaaa aagcagaaga ttatttcttc
     2041 aataagtttc tgttgtcttt tgatccctca acacagcaat ggagttacgc tggcgaatcg
     2101 ccctggtacg gaacggctgg tgcggcggtt gtgaataaag gtgataaaac ctggcttatt
     2161 aatggcgaag ccaaaccagg attgcgaacg gatgccgtat ttgaacttga tttcaccggt
     2221 aataatttaa aatggaataa gcttgatccc gtctcatcac cagatggcgt cgctggcggt
     2281 tttgcgggga taagcaatga ttctcttata tttgccggag gggccggatt caaaggttca
     2341 cgagaaaatt accagaacgg taagaactat gcgcatgaag gcctgaaaaa atcatatagc
     2401 actgatattc atctttggca taacgggaaa tgggataaat cgggtgaatt atcgcaaggt
     2461 cgggcctacg gagtatcatt gccctggaat aatagtttat taattattgg cggtgaaact
     2521 gcaggcggca aagcggtgac ggattcagtt ttaatttctg tgaaggataa taaagtcaca
     2581 gtacaaaact aacgcttcag ggccccggta aaggggccct gctatcaatt tgtcatttgt
     2641 attacgcgaa aattatatga acgcaataat atcgcccgat tattactatg ttcttaccgt
     2701 tgctggtcag tctaatgcca tggcgtatgg cgaaggactg ccattaccgg acagggaaga
     2761 tgcgcctcat tccagaatta aacaattagc gagatttgcg catacgcatc ccggaggccc
     2821 gccatgtcac tttaacgaca ttattccact gactcactgc ccacacgatg ttcaggatat
     2881 gcagggttat caccatcctc tggcaacgaa tcatcaaaca cagtacggca ccgttggcca
     2941 ggcactgcat attgcacgga aattactgcc cttcattcct aataatgcag ggattcttat
     3001 cgttccgtgt tgccgtggcg gatcggcttt taccgcgggc agcgaaggga catattcaga
     3061 acggcacgga gccagccatg atgcttgtcg ttggggaacg gatactccgc tgtaccagga
     3121 tttagtcagc agaacgcgag ccgcactggc aaaaaatccg cagaacaaat tcctcggcgt
     3181 atgctggatg caaggcgaat ttgacttaat gaccag
//
LOCUS       DAJTYZ010000257         3202 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000257 DAJTYZ010000000
VERSION     DAJTYZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.262"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1125
                     /locus_tag="OO596_004168"
     CDS             <1..1125
                     /locus_tag="OO596_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HCR6719841.1"
                     /translation="GLTGTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSF
                     ILVLTGPFMIYVYEPLLQSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEY
                     GTARAWGSFGYAIGAFFAGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAAD
                     AGGVKKEDFIAVFKDRNFWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLY
                     GYLNSFQVVLEALCMAIIPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVK
                     LLHAIEVPLCVISVFKYSVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAG
                     YQTVFFAISGIVCLMLLFGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1654..1854)
                     /gene="torI"
                     /locus_tag="OO596_004169"
     CDS             complement(1654..1854)
                     /gene="torI"
                     /locus_tag="OO596_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCR6719842.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(1977..2321)
                     /locus_tag="OO596_004170"
     CDS             complement(1977..2321)
                     /locus_tag="OO596_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719843.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2563..3165
                     /locus_tag="OO596_004171"
     CDS             2563..3165
                     /locus_tag="OO596_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719844.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          792 a          650 c          740 g         1020 t
ORIGIN      
        1 gggttgacag ggacggaatt aggtacactt tattcggtca accagtttac cagcattcta
       61 tttatgatgt tctacggcat cgttcaggat aaactcggtc tgaagaaacc gctcatctgg
      121 tgtatgagtt tcatcctggt cttgaccgga ccgtttatga tttacgttta tgaaccgtta
      181 ctgcaaagca atttttctgt aggtctaatt ctgggagcgc tcttttttgg cctggggtat
      241 ctggcgggat gcggtttgct tgacagcttc accgaaaaaa tggcgcgaaa ttttcatttc
      301 gaatatggaa cagcgcgcgc ctggggatct tttggctatg ctattggcgc gttctttgcc
      361 ggcatatttt ttagtatcag tccccatatc aacttctggt tggtctcgct atttggcgct
      421 gtatttatga tgatcaacat gtgttttaaa gataaggatc accagtgcgt agcggcggat
      481 gcgggagggg taaaaaaaga ggattttatc gcagttttca aggatcgaaa cttctgggtt
      541 ttcgtcatat ttattgtggg gacgtggtct ttctataaca tttttgatca acaacttttt
      601 cctgtctttt atgcaggttt attcgaatca cacgatgtag gaacgcgcct gtatggttat
      661 ctcaactcat tccaggtggt actcgaagcg ctatgcatgg cgattattcc tttctttgtg
      721 aatcgggtag ggccaaaaaa tgcattactt atcggtgttg tgattatggc gttgcgtatc
      781 ctttcctgcg cgctgttcgt taacccctgg attatttcat tagtgaagct gttacatgcc
      841 attgaggttc cactttgtgt catatccgtc ttcaaataca gcgtggcaaa ttttgataag
      901 cgcctgtcgt cgacgatctt tctgattggt tttcaaattg ccagttcgct tgggattgtg
      961 ctgctttcaa cgccgactgg gatactcttt gaccacgcag gctaccagac agttttcttc
     1021 gcaatttcgg gtattgtctg cctaatgttg ctatttggca ttttcttcct gagtaaaaaa
     1081 cgcgagcaaa tagttatgga aacgcctgta ccttcagcaa tatagacgta aacttttccg
     1141 gttgttgtcg atatctccct atccctcaac cggaaaataa taatactaaa gtgcttagcc
     1201 cggctaataa tcacctaatc caaacgcctc attcatgttc tggtacagtc gctcaaatgt
     1261 acttcagatg cgcggttcgc tgatttccag gacattgtcg tcattcagcg acctgtcccg
     1321 tgtatcacgg tcctgcgaat tcatcaagga atgcattgcg gagtgaagta tcgagtcacg
     1381 ccatatttcg ctatcaggat tctgtgtgat ggttacatcg cccggcccag ggctgtttag
     1441 tcatcagcgc tttctgacag tgctgagatt tcaacctgtt gcagtaaaaa tgagtagata
     1501 taaggcaagt gtgctgccaa acccatcttt tacggggtga aggtagattt cgtttgaagg
     1561 gtatctggtg tcccctgcag acatctactt gaggcggcag gggattgatt ggaatgctgt
     1621 tttttagatt tgaaaaatat tttacccgct attttaccca ttggcgcggc ttaagagctt
     1681 atttttgaat tcacaatggt cacgatataa ccatcttgct cgtccgtgga taactttggc
     1741 tttaggcagg tcgccggact taatccggtc atagatgaag gtcttaccga agccagtatc
     1801 agccatgatg aatttcaaat caaccagtga atcaggttgt agttcgtgtt gcatgagtgc
     1861 tatctccgaa tatggaatcg aacctgcaaa tcaggcaata aaaagccgca ttgatgcagc
     1921 aatggtaggt ctggatatct tgagaaatga acaggcctca tcgagtgtga ggcgggttag
     1981 tccttgcgta gctcgctgat tcttctgtaa gtctctggtg ctttgttccc gtacgtcttc
     2041 atttcagact tcaacagagc aacgagtgaa tcccattcgt tgaggattcc tttgaatgcc
     2101 ggaacgcgct ttgcaacctt gtcgaatgaa tctctgattt ctggaatctg ctcaacaagt
     2161 gcaacgcatc gccgaaagtc tgctgcgtca tggagagcgc cgaagtgatg accatagata
     2221 ttctttttca ggccacatgc gattgaggca agagttgcgc tactgatgcc gacatcgcca
     2281 gttgattgcc atttcaaaac cttcatagcc aaatctgaca tttcttgtct ccataaaaca
     2341 aaactcgccg tagcgagctc agataaaaga aatccccgtc agtgcgagga tgctgttcat
     2401 tgctgctata cacttttttg ctctcaacgt aagcggtagc ccattctgtt gggttggtca
     2461 gttgctttta ggaaatgctc tttacccctt aaacgtcggc tgaaagagct aaaatccatg
     2521 caaaaaattt acgcaatttt gtgtattatt gtgcagtaag taatgagcta ttttctgtgc
     2581 aaaaaatgga tgataaattt gtccggggca ggaaaaattt tatgggcgct aaacatgaaa
     2641 aaagattcgt atccttattt gatttgcatg acagtttcag ggctgatctt tattttcctt
     2701 ttcttctggt ggcgggcaga tatctacagg gtcacgtttc ttaatcagag tatatcccac
     2761 tattacattc tgtttagcat gggaatagct tttctgttat ctctgttttg ggttaagaag
     2821 gggatagtaa aacaaagcgg ctggaagagt ctgtcagcat accttaaggt ttatgcaggg
     2881 atatgcatat tttctggatt ttttctgatt atacccctta caacactaac ttattttttg
     2941 cctggagaga catcgtctta tgttgcaccg tatcggtata cttccggtag ttcaaaaagt
     3001 tgttctggag ctgaggtgga tgaccccgat ctacatgaga atattcgcat ttgctatccg
     3061 tatggcaatt atgagtacga taatattatc tatgttgaaa agaaaattaa tacattaggt
     3121 gcggtagtga catatgcaca gaccgcgcgt gatgatactg aatgagatag tatatagcgg
     3181 gcaagtttta gttaatttat cg
//
LOCUS       DAJTYZ010000258         3183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000258 DAJTYZ010000000
VERSION     DAJTYZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.263"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="OO596_004172"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="OO596_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6719845.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="OO596_004173"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="OO596_004173"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6719846.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="OO596_004174"
     CDS             complement(1715..1984)
                     /locus_tag="OO596_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6719847.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="OO596_004175"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="OO596_004175"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6719848.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="OO596_004176"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="OO596_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6719849.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          771 c          769 g          811 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gca
//
LOCUS       DAJTYZ010000259         3175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000259 DAJTYZ010000000
VERSION     DAJTYZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.264"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..936
                     /locus_tag="OO596_004177"
     CDS             <1..936
                     /locus_tag="OO596_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCR6719850.1"
                     /translation="KLEESSVFLDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTV
                     QTAFKMVNSAVSAGARLLQGDLSVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQ
                     SITTMSGITLDPGLAPRAVWPTDSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQ
                     PGSLVKNQQAVVAVGGSTERQSDIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRK
                     SLNAAIEQELRRTADDRLFFQLTSLRTELNRDIQARLVQTEETAERTPAEVLPALVLA
                     ASWYDDASRETDILDRNAISHPGFVPVRALRVPVR"
     gene            933..2015
                     /locus_tag="OO596_004178"
     CDS             933..2015
                     /locus_tag="OO596_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR6719851.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLGENILSCDTERSIRERFSEYQVSGQRPGND
                     DDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETT
                     YTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPAD
                     AYLPEPVTARKKKMLRRISDERVFSS"
     gene            1993..2526
                     /locus_tag="OO596_004179"
     CDS             1993..2526
                     /locus_tag="OO596_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR6719852.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            2532..2942
                     /locus_tag="OO596_004180"
     CDS             2532..2942
                     /locus_tag="OO596_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6719853.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            2935..>3175
                     /locus_tag="OO596_004181"
     CDS             2935..>3175
                     /locus_tag="OO596_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCR6719854.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKR"
BASE COUNT          721 a          775 c          937 g          742 t
ORIGIN      
        1 aaactggagg agtcgtccgt attcctggat gagttgattg aagacatgtt cggcgatttt
       61 gatctcgcgg gaataccgga ctttattcag aacgatgtta ttgcccggac gaccgatatg
      121 ctgggaaccg ttcagacagc tttcaaaatg gttaattcgg ctgtttctgc cggagcgaga
      181 ctactgcagg gcgatttatc cgtcattctg atgccgccgt cggttgccag tgattttgtg
      241 catacgttgc aggatacctg gcgggccgga acccggctgg tggataacac acaggatctg
      301 gtgcagtcca taacgacaat gtccggcatt acgctggacc caggactggc accgcgtgcg
      361 gtgtggccca cagattccgc atcggttatc aggcaaaaac agcagacaaa ccttgtggct
      421 gctgtcatcc ggacgacggc aatcagcgaa gccacaaggg cggtcacttc actgccacaa
      481 cccggaagcc tggtgaaaaa tcagcaggcg gttgtggctg ttggtggttc cactgaacgt
      541 cagtccgata ttattcacgt ttctcatccg gcacttgaca gcgtggcagc cagcacagga
      601 caggatgaga gagcgcaacc acccacgcgg gaaaatctca ccatcatacg caaatcgctg
      661 aacgcggcga ttgaacagga gctcaggcgt acggcggatg acaggctgtt ttttcagctg
      721 acgtcattac gtacagaact gaaccgggat attcaggcgc gtctggttca gacggaggaa
      781 accgcagagc gaacgccagc ggaagttctg cctgcgcttg ttctggctgc gtcatggtac
      841 gacgatgcgt cccgtgaaac tgatatcctg gatcgaaatg ccatctctca tccgggcttt
      901 gttccggtca gggcattaag ggtacccgtc agatgaatga taccgtttta cttcgggttt
      961 ccggtcgcga atggggcggc tggacatccg tccgtatcag tgcgggcatt aaccgtattg
     1021 cccgggattt taatgttgcc attaccaccc gctggcccgg aagccgggat tatcaacccc
     1081 ggataaaaaa tggtgagctg gttgaagtgc ttatcgggga tgagcctgtg ctcaccggat
     1141 atgtggaggc actaccgctt cgttatgacg ccagcagcgt cagcatggga attgtcgggc
     1201 gaagcaaaac agccgatctg gttgactgct ctgctttgcc actccagcag agcggaaaaa
     1261 acctgctcag aatagtcagt gaactggctg cgccatttgg catcaccgtt gttgatgctg
     1321 gcgtgccgca gacagcggtg attgatgccc agccggaaca cggcgaaacc gttgccgatt
     1381 gtcttaaccg gttactgggg caggttcaga cgctggctta tgacgatgaa tgcgggcgac
     1441 tggttctggg aaaacccgga acaggtaaag cggcgacggc gctggtgctg gtgctgggag
     1501 agaatattct ttcctgtgac acggaaagaa gcatcagaga gcggttttct gaatatcagg
     1561 tcagtgggca gcgcccgggc aacgacgatg attttggtga ggccaccatt gccgcaatac
     1621 gtcagaccat tcaggacagt ggcgtgaccc gttatcgccc tttgttgatt cagcagtcag
     1681 gcacagcaac gacagcaacc tgtaaggccc gttgtgaatt tgaagcgcgc caacgggctg
     1741 cgcttacccg tgagacaaca tatacggttc agggctggcg gcagggcagt ggcgcgttat
     1801 ggcgtccggg gttatctgtc atcgttttcg acccgctgaa taattttgat aatgatgaac
     1861 tggtgatcgc agaagttacc tataaccagg acgaccgggg cacgacgact gaattacggg
     1921 ttggcccggc agatgcttat ctccccgagc ctgttaccgc caggaagaaa aaaatgttga
     1981 ggaggatttc tgatgaacgg gttttctctt cgtaatctga tttcccgggc tgtcatcacg
     2041 gcggtggatt ccgccagaaa gtgtcagtct gtagggttga aaatgatagc cggagatcag
     2101 aaacaacacg ttgagcacct tgaaccttat ggttttacat ctgccgcaca gaacggtgct
     2161 gagggcgttg ctttatttcc ggcgggcgat cgttctcatg gtgtggttgt ggtcgtggct
     2221 gacagacgtt accggctgaa aggactgaaa cgtggggaag tggcgcttta tgacgatctg
     2281 ggacagtccg ttgtcctgac ccgttccggt attgtggtgg acggggccgg gaaggccata
     2341 atttttaaaa acgcgcctaa agcgcgcttt gaaatgccag tcgaatccac cgccgatatt
     2401 actgacaatt gcgacagtgg cggactcagc atgcagcaaa tgcggcaggc ctacaatgcc
     2461 cacaaacata ccgaaaatgg tgatggtggc gggatcactg acacgccgga tcaaccgatg
     2521 ggctgaaaat catgatgatt aatgttaacg ggcgacccgt gtcgaccggg gcttcgatcg
     2581 accttctgac gcgtgctgtg attatttcgc tttttacatg gcggcgtgcc gggcgtgatg
     2641 atgcaccacg gatatttgga tggtgggggg atacctggcc tgcggttcag aatgatcgca
     2701 cggggtcgcg tttgtatctg ttgcgacgca gcaagctgac aaataaaacc ccgcagcttg
     2761 ccagagatta tgtccgtgag gcgctggcgt ggatggtgga ggatggtgtt gcttcccgtc
     2821 ttgatattaa cgctgtccgg accgggacag actcgctggc acttgccatt accatttacc
     2881 agcgtgacgg caatattcac aacattattt ttgatgatat ctggagtgaa ctgaatggct
     2941 gacagtcagt tttatcgccc cggcctcccg caacttattt ctatgatccg gagcgattta
     3001 ttaacccgct ttgagcagga tacgctgctt cgtcgtatgg atgcggaagt gtatgcccgt
     3061 gtacaggctg cagccgtaca cacgttgtac gggtatatcg attatcttgc cagaaatctg
     3121 ttaccggacg tgtgtgatga agactggctg taccggcacg ccagaatcaa acgct
//
LOCUS       DAJTYZ010000260         3143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000260 DAJTYZ010000000
VERSION     DAJTYZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.265"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..401)
                     /gene="ydcO"
                     /locus_tag="OO596_004182"
     CDS             complement(<1..401)
                     /gene="ydcO"
                     /locus_tag="OO596_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCR6719855.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIP"
     gene            454..990
                     /gene="sutR"
                     /locus_tag="OO596_004183"
     CDS             454..990
                     /gene="sutR"
                     /locus_tag="OO596_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCR6719856.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1063..3024
                     /gene="rlhA"
                     /locus_tag="OO596_004184"
     CDS             1063..3024
                     /gene="rlhA"
                     /locus_tag="OO596_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR6719857.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
BASE COUNT          798 a          780 c          819 g          746 t
ORIGIN      
        1 ggaataatgc gcatcagacg agcaaagagt cccgttatgc cgcagaggac tattaacgcg
       61 ttggtgacaa taaaaacgcc gatggcttcg ttaagtgtta gtccctgcaa tccggtgacc
      121 aacaaagccg cgccaggcgt tgaccatgcg gtgagaacag gtacgcgata ccataatgtc
      181 agagtcagcg tactgacgcc cattgccagc cccagcgccg tcatccagcc agagatttgt
      241 gcagtggtgg ctccggcgac aatcgctgct tgccagatta ttgccgctga actggcgtag
      301 ccaattaata ccgccagaaa ccccgccagt agcgtgggtg gaggaataga aaacagacgc
      361 atagtcacct cgtgcgttat agcgtacagg caccgtagca tttgtccgtt ataacgcaca
      421 agtgataaac ttccgttttg ccggaggagt cgcatggaaa atctcgctcg ctttttatcc
      481 actacactta aacaactacg ccagcagcgc ggctggagtc tttcgcgact ggcagaagcg
      541 acgggcgttt ctaaagcaat gttagggcag attgagcgta atgagtccag tccgacggtc
      601 gcgacattat ggaaaattgc caccgggttg aacgtaccat tttcaacatt tatttctccg
      661 ccgcagtccg ctatgccttc agtttatgat ccacaacagc aggcgatggt gatcacttcg
      721 ctgtttcctt atgatccaca actctgcttt gaacacttct cgattcagat ggcatctggt
      781 gcaataagcg aatcgacgcc ccatgagaag ggggtaatcg aacatgtcgt cgtcattgat
      841 ggacaacttg atctgtgcgt tgatggggaa tggcagaccc ttaattgtgg ggaaggcgtt
      901 cgatttgctg cagacgtcac gcatatctat cgtaatggtg gggagcaaac cgtacatttt
      961 cattccctca tccattaccc gcgcagttaa gcgggaaaac tatttcgcaa cgtcgcgctt
     1021 gtggctaaaa tagccgccat ttttcagcta ctggataaga atgtgaccgt atcttctcat
     1081 cgacttgaac tgttaagccc ggcacgcgat gccgccattg cccgcgaagc tattttgcac
     1141 ggtgccgatg ctgtttatat cggcggccct ggttttggtg cccgtcataa tgccagtaat
     1201 agcctgaaag atattgccga gctggtgccg tttgcccatc gttatggtgc aaaaattttc
     1261 gtcacgctta acaccatttt gcatgatgat gagctggaac ccgcgcaacg gctgattact
     1321 gacctctacc agaccggtgt cgatgcgctg attgttcagg atatggggat tctggaactt
     1381 gatattccgc cgattgaact gcacgccagt acgcagtgcg acattcgtac agttgaaaaa
     1441 gcgaagttcc tctctgatgt tggcttcacg cagattgtgc tggcgcgaga gctgaatctt
     1501 gatcagatcc gcgcgattca ccaggctacg gacgcgacca ttgaattctt tattcatggg
     1561 gcactgtgcg tggcctattc gggtcagtgc tacatttctc atgcgcaaac agggcgtagc
     1621 gccaaccgtg gcgattgctc gcaggcgtgc cgtttgccat acacattgaa agacgatcag
     1681 gggcgggtgg tttcctatga aaaacatctg ctgtcgatga aagataacga tcagactgcc
     1741 aacctcggcg cgctgattga tgctggcgta cgctccttca agattgaagg gcgttacaaa
     1801 gatatgagct acgtgaagaa tatcaccgcc cattatcgcc agatgctgga tgccattatt
     1861 gaagaacgtg gcgatctgac gcgtgcttca tcaggtcgta ctgaacattt ctttgttcca
     1921 tcgacggaaa agactttcca ccgtggtagc acagattatt ttgtgaatgc ccgtaaaggc
     1981 gatattggcg cgttcgattc gccgaaattt atcggcctgc cggtgggtga agtattgaaa
     2041 gtggcgaaag atcatctcaa tgttgccgtc accgagccac tggcaaatgg tgatggcctg
     2101 aacgtgatga ttaaacgtga agtcatcggt tttcgtgcca atacggtcga gaaaaccgga
     2161 gaaaatcagt accgcgtctg gcccaatgaa atgccagcag atttgcacaa aattcgtcca
     2221 catcacccac taaaccgtaa tcttgatcat aactggcagc aggcactgac aaaaacctcc
     2281 agcgaacgtc gggtggcggt agacattgaa ctgggcggct ggcaggaaca actgattctg
     2341 accctcacca gtgaagaggg tgtcagcatc acgcatacgc tggacgggca gttcgacgaa
     2401 gccaataacg ccgaaaaagc aatgaacaat ctgaaggatg gtctggcaaa actggggcaa
     2461 accctctatt acgcccgcga tgtgcaaatt aatttgccgg gggcgctgtt tgtaccaaac
     2521 agtctgttaa accagttccg ccgtgaagct gctgacatgc tggatgctgc gcgtcttgcc
     2581 agttaccagc gcggcagccg taaaccggtt gctgatcctg cgccggtttt tccgcaaacg
     2641 catctgagtt tcctcgcgaa cgtatacaac cagaaagcgc gtgaatttta tcatcgctat
     2701 ggtgtgcagc tgattgacgc ggcgtatgag gcacatgaag agaagggcga agtcccggtg
     2761 atgatcacca agcattgtct gcgctttgcc tttaatctgt gcccgaaaca ggcgaaaggc
     2821 aatatcaaaa gctggaaggc gacgccaatg caactggtta acggcgatga agtattaacg
     2881 ctaaagtttg attgccgccc atgcgagatg cacgtcattg gcaaaatcaa aaatcacata
     2941 ctgaaaatgc cgttaccggg aagcgtagtg gcatccgtaa gtccggatga gctgctgaaa
     3001 acattgccta agcgaaaagg gtaaaacgcc agttttctgg ttactcacaa ctcattgaat
     3061 ctgcatgata ttgcctgccg ggtaaggcgt cacgccgcat ccggcatcaa caaagcgcac
     3121 gttgttatcc ggttatcaag cca
//
LOCUS       DAJTYZ010000261         3142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000261 DAJTYZ010000000
VERSION     DAJTYZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.266"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            61..576
                     /locus_tag="OO596_004185"
                     /pseudo
     CDS             61..576
                     /locus_tag="OO596_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            574..705
                     /locus_tag="OO596_004186"
                     /pseudo
     CDS             574..705
                     /locus_tag="OO596_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013183226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(799..1272)
                     /locus_tag="OO596_004187"
                     /pseudo
     CDS             complement(799..1272)
                     /locus_tag="OO596_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1445..2227
                     /locus_tag="OO596_004188"
                     /pseudo
     CDS             1445..2227
                     /locus_tag="OO596_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            2805..>3142
                     /locus_tag="OO596_004189"
     CDS             2805..>3142
                     /locus_tag="OO596_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6719858.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLT"
BASE COUNT          668 a          892 c          841 g          741 t
ORIGIN      
        1 ggcacggaac cacggcagct tgacggctgc aatgacgtgt gtcgtctggc ggtggagtat
       61 ctgctgtatg cagcccgaaa acgggggctg gaaatcggca tcttctgcac catccacact
      121 ttacgccttc actttgaaga acatttaccc ctcgttgtag ccggacgtcg cctgggcgta
      181 ccaaaatcaa cagtttgtag tatgtttgtg cgcttccgca aagctggcct ttcatggcct
      241 ctgcccgcag gtatgtcgga gcgggaactt gacgcccgtc tttacgggag tgcctccaca
      301 gtacctgtcg tacttactga aagtaccgtt atgcccgaag tccccggggt aaaaaagcgt
      361 ccccggcgcc ccaacttccc ttatgagttc aaaatcgcct tagtggagca gtcactgcag
      421 cccggagcct gtgtggcgca gattgcccgt gaaaatggaa tcaacgataa cctgctcttc
      481 aactggcgcc atcaataccg gaaaggtggc ctgctgcctt ccggaaaaaa tatgccggca
      541 ctgcttcccg tgacgttaac gccggagccg gataaccatg gttttgacat catctatatg
      601 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgatccata cctgatcggc
      661 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      721 gtggtttggt gcctgcgcct gcacgccgac accgaggggc cttccctcat ctcttgtgca
      781 gcatggctgc acagggactc acgtccctgg tgcgcgcctt cgtggcacac catcatcggc
      841 gtacctttcg aagggaacgt cgggacactg acgttgcatc cacatatcaa aggcatagtg
      901 caggaacaga tttgagagca ggggcgagat cacgccgccc tgcggcgttc cttctgtcac
      961 cggccttacc agacctgcgt ccattacccc tgcattcagg aacctgcgga taagtgacaa
     1021 cacccgttta tccgatactc gtttctctat ccggctcatc agcacgtcgt gatttatccg
     1081 gtcgaagaac ttctccagat cgaggtcgac cacccagtga tacccggccc cgatatattc
     1141 ctgggcctgt atcactgcct ggtgggccga acgaccggga cggaacccgt agctgttgtc
     1201 gctgaacgag ctatctcaca gtgcctgcaa tacctgcatc atagcctgct ggatgaagcg
     1261 atccaccacc atctggatgc ccagcagacg ttcgccgccg ccgggcttca gtatggatac
     1321 tcttctcaca ggggatgggc ggtatctgcc ggacagcagt tgcgctttca gctctggcca
     1381 gtggtgcctc aggtagtccg gcagttcact tacgcgcatg ccatcgactc ccgccgcacc
     1441 ttgattgcgt cttgtcgatg gaacagcaat cagcgcgccc gggggcggca gcgctgaatg
     1501 gcgactacat atgggatatg atcctcatac ctgtcagttc actgattttg agctaaccga
     1561 cagcagagac gctgaacggc tggatcgatt tgcgcaaacg gcagacgaga tacgaattgc
     1621 tgaccgggga ttcggttcgc gtcccgaatg tatccgctca cttgcttttg gagaagctga
     1681 ttatatcgtc cgggtttact ggcgaggatt gcgctggtta actgcagaag gaatgcgctt
     1741 tgacatgatg gattttctgc gcgggctgga ttgcggtaag aacggtgaaa ccactgtaat
     1801 gataggcaat tcaggtaata aaaaagccgg agctcccttt cctgcacgtc tcattgccgt
     1861 atcacttcct cccgaaaaag cattaatcag taaaacccga ctgctcagcg agaatcgtcg
     1921 aaaaggacga gtagttcagg cggaaacgct ggaagcagcg ggccatgtgc tattgctaac
     1981 atcattatcg gaagatgaat attcagcaga gcaagtggct gattgttacc gtctgcgatg
     2041 gcaaattgaa ctggctttta agcggctcaa aagtttgctg cacttggatg ctttgcgtgc
     2101 aaaggaactt gaactcgcga aagcgtggat atttgctaat ctactcgccg catttttaat
     2161 tgacgacata atccagccat cgctggattt tccccccaga agtgccggat ccgaaaagaa
     2221 gaactaacgc gttgtggaga ataacaaaaa tggtcatctg gagcttacag gtggccattc
     2281 gtgggacagt atccctgaca gcctacaaaa cgcaattgaa gaacgcgagg catcgtctta
     2341 acgaggcacc gaggcgtcgc gttcttcaga tggttcaacc cttaagttag cgcttatggg
     2401 gtaaatcccc gcctttttcc tgccggtccc cggacagagc acaaaggaag tcgccgccgg
     2461 ctccgcttta cccggccatg cggggcatgg ccttgtgggt tttcagctct gtagcctcag
     2521 cgtcgtgtgc gggctgtgcc gtgcctccat cttagccggg ctggcgttga tgcaagggta
     2581 cgcttcgccg ctgcggtcac ccggtccctc cttcccgtct gccgtgattt tccggtcgtt
     2641 cacccgctca gatttcgcgg tttcaccctc caactcccgc cagccgtgtg cgtcaggctg
     2701 cggcttccct tgcgccctgt gcatccccgc cttatcgccc ggctaagatg gaggcacggc
     2761 accgcccggt gtcgcggaat atgtgaacaa cggaggaaaa catcatgcaa tatgcgaaac
     2821 ctgtcactct gaatgttgaa gagtgcgacc gtctctcctt tctgccttac ctgtttggca
     2881 acgattttct gtatgccgag gcgtatgtgt acgcgctggc gcaaaaaatg atgccggaat
     2941 atcagggcgg cttctggcac ttcatccgcc tgccggacgg tggcggttac atgatgccgg
     3001 atggcgaccg tttccacatg gtgaacggtg caaactggtt tgaccgtacc gtgagtgctg
     3061 atgccgcagg catcattctg acttctcttg tgattaaccg ccagttgtgg ctgtaccacg
     3121 acagcgggga tgcaggactg ac
//
LOCUS       DAJTYZ010000262         3081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000262 DAJTYZ010000000
VERSION     DAJTYZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.267"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..309)
                     /locus_tag="OO596_004190"
     CDS             complement(<1..309)
                     /locus_tag="OO596_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCR6719859.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     R"
     gene            complement(306..692)
                     /gene="trbI"
                     /locus_tag="OO596_004191"
     CDS             complement(306..692)
                     /gene="trbI"
                     /locus_tag="OO596_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCR6719860.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(689..1939)
                     /locus_tag="OO596_004192"
                     /pseudo
     CDS             complement(689..1939)
                     /locus_tag="OO596_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(1941..2123)
                     /locus_tag="OO596_004193"
                     /pseudo
     CDS             complement(1941..2123)
                     /locus_tag="OO596_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2145..2456)
                     /gene="traL"
                     /locus_tag="OO596_004194"
     CDS             complement(2145..2456)
                     /gene="traL"
                     /locus_tag="OO596_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCR6719861.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(2471..2821)
                     /gene="traA"
                     /locus_tag="OO596_004195"
                     /pseudo
     CDS             complement(2471..2821)
                     /gene="traA"
                     /locus_tag="OO596_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(2867..>3081)
                     /locus_tag="OO596_004196"
     CDS             complement(2867..>3081)
                     /locus_tag="OO596_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6719862.1"
                     /translation="NAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNNRRLL
                     ERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          708 a          884 c          754 g          735 t
ORIGIN      
        1 ccgcccttcg ttatcccgga tatccgcagc cagtctgacg gacgggtcaa acaaccggct
       61 gctgtatttc tccgtgcgtc cgataccggg cacggcaggg ggccgcagac tgttgcggat
      121 cacccgctcc ttaaaggcat ccattttccg gcccatctca ccggactgct ccagtgccgt
      181 caggcgctgc atgatcaccg tcagcatgtc cggctccttt accggccaca gatcacccca
      241 ggtgccaaga tcggcggcgg ccacactctg cccccatatc agcagggcaa tcagcccccg
      301 gcatctcatg gttccgctct cattcgccgg gcaatatcct gctggatttc ccgggtgata
      361 tccggagccc cctgtaccac ggcaggcgac accagaatga ccgcgtggtg tttctgctgc
      421 cacgcctgca ggctggcttc cagtgccgtg ttaaaacgcg ctgaaagggc ttttgactgt
      481 gcttctgaca gttgtttctg actggcgctg tcaaaaaatg catccacagt ctgtttcatg
      541 ttgaaggcgg cagtcatcgg tgcgttcagt ctgacaatgc cgtaactgac ggctgcgttc
      601 agtaacacca ttgccaggca tcccggcacc gtccaccacc agtggctgcg tcggggggct
      661 gtaacatcag tcgttttttg cgttgtggtc atgcaacact cctgtatttc tcatgttctt
      721 ccagccaggc ttccagcgaa gccatctccg gccctgactt cttccacgcc agctgccaca
      781 ccgcctcatg aatactcagc ccctctttac gtttctgctg cacaaactca aaatccgggc
      841 cgtcagagct gtacatggcg cggcttaacg ggtccacaaa caggcggtgc caggaggaat
      901 ggttttccac ctgcagcagg aaggaactga accactggtc tttggccgca ccaaacttac
      961 caatcatgtc gcgctgcagt ggctggaact ggtccggata cagctggttg tattttgcga
     1021 actccttcgc actctgtttg aggataattt tgtaggagga gttaccccat gccgcacggg
     1081 ccgcactgga ggccttgtca gagtcaaagt cgacgatgtt ctgtgtgatg gtgatataag
     1141 caccggtatg acgacgggcg gtacggtaac ctttctcaat aaactcaccg accttgtgat
     1201 ttttgaagtc cagcagacgc cagccttcat caatgacgtt cagtttcttg aggttacgcg
     1261 gcgtgcggta catcctgttc tcgatgtaga taatcaggga gaacatcacc gcaaccagca
     1321 gtgacggacg gtcttccagt ccgcccagct ccagcaccac catttttgcg tcatcccgca
     1381 gggacggctc atcagagtta aaatactggc cgtaagtgcc gttggcagtg tactggtcaa
     1441 gcagcacaat catttcgtcc agacggctgc ggatagtcgg tgacccggca tactgctcgc
     1501 tgtcactggc gtttttcagg aaatccacca cgtcatcaat acgtgccctg ttctctttgg
     1561 ccagccagga ggccctgacc gcctgcagca gcagaccttc atgcacttca tccaggttac
     1621 cgttggggct ggccatcact gacaactggt cacggacacg ctccgctgac tggtcgatat
     1681 cggtgatgtt cgcaaacggg ttaaaacgca gggtttcacc gtccagatat acgccaccca
     1741 tgttctcaca cagggacttg tagccatccc ccatgtcgaa caccacggca aagcccccgg
     1801 agtccagcac gctgcggata agtggctgta tcagcccggt tttaccggcc ccggaggtgc
     1861 cacagaccgc catgttgtag ttggtgttat tcattccccg gaagaaaata tcgataaacg
     1921 ccagctggtt acggtaggtg tgacacggca aagggcacat taaatcccat tggggtcacg
     1981 gcagtgcgtt gctcattctg aagacggtag ttattcaccc cctgaatgag gttaacagag
     2041 aggctgagaa caataagcac tgacataaat ataaaggcga tggccattac acgactggta
     2101 cttaaacggg caccgtgttc catataacaa tcctggtatc agttctattt aatccactgc
     2161 cggaaacacg aatcgggaac attatgaaaa ataccgcgca gcagggctgt tggcatatac
     2221 cagtaaatca ggtcacgtaa ccaggaactg ccccgccctt ttttcagttt tttaatcccg
     2281 aaataaacca gaaccgctgc accaataccg aacagatatt tcgatgttgt gataccccag
     2341 ccaacacaga ttgctgcggg gatcagttca tccagtggca ggccaaacca gcggctctgg
     2401 ttggtcagtg tttccgggaa acgatatttc ttaagtttat tctcgtctcc caacatcgtt
     2461 ttatttcctg tcagaatccg gcaacagaca tacctactgc aataaataca gagagtatgg
     2521 caaagccgac caggaacttg acgtttttgg tcatcatgta catgactgca ccgaccagaa
     2581 cttcagccag aacaacccat ttaacgatac tggagtcctt accgaaggtc gccttaaccg
     2641 tttcctgacc tttagccatc aggtccgtgc ctttaccagc agccatagct aactcaggga
     2701 acaacatcat cagaacagca accgggatca ctgcacgaac caggcgaagc atattcagac
     2761 gagtgaattt agaaaaaaac gatttctttt tgacgggcgc agaagtaccc tgaacactta
     2821 acgctgaacc gccccgggtt tcctggagag tgttttatct gtgaactcag gctgccagat
     2881 catcgtttcc gatggaagca taataagctt tttctgcttc tgccggagga gtatggccca
     2941 gcctttccag caatcgtcga ttgttatacc agtccaccca cgtgagtgtg gccagctcca
     3001 cttctgtccg gtttttccag ctcttacggt gtattacctc cgctttgtaa aggcccttga
     3061 tgatctctgc catcgcgttg t
//
LOCUS       DAJTYZ010000263         3047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000263 DAJTYZ010000000
VERSION     DAJTYZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.268"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..173
                     /locus_tag="OO596_004197"
     CDS             <1..173
                     /locus_tag="OO596_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719863.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            186..1037
                     /gene="hyi"
                     /locus_tag="OO596_004198"
     CDS             186..1037
                     /gene="hyi"
                     /locus_tag="OO596_004198"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6719864.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            956..2134
                     /gene="allB"
                     /locus_tag="OO596_004199"
                     /pseudo
     CDS             956..2134
                     /gene="allB"
                     /locus_tag="OO596_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2191..>3047
                     /locus_tag="OO596_004200"
     CDS             2191..>3047
                     /locus_tag="OO596_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCR6719865.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          696 a          711 c          836 g          804 t
ORIGIN      
        1 aggcgaaagc cttaatggcg caatatcggg taccggtagt cgtggaagtt attcttgagc
       61 gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt catggaattt gaagatatcg
      121 ccgataacgc agtggacgca ccgactgaaa cctgcttcat gcactatgaa taagggagat
      181 aaataatgtt acgtttctct gctaatttat cgatgttatt tggtgaatat gattttctcg
      241 cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt tgaatttatg tttccttatg
      301 actacgacat tgaagaatta aaacaggtgc tggcgagtaa taaactcgaa catacgctgc
      361 acaatctacc ggcgggtgac tgggcggcgg gggagcgcgg tattgcctgt attcctggcc
      421 gtgaagaaga gtttcgggat ggcgtagcag cagcgattcg ttatgcccgt gcgctgggga
      481 ataaaaaaat taactgtctg gtcggtaaaa cgccggctgg tttcagcagt gaacagattc
      541 acgcaacgct tgtagaaaac ctgcgttatg ccgcgaatat gctgatgaaa gaagatattt
      601 tattactgat tgaacctatt aaccattttg atattcctgg tttccatctc accggaactc
      661 ggcaggcgct gaaattgatt gatgatgttg gttgctgcaa tttgaaaatt cagtatgaca
      721 tttatcacat gcagcggatg gaaggtgaat taaccaacac catgactcag tgggccgata
      781 aaattggtca cctgcaaatt gctgataatc cgcatcgcgg cgaaccgggg accggagaaa
      841 ttaattatga ttatctcttt aaggtaatcg aaaattctga ctacaacggt tgggttgggt
      901 gtgaatataa accccaaacc accacggaag ccggtttacg ctggatggat ccgtactttc
      961 tgaaccgggt cgtagccact gggaaggtta tgaaaccggt actcgcgcag cggcaaaagg
     1021 tggtatcacc accatgatcg aaatgccgct caaccagctg cctgcaacgg ttgaccgcgc
     1081 ttcaattgaa ctgaagttcg atgccgctaa aggcaagctg actatcgatg cggcacaact
     1141 cggtggcctg gtgtcttaca acatcgaccg tctgcatgag ctggatgaag tgggcgttgt
     1201 cggcttcaaa tgcttcgttg cgacctgtgg cgatcgcggt atcgacaacg acttccgtga
     1261 tgtaaacgac tggcagttct tcaaaggtgc gcagaagctg ggcgaactgg ggcagccggt
     1321 gttggtgcac tgcgaaaacg cgctgatctg tgacgcaccg ggcgaagaag cgaaacgtga
     1381 aggtcgcgta actgcccatg actatgtggc ttcgcgtccg gtatttaccg aagtggaagc
     1441 gattcgccgc gtgctgtatc tggcgaaagt tgccggttgc cgtctgcacg tttgccatat
     1501 cagcagcccg gaaagtgttg aagaagtgac tcgtgcacgt caggaaggtc aggatgttac
     1561 ctgtgaatcc tgcccgcatt actttgtact ggataccgat cagttcgaag aaatcggtac
     1621 tctggcgaag tgttcaccgc cgatccgcga tctggaaaac cagaaaggca tgtgggaaaa
     1681 actgtttaac ggtgaaatcg actgcctggt ttccgaccac tcaccatgcc ctccggaaat
     1741 gaaagccggc aacatcatgg aagcatgggg cggtattgcc ggtctgcaaa actgtatgga
     1801 cgtgatgttc gatgaagcgg tacagaaacg cggaatgtct ctgccaatgt tcggcaaatt
     1861 aatggcgact aacgcagcag atattttcgg tctgcagcaa aaaggccgta tcgccccagg
     1921 aaaagatgcc gacttcgtct tcattcagcc gaatagcagc tatgttctta ccaatgacga
     1981 tctggaatat cgccacaaag tcagcccgta tgttggccgt accattggcg cgcgtatcac
     2041 gaaaaccatc ttacgtggtg atgtgattta cgacattgaa cagggcttcc ctgttgcgcc
     2101 gaaaggtcaa tttatcctta aacatcagca gtaatctggc ccctgcaatg cccgtccttg
     2161 cggcgggcat tctccggtta aggtgtgttt atgttcaatt ttgcagtcag ccgcgaaagc
     2221 ctgttatcag gatttcagtg gtttttcttt attttttgca acacggttgt ggttcctcct
     2281 acgctacttt ctgcttttca gttgccgcaa agtagcctgc ttacgctcac gcaatatgct
     2341 tttcttgcta ccgcactggc ctgcttcgct caggcgttct gcggtcatcg tcgcgctatt
     2401 atggaagggc cgggtggcct gtggtgggga accatcctta ctatcaccct tggcgaagca
     2461 tcgcgcggga caccgatcaa cgatatcgct accagtctgg cggtggggat tgcactctcc
     2521 ggcgtgctga cgatgttgat tggttttagc ggattaggcc atcgcctggc acggttattt
     2581 acgccgtcgg tgatggtctt gtttatgttg atgctgggcg cacagctgac cactatcttt
     2641 ttcaaaggta tgctcgggct gccgtttggc atagccgacc catattttaa aattcagtta
     2701 ccaccgttcg cgctctcggt ggcggtgatg tgcctggtac tggcgatgat tatctttctg
     2761 ccgcaacgtt ttgcccgtta tggcctgctg gtcggcacca taaccggctg gttgttgtgg
     2821 tacttttgct ttccttcttc gcattcgctc tccggtgagt tgcactggca gtggttcccg
     2881 ctcggcagtg gcggtgcttt gtcgccggga attattctga cggcggtgat tacaggtctg
     2941 gtaaatatca gcaataccta cggtgcgatt cggggcacgg atgtttttta tccgcagcag
     3001 ggcgcgggga atacgcgtta tcgtcgcagc tttgtggcga ccggatt
//
LOCUS       DAJTYZ010000264         2992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000264 DAJTYZ010000000
VERSION     DAJTYZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.269"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..1076
                     /locus_tag="OO596_004201"
     CDS             18..1076
                     /locus_tag="OO596_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR6719866.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="OO596_004202"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="OO596_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR6719867.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="OO596_004203"
     CDS             2805..>2992
                     /locus_tag="OO596_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719868.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAJTYZ010000265         2946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000265 DAJTYZ010000000
VERSION     DAJTYZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.270"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..797)
                     /gene="ycjG"
                     /locus_tag="OO596_004204"
     CDS             complement(<1..797)
                     /gene="ycjG"
                     /locus_tag="OO596_004204"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6719869.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARA"
     gene            916..1422
                     /gene="tpx"
                     /locus_tag="OO596_004205"
     CDS             916..1422
                     /gene="tpx"
                     /locus_tag="OO596_004205"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6719870.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(1466..>2946)
                     /gene="tyrR"
                     /locus_tag="OO596_004206"
     CDS             complement(1466..>2946)
                     /gene="tyrR"
                     /locus_tag="OO596_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6719871.1"
                     /translation="VLRGIDLRGIEIDPIGRIYLNFAELEFESFSSLMAEIRRIAGVT
                     DVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKVDMANPASCQLFGQKLDRLRNH
                     TAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLMEITPVYLQDENDQHVLTGAVVM
                     LRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVEQAQKLAMLSAPLLITGDTGTG
                     KDLFAYACHQASPRAGKPYLALNCASIPEDAVESELFGHAPEGKKGFFEQANGGSVLL
                     DEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVRVICVTQKNLVELVQKGMFRED
                     LYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQGVQRPKLAADLNTVLTRYAWP
                     GNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATVAVGEDAMEGSLDEITSRFERS
                     VLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQKKNEE"
BASE COUNT          698 a          842 c          684 g          722 t
ORIGIN      
        1 gcacgcgctt cagtcgccag cgccagcgct tccgtcagac caccgatttt atcgagctta
       61 atgttaacca tctcatagcg ccctttcagc gccttcagat tgctacgtgt atgacaactc
      121 tcatcagcac aaatcggcaa cggatgaata aaattctcca gcgccgcatc atcctgcgca
      181 ggaaggggtt gttcaagcat cgcaacgcct aaatccgcca atagctggca acgcgccgcc
      241 accccttctg cacgccagga ttcatttgca tcaacgatca gcgtcgcatc gggcacagct
      301 gtgcgaattg ccaccatccg ctcactaata agatggttat ccagcttcac tttcagtaat
      361 ttcgcgcctg cctgccagag tgttgatgca ctattggcca tctgatcagg cgtaccgatg
      421 actaccgtct gtgcagtgat aactgtctcg ggaagcgtta tgccgatcaa atcagccagc
      481 gattgctgct gttttcgcgc ggccagatcc cacaatgcac aatccagcgc attacgtgct
      541 gcgccggcag ggagaatttt ttgcaactcc tcccgtgtca gccctttctc taattgcgac
      601 acgacgctca taatttgcgc cattaccgag gcatcacttt ctccataacg cggatacggc
      661 gtgcattcgc cggtgccttt aataccctct tcttccagtt caaccaccac cacgcgcgct
      721 tcactgcgac ttccccgggc aatcacaaac ggggtatgta agggccaggc ttcctcaaat
      781 accttaacgg ttctcatctt tactccttgt tgtcgcggtg gtctgcggat taagcgcaaa
      841 tagcgtttgc tgtgttattg acagttagca taaactaggt gtgacgttaa ctatatgtaa
      901 acaggaaaga taatcatgtc acaaaccgtt catttccagg gcaacccggt tacagtcgcc
      961 aattccatcc cgcaggcggg tagcaaagcg cagactttta ctctcgtggc aaaagatctg
     1021 tctgacgtca ccctcggtca gtttgcgggt aaacgcaaag tgctgaacat tttcccgagt
     1081 attgataccg atgtttgcgc cgcatcagta cgcaagttta accaactggc aactgagatc
     1141 gacaacaccg ttgtgctgtg tatctctgcc gatctgccgt tcgcccagtc tcgtttctgc
     1201 ggcgcagaag gtctgaacaa cgttatcacc ctctccactt tccgtaacgc tgaatttctg
     1261 caagcttacg gtgtggcaat tgctgatggc ccactgaaag gtctggcagc gcgtgccgtt
     1321 gtggttattg acgaaaatga caatgtgatt ttcagccagc tggtggatga aatcaccacc
     1381 gagccggatt acgaagcagc tctggctgta ctgaaagcat aagttaatat gcctgatggt
     1441 gttgcaccat caggcatatt cgcgcttact cttcgttctt cttctgactc agaccatatt
     1501 cccgcaactt attggcaatc gcggtatgtg aaacgccgag acgttttgcc agtttgcgcg
     1561 tgctgggata attgcgataa agctgggtta ataccgagcg ttcaaaacgg ctggtgattt
     1621 cgtccagcga accttccatc gcatcttcgc ccacggctac cgttgcggcg tcataatccg
     1681 gcaacaaaat atcctgtgga cgcagctcat aaccgtccag ttgtgtcagt gcgcgataga
     1741 tagcgttctt taactgccgc acatttcccg gccacgcata acgcgtaagt acagtattca
     1801 ggtcagcggc cagtttcgga cgctgcacgc cctgctcgtc ggcaaagcgg gcgacgaaca
     1861 gctcagttaa cggcatgatg tcctgcggac agtcacgtag cggcggcaga ttgagcgtca
     1921 acacgttcag acgataatag agatcttcac ggaacatgcc tttttgcacc agttcgacca
     1981 gattcttctg cgtaacgcaa atcacccgca catcgacatg cacctcatgg tcttcgccaa
     2041 cccgacggaa agtgccatca ttaaggaaac gcagtaattt cgcctgcatc cgtggtgaca
     2101 tttcccctat ttcatccaac agcaccgaac caccgttcgc ctgctcaaag aatcctttct
     2161 tcccttccgg agcatgacca aacagttcac tctcgaccgc atcttccggt atagacgcac
     2221 agttcagcgc caggtaaggt ttgcccgctc tggggcttgc ctgatggcag gcgtaggcaa
     2281 agagatcttt acctgtacct gtgtcacccg taatcagcag cggcgcgctt agcatcgcca
     2341 gtttctgcgc ctgttcgaca acatgcttca ttttcgggct gacggcgaca atttgactga
     2401 aggcgctgac gtcctgggcg gcgacatttt gcaactggcg gcccatacga atcgttgatc
     2461 gcaacatcac caccgcaccg gtcaggacgt gttgatcatt ttcatcctga agataaacag
     2521 gcgtaatctc catcaggaaa ttctgcccat taataacgac atgctcgtta tgcgaatctt
     2581 gcggttcgct ttccagccaa cgtaaaaaat taaagccgtt aatcaattgt gcggcggtat
     2641 ggttgcgcag gcgatccaat ttttgcccaa aaagctgaca gctcgccggg ttcgccatat
     2701 ccactttgct tttcatatcg acagagagca caggttcagg caacgcctcc agtaacgcgc
     2761 tcaacgccag atgctcacgt tcggaaggca tccacgggac agtacgcaca tcggtaacac
     2821 ccgcaatacg gcgtatttcg gccatcagac tgctgaaact ctcaaactcc agttcagcaa
     2881 aattgaggta gattcgccca atgggatcaa tctcaatacc gcgtaaatca atgcctctta
     2941 gcacga
//
LOCUS       DAJTYZ010000266         2937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000266 DAJTYZ010000000
VERSION     DAJTYZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.271"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..262
                     /locus_tag="OO596_004207"
     CDS             35..262
                     /locus_tag="OO596_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6719872.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(263..637)
                     /locus_tag="OO596_004208"
     CDS             complement(263..637)
                     /locus_tag="OO596_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCR6719873.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OO596_004209"
                     /pseudo
     CDS             complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OO596_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2097..2672)
                     /locus_tag="OO596_004210"
     CDS             complement(2097..2672)
                     /locus_tag="OO596_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6719874.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          695 c          687 g          793 t
ORIGIN      
        1 ttatgccggt atttttttac ggaagaggta tcaaatgcgt ttaattgtgg tgagcatcat
       61 ggtgaccttg ctgagtggct gtggcagcat cattagccgc actataccgg ggcaggggca
      121 cggcaaccaa tattatcccg gtgtgcaatg ggatgtgcgt gactccgctt ggcgttatgt
      181 cacaatcctt gatctgccat tctccctggt ttttgatact ttactgctgc cgatcgacac
      241 tcatcatggc ccgtatgagt gattaacgtt catcccactc atcagctgca gcgtgtcctt
      301 cctcggtatc cagcggtggc tcaagctgaa attccccctc gtcccattca tgtaatgtat
      361 tctcttcctg ccactcctgg cggatctcta tctcatcata gtcgccatca aaaacacttt
      421 gcgcggcttc accgctaagc ataggtaaac attcaccttc ttccccttcg tcggcaaaaa
      481 actcaacttg ccacatgatg tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt
      541 gcacattcgc atcttcggcg tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag
      601 cgtcaatggc ttcttcaagc gttgcgtaca tggtcatcac gattctccct ttgagttgat
      661 gaggtttcag ggaaaaggat agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat
      721 cagttcgata cctggggtat tcgacgacga cgtagactgt tccatgaata gactgcgttg
      781 aataacacta cgccagcggt gacgagaaat accgctctga aaccgtagtt cgctgaaatc
      841 gctgctccca tcaatggtcc ggtaacgttg ccaatatcac gaaacgattg gttatagctg
      901 aagatacgtc cggcgatttg gttgctcgag ttataaacta acagtgtctg tacggcgggg
      961 agtagtgcac catcggcggc accgagcaaa aaacgtaaaa tcccaagttg caatggcgtc
     1021 tgaacgtaag acattgggat caacagcagt acagaaaaga tcagcgccgt aatcaggatc
     1081 ttttcgggtc cgattcgatc gccaagtttg ccgagtcgtg gtgcacttag cagagccgcc
     1141 acgcctggca ccgaggcgat catgccactg ataaaggcga cgttactgac gttacccgcc
     1201 agttcgcgga catacagcgt cagaatgggg gcaattgagc ccgtcgccac ctggatgatt
     1261 aacgtagtga caaacaggct gagtaccagt ttcgggtttt taagtgatgt caccacttcc
     1321 cgcatgtgca gcatctcttt tttgctgatc ggctggaatt tttctcttat gcaaaacagg
     1381 gtgacgaaaa agcagagtat gagcacactg gcggtaataa agaataccgg acgtaagccg
     1441 tagctatcgg cgagcaggcc gccagccatt gggccgagca acgcaccact aacgccgcct
     1501 gtggagagcg tacccagcgc ccagccgctt ttattacgcg gtacttgtgt ggcgataaga
     1561 gcattagcgt tggggacaaa tccgccaagt aacccaagaa gcgcccgcag gatcaaaaac
     1621 tgccagatat tttgtgccag ccccatcaac accatcacga tgcccatgcc gagggcagag
     1681 cgtaatagca tgagttttcg gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg
     1741 atggccgaaa ataaaaatgt aatgctcata tatgctatct taatgcacta cctgataagt
     1801 gagcctatac aggttattgc tgtatgttta tacagtatta ttggtagaag actgtgtaga
     1861 tttggtatag agatgaatag atactattaa ggtaacttat tgaaatcaat aaagattctt
     1921 gtgcagtctg aatagataac ttagcgttat gtctagtcgt ttccctctcc attcctaaaa
     1981 agtgctgtat actggcctat acaacgctat aaagacctct acatagcgcc ttgatagcta
     2041 tgtcacaggt acaaaaaagc cccctataaa ggaggctgtg tagtctatta agggtcttac
     2101 agggtcttgg tccagtgtct gctaacagtc cttacagaga ggttaagaac cttagctacc
     2161 tgagactgag atagtcccct ttccttgtac tctaagaccg ctctagttgt gtcactcgct
     2221 ataggtcttc caagtacctt accttgaact ctagcacgct ctagaccctg tttagtgcgc
     2281 tctctgatac ggtttctttc aaactctgca aaggtactaa atagctgtag aaccaactta
     2341 ccctctgagc ttgaaaggtc tttaacgggt aaatctaaac agatgagctt cacaccctta
     2401 tcattaagaa gggtcactgt attctgtagg tcgatgttat cccttcccag acggtcaagt
     2461 ttaagaacaa caagggtgtc accttcttct agcttatggt tgaccagatt cgcaaatccg
     2521 tccctatcca tagctgacac tgaaccagag acaacctcct ctataactcg tgacttagga
     2581 atatcatagc cagccttctt aatggcttga atctggttct ctgtggtgga agtctctact
     2641 gatacacggc agtaagcaaa ggttcgtgac atagatatcc ctctgggtag tcattaaacg
     2701 gttagtttgt cttgtctata gacattaaag caaatggtag atttgttgtc tatagctata
     2761 gtgactcttc tatgtagaca ttaaacggac gtttcttgtc gtgggtcaca tagatacttt
     2821 tagggcttct gtcaagtgat attcgtagat tttgcaaggg ggaggtgtaa ggctgaaaca
     2881 gggctactaa gaacacatac atctaaggct ggaaagtacc tgagcagata ttcttca
//
LOCUS       DAJTYZ010000267         2898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000267 DAJTYZ010000000
VERSION     DAJTYZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.272"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            223..1797
                     /locus_tag="OO596_004211"
     CDS             223..1797
                     /locus_tag="OO596_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6719875.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            1962..2681
                     /gene="fliA"
                     /locus_tag="OO596_004212"
     CDS             1962..2681
                     /gene="fliA"
                     /locus_tag="OO596_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6719876.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            2727..>2898
                     /locus_tag="OO596_004213"
     CDS             2727..>2898
                     /locus_tag="OO596_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="expression activator of the class 2 type of
                     flagellar operons, essential to achieve maximal cell
                     motility; activator of type 1 fimbrial gene expression;
                     cell density-responsive regulator; FliZ in Salmonella
                     typhimurium induces HilA, an activator of invasion genes
                     necessary to achieve full virulence; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulatory protein FliZ"
                     /protein_id="HCR6719877.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLD"
BASE COUNT          855 a          640 c          690 g          713 t
ORIGIN      
        1 agcgggttat cggcaatttg tgcgcaaagt ttaatagttg attcacgaag atgatgcgca
       61 gagtagagtt gtatattacg gatatttcag ccgttaagaa aaaacaggag agaaactaaa
      121 attttctaaa gttcgaaatt caggtgccga tacaaggggt acggtgagaa accgtgggca
      181 acagcccaat aacatcaagt tgtaattgat aaggaaaaga tcatggcaca agtcattaat
      241 acaaacagcc tgtcgctgtt gacccagaat aacctgaaca aatctcagtc ttctctgagc
      301 tccgccattg aacgtctctc ttctggcctg cgtattaaca gtgctaaaga tgacgcagca
      361 ggtcaggcga ttgctaaccg ttttacagca aatattaaag gtctgactca ggcttcccgt
      421 aacgcgaatg atggtatttc tgttgcgcag accactgaag gtgcgctttc tgaaatcaac
      481 aataacttac agcgtattcg tgaattgtca gtacaggcca ctaatggtac aaactctgac
      541 tccgacctga attcaattca ggatgaaatt acacaacgcc ttagtgaaat tgatcgtgtt
      601 tctaaccaga cacaatttaa tggtgtaaaa gttctggctt ctgatcagac tatgaaaatt
      661 caagtaggtg cgaacgatgg tgaaaccatt gagattgccc ttgataaaat tgatgctaaa
      721 accttggggc ttgataactt tagcgtagca ctaggaaaag ttccaatgtc ctctgcggtt
      781 gcacttaaga gcgaagccgc tcctgactta actaaggtaa atgcaactga tggtagtgtg
      841 ggaggtgcta aagcattcgg tagcaattat aaaaatgctg atgttgaaac ttattttggt
      901 accggtaatg tacaagatac aaaggataca actgatgcga ccggtactgc aggaacaaaa
      961 gtttatcaag tacaggtgga agggcagact tattttgttg gtcaagataa taataccaac
     1021 acgaacggtt ttacattatt gaaacaaaac tctacaggtt atgaaaaagt tcaggtgggt
     1081 ggtaaggatg ttcagttagc aaactttggt ggtcgtgtaa ctgcatttgt tgaagataat
     1141 ggttctgcca catcagttga tttagctgcg ggtaaaatgg gtaaagcatt agcttataat
     1201 gatgcaccaa tgtctgttta ttttggggga aaaaacctag atgtccacca agtacaagat
     1261 acccaaggga atcctgtacc taattcattt gctgctaaaa catcagacgg cacctacatt
     1321 gcagtaaatg tagatgccgc tacaggtaac acgtctgtta ttactgatcc taatggtaag
     1381 gcagttgaat gggcagtaaa aaatgatggt tctgcacagg caattatgcg tgaagatgat
     1441 aaggtttata cagccaatat cacgaataag acggcaacca aaggtgctga actgagtgcc
     1501 tcagatttga aagccttagc aaccacaaat ccattatcca aattagacga agctttggca
     1561 aaagttgata agttgcgcag ttctttgggt gcagtacaaa accgtttcga ctctgccatc
     1621 accaaccttg gcaacaccgt aaacgacctg tcttctgccc gtagccgtat agaagatgct
     1681 gactacgcaa ccgaagtgtc taacatgtct cgtgcgcaga tcctgcaaca agcgggtacc
     1741 tctgttctgg cacaggctaa ccagaccacg cagaacgtac tgtctctgct gcgttaattt
     1801 acacttatcg ctcaaacccc gccaccggcg gggttttttc tgtccgcaat ttaccggtaa
     1861 cccctaaata acccctcatt tcacccacta atcgtccgat taaaaaacct gcagaaacgg
     1921 ataatcatgc cgataactca tataacgcag ggctgtttat cgtgaattca ctctataccg
     1981 ctgaaggtgt aatggataaa cactcgctgt ggcagcgtta tgtcccgctg gtgcgtcacg
     2041 aagcattgcg cctgcaggtt cgactgcccg cgagcgtgga acttgacgat ctgctacagg
     2101 cgggcggcat tgggttactt aatgccgtcg aacgctatga cgccctacaa ggaacggcat
     2161 ttacaactta cgcagtgcag cgtatccgtg gcgctatgct ggatgaactt cgcagccgtg
     2221 actgggtgcc gcgcagcgtg cgacgcaacg cgcgtgaagt ggcacaggca atagggcaac
     2281 tggagcagga acttggccgc aacgccacgg aaactgaggt agcggaacgt ttagggatcg
     2341 atattgccga ttatcgccaa atgttgctcg acaccaataa cagccagctc ttctcctacg
     2401 atgagtggcg cgaagagcac ggcgatagca tcgaactggt tactgatgat catcagcgag
     2461 aaaacccgct acaacaactg ctggacagta atctgcgcca gcgggtgatg gaagccatcg
     2521 aaacgttgcc ggagcgcgaa aaactggtat taaccctcta ttaccaggaa gagctgaatc
     2581 tcaaagagat tggcgcggtg ctggaggtcg gggaatcgcg ggtcagtcag ttacacagcc
     2641 aggccattaa acggttacgc actaaactgg gtaagttata acgtcagtaa atgccgcact
     2701 ttaattttga ctaccaggag ttcttaatga tggtgcagca cctgaaaaga cggccattaa
     2761 gccgctatct taaagacttt aaacacagcc agactcattg cgcgcattgc cgtaaattac
     2821 tcgaccgcat taccctcgtt cgcgacggca aaatagtgaa taaaatcgag atttcccgcc
     2881 tggacatgct gcttgatg
//
LOCUS       DAJTYZ010000268         2888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000268 DAJTYZ010000000
VERSION     DAJTYZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.273"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            22..1104
                     /locus_tag="OO596_004214"
     CDS             22..1104
                     /locus_tag="OO596_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6719878.1"
                     /translation="MFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTFTAQKAVI
                     AIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKTL
                     RAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDALV
                     KGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLIR
                     PGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGKL
                     TSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYSD
                     HFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1564..2001)
                     /locus_tag="OO596_004215"
     CDS             complement(1564..2001)
                     /locus_tag="OO596_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719879.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2090..>2888)
                     /locus_tag="OO596_004216"
     CDS             complement(2090..>2888)
                     /locus_tag="OO596_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6719880.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          759 a          625 c          643 g          861 t
ORIGIN      
        1 ctggcgtggg cggtctttaa aatctttcgt attccggtga atcagtggac gctggcgacg
       61 gcggcgctgg gaggcgtgtt tctggtgagt ggtttgattt tgttgatgaa ctacaaccac
      121 ccttacactt ttaccgcgca aaaggcagtg atagcgatcc ctatcacgcc acaggtgacg
      181 ggaattgtta ctgaagtcac tgacaagaat aatcagctta ttcaaaaggg cgaggtgctt
      241 tttaagctcg acccggttcg ttaccaggcg cgagttgaca gacttcaggc tgacctgatg
      301 acggcgacgc ataatataaa gacgctgcgt gcgcagctca ctgaagcgca ggccaacacc
      361 acccaggttt cagcggagcg cgaccgtctg tttaaaaatt atcaacgtta cctgaaaggc
      421 agccaggcgg cggtgaatcc gttctcggaa cgtgacatcg acgatgcgcg gcaaaatttc
      481 ctcgcgcagg atgcgctggt gaaaggctcg gtggcggagc aggcgcagat ccagagccag
      541 ctcgacagta tggttaacgg cgagcaatcg cagattgtga gcttaagagc gcaacttact
      601 gaagcaaaat ataaccttga gcagactgtc attcgcgcgc cgagcaatgg ctacgttact
      661 caggtactga tccgcccagg tacatacgca gctgccttgc cgctgcgtcc ggtgatggtc
      721 ttcatccccg agcaaaaacg gcaaattgtc gcccaatttc ggcaaaactc gctgttacgt
      781 ctgaaacctg gcgatgatgc ggaagtggtg tttaacgcgc tacctgggca ggtgttccac
      841 ggcaaattga ctagtatttt acctgtcgtg ccaggcggtt cttatcaggc gcagggggta
      901 ttgcaatcat taacggtcgt tcccggcacg gacggcgtgc tgggaaccat tgaactggac
      961 cctaacgatg atatcgatgc cttacccgac ggcatttacg cccaggtagc agtttactcc
     1021 gaccatttca gccatgtttc ggtgatgcgg aaggtgctgc taagaatgac cagttggatg
     1081 cattatttgt atttggatca ttgaggggga agggggagat tatggaactt tgctccctgc
     1141 aagggctaat tatttttgca tattacttca ttggctgcga tgatttttct ttaatattca
     1201 ttatgttaat aagttctgga tgagtgtgct ctcattggat tttttatccg tcttgttctg
     1261 ccgcaggatg agactataca tcgtcgagat cgcaatgatg atatgagttg ttcctcctgc
     1321 gacggctcta atcaaaaaag agcaggagtg cttttccatc tattgcgatg atggctttat
     1381 tatctgaagg atgacagtca catttaaaaa atatttaatc tatagatcac aatgtagggt
     1441 catggaatat atgtatacaa ctattttctt cttcactttt aattatcgta ttcattataa
     1501 aactaagtta aaaaaactcc tagagagtga gttattaata tatttttaaa taaaattaag
     1561 ttatcattca tcgatgcatt ttatagaatc atcaatggcc ataatagtac tattaactct
     1621 caattttata tttttatcat cactacaaat attaactgaa tttatatcaa taattttttt
     1681 attcttatct tttattttat ttactgtttc aatgcaatca gcggcaagtt ggagtggatt
     1741 tagatttttc agatatggtc ttatgatctt tcccttactt aataaatagc atgtatagga
     1801 ttcggaaagt ccagcatcta ttttgtacct cattatatta gataaggatg tgagaaattc
     1861 cttgttatca ttcgcagcca tatatgccag tccgagttca gcctctgcgg ccttacaggc
     1921 aaaatagctt agtgaacacg cttttcgtcc ctctttagta ctggctgata ttagtgccca
     1981 taatattgct ttgtctgcca aatctaactc tctctgaaat tgtcttgcag taactgcact
     2041 cgcagaaata gagaaaaaaa tacaattatt aataactttg ccatattttt tagtccgtaa
     2101 ctgttaatgt gctatctggc tctgaaaata ttaactcatt taatttttgc atgtcattag
     2161 atgaaccagt tatacaaccc tcggaacaga atcgaggtcc taaagacgga ccgaaagcgt
     2221 ttaaacaact ttgtgttaaa aatccatcac gattatatgt ttctgtattt gctgacggta
     2281 ctaaacgtat accattaggt tttctattgg ttgctccagg accattaaca ttccagctcc
     2341 ataccccttg aggtattgga cctctatttt gtaagtgtgt acaaccagga ttatttttac
     2401 attgcatatt attaccatta ttaccagagg cgacaggaat agtaagcaca tcataagagt
     2461 tatcatcatt ttgcgtatct gaaacgcatg aaagcatact taaagttatg ctgtataaac
     2521 aggtcgctaa tcccaacggg tcaatatcag ttatcggatt caacggatac tgataaaaat
     2581 tccatccccc cttcagtcca atcggatcct gagtgatata tcgcccctgc agcgggtcat
     2641 aatagcggtg gcggttgtaa tacaggccgg actcctcatc atactgctgc cccggcaggc
     2701 ggataagctg ctgcagctga tgcgggttct cttcattcag caggttgccc cactcatcgt
     2761 attctgcgca ccattctgtt gccccttccg tgctgataag caccagcggc aggccgcgat
     2821 ggtcgcagtg gtacaggtgg atttttcgcg ccggcgtgta caccgggtcc atctggtttt
     2881 gcatctgc
//
LOCUS       DAJTYZ010000269         2882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000269 DAJTYZ010000000
VERSION     DAJTYZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.274"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            63..170
                     /locus_tag="OO596_004217"
     CDS             63..170
                     /locus_tag="OO596_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6719881.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(257..1936)
                     /gene="bcsG"
                     /locus_tag="OO596_004218"
     CDS             complement(257..1936)
                     /gene="bcsG"
                     /locus_tag="OO596_004218"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6719882.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1933..2124)
                     /gene="bcsF"
                     /locus_tag="OO596_004219"
     CDS             complement(1933..2124)
                     /gene="bcsF"
                     /locus_tag="OO596_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6719883.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2121..>2882)
                     /gene="bcsE"
                     /locus_tag="OO596_004220"
     CDS             complement(2121..>2882)
                     /gene="bcsE"
                     /locus_tag="OO596_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6719884.1"
                     /translation="QQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLL
                     ACGANMVIPWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVF
                     CNAVNNMMNNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLF
                     LSFCRINDLDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPL
                     TQSSKPVINAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT          723 a          739 c          768 g          652 t
ORIGIN      
        1 tcttgaaccc aaacaactca agaatagccg cgaaacgttg gcattacaac acaggcggct
       61 atatgacgct cgcagaactg ggcatggcct tctggcatga tttagcggct ccggtcattg
      121 ctggcattct tgccagtatg atcgtgaact ggctgaataa gcggaagtaa cgtgtcatgc
      181 gggcgtcagg ctgccgtaat ggcaatttgc gcccggatca ggccgcagag gttaaactct
      241 gcggcctttt tcgtgcttac tgcgggtaag gcacccagtc gccgccgttc aggcgaacgt
      301 acggtttatc ctggtattga ataactactg catttgagtt ctcggagacc ggtgctgttt
      361 gtggcaaccc actggtgagt tttttccagt caacattgtc ttcggtgaaa atcttgccat
      421 caagaacgcg aaccaccaga tcggagatag ctaggaagct gctcggttgg tcaatgacaa
      481 tcggtgcccc ctgatgtggt gccttcatgc cgaagaattt caccccaacg gggacgtctg
      541 tgatagacgg gctagggata tcacgtaggc cagatacctg cattctgtcg cccttcagcg
      601 cgccgccgtg ttccggcacc acgaccacca tcactttacg acccgatttc tccagttcag
      661 taaagaaggc gtccagttca tcaaagaatt tctgcgcccg cgctttgtaa tccgctgttt
      721 tgctgacccc cggataatgg ttgccgtcat gcagtggaag cgtgttgtag aacgtggcac
      781 tacggctgtt tttatctttt tcggtaacgt ccagccagcg gttaagcacg gcggtatcgt
      841 cataaaccgg cgaaccatca aagcccagca aaataaccgg cagatttgtt tgatccatca
      901 attcagtctg catgccgcca ttttcgcgaa cttctttcaa aaaaccgccg aactggccgt
      961 tatgtcccat catcaggtgc tgggtaaagc ccagtttcga aaggttatca aacagatagc
     1021 agtcgttatt tgccggttga tacagattag tgtgcgaagt ctgcccgcag ctggcgcgca
     1081 gtaaacggat cgccgccggg ccactgtagg aggtggcgga gttaaagttc ttgaactcaa
     1141 tatcgaaatg cgaccacagt ggatgcgaca tcaacccggc ggcttctata tccgaccagg
     1201 aaagcgaaca gatgttaatc accagtagtt caaatggctg agcatcagcg ggcagcgaag
     1261 acgggaaggt cgatttacgt ttcgcctccg cgttatagaa attattcagc caggcgttaa
     1321 ggttcgccgt tgttggcggt gcagtttgtg cgggcatatc acccactacc gatgcgccac
     1381 ccgtcgccgc aacggttgcc gctgcgttac cacccgtcgt tgttacagtg gtcgtcggtt
     1441 gtccggctgg ccacaaggag aaacttggtc ccgccagggt aagtacgttc agccatagca
     1501 gtatggcaac cacaaaaacg gtaatgcgaa tccattgtga caggaataac caggccacta
     1561 ataaaacaaa aatggcccca atcatctgcc agttaataaa gcgtgtgaca aggtcgatta
     1621 aataatcggt actgaacccc gccacctgcg aaccctggct cattatgctt tccgggccag
     1681 gcaaccaggt gtcatgccag aacaaagcaa agccgatcgg cagggcaatc cagtggcgca
     1741 agcgatgcag gctgtagcgc ggaatgggca tcagcagaaa cgtggcaaac accaaattga
     1801 ggagcggatg gaagttaaga tatcccgccc acaacaggcc gaacttaacc agaaaataga
     1861 agttccagcc ggaaaggccg cgccagtatt gccagaggga agaaggcatg gcggtatttt
     1921 gcgtaaattg agtcattttt tggttgccct ggctttttcc gtgcggcgta acgtcccggc
     1981 cggtttaaca taacgaggtt tagcaaagaa taaacgtaag gtgtcgcgaa tgcgtcgcaa
     2041 aaagtgccgc gccagatagc ccagcgggaa aaatatcagt gcgcaaacga caataatttc
     2101 aatgatatcg ctgatggtca tcatgatgag cgctccacag catcatctaa cagtcgcatg
     2161 ggttctggta ttcgtcgcca gtggcgacca tcgtgctcgg cattgatgac cggtttagaa
     2221 ctttgcgtta aaggcagcgg catgccccat tgttctgggg caagcaagcg catctgcacc
     2281 agctcggcac tgatttgatc atcttcaaac cagaccatac ggtttgagaa aatgtcgcca
     2341 gtaggcaatg ggaaaatatg attcaacgcg gtatccagat cgttaatccg acagaatgag
     2401 agaaacagca ccagccgatt accgccaatg gtcatgatat cgccggtacg gttagggcga
     2461 cacagcgtca gggcttgttc aacgcggata cccggtaccg gacgtagggc aaccagaacg
     2521 cctttaccgt gggcaggtaa tagagggtta ttcatcatgt tgttgacggc attacagaac
     2581 acatcccact tctggaaacc acgcagtttg agcggctggg tcattgacag caaggtagtg
     2641 atatcttccg gcacatagcg actaaacttc tgcccttgca cgctttcgat catcgtcaga
     2701 caacgggaga gtggcgcatt ccacggaata accatatttg caccgcaggc caataacaaa
     2761 cgttcatcgg tggcgcgcag gctagcggta ttttcccgca cgaggatttt catcgcacta
     2821 ccgcgctggc gacgcagggt atgaatgctg cgggccagtg gctcgatttg cgcattttgc
     2881 tg
//
LOCUS       DAJTYZ010000270         2868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000270 DAJTYZ010000000
VERSION     DAJTYZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.275"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..158
                     /locus_tag="OO596_004221"
     CDS             <1..158
                     /locus_tag="OO596_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6719885.1"
                     /translation="REMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(204..620)
                     /gene="rraB"
                     /locus_tag="OO596_004222"
     CDS             complement(204..620)
                     /gene="rraB"
                     /locus_tag="OO596_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6719886.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            782..1786
                     /gene="argF"
                     /locus_tag="OO596_004223"
     CDS             782..1786
                     /gene="argF"
                     /locus_tag="OO596_004223"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6719887.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(1858..>2868)
                     /locus_tag="OO596_004224"
     CDS             complement(1858..>2868)
                     /locus_tag="OO596_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6719888.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLF
                     FNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSI
                     LVNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPG
                     YVVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGW
                     NN"
BASE COUNT          810 a          638 c          573 g          847 t
ORIGIN      
        1 cacgagagat gggcttcaaa cgctgctatc tggaaacgac cgctttttta aaggaagcca
       61 ttgcgcttta tgagcatttg ggctttgagc atatcgacta tgcgcttggc tgcacgggcc
      121 atgtcgattg tgaagtgcgg atgctacgcg aactctaaaa aaatgcccgc tagtatgtgc
      181 tatcgggcat tttaatcaat taattagtgg cgaaccccgt catcgtcttc atcgacaaaa
      241 tcttcatcgt cgccatcttc gccgttagga tcttcaaagt aagtgcccca accgtcgtac
      301 tcaacgtcaa atttctccgc cagcgtcatc agttgttcaa cctgggcatc gatcagatcg
      361 gcattcaacg cgcactcgct gaggatgtcg cagcaaatca cgatatcacc atcttcaact
      421 tccagctctt ctggatcggt cacttcgtaa ccgagtttaa acgcttcaac tgccgctttt
      481 tccagagttt ccagatcgtc tgcggaaaga tggtgttcga tggtgtacaa cgcgtccgga
      541 tcgctgccat cttccagtaa ttcttcaata atcaaacgtg tttcttcacg ctgttcttcc
      601 agttgttccg ggtttgccat ggctcattcc tcttaatgtc ctgccgatac tcttattgtc
      661 acacaccact gacattgcct ccacctttgt gataaagatt tatgctttag acttgcaaat
      721 gaataatcat ccatataaat tgaattttaa ttcattaagg cgttagccac aggagggacc
      781 tatgtccggg ttttatcata agcatttcct gaaattactc gatttcacgc cagctgaact
      841 caacagcctg ctgcagttag ccgcgaagct gaaagccgat aagaaaagcg gtaaagaaga
      901 agccaaactc actggtaaaa acatcgcgct catcttcgaa aaagactcga cccgtacccg
      961 atgctctttc gaagttgccg catatgacca gggggctcgt gttacttatc tcggcccaag
     1021 cggcagccaa attggtcata aagagtcgat taaagacact gcccgcgtgc ttggtcgcat
     1081 gtatgacggt attcagtatc gcggctatgg tcaggagatt gtcgaaacac tggcggaata
     1141 cgctggcgtg ccggtatgga acggcctgac caatgagttt caccccacgc agctgctggc
     1201 ggatctcctc accatgcagg agcatttgcc cggcaaaacg ttcaacgaaa tgacgctagt
     1261 ctatgcaggt gacgcacgta acaacatggg caattcgatg ctcgaagctg cagcgcttac
     1321 cggtctggat ttgcgtctgg tcgcgccaca ggcatgctgg ccggaagctg cgctggttac
     1381 ggaatgccgc gccctggcac aacaaaatgg cgggaatatt acgctgacgg aagatgtagc
     1441 taaaggagtt gaaggtgctg actttatcta taccgatgta tgggtgtcga tgggtgaagc
     1501 aaaagagaaa tgggctgaac gcattgcatt gctgcgtgat tatcaagtga acagcaagat
     1561 gatgcagttg accggtaacc cggaggtcaa attcctccac tgcctgcccg cgtttcatga
     1621 cgaccaaacg acgcttggca aaaaaatggc ggaagagttt ggcctacatg gcggaatgga
     1681 agtgactgat gaggtcttcg aatctgccgc cagcattgta tttgatcagg cggaaaaccg
     1741 catgcatacc atcaaagcgg tgatggtcgc gacgctcagt aaataattga tatcgctata
     1801 aagatgccgg gaaatacaaa aacatcccgg cgtcacatta ttttatgcag tgatagttca
     1861 attattccac cccaattttt ttattgctat acgtaaatct tccggaggat ttaattttcc
     1921 taactgttct tcattaatat atccatatat attgattttg ccattccgga ggattttcat
     1981 cattcttgtt ctaattttta attcattacc catcttttct tcaaaaatct taacgacata
     2041 acccggaagt ttatttacaa tttcttttgt tttttcttca tcaccataaa catatcctaa
     2101 ctcattaaga tacagctgtt cgccctcata acacttaagt aattgataga agtaccactt
     2161 agcaaaatca ggttgcttca tgaaaaaatc cttcaaaaaa atatcattta ctaaaatgct
     2221 gacaggcact tcattaaata gtaacttaac atcatcaaat aaccttagta ttaagcgtga
     2281 tttttcttga ttgcttaagc taaaatcatt gacatatgca accaacttat cccaaatcaa
     2341 ttcctgtaat ttacggctat ttttataggg ttctttatcg agattgaaaa ataaggtctc
     2401 agctatttta attttagatt gatcgcagct aaaatcttta gattgatcgc agctaaaatc
     2461 aatatgattt atcacggagc atgcatactt tatcttttca gaatcaccaa aatattgcat
     2521 tcttttatta aaacaccaat ccagtattct tacatcatta atatatgggg attttaaaaa
     2581 agtctccaga atcgaatcga tgaccggtgt aaggttaact tttgcatcat ctaaggaatc
     2641 aataagaata tgagacaggt taactttgat gtcattaatt tcattgtcag tagctttctt
     2701 attattaaga agataattat ttactgcgtt aaggtcatct atttctttca gtatcccttt
     2761 ttcaccaagc aagtatgaaa tcgattcatt acatacataa ggcattaata gcagatgttc
     2821 tttccttatt gctaataatg cattgttcaa tggtttttct aataaatc
//
LOCUS       DAJTYZ010000271         2846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000271 DAJTYZ010000000
VERSION     DAJTYZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.276"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..992)
                     /locus_tag="OO596_004225"
     CDS             complement(<1..992)
                     /locus_tag="OO596_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6719889.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQL"
     gene            complement(1149..1358)
                     /locus_tag="OO596_004226"
     CDS             complement(1149..1358)
                     /locus_tag="OO596_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719890.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1381..2091)
                     /locus_tag="OO596_004227"
     CDS             complement(1381..2091)
                     /locus_tag="OO596_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6719891.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2607..>2846)
                     /locus_tag="OO596_004228"
     CDS             complement(2607..>2846)
                     /locus_tag="OO596_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719892.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          836 a          591 c          467 g          952 t
ORIGIN      
        1 aataactgaa aagcacctgc cattctgtca cctaattgac cgcgtagctc ttcagcctga
       61 atctttcctt tagatgccat ctgctcgaaa gcttttaaaa tcccgttaat ttcatcggtg
      121 cttagcccca ttaatctgga tgtaccaatc acattagaaa ataaggattc tttctggtct
      181 gcattcatat caccagcacc agcggaaaac ttagcaaaac tgcttaatgt ttcaatctgg
      241 tctacaccac tactatcagc aagattctta acaaattctg tagctcgttt agcaccttca
      301 tcacctaaaa cgaattgtgc cgctcgttgt gatgcttctt ttttcagacc aacctccata
      361 atccgcctgt aagcttctaa ggctgtctga aaactataaa tggctgtacc tgctttgatt
      421 gcactcgtag ctaatacatc cagactattt gaagcatttt cactgtgatt atttttgctg
      481 gtggtcttgt taaacttaga aagtgtttca cgaagagact gatttaactt tgctaactca
      541 cgattaaaag tttcgtttcc aagtgtcttg gatttactgc ttaagctgta atactggctt
      601 ttaaaatact tctcaagcat aggattaaag tcacgaagct tgctcatatt cgctgagtaa
      661 gctgctgtga ttgctctgct tccgctaatt cgtggctgtc ttggttgtct aagtggcttt
      721 aaagtatcct caatttcagt tttaatctta aacgctctag tgtttttaag acgctgctca
      781 atcttggaaa cagttttatc gacatttctt tccattagtt tgagagaatc atttaaaggc
      841 gtaggattaa ctctaaacga cttattcaat cgcttctcaa ttcgtgatgc tacctgcatc
      901 gtttgtttct cggctttttc caagcctttt aaaactttct gagagttaaa acttaactca
      961 atcagaaaat catcaacttt aaattgtgcc attcatattt ttcacctctt taagcgtgtt
     1021 gtacgtaaat tctatttttc agtgtgggta ttagctttac agatttcacg tagcacacat
     1081 acaaaaaaag ggagagctac cgatgtcact cgataatctc tccccgtatt tacccaacca
     1141 attctttttt aatgtcggac aagataatgt aattccgttt cacctatgat ttgagcatca
     1201 gaaggaatac ggtcaataca ccactttgga atcttgcgtg tttttggttt gtgtgaggtg
     1261 ataacttgac gtttgttttt aattcttaag gctggcttag tttgtaactt taagttttca
     1321 caaacagacc gaatagtctc taaacgttct tcaaacatta atcacctctt taattcataa
     1381 tcaaacagcc ttgctatact tctgagaaat cttcgttaac tgctcaatat cagccatcaa
     1441 agcaatcttc ttctgcgact catattcact aacaagttct ttcatagtat caagttcttt
     1501 tttaagcttt tgctttaact tatccagttt accggaataa tgcttagtca aattagcttc
     1561 agctaactga agaatctctt catctgtttt atcagtcatc agaataaatt gaccagaacc
     1621 ccttaatgct tgtctgacat caattaaccg gaacccttca gggatacttt gaagaacttt
     1681 aggaacgtct tcaatcgctg taccaaaacc atcattaaag tttccttcat aatagataaa
     1741 cgggtttgac ttaatctcgt aagcaatatc atattctgaa ggtttcgagg tttcttcgaa
     1801 gttcttaaca gcatcgtaag cgacatcaag tctaagacgc tgtgatttta aagctgggta
     1861 atattcacgc tcaagctcaa atagtgcttc agttttgcag atatcaggca tttcttgagg
     1921 ctcaggagac acaaaggaaa ttacctttgg tgacatcttt agctcttgct caagctcaat
     1981 gcgcttgtca tgctcttgac gctgaatagt atgtagcgga tgttcaccct gtggtgacca
     2041 gtctccgaag ctatcaacct ttttaccgtt aatatataaa tcgtttgtca ttataatttc
     2101 ctctaagtgt ggttatttct taatagagca ccttcaacgc caatacactt agaagatgac
     2161 ttaacgattt attaaagatg ctctattaag aaaagcccta aacataatct gtgaagggct
     2221 ttggaggttt gcttataacg ttaacgaagt ttcaggaaaa ctttctttaa aagctctgtt
     2281 taaaaactct taaagaaagc cctaactcat gattagggca aagctgtaaa ggattcaata
     2341 atgaatgaac aaagactttt tgagatagcc tctcagacca acctgaaagg ctaggacgaa
     2401 aagcattgca cctttccaac tcagaatatc cattcatcgg attattgaaa gaaaaaataa
     2461 gagagaattg ctctccccca tatatacaat tatgtcatgc tcaaggttgt ttgtcaacag
     2521 cctttaacag attggtaaca tttatacttt aatagatgca cctgcaatca taccaatcaa
     2581 tacaaaacag ataatcacca attccattaa tgcaccacct tcgaatccgt caccagaata
     2641 ttatcgctat acaaaattgc tatagccgcc ttgagatatt tttcagggct aacgctgtat
     2701 ttgttcgttt cttcgataat aagacgcttc atgtttgagt cggtaacagg aaacataaaa
     2761 cgcggttcac cagtttcaat ttcctgaata aagtcttcaa tttcttctga gttttcagcc
     2821 attagcttta gtaatttatc aaggtc
//
LOCUS       DAJTYZ010000272         2815 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000272 DAJTYZ010000000
VERSION     DAJTYZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.277"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..989
                     /locus_tag="OO596_004229"
                     /pseudo
     CDS             1..989
                     /locus_tag="OO596_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1005..1754
                     /locus_tag="OO596_004230"
     CDS             1005..1754
                     /locus_tag="OO596_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6719893.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            1756..>2815
                     /locus_tag="OO596_004231"
     CDS             1756..>2815
                     /locus_tag="OO596_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719894.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          886 a          566 c          585 g          778 t
ORIGIN      
        1 aggttaaata catacgtaag tttggctcgt aatacttact gggacgccag cagtaatgtg
       61 aattattcat tatcacttag ccgcgatttt gatatcgggc cattaaaaaa cgtctccgct
      121 tctctaacat ttagccgaat aaactgggaa gaagacaacc aggatcaact gtacctaaat
      181 atttcgattc cctggggaac cagtagaaca ttgagctatg gtatgcaacg aaatcaggat
      241 aacaagattt cgcatactgc ttcgtggtat gactcttccg atcgaaataa ttcctggagc
      301 gtttctgctt caggcgacaa tgatgaattc aaagatatga aagcgtcact acgcgccagt
      361 tatcagcata ataccgagaa cggtcgtctc tatctctccg ggacatcaca gcgagacagt
      421 tattattctc tgaatgccag ttggaatggt tcattcacgg cgactcgtca cggagccgct
      481 ttccatgact atagtggtag tgctgactcg cgttttatga tcgacgcaga tggtgcagaa
      541 gatattccgt tgaacaataa acgcgcggta actaatcggt atggcatcgg agttattcca
      601 tcagtcagca gttacataac aacatcatta agcgttgata cccgaaatct tccagaaaat
      661 gtggatatcg aaaactcggt tatcaccacc accttaaccg aaggtgctat tggctacgcc
      721 aaacttgata ccccaagggc tatcaaatca tgggggttat tcgcctggca gatggtagtc
      781 acccaccact ggggattagc gtaaaagata aaaccagcca taaagaatta ggactggttg
      841 ctgatggcgg ctttgtatac ctcaacggca ttcaggatga cagcaaactt actttacgct
      901 ggggtgataa atcctgtttt attcaaccac ccaatagcag caacttaacc accggaacgg
      961 ttattttacc gtgtattagc caaaattaat tcaggatgaa actaatgcgc tacttaaaca
     1021 caaagaatct cattgcagca ggagtattac tcgcctgcat gagcagcatt gcctggggtg
     1081 ccatcatccc ggaccgtacc cgtatcataa tgaatgagtc agataaaggg gaagcattaa
     1141 aacttactaa tcaaagcaaa aatctaccct atctggcgca aacatggata gaagatacga
     1201 aaggcaataa atcacgtgac tttattgtca cggttccccc tatggtacgt ttaaatccca
     1261 gtgagcaaat tcaaataaga atgataagtc aggaaaaaat tgctcaactg cctaatgaca
     1321 gagaaacgct cttctatttt aacgtgcgag aaataccgcc aaaaacagat aaaaaaaatg
     1381 tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa gctattgaat
     1441 tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga aatgatggtt
     1501 cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc ggaacaaacg
     1561 gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc catgcagata
     1621 cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat tttggtggat
     1681 taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt tccgtagcca
     1741 aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat aggttttagt
     1801 gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa taccgtagtc
     1861 attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga taagatctgg
     1921 caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc cccatcacct
     1981 ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg cggtcaggac
     2041 ttttataatc agaactccta tatttttggc gtaacataca atggagttga ttatgattcg
     2101 actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc gggtaccttt
     2161 aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca attatcagta
     2221 acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa tagagttaat
     2281 aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat gagtggcgca
     2341 ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa taatattcat
     2401 gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt tgactttggt
     2461 aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag tgtagtcatg
     2521 acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag ctttttcact
     2581 acggatattt tgagtgatta tagccatctg gatatgggta atggactgct attgaagata
     2641 tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc ttctttttca
     2701 tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc gaacccggca
     2761 gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac ctata
//
LOCUS       DAJTYZ010000273         2777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000273 DAJTYZ010000000
VERSION     DAJTYZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.278"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..411
                     /locus_tag="OO596_004232"
                     /pseudo
     CDS             1..411
                     /locus_tag="OO596_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            996..1712
                     /locus_tag="OO596_004233"
     CDS             996..1712
                     /locus_tag="OO596_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719895.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYILARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2264..2668
                     /locus_tag="OO596_004234"
     CDS             2264..2668
                     /locus_tag="OO596_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719896.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
BASE COUNT          806 a          531 c          609 g          831 t
ORIGIN      
        1 gaagacccag atatgaacag gcacattcag agttacttta aagagcttac tgaacgtctg
       61 aggtgttaga gccatgactg ccgcagcatt gctggcggaa atgcctgaat cgagcagcct
      121 ctcgagacgt gaaatcagcg cacttgtcgg tgttgctcag gtaaaccggg actcgggaac
      181 tctgcgcggt cgacgaacca ttttcggtga ttgtgctggg gaagaacagc tctgtacatg
      241 gcggcgcttg aggcaccccg gtttaatctg gtgataaagg cgttttacat gcgcctactt
      301 gcagccggaa acgccaaaaa agtggcactg gttgcctgta tgcgtaagtt gctgaccatt
      361 atgaacgcaa tgctcagaaa aaacgaagaa tggaatgaat cgtatttatg aaaactggat
      421 tgtgggccat aaatattcca aaaagatagc ccgctccgtt tattaccgga acgggcgaga
      481 gcatgacatt gtctgtaatt ataatgagac tgctgattct acttattttt aatttcaaga
      541 aaagcgtttt ctacaaagaa ttatgagatt taatgttttg tttagacaat attatcattc
      601 tgttcaataa tgaaagtctt catcaacaac aagtaaaaac aacgtccctc tcatcgaacc
      661 ggacttgcag atttcccgca tccggctctc ttgcaacaca tcagaccttc acgttcgggc
      721 ggttatgggg aaggtccagc aagctgtatg agccctaact gtttgaatac cagtagatta
      781 ccagaaactt ctatcgattg aagacattac caactagcgt tactaaatgt tgaccaagtt
      841 ttgttcactt gtctggtttt gatagtagca ttctgcagag tattttgata tgataaatat
      901 gcattaacaa tgatattatt gttaatttcc tagcatgaaa attaaaagat caacttttta
      961 tctcgaatat tttttacatt atcagttggt ttcttatgaa tgacaattca ttgcttagaa
     1021 attcttcatt attcatcgcc tatatgggat gtgtagggtg ggtcagtgct tattcttatg
     1081 gctggggaac ctctttttac tatggatttc catggtgggt tgttggtgca ggtttggatg
     1141 atgttgccag aagtttactc tatgctatca tagtaatggg tatacttttt accggttggg
     1201 ggattggtat tttatttttt cttcttataa agaaaagaag taaaatacaa gacttgagtt
     1261 tttttagact attttttgct attaccctac tgtttttccc tgtaatattc gaacttttaa
     1321 ttctcaaaca atatttcata cttccattat cactttcatg cattattagt tcattggtta
     1381 tttcaataat aattcgtata tatggacgga tcttttctgt atcatgtttt tcagatatcc
     1441 cttttgttag agaacaccgc attaagttaa ttatggctgg ttttttggtt tatttttggc
     1501 ttttttcctt tcttgttggc tggtataaac ctcaattaaa aaaagaatat cagatgcttt
     1561 gttataataa tagttggtat tatattcttg ctcgttatga ttcacggctc gttttgtcta
     1621 gttcatttaa agatgacagt aacagattct taatatttaa tacagaacag tcgggatttt
     1681 atgaaatcaa tgatgtttat gtcagaaagt aataatcctc tcccacaaag tgaattaaat
     1741 gaatatataa tgcgttcgaa gtatatgtat tgagctgtca tactgagaaa caaaccttag
     1801 ggcagcacaa ttaaagccag aaacaactat gcaagaaagt tactaaataa gtgctcggaa
     1861 cagtaaaaca ctcagggggg actcagtccg gattatgtaa tcagataaat tgccaaacat
     1921 cacaaatcac cagccggact gagtgatgcc gagcattgca ccaatacccc gtgatgaacg
     1981 atgcctgatg cagaaagctc cctataaaac aaagaacaaa aactacgacc ggcgattcac
     2041 cgtcatactg atgctgcatc gtggtgacac tgtctgtcac tttcatgttg actgaacaac
     2101 tctgctgcga agaaggtgta ccgccggcgg gatttgattg tccggcgtct agcttccact
     2161 tccctgatgg aactgaaagt tgttatggcc ggtcagcttg ccctgtccgc aggacatgag
     2221 gtgctgtgac ggacagcacc agtaacggaa agtgacaatg aaaatgaata ctgcgctgat
     2281 agttgcacta atgtgtatgt ggtatgcggt acccgctgct gcaaaagaga ctttactggc
     2341 tatgccacgt aattcaacag aacattgcta tgcggagatt aatgtgcacg gaccatatgg
     2401 ggtgtatttc cgagtggtcc cgcatccccc cgggggaaag agctgggtcg aatgtaacag
     2461 tgactactat tactctgata aaccaccggg agtacagata ctcggaaccc gtgcagggtg
     2521 tcgggtatac ggaatctgcg ggaccacctc gacactgcat gtggctggaa gaggggttgt
     2581 gtgtattaaa aatatatgca gtccacgtgg gatgataatt cacaggatac ggaaacgtcc
     2641 tgtagtggct gtaagcgatg agatgtgatc ttacccagca atagtggaca cgcggctaag
     2701 tgagtaaact ctcagtcaga ggtgactcac atgacaaaaa cagtatcaac cagcaaaaaa
     2761 acacgtaaac agcattc
//
LOCUS       DAJTYZ010000274         2776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000274 DAJTYZ010000000
VERSION     DAJTYZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.279"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="OO596_004235"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="OO596_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..>2776
                     /gene="fimH"
                     /locus_tag="OO596_004236"
     CDS             1816..>2776
                     /gene="fimH"
                     /locus_tag="OO596_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCR6719897.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPT"
BASE COUNT          691 a          626 c          705 g          754 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccg
//
LOCUS       DAJTYZ010000275         2748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000275 DAJTYZ010000000
VERSION     DAJTYZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.280"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..714
                     /locus_tag="OO596_004237"
     CDS             1..714
                     /locus_tag="OO596_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6719898.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            746..1504
                     /locus_tag="OO596_004238"
     CDS             746..1504
                     /locus_tag="OO596_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719899.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1550..>2748
                     /locus_tag="OO596_004239"
     CDS             1550..>2748
                     /locus_tag="OO596_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6719900.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLL"
BASE COUNT          614 a          683 c          755 g          696 t
ORIGIN      
        1 gtgaatcaac gtaatatgtc aataattaat tccacgccag tgcgtgttat tgccattgta
       61 ggatgtgatg gttcaggtaa atcgcccctc acggcaagcc tggtaaatga actggcagca
      121 agaatgccaa cagaacacat ttatctcggg caatcgtccg ggctaattgg cgaatggatt
      181 tcacagctcc ctgttattgg cgcacctttt gggcgttatc tgcgaagtaa agcggcacat
      241 gtgcacgaaa aaccctcaac accgcctggc aatattactg ctctggttat ctatctgctt
      301 tcctgctggc gggcgtacaa gtttcgcaaa atgttgtgta aaagccagca aggctttctg
      361 ctcatcaccg accgctaccc gcaggttgaa gtgccgggtt ttcgctttga tggcccgcaa
      421 ttggcaaaaa ccacgggcgg taacggttgg ataaaaatgt taaggcagcg cgagctgaag
      481 ctgtaccaat ggatggcatc ttatttgccc gtattgttga ttcgtcttgg cattgatgaa
      541 caaaccgcgt ttgcgcgtaa acctgaccac caactggcag cgttacagga aaaaatcgcc
      601 gttacgccgc aactgacttt caacggcgcc aggatccttg aactggatgg tcggcaacca
      661 gccgatgaaa tcctgcaagc gtcactacgt gcaattcacg ccgcgctttc ctgatcacca
      721 aaatggatag gggatgattt aacgaatgga tgcactacaa acacaaactg ttaatagtac
      781 aaccgcgccg cagcccagct acattccggg gctaattgcg gtggtcgggt gcgatggcac
      841 cggtaaatcc acgctgacca ccgatctggt gaaatcattg caacagcact ggcaaaccga
      901 gcggcgctat ctggggctgc tctcgggcga agacggcgac aaaatcaaac gattaccgtt
      961 ggttggcgtc tggctggaac ggcgactggc ggccaaatcc tcgaaaaccc aaagcatgaa
     1021 aaccaaatct ccggcgctat gggcggcggt gattatgtac tgcttatcgc tgcgaagaat
     1081 ggcgaatcta cgcaaggttc agcgactggc gcaaagtggc gttctggtgg tcagcgatcg
     1141 cttcccgcag gctgaaattt cgggctttta ttacgacgga ccggggattg gcgtcgaacg
     1201 tgcgaccggg aaaatcagca tgtttctggc gcagcgcgaa cggcgtttat accaacaaat
     1261 ggcgcaatat cgcccggaat taattattcg cctgggcatt gatattgaga ctgccatctc
     1321 ccgcaagcct gaccatgact atgccgagct gcaggacaaa atcggtgtca tgtcgaagat
     1381 tggctataac ggcacaaaaa ttcttgagat tgattccaga gcgccctaca gcgaagtgct
     1441 ggaacaggca cagaaggcgg tttctctggt tgccatcgtt tctgaccgcc gaagtttaac
     1501 ttaaggctgg cgggatactt tttactatca acgataaggt caggctgaat tggcgggttt
     1561 taacatcaaa cattggtttg cagatggcgc gtttcgcacc attattcgca atagcgcctg
     1621 gttaggctcc agtaatgtcg tgagcgcctt gctggggctg ttggcgctct cgtgtgccgg
     1681 taaagggatg acgcccgcca tgtttggcgt actggtgatt gtgcaatcgt acgccaagtc
     1741 gatcagcgat tttattaagt ttcagacatg gcagctggtg gttcagtacg gaacgccagc
     1801 cttgaccaac aataatccgc agcaatttcg caatgtcgtc tcattttcct tctcgctgga
     1861 tatcgtcagc ggcgcggtgg cgattgtcgg cggcattgcc ttactgccat tcctttccca
     1921 ttcattaggt ctggatgacc aaagcttttg gctggcggcg ctgtattgca cgctcattcc
     1981 ttcaatggct tcctccacgc cgaccggcat tctgcgtgcg gtagatcgct tcgatttaat
     2041 tgctgtacag caggcgacga aaccttttct gcgcgcagcg gggagcgtcg tagcctggta
     2101 ttttgacttt ggttttgcgg gttttgttat tgcctggtac gtgtcgaatc tggttggcgg
     2161 caccatgtac tggtggtttg cagcgcgcga attacgccgc agaaatatcc ataatgcctt
     2221 caaattgaat ctgtttgagt ctgcccgaca catcaaaggc gcgtggagtt ttgtctggtc
     2281 aaccaacatt gcccactcca tctggtcggc gcgtaactcg tgcagcacag tgctggtggg
     2341 gatcgtgtta ggacccgctg ccgccgggtt atttaaaatc gccatgacat tctttgacgc
     2401 cgccggaacg ccagcgggtt tgctgggtaa aagtttttac ccagaggtga tgcgtttaga
     2461 tccgcgcacc accagaccgt ggttgttagg tgtgaagtcc ggtttactgg cgggcggaat
     2521 cggtattctg gtggcgctcg cggtgttgat cgccggcaag ccgctcattt cgctggtgtt
     2581 tggcgttaag tatctcgaag cgtatgacct gattcaggtg atgctgggcg caattgtgat
     2641 ttcgatgctg ggtttcccgc aggagtcatt gctattgatg gcgggaaaac agcgcgcttt
     2701 tctcgtggcg caaaccatcg cctcaatcgg ctacatcgta ttgctgtt
//
LOCUS       DAJTYZ010000276         2746 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000276 DAJTYZ010000000
VERSION     DAJTYZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.281"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>2746
                     /gene="lacZ"
                     /locus_tag="OO596_004240"
     CDS             <1..>2746
                     /gene="lacZ"
                     /locus_tag="OO596_004240"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCR6719901.1"
                     /translation="SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPI
                     TVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDS
                     RLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPSTQIS
                     DFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFGGEIIDE
                     RGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPL
                     WYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVII
                     WSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFP
                     AVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWV
                     DQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQF
                     RLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIELPELPQ
                     PESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQLTTSET
                     DFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSEATRIDP
                     NAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGS
                     GQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWD
                     L"
BASE COUNT          603 a          757 c          792 g          594 t
ORIGIN      
        1 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
       61 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg
      181 caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg
      241 tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtgacct
      301 atcccattac ggtcaatccg ccttttgttc ccgcggagaa tccgacgggt tgttactcgc
      361 tcacatttaa tattgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg
      421 gtgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac ggccaggaca
      481 gccgtttgcc gtctgaattt gacctgagcg catttttacg cgccggaaaa aaccgcctcg
      541 cggtgatggt gctgcgctgg agtgacggta gttatctgga agatcaggat atgtggcgaa
      601 tgagcggcat tttccgtgac gtctcgttgc tgcacaaacc gagcacacaa atcagcgatt
      661 tccatgttgc cactcacttt aatgatgatt tcagccgcgc ggtactggag gcagaagttc
      721 agatgtacgg cgagctgcgc gatgagctgc gggtgacggt ttctttgtgg cagggtgaaa
      781 cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt
      841 atgccgatcg cgtcacgcta cgtctgaacg tcgaaaaccc ggcgctgtgg agcgccgaaa
      901 tcccgaatct ctatcgtgcg gtggttgaac tgcacaccga cgacggcacg ctgattgaag
      961 cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg ctgctgctga
     1021 acggcaagcc gttgctgatt cgcggcgtta accgtcacga gcatcatcct ctgcatggtc
     1081 aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag aacaacttta
     1141 acgccgtgcg ttgttcgcat tatccgaacc atccgctgtg gtacacgctg tgcgaccgct
     1201 tcggcttgta cgtggtggat gaagccaata ttgaaaccca cggcatggtg ccaatgaatc
     1261 gtctgaccga tgatccgcgc tggttacccg cgatgagcga acgcgtaacg cgaatggtgc
     1321 agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgag tcaggccacg
     1381 gcgctaatca cgacgcactc tatcgctgga tcaaatctgt cgatccatcc cgcccggtgc
     1441 agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg atgtacgcgc
     1501 gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt
     1561 cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca
     1621 gtcttggcgg cttcgctaaa tactggcagg cgtttcgtca gtacccccgt ttacagggcg
     1681 gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaat ggcaatccgt
     1741 ggtcggctta cggcggtgat tttggcgata caccgaacga tcgccagttc tgcatgaacg
     1801 gtctggtctt tgccgaccgc acgccgcatc cagcgctgac agaagcaaaa caccagcagc
     1861 agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc
     1921 gtcatagcga taacgagctt ctgcactggt cggtggcgtt ggatggcaag ccgctggcaa
     1981 gcggtgaaat gcctctggat gttgctccac aagataaaca gttgattgaa ctgcctgaac
     2041 taccgcagcc ggagagcgcc ggacaactct ggcttacggt acacgtagtg caaccgaacg
     2101 cgaccgcatg gtcagaagcc ggacacatca gcgcctggca gcagtggcgt ctggcggaaa
     2161 acctcagcgt gacactcccc gccgcgcccc acgccatccc gcaactgacc accagcgaaa
     2221 cggatttttg catcgagctg ggtaataaac gttggcaatt taaccgccag tcaggctttc
     2281 tttcacagat gtggattggc gctgaaaaac aactgctgac gccgctgcgc gatcagttca
     2341 cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt gacccgaacg
     2401 cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt
     2461 gcacggcaga tacacttgcc gacgcggtgc tgattacgac cgctcacgcg tggcagcatc
     2521 agggaaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt ggtcaaatgg
     2581 cgattaccgt tgatgttgaa gtagcgagcg atacaccgca tccggctcgg attggcctga
     2641 cctgccagct ggcgcaggta gcagaacggg taaactggct cggattaggg ccgcaagaaa
     2701 actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatct
//
LOCUS       DAJTYZ010000277         2690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000277 DAJTYZ010000000
VERSION     DAJTYZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.282"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            230..769
                     /locus_tag="OO596_004241"
     CDS             230..769
                     /locus_tag="OO596_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6719902.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OO596_004242"
     CDS             complement(995..1318)
                     /locus_tag="OO596_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6719903.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OO596_004243"
     CDS             1604..1798
                     /locus_tag="OO596_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6719904.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAJTYZ010000278         2681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000278 DAJTYZ010000000
VERSION     DAJTYZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.283"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..997)
                     /gene="shiA"
                     /locus_tag="OO596_004244"
     CDS             complement(<1..997)
                     /gene="shiA"
                     /locus_tag="OO596_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCR6719905.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFP"
     gene            1312..2364
                     /locus_tag="OO596_004245"
     CDS             1312..2364
                     /locus_tag="OO596_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCR6719906.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          835 a          580 c          600 g          666 t
ORIGIN      
        1 aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta cggcgaccaa
       61 aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca cctaccagca
      121 aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa taattaagtg
      181 caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca ataatcttca
      241 ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt ttcgcggcag
      301 cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg cgcacccaca
      361 atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc cagcttaaaa
      421 actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa agcagtaaac
      481 ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt ttcggtgcac
      541 tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat ccctgaatga
      601 cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat gaaggaagaa
      661 taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac atgcgcttac
      721 gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg aaaaggaatc
      781 cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact tgcgggaaaa
      841 actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca taccagtcga
      901 cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac gaaagcgtcc
      961 cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt gtactgtctt
     1021 tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac gaaccttatg
     1081 agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg gcaacatgaa
     1141 aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat taagcagagg
     1201 acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg gcaccctgat
     1261 gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg tatgttttta
     1321 gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta taatttcaat
     1381 attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga tgctatggcc
     1441 atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt aattgtaacg
     1501 tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa attaacatta
     1561 gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt agcaaagaac
     1621 ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa aaagaacctg
     1681 aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt tgttggtata
     1741 accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca gaaactccgg
     1801 gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc agcagtcagg
     1861 ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga ggaaatgcga
     1921 tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg tacattttca
     1981 ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat accaatagtg
     2041 atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg ttataataat
     2101 gtctaccgtt tatccttacc accctcgatt aaacgttcag cagcaatcat aaaagatgct
     2161 tatatcgcaa taactcctga cacctcaata ttacatatgg caagtgccta taatacccct
     2221 gttgttgcaa tttatgctga ttacaaaacg cgatggcccg caatggccga tgtctcggag
     2281 tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg atgaattcgc aaaggcatta
     2341 aaaagtgttt tggcgagaat atgagacagt acttatgcat agtaatgccg atctgttatg
     2401 aacggtggtg tcatcaatac tctgaggctg gtcaatcatc acagaaaacc agcctttgaa
     2461 tcaaagcagt actcaccgga tacgatcata gtcacatcga acgcaattct ggcggtgccg
     2521 catttttatt tattcatttt ctgtgagaga agggcaagct ctatagaggg aatgtaaaaa
     2581 agagagctcc gaatggagca ctctttaaga aattataacg gaaaatcaga ggtttttata
     2641 acatgtcgca taagcaaacc caagcttttc aaatacgctt t
//
LOCUS       DAJTYZ010000279         2674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000279 DAJTYZ010000000
VERSION     DAJTYZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.284"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..424
                     /locus_tag="OO596_004246"
     CDS             <1..424
                     /locus_tag="OO596_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6719907.1"
                     /translation="FKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSAD
                     FLNTAKYPQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRA
                     GFEAEGKIKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(466..1518)
                     /locus_tag="OO596_004247"
     CDS             complement(466..1518)
                     /locus_tag="OO596_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6719908.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            1743..2663
                     /gene="lpxL"
                     /locus_tag="OO596_004248"
     CDS             1743..2663
                     /gene="lpxL"
                     /locus_tag="OO596_004248"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6719909.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
BASE COUNT          689 a          669 c          657 g          659 t
ORIGIN      
        1 ctttaaagat ttcgacggta cttttacctt tgacgaaaaa aatccggctg ccgataaagt
       61 gaatgtgaca attaacacca ccagcgtcga tactaatcac gccgaacgcg ataaacatct
      121 tcgcagtgca gatttcctca ataccgcaaa atatccacag gcaacattca cctccaccag
      181 cgtgaagaaa gacggtgacg aactggatat taccggcgat ctgacgctga atggcgtaac
      241 caaacccgtc acgctggaag cgaaattaat tggtcagggc gacgacccat ggggtggtaa
      301 acgtgcaggc ttcgaggccg aaggcaaaat taagctcaag gacttcaata tcaagacaga
      361 tttaggtcca gcttctcagg aagtggatct gattatttca gtggaaggcg tacagcagaa
      421 gtaagcgtga aatgttgccg gatggcacgc atccggcaat gatatttatt ctgttggatc
      481 aggaatgccc agtgttgtat tcagacgtcc acgagactta ttaaagatct tattgccatt
      541 ttcacgtcct gcccgacggc gtcgctgttc ttctggcggt aacgcgcttt cttcgcagca
      601 aatctcacta caacaacctt tgtatttttc cgcgcatact ggacactgaa taaacagcag
      661 gtggcagcca tcatttttac agttggtatg gctgtcgcac ggcgcgccgc actggtggca
      721 atgcgcgata atctcatccg atatacgttc gcccatccgc tcgtcaaaaa caaaattttt
      781 gccaataaaa cgcaccggca agccctgctc gcgcgcctta cgggcgtatt caataattcc
      841 gccctcgata tgccagactt tgttgaatcc gttatgtttc atccaggcac tggccttttc
      901 acaacgaatg ccgccggtgc agtacatgac gattttttta tctttatgtg cctgcatcat
      961 ctcgactgct tttggcagct gctcacggaa ggtatctgcc ggaatttcga gcgcgttttc
     1021 aaagtgcccc acttcatact catagtggtt acgcatgtcg ataaatagtg catcgggatc
     1081 gtcaagcatg gcgttcactt ccgccgcttg cagatactca ccaacattgc tggcatcaaa
     1141 gtgaggatcg tcaataccgt cggcaacaat gcgatcgcgt accttcatgc gcagtaccca
     1201 gaaggatttc ccgtcatcat ccaacgcgat attcaggcgt aaaccctcta aagccgggtc
     1261 gaaggcatag agctgcgcgc gaaatgtttc aacattgctc gcaggtacgc tgatttgcgc
     1321 gttaatgccc tcatgcgcca gatacactcg cccaaaaaca ttcagcgcgg taaacagctg
     1381 atataaagcg tcacgggtcg ccttaggatc ggcgatgtgg aaatacttgt aaaacgaaat
     1441 ggtggttcgc ggttcgctct caaccaacat tttggctttt aacgcgtcgt tggaaatgcg
     1501 gttgtgtaac actggcatgg tgtacggttc ctgcgagatg ggaaagtaaa aatccgcggc
     1561 atgatatagc aattatcgat aattaacatc cacacatttt acgctacatt tgcgcattaa
     1621 aaattatttg ttatttacaa gcgcggcaat ttcgcccagt cttcagccac aaattttggt
     1681 tgcgggcgaa aaaatgcgac aatacataca attgcccgaa taggttgaaa aacaggattg
     1741 atatgacgaa tctacccaag ttctccaccg cactgcttca tccgcgttat tggttaacct
     1801 ggttgggtat tggcgtactt tggttagtcg tgcaattgcc ctacccggtt atctaccgcc
     1861 tcggttgtgg attaggaaaa ctggcgttac gttttatgaa acgacgcgca aaaattgtgc
     1921 atcgcaacct ggaactgtgc ttcccggaaa tgagcgaaca agaacgccgt aaaatggtgg
     1981 tgaagaattt cgaatccgtt ggcatgggac tgatggaaac cggtatggcg tggttctggc
     2041 cggaccgccg aatcgcccgc tggacggaag tgatcggcat ggaacacatt cgtgacgtgc
     2101 aggcgcaaaa acgcggcatc ctgttagttg gcatccattt tctgacactg gaactgggcg
     2161 cgcggcagtt tggtattcag gaaccgggga ttggcgttta ccgcccgaac gataatccac
     2221 tgattgactg gctacaaacc tggggccgtt tgcgctcaaa taaatcgatg ctcgaccgca
     2281 aagatttaaa aggcatgatt aaagccctga aaaaaggcga agtggtctgg tacgcaccgg
     2341 atcatgatta cggcccgcgc tcaagcgttt tcgtcccgtt atttgccgtt gagcaggctg
     2401 cgaccacgac cggaacctgg atgctggcac ggatgtccgg cgcatgtctg gtgcccttcg
     2461 ttccacgccg taagccagat ggcaaagggt atcaattgat tatgctgccg ccagagtgtt
     2521 ctccgccact ggatgatgcc gaaactaccg ccgcgtggat gaacaaagtg gtcgaaaaat
     2581 gcatcatgat ggcaccagag cagtatatgt ggttacaccg tcgctttaaa acacgcccgg
     2641 aaggcgttcc ttcacgctat taaatctccc ctgc
//
LOCUS       DAJTYZ010000280         2556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000280 DAJTYZ010000000
VERSION     DAJTYZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.285"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..642)
                     /gene="curA"
                     /locus_tag="OO596_004249"
     CDS             complement(<1..642)
                     /gene="curA"
                     /locus_tag="OO596_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6719910.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKA"
     gene            823..1341
                     /gene="mddA"
                     /locus_tag="OO596_004250"
     CDS             823..1341
                     /gene="mddA"
                     /locus_tag="OO596_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6719911.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1338..1787
                     /locus_tag="OO596_004251"
     CDS             1338..1787
                     /locus_tag="OO596_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6719912.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1788..2021)
                     /locus_tag="OO596_004252"
     CDS             complement(1788..2021)
                     /locus_tag="OO596_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6719913.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2107..2280)
                     /gene="ortT"
                     /locus_tag="OO596_004253"
     CDS             complement(2107..2280)
                     /gene="ortT"
                     /locus_tag="OO596_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6719914.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2475..>2556
                     /gene="yncL"
                     /locus_tag="OO596_004254"
     CDS             2475..>2556
                     /gene="yncL"
                     /locus_tag="OO596_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6719915.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRF"
BASE COUNT          605 a          659 c          626 g          666 t
ORIGIN      
        1 cgctttcacc agttgttcgg caaaatcatc cgcgtggtga tcaagacaaa catcgaagcc
       61 cagcacctcg atagcatggc ggcatttttc cgcgccaccg gcaaccccca ccaccctgca
      121 acctttaagt ttgccgattt gccccaccgt tgctcccaca ggacctgtcg ccgcagctac
      181 caccaacgtt tcgccctctt taggctgacc gatatccagc agccccatat aagcggtaaa
      241 gcctggcatc cccagcacac ccagcgacca cgatggattt tgcggatgat cgccaagttt
      301 caccagatca tcaccactgg atatgtcata gtcctgccat ccactgtagc ccagcaccca
      361 gtcgctaggc tgataatcag gatgattcga ctccacaaca cggctcaccg tgccgccgac
      421 catcacgccg ccaatatcaa caggcggtga ataagatggc tcatcgctca tacgtccacg
      481 catatacggg tccagggaca aataaactgt gcgcagtaac acctgtcctt cacccggtgt
      541 ggcgacatca tcttcttcaa gacggaaatt ctccggaacc ggtgcgccat gtggacgcga
      601 ggccagaacc caacgtcgat tacgctgctt ttgttgcccc ataatgctct cctgtgcttt
      661 tggcatgaat ccatcaatag tagcaggctc acgggattgg ttttgtttgc agccgtcaca
      721 tcccctctgc aacacgcaat aatttcatcg aggaatccgt tgcttcgggc tgttttcgat
      781 agtattgata acatcatcat cattatttta tcgaggccag acatgtccat ccgttttgcc
      841 cgcaaagccg attgtgctgc cattgcggaa atttataacc acgccgtgtt gtatacggcg
      901 gctatctgga atgaccaaac ggttgatgca gataaccgca ttgcctggtt tgaagcgcgg
      961 accatagcag gttatccggt gctggtaagt gaagaagatg gcgtagtgac gggatatgcc
     1021 tcgtttggcg actggcgtag tttcgatggt tttcgccata ccgtggaaca ttcggtttat
     1081 gtccatcccg atcatcaggg caaaggtctg gggcgtaaat tgttaagccg attgattgat
     1141 gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga tcgaatcgca aaatcaggcc
     1201 tcgctgcatc tgcaccattc gctgggattt gtcgtcaccg cacaaatgcc gcaggtgggc
     1261 actaaatttg ggcgctggct ggatctgaca tttatgcagt tgcaactcga cgaacgcact
     1321 gaaccggacg cgattggatg aatcagtcgc tcacccttgc ttttttgatt gccgccggaa
     1381 ttggtctggt agtccagaac acgctgatgg tgcgcatcac ccagacctcc tctaccattc
     1441 tcatcgccat gttgctgaac tcactggtgg ggattgtgct ctttgtttct attttatggt
     1501 ttaaacaagg catggcgggg tttggcgaac tggtgtccag cgtgcgctgg tggacactta
     1561 tacccggctt gctgggatcg ttttttgtct tcgccagtat cagcggatat cagaatgtgg
     1621 gagcggcgac aactattgca gtactggtcg ccagccagct aattggcggt ctgatgctgg
     1681 atatcttccg cagtcatggc gtaccactac gggcgttgtt cggcccaatt tgtggcgcaa
     1741 ttttacttgt ggttggcgcc tggttggtag ccagacgctc attttaatca gaggatggtg
     1801 ccgcctttgg ttaactgttc gtggcgcgct tccatatctt ctttatgctt gcgaccgtga
     1861 tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc gatcttcccg acttaactcc
     1921 gcgtcatcgc tcagggcgat taataattcg ttcatatggg caatgacgct ctcttcaatc
     1981 gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca tactttctcc ttaaccactt
     2041 gtgaaattta tcgattcatc caaatgatat aagtctgcgt ggtgccgtca agccagtgag
     2101 taagatttaa aacagtgaaa acaacagcgc caccgggaaa ctcatcggcc atgttgcccc
     2161 caccagaaag gcgcttaaga aacggatgcg tttcttatcg tgagaaagaa accaggtgat
     2221 aagaaatgcg attgctgcca taaccgcata aaaaacaagc atatgttgat agagagacat
     2281 gtacgggcgt ccagccagat gaataaaacg cgcgcaaata tgcgtcattt tttgacgtgc
     2341 atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca gcaacttgcc cttatccatc
     2401 ttcagaatga gtattattgc ggattttctt aacccgtact atacacatag gggcaatgat
     2461 aaaaggtggc aaaaatgaat gtttccagta gaactgtagt actgataaat gtctttgctg
     2521 ctgttggttt gtttactctt atctctatga gatttg
//
LOCUS       DAJTYZ010000281         2502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000281 DAJTYZ010000000
VERSION     DAJTYZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.286"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..321
                     /locus_tag="OO596_004255"
     CDS             <1..321
                     /locus_tag="OO596_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCR6719916.1"
                     /translation="TKLLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQKYAIG
                     WSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIERI
                     ENYL"
     gene            318..797
                     /locus_tag="OO596_004256"
     CDS             318..797
                     /locus_tag="OO596_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCR6719917.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(851..1801)
                     /gene="corA"
                     /locus_tag="OO596_004257"
     CDS             complement(851..1801)
                     /gene="corA"
                     /locus_tag="OO596_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6719918.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2171..>2502
                     /locus_tag="OO596_004258"
     CDS             2171..>2502
                     /locus_tag="OO596_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719919.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          725 a          571 c          550 g          656 t
ORIGIN      
        1 accaaattac tttcatcaag actaaataaa tttaatgact tcgaggacag ttttaatggc
       61 ttaggaatca gtgttcatga tatttcagct caaaaaatat ctcttttaag ttttcaaaaa
      121 tacgctatag gatggagtgc aacaattcat tttgtagcac aggaccactt cggtcttgat
      181 gtaactgata tcaaaaacaa gacttacagc aaatatagat tttttcgcat atggttcttt
      241 ttacaacggc ataaagactt tgctttcaaa ccttttttca ccaattttaa cactatcgaa
      301 agaatcgaaa attatctatg aaaagcaaac tccagaaaat tatattatgt ttatttcttc
      361 tatgctgcat ctataattta tggacattgc gtcctgtaca gattttgtat acatactctg
      421 atgctggcaa ttcagtcttt ctggtcgttg atcatctgcc atggactgac agcgataaaa
      481 taaattggta tctgaagcat cagaatgaaa ttaaaaatca acatccccta ccagaaggta
      541 gttggcatac ctggtatgtc attgatatcg gtaatggctt cactgactat aaaaaatata
      601 tcgaagggcc gtatgaagat ctctactgtt tcccgacaat taagagcaat gataattgca
      661 tcgtgaaaaa ttacttaatg gttattaatg aataccctta tcgtaacact catattggta
      721 ttaacgattt tactgagtat caattaacac aagaaaataa aatagagcga gttttcaatc
      781 cgcatgactt taaataagac aatttttaga aataagccgg ataaaaaaat agctttatcc
      841 ggcactcata ttacagccag ttcttgcgct taaagtacag atacggtgcc aggcccgcga
      901 ggatcataaa gataatcgcg cctgggtagc cgaaactcca cttcagttct ggcataaact
      961 caaagttcat cccatagctg gaagcaacga gcgtcggcgg caggaatacc acggatacca
     1021 ccgagaagat tttgatgatg cggttctgct cgatgttgat aaaacccatc gccgcctgca
     1081 tcaggaagtt caccttctgg aacagggatt cgttatgcgg cagcagggat tcgatatctc
     1141 gcaggatttc acgcgcctgc tccagttgcc cacccggtaa acgcgcttta cgcaccagga
     1201 agttgagcgc gcgctgggta tccatcagac acaggcgaac tttccagccg atatcttcca
     1261 gttccgccag agtggagagc gcctcgtcgt actcatcgcc ctgatgccct tccataatca
     1321 cccggctcaa ctgctccagg tcgctataga tattttcaat ttcatctgcc aactgttcga
     1381 ttttggtttc gaacagatcc agcagcagct cgtaggcgtt accgtctacc atcgactggc
     1441 tacgggcacg catacgatac agacgaaaag cgggcagttc acgctcacgc agagtaaaca
     1501 gacgaccatc acggatggta aatgccacag tggagttacc ggcgtgatct tccgcatctt
     1561 caaagaagaa gaaggagtga atatgcaggc cgtcgtcgtc ttcaaagaaa cgtgccgatg
     1621 cttcgatgtc ttccagttcc gggcgggttg ccaggctctg gccaagttca gattgtacgc
     1681 gcagtcgctc gtcgtcgtcc ggttcgacaa gatcaatcca tactgcattt acaaggggtt
     1741 gtgactcttc gacttccagc cgggtcagtc ggttattttc cagttgaaat gcgctcagca
     1801 tgaccgggac tcccaatgct taaaatatcg gacagttcgg tgggcacaca gaaacaaatt
     1861 gggtttcaga ccattaaaca gcctgactca gcgcgacggg aaaaattgag gtcgctgaca
     1921 accgctaagg ctatcagcaa aaagggatag ccttaggagt tgatcctgga tgacaggata
     1981 gtgagccagt atctactggg tgtgtccaag gcgaatgtcc tcttagagtg atcgtgcgcg
     2041 catgttacgc cagcaaaatt ttgccgtcaa cacgcaacag aacaccacag aacaatagtt
     2101 gaacttcgcg ctttttggta ctcgtcaagg tcgctaacag gataaaaaaa tccaacatcc
     2161 aggacctata atggcgcatc ggctactcat tggtaaagga atgatcacct tgaatctcaa
     2221 acggattttt ctcgccctca ccttacttcc cttatttgct gttgccgctg atgattgcgc
     2281 actctcagat ccgatgctga ccgtacaggc gtataccgtt aatcctcaaa cagagcgggt
     2341 gaaaatgtac tggcaaaaag ccaatggcga agcgtgggga actttacatg ctctgctggt
     2401 ggatatgaat agtcagggtc aggtgcagat ggcgatgaac ggcggcatct atgatgaaag
     2461 ctatgcgccg ctcggtttgt acatcgaaaa cggtcagcag aa
//
LOCUS       DAJTYZ010000282         2463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000282 DAJTYZ010000000
VERSION     DAJTYZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.287"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..423
                     /gene="mscS"
                     /locus_tag="OO596_004259"
     CDS             <1..423
                     /gene="mscS"
                     /locus_tag="OO596_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6719920.1"
                     /translation="SVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSREPVRRNEFII
                     GVAYDSDIDQVKQILTNIIQSEDRILKDREMTVRLNELGASSINFVVRVWSNSGDLQN
                     VYWDVLERIKREFDAAGISFPYPQMDVNFKRVKEDKAA"
     gene            562..1197
                     /gene="argO"
                     /locus_tag="OO596_004260"
     CDS             562..1197
                     /gene="argO"
                     /locus_tag="OO596_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6719921.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            1290..2030
                     /locus_tag="OO596_004261"
     CDS             1290..2030
                     /locus_tag="OO596_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6719922.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            2197..>2463
                     /locus_tag="OO596_004262"
     CDS             2197..>2463
                     /locus_tag="OO596_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6719923.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSG"
BASE COUNT          623 a          560 c          627 g          653 t
ORIGIN      
        1 agtgtgcaga ttttctccac caccatgcgt actgcagacg gtaaaattat cgttattccg
       61 aacggtaaaa ttattgccgg aaatattatt aacttctctc gcgagccagt tcgccgtaac
      121 gaatttatta ttggcgtggc gtatgattcc gatatcgatc aggttaagca gatcctgacc
      181 aatattatcc agtctgaaga tcgcattttg aaagatcgcg aaatgactgt gcgtctgaac
      241 gaacttggtg catcgtcgat taatttcgtg gtccgcgtct ggagcaacag cggcgatctg
      301 caaaacgtgt actgggatgt gctggagcgt attaaacgtg aatttgatgc cgccggtatc
      361 agcttcccgt acccgcaaat ggatgtgaac tttaagcggg tgaaagaaga caaagctgcg
      421 taatcaacgc tgaggccaga taatactctc tggcctctct cttattagtt attctgattg
      481 ccaattaata ttatcaattt ccgctaataa caatcccgcg atatagtctc tgcatcagat
      541 acttaattcg gaatatccaa cgtgttttct tattactttc aaggtattgc tcttggggca
      601 gctatgattc tgccgctcgg tccacaaaat gcttttgtga tgaatcaggg cattcgtcgt
      661 cagtaccaca ttatgattgc cttactttgt gctatcagcg atttggtcct gatttgcgcc
      721 gggatttttg gtggcagcgc gttattgatg cagtcgccgt ggttgctggc gctggtcacc
      781 tggggcggcg tagccttctt gctgtggtat ggttttggcg cttttaaaac agcgatgagt
      841 agtaatattg agttagctag tgccgaagtc ctgaagcaag gcagatggaa aattatcgcc
      901 accatgctgg cagtgacctg gctgaatccg catgtttacc tggatacttt tgttgtactg
      961 ggcagccttg gcgggcagct tgacgtcgaa ccaaaacgtt ggtttgcgct cgggacaatt
     1021 agcgcctctt tcctgtggtt ctttggtctg gctattctcg cagcctggct ggcaccgcgc
     1081 ctgcgcacgg caaaatcaca gcgcattatc aatctggtag tgggatgtgt catgtggttt
     1141 atagccttgc agctggcgag agacggtatt gctcatgcac aagccttgtt cagttaggcg
     1201 ctatctgatg gaaaaataaa acagaggcgc taagcttgcc tccagaggtc ctgaattttg
     1261 ggcaagtcaa ttaacacgga gagactaacg tgaagttcaa agttatcgcc ctggcggcat
     1321 taatgggtat tagcgggatg gcagcgcagg ctaacgaatt gccggatgga ccgcatattg
     1381 tcacctccgg tacggcaagt gtggatgcgg tgccagacat tgccactctt gcgattgaag
     1441 ttaacgtggc cgcgaaggat gccgctacag ccaagaaaca ggcagatgag cgcgtcgcac
     1501 aatacatttc cttccttgaa ctcaatcaga tcgcgaaaaa agatatcagc tcagcgaact
     1561 tacgcaccca gccagattat gattatcagg atggtaaaag tatcctgaaa ggctaccgcg
     1621 ctgtgagaac ggtggaagtc acgctccgtc agttagacaa actgaattcc ttgctggatg
     1681 gcgcgctgaa ggcgggtctt aacgaaattc gttctgtgtc gctgggcgtg gcgcagccgg
     1741 atgcctataa agacaaagcg cgtaaggcag cgattgataa cgcgattcat caggcgcagg
     1801 aactggcgaa cggctttcat cgtaaactgg ggccggtata tagcgtgcgc taccatgttt
     1861 ccaactatca gcccagccca atggtgcgga tgatgaaagc cgatgccgcg ccggtgtctg
     1921 cccaggaaac ttacgagcag gccgctattc agtttgatga tcaggtcgat gtggtcttcc
     1981 agttagaacc tgtggatcaa caacccgcta aaacacctgc agcacaataa tacttgtagc
     2041 ccgacgtatt cgtcgggcta atgcctttac ccgatattgc ctcctttttc tgtaattttc
     2101 tgtaaaacca tgacgtaatc acctatcttt gctcgcggtt tcatcaagga taggctgact
     2161 gaaacggtta agcggacaag gatgcgggga ggggggatgg acatttttat ttcgaaaaag
     2221 atgcgcaatt tcattttatt agcgcaaacc aataatattg cccgggcggc agaaaaaatc
     2281 catatgacgg cttcgccatt tggtaaaagc attgccgcgc tggaagaaca aattggctat
     2341 acgctattta cccgcaaaga taataatatc agcctcaata aagccggtca ggaactgtat
     2401 caaaaactgt tcccggttta tcaacgactt tctgcgattg ataatgaaat ccataactca
     2461 ggg
//
LOCUS       DAJTYZ010000283         2446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000283 DAJTYZ010000000
VERSION     DAJTYZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.288"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(25..315)
                     /locus_tag="OO596_004263"
     CDS             complement(25..315)
                     /locus_tag="OO596_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6719924.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="OO596_004264"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="OO596_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6719925.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="OO596_004265"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="OO596_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6719926.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="OO596_004266"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="OO596_004266"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2446)
                     /locus_tag="OO596_004267"
     CDS             complement(2171..>2446)
                     /locus_tag="OO596_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6719927.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          667 a          655 c          549 g          575 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattccagat acgacc
//
LOCUS       DAJTYZ010000284         2436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000284 DAJTYZ010000000
VERSION     DAJTYZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.289"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(108..1526)
                     /gene="phrB"
                     /locus_tag="OO596_004268"
     CDS             complement(108..1526)
                     /gene="phrB"
                     /locus_tag="OO596_004268"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6719928.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1523..2032)
                     /locus_tag="OO596_004269"
     CDS             complement(1523..2032)
                     /locus_tag="OO596_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6719929.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          617 a          628 c          557 g          634 t
ORIGIN      
        1 tcaggcctac gtatttcctg caatttattg aatttgcaca aatttgtagg ctggataagg
       61 cgttcacgcc gcatccggca tctgggctcg attgcctgac gcgtctgtta tttccccttc
      121 cgcgccgcct catacgctgc caacgtttgt actctcgcct ctttgtgctc gactatcggt
      181 tgcggataat ccagcttcac acctgctttc tgcgcccact tccacggctc atgcaccgat
      241 ttccccggta cattgcgtag ttccggtagc cactggcgga taaactcacc ttcgagatca
      301 aatttctcgc cctgggttgt cgggttgaaa atacgaaaat acggcgctgc atcggttccg
      361 gttgaagcgg cccactgcca gccaccgtta ttggctgcca aatcaccatc aatcagctgc
      421 gacatgaaat atcgctcgcc ttcgcgccag tcgatcaata aatctttcac cagaaaactg
      481 gctgtaatca tccgtagcct gttatgcatc cagccagtgc tgttaagctg acgcatagcg
      541 gcatcaacaa tcgggtatcc cgttttgcct tcctgccagg cctgtaaatg tgcgggattg
      601 ctctgccact gtacacgatc cgtccaggca ataattggac gatgtttaca caacgagggg
      661 tgatacgtta tcaggtgacg gtaaaactcg cgccagatca gctcattaag ccagacacta
      721 ccgaccccac cgtccagcgc ctgcggctgt tcagccaaca agcgatgcaa gcactggcga
      781 ggcgataacc cgcccgttgc caggctggcc gacaaacggc tggtgccttc cactgccgga
      841 aaatctcgtt gttgctcata ttctccggca ccgttctggc aaaactggcg taattgcgca
      901 atcgccgctt tttcttccac cggaaaatgc gcagtatcga aagactgacg aggataattc
      961 agcgtaatgg atggcgaggg ctctatcgat ccgctactac gaacttttgg cgcagcgacg
     1021 cactccggca tcccttcccg cagccgtttc agccaggcat tcttaaaagg cgtaaagact
     1081 ttgtacatct cgtgattacc ggtcatcacc gcgccaggcg gcaggatcac gctgtcatca
     1141 aatccttcac acaccacgtt acgcagcgct ctttcaactt ccacatcccg cgcccgctca
     1201 ttcacttcat actgatagtt ataaaacagg tgggtaacgc tgttttccgc gcacacctgt
     1261 ttaactattt cgacactggc gacaaagtca tccacttcac ggaacaataa aggaatacct
     1321 ttttccgcaa gcgctatttg tagcccattc agttgagcat tgataagttc agcctgacgc
     1381 ggcgacatgt tatgcgtcgc ccactggcgt ggtgtagcga tatacaacgc cagcacgcgt
     1441 gcagacgaat tgcggcaggc ggcagccagt gcgagattat cgtgcagacg taaatcttgg
     1501 cgaaaccaga ccagatgggt agtcataaaa ctcctgaatg gcgcaagtta atcacacgcg
     1561 gccaaagttc gaaataacgc tgccctgaaa gccaggcgtc atgataaagc gccatatagt
     1621 gtttgatacg catcagcggc gcaagaagtg gttgctcgcc acggcgataa ctgtcgataa
     1681 gcgcagccag ctgctggcgt tctgtggaat caatatgcgg gcgaaaataa ccctgaacgt
     1741 gcatcaaaac attggtgtga tcgcgcgggt tggcggggtg agaaagcaaa acaactacgc
     1801 gttggcggta ctggttgtaa tagtcatcca gattttgcca ctggtgaata tcagccacga
     1861 acgggccaag tttgcggtac tccggctgag aatgcgccag aaagacgagt ttatagcggc
     1921 tgtgataatc gagaagcgcc ccgcgcgtta agccatgttc tttcagtcga tgtaactcat
     1981 gaagggcaaa gatacggatt agggtgctgt catcaaatcg ttgtagattc attactgctc
     2041 ctgaacggta taaaggagta agtgtagacg atggtaaatg aattttatgg ggcagagttt
     2101 ttgcggggaa gtattttgtt ggtaaaaata ttatcaactt catctgagtg agaattattc
     2161 tactatttcc cgagctgcat tcattttttg tattttttat ttttagaaaa taacagttct
     2221 caaagcttaa ttacctcact attaattgta tttcaaacta acaatcgcac tccattttct
     2281 ctttttaaaa aacataaaca ctgttaaaac aaatacttat ttcgttatct taaatgctat
     2341 ttctacatca ctcgtcctta taatcaaatt attcttattc atagctattt ttgcaggttt
     2401 aaaatagcgg ctttcaccaa agtcagccat gaaaat
//
LOCUS       DAJTYZ010000285         2367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000285 DAJTYZ010000000
VERSION     DAJTYZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.290"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            53..1006
                     /locus_tag="OO596_004270"
     CDS             53..1006
                     /locus_tag="OO596_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6719930.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1047..2042)
                     /gene="pgl"
                     /locus_tag="OO596_004271"
     CDS             complement(1047..2042)
                     /gene="pgl"
                     /locus_tag="OO596_004271"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6719931.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          642 a          599 c          544 g          582 t
ORIGIN      
        1 atttttaacg gccagccacc caaaatcgtc gcttccattt tccggagtaa taatgaagca
       61 tgaattatca agtatgaagg catttgtcat actggcagag tccagttcgt ttaacaatgc
      121 cgctaaatta ctcaatatta cgcaacctgc attaacgcga agaataaaaa agatggagga
      181 agatttacat attcaactat ttgagcgtac aactcgcaag gttacgttaa caaaagcagg
      241 aaaaaggttg ctcccggagg cgcgggaatt aataaagaaa ttcgatgaaa cgctttttaa
      301 tattcgtgat atgaatgctt atcatcgtgg tatggtgacg ttagcgtgta ttccgaccgc
      361 agtattttat tttttaccgc tggcaatcgg taaatttaat gagctgtatc ccaatattaa
      421 agtgcggatt ctggaacaag gcacgaataa ttgcatggaa tcggtgctgt gtaacgaatc
      481 agactttggc ataaacatga acaacgtcac aaattcatcc attgatttta ccccactggt
      541 caacgagccg ttcgtgctgg cctgtcggcg tgaccatcca ttggccaaaa agcagcttgt
      601 agaatggcag gagttagtgg gttacaaaat gattggcgta cgctcctcca gcggcaaccg
      661 actgttgata gaacaacagc tggccgacaa gccctggaag ctggactggt tttacgaagt
      721 gcgtcatctt tcgacgtcat taggactggt agaggcggga ctggggattt cggcgcttcc
      781 tggtctggca atgccccatg cgccctattc ttcgattatt ggtattccac tggtggaacc
      841 cgttatacgt cggacattgg ggattattcg ccgcaaagat gccgtgcttt ctccggcagc
      901 agaacgcttt tttgccttgc tgattaattt gtggactgac gataaagaca atttgtggac
      961 caatatcgtc gaacgccaga gacatgcgtt acaggagata ggttaataca ccggcgcagg
     1021 agagactgcg ccgggtaaat cagcggttag tgtgcgttaa ccaccaccca cattggtccc
     1081 tgcccgaccg catagcggcc tttttcatgc agtagcccct gctcgccaac aatttcgtat
     1141 accgagatgt ggtgagattt ttgcccggcg acaatcagat acttgccgct gtgatcaaca
     1201 ttgaagccgc gcggctgggt ttccgttggc tggaagcctt ctttactcaa cacgctgcca
     1261 tcttccgaaa cgctgaaaac ggtaatcaga ctggcggtac ggtcgcaggc gtataaatga
     1321 cgaccatccg gggtgatatg aatatcagcc gcccaacagg tgtcggagaa gttttccggc
     1381 atcatatcca gcgtctggac acattcaata ttgccgtgtg gatctttcag ttcccagaca
     1441 tccactgagc tgtttaactc attgacgcaa tacgcatatt gttcgtttgg atggaatacc
     1501 atatgacgcg ggccggcccc ttcaacggta gtcacttccg cagggtcctg cgccaccaga
     1561 tgaccatcat cgctgaccgt aaacaggcaa atgcgatcct gctttaatgc cggaacccac
     1621 agcgtacggt tgtccggtga gatattggcg gaatggcaac cgtccagccc ctcgaccaca
     1681 tcgacgacgc ccactggcag gccatcttcc agacgcgtta cgctcacgtt acccgcattg
     1741 taagaaccta caaagacaaa ctgcccctgg tgatcggtgg aaatatgcgt cggactaccc
     1801 ggcagcgcag actctgcggc aaaggtcagt gcgccatcgt ccggggcaat acgatacgcc
     1861 aggacgcgaa actcagggcg aacaccaaca tagagataac gtttgtccgg gctgaccacc
     1921 atcggctgca cctgccccgg cacatcgaca acctgtgtca gcgtcagtgc gccttcatga
     1981 ttcaggttcc agacgtgaat ttgctggctc tcagggctgg cgatataaac tgtttgcttc
     2041 atgaacacac ctcaaaaatg ataaatccct tatctttcat acacataaca acacttactg
     2101 gataaaaaac agacaacaaa acacgataaa atacaatatc gcacatcaat taaatcaata
     2161 agttagcact ccatttcgaa tggcatatta ctaaaatatt agtcaccgac acgaatttga
     2221 caatgccaca gatattttcg cgactattca gggagtatga ctctccccgc ttttgtttac
     2281 ctgtatcaac gaccgtaaac actatgccat aacaacttct ggccaacttc ccattcgctc
     2341 aacaatcaac tcacttccgg cacaaat
//
LOCUS       DAJTYZ010000286         2314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000286 DAJTYZ010000000
VERSION     DAJTYZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.291"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2139
                     /locus_tag="OO596_004272"
     CDS             <1..2139
                     /locus_tag="OO596_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6719932.1"
                     /translation="KDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQIALYRDARGY
                     VSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPWRLRNYSTWTR
                     NDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLASDDNMLPNSQ
                     RGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSGDLEVTIEESD
                     GTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEATAIYGLNNTFT
                     LYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGYQWRTQYIKDI
                     PETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTIFDGVSLYASG
                     SQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSLNLSIPLERWL
                     PRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHNSSLNASYRSP
                     YGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDANGASGVRIQNY
                     PGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNA
                     NIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISSKSQSWTVRWG
                     NQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2150..>2314
                     /locus_tag="OO596_004273"
     CDS             2150..>2314
                     /locus_tag="OO596_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6719933.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          640 a          561 c          544 g          569 t
ORIGIN      
        1 aaagatgacg atagcgtgat caactcgctt gaacaaatca ttcccggtac agcagctgaa
       61 tttgatttca atcatcagcg acttaatttg agcattcccc aaattgcact gtaccgtgat
      121 gcaagaggtt acgtctcccc ttctcgttgg gacgatggta taccaacgct gtttaccaac
      181 tactcgttta caggttctga taaccgttac cgccagggca atcgtagcca acgacagtac
      241 ctgaatatgc aaaatggtgc taattttggc ccctggcgat tacgcaacta ttccacatgg
      301 acacgcaacg atcagacatc aagctggaat accatcagta gttatttaca acgtgatatc
      361 aaggcattga agtctcagtt gcttctggga gaaagcgcca ccagcggcag tattttttcc
      421 agctacacct ttactggcgt tcaactcgct tccgacgata atatgttgcc aaacagccag
      481 cgcggatttg ccccaacggt acgcggtatc gcaaacagta gtgcaatcgt gactatcagg
      541 caaaatggtt atgtgatcta tcaaagcaac gtgccagcgg gtgcctttga aattaacgat
      601 ctctacccct cttccaacag cggcgattta gaagtcacga ttgaagaaag tgacggtacg
      661 caacgtcgct ttatccagcc ttattcttca ttacccatga tgcagcgacc tgggcatcta
      721 aaatatagcg cgaccgctgg acgctatcgc gctgatgcaa acagtgatag caaagaaccc
      781 gaatttgctg aagccacggc aatatatggt ttgaataata cttttacgct gtatggtggc
      841 ctgctcggtt ctgaagatta ttatgcgctg gggatcggta tcggcggcac acttggcgca
      901 ctgggcgcgt tgtcgatgga tatcaacaga gctgacaccc aattcgataa ccagcactct
      961 tttcatggct atcaatggcg tacgcagtac atcaaagata tcccggaaac caacaccaat
     1021 atcgctgtca gctactatcg ctataccaac gatggctatt ttagttttga tgaagccaat
     1081 acccgtaatt ggaactataa cagtcgccaa aaaagtgaaa ttcaattcaa catcagccag
     1141 acaatatttg atggggtaag tctgtatgcc tccggttcgc agcaagacta ttggggcaat
     1201 aacgataaaa acaggaatat ctcagttggg gtttccggtc agcaatgggg aattggttac
     1261 agcctgaatt atcaatacag tcgctacacg gatcaaaata atgaccgcgc actctctttg
     1321 aatctcagta ttccgttaga acgctggtta ccgcgtagcc gggtttccta tcagatgacc
     1381 agccagaaag atcgcccaac ccaacatgaa atgcgtctta atggctcact gctggatgat
     1441 ggtcgcctga gctatagcct ggaacaaagt ctggatgacg ataacaacca taacagtagc
     1501 ctgaacgcca gttaccgttc accttatggc accttcagtg ccggatacag ctacggtaat
     1561 gacagcagcc aatacaatta cggcgttacc ggcggcgtgg ttatccatac tcatggcgtg
     1621 acgctctcgc aatatctggg caacgctttt gcgcttatcg atgctaatgg ggcttctggc
     1681 gtgaggatac aaaactatcc ggggattgct actgatccct ttggctatgc agtggttcct
     1741 tatctcacga cttaccagga aaaccgtctc tcggtagata ctacgcagct gcccgataac
     1801 gtcgatcttg agcaaacaac acagtttgtg gtgcccaaca gaggtgcaat ggtagcggcg
     1861 cgtttcaacg ccaatatcgg ttatcgcgta cttgttatag tcagcgatcg caacggtaaa
     1921 ccgttgccct ttggcgctct tgccagcaac gatgagacgg ggcaacaaag tatcgtcgat
     1981 gagggcggca tactatatct ctctgggata tcgagtaaat cacaaagctg gactgtacgc
     2041 tggggaaatc aggcagatca acaatgtcag tttgctttta gtacaccgga ttcagaacca
     2101 acaacctctg tattacaagg cacggcgcag tgccattaag gataaaaaaa tgaaaagagc
     2161 gcctcttata acaggacttt tgttgatatc cacatcctgc gcttatgcct cctcaggagg
     2221 gtgtggagct gacagcacta gcggtgcgac aaattacagc agtgtggttg atgatgttac
     2281 ggtgaaccag acagataacg tgacaggacg ggag
//
LOCUS       DAJTYZ010000287         2293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000287 DAJTYZ010000000
VERSION     DAJTYZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.292"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1123..1458)
                     /locus_tag="OO596_004274"
     CDS             complement(1123..1458)
                     /locus_tag="OO596_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HCR6719934.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1587..1934
                     /locus_tag="OO596_004275"
     CDS             1587..1934
                     /locus_tag="OO596_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719935.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1954..2292)
                     /locus_tag="OO596_004276"
     CDS             complement(1954..2292)
                     /locus_tag="OO596_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001455284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719936.1"
                     /translation="MAQPLLLAEQDRIRAEQRAAEVVVLPLVPAAGLPAGGTSATDLA
                     VELNGITYQACRGDFVVRLDGSTCLQLWNKEGWVVRREGDPLEVAQWLPACHDAGMEV
                     RVQINESAAP"
BASE COUNT          696 a          505 c          437 g          655 t
ORIGIN      
        1 tatagtcaca taaaccaccg attgagtgac agttactgaa acaactattt cgacacaaaa
       61 tctcgtctat tcaacatcat gcaaatttgt cagtgactga atttagtgac agaaccctct
      121 ggaattctgc cgtgtaacca cttatatgca gacctgcccc gttgctctgg gttttcaggc
      181 tgcgatgagt gatgagcgat gatttccata cagatgatat atcgactgga attgattgcc
      241 tgcaattaga tttagtttta ttgtgtggat cgttttagtt agtggggatg aatttccgat
      301 aaattaacca atggaaagga gatgatatga ataaaaagat aactggatta ttaataatta
      361 gtctggcgtc aattttctta attgattgca cccagtgcca ttaacatcta tctacacaaa
      421 aatagaggaa aacaattttg atatatcaaa taattacaag cagctccatt ctttttattg
      481 tttgggggat tacatctata ataattagaa caaagaatta tttttctaag aagaaaacag
      541 accttaatat aagctttttt atagccttcc ctatttctat aacaatcctt ttattttgcc
      601 gaggtatatt ataaatataa cataatacat ctatatgggg tgtacatatc cccacataaa
      661 aacacatact ctcattaaca cgggaagaaa ctatgttttc caattctcac tacatgtaca
      721 taatcgttct tgtactatgg tttagtattc tcaagtactt acagagaaag aatagcatat
      781 tacatccttt attttcacta atcctgacac cactaaccat tttaatcatt agacttatga
      841 tagatttaac atacaaccaa taaattataa acaaaataaa aatagggaac attatatgct
      901 ttataaaatt ttcacattaa acacaaagat aaaaacaact atcattacaa tatgtgcatt
      961 atcatataat cttacagcgc aagcttcccc gtcatacaga cctcgtgatc gagttaataa
     1021 gactatgata gaactacaaa ctaacggagt agaacggcaa cttttagata attcttgtgg
     1081 cataagttct ggggtattta aataaaatat aagacaggct gtctatctta cagacagcct
     1141 ttttataata acagaaagaa tatatatcat acttaacgga agagataata tgaaaacagt
     1201 acccgtataa acagcaagca atcccatttt tccaggtgta tcagcaaaaa aaacgctgtt
     1261 ccagaaatca ggccgggtaa atttcagagc tgtatcttca atataccatt ttgcaaccgg
     1321 atacaaaacc attccgcaga gagaaataca ccagaacagt aatctgtatt taaaatcata
     1381 atcccatgac atatacagca tatatcctcc cgtcacccat ccccaccaca tattattaaa
     1441 ataatatttt ctgttcatct gccagttttc ctttcttttg cacttttgtc agtgtactga
     1501 tgcatgacaa cataaccaca gcatgataat aataatcaat aacaataagc tgtgtcacgt
     1561 ttacatcatg gaacggaggt gctcctgtga cttttgagca gaaaaaagcc cgggccattg
     1621 ccctgatgga cagcaaaaag atgtggcgga gcaattatgc ccctcctctt ctgcgtattc
     1681 tctggcggct gggtatccgg cttccgccat taccctttat gccgttctgg caggtcacgg
     1741 tgctgacggg gggcttatgg ggcatttcct ggggatgtgc aatgtggttt atctactggg
     1801 ggccgtcagg aatggtggca ggtgaggcca ttattataag tatcacgggg ggttttttgt
     1861 ccggactgct catggcctct ttccactggt ggcgcaggaa agttaaccga ctgccaccat
     1921 gggatgatgt gtaaaccgtg cacaggcgtg ttcttacgga gcggcacttt catttatctg
     1981 tacgcgaacc tccattcctg catcgtgaca ggccggcagc cactgcgcca cttccagcgg
     2041 atcgccctcc cggcgtacca cccagccttc tttattccat aactgcagac aggtgctgcc
     2101 gtcgagacgc accacaaaat ccccacggca ggcctgatac gttatgccgt tcagctccac
     2161 cgccagatca gtggcagaag tgccgcctgc gggcagacct gctgccggga ccagcggcag
     2221 tacgacgact tctgccgcac gctgttctgc ccggatgcgg tcctgttctg ccagcagcag
     2281 aggctgtgcc acg
//
LOCUS       DAJTYZ010000288         2255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000288 DAJTYZ010000000
VERSION     DAJTYZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.293"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            228..494
                     /gene="hipB"
                     /locus_tag="OO596_004277"
     CDS             228..494
                     /gene="hipB"
                     /locus_tag="OO596_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCR6719937.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            494..1815
                     /gene="hipA"
                     /locus_tag="OO596_004278"
                     /pseudo
     CDS             494..1815
                     /gene="hipA"
                     /locus_tag="OO596_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            1999..2100
                     /locus_tag="OO596_004279"
                     /pseudo
     CDS             1999..2100
                     /locus_tag="OO596_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          632 a          475 c          565 g          583 t
ORIGIN      
        1 tgaaatatag ctggtaaacc gtataagccg catgtcgaga tggcatgcgg cttaatattg
       61 ccaacttcaa aaggctttta aagcctccct tttatccgcg atcgcggata tcgcagcgtt
      121 tatcccgtac agcggataag atgtgtttcc agattgactt tatcctcact aaaggataaa
      181 actgataata tccccttaag cggataaatt tgctgtggac gtatgacatg atgagctttc
      241 agaagatcta tagcccaacg caattggcga atgcaatgaa actggttcgc cagcaaaatg
      301 gctggacgca gagcgagctg gcgaaaaaaa ttggcattaa gcaggcgacg atttccaatt
      361 tcgaaaacaa ccctgacaat accacgctga cgaccttttt taagatttta cagtcgcttg
      421 aactctcaat gacgctatgc gacgcgaaaa atgcctcgcc agaagcaaca gaacagcaag
      481 atctggagtg gtaatgccta aacttgtcac ttggatgaac aaccagcggg taggcgagtt
      541 aacgaagtta gccaacggcg cgcacacctt taagtatgca ccggagtggt tagccagccg
      601 ttatgccaga ccgttgtcac tttcgctgcc attgcagagg gggaatatca cctctgatgc
      661 cgtatttaac ttcttcgata acctgttacc cgatagcccg attgtacgtg accggatcgt
      721 taaacgttat catgccaaat ccagacaacc gtttgatttg ttgtcagaaa tagggcgaga
      781 cagcgttggt gccgtgacgt taatacccga agacgaaacc gtaacgcgtc cgataatggc
      841 atgggaaaag cttactgaag ccagacttga agaagtatta acggcttata aagcagatat
      901 cccgctaggc atgattagag aagaaaatga ctttcgcatc tcggttgctg gcgcacagga
      961 gaagacagca ctgctcagaa taggcaatga ctggtgcatt ccgaaaggaa taacgccgac
     1021 gacgcacatc attaaattac cgattggcga aatcaggcag tccaatgcga cgctcgatct
     1081 cagccaaagc gttgataatg agtattactg tctgctgctg gcgaaagaac ttgggttgaa
     1141 tgttccggac gcagaaatca ttaaagcggg aagggtgcgc gcgttagcgg tcgaacgttt
     1201 tgacaggcgt tggaatactg agcgaacggt tttacttcgc ttgccacagg aggatatgtg
     1261 tcagacattc ggtttacctt catcggtgaa atatgaatca gatggaggcc caggcatcgc
     1321 gcggatcatg gcgtttttga tggggtccag cgaggcgctg aaagatcgct atgattttat
     1381 gaagtttcaa gtgttccagt ggttgattgg cgcaacggat ggccatgcaa aaaacttctc
     1441 cgtatttatt caggctggcg gcagttatcg actcacgcca ttttacgaca tcatttcagc
     1501 atttccggtc cttggcggta cgggaataac atcagcgatc tcaaactggc aatggggctt
     1561 aacgcatcca aaggcaaaaa aacggcaatc gataaaattt atccgcgaca ttttttggcg
     1621 acagcaaagg tgctgagatt cccggaagtg cagatgcatg aaatcctaag tgactttgcc
     1681 agaatgattc cggcagcact ggataacgtg aagacttcat taccgacaga ttttccggag
     1741 aacgtggtga cggcagttga aaccaatgtg ttgaggttgc atggacggtt aagccgagaa
     1801 tacggtagta agtgagatat ggggtttggt cgttgttaat gagcaatcat gaccggccaa
     1861 cgactggatt ctcccttggt ttatgtttgt gcgcataatg tatattatgt taaatcatgt
     1921 ctgagtgatg cgaagtgctt ttctggtagt cgttattcgt tcagattagg tgtccttgtg
     1981 atttaaagat gttatcgtgg cacgaatata ctgccattcc aggctcgtta tattgctctc
     2041 gggcagtccg tagctggtac tgcaaacgct gatgcgacct tcaaagttca atatctataa
     2101 ttcaatctta aggtggctat acgtcatctg aaattgaaac agggtgtgtg tggcatggct
     2161 acgcacaccc gagcaattca ccaatgtgaa tctcattatt aatgaggagt ggtttgtatt
     2221 tggagcgcta tgaaaaatca cataatatct aaact
//
LOCUS       DAJTYZ010000289         2226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000289 DAJTYZ010000000
VERSION     DAJTYZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.294"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            4..90
                     /locus_tag="OO596_004280"
     tRNA            4..90
                     /locus_tag="OO596_004280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Leu,seq:cag)
     gene            125..211
                     /locus_tag="OO596_004281"
     tRNA            125..211
                     /locus_tag="OO596_004281"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:159..161,aa:Leu,seq:cag)
     gene            complement(250..486)
                     /locus_tag="OO596_004282"
     CDS             complement(250..486)
                     /locus_tag="OO596_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6719938.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            627..1415
                     /gene="fhuF"
                     /locus_tag="OO596_004283"
     CDS             627..1415
                     /gene="fhuF"
                     /locus_tag="OO596_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6719939.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1453..2130)
                     /gene="bglJ"
                     /locus_tag="OO596_004284"
     CDS             complement(1453..2130)
                     /gene="bglJ"
                     /locus_tag="OO596_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6719940.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(2088..>2226)
                     /locus_tag="OO596_004285"
     CDS             complement(2088..>2226)
                     /locus_tag="OO596_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR6719941.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT          574 a          580 c          507 g          565 t
ORIGIN      
        1 tgggcgaagg tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt tcttacggac
       61 gtgggggttc aagtctcccc cctcgcacca aacgaggcga tatcaaaaaa agtaagatga
      121 ctgtgcgaag gtggcggaat tggtagacgc gctagcttca ggtgttagtg tccttacgga
      181 cgtgggggtt caagtccccc ccctcgcacc aattatcttt acttcctttc ttgtttcttc
      241 cttgattttt catccctgat tcaaattcat tatcatcagc atgatggcag caataagcgc
      301 caggcacgat ggtagcaaaa cgtaatgccg taactgtttg tgatacagca tcaccggcgt
      361 caatactaat cccatcagaa tgacaatccg ccactgatcc ggcgataaat ccgcatacat
      421 cagccctgca atcagcaatc ccggcagcaa cactccccag ccactgccca gcgtacgttg
      481 caacatggtt tcatctcttt cattgataat gataaccaat atcatatgat aatttttatc
      541 atttgcaagc cagagaaatc ccttgctatc gggtaaacct atcgctagga ttagcaatca
      601 ttatcattta gattactatc ccgattatgg cctatcgttc cgcaccgctc tatgaagata
      661 tcatctggcg aacgcatctc cagccgcagg atgctgggct tgcacaagcc gtacgtgcga
      721 cgatcgccaa acatcgtgaa catttgctcg agtttatccg cctggatgaa cccgctccgc
      781 ttaacgccat gacgctggcg caatggtcat caccgaatgc gctaagttct ctactcgcgg
      841 tctattccga tcatatctat cgcaaccaac caacgatgat ccgcgagaac aaaccgctga
      901 tctcactatg ggcacaattg tatatcggcc tgatggtgcc accattaatg ctggcgctac
      961 tgacgcagga aaaggcatta gatgtgtcgc cggaacattt ccatgctgag tttcacgaaa
     1021 ccggacgcgt cgcctgtttc tgggtcgatg tgtgtgaaga taaaaacgca acaccacatt
     1081 cgccgcagca gcgaatggaa acgttaatca gccaggcgct ggttccggtt gtgcaggcac
     1141 tagaagcgac cggagagatc aacggcaaac ttatctggag taataccggt tatttgatca
     1201 actggtatct cactgagatg aagcaactgc tcggcgaggc gacggttgaa tcgctgcgcc
     1261 atgccctctt ttttgagaaa acgctcacga acggtgaaga taatccactc tggcgtaccg
     1321 tcgtgctgcg cgatggcctg ctggtgcgcc gaacctgctg ccagcgttat cgcttaccgg
     1381 atgtgcagca atgtggcgat tgtacgctga aatgaaagca ctgccgggga agtaaacccg
     1441 gcatcatgcg gattaatagg gatgcaacac attacttgct tcgcaatgcc gcatacataa
     1501 cagaatatct gcggcctcca acaaccctgc atcagaactg acgcccagtt tcgacatcac
     1561 attaaattta tgtgcacgga tcgttttgat attgcgttta agctgctcgg caatttgtgt
     1621 cattgagtag ccacgcgaca taaagcgcaa tatttctctc tccgtcgggc ttagcgtccg
     1681 gctttggtta atgtaccact gattgttcag tcggtcggtc gcctgacgta caccatttaa
     1741 tgacaaaaag agttcctgat gaaaaatctc cagcgttgac gctttactta atacaccgtc
     1801 cagcggtgat ggcgacaatg aaccaatcag ccgagcttca atatcatcat ccgcaataac
     1861 taaacgccgg gtacgcggaa acttaatcgc cagttcagtc aggcaagata atccctctct
     1921 gcgctcactt ctcatggcag aaaaagaaaa aatgaccgcc gcaaatgaga tccgcgacat
     1981 ggcatcctga aatgactcct gactggtata ggtatggagc ttataagggt tacccgcaaa
     2041 ctttctgaat aaactctcaa taccgatact gctcatgacg catttttcta tgagtgcgac
     2101 atttctcttc ttaattcggc tgtgttccat tctgcggcat ctccataaac agaaggcaag
     2161 ttaagttctc ttgcaccctg cgcgctattt atccatgcat acatttcagc gttgctgtga
     2221 attgcc
//
LOCUS       DAJTYZ010000290         2179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000290 DAJTYZ010000000
VERSION     DAJTYZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.295"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..715)
                     /locus_tag="OO596_004286"
                     /pseudo
     CDS             complement(1..715)
                     /locus_tag="OO596_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(768..1364)
                     /locus_tag="OO596_004287"
     CDS             complement(768..1364)
                     /locus_tag="OO596_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6719942.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1391..1993)
                     /gene="yadL"
                     /locus_tag="OO596_004288"
     CDS             complement(1391..1993)
                     /gene="yadL"
                     /locus_tag="OO596_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCR6719943.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2008..>2179)
                     /locus_tag="OO596_004289"
     CDS             complement(2008..>2179)
                     /locus_tag="OO596_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6719944.1"
                     /translation="DATFLKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGN
                     FRALATFNFIYQ"
BASE COUNT          606 a          474 c          434 g          665 t
ORIGIN      
        1 cttggcctgc gccataggct ttaaatgaat acagataatt tgatgagctg gataatttga
       61 tctgttgagt tactgttgga tcaaagtttg tatctgtata gaattcaatt gtaagggtct
      121 gataaatacc acctacagcc cagtatttac gggtgctatt cgcattattg caactccaat
      181 ataacacgtc ttcgcttgga ttataccacg aaaaatattg ttctgcagag tcaccaatgt
      241 atatcccagg tgaactaacg ttagttacgg taccgtaagc tgaccacatg tttgaaatta
      301 ataatgtgta atacaaacca ggaactgatg tattaaataa tttatgggag ccatacattt
      361 tacctgagtc aaccattgca ttttcaatac gcattgcgct accacttgtg ctatcacttc
      421 tgttgcaata aagaaaacca ccaccagaag cagtatctaa ctgactctac tgtaatttta
      481 cacctgtagg tccaagctgc gtcggtttgt agaaaactaa attatttgta cttgttgcat
      541 atctgaatat gacagtagca tcgccattcg gccccgtttc tctccatttt tgatctgacg
      601 gaccaacgcc gtaactattc ccaacaatat atcctacgtt atcatcagtg ccagcactcg
      661 cggtataaca actcaagcac agaattaaaa aaaaatgtct gaagaatttc ttcattatca
      721 ctccaccatc tactgctgta acagtcgcag ttatttgttt tgatctatta ttcgtagtac
      781 acatcaacta atatatgact cattacttgg ccagatatga catccagcgc gggatcgatt
      841 ttttgcattc tggcataaaa tttccatgaa gaattatgca gggcagaagt cattactgga
      901 tacacggaac gagaatttcc tgctgaacta ataacattga atatattggt cgggtcctga
      961 atggaaaaga gaacgacacc gacattttta gccccggttg cgacttcatt ggcaaatatt
     1021 tgccttgaac ctgcagccat gacaccgcta agggctctaa aatcaatatg taactgatta
     1081 agcacatcgc cagttccctg taatgcacag tctgatactg tgacagtgaa ctcttttcca
     1141 cctggttgat agctttcagg agtaacatta tctgaaaaat atcccacccc aacgacgcca
     1201 agatcaatat taccctggtt actgattccg cttttgcaag tgctgttttt tacattggca
     1261 acaaggtcaa tatcctgccc agcgatggct ggataggtaa tgggaaactg tagtgacaga
     1321 aacagaatta ttctcttcat cagcttacgt tgagtgggat gcatatatag ttcctggatc
     1381 tgtcagtctg ttagttatag gttactgtaa atgtggcagg tgcagaaaat ttacccgcag
     1441 tgacattgct tacggtttta tttttttcga cgaccagtcg agcactcatc ggataataaa
     1501 caaccgcgtt ggctgcattg gagatagtga cctcttgaga tgctggtgtc acacaactaa
     1561 attgtgttgc gctccctgtt gccggagttt cggtgctcca gacttcaacg gcgacggcac
     1621 ttgctttatc tgcggctgtg gatccatttg cgagccccgc accgtcatta gcagtacctt
     1681 cgcatgtggc tcgcttggtt aattttattt gtgctttttt attggggata cccgcacagt
     1741 ctttgaattt gagtttgaat gttttaactt tggtcttggc attgatttca gaaatatata
     1801 catcgccaaa gtcaacaaca gaaatgtcct gatcagagtc atttactaat gtagcggtac
     1861 aagtacctat ttctacggta gtaatcacgg tcagcccaat gctgtctgtt gccgcataag
     1921 agggtacgct gaacaatgag gcagccaaaa cgacactgct ggataatcca taacgtaaat
     1981 ttttaaatgt catcttaatt atccttctta ttgatagatg aaattaaacg ttgccagtgc
     2041 acgaaaattc cccggtacgc cttgccctgc atctgtttca cgtagcgcaa cggtcatttc
     2101 aagaccctta tcgggctgca tctcgtctgt gctccagcgt atcttttccg cactgttagg
     2161 tttaaggaaa gtggcatca
//
LOCUS       DAJTYZ010000291         2138 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000291 DAJTYZ010000000
VERSION     DAJTYZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.296"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1138
                     /locus_tag="OO596_004290"
     CDS             <1..1138
                     /locus_tag="OO596_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6719945.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="OO596_004291"
     CDS             1150..1599
                     /locus_tag="OO596_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719946.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
     gene            1861..>2138
                     /locus_tag="OO596_004292"
     CDS             1861..>2138
                     /locus_tag="OO596_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6719947.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFIN"
BASE COUNT          650 a          348 c          570 g          570 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttactcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggcgacat gacccgcagg gaaagatcat cagaagttca
     1741 ctttttgtac taaataattc gcattttatg tttaaaaatt gagatattcc ttattaccta
     1801 aagctgtttt ttattgctta tacatgatca aatacttctt acataattaa ggagaacaaa
     1861 atggaactta aaaaattgat ggaacatatt tctattatcc ccgattacag acaagcctgg
     1921 aaagtggaac ataaattatc ggatattcta ctgttgacta tttgtgccgt tatttctggt
     1981 gcagagggtt gggaagatat agaggatttt ggggaaacac atctcgattt tttgaagcaa
     2041 tatggtgatt ttgaaaatgg tattcctgtt cacgatacca ttgccagagt tgtatcctgt
     2101 atcagtcctg caaaatttca cgagtgcttt attaactg
//
LOCUS       DAJTYZ010000292         2101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000292 DAJTYZ010000000
VERSION     DAJTYZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.297"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(60..1025)
                     /locus_tag="OO596_004293"
     CDS             complement(60..1025)
                     /locus_tag="OO596_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR6719948.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1891..>2101)
                     /locus_tag="OO596_004294"
     CDS             complement(1891..>2101)
                     /locus_tag="OO596_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719949.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          582 a          551 c          439 g          529 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatat accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gctgaggata atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca
     1861 cctcagcctg ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt
     1921 gataccgttt aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat
     1981 atataaaatt aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact
     2041 taatccatca ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct
     2101 t
//
LOCUS       DAJTYZ010000293         2084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000293 DAJTYZ010000000
VERSION     DAJTYZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.298"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1484
                     /gene="tssI"
                     /locus_tag="OO596_004295"
     CDS             <1..1484
                     /gene="tssI"
                     /locus_tag="OO596_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6719950.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRV
                     GLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGT
                     KIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            1552..2084
                     /locus_tag="OO596_004296"
                     /pseudo
     CDS             1552..2084
                     /locus_tag="OO596_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          502 a          535 c          657 g          390 t
ORIGIN      
        1 tctgcatcag ccagtttcgc tacagcgcac aaatccgtcc ttcttccgta gtgaccaaag
       61 actacacctt taaacgtccc ggctggccag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag ggtgcccacg
      181 ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcgagcga tcttcacggc gaacagccgc
      361 aggcggtacc gggacgcagg ggttcaggca ccacgctgga gaaccatttt gcggtaatcc
      421 cggcagacag aacatggcgg ccacagccgt tgatgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggct ttggcaacct ggcgataccg cgtgtgggtc
      661 aggaggtgat tgtggacttc ctcaacggcg acccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggca gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgctg aaaccattgg caacaatcag atgattgcgg
      961 ttaccaacaa tcagatagag acggtgggcg ttaaccagat agagacggtg ggcagtaacc
     1021 agataattaa agtgggttct gttcaggtgg aaacgattgg acttgttcgt gcgctgacgg
     1081 tgggcgtggc gtatcagacg acggtaggtg gcattatgaa cacctcggtg gcactgatgc
     1141 agtcctcgca gatcggtttg cataaatcgt tgagggtcgg gctgggttat gacgtcaaag
     1201 tcggaaataa cgttaccttc accgttggta aaacgaaaaa ggatgatacc gggcagaccg
     1261 cgatttactc cgccggtgag catctggagc tctgctgtgg taaggcaagg ctggtgctga
     1321 cgaaggacgg acaaattttt ctcaacggca caaaaattca tttgcagggt aaggagcagg
     1381 ttaatggtga ctcactgttg attaactgga actgtgcagc ctcgaaatcc ccaccgaaga
     1441 cccctgatga aaagcaggat acgccggata tgagagagta ctgatcccgt acctgttcgg
     1501 tattcagtaa caaaccagca ttaaaaataa acagaagaag gaatttgaat catgagcgga
     1561 aaaccagcgg cgcgtcaggg agatatgact cagtatggcg gtcccattgt ccagggttcg
     1621 gcaggtgtaa gaattggcgc gcccaccggc gtggcgtgct cggtgtgtcc gggcgggatg
     1681 acttcgggca acccggtaaa tccgctgctg ggggcgaagg tgctgcccgg cgagacggac
     1741 cttgcgctgc ccggcccgct gccgttcatt ctctcccgca cctacagcag ctaccggacg
     1801 aagacgcctg caccggtggg cgttttcggc cccggctgga aagcgccttc tgatatccgc
     1861 ttacagctac gtgatgacgg actgatactc aacgacaacg gcgggcggag cattcacttt
     1921 gagccgctgc tgccggggga ggcggtgtac agccgcagtg agtcaatgtg gctggtgcgc
     1981 ggtggtaagg cagcacagcc ggacggccat acgctggcgc ggctgtgggg ggcgctgcgc
     2041 cggatatccg gttaagcccg catctttacc tggcgaccaa cagc
//
LOCUS       DAJTYZ010000294         2040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000294 DAJTYZ010000000
VERSION     DAJTYZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.299"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>2040
                     /locus_tag="OO596_004297"
     CDS             <1..>2040
                     /locus_tag="OO596_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719951.1"
                     /translation="AILGYQANSNGKVIVEKGGEWLIKNNDSSIEFQIGNQGAGEATI
                     REGGLITAENTIIGGNATGIGTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNK
                     EYSLVGVQDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGII
                     TAGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENG
                     VGNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNL
                     MDATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNL
                     KTSSTNAQLLQVGVLGTGELNITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFN
                     MYVGTSGTGTLTLTNSGTLNVEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNAT
                     KVEFGSGEGVFVFNHTNNSDAGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTL
                     VNDGLLTIASHTADGVTGMGSSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRV
                     QLSSYDKMFGFTHATGTEFAGVAQLKDRTFTLERDNTAALTHAMLQSDSENTTSVNVG
                     EQSIGGLAMNGGTLIFDTDIPAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTG"
BASE COUNT          565 a          409 c          570 g          496 t
ORIGIN      
        1 cgccatttta ggatatcaag cgaacagtaa tggtaaggtt atcgttgaaa agggtggcga
       61 gtggctaata aaaaataatg attcctcaat tgaatttcaa attggtaatc aaggagctgg
      121 ggaggcgact attcgcgagg gtgggctgat tacggctgaa aatacgatta tcggtggcaa
      181 tgccaccggt atcggaaccc tgaatgtgca ggatcaagac tctgtcatca cggtacgcag
      241 actctataat gggtatttcg gtaatggcac agtcaatatt tccaataatg gtctgattaa
      301 taacaaagaa tattcattgg tgggcgttca ggacggttcc cacggtgtcg tcaacgtgac
      361 cgataaaggg cattggagtt tcctcggaac aggcgaagct ttccgctata tctatatcgg
      421 tgatgctggt gacggtgaac ttaatgtctc gcgtgaaggt aaagtagatt cgggaattat
      481 cactgcgggg atgaaagaaa caggcacagg caacattact gttaaggata agaactccgt
      541 tatcactaat ctcggaacta atcttggtta tgacggccac ggcgaaatga atatcagtaa
      601 tgaggggctt gttgtcagca acggaggaag ctcactcggt tatggagaaa acggcgtcgg
      661 gaatgtcagc atcaccacgg gggggatgtg ggaggtcaat aagaatgtct ataccaccat
      721 tggtgttgcg ggcgtcggaa acctcaatat tagcgatggc ggtaagtttg tatcgcaaaa
      781 tattactttt ttgggcgata aagcaagcgg tatcggcaca ctgaacctga tggatgcgac
      841 atcatcgttc gatactgtgg gtatcaatgt cggtaatttt ggtagcggta tcgtaaatgt
      901 cagtaatggt gccaccctta attcaacggg ctatggattt atcggaggaa atgcctccgg
      961 taaggggata gttaatattt caacggacag tctctggaat ttaaagacat catctactaa
     1021 cgcacaattg ctacaggtcg gtgtattagg cacgggtgaa ctgaatatta ccaccggagg
     1081 tatagttaaa gcgcgtgata cacagatagc tctcaatgac aaaagtaagg gcgacgtgag
     1141 ggtggatggg cagaactctc ttcttgaaac attcaatatg tacgtaggga catctggtac
     1201 gggtacgtta accctgacga atagcggcac gctgaatgtc gaaggtggag aagtttactt
     1261 aggtgttttt gaacctgctg taggaacgct aaacattggt gcagctcacg gtgaggcggc
     1321 ggcagatgcc gggtatataa ccaacgcgac gaaagtagag tttggttctg gcgaaggtgt
     1381 ttttgtcttt aatcatacta ataacagtga tgccggctac caggtcgata tgctgattac
     1441 aggtgacgat aaagacggaa aagtgatcca tgatgcaggc catacggtgt tcaatgcagg
     1501 gaatacttat agcggtaaaa cgctggtcaa tgacggcctc ctgaccattg cgtctcatac
     1561 ggcagatggg gtaacgggca tgggttcgag tgaagtcacc attgcaagcc ccggtacgct
     1621 cgacattctc gcatcaacga acagtgcagg agattacacg ctgaccaatg cgctcaaagg
     1681 cgatggcttg atgcgagtgc agctgtcatc ctacgacaag atgtttggct ttacccatgc
     1741 aacagggact gaattcgccg gtgttgccca actgaaagac agaaccttca ctctggaacg
     1801 cgacaacacc gctgcgctta ctcacgcgat gttgcagtct gacagtgaaa ataccacatc
     1861 ggtaaacgtg ggagagcaat ccattggtgg actggccatg aatggcggta cgctcatttt
     1921 cgatacggat attcctgctg cgacgcttgc agagggatat atcagcgtcg atacgctggt
     1981 tgtcggcgcg ggtgactaca cctggaaagg ccgtaactat caggtaaacg ggacgggcga
//
LOCUS       DAJTYZ010000295         2036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000295 DAJTYZ010000000
VERSION     DAJTYZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.300"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>2036)
                     /locus_tag="OO596_004298"
     CDS             complement(<1..>2036)
                     /locus_tag="OO596_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6719952.1"
                     /translation="YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTE
                     QVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDE
                     AGRLKAQTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGLRS
                     SRKYDRQGRLAEETSRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     PVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQ
                     WQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAY
                     SEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAY
                     ELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVH
                     TTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAE
                     KTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT          302 a          715 c          561 g          458 t
ORIGIN      
        1 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
       61 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
      121 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
      181 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
      241 gtgagggtgc tgtccgggtg cagttccggg tccggcagcc gatttcctgc cgggtctgtt
      301 gcatacggga tatcgatatc caggttcgct gccgtggtgt ggacgcccgt cagcctcccc
      361 gtgtcgctgt aacggtactc ccggctctcc tgcgggccac tgatgcggat tagctgtccg
      421 ttgtcgttcc agtcgtagtc acggtcaagc tgcggaaggt tcaggtgcct gctccggagc
      481 tggccgctgg tattccaggc ggtggcaagt tcatatgcct ccccgccgaa gctgcgggcc
      541 gtctcacggt gcagccggtc ccgcgtgtac tcgaccagcg gtgttccgcc cagcttcatc
      601 cccgcaagat aaccgctgcc atacgtcagc cactccaccg gcggcaggcc gtccggctcc
      661 tgacgggtcg ccagcccctg ttcgctgtac gcgtgccccg tctcatgctc ccacagcatc
      721 tcccccgtct ccggggtctc caccgtctgc cgttcgcccg tcagacggcc tttatcgtca
      781 tagccgtagt ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag
      841 ccgtggtcgt catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata
      901 cggtccgacg catcgtaatg ccagagggtg ataagcccct catcttcact ctgcgtgagt
      961 tttccggtca ggtcatactg gtaacgttgc gtccggccgt caaagccgcg ctgctccgtc
     1021 agcctgtcga ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcaca
     1081 gtgatgcgcc cggcggcgtc ataccccatg cttcgcgtca gaccgccctg cgtggtgctg
     1141 acggcctttc cccacgcatc atactgtatc tcactgcggc tgccgtccgg ggcaacgata
     1201 gcggtgaggt ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccctc
     1261 tgactgacca gttgaccgcg cgggttataa ctgctgtaag tgctgatgcc ttcttcccgg
     1321 tgaacggcga tttgttgacc gtaccggtca tactcatacc gcgtcgtgta ccccgagcag
     1381 tccgtaaacg cctgaagctg accgtaccgg tttcgcgtta ttctccgcct caccccggtt
     1441 ccgtcttcca ccgcacaggg caggccactg cgggaagaat cataaaacca gcgcgtaatg
     1501 ttaccgttgc gcgaggtctc ttccgccagc ctgccctgac ggtcatattt ccggctgctg
     1561 cgtaaaccat ccggatatgt cactgacgtc agttgcaact ggttgttgta accataccgt
     1621 accgtcctgc cgtccggcag taccactgaa gtcagctttc cagacgccat atgcagccgg
     1681 tactccgtcc gccgccccgc cgcatccgtc tgcgccttca gcctccccgc ctcatcatac
     1741 tcgctgcggg tgatgctccc gtcggcatgc tccttcttca ccacccgctt caggccaccc
     1801 tcgccttccg tgtacagcac ctcccgccgg ttcaggctgt ccgtgatgat tacccggctc
     1861 tctccgtact caaagcggta gtccagcccc tccgggttga cctgctccgt cacccgaccg
     1921 gtatcatcat accggtagcg gctctccggc ctccccgcat aatggtgcgc caccatccgc
     1981 ccggcgtgct ccgcatcata agtaaacccg cgaacctgcg tcccgctgcg gtcata
//
LOCUS       DAJTYZ010000296         1985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000296 DAJTYZ010000000
VERSION     DAJTYZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.301"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            270..539
                     /locus_tag="OO596_004299"
     CDS             270..539
                     /locus_tag="OO596_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719953.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            536..762
                     /locus_tag="OO596_004300"
                     /pseudo
     CDS             536..762
                     /locus_tag="OO596_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            791..1381
                     /gene="ospG"
                     /locus_tag="OO596_004301"
     CDS             791..1381
                     /gene="ospG"
                     /locus_tag="OO596_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6719954.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1720..1985
                     /locus_tag="OO596_004302"
                     /pseudo
     CDS             1720..1985
                     /locus_tag="OO596_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          562 a          434 c          468 g          521 t
ORIGIN      
        1 tgactgacga ggtactggcc ctgcgattgt ctgaaaacgg ctcacaactg caccattcat
       61 aatcacatcg cataaaactg tcagcgccag tgataggaca ggtgaaacct gtcaatacgt
      121 accggcttac gccgggcttc ggcggtttta ctcctctgcc atatgcaaca acggagtgcc
      181 gccttacatg ccgctggcgc ggcatatcct ggtgacgata tctgaacagt tatatactgt
      241 gtatatacgt ggtaatgacg aggtggcaaa tggcacaggt taatatgagt gtaagaatcg
      301 acgcagaact gaaggatgct tttatggccg ctgcaaaaag catggaccgt aatggctctc
      361 agttaatccg ggattttatg cgccagaccg ttgaacggca gcacaattct tggttccgtg
      421 accaggttgc ggcaggccgt cagcaactcg aatgcggtga tgtgcttccc catgacatgg
      481 tcgaatcctc tgctgctgca tggcgggatg aaatgagcag gaagattgct gataaatgat
      541 ggagatgttc tggaccatgc tggccatcca ggacaggaag cgcattcgta gtacattgcg
      601 gagcagaacc tgatggccgc cattgaaccg gacgaacgga ttggttattc agccagcagc
      661 ctggcagggc agccgtataa aggccgcaac ggtcgtgtgg aaggaacatc tggaccacac
      721 aaggtagcgt gtaacgttat tttgtgtgaa aatctcttgt gatttttaaa tatcgtaaca
      781 aaggtatgac atgaaaataa catctaccat tattcaaaca ccttttccat ttgagaataa
      841 taattctcat gctggcatag taacggagcc cattctcggt aagttaatag gtcaggggtc
      901 gacagcagaa atctttgaag atgtgaatga ttcatctgct ttgtataaaa agtatgatct
      961 tattggcaac cagtacaatg agattctgga aatggcttgg caagaatctg agctttttaa
     1021 tgctttttat ggcgatgaag catccgttgt tatacagtat ggcggagatg tgtacctccg
     1081 aatgctgcgc gtgcctggga ctccccttag tgacattgat acagctgata tccctgataa
     1141 tatagagagc ctttatctac agttgatatg taaattgaat gagttgagta taatccatta
     1201 cgatcttaat acaggtaata tgctgtatga taaagaaagt gaaagtttat tcccaataga
     1261 ttttcgcaat atttatgctg aatattacgc tgcaaccaaa aaagataaag agattatcga
     1321 ccgacgatta caaatgcgta caaatgattt ttattcgtta ttaaacagga aatatttata
     1381 gccgtatttg ttgatgctat aagccagagg ctcttctatg agaatatggg gaccattccc
     1441 tcgcgatatt aacgtaatat cttaattgta tgcaccgcca gaatgcgttc agtgcgatca
     1501 cggcagcaga caggtaaaaa tagcaacaaa ccacccgaaa aaccgccgcg atcgcgtatg
     1561 ataaatttta accacatgca tacctgtgca gcaaggcgaa tcacgcacgg atttccagcc
     1621 agagacacct gaaacgtaac cggctcattt aaaccgtctg gtttgttacc tccggttcca
     1681 caaaaataat gtccatcatt ttgaagtgga cactatcgta tggaataccg gacctgaatc
     1741 actgaagctt tacgccttca tttcgaagaa catttaccac gggttgtggc cggacgtcgc
     1801 ctgggcgtac caaaatcaac tgtctgtggt atgttcgtgc gcttccgcaa cgctggcctt
     1861 tcatggcctc tgcccgcagg tatgtcggag caggaacttg atgcccttct ttacggaagt
     1921 gcctccacag tacctgtcgt acttactgaa agcaccgtta tgcccaaact cccggtggta
     1981 aaaaa
//
LOCUS       DAJTYZ010000297         1927 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000297 DAJTYZ010000000
VERSION     DAJTYZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.302"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            257..592
                     /gene="iraM"
                     /locus_tag="OO596_004303"
     CDS             257..592
                     /gene="iraM"
                     /locus_tag="OO596_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6719955.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(873..1004)
                     /locus_tag="OO596_004304"
     CDS             complement(873..1004)
                     /locus_tag="OO596_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR6719956.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1504..1580)
                     /locus_tag="OO596_004305"
     tRNA            complement(1504..1580)
                     /locus_tag="OO596_004305"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1544..1546),aa:Arg,seq:tct)
     gene            complement(1681..1756)
                     /locus_tag="OO596_004306"
     tRNA            complement(1681..1756)
                     /locus_tag="OO596_004306"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1721..1723),aa:Ile,seq:cat)
BASE COUNT          637 a          475 c          334 g          481 t
ORIGIN      
        1 ctttccacca tctgaaaaat aattctttaa aatacctgac atgtaataca gaaaaaacac
       61 aaaaccatac cttaaataaa aacctgatta tcaagcagat atgcatggat aaactacaag
      121 acgagatata aaccaccctg catttaaata aacaataaac aacatcagaa aaataattct
      181 gctctatggt ttacattcaa aaatatcatt tatacttttc agaacatcac cagcaaggca
      241 taaacaagga aactaaatga agtggattgt gattgataca gttatccagc catcatgcgg
      301 aatatctttt tcagtcatat ggagtaaaat aaaattaata atctggtatc aatcggatgc
      361 tttcttacct cctgaaagta tatttacact gactcacaca ggcatcatgc tcaataacaa
      421 agtgctacct gtaaccattt acaacgtagt accattcaat aaaacattct ggaattcaat
      481 caaaaatagc caggaatgcc ctacaaatac agataacgta ttgaatgaat gctttaataa
      541 ccgttgcact ctgcaaatat gtccttatgg actaaaacaa caaagtccat aaggagttta
      601 ctcacatctg acaaaatcaa tataaacagc ccctccggag aggggctgga gagtggcgct
      661 atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc agtaccagca cctgaatccg
      721 cgattatccc atatacctac tcgctgattg cccctccgca cagggggatt caccatgcca
      781 gtttctttta acaaactccc cgcaaaccag acaacagtca accgcctgaa ttgtgaagta
      841 tttaaaaatt tctcccgcta actgataccc ggctaacagt ctggcgtttt ctttttcagc
      901 aacgggaaag caacaaccac cacacccgcc accagcacac cgtcagccag cactgacatt
      961 atccggctgc tgcaatgcca ttcacaaaaa cagtaagcaa tcacttttta ccgtaacagg
     1021 tgataatcca gatatgtatc taccccagat gagtaatccg aagttcatcc ataccacagg
     1081 tcctggctat tctgttgtac tcctgaacaa gagcaaataa ttctgaatta gcaaccatga
     1141 actcatcgca aaccctctgt atagcatcac tattcagaat aataacgtct cttcccgaaa
     1201 gacgatcagg agtacagaac aaaactgtca aacggctgaa ggcctttgct cgtgctgcat
     1261 tgactatatc aatacgctgc ctaaggatga aacaccccga cgcctcatca atattcactc
     1321 tacccacacc atatgaatga taaatattta atgctgaaaa aaccattaga ccgtataaca
     1381 aacactcaat caatacttaa cagaactttt atttttgaca aacataaaat attttcaaca
     1441 atatcctgag ccaggtatat ttcagtataa ggctctgccg gaaggaatct ggaagaatga
     1501 atatggcgcg ctgtactgga ttcgaaccag tgaccgattg cttagaaggc aattgctctg
     1561 tccggctgag ctaacaacgc tgaataccga taatggaccg ccatcgggga cccgcccccg
     1621 caccaacaac cctgttatcg tgtcgtctgc tcttcctgat aagctaatgg cggtttgtga
     1681 tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct cgctctcacc
     1741 actgagctaa agggccggga gcagaataat aacggtccgt aattaattcc gcaataaaaa
     1801 acccgctcgg cggcgggttg tagaaactct tctaacgtca ggcataaaac gcccatagtt
     1861 atgacgaatt taccacagat tccggaaaaa tcaacctagt tacctttttt aactgccgct
     1921 cagccca
//
LOCUS       DAJTYZ010000298         1887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000298 DAJTYZ010000000
VERSION     DAJTYZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.303"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..389
                     /locus_tag="OO596_004307"
     CDS             <1..389
                     /locus_tag="OO596_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719957.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="OO596_004308"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="OO596_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6719958.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="OO596_004309"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="OO596_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6719959.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="OO596_004310"
     tRNA            complement(1806..1881)
                     /locus_tag="OO596_004310"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
BASE COUNT          503 a          446 c          457 g          481 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattag
//
LOCUS       DAJTYZ010000299         1884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000299 DAJTYZ010000000
VERSION     DAJTYZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.304"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            76..345
                     /locus_tag="OO596_004311"
                     /pseudo
     CDS             76..345
                     /locus_tag="OO596_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            460..630
                     /locus_tag="OO596_004312"
     CDS             460..630
                     /locus_tag="OO596_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719960.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(757..1314)
                     /locus_tag="OO596_004313"
     CDS             complement(757..1314)
                     /locus_tag="OO596_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6719961.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1311..1586)
                     /locus_tag="OO596_004314"
     CDS             complement(1311..1586)
                     /locus_tag="OO596_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6719962.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1771..1884
                     /locus_tag="OO596_004315"
                     /pseudo
     CDS             1771..1884
                     /locus_tag="OO596_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          528 a          387 c          457 g          512 t
ORIGIN      
        1 ttttcattga tttcaccatg ttatagtttt atttgtgaat taaatcaatt atggcgatga
       61 attacaaggg gttaaatgct gccgcagcat agcgatattg aaatagccta gtatgcttcg
      121 atacagcagg agccgaatgg ctggaagacc gtcaccacac agttctacat ccaggaattt
      181 agtgagtata ttgcgccact gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa
      241 agatcgttgc tggaggcatg gaataaatat cgggtattgt tgaatcgtgt tgatacatca
      301 actgcacctg atattgagtg gcctgcaaat cctgtcaggg agtaatcatt gggattatgc
      361 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
      421 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      481 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      541 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      601 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      661 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      721 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      781 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      841 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      901 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      961 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
     1021 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
     1081 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
     1141 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
     1201 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
     1261 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
     1321 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1381 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1441 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1501 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1561 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1621 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1681 caggcagtta aaaacacgga aaacggtata aaaaagcgcc tcaaaatcct gttttttctt
     1741 tcagatacaa tgtcttattg cattttttaa aacatgtcag tgtggtacat ggatatcgat
     1801 accatcgcca attataaact gggcggtgca cagcaacatg atagcgtacg cctcgatccg
     1861 tgggtgttta tgttctcagc agga
//
LOCUS       DAJTYZ010000300         1876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000300 DAJTYZ010000000
VERSION     DAJTYZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.305"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..74
                     /locus_tag="OO596_004316"
     CDS             <1..74
                     /locus_tag="OO596_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6719963.1"
                     /translation="RLKGKLTPALLQILIREIKGSSH"
     gene            74..421
                     /gene="tnpB"
                     /locus_tag="OO596_004317"
     CDS             74..421
                     /gene="tnpB"
                     /locus_tag="OO596_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6719964.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            441..1595
                     /locus_tag="OO596_004318"
                     /pseudo
     CDS             441..1595
                     /locus_tag="OO596_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1599..1758
                     /locus_tag="OO596_004319"
                     /pseudo
     CDS             1599..1758
                     /locus_tag="OO596_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          455 a          493 c          523 g          405 t
ORIGIN      
        1 tcaggcttaa aggtaaactg actccggcgt tattacagat acttatccgc gaaataaaag
       61 ggagcagcca ctgatgatat ctttccctgc aggttcgcgt atctggctgg ttgcaggtat
      121 caccgatatg cgaaatggct ttaacggcct ggcctcaaaa gttcagaacg tcctgaagga
      181 tgacccgttc tccgggcatc tgttcatctt ccgcggacgc cggggtgacc agataaaagt
      241 gttgtgggct gacagtgacg gactgtgcct ctttaccaga cgcctggagc ggggccgctt
      301 cgtctggccg gtcacccgtg acggcaaggt gcaccttact ccggcccagt tatccatgct
      361 tcttgagggc atcgactgga aacacccgaa acgaacggaa cgcgctggca tccgcatata
      421 acccgttgta aagtgaggat atggacacct cacttgctca tgagaatgcc cgcctgcggg
      481 cactgttgca gacgcaacag gacaccatcc gccagatggc gaaatacaac cgcctgctct
      541 cacagcgggt ggcggcttat gcttccgaaa tcaaccggct gaaggcgctg gttgcgaaac
      601 tgcagcgcat gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacggcaga
      661 tacaggaagc acaggagcga atcagcgcac ttcaggaaga aatggcggaa acgctgggtg
      721 agcaatatga cccggtactg ccatctcccc tgcgccagtc ttcagcccat aaaccgttac
      781 ctgcctcact tccccgtgaa acccgggtca tccgtccgga agaggaatgc tgcccggcct
      841 gtggtggcga actcagttct ctgggatgtg atgtgttaga gcagctggag cttatcagca
      901 gcgcctttaa ggttatcgaa acacaacgtc cgaaactggc ctgttgccgg tgtgaccata
      961 tcgtgcaggc accggtgcct tcaaaaccca ttgcacgcag ttatgccgga gcggggcttc
     1021 tggcccatgt tgtcgctgga aaatatgcag actatctgcc gttataccgc cagtcagaaa
     1081 tataccgtcg tcagggcgtg gagctgagcc gtgccacact ggggcgctgg acaggtgctg
     1141 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
     1201 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
     1261 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa atgcctccgg
     1321 cggtctggtt cgcgtactca ccggaccgga aaggtatcca tccacagaat catctggccg
     1381 gttacagcgg agtgcttcag gccgatgctt acggtagtta ccgggcgttg tacgaatccg
     1441 gcagaataac ggaagcccag cagcgtatcg gtgaactgta tgccatcgag gcagaagtcc
     1501 ggggatgttc agcagaacag cgtctggcgg caagaaaagc cagagccgcg ccactgatgc
     1561 agtcactgta tgactggata cagcaacaga tgaaaattca ttcgctgaaa atggaatgtc
     1621 tccacggaga acactattat ccatcgggaa atagtgcggg caacagtgtt tagttatatc
     1681 gagtgcgatt acaatcgctg gcgacggcac cgttggtgtg gtggtctcag tccggaacaa
     1741 tttgaaaacc agagcctcac ttacgactgt gtccatatta tgtgggtagg atcaataaag
     1801 acgttttata tgtgtctgct tgcagccaga aaatttatac cctgcaggta tcataaacat
     1861 gcttgacaac ttaatt
//
LOCUS       DAJTYZ010000301         1867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000301 DAJTYZ010000000
VERSION     DAJTYZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.306"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..926)
                     /gene="ospC4"
                     /locus_tag="OO596_004320"
     CDS             complement(<1..926)
                     /gene="ospC4"
                     /locus_tag="OO596_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6719965.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNK"
     gene            1342..>1867
                     /locus_tag="OO596_004321"
     CDS             1342..>1867
                     /locus_tag="OO596_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6719966.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     FKVRLAKQALQPGAVVARIAREHDINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWR"
BASE COUNT          546 a          367 c          351 g          603 t
ORIGIN      
        1 tttttgttat aacaaaactc tctgaatcct tgatctttac tctttcttat aaaatcaata
       61 agatgcaacc caattccaag cctcatatct ttagtgttaa atattggaac atcagttttc
      121 ccctgattac catgcacata cctgctaact tcatccacaa cctctttaaa gttatctaag
      181 aaaaatgatt ggtgctcatg gtaaaaaaca ggtggaatag cattatcaaa gtgatcggtt
      241 aatgtcatat atccatatgg agagtcatga tcaatgatat acgcattggc gccaaaatct
      301 acagtatgat gtgttttatt tagtgggagt ttttcttgat gattggaaat ttcaacccca
      361 aaaaaaacaa aatcgtggtt agagatttct ttaatatcct cagagtatgt attcttatgt
      421 gtttgtatat tttcagactg aattttatca aaggatttaa tattaagaat attatttgac
      481 agattagagt tactttgatg tctgaagtta aaattagaat taataataat agaaagtaag
      541 tgacgttcgt ctgaagaaag aggatgaact ttttgtatta tctcagagat atagtgtctg
      601 gacaccttat cgagatgctc aagtgaccga aatgatgacc gtgctccaga tggcgcttca
      661 atagaaagat ttagtgattt gaaactggtc ccttgagaaa acattttgct ataactttgc
      721 gctgctatag acttccttaa aaagtttttc acagtattag cacagtgatc taaatgtttt
      781 tcacttatgc cagaagaaga tgagtttgtt acgcgtaata tatttttttg taatgctttt
      841 gttgctttat tcggattcgt ttttccatca acaagatcaa tattattctg aacattaata
      901 tgatttactg cttcaggtat tttcatcccc cccccaatta tgtgcgtttt tatctgtggt
      961 tatttagata acattatcta actggacact ttcttgtatg tattatagct aaatgaacag
     1021 attttaatat gttgtatggt tttcactctg aaatcccctg aatttaatca cgtttccttt
     1081 actgaaggca attttaaccg tagtatcaat tattaaccct tgggcttgat atccctctcc
     1141 tcttctgggc acgggggatg tcagattact tattacagaa accaaaagaa ttgtaagcgt
     1201 catctcagtg tcgtctattt caatgtctga tgattggcga tgatagagtc ctcttataag
     1261 taagcgcccc atcagcgacg tcttgtgaaa attgtcctgt ctggcaacaa tcgcgcccat
     1321 ctatattgat ggacacgaac gatgaattcc cagacaacaa aagatattcc ctgcttccgt
     1381 tcttatttgc ctgatgccct gcgtttaaga tttgaagata aactgaccat ccgggccatc
     1441 gctcagcgtc tgggtctcag tcattccaca atacatacgc tttttcagcg atttcttgca
     1501 tccggtatcg catggccatt gcccgattca gtttcattcg ctcaacttga cgccatcctt
     1561 tatgccaaca gaaagaagga attaacagag cctcaaatca gagaaggctc atggcgaaaa
     1621 gaacggcgaa ccagctatag ccgtgaattt aaggtccgtc tggctaagca ggcattacag
     1681 cctggtgctg ttgttgcccg gatcgccaga gaacacgata tcaatgataa cctgctgttt
     1741 aaatggaaaa gccagtacga ggacggctta ctgagcgatg atgacataca ggaatgcatg
     1801 cctgtcccgg tggcactgac tgatacgccg gagccgacca gaccagttac aaatcccttc
     1861 tggcgta
//
LOCUS       DAJTYZ010000302         1817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000302 DAJTYZ010000000
VERSION     DAJTYZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.307"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="OO596_004322"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="OO596_004322"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6719967.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1115..1310
                     /locus_tag="OO596_004323"
                     /pseudo
     CDS             1115..1310
                     /locus_tag="OO596_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1450..>1817)
                     /locus_tag="OO596_004324"
     CDS             complement(1450..>1817)
                     /locus_tag="OO596_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6719968.1"
                     /translation="MMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVY
                     SKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFY
                     DNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          369 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattg
//
LOCUS       DAJTYZ010000303         1813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000303 DAJTYZ010000000
VERSION     DAJTYZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.308"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            21..386
                     /locus_tag="OO596_004325"
     CDS             21..386
                     /locus_tag="OO596_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6719969.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            383..1048
                     /locus_tag="OO596_004326"
     CDS             383..1048
                     /locus_tag="OO596_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719970.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1048..1413
                     /locus_tag="OO596_004327"
     CDS             1048..1413
                     /locus_tag="OO596_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6719971.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          476 a          384 c          504 g          449 t
ORIGIN      
        1 tccgtataag gaaagctgat atggcactga cgaaaaaaca acgtgcagaa ctgcgcatga
       61 agttcggtgg tcgctgcgct tattgcggct gcgaacttgg cgaaaagtgg catgcagacc
      121 atgtaaaacc ggtcattcgt tttgatggaa atatgcttca ccaggaacgt gacgatatat
      181 ccaacatggt accagcatgc cacccatgca atctgcacaa gcattgcagt agcctggaag
      241 attatcgacg aattatcagt gatggtcgtc gtgaattcct tgcgtccggg aaaggcaaag
      301 cgctggttcg tatgggattg gttgaaatga aatctgaccc ggttgtgttc tggtttgaaa
      361 aatatcaaga aggggctacg gcatgaccac tattaccaaa gagcgactgc aatggctggc
      421 taacatttct ggccgcgatg atattgacga tatagacggc ggtgaaattc gtgaactggc
      481 gcttatcgct ctggcatcac tggaagcaga gcctgtagcg gaatgcattg ttgaagatgg
      541 gggcatgtgt attgacgggt tcggtgagta tgtgggtcac tcgctgcctg atggaatgca
      601 tgagctttat gctgccccgc gaatgcgaca acctgatgga tggaaagccg tagctgtagc
      661 gtggaaggtg acgtttactc aagttgacca ggaatctaat acgttcactg ctatatattt
      721 tgacaaagcg gaagttgagt gttgggtacg actgcataaa gcatgtgatt ttcgggcaga
      781 tataacaccg ctttacgcag cgccggcagt gccggttgca gtaaacgacg acatggctta
      841 cgcattccat cacgcactgt cagattcatc gcttggctct gatgaaatcg aagaaattaa
      901 aaccggtttg cgtgctgcct ttgccaacgt cactatccaa ccagagccga tagtgccgga
      961 tgaaatcggg ccaaacgata gtaatacgtt tgattatgtt gatggctgga acgcctgccg
     1021 cgctgccatg cttaagggag ataaataatg attaatcgaa ccaaactgga gcacattctc
     1081 gaatatgcca ggcagcagaa atgtattggg caactttgta aaattccacc aggagatatg
     1141 gttgaaatcg tggaaatggc catgcgtaag gctggcaact ctccggtagc tccggctggc
     1201 tggataagct gtagtgatgc agttcctgct gaatattgcg atgtgattct tctcgatgat
     1261 ctcgggaatg tattccccgg ttcctgggat aaggtttttt gccccactcg tggcgggaat
     1321 aagatggctt ttgtggacaa agacggcgtt gaagtagaga gctcaactca ctggatgccg
     1381 ctaccggaac caccgcagga ggtgaatcaa tgacctggcc tgaggcattc accacggtag
     1441 gaattgcgat ggcggtggcg ctggtggtgt attcgatttg ccgttggggt taacaaacaa
     1501 aaaccccgga ttgatggtcc ggggtttttg aaggaaacaa aacagaaaca gcaattgccg
     1561 ttacctgttg ttaccatggc aagtaaacgt atctcaggcg agcgcattgc gccgttctga
     1621 cgcagataaa ctagcctgga tagatggtgc tggcaataaa aaatagcgtt ttcttatcgg
     1681 tgtcggtaag attgctgcgg gtgcttgagg ctgtctgcct cgggtatgtc actgtaaggc
     1741 agacagagaa aagccccagt taacattacg cgtcctgcaa gacgcttaac attaatctga
     1801 ggcccaatct atg
//
LOCUS       DAJTYZ010000304         1787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000304 DAJTYZ010000000
VERSION     DAJTYZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.309"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1087
                     /locus_tag="OO596_004328"
     CDS             join(<1..183,183..1087)
                     /locus_tag="OO596_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6719972.1"
                     /translation="ERIGVAAAARELSLYESQLYAWRSKLQQQMTSSERESELAAENA
                     RLKRQLAEQAEELAILPKGRDILCEAPEMKYVFIEKHQAEFSIKAMCRVLRVARSGWY
                     TWCQRRTGISPRQQFRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAA
                     SLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGW
                     LYLAVVIDLWSRAVIGWSMSPRLTAQLACDALQMALWRRKRPRNVIVHSDRGSQYCSA
                     DYQALLKWHNLRGSMSAKGCCYDNACVESFFHSLKVECLHGEHFISREIMRATVFNYI
                     ECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            1427..1787
                     /locus_tag="OO596_004329"
                     /pseudo
     CDS             1427..1787
                     /locus_tag="OO596_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          437 a          444 c          478 g          428 t
ORIGIN      
        1 ctgaacgcat cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct
       61 atgcgtggcg cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg
      121 ccgctgaaaa tgcccgcctg aagcggcaac tggcggagca ggcagaggaa ctggctatcc
      181 tccaaaaggc cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa
      241 acatcaggct gagttcagca tcaaggcaat gtgccgcgtg ctccgggtgg cccgcagcgg
      301 ctggtatacg tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca
      361 ctgcgacagc gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc
      421 acgcctgacg gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc
      481 aagcctgcgc cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta
      541 ccgcgcacat ggcctgcctg tgtcagaaaa tctgctggag caggattttt acgccagtgg
      601 tccgaaccag aagtgggcgg gagacatcac gtacttacgt acagatgaag gctggctgta
      661 tctggctgtg gtcattgacc tgtggtcacg tgccgttatt ggctggtcca tgtcaccacg
      721 cctgacggcg caactggcct gcgatgccct gcagatggcg ctgtggcggc gtaagagacc
      781 ccggaacgtt atcgttcact cggaccgagg aagtcagtac tgttcagcag attatcaggc
      841 gctgctgaag tggcataatc tgcgtggcag tatgagcgca aagggctgct gctacgataa
      901 tgcctgcgtg gaaagcttct ttcattcgct gaaagtggaa tgtctccacg gagaacactt
      961 tatcagccgg gaaataatgc gggcaacagt gtttaattat atcgaatgtg attacaatcg
     1021 ctggcgacgg cacagttggt gtggcggcct cagtccggaa caatttgaaa accagaacct
     1081 cgcttaggcc tgtgtccata ttacgtgggt aggatcactt acgccctgtg gttgaaacgt
     1141 gagcatattg ttttcagatg ctgttgtctt gcttcagagt gatctgtcct ccggatttac
     1201 gcaaagttat ccacaggcac tgtgcattta aaaaacttat cttttcttct tgattttaaa
     1261 aactcagatc gtcatatcat tatgataaac ataataaaaa actctaaaca tgagaaggtt
     1321 tttaacgtaa ggtgagagga tttttaagtg tatccataga ttaaaaatga caatgcctcc
     1381 aattagttga attaggggtt ataatacccg cttttaaggt tctttcatgg tcacagttaa
     1441 ccttcattgc ccccgttgcc agtccgttca ggtttaccgt cacgggcaga acccaaaagg
     1501 acatgatcga ttccgttgcc gtgactgtca ccgggtgttt cagctcactt actgttacga
     1561 ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg gctttcaacg gtgccggagt
     1621 tcgcgatact gcccgtacgc tgaaaatcgg catcaatacc gtcatacgga cattaaaaag
     1681 ctcccgcccc ggcggataac atctgcgcca gtcgctcacg ctgatgttgc ccttatctgt
     1741 gaacttgatg ggcagtggag cttcgttggc agcaaggcgc aacaaca
//
LOCUS       DAJTYZ010000305         1769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000305 DAJTYZ010000000
VERSION     DAJTYZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.310"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(423..680)
                     /locus_tag="OO596_004330"
     CDS             complement(423..680)
                     /locus_tag="OO596_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719973.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="OO596_004331"
     CDS             complement(677..1174)
                     /locus_tag="OO596_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6719974.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..>1769)
                     /locus_tag="OO596_004332"
     CDS             complement(1376..>1769)
                     /locus_tag="OO596_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6719975.1"
                     /translation="VNHYRDNAIAYKEQRDNKASELEKANATIADMRKRQRDVAELDA
                     RYIKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGMGDGESPGLTADAELN
                     YYRLRSGIDKITAQVNYLQEYIRTQCLK"
BASE COUNT          393 a          461 c          407 g          508 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaaca
//
LOCUS       DAJTYZ010000306         1768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000306 DAJTYZ010000000
VERSION     DAJTYZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.311"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1517
                     /gene="tssI"
                     /locus_tag="OO596_004333"
     CDS             <1..1517
                     /gene="tssI"
                     /locus_tag="OO596_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6719976.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQ
                     TVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQV
                     GLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKD
                     GSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1593..>1768
                     /locus_tag="OO596_004334"
     CDS             1593..>1768
                     /locus_tag="OO596_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6719977.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVL"
BASE COUNT          452 a          425 c          559 g          332 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc
     1021 tggtggaaac ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg
     1081 tggggatcat tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta
     1141 tgaatacctc ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg
     1201 tgggaatggg ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga
     1261 tgaaggaaaa caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct
     1321 gtggtaaggc aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca
     1381 ttcatctgga aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg
     1441 gtgccacaca acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg
     1501 atatgcggca attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga
     1561 tgaaccatat taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc
     1621 agggcgacat gacccgcaag ggactggata ttgtgcaggg ttcagcgggt gtgctgatag
     1681 gtgcgccgac gggagtggcc tgttcggtgt gtccgggagg gattacctat gctaacccgg
     1741 tgaacccggt gctgggtgcg aaggtgct
//
LOCUS       DAJTYZ010000307         1709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000307 DAJTYZ010000000
VERSION     DAJTYZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.312"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..95
                     /locus_tag="OO596_004335"
                     /pseudo
     CDS             1..95
                     /locus_tag="OO596_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            204..471
                     /locus_tag="OO596_004336"
                     /pseudo
     CDS             204..471
                     /locus_tag="OO596_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            861..>1709
                     /locus_tag="OO596_004337"
     CDS             861..>1709
                     /locus_tag="OO596_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6719978.1"
                     /translation="MIKSTNIQAIGSSIMHQINNIYSLTPFSLPMELTPSCNEFYLKA
                     WSEWEKNGTPDEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          609 a          341 c          269 g          490 t
ORIGIN      
        1 aagcggctgg cacgtaaaac aatctgcttc tcgcgttccg tggaaatcca cgaaaagtca
       61 tcggctcctt cattgaaaaa cacatattct actgattgga agcgccaccc gaaaaagtat
      121 cgttttttta cagtaaactt catttagccg actataatgt aaaaaaataa attttttacc
      181 aaaatataac ataaggaagt aatatgctta cacaaactat attcccttgt cttcccaaaa
      241 aataagaaaa tatcatccct gaagtatcaa atactatatt attatcttct actgttacta
      301 cagatggata tactgttttt aataaaaaaa acagccatat acgaattgca aatacctgct
      361 accaaccgaa cgaaaaggtt gaaattcaca gctacagaaa tacaatggtt gacaaagata
      421 aatgaagccg gaattgataa aaaacaaagc caacggtatt ctgatttctg aagttaattt
      481 cagactcgtt taagctctat tttcaaaaaa atatatcagg gtttgttact aataacttct
      541 gaaaacagaa aaaaatagag cagtaattct atttcagaga ggagtaactc catctatcct
      601 cgatacgtac tgaacgttat cgtgatatat tcatcatgct ttcttcacca ttattcgagt
      661 acagggagag acgcaatgac cttaaatacc tttttatggc tgaattacca ttctcaggaa
      721 taacagcaga ctccttccca tagacggctt agggggaaag caatacccgg agaaagggta
      781 caaagcaaac tgttttaaaa ttaataagct atattctatt tcactagaat tcttatcatt
      841 gataagaaat tttagttata atgattaaat caaccaatat acaggcaatc ggttctagta
      901 ttatgcatca aataaacaat atatactcgt taactccatt ttctttacct atggaactga
      961 ctccatcttg taatgaattt tatttaaaag cctggagtga atgggaaaag aacggtaccc
     1021 cagacgagca acgcaatatc gccttcaata ggctgaaaat atgtttacaa aatcaagagg
     1081 cagaattaaa tttatctgag ttagatttaa aaacattacc agatttaccg cctcagataa
     1141 caacactgga aataagaaaa aacctattaa cacatctccc tgatttacca ccaatgctta
     1201 aggtaataca tgctcaattt aatcaactgg aaagcttacc tgccttaccc gagacgttag
     1261 aagagcttaa tgcgggtgat aacaagataa aagaattacc atttcttcct gaaaatctaa
     1321 ctcatttacg ggttcataat aaccgattgc atattctgcc actattgcca ccggaactaa
     1381 aattactggt agtttctgga aacagattag acagcattcc cccctttcca gataagcttg
     1441 aagggctggc tctggctaat aattttatag aacaactacc ggaattacct tttagtatga
     1501 acagggctgt gctaatgaat aataatctga caacacttcc ggaaagtgtc ctgagattag
     1561 ctcagaatgc cttcgtaaat gttgcaggta atccattgtc tggccatacc atgcgtacac
     1621 tacaacaaat aaccaccgga ccagattatt ctggtcctcg aatatttttc tctatgggaa
     1681 attctgccac aatttccgct ccagaacac
//
LOCUS       DAJTYZ010000308         1698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000308 DAJTYZ010000000
VERSION     DAJTYZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.313"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..73)
                     /locus_tag="OO596_004338"
     CDS             complement(<1..73)
                     /locus_tag="OO596_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6719979.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIY"
     gene            complement(80..400)
                     /locus_tag="OO596_004339"
     CDS             complement(80..400)
                     /locus_tag="OO596_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719980.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIEKANTD
                     SNLR"
     gene            complement(393..590)
                     /locus_tag="OO596_004340"
     CDS             complement(393..590)
                     /locus_tag="OO596_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6719981.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVCHA"
     gene            644..1096
                     /locus_tag="OO596_004341"
     CDS             644..1096
                     /locus_tag="OO596_004341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719982.1"
                     /translation="MRGFVVSIAELHVSALVVFTLHLGEYHFKSFLVFTFVLHCIPLL
                     MGGANMSRSSVNNKSQQLNARFPHEVVSGIEASLQPGETKANFIVTAVRGEIARRQAE
                     GSGENPLVSSLDALANVEQIGIKAAEEIGQLITVAREELQRRKAKESE"
     gene            1154..1330
                     /locus_tag="OO596_004342"
     CDS             1154..1330
                     /locus_tag="OO596_004342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719983.1"
                     /translation="MTNEESTKKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWQRTETD"
     gene            complement(1442..1651)
                     /locus_tag="OO596_004343"
     CDS             complement(1442..1651)
                     /locus_tag="OO596_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR6719984.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGRFPSRLNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAV"
BASE COUNT          474 a          415 c          373 g          436 t
ORIGIN      
        1 gataaatagc gccggggata attttggggt gtgcataacc gctggcagtc aagccagata
       61 aatacgttct catgattatt tatctcagat ttgaatcagt attcgctttc tctatggcat
      121 ttaatgcatc tgtggcattt tcaatggtgc accgtaacga aatatcaaac tgcccaagca
      181 ttgccagtaa caagccaata ttacccatgt caatgcgcat agccttttcg tcatattcct
      241 cattttctga cgcatgccac atcaggctac caattgacgc aacagccatt gatatattgt
      301 cagtagcccc atccgcagcg gaataaacct ttttagcaat atcatgctca cagttaaaat
      361 gcggattaat caggtactgg taattggtca tgtcaggcat ggcacacctc ctgacgaata
      421 cgggcggcga atacagcaac acaacctgac gggcaacggt tacgcgcttc gcgttccgtc
      481 caggcggtta cgtggatgat ttgagattct ccggcactca gggccagaaa acgccacaca
      541 aaggccgttt gtgtgtgtac aaggtgtggt atatgattta cggcaaccat aacggctcct
      601 cgtttacgtt gttggttaga agccctgcga gtggtaacga cacttgcggg gcttcgtcgt
      661 ttcaattgct gaattgcatg tatcagcact tgtggtattc acattacatt taggtgaata
      721 ccatttcaag tcttttttgg tattcacttt tgtattacac tgcatcccgt tattaatggg
      781 aggtgcaaac atgtccagga gttctgttaa caataagtca cagcaactga atgccagatt
      841 tccacatgaa gtagtgagtg gcattgaggc atccctacag ccaggggaaa ctaaagcgaa
      901 ttttatagtt acggctgtac gcggtgagat cgccaggcgc caggcagaag gaagtggaga
      961 aaatcccctt gtgtcttcac tggatgccct agctaatgtc gaacaaatcg gcatcaaggc
     1021 agcggaggaa atcgggcagc ttatcaccgt tgcacgcgaa gaactccagc gccgcaaggc
     1081 caaagaatca gaataatcac tatcagcgcc gtggtgtgag gaactccggc gcattgcttt
     1141 acaggcgcac acaatgacca acgaagaatc aaccaaaaaa acatcacctg cacgaaaaag
     1201 acgacgcaga aagaaagagc atgaatcaga acgattcgcg ccgtgttctt ttgcccttga
     1261 gaagtacctt aaagaatact catcaaagga gagagcttcg cagttatggc aacgcactga
     1321 aacagactga tagcattgcc caccagcctg atagcggcta tcattgacat gcttatgttt
     1381 agtgtttcca aactggcgac cgccccaggt cgcctttgtt ttatgtgtca tatgctcccc
     1441 tttacactgc cttacctgaa ttaatgcgat cccggctttt aacccattcc ataacctcgg
     1501 acagcagcca ccctacagaa cgaccgccca gattaagacg tgacggaaag cgcccttttt
     1561 tctccagttc gtaacgtgta gtgcggcaca ctccagttaa cttacgacat tcatcctcac
     1621 ggattacgcg atcttcattt atttcacgca tacaaaaacc tacataaaaa ttacgtatat
     1681 aaacttttct cttagctg
//
LOCUS       DAJTYZ010000309         1694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000309 DAJTYZ010000000
VERSION     DAJTYZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.314"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..799
                     /locus_tag="OO596_004344"
                     /pseudo
     CDS             1..799
                     /locus_tag="OO596_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="OO596_004345"
                     /pseudo
     CDS             914..1255
                     /locus_tag="OO596_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1213..1694
                     /locus_tag="OO596_004346"
                     /pseudo
     CDS             1213..1694
                     /locus_tag="OO596_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          425 a          409 c          499 g          361 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggccagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtcaggc ccggatgttc atcagttaca gcgaagtcag cataaaaaat
     1261 cccgacaaca gcggccaggc gctcccgctc acgtatgtct gttgccggga gcaggcagag
     1321 gacggtgcct gctggcatct gctgacgtca ggaaaagcgg cgagtgccgc cgatgcacga
     1381 cgtattgtca gccattacga gcgacgctgg ctgacagagg aataccacaa ggcgtggaaa
     1441 agtggtggta catggaatcg ctgcgaatgc agacccggga taaccttgag cgcatggtgg
     1501 ttatccaggc gtttatagcg gtacgggtgc tgggcctgcg gcagggaggt gtcagtgaag
     1561 aaacacaaaa tgacagctgt gagaaaatac tgacgccgac agagtggaaa ctactgtggg
     1621 ttaagctgga aggaaagccg cttccggtgc aggcaccgac cctgaaatgg gcctggggcg
     1681 atggcatgac agca
//
LOCUS       DAJTYZ010000310         1686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000310 DAJTYZ010000000
VERSION     DAJTYZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.315"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(784..1455)
                     /gene="queE"
                     /locus_tag="OO596_004347"
     CDS             complement(784..1455)
                     /gene="queE"
                     /locus_tag="OO596_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6719985.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1594..>1686
                     /locus_tag="OO596_004348"
     CDS             1594..>1686
                     /locus_tag="OO596_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719986.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
BASE COUNT          551 a          344 c          301 g          490 t
ORIGIN      
        1 atcgcactct atcaattttc acttaaagta tgaactggat atctttattc ataagaacta
       61 ttcatcaaca gcgtatagag gcggttatgt aaaaccactc gttagcctca aaacaaagac
      121 aaatttccag atttattact taatcaatga ataacaagct taaaaaacaa actattaacc
      181 taaagaaaaa attaacagac attaattcaa tattaaaaaa ataatagatt aaaatttctt
      241 aacgatttaa gaatcataca aataacactt tgattaaatt taattttttg tatcgataat
      301 tgtgaacccc tctggcatgg agaactattt tgaacatgag gtgttacgtg gatattttgc
      361 ttattacaag tactgctaat ataaaaactt gagaaagaga taacgggtta tatggtggtt
      421 tatccccgct ggcgcgggga acactctaaa cataacctat tattaattaa tgatttttta
      481 agccagtcac aatctaccaa ctttacagta tcacacaaac aacacatcca ttatgttaaa
      541 gagtacttaa ttcattgatt aaaaaggtaa atatttaaaa taactctata caaattaaaa
      601 tctaccaaac tttaccgcaa taattttcac tccagcgaaa aattaatgcc acagaatttg
      661 tagagaatgc taatgattag ccctggatgg gtaaaatgga ggagttttca gaggcgacaa
      721 acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct tataaatgtt
      781 taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat tacgcgcaat
      841 gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct gcagtgcaat
      901 gactcgcggt ttatcatcgg tcagcgttgc caatagttca tccagtgctt caatatcgcg
      961 tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg acaacacgtc
     1021 atagccgccg cgcatgttca gctttggcga tacggtaacc caggtattcg gtgtgcagcg
     1081 tacctcatga gtaccactgg tttcgatctg gcagctaaaa ccgttctttt cgagcagatc
     1141 agtcagtggt agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca catgccgcgc
     1201 ggtgtatccc tggcgactaa tgacagccaa caagtcttca ctgctcgccg ccccccactt
     1261 atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat cctcaagctt
     1321 ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc gggcatccct gtaaacgaat
     1381 aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga acatctcgtt
     1441 aatcgggtac tgcatagcat tctctgtgaa gtggataatt gttaattatt gcagatcctg
     1501 ccacaacaat catgtcttat taacattctg ttacaggcag atttaaagaa aaatgcgaaa
     1561 catatcgtta ataattaaag ggagtaacgt attatgtcag aagaaaataa agaaaatgga
     1621 tttaatcatg tcaaaacatt caccaaaatt atatttattt tttctgtatt agtttttaat
     1681 gataac
//
LOCUS       DAJTYZ010000311         1671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000311 DAJTYZ010000000
VERSION     DAJTYZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.316"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="OO596_004349"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="OO596_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HCR6719987.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..>1671
                     /gene="istB"
                     /locus_tag="OO596_004350"
     CDS             1253..>1671
                     /gene="istB"
                     /locus_tag="OO596_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCR6719988.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATL"
BASE COUNT          411 a          420 c          475 g          365 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac t
//
LOCUS       DAJTYZ010000312         1573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000312 DAJTYZ010000000
VERSION     DAJTYZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.317"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..156)
                     /gene="cas6e"
                     /locus_tag="OO596_004351"
     CDS             complement(<1..156)
                     /gene="cas6e"
                     /locus_tag="OO596_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6719989.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
     gene            complement(138..884)
                     /gene="cas5e"
                     /locus_tag="OO596_004352"
     CDS             complement(138..884)
                     /gene="cas5e"
                     /locus_tag="OO596_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCR6719990.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(895..>1573)
                     /gene="cas7e"
                     /locus_tag="OO596_004353"
     CDS             complement(895..>1573)
                     /gene="cas7e"
                     /locus_tag="OO596_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCR6719991.1"
                     /translation="FDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVDIAMFGRMLAN
                     KPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGETGFGSALFYT
                     YICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAYASWALAEKGT
                     DQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFDVMNKQGSMKD
                     VLDFICA"
BASE COUNT          376 a          433 c          388 g          376 t
ORIGIN      
        1 gagttcttcc cgacgataaa gaaattgcct ttctttgccg ccagggaaaa gatcccacag
       61 ccactgatgc atcacatatt caccgcgatc caccagatgc agcaactgcg caggcgaaag
      121 ctggccggta tgtaaggtta ttcttgagag gtacatggtt cctccttgct gagccacggc
      181 ccctgattga cggtgcgttc cccaaacagc cactgctgac gatttaaagg aacatcccgg
      241 cggcgtaata ttttgctcgc gaccaggccg tcgtgtttcc cttcccacca gcattcatcc
      301 tgaagtttcg ggagtgagac tttcagttta tggaaatgat cctgatactg ttggtatgca
      361 ttacataagg catcacacgc gttgccttcg agcagtaacg gcgcaagagg taatgccagc
      421 ggatgacttt ttcgccccag ataaagcgga aaaactggat ggcgtaaacc gtcctgcaac
      481 tgttcgaggc tgtaaggcgc atcggctgtt gttgccaccg ccaccatcca ccaggcatcg
      541 gtgtagtagt cgcgccggga gataatcgcg ctcaaaagct cagggtcgct caactcttca
      601 cgacggctga aataacgtgc tttacgcacc tcttttggca tctggaccgt gtgataatcc
      661 cgtgcccagc gcgggttacg gctggcgcaa accaccagtg aatagtggcg attaaacgcg
      721 tttaagcgtt cggtatcatc acgccgaatc cctaccccgg cagccagtag ccccagcaat
      781 gctgagcgcg aaggaagttc atgggtatga cgaacttcac ctggagcatc gacaccccag
      841 gacgccatcg gcccatgaag ctgaaaaatc agatattggc tcataagccg ccccttacgc
      901 gcagataaag tccagcacgt ccttcatgct tccctgtttg ttcatcacgt caaaacttgc
      961 gcattcggtc ttctgttcgt agaccgtatt catgttttcg cgaagcgttg taatacgctg
     1021 caccgccaca tctaactgac gtgtgccatt aatgggttca tagaaagccg ccgccagaga
     1081 gcgtggttga tcggtgcctt tttctgccag cgcccaggaa gcataggcac ggctggcaaa
     1141 gctattttgt ttaccggttg gggaaacttt aagcgcggct tccgtaaaag cgcgtatggt
     1201 ctgattagct aacgcttcgt caccgccgag gttttctacc agcagatctt tatcaatgca
     1261 gatataggtg tagaaaagtg ctgaaccgaa tccggtttct ccgagatgcc cggcaccggc
     1321 atcttcagag gcctggcgca aatcatcaac ggcggtgaaa aaatcatctt cgacaatcgt
     1381 ttcactgaca ccaaacgcat gcgcgacctg gcaggcggct tcaacattaa actcaggttt
     1441 attcgccagc atacgaccaa acatagcgat atctaccgct atacggtctt tacgtaacaa
     1501 cgcgagatct tcctcttttg gcgcgcgctt ttcttctgcc agttgatgag caagcgcttt
     1561 tacggcgtca aat
//
LOCUS       DAJTYZ010000313         1547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000313 DAJTYZ010000000
VERSION     DAJTYZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.318"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            442..1020
                     /locus_tag="OO596_004354"
     CDS             442..1020
                     /locus_tag="OO596_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719992.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1129..1387)
                     /locus_tag="OO596_004355"
                     /pseudo
     CDS             complement(1129..1387)
                     /locus_tag="OO596_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1371..1547
                     /locus_tag="OO596_004356"
                     /pseudo
     CDS             1371..1547
                     /locus_tag="OO596_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          496 a          272 c          347 g          432 t
ORIGIN      
        1 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
       61 cggtcaaaat gcttctgccg tgaaggaggg agaatgccat ctcatcactt atatctggcg
      121 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      181 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      241 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      301 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      361 aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc ctgttaatat
      421 ttaaggagaa tataaaataa aatgaaagtc tcgtttaagt ctttaggcta tatttttcat
      481 gatatatata ataaaaaaca cactatcgat gagttcaacg atgtggtaag aaaagcagtt
      541 ttatcaggta aaattaacga gctgaatgca tgtcataaag tggcaatttt tcttgccgaa
      601 aaagacaatg agataacaaa gaaagacaaa gccaagataa tagatacatt aactgaaaac
      661 tattccattg aatttcaaca attaatgaat attagtgagc gcacgttaaa ttcatcttta
      721 tatattactc ctggtgaaag tggttttgtc agttttgtta atcgtgaagg aaaaatatgt
      781 catactgcat atgtaaaatc atcagataat agcatggcat attaccatgc aaattatagc
      841 agcattgaca aatatattac ggatatgtgc ggccttattt gtatgaggca tattgagtct
      901 actggcatta ttttttatat gcttgatgaa aaagttttat ctgccattgc tgaatttatg
      961 aatgaaaaag gatggcgggc tgcgttttgt tcagcaaaga acttatataa atgtgtttaa
     1021 ttttagaaaa actatttggt aaaatccaac aagagtcgag aggttcgcgt tgcttcagca
     1081 gggcctcagc ctgttcaacc agactcagtt gcaggtcgtg acgcagattt agtggtggtg
     1141 gttgattgtc agataccagc cgatgatctt gtagtgcata tcatcagact ttgagaagca
     1201 tatggttttg cgggtaagcc gcttaagatg tgtccgcagg ttcagattat gtcgctctat
     1261 ccgctgagtg catttcttgc ttataacatg actggttgag gacaataaag ttcggtatac
     1321 cggctcaggc atcggtcatg tagaaggcaa tgctaaactg gctcagcaaa ctaagagcag
     1381 cgcgcatcca cttacccaat gcggttgaga aaatagcctt cgatcgcttt catgtggcga
     1441 aacaaccggg tgaggtggtg gataaaaccc gccagaatga acatccgcat cttcctgttg
     1501 aaagtcgtcg tcaggcaaag ggaactcgct tcctgtggca gcacagc
//
LOCUS       DAJTYZ010000314         1496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000314 DAJTYZ010000000
VERSION     DAJTYZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.319"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            210..1325
                     /locus_tag="OO596_004357"
                     /pseudo
     CDS             210..1325
                     /locus_tag="OO596_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          587 a          269 c          215 g          425 t
ORIGIN      
        1 atagttgtta acgatttcct ttacgatact atcactaccc tttttttata cacaaatatt
       61 accgggtgga gataaaaggg aaatcaaagg taattatatc aggaagatat aacgcattac
      121 atttattgtg tgtagaagaa aatccattcc tgttctaatt atccaattta aacatcttag
      181 cataaaacaa atgatgaata aggaatttta tgtcaattga ctttacccca ggtataataa
      241 atacatatca cggcgatatt tataactgca caacaaatac cgataatgct aaaacgccag
      301 acacaccgaa gtggccttgc gataattggg aagaacaaca acccattaat tccacattct
      361 ctggagaagg atatatctct gatcaatatg atttagtgca acaccaactt caacaaatta
      421 atgcgtgcca cacgaacact acatatacaa atgcagacta ctcaaaagtt gtggctcaac
      481 ttgtaagtct tattactaac attgaaacga taagttcgac acaacttacg caacagacac
      541 aatcaatact taatcagatt aataacatta gatatgaaaa aaacaaaagt gctgaatgtc
      601 gaatcattgt tgtcgctaat cctaaacccg acaaggcaat cataactaaa attagcgtag
      661 aagagggaat acccataaca ttcagtgtcc aaaccatgtt ttcagatact aattttattg
      721 ctgaacaacg agctgactta ccaactaata taaaagatat tcaaagcctg tatcaaaaaa
      781 tgacgaagtt atatattgag cacagtgaaa ataaaaacag gatgaaagtc tttgccggag
      841 ccaactttat tgatttcaat atgacaggac agaacctgtc cgggttcgtt ctaaccttgt
      901 caaggtttta tttcgaagat ctacttaata taaatttcac tgacgcaaat ttaggtgaca
      961 ctatttttta cataaagaac accctacccc caaattatat aaagatggac aatatcttga
     1021 caaacaaatc gaaggtttat tttcaacatt attaaccatt aatgacaact ttttacgagc
     1081 aaaagcggaa attgcttcga caataattaa atttttagag gccaggataa ccaatctttc
     1141 atacaatgac atattaaaat atcagcaaga attccaaaaa caatgttata agcaagttaa
     1201 agcctttacg acactctctc gatacaataa aattcaaaca tgggcagaaa tgtctgaata
     1261 tcagttcgaa gtatttcaat acgagacgct taaccccaaa aaaatgtcac acacgcctta
     1321 tttaaagagg ccacttccta atgaaaaaga tataaattat ggggtggaaa tagaaatacc
     1381 atctggcaaa agaattcgct tatcaaatca ttatcagaat ataataccat aatattgatt
     1441 attagcactt tataattatt gaatgaaaat agattttatg taattttaaa acaaag
//
LOCUS       DAJTYZ010000315         1485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000315 DAJTYZ010000000
VERSION     DAJTYZ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.320"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..92)
                     /locus_tag="OO596_004358"
     CDS             complement(<1..92)
                     /locus_tag="OO596_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6719993.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKL"
     gene            complement(164..>1485)
                     /gene="xylA"
                     /locus_tag="OO596_004359"
     CDS             complement(164..>1485)
                     /gene="xylA"
                     /locus_tag="OO596_004359"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCR6719994.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          357 a          414 c          336 g          378 t
ORIGIN      
        1 agcttttccg tttgcgaatc aaccacctca ccctgctcgt tgagcaaaat aacttttacg
       61 cccgaggtgc caagatctat cccgatatac atatcgatcg ttccttaaaa aaatgcccgg
      121 tatcgctacc gataaccggg ccaacggact gcacagttag ccgttatttg tcgaacagat
      181 aatgatttac cagattttcc agttgctcct ggcgaccact ctgatgcacc ggagacaaat
      241 tatgttcctg agcatatttg gctaaatctg ccagtgacat ttggcctttc aggatttgct
      301 ggcccaattc gctattccag ccggaataac gctgcgcgat gcgtttatcc agctcgccat
      361 cttcaatcat gcgcgctgca attttcagcg ccagtgccat cgtatccatc gcgccgatat
      421 gaccgtaaaa cagatcatat ttatcagtac tttgacgacg tactttggca tcgaagttca
      481 gaccaccggt ggtgaaaccg cctgctttga gaatttcata catcaccagc gcattctctt
      541 ccacactgtt cgggaactgg tcggtgtccc agcccagttg cgcatcgcca cggttggcgt
      601 cgacagaacc gaacaggcca agcgcaatgg cggtggctat ttcatgatgg aaagagtgac
      661 ctgccagcgt cgcgtggtta gcttcaatgt tcagtttaat ctctttttcc agaccaaact
      721 gtttcaggaa gccatagacc gtcgcggcat cgtaatcata ttgatgttta gtcggttctt
      781 gcggtttcgg ttcgataagc aacgtgccct ggaagccgat tttatgttta tgctcaacca
      841 ccatctgcat aaagcggccc agttgttcac gctcctgacg caagtcggta tttaacagcg
      901 tttcgtaacc ttcacgacca ccccacagga catagttttc accgcccaat ttatgggttg
      961 cttccatcgc tgtaacaact tgcgttgccg cccagctgaa gacttcagga tctgggttcg
     1021 tcgccgcacc cgcgccatag cgagggtttg taaagcagtt agcggttccc cacagcagct
     1081 tcacgccgct ctcttcttgc ttggctgcca ggacatcaac catttgcgca aaattattga
     1141 tgtactcttt taacgacgcg ccctcagggg aaacatccac atcgtggaag caataaaatg
     1201 gcacatgtaa cttgtggaaa aactcaaatg cgacatctgc tttacgcttc gccaacgcca
     1261 gtgcctcacc aggctgctgc cacggacgat taaacgcccc cacaccaaac atgtccgccc
     1321 cgttccagca gaaggtgtgc cagtagcagg cggcaaaacg caagtgctct tccatacgct
     1381 tacccaacac cagttcgtcg ggattgtagt gacggaatgc taacgggttt gaggattttg
     1441 agccttcata acgaacgcga tcgagctggt caaaataggc ttgca
//
LOCUS       DAJTYZ010000316         1464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000316 DAJTYZ010000000
VERSION     DAJTYZ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.321"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1464
                     /locus_tag="OO596_004360"
     rRNA            <1..>1464
                     /locus_tag="OO596_004360"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          365 a          328 c          460 g          311 t
ORIGIN      
        1 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
       61 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcaaca
      121 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      181 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      241 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagtcccttg
      301 cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      361 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      421 taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
      481 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
      541 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
      601 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
      661 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
      721 gcatggagcc ggccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgtg
      781 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
      841 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
      901 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
      961 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
     1021 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
     1081 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
     1141 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg
     1201 agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc
     1261 gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag
     1321 catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag
     1381 gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct
     1441 aaccggtact aatgaaccgt gagg
//
LOCUS       DAJTYZ010000317         1452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000317 DAJTYZ010000000
VERSION     DAJTYZ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.322"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..762)
                     /locus_tag="OO596_004361"
     CDS             complement(<1..762)
                     /locus_tag="OO596_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6719995.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPL"
     gene            complement(1363..>1452)
                     /locus_tag="OO596_004362"
     CDS             complement(1363..>1452)
                     /locus_tag="OO596_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6719996.1"
                     /translation="RTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          388 a          235 c          346 g          483 t
ORIGIN      
        1 caatgggtta tcactaatat ctattgaaca ttcattcctc agattaagaa tgctttccgg
       61 gatatgactt atctgattta tgtcaaaata accgttcctt aaattttccg gcaggtatgg
      121 aatatcggtt aacatattgt cgattgccca tatggagtga atgttgtgag gtagaggggg
      181 gagttcacta agaagattcc ctattgcgct aatctcttgt aaagaaaatg gtaaaggtgg
      241 taaaactgcc agcccattac atgatacatc taatgtttcc agtagttctg gtaaaacagg
      301 aagagaacat aattgattat ttgagacatg aagctccttc aaagatatgg ggagtgtggg
      361 tagtgtgatt agttgattgt gggacgcatt caataattta agtccttgag gcaaagcagg
      421 cagttcaata agtctgttat agctgacatt aagctgtgta agggacgcag gcaatgggga
      481 gattaagctt aaattatttt tacttatatt aattgattta atttccgggg ggatttcagg
      541 taatgttgtc aggcctaatt cagacaagtc caggttcgtc tcttggtttt gtagacatga
      601 tactagtcgc tgaaaagcga tgtctcgttg ttcttcttgt atgcggttat ttttccattc
      661 agtccaatgg gttagataat tttcatatgc acggctagtg tcgattgaat aagtggagaa
      721 cgaatttgaa attaatctgt gattgttatt tgtcgggagc ataaatatca gggcgtattc
      781 tttatgttga gaggtgttgg attctttttt atctgctttg caatgttatt ggttcccttc
      841 agcagtagac aggaacttct ggaggaggcg aatatatagt ttgtgtaagc atattaactc
      901 catatgttat atattgaaga acttctgctt tacactatcc taaactggat cggtttaatg
      961 taaaaaaacg atactttttg ggggaggggg caatttttcc ttaaggtatg aaaaaaggag
     1021 ctgaagctat ataatagcta ttaatgccac tggtatcagt gaatcatgta tataaaaaac
     1081 tgtgttaagg aggctgtcca ttagcggttt tttattcttc tggaaccgcg gcatctttgc
     1141 gtatcttttt gtcttctcac cgtctggtcg gtgttttgct gagactgcta atttcctgtc
     1201 tttgctagtg ctgtactggt actgcccaat catgattggc gggggcgggc ggagttgaga
     1261 ccgcagacac gtcgtatgca agaacgtgct gcggctggct ggtgaacttc cgatagtgcg
     1321 agtattgaat gatttccagc cgttactgat tttacgtgct aattagtgaa caaaccactc
     1381 gtcagcagac tcccaggtat ctttcagagt ttcctgaaca aaagttttag ctgaatcttt
     1441 atcggcggtg cg
//
LOCUS       DAJTYZ010000318         1439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000318 DAJTYZ010000000
VERSION     DAJTYZ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.323"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..263)
                     /locus_tag="OO596_004363"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OO596_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO596_004364"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO596_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6719997.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1439)
                     /locus_tag="OO596_004365"
                     /pseudo
     CDS             complement(1175..1439)
                     /locus_tag="OO596_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          409 a          357 c          250 g          423 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttactcttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagtg ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcga gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg gctaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacgc ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctg
//
LOCUS       DAJTYZ010000319         1428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000319 DAJTYZ010000000
VERSION     DAJTYZ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.324"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..118)
                     /locus_tag="OO596_004366"
     CDS             complement(<1..118)
                     /locus_tag="OO596_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6719998.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="OO596_004367"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="OO596_004367"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6719999.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1428)
                     /locus_tag="OO596_004368"
     CDS             complement(822..>1428)
                     /locus_tag="OO596_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6720000.1"
                     /translation="KGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFC
                     FGKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGAD
                     EEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVP
                     GAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          324 a          397 c          396 g          311 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgccttta
//
LOCUS       DAJTYZ010000320         1427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000320 DAJTYZ010000000
VERSION     DAJTYZ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.325"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            21..434
                     /locus_tag="OO596_004369"
     CDS             21..434
                     /locus_tag="OO596_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6720001.1"
                     /translation="MHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIK
                     LFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPF
                     ETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            459..>1427
                     /locus_tag="OO596_004370"
     CDS             459..>1427
                     /locus_tag="OO596_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6720002.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLD"
BASE COUNT          386 a          330 c          340 g          371 t
ORIGIN      
        1 aagaaaaaat attacgcatt attcacacca aaggtgtgtc attgcctgat gatcgcgagt
       61 cagtcttctg gctgaatatt aaaaatattc cgccatcagc gagcaacaaa gcgacgaact
      121 cgctggaaat agcagtaaaa acgcgtatca agctgttctg gcgaccagca aatatccgcc
      181 ttattcccga agatgctgcg ccgaaagtga aatggcgccg ggaagggcga aatctgatag
      241 cagagaatcc caacccaatt catatcagtg tgatggatgt catcgttgat ggccatgatg
      301 tgcccctcaa tatgatccgt ccttttgaaa cgctcacgtt acctttacct gctaattctg
      361 cgggaggcca aatgacctgg cgatttatta atgattacgg cgcagtcagt gacccgataa
      421 aaatgacgct gtaagcaagg gataaggata ggcgtattat gatgacgacc agaatagtgg
      481 ttggcctcac ggcagggacg tgtctgattt tctcgcaaaa cctgatggcc gaggtcagtg
      541 tattcaatcc ggcgcttctg gaaatcgacc atcaatccgg agtcgatatt cgccagttta
      601 atcgggcaaa cctgatgccc ccaggtgttt atagcgttga tatttttatc aacggtaaaa
      661 tgtttgaacg tcaggatgtg acatttgttc aggataatcc agatgctgat ctgcacgctt
      721 gctttattgc cattaaaaaa acactgtcct cctttggcat aaaagttgat gcgctcaaat
      781 cgttcaatga tgtggatgag acggtttgcc tcgatcctgc tccacgtatt gaaggctcat
      841 cctggcagtt tgacagtgat aaattgcagc tgaatatatc cattccccaa atctacatgg
      901 acgcgatggc ttatgattac atcagcccca cgcgttggga tgaggggatt aacgcgctca
      961 ccatcaacta cgatttttct ggttcacata cactacgttc agattatggt tcacaagaga
     1021 cagataccag ttatctcaat ctgcgcaatg gactgaatat tggaccgtgg cggctacgta
     1081 attacagtac tttaaacacc agcgatggcc gtgcggaata caactccatt agtacctgga
     1141 tacagcgcga tattgccgcg ttaagaagcc agattatgat tggtgatacg tggacggcga
     1201 gcgatatttt cgacagtacg caaattcgcg gcgcgcgttt gtatactgat aacgatatgc
     1261 tacccgccag ccagaatggc tttgctcctg tggttcgtgg gattgcaaag tccaacgcca
     1321 ccgtcatcat tcggcagaat ggctacgtga tttatcagtc tgccgttcca caaggtgctt
     1381 ttgagatcac cgatctcaac accgccagta caggtggcga tttggac
//
LOCUS       DAJTYZ010000321         1412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000321 DAJTYZ010000000
VERSION     DAJTYZ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.326"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..477
                     /gene="istB"
                     /locus_tag="OO596_004371"
     CDS             <1..477
                     /gene="istB"
                     /locus_tag="OO596_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HCR6720003.1"
                     /translation="HYIIHRKNLLITGPTGCGKSWIANALGEQACRQKYSVRYCRTGR
                     LLEQLAQGRVDGSWLKYLKQLQKIQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSST
                     IVVSQFPVDKWHGLMENPTTADAILDRLVHNSHRVVLQGESLRKNPPTVESSEKTS"
     gene            835..>1412
                     /locus_tag="OO596_004372"
     CDS             835..>1412
                     /locus_tag="OO596_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HCR6720004.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT          366 a          380 c          366 g          300 t
ORIGIN      
        1 cactacataa tccacaggaa aaacctactg ataaccggtc cgacaggatg cggtaagagc
       61 tggatagcca atgcgctggg cgaacaggca tgtcgacaga aatacagcgt ccggtactgt
      121 agaaccgggc gattactgga acagttggct cagggacgtg ttgatggcag ttggctgaaa
      181 tatctgaaac agttgcaaaa aatacaggtt ctaatcctgg acgatctggg gctggaacag
      241 ttaagtaacg cccagtgtaa tgaccttctg gagatcacag aggatcgtta cggacaaagc
      301 agcacgatag tggtaagcca gttcccggta gataaatggc atggtctgat ggagaaccca
      361 acaacagcag atgccatcct ggacagactg gtacataact cacacagagt ggtccttcag
      421 ggagagtccc tgaggaaaaa tcccccgaca gtggaaagca gcgaaaaaac gagttaaaaa
      481 gcggtagaaa aagttaagtt cccggttgat cgaatggatg cgaatcgctg atcgaacttc
      541 gatgccaatc cgtgatcgaa tagagcggaa tacgcagact accccccttt atatataccc
      601 ctaccccgtt actttattcg taccccttat aatggggtgt tagccagcca gacccggcat
      661 gattactgcc cccagtcgtc catgatccgg ggggtgatgt caccgggtct ggtggggcgc
      721 tggtaaccgc taataggggt caggtcaggc acttttgccg ggaccgtctg taacgtggat
      781 gccggtacct gctccccgtg gttatctggt taacccatat acaagggaga cagaatgacc
      841 gaatccagcg attacgaatc cgtccaggtc tttatcggcg ttgatgtcgg taaagatacg
      901 catcacgctg ttgccattaa tcgttcaggt aaacgcctgt tcgataaagc attacccaac
      961 gacgaaaaca aactcaggtc gctaatatct gacctgaaac aacatggtca gatactgctg
     1021 gttgttgatc agccagctac catcggtgcg ttacctgtcg ccgttgcccg ctcagaagga
     1081 gtccttgtcg gatacctccc tggactggcc atgcgccgca tagccgactt acacgccggt
     1141 gaagctaaaa ctgatgctcg tgacgctgcc atcattgccg aagctgcccg taccctgcct
     1201 cacgcgctac gcacgctgaa actggctgac gagcaaatcg ccgaactctc catgctctgc
     1261 ggcttcgatg atgatcttgc cgcacagaca acgcaggcca gcaaccgtat ccgcggcctt
     1321 ctgacccaga tacatccggc actggagcgc gttctcggtc cgagacttga gcacccggcg
     1381 gtactcgatc ttctccagcg atatccctca cc
//
LOCUS       DAJTYZ010000322         1410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000322 DAJTYZ010000000
VERSION     DAJTYZ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.327"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1410
                     /gene="rhsC"
                     /locus_tag="OO596_004373"
     CDS             <1..>1410
                     /gene="rhsC"
                     /locus_tag="OO596_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HCR6720005.1"
                     /translation="TQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAV
                     WLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHT
                     GRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRV
                     VKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYY
                     NHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATEDATG
                     SRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAV
                     KDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGR
                     VIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYD
                     EADRLTHRTVNGETAERWQ"
BASE COUNT          336 a          419 c          433 g          222 t
ORIGIN      
        1 cacgcaggcg cagcgggcag aagaagcccg gcagcaggcc atttccggcg ggacggaacc
       61 gtccgctttt cctgataccc tgccgggtta caccgaatat ggccgggaca acggcatccg
      121 tctgtctgcc gtgtggctga cgcacgaccc ggaatacccg gagaatttac ctgccgcgcc
      181 gctggtgcgc tatggctgga cgccgcgcgg cgaactggcg gcagtgtatg accgcagcaa
      241 tacacaggtg cgcagcttta cttacgatga taaataccgg ggccggatgg tggcgcaccg
      301 tcacacgggc cggccggaaa tccgttaccg ttacgacagc gacgggcggg tgacagaaca
      361 gctaaacccg gcaggcttaa gctacacgta tcagtatgag aaggaccgca tcaccatcac
      421 cgacagcctg aaccgccgtg aagtgctgca cacgcagggt gaagccgggc tgaagcgggt
      481 ggtgaaaaag gaacacgcgg acggcagcgt cacgcagagt cagtttgatg cggtgggcag
      541 gctcagggca cagacggatg ccgcaggccg gacgactgag tacagcccgg atgtggtgac
      601 gggactcatc acgcgcatca ccacgccgga tggcagggca tcggcgtttt actataacca
      661 ccacagccag ttaacgtcag ccaccgggcc tgacgggctg gaaatgcgcc gcaaatatga
      721 tgaatccggc cgtctgattc aggaaactgc ccctgacggc gatatcatcc gctaccgtta
      781 tgataatcca cacagtgact taccctgcgc aacggaagat gccaccggca gccggaaaac
      841 catgacgtgg agccgttacg gtcagttgct gagcttcacc gactgttccg gttatgtaac
      901 ccgttatgac catgaccgct tcgggcagat gacggcggtg caccgcgagg aagggctgag
      961 tcagtaccgc gcatacgaca gccgtggaca gttaattgcc gtgaaagaca cgcagggcca
     1021 tgaaacgcgg tatgaataca acgccgccgg tgacctgacc accgtcattg ccccggacgg
     1081 cagcagaaac gggacacagt acgatgcgtg gggaaaagct atctgtacca cgcagggcgg
     1141 tctgacgcgc agtatggaat acgatgctgc cggacgggtc atccgcctga ccagtgaaaa
     1201 cggcagccac accaccttcc gttacgatgt actcgacctg ctgatacagg aaaccggctt
     1261 tgacggccgc acacagcgtt atcaccacga cctgaccggc aaacttatcc gcagcgagga
     1321 tgaggggctg gtcacccact ggcactatga cgaagcagac cgcctcacgc accgcaccgt
     1381 gaatggcgaa accgcagagc ggtggcagta
//
LOCUS       DAJTYZ010000323         1403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000323 DAJTYZ010000000
VERSION     DAJTYZ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.328"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(338..604)
                     /locus_tag="OO596_004374"
     CDS             complement(338..604)
                     /locus_tag="OO596_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720006.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
     gene            complement(786..1184)
                     /locus_tag="OO596_004375"
                     /pseudo
     CDS             complement(786..1184)
                     /locus_tag="OO596_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          361 a          286 c          293 g          463 t
ORIGIN      
        1 agccgtctat gggaaggagt ctgctgttat tccagagaat ggtaattcag tcatatattt
       61 ctccatctat gacttgtcaa taccatcctg atatttgtgg gtcagcaata tattctacta
      121 aaaggtattt aagatcattg cgtccctgca ctcgaataat ggtgaagaaa gcatgatgaa
      181 tatatcacga taacgttcag tatgtatcga ggatagatgg agttactcct ctctgaaata
      241 gaattactgc tctacttttt ctgtttccag aaattactag taacaaaccc tgatatattc
      301 ttttgaaaat ggagcttaaa cgagtctgaa attaacttca gatattagaa tgccgttggc
      361 tttgtttttc aacaattcct acttcattta tctttgtcaa ccattgcatt tctgtagcag
      421 tgaatttcaa cgttttcgtc cggttggcag caggtatttg taattcgtat atagctgctt
      481 ttttattgaa aacagtatat ccatctgtag taacagtaga agataataat acaggatttg
      541 atacttcaag gatgatattt tcttgttttt gtggaaggca agggaatata gtttgtgtaa
      601 gcatattgct tccttatgtt atattttagt aaagaattta ttttttacat tatagtcggc
      661 taaatgaagt ttactgtaaa aaaacgatac ttttttcgta agcgatgaga tgtggagcca
      721 gaaaaatggc tgcgttacgt cattgaactg tccttgaccc acgatattca gacagtcgtt
      781 gtatcttaag ttgacattac ccgctgacgt cgatattccc ttggtgagag ataacccaac
      841 gcactatgcg gatggttttc attgtaatgc tcgatcgcca ctgcaaggtt atgcaatgcc
      901 gttcttggtt tcggcatgaa cgcgatgcag tcttccttca tcgttttcac gaaccgttct
      961 gctattccat tactctgagg gctactgact gccgtcgtgc agggctccag attcaactct
     1021 ctggcgaact gccgtgtttc atgagccgta taagccgaac cgttatcggc cactggattg
     1081 gccgttccgg tcctttatcg ccatatcgct gacatgacat cctgcaccgt tgctttgtca
     1141 taatctcccg tgcctgctgc ccagtctatg gcttcacgat cgcaatatgg aacgtaacgc
     1201 tgtcatcggg agaatcgtca atgtggcatt cggttaacgc tgatatgact ttccgtgtca
     1261 aaccggaatc tctaattttg acgattttct ttttaggttt aattccctga gggcgacgta
     1321 gcagtaaatc cgacggtgaa tcacgctgat attcgcgggt tggataccgc attacagagg
     1381 tccgtcatat gagcctgcca ctt
//
LOCUS       DAJTYZ010000324         1387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000324 DAJTYZ010000000
VERSION     DAJTYZ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.329"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(56..1387)
                     /gene="xylB"
                     /locus_tag="OO596_004376"
                     /pseudo
     CDS             complement(56..1387)
                     /gene="xylB"
                     /locus_tag="OO596_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="xylulokinase"
BASE COUNT          331 a          423 c          325 g          308 t
ORIGIN      
        1 tagtgagata tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg
       61 ccattaatgg cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag
      121 cggcataacg ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact
      181 caatgagcga tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg
      241 gccccacatc ccccccccgt acggtaatcg agcttctgac cgctgatatc cgccagcatc
      301 tgacgccagt actcactacg cgccccgcca ccaatcaacg taacactttg cggtttaatg
      361 ccgcaggcat gcacgacatc catgccatct gccagcgcat aacccacgcc ttccagcact
      421 gctcgcgcca gttcattggg gccatgttga tgagtcaaac caaagaaaac ccccttcgcc
      481 tggggattat tgtgtggtgt acgctcgccg gaaagataag gcagaaacca aactggctcg
      541 acactttcat cagcctgttg agctgcagcg attaaagctg ggacattgca caggccggtt
      601 aatttcgcgg cccaatccag acacgatgct gcactcagca ttacagacat taaatgccaa
      661 cgctgcggta gcgcatggca aaagctgtgt acagcgcttt ctggcttgct taagaaccct
      721 tcgctgacag caaaatagac ccccgacgtc cccagcgata acattgcctg attagcatca
      781 accattccca caccaactgc accagctgca ttgtcgccac cgcctgcgac aactggcacc
      841 gtcgccatac cccacgcttt cgcaacttca ggtaacaaag caccagtaat ttcgctgcct
      901 tcgtataatg cgggcatctg gtcacgagat aagtcgcaag cctgcagcat gacgtcactc
      961 cagtcacgct ttgcgacatc cagccacatg gtgccagctg cgtcagacat atcgctggca
     1021 aactcccccg tcatacgcag acgcaagtaa tctttcggta ataatacttt gtcgatttga
     1081 cggaatatct ccggctcatg ccgctgaacc catagcaatt taggcgcagt aaatccgggc
     1141 atcatcaggt tgccggtaat cactcgtgat tgcggaactc tcgcttccag caaagtgcac
     1201 tcttgcgcac agcgcccgtc gttccacaaa atggcagggc gcaatacccg ttgttgagca
     1261 tccagtaagg ttgctccatg catctggccg gcaataccca atgctttaac gtcctgcaga
     1321 gaatgctgat cgcccagagc tttcattgcg cgatcagttg cctgccacca ctgttccggg
     1381 tcttgtt
//
LOCUS       DAJTYZ010000325         1372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000325 DAJTYZ010000000
VERSION     DAJTYZ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.330"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            522..>1372
                     /locus_tag="OO596_004377"
     CDS             522..>1372
                     /locus_tag="OO596_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6720007.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTT"
BASE COUNT          316 a          347 c          386 g          323 t
ORIGIN      
        1 atccgctccg gtgggccttt ttactgcaga aaacaggttc cccgttaaat gctatgttgc
       61 tcacaattca gtaagttgac agttgcctgt cagactgggc atttgttaaa aaaatttcgc
      121 atggtgaatc cccctgagcg gaggggcgac tggtgacggt ataatctctg attatcaaaa
      181 cgagaatgac gcgggtttag tggcaccggg ctgaactcac cgggaggcac ccggcaccat
      241 gtgcatgatg atacagatac gcggctttag cccctctccg gaggggtttt cttgtgggca
      301 aaaaaagccc gcgctgggag acgcgggcgg caaggaataa acaataaaac gtgaagtaat
      361 atttcagctg gcgaataata ccccatagta atcactctgc gcaactgcgc ggtctttttc
      421 gaattgcggg ctgtcgtctc tcttctgcca ttgtcctgta acttccggac ttcagcccgc
      481 tccttatttt actcacaata ttatcccggc cgggaggatt catggcattt aaacactatg
      541 acgtggtcag ggcggcatcg ccgtcagacc ttgcgaaacg actgacacaa aaactgaagg
      601 agggctggca gccgtttggt agtccggtgg ccataacccc ttataccctg atgcaggcga
      661 ttgcagcaga aggtgatgtg gtcgtcagtg gtgcaactga gccggagtgg tactacgtca
      721 tcgtactggc cgggcaatcc aatgccatgg cttacggtga aggtcttccg cttccggatt
      781 catacgatgc gccccatccg cgcattaagc aactggcccg tcgtaacaca gtgactcccg
      841 gtggtgaagt atgcgtattt aacgacatca ttcctgctga ccattgtctg catgatgttc
      901 aggatatgag tacgattaac catccccggg ctgacctgag caaagggcag tacggctgtg
      961 tcggacaggg cttacatatt gccaaaaaac tgcttccgta tatccctaat aatgcgggga
     1021 tcctgctggt accatgctgt cgtggtggtt cggcattcac ccagggcacg gaggggacat
     1081 tcagcgagtc cacgggggcc agtcaggatt cggctcgctg gggagtgggt aagccgttat
     1141 atcaggatct gcttttccgc acgaaggcag cattgcagaa aaacccgaaa aacgttttgc
     1201 tggcgatatg ctggatgcag ggggaattcg atatgacgaa tgccagttac gcccagcagc
     1261 cagcagcatt tcttgcaatg gtacagcagt tccgtgctga ccttgccggg ctggcggcgc
     1321 agtgtcacgg tggaagtccg gcatcagtcc cctggatttg tggcgacacg ac
//
LOCUS       DAJTYZ010000326         1369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000326 DAJTYZ010000000
VERSION     DAJTYZ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.331"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            3..983
                     /locus_tag="OO596_004378"
     CDS             3..983
                     /locus_tag="OO596_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR6720008.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1369
                     /locus_tag="OO596_004379"
     CDS             998..>1369
                     /locus_tag="OO596_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6720009.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVL"
BASE COUNT          357 a          315 c          360 g          337 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctg
//
LOCUS       DAJTYZ010000327         1355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000327 DAJTYZ010000000
VERSION     DAJTYZ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.332"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            89..>1355
                     /gene="istA"
                     /locus_tag="OO596_004380"
     CDS             89..>1355
                     /gene="istA"
                     /locus_tag="OO596_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HCR6720010.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVV"
BASE COUNT          345 a          377 c          345 g          288 t
ORIGIN      
        1 ggaattaact cttttcacca cagtctccat tgccgtgttt aaaccagtgg agacgtcaat
       61 gtccaaaaag agaaaatcca ggagcactat ggaaacttgt tttaagatcc ttcagctcaa
      121 gttcgataag aagctgacta accgttgtat cggactgacg ttacacatca gcgcctcaac
      181 cgtttttgag gttcttgccc gcttcaaagc ctcttctctg tcatggccat tacctgcgga
      241 tatatcccat gacacgctgg agaaactcat tttcccgccc aaagacacct ctgcgtcaga
      301 gcttgttatg cctgacatgc tgtacttcga tacggaaatg agaaaacctg gcgtgacgcg
      361 ccaactgttg tggatggaat acaaggcaca ggcgggtgac aaagcgatgg gatactcaca
      421 cttctgccgt tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc gacaggagca
      481 tcgtgctggc gaaaagctct ttatcgactt ctgtggtcct acggtccctg ttattaatcc
      541 ggacaccgga gagatacgcc gcgtggcaat cttcgtggct gtaatggggg cgtccaacta
      601 tacctatgtt gaagcgtgcg aaggtcagga catgatgtca tggttaaatg cccacagccg
      661 ttgcctgacc ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc tcagaagcgc
      721 agtgaaaaaa gccgatcgct atgagcctgt catcaacgac agttatcagg cgctggctga
      781 gcactatggc acggttatta tcccggcccg cccgcgcaaa cctaaagata aaccaaaagc
      841 agaaaacggc gtcctcatcg tcgagcgatg gcttcttgcc cgcatccgta atgaaacctt
      901 ccacacgctc agggcgctga atgcacgcct tcgggagttg ctgacggaca tgaataaccg
      961 accaatgaag ggatacggta atcaaacccg cgcagaacgc ttccgcatgc tggatgcacc
     1021 agcactctct ccgctgcctc tggagcctta tgaatacacg gaatacaaag ccgtgaaggt
     1081 tggccctgac taccacgttg aatatgcccg tcactggtac tccgttccac acgaactggt
     1141 cggtcagcgt ctgtcactga aggtggggca gtcggtggta cagctctggc acaaggggca
     1201 gtgtgtggcg caacatccgc gcagcacaca tgaatataaa cacacaacca atccgcttca
     1261 catgccagaa cgccaccggc ggcatgggac atggacaccg gaacgcctta tcgagcaggg
     1321 aaaccgtaca ggtccgtcca caggccgggt ggttg
//
LOCUS       DAJTYZ010000328         1343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000328 DAJTYZ010000000
VERSION     DAJTYZ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.333"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..186
                     /locus_tag="OO596_004381"
     CDS             <1..186
                     /locus_tag="OO596_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720011.1"
                     /translation="ATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLA
                     QGKRRCQQQFTSQEVQN"
     gene            196..>1343
                     /gene="yicI"
                     /locus_tag="OO596_004382"
     CDS             196..>1343
                     /gene="yicI"
                     /locus_tag="OO596_004382"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6720012.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          319 c          352 g          337 t
ORIGIN      
        1 gccacgatta gcatcattat tgcgctattc acgattgttc cggcgatctg ttatttgctg
       61 agcgcgatta tcgctaaacg ctactactca ctcacgacgc acaatctgaa aaccgttatg
      121 gaacagctgg ctcagggtaa acgccgttgc cagcaacaat tcacctctca agaagtgcag
      181 aactaaggaa cggcaatgaa aattagcgat ggaaactggt tgattcaacc tggcctcaat
      241 ttgattcacc cgcttcaggt gttcgaggtt gaacagcagg ataatgaaat ggtggtctat
      301 gctgcccccc gtgatgtgcg tgaacgtacc tggcagcttg atacgccttt atttacgctg
      361 cgctttttct ccccacagga aggtattgtc ggtgtgcgga ttgagcattt tcagggggcg
      421 ctgaataacg gtcctcatta tccgctcaat attttgcagg acgtgaaggt cacaatcgaa
      481 aacacagaac gttatgctga gtttaaaagt ggcaacttaa gcgcgcgtgt cagcaaaggt
      541 gagttctggt cactggattt tctgcgcaac ggcgaacgta ttaccggtag tcaggtgaaa
      601 aataatggct acgtgcagga cacgaataat caacgcaatt atatgtttga gcggcttgat
      661 cttggcgttg gcgaaacagt ttacggtctg ggagagcgct ttactgccct ggtgcgcaat
      721 ggccagacgg tagagacctg gaaccgggac ggcggcacaa gtactgaaca ggcgtataaa
      781 aatatcccgt tctacatgac taaccgtggt tatggggtac tggtcaatca tccccagtgt
      841 gtctcttttg aagtgggatc ggagaaagtc tccaaagtgc agttcagcgt tgagagtgaa
      901 tatctcgaat actttgttat cgacggcccg acgccgaaag cggtacttga tcgttatacc
      961 cgctttactg gtcgtccggc gctgccgccc gcgtggtcct tcggcctgtg gctaaccact
     1021 tcatttacca ccaactacga cgaagcgacg gtaaacagct ttatcgatgg tatggcggaa
     1081 cgcaatctgc cgctgcatgt tttccacttt gactgtttct ggatgaaagc cttccagtgg
     1141 tgcgattttg agtgggaccc gctgactttc cctgacccgg aagggatgat ccgccgcctg
     1201 aaagcgaaag ggctgaaaat ctgcgtctgg attaacccct atatcggtca aaaatccccc
     1261 gtctttaaag agttacaaga gaaaggctat ttactcaaac gcccggacgg ttcgctatgg
     1321 cagtgggata aatggcagcc agg
//
LOCUS       DAJTYZ010000329         1342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000329 DAJTYZ010000000
VERSION     DAJTYZ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.334"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            114..368
                     /gene="bssS"
                     /locus_tag="OO596_004383"
     CDS             114..368
                     /gene="bssS"
                     /locus_tag="OO596_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6720013.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            483..>1342
                     /gene="solA"
                     /locus_tag="OO596_004384"
     CDS             483..>1342
                     /gene="solA"
                     /locus_tag="OO596_004384"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6720014.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGS"
BASE COUNT          358 a          331 c          338 g          315 t
ORIGIN      
        1 ccctttaaaa attcggtgaa tacccttact tattggtata ctgaagttgc ttcagataaa
       61 aacgcattga acctcgaata acgttgtcta gtaacacgaa ttagggggcc atgatggaaa
      121 aaaataatga agtcattcag actcatccgc tcgtagggtg ggacatcagc accgttgata
      181 gctatgatgc gctgatgttg cgtttgcact accagacccc aaataagtcc gagcaggaag
      241 ggactgaagt tggtcaaacg ctctggttaa ccactgatgt tgccagacaa tttatttcga
      301 tattagaagc aggaatcgcc aaaattgaat ccggtgattt ccaggtaaac gagtatcggc
      361 gtcattaaca tgacatgacc atccacccaa aaggcacctc accggtgcct ttaccatttc
      421 tgggttgtat atctgcattc agttaattac tctgcttaaa ccatgagagc agagagaaaa
      481 aaatgaaata cgatctcatc attattggca gcggttccgt aggcgctgcc gccgggtatt
      541 atgcaacccg cgccggttta aacgtgctaa tgaccgacgc ccatatgcca ccgcatcaac
      601 acggcagcca ccacggcgat acgcgattaa ttcgccatgc ttatggtgaa ggcgaaaagt
      661 atgtcccgct ggtcctccgc gcgcaaacgc tgtgggatga actttcccgc cacaacgaag
      721 atgatcccat ttttgtacgc tctggtgtca ttaacctcgg cccggctgac tccgcatttc
      781 tcgccaacgt cgcccacagc gccgaacagt ggcaactcaa cgttgaaaag ctcgatgcgc
      841 aagggattat ggcccgctgg ccagaaatac gcgtcccgga caactacatc ggcttatttg
      901 agactgattc cggttttttg cgcagcgaac tggcgattaa aacctggatc caactggcga
      961 aggaagcggg ctgtgcgcaa ctgttcaact gcccggtcac cgcaattcgt catgacgatg
     1021 atggcgtaac tattgaaacg gttgacggtg agtatcaggc gaaaaaagcg attgtctgcg
     1081 cgggaacatg ggtaaaagac ctgctcccgg agctgcctgt ccagcccgta cgtaaagtat
     1141 ttgcctggta tcaggccgat ggccgctata gcgtgaagaa taaattcccg gcgtttaccg
     1201 gtgaactgcc caatggcgat caatattatg gttttccggc agaaaacgac gcgttgaaga
     1261 ttggcaaaca taacggaggc caggttatcc attcagcgga tgaacgtgtt ccgtttgcgg
     1321 aagtggtcag cgatggttcg ga
//
LOCUS       DAJTYZ010000330         1311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000330 DAJTYZ010000000
VERSION     DAJTYZ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.335"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..103)
                     /locus_tag="OO596_004385"
     CDS             complement(<1..103)
                     /locus_tag="OO596_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6720015.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVS"
     gene            196..579
                     /gene="crcB"
                     /locus_tag="OO596_004386"
     CDS             196..579
                     /gene="crcB"
                     /locus_tag="OO596_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6720016.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(633..842)
                     /gene="cspE"
                     /locus_tag="OO596_004387"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(633..842)
                     /gene="cspE"
                     /locus_tag="OO596_004387"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6720017.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(1017..>1311)
                     /gene="pagP"
                     /locus_tag="OO596_004388"
     CDS             complement(1017..>1311)
                     /gene="pagP"
                     /locus_tag="OO596_004388"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6720018.1"
                     /translation="NWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFT
                     AGVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          341 a          308 c          295 g          367 t
ORIGIN      
        1 gcgatacgtc gttttccgcc gcctgcgcca tcaacgaggc acaaatctca gcgttctttt
       61 cccacacaga tgtaacagca aactgtcctg cggcaaccag cattgccacc tcctgtcaga
      121 gtcgtaaaat tcgccaacga cgattattcg gcgttacact tatcactcat acaaatcaaa
      181 tagcaggatt ttgcagtgtt acaacttctt ttagcagttt ttattggcgg tggtacggga
      241 agcgtggcga gatggctgtt aagtatgcga tttaacccgc tgcatcaggc gattccgttg
      301 gggacgctgg cagcaaacct gattggggca ttcatcatag gaatgggatt cgcctggttc
      361 agcaggatga cgaacattga tccagtgtgg aaagtattaa tcaccaccgg attttgtggc
      421 ggtctaacaa ccttctcaac attttcggca gaagtggtgt ttttgttaca agagggccgc
      481 tttggctggg cgttactgaa cgttttcgtc aaccttctgg ggtcttttgc catgaccgca
      541 ctggctttct ggctgttttc ggcctcaacc gcacactaaa gaccaaaaaa aacccgctga
      601 ttaagcgggt tttgaattct tgctgacgta tcttacagag cgattacgtt tgcagcagaa
      661 gggcctttgg caccgttagt gatttcgaac tctacgcgct gaccttcagc aagagtttta
      721 aaaccattag tctgtattgc agagaagtgt acgaacacgt ctttgctgcc gtcttccgga
      781 gtaatgaaac cgaatccttt ggactcatta aaccacttaa cgttaccttt aatcttagac
      841 atcaaaatta cctttacatg aaaaatagac acaaatgctg tgtcggttac cagtacacca
      901 attgtggtac gctttgtcca gtgcaacttt actaaaaagt gataaaagtc gcgttatttt
      961 tgatgttact tattttagtt attgatttta aagaagttac taaaacttca tttgtctcaa
     1021 aactgaaagc gcatccaggc aaagtacaca ttgccattgt tgtaggtacc cggaatgtag
     1081 gtcatctgaa aagtcactgg gccataaccc acggaggcca atggcagtag aaccgggaga
     1141 gggatgtaat tccagttatc gcgtgccgtt acgccagcgg tgaatcccag acctaaatga
     1201 aaattttcat ccgccagcgg tcgccaggta ctttcccatc cgtatccggc aatcggttcc
     1261 catttgttcc acgagtcctt aaatgccatg gcatacaggc catgccagtt t
//
LOCUS       DAJTYZ010000331         1294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000331 DAJTYZ010000000
VERSION     DAJTYZ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.336"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1112
                     /locus_tag="OO596_004389"
                     /pseudo
     CDS             1..1112
                     /locus_tag="OO596_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAJTYZ010000332         1267 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000332 DAJTYZ010000000
VERSION     DAJTYZ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.337"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1116
                     /locus_tag="OO596_004390"
     CDS             <1..1116
                     /locus_tag="OO596_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6720019.1"
                     /translation="LRDIEVEAVTKMLACGTRILGVKEYNCDKPECPHVRYVTNSCGS
                     RACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDVCRLAVE
                     NLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGCLNKHGQWKKLSFLKDAMR
                     SRWMWNMRQRLLKAWSEGLAMPESLSHITTESQWRSLVLKAGGKYWHVYMSKKTAGGR
                     NTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTGETATETLTQRELVARLKQH
                     IPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFE
                     CVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          308 a          302 c          415 g          242 t
ORIGIN      
        1 ctgcgcgata tcgaggttga ggctgtcaca aaaatgctgg cctgcggcac gcggatactg
       61 ggagtgaagg aatacaactg cgataaacct gagtgccccc acgtcagata cgtcactaac
      121 tcatgcggca gccgtgcctg cccgtcctgc ggaaaaaagg ccacagacct gtggatagcg
      181 acacagctga atcgtcttcc tgactgcgac tgggtacatc tggtcttcac cctgccggac
      241 acgctgtggc cggtgttcga aagcaaccgg tggctgctga atgacgtgtg ccgtctggcg
      301 gtggagaatc tgctgtatgc cgcccggaaa cgggggcagg aacccggtat cttctgcgcc
      361 atccacacgt atggccgtcg tctcaactgg catccgcatg tacatgtgtc tgtaacctgt
      421 ggatgtctga ataagcatgg tcagtggaaa aagctgagct tcctgaaaga cgcgatgcgt
      481 tcgcggtgga tgtggaatat gcggcaacgg cttctgaaag cgtggtcaga ggggctggcg
      541 atgccggagt cgttgtcaca tatcacgacg gaatcacagt ggagaagcct ggtgctgaaa
      601 gccggaggaa aatactggca tgtgtacatg tcgaaaaaaa cggccggcgg gaggaatacg
      661 gcgcgctacc tgggtcgtta tctgaagaag ccgccgatag cggcctcccg gctggcacat
      721 tacaacggag gggcgagcct gagcttccgt tacctggacc acaaaacggg agaaacggcg
      781 acggaaacgc tgacacagcg tgagctggtc gcgaggctga aacagcacat cccggagaag
      841 ttttttaaga tggtgaggta cttcgggttc cttgccaacc gggtgtgtgg agagaagctg
      901 ccgcaggtgt accgtgctct ggggatggat aaaccggaac cagtggcgaa agtgtgctat
      961 gcacaaatgg tgaaacagtt cctgagtcgt gacccgttcg aatgcgtgct gtgtggcggc
     1021 cggatggtat accgccgggc cattgcgggg ctgaatgtgg aggggctgaa gaaaaacgcg
     1081 cgggatatca gtctgctgag gtatatgccg gcctgacaca ggctaagtgt gcccgggaat
     1141 cggtgcaagt ataaaaaaca gcctgttgcg gtataagaat cgaccatgca gagagagaaa
     1201 acaccggagt ggtggggaaa aacagaaagg ctcacgtggc ataaggcgcg gacaggggta
     1261 tcgctta
//
LOCUS       DAJTYZ010000333         1265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000333 DAJTYZ010000000
VERSION     DAJTYZ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.338"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(23..>1265)
                     /locus_tag="OO596_004391"
     CDS             complement(23..>1265)
                     /locus_tag="OO596_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6720020.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYLTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          334 a          299 c          227 g          405 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 acactgtttt acggtcttct gtagaaatct tagcgggaag gtaacctaat acacttcgtg
      901 aagtcaggat gtgtttaacg cttaatgctg accagttgcc atccggtttc ttagtcttgg
      961 atattctgac cttacgctga ttaatggtca ggtaaccttg ttcgtttagc catttacaga
     1021 tagcaaacat cgacatacca gaaagacgct tgtcatagat gatattcacc agtgacgctt
     1081 tatcttcatc aacgatgtag tatgaaccat cttctgatag ctctaaccat ccgggggctt
     1141 gcttacccat aggcttctta agttctcttg caagctgtgt tttatggttc cagacctgag
     1201 ctatacgctt ttgcttaaca agagattctt ggtgtgctct ctcagctatg atgatgtgct
     1261 taatc
//
LOCUS       DAJTYZ010000334         1232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000334 DAJTYZ010000000
VERSION     DAJTYZ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.339"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="OO596_004392"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="OO596_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6720021.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1098..>1232)
                     /locus_tag="OO596_004393"
     CDS             complement(1098..>1232)
                     /locus_tag="OO596_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6720022.1"
                     /translation="NENIVLLGPSGVGKTHLAIAMGYEAFKIFYDISKISLELYHNIH
                     "
BASE COUNT          432 a          193 c          205 g          402 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ccagtaatac gatattttca tt
//
LOCUS       DAJTYZ010000335         1224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000335 DAJTYZ010000000
VERSION     DAJTYZ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.340"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..773)
                     /locus_tag="OO596_004394"
     CDS             complement(<1..773)
                     /locus_tag="OO596_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6720023.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVESGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPP"
     gene            complement(773..1138)
                     /locus_tag="OO596_004395"
     CDS             complement(773..1138)
                     /locus_tag="OO596_004395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720024.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGG
                     MYCPECGTVHWPDGVAPPF"
BASE COUNT          289 a          355 c          308 g          272 t
ORIGIN      
        1 ggtggctttt tcaggtaaga gccaaagtac accgccacat gggtggcatt atccatcacc
       61 cgggatacgt tgacattcca gccacggcgg taatgcgtgt ccaggaagcg attccattcc
      121 cgtttactgc ttccttctgc tgccagcgca tccggcatca ccaggtcagg gtatttccgt
      181 gacagcaacc gtgttatccg gtagcgccac atgctcatca ccttacgggc gtaaaaatga
      241 agatttttcc aggtgtggcc cgacgtcaca ccaccggcag ttgtcgataa atggatatgc
      301 ggatgccact gctggtcacg cccccatgtg tggatcaccg tgaatatccc cgactccaca
      361 tctgcctgat ggcagatttc cagtatcaca tccgctgcaa tgcggctcat ctctgtcagt
      421 aaccaccggt tgtggaacac cagggaccag tactggcagg gaagtgtgaa cacaatatgc
      481 tgccacgggc agtcggggac caggctcagc agatactgta tccactgtgc gccagccttc
      541 accccgcagt gcgggcagga gcggctttta caccggaagc agaccttttt tgtatggcaa
      601 cagtccggtg atgaacagca ccactgtgta taccccatca gtgtggtccc gcacgccatg
      661 attttggtca ccgactcaat caccaccgga cgtactgccc cttccggctg cttctccagc
      721 cagttaagcc agcggtttcc ctgctgaaag atatcggcaa aacggggaag catcagaagg
      781 gcggggcgac tccgtccggc cagtgaaccg tgccacactc cgggcagtac ataccgccgg
      841 cgctgatacc ggaaagaatg gtcgcaaatt cccgctccgt gcagcgggcg atttccggat
      901 acccttcgtc atcaacacgt acaaaccaga agaccagctt tttgtttccc gcatccacaa
      961 agaacggaat attcaggtct gcgcagcatt caacggcatc gtcaaaacta tcaaagcgca
     1021 gaacttctgc gtcttcttcg tcaaaaaaat catcttcgtg aagcttcacg acatagcggg
     1081 gaagtttgct tctttgagag gcgggtttac gtttacgggg tttagctgaa cgggccatat
     1141 aaccacctga aagacaatga cattgcctgt ttttataacg gtaattgcag accatgacaa
     1201 gccgcagccg tcaggctgcc tact
//
LOCUS       DAJTYZ010000336         1213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000336 DAJTYZ010000000
VERSION     DAJTYZ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.341"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OO596_004396"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OO596_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6720025.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNV"
BASE COUNT          343 a          218 c          263 g          389 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagtctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtcg
     1201 tgagccgttt tca
//
LOCUS       DAJTYZ010000337         1212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000337 DAJTYZ010000000
VERSION     DAJTYZ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.342"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            229..>1212
                     /gene="gspA"
                     /locus_tag="OO596_004397"
     CDS             229..>1212
                     /gene="gspA"
                     /locus_tag="OO596_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCR6720026.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGN"
BASE COUNT          328 a          249 c          309 g          326 t
ORIGIN      
        1 catctttatt atgattcgcc agatggaagg ggaaacgtag tgtgggcacg atgtatgttc
       61 taataaaata gattgggtgg gttgtatatt gacgttagta taaatagtat taatgccgct
      121 tgcatgaatc aagcaaaatt tatttatatt agtaatatgt atttaattaa taaatacata
      181 cattattttt atgattaagg ataatcaata attaaaacag gaagttctat gtctacgaga
      241 agagaagtta ttctttcctg gttgcgtgag aaacgtcaga cctggcgtct gtgctatttg
      301 ttgggtgagg ctggaagtgg aaaaacctgg ctggcgcagc aactgcaaaa agataaacat
      361 cgccgtgtga ttactttaag cctcgttgtt tcctggcaag gtaaggccgc atggatcgtt
      421 accgacgata acgcggctga acagggctgc cgtgacagcg cctggacgcg agatgagatg
      481 gcggggcaat tactgcatgc gcttcatcga actgatagtc gctgccctct tattattatt
      541 gaaaatgctc acctgaatca ccgccgcata cttgatgatc tccaacgcgc gatatctctt
      601 attcctgacg gtcaattttt gctgatcggc agacccgatc gaaaagtcga acgtgatttt
      661 aaaaaacagg acattgaact tgtctcgaca ggacgtctga cggagcacga acttaaggca
      721 agcatccttg aaggtcagaa tattgaccaa cccgatctcc ttttaacctc cagagttctg
      781 aaacggatag ctttattatg tcggggcgat cgcagaaagc tggcgcttgc cggcgaaaca
      841 attcgcttat tacagcaagc cgagcagacc agcgttttca ctgcaaaaca atggcgaatg
      901 atttaccgca tattgggtga taaccgcccc cggaaaatgc agcttgccgt ggtaatgagt
      961 ggaaccatta tcgctattac ctgtggttgg ctactgctgt cctctttcac agctccgttc
     1021 cccgttcctg cgtggctggt acccgtaacg ccagtagtta aacaagatat gactaaggat
     1081 attgctcatg tggtgatgcg cgatagtgag gcgctaagcg tgttatacgg cgtatggggg
     1141 tacgaggtcc cagctgattc ggcctggtgc gatcaggcag taagagcggg gctggcatgt
     1201 aaaagtggga at
//
LOCUS       DAJTYZ010000338         1192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000338 DAJTYZ010000000
VERSION     DAJTYZ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.343"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(763..1192)
                     /locus_tag="OO596_004398"
                     /pseudo
     CDS             complement(763..1192)
                     /locus_tag="OO596_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          302 a          285 c          281 g          324 t
ORIGIN      
        1 ttattattta tcggtaacat gatggacatt cagttaaagc cgatggacaa atggctttgt
       61 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      121 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      181 gtgaatggcc tgtttgcacc gtatgtgaca gtaatacgct gcttcatgat aagccctcaa
      241 aaacatactt atgatatgta tatgagtata tattaatggt acaaaccatc aatatccata
      301 aaaaatacac cattaattcg ctataagttt ttaatacaat tttttctgct gcgatttttt
      361 tatacgataa ttagcatact tataacaccg taagtaaatt acatgaaagc aggcacagcg
      421 tgatccgatt tttctgcgta taacgatttt ccggaagtgt aatacggttg cgccccgggc
      481 cgggccccct tttctgttgt tgtgtacggg cccacagaga atggtaccgg aggcgggctc
      541 agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta ctgacacggg tattggtgac
      601 tggtgatgaa gtactgcccc gggtatgatt tcaggctgat tcaggttctg attctctgtc
      661 agtccgcgtg gttgctggcc tggctgattt cctgttgtca ggcgttcaac aaccgactgc
      721 tggttatagc ccggttcacg aacacctgac ggcaggtatc actcacccgg tgaaggtaaa
      781 tccctcaaga ggcagcaact ctgccagtcg ctcctccggc cattccggca gacgcgtcag
      841 gacgtccgtc agccaggcat gtggctccag accattacgt tttgctgttt acagaaggct
      901 cattatctgt gccgcccggt ttccggccat cagtgaaccg gcgaacagcc acgctttccg
      961 tcccataaca accgggcgaa tggcacgctc gcagatattg ttgtccaggg gcaccgctcc
     1021 gtcacccagg aagcagctca gttcatcgcg ccggttcaga atatagttaa tcgccttatt
     1081 taatggcccg tcgggcggac agcacggctc ctgctcttca agccaggcaa acaggtcatt
     1141 cactatcggc cgggagtacc gttatcgcca ctgccgtatt ttttccacag gg
//
LOCUS       DAJTYZ010000339         1190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000339 DAJTYZ010000000
VERSION     DAJTYZ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.344"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..295)
                     /locus_tag="OO596_004399"
     CDS             complement(<1..295)
                     /locus_tag="OO596_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6720027.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEP"
     gene            complement(343..915)
                     /locus_tag="OO596_004400"
     CDS             complement(343..915)
                     /locus_tag="OO596_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR6720028.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          380 a          246 c          220 g          344 t
ORIGIN      
        1 aaggttctaa tcgtgatact ggcggaataa cggtaaacgg cgctttgctt ttatcctctt
       61 tattaaatgg atctacccaa gcctggatga gataaggtat tttttcttct ttgctaaata
      121 cagaaataga tttatcagca ttattactta agtaaaccac ccgcgtacca ccaatgacaa
      181 cgcctgcata agttaatgct ggttggccta aaaaaacgct gagtataatc gcggtaaata
      241 acttacatga aactgttgtt ttaaagagcc tgattaaaca actttttctg gacataacga
      301 ctccctggcc aaagccaact gaataaaaag gcccttgata aattatttct tatattcgac
      361 agtaaattgt gaatcagcgt tcgccgtacc agccgttacg gtcgcattgg ttgccacata
      421 gcgtgcaata aacttcattt cagccttgcc ttcagcatcg atagcggtat aagcagagtc
      481 gttataaagt ttaacctgcg aattatcgga ctggttaaaa atacgaatac caacgccggt
      541 tgccgcaatc gcttgtcctg taccggtaaa atcaccaggg ttgcctgctg taagcggcgt
      601 acccactttc aggtcgccgt cgccgccagg cgcttctgta ccatcaaaac gaacggctgc
      661 ttgagtgtac gttgccgggc aattttcaag tttaatactg aacttctgtg gcgacgccag
      721 actacctaca cctgaaaatg catttttact gactttaccc agtgggacaa tttgatcggc
      781 tgtatcggag gtaacttcac aagtagtgtc gacaatttca ccgacgaagt gaacaacacc
      841 atcttctgcc agtgcatgcc cggccagcat tagagatgcc agagtgaatg caccgcctat
      901 aatattacgc ttcataaaaa ctccattgat ataatatatc ctttaccatg aatcatcact
      961 tatattaata agtgtatttt tgattcaaag gaatagtaca ccaaagagac ggctttatct
     1021 aaaaataata ttaattacgc aaaaataaaa tataaattca ttgtttaaat attatttaaa
     1081 cagtaaaaaa tcaagtttgt tatcagacat taatgataat tcagaatata tttatataga
     1141 aagatagata aatttattaa tgtcagttat aaaaaagggc caccggtcgg
//
LOCUS       DAJTYZ010000340         1148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000340 DAJTYZ010000000
VERSION     DAJTYZ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.345"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..187
                     /locus_tag="OO596_004401"
     CDS             <1..187
                     /locus_tag="OO596_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6720029.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="OO596_004402"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="OO596_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6720030.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          276 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgcttt
//
LOCUS       DAJTYZ010000341         1096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000341 DAJTYZ010000000
VERSION     DAJTYZ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.346"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1074
                     /locus_tag="OO596_004403"
     CDS             join(<1..167,167..1074)
                     /locus_tag="OO596_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HCR6720031.1"
                     /translation="EGASRLSLPEGTLGQWVTAARKGLGTPGSRTVAELESEILQLRK
                     ALNEARLERDIFKKSNSVFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQ
                     HEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRL
                     RCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIK
                     DVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQE
                     QSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQ
                     RRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          312 a          261 c          286 g          237 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtctggtctg
      841 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
      901 ggaacgctga aaaatgagag cctgagccac tatcgtttta ataaccggga tgaagccatc
      961 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
     1021 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag
     1081 aacaaatggt agtgtc
//
LOCUS       DAJTYZ010000342         1067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000342 DAJTYZ010000000
VERSION     DAJTYZ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.347"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            212..>1067
                     /gene="nadA"
                     /locus_tag="OO596_004404"
     CDS             212..>1067
                     /gene="nadA"
                     /locus_tag="OO596_004404"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6720032.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTA"
BASE COUNT          290 a          253 c          257 g          267 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcag
//
LOCUS       DAJTYZ010000343         1066 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000343 DAJTYZ010000000
VERSION     DAJTYZ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.348"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..580
                     /locus_tag="OO596_004405"
                     /pseudo
     CDS             1..580
                     /locus_tag="OO596_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          387 a          205 c          185 g          289 t
ORIGIN      
        1 aacgccacaa tctcctggga ttatttacta aaaataacgc ctgttttaat aagcattagc
       61 gatatgtatt ctgaagaaaa aatcaagttt gtcgaaagtt gtttaaatga gcttggagac
      121 attaccgaag aacaattaaa aattatgaga tttgcaatta taaaatctat accaagggca
      181 actcttacag ataaattaga aaatgaatta acaaaagaaa tatataaaag ctcatcgaaa
      241 atcaacaatt gcttgaacag aattaaatta ccagagatga aagaattctc atcagaaaaa
      301 aatatatgat tacatcgata taatcattga agattatgaa aatatcaaag aaaatgctta
      361 tctggtcgat ccaaaaatta attacactat agatttaaac atagaagact ctagctcaga
      421 ggaattcctt tttgataata gcctcgagaa agacgaaaat tcttcggaca attgctttga
      481 ggtcgaaaaa tataacgcat atgaagcata taactcagag aatctatatt ttaccagaga
      541 ggaatatacg tatgattacg accttttaaa tgcaatatag acataataac ttcttacaaa
      601 atctataatt aataccgtag atattcattc atcttaccct gtgcaccaaa agcaaaaact
      661 cactgacgcg ggtttgaaac tgatggcaac cgcaaaaatg cctgatgcgc tacgcttatc
      721 aggcctacgc catctctaca atatattgaa tttgcgtgct tttgtaggca ggataaggcg
      781 ttcacgcagc atccggcatg aataaagcgc actcaacaat ctgaaacccg ccggaacggt
      841 ttattacgta catcaggtga aactgaccga taagccgctt tcttttgggt atagtgtcgt
      901 ggacagtcat tcatctttct gcccctccaa aagcaaaaac ccgccgaagc gggtttttac
      961 gtaaatcagg tgaaactgac cgataagccg ctttcttttg ggtatagcgt cgtggacagt
     1021 cattcatctt tctgcccctc caaaagcaaa aacccgccga agcggt
//
LOCUS       DAJTYZ010000344         1056 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000344 DAJTYZ010000000
VERSION     DAJTYZ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.349"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            161..580
                     /locus_tag="OO596_004406"
     CDS             161..580
                     /locus_tag="OO596_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6720033.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            580..>1056
                     /gene="umuC"
                     /locus_tag="OO596_004407"
     CDS             580..>1056
                     /gene="umuC"
                     /locus_tag="OO596_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6720034.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
BASE COUNT          265 a          226 c          281 g          284 t
ORIGIN      
        1 ggatgagcgt cgtcgccaga aggccacgtg agcacaggat aagagaacga aaaatcagca
       61 gtctatgcag cgacaaatat tgatagcctg aatcagtatt gatctgctgg caagaacaga
      121 ttactgtata taaaaacagt ataacttcag gcagattatt atgttgttta tcaagcctgc
      181 ggatctccgc gaaattgtga cttttccgct atttagcgat cttgttcagt gtggctttcc
      241 ttcaccggca gcagattacg tcgaacagcg catcgatctg aatcaactgt tgatccagca
      301 tcccagcgcg acttacttcg tcaaagcaag tggtgattct atgattgatg gtggaattag
      361 tgacggtgat ttactgattg tcgatagcgc tattaccgcc agccatggtg atattgtcat
      421 cgctgctgtt gacggcgagt ttacggtgaa aaaattgcaa ctacgcccga cggtacagct
      481 tattcccatg aacagtgcgt actcgcccat taccatcagt agcgaagata cgctggatgt
      541 ctttggtgtg gtgatccacg tcgttaaggc gatgcgctga tgtttgctct ctgtgatgta
      601 aacgcgtttt atgccagctg tgagacggtg tttcgccctg atttatgggg taaaccggtg
      661 gttgtgctat cgaataatga cggttgcgtt atcgcccgaa acgctgaggc aaaggcgctg
      721 ggtgttaaaa tgggcgatcc ctggttcaaa caaaaagatc tgtttcgtcg ctgtggtgtg
      781 gtttgcttta gcagcaatta tgagctttac gcagacatga gcaatcgggt gatgtcgacg
      841 ctggaagaac tatcgccccg cgtcgagatt tacagtattg atgaggcatt ctgcgatctg
      901 acaggtgtgc gtaattgtcg cgatctgact gattttggca gagaaattcg cgcaacggtg
      961 ctacaacgta cccatcttac tgttggtatg gggatcgccc agaccaaaac gctggctaag
     1021 cttgccaatc atgcggcaaa aaaatggcag cggcag
//
LOCUS       DAJTYZ010000345         1054 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000345 DAJTYZ010000000
VERSION     DAJTYZ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.350"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(59..262)
                     /gene="tatE"
                     /locus_tag="OO596_004408"
     CDS             complement(59..262)
                     /gene="tatE"
                     /locus_tag="OO596_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6720035.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(391..>1054)
                     /locus_tag="OO596_004409"
     CDS             complement(391..>1054)
                     /locus_tag="OO596_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6720036.1"
                     /translation="LARDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVP
                     STPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGL
                     MTCYDLRFPELALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAG
                     ECGNKNIGQSRIIDPFGVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQ
                     LL"
BASE COUNT          264 a          303 c          243 g          244 t
ORIGIN      
        1 ttttgaagcg gtttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc
       61 actctttatg agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag
      121 catcgtcatc attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca
      181 gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt
      241 tggtaatact aatctccccc atagatacct tcttgacata taaccggcaa cagaacgcgc
      301 cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac
      361 aaggtgaatc aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg
      421 gttgtttaag acgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc
      481 cataatgagt gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat
      541 ccggctttgc ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt
      601 atccagcgca cgagcggcaa gcaacgttga ccagtgatgc tctttgagcg gcccgcgaac
      661 ccaggcggca ggaagtacca ggatttcagc tccctgtaat gcctgtgcca gcgccagctc
      721 tggaaagcgt aagtcataac aggtcatcag accgaccttc atcccctcca cctcgagtaa
      781 cggagcgatt tcattaccag catcaacacg gcgtgattcc tgaatggcaa atgcatcata
      841 gagatgcaat ttggcataac gggcgacgat gtttcctgcc tgtagcgcca ccagcatatt
      901 ccatgcgcgc cccggcgttg aaggaacatg aatcgtcaga attgtcgtca tcatgttacg
      961 tttactttct cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac
     1021 cgatagatct gcatcatgat catcgcgcgc cagc
//
LOCUS       DAJTYZ010000346         1047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000346 DAJTYZ010000000
VERSION     DAJTYZ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.351"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO596_004410"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO596_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6720037.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
BASE COUNT          339 a          154 c          183 g          371 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatctacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcagaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaaaga gagctaaata gctgcgc
//
LOCUS       DAJTYZ010000347         1033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000347 DAJTYZ010000000
VERSION     DAJTYZ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.352"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     misc_feature    6..122
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(173..397)
                     /locus_tag="OO596_004411"
                     /pseudo
     CDS             complement(173..397)
                     /locus_tag="OO596_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(586..>1033)
                     /locus_tag="OO596_004412"
     CDS             complement(586..>1033)
                     /locus_tag="OO596_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6720038.1"
                     /translation="VYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTKREEYLLMS
                     GDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAMQITWRGMY
                     QRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHELLARMRWCG"
BASE COUNT          242 a          307 c          215 g          269 t
ORIGIN      
        1 gcttccgctt attttgcgaa ggcggagttc gaccgcctct ggaaaaaatg atgccactgc
       61 tggataagct gcgtgagcag tacggggtcg gaccgctatg cagcgaactg catattgccc
      121 cgtcaacgta ttagggattt gaagcccaac cgtacgaaaa cgtacgctaa gttcatttct
      181 tgaacaacct ggctgactct atgtatttgt acagcgttgg cctcgatatc cccatcaaca
      241 cacaaatctg cgcaactgta tgttttttct cgttatagag ttgaacagca agggcctgtt
      301 tatccttact cagtgttttc ggcctgccgc ccttacgtcc tctggctcgt gctgcttgaa
      361 gcccgacctg agttctctct cttgtcaggt tgcgttcatc gataggaatt aaaaccccaa
      421 aaagattaaa aaaacaccac aaaacggatg tttcttcaac accacttttg ctccatatga
      481 acggaaccga cgattaaact ggatggctct gattgattca gggtatgaat ggcggttttt
      541 tgctccgttt ccctcaaaat ggacgcaact tcccctctgc ggctctcagc cgcaccaccg
      601 catccgggcc agcagctcat gcatcaggac ctgctctgcc agacggtagc cccgcttcag
      661 ccccgtaaaa cgcatctgac tcccgcacag cacgcacttc agcgggtcaa ccttcagtaa
      721 cctctgatac atccctctcc aggtgatttg catcgccgtt tttctcactg tctccgttat
      781 gatgtacacc acttcttcca gtaaccgccg tttcgccgga ctcaaaaaac cgtagtacct
      841 caccatacgg aaccccttat ccgccacatg ccaggagaac ctttccatga actcatctcc
      901 actcatcaac aggtattctt cccgttttgt tcggtgactg ttgtaacgca gaccgatttc
      961 atcctgaccg gcataatgct ccagacgact catcggcacc ggtggctttt tcaggtaaga
     1021 gccaaagtac acc
//
LOCUS       DAJTYZ010000348         1028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000348 DAJTYZ010000000
VERSION     DAJTYZ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.353"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            66..>1028
                     /gene="istA"
                     /locus_tag="OO596_004413"
     CDS             66..>1028
                     /gene="istA"
                     /locus_tag="OO596_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR6720039.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFD"
BASE COUNT          246 a          275 c          271 g          236 t
ORIGIN      
        1 gatccacacc caacgcctga aatcagatcc agggggtaat ctgctctcct gattcaggag
       61 agcttatggt cacttttgag acagttatgg aaattaaaat cctgcacaag cagggaatga
      121 gtagccgggc gattgccaga gaactggggc tctcccgcaa taccgttaaa cgttatttgc
      181 aggcaaaatc tgagccgcca aaatatacgc cgcgacctgc tgttgcttca ctcctggatg
      241 aataccggga ttatattcgt caacgcatcg ccgatgctca tccttacaaa atcccggcaa
      301 cggtaatcgc tcgcgagatc agagaccagg gatatcgtgg cggaatgacc attctcaggg
      361 cattcattcg ttctctctcg gttcctcagg agcaggagcc tgccgttcgg ttcgaaactg
      421 aacccggacg acagatgcag gttgactggg gcactatgcg taatggtcgc tcaccgcttc
      481 acgtgttcgt tgctgttccc ggatacagcc gaatgttgta catcgaattc actgacaata
      541 tgcgttatga cacgctggag acctgccatc gtaatgcgtt ccgcttcttt ggtggtgtgc
      601 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
      661 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      721 cccgactgtg tcgccccttc agggcacaga ctaaaggtaa ggtggaacgg atggtgcagt
      781 acacccgtaa cagtttttac atcccactaa tgactcgcct gcgcccgatg gggagcactg
      841 tcgatgttga aacagccaac cgccacggtc tgcgctggct gcacgatgtc gctaaccaac
      901 gaaagcatga aacaatccag gcccgtccct gtgatcgctg gctcgaagag cagcagtcca
      961 tgctggcact gcctccggag aaaaaagagt atgacgtgca tcctggtgaa aatctggtga
     1021 gcttcgac
//
LOCUS       DAJTYZ010000349         1023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000349 DAJTYZ010000000
VERSION     DAJTYZ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.354"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(77..865)
                     /locus_tag="OO596_004414"
     CDS             complement(77..865)
                     /locus_tag="OO596_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6720040.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="OO596_004415"
     CDS             complement(905..>1023)
                     /locus_tag="OO596_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6720041.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAJTYZ010000350         1001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000350 DAJTYZ010000000
VERSION     DAJTYZ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.355"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1001
                     /locus_tag="OO596_004416"
                     /pseudo
     CDS             1..1001
                     /locus_tag="OO596_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          244 a          244 c          306 g          207 t
ORIGIN      
        1 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
       61 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      121 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      181 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg
      241 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      301 gagagctgcc cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac
      361 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gatcatgcgc cagaatgcgc
      421 tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      481 ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc tgtgataacg
      541 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca ctgcactggg
      601 cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      661 aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg gataatggtt
      721 catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt gaaccgaaga
      781 acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg aaaacgataa
      841 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      901 cagaggcgtt cgagcattat aacgaatggc atccgcatag tgcgctgggt tatcgctcgc
      961 cacgggaata tctgcggcag cgggcttgta atgggttaag t
//
LOCUS       DAJTYZ010000351          997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000351 DAJTYZ010000000
VERSION     DAJTYZ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.356"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..937
                     /locus_tag="OO596_004417"
     CDS             <1..937
                     /locus_tag="OO596_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HCR6720042.1"
                     /translation="SQQIYFVPFMTDGSGVNTPTNNPSEDPDIAGSGYYGSASRTNKN
                     WVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKAPEIKPSPGGDTPSGPS
                     EDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQSNKAWSLTRPVDDAVSL
                     LTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTGTGNPFLMSFFTQTTDG
                     KLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPKLNGVAGPAFQVIKDSL
                     TLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
BASE COUNT          252 a          253 c          274 g          218 t
ORIGIN      
        1 atcccagcag atttattttg ttccctttat gaccgatggt agcggagtta atacaccgac
       61 aaacaacccg tcagaagatc ctgatattgc cgggtctggt tattacggtt cggcatcccg
      121 aacgaacaaa aactgggtat catcaaatcg cccgacgcat ttcagctcat gggcgcgtcg
      181 tggcattatt cccgatcgta tggcaactgc tattctgaac gtagccggtc gcaccttagc
      241 cttcattagt ggtaaggcac cggaaatcaa accctcgccc ggcggcgaca cgccatcggg
      301 gccgtctgaa gatgcatcca tacgcacaat ctccctgttg ccgacagccg gagacgctgc
      361 tgcgcagggc tggagcatta agaatggcgg agttcagttg tcagatggtg tatttaagat
      421 caccaagcag agcaataaag cctggtccct gacgcgcccg gtggatgacg cagtctccct
      481 gctgacacgg ggtggcagac tgagctgtaa gtttcgactg tcaggcgcac tgaccaacaa
      541 ccagttcggt ctgggaattt atctgtatac cgatgtagcg ttacctgacg tcgtggcgat
      601 gaccgggact ggtaacccgt tcctgatgtc gttcttcacc cagaccacag acggcaaact
      661 gaatctgatg catcacaaga aagcaggaaa cacaaagttg ggcgagttcg ggaattacag
      721 taacgactgg ctgacgctgg agctggtgtt caccgccggc agtgccacgg ttactccgaa
      781 actgaatgga gtggctggcc cggcattcca ggtcataaaa gacagtctga cactggggct
      841 gaatgcgctg acgctgacgg atattaccaa aaatgcagcg tatggcgttg agatagaaag
      901 tctggtgctg gagataaatg caccggcatc atcataaaaa gtgagccagc caaatggaag
      961 gtatcgttaa actcaccggt agtgtcagtg ggtcgtc
//
LOCUS       DAJTYZ010000352          996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000352 DAJTYZ010000000
VERSION     DAJTYZ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.357"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..389
                     /locus_tag="OO596_004418"
     CDS             <1..389
                     /locus_tag="OO596_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6720043.1"
                     /translation="RERELLSGFVDYLCESVGREHTPALSEAAEQYLNTVATRRTRDT
                     ALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDETEFQQVYKSVLNVL
                     WNWILFRKFSSPEQVENVAAQLLEFA"
     gene            389..679
                     /locus_tag="OO596_004419"
     CDS             389..679
                     /locus_tag="OO596_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6720044.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            676..>996
                     /locus_tag="OO596_004420"
     CDS             676..>996
                     /locus_tag="OO596_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6720045.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRV"
BASE COUNT          268 a          209 c          286 g          233 t
ORIGIN      
        1 ctcgcgaacg agaactgctg tctggtttcg ttgattacct gtgcgaatca gtaggtcggg
       61 aacatacgcc agctctgagc gaagccgcag agcaatatct gaatacagtt gcgacacgca
      121 gaacccggga tacggcattg ctaaagtcgt ttgaggcttt tcgcgagtgg gtaaccattc
      181 aggctggatt ttacaccgaa catttttatc cggacggtag ccgcgggcgt cgggcaaaat
      241 ccatcgcttt tgcgaatatg gacgaaaccg agtttcagca ggtttataaa tctgttctga
      301 atgtgctgtg gaactggatt ctgttccgta aattttcctc tccggaacaa gtcgaaaatg
      361 tggccgcgca gctgctggag tttgcgtaat ggtggattta cgtaaagcgg cgcgggggca
      421 gatgtgcacc gtcagaattc ctggctactg caatcacgat ccggaaacgt ctgtgctggc
      481 gcattaccga ctggcgggaa cgtgcggaac agcgataaag ccacacgata tgcaggcagc
      541 gattgcctgt agctcgtgcc acgatttaat cgacgggcgg gtaaaaacca gcgattacac
      601 caaagaagaa ttacgcctga tgcatgcaga aggtgttttt cgcacacaag aaatctggag
      661 aaaggaggga tatttatgat ttacccaacg aatacaggaa aaagcggaga acaccttcgt
      721 ctcaccacgc tggaaagtgt ctggattcag ggaaaactac gtatgtgggg gcgctggtcg
      781 tatattggtg gcggtaagac ggggaatatg tttaacctga tgttgacctc taaaaagctg
      841 acaaaaacgg caattaacga ggcgctccgg aggatgaaaa aagcaggtct gaacaagtct
      901 gaacttgagg cttttttgcg ggatatgatt aacggtaagc aaaagagctg gctggcgcat
      961 tgtactgatg cagaggcgtt atgtattgat cgggtc
//
LOCUS       DAJTYZ010000353          984 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000353 DAJTYZ010000000
VERSION     DAJTYZ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.358"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(50..193)
                     /locus_tag="OO596_004421"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="OO596_004421"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..>984)
                     /locus_tag="OO596_004422"
     CDS             complement(197..>984)
                     /locus_tag="OO596_004422"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6720046.1"
                     /translation="FSLQDSRGGSLSKKAESLSSGKITIGTKEKVIIINELRQCHPLS
                     QLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGYRRIRLACRNESI
                     LLNGKTIRKLMKELGISSLIRRKKYRTYRGEQGRTCNNLLKRQFYADRPNQKWVTDVT
                     EFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQLNKDDKPLLHSD
                     QGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
BASE COUNT          262 a          205 c          181 g          336 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaac tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aact
//
LOCUS       DAJTYZ010000354          958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000354 DAJTYZ010000000
VERSION     DAJTYZ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.359"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..958
                     /locus_tag="OO596_004423"
                     /pseudo
     CDS             6..958
                     /locus_tag="OO596_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          236 a          279 c          293 g          150 t
ORIGIN      
        1 cgtagggtga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
       61 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
      121 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
      181 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
      241 acgggctgga aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc
      301 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
      361 cggaagatgc caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga
      421 ccgtcactga ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga
      481 cggcggtgca ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt
      541 taattgcggt gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg
      601 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
      661 gaaaagccat ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
      721 gacgggtcat ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
      781 tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
      841 tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg
      901 aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggta
//
LOCUS       DAJTYZ010000355          955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000355 DAJTYZ010000000
VERSION     DAJTYZ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.360"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>955
                     /locus_tag="OO596_004424"
     rRNA            <1..>955
                     /locus_tag="OO596_004424"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          262 a          206 c          307 g          180 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg
      661 taatagctca ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accatgcacc
      721 gaagctgcgg cagcgacgct tatgcgttgt tgggtagggg agcgttctgt aagcctgcga
      781 aggtgtgctg tgaggcatgc tggaggtatc agaagtgcga atgctgacat aagtaacgat
      841 aaagcgggtg aaaagcccgc tcgccggaag accaagggtt cctgtccaac gttaatcggg
      901 gcagggtgag tcgaccccta aggcgaggcc gaaaggcgta gtcgatggga aacag
//
LOCUS       DAJTYZ010000356          950 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000356 DAJTYZ010000000
VERSION     DAJTYZ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.361"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(211..>950)
                     /locus_tag="OO596_004425"
     CDS             complement(211..>950)
                     /locus_tag="OO596_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6720047.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          284 a          204 c          201 g          261 t
ORIGIN      
        1 caggctacct cacattgctt ctccgataaa atgtcagaga agcatgatta cgtggttatt
       61 gtctgttgat gacttgttga ccgagccgtc atcattctca atgcattagt taaatcattt
      121 gtgattttta aggattatcc tgtctatgat tttctactta gaaggatatg gcactaacta
      181 tgcatcttat agtttactgc tggcatctat ttaaaatagc aattgagtca aatagcccat
      241 caagatagcc atacctaata taaccgtcag gaatgcagct atcatcggca ttctgaacat
      301 tgatttaagt aatatcactt ccgtcaggct tgcgccggca ctgccgatga ttaatgccat
      361 taatgctccc agacccattc cttttgtcat cagaacagat gccagaggga taaccgcctc
      421 agcccggata tacagcggaa taccgacaac ggcgcttaat gggatggcga agggattatc
      481 tgctcctgca tgagcggcaa tccacgctga gggaataaag ccataaataa aagatcctat
      541 taatactcct aacaaaagat atggcagtac atctttaaac tgttgcaacg catctttggt
      601 ggctagtttt atgggattaa tacttactac agctttggta ctgcaacagg taactacggg
      661 gttctcaatg gctttagccg ctggactaca acaactcact tttatgggcg tatatgtcgt
      721 tcccggcgaa gtttttgctg gagcacaaca atttgctgat gagcttttac tggcaatgat
      781 atgacgttca aaccccagag aatccaggat aatactggca agaacggaga cgccggctgc
      841 gataatcgcg tacaacaagg tgactttcca accaaaggtc atccacatta gtccgacgat
      901 aatgggatta agtaatggtg aaacaaataa aaaagtgaga gtcggaccaa
//
LOCUS       DAJTYZ010000357          947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000357 DAJTYZ010000000
VERSION     DAJTYZ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.362"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..441
                     /gene="csgB"
                     /locus_tag="OO596_004426"
     CDS             <1..441
                     /gene="csgB"
                     /locus_tag="OO596_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6720048.1"
                     /translation="LFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFNQAAII
                     GQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDASISQG
                     AYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            482..934
                     /gene="csgA"
                     /locus_tag="OO596_004427"
     CDS             482..934
                     /gene="csgA"
                     /locus_tag="OO596_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR6720049.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          262 a          195 c          239 g          251 t
ORIGIN      
        1 ttatttatga tgttaacaat actgggtgcg cctgggattg cagccgcagc aggttatgat
       61 ttagctaatt cagaatataa cttcgcggta aatgaattga gtaagtcttc atttaatcag
      121 gcagccataa ttggtcaagc tgggactaat aatagtactc agttacggca gggaggctca
      181 aaacttttgg cggttgttgc gcaagaaggt agtagcaacc gggcaaagat tgaccagaca
      241 ggagattata accttgcata tattgatcag gcgggcagtg ccaacgatgc cagtatttcg
      301 caaggtgctt atggtaatac tgcgatgatt atccagaaag gttctggtaa taaagcaaat
      361 attacacagt atggtactca aaaaacggca attgtagtgc agagacagtc gcaaatggct
      421 attcgcgtga cacaacgtta atttccattc gacttttaaa tcaatccgat gggggtttta
      481 catgaaactt ttaaaagtag cagcaattgc agcaatcgta ttctccggta gcgctctggc
      541 aggtgttgtt cctcagtacg gcggcggtaa ccacggtggt ggcggtaata atagcggccc
      601 aaattctgag ctgaacattt accagtacgg tggcggtaac tctgcacttg ttctgcaaac
      661 tgatgcccgt aactctgact tgactattac ccagcatggc ggcggtaatg gtgcagatgt
      721 tggtcagggc tcggatgaca gctcaatcga tctgacccaa cgtggcttcg gtaacagcgc
      781 tactcttgat cagtggaacg gcaaaaattc tgaaatgacg gttaaacagt ttggtggtgg
      841 caacggtgct gcagttgacc agactgcatc taactcctcc gtcaacgtga ctcaggttgg
      901 ctttggtaac aacgcgaccg ctcatcagta ctaatacatc atttgta
//
LOCUS       DAJTYZ010000358          938 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000358 DAJTYZ010000000
VERSION     DAJTYZ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.363"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            84..>938
                     /locus_tag="OO596_004428"
     CDS             84..>938
                     /locus_tag="OO596_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR6720050.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIY"
BASE COUNT          253 a          241 c          255 g          189 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttat
//
LOCUS       DAJTYZ010000359          914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000359 DAJTYZ010000000
VERSION     DAJTYZ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.364"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>914
                     /locus_tag="OO596_004429"
     CDS             <1..>914
                     /locus_tag="OO596_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6720051.1"
                     /translation="EGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMN
                     AWQTQDWVALGGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGK
                     GRAFCEKLAQQAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREIS
                     RSAAESTLMIAVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNI
                     AFAGASELVREVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDD
                     DKPRLGDFRRQLIGQVKETLQKGKTPSE"
BASE COUNT          194 a          218 c          298 g          204 t
ORIGIN      
        1 gaggggcaag ttgaagcggt aatggatgca gcgttacgtc cgaaacgcag cctgtggcgc
       61 aaaatggtga tgggcgggct ggctctgttt ggcgcaagtg ttgtcgggca gggtgtacag
      121 tggacaatga atgcctggca aacccaggac tgggtagcgc tgggtggatg tgccgctggg
      181 gcattgatta tcggcgctgg cgtaggttct gtggtaacag agtggcagtg cttatggtgc
      241 ttgcgacagc gcgcccatga acgcgacgaa gcgcgtgatt tattgcatag ccacggcacg
      301 ggcaaaggcc gcgcattttg cgaaaaactg gcgcagcagg cgggtattga tcagtcgcat
      361 ccggcgctgc aacgctggta tgcctcaatc catgaaacgc aaaacgaccg tgaagtggtc
      421 agcctgtatg cgcatttggt ccagccagtt ttagatgccc aggcgcggcg cgaaatcagc
      481 cgttcggcgg cggaatcaac gttgatgatt gcggtcagcc cgctggcgtt ggtcgatatg
      541 gcgtttatcg cctggcgcaa tctgcgttta attaatcgca tcgccacgct gtatggcatt
      601 gaactggggt attacagccg tttgcgtctg tttaagctgg tattgctgaa tatcgctttt
      661 gccggagcca gcgaactggt gcgcgaagtg gggatggact ggatgtcgca agatctcgct
      721 gctcgtttgt ctacccgcgc agctcagggg attggtgcag gacttctgac ggcacgactc
      781 gggattaaag ctatggagct ttgccgcccg ctgccgtgga ttgacgatga caaacctcgc
      841 ctcggggatt tccgtcgtca gcttatcggt caggtgaaag aaacactgca aaaaggcaaa
      901 acacccagcg aaaa
//
LOCUS       DAJTYZ010000360          881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000360 DAJTYZ010000000
VERSION     DAJTYZ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.365"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..833)
                     /locus_tag="OO596_004430"
     CDS             complement(<1..833)
                     /locus_tag="OO596_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6720052.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     M"
BASE COUNT          242 a          249 c          220 g          170 t
ORIGIN      
        1 ttcattttgc gcttaacttc agcgtagata atgaaaggca cgatcgagcc aaaggcgatt
       61 aacatcgtcg ccagatagac cttccagtgt tcagccgccg ggaaccccgc atcagctagc
      121 tgtccgggca gggcaacaaa cgtagacatc agaagcatgt gcagacacat aatgccaaag
      181 ttgagtttca gcagccgcgg ttccgccagc actttactga aactgccttt caccattccg
      241 gactcacgat taagtacgtg agtgctactg ttgggcacaa cccaaatggt caacgcaatg
      301 ccggttgttg ccagaatagc gatcatccag aacagcgcgt gcagcccaag tttgtgagtg
      361 atgatcgggc caagcaccat cgcaatggca aaggtaatgc caaagctcac gccgataaac
      421 gccatcgctt tggtgcggtt ttgttcgcgc gtgagatcgg aaagcagcgc cataacggcg
      481 gcggcaattg ctccagagcc ttgtagcgcc cggcccagaa taattcccca gatggagtcg
      541 gaaagcgcag cgataacgct accggcggca aacaccgcca gcccaccgac aattaatggt
      601 ttgcgaccaa tgcggtcgga aagcaggcca aacggaatct gaaaaacggc ctgagtcaga
      661 ccataaatac caatggcaat accgattaat gcttcgctgg caccttgcag agccatgccg
      721 tacgtggtca gaaccggcag aaccataaac atgcccagca tgcgcaacga gaatacggtc
      781 cctaaacccc aggtcgcgcg cctctcacct ggcgtcattt tataatcgtt cattaccacc
      841 ccttaaaaaa atgcccacat agtgtagtgc ggagcgcatg t
//
LOCUS       DAJTYZ010000361          873 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000361 DAJTYZ010000000
VERSION     DAJTYZ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.366"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..485)
                     /locus_tag="OO596_004431"
     CDS             complement(<1..485)
                     /locus_tag="OO596_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6720053.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     L"
     gene            complement(485..700)
                     /gene="essD"
                     /locus_tag="OO596_004432"
     CDS             complement(485..700)
                     /gene="essD"
                     /locus_tag="OO596_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCR6720054.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          230 a          224 c          193 g          226 t
ORIGIN      
        1 cacaaacaga cttcacgctc aatctcacgg cgagtcatca gtcctttcca ttgattaccg
       61 ccagcgtatg tccagcgccg tagctgatca catgcgcctt taatatcacc ctggtttatt
      121 ttgcgaagaa gcgtcgatgt tctgaaattg ccagcaccca cgttgtagac gaacgagtaa
      181 agagcgcagc gcgttgtttc cggtatatcg actttgatgt acgggttaat ttgtctggcg
      241 acagtggcaa ggtctttatt caggagggct ttgcactctg cttcggtata cgttttaccg
      301 ggaatgatgt cttttcctgt atgcccgtaa catacagtcc atacaccaac tatgtctttg
      361 taaggattat gtctcacacc ttccagacca tcgttaccac ctgggccagt gattaacaca
      421 gatgctatag caatagcccc gccaccaata gcagcagcaa cagcttttcg taatgatgga
      481 ggcattatcc acctctcgca gccttgcgct tatcttcttt aatcttgaaa taaaggtttg
      541 tcaggtacgt cagcaggcca aataccaggc tacccagcac tccaattgcc gcccactgtg
      601 agggcgtgac tttatctagc agctgtaaaa accagtaccc ggcactacct gctgaggtgc
      661 cataggcgac acccgttgtt aacttatcca tggatttcat aaccccacct cgcagacaaa
      721 gcgggtgtaa attgagggaa tactacgaaa cgtaacagac tcggagtcag tgaataactc
      781 aggtattggg ttatcagcta atatcgagac tcaaaaaatg gaaaaacccg ctcgacggcg
      841 ggtttaagct gtgtgacgaa gtaaccactc tta
//
LOCUS       DAJTYZ010000362          866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000362 DAJTYZ010000000
VERSION     DAJTYZ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.367"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(262..>866)
                     /locus_tag="OO596_004433"
     CDS             complement(262..>866)
                     /locus_tag="OO596_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6720055.1"
                     /translation="SEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHF
                     NLWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQ
                     RVSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARH
                     VSTHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
BASE COUNT          205 a          242 c          201 g          218 t
ORIGIN      
        1 tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg gcgtggaagt tgtcgatatt
       61 gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg acaataacca cgattaacac
      121 ccaatgcagc aggcagtttt tatctttgga cagcgaattc agaagtattt ttagcggact
      181 gcaactttta catttcagcg gaagctgact cgcaaattcc ccatgaaaat actccggcag
      241 cgttagccac cggagaggaa attatttcag cgatccctta aggaactgtt gcagacgagg
      301 gctttgcggg ttgccaaata actgctccgg cgcgccctct tcttctattt tcccctgatg
      361 gaggaaaatg acgtgagtag aaacatggcg agcaaagccc atttcgtgag tcactaccac
      421 catggttttc ccctcttctg ccagttgctg cataatacgt aacacttcgc ctaccagttc
      481 aggatcgagc gccgaggtag gttcatcaaa cagtaaaact tccggttcca tcgccagcgc
      541 ccgcgcgata gaaacacgct gttgctgacc gccggaaaga tgcaccggat atttcccctg
      601 cgcacgttcg tctatcccga ctttcgccag atacttcacc gcccgctcgc gcgcttcctg
      661 cttgctcagg cccaacacct gaatcggcgc ttccatgacg ttttccagca ccgtcatatg
      721 gctccagaga ttgaagtgct ggaataccat cgtcaggcgt gtgcgcagta agcgcagttg
      781 atttttatcg gcgactttga gttgaccgtc tttgtcgcgc accagattga tcgtctggcc
      841 attgaccacg atcgaccctt cactcg
//
LOCUS       DAJTYZ010000363          861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000363 DAJTYZ010000000
VERSION     DAJTYZ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.368"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..189
                     /locus_tag="OO596_004434"
     CDS             <1..189
                     /locus_tag="OO596_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1870 family protein"
                     /protein_id="HCR6720056.1"
                     /translation="QRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWK
                     IYQSVAAELYAHDLERLC"
     gene            289..>861
                     /locus_tag="OO596_004435"
     CDS             289..>861
                     /locus_tag="OO596_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6720057.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRL"
BASE COUNT          207 a          194 c          179 g          281 t
ORIGIN      
        1 caacgaaaaa aacatcttca tgccatcata gagaaaataa ataaccgcat cggcaacaac
       61 accatgcgtt ttttccccga cttaaccgcg tttcaacaag tctaccctga tggcaatttt
      121 attgactgga agatctatca atcggtagcc gctgaattat atgcacacga tctggagcgg
      181 ctttgttaat ttttccacag aaaggaattg tcgttgttac aacaataatg aatggatact
      241 gacacaacat cgctacactt tttaaagcac ctttgctaag tagaacctat gaaaaatccc
      301 tattacccta ccgcacttgg gttgtatttt aattacctgg tgcatggtat gggcgtcatt
      361 ttgatgagcc tgaatatggc ctcgctggag acactttggc agactaatgc cgcgggtgtc
      421 tcgatagtta tctcatcgct gggcattggt cgattaagtg tcttgctttt tgcaggatta
      481 ttatccgatc gctttggtcg ccgccctttt atcatgctcg ggatgtgctg ctatatggcc
      541 ttcttttttg gcatcctgca gaccaataac atcattatcg cttatgtttt tggctttctg
      601 gcgggaatgg caaacagttt tctcgatgca ggcacttatc ccagtttgat ggaagctttt
      661 ccacgctcac ctgggacagc caatatttta attaaagcat ttgtttccag cggacaattt
      721 ttattaccgc taatcattag cctgttagtg tgggctgaac tgtggttcgg ttggtccttt
      781 atgattgctg caggcattat gtttattaac gctctgtttt tataccgttg tacgttccca
      841 ccccatccgg gtcgtcgctt a
//
LOCUS       DAJTYZ010000364          860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000364 DAJTYZ010000000
VERSION     DAJTYZ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.369"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          286 a          165 c          131 g          278 t
ORIGIN      
        1 gcaagccctt ttcttgtgtt gtcaagacat ttggcaacgt tcaaacatct gtttagaaat
       61 gccaaattgt tgatctaaca aataaacctg tataaacaga acaccattta atctattccc
      121 ctgtgtaacc caccacatca cacaataaaa acaaaaaact ttaattatat ttcatagcac
      181 acaaaagtca atagaagccc tttcgtgctc ctacgtaaaa aatccatcac ttttccactg
      241 atacatcaat tcaaggcgtt atatgtgttc cttagcacat ataacattta ttatgttaaa
      301 tggattttgg cctatatttt gacctgcacc cgcataaaca ctggtttcat cgcttacagc
      361 ccttgtgaga cgtggtgctg tttctagcat ctgatccgac ataaaaccga tcattctcgt
      421 gtttagtgaa agtttagtga gagacattac agaaaggctt tctgtgtaat gatttaatag
      481 gtaaaaacga tgttgattac gcaagaaaat tccttgctga tatttgggga ttctggattt
      541 tccagatcgg ggggcgctga aaatcgcgtt aagcacctct cgcaatctag ccataaggga
      601 agggatatag gtattaagat gtatttatag ctatttatgg ctatttattt tttatttacc
      661 tttacttaaa aataacctta taaatcaata agatacataa ttatctgttt atattttaat
      721 caattaaatc aagtttaata cccttaaatc acctgaaaat agccttaaat accccaataa
      781 ttaattttaa ggttttccgt gttctcttcc gcattatgaa aatgcaaaat aaggcacaaa
      841 acaagcgcac agacgcatta
//
LOCUS       DAJTYZ010000365          859 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000365 DAJTYZ010000000
VERSION     DAJTYZ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.370"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>859
                     /locus_tag="OO596_004436"
     CDS             <1..>859
                     /locus_tag="OO596_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HCR6720058.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENF"
BASE COUNT          252 a          189 c          217 g          201 t
ORIGIN      
        1 agtggtaacc tgatcgaagg tctcactgtg tactttgcct taaaaagcgg ctctgccaca
       61 ttaacgtcat taacagcggt gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga
      121 gcgatgacag gtagcgtcac ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta
      181 gatataacgc tggtggcagg cccagcagac gcctcaaagt cagtccttaa gaacaatcgg
      241 tcatcattga aaggagactt taccgatagt gctgagctac atcttgttct gcacgatata
      301 tcaggcaatc cgatcaaagt ttctgaaggg ctggaatttg tacagtcagg taccaacgtg
      361 ccctatgtgc aagttagtgc aattgactac agtaaaaatt tctcaggcga gtacaaagcc
      421 actgttacag gaggcggaga gggtatcaca acgctgatcc ctgtattgaa tggtgttcat
      481 caagctggtc tgagtaccac aatacaattc actcgcgcag aagacaaaat aatgagcggt
      541 acagtatcag tcaatggtac tgacctaccg acaactacat tcccttcgca ggggttcacc
      601 ggggcgtatt atcagttgaa taatgacaac tttgccccag gaaaaacggc ggctgattat
      661 gagttttcaa gctctgcctc ctgggtcgat gttgatgcta ccggtaaagt gacatttaaa
      721 aatgtcggca gcaattcgga aaggattacg gcgacaccaa aatcaggagg ccctagctat
      781 gtatacgaaa tccgtgtgaa gagttggtgg gtgaacgccg gcgaggcttt catgatatac
      841 agccttgctg aaaattttt
//
LOCUS       DAJTYZ010000366          844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000366 DAJTYZ010000000
VERSION     DAJTYZ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.371"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="OO596_004437"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="OO596_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6720059.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..>844)
                     /locus_tag="OO596_004438"
     CDS             complement(733..>844)
                     /locus_tag="OO596_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="catalyzes the epimerization of L-alanyl-D-glutamate
                     to L-alanyl-L-glutamate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6720060.1"
                     /translation="ALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
BASE COUNT          185 a          242 c          226 g          191 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcg
//
LOCUS       DAJTYZ010000367          842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000367 DAJTYZ010000000
VERSION     DAJTYZ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.372"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            54..130
                     /locus_tag="OO596_004439"
     tRNA            54..130
                     /locus_tag="OO596_004439"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="OO596_004440"
     tRNA            173..248
                     /locus_tag="OO596_004440"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
     gene            432..>842
                     /locus_tag="OO596_004441"
     rRNA            432..>842
                     /locus_tag="OO596_004441"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          238 a          177 c          232 g          195 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcgca agaaacatct
      421 tcgggttgtg aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga
      481 tgaaggacgt gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc
      541 gatttccgaa tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg
      601 ttaatgaggc gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc
      661 gagattcccc cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg
      721 tgttagtgga agcgtctgga aaggcgcgcg atacagggtg acagccccgt acacaaaaat
      781 gcacatattg tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg
      841 gg
//
LOCUS       DAJTYZ010000368          838 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000368 DAJTYZ010000000
VERSION     DAJTYZ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.373"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            27..>838
                     /locus_tag="OO596_004442"
     CDS             27..>838
                     /locus_tag="OO596_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6720061.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSF"
BASE COUNT          233 a          182 c          196 g          227 t
ORIGIN      
        1 aactgataat cagacaaaat ggtgacatga gggacattgt ggaccctgta ttctctatcg
       61 gtatctcatc attatgggat gagctgcgac atatgccagc aggcggcgtc tggtggttta
      121 acgtcgatcg ccatgaagat gctatcagtc tggcgaatca aacaattgca tcccaggctg
      181 aaaccgcaca cgtcgcggtc attagcatgg acagcgatcc ggcgaaaatc tttcaattag
      241 atgattctca agggccggaa aaaataaaat tattttcaat gctaaatcat gaaaaaggtc
      301 tatactattt ggcccgtgat ttgcagtgtt ctattgatcc ccataattac ctttttattc
      361 ttgtttgcgc aaataacgca tggcaaaaca ttcctgccga gcggcttcgc tcatggttgg
      421 ataaaatgaa taaatggagc aggttaaacc attgttcgct tttggtaatt aatcccggaa
      481 ataataacga taaacaattt tcattgttgc ttgaggaata ccgttcactt tttggtcttg
      541 ccagtttgcg ttttcagggt gaccaacatt tgctggatat tgccttctgg tgcaacgaaa
      601 aaggggtcag cgcccgtcag cagcttagcg ttcagcaaca aaatggtatc tggacattag
      661 ttcaaagcga agaggcggag atccaaccac gcagcgacga aaaacgcatt ctgagtaatg
      721 ttgctgtact ggaaggtgcg ccgccgctat cggaacactg gcaactgttc aacaataacg
      781 aagtcctgtt caatgaagcc cgtaccgctc aggcggcgac ggtggtcttt tctttcca
//
LOCUS       DAJTYZ010000369          817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000369 DAJTYZ010000000
VERSION     DAJTYZ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.374"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..395)
                     /locus_tag="OO596_004443"
     CDS             complement(<1..395)
                     /locus_tag="OO596_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR6720062.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVS"
     gene            complement(442..648)
                     /locus_tag="OO596_004444"
     CDS             complement(442..648)
                     /locus_tag="OO596_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720063.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT          230 a          208 c          166 g          213 t
ORIGIN      
        1 gaaacccggc acatacggcc ttcagtgccg gagtgagaaa cggtcacctg cccgtcaata
       61 cagccctgca tttcaccgaa ctgcggatgg atcagcgtcc ccggcccggc ggtttcaatc
      121 gctgtaatca gcctgtcgcg ctgctctgcg taatcatcac caacgagata cgcattaatc
      181 gtcagccgtc gcgtggcacg cccgagatct tccgtgtacg gtttgtcacg gtttgggtat
      241 tcatggacct gaacgcggcg tccgaacgaa ccctcgtcgc tctccaccga aaacggaacg
      301 ccacgaaatg aagcgtcgta aagattatcg cgccaattga tacctgaaga tgcagaaaag
      361 agtgaagaaa gagaaggtaa agaaggtaag tccatactgt ctccactata aaaaaaccgc
      421 caatttctgg cggcttatat attaatcatc attaatatct ttcatattca atttgcaatt
      481 ttcgtaggtc actcccgact cgccattaac attaactcca gctctgatat tcccgttttc
      541 ttttacatct acaaaaaact ggcctcttgg catcttgaac gcaaatgtaa aaccaacagt
      601 atctgaatca aaatcatcat ctttatatat tgggatcaaa aatgtcatcg tcgctggtga
      661 tgcattgttt gcaaaatcaa atacacctat acttttgccg aattcataag aagcttttct
      721 caatataatc gcaccgctgg cagaagggga tgggcacgat atcctcagtt gtctgaatcc
      781 atctgtatcc aagtcaacgt cattatctgc agatgtt
//
LOCUS       DAJTYZ010000370          816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000370 DAJTYZ010000000
VERSION     DAJTYZ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.375"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..517)
                     /locus_tag="OO596_004445"
     CDS             complement(<1..517)
                     /locus_tag="OO596_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6720064.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          265 a          171 c          150 g          230 t
ORIGIN      
        1 atgggttgct ggcatcattg cctttcgcat cacccggaca gttagaagag gctacccacc
       61 aagcattttt atataccgca ccattaaaga cgacatacca ggtctgaccg ccctgctgtt
      121 tattccaggc aactacagaa gaattagacg gaacagtgcc ctgtgctggt gtagccgaac
      181 cgccattagc cggcgtattg gatgcaggcg tatttgaatt tgaagaagat gagctgccgc
      241 tcttttcgca ggaaagtgta ttgccaaact gactaatttc agcagctgtt gcggtacgct
      301 ttaaacgcca cgggttagtt gcatcatttg ctttcgcttt tcccgggcaa tttgtagaaa
      361 aaccccacca ggcattttca taaattttgc tatcgaaaat aacctgatat ttattaccac
      421 cttgttggtt attccatgct tccattgcca atgctggcag agcggaacac accagcccca
      481 taccaatcat agatttagta aatatattta atttcattat aatcccttgt gacgtaaaaa
      541 ctgcaaaaca aaagtcctac gcaggaatta tttacgtttt acgagaaccc ttcaatatga
      601 attaaagtga ggcattaaaa ataagaaaaa agcctgacaa atgaagaatt ttaaaaacag
      661 aaacattcat atttaaaata ttaaattgaa ttgatatttt aaatatgaat aatttattcg
      721 ttctgacagt acgaataaga tatgcagtca acaaatgcaa aaagggcgcc gaagcgccct
      781 tttcaattca aaactaatta acgtgtaatt agccca
//
LOCUS       DAJTYZ010000371          794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000371 DAJTYZ010000000
VERSION     DAJTYZ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.376"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            7..114
                     /locus_tag="OO596_004446"
     CDS             7..114
                     /locus_tag="OO596_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6720065.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            490..597
                     /locus_tag="OO596_004447"
     CDS             490..597
                     /locus_tag="OO596_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6720066.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          203 a          184 c          241 g          166 t
ORIGIN      
        1 ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc
       61 attgctggca ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc
      121 atgtgggcgt caggctgccg taatggcaat ttgcgcccgg accaggccgc aggagtgaaa
      181 ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa gcggataata ttctgcggaa
      241 tgcgcagcac agagtgaaga tgagacggat tccggaacag aaaaacagtc gtagaatcag
      301 cgtcaagccg gaaggattcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca
      361 ttggttgctg gagagagaaa acccccgcac gttgcaggta tgcacctgac aacaccacgg
      421 gggccattct tgcatccaaa caatacaaga atagccgcga aacgttgtca ttacaataca
      481 ggcggctata tgacgctcgc agaactgggc atggccttct ggcatgattt agcggctccg
      541 gtcattgctg gtattcttgc cagtatgatc gtgaactggc tgaacaagcg gaagtaacgt
      601 gccatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggatcaggc cgcaggggtg
      661 aaactctgcg gcctttttcg taagaaaaat gcaacggttg caagcggata atattctgcg
      721 gaatgcgcag cacagagtga agacgtgacg gattccggaa cagaaaaaca gtcgtagaat
      781 cagcgtcaag ccgg
//
LOCUS       DAJTYZ010000372          770 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000372 DAJTYZ010000000
VERSION     DAJTYZ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.377"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>770
                     /locus_tag="OO596_004448"
     CDS             <1..>770
                     /locus_tag="OO596_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCR6720067.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDG"
BASE COUNT          278 a          111 c          128 g          253 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc
//
LOCUS       DAJTYZ010000373          754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000373 DAJTYZ010000000
VERSION     DAJTYZ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.378"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..173)
                     /locus_tag="OO596_004449"
     CDS             complement(<1..173)
                     /locus_tag="OO596_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6720068.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVL"
     gene            complement(431..>754)
                     /locus_tag="OO596_004450"
     CDS             complement(431..>754)
                     /locus_tag="OO596_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6720069.1"
                     /translation="EQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDV
                     VMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLA
                     GSGLS"
BASE COUNT          176 a          210 c          130 g          238 t
ORIGIN      
        1 agcaccttcg cacccagcac cgggttcacc gggttagcat aggtaatccc tcccggacac
       61 accgaacagg ccactcccgt cggcgcacct atcagcacac ccgctgaacc ctgcacaata
      121 tccagtccct tgcgggtcat gtcgccctgc cgcgccgccg gttttccgct catggtttat
      181 tcctcttcat ctgaattgat ttacattagg ttggttattt tctcataccc acaactatat
      241 tttctctctg aatttattac gtgcaagaca atcgcagcac tctaactaac ttcgatgcaa
      301 tttgatgttc agaaaaatcc ctcattagat tattatcatg aaatacagct aatcatcttt
      361 agcttatata tttctcaatg tattcttttg aaaggatccg tgccctttca aactcttcag
      421 cagggcaaga ttacgaaagc ccgctccccg caaggactga cgccagatag tttctgtcca
      481 tggctgcttt tcgcatctta cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa
      541 tattaatagc aatgtgccgt atccctgaaa ataattctgc tgcatttcct cttcttattt
      601 tgcagtcgtc ttcattcatt accacgtcca gacgccagtg cagcttattc tccacgtgcc
      661 agtggtttcg gattgctgtg gcgaactttt ctgcagttaa atcagcagaa ctgatataat
      721 atctgaccgt catttctggc tctttctttt gttc
//
LOCUS       DAJTYZ010000374          742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000374 DAJTYZ010000000
VERSION     DAJTYZ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.379"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..395)
                     /locus_tag="OO596_004451"
     CDS             complement(<1..395)
                     /locus_tag="OO596_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6720070.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSR"
BASE COUNT          233 a          171 c          108 g          230 t
ORIGIN      
        1 aacctggacc atgctaaatt cacaccgtgc tgcgcggcct gattaaatag tgcatacgct
       61 ttggcataat ccacccgcac ctgctcaccg tcgtagtaga taccccccag cgctaacgga
      121 gcttctctgt caccttgttt agccgctaac tcgaaccatt taatcgcttc ctgaacatca
      181 ggtttggtat cataacgagg ctgataatat tcccagccta acaggctctg cgcgtcgata
      241 tctcccgatt cagccatttt atgcagtgta tttataacat cgggagcagg tttaccaccg
      301 tatatgctgt catacaattc atgcgctttc tcgctggcca ccacgcttgg tgaaactatc
      361 agcattacag aaaataatat tcttttagtt aacatgtata cctttatgtc ctcactgtca
      421 tatatttcca taataattaa ccaccaaata tccatttaat gaataaatac agtgccataa
      481 aggtccaagt aataagcaca catttagcct gaaacattat cattcatgtc accaaatcgt
      541 ttatttagtg agataaaact agcatattcg taagataaaa aatgcacatg gcaatcaaga
      601 aatgatatta ttactttttt gatgtaaaac tatttagttt taaaattcat tttttcaaca
      661 tatagagcat tcattttatg aatgcaaaaa aacatacctg acacacaaat tactctcctc
      721 tgtaatattt tctcattaac ac
//
LOCUS       DAJTYZ010000375          732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000375 DAJTYZ010000000
VERSION     DAJTYZ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.380"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>732)
                     /locus_tag="OO596_004452"
     CDS             complement(<1..>732)
                     /locus_tag="OO596_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR6720071.1"
                     /translation="AKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVF
                     GHNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAET
                     FTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQ
                     ALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYN
                     PLSGYIANWNNSPQKDYPASDLFAFL"
BASE COUNT          194 a          196 c          155 g          187 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccaacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg
      661 cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat
      721 gattgctttc gc
//
LOCUS       DAJTYZ010000376          726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000376 DAJTYZ010000000
VERSION     DAJTYZ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.381"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..726
                     /locus_tag="OO596_004453"
                     /pseudo
     CDS             1..726
                     /locus_tag="OO596_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          171 a          195 c          198 g          162 t
ORIGIN      
        1 ctcgttcccc aggaaccgct gcttttccca agcctgtcga taaaagaaaa catcctgttt
       61 gggctggcaa aaaaacagct ctccatgcag aaaatgaaga acttgctggc ggcgctgggc
      121 tgctagtttg atctgcatag tctggcagga tcgctggatg tcgccgatcg ccaaatggtg
      181 gaaatcctcc gcggcctgat gcgcgactcg cggattctga tcctcgatga acctaccgcc
      241 tcgcttaccc ctgcggaaac cgaacgcttg tttcgtcgct tgcaagagct gcttgctact
      301 ggcgtgggta ttgtttttat ctcgcataag ctgccggaaa ttcgccagat tgccgatcga
      361 attagcgtga tgcgcgacgg aaccatcgcc ttaagcggca aaaccagcga actgtctacc
      421 gatgacatta ttcaggccat cacgccagtg gtacgggaaa aatcgctctc tgccagccaa
      481 aaattatggc tggagttacc tggcaaccgc ccacaacatg ccgccggaac gtcggtgctg
      541 acactagaaa atctgaccgg cgaaggtttc aggaatgtca gcctgacgct caatgccgga
      601 gaaattctgg gtctggccgg gctggtgggg gccggacgca cagaactggc cgagacgctc
      661 tatggtctgc gtactttgcg tggcggacgc attatgctga atggtaaaga gatcaataaa
      721 ttatcc
//
LOCUS       DAJTYZ010000377          724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000377 DAJTYZ010000000
VERSION     DAJTYZ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.382"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..196
                     /locus_tag="OO596_004454"
     CDS             <1..196
                     /locus_tag="OO596_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720072.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="OO596_004455"
     CDS             207..374
                     /locus_tag="OO596_004455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720073.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            482..715
                     /locus_tag="OO596_004456"
     CDS             482..715
                     /locus_tag="OO596_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720074.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
BASE COUNT          214 a          144 c          192 g          174 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg agatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctg
//
LOCUS       DAJTYZ010000378          709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000378 DAJTYZ010000000
VERSION     DAJTYZ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.383"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>709)
                     /locus_tag="OO596_004457"
     CDS             complement(<1..>709)
                     /locus_tag="OO596_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR6720075.1"
                     /translation="VINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLPWAL
                     MKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCVLTL
                     SLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTVFFI
                     STKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKL
                     DTTYCIKYAILAFLALLP"
BASE COUNT          288 a          154 c          102 g          165 t
ORIGIN      
        1 aggtaataaa gccaaaaatg cgagaatggc atattttata caatatgtag tatctaattt
       61 caccttgaaa cgatgtatac cgaaactggt attactccag agaaaactca ttaccagact
      121 atataaagtg ccattaaaaa taccaatgaa aacaatacaa actattgtca tcagaaccac
      181 agatataata acgctacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac
      241 agtccccatc ccaagggcca ttaaaatcgg caagaaaaag gtcacccacc agaacccttt
      301 catagagcaa ttaaaactaa atcttacgcc gttgagactg gtcatcaagg cctgataacg
      361 cagtcctttt gctgccataa atataagcaa aaccaacagt gacaaagtca aaacacagcc
      421 aataagcggc atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac
      481 aagacaacta acaaaaacat tcccaccggt aatcccataa gaaaatcgtt gtccgttaac
      541 ttccatatta gcatagagat aacgcttaca tttcattaat gcccatggta aatagatacc
      601 tagcgttata atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt
      661 accatgaaaa acaaacgaat gggacggaac atccatatca ttaataact
//
LOCUS       DAJTYZ010000379          706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000379 DAJTYZ010000000
VERSION     DAJTYZ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.384"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            25..591
                     /locus_tag="OO596_004458"
     CDS             25..591
                     /locus_tag="OO596_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6720076.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
BASE COUNT          151 a          178 c          175 g          202 t
ORIGIN      
        1 cgcaaattcg tattggatgc acttatgtca ttcacaaata cccctgaacg ctatggtgtt
       61 atatctgcgg ccttccattg gctaagcgca attattgtct acggcatgtt tgctttaggt
      121 ctgtggatgg tcacgctcag ttattacgat ggctggtatc acaaagcacc cgaactgcat
      181 aaaagcatcg gtattttgtt aatgatgggg ctggttattc gcgttctgtg gcgtgtcata
      241 tctccaccgc cgggtccgct gccgagttat tcgcccatga ctcgtcttgc cgcaaaggct
      301 ggacatctcg cattgtacct attgctgttc gccatcggca tcagcggcta tctgatctca
      361 actgccgatg gtaaaccgat cagcgttttt ggctggtttg acgtccccgc gacccttgcc
      421 gacgctggcg cacaggcaga ctttgccggt gccctgcatt tttggctcgc gtggagcgtc
      481 gtagtactgt ccgttatgca cggatttatg gccctgaagc atcatttcat cgataaagac
      541 gacactctga agcgcatgct gggaaagtcg tcatctgact atggagtata aaaaatgaaa
      601 aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc
      661 gccgattaca aaattgacaa agaaggtcag cacgcctttg ttaatt
//
LOCUS       DAJTYZ010000380          701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000380 DAJTYZ010000000
VERSION     DAJTYZ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.385"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            71..146
                     /locus_tag="OO596_004459"
     tRNA            71..146
                     /locus_tag="OO596_004459"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
     gene            340..>701
                     /locus_tag="OO596_004460"
     rRNA            340..>701
                     /locus_tag="OO596_004460"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          202 a          153 c          189 g          157 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtccccttcg tctagaggcc caggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctggatc
      241 aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt gagtctctcg aattttcgca
      301 acacgatgat gaatcgaaag aaacatcttc gggttgtgag gttaagcgac taagcgtaca
      361 cggtggatgc cctggcagtc agaggcgatg aaggacgtgc taatctgcga taagcgtcgg
      421 taaggtgata tgaaccgtta taaccggcga tttccgaatg gggaaaccca gtgtgtttcg
      481 acacactatc attaactgaa tccataggtt aatgaggcga accgggggaa ctgaaacatc
      541 taagtacccc gaggaaaaga aatcaaccga gattccccca gtagcggcga gcgaacgggg
      601 aggagcccag agcctgaatc agtgtgtgtg ttagtggaag cgtctggaaa ggcgcgcgat
      661 acagggtgac agccccgtac acaaaaatac acatattgtg a
//
LOCUS       DAJTYZ010000381          695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000381 DAJTYZ010000000
VERSION     DAJTYZ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.386"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            109..>695
                     /locus_tag="OO596_004461"
     CDS             109..>695
                     /locus_tag="OO596_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6720077.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVG"
BASE COUNT          186 a          148 c          149 g          212 t
ORIGIN      
        1 attaatgcag taactatatt ttcctttctg gtgataacgt cgggttatca ttagcttctt
       61 cagctatttt acttttatat cccttgtaaa catcatggag aatccatagt gaataaagtt
      121 tattctctta aatattgccc cgtcaccggg gggcttattg ctgtctctga acttgcccgc
      181 agggtaataa aaaagacatg ccgaagatta acgcatattc ttctggctgg cattccagca
      241 atctgtctgt gttactctca gatatccctg gcgggtattg tccgttccga tattgcctat
      301 cagatttatc gtgatttcgc cgaaaacaaa gggctttttg tacctggtgc caatgatatt
      361 ccggtatatg ataaggacgg aaaacttgtg ggaagactgg gtaaagcccc aatggccgat
      421 ttcagcagtg tgagctcaaa tggcgttgct acgcttgtat cgcctcagta tatcgtcagc
      481 gtaaagcata acggaggata tcggagtgtg agctttggta atgggaaaaa tacatattcc
      541 cttgttgacc gtaataacca cccttctatt gacttccatg ctccacgtct gaataaactg
      601 gttacagaag ttattccctc agcggtaaca tcagaaggaa ccaaagccaa tgcttataaa
      661 tacactgaac gttacaccgc tttttatcgg gtggg
//
LOCUS       DAJTYZ010000382          690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000382 DAJTYZ010000000
VERSION     DAJTYZ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.387"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          239 a          106 c          141 g          204 t
ORIGIN      
        1 gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga agtaccttag
       61 aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata agtggcagta
      121 agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg gaggattcat
      181 tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga ttcttagaag
      241 ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg aaagtccctt
      301 attaagtagg agtcttaata gactgtctta atagataact taaaagtctt aaaggatatc
      361 ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt gaacagactg
      421 tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata ctagcagttt
      481 gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact ttttgttaaa
      541 acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca tgttcttggg
      601 ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag taacaaccga
      661 agaacgagga tagaggtatt gtaataactt
//
LOCUS       DAJTYZ010000383          685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000383 DAJTYZ010000000
VERSION     DAJTYZ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.388"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            10..453
                     /locus_tag="OO596_004462"
     CDS             10..453
                     /locus_tag="OO596_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6720078.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
BASE COUNT          219 a          137 c          164 g          165 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggtgatttca ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt
      421 gacggacgag ggagggaatg gaaagacatt taaaattaat ttcgaatgat ttcgaaacac
      481 cacttgtaat taattgatta taaatgatta attcctaatg ggcatttgct tcatgaatgc
      541 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
      601 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
      661 aattcttaaa tgacgcggta aaagc
//
LOCUS       DAJTYZ010000384          682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000384 DAJTYZ010000000
VERSION     DAJTYZ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.389"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            95..>682
                     /locus_tag="OO596_004463"
     CDS             95..>682
                     /locus_tag="OO596_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6720079.1"
                     /translation="MPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLW
                     ILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQL
                     AAKLAQQGICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAG
                     NLVLISPFYPEVRFTVGNAMARNKYIYCLAESAMVV"
BASE COUNT          156 a          146 c          207 g          173 t
ORIGIN      
        1 ggcggcgagt gagtataaac ctcttagtaa tagtgaatgg gggaagtttg cattatggct
       61 gaagcaccaa cgtatcagtc ccgccgagct tctggtgccg caaccgcaag agaaacttac
      121 aggctggagc gatccgcgta tttctcagga gcgtattctt ggcttgctgg cgcgtggtca
      181 tagtctggcg ttggcggtag ataagtggca acgcgccggt ttatggatct taacccgcgg
      241 agatgctgat tatcccgttc gcttgaaaaa ccgattgcga acggatgcac ctcccgtttt
      301 atttggctgc gggaataaag cattactgca agcggaaggt atggcgattg ttggctcgcg
      361 agatgctccg actgacgatt tgcgctatac ccaacaactg gccgcgaaac tggcccaaca
      421 ggggatttgc gttatctctg gtggtgcgcg aggtattgat gaatgtgcaa tggcgtcggc
      481 actggaggcc gggggaactg ccgttggcgt attagctgat agcttgttaa aaacgagtac
      541 gttagtgaaa tggcgtgaag ggcttatagc aggcaacctg gtgttgattt cgccgtttta
      601 cccagaggta cgtttcaccg tcggcaatgc gatggcgcga aataaatata tttattgcct
      661 tgctgaaagc gcaatggttg ta
//
LOCUS       DAJTYZ010000385          679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000385 DAJTYZ010000000
VERSION     DAJTYZ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.390"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..173
                     /locus_tag="OO596_004464"
     CDS             <1..173
                     /locus_tag="OO596_004464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720080.1"
                     /translation="KKLVFWFVRVDDEGYPEIARCMEREFATIPAGISADGMYCPECG
                     TVHWPDGVIPPF"
     gene            221..650
                     /locus_tag="OO596_004465"
                     /pseudo
     CDS             221..650
                     /locus_tag="OO596_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          159 a          139 c          230 g          151 t
ORIGIN      
        1 acaaaaaact ggtcttctgg tttgtacgtg ttgatgacga agggtatccg gaaattgccc
       61 gctgcatgga gcgggaattt gcgacaattc ctgccggtat cagcgccgac ggcatgtatt
      121 gccctgaatg cggtacggtt cactggccgg atggcgttat tccgcctttc tgatgcttcc
      181 ccgttttgcc gatatttttc agcagggaaa ccgatggctt aactggctgg agaagcagtc
      241 ggtgcagatg agccgcctgg aacattatgc cggtcaggat gaaattggcc tgcgttacaa
      301 cagccaccgg acaaaacgag aagaaaacct tgtgatgagt ggcgacgagt ttatggaaag
      361 gttctcctgg catgtggcgg ataaggggtt ccggatggtg atacggggtc ctgagtccgg
      421 tgaagcggcg attactggaa gatgtggtgt acgccataac ggagacagtg agaaaaacgg
      481 cgaggcaaat cacaaggaga gggatgtatc agcagtgact gaaggttgac ccgctgaagt
      541 gcgagctgtg cggaggtcag atgcggttta cggggctgaa gaggggttac cgtctggcag
      601 agcaggttat gatgcatgag ccgctggctc gaatacggtg gtgcggctga gagtcgcaga
      661 ggggaagttg cgtccatgt
//
LOCUS       DAJTYZ010000386          677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000386 DAJTYZ010000000
VERSION     DAJTYZ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.391"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..361
                     /locus_tag="OO596_004466"
                     /pseudo
     CDS             1..361
                     /locus_tag="OO596_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DAJTYZ010000387          672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000387 DAJTYZ010000000
VERSION     DAJTYZ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.392"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..217
                     /locus_tag="OO596_004467"
     CDS             <1..217
                     /locus_tag="OO596_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6720081.1"
                     /translation="AEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRET
                     EQQIYRQLTDEVLALRLSENGSQLHHS"
     gene            complement(218..397)
                     /locus_tag="OO596_004468"
     CDS             complement(218..397)
                     /locus_tag="OO596_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720082.1"
                     /translation="MNKPQLAESQKTALLTEAESVIRPRGRAVRLNRETDESGEARGR
                     ASVFPESVRSVVYAM"
     gene            complement(397..>672)
                     /locus_tag="OO596_004469"
     CDS             complement(397..>672)
                     /locus_tag="OO596_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6720083.1"
                     /translation="GRIWNAGPNSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
BASE COUNT          155 a          198 c          178 g          141 t
ORIGIN      
        1 ggcagaagag cagaaatatg agatgctgga gaatgagtac cctcagaggg tggctgaccg
       61 gctgaaagca tcaggtctga gcggtgatgc ggatgcggag agggaagccg gtgcacaggt
      121 gatgcgtgag actgaacagc agatttaccg tcagctgact gacgaggtac tggccctgcg
      181 attgtctgaa aacggctcac aactgcacca ttcataatca catcgcataa accacagacc
      241 ggactgactc cggaaaaaca gaggcccgcc cccgggcctc cccggattca tccgtttccc
      301 tgttcagcct gacagcacgc ccccgcggcc ggatgacaga ctccgcttcg gtaagcaaag
      361 cggtcttctg tgattccgcc agttgcggct tattcattac tcaacgtcaa acgcccgaat
      421 tgaagccaaa tcatccagac cgctcagctc ctctttcatt tcacgctgac ggcgataaat
      481 ctcatcgttg cgatcgacct gcgcctgcac cattgctgcc gccagttctt ccagttccgg
      541 catcgacagt ttcacctgct gattatcggc atcgctccac gccatatgtg tttgtgctgt
      601 gacagatttt gccagcatga ctaccgggga caggcggccc agtgagttgg ggccagcatt
      661 ccagatacga cc
//
LOCUS       DAJTYZ010000388          669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000388 DAJTYZ010000000
VERSION     DAJTYZ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.393"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..647)
                     /gene="ipaH9.8"
                     /locus_tag="OO596_004470"
     CDS             complement(<1..647)
                     /gene="ipaH9.8"
                     /locus_tag="OO596_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6720084.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNP"
BASE COUNT          194 a          112 c          158 g          205 t
ORIGIN      
        1 aatgggttat cactaatatg tattgaacat tcattcctca gattaagaat gctttccggg
       61 atatgactta tctgatttct atcaaaaaaa tactctctga caacgggatt atttccctca
      121 gaaaatgctg gtagctcagt gaggaaatta cgggtcgctc tgagattttt aagagcctgt
      181 gggagcgatg gtaaggagac tatttcgtta tagctgatat tcatagtcaa taacgaatcg
      241 ggtaaagctg gcaggttttc cagctcattg tgttgtacct gaagtgactc cagcgcagga
      301 ggtagcacgg gcaattctga taatttattg ctggcggaat ataatttttt tagcgtaaca
      361 ggcagttcag gtaggttagt taattgatta tatgatacat tgagcagcgt tatctgagct
      421 ggtaagttgt caggtagcga ggacagattt aaacggtcca gtcgaagttc atcggaatta
      481 ttgataagac attctttaag tcgggagaca gcctcatcac gctcttcacc ggggagcgct
      541 tgtttttccc atttatccca tgctgaaaag taatcagcat atgtaccgga aatagtgtta
      601 taaaaagaat tttggggcaa tgaaaagtta ttatttatcg gtaacatgat gggcatccag
      661 ttaaagccg
//
LOCUS       DAJTYZ010000389          663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000389 DAJTYZ010000000
VERSION     DAJTYZ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.394"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..256
                     /locus_tag="OO596_004471"
     CDS             <1..256
                     /locus_tag="OO596_004471"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6720085.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            315..>663
                     /locus_tag="OO596_004472"
     CDS             315..>663
                     /locus_tag="OO596_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6720086.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLF"
BASE COUNT          155 a          169 c          158 g          181 t
ORIGIN      
        1 gaccgaagaa gtttacctcg acgaaatggt aggtcgtatt aacgccaata tgatccttcc
       61 gtatccgccg ggagttcctc tggtaatgcc gggtgaaatg atcaccgaag aaagccgtcc
      121 ggttctggag ttcctgcaga tgctgtgtga aatcggcact cactatccgg gatttgaaac
      181 cgatattcac ggtgcatacc gtcaggctga tggccgctat accgttaagg tattgaaaga
      241 agaaagcaaa aaataattag ctcgtacaag ggaagtggct tgccacttcc cttttttgct
      301 cataaggaga acacatgaaa acaccctcac agccgcgcgc gatatactat atcgtggcga
      361 tccaaatctg ggagtacttc agtttttacg gcatgcgtgc cttactcatt ctctatctca
      421 cccatcagct tggttttgat gataaccatg ccatcagcct gttcagcgca tatgcttctc
      481 tggtttacgt tacccctatt ctcggcggct ggcttgccga ccgcctgctc ggcaaccgca
      541 ctgcagtgat tgccggcgcg ctgttaatga cccttggcca tgtggtgctg ggcattgata
      601 caaattcaac ctttagcctg tatctggcgc tggcaatcat tatttgtggc tacggtttat
      661 tca
//
LOCUS       DAJTYZ010000390          657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000390 DAJTYZ010000000
VERSION     DAJTYZ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.395"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>657
                     /gene="nlpA"
                     /locus_tag="OO596_004473"
     CDS             <1..>657
                     /gene="nlpA"
                     /locus_tag="OO596_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR6720087.1"
                     /translation="KVGVINGAEQDVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATN
                     HGELDANVFQHRPFLEQDNQAHGYKLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAI
                     PNDPTNLGRALLLLQKEKLITLKEGKGLLPTALDITDNPRHLQIMELEGAQLPRVLDD
                     PKVDVAIISTTYIQQTGLSPVHDSVFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQ
                     S"
BASE COUNT          200 a          136 c          165 g          156 t
ORIGIN      
        1 aaagttggcg ttataaatgg cgcagaacaa gatgtcgcgg aagtcgctaa aaaggtggca
       61 aaagagaaat atggcctcga tgttgaactg gtaggattta gcggttcgtt gctaccgaac
      121 gatgcgacaa atcatggcga acttgatgcg aatgtcttcc agcatcgacc ctttcttgaa
      181 caagataatc aggcgcatgg ttataaactg gtagcgctgg gaaatacgtt tgtcttccct
      241 atggcgggtt attccaaaaa aatcaaaacc gtggcgcaaa taaaagaagg cgcgacagtg
      301 gcgatcccca acgacccgac caaccttggt cgcgcacttt tactgttgca aaaagagaag
      361 ctaattactc tgaaagaggg aaaagggtta ttacctaccg cgctggatat aaccgataat
      421 cctcgtcatt tgcagattat ggaactcgag ggggcgcagc tcccgcgcgt actggatgat
      481 cccaaagttg atgtagcgat tatcagcacc acttacattc agcagacagg gctttctccg
      541 gtgcacgaca gcgtatttat tgaagataag aattcgccgt atgtgaatat tttggtggca
      601 cgggaagata ataagaatgc agaaaacgtg aaggaatttc tgcaatctta tcaatca
//
LOCUS       DAJTYZ010000391          637 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000391 DAJTYZ010000000
VERSION     DAJTYZ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.396"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>637
                     /locus_tag="OO596_004474"
     CDS             <1..>637
                     /locus_tag="OO596_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6720088.1"
                     /translation="KQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGK
                     AASAKLIRSTLSDAFREAIAEGHITTKPVAATRAAKSEVRRSRLTADEYLKIYQTAES
                     SPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALG
                     ISMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDP"
BASE COUNT          213 a          136 c          149 g          139 t
ORIGIN      
        1 aagcagaaga cactcataaa ttacatgagc aaaattaaag caataaggag ggggctgcct
       61 gatgctccac ttgaagacat caccacaaaa gaaattgcgg caatgctcaa tggatacata
      121 gacgagggca aggcggcatc agccaagtta atcagatcaa cactgagcga tgcattccga
      181 gaggcaatag ctgaaggcca tataacaaca aaaccggtcg ctgccactcg cgcagcaaaa
      241 tcagaggtaa ggagatcaag acttacggct gacgaatacc tgaaaattta tcaaacagca
      301 gaatcatcac catgttggct cagacttgca atggaactgg ctgttgttac cgggcaacga
      361 gttggtgatt tatgcgaaat gaagtggtct gatatcgtag atggatatct ttatgtcgag
      421 caaagcaaaa caggcgtaaa aattgccatc cctacaacat tgcatgttga tgctctcggg
      481 atatcaatga aggaaacact tgataaatgc aaagagattc ttggcggaga aaccataatt
      541 gcatctactc gtcgtgaacc gctttcatcc ggcacagtat caaggtattt tatgcgcgca
      601 cgaaaagcat caggtctttc cttcgaaggg gatccac
//
LOCUS       DAJTYZ010000392          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000392 DAJTYZ010000000
VERSION     DAJTYZ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.397"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>635)
                     /gene="torZ"
                     /locus_tag="OO596_004475"
     CDS             complement(<1..>635)
                     /gene="torZ"
                     /locus_tag="OO596_004475"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6720089.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKP"
BASE COUNT          133 a          179 c          150 g          173 t
ORIGIN      
        1 ctggtttgcc agattttttc agctgatgga agtattccag cccttgctca tcggtactgc
       61 tccaggcaat ttttaaggta ttcagcgggt tcatccccca cagcaccaca acctggctgt
      121 tttccagaat cagcggccag gaggtctgct gttcatacac ctctacagaa ccgaccacat
      181 gcggcatgat cacctgtgcc gccccggttg aataatcgcc gctatgcccg gaataaccgc
      241 ccgccaggtt catataacgt tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc
      301 gccagccgta tgatccggca aaaatagccg atggtccgtt agctttgcga atgcgatcgt
      361 gttgctcatg aatcagtttt aatgcttgtt cccagctcac ctgtacatag gtatcttcgc
      421 cacgaccttt cgtcggttgc agtggattat cgagatagct ttttctcacc atcggatgct
      481 gaatacgcgt cgtggtgtgt acctgatccg ccgccgtaga ctgtaaggaa ttcggtatgg
      541 ttttcgccag cgcgcctgtt gaagaaacaa tcttgccgtc cttcacttct acgttcatcg
      601 ctccccaacg ttccgcggtg aggattttac cgtcc
//
LOCUS       DAJTYZ010000393          613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000393 DAJTYZ010000000
VERSION     DAJTYZ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.398"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..137)
                     /locus_tag="OO596_004476"
     CDS             complement(<1..137)
                     /locus_tag="OO596_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HCR6720090.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     T"
     gene            complement(155..>613)
                     /locus_tag="OO596_004477"
     CDS             complement(155..>613)
                     /locus_tag="OO596_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6720091.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
BASE COUNT          148 a          145 c          138 g          182 t
ORIGIN      
        1 ttagttggat agggtttgtg gatttttgct ggctgacggg caaataaagt ttgtttaatg
       61 gtttgtttat ctgccagttg attaatcaca tggcgggcta tctgaccgcc agcaccaaga
      121 atcagtacat ttttcataat atttctcatg aaacttatcc cagatgggtg ttgaaaaaag
      181 gaataacttt ttccagcgcc tcctgaactg cttccggttt gtcatacagt tcataatgtg
      241 agaaatcggg taatgaaact aactgacgat cctgcgaaac catcgcccgg ccatagactt
      301 ccagtccatc acgataagca ccgaaggcac cgactttttc accaactact accatcaccg
      361 gctgagtcag taacacttca gtaaatgcga aggcatccca ggccagcgtt ttctgtgcat
      421 gtgagaacaa catttttgtc gcacctcccg gttgttgccc gcgtggtgtt ttatagtagt
      481 cagttgcttc gtaaacatcg cgttcggtca gtccatgggc ttttgctgca tccaggctgg
      541 cgggaagtaa ttcgttgatc tgtaattctc caccacgagc ctctttggtt cgttgcgacg
      601 ccattgcatt caa
//
LOCUS       DAJTYZ010000394          604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000394 DAJTYZ010000000
VERSION     DAJTYZ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.399"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..545)
                     /gene="cadA"
                     /locus_tag="OO596_004478"
     CDS             complement(<1..545)
                     /gene="cadA"
                     /locus_tag="OO596_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCR6720092.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDIS"
BASE COUNT          185 a          129 c          128 g          162 t
ORIGIN      
        1 atggaaatat cagatttcat ggtattcgga ccaaagaaat catagaacag gctacctacc
       61 gggcttttct ggaatgcagt accgcccatg tgaccaggag tacagaaagt atatttacct
      121 tcacgaacat atttaaacag tgctttagtc agcggaggca gaatagtgtt gatatattcg
      181 tcagtggtct gcttgatctt attagcaata tcttcagcag cacccagcgc atattcaaag
      241 aagctaatct gtaaacgcag gtcattcagg cttacatcga gagtggaata cgtattagcg
      301 aacgcgtaca acggcaggtt ctcgttcatt ttgctaattt cttcgcacag ctcgagatta
      361 tatttatccc agtcaaaaat aacgccgcac agacgcgcat tgttttcgat cagttttaat
      421 aagtcgtcac ggtcgttcgg gtaaacaatc tggaagttca gacgttcaag cgcgcgatga
      481 agttcacgga tgggttcttc tttaaaataa acccccatgt gattcaatat tgcaataacg
      541 ttcatagtca tatctccagg taaaaaaggc ccctcccaac acatgggaca aaatgaaagg
      601 agga
//
LOCUS       DAJTYZ010000395          584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000395 DAJTYZ010000000
VERSION     DAJTYZ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.400"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..422
                     /locus_tag="OO596_004479"
     CDS             <1..422
                     /locus_tag="OO596_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720093.1"
                     /translation="DIKANVQAGRFTGLKKLVQFINSVRTDCKCILLEHHMPLLRLFP
                     KGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLLLSVLDV
                     KTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          173 a          117 c          123 g          171 t
ORIGIN      
        1 ttgatattaa agcaaatgtg caggctggac gctttaccgg gcttaagaaa ttagtgcagt
       61 ttatcaattc agtgcgaaca gattgcaaat gtattcttct tgaacaccat atgcctttgc
      121 tgagactttt ccctaaaggg aaagagtgta tgcatgtgga aaaatggctt cgcgaaatgt
      181 cgtctattcc ggagacagac gcgcccatta agcaggcgct tgcgcatgga ttattattac
      241 acttaaaaaa tatctaccct gtttttcctg aatcactggt gatgttattg ctctctgttc
      301 tggacgtaaa aacatacaga gatgatgcac gattaaatga atggatatct aatagagtgc
      361 aggaattagg tgacagatat tatcctgtaa ataagcacgt taaaatccgc tacactcttt
      421 agttcagaaa gggttccacc tgcaagttgt tattccacca gagagtgaat cgcaaagcag
      481 gtaatcattt catctgtata tccgtatgcc aaatggcatt ccaccgcctt ttcgccgata
      541 ttttgttgtg atcggcgaca cttcgcttaa aaaagcacca gcag
//
LOCUS       DAJTYZ010000396          583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000396 DAJTYZ010000000
VERSION     DAJTYZ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.401"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="OO596_004480"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="OO596_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6720094.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..>583)
                     /locus_tag="OO596_004481"
     CDS             complement(448..>583)
                     /locus_tag="OO596_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720095.1"
                     /translation="EQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPARELTRGQ
                     "
BASE COUNT          142 a          162 c          128 g          151 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcc
//
LOCUS       DAJTYZ010000397          580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000397 DAJTYZ010000000
VERSION     DAJTYZ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.402"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..580
                     /locus_tag="OO596_004482"
                     /pseudo
     CDS             1..580
                     /locus_tag="OO596_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          139 a          154 c          157 g          130 t
ORIGIN      
        1 cgaccttccc gatgaggcat ggaccatcat caagcctctg ttacctcctg aacccgccac
       61 accacgggcc ggacgcccat gggctgagca ccgtaaaatc atcaatggca tgttctgggt
      121 gttatgttcc ggtgcaccat ggcgcgattt acccgaacga tatgggtcat ggaaaactgt
      181 ttataaccgc tttaaccgat ggtcaaagtc aggcgtgatt aatattattt tcaacaggtt
      241 gctttcgcta cttgatgcaa acggctttat tgactggtct gccaccgcgc tggatggcag
      301 taatatccgg gcgctgaaat gtgccgccgg tgctcaaaaa aacatcccga tatcgacgga
      361 gataatgggc cgggtcgctc tcgcggcggt tttggcacca aaatccatct ggcaacagac
      421 ggaagtggcc tcccgttaaa tatcgtgcta agccccggac aagctcatga aagccagttc
      481 gcgcaacgac ttctggacgg gattggcgtt cagcgtcaga acggcagcat gaaacgccgt
      541 ggacatgcgg tactggctga taaagcgtat tcagggcgcg
//
LOCUS       DAJTYZ010000398          577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000398 DAJTYZ010000000
VERSION     DAJTYZ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.403"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>577
                     /gene="espL4"
                     /locus_tag="OO596_004483"
     CDS             <1..>577
                     /gene="espL4"
                     /locus_tag="OO596_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HCR6720096.1"
                     /translation="PQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVC
                     RHIAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAAC
                     GDFGLVMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNV
                     TNDKIRIMSESKEDIKHYSLMDFMNVDYSLLK"
BASE COUNT          199 a          110 c          110 g          158 t
ORIGIN      
        1 cctcaacgct cagacactct tgataaacaa gaattaaacc taaaatcaga tagaaaagaa
       61 aaagaattcc caagaattaa acttaatggt caatgttatt ttccggggcg accccaaaac
      121 cgtatagtat gccgacacat tgctgcacaa tatattaatg atatttatca gaatgttgat
      181 tacaaacccc atcaagatga ttattcttca gctgaaaaat ttctcactca cttcaacaaa
      241 aaatgcaaaa accagacttt ggcgttgatt tccagccgtc ctgaggggcg ttgcgttgct
      301 gcctgcggtg atttcgggct agttatgaaa gcatattttg acaagatgga atcaaatggc
      361 atcagtgtta tggcagccat attactggtg gataaccatg ctttgacggt ccggctaaga
      421 ataaagaaca caactgaagg atgtacccat tacgtggttt cggtttatga tcctaatgta
      481 actaacgaca aaataagaat catgagcgaa agcaaagagg atattaaaca ctattctctg
      541 atggatttta tgaatgtaga ttatagcctc ctgaaat
//
LOCUS       DAJTYZ010000399          574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000399 DAJTYZ010000000
VERSION     DAJTYZ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.404"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..268
                     /locus_tag="OO596_004484"
     CDS             <1..268
                     /locus_tag="OO596_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720097.1"
                     /translation="GLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAE
                     EGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            320..>574
                     /locus_tag="OO596_004485"
     CDS             320..>574
                     /locus_tag="OO596_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6720098.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLV"
BASE COUNT          133 a          141 c          132 g          168 t
ORIGIN      
        1 cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt
       61 ccagttcaat atcagtcgct acagccaaca acaactgatg gaaaccagcc atcgccatct
      121 gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg
      181 cgacgactcc tggagcccgt cagtttcagc ggaattccag cttagcgccg gtagctacca
      241 ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggccatgtc tgcccgtatt
      301 tcgcgtaagg aaatccatta tgtactattt aaaaaacaca aacttttgga tgttcggttt
      361 attctttttc ttttactttt ttatcatggg ggcctacttc ccgtttttcc cgatttggct
      421 acatgacatc aaccatatca gcaaaagtga tacgggtatt atttttgccg ctatttctct
      481 gttctcgcta ttattccaac cgctgtttgg tctgctttct gacaaactcg ggctgcgcaa
      541 atacctgctg tggattatta ccggcatgtt agtg
//
LOCUS       DAJTYZ010000400          565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000400 DAJTYZ010000000
VERSION     DAJTYZ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.405"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..246
                     /locus_tag="OO596_004486"
                     /pseudo
     CDS             6..246
                     /locus_tag="OO596_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     misc_feature    201..317
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            246..395
                     /locus_tag="OO596_004487"
     CDS             246..395
                     /locus_tag="OO596_004487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720099.1"
                     /translation="MPLLDKLRGQYGVGPVCSELHIAPSTYYHCQQQDIILINAVPVR
                     STMTG"
     gene            332..>565
                     /locus_tag="OO596_004488"
     CDS             332..>565
                     /locus_tag="OO596_004488"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6720100.1"
                     /translation="MSATGHHPDKRSARAQHDDWLKREIQRVYDENHQVYGVRKVWRQ
                     LLREGIRVARCTVARLMAVMGLVGVLRGKKVRTT"
BASE COUNT          133 a          135 c          175 g          122 t
ORIGIN      
        1 tcacaatggg cggtaatttg ttccattact ccaaagattg gctgtacacc ggagactctg
       61 cgtgtctggg ttcgccagca tgagcgggat accaggggcg gtgatggagg gctcaccacc
      121 gctgaacgtc agcgtctgaa agagctggag cgtgagaatc gtgaactgcg ctgcagtaac
      181 gatatccttc gccaggcttc cgcttatttt gcgaaggcgg agttcgaccg cctctggaaa
      241 aaataatgcc actgctggat aagctgcgtg ggcagtacgg ggtcggaccg gtatgcagtg
      301 aactgcatat tgccccgtca acgtattacc actgtcagca acaggacatc atcctgataa
      361 acgcagtgcc cgtgcgcagc acgatgactg gctgaagaga gagatacagc gcgtatacga
      421 tgaaaatcat caggtgtacg gtgtgcgtaa agtctggcgc cagttgttac gggaaggaat
      481 cagggtggcc agatgtacag tggcgcgcct catggcggtt atgggacttg tcggtgttct
      541 ccggggtaaa aaggtccgca ctacc
//
LOCUS       DAJTYZ010000401          557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000401 DAJTYZ010000000
VERSION     DAJTYZ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.406"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>557
                     /locus_tag="OO596_004489"
     CDS             <1..>557
                     /locus_tag="OO596_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6720101.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLK"
BASE COUNT          115 a          160 c          157 g          125 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAJTYZ010000402          557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000402 DAJTYZ010000000
VERSION     DAJTYZ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.407"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>557
                     /locus_tag="OO596_004490"
     CDS             <1..>557
                     /locus_tag="OO596_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6720102.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLK"
BASE COUNT          111 a          162 c          159 g          125 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAJTYZ010000403          551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000403 DAJTYZ010000000
VERSION     DAJTYZ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.408"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>551
                     /locus_tag="OO596_004491"
     CDS             <1..>551
                     /locus_tag="OO596_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6720103.1"
                     /translation="VVISKYTDHLPLYRQQHIFSRMGVELPVSTMADMVGVAGAALAP
                     LVKLLRHELLTRDVIHADETSLRLLDTRKGGKSCSGWLCAYVSGERSGPPVVCFDSQT
                     GRALRYPETWLQCWCGGTLVSDGYSVYKSLADNHPGITSACCWSHAGRGFANLYKASR
                     EPRAGVELRKIAGLYRIEKLIRE"
BASE COUNT          114 a          150 c          167 g          120 t
ORIGIN      
        1 aggttgtcat cagcaaatat accgaccatc tgcccctgta tcgccagcag cacattttct
       61 cccgaatggg tgtggagctc cccgtcagta caatggcgga tatggtcggt gttgccggag
      121 ctgcactggc cccgctggtg aaactgctac gccatgagct tctgacacgg gatgttattc
      181 atgcagatga aacgtccctg aggctccttg atacccggaa aggcggtaaa tcctgctccg
      241 gctggctgtg tgcttacgtc agcggtgaaa gaagcggtcc gccggtcgtc tgttttgact
      301 cgcagaccgg acgggccctc agataccctg agacctggct gcagtgctgg tgtggcggta
      361 cgctggtgag tgacggatac agcgtgtaca aatctctggc ggataatcac cccggcatca
      421 catcagcctg ttgctggagt cacgcagggc gcggcttcgc aaatctgtat aaggccagca
      481 gggagccccg tgcaggcgtg gagctgagaa aaatcgcggg tctttaccgc attgagaaac
      541 tcatccgtga g
//
LOCUS       DAJTYZ010000404          551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000404 DAJTYZ010000000
VERSION     DAJTYZ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.409"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..551
                     /locus_tag="OO596_004492"
                     /pseudo
     CDS             1..551
                     /locus_tag="OO596_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          195 a          121 c           84 g          151 t
ORIGIN      
        1 tccgacttat taaaaatgcc tgtttaaaat tatataattt agcacatacc gcaaccaact
       61 cccaccttaa accaactggt ccggataatt cagatgttct cctgttcaaa aagctattct
      121 ccccttcgaa attaatgaca attatcggtg atgaaattcc tcttatatct gaaaaacagt
      181 cgctttcaaa ggtgctttta aatgatgaga ataatgaact gagtgatggt acaaacttct
      241 gggataaaaa tcgtcaatta accacagatg aaatagcttg ctatcttcag aagatcgccg
      301 ctaatgcaaa aaatactcaa gtcaattatc ctactggtct ctacgtcccc tactccacca
      361 gaactcacct ggaagacgct ctcaatgaaa atattaagag cgatccatca tggccgaatg
      421 aagtccagtt attccccata aatactggcg gacactggat attagtttcg ctacagaaaa
      481 tagtaaataa aaaaataata aactacaaat aaaatgcgtc atattcaact cattgcgtgc
      541 actaggccat g
//
LOCUS       DAJTYZ010000405          543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000405 DAJTYZ010000000
VERSION     DAJTYZ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.410"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>543
                     /locus_tag="OO596_004493"
     CDS             <1..>543
                     /locus_tag="OO596_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720104.1"
                     /translation="RLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTSSVALVTSGL
                     ERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITLQLDLGGESP
                     GYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGIKRYEKDNYQ
                     SANFWFNDIKTDTDQNSHAKR"
BASE COUNT          188 a           97 c          114 g          144 t
ORIGIN      
        1 cgattaaaat cggttgcgct gctgattgct tatctggata aagccaattt ctatgttatg
       61 gaagattccg gagcatggga agaagatgcc cgattaaaca catcttccgt tgcactggtg
      121 acctctggac tggaacgttt gtcgaactta ctctcgaaga aagactctgt ttttgtctct
      181 gatttactac gcgaagctaa agccaatgaa ctggatgaac ctctttcaac aacgaggttg
      241 aaccacctca tagataaagg ttatgaaaga ataacgttac agcttgattt agggggagaa
      301 tccccaggtt atctggaaaa agataaacat tacagagaag ccgatgcggc tttacttaat
      361 gttatttatc ctgcaaatct tgcaaagata aacaccagaa gaaaagagca ggttctaaaa
      421 atagttaaaa aactagcagg tccgtatggt ataaaaagat acgagaagga taattatcag
      481 tcggcaaatt tttggttcaa tgatattaaa accgataccg atcaaaattc acatgcgaaa
      541 aga
//
LOCUS       DAJTYZ010000406          539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000406 DAJTYZ010000000
VERSION     DAJTYZ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.411"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..378
                     /locus_tag="OO596_004494"
     CDS             <1..378
                     /locus_tag="OO596_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720105.1"
                     /translation="ANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKKN
                     TGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQA
                     SKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT          153 a          112 c          133 g          141 t
ORIGIN      
        1 gctaacggtc ttcgtattca gatgaccttc caaggtgtag agtttcgttc aatcgtaggt
       61 cagactgatg tttcggctgc tactggaaag accgctacca cgaatgatgg cggtgctacc
      121 agtaaaaaga acaccggaaa cactacaacg agccttggaa gccctatgct gacctgtaaa
      181 gagctttttg gctattcggc aagtgagtta tcagatgaag cgctaaaagc ccgtgtaacg
      241 tgctctaaga gcgttagcgt taaaaatggt gagagcacct tctcagatgc cgctaatgag
      301 caagcttcaa aggtgcttaa gccgggtaat gctctgcgaa agtactctgt gaaccctaac
      361 aagaagggca cttattgaga cataaaaagg ggcttcatag cccctgaaaa ttacagttga
      421 taagcgtcaa tatccagtag ctctttatca gcaatttcaa agattggtga cccatcattt
      481 agcggtaacg ggtttgtagg tatggtgatt tcagtgtcgt tctgtagcgt tacagttac
//
LOCUS       DAJTYZ010000407          537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000407 DAJTYZ010000000
VERSION     DAJTYZ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.412"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>537
                     /gene="ospC2"
                     /locus_tag="OO596_004495"
     CDS             <1..>537
                     /gene="ospC2"
                     /locus_tag="OO596_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6720106.1"
                     /translation="PIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDR
                     IINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALA
                     YSLGNAKSDMALYLLSKFNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEY
                     FINNGLVDVNKRFQKANS"
BASE COUNT          205 a           63 c           98 g          171 t
ORIGIN      
        1 tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt attgatttta
       61 taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac attgatcctg
      121 ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata ccaagaatgc
      181 taagcacaga taacttcaaa aagataagac tcagagatat atctttggag gatgccatta
      241 aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg gctcatcaag
      301 ctcttgctta ttctctaggt aatgcaaaat ctgatatggc actttattta ctatctaaat
      361 ttaattttac aaaacaagat gtcgcagaga tggagaaaat gaacaacaat atgtattgtg
      421 agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa gtgctagaat
      481 attttattaa taatggattg gttgatgtaa acaaaagatt tcaaaaagca aatagtg
//
LOCUS       DAJTYZ010000408          534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000408 DAJTYZ010000000
VERSION     DAJTYZ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.413"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..439
                     /locus_tag="OO596_004496"
     CDS             <1..439
                     /locus_tag="OO596_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6720107.1"
                     /translation="TDNKKTETDAIYDKVRSSYLLSCILKKNKNVGLILHAPSFVSVS
                     EKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFVHKIKLTEAIRKL
                     RRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          181 a           94 c           98 g          161 t
ORIGIN      
        1 cactgataat aaaaagacgg agacagatgc catttacgat aaagttcgga gttcttattt
       61 gctttcatgt attctgaaaa aaaacaaaaa tgttgggctt attcttcatg caccatcatt
      121 tgtttctgtg tctgaaaaaa tcgccagaat tgtgatggca aattactccc gtaactggag
      181 taattccgag ttggccagtg cggttttaat gagtgaatct tctttaaaaa gaagaatgta
      241 caaagaggtc ggttctataa gtacatttgt acataaaata aagcttacag aagctatcag
      301 gaaattacga aggacaaata ctcccatttc tgtcatatca tctgagttag gttacagttc
      361 accatcgtat ttttctaaag ttttctttaa atatttaaag acataccctc aaaacataag
      421 gaagaaaaat ggtcggtaat gtcaccaatt aggaggactt gcagagccgc ttttttacga
      481 tactcacccc gtaagtaagc gataattggt aacttaaacg cccatctgac atag
//
LOCUS       DAJTYZ010000409          521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000409 DAJTYZ010000000
VERSION     DAJTYZ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.414"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            109..441
                     /locus_tag="OO596_004497"
     CDS             109..441
                     /locus_tag="OO596_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6720108.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
BASE COUNT          150 a          135 c          123 g          113 t
ORIGIN      
        1 acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat cggtaaacag
       61 cacctttaga tgctgttttc gatacgcaat ttcaatcaag gagtcattat ggctggttgg
      121 tttgaactca gtaagagcag tgataatcag ttccggtttg tgctaaaagc gggcaatggt
      181 gagactatcc tcaccagcga gctgtataca tcaaaagcct ctgcggaaaa gggcatcgcg
      241 tcggtgcgta gcaacagccc gcaagaagaa cgctatgaga aaaaaacggc aagtaacggc
      301 aaattctatt tcaatctgaa agccgccaat catcaaatta tcggctccag ccagatgtac
      361 gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg cacaagccag
      421 acggtgaaag acaatacgta attacgatgc cgggcgtggg gtgtcggcgt ccggcgcttt
      481 accagatccc cccacagcgc tacaatgccc gcccttaaag t
//
LOCUS       DAJTYZ010000410          515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000410 DAJTYZ010000000
VERSION     DAJTYZ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.415"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>515
                     /locus_tag="OO596_004498"
     rRNA            <1..>515
                     /locus_tag="OO596_004498"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          127 a          124 c          156 g          108 t
ORIGIN      
        1 aatgtgcctt cgggaaccgt gagacaggtg ctgcatggct gtcgtcagct cgtgttgtga
       61 aatgttgggt taagtcccgc aacgagcgca acccttatcc tttgttgcca gcggtccggc
      121 cgggaactca aaggagactg ccagtgataa actggaggaa ggtggggatg acgtcaagtc
      181 atcatggccc ttacgaccag ggctacacac gtgctacaat ggcgcataca aagagaagcg
      241 acctcgcgag agcaagcgga cctcataaag tgcgtcgtag tccggattgg agtctgcaac
      301 tcgactccat gaagtcggaa tcgctagtaa tcgtggatca gaatgccacg gtgaatacgt
      361 tcccgggcct tgtacacacc gcccgtcaca ccatgggagt gggttgcaaa agaagtaggt
      421 agcttaacct tcgggagggc gcttaccact ttgtgattca tgactggggt gaagtcgtaa
      481 caaggtaacc gtaggggaac ctgcggttgg atcac
//
LOCUS       DAJTYZ010000411          510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000411 DAJTYZ010000000
VERSION     DAJTYZ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.416"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(265..366)
                     /locus_tag="OO596_004499"
                     /pseudo
     CDS             complement(265..366)
                     /locus_tag="OO596_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986316.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase"
BASE COUNT          123 a          142 c          118 g          127 t
ORIGIN      
        1 ccaggtatct tccagcgcag ccaacacaac ctcctgctgt ggtttcccag caaacagatc
       61 aaccgcctgt gggattggtg atttgtctcc ctgcaccgcc tcatacaaaa tttttgctcc
      121 aacacttata ttcagcgaac cagttgggcc gtcctgggtt gtttcgtagc cactggcgct
      181 gtaccactta tcaaatggca taggtacggc agccactacg gtacgcttca acatactggt
      241 cagccaaacg ttcaggatgg cagagcctgg ctgctgccag gttttaccat catcattaag
      301 caaattgatg ccatcccaac gtgttaatgt atccaccaac tgacggcgcg gatcgctctg
      361 tgtcaaacca gatgtcgctg cttgcagagt aggtaaaaaa agcctcaggt taagatcctg
      421 acgactggtt tggcgaataa catcccatgc ctgatcagca gttaagcgtg gcttttgctc
      481 aagcagtcgg tcgatctccg taacgcgatc
//
LOCUS       DAJTYZ010000412          503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000412 DAJTYZ010000000
VERSION     DAJTYZ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.417"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>503)
                     /gene="cmtA"
                     /locus_tag="OO596_004500"
     CDS             complement(<1..>503)
                     /gene="cmtA"
                     /locus_tag="OO596_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR6720109.1"
                     /translation="KECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQ
                     ASVNGKSIFFMVASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYV
                     LMKPLTIIAMIAGGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTV
                     ATLVSFA"
BASE COUNT          146 a          146 c          120 g           91 t
ORIGIN      
        1 tagcaaagga caccagggta gctaccgtaa caccggcaat tgtcgccagg aacgagcctt
       61 tcggcgtcag tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac
      121 cgtccagtaa gttaaacacc caggtgccag acataccgcc cgcaatcatg gcaataatgg
      181 tcagcggctt catcagcaca tacgggaaat acagttcgtg gatcccaccg aggaagtgaa
      241 taatcatcgc cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca
      301 gcagcagccc taagccaggc ccaggattgg acgccaccat aaagaagatg gatttaccgt
      361 taaccgaagc ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattga
      421 ggaaaagcac tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta
      481 ccagcgcctc aatacactct ttg
//
LOCUS       DAJTYZ010000413          503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000413 DAJTYZ010000000
VERSION     DAJTYZ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.418"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            39..>503
                     /locus_tag="OO596_004501"
     CDS             39..>503
                     /locus_tag="OO596_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6720110.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLP"
BASE COUNT          154 a          113 c           86 g          150 t
ORIGIN      
        1 tatcacattt tatcagaata tccttctgcg aggttattat gcttcctgta aataatcccc
       61 ccctatccac tggaaacgtc tctttttaca gaactacatc aatcgacaat gttcacaata
      121 attatctctc cgaatgggtt gaatggacta aaaacagcat ttccggagaa aacagggaaa
      181 ctgcttttac ccggctccaa ttatgtctgg agaacagtga aacatcgttg gacttatctt
      241 gtttaggtct cagatctcta ccacgattgc ctgacaatct tgatgaaatt aatgtaagca
      301 ataaccaact atcaatgctc cccgagctac caagggcatt gaaagagctg aatgcaagca
      361 gtaatcaatt atctgcactt cctgaattac cagtgtcgct ggaatatata aatgtgagtg
      421 ataaccattt gttcgcactt cctgaattac ctgcgtcact agaatatatt aatgtaagtg
      481 acaatcacct gtctgtactt ccg
//
LOCUS       DAJTYZ010000414          500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000414 DAJTYZ010000000
VERSION     DAJTYZ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.419"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..392
                     /locus_tag="OO596_004502"
     CDS             <1..392
                     /locus_tag="OO596_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="similar to the fimbrial usher protein PapC; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCR6720111.1"
                     /translation="NRIALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAI
                     MNITRSDGKNIPFAADIYDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSC
                     LAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            409..>500
                     /locus_tag="OO596_004503"
     CDS             409..>500
                     /locus_tag="OO596_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6720112.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFT"
BASE COUNT          171 a           84 c          112 g          133 t
ORIGIN      
        1 aaaaccgtat cgcgctggat atcaacgatc ttgagaacga tgttgaatta aaaagtacca
       61 gtgcagtagc tgtaccgcgt cagggttcag tcgtctttgc tgattttgaa accgtgcaag
      121 ggcaatcagc cattatgaac atcacacgaa gtgatggtaa aaatattcca tttgctgcag
      181 atatttatga tgagcaaggc aatgtcattg gtaatgttgg acagggtgga caagcatttg
      241 ttcgtggcat tgagcagcag ggaaatatca gcattaaatg gctcgaagaa agtaaacccg
      301 taagttgtct tgcgcattat caacaaagtt cagaagcaga aaaaatagcg caatctatta
      361 ttctgaatgg aattcggtgt cagattcagt aactacaagg aacgtcaaat gataaaaata
      421 acgccacata aaatagcgat actgatggga atattattat caccatcagt atttgcaacg
      481 gatattaatg tagagtttac
//
LOCUS       DAJTYZ010000415          498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000415 DAJTYZ010000000
VERSION     DAJTYZ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.420"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..415)
                     /locus_tag="OO596_004504"
     CDS             complement(<1..415)
                     /locus_tag="OO596_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6720113.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAI"
BASE COUNT          158 a          102 c           72 g          166 t
ORIGIN      
        1 taatagccgt atgtccttga cgagtcccca tagtagtgaa agcggaaagt gtgatttcta
       61 tttccacctt agataaatcc gctcgttccc cttcaggatt attcataaca aacttcatta
      121 tctcactgcc atcattattt ttgatataag atgaggactc aatatgagcc atcatatttc
      181 ttcccgcttc gttggagcgt ttgctaaaat ccacataacc tagagtaaat atttttacca
      241 aaaattccaa aaatgtctta ggatcaagga tatttcttgt gttatcctgc ccctcggcat
      301 tacgggcaaa attattcatt ccaacacgcc aggtatttaa aacttcactt ttttgtttaa
      361 aattgtcggc taatgtaatg tgttgagaat aattcaaatc tgatattttg ctcatgacga
      421 actccttgag ttaataagaa cgtctaagca ttacaaattt tactcacaaa aaaatatcaa
      481 aaaacgctct tttcacaa
//
LOCUS       DAJTYZ010000416          491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000416 DAJTYZ010000000
VERSION     DAJTYZ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.421"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(42..491)
                     /locus_tag="OO596_004505"
                     /pseudo
     CDS             complement(42..491)
                     /locus_tag="OO596_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          154 a           74 c           91 g          172 t
ORIGIN      
        1 aacaagacca gacacttata gaattagtga tgatttattc actaaaaagg ccatgaactg
       61 gaggaaaacg atctgtccag aaggtctatc ggttcctctc ttattgcctg attgtaataa
      121 tcgttattac agtaagtatt gtttatttca tctaaggtca tgtttttaac ttttatattc
      181 ctctggttta caacttcctc tgtcttgtaa gtgtgtatat tatatggggt atatgctatt
      241 tttaatagga tagtgaaaca attcgttatc cacatctatt ccgtcagagt gaacttcaaa
      301 tgcaccaggc gaaagaattg ccaggccagt attaagctta agtgttgggg aaaacaaatt
      361 aaataacatg agctgcatag tagggcaatt gttcttccca gaaacattta aaaaatttaa
      421 gtttttgctc tgttttatta tgggaaataa agttccaata tcggatgtat tattagtatc
      481 aatgtgggtt a
//
LOCUS       DAJTYZ010000417          487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000417 DAJTYZ010000000
VERSION     DAJTYZ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.422"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..388)
                     /locus_tag="OO596_004506"
     CDS             complement(<1..388)
                     /locus_tag="OO596_004506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720114.1"
                     /translation="MACDYRYKNRQYHCLSGSYMARSAKPRKRKPAPQRSKLLRYVVK
                     LHEDDFFDEEEAEVLRFDNFDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEI
                     ARCTEREFATIPAGISADGMYCPECGT"
BASE COUNT          129 a          118 c          112 g          128 t
ORIGIN      
        1 ccgtaccgca ttcagggcaa tacatgccgt cggcgctgat accggcagga attgtcgcaa
       61 attcccgctc cgtgcagcgg gcaatttccg gatacccttc gtcatcaaca cgtacaaacc
      121 agaagaccag ttttttgttt ccggcatcca caaagaacgg aatattcagg tctgcgcaac
      181 actcaacggc atcgtcaaaa ttatcaaagc gcagaacctc agcttcttct tcgtcaaaaa
      241 aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga ggtgcaggtt
      301 ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa tgatattgtc
      361 tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc gcctactcgg
      421 ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg cttggcagtt
      481 tggataa
//
LOCUS       DAJTYZ010000418          486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000418 DAJTYZ010000000
VERSION     DAJTYZ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.423"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>486)
                     /locus_tag="OO596_004507"
     CDS             complement(<1..>486)
                     /locus_tag="OO596_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6720115.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     K"
BASE COUNT          108 a          113 c          118 g          147 t
ORIGIN      
        1 ttcttaaaga tcccggtcgt tttcatctcc tcgtagtcgg gcatctcagg gttacgttcc
       61 ttcgttttcg catggagata tttgatccat tcatgctgac tgcgaccttc agtaaaggtt
      121 tgataaacgt ctggtcctaa gcgtttggcg acttcactca gcatccagta gatgggtttg
      181 cgttcaaatt ttgctgaggt tgcgggttgg gcgaggatca catagcccat attccctgca
      241 gattcatgag agataaggtc ttcttgctct gttggcatca ggtcgggcaa caggatatcg
      301 caatacttag ccgaggccgt catgaagtgg tcaatgccaa caatcatctc gcacttgctg
      361 tcatcctgaa gcacctcatg ggtgtgattg atgtcgccat gttgattgat caatgtgtta
      421 ctggcgtagc accataaaaa cttgatgggg acatccagtt tttcttttcc acgaacacca
      481 tcacgg
//
LOCUS       DAJTYZ010000419          482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000419 DAJTYZ010000000
VERSION     DAJTYZ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.424"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            43..303
                     /locus_tag="OO596_004508"
     CDS             43..303
                     /locus_tag="OO596_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720116.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            300..>482
                     /locus_tag="OO596_004509"
     CDS             300..>482
                     /locus_tag="OO596_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720117.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDA"
BASE COUNT          123 a          128 c          139 g           92 t
ORIGIN      
        1 tgatttttac ctgtcagaac attatcagat ggaacgtgca ggatggatca ggaaatgacg
       61 ttttcactca gctatgaaca actgacccgg tttgcggaaa aacgcatccg ggaatgcaat
      121 ctggacagcc acggcgtaac ttatctctgc gagtcagcca aagccggagc cgtgctgatc
      181 ttctggcatg aactggccat aaatggctat acctcaatga atgccataaa gagacaggag
      241 atcattgacg ccgaccatca gcgcctcaga aagctcatct ggccggagga tgactggaaa
      301 tgactcagag ccgccgaccg tcgccactgc agcggagagt gctgattgtg ctggctgccc
      361 ttgatgaaaa acggccagga cccgtgctga cgcgggacat agaacgggtg ctggagcaaa
      421 gcggggaagc cccggtctac gggccaaacc tgcgcgcctc ctgccgtcgt ctggaggatg
      481 cc
//
LOCUS       DAJTYZ010000420          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000420 DAJTYZ010000000
VERSION     DAJTYZ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.425"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            93..455
                     /locus_tag="OO596_004510"
     CDS             93..455
                     /locus_tag="OO596_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6720118.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKD"
BASE COUNT          115 a          112 c          139 g          110 t
ORIGIN      
        1 tagtcccggt ggtgatgtct atctcacacc gatgaacatg accacgaaac cctccgatgg
       61 cagtaaggcc ggtaagcaga aggataacgc caatgcagac gaaacaacgt cttgatgtac
      121 cgctgagtct gaaatctgtc agtgactccg gtgagtttga agggtatggc tccgtctttg
      181 gtgtaaagga cagccacgat gatgtggtga tgtccggggc atttgctgct tccctgcggg
      241 cgtggagtga cagaaaagcg ttacctgcgc tgctctggca gcaccgcatg gatgaaccca
      301 tcggtgttta caccgaaatg aaggaagacg atgtcgggct ttacgtcagg ggacggttgc
      361 ttattgatga tgatcccctc gcaaaacgtg cacatgcaca catgaaggcc ggttcgttaa
      421 ccggcctttc tattgggtac gtcctgaaag actgaggtag cctgagttta acggac
//
LOCUS       DAJTYZ010000421          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000421 DAJTYZ010000000
VERSION     DAJTYZ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.426"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            41..415
                     /locus_tag="OO596_004511"
     CDS             41..415
                     /locus_tag="OO596_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6720119.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            412..>476
                     /locus_tag="OO596_004512"
     CDS             412..>476
                     /locus_tag="OO596_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCR6720120.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASA"
BASE COUNT          130 a          106 c          127 g          113 t
ORIGIN      
        1 tcggaaagtt tgaaaaactg gtgcaggagg tgctgcgctg atgcgccatg agtttatttt
       61 accttatccg ccgacggtga atacttactg gcgacgtcgt gacaacacat attttgtatc
      121 aaaagccggt gagcgttatc gccgggatgt ggcgcttatt gtccgtcagc agcgactgaa
      181 attaagcctg tccggaagac tggcgataaa gattattgca gagccaccgg ataagcgccg
      241 tcgtgacctg gacaatatcc tgaaagcacc actggatgca ctgacacatg cgggagtgtt
      301 aatggacgat gagcagtttg atgaaatcaa tatcgttcgt ggtcagccag tatctggtgg
      361 acgtctgggg gtgaagattt accccataat gcatgaagag caggtcaaaa aatgaaactg
      421 gaagatttac cgaaatacta ctccccaaaa tccccttgcc tgaccgatgc atcggc
//
LOCUS       DAJTYZ010000422          467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000422 DAJTYZ010000000
VERSION     DAJTYZ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.428"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            116..>467
                     /gene="caiF"
                     /locus_tag="OO596_004513"
     CDS             116..>467
                     /gene="caiF"
                     /locus_tag="OO596_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6720121.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNR"
BASE COUNT          136 a           93 c          115 g          123 t
ORIGIN      
        1 cttatcgacg gtgaagttgc atactatcga tatatccaca attttaatat ggccttgttt
       61 aattgcttca aaacgagtca tagccagact tttaatttgt gaaactggag ttcgtatgtg
      121 tgaaggatat gttgaaaaac cactctactt gttaatcgcc gaatggatga tggctgaaaa
      181 tcggtgggtg atagcaagag agatctctat tcatttcgat attgaacaca gcaaggcggt
      241 taataccctg acttatattc tgtcggaagt cgcagaaata agctgcgcag ttaagatgat
      301 ccctaataag ctggaaggac ggggatgcca gtgccagcga ctggtaaaag tggtcgatat
      361 cgatgagcaa atttacgcgc gcctgcgcaa taacagccgg gataaattag tcggcgtaag
      421 aaagacgccg cgtattcctg ccgttccgct cacggaactt aaccgcg
//
LOCUS       DAJTYZ010000423          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000423 DAJTYZ010000000
VERSION     DAJTYZ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.429"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>464
                     /locus_tag="OO596_004514"
     CDS             <1..>464
                     /locus_tag="OO596_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HCR6720122.1"
                     /translation="QHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQ
                     RWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          112 a          115 c          137 g          100 t
ORIGIN      
        1 ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg
      121 ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg
      181 cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac
      241 cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg
      301 gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc
      361 tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa agtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DAJTYZ010000424          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000424 DAJTYZ010000000
VERSION     DAJTYZ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.430"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>461
                     /locus_tag="OO596_004515"
     CDS             <1..>461
                     /locus_tag="OO596_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6720123.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          134 a           94 c          143 g           90 t
ORIGIN      
        1 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg cggatctgta
       61 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaactgag
      121 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      181 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      241 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      301 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      361 acaacttgtg aaattaccga cgagaaggga aagggccttc ccgagagtgg taaaggagag
      421 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DAJTYZ010000425          457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000425 DAJTYZ010000000
VERSION     DAJTYZ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.431"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..309
                     /gene="gatA"
                     /locus_tag="OO596_004516"
     CDS             <1..309
                     /gene="gatA"
                     /locus_tag="OO596_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6720124.1"
                     /translation="TGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDND
                     VEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE
                     "
     gene            340..>457
                     /locus_tag="OO596_004517"
     CDS             340..>457
                     /locus_tag="OO596_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6720125.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQC"
BASE COUNT          137 a           89 c          104 g          127 t
ORIGIN      
        1 accgggataa tgcttgagca gcacgccatt gcaataccgc attgtgaggc gattcatgct
       61 aagtcgtcag ccatttatct gttaaggcca acaaataaag ttcattttca gcaagcggat
      121 gatgataacg acgtggaggt atcgttggtt attgcgttga ttgtggagaa tccgcagcag
      181 caattgaaac ttttacgctg tttatttggc aagttacaac agcccgatat cgtcgagaca
      241 ctaatcactc ttcctgaaac ccagttaaag gaatacttca caaagtatgt tttagattca
      301 gacgaataaa tccctctgta acaataaaaa ggactattta tgaaacgcaa gattattgtc
      361 gcttgcggag gcgcggttgc gacctctacg atggcggcgg aagaaattaa agagttgtgt
      421 cagaatcata gtattcctgt tgaattaatc cagtgtc
//
LOCUS       DAJTYZ010000426          453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000426 DAJTYZ010000000
VERSION     DAJTYZ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.432"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..350)
                     /locus_tag="OO596_004518"
     CDS             complement(<1..350)
                     /locus_tag="OO596_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720126.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILE"
BASE COUNT          150 a           83 c           85 g          135 t
ORIGIN      
        1 ttttcaagta tgcgaaaaac aaacttaacc gggtgatcag ggtaatccat ccagtcataa
       61 aatcctggga aatcattaaa ctgcacattt atggtgagtg cgttatcgtc tgaccactca
      121 cagtctcgta cggcagccat gagatgaatg agtttgtcgt catcgactaa atcatcacga
      181 caatcataag gataatcata atattgttta atcaaaacct tgaatatatc gacacaggaa
      241 actagggagc ttttgatatc ctctggtaac tgattgtttt taaccgtatt aagtatgctt
      301 tgttgcgtat atggcatcaa attattgtgc ggagctttta atgatatcat cgcgatctct
      361 cttaaataaa aataaacgtc gcagataata aagcgctgat taaaccaggc actatcagaa
      421 atcgctcata actaacgtga gaagtattgt gta
//
LOCUS       DAJTYZ010000427          446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000427 DAJTYZ010000000
VERSION     DAJTYZ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.433"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(15..>446)
                     /locus_tag="OO596_004519"
     CDS             complement(15..>446)
                     /locus_tag="OO596_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6720127.1"
                     /translation="KNVRMMVSGIVSYTRWPALSGPPKLCIFSSSRFSTALQENAATS
                     LPYLPVIIHTQQEAMISGCNGFYFGNESPTFQMELTEQYPSKALLLIAEQNTECIIGS
                     AFCLIIHNNDVRFAVNLDALSRSGVKVNPDVLMLARKKNDG"
BASE COUNT          128 a           93 c          103 g          122 t
ORIGIN      
        1 taagagaatt atcgttatcc atcatttttc ttccgtgcga gcattaagac atccgggttg
       61 acctttacac cgctacgcga taaggcatcc aggtttacgg caaatctgac gtcattattg
      121 tggatgatca gacaaaaggc gctaccaata atgcactcgg tattttgttc ggcaattaat
      181 aataacgcct ttgacggata ttgttcggtt aattccatct ggaatgtcgg tgactcgttg
      241 ccaaaataaa aaccattgca gcctgaaatc atcgcctctt gttgagtgtg aatgatgaca
      301 ggcagataag gcaaagatgt cgcggcattc tcttgtaggg cggtactaaa acgtgaagat
      361 gaaaagatac ataactttgg tggccctgat agcgcaggcc agcgggtgta actgacaata
      421 ccggaaacca tcatacgcac gttttt
//
LOCUS       DAJTYZ010000428          444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000428 DAJTYZ010000000
VERSION     DAJTYZ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.434"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..227
                     /locus_tag="OO596_004520"
     CDS             <1..227
                     /locus_tag="OO596_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6720128.1"
                     /translation="NIIFVDFQQQGERGLTNAPDEDPDDLSTGYYGSAYRSPENWTTA
                     LRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT          126 a           98 c          115 g          105 t
ORIGIN      
        1 ccaatattat tttcgtcgac ttccagcaac aaggtgaaag aggactgacg aacgcgcctg
       61 atgaagaccc ggacgattta agcacgggat attacggttc agcgtaccgg tcaccggaga
      121 actggacgac ggcactgcga agcagtcatt tcagcacggc agcccgtcgg gggattattt
      181 ctgacagatt tgtagaagca attttgcagt tttggcgcga aaggtgagcg gggagtttat
      241 tcatggggga tgtccttgtt cgttctcaga gtagacaaca aaaaacaaat aagatcaagg
      301 taatatctct tcacttgcgg gctcgaacag caggcaattg ccaatataat aatctggcac
      361 aagggtgaaa aacgcgcacc gcataaaccg ttgttattgc ttacagatta gccggatgcc
      421 ttaatagaca tccgcgcctt ttcg
//
LOCUS       DAJTYZ010000429          442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000429 DAJTYZ010000000
VERSION     DAJTYZ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.435"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>442
                     /locus_tag="OO596_004521"
     CDS             <1..>442
                     /locus_tag="OO596_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6720129.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAJTYZ010000430          436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000430 DAJTYZ010000000
VERSION     DAJTYZ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.436"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>436
                     /locus_tag="OO596_004522"
     CDS             <1..>436
                     /locus_tag="OO596_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6720130.1"
                     /translation="TLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEV"
BASE COUNT          127 a          113 c          122 g           74 t
ORIGIN      
        1 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
       61 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      121 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      181 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      241 atggataaag gttattactc actgggactg ttaaatgcct ggagcctggc gggagaacac
      301 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      361 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      421 ctgggaaatg aagtga
//
LOCUS       DAJTYZ010000431          424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000431 DAJTYZ010000000
VERSION     DAJTYZ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.437"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(34..306)
                     /locus_tag="OO596_004523"
     CDS             complement(34..306)
                     /locus_tag="OO596_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720131.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           93 a          101 c          117 g          113 t
ORIGIN      
        1 gtgaacaggc gtttcagcgg tcggggctga aaatcaccac cacttcgggt catccgcctt
       61 catctccgct tctgtttcgt ataaatcaaa acgacggcag gtatggcaga acgtgacgta
      121 ttcgtacggt gaatccgggt acattttgtg gaaggtttcc cagaaacaga ccgtgcagga
      181 gggatgaccg gtgataaagt ctgcaaatac ggactgataa gggtgattat tggctctggc
      241 gacggctttc agaacctctt tcaccattct ggtgtggact ttctggtgct ccaggttgtg
      301 tgacatggga actcattctg gatggttact ctgaaagctc atattctgcc acaccccgat
      361 ttgcagccgc caggctgccg tggttcaggg tcatttatca gccggtttac tcgccatggg
      421 tgaa
//
LOCUS       DAJTYZ010000432          423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000432 DAJTYZ010000000
VERSION     DAJTYZ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.438"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>423)
                     /locus_tag="OO596_004524"
     CDS             complement(<1..>423)
                     /locus_tag="OO596_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720132.1"
                     /translation="CPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFDIRLQIRDEGL
                     ILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQVLPEDVRLS
                     PHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSL"
BASE COUNT           81 a          141 c          129 g           72 t
ORIGIN      
        1 cagtgagcgc ccgaagccat ccaccacgac cgtcagtacc cggtatgcgg gcggctccgg
       61 ccagctgagt atccaccacg gcccctgaag gctgtttgtc gccaggtaca catgcggact
      121 caggcgaaca tcttccggca gcacctgcca gagcgcgctt agcggctgcg aactgtgctg
      181 cgccgccact ccgccccgcg ccagccagag tgactcgctg cggctgtagc ttatctcgcc
      241 gggaaacagc ggctcaaagt gaatgctcct gccgccgctg tcgttgagta tcaggccttc
      301 atcgcgtatc tgtaagcgga tatcgaaggg cgctttccag ccgggaccaa acacccccac
      361 cggcgcgggt gttctggtcc ggtagctgct gtaggcgcgg aaaagaataa acggcagcgg
      421 gca
//
LOCUS       DAJTYZ010000433          420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000433 DAJTYZ010000000
VERSION     DAJTYZ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.439"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            227..382
                     /locus_tag="OO596_004525"
                     /pseudo
     CDS             227..382
                     /locus_tag="OO596_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          125 a           87 c           97 g          111 t
ORIGIN      
        1 cacgcgttac gacaaaacgg cgagaaatta cctggcgatg gtgaaactgg gctgcatccg
       61 actcttttat caacgcttac gtaattaagg gacacagcct ggcactctgc gagtaaactt
      121 gctggtaaac tattattgac agttggtttt gttttgagac tgtaatttaa attgtgtaat
      181 tgcctgtttt tgatatgttc actccaacaa cggagacagg taaattatgg acgaaaagaa
      241 gcttaaagcc cttgcggctg aactggctaa aggcctcaaa accgaggccg acctcaatca
      301 gttttcccgt atgcagacga aattaactgt cgaaacggtg cttaatgcgg aactaactga
      361 ccatctcggg catgaaaaaa gctacatccg gtagtaaaca ttatcagtgg gttatgcgat
//
LOCUS       DAJTYZ010000434          415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000434 DAJTYZ010000000
VERSION     DAJTYZ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.440"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>415
                     /locus_tag="OO596_004526"
     CDS             <1..>415
                     /locus_tag="OO596_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6720133.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQ"
BASE COUNT          121 a           86 c          101 g          107 t
ORIGIN      
        1 tgggcttccg gacaaaatcg tagcaattac tctattggct acagtaacag tgcatcctgg
       61 ggcagctaca gtgtcagtgc ccagcgttca tggaatgaag acggcgacac tgacgatagc
      121 gtttatctca gtttcaccat tcctattgaa aaattacttg gcactgaaca acggacttca
      181 ggtttccaga gtattgatac tcaaatgagc agtgacttta agggtaataa ccaactcaac
      241 gtcagtagca gtggctatag tgataacgct cgcgtcagtt atagcgtaaa tactggctat
      301 acgatgaata aagccagcaa agatttgagt tatgttgggg gttatgccag ctatgaatca
      361 ccgtggggaa ccctggcagg ttcagtttct gcaaatagcg ataacagtcg tcaag
//
LOCUS       DAJTYZ010000435          407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000435 DAJTYZ010000000
VERSION     DAJTYZ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.441"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>407
                     /locus_tag="OO596_004527"
     CDS             <1..>407
                     /locus_tag="OO596_004527"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6720134.1"
                     /translation="QYIARVFNADRSYMVTNGTSTANKIVGMYSAPAGSTILIDRNCH
                     KSLTHLMMMSDVTPIYFRPTRNAYGILGGIPQSEFQHATIAKRVKETPNATWPVHAVI
                     TNSTYDGLLYNTDFIKKTLDVKSIHFDSAWVPYT"
BASE COUNT          107 a          119 c           85 g           96 t
ORIGIN      
        1 cagtatatcg ctcgcgtctt taacgcagac cgcagctaca tggtgaccaa cggtacttcc
       61 actgcgaaca aaattgttgg catgtactct gctccggcag gcagcaccat tctgattgac
      121 cgtaactgcc acaaatcgct gacccacctg atgatgatga gcgatgttac gccaatctat
      181 ttccgcccga cccgtaacgc ttacggtatt cttggtggta tcccacagag tgaattccag
      241 cacgctacca ttgctaagcg cgtgaaagaa acaccaaacg caacctggcc ggtacatgct
      301 gtaattacca actctaccta tgatggtctg ctgtacaaca ccgacttcat caagaaaaca
      361 ctggatgtga aatccatcca ctttgactcc gcgtgggtgc cttacac
//
LOCUS       DAJTYZ010000436          401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000436 DAJTYZ010000000
VERSION     DAJTYZ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.442"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..356)
                     /locus_tag="OO596_004528"
     CDS             complement(<1..356)
                     /locus_tag="OO596_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR6720135.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTA"
BASE COUNT          105 a          101 c          118 g           77 t
ORIGIN      
        1 tgcgctgttt tcgtgaacac gcggcggaca gcctcacttc ccaggcgctg gcgggcctga
       61 ataacggcac tgggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
      121 acaatctggt gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc
      181 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
      241 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      301 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      361 gcgtttccgg attcttacac agccactgga tcggtcaact g
//
LOCUS       DAJTYZ010000437          400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000437 DAJTYZ010000000
VERSION     DAJTYZ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.443"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>400)
                     /locus_tag="OO596_004529"
     CDS             complement(<1..>400)
                     /locus_tag="OO596_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6720136.1"
                     /translation="EQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAA
                     YAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQGKR
                     HNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT           83 a          110 c          135 g           72 t
ORIGIN      
        1 tgacccctgc ggggtataaa aagtcccgtc gcgcatcatg gcgaacagaa cgtcgcagcg
       61 tcgtctcgcc agggcgataa gcgcctgatt gtgtcgtttt ccctgactca ttttgcgggt
      121 gtagtaagcc ctggagagcg gatccctgag cgcggcgaag gccgacagga acaacgcccg
      181 tttgagagct ttattacccc gtcgcgaggg atgctcaccg cgtattgacg agccggatcg
      241 ccgagttacc ggcgcaaggc cagcataagc agcgagatgt gcggcagagg cgaaggcgcg
      301 gcaggcgacc tcggtgagga gtctggctgc ggtcctgaca ccgactccgg gcatactggt
      361 caggaccggg taaagagggt gagcaagaac tcgctgttct
//
LOCUS       DAJTYZ010000438          399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000438 DAJTYZ010000000
VERSION     DAJTYZ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.444"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..363)
                     /locus_tag="OO596_004530"
     CDS             complement(<1..363)
                     /locus_tag="OO596_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="phosphorylates aminoimidazole ribotide (AIR) which
                     forms 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate
                     (HMP-P); synthesis of HMP-P from AIR is complex and
                     requires the product of thiC gene at least; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCR6720137.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           85 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatacttt
//
LOCUS       DAJTYZ010000439          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000439 DAJTYZ010000000
VERSION     DAJTYZ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.445"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            29..244
                     /locus_tag="OO596_004531"
     CDS             29..244
                     /locus_tag="OO596_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6720138.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            249..>398
                     /locus_tag="OO596_004532"
     CDS             249..>398
                     /locus_tag="OO596_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HCR6720139.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAAL"
BASE COUNT          103 a           81 c          118 g           96 t
ORIGIN      
        1 tcagcttccg gataacggga gacggggtat gtaccagatg gaaaaaatca caacaggtgt
       61 gtcatacacc acgtcagcgg tgggaacggg ctactggttc ctgcagttgc tggacagggt
      121 ttccccgtct cagtgggcgg caataggcgt gctggggagt ctgctgtttg ggctgctgac
      181 atatctgact aacctgtatt tcaaaatcag agaggaccgt cgtaaggctg cacggggaga
      241 gtaattcaat gactcaaaac tatgaactga ttgtgaaagg gatccgcaat tttgagaata
      301 aagttacggt aactttagcg ttacgggaca aaaaacgctt tgacggtgaa atttttgacc
      361 tggacatctc gctggaccgt gttgaaggtg ccgcgctg
//
LOCUS       DAJTYZ010000440          396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000440 DAJTYZ010000000
VERSION     DAJTYZ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.446"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..336
                     /gene="istB"
                     /locus_tag="OO596_004533"
     CDS             <1..336
                     /gene="istB"
                     /locus_tag="OO596_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCR6720140.1"
                     /translation="QLSYARVLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSN
                     KGFADWGEMFGDHVLATAILDRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDD
                     EMVESGQHQ"
BASE COUNT          131 a           81 c          106 g           78 t
ORIGIN      
        1 caactgagtt atgcccgggt gttgatcctg gatgaaatag gctatctgcc gatgaacaga
       61 gaggaagcca gtctgttctt ccggctactg aaccgtcgat atgaaaaagc gagcatcata
      121 ctgacgtcaa acaaagggtt cgcagactgg ggagaaatgt tcggagatca cgtactggca
      181 acagcgatcc tggatcggct gctacatcac tcaaccacgc tgaatatcaa aggagagagt
      241 taccggttaa aagagaaacg taaagctgga gtgctgacca aaaacacaac gccaatcagt
      301 gatgatgaaa tggtggaaag cggacagcat cagtaacgaa agtatttagc gggcatgaaa
      361 atggcaaata acggtcaaac atcgtggcgt tgacat
//
LOCUS       DAJTYZ010000441          396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000441 DAJTYZ010000000
VERSION     DAJTYZ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.447"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            222..>396
                     /locus_tag="OO596_004534"
     CDS             222..>396
                     /locus_tag="OO596_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6720141.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLP"
BASE COUNT          141 a           70 c           82 g          103 t
ORIGIN      
        1 ttaaagattt taacgatcgc ttattgtaaa aataatagca aagaaaattg taagatacag
       61 accgtaaata aaaacatctt ccttggtcag gaaagaaatc ttgaaagcat atctggctac
      121 gacaaaatcg ttgtcaaata taataactgg atcaccaaag ataatggcga gtttgaactg
      181 gcggtccatt aattatcatt gtaataaacg aaggaatagt aatgaaaaag gtatttgcaa
      241 aatctcttct ggtcgcagcg atgttttctg ttgcaggctc cgcattggca gtacaaaagg
      301 atattactgt aacggccaac gttgatgccg ctctggatat gacacagacc gataacaccg
      361 cgttgccgaa agcagtagaa atgcaatatc tgccgg
//
LOCUS       DAJTYZ010000442          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000442 DAJTYZ010000000
VERSION     DAJTYZ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.448"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            49..>394
                     /locus_tag="OO596_004535"
     CDS             49..>394
                     /locus_tag="OO596_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6720142.1"
                     /translation="MRYNGLNNMFFSLCQINDNHSFTSSSHTKKTKSYNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKHFIDIALYANNK
                     CDDRITRRVYLSK"
BASE COUNT          159 a           56 c           58 g          121 t
ORIGIN      
        1 aatgcagaga atatgccttt tctttcttgt taattataag gatattttat gcgctataat
       61 ggtttaaata atatgttttt ctctctttgc cagattaacg ataaccactc tttcacaagt
      121 tcatcacata caaagaaaac aaaatcatat aattacagca aacatcataa aaacacgtta
      181 attgacaata aagccctctc tcttttcaag atggatgatc atgaaaaagt gataggtttg
      241 attcagaaaa tgaaaagaat ttatgatagt ttaccatcag gaaaaatcac gaaggaaacg
      301 gacaggaaaa tacataaaca ttttatagat atagctttat atgcaaataa taaatgtgac
      361 gatagaatta cgagaagagt ttaccttagt aaag
//
LOCUS       DAJTYZ010000443          392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000443 DAJTYZ010000000
VERSION     DAJTYZ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.449"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(95..>392)
                     /locus_tag="OO596_004536"
     CDS             complement(95..>392)
                     /locus_tag="OO596_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR6720143.1"
                     /translation="ALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLIS
                     IPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT          119 a          107 c           91 g           75 t
ORIGIN      
        1 cttagctcat gaaaggagcg caacaagatg atgaatcaac atataacaac atcttaaaaa
       61 aaggcctgac attacgccag gccttctgcg ttaattaatc acgcttttcc agcagggtcc
      121 ggtaaatcag accaccgata atgccgccga caattggcac cacccagaag aaccacagtt
      181 gttctaatgc ccagccgccc tggaagatag caaccgcggt gctgcgcgcc gggttaacag
      241 aagtgttagt caccggaata ctaattaagt gaatcagggt taaggccaga ccaatagcga
      301 tcggcgcaaa acctgccggc gcgaatttgt cggttgcgcc gtggatcacc aacaggaaac
      361 ctgcactcaa taccagttca actaccagcg cg
//
LOCUS       DAJTYZ010000444          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.450, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000444 DAJTYZ010000000
VERSION     DAJTYZ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.450"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>387
                     /locus_tag="OO596_004537"
     CDS             <1..>387
                     /locus_tag="OO596_004537"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6720144.1"
                     /translation="GTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTK
                     ALSLLRALTDFKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLP
                     DLMYRAFEVLPTMVMTPYAAFQKELH"
BASE COUNT           99 a           99 c           92 g           97 t
ORIGIN      
        1 acggcaccat gagcaacttt ggtattccgg ccagcatcgt ggcgaaatac ctcgacgaac
       61 atggcatcgt tgttgagaaa accggtccgt ataacctgct gttcctgttc agcatcggta
      121 tcgataagac caaagcactg agcctgctgc gtgctctgac tgacttcaaa cgtgcgttcg
      181 acctgaacct gcgtgtgaaa aacatgctgc cgtctctgta tcgtgaagat cctgaattct
      241 atgaaaacat gcgtattcag gaactggctc agaatatcca caaactgatt gttcaccaca
      301 atctgccgga tctgatgtat cgcgcatttg aagtgctgcc gactatggta atgactccgt
      361 atgctgcgtt ccagaaagag ctgcacg
//
LOCUS       DAJTYZ010000445          380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000445 DAJTYZ010000000
VERSION     DAJTYZ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.451"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..341
                     /gene="mhpC"
                     /locus_tag="OO596_004538"
     CDS             <1..341
                     /gene="mhpC"
                     /locus_tag="OO596_004538"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCR6720145.1"
                     /translation="FVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDF
                     GPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFN
                     QLVLNFLARP"
BASE COUNT           79 a          103 c          104 g           94 t
ORIGIN      
        1 tcttcgtttt cgataccagc gatttgaccg acgccctgtt tgaagcgcgc ctgaataata
       61 tgctgtcgcg ccgcgatcac ctggaaaact tcgttaagag cctggaagct aatccgaaac
      121 agttcccgga ttttggccca cgtctggcgg aaatcaaagc gcaaaccctg attgtctggg
      181 ggcgcaacga ccgctttgtg ccgatggatg cgggtctgcg tctgctgtcc ggcattgccg
      241 gttctgaact gcatatcttc cgcgactgtg gtcactgggc gcagtgggaa catgccgacg
      301 ctttcaatca attggtgctg aatttcctcg cacgccctta aggaatggtc atgacgaagc
      361 atactcttga gcaactggcg
//
LOCUS       DAJTYZ010000446          379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000446 DAJTYZ010000000
VERSION     DAJTYZ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.452"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(49..>379)
                     /locus_tag="OO596_004539"
     CDS             complement(49..>379)
                     /locus_tag="OO596_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6720146.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
BASE COUNT           79 a          126 c           77 g           97 t
ORIGIN      
        1 accagccaac cgcggcacgt tcctgcatac gacgtgccgc agcttctgtt attccacgat
       61 aaaattccta accgaattca aatccttcag actattcagc tcttccttca gctcccgctg
      121 acggcgataa atctcgtcat tgcgctcaac ctgtgcctga gccattgctg ccgccagttc
      181 ttccagttcc ggcatcgact gtttcacctg ctgattatcg gcatcacccc acgtcatcac
      241 gtcccgtaca atgtcggatt tcgcagccat taccaccgga taaaggcggc ccagtgagtt
      301 ggggccagca ttccagatac gaccgttcca ttcaaacgtg aacggcttcg cctcctgttc
      361 tgtgcgccat gcttcaatt
//
LOCUS       DAJTYZ010000447          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.453, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000447 DAJTYZ010000000
VERSION     DAJTYZ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.453"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(41..128)
                     /locus_tag="OO596_004540"
     tRNA            complement(41..128)
                     /locus_tag="OO596_004540"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:Ser,seq:tga)
BASE COUNT          109 a           94 c           74 g          101 t
ORIGIN      
        1 agaaaaaggg gttagcattt agctaacccc ttatcttatt tggcggaagc gcagagattc
       61 gaactctgga accctttcgg gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc
      121 acacttccgg aatgacgcgc actataaaca tcccgatgcg gcgtgtaaac ccctaatttg
      181 tttgtttgcc tgaaaaacag ccaaaagtgc attgatagcg tgaaataaca gcagattgat
      241 catttcatca ccatgaattc cttctctttt actcgtttag caaccggcta aacatcccca
      301 ccgcccggca aaaagaaaaa taggtccatt tttatcgcta aaagataaat ccacacagtt
      361 tgtattgttt tgtgcaaa
//
LOCUS       DAJTYZ010000448          377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000448 DAJTYZ010000000
VERSION     DAJTYZ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.454"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>377
                     /locus_tag="OO596_004541"
     CDS             <1..>377
                     /locus_tag="OO596_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6720147.1"
                     /translation="HLNAKNTTKLEYLNLSNNNLLPTNDIDQLISSKHLWHVLVNGIN
                     NDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTTQPPGLQNFTSINLDNNQLTHFDAT
                     NYDRLVKLSLNSNALESINFPQG"
BASE COUNT          145 a           81 c           50 g          101 t
ORIGIN      
        1 catttaaacg ccaaaaacac tacaaaactg gaatatttaa acttaagcaa taacaattta
       61 ctgccaacca atgacattga tcaactaata tcatcaaaac atctttggca tgtattagtt
      121 aacggcatca acaatgatcc acttgcacaa atgcagtact ggactgcagt aagaaatata
      181 attgatgaca ctaatgaagt gaccattgat ttatcaggac ttaatttaac cactcaacca
      241 ccagggctgc aaaacttcac atctatcaat cttgataata accaactcac acattttgat
      301 gcaaccaact acgatagact cgtaaagcta agtctgaata gtaatgctct tgagtcaata
      361 aattttcctc aaggcag
//
LOCUS       DAJTYZ010000449          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000449 DAJTYZ010000000
VERSION     DAJTYZ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.455"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            57..>374
                     /locus_tag="OO596_004542"
     CDS             57..>374
                     /locus_tag="OO596_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCR6720148.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKK"
BASE COUNT          109 a           83 c           96 g           86 t
ORIGIN      
        1 gaatattacc aggctggcga cgccgataac gaacaaaatc aataaaggac tatctcatga
       61 caacatttat tcagcttcat gtgttaaccg cttaccctgc tgccaacctt aaccgtgatg
      121 acaccggtgc gccaaaaact gtggtgctgg gtggagcaac gcgtctgcgc gtttcctcgc
      181 aaagtctgaa acgtgcgtgg cgcacttctg cactttttga acaggcactg gcgggccata
      241 ttggtattcg cagtgggcgt attgcgcgtg aggcggcaac tatcctgatt gagaaaggga
      301 ttgaagataa aaaagccatc gaatgggcgg caaaaattgc tgattatctt gggaaagcta
      361 aaaatgacaa aaaa
//
LOCUS       DAJTYZ010000450          369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000450 DAJTYZ010000000
VERSION     DAJTYZ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.456"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>369
                     /gene="frlA"
                     /locus_tag="OO596_004543"
     CDS             <1..>369
                     /gene="frlA"
                     /locus_tag="OO596_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR6720149.1"
                     /translation="YLKNAGSRPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPI
                     DPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENF
                     AAPTTTAIGATGSFMALLAG"
BASE COUNT           68 a          103 c           95 g          103 t
ORIGIN      
        1 tttatctgaa aaatgccgga agccgaccgc tggctttcct ctccggctgg gccagcttct
       61 gggccaacga tgcgccgtca ttgtcgatta tggcgctggc gattgtcagc aatcttggct
      121 ttttaacgcc tatcgatccg ttgctcggta aatttatcgc cgccggatta attatcgcct
      181 ttatgttgct acacctgcgc tccgttgaag gcggcgcagc gtttcagacg ctaattacca
      241 tcgccaaaat tatcccgttc actatcgtca ttggccttgg gatcttctgg tttaaagcgg
      301 agaattttgc cgcccctacc accactgcga ttggcgcaac gggcagcttt atggcgctgc
      361 tggcgggga
//
LOCUS       DAJTYZ010000451          368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000451 DAJTYZ010000000
VERSION     DAJTYZ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.457"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(80..292)
                     /locus_tag="OO596_004544"
     CDS             complement(80..292)
                     /locus_tag="OO596_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720150.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          104 a           81 c           65 g          118 t
ORIGIN      
        1 tgcgccatcg tagttctccg ctggcgcagt agaatgggtg ttcagcccgt tatgtagtat
       61 accagaatta atgccaatac taacaggatg ctctgactcg caattcatcc agcagtttat
      121 catttcccat aatgtcactt accctcatcg gtaaaaaaat tgcctttcga ccattacgat
      181 acatcattga ttttttggtt tcagggaagt aatccatttc gactataact gacaggtcat
      241 cacgacgtat gactgcgtat ttactactaa atagtttctt tattttttcc acgatgcctc
      301 caggtttata agtacaaacg gttatatcaa catagagaca aaaggtgatg ctgccaactt
      361 actgattt
//
LOCUS       DAJTYZ010000452          362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.458, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000452 DAJTYZ010000000
VERSION     DAJTYZ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.458"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          109 a           45 c           60 g          148 t
ORIGIN      
        1 attaaaaaat gatgtcattg cgcgataaat attcatctaa tcaatgtgat tatttcaaga
       61 tgtaagtttt ggtttctcgt ttgatttgtg aagtagatct ctatttttat ctgaactttt
      121 ttctatcgaa tcctattcat ggctcttgac tgaataaaaa taaatctatt agccaattta
      181 tattaacggc tgttatttat aagtgctcta taatttgaag gttcaattta aaccggctaa
      241 aaataacact ggaaattatt tgttggttat ttgttgggat ttgcttatgt atttgtagtg
      301 gtgttttcaa tactcggtag cattctcgca aatatcattt agtggtttac gtacgtaaaa
      361 aa
//
LOCUS       DAJTYZ010000453          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.459, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000453 DAJTYZ010000000
VERSION     DAJTYZ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.459"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            65..172
                     /locus_tag="OO596_004545"
     CDS             65..172
                     /locus_tag="OO596_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6720151.1"
                     /translation="MTLAELGMAFWHDLAALVIAGILASMIVNWLNKRK"
BASE COUNT           97 a           84 c          103 g           77 t
ORIGIN      
        1 attcttgaac ctgaacaact caagaatagc cgcgaaacgt tgtcattaca acacaggcgg
       61 ctatatgacg ctcgcagaac tgggcatggc cttctggcat gatttagcgg ctctggtcat
      121 tgctggcatt cttgccagta tgatcgtgaa ctggctgaac aagcggaagt aacgtgtcat
      181 gcgggcgtca ggctgccgta atggcaattt gcgcccggac caggccgcag gggtgaaact
      241 ctgcggcctt tttcgtaaga aaaatgcaac gattgcacgc ggataatatt ctgcggaatg
      301 cgcagcacag agtgaagacg tgacggattc cggaacagaa aaacagtcgt agaatcagcg
      361 g
//
LOCUS       DAJTYZ010000454          359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.460, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000454 DAJTYZ010000000
VERSION     DAJTYZ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.460"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>359)
                     /locus_tag="OO596_004546"
     CDS             complement(<1..>359)
                     /locus_tag="OO596_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6720152.1"
                     /translation="THFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRSVCE
                     HYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGNTHA
                     ERISAMLTSPAMNETHD"
BASE COUNT          106 a           78 c           85 g           90 t
ORIGIN      
        1 aatcatgagt ttcattcatt gccggagagg tcagcatggc gctgatacgc tctgcgtgcg
       61 tattgccgga acgtacggtc ttgaccagat tctcaatggg ttcaacatca gacaataacg
      121 tcgccgtagg gcacatgagc aattctactg cctgggaata ccaggagcgt gacaccatga
      181 aaacccagtg aatttcccga tattgtgaga ttaaagaata ataatgttca caaacagaac
      241 gtggatcttc actttcaccg gctaaatcag caatgactaa tccggaacga cgtaattgta
      301 ataatgtaag gtcctccgca gaggcgctgg atattatttc atattcaggg aagtgagtc
//
LOCUS       DAJTYZ010000455          355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.461, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000455 DAJTYZ010000000
VERSION     DAJTYZ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.461"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>355
                     /locus_tag="OO596_004547"
     CDS             <1..>355
                     /locus_tag="OO596_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6720153.1"
                     /translation="WCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFNSETV
                     QDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSPESN
                     GIAESFVKTIKRDYIS"
BASE COUNT           83 a           83 c          120 g           69 t
ORIGIN      
        1 atggtgctct gacgggttcg agttccgctg tgataacgga gaaaaactgc gagtcacgtt
       61 cgcgctggac tgctgtgacc gtgaggcact gcactgggcg gtcactacgg gcggcttcaa
      121 cagtgaaaca gtacaggacg tcatgctggg agcggtggaa cgccgcttcg gcaacgagct
      181 tccggcgtct ccagtagagt ggctgacgga taatggttca tgctaccggg ctaatgaaac
      241 acggcagttt gcccggatgt tggggcttga accgaagagc acggcggtgc ggagtccgga
      301 gagtaacggc atagcagaga gcttcgtgaa aacgataaag cgtgactaca tcagt
//
LOCUS       DAJTYZ010000456          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.462, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000456 DAJTYZ010000000
VERSION     DAJTYZ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.462"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..203)
                     /locus_tag="OO596_004548"
     CDS             complement(<1..203)
                     /locus_tag="OO596_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCR6720154.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPAGNDSNLLIVFYVQI"
     gene            complement(203..>353)
                     /locus_tag="OO596_004549"
     CDS             complement(203..>353)
                     /locus_tag="OO596_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HCR6720155.1"
                     /translation="SGVGGGIVAIVPHEMAIAVWSPELDDAGNSLAGIAVLEQLTKQL
                     GRSVY"
BASE COUNT          103 a           95 c           80 g           75 t
ORIGIN      
        1 attatctgaa cataaaacac tatcaataag ttggagtcat tacctgccgg agcaagacgt
       61 ttagcgacaa aatccgccgc atgttgttcg atgactggcg cgcctttctc caggcaatac
      121 tgacgctctt tcatgcccag acgaaagcga gagcgaaatt ttgaacggga taaacgcgcg
      181 aagagtggat cgagagactg cattaataaa ccgaacgccc taactgtttc gtcaattgtt
      241 caaggacggc gatacccgca agcgagttac ctgcatcatc cagttccgga ctccagactg
      301 cgatggccat ttcatgcgga acaatcgcca caataccgcc accaacgcca gat
//
LOCUS       DAJTYZ010000457          352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.464, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000457 DAJTYZ010000000
VERSION     DAJTYZ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.464"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..169)
                     /gene="rayT"
                     /locus_tag="OO596_004550"
     CDS             complement(<1..169)
                     /gene="rayT"
                     /locus_tag="OO596_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6720156.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVL"
     gene            complement(240..>352)
                     /locus_tag="OO596_004551"
     CDS             complement(240..>352)
                     /locus_tag="OO596_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6720157.1"
                     /translation="ILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT           97 a           83 c           81 g           91 t
ORIGIN      
        1 gcaaaacgac ccaggcgttg atttcaaaag gcctgtctcg cttaacttta ataatggcgt
       61 tacggagcat ctggtactgg gtggtcaaga gttggcttcg acgattgcgt aaattcaccg
      121 tgaaaaacca ggttcccccc ttgatgtaat aacgacgata ttcagacatg gcgactcctt
      181 tttctcaacg gtaagaagaa aaggaaaggt cggcaatgag gtgatggaac agggcaagat
      241 tacgaaagcc cgctcccctc aaggactgac gccagatagt ttctgtccat ggctgctttt
      301 cgcatcttac gtcttaaccc tgccttgaat accttatcat tcgtcaaaat at
//
LOCUS       DAJTYZ010000458          351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.465, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000458 DAJTYZ010000000
VERSION     DAJTYZ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.465"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(46..>351)
                     /locus_tag="OO596_004552"
     CDS             complement(46..>351)
                     /locus_tag="OO596_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6720158.1"
                     /translation="FRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRLY
                     FEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK"
BASE COUNT           88 a           92 c           74 g           97 t
ORIGIN      
        1 ggcgcaccgg ataaaaagcg ccatctaacg atggcgcgtc tggaactact ttttgttaac
       61 gtcaaacatg gtggcatcgg tcgccatgtc atcaataact tgtttaatat tttcgaaagc
      121 cattggcgta ctttcgttat tcaggtcttt accttcgccc tgacgaacga ctttgataac
      181 cggtttatta gtcgctgcat caatcagctc accttcaaaa tagagtcgag tatccatggt
      241 acggtggcct gtagccattt gcgtccccgc aaccactaat gcaacaggaa ccacttcata
      301 gaattgcagc ccttctttgc tggtatctac accggtaatg gccccacgga a
//
LOCUS       DAJTYZ010000459          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.467, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000459 DAJTYZ010000000
VERSION     DAJTYZ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.467"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>342
                     /locus_tag="OO596_004553"
     CDS             <1..>342
                     /locus_tag="OO596_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6720159.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEGV"
BASE COUNT           82 a          124 c           89 g           47 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaaggg gt
//
LOCUS       DAJTYZ010000460          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.468, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000460 DAJTYZ010000000
VERSION     DAJTYZ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.468"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(54..242)
                     /locus_tag="OO596_004554"
     CDS             complement(54..242)
                     /locus_tag="OO596_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720160.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
BASE COUNT           86 a           81 c          104 g           70 t
ORIGIN      
        1 gtgaacagac gtttcagcgg gcggggagta aaagcggaca acatgaggac ggattaaccc
       61 ccggtatcct gtagccgggt aagggctgct gtgtcgccac ggcgctccag gatggaggcc
      121 gcgaccatcg caggtggtgg gtccatgggt ttgagcatct tcatccagtc ccattcacgt
      181 tgtgagagct tctcagcacc aacagagagc agaacggtat ctatactttt gcgggtcaac
      241 ataagaggat gagtccttaa taaaacaggt cctcagagca tacatattct gcggagcgtg
      301 cggcaacaac aggacgaaca ggggcggttt tcaatttgca g
//
LOCUS       DAJTYZ010000461          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.469, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000461 DAJTYZ010000000
VERSION     DAJTYZ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.469"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           88 a           76 c           84 g           93 t
ORIGIN      
        1 gttttatcaa aaatataaga gacaaattct tctttagtca tatagatttc cttcttggtt
       61 attcgggata agaaggatac cacctcgcct gacgtggtta aaagcaggca cacaacgcga
      121 aatgacgcgg cggtaagtat ggcggagtta ttccttcccc gttgaggaca ccgggttgtc
      181 aggttgacca tacgcttaag tgacaaccac gccacaacac tccatgttga ttggtacctt
      241 tggcggcatc agtttcattg ctggctgatg tccgcccttt ttaaagtgaa ttttgtgatg
      301 cggtgaatgc ggctcagcgc acgcggcaca gttaaaaagc g
//
LOCUS       DAJTYZ010000462          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.470, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000462 DAJTYZ010000000
VERSION     DAJTYZ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.470"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..265)
                     /locus_tag="OO596_004555"
     CDS             complement(<1..265)
                     /locus_tag="OO596_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6720161.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDT"
BASE COUNT           88 a           84 c           66 g          102 t
ORIGIN      
        1 ttgtgtctgg ttcttccggc cactcaatat caggtgcagt tgatgtatca acacggttca
       61 gcaacacccg atacttcttc caggcttcca gcaacgagtt ttcttcctcc gttgcgatct
      121 ccagatctac agcatcctga agtggcgcaa tatgctcact gaattcctgg atgtagaact
      181 gtgtggtgac ggtcttccag ccattcggct cctgctgtat cgaagcatac caggctattt
      241 caatatcgct atgctgcggc agcatttaac cccttgtaat tcatcgccat aattgattta
      301 attcacaaat aaaactataa tatggtgaaa ttaatgaaaa
//
LOCUS       DAJTYZ010000463          336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.471, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000463 DAJTYZ010000000
VERSION     DAJTYZ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.471"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>336
                     /gene="ynaI"
                     /locus_tag="OO596_004556"
     CDS             <1..>336
                     /gene="ynaI"
                     /locus_tag="OO596_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6720162.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGI"
BASE COUNT           84 a           61 c           72 g          119 t
ORIGIN      
        1 tattaattat gttatagaaa actataaact caaatttatc accccagacg ttatcgattt
       61 tatctgtacg tccctgattg cggttattct gacgattaag ctatttttgc tgattaatca
      121 gtttgaaaaa cagcagatta aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg
      181 cattatcaaa atcaccatta ttgtggtgct tgttctgctt tatggcgaac atttcggcat
      241 gagcctttct ggcttgctga cctttggtgg tattggtggt ctggctgtcg gtatggccgg
      301 taaagatatt ctgagtaatt tcttttccgg gatcat
//
LOCUS       DAJTYZ010000464          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.473, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000464 DAJTYZ010000000
VERSION     DAJTYZ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.473"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           96 a           70 c           48 g          113 t
ORIGIN      
        1 gaatatctca atttttaaac ataaaatgcg aattatttag tacaaaaagt gaacttctga
       61 tgatctttcc ctggcctgcc accatctctt cacaagctta tgcttattac atgttacatg
      121 caaaacgtcc cgttttgcat ctttgtgcct acggcacacc gtgatctttc cctgatgagg
      181 gatatattaa ctattgaaca ttagcgcgaa ttatttagtg caaaaaatca gcatcacgtg
      241 atgcttttcc tgctattaat aacctcctct atttaactat atttttcatt gatgcaattc
      301 gtagatttga agaagttcaa ctgccac
//
LOCUS       DAJTYZ010000465          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.474, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000465 DAJTYZ010000000
VERSION     DAJTYZ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.474"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>325
                     /locus_tag="OO596_004557"
     CDS             <1..>325
                     /locus_tag="OO596_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR6720163.1"
                     /translation="TEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVY
                     HQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTL
                     RINGK"
BASE COUNT           93 a           62 c           79 g           91 t
ORIGIN      
        1 atacagagga gattcaacgc gccgtgattc tgaaaatgga aatgatgcca tttttagact
       61 cggtgggact ggtacttgat gataataaat attatctctt ttcgcggagg gcgaatgata
      121 aaatcgttgt ttatcatcag gaacaagtaa atggaccgct tgtcgacgag tcagggcggg
      181 ttatttttgc cgattttaac ccatcgaaac gaccgtggtc ggtggcttca gatgactcta
      241 acaacagctg gaatccggca tacaattgct ttgatcgtcc gggtaaaaaa tgtatctctt
      301 ttacgctacg tatcaacggc aaaga
//
LOCUS       DAJTYZ010000466          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.475, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000466 DAJTYZ010000000
VERSION     DAJTYZ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.475"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>322
                     /locus_tag="OO596_004558"
     CDS             <1..>322
                     /locus_tag="OO596_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6720164.1"
                     /translation="VQIRQNQPHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVN
                     GHAHLLYALNIAVRTAPDSSVKALKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQ
                     VMEWR"
BASE COUNT           73 a           84 c           95 g           70 t
ORIGIN      
        1 atgtccagat ccggcagaac cagcctcatg cgcagttctg gctggttttt gacgttgatc
       61 gcgagggggc agcgattgac tggagcgacc ggaacgctcc cgcgccgaac atcaccgtaa
      121 aaaatcctgt gaacggtcat gctcacctgc tctacgcgct caatatcgcc gtgagaaccg
      181 cgccggacag ctcggttaag gcgctgaaat acgccgctgc gattgaacgt tcgctgtgtg
      241 agaaactttg tgctgatgtg aactacagcg gactgatttg taaaaatccg ttccacctgg
      301 aatggcaggt gatggagtgg cg
//
LOCUS       DAJTYZ010000467          319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.476, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000467 DAJTYZ010000000
VERSION     DAJTYZ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.476"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(20..269)
                     /locus_tag="OO596_004559"
                     /pseudo
     CDS             complement(20..269)
                     /locus_tag="OO596_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          107 a           62 c           54 g           96 t
ORIGIN      
        1 cagggggcca gtctaagttc taagagagca cggattccct gtcattaata atgatatccg
       61 ttagtgtagt cttatgcatt tcaatgtact gcaatatttg cagatagaga tcagaaagaa
      121 tcacactggt ttcatctttt aaacgcaagc gtaaagcctg atcaataata tgactatcaa
      181 catacccatg cgtcatgaac acctccctct cgagattttt aaacaaagca cattgaaaaa
      241 ataaatctct ctgggattga attattcatg tcagcctcgt agaaatatta acctaataaa
      301 attatgctgt gtatagtct
//
LOCUS       DAJTYZ010000468          314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.477, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000468 DAJTYZ010000000
VERSION     DAJTYZ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.477"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>314
                     /locus_tag="OO596_004560"
     CDS             <1..>314
                     /locus_tag="OO596_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6720165.1"
                     /translation="YHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMA
                     HQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANY
                     KV"
BASE COUNT          130 a           42 c           52 g           90 t
ORIGIN      
        1 agtatcatat accaagaatg ctaagtacag ataacttcaa aaaaataaaa ctcagagaca
       61 tatctttgga ggatgcgatt aaagcatcta attatgaaga gattaacaat aaagtgactg
      121 ataaaaaaat ggctcatcag gcccttgctt attctttagg caataaaaaa gctgatatag
      181 cactttattt actatctaaa tttaatttta caaaacaaga tgtcgcagag atggagaaaa
      241 tgaacaacaa tagatattgt aatctgtatg atgttgaata cttacttagt aaagatggtg
      301 ccaactataa agtg
//
LOCUS       DAJTYZ010000469          314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.478, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000469 DAJTYZ010000000
VERSION     DAJTYZ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.478"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          123 a           66 c           30 g           95 t
ORIGIN      
        1 tacaccagca tggacatcat tgtactatgc cccgtacatg ccccatcaca tcctcaacat
       61 caaagtatta gagccttgca aaccattatg cgtctcagtt tgtctcatct atcttacgca
      121 aaaaaaaata gaactaatat caagcggcgc tatattgcgt attaaataaa accacctcaa
      181 ttacattaat aaatctaaac aaacatcatg caaataattt ccatggttat ttctaggcta
      241 tttaaattaa ataaataaat tatgagtcaa tcataaataa caacctttaa tataatttaa
      301 ctaccaaatt tata
//
LOCUS       DAJTYZ010000470          313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.479, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000470 DAJTYZ010000000
VERSION     DAJTYZ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.479"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>313
                     /locus_tag="OO596_004561"
     CDS             <1..>313
                     /locus_tag="OO596_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6720166.1"
                     /translation="DKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNKQN
                     DSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLSRK
                     LQ"
BASE COUNT          114 a           58 c           80 g           61 t
ORIGIN      
        1 tgataagcaa atggtggcgg cgcagaaagc agcggatgca tattggggaa aagatgcaaa
       61 cggtaagcaa atgacccgcg aagaagcctt taaaaaaatt caccaacagc gtgatgagtt
      121 taataaacag aacgatagcg aggcgttcgc ggttaaatat gacaaagagg tttatcaacc
      181 ggcgattgcg gcatgtcata aacagagtga agagtgttat gaagtgccga ttcagcagaa
      241 acgagatttc gatatcaacg aacaacggcg acagactttt ctgcaatcac aaaaactcag
      301 ccgtaaattg cag
//
LOCUS       DAJTYZ010000471          306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.480, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000471 DAJTYZ010000000
VERSION     DAJTYZ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.480"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..147
                     /locus_tag="OO596_004562"
     CDS             <1..147
                     /locus_tag="OO596_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6720167.1"
                     /translation="CKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQSDAWI
                     MRLA"
     gene            168..>306
                     /locus_tag="OO596_004563"
     CDS             168..>306
                     /locus_tag="OO596_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCR6720168.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DI"
BASE COUNT           68 a           76 c           71 g           91 t
ORIGIN      
        1 tgtaaatctg accccgattt atgtatgcaa ctcgatgcct gggatgctga aaccagtatc
       61 cctgccctgc taaatggcga acactctgtc ctttaccgta cccgttacga tcaacaatct
      121 gatgcctgga ttatgcgtct tgcctgatcc aaaaagaacc cgtcgggatg gcgggttatt
      181 tgtcctggtt attcccccgt tgtaaaatct ctcctaaact taacggtacg gcaccacact
      241 tcggggatga aatgttcgcg ctggtacttt ttgtttgcta cctggatggc ggttgtgaag
      301 atattg
//
LOCUS       DAJTYZ010000472          305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.481, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000472 DAJTYZ010000000
VERSION     DAJTYZ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.481"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..104
                     /locus_tag="OO596_004564"
     CDS             <1..104
                     /locus_tag="OO596_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6720169.1"
                     /translation="GPVTEDVPASVLQLHPSLIVIADKAAAAELALG"
     gene            complement(166..>305)
                     /locus_tag="OO596_004565"
     CDS             complement(166..>305)
                     /locus_tag="OO596_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR6720170.1"
                     /translation="LEEDCENFGIRNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDF
                     S"
BASE COUNT           86 a           77 c           64 g           78 t
ORIGIN      
        1 aaggtccggt taccgaagat gttccagctt ccgtgttgca gctgcatcct tcgctgatag
       61 taatcgctga taaagccgct gccgcagaat tagcgctcgg ttaagctgaa gaaataaggc
      121 cggatgtatg aattacgctc cggcctgttc tcattattta aataactacg aaaaatcacc
      181 ccacagtctc agcttgccgc agtggagaca gcggaagaga taaccctgac aatcaccacc
      241 cttttgcagg catttagcta gatcagaatt tctaataccg aaattctcac aatcttcttc
      301 aaggt
//
LOCUS       DAJTYZ010000473          305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.482, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000473 DAJTYZ010000000
VERSION     DAJTYZ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.482"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>305)
                     /locus_tag="OO596_004566"
     CDS             complement(<1..>305)
                     /locus_tag="OO596_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6720171.1"
                     /translation="LANRYDWQLFIEVTGPGGSGKSVMAEICTMLAGKANTVSASMKA
                     LEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGGDKVAIDPKHKAPYSTRIPAV"
BASE COUNT           61 a          100 c           67 g           77 t
ORIGIN      
        1 tactgccgga atacgcgttg agtagggggc tttgtgtttc gggtcaattg ccaccttgtc
       61 accgcctgta atggccttaa tccctgcccc atcaccagcg tagcgggtca tatccggcat
      121 gataatcagc gaaaagccaa ccactaacgc gcgttccctt gcatcttcca gcgccttcat
      181 gcttgccgat actgtgttgg ccttacccgc cagcatggtg caaatctccg ccatcacgct
      241 tttaccactt ccccctggac ctgttacctc aatgaataac tgccagtcgt accggttcgc
      301 cagca
//
LOCUS       DAJTYZ010000474          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.483, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000474 DAJTYZ010000000
VERSION     DAJTYZ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.483"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>304)
                     /locus_tag="OO596_004567"
     CDS             complement(<1..>304)
                     /locus_tag="OO596_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720172.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKR"
BASE COUNT           69 a           84 c           79 g           72 t
ORIGIN      
        1 tacgcttcag tacagcatgc catggtcccc agagggagaa ccagtccgta aattcattct
       61 cttcacggct tctgaccatg gcttcggcag tgcggaggtc atttgctgtc actcccgaca
      121 cgccatagaa acgcatttcc ttcacggcag tggagagctg aagtttctct gcgagcatgg
      181 tctggaaggc caggtagact tctatctcat ccacaaaatg gagagttctg actttatccc
      241 gggcaatgtc ctccagaatt tcgaggcgga acatttccct gcccagggag agcagagcgc
      301 cggt
//
LOCUS       DAJTYZ010000475          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.484, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000475 DAJTYZ010000000
VERSION     DAJTYZ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.484"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            143..303
                     /locus_tag="OO596_004568"
                     /pseudo
     CDS             143..303
                     /locus_tag="OO596_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT           74 a           76 c           90 g           63 t
ORIGIN      
        1 ggattccgcc tcacgaccgc accaggtgat ggtttgtacg ttaactatgg gctgaaagcg
       61 ctgaacatcc atggtgggca aaagctgacg ttagccgaac atggcggagc ctatggcgca
      121 acggccgata tgtcggcaaa atagccatcg cttacacgac cgtctgggtg agccgcagta
      181 tacagattca ctgcattctc agggatcggc aagcagtatg tggatgcgtc atgtcggggg
      241 gcacgaacgt ttcaggaccg gtgacggcca gctaaatact caggctaacc gctatgtatt
      301 gca
//
LOCUS       DAJTYZ010000476          301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.denovo.485, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000476 DAJTYZ010000000
VERSION     DAJTYZ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.denovo.485"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>301
                     /locus_tag="OO596_004569"
     CDS             <1..>301
                     /locus_tag="OO596_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6720173.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT           67 a           91 c          101 g           42 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 t
//
LOCUS       DAJTYZ010000477         5016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.guided.208, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000477 DAJTYZ010000000
VERSION     DAJTYZ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.guided.208"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..652
                     /locus_tag="OO596_004570"
                     /pseudo
     CDS             1..652
                     /locus_tag="OO596_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /locus_tag="OO596_004571"
     CDS             649..1263
                     /locus_tag="OO596_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR6720174.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="OO596_004572"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="OO596_004572"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6720175.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="OO596_004573"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="OO596_004573"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6720176.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="OO596_004574"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="OO596_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6720177.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..>5016
                     /locus_tag="OO596_004575"
     CDS             4890..>5016
                     /locus_tag="OO596_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crl family RNA polymerase assembly factor"
                     /protein_id="HCR6720178.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCV"
BASE COUNT         1206 a         1243 c         1333 g         1234 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtca
//
LOCUS       DAJTYZ010000478         4475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.guided.219, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000478 DAJTYZ010000000
VERSION     DAJTYZ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.guided.219"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1430
                     /gene="frdA"
                     /locus_tag="OO596_004576"
     CDS             <1..1430
                     /gene="frdA"
                     /locus_tag="OO596_004576"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6720179.1"
                     /translation="AADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLDILVDDGHVRGL
                     VAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMALSHGVPLRDMEF
                     VQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLGEPKNKYMELGP
                     RDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICELAKAYVGVDPV
                     KEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRLGSNSLAELVVF
                     GRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMGLAME
                     EGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIELGHGLNVAECM
                     AHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPA
                     KRVYGGEADAADKAEAANKKEKANG"
     gene            1423..2157
                     /gene="frdB"
                     /locus_tag="OO596_004577"
     CDS             1423..2157
                     /gene="frdB"
                     /locus_tag="OO596_004577"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6720180.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2168..2563
                     /gene="frdC"
                     /locus_tag="OO596_004578"
     CDS             2168..2563
                     /gene="frdC"
                     /locus_tag="OO596_004578"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6720181.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            2574..2933
                     /gene="frdD"
                     /locus_tag="OO596_004579"
     CDS             2574..2933
                     /gene="frdD"
                     /locus_tag="OO596_004579"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6720182.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            2996..4129
                     /locus_tag="OO596_004580"
     CDS             2996..4129
                     /locus_tag="OO596_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6720183.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4218..>4475
                     /gene="blc"
                     /locus_tag="OO596_004581"
     CDS             4218..>4475
                     /gene="blc"
                     /locus_tag="OO596_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6720184.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMW"
BASE COUNT         1085 a         1168 c         1202 g         1020 t
ORIGIN      
        1 tcgccgccga taagaccggc ttccatatgc tgcacacgct gttccagacc tctctgcaat
       61 tcccgcagat ccagcgtttt gacgaacatt tcgtgctgga tattctggtt gatgatggtc
      121 atgttcgcgg cctggtagca atgaacatga tggaaggcac gctggtgcag atccgtgcta
      181 acgcggtcgt tatggctact ggcggtgcgg gtcgcgttta tcgttacaac accaacggcg
      241 gcatcgttac cggtgacggt atgggtatgg cgctaagcca cggcgttccg ctgcgtgaca
      301 tggaattcgt tcagtatcac ccaaccggtc tgccaggttc cggtatcctg atgaccgaag
      361 gctgccgtgg tgaaggcggt attctggtca acaaaaatgg ctaccgttat ctgcaagatt
      421 acggcatggg cccggaaact ccgctgggcg agccgaaaaa caaatatatg gaactgggtc
      481 cacgcgacaa agtttctcag gccttctggc acgaatggcg taaaggcaac accatctcca
      541 cgccgcgtgg cgatgtggtt tatctcgact tgcgtcacct cggcgagaaa aaactgcatg
      601 aacgtctgcc gttcatctgc gaactggcga aagcgtacgt tggcgtcgat ccggttaaag
      661 aaccgattcc ggtacgtccg accgcacact acaccatggg cggtatcgaa accgatcaga
      721 actgtgaaac ccgcattaaa ggtctgttcg ccgtgggtga atgttcctct gttggtctgc
      781 acggtgcaaa ccgtctgggc tccaactccc tggcggaact ggtggtcttc ggccgtctgg
      841 ccggtgaaca agcgacagag cgtgcagcaa ctgccggtaa tggcaacgaa gcggcaattg
      901 aagcgcaggc agctggcgtt gaacaacgtc tgaaagatct ggttaaccag gatggcggcg
      961 aaaactgggc gaagatccgc gacgaaatgg gcctggcaat ggaagaaggt tgcggtatct
     1021 accgtacgcc ggaactgatg cagaaaacca tcgacaagct ggcagagctg caggaatgct
     1081 tcaagcgcgt gcgcatcacc gacacctcca gcgtgttcaa caccgacctg ctctacacca
     1141 ttgaactggg ccacggtctg aacgttgctg aatgtatggc gcactccgca atggcacgta
     1201 aagagtcccg cggcgcacac cagcgtctgg acgaaggttg caccgagcgt gacgacgtca
     1261 acttcctcaa acacaccctc gccttccgcg atgctgatgg cacgactcgc ctagagtaca
     1321 gcgacgtgaa gattactacg ctgccgccag ctaaacgcgt ttacggtggc gaagcggatg
     1381 cagccgataa ggcggaagca gccaataaga aggagaaggc gaatggctga gatgaaaaac
     1441 ctgaaaattg aggtggtgcg ctataacccg gaagtcgata ccgcaccgca tagcgcattc
     1501 tatgaagtgc cttatgacgc aactacctca ttactggatg cgctggtcta catcaaagac
     1561 aacctggcac cggacctgag ttaccgctgg tcctgccgta tggcgatttg tggttcctgc
     1621 ggcatgatgg ttaacaacgt gccaaaactg gcatgtaaaa ccttcctgcg tgattacacc
     1681 gacggtatga aggttgaagc gttagctaac ttcccgattg aacgcgatct ggtggtcgat
     1741 atgacccact tcatcgaaag tctggaagcg atcaaaccgt acatcatcgg caactcccgc
     1801 accgcggatc agggtactaa catccagacc ccggcgcaga tggcaaagta tcaccagttc
     1861 tccggttgca tcaactgtgg tctgtgctac gccgcgtgcc cgcagtttgg cctgaaccca
     1921 gagttcatcg gtccggctgc cattacgctg gcgcatcgtt ataacgaaga tagccgcgac
     1981 cacggtaaga aggagcgtat ggcgcagttg aacagccaga acggcgtatg gagctgtact
     2041 ttcgtgggct attgctccga agtctgcccg aaacacgtcg atccggctgc ggccattcag
     2101 cagggcaaag tagaaagttc gaaagacttt cttatcgcga ccctgaaacc acgctaagga
     2161 gtgcaacatg acgactaaac gtaaaccgta tgtacggcca atgacgtcca cctggtggaa
     2221 aaaattgccg ttttatcgct tttacatgct gcgcgaaggc acggcggttc cggctgtgtg
     2281 gttcagcatt gaactgattt tcgggctgtt tgccctgaaa aatggcccgg aagcctgggc
     2341 gggattcgtc gactttttac aaaacccggt tatcgtgatc attaacctga tcactctggc
     2401 ggcagctctg ctgcacacca aaacctggtt tgaactggca ccgaaagcgg ccaatatcat
     2461 tgtaaaagac gaaaaaatgg gaccagagcc aattatcaaa agtctctggg cggtaactgt
     2521 ggttgccacc atcgtaatcc tgtttgttgc cctgtactgg taaggagcct gagatgatta
     2581 atccaaatcc aaagcgttct gacgaaccgg tattctgggg cctcttcggg gccggtggta
     2641 tgtggagcgc aattattgcg ccggtgatga tcctgctggt gggtattctg ctgccactgg
     2701 ggctgtttcc gggtgatgcg ctgagctacg agcgcgttct ggcgttcgcg cagagcttca
     2761 ttggtcgcgt attcctgttc ctgatgatcg ttctgccgct gtggtgtggt ttacaccgta
     2821 tgcaccacgc gatgcacgat ctgaaaattc acgtgcctgc gggcaaatgg gttttctacg
     2881 gtctggctgc tatcctgaca gttgtcacgc tgattggtat cgttacaatc taacgtatcg
     2941 ccaatgtaaa tccggcccgc ctatggcggg ccgttttgta tggaaaccag accttatgtt
     3001 caaaacgacg ctctgcgcct tattaattac cgcctcttgc tccacatttg ctgcccctca
     3061 acaaatcaac gatattgtgc atcgcacaat taccccgctt atagagcaac aaaagatccc
     3121 cggtatggcg gtggcggtaa tttatcaggg taaaccttat tactttacct ggggctatgc
     3181 ggacatcgcc aaaaagcagc ccgtcacaca gcaaacgttg tttgagttag gttcggtcag
     3241 caaaacattt acgggcgtgc ttggtggcga cgctattgct cgaggggaaa tcaagttaag
     3301 cgatcccgca acaaaatact ggcctgaact tactgctaaa cagtggaatg ggatcacact
     3361 attacatctt gcgacctaca ccgctggcgg cctgccattg caggtgccgg atgaagtgaa
     3421 atcctcaagc gacttgctgc gcttctatca aaactggcag cctgcatggg ctccaggaac
     3481 acaacgtctg tatgccaact ccagtatcgg tttgttcggc gcactggctg tgaagccgtc
     3541 tggtttgagt tttgagcagg cgatgcaaac tcgtgtcttc cagccactca aactcaacca
     3601 tacgtggatt aatgtacctc ccccagaaga aaagaattac gcctggggat atcgcgaagg
     3661 taaggcagtg catgtttcgc caggggcgtt agatgctgaa gcttatggtg tgaagtcgac
     3721 cattgaagat atggcccgct gggtgcaaag caatttaacc ccccttgata tcaatgagaa
     3781 aacgcttcaa caagggatac aactggcaca atctcgctac tggcaaaccg gcgatatgta
     3841 tcagggcctg ggctgggaaa tgctggactg gccggtcaat cctgacagca tcattaacgg
     3901 cagtgacaat aaaattgcac tggcagcacg cccggtaaaa gcgattaagc ccccaactcc
     3961 tgcagtacgc gcatcatggg tacataaaac gggggcgacc ggcggatttg gtagctatgt
     4021 cgcgtttatt ccagaaaaag agctgggtat cgtgatgctg gctaacaaaa actatcccaa
     4081 tccagcgaga gtcgccgccg cctgtcagat tctcaacgct ctacagtaaa atttcatcgg
     4141 gtccgaattt tcggaccttt tctgcgcttt tccttgctgt catctacact tagaaaaaaa
     4201 ccagtaagga aacatttatg cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc
     4261 tggtcgttgc ctgcagttct cctacgccgc cgcgtggcgt gacagtagtg aataatttcg
     4321 acgctaaacg ctatctcggt acctggtatg agattgcccg ttttgatcac cgttttgaac
     4381 gtggactgga aaaagtcacc gcaacataca gtctgcgtga tgacggcggc ctgaatgtca
     4441 tcaataaagg ctataaccct gacagagaaa tgtgg
//
LOCUS       DAJTYZ010000479         3864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.guided.243, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000479 DAJTYZ010000000
VERSION     DAJTYZ010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.guided.243"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="OO596_004582"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="OO596_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6720185.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1872..2675
                     /locus_tag="OO596_004583"
                     /note="aph(3'')-Ib"
     CDS             1872..2675
                     /locus_tag="OO596_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6720186.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2675..3511
                     /locus_tag="OO596_004584"
     CDS             2675..3511
                     /locus_tag="OO596_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6720187.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          832 a         1086 c         1065 g          881 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1861 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
     1921 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     1981 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     2041 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     2101 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     2161 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     2221 ttgatcaatg tccgtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     2281 tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc
     2341 tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
     2401 atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
     2461 ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
     2521 tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
     2581 ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
     2641 tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
     2701 tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
     2761 agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
     2821 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     2881 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
     2941 tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
     3001 gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
     3061 ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
     3121 cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
     3181 acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
     3241 tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     3301 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     3361 tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
     3421 ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
     3481 gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg
     3541 aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc
     3601 ttcccgcaaa cgacccctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag
     3661 ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg
     3721 cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt
     3781 ccacgctaga tctatctcat ctgcgcaagg cagaacgtga agacggccgc cctggacctc
     3841 gcccgcgagc gccaggcgca cgag
//
LOCUS       DAJTYZ010000480         3321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.guided.258, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000480 DAJTYZ010000000
VERSION     DAJTYZ010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.guided.258"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..255)
                     /gene="tetR(A)"
                     /locus_tag="OO596_004585"
     CDS             complement(<1..255)
                     /gene="tetR(A)"
                     /locus_tag="OO596_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR6720188.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARS"
     gene            361..1560
                     /gene="tet(A)"
                     /locus_tag="OO596_004586"
     CDS             361..1560
                     /gene="tet(A)"
                     /locus_tag="OO596_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR6720189.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(1527..1715)
                     /locus_tag="OO596_004587"
                     /pseudo
     CDS             complement(1527..1715)
                     /locus_tag="OO596_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1759..2148)
                     /locus_tag="OO596_004588"
     CDS             complement(1759..2148)
                     /locus_tag="OO596_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720190.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2366..2806
                     /locus_tag="OO596_004589"
     CDS             2366..2806
                     /locus_tag="OO596_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6720191.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT          735 a          909 c          906 g          771 t
ORIGIN      
        1 gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg
       61 cgtatgattc tccgccagca tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct
      121 gaagtgccag taaagcgccg gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt
      181 cagaccgtct acgccgacct cgttcaacag gtccagggcg gcacggatca ctgtattcgg
      241 ctgcaacttt gtcatgcttg acactttatc actgataaac ataatatgtc caccaactta
      301 tcagtgataa agaatccgcg cgttcaatcg gaccagcgga ggctggtccg gaggccagac
      361 gtgaaaccca acagacccct gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc
      421 atcggcctga ttatgccggt gctgccgggc ctcctgcgcg atctggttca ctcgaacgac
      481 gtcaccgccc actatggcat tctgctggcg ctgtatgcgt tgatgcaatt tgcctgcgca
      541 cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc cggtcttgct cgtctcgctg
      601 gccggcgctg ctgtcgacta cgccatcatg gcgacggcgc ctttcctttg ggttctctat
      661 atcgggcgga tcgtggccgg catcaccggg gcgactgggg cggtagccgg cgcttatatt
      721 gccgatatca ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag cgcctgtttc
      781 gggttcggga tggtcgcggg acctgtgctc ggtgggctga tgggcggttt ctccccccac
      841 gctccgttct tcgccgcggc agccttgaac ggcctcaatt tcctgacggg ctgtttcctt
      901 ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct caacccgctc
      961 gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc ggtcttcttc
     1021 atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg cgaggatcgc
     1081 tttcactggg acgcgaccac gatcggcatt tcgcttgccg catttggcat tctgcattca
     1141 ctcgcccagg caatgatcac cggccctgta gccgcccggc tcggcgaaag gcgggcactc
     1201 atgctcggaa tgattgccga cggcacaggc tacatcctgc ttgccttcgc gacacgggga
     1261 tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat gccggcgctg
     1321 caagcaatgt tgtccaggca ggtggatgag gaacgtcagg ggcagctgca aggctcactg
     1381 gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg
     1441 gcttctataa caacgtggaa cgggtgggca tggattgcag gcgctgccct ctacttgctc
     1501 tgcctgccgg cgctgcgtcg cgggctttgg agaaattctt caaattcccg ttgcacatag
     1561 cccggcaatt cctttccctg ctctgccata agcgcagcga atgccgggta atactcgtca
     1621 acgatctgat agagaagggt ttgctcgggt cggtggctct ggtaacgacc agtatcccga
     1681 tcccggctgg ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc aatactgtgt
     1741 ttacatacag tctatcgctt agcggaaagt tcttttaccc tcagccgaaa tgcctgccgt
     1801 tgctagacat tgccagccag tgcccgtcac tcccactctt acattgttcg cagtatctag
     1861 gtacatgtta ttgcgtgcac cttcatgatg tataatgtta ttaatgacag cagaaagatc
     1921 gacagaatta atattcttcg tacctgggac agttatcggg tgtcggccag tgatgagacc
     1981 aaaggatgca gtataagatg aaccactact attatcatgg ccaccattac caccaccgtt
     2041 aaaactacct cccgaacttc cagagccgct atgaccatca ttgtaggggt catttggtcc
     2101 catgtgacca aaaccaccga cagtaccgct atttaactct gtactcataa aattatcctc
     2161 tttgactttt aaaaacagca aaaactgtat ttataacaat gtgtttatat acagtattcg
     2221 aaatactacg cccgtcaaaa ccacaccgtc aacaggtttt ttgtgtattt ttcacgcctc
     2281 acgggcagcc tcagaccttt tgtgttaaaa cagtaatatg ttctgctgtt acaatacggt
     2341 acacacagga gacactgtca cttttatggc atggcacaca gttcaagaga cactgaaatt
     2401 aacaggaaag tcgcgctcgc aattatatcg tgacatggct aaaggccttg tcagctatcg
     2461 cacaggaaaa aatgatcgcc gggagttcga gaccagtgaa ctgattcggg cctatggcga
     2521 gctgcgcact tctgagacac caaaaaggca caaggaggga cactccaaat ctgtcgttag
     2581 ccaggcggaa aatgctcagc tagacgtcat tcagcagaaa ctggacgctc tgcagcagac
     2641 tgttacccta atgcttgaag acaagatggc gcgggaagca gaaaaccgcc tgcgaaatga
     2701 ggagagggaa caactacagg ccgaaattag ccgtctggct gacgcgctgg cacaggaaaa
     2761 aaagcgggga ttctggtcgc ggctgtttcg ctccggttca gagtaacagt gcaggtgact
     2821 ctgatttacc ataacatctg ttatcagcac cgcctgagag gccctcaggg cgtttctgac
     2881 gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct
     2941 gtttttacgg catgcatgag tatgcgcaca agtgcatagc gcggaaattc acaccattca
     3001 gccggggtaa gtcctgtttc cggcgttctt cccatccgaa aacggtgaac ggggacgcaa
     3061 tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aacccgctgt cagcacgttc
     3121 aggaaggggg tgatgtacaa atggcacaga ggatgaaagt gacgtatgag ctgaaggaat
     3181 gatgtaaaag tgatgtataa gtgatgtaaa agtgatgtat aagtgatgta tgaactgaag
     3241 gagcgatgta attctgtagt caggaagaat aacccgttca tgcaggcatg aacgatacag
     3301 tgaggaacgc tttttttgtc a
//
LOCUS       DAJTYZ010000481         3309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466828-rid15815763.guided.259, whole genome shotgun
            sequence.
ACCESSION   DAJTYZ010000481 DAJTYZ010000000
VERSION     DAJTYZ010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466828
            Sequence Read Archive: ERR10074694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477360.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477360.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:49:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,508
            Genes (coding)                    :: 4,212
            CDSs (with protein)               :: 4,212
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 4 (16S, 23S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 136 of 296
            Pseudo Genes (incomplete)         :: 99 of 296
            Pseudo Genes (internal stop)      :: 98 of 296
            Pseudo Genes (multiple problems)  :: 35 of 296
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466828-rid15815763.guided.259"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-07"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            49..522
                     /locus_tag="OO596_004590"
     CDS             49..522
                     /locus_tag="OO596_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6720192.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            617..1141
                     /gene="sat2"
                     /locus_tag="OO596_004591"
     CDS             617..1141
                     /gene="sat2"
                     /locus_tag="OO596_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6720193.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1199..1987
                     /gene="aadA1"
                     /locus_tag="OO596_004592"
     CDS             1199..1987
                     /gene="aadA1"
                     /locus_tag="OO596_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6720194.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            2063..2560
                     /locus_tag="OO596_004593"
     CDS             2063..2560
                     /locus_tag="OO596_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720195.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            2621..2992
                     /locus_tag="OO596_004594"
     CDS             2621..2992
                     /locus_tag="OO596_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6720196.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
BASE COUNT          907 a          780 c          833 g          789 t
ORIGIN      
        1 caaaacgagc atgcttacta ataaaatgtt aacctctgag gaagaattgt gaaactatca
       61 ctaatggtag ctatatcgaa gaatggagtt atcgggaatg gccctgatat tccatggagt
      121 gccaaaggtg aacagctcct gtttaaagct attacctata accaatggct gttggttgga
      181 cgcaagactt ttgaatcaat gggagcatta cccaaccgaa agtatgcggt cgtaacacgt
      241 tcaagtttta catctgacaa tgagaacgta ttgatctttc catcaattaa agatgcttta
      301 accaacctaa agaaaataac ggatcatgtc attgtttcag gtggtgggga gatatacaaa
      361 agcctgatcg atcaagtaga tacactacat atatctacaa tagacatcga gccggaaggt
      421 gatgtttact ttcctgaaat ccccagcaat tttaggccag tttttaccca agacttcgcc
      481 tctaacataa attatagtta ccaaatctgg caaaagggtt aacaagtggc agcaacggat
      541 tcgcaaacct gtcacgcctt ttgtaccaaa agccgcgcca ggtttgcgat ccgctgtgcc
      601 aggcgttagg cgtcatatga agatttcggt gatccctgag caggtggcgg aaacattgga
      661 tgctgagaac catttcattg ttcgtgaagt gttcgatgtg cacctatccg accaaggctt
      721 tgaactatct accagaagtg tgagccccta ccggaaggat tacatctcgg atgatgactc
      781 tgatgaagac tctgcttgct atggcgcatt catcgaccaa gagcttgtcg ggaagattga
      841 actcaactca acatggaacg atctagcctc tatcgaacac attgttgtgt cgcacacgca
      901 ccgaggcaaa ggagtcgcgc acagtctcat cgaatttgcg aaaaagtggg cactaagcag
      961 acagctcctt ggcatacgat tagagacaca aacgaacaat gtacctgcct gcaatttgta
     1021 cgcaaaatgt ggctttactc tcggcggcat tgacctgttc acgtataaaa ctagacctca
     1081 agtctcgaac gaaacagcga tgtactggta ctggttctcg ggagcacagg atgacgccta
     1141 acaattcatt caagccgaca ccgcttcgcg gcgcggctta attcaggagt taaacatcat
     1201 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     1261 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     1321 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     1381 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     1441 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     1501 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     1561 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     1621 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     1681 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     1741 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     1801 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     1861 gcccgtcata cttgaagcta ggcaggctta tcttggacaa gaagatcgct tggcctcgcg
     1921 cgcagatcag ttggaagaat ttgttcacta cgtgaaaggc gagatcacca aggtagtcgg
     1981 caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     2041 cgttagagag ctggggaaga ctatgcgcga tctgttgaag gtggttctaa gcctcgtact
     2101 tgcgatggca tcggggcagg cacttgctga cctgccaatt gttttagtgg atgaagctcg
     2161 tcttccctat gactactccc catccaacta cgacatttct ccaagcaact acgacaactc
     2221 cataagcaat tacgacaata gtccatcaaa ttacgacaac tctgagagca actacgataa
     2281 tagttcatcc aattacgaca atagtcgcaa cggaaatcgt aggcttatat atagcgcaaa
     2341 tgggtctcgc actttcgccg gctactacgt cattgccaac aatgggacaa cgaacttctt
     2401 ttccacatct ggcaaaagga tgttctacac cccaaaaggg gggcgcggcg tctatggcgg
     2461 caaagatggg agcttctgcg gggcattggt cgtcataaat ggccaatttt cgcttgccct
     2521 gacagataac ggcctgaaga tcatgtatct aagcaactag cctgctctct aataaaatgt
     2581 taggcctcaa catctagtcg caagctgagg ggaaccacta gtgtcatacg aacctccaag
     2641 agacggttac acaaacgggt acattgttga tgtcatgtat gacaatcgcc caagtaagta
     2701 tccagctgtg ttcagaacgt acgtccgaat tccagactat gtcgaactag ataacgcgga
     2761 ggagctggcc gacgcaatca ttcgagaggt tgagttaaag gaggcatggc tcacaagggt
     2821 cttggggaac tcagccgatc ctgacgcttc tgtgggcaag tgggaccggc gcctagcgca
     2881 gtgcggtggt attgatgaga tgtcaagtgg ggtgcaggca acatacttca gtccaaacgg
     2941 tcagctggtc ggcttagtgg catacattga tgccgaatgg atggatgaat agctctgacg
     3001 ttacgattca ggtgagacaa ccagatgggc attgcgtaca agctcagttg ccaccgatgt
     3061 ggctacgagt ctgatcttct ttacctaggc caaggtatgg cgatgcttcc tgagcaaata
     3121 gtcggaacgt gcacatgctg cgacaatcta acgacgattt ctataaatga aagctacgga
     3181 gtatgctcac tttgtggggc taaggatcgt gtgatattta caagctatca gcatgaggca
     3241 aggccaagaa atccctgcta tctagagaga agtagactgg ccccctgaat ctccagacaa
     3301 ccagtatca
//
